Contribution of microorganisms to non-extractable residue formation during biodegradation of ibuprofen in soil.
Non-extractable residues (NER) formed during biodegradation of organic contaminants in soil are considered to be mainly composed of parent compounds or their primary metabolites with hazardous potential. However, in the case of biodegradable organic compounds, the soil NER may also contain microbial biomass components, for example fatty acids (FA) and amino acids (AA). After cell death, these biomolecules are subsequently incorporated into non-living soil organic matter (SOM) and are stabilised ultimately forming hardly extractable residues of biogenic origin. We investigated biodegradation of (13)C(6)-ibuprofen, in particular the metabolic incorporation of the (13)C-label into FA and AA and their fate in soil over 90 days. (13)C-FA and (13)C-AA amounts in the living microbial biomass fraction initially increased, then decreased over time and were continuously incorporated into the non-living SOM pool. The (13)C-FA in the non-living SOM remained stable from day 59 whereas the contents of (13)C-AA slightly increased until the end. After 90 days, nearly all NER were biogenic as they were made up almost completely by natural biomass compounds. The presented data demonstrated that the potential environmental risks related to the ibuprofen-derived NER are overestimated.
UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Biotechnology, Permoserstrasse 15, 04318 Leipzig, Germany. karolina.nowak@ufz.de
Karolina M
KM
Nowak
Cristobal
C
Girardi
Anja
A
Miltner
Matthias
M
Gehre
Andreas
A
Schäffer
Matthias
M
Kästner
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D005591:Chemical Fractionation; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D007052:Ibuprofen / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
D000596:Amino Acids; D005227:Fatty Acids; D012989:Soil Pollutants; D007052:Ibuprofen