Non-linear optical microscopy of kidney tumours.
The unregulated cancer cell growth leads to strong alterations in morphology and composition of the tissue. The combination of coherent anti-Stokes Raman scattering, two-photon excited fluorescence and second harmonic generation enables a high resolution imaging with strong information on tissue composition and can then provide useful information for tumour diagnosis. Here we present the potential of multimodal non-linear microscopy for imaging of renal tumours. Using cryosections of human oncocytoma and carcinoma, the method gave a detailed insight in cancer morphology and composition, enabling to discern between normal kidney tissue, tumour and necrosis. Several features significant for the diagnosis were clearly visualised without use of any staining. Translation of this method in clinical pathology will greatly improve speed and quality of the analyses.
Dresden University of Technology, Faculty of Medicine, Clinical Sensing and Monitoring, Fetscher Str. 74, D-01307 Dresden, Germany. roberta.galli@tu-dresden.de.
Roberta
R
Galli
Valdas
V
Sablinskas
Darius
D
Dasevicius
Arvydas
A
Laurinavicius
Feliksas
F
Jankevicius
Edmund
E
Koch
Gerald
G
Steiner
D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D008853:Microscopy / Q000379:methods*; D009392:Nephrectomy; D017711:Nonlinear Dynamics; D055620:Optical Phenomena; D013059:Spectrum Analysis, Raman; D013194:Staining and Labeling
CARS; SHG; TPEF; cancer; kidney; label-free; non-linear microscopy