Martelella endophytica sp. nov., an antifungal bacterium associated with a halophyte.
A Gram-staining-negative, non-spore-forming endophytic bacterium, designated strain YC6887(T), was isolated from a root sample of a halophyte, Rosa rugosa, collected from a tidal flat area of Namhae Island, located at the southern end of Korea. Strain YC6887(T) was found to exhibit inhibitory activity against oomycete plant pathogens. The cells were non-motile and aerobic rods. The strain was able to grow at 4-40 °C (optimum 28-30 °C) and at pH 5.0-9.0 (optimum pH 7.0-8.5). Strain YC6887(T) was able to grow at NaCl concentrations of 0-9 % (w/v) with optimum growth at 4-5 % (w/v) NaCl, but NaCl is not essential for growth. Comparison of 16S rRNA gene sequences showed that the strain was a member of the genus Martelella, a member of order Rhizobiales, exhibiting highest similarity with Martelella mediterranea (98.6 %). The DNA-DNA relatedness between strain YC6887(T) and M. mediterranea MACL11(T) was 19.8 ± 6.8. Chemotaxonomically, strain YC6887(T) contained C19 : 0 cyclo ω8c (28.0 %) and C18 : 1ω7c (17.9 %) as predominant fatty acids, confirming the affiliation of strain YC6887(T) with the genus Martelella. The major respiratory quinone was Q-10 and the DNA G+C content was 62.1 mol%. On the basis of phylogenetic analysis, physiological and biochemical characterization and DNA-DNA hybridization data, strain YC6887(T) should be classified as representing a novel species of the genus Martelella, for which the name Martelella endophytica sp. nov. is proposed. The type strain is YC6887(T) ( = KCCM 43011(T) = NBRC 109149(T)).
King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.|Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Fehmida
F
Bibi
Department of Research & Development, JGreen Inc., Jinju 660-841, Korea.
Eu Jin
EJ
Chung
Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Ajmal
A
Khan
Department of Life Sciences, Chung-Ang University, Seoul 156-756, Korea.
Che Ok
CO
Jeon
Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Young Ryun
YR
Chung
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D027826:Rosa / Q000382:microbiology*; D055051:Salt-Tolerant Plants / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10