Radiosynthesis and initial evaluation of (18)F labeled nanocarrier composed of poly(L-lactic acid)-block-poly(sarcosine) amphiphilic polydepsipeptide.
BACKGROUND
With the aim of developing radiotracers for in vivo positron emission tomography (PET) imaging of solid tumors based on the enhanced permeability and retention effect of nanocarriers, we have developed a polymer micelle named "Lactosome", which is composed of the amphiphilic polydepsipeptide, poly(L-lactic acid)-block-poly(sarcosine). This paper describes and evaluates the initial evaluation of the (18)F-labeled Lactosome as a novel contrast agent for the tumor PET imaging technique carried out.


METHODS
(18)F-labeled Lactosomes were prepared by a film hydration method under sonication in water at 50°C from a mixture of 4-[(18)F]fluoro-benzoyl poly-L-lactic acid ((18)F-BzPLLA30) and the amphiphilic polydepsipeptide. For biodistribution studies, BALB/cA Jcl-nu/nu mice bearing HeLa cells in the femur region were used. We took both PET and near-infrared fluorescence (NIRF) images of tumor bearing mice after co-injection of (18)F-labeled Lactosome and NIRF-labeled Lactosome.


RESULTS
(18)F-labeled Lactosomes were prepared at good yields (222-420MBq) and more than 99% of (18)F-BzPLLA30 was incorporated into (18)F-labeled Lactosome. The radioactivity of (18)F-labeled Lactosome was found to be stable and maintained at high level for up to 6h after injection into the blood stream. Tumor uptake increased gradually after the injection. The uptake ratio of tumor/muscle was 2.7 at 6h from the time of injection. Tumor PET imaging with (18)F-labeled Lactosome was as capable as tumor NIRF imaging with NIRF-labeled Lactosome.


CONCLUSIONS
Tumor PET imaging using Lactosome as a nanocarrier may be therefore a potential candidate for a facile and general solid tumor imaging technique.
Department of Radiopharmacy, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, 981-8558, Japan. fyamamot@tohoku-pharm.ac.jp
Fumihiko
F
Yamamoto
Ryo
R
Yamahara
Akira
A
Makino
Kensuke
K
Kurihara
Hideo
H
Tsukada
Eri
E
Hara
Isao
I
Hara
Shinae
S
Kizaka-Kondoh
Yasuhito
Y
Ohkubo
Eiichi
E
Ozeki
Shunsaku
S
Kimura
D000818:Animals; D047630:Depsipeptides / Q000737:chemistry*; D005260:Female; D005462:Fluorine Radioisotopes; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008823:Micelles; D049329:Nanostructures / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D049268:Positron-Emission Tomography; D011854:Radiochemistry
D047630:Depsipeptides; D005462:Fluorine Radioisotopes; D008823:Micelles; D011091:Polyesters; C578344:poly(lactic acid)-b-poly(sarcosine)