Gamete fertility and ovule number variation in selfed reciprocal F1 hybrid triploid plants are heritable and display epigenetic parent-of-origin effects.
Polyploidy and hybridization play major roles in plant evolution and reproduction. To investigate the reproductive effects of polyploidy and hybridization in Arabidopsis thaliana, we analyzed fertility of reciprocal pairs of F1 hybrid triploids, generated by reciprocally crossing 89 diploid accessions to a tetraploid Ler-0 line. All F1 hybrid triploid genotypes exhibited dramatically reduced ovule fertility, while variation in ovule number per silique was observed across different F1 triploid genotypes. These two reproductive traits were negatively correlated suggesting a trade-off between increased ovule number and ovule fertility. Furthermore, the ovule fertility of the F1 hybrid triploids displayed both hybrid dysgenesis and hybrid advantage (heterosis) effects. Strikingly, both reproductive traits (ovule fertility, ovule number) displayed epigenetic parent-of-origin effects between genetically identical reciprocal F1 hybrid triploid pairs. In some F1 triploid genotypes, the maternal genome excess F1 hybrid triploid was more fertile, whilst for other accessions the paternal genome excess F1 hybrid triploid was more fertile. Male gametogenesis was not significantly disrupted in F1 triploids. Fertility variation in the F1 triploid A. thaliana is mainly the result of disrupted ovule development. Overall, we demonstrate that in F1 triploid plants both ovule fertility and ovule number are subject to parent-of-origin effects that are genome dosage-dependent.
Plant and AgriBiosciences Centre (PABC), School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Dorota
D
Duszynska
Plant and AgriBiosciences Centre (PABC), School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Peter C
PC
McKeown
Section of Integrative Biology & Institute for Cellular and Molecular Biology, University of Texas, 1 University Station C0930, Austin, TX, USA.
Thomas E
TE
Juenger
Plant and AgriBiosciences Centre (PABC), School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.|Molecular Cytology, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Science Park 904, 1098XH, Amsterdam, the Netherlands.
Anna
A
Pietraszewska-Bogiel
Department of Plant Production, Faculty of Bioscience Engineering, University of Ghent, 9000, Ghent, Belgium.
Danny
D
Geelen
Plant and AgriBiosciences Centre (PABC), School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Charles
C
Spillane
D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D004171:Diploidy; D005298:Fertility; D005838:Genotype; D006823:Hybrid Vigor / Q000235:genetics; D006824:Hybridization, Genetic; D040582:Inheritance Patterns / Q000235:genetics*; D056771:Ovule / Q000235:genetics* / Q000502:physiology; D011058:Pollen / Q000235:genetics; D011123:Polyploidy; D012639:Seeds / Q000235:genetics; D057945:Self-Fertilization / Q000235:genetics*