Biocompatibility of microbially reduced graphene oxide in primary mouse embryonic fibroblast cells.
Graphene nanosheet is a one-atom thick planar sheet of sp(2)-bonded carbon atoms, which are densely packed in a honeycomb crystal lattice, attracting tremendous attention from both fundamental research and industrial applications. The synthesis of graphene from graphene oxide (GO) using a biological method is one of the important topics in the areas of nanotechnology, because graphene-based nanomaterials have potential applications. A green, simple and non-toxic method for preparing graphene using biomass of Pseudomonas aeruginosa as the reducing reagent is proposed. The resulting microbially reduced graphene oxide (M-rGO) was characterized using a range of analytical techniques. UV-visible spectroscopy confirms the transition of graphene oxide to graphene. Fourier transform infrared spectroscopy (FT-IR) was used to study the changes in surface functionalities. X-ray diffraction (XRD) and high resolution scanning electron microscopy (SEM) were used to investigate the crystalline nature and the morphologies of prepared graphene respectively. Furthermore, the biocompatibility of the M-rGO was investigated using primary mouse embryonic fibroblast (PMEF) cells. The present study suggests that the M-rGO has significant biocompatibility for PMEF cells, even at a high concentration of 100 μg ml(-1). Therefore, the proposed safe and green method confers the M-rGO with a great potential for various biomedical applications.
Department of Animal Biotechnology, Konkuk University, Gwangin-gu, Seoul, Republic of Korea. gsangiliyandi@yahoo.com
Sangiliyandi
S
Gurunathan
Jae Woong
JW
Han
Vasuki
V
Eppakayala
Jin-Hoi
JH
Kim
D000818:Animals; D001672:Biocompatible Materials; D018533:Biomass; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006108:Graphite / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D019163:Reducing Agents / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D010087:Oxides; D019163:Reducing Agents; D006108:Graphite