The effects of splenectomy on lipid peroxidation and neuronal loss in experimental spinal cord ischemia/reperfusion injury.
OBJECTIVE
The aim of this study is to investigate the effects of prior splenectomy on     oxidative stress and neuronal loss following spinal cord ischemia and     reperfusion injury.


METHODS
Twenty-one Sprague Dawley rats were     randomly divided into three groups as sham laparatomy (n=7), spinal cord     ischemia/reperfusion (SCIR) injury (n=7) and splenectomy+spinal cord     ischemia/reperfusion (SSCIR) injury (n=7). In the latter group, splenectomy was     performed 3 days before the SCIR injury. The activity of lipid peroxidation in     the spinal cord was assessed by malondialdehyde (MDA), superoxide dismutase     (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. The     difference between the 3 groups was compared using Kruskal-Wallis test. The     histological differences were assessed by counting the viable neurons.


RESULTS
SCIR injury resulted in a decrease of tissue lipid peroxidation activities.     There was statistically significance between the three groups (p < 0.05), but     there was not any significance between the SCIR and SSCIR injury groups (p >     0.05). The number of viable neurons was higher in SSCIR group when comparing     with SCIR group (p < 0.05).


CONCLUSIONS
SCIR injury affects lipid     peroxidation in rats. Prior splenectomy does not attenuate lipid peroxidation,     but prevents neuronal loss after SCIR injury.
Gulhane Military Medical Academy, Department of Neurosurgery, Ankara, Turkey.
Caglar
C
Temiz
Ilker
I
Solmaz
Ozkan
O
Tehli
Serdar
S
Kaya
Onder
O
Onguru
Erhan
E
Arslan
Yusuf
Y
Izci
D000818:Animals; D002374:Catalase / Q000378:metabolism; D016923:Cell Death / Q000502:physiology; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008315:Malondialdehyde / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology*; D013116:Spinal Cord / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D020760:Spinal Cord Ischemia / Q000378:metabolism* / Q000473:pathology*; D013156:Splenectomy; D013482:Superoxide Dismutase / Q000378:metabolism
D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase