Allopregnanolone elevations following pregnenolone administration are associated with enhanced activation of emotion regulation neurocircuits.
BACKGROUND
The neurosteroid allopregnanolone is a potent allosteric modulator of the gamma-aminobutyric acid type A receptor with anxiolytic properties. Exogenous administration of allopregnanolone reduces anxiety, and allopregnanolone blockade impairs social and affective functioning. However, the neural mechanism whereby allopregnanolone improves mood and reduces anxiety is unknown. In particular, brain imaging has not been used to link neurosteroid effects to emotion regulation neurocircuitry.


METHODS
To investigate the brain basis of allopregnanolone's impact on emotion regulation, participants were administered 400 mg of pregnenolone (n=16) or placebo (n=15) and underwent 3T functional magnetic resonance imaging while performing the shifted-attention emotion appraisal task, which probes emotional processing and regulation.


RESULTS
Compared with placebo, allopregnanolone was associated with reduced activity in the amygdala and insula across all conditions. During the appraisal condition, allopregnanolone increased activity in the dorsal medial prefrontal cortex and enhanced connectivity between the amygdala and dorsal medial prefrontal cortex, an effect that was associated with reduced self-reported anxiety.


CONCLUSIONS
These results demonstrate that in response to emotional stimuli, allopregnanolone reduces activity in regions associated with generation of negative emotion. Furthermore, allopregnanolone may enhance activity in regions linked to regulatory processes. Aberrant activity in these regions has been linked to anxiety psychopathology. These results thus provide initial neuroimaging evidence that allopregnanolone may be a target for pharmacologic intervention in the treatment of anxiety disorders and suggest potential future directions for research into neurosteroid effects on emotion regulation neurocircuitry.
Department of Psychiatry, University of Michigan, Ann Arbor, Michigan 48109, USA. rekaufma@umich.edu
Rebecca K
RK
Sripada
Christine E
CE
Marx
Anthony P
AP
King
Jessica C
JC
Rampton
S Shaun
SS
Ho
Israel
I
Liberzon
D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D001288:Attention / Q000187:drug effects; D001921:Brain / Q000098:blood supply* / Q000187:drug effects*; D001931:Brain Mapping; D016022:Case-Control Studies; D004644:Emotions / Q000187:drug effects*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D008297:Male; D010100:Oxygen / Q000097:blood; D010364:Pattern Recognition, Visual; D010775:Photic Stimulation; D011280:Pregnanolone / Q000097:blood*; D011284:Pregnenolone / Q000008:administration & dosage*; D011930:Reaction Time / Q000187:drug effects; D013997:Time Factors; D055815:Young Adult
D011284:Pregnenolone; D011280:Pregnanolone; D010100:Oxygen