AFM-based force spectroscopy on polystyrene brushes: effect of brush thickness on protein adsorption.
Herein we present a study on nonspecific binding of proteins at highly dense packed hydrophobic polystyrene brushes. In this context, an atomic force microscopy tip was functionalized with concanavalin A to perform single-molecule force spectroscopy measurements on polystyrene brushes with thicknesses of 10 and 60 nm, respectively. Polystyrene brushes with thickness of 10 nm show an almost two times stronger protein adsorption than brushes with a thickness of 60 nm: 72 pN for the thinner and 38 pN for the thicker layer, which is in qualitative agreement with protein adsorption studies conducted macroscopically by fluorescence microscopy.
Physikalisches Institut, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Straße 10, 48149 Münster, Germany.
Carsten
C
Hentschel
Hendrik
H
Wagner
Jens
J
Smiatek
Andreas
A
Heuer
Harald
H
Fuchs
Xi
X
Zhang
Armido
A
Studer
Lifeng
L
Chi
D000327:Adsorption; D055767:Immobilized Proteins / Q000737:chemistry*; D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011137:Polystyrenes / Q000737:chemistry*; D013816:Thermodynamics
D055767:Immobilized Proteins; D011137:Polystyrenes