Inflammation subverts hippocampal synaptic plasticity in experimental multiple sclerosis.
Abnormal use-dependent synaptic plasticity is universally accepted as the main physiological correlate of memory deficits in neurodegenerative disorders. It is unclear whether synaptic plasticity deficits take place during neuroinflammatory diseases, such as multiple sclerosis (MS) and its mouse model, experimental autoimmune encephalomyelitis (EAE). In EAE mice, we found significant alterations of synaptic plasticity rules in the hippocampus. When compared to control mice, in fact, hippocampal long-term potentiation (LTP) induction was favored over long-term depression (LTD) in EAE, as shown by a significant rightward shift in the frequency-synaptic response function. Notably, LTP induction was also enhanced in hippocampal slices from control mice following interleukin-1β (IL-1β) perfusion, and both EAE and IL-1β inhibited GABAergic spontaneous inhibitory postsynaptic currents (sIPSC) without affecting glutamatergic transmission and AMPA/NMDA ratio. EAE was also associated with selective loss of GABAergic interneurons and with reduced gamma-frequency oscillations in the CA1 region of the hippocampus. Finally, we provided evidence that microglial activation in the EAE hippocampus was associated with IL-1β expression, and hippocampal slices from control mice incubated with activated microglia displayed alterations of GABAergic transmission similar to those seen in EAE brains, through a mechanism dependent on enhanced IL-1β signaling. These data may yield novel insights into the basis of cognitive deficits in EAE and possibly of MS.
IRCSS Fondazione Santa Lucia, Rome, Italy. robert.nistico@uniroma1.it
Robert
R
Nisticò
Dalila
D
Mango
Georgia
G
Mandolesi
Sonia
S
Piccinin
Nicola
N
Berretta
Marco
M
Pignatelli
Marco
M
Feligioni
Alessandra
A
Musella
Antonietta
A
Gentile
Francesco
F
Mori
Giorgio
G
Bernardi
Ferdinando
F
Nicoletti
Nicola B
NB
Mercuri
Diego
D
Centonze
D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002460:Cell Line; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005260:Female; D059330:GABAergic Neurons / Q000378:metabolism / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D017774:Long-Term Potentiation; D051379:Mice; D009103:Multiple Sclerosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016202:N-Methylaspartate / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000473:pathology; D009435:Synaptic Transmission; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000378:metabolism
D053583:Interleukin-1beta; D016202:N-Methylaspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid