Overexpression of ANXA1 in penile carcinomas positive for high-risk HPVs.
The incidence of penile cancer varies between populations but is rare in developed nations. Penile cancer is associated with a number of established risk factors and associated diseases including phimosis with chronic inflammation, human papillomavirus (HPV) infection, poor hygiene and smoking. The objective of this study was to identify genes related to this type of cancer. The detection of HPV was analyzed in 47 penile squamous cell carcinoma samples. HPV DNA was detected in 48.9% of penile squamous cell carcinoma cases. High-risk HPV were present in 42.5% of cases and low-risk HPV were detected in 10.6% of penile squamous cell carcinomas. The RaSH approach identified differential expression of Annexin A1 (ANXA1), p16, RPL6, PBEF1 and KIAA1033 in high-risk HPV positive penile carcinoma; ANXA1 and p16 were overexpressed in penile squamous cells positive for high-risk HPVs compared to normal penile samples by qPCR. ANXA1 and p16 proteins were significantly more expressed in the cells from high-risk HPV-positive penile carcinoma as compared to HPV-negative tumors (p<0.0001) independently of the subtype of the carcinoma. Overexpression of ANXA1 might be mediated by HPV E6 in penile squamous cell carcinoma of patients with high-risk HPVs, suggesting that this gene plays an important role in penile cancer.
São Paulo State University, São José do Rio Preto, São Paulo, Brazil.
Marilia Freitas
MF
Calmon
Mânlio Tasso de Oliveira
MT
Mota
Érica
É
Babeto
Natália Maria
NM
Candido
Ana Paula
AP
Girol
Carlos Fabian
CF
Mendiburu
Jane Lopes
JL
Bonilha
Rodrigo Vellasco Duarte
RV
Silvestre
Bruno Miziara
BM
Rosa
Jorge Alberto
JA
Thomé
Gustavo Hernandez Américo
GH
Medeiros
Fernando Augusto
FA
Soares
Gustavo Cardoso
GC
Guimarães
José Germano Ferraz
JG
de Arruda
Sonia Maria
SM
Oliani
Luisa Lina
LL
Villa
José
J
Vassallo
Paula
P
Rahal
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017305:Annexin A1 / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000502:physiology*; D030361:Papillomavirus Infections / Q000150:complications* / Q000473:pathology / Q000821:virology; D010412:Penile Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012307:Risk Factors
D017305:Annexin A1