Quantum dot-assembled nanoparticles with polydiacetylene supramolecule toward label-free, multiplexed optical detection.
Quantum dot (QD)-assembled silica nanoparticles bearing a polydiacetylene (PDA) supramolecule on their surface (SiO(2)@QDs@PDA NPs) were developed for label-free and multiplexed detection of biological molecules. Two types of QD-assembled silica NPs (SiO(2)@QDs NPs) were prepared and coated with the PDA supramolecule via photo-induced polymerization of 10,12-pentacosadiynoic acid. One of the SiO(2)@QDs NPs was embedded with blue-QDs, and the other was embedded with green-QDs for encoding. The resulting SiO(2)@QDs@PDA NPs showed discrete QD photoluminescence for encoding as well as PDA fluorescence for sensing a target without interference or overlap. Under heating stress of the SiO(2)@QDs@PDA NPs, the color of the PDA changed from blue to red, which allowed us to observe the fluorescence emitted from red PDA. The mixture of two different SiO(2)@QDs@PDA NPs, SiO(2)@QDs@blue-PDA NPs not emitting the fluorescence of PDA and SiO(2)@QDs@red-PDA NPs where stress was brought onto turn on the PDA fluorescence, was effectively imaged and readily distinguished via fluorescence microscopy, indicating their potential for label-free and multiplexed detection of target molecules.
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
San
S
Kyeong
Homan
H
Kang
Joonhyuk
J
Yim
Su-Ji
SJ
Jeon
Cheol Hwan
CH
Jeong
Yoon-Sik
YS
Lee
Bong-Hyun
BH
Jun
Jong-Ho
JH
Kim
D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry*
D005456:Fluorescent Dyes; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D012822:Silicon Dioxide