Genetically encoded Ca(2+) indicators: properties and evaluation.
Genetically encoded calcium ion (Ca(2+)) indicators have become very useful and widely used tools for Ca(2+) imaging, not only in cellular models, but also in living organisms. However, the in vivo and in situ characterization of these indicators is tedious and time consuming, and it does not provide information regarding the suitability of an indicator for particular experimental environments. Thus, initial in vitro evaluation of these tools is typically performed to determine their properties. In this review, we examined the properties of dynamic range, affinity, selectivity, and kinetics for Ca(2+) indicators. Commonly used strategies for evaluating these properties are presented. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.
Department of Biomolecular Science and Engineering, Osaka University, Ibaraki, Osaka, Japan.
Vadim
V
Pérez Koldenkova
Takeharu
T
Nagai
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D006801:Humans; D007202:Indicators and Reagents / Q000032:analysis / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D002118:Calcium