Expression of DJ-1 proteins in placentas from women with severe preeclampsia.
OBJECTIVE
Lately, DJ-1 has been identified as a novel mediator of hypoxia-induced cellular responses. The aim of this study was to determine the difference of DJ-1 expression in the placentas of women with normal and preeclamptic pregnancies.


METHODS
DJ-1 mRNA expression was quantified by quantitative reverse transcription polymerase chain reaction (RT-PCR). Also, immunoperoxidase and immunofluorescent staining for DJ-1 was performed on 11 normal and 12 preeclamptic placental tissues.


RESULTS
The expression of mRNA of DJ-1 was significantly higher in the study group than in the control group. The immunoreactivity was especially higher in the syncytiotrophoblast of preeclamptic placentas compared to the control group.


CONCLUSIONS
Overexpression of the DJ-1 protein in the placentas of severe PE patients is thought to be a causative or compensatory mechanism in response to hypoxia, and this finding might contribute to a better understanding of the pathophysiology of preeclampsia.
Department of Obstetrics and Gynecology, Konkuk University School of Medicine, Seoul, Republic of Korea.
Han-Sung
HS
Kwon
Han-Sung
HS
Hwang
In-Sook
IS
Sohn
Seung-Hwa
SH
Park
D000328:Adult; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D015513:Oncogene Proteins / Q000096:biosynthesis*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D000071617:Protein Deglycase DJ-1; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D012333:RNA, Messenger; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1