Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate.
Patterns of the protein streptavidin on DNA origami are printed onto a functionalized surface. The streptavidins are linked to DNA via a disulfide spacer and these conjugates self-organise into a predetermined pattern on DNA origami. The structure immobilises on a biotin-SAM-functionalized Au surface. By disulfide cleavage the origami is separated from the streptavidins, leaving the protein pattern at the surface.
Danish National Research Foundation: Centre for DNA Nanotechnology at Interdisciplinary Nanoscience Center, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark.
Katerina
K
Busuttil
Alexandru
A
Rotaru
Mingdong
M
Dong
Flemming
F
Besenbacher
Kurt V
KV
Gothelf
D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011327:Printing / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
D004220:Disulfides; D001710:Biotin; D004247:DNA; D019809:Streptavidin