Evaluation of the expression of p53, MDM2, and SUMO-1 in oral lichen planus.
OBJECTIVE
The objective of this study was to compare the expression of proteins p53, MDM2, and SUMO-1 in oral lichen planus (OLP) lesions, epithelial dysplasia, and squamous cell carcinoma.


METHODS
The sample consisted of the following five groups of cheek mucosa lesions: normal mucosa (NM), inflammatory fibrous hyperplasia (IFH), lichen planus, epithelial dysplasia, and squamous cell carcinoma. The tissue samples were stained with hematoxylin-eosin and submitted to immunohistochemistry using anti-p53, anti-MDM2, and anti-SUMO-1 antibodies.


RESULTS
The results of this study demonstrated similar expression of p53 and MDM2 between OLP, oral epithelial dysplasia and, to a lesser extent, between OLP and oral squamous cell carcinoma (OSCC). However, for SUMO-1 a similar expression was observed in OLP, NM, and IFH.


CONCLUSIONS
The results demonstrated overexpression of important proteins (p53 and MDM2) related to regulatory mechanisms of apoptosis in OLP, suggesting that there is a favorable environment for malignant transformation. The expression of SUMO-1 in OLP was similar to NM and IFH, suggesting that alterations of this protein occur at later stages of carcinogenesis, because important overexpression occurred in oral epithelial dysplasia and OSCC.
Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, UNESP - Univ Estadual Paulista, São José dos Campos, São Paulo, Brazil.
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M
Oliveira Alves
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I
Balducci
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Y
Rodarte Carvalho
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Cabral
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F
Nunes
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J
Almeida
D005786:Gene Expression Regulation; D016158:Genes, p53 / Q000502:physiology*; D006801:Humans; D017676:Lichen Planus, Oral / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D025842:SUMO-1 Protein / Q000235:genetics*
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2
SUMO-1 protein; carcinoma; immunohistochemical; lichen planus; proto-oncogene proteins c-mdm2; squamous cell; tumor suppressor protein p53