Biotransformation of vulgarin.
Using a standard two-stage fermentation technique, the fungus Beauveria bassiana (ATCC 7159) was found to convert the eudesmanolide vulgarin (1) to 1α,4α-dihydroxy-5αH,6,11βH-eudesman-6,12-olide (2). The use of the yeastHansenula anomala ATCC 20170 instead, produced the less polar 4α-hydroxy-1-oxo-5αH,6,11βH-eudesman-6,12-olide(3), in addition to the more polar 3α,4α-dihydroxy-1-oxo-5αH,6,11βH-eudesman-6,12-olide (4). These metabolites were characterized on the basis of their spectral data and the identity of 4 was further confirmed by chemical synthesis.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Health Sciences Center, Kuwait University, Safat 13110, Kuwait. kyorabi@hsc.edu.kw
Khaled Y
KY
Orabi
Farouk S
FS
El-Feraly
Waleed A
WA
Al-Sulmy
Mohammed A
MA
Al-Yahya
D052982:Beauveria / Q000378:metabolism; D019149:Bioreactors; D001711:Biotransformation; D010843:Pichia / Q000378:metabolism; D012717:Sesquiterpenes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
D012717:Sesquiterpenes; C474733:vulgarin (eudesmane)