[HPLC fingerprint of Liuwei Dihuang condensed pills].
OBJECTIVE
To establish HPLC fingerprints of Liuwei Dihuang condensed pills.


METHODS
Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile (containing 0.05% phosphoric) -water (containing 0.05% phosphoric) as the mobile phase. The column temperature was set at 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was 276 nm (0-10 min), 236 nm (10-40 min) and 276 nm (40-60 min). The sample size was 20 microL. Chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method.


RESULTS
Good precision, stability and repeatability were proved. Q-TOF-MS-IDA-MS/ MS method was adopted for qualitative determination of eighteen chromatographic peaks. Ten batches of Liuwei Dihuang condensed pills were determined with the method, and their similarities were above 0. 96.


CONCLUSIONS
The study lays a foundation for the overall quality evaluation of Liuwei Dihuang condensed pills.
Key Laboratory of Structure Based Drug Design & Discovery of Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. gaoxinzhu653808@163.com
Xin-Biao
XB
Gao
Lei
L
Sun
Shan-Yi
SY
Qiao
Song
S
Gao
Yan-Zhong
YZ
Che
Ke-Rong
KR
Zhang
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D011786:Quality Control; D013607:Tablets / Q000032:analysis
D004365:Drugs, Chinese Herbal; C042579:Liuwei Dihuang Decoction; D013607:Tablets