Structure of the O-polysaccharide of Edwardsiella tarda PCM 1150 containing an amide of D-glucuronic acid with L-alanine.
Mild acid degradation of the lipopolysaccharide of Edwardsiella tarda PCM 1150 afforded an O-polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectroscopies, including experiments performed in a 9:1 H2O/D2O mixture to detect NH protons and their correlations with CH protons. The O-polysaccharide was found to contain an amide of d-glucuronic acid with l-alanine (d-GlcA6Ala) and the following structure of the branched hexasaccharide repeating unit was established: -->4)-β-D-GlepA6Ala-(1-->4)-α-L-Fucp-(1-->4)-α-D-Glcp-(1-->4)-α-D-Quip-(1-->3)-β-D-GlcpNAc-(1-->3<--1α-D-GalpNAc.
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland. katzenel@iitd.pan.wroc.pl
Ewa
E
Katzenellenbogen
Nina A
NA
Kocharova
Alexander S
AS
Shashkov
Sabina
S
Górska-Frączek
Maria
M
Bogulska
Andrzej
A
Gamian
Yuriy A
YA
Knirel
D000409:Alanine / Q000737:chemistry*; D000577:Amides / Q000737:chemistry*; D002240:Carbohydrate Sequence; D020609:Edwardsiella tarda / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
D000577:Amides; D011134:Polysaccharides; D020723:Glucuronic Acid; D000409:Alanine