Dynamic light scattering application to study protein interactions in electrolyte solutions.
The concentration dependence of the diffusion coefficient of particles suspended in solution depends primarily on the occupied volume fraction and on repulsive and attractive forces. This dependency is expressed by the interaction parameter, which can be assessed experimentally by light scattering measurements and have been determined for the diffusion coefficient of BSA under different salt concentration conditions in the present work. The result shows that the diffusion coefficient of protein grows up with increasing protein concentration, and when the ionic strength turns up gradually the diffusion coefficient decreases with protein concentration's increasing. The concentration dependence of BSA diffusion coefficients is interpreted in the context of a two-body potential of mean force, which includes repulsive hard-sphere and Coulombic interactions and attractive dispersion. With the increase of ionic strength, Debye screening decreases, protein interaction changes from repulsion to attraction, and protein begins to aggregate. By means of the concentration dependence of BSA diffusion coefficients, one can obtain the parameters of protein interactions and can find that protein bears a net effective charge of -9.0 e and has a Hamaker constant of 2.8k(B)T. This work demonstrates that DLS is an effective technique of studying protein interactions.
Institute of Laser Life Science, South China Normal University, Guangzhou, 510631 P.R. China.
Shaoxin
S
Li
Da
D
Xing
Junfeng
J
Li
diffusion coefficient; dynamic light scattering; protein interactions