Insulin induces neurite outgrowth via SIRT1 in SH-SY5Y cells.
Insulin plays diverse roles, including learning and memory, in the central nervous system. SIRT1 has been reported to be involved in the processes of normal learning, memory, and synaptic plasticity. However, whether insulin is directly involved in regulating SIRT1 expression in neurons or whether it affects synapses remains largely unknown. Here, we show that insulin promotes neurite outgrowth and increases SIRT1 expression in SH-SY5Y cells. LY294002, a phosphatidylinositol 3-kinase inhibitor, inhibited the expression of insulin-induced increases in SIRT1. Conversely, the downregulation of SIRT1 using a SIRT1 inhibitor and SIRT1-siRNA resulted in a significant reduction in the length of neurite outgrowth. Taken together, these results suggest that the regulation of SIRT1 by insulin is important for the neurite outgrowth of neuroblastoma cells.
Institute of Laboratory Animal Science, Chinese Academy of Medical Science (CAMS) & Comparative Medical Center, Peking Union Medical College (PUMC), Beijing, China.
Y
Y
Liu
Z
Z
Yao
L
L
Zhang
H
H
Zhu
W
W
Deng
C
C
Qin
D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002867:Chromones / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D009025:Morpholines / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D056564:Sirtuin 1 / Q000378:metabolism*; D013569:Synapses / Q000187:drug effects / Q000378:metabolism
D002867:Chromones; D004791:Enzyme Inhibitors; D007328:Insulin; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D056564:Sirtuin 1; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C447939:SIRT1 protein, human