A new microfluidic device for electric lysis and separation of cells.
This paper demonstrates the potential use of a new microfluidic device embedding thick electrodes for cell lysis and cell separation applications. The system consists of a microfluidic channel featuring conductive walls made of a polydimethylsiloxane (PDMS) matrix mixed with carbon nanoparticles. Cell lysis was performed electrically by applying square pulses across the channel width, which was monitored by fluorimetry. Lysed and unlysed cells showed different dielectrophoretic behavior under appropriate experimental conditions, which suggests that the developed device is suitable to perform both cell lysis and subsequent sorting of viable and dead cells.
Ampère Lab, UMR5005 CNRS, Ecole Centrale de Lyon, 39134 Ecully Cedex, France. mathieu.brun@ec-lyon.fr
M
M
Brun
M
M
Frénéa-Robin
J F
JF
Chateaux
N
N
Haddour
A L
AL
Deman
R
R
Ferrigno
D002469:Cell Separation / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes; D004586:Electrophoresis / Q000379:methods; D018274:Electroporation; D005470:Fluorometry; D057809:HEK293 Cells; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation*
D004129:Dimethylpolysiloxanes; C013830:baysilon