Effects of various drugs (flunixin, pentoxifylline, enoxaparin) modulating micro-rheological changes in cerulein-induced acute pancreatitis in the rat.
Previously we have investigated the cerulein-induced acute pancreatitis and provided data on its micro-rheological impact in the rat. We hypothesized that non-steroid anti-inflammatory agent flunixin, the xanthine-derivate pentoxifylline and the low molecular weight heparin enoxaparin may have various beneficial effects improving microcirculatory and rheological parameters. In female rats, under general anesthesia, 10 μg/kg cerulein s.c. was administered and 2 hours afterwards microcirculation was tested by laser Doppler flowmetry on the tongue and after performing laparotomy on the small intestine, liver and pancreas prior to terminal blood sampling. From blood samples hematological parameters, blood pH, lactate concentration, erythrocyte deformability, osmoscan parameters and erythrocyte aggregation were tested. Compared to normal control in acute pancreatitis group we found severe deterioration in tissue microcirculation together with impaired erythrocyte deformability and enhanced aggregation, accompanied by acidic pH and increasing lactate concentration. Improvement was found when using flunixin (s.c.), pentoxifylline (i.p.) or enoxaparin (s.c.). These drugs could partly improve the blood flux on the surface of the investigated organs, and the flunixin had the most expressed improving effects on micro-rheological parameters. Surprisingly, the improving effect of pentoxifylline on micro-rheological parameters was not obvious (red blood cell deformability did not improved better than in the other treated groups), however, microcirculatory parameters improved.
Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Zsolt
Z
Szentkereszty
Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Robert
R
Kotan
Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Ferenc
F
Kiss
Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Zoltan
Z
Klarik
Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Janos
J
Posan
Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Istvan
I
Furka
Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Peter
P
Sapy
Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Iren
I
Miko
Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Katalin
K
Peto
Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Norbert
N
Nemeth
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D000925:Anticoagulants / Q000494:pharmacology / Q000627:therapeutic use*; D002108:Ceruletide; D003002:Clonixin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D017984:Enoxaparin / Q000494:pharmacology / Q000627:therapeutic use*; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004907:Erythrocyte Deformability / Q000187:drug effects; D005260:Female; D008833:Microcirculation / Q000187:drug effects*; D010195:Pancreatitis / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy*; D010431:Pentoxifylline / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D014665:Vasodilator Agents / Q000494:pharmacology / Q000627:therapeutic use*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000925:Anticoagulants; D017984:Enoxaparin; D014665:Vasodilator Agents; C014557:flunixin; D002108:Ceruletide; D010431:Pentoxifylline; D003002:Clonixin
Red blood cell deformability; cerulein-induced acute pancreatitis; microcirculation; osmoscan; rat model; red blood cell aggregation; various drug therapy