Brain-friendly amperometric enzyme biosensor based on encapsulated oxygen generating biomaterial.
A novel first-generation Clark-type biosensor platform that can eliminate the oxygen dependence has been presented. Sufficient oxygen to drive the enzymatic reaction under hypoxic conditions was produced by encapsulated oxygen generating biomaterial, calcium peroxide. The catalase immobilized in chitosan matrix was coated on top of the groove to decompose residual hydrogen peroxide to oxygen. A glucose biosensor was developed on the proposed platform as proof of concept. Under hypoxic conditions, developed glucose biosensors maintained their sensitivity response around 84% of their response at oxygen tension of 151 mmHg. The sensitivity deviation was less than 5.3% with the oxygen tension traversed from 0 to 57 mmHg. Under oxygen tension of 8.3 mmHg, the sensitivity of 37.130 nA/mM and the linear coefficient of R(2)=0.9968 were obtained with the glucose concentration varying from 0.05 to 10mM. This new platform is particularly attractive for injured brain monitoring.
Cushing Neuromonitoroing Laboratory at Feinstein Institute for Medical Research, Manhasset, NY 11030, USA. cli11@nshs.edu
Chunyan
C
Li
Zhizhen
Z
Wu
Jed A
JA
Hartings
Neena
N
Rajan
Nadeen
N
Chahine
Cletus
C
Cheyuo
Ping
P
Wang
Pei-Ming
PM
Wu
Eugene V
EV
Golanov
Chong H
CH
Ahn
Raj K
RK
Narayan
D001672:Biocompatible Materials; D015374:Biosensing Techniques; D001921:Brain / Q000378:metabolism*; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D008839:Microelectrodes; D010100:Oxygen / Q000378:metabolism*
D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D010100:Oxygen