Novel ssDNA virus recovered from estuarine Mollusc (Amphibola crenata) whose replication associated protein (Rep) shares similarities with Rep-like sequences of bacterial origin.
Over the past couple of years highly diverse novel ssDNA viruses have been discovered. Here, we present the first ssDNA virus, Gastropod-associated circular ssDNA virus (GaCSV), recovered from a mollusc Amphibola crenata Martyn 1784, which is a deposit feeder that grazes micro-organisms and organic detritus on the surface of tidal mudflats. The GaCSV (2351 nt) genome contains two large bidirectionally transcribed ORFs. The smaller ORF (874 nt) has similarities to viral replication-associated protein (Rep) sequences of some bacteria and circoviruses, whereas the larger ORF (955 nt) does not relate to any sequences in public databases and we presume it potentially encodes the capsid protein. Phylogenetic analysis shows that the GaCSV Rep clusters with Rep-like sequences of bacterial origin, highlighting the role of ssDNA viruses in horizontal gene transfer. The occurrence of previously unknown viruses in organisms associated with human pollution is a relatively unexplored field.
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Anisha
A
Dayaram
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Sharyn
S
Goldstien
Centre for Freshwater Management, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.|Department of Geography, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Peyman
P
Zawar-Reza
Natural hazards research centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.|Centre for Freshwater Management, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.|Department of Geography, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Christopher
C
Gomez
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Jon S
JS
Harding
Electron Microscope Unit, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.|Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.|School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Arvind
A
Varsani
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004267:DNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004277:DNA, Single-Stranded / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D063366:Estuaries; D049851:Gastropoda / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics; D053833:Wetlands
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012989:Soil Pollutants; D014764:Viral Proteins