Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis.
Currently in clinic, people use hematoxylin and eosin stain (H&E stain) and immunohistochemistry methods to identify the generation and genre of cancers for human pathological samples. Since these methods are inaccurate and time consuming, developing a rapid and accurate method to detect cancer is urgently demanded. In our study, binding peptides for lung cancer cell line A549 were identified using bacteria surface display method. With those binding peptides for A549 cells on the surface, the fluorescent bacteria (Escherichia coli with stably expressed green fluorescent protein) were served as specific detecting reagents for the diagnosis of cancers. The binding activity of peptide-fluorescent bacteria complex was confirmed by detached cancer cells, attached cancer cells and mice tumor xenograft samples. A unique fixation method was developed for peptide-bacteria complex in order to make this complex more feasible for the clinic use. This peptide-fluorescent bacteria complex has great potential to become a new diagnostic tool for clinical application.
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
Bing
B
Dong
Anxin
A
Wang
Lihua
L
Yuan
Lisha
L
Chen
Kefeng
K
Pu
Wei
W
Duan
Xiyun
X
Yan
Yimin
Y
Zhu
D000818:Animals; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D014183:Transplantation, Heterologous
D005456:Fluorescent Dyes; D010455:Peptides; D049452:Green Fluorescent Proteins