[Identification and genetic characterization of coxsackievirus A24 isolated from patients with acute hemorrhagic conjunctivitis in Shandong Province].
To identify the pathogen of acute hemorrhagic conjunctivitis (AHC) in Shandong Province in 2010, eye mucous swab samples were collected from 26 patients in Qingdao and Linyi City. Real time-PCR assays for EV70, CVA24 and Adenovirus were performed on these samples. The result showed 17 samples (65.39%) were CVA24 positive while all the samples for HEV70 and Adenovirus detection were negative, which implied that CVA24 was the causative pathogen of this outbreak. A total of 10 virus strains isolated on Hep-2 cells were identified as CVA24 through VP1 amplification and nucleotide sequence analysis. The nucleotide and amino acid homologies on VP1 region among these isolates were 99.3%-100.0% and 99.5%-100.0%, respectively, and the strains aggregated together to one clade in phylogenetic tree. These results showed that the CVA24 circulating in Qingdao and Linyi City belonged to one transmission chain. Shandong CVA24s segregated into 5 different clades, and great nucleotide divergence was observed be tween AHC isolates and others.
School of Public Health, Shandong University, Jinan 250012, China. yangjingdn@sina.com
Jing
J
Yang
Yi
Y
Lin
Hai-Yan
HY
Wang
Ze-Xin
ZX
Tao
Yan
Y
Li
Peng
P
Chen
Yao-Wen
YW
Pei
Feng
F
Ji
Xiao-Juan
XJ
Lin
Xian-Jun
XJ
Wang
Yao
Y
Liu
Ai-Qiang
AQ
Xu
D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D003232:Conjunctivitis, Acute Hemorrhagic / Q000453:epidemiology / Q000821:virology*; D030015:Enterovirus C, Human / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins