A molecular beacon and graphene oxide-based fluorescent biosensor for Cu(2+) detection.
In this work, we report a "turn-on" fluorescent strategy for the direct detection of Cu(2+) in solutions using molecular beacons (MBs) and graphene oxide (GO). MBs are special single-stranded DNA and carry fluorescence sources. GO is a new nanomaterial having remarkable physical properties. In the sensing system, GO was used as an efficient fluorescence quencher upon the adsorption of MBs, which reduced the background signal and made the detection method highly sensitive. In the presence of Cu(2+), the MBs were cut into short pieces and released by the GO, leading to fluorescence restoration. The detection limit of the sensing strategy was ∼50nM, which is sufficiently sensitive for practical applications. The sensing method also exhibited high selectivity in testing samples containing other metal ions. The application of the method for drinking water is demonstrated.
Department of Mechanical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Jiahao
J
Huang
Qingbin
Q
Zheng
Jang-Kyo
JK
Kim
Zhigang
Z
Li
D015374:Biosensing Techniques / Q000295:instrumentation*; D003300:Copper / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D010087:Oxides / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D015342:DNA Probes; D010087:Oxides; D006108:Graphite; D003300:Copper