Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping.
We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu City 30010, Taiwan.
Hsu-Hua
HH
Yeh
Shu-Lin
SL
Chang
Yi-Ming
YM
Chiang
Kenneth S
KS
Bruno
Berl R
BR
Oakley
Tung-Kung
TK
Wu
Clay C C
CC
Wang
D000447:Aldehydes / Q000737:chemistry; D001233:Aspergillus nidulans / Q000201:enzymology*; D002264:Carboxylic Acids / Q000737:chemistry; D015394:Molecular Structure; D048630:Polyketide Synthases / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods
D000447:Aldehydes; D002264:Carboxylic Acids; D048630:Polyketide Synthases