Analysis of Mogroside V in Siraitia grosvenorii with micelle-mediated cloud-point extraction.
BACKGROUND
Mogroside V is the main effective ingredient of Siraitia grosvenorii used as a natural sweet food as well as a traditional Chinese medicine. The sample pre-treatment prior to chromatographic analysis requires large amounts of toxic organic solvents and is time consuming.


OBJECTIVE
To develop an effective and simple method for extracting and determining mogroside V of Siraitia grosvenorii.


METHODS
Mogroside V was extracted and preconcentrated by micelle-mediated cloud-point extraction with nonionic surfactant isotridecyl poly (ethylene glycol) ether (Genapol® X-080). The obtained solutions containing mogroside V were analysed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The chromatographic separation was performed on a C18 -column using gradient elution with acetonitrile and water at 203 nm.


RESULTS
The cloud-point extraction yield was 80.7% while the pre-concentration factor was about 10.8. The limit of detection was 0.75 µg/mL and the limit of quantification was 2 µg/mL. The relative standard deviations for intra- and interday precisions of mogroside V were less than 8.68% and 5.78%, respectively, and the recoveries were between 85.1% and 103.6%.


CONCLUSIONS
The HPLC-UV method based on micelle-mediated cloud-point extraction for determination mogroside V in Siraitia grosvenorii was environmentally friendly, simple and sensitive.
Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Fuli
F
Deng
Xiangfeng
X
Liang
Liangrong
L
Yang
Qingfen
Q
Liu
Huizhou
H
Liu
D000097:Acetonitriles / Q000737:chemistry; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019662:Cucurbitaceae / Q000737:chemistry*; D057230:Limit of Detection; D008516:Medicine, Chinese Traditional; D008823:Micelles; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis*; D014466:Ultraviolet Rays
D000097:Acetonitriles; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D014315:Triterpenes; C041740:mogroside V; C032159:acetonitrile