Genes expressed in cotton  (Gossypium hirsutum) buds isolated with a subtractive library.
A  subtractive cDNA library from cotton buds was constructed to prospect for  differentially expressed genes related to early bud development. A library was  constructed and 768 cDNA sequences were obtained, comprising 168 clusters, with  126 contigs and 42 singlets. Both the Gossypium as well as Arabidopsis databases were utilized for the in silico analysis, since some genes  identified in cotton have not yet been studied for functionality, although they  have homology with genes from other species. The transcriptome revealed a large  number of transcripts, some of them with unknown function, and others related  to pollen development, pollen tubes, ovules, and fibers at different stages.  The most populated contig was identified as fiber from 0-10 days after  anthesis, with 12 reads. The success and novelty rates generated from the  library were 67 and 51%, respectively. The information obtained here will  provide a framework for research on functional cotton genomics.
Departamento de Pós-Graduação em Biotecnologia, Rede Nordeste de Biotecnologia, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
M P N
MP
Pinheiro
V G L
VG
Batista
N F
NF
Martins
R C
RC
Santos
P A
PA
Melo Filho
C R C
CR
Silva
L M
LM
Lima
D032561:Cotton Fiber; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development*; D059467:Transcriptome / Q000235:genetics
D018076:DNA, Complementary