Analysis of tumor-associated mucin glycotopes by Western transfer methods.
In mucins, glycosylation is complex and the most predominant posttranslational modification. Since mucins exhibit differential glycosylation pattern under physiological and pathological conditions, analysis of mucin glycans is important for understanding their specific functions during pathological conditions like cancer. Given the complexity of mucin glycans, several sophisticated analytical tools such as HPLC, mass spectrometry, and lectin sandwich assays are employed for glyco-analysis of mucins. However the specialized expertise and instrumentation required for such analysis are beyond the reach of an average cancer biology laboratory. We described in this chapter the utility of the simple electrophoresis/immunoblotting method to examine the mucin glycan epitopes, using specific antibodies and lectins.
Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Vinayaga S
VS
Gnanapragassam
Maneesh
M
Jain
Surinder K
SK
Batra
D015153:Blotting, Western / Q000379:methods*; D045744:Cell Line, Tumor; D006031:Glycosylation; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D055264:Mucin-4 / Q000378:metabolism; D009077:Mucins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*
D055264:Mucin-4; D009077:Mucins; D011134:Polysaccharides