Sod1 deficiency reduces incubation time in mouse models of prion disease.
Prion infections, causing neurodegenerative conditions such as Creutzfeldt-Jakob disease and kuru in humans, scrapie in sheep and BSE in cattle are characterised by prolonged and variable incubation periods that are faithfully reproduced in mouse models. Incubation time is partly determined by genetic factors including polymorphisms in the prion protein gene. Quantitative trait loci studies in mice and human genome-wide association studies have confirmed that multiple genes are involved. Candidate gene approaches have also been used and identified App, Il1-r1 and Sod1 as affecting incubation times. In this study we looked for an association between App, Il1-r1 and Sod1 representative SNPs and prion disease incubation time in the Northport heterogeneous stock of mice inoculated with the Chandler/RML prion strain. No association was seen with App, however, significant associations were seen with Il1-r1 (P = 0.02) and Sod1 (P<0.0001) suggesting that polymorphisms at these loci contribute to the natural variation observed in incubation time. Furthermore, following challenge with Chandler/RML, ME7 and MRC2 prion strains, Sod1 deficient mice showed highly significant reductions in incubation time of 20, 13 and 24%, respectively. No differences were detected in Sod1 expression or activity. Our data confirm the protective role of endogenous Sod1 in prion disease.
Medical Research Council Prion Unit and Department of Neurodegenerative Disease, University College London Institute of Neurology, London, United Kingdom.
Shaheen
S
Akhtar
Julia
J
Grizenkova
Adam
A
Wenborn
Holger
H
Hummerich
Mar
M
Fernandez de Marco
Sebastian
S
Brandner
John
J
Collinge
Sarah E
SE
Lloyd
D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D004195:Disease Models, Animal; D056726:Genetic Association Studies; D006801:Humans; D051379:Mice; D020641:Polymorphism, Single Nucleotide; D017096:Prion Diseases / Q000235:genetics* / Q000503:physiopathology; D040641:Quantitative Trait Loci / Q000235:genetics; D053702:Receptors, Interleukin-11 / Q000235:genetics; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics*; D000072105:Superoxide Dismutase-1
C545487:APP protein, human; D016564:Amyloid beta-Protein Precursor; D053702:Receptors, Interleukin-11; C000606290:SOD1 protein, human; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1