[Effect of baicalein on proliferation and migration in multiple myeloma cell lines RPMI 8226 and U266 cells].
OBJECTIVE
To investigate the effect of baicalein on proliferation and migration of multiple myeloma (MM) cell lines and its molecular mechanism.


METHODS
The MM cell line RPMI-8226 and U266 cells were used as the model, and treated with different concentration and time of baicalein the effect of baicalein on the MM cells proliferation was assessed by MTT assay. With or without baicalein or Interleukin-6 (IL-6) treatment, the β-catenin protein level was analyzed by immunofluorescence assay and western blot assay and mRNA levels of β-catenin, c-myc, cyclin D1 and integrin 7 gene by RT-PCR. Transwell chamber migration assay was used to detect the cells migration ability with different concentration of baicalein cultured.


RESULTS
Baicalein inhibited the MM cell line RPMI 8226 and U266 cell proliferation in a dose- and time-dependent manner. It simultaneously inhibited β-catenin protein level to resist the effect of IL-6 on inducing MM cell proliferation, and resulted in decrease of β-catenin, c-myc, cyclinD1 and integrin β7 mRNA levels. Baicalein also decreased migration ability of MM cells in a dose-dependent manner by SDF-1.


CONCLUSIONS
Baicalein can inhibit MM cells proliferation and migration, and its molecular mechanisms are associated with inhibition of proliferation related genes β-catenin, c-myc, cyclin D1 and integrin β7 expression.
Department of Hematology, Wuhan University School of Medicine, Wuhan, China.
Chao-ping
CP
Xu
Hui-li
HL
Cai
Li
L
He
Zi
Z
Ma
Shang-qin
SQ
Liu
D015703:Antigens, CD / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism; D015850:Interleukin-6 / Q000494:pharmacology; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051176:beta Catenin / Q000378:metabolism
D015703:Antigens, CD; C530104:CCND1 protein, human; C495270:CTNNB1 protein, human; D044950:Flavanones; D039001:Integrin alpha Chains; D015850:Interleukin-6; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D051176:beta Catenin; C085725:integrin alpha7; D019938:Cyclin D1; C006680:baicalein