High-performance liquid chromatography using pressurized liquid extraction for the determination of seven tetracyclines in egg, fish and shrimp.
A simple and especially rapid method, pressurized liquid extraction, has been developed and applied to the quantitative determination of oxytetracycline, tetracycline, chlortetracycline, minocycline, methacycline, demeclocycline and doxycycline in egg, fish and shrimp. The procedure consisted of a trichloracetic acid/methanol extraction conducted at elevated temperature (60°C) and pressure (65bar), without further clean-up, the extraction solution was concentrated and finally for high-performance liquid chromatography analysis. The limits of detection were 5.0-10.0μg/kg and the limits of quantification were 10.0-15.0μg/kg for tetracyclines in egg, fish and shrimp using UV detection. The analytical limits CCα and CCβ were also calculated. The recoveries of tetracyclines spiked at levels of 15-300μg/kg, averaged 75.6-103.5% with the relative standard deviation values less than 11%. The optimized procedure has been successfully applied to real samples in our laboratories. It demonstrated that the new method was robust and useful for monitoring and quantification of 7 tetracycline residues in food of animal origin.
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, No. 68 South Xuefu Road, Changqing Garden, Wuhan, Hubei 430023, PR China.
Yu
Y
Liu
Hailan
H
Yang
Sheng
S
Yang
Qiwei
Q
Hu
Hongbo
H
Cheng
Huiyu
H
Liu
Yinsheng
Y
Qiu
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004355:Drug Stability; D004531:Eggs / Q000032:analysis*; D005399:Fishes; D016014:Linear Models; D059625:Liquid-Liquid Extraction / Q000379:methods*; D033561:Penaeidae; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D013696:Temperature; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D013754:Tetracyclines