Thermoresponsive substrates used for the expansion of human mesenchymal stem cells and the preservation of immunophenotype.
The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercially available pNIPAm which is both affordable and a significant simplification on alternative approaches used elsewhere. Solvent casting was used to produce films in the micrometer range and successful cell adhesion and proliferation was highly dependent on the thickness of the coating produced with 1 μm thick coatings supporting cells to confluence. 3T3 cell sheets and hMSCs were successfully detached from the cast coatings upon temperature reduction. Furthermore, results indicate that the hMSCs remained undifferentiated as the surface receptor profile of hMSCs was not altered when cells were detached in this manner.
Department of Applied Macromolecular Chemistry, Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006, Madrid, Spain. nash.maria@gmail.com
Maria E
ME
Nash
Xingliang
X
Fan
William M
WM
Carroll
Alexander V
AV
Gorelov
Frank P
FP
Barry
Georgina
G
Shaw
Yury A
YA
Rochev
D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D041681:NIH 3T3 Cells; D013696:Temperature
D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; C052970:poly-N-isopropylacrylamide