Correlation between expressions of ERCC1/TS mRNA and effects of gastric cancer to chemotherapy in the short term.
OBJECTIVE
To study the correlation between expression levels of ERCC1/TS mRNA and the susceptibility of preoperative chemotherapy for patients with gastric cancer.


METHODS
A total of forty cases with advanced gastric cancer of T3-4N1-2M0 were treated with preoperative chemotherapy according to FLEEOX regimen based on endarterial-intravenous coadministration. Sufficient, fresh gastric tissue specimens were obtained with the help of gastroscope, and the expression levels of ERCC1/TS mRNA were detected by qRT-PCR before chemotherapy. The chemotherapeutic response was evaluated with Choi Criteria after chemotherapy, and pathologic remission extent was observed after surgery. The correlation between the expression levels of ERCC1/TS mRNA before chemotherapy and the chemotherapeutic effect based on imageology and pathology was analyzed.


RESULTS
The response rate of Chemotherapy in this cohort was 80.0 % based on imageology and 51.43 % based on pathology. The expression levels of ERCC1/TS mRNA were significantly associated with imageology remission extent (P = 0.033, P = 0.025) and pathologic remission extent (P = 0.044, P = 0.016), respectively. The chemotherapeutic effect on patients with low-expression levels of ERCC1/TS mRNA was better.


CONCLUSIONS
From the perspective of pathology and imageology evaluating the preoperative chemotherapeutic response for patients with gastric cancer, ERCC1 and TS were used as the molecular predictors and provided prognostic information in this study.
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002 Jiangsu, China.
Liqi
L
Chen
Guoli
G
Li
Jieshou
J
Li
Chaogang
C
Fan
Jian
J
Xu
Bo
B
Wu
Kun
K
Liu
Caihua
C
Zhang
D000368:Aged; D004268:DNA-Binding Proteins / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D013940:Thymidylate Synthase / Q000235:genetics*; D014057:Tomography, X-Ray Computed
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D013940:Thymidylate Synthase; C071447:ERCC1 protein, human; D004720:Endonucleases