Genotoxicity of dried Hoodia parviflora aerial parts.
Hoodia parviflora is being developed commercially for use in weight loss food and dietary supplement products. Its effects are ascribed to a number of glycosides that have been shown to be present in plant extracts from several Hoodia species, the best known of which is H. gordonii. H. parviflora has been identified as an alternative to H. gordonii, and, as part of the process to develop H. parviflora, in vitro genotoxicity tests, as recommended by recent European Food Safety Authority guidance, were conducted on a dried powder preparation of H. parviflora aerial parts. The preparation was tested for reverse mutation at doses up to 5,000μg/plate in Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537, and in Escherichia coli WP2 uvrA TA, both in the presence and in the absence of an exogenous source of metabolic activation (rat liver S9). In addition, the dried powder was evaluated in an in vitro cytotoxicity chromosome aberration assay using human lymphocytes. Test conditions included both a 4 (up to 2500μg/mg) and 44-h exposure period (up to 1000μg/mg) and the incorporation of an exogenous source of metabolic activation (4-h exposure only). H. parviflora dried powder was non-genotoxic in both in vitro assays.
Intertek Cantox, Suite 308, 2233 Argentia Rd., Mississauga, Ontario, Canada L5N 2X7. barry.lynch@intertek.com
Barry
B
Lynch
Annette
A
Lau
Nigel
N
Baldwin
Hana
H
Hofman-Hüther
Mark R
MR
Bauter
Palma Ann
PA
Marone
D000818:Animals; D029062:Apocynaceae / Q000737:chemistry*; D001711:Biotransformation; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D018514:Plant Structures; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics
D009153:Mutagens