Selective and quantitative cell detection based both on aptamers and the conventional cell-staining methods.
Aptamer-based biochips for selective cell detection and quantitation in combination of the recent biochip technology and the conventional cell staining methods are described. Using a model system comprising HER2- or PSMA-positive cells as the analytes and single-stranded RNA aptamers specific for HER2 or PSMA as immobilized ligands on chips, we could demonstrate that aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. In particular, our PSMA-specific sensor was found to have the characteristics of good stability, reproducibility and reusability, with detection limit as low as 10(3) LNCaP cells. In conclusion, we could show the suitability of nucleic acid aptamers as low molecular weight receptors on biochips for sensitive and specific cell detection and quantitation for future diagnostics development.
Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
Jisu
J
Kim
Gwan-Ho
GH
Lee
Woong
W
Jung
Sang Soo
SS
Hah
D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D058948:Cell Tracking / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D013194:Staining and Labeling / Q000379:methods*
D052157:Aptamers, Nucleotide; D015415:Biomarkers