Buckwheat (Fagopyrum esculentum M.) sprout treated with methyl jasmonate (MeJA) improved anti-adipogenic activity associated with the oxidative stress system in 3T3-L1 adipocytes.
Buckwheat sprouts contain various bioactive compounds including rutin which have a number of biological activities. We have previously shown that buckwheat sprouts (TBWE) treated with methyl jasmonate (MeJA) significantly increased the amount of phenolics and the antioxidant activity. The aim of this study was to demonstrate the effect of TBWE on anti-adipogenesis and pro-oxidant enzyme in 3T3-L1 adipocytes. We also evaluated the anti-oxidative activity of TBWE in adipocytes by using the nitroblue tetrazolium assay. Our data showed that TBWE markedly inhibited adipocyte differentiation and ROS production in 3T3-L1 cells compared with control groups. Moreover, TBWE has strongly shown the inhibition of adipogenic transcription factor as well as pro-oxidant enzymes. Together, we demonstrate that the MeJA treatment significantly increased the amount of phenolic compound, resulting in the suppression of adipogenesis and ROS production in the 3T3-L1 cells. These findings indicate that TBWE has the potential for anti-adipogenesis activity with anti-oxidative properties.
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea. jake@kfri.re.kr.
Young-Jun
YJ
Lee
Kui-Jin
KJ
Kim
Kee-Jai
KJ
Park
Bo-Ra
BR
Yoon
Jeong-Ho
JH
Lim
Ok-Hwan
OH
Lee
D041721:3T3-L1 Cells; D000085:Acetates / Q000494:pharmacology; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D052242:Adiponectin / Q000235:genetics; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D003517:Cyclopentanes / Q000494:pharmacology; D019612:Fagopyrum / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D054883:Oxylipins / Q000494:pharmacology; D047495:PPAR gamma / Q000235:genetics; D010636:Phenols / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics
D000085:Acetates; D052242:Adiponectin; D022763:CCAAT-Enhancer-Binding Protein-alpha; D003517:Cyclopentanes; D054883:Oxylipins; D047495:PPAR gamma; D010636:Phenols; D010936:Plant Extracts; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; C072239:methyl jasmonate; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase