Long-range PCR based sequencing of the highly homologous genes, SFTPA1 and SFTPA2.
In humans, Surfactant Protein A exists as two highly homologous genetic isoforms, SFTPA1 and SFTPA2. Mutations in these two genes are associated with idiopathic pulmonary fibrosis (IPF) and lung cancer. We have developed a Sanger DNA sequencing assay which utilizes long-range PCR to detect mutations in these two genes.
Children's Medical Center, 1935 Medical District Dr., Dallas, TX 75235, United States. christopher.horton@childrens.com
Christopher J
CJ
Horton
Midori
M
Mitui
Nora K
NK
Leos
Christine Kim
CK
Garcia
Jason Y
JY
Park
D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics*; D017422:Sequence Analysis, DNA
D037662:Pulmonary Surfactant-Associated Protein A; C445842:SFTPA1 protein, human; C445843:SFTPA2 protein, human