C/D box sRNA, CRISPR RNA and tRNA processing in an archaeon with a minimal fragmented genome.
The analysis of deep sequencing data allows for a genome-wide overview of all the small RNA molecules (the 'sRNome') that are present in a single organism. In the present paper, we review the processing of CRISPR (clustered regularly interspaced short palindromic repeats) RNA, C/D box sRNA (small non-coding RNA) and tRNA in Nanoarchaeum equitans. The minimal and fragmented genome of this tiny archaeon permits a sequencing depth that enables the identification of processing intermediates in the study of RNA processing pathways. These intermediates include circular C/D box sRNA molecules and tRNA half precursors.
Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 10, 35037 Marburg, Germany.
Hagen
H
Richter
Sabine
S
Mohr
Lennart
L
Randau
D001105:Archaea / Q000235:genetics*; D020745:Genome, Archaeal; D012323:RNA Processing, Post-Transcriptional; D019642:RNA, Archaeal / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*
D019642:RNA, Archaeal; D012343:RNA, Transfer