Perfluorocarbon-loaded lipid nanocapsules as oxygen sensors for tumor tissue pO₂ assessment.
The assessment of tumor oxygenation is a crucial factor in cancer therapy and may be carried out using fluorine MRI once fluorine probes have been distributed within the tumor. However, the deposit of those highly fluorinated compounds often jeopardizes anatomical image quality and requires emulsification of the probes. Due to the high density and the high lipophilicity of perfluorocarbons, nanoemulsion of these molecules usually requires high-energy processes. In the present work, we discuss the synthesis and the physico-chemical characterization of perfluorocarbon nanocapsules using a low-energy phase-inversion process. The nanocapsules were tested on a mouse tumor brain model to assess oxygenation.
INSERM U 1066, Micro et Nanomédecines biomimétiques - MINT, Angers, France. laurent.lemaire@univ-angers.fr
L
L
Lemaire
G
G
Bastiat
F
F
Franconi
N
N
Lautram
T
T
Duong Thi Dan
E
E
Garcion
P
P
Saulnier
J P
JP
Benoit
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002138:Calibration; D045744:Cell Line, Tumor; D005461:Fluorine / Q000737:chemistry; D005462:Fluorine Radioisotopes / Q000494:pharmacology; D005466:Fluorocarbons / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D016513:Mice, SCID; D053769:Nanocapsules / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D005462:Fluorine Radioisotopes; D005466:Fluorocarbons; D008055:Lipids; D053769:Nanocapsules; D005461:Fluorine; D010100:Oxygen