CTLA-4 (+49A/G) polymorphism and type-1 diabetes in Turkish children.
OBJECTIVE
To evaluate the contribution of cytotoxic T-Iymphocyte antigen-4(CTLA-4)+49A/G polymorphism to the susceptibility to type-1 diabetes (T1D) in Turkish children.


METHODS
A case-control study was designed to include 91 Turkish children with T1D and 99 healthy controls. CTLA-4 (+99A/G) gene polymorphism typing was done by PCR amplification followed by restriction fragment length polymorphism method.


RESULTS
The genotype and allele frequencies of the CTLA-4 (+99A/G)polymorphism in patients with T1D were not different from those in the controls (p>0.05). The allele frequency of G was 36.2% in patients with T1D,and 31.8% in controls (p>0.05). Additionally, this polymorphism was not associated with the clinical and laboratory characteristics of the patients with T1D (p>0.05).


CONCLUSIONS
Our case-control study suggests that the CTLA-4 (+99A/G) gene polymorphism is not associated with T1D in the Turkish population.
Antalya Antalya Education and Research Hospital, Department of Pediatric Immunology, Antalya, Turkey.
Fatih
F
Çelmeli
Doğa
D
Türkkahraman
Deniz
D
Özel
Sema
S
Akçurin
Olcay
O
Yegin
D000293:Adolescent; D060908:CTLA-4 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D014421:Turkey
D060908:CTLA-4 Antigen