Characterization of nucleolin K88 acetylation defines a new pool of nucleolin colocalizing with pre-mRNA splicing factors.
Nucleolin is a multifunctional protein that carries several post-translational modifications. We characterized nucleolin acetylation and developed antibodies specific to nucleolin K88 acetylation. Using this antibody we show that nucleolin is acetylated in vivo and is not localized in the nucleoli, but instead is distributed throughout the nucleoplasm. Immunofluorescence studies indicate that acetylated nucleolin is co-localized with the splicing factor SC35 and partially with Y12. Acetylated nucleolin is expressed in all tested proliferating cell types. Our findings show that acetylation defines a new pool of nucleolin which support a role for nucleolin in the regulation of mRNA maturation and transcription by RNA polymerase II.
Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS USR 3010, Laboratoire Joliot-Curie, Lyon, France.
Sadhan
S
Das
Rong
R
Cong
Jayasha
J
Shandilya
Parijat
P
Senapati
Benoit
B
Moindrot
Karine
K
Monier
Hélène
H
Delage
Fabien
F
Mongelard
Sanjeev
S
Kumar
Tapas K
TK
Kundu
Philippe
P
Bouvet
D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002466:Cell Nucleolus / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008239:Lysine / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism*; D000097796:Nucleolin
D007106:Immune Sera; D008239:Lysine; D010446:Peptide Fragments; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D017411:Ribonucleoproteins, Small Nuclear; D000097796:Nucleolin