Antimycin A effect on the electron transport in chloroplasts of two Chlamydomonas reinhardtii strains.
The effects of antimycin A on the redox state of plastoquinone and on electron donation to photosystem I (PS I) were studied in sulfur-deprived Chlamydomonas reinhardtii cells of the strains cc406 and 137c. We found that this reagent suppresses cyclic electron flow around PS I in the cc406 strain, whereas this inhibitory effect was completely absent in the 137c strain. In the latter strain, antimycin A induced rapid reduction of plastoquinone in the dark and considerably enhanced the rate of electron donation to P700 (+) in the dark. Importantly, neither myxothiazol, an inhibitor of mitochondrial respiration, FCCP, a protonophore, nor propyl gallate, an inhibitor of the plastid terminal oxidase, induced such a strong effect like antimycin A. The results indicate that in the chloroplast of the 137c strain, antimycin A has a site of action outside of the machinery of cyclic electron flow.
Biological Faculty, Moscow State University, Vorobyevi Gory, 119992, Moscow, Russia. taras_an@mail.ru
Taras K
TK
Antal
Galina P
GP
Kukarskikh
Alexander A
AA
Bulychev
Esa
E
Tyystjärvi
Tatyana
T
Krendeleva
D000968:Antimycin A / Q000494:pharmacology*; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects* / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects*; D008954:Models, Biological
D000968:Antimycin A