Consumption of guar gum and retrograded high-amylose corn resistant starch increases IL-10 abundance without affecting pro-inflammatory cytokines in the colon of pigs fed a high-fat diet.
Increases in dietary intake of viscous and nonviscous soluble fiber are reported to improve bowel health. However, related biological mechanisms are not very clear. This study was conducted to examine if colonic inflammation would occur in a typical Western diet model and determine if consumption of soluble fiber components would attenuate potential detrimental effects by differentially affecting colonic abundances of anti-inflammatory cytokine IL-10 and 2 pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and IL-6 in pigs fed a high-fat basal diet supplemented, respectively, with 15% viscous soluble fiber guar gum (GG) and 15% nonviscous soluble fiber, that is, retrograded high-amylose corn (Zea mays) resistant starch (RS). A total of 24 Yorkshire growing barrows were assigned into a standard corn and soybean (Glycine max) meal (SBM)-based grower diet as a positive control (PC), an animal protein-based high-fat basal diet as the negative control (NC), and 2 NC basal diets supplemented with 15% GG and 15% RS, respectively, according to a completely randomized block design for 4 wk. Abundance of these cytokines in homogenized and extracted colonic tissue supernatant samples was measured by ELISA. Although colonic IL-10 abundance was lower (P < 0.05) in the corn and SBM-based PC group than that in the high-fat basal NC group, there were no differences (P > 0.05) in colonic abundances of TNF-α and IL-6 between NC and PC groups and among all of the treatment groups. Compared with the NC group, consumption of GG and RS at 15% increased (P < 0.05) colonic IL-10 abundance. Moreover, there was no difference (P > 0.05) in colonic IL-10 abundance between the 15% GG and the 15% RS groups. Thus, consumption of a typical high-fat Western diet did not induce colonic inflammation. Diets supplemented with 15% GG or 15% RS may protect the colon from developing inflammation by enhancing IL-10 abundance.
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada. mfan@uoguelph.ca
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D000688:Amylose / Q000737:chemistry; D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D003106:Colon / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D004041:Dietary Fats / Q000494:pharmacology*; D005685:Galactans / Q000737:chemistry* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008351:Mannans / Q000737:chemistry* / Q000494:pharmacology; D053149:Plant Gums / Q000737:chemistry* / Q000494:pharmacology; D013213:Starch / Q000737:chemistry*; D013552:Swine / Q000502:physiology*; D059467:Transcriptome; D003313:Zea mays / Q000737:chemistry
D004041:Dietary Fats; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D016753:Interleukin-10; D013213:Starch; D000688:Amylose; C007894:guar gum