Hydrocortisone inhibits IFN-γ production in equine, ovine, and bovine PBMCs.
Hydrocortisone is widely accepted as an anti-inflammatory agent and there are many products available containing hydrocortisone as an active ingredient. Surprisingly, there is little data available specifically on the immunological effects of hydrocortisone in large animals. Glucocorticoids are well-characterized for their ability to repress inflammation via a wide variety of mechanisms including suppression of cytokine production. In this study the effects of hydrocortisone on IFN-γ production by equine, bovine, and ovine PBMCs were assessed using flow cytometric or ELISpot analysis. Hydrocortisone suppressed mitogen-driven IFN-γ production by PBMCs from all three species of animals, confirming that this agent mediates anti-inflammatory effects in large animals. Although the results from this study were expected based on the precedence set in murine and human systems, it is important to understand the effects of administration of a compound or product in the species of interest as species-specific indications are not always available.
Veterinary Medicine Research and Development, Pfizer Animal Health, Kalamazoo, MI, United States. erin.mccandless@pfizer.com
Erin E
EE
McCandless
Sharath K
SK
Rai
Duncan
D
Mwangi
Laurel
L
Sly
Lilian C
LC
Franz
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006736:Horses; D006854:Hydrocortisone / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D012756:Sheep; D013045:Species Specificity; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000893:Anti-Inflammatory Agents; D007371:Interferon-gamma; D006854:Hydrocortisone