Deficiency in spliceosome-associated factor CTNNBL1 does not affect ongoing cell cycling but delays exit from quiescence and results in embryonic lethality in mice.
CTNNBL1 is an armadillo-repeat protein that associates with the CDC5L/Prp19 complex of the spliceosome. Unlike the majority of spliceosomal proteins (and despite having no obvious homologs), CTNNBL1 is inessential for cell viability as revealed by studies in both vertebrate B cell lines and in fission yeast. Here, however, we show that ablation of CTNNBL1 in the mouse germline results in mid-gestation embryonic lethality but that lineage-specific CTNNBL1 ablation in early B cell precursors does not affect the production and abundance of mature B lymphocytes. However, CTNNBL1-deficient resting B lymphocytes show sluggish exit from quiescence on cell activation, although once entry into cycle has initiated, proliferation and differentiation in response to mitogenic stimuli continue largely unaffected. A similar sluggish exit from quiescence is also observed on reprovision of nutrients to nitrogen-starved CTNNBL1-deficient yeast. The results indicate that, whereas other RNA splicing-associated factors have been connected to cell cycle progression, CTNNBL1 plays no essential role in cycling cells but does fulfill an evolutionarily conserved function in helping cells to undergo efficient exit from quiescence following activation.
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Anita
A
Chandra
Febe
F
van Maldegem
Simon
S
Andrews
Michael S
MS
Neuberger
Cristina
C
Rada
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000172:deficiency* / Q000378:metabolism; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D020964:Embryo Loss / Q000235:genetics / Q000473:pathology*; D018390:Gene Targeting; D017781:Genes, Immediate-Early / Q000235:genetics; D018095:Germ-Line Mutation / Q000235:genetics; D017578:Immunoglobulin Class Switching / Q000187:drug effects / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009687:Nuclear Proteins / Q000172:deficiency* / Q000378:metabolism; D012326:RNA Splicing / Q000187:drug effects / Q000235:genetics; D016196:S Phase / Q000187:drug effects; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D017381:Spliceosomes / Q000187:drug effects / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; C551916:CTNNBL1 protein, mouse; D008070:Lipopolysaccharides; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins
B lymphocyte; CTNNBL1; RNA splicing; cell cycle; conditional knockout mice; quiescence; yeast