Establishment and characterization of a novel lymphangiosarcoma cell line (MO-LAS) compared with the hemangiosarcoma cell line (ISO-HAS).
The concept of "lymphangiosarcoma" remains obscure. Therefore, we reported a patient with lymphangiosarcoma, resistant to immunotherapy. The patient presented with impressive and discriminative features: clinically an ill-defined edematous lesion with lymphorrhea and pathologically atypical vascular channel formation without extravasation of blood, clearly distinguished from common angiosarcoma with hemorrhage. From this case, a lymphangiosarcoma cell line, MO-LAS, was established and its characteristics were compared with the hemangiosarcoma cell line, ISO-HAS. Flow cytometric analysis revealed that MO-LAS was negative for factor VIII-related antigen, but positive for CD31, D2-40, NZ-1, and vascular endothelial growth factor receptor-3 (VEGFR-3), similar to ISO-HAS. However, MO-LAS expressed a much higher level of homeobox gene PROX1, indicating a lymphatic phenotype, compared with ISO-HAS. Furthermore, MO-LAS showed a much lesser expression of oncogenes and much lower sensitivity against lymphokine-activated killer (LAK) cells. Lymphangiosarcoma may be difficult to recognize by the immune system. Conclusively, the establishment of MO-LAS, a novel angiosarcoma cell line bearing lymphatic characters, strongly suggests the entity of lymphangiosarcoma.
Department of Regulation Biochemistry, Kitasato University School of Allied Sciences Sagamihara, Japan. mikio@med.kitasato-u.ac.jp
Mikio
M
Masuzawa
Mamiko
M
Masuzawa
Yuhko
Y
Hamada
Nobuko
N
Arakawa
Mari
M
Mori
Masako
M
Ishii
Shigeo
S
Nishiyama
D000368:Aged; D001706:Biopsy; D002273:Carcinogens; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D015870:Gene Expression; D006394:Hemangiosarcoma / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D008204:Lymphangiosarcoma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010641:Phenotype; D012867:Skin / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D000098551:Prospero-Related Homeobox 1 Protein
D002273:Carcinogens; D018398:Homeodomain Proteins; D025521:Tumor Suppressor Proteins; D000098551:Prospero-Related Homeobox 1 Protein
Cell line; PROX1; hemangiosarcoma; lymphangiosarcoma