Lactobacillus rhamnosus GG antagonizes Giardia intestinalis induced oxidative stress and intestinal disaccharidases: an experimental study.
The present study describes the in vivo modulatory potential of Lactobacillus rhamnosus GG (LGG), an effective probiotic, in Giardia intestinalis-infected BALB/c mice. Experimentally, it was observed that oral administration of lactobacilli prior or simultaneous with Giardia trophozoites to mice, efficiently (p < 0.05) reduced both the severity and duration of giardiasis. More specifically, probiotics fed, Giardia-infected mice, showed a significant increase in the levels of antioxidants [reduced glutathione (GSH) and superoxide dismutase (SOD)] and intestinal disaccharidases [sucrase and lactase] and decreased levels of oxidants in the small intestine, in comparison with Giardia-infected mice. Histopathological findings also revealed almost normal cellular morphology of the small intestine in probiotic-fed Giardia-infected mice compared with fused enterocytes, villous atrophy and increased infiltration of lymphocytes in Giardia-infected mice. The results of the present study has shed new light on the anti-oxidative properties of LGG in Giardia mediated tissue injury, thereby suggesting that the effects of probiotic LGG are biologically plausible and could be used as an alternative microbial interference therapy.
Department of Microbiology, Panjab University, Chandigarh 160014, India.
Nisha
N
Goyal
Praveen
P
Rishi
Geeta
G
Shukla
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004186:Disaccharidases / Q000378:metabolism*; D004195:Disease Models, Animal; D016829:Giardia lamblia / Q000254:growth & development* / Q000472:pathogenicity*; D005873:Giardiasis / Q000473:pathology* / Q000628:therapy; D006651:Histocytochemistry; D007421:Intestine, Small / Q000737:chemistry / Q000473:pathology; D052201:Lacticaseibacillus rhamnosus / Q000254:growth & development*; D051379:Mice; D018384:Oxidative Stress; D019936:Probiotics / Q000008:administration & dosage*
D000975:Antioxidants; D004186:Disaccharidases