Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast             cancer cell lines.
The ErbB-2 gene, whose overexpression is observed in many types of tumors             including breast cancer, plays an important role in carcinoma formation. Dysregulation             of the human transforming acidic coiled-coil 1 (TACC1) and ErbB-2 genes is thought             to be important in the development and progression of breast cancer. However,             a putative interaction between ErbB-2 and TACC1 remains undetermined in breast             cancer. After infecting BT474 cells with lentiviral-mediated ErbB2-specific shRNA,             we detected the expression of ErbB-2 and TACC1 by real-time PCR and western blotting.             ErbB-2 mRNA expression was decreased in the Lenti-ShERBB2 infected cells, and             western blotting indicated a concordant reduction in ErbB-2 protein. TACC1 expression             at the mRNA and protein levels was significantly upregulated by ErbB-2 silencing             in BT474 cells. CCK-8 assay indicated that the inhibition of ErbB-2 expression             increased the sensitivity of BT474 cells to docetaxel treatment. These findings             provide proof and the foundation for the molecular and biological relationships             of ErbB-2 and TACC1 in breast cancer.
Department of Oncology, The Affiliated Hospital of the Medical College, Qingdao University, Qingdao 266003, PR China.
Jinyu
J
Xiang
Wensheng
W
Qiu
Xingang
X
Wang
Fang
F
Zhou
Zhen
Z
Wang
Shihai
S
Liu
Lu
L
Yue
D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D000077143:Docetaxel; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D005326:Fetal Proteins / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D043823:Taxoids / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D000970:Antineoplastic Agents; D000077143:Docetaxel; D005326:Fetal Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D043823:Taxoids; C120584:TACC1 protein, human