Determination of light-medium-heavy polycyclic aromatic hydrocarbons in vegetable oils by solid-phase extraction and high-performance liquid chromatography with diode array and fluorescence detection.
A new method for determination of 16 polycyclic aromatic hydrocarbons (PAHs)-naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benz[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenz[a,h]anthracene, benzo[g,h,i]perylene, and indeno[1,2,3-cd]pyrene-in vegetable oils was developed. Solid-phase extraction (SPE) prior to high-performance liquid chromatography with fluorescence detection could be used for all those PAHs except acenaphthylene. Acenaphthylene could be detected using a diode array detector at 228 nm. The parameters and variables that affect the extraction were investigated. Under optimum conditions: the extract reagent was centrifuged at 4 °C and evaporated. After that a SPE procedure was used for further cleanup. The limits of detection and limits of quantification  were in the range of 0.01-2.35 and 0.04-7.00 μg kg(-1) in vegetable oil, respectively. The relative standard deviations were under 5%.
Pony Lab for Physical & Chemical Analysis, Pony Test International Group, Shanghai, China. wjzhao119@yahoo.com.cn
Wei-jun
WJ
Zhao
Xiang-bin
XB
Chen
Ling
L
Fang
Chun-lei
CL
Li
Dao-yuan
DY
Zhao
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D010938:Plant Oils / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
D010938:Plant Oils; D011084:Polycyclic Aromatic Hydrocarbons