Synthesis of biotinylated c-di-gmp and c-di-amp using click conjugation.
The biotinylated c-di-GMP and c-di-AMP conjugates 10a/b were synthesized by a straightforward set of procedures from standard, commercially available phosphoramidites. Their availability should allow isolation and characterization of new protein and RNA receptors for these key bacterial signaling molecules.
Department of Chemistry and Chemical Biology Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
Barbara L
BL
Gaffney
Nicholas D
ND
Stebbins
Roger A
RA
Jones
D019921:Biotinylation; D011232:Chemical Precipitation; D057930:Click Chemistry; D003460:Crystallization; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry
D015226:Dinucleoside Phosphates; D009943:Organophosphorus Compounds; C528998:cyclic diadenosine phosphate; C434331:phosphoramidite; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP