Polymorphism of CLOCK gene rs 4580704 C > G is associated with susceptibility of Alzheimer's disease in a Chinese population.
OBJECTIVE
The association of polymorphism of circadian locomotor output cycle kaput (CLOCK) gene rs 4580704 C/G with susceptibility of Alzheimer's disease (AD) was examined in the present study.


METHODS
This was a case/control study and investigated the association of polymorphism of CLOCK gene rs 4580704 C/G with susceptibility of AD. Genotypes of apolipoprotein E (APOE) and CLOCK gene rs 4580704 C/G were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) detection method.


RESULTS
This study was comprised of 296 unrelated AD patients and 423 controls. We performed an analysis the association of polymorphism of CLOCK gene rs 4580704 C/G with susceptibility of AD. In the whole sample or APOEε4 noncarriers, prevalence of C carriers in CLOCK gene rs 4580704 in AD patients was significantly higher than in controls (in the whole sample: χ(2) = 13.773, p <0.0001; in APOEε4 noncarriers: χ(2) = 51.588, p <0.0001). However, among APOEε4 carriers, prevalence of C carriers in CLOCK gene rs 4580704 between patients and controls was not statistically significant (χ(2) = 0.753, p = 0.386).


CONCLUSIONS
Among APOEε4 noncarriers, C carriers in CLOCK gene rs 4580704 were associated with a high susceptibility of AD; however, among APOEε4 carriers the functional polymorphism of clock gene rs 4580704 C/G was not associated with AD susceptibility.
Department of Neurosurgery, West China Hospital, Sichuan University, Sichuan, China.
Hai-feng
HF
Chen
Chang-quan
CQ
Huang
Chao
C
You
Zheng-rong
ZR
Wang
Huang
H
Si-qing
D000368:Aged; D000483:Alleles; D000544:Alzheimer Disease / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D056926:CLOCK Proteins / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D001057:Apolipoproteins E; D056926:CLOCK Proteins; C538943:CLOCK protein, human