Noninvasive urine-derived cell lines derived from neurological genetic patients.
Many major inherited neurological disorders are characterized by early childhood onset, high lethality rate, and the absence of effective treatments. A poor understanding of the underlying mechanisms of such disorders is partly because of the scarcity of patient-specific samples. In this study, we cultured the urine sediments of such patients, aiming to explore the capacity of urine cell cultures to obtain specimens from patients suffering from rare inherited neurological diseases. We collected fresh urine from a variety of neurogenetic patients; cultured the specimens; generated different urine cell lines; and classified these cell lines through morphology, reverse transcription-PCR, and immunofluorescence. We then used these cell lines to detect the affected genes in spinal muscular atrophy and Duchenne muscular dystrophy. We successfully established cell lines from patients with spinal muscular atrophy, Duchenne muscular dystrophy, paroxysmal kinesigenic dyskinesia, and Wilson's disease. All established cell lines consisted of urinary tract epithelial cells and podocytes, and had the same gene defects as the blood specimens. Urine cell culture is thus a new, simple, and noninvasive avenue for getting patient-specific samples not only for genetic diagnosis, but also for storing the samples from patients with rare neurological inherited diseases.
Department of Neurology and Institute of Neurology, First Affiliated Hospital, Fuzhou, China.
Qi-Jie
QJ
Zhang
Jin
J
He
Wang
W
Ni
Xiang
X
Lin
Xiang-Ping
XP
Yao
Min-Ting
MT
Lin
Shen-Xing
SX
Murong
Ning
N
Wang
Wan-Jin
WJ
Chen
D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009422:Nervous System Diseases / Q000235:genetics / Q000652:urine*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014556:Urine / Q000166:cytology*