Production of fumaric acid by immobilized Rhizopus arrhizus on net.
An immobilization method using net was developed for fumaric acid fermentation by Rhizopus arrhizus RH-07-13. The large surface of the net immobilized enough filamentous mycelia which produced fumaric acid rapidly. Net size and spore concentration were optimized to enhance fermentation performance and 150cm(2) of net size, 0.5×10(6)per ml of spore concentration were selected finally. Compared to free-cell fermentation, fumaric acid production was flat (32.03 vs. 31.23g/L) but fermentation time reduced 83.3% (24 vs. 144h).
Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China.
Chunbo
C
Gu
Yuqing
Y
Zhou
Luo
L
Liu
Tianwei
T
Tan
Li
L
Deng
D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005650:Fumarates / Q000302:isolation & purification* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D012233:Rhizopus / Q000302:isolation & purification* / Q000378:metabolism*; D014462:Ultrafiltration / Q000295:instrumentation
D005650:Fumarates; C032005:fumaric acid; D005947:Glucose