[Inhibitory effect of VEGF antisense phosphorothioate oligodeoxynucleotides on the growth of human  salivary adenoid cystic carcinoma xenografts in nude mice].
OBJECTIVE
To investigate the inhibitory effect of VEGF antisense phosphorothioate oligodeoxynucleoiides on the growth of human salivary adenoid cystic carcinoma (SACC) xenografts in nude mice.


METHODS
The VEGF-ASODN was synthesised artificially. After the model of human SACC xenografts in nude mice was established, they were random1y divided into three groups: antisense group, scrambled group and normal saline group. A control group without cancer was also established. Antisense(66 μg), scrambled sequence(66 μg) and normal saline(once every 3 days and 7 times in all) were injected in three experimental groups, respectively. Two days after therapy, the mice were sacrificed. Serums were used for detection of VEGF protein. All tumors were measured and weighted. The quantity of VEGF mRNA and protein and PLI, MVD was detected by hybridization in situ and immunohistochemistry. SPSS13.0 software package was used for statistical analysis.


RESULTS
The VEGF-ASODN could suppress the expression of VEGF in human SACC xenografts in nude mice and reduce VEGF protein in serum of nude mice significantly. It cou1d also reduce the volume and weight of xenografts and could reduce the expression of VEGF mRNA and its protein, PCNA and CD34.


CONCLUSIONS
By inhibiting the expression of VEGF, VEGF-ASODN can inhabit proliferation of human SACC xenografts in nude mice.
Department of Stomatology, Central Hospital of Taian City, Taian 271000, Shandong Province, China. lxg0538@163.com
Xiao-guang
XG
Li
Xu-xia
XX
Wang
Teng-yu
TY
Li
Yan-xiu
YX
Wang
Jing
J
Gao
Chun-xiao
CX
Ni
D000818:Animals; D003528:Carcinoma, Adenoid Cystic; D045744:Cell Line, Tumor; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A