Equilibrious strand exchange promoted by DNA conformational switching.
Most of DNA strand exchange reactions in vitro are based on toehold strategy which is generally nonequilibrium, and intracellular strand exchange mediated by proteins shows little sequence specificity. Herein, a new strand exchange promoted by equilibrious DNA conformational switching is verified. Duplexes containing c-myc sequence which is potentially converted into G-quadruplex are designed in this strategy. The dynamic equilibrium between duplex and G4-DNA is response to the specific exchange of homologous single-stranded DNA (ssDNA). The SER is enzyme free and sequence specific. No ATP is needed and the displaced ssDNAs are identical to the homologous ssDNAs. The SER products and exchange kenetics are analyzed by PAGE and the RecA mediated SER is performed as the contrast. This SER is a new feature of G4-DNAs and a novel strategy to utilize the dynamic equilibrium of DNA conformations.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, P R of China.
Zhiguo
Z
Wu
Xiao
X
Xie
Puzhen
P
Li
Jiayi
J
Zhao
Lili
L
Huang
Xiang
X
Zhou
D000255:Adenosine Triphosphate / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D054856:G-Quadruplexes; D007700:Kinetics; D009690:Nucleic Acid Conformation; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D044366:Transition Temperature
D004277:DNA, Single-Stranded; D016271:Proto-Oncogene Proteins c-myc; D000255:Adenosine Triphosphate; D004247:DNA