Intra and inter-user reliability of central corneal thickness measurements obtained in healthy feline eyes using a portable spectral-domain optical coherence tomography device.
OBJECTIVE
To determine the intra and inter-user reliability of central corneal thickness (CCT) measurements obtained using a portable spectral-domain optical coherence tomography (SD-OCT) device in healthy feline eyes.


METHODS
Twenty clinically normal cats.


METHODS
Gentle manual restraint was utilized for proper animal positioning. Central corneal thickness measurements were obtained from both eyes of each animal three times by two operators in succession. A 6 mm, corneal pachymetry protocol was performed using the Optovue® iVue SD-OCT system. The CCT was automatically calculated using the pachymetry software generated from eight radial scans, 6 mm in length. The CCT was recorded for each operator and compared statistically for both intra and inter-user reliability.


RESULTS
Twenty cats (11 CM, 9 SF) with a mean age of 4.7 years were examined. The mean CCT for all eyes examined was 629.08 ± 47.05 μm. No significant difference in CCT was found based on the eye examined (OD vs. OS) or age of the animal. However, the CCT of spayed females was significantly higher (42.64 ± 18.0 μm) as compared to castrated males (P = 0.03). There was no significant difference in CCT measurements between replicates performed by the same operator or between operators. The mean difference in CCT between operators was 0.38 μm. The coefficient of variation for each user and between users was very low (range 0.68-1.5%). The intra-class correlation coefficient comparing operators was 0.975.


CONCLUSIONS
The Optovue® iVue SD-OCT device is capable of obtaining CCT measurements with excellent intra and interoperator reliability in healthy feline eyes.
Department of Clinical Sciences, Tufts Cummings School of Veterinary Medicine, North Grafton, MA, 01536, USA.
Anthony F
AF
Alario
Christopher G
CG
Pirie
D000818:Animals; D002415:Cats; D003315:Cornea / Q000033:anatomy & histology*; D005260:Female; D008297:Male; D015588:Observer Variation; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*
central corneal thickness; feline; optical coherence tomography; pachymetry