Evaluation of biocompatibility of a new root canal irrigant Q Mix™ 2 in 1- An in vivo study.
OBJECTIVE
To evaluate the biocompatibility of a new root canal irrigant Q mix™ 2 in 1 in comparison to 0.9% sterile saline, 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and 17% ethylenediaminetetraacetic acid (EDTA).


METHODS
Six circles were drawn on the dorsal skin of 24 male Wistar Albino rats, leaving 2cm between each circle. Using a syringe, 0.1mL of each root canal irrigant was injected subcutaneously into five circles. In the sixth circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were done at 2 hours, 48 hours, 14 days, and 30 days postprocedure. Tissue samples were excised, embedded in paraffin blocks, and 3 μm thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated. From each tissue sample, five sections presenting the greatest inflammatory reactions were examined under a light microscope, and analyzed statistically by analysis of variance (ANOVA) and Tukey's test.


RESULTS
At the two-hour examination period, all the irrigants showed a slight increase in the number of inflammatory cells, at 48 hours, the number of inflammatory cells were increased significantly, and after 14 and 30 days, they were decreased gradually. Qmix™ 2 in 1 showed a smaller number of inflammatory cells than other irrigants tested.


CONCLUSIONS
QMix™ 2 in 1 was shown to be less toxic to the rat subcutaneous tissue than 3% NaOCl, 2% CHX, and 17% EDTA.
Department of Conservative Dentistry and Endodontics, Mamata Dental College, Khammam, Andhra Pradesh, India.
Veeramachaneni
V
Chandrasekhar
Vanapatla
V
Amulya
Vanapatla Swaroopa
VS
Rani
T Jaya
TJ
Prakash
A Siva
AS
Ranjani
Ch
Ch
Gayathri
Biocompatibility; root canal irrigant; subcutaneous tissue