Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers.
In the silkworm, Bombyx mori, normal markings are mainly controlled by the +P gene, which is located on the second chromosome. Due to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the +P gene based on an SSR linkage map using silkworm strains P50 and H9, which are normal marking and sex-limited marking, respectively. The +P gene was found to be linked to 3 SSR markers. Using a reciprocal BC1M cross, we constructed a linkage map of 22.5 cM, with +P mapped at 11.3 cM and the nearest SSR marker S0206 at a distance of 3.0 cM. Based on a fine genome map of domesticated silkworms, Kaikoblast analysis showed that the physical distance between the nearest markers (containing the +P gene) is 995 kb. Further analysis showed that BGIBMGA009689, BGIBMGA009688, and BGIBMGA009687 are closer to +P, and that BGIBMGA009689 is closest to +P, with a physical distance of 19.1 kb.
School of Life Science, Anhui Agricultural University, Hefei, Anhui Province, China.
G Q
GQ
Wei
L
L
Yu
C L
CL
Liu
B J
BJ
Zhu
H J
HJ
Ding
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D059006:Chromosomes, Insect / Q000235:genetics; D017344:Genes, Insect; D008040:Genetic Linkage; D005819:Genetic Markers; D007178:Inbreeding; D018895:Microsatellite Repeats
D005819:Genetic Markers