Rat model of intracerebral hemorrhage permitting hematoma aspiration plus intralesional injection.
A combination of hematoma aspiration and local delivery of chemicals may be more effective than either therapy in intracerebral hemorrhage (ICH). The aim of the present study was to develop a rat model of hematoma aspiration plus intralesional injection after ICH. ICH was induced in adult Sprague-Dawley rats by an intrastriatal injection of bacterial collagenase IV. Hematoma aspiration was performed 3.5 h after ICH onset. Following aspiration, normal saline was injected into the lesion cavity. Hematoma aspiration with or without subsequent saline injection significantly reduced the hematoma volume, lesion volume, and perihematomal neutrophil infiltration. Hematoma aspiration plus subsequent intralesional injection is simple, feasible, and safe. This ICH model can be used to assess the effectiveness of hematoma removal plus local delivery of chemicals.
Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Yan-Hua
YH
Sang
Yu-Xiang
YX
Liang
Ling-Guang
LG
Liu
Rutledge G
RG
Ellis-Behnke
Wu-Tian
WT
Wu
Kwok-Fai
KF
So
Raymond Tak-Fai
RT
Cheung
D000818:Animals; D002543:Cerebral Hemorrhage / Q000628:therapy*; D004195:Disease Models, Animal; D015552:Injections, Intralesional; D008297:Male; D003012:Microbial Collagenase / Q000008:administration & dosage*; D019060:Minimally Invasive Surgical Procedures / Q000379:methods*; D009339:Needles; D051381:Rats; D017207:Rats, Sprague-Dawley; D013396:Suction / Q000379:methods*
D003012:Microbial Collagenase