[Induction of VEGF in human monocytes by DENV infection and the regulatory mechanism].
To investigate the effects of DENV infection on the expression of Vascular endothelial growth factor (VEGF) in monocytes, and to explore which innate immune signaling pathway is responsible for VEGF induction. Real-time PCR was used to determine the expression levels of VEGF in DENV-infected THP-1. We found that different serotype viruses (DENV1, DENV2, DENV3) induced the VEGF expression. Moreover, VEGF expression was significantly increased in human primary monocytes infected with DENV 2. In addition, VEGF induction by DENV2 was significantly impaired by knockdown of TLR3 and interferon-beta promoter stimulator 1 (IPS-1), or by inhibition of ERK, JNK or NF-kappaB. These results demonstrated that DENV induced VEGF expression in monocytes, and the activation of TLR3, IPS-1 signal pathways were required for DENV2-triggered VEGF induction, suggesting that VEGF might be a promising therapeutic target for DHF.
Department of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. helishelly@163.com
Li
L
He
Si-Yu
SY
Wu
Ting-Long
TL
Wang
Ping
P
Zhang
Xi
X
Huang
D003715:Dengue / Q000235:genetics* / Q000276:immunology / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020935:MAP Kinase Signaling System; D009000:Monocytes; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000276:immunology
D016328:NF-kappa B; D042461:Vascular Endothelial Growth Factor A