In vivo self-bio-imaging of tumors through in situ biosynthesized fluorescent gold nanoclusters.
Fluorescence imaging in vivo allows non-invasive tumor diagnostic thus permitting a direct monitoring of cancer therapies progresses. It is established herein that fluorescent gold nanoclusters are spontaneously biosynthesized by cancerous cell (i.e., HepG2, human hepatocarcinoma cell line; K562, leukemia cell line) incubated with micromolar chloroauric acid solutions, a biocompatible molecular Au(III) species. Gold nanoparticles form by Au(III) reduction inside cells cytoplasms and ultimately concentrate around their nucleoli, thus affording precise cell imaging. Importantly, this does not occur in non-cancerous cells, as evidenced with human embryo liver cells (L02) used as controls. This dichotomy is exploited for a new strategy for in vivo self-bio-imaging of tumors. Subcutaneous injections of millimolar chloroauric acid solution near xenograft tumors of the nude mouse model of hepatocellular carcinoma or chronic myeloid leukemia led to efficient biosynthesis of fluorescent gold nanoclusters without significant dissemination to the surrounding normal tissues, hence allowing specific fluorescent self-bio-marking of the tumors.
State Key Laboratory of Bioelectronics-Chien-Shiung Wu Lab, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Jianling
J
Wang
Gen
G
Zhang
Qiwei
Q
Li
Hui
H
Jiang
Chongyang
C
Liu
Christian
C
Amatore
Xuemei
X
Wang
D003287:Contrast Media / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D006046:Gold / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D020014:K562 Cells; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000473:pathology*; D010316:Particle Size
D003287:Contrast Media; D005456:Fluorescent Dyes; D006046:Gold