The cellular protein TIP47 restricts Respirovirus multiplication leading to decreased virus particle production.
The cellular tail-interacting 47-kDa protein (TIP47) acts positively on HIV-1 and vaccinia virus production. We show here that TIP47, in contrast, acts as a restriction factor for Sendai virus production. This conclusion is supported by the occurrence of increased or decreased virus production upon its suppression or overexpression, respectively. Pulse-chase metabolic labeling of viral proteins under conditions of TIP47 suppression reveals an increased rate of viral protein synthesis followed by increased incorporation of viral proteins into virus particles. TIP47 is here described for the first time as a viral restriction factor that acts by limiting viral protein synthesis.
Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Carole
C
Bampi
Anne-Sophie Gosselin
AS
Grenet
Grégory
G
Caignard
Pierre-Olivier
PO
Vidalain
Laurent
L
Roux
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D015497:HIV-1; D006801:Humans; D000070796:Perilipin-3; D011506:Proteins; D029082:Sendai virus / Q000276:immunology* / Q000502:physiology*; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D019562:Viral Load; D014771:Virion; D014779:Virus Replication
C038196:PLIN3 protein, human; D000070796:Perilipin-3; D011506:Proteins; D033921:Vesicular Transport Proteins