[Effect of HSP70 on apoptotic of cartilage cells in knee osteoarthritis].
OBJECTIVE
To investigate the expression and relationship between HSP70 and caspase-3 in knee osteoarthritis.


METHODS
Forty adult SD rats were divided into experimental group and control group. Thirty rats in experimental group, anterior cruciate ligament transaction (ACLT) was cut off and partial meniscectomy of 1/3 inside incision were performed to reproduce knee osteoarthritis (KOA) model according to Hulth methos, and the other 10 rats was treated with nothing as control group. The rats were sent to the cage and free to move. At 1, 2 and 4 weeks later, the arthritis cartilage of femoral and tibial end were observed through immunohistochemistry staining and light microscope. Meanwhile, Mankin scale system was adopted for histomorphology evaluation.


RESULTS
Changes of KOA such as hyperplastic synovium,erosion on the surface of cartilage and so on were found in experiment group, the expression of HSP70 was augmentation all the time, but the expression of caspase-3 was reduction 1 week later; no similar changes were found in control group. Mankin scale system showed that there were significant differences in the first week as compared with the second week and 4th week (both P < 0.01).


CONCLUSIONS
Heat shock protein inhibit the apoptosis of cartilage cells and protect the cartilage cells in knee osteoarthritis, the conservative treatment for clinical provide objective scientific basis.
Department of Rehabilitation, the 3rd Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, China. ovemightjo@msn.com
Shou-Yu
SY
Xu
Xin-Miao
XM
Yao
Yun
Y
Zhai
Wen-Sheng
WS
Pan
Zhen
Z
Fang
Bang-Jian
BJ
He
Jing-Hong
JH
Xu
D000818:Animals; D017209:Apoptosis; D002356:Cartilage / Q000473:pathology*; D053148:Caspase 3 / Q000502:physiology; D019902:Chondrocytes / Q000473:pathology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D008297:Male; D020370:Osteoarthritis, Knee / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018840:HSP70 Heat-Shock Proteins; D053148:Caspase 3