Paenisporosarcina indica sp. nov., a psychrophilic bacterium from a glacier, and reclassification of Sporosarcina antarctica Yu et al., 2008 as Paenisporosarcina antarctica comb. nov. and emended description of the genus Paenisporosarcina.
A Gram-stain-positive, aerobic, spore-forming, rod-shaped bacterium, PN2(T), was isolated from a soil sample collected near the Pindari glacier. It contained anteiso-C15 : 0, iso-C15 : 0 and C16 : 1ω7c alcohol as the predominant fatty acids, MK-7 as the major menaquinone and A4α type (l-Lys-d-Glu) peptidoglycan. Based on these characteristics, strain PN2(T) was assigned to the genus Paenisporosarcina. Phylogenetic analysis based on 16S rRNA gene sequence placed strain PN2(T) within the genus Paenisporosarcina and showed a sequence similarity of 98.5-99.0 % with members of this genus. Paenisporosarcina macmurdoensis CMS 21w(T), Paenisporosarcina quisquiliarum SK 55(T) and Sporosarcina antarctica N-05(T) were identified as the most closely related species with 16S rRNA gene sequence similarities of 98.6 %, 99.0 % and 98.4 %, respectively. The values for DNA-DNA relatedness between strain PN2(T) and P. macmurdoensis, P. quisquiliarum and S. antarctica were below the 70 % threshold value (32.0 %, 42.0 % and 38.0 % respectively). In addition, strain PN2(T) exhibited a number of phenotypic differences from P. macmurdoensis, P. quisquiliarum and S. antarctica. Based on the cumulative differences, strain PN2(T) was identified as representing a novel species and the name Paenisporosarcina indica sp. nov. was proposed. The type strain of Paenisporosarcina indica sp. nov. is PN2(T) (LMG 23933(T) = JCM 15114(T)). Furthermore, based on the morphological and chemotaxonomic characteristics, the species Sporosarcina antarctica was reclassified as a species of the genus Paenisporosarcina and renamed Paenisporosarcina antarctica comb. nov. In addition, an emended description of the genus Paenisporosarcina is presented.
Centre for Cellular and Molecular Biology Uppal Road, Hyderabad 500 007, India.
G S N
GSN
Reddy
Centre for Cellular and Molecular Biology Uppal Road, Hyderabad 500 007, India.
B Poorna
BP
Manasa
Centre for Cellular and Molecular Biology Uppal Road, Hyderabad 500 007, India.
Sanjay K
SK
Singh
Centre for Cellular and Molecular Biology Uppal Road, Hyderabad 500 007, India.
S
S
Shivaji
D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D046448:Ice Cover / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D058641:Planococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D058644:Sporosarcina / Q000145:classification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7