Cordycepin (3'-deoxyadenosine) pentostatin (deoxycoformycin) combination treatment of mice experimentally infected with Trypanosoma evansi.
The aim of this study was to evaluate the anti-trypanosomal effect of treatment with 3'-deoxyadenosine (cordycepin) combined with deoxycoformycin (pentostatin: inhibitor of the enzyme adenosine deaminase) in vitro by using mice experimentally infected with Trypanosoma evansi. In vitro, a dose-dependent trypanocidal effect of cordycepin was observed against the parasite. In the in vivo trials, the two drugs were used individually and in combination of different doses. The drugs when used individually had no curative effect on infected mice. However, the combination of cordycepin (2 mg kg-1) and pentostatin (2 mg kg-1) was 100% effective in the T. evansi-infected groups. There was an increase in levels of some biochemical parameters, especially on liver enzymes, which were accompanied by histological lesions in the liver and kidneys. Based on these results we conclude that treatment using the combination of 3'-deoxyadenosine with deoxycoformycin has a curative effect on mice infected with T. evansi. However, the therapeutic protocol tested led to liver and kidney damage, manifested by hepatotoxicity and nephrotoxicity.
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil.
Luciana
L
Dalla Rosa
Aleksandro S
AS
da Silva
Lucas T
LT
Gressler
Camila B
CB
Oliveira
Maria G C
MG
Dambrós
Luiz C
LC
Miletti
Raqueli T
RT
França
Sonia T A
ST
Lopes
Yasmin N
YN
Samara
Marcelo L
ML
da Veiga
Silvia G
SG
Monteiro
D000818:Animals; D003839:Deoxyadenosines / Q000008:administration & dosage / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination / Q000662:veterinary; D005260:Female; D051379:Mice; D015649:Pentostatin / Q000008:administration & dosage / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D014345:Trypanosoma / Q000145:classification* / Q000187:drug effects; D014352:Trypanosomiasis / Q000188:drug therapy*
D003839:Deoxyadenosines; D014344:Trypanocidal Agents; D015649:Pentostatin; C058120:cordycepin