In vivo investigation of ceftiofur-loaded gelatin and PLGA microspheres in beagle dogs.
Drug delivery systems based on polymer microspheres have received considerable attention. Ceftiofur sodium and ceftiofur hydrochloride is widely used for the treatment of bacterial diseases in animals but the delivery in vivo has not been reported. In this paper, we report the synthesis of microspheres from gelatin and PLGA, two kinds of typical natural and artificial materials, for loading ceftiofur and the in vivo investigation of the pharmacokinetics in beagle dogs. By controlling the synthesis parameters, gelatin and PLGA microspheres with diameter between 5 and 35 microns were obtained. Assay procedures based on high performance liquid chromatography were evaluated and confirmed. The dogs were randomly divided into three groups, i.e., control group, gelatin group, and PLGA group and administrated via intravenous injection. Plasma concentrations of ceftiofur over time were measured and analyzed. Results indicate that the main kinetic parameters do not show significant difference for the gelatin group and control group, but the area under the curve, plasma half-life, apparent volume of distribution, and clearance ratio of PLGA group show significant difference from the gelatin group and the control group. The PLGA microspheres show a low area under the curve but long time release.
Laboratories of Biological Pharmaceutical, College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, China.
Zhihui
Z
Hao
Leilei
L
Wang
Kefeng
K
Xiao
Yongda
Y
Zhao
Ming
M
Zou
Qidi
Q
Zhang
Zhaopeng
Z
Ding
Fenfang
F
Yang
Baohan
B
Qu
D000818:Animals; D002511:Cephalosporins / Q000008:administration & dosage*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005780:Gelatin / Q000008:administration & dosage*; D019344:Lactic Acid / Q000008:administration & dosage*; D008297:Male; D008863:Microspheres; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
D002511:Cephalosporins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C053503:ceftiofur; D005780:Gelatin