Effect of grinding intensity and feed physical form on in vitro adhesion of Salmonella Typhimurium and mannose residues in intestinal mucus receptors for salmonellae.
The hypothesis of this study was that feeding a fine, pelleted diet (FP) compared to a coarse meal diet (CM) results in a higher mannose content in the intestinal mucus of pigs and therefore an increased in vitro adhesion of Salmonella Typhimurium DT104 L to the mucus. The 2 diets were fed to a total of 24 weaned pigs for 6 wk after which mannose content in the mucus was evaluated histochemically using the α1-3-d-mannose-specific lectin Galanthus nivalis agglutinin. The crypt width was determined as an indirect measure for the amount of secreted mucus. Ileal and cecal tissue samples were incubated with approximately 7.77 × 10(7) cfu Salmonella Typhimurium and numbers of salmonellae adhering to the mucus and/or mucosa were determined by culture techniques. There was no effect of feed physical form on the in vitro adhesion of S. Typhimurium either in the ileum (7.1 ± 0.19 log(10) cfu/g tissue) or in the cecum (6.8 ± 0.26 log(10) cfu/g). The mannose content of the mucus also did not differ between the treatment groups. The crypts of the duodenum, jejunum, and cecum were wider (P < 0.05) after feeding the CM diet. This might be an indication for a higher mucus production in these pigs.
Institute of Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
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Callies
S J
SJ
Sander
J
J
Verspohl
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A
Beineke
J
J
Kamphues
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D004032:Diet / Q000662:veterinary; D005511:Food Handling; D007422:Intestines / Q000502:physiology*; D008358:Mannose / Q000737:chemistry*; D009093:Mucus / Q000378:metabolism; D011956:Receptors, Cell Surface; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012486:Salmonella typhimurium; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000517:prevention & control
D011956:Receptors, Cell Surface; D008358:Mannose