[Preliminary study on molluscicidal effect of Euphorbia helioscopia L ethanol extracts against Oncomelania hupensis].
OBJECTIVE
To study the influences of Euphorbia helioscopia L. on glycogen contents of Oncomelania hupensis so as to explore its molluscicidal mechanism.


METHODS
The different polar factions of Euphorbia helioscopia L. were separated by using the systemic solvent segregation method, and then its molluscicidal activity was detected according to the Laboratory Final Milluscicides Screening Method issued by WHO. The glycogen contents of soft tissues of Oncomelania hupensis snails treated by Euphorbia helioscopia L. ethanol extracts were determined by the anthrone method.


RESULTS
After being immersed for 48 h with Euphorbia helioscopia L. ethanol extracts, the death rates of the snails at the concentrations of 400 mg/L and 800 mg/L were 50.43% and 77.50% respectively, while after 96 h, the death rates at the above concentrations were both 100%. The glycogen contents of the snails gradually reduced with the increase of the drug concentrations, the glycogen content decreased to the lowest while the concentration was 800 mg/L, and the glycogen content decreased by 69.49% compared with that of the control group.


CONCLUSIONS
The Euphorbia helioscopia L. ethanol extracts can significantly decrease the glycogen content of Oncomelania snails and the extracts have an adequate molluscicidal activity.
Department of Pathogenic Biology, School of Basic Medicine and Bioscience, Medical College of Soochow University, Suzhou 215123, China.
Jing
J
Zhang
Chen-Chen
CC
Liu
Xia
X
Zhou
Si-Jia
SJ
Gao
Wei
W
Gong
Hong-Xiang
HX
Zhu-Ge
D000818:Animals; D028481:Euphorbia / Q000737:chemistry*; D008975:Molluscacides / Q000633:toxicity*; D010571:Pest Control; D010936:Plant Extracts / Q000633:toxicity*; D012908:Snails / Q000187:drug effects* / Q000254:growth & development
D008975:Molluscacides; D010936:Plant Extracts