[Mild oxidative stress in fruit fly Drosophila melanogaster caused by products of sucrose hydrolysis].
The influence of 6% sucrose and equimolar mixture of glucose and fructose in larva diet on the level of oxidized proteins and lipids as well as the activity of antioxidant and associated enzymes in adult fruit fly Drosophila melanogaster was investigated. Larva growing on the diet with sucrose led to the mild oxidative stress development in adult insects, which was differently expressed in both sexes. In males mainly molecules of proteins were subjected to oxidative damages, whereas in females--lipid molecules. This is evidenced by 77% increased content of protein carbonyl groups and decreased (by 40%) level of protein SH-groups in males fed on sucrose. In females fed on sucrose the content of lipid peroxides was by 44% higher, than in individuals, hold on the diet with equimolar mixture of glucose and fructose. The oxidative stress in females was accompanied with increased activity of catalase, superoxide dismutase and thiredoxin reductase by 30, 15 and 34%, respectively. The obtained results suggest that uptake mode of glucose and fructose affects free radical processes in fruit flies.
B M
BM
Rovenko
O V
OV
Lushchak
O V
OV
Lozins'kyĭ
O I
OI
Kubrak
V I
VI
Lushchak
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004032:Diet; D004331:Drosophila melanogaster / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005632:Fructose / Q000378:metabolism* / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation; D012737:Sex Factors; D013395:Sucrose / Q000378:metabolism* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism
D019476:Insect Proteins; D008054:Lipid Peroxides; D005632:Fructose; D013395:Sucrose; D002374:Catalase; D013482:Superoxide Dismutase; D013880:Thioredoxin-Disulfide Reductase; D005947:Glucose