The regenerated bone quality by implantation of octacalcium phosphate collagen composites in a canine alveolar cleft model.
OBJECTIVE
Synthetic octacalcium phosphate and porcine atelocollagen composites significantly enhanced bone regeneration more than β-tricalcium phosphate collagen composite and hydroxyapatite collagen composite in a rat cranial defect model. However, the long-term stability and quality of octacalcium phosphate collagen (OCP/Col) composites-derived regenerated bone, when implanted in a canine alveolar cleft model, have yet to be elucidated. The present study investigated the longterm stability and quality of bone regenerated by OCP/Col.


METHODS
Disks of OCP/Col or collagen were implanted in a canine alveolar-cleft model (n = 6). Then, bone regeneration in the implanted areas was investigated macroscopically, radiographically, and histologically at 10 months after implantation. In addition, three-dimensional quantitative images of regenerated bone were analyzed by microcomputed tomography.


RESULTS
Macroscopically, the OCP/Col treated alveolus was clearly augmented, and radio-opacity in the OCP/Col implanted area was comparable to that of the original alveolus bone. On histological analysis, the area was mostly filled with newly formed bone, and a few granules of implanted OCP/Col were enclosed in it. In the microcomputed tomography analysis, the regenerated bone volume in the OCP/Col group was larger than that in the collagen group. OCP/Col-derived bone consisted of outer cortical and inner cancellous structure with dense trabeculae and seemed like the original bone structure.


CONCLUSIONS
OCP/Co composites could be a useful bone regenerative material to substitute for autogenous bone because their implantation could elicit high bone regeneration and active structural reconstitution.
Aritsune
A
Matsui
Keiko
K
Matsui
Takuto
T
Handa
Yuji
Y
Tanuma
Kei-Ichiro
K
Miura
Yuta
Y
Kato
Tadashi
T
Kawai
Osamu
O
Suzuki
Shinji
S
Kamakura
Seishi
S
Echigo
D000539:Alveolar Process / Q000002:abnormalities* / Q000601:surgery*; D000543:Alveoloplasty / Q000379:methods*; D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D004338:Drug Combinations
D018786:Bone Substitutes; D002130:Calcium Phosphates; D004338:Drug Combinations; C022045:octacalcium phosphate; D003094:Collagen
alveolar cleft; bone regeneration; collagen; dog; octacalcium phosphate