Doped TiO2 anodic layers of enhanced antibacterial properties.
Ti-6Al-4V joint replacement implants foster uncemented fixation in orthopaedic surgery. However, bacterial colonization competes with host cells and ultimately may produce implant-related difficult-to-treat infections, justifying the efforts to obtain infection-resistant materials. In a previous work, the authors demonstrated the antibacterial properties of anodic fluoride-TiO2 nanostructured layers on Ti-6Al-4V alloy. In this work, the anodizing bath has been modified in order to grow fluoride-TiO2 barrier layers (FBL). A bacterial adherence protocol, run with reference and six different clinical strains of Staphylococcus aureus and Staphylococcus epidermidis, showed a statistically significant decrease in the percentage of covered surface (p<0.0001, Kruskal-Wallis test) for FBL specimens when compared with non fluoride-containing specimens, i.e. chemically polished Ti-6Al-4V and F-free TiO2 barrier layers. The results obtained on the F-barrier layers allowed discrimination between the effects of the presence of fluoride in the layer and the layer nanostructure on bacterial adhesion.
Centro Nacional de Investigaciones Metalúrgicas (CENIM/CSIC), Madrid, Spain.
María A
MA
Arenas
Concepción
C
Pérez-Jorge
Ana
A
Conde
Endzhe
E
Matykina
Juan M
JM
Hernández-López
Ramón
R
Pérez-Tanoira
Juan J
JJ
de Damborenea
Enrique
E
Gómez-Barrena
Jaime
J
Esteba
D000497:Alloys; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D004566:Electrodes; D005459:Fluorides / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
D000497:Alloys; D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D014025:Titanium; D005459:Fluorides