Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1.
OBJECTIVE
To evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B(1) (FB(1)) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology.


RESULTS
Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (g l(-1)) fermentable sugars provided by sugar cane molasses (CMs), yeast extract (YE) and (NH(4))(2) HPO(4) (DAP) was formulated. The S. cerevisiae RC016 strain showed the greatest binding at all assayed FB1 concentration. The CMs, YE, DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC016.


CONCLUSIONS
A combination of CMs 17%; YE 4·61 g l(-1) and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC016.


CONCLUSIONS
The importance of this work lies in the search for live strains with both probiotic and fumonisin B1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Agencia Córdoba Ciencia (ACC), Ciudad autónoma de Buenos Aires, Argentina.
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Armando
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Galvagno
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Dogi
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Cerrutti
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Dalcero
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Cavaglieri
D000821:Animal Feed; D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D037341:Fumonisins / Q000737:chemistry*; D007218:Industrial Microbiology; D015233:Models, Statistical; D008965:Molasses; D009584:Nitrogen / Q000378:metabolism; D019936:Probiotics; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D031786:Saccharum
D003470:Culture Media; D037341:Fumonisins; C056933:fumonisin B1; D002244:Carbon; D009584:Nitrogen