Synthesis and spectroscopic properties of fluorescent 5-benzimidazolyl-2'-deoxyuridines 5-fdU probes obtained from o-phenylenediamine derivatives.
Fluorescent nucleosides (dU(bmz)) with desirable fluorescence quantum yield (Φ) are synthesized from almost non-fluorescent 5-fdU and o-phenylenediamine derivatives. The fluorescence of these nucleosides is quite sensitive to pH and organic solvents. 4-Methoxybenzene-1,2-diamine was used for the detection of 5-fdU among natural nucleosides.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan, 430072, PR China.
Pu
P
Guo
Xiaowei
X
Xu
Xiaoyu
X
Qiu
Yimin
Y
Zhou
Shengyong
S
Yan
Changcheng
C
Wang
Chunjiang
C
Lu
Wen
W
Ma
Xiaocheng
X
Weng
Xianzheng
X
Zhang
Xiang
X
Zhou
D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D015394:Molecular Structure; D010655:Phenylenediamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D010655:Phenylenediamines; C034193:1,2-diaminobenzene; D003857:Deoxyuridine