A Rat Basophilic Leukaemia cell sensor for the detection of pathogenic viruses.
A Rat Basophilic Leukemia (RBL) cell sensor is developed for the detection and identification of pathogenic viruses. Recombinant sdAb-Fc antibodies were constructed by linking virus-specific single domain antibody to mouse IgE-Fc fragment. The sdAb-Fc can bind to FcεRI receptors on RBL cells and can be cross-linked by target viruses leading to cell activation and Ca(2+) influx reflected by the increase of intracellular fluorescence. The responses of RBL cells to viruses in real time could be observed using fluorescence microscopy. 10(3) TCID50 of H5N1 viruses and 10 LD50 of rabies viruses could be detected in less than three minutes. An excess quantity of non-relevant viruses did not interfere with the recognition of target viruses.
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Mingsheng
M
Qu
Bhargavi M
BM
Boruah
Wei
W
Zhang
Yan
Y
Li
Wenjun
W
Liu
Yuhai
Y
Bi
George F
GF
Gao
Ruifu
R
Yang
Dawei
D
Liu
Bin
B
Gao
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification*; D015471:Leukemia, Basophilic, Acute / Q000821:virology*; D011820:Rabies virus / Q000302:isolation & purification*; D051381:Rats; D019562:Viral Load / Q000295:instrumentation*