Gas-phase synthesis of solid state DNA nanoparticles stabilized by l-leucine.
Aerosol flow reactor is used to generate solid-state nanoparticles in a one-step process that is based on drying of aerosol droplets in continuous flow. We investigated the applicability of aerosol flow reactor method to prepare solid state DNA nanoparticles. Precursor solutions of plasmid DNA with or without complexing agent (polyethylenimine), coating material (l-leucine) and mannitol (bulking material) were dispersed to nanosized droplets and instantly dried in laminar heat flow. Particle morphology, integrity and stability were studied by scanning electron microscopy. The stability of DNA was studied by gel electrophoresis. Plasmid DNA as such degraded in the aerosol flow process. Complexing agent protected DNA from degradation and coating material enabled production of dispersed, non-aggregated, nanoparticles. The resulting nanoparticles were spherical and their mean diameter ranged from 65 to 125nm. The nanoparticles were structurally stable at room temperature and their DNA content was about 10%. We present herein the proof of principle for the production of dispersed solid state nanoparticles with relevant size and intact plasmid DNA.
Department of Applied Physics, Aalto University School of Science, P.O. Box 15100, 00076 Aalto, Finland. janne.raula@aalto.fi
Janne
J
Raula
Martina
M
Hanzlíková
Antti
A
Rahikkala
Juho
J
Hautala
Esko I
EI
Kauppinen
Arto
A
Urtti
Marjo
M
Yliperttula
D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D005740:Gases; D007930:Leucine / Q000737:chemistry*; D008353:Mannitol / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010957:Plasmids; D011094:Polyethyleneimine / Q000737:chemistry
D005740:Gases; D008353:Mannitol; D011094:Polyethyleneimine; D004247:DNA; D007930:Leucine