Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification.
Toxic mushroom species, such as the death cap ( Amanita phalloides ), are responsible for most mushroom poisonings. In the present work, novel loop-mediated isothermal amplification (LAMP) assays were used for the differentiation of even closely related edible and toxic mushroom species. The applicability of these methods was tested by cross-reaction studies and analysis of spiked mushroom samples (raw and fried material). Contaminations at the level of 2% (w/w) could be detected in different mushroom blends. Three detection methods were used: agarose gel analysis, fluorimetric real-time detection, and visual detection by lateral flow dipsticks (LFD). The LAMP assay combined with LFD detection allows the identification of  A. phalloides  in about 2 h (including DNA extraction) at a very low level of technical equipment (micropestle, water bath, and mobile centrifuge), which makes this technique perfectly suited for on-site applications.
Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Hamburg, Germany.
Franziska
F
Vaagt
Ilka
I
Haase
Markus
M
Fischer
D000363:Agaricales / Q000145:classification* / Q000235:genetics*; D016533:Mycological Typing Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D014675:Vegetables / Q000145:classification* / Q000235:genetics*