Prevention of quorum-sensing-mediated biofilm development and virulence factors production in Vibrio spp. by curcumin.
The increasing occurrence of disease outbreaks caused by Vibrio spp. and the emergence of antibiotic resistance has led to a growing interest in finding alternative strategies to prevent vibriosis. Since the pathogenicity of vibrios is controlled in part by quorum-sensing (QS) system, interfering with this mechanism would prevent the pathogenicity of vibrios without developing resistance. Hence, a non-toxic phytochemical curcumin from Curcuma longa was assessed for its potential in reducing the production of QS-dependent virulence factors in Vibrio spp. The obtained results evidenced 88% reduction in bioluminescence of Vibrio harveyi by curcumin. Further, curcumin exhibited a significant inhibition in alginate, exopolysaccharides, motility, biofilm development and other virulence factors production in Vibrio parahaemolyticus, Vibrio vulnificus and V. harveyi. In in vivo analysis, curcumin enhanced the survival rate of Artemia nauplii up to 67% against V. harveyi infection by attenuating its QS-mediated virulence.
Department of Biotechnology, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Issac Abraham Sybiya Vasantha
IA
Packiavathy
Pitchaikani
P
Sasikumar
Shunmugiah Karutha
SK
Pandian
Arumugam
A
Veera Ravi
D000818:Animals; D001156:Artemia / Q000187:drug effects / Q000382:microbiology / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D053038:Quorum Sensing / Q000187:drug effects*; D014733:Vibrio / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D010936:Plant Extracts; D037521:Virulence Factors; D003474:Curcumin