Evaluation of the antioxidant activity of several naturally occurring coumarins and their synthesized analogues by "ferric reducing antioxidant power" assay.
Coumarins have attracted intense interest in recent years because of their diverse pharmacological properties. According to our continuing investigations of biological effects of several coumarins, the structure-antioxidant activity relationships (SARs) of six naturally occurring coumarins and their 16 synthesized analogues were established. For this purpose, the very reliable colorimetric assay (ferric reducing antioxidant power) modified to be used in 96-well microplates was used. This approach, which determines the reducing capacity of tested compounds directly, has previously been used for the determination of SARs of flavonoids, but has not been used for SAR determination of coumarins. It is known that the biological properties and consequently, therapeutic application of simple coumarins depends upon the pattern of substitution. It was established that 7,8-dihydroxy- and 6,7-dihydroxy-4-methylcoumarins have shown excellent ferric-reducing properties.
Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy .
Zuzana
Z
Rehakova
Vit
V
Koleckar
Ludek
L
Jahodar
Lubomir
L
Opletal
Katerina
K
Macakova
Lucie
L
Cahlikova
Daniel
D
Jun
Kamil
K
Kuca
D000975:Antioxidants / Q000494:pharmacology*; D003374:Coumarins / Q000494:pharmacology*; D005290:Ferric Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction
D000975:Antioxidants; D003374:Coumarins; D005290:Ferric Compounds