In vitro effect of paediatric liquid medicines on deciduous enamel exposed to biofilm.
OBJECTIVE
This study aimed to assess the in vitro effects of paediatric liquid medicines on deciduous enamel exposed to biofilms.


METHODS
Fragments (n = 25) of first primary molars were covered by nail varnish, leaving a 22 mm(2) exposure area. Specimens were fixed in polystyrene plates containing BHI broth media. Pooled human saliva was added to form a mature biofilm on fragments over a 10-day period in microaerophilic conditions. Specimens were divided into groups (n = 5 per group) and treated (50 μL) daily for 1 min over 1 week as follows: G1 = 10% sucrose solution (positive control); G2 = Dimetapp Elixir® (antihistamine); G3 = Claritin® (antihistamine); and G4 = Klaricid® (antibiotic). Five other fragments, without treatment and inoculum represented the blank controls. The covered area for each specimen represented the negative control. Cross-sectional hardness of the enamel was used as a demineralization indicator.


RESULTS
All treatment groups showed hardness loss compared to the corresponding negative controls (p < 0.05). Among the treatment groups, G2 exhibited the greatest demineralization pattern (p < 0.05) followed by G3, G1 and G4.


CONCLUSIONS
All medicines caused deciduous enamel demineralization in the presence of biofilm. The greatest hardness loss was observed after treatment with Dimetapp Elixir®.
Department of Paediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Daniela Novaes
DN
Soares
Andréa Gonçalves
AG
Antonio
Viviane Santos da Silva
VS
Pierro
Natalia Lopes Pontes
NL
Iorio
Kátia Regina Netto
KR
Dos Santos
Lucianne Cople
LC
Maia
D000284:Administration, Oral; D018441:Biofilms; D002648:Child; D003743:Dental Enamel / Q000187:drug effects*; D004304:Dosage Forms; D064420:Drug-Related Side Effects and Adverse Reactions; D006801:Humans; D066298:In Vitro Techniques; D014094:Tooth, Deciduous / Q000187:drug effects*
D004304:Dosage Forms