The photosynthetic response of tobacco plants overexpressing ice plant aquaporin McMIPB to a soil water deficit and high vapor pressure deficit.
We investigated the photosynthetic capacity and plant growth of tobacco plants overexpressing ice plant (Mesembryanthemum crystallinum L.) aquaporin McMIPB under (1) a well-watered growth condition, (2) a well-watered and temporal higher vapor pressure deficit (VPD) condition, and (3) a soil water deficit growth condition to investigate the effect of McMIPB on photosynthetic responses under moderate soil and atmospheric humidity and water deficit conditions. Transgenic plants showed a significantly higher photosynthesis rate (by 48 %), higher mesophyll conductance (by 52 %), and enhanced growth under the well-watered growth condition than those of control plants. Decreases in the photosynthesis rate and stomatal conductance from ambient to higher VPD were slightly higher in transgenic plants than those in control plants. When plants were grown under the soil water deficit condition, decreases in the photosynthesis rate and stomatal conductance were less significant in transgenic plants than those in control plants. McMIPB is likely to work as a CO2 transporter, as well as control the regulation of stomata to water deficits.
Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Miki
M
Kawase
Yuko T
YT
Hanba
Maki
M
Katsuhara
D000906:Antibodies; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002245:Carbon Dioxide / Q000378:metabolism*; D035723:Mesembryanthemum / Q000235:genetics*; D058503:Mesophyll Cells / Q000378:metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D054046:Plant Stomata / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018526:Plant Transpiration; D030821:Plants, Genetically Modified; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D012987:Soil; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D055548:Vapor Pressure; D014867:Water / Q000378:metabolism*
D000906:Antibodies; D020346:Aquaporins; D010940:Plant Proteins; D012987:Soil; D014867:Water; D002245:Carbon Dioxide; D012273:Ribulose-Bisphosphate Carboxylase