Bacterial small RNA-based negative regulation: Hfq and its accomplices.
A large group of bacterial small regulatory RNAs (sRNAs) use the Hfq chaperone to mediate pairing with and regulation of mRNAs. Recent findings help to clarify how Hfq acts and highlight the role of the endonuclease RNase E and its associated proteins (the degradosome) in negative regulation by these sRNAs. sRNAs frequently uncouple transcription and translation by blocking ribosome access to the mRNA, allowing other proteins access to the mRNA. As more examples of sRNA-mediated regulation are studied, more variations on how Hfq, RNase E, and other proteins collaborate to bring about sRNA-based regulation are being found.
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Nicholas
N
De Lay
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Daniel J
DJ
Schu
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892. Electronic address: gottesms@helix.nih.gov.
Susan
S
Gottesman
D004722:Endoribonucleases / Q000378:metabolism / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D035001:Host Factor 1 Protein / Q000502:physiology*; D009097:Multienzyme Complexes / Q000378:metabolism / Q000502:physiology; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism / Q000502:physiology; D020365:RNA Helicases / Q000378:metabolism / Q000502:physiology; D020871:RNA Stability; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000502:physiology*; D019779:Salmonella enterica / Q000201:enzymology / Q000235:genetics*
D029968:Escherichia coli Proteins; C087373:Hfq protein, E coli; D035001:Host Factor 1 Protein; D009097:Multienzyme Complexes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D058727:RNA, Small Untranslated; C111244:degradosome; D011117:Polyribonucleotide Nucleotidyltransferase; D004722:Endoribonucleases; D020365:RNA Helicases