[Current methods of cytochrome p450 analysis].
Current review describes recent approaches of cytochrome P450 concentration and activity evaluation. Special attention paid to modem methods of proteomic analysis such as electrophoresis and chromato-mass-spectrometry. Methods of targeted proteomic applicable for quantitative and qualitative study of P450s in biological samples as well as methods for the enzyme activity measurements are reviewed. Finally, data on correlation between certain P450 isoform content and its specific enzymatic activities were described and discussed in the review.
N E
NE
Moskaleva
V G
VG
Zgoda
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008163:Luminescent Measurements; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods
D003577:Cytochrome P-450 Enzyme System