Osteogenic capacity of human BM-MSCs, AT-MSCs and their co-cultures using HUVECs in FBS and PL supplemented media.
Human bone marrow-derived mesenchymal stem cells (BM-MSCs) and human adipose tissue-derived mesenchymal stem cells (AT-MSCs) are the most frequently used stem cells in tissue engineering. Due to major clinical demands, it is necessary to find an optimally safe and efficient way for large-scale expansion of these cells. Considering the nutritional source in the culture medium and method, this study aimed to analyze the effects of FBS- and PL-supplemented media on osteogenesis in stem cell mono- and co-cultures with human umbilical vein endothelial cells (HUVECs). Results showed that cell metabolic activity and proliferation increased in PL- compared to FBS-supplemented media in mono- and co-cultures for both BM-MSCs and AT-MSCs. In addition, calcium deposition was cell type dependent and decreased for BM-MSCs but increased for AT-MSCs in PL-supplemented medium in both mono- and co-cultures. Based on the effects of co-cultures, BM-MSCs/HUVECs enhanced osteogenesis compared to BM-MSCs monocultures in both FBS- and PL-supplemented media whereas AT-MSCs/HUVECs showed similar results compared to AT-MSCs monocultures.
Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands.|Department of Oral and Maxillofacial Surgery, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Jinling
J
Ma
Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands.
Jeroen J J P
JJ
van den Beucken
Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands.
Sanne K
SK
Both
Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, the Netherlands.|Department of Oral and Maxillofacial Surgery, VU University Medical Center / Academic Centre for Dentistry Amsterdam, Amsterdam, the Netherlands.
Henk-Jan
HJ
Prins
Department of Orthopedic Surgery, VU University Medical Center, Amsterdam, the Netherlands.
Marco N
MN
Helder
Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands.
Fang
F
Yang
Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands.
John A
JA
Jansen
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D018920:Coculture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism
D003470:Culture Media
bone tissue engineering; co-culture; fetal bovine serum; osteogenesis; platelet lysate; stem cells