Evaluation of two external markers for measurement of ileal and total tract digestibility of pigs fed human-type diets.
External markers for determination of nutrient digestibility have often been evaluated in conventional dry feeds but less often in conventional feeds such as human-type diets used in animal model studies. In the present study, 5 ileal-cannulated pigs were fed 5 types of soft bread-based diets supplemented with Cr(2)O(3) and AIA as digestibility markers for 1 wk in a Latin square design. Ileal contents were collected twice for 5 h and a fecal grab sample was obtained once per week. Ileal and total tract digestibility of OM and nonstarch polysaccharides (NSP) based on the 2 markers were compared by linear regression. Across dietary treatments and site of collection, high correlation existed between digestibility values obtained with Cr(2)O(3) and AIA, resulting in a R(2) > 0.77 (P < 0.001) and a linear relation close to unity. For ileal samples, the correlation was poor, particularly for NSP, which had R(2) = 0.09 (P = 0.14) whereas OM had an R(2) = 0.52 (P <0.001). On the other hand, fecal grab samples led to R(2) > 0.92 (P < 0.001) for both OM and NSP. However, AIA gave higher values than Cr(2)O(3), particularly in samples with lower digestibility. The discrepancy is presumably caused by analytical difficulties due to a high fecal ash contents or interference with other components in the human-type diets.
Aarhus University, Department of Animal Science, 8830 Tjele, Denmark. Hellen.laerke@agrsci.dk
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Lærke
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MM
Kasprzak
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Bach Knudsen
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015415:Biomarkers; D001939:Bread; D017608:Chromium Compounds / Q000494:pharmacology; D004032:Diet / Q000662:veterinary*; D004063:Digestion / Q000502:physiology; D006801:Humans; D013552:Swine / Q000502:physiology*
D015415:Biomarkers; D017608:Chromium Compounds; C023600:chromic oxide