Amino acid building blocks for Fmoc solid-phase synthesis of peptides phosphocholinated at serine, threonine, and tyrosine.
Phosphocholination of eukaryotic host cell proteins has recently been identified as a novel post-translational modification important for bacterial pathogenesis. Here, we describe the first straightforward synthetic strategy for peptides containing phosphocholinated serine, threonine, or tyrosine residues using preformed functional amino acid building blocks, fully compatible with standard Fmoc solid-phase peptide synthesis.
Department of Chemical Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn Strasse 11, D-44227 Dortmund, Germany.
Michael F
MF
Albers
Christian
C
Hedberg
D000596:Amino Acids / Q000737:chemistry*; D005449:Fluorenes / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D010767:Phosphorylcholine / Q000138:chemical synthesis* / Q000737:chemistry*; D012694:Serine / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques; D013912:Threonine / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry*
C496789:9-fluorenylmethoxycarbonyl; D000596:Amino Acids; D005449:Fluorenes; D010455:Peptides; D010767:Phosphorylcholine; D013912:Threonine; D014443:Tyrosine; D012694:Serine