Measuring protein reduction potentials using 15N HSQC NMR spectroscopy.
NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple (15)N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxidoreductase of comparable or greater reducing strength than PDI a.
School of Bioscience, University of Kent, Canterbury, Kent, CT2 7NJ, UK.
Samantha L
SL
Taylor
Harriet
H
Crawley-Snowdon
Jane L
JL
Wagstaff
Michelle L
ML
Rowe
Mark
M
Shepherd
Richard A
RA
Williamson
Mark J
MJ
Howard
D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D005978:Glutathione / Q000737:chemistry; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D019704:Protein Disulfide-Isomerases / Q000737:chemistry* / Q000378:metabolism
D029968:Escherichia coli Proteins; D009587:Nitrogen Isotopes; D019704:Protein Disulfide-Isomerases; C526648:dsbA protein, E coli; D005978:Glutathione