CD271(+) bone marrow mesenchymal stem cells may provide a niche for dormant Mycobacterium tuberculosis.
Mycobacterium tuberculosis (Mtb) can persist in hostile intracellular microenvironments evading immune cells and drug treatment. However, the protective cellular niches where Mtb persists remain unclear. We report that Mtb may maintain long-term intracellular viability in a human bone marrow (BM)-derived CD271(+)/CD45(-) mesenchymal stem cell (BM-MSC) population in vitro. We also report that Mtb resides in an equivalent population of BM-MSCs in a mouse model of dormant tuberculosis infection. Viable Mtb was detected in CD271(+)/CD45(-) BM-MSCs isolated from individuals who had successfully completed months of anti-Mtb drug treatment. These results suggest that CD271(+) BM-MSCs may provide a long-term protective intracellular niche in the host in which dormant Mtb can reside.
Division of Oncology, Departments of Medicine and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA. bikuldas@stanford.edu
Bikul
B
Das
Suely S
SS
Kashino
Ista
I
Pulu
Deepjyoti
D
Kalita
Vijay
V
Swami
Herman
H
Yeger
Dean W
DW
Felsher
Antonio
A
Campos-Neto
D000071916:AC133 Antigen; D000336:Aerosols; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001853:Bone Marrow / Q000382:microbiology / Q000473:pathology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D050296:Microbial Viability; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D055153:Stem Cell Niche; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology
D000071916:AC133 Antigen; D000336:Aerosols; D015703:Antigens, CD; D006023:Glycoproteins; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D010455:Peptides; D017475:Receptors, Nerve Growth Factor