A novel mosquito-borne Orbivirus species found in South-east Asia.
The genus Orbivirus of the family Reoviridae includes a genetically diverse group of dsRNA arthropod-borne viruses that infect a wide variety of animal species. Here, we report the complete genome and phylogenetic analysis of a novel orbivirus (IAn-66411 or Sathuvachari virus, SVIV) isolated in 1963 from starlings (Brahminy myna) collected in Vellore, Tamil Nadu, India. Comparative genetic analysis of the SVIV polymerase (VP1 protein), core protein (VP3) and outer core protein (VP7) confirmed that SVIV is most closely related to the mosquito-borne orbiviruses, but that it is equally divergent from all known species. Therefore, SVIV should be tentatively considered as the prototype of a novel mosquito-associated Orbivirus species. These findings will aid in the development of molecular reagents that can identify genetically similar orbiviruses and help elucidate their geographical distribution, epidemiology, species tropism and possible disease association.
Center for Infection and Immunity, Columbia University, New York, NY 10032, USA.
Amit
A
Kapoor
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
Robert B
RB
Tesh
Center for Infection and Immunity, Columbia University, New York, NY 10032, USA.
Raja
R
Duraisamy
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
Vsevolod L
VL
Popov
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
Amelia P A
APA
Travassos da Rosa
Center for Infection and Immunity, Columbia University, New York, NY 10032, USA.
W Ian
WI
Lipkin
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001715:Bird Diseases / Q000635:transmission / Q000821:virology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D009033:Culicidae / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics; D007194:India; D007303:Insect Vectors / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012088:Reoviridae Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D046749:Starlings / Q000821:virology*; D014709:Vero Cells; D014758:Viral Core Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics
D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D012324:RNA-Dependent RNA Polymerase