Highly fluorescent peptide nanoribbon impregnated with Sn-porphyrin as a potent DNA sensor.
Highly fluorescent and thermo-stable peptide nanoribbons (PNRs) were fabricated by solvothermal self-assembly of a single peptide (D,D-diphenyl alanine peptides) with Sn-porphyrin (trans-dihydroxo[5,10,15,20-tetrakis(p-tolyl)porphyrinato] Sn(IV) (SnTTP(OH)2)). The structural characterization of the as-prepared nanoribbons was performed by transmitting electron microscopy (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM), FT-IR and Raman spectroscopy, indicating that the lipophilic Sn-porphyrins are impregnated into the porous surface formed in the process of nanoribbon formation through intermolecular hydrogen bonding of the peptide main chains. Consequently the Sn-porphyrin-impregnated peptide nanoribbons (Sn-porphyrin-PNRs) exhibited typical UV-visible absorption spectrum of the monomer porphyrin with a red shifted Q-band, and their fluorescence quantum yield was observed to be enhanced compared to that of free Sn-porphyrin. Interestingly the fluorescence intensity and lifetimes of Sn-porphyrin-PNRs were selectively affected upon interaction with nucleotide base sequences of DNA while those of free Sn-porphyrins were not affected by binding with any of the DNA studied, indicating that DNA-induced changes in the fluorescence properties of Sn-porphyrin-PNRs are due to interaction between DNA and the PNR scaffold. These results imply that Sn-porphyrin-PNR will be useful as a potent fluorescent protein analogue and as a biocompatible DNA sensor.
Molecular/Nano Photochemistry and Photonics Laboratory, Department of Chemistry, Chungnam National University, Daehak-ro, Yuseong-gu, Daejeon, 305-764, Republic of Korea.
Sreenivasan Koliyat
SK
Parayil
Jooran
J
Lee
Minjoong
M
Yoon
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D006860:Hydrogen Bonding; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014001:Tin / Q000737:chemistry*
D037742:Nanotubes, Carbon; D010455:Peptides; D011166:Porphyrins; D014001:Tin; D004247:DNA