Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein.
Leukocyte cell-derived chemotaxin 2 (LECT2) is a secreted hepatic protein that has been associated with several physiological activities. LECT2 belongs to the peptidase M23 family, suggesting that it is a zinc-binding protein. To test this possibility, electrospray ionization mass spectrometry and X-ray absorption fine-structure analysis were performed. Results of these experiments indicated that recombinant mouse LECT2 produced by an animal cell line contains a zinc atom. Furthermore, the recombinant LECT2 was found to be self-oligomerized by disulfide bonds in vitro, but this was suppressed by addition of zinc. These results indicated that zinc stabilizes the LECT2 structure.
Department of Diabetic Complications, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Akinori
A
Okumura
Takehiro
T
Suzuki
Hideyuki
H
Miyatake
Tomoya
T
Okabe
Yuki
Y
Hashimoto
Takuya
T
Miyakawa
Hai
H
Zheng
Hiroyuki
H
Unoki-Kubota
Hideaki
H
Ohno
Naoshi
N
Dohmae
Yasushi
Y
Kaburagi
Yoshitsugu
Y
Miyazaki
Masaru
M
Tanokura
Satoshi
S
Yamagoe
D000682:Amyloid / Q000737:chemistry; D000818:Animals; D016466:CHO Cells; D002614:Chelating Agents / Q000737:chemistry; D006224:Cricetinae; D003553:Cystine / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D004795:Enzyme Stability; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry*; D008248:Lysostaphin / Q000737:chemistry; D045726:Metalloproteases / Q000737:chemistry; D051379:Mice; D008970:Molecular Weight; D011485:Protein Binding; D055503:Protein Multimerization; D021241:Spectrometry, Mass, Electrospray Ionization; D015032:Zinc / Q000737:chemistry*
D000682:Amyloid; D002614:Chelating Agents; D036341:Intercellular Signaling Peptides and Proteins; C491213:Lect2 protein, mouse; D003553:Cystine; D004492:Edetic Acid; D045726:Metalloproteases; D008248:Lysostaphin; D015032:Zinc