Single whole blood dextran sulfate adsorption favorably affects systemic oxidative balance in lipoprotein apheresis patients.
OBJECTIVE
The acute changes of circulating oxidative stress parameters were compared in 16 patients undergoing two different apheresis techniques: plasma lipidfiltration (LF) or whole blood dextran sulfate adsorption (DSA).


RESULTS
Immediately after apheresis LF was associated with an increase in systemic phagocyte count, enhanced formation of reactive oxygen species and decreased activity of the antioxidant enzyme paraoxonase. After DSA, circulating phagocyte oxidant generating activity was significantly lower. Compared to LF, the systemic level of oxidized LDL and antioxLDL antibodies showed a larger decrease in DSA. All measured oxidative stress parameters returned to nearly pre-apheresis level at day three after apheresis,


CONCLUSIONS
The data show a more pronounced leukocyte activation immediately after LF in contrast to DSA, possibly as a consequence of necessity of prior separation of blood plasma. The pathophysiological importance of the short-term oxidative burden after a single apheresis session remains to be determined.
Department of Internal Medicine III, University Hospital Carl Gustav Carus, Dresden, Germany. Steffi.Kopprasch@uniklinikum-dresden.de
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Kopprasch
S R
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Bornstein
P E H
PE
Schwarz
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Bergmann
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Julius
J
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Graessler
D000327:Adsorption; D000368:Aged; D000704:Analysis of Variance; D043303:Aryldialkylphosphatase / Q000097:blood; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001781:Blood Component Removal / Q000009:adverse effects / Q000379:methods*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D016264:Dextran Sulfate / Q000009:adverse effects / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D017270:Lipoprotein(a) / Q000097:blood; D008074:Lipoproteins / Q000097:blood* / Q000276:immunology; D008077:Lipoproteins, LDL / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010586:Phagocytes / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000097:blood; D013997:Time Factors; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood
D001323:Autoantibodies; D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D017270:Lipoprotein(a); D008074:Lipoproteins; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D016264:Dextran Sulfate; D043303:Aryldialkylphosphatase