Alphavbeta3 suppresses the RhoA-LIMK1 pathway in K1735 melanoma.
Mucocutaneous melanoma has a five-year survival rate of less than 10 percent. The alphavbeta3 integrin promotes invasion, which requires actin reorganization by cofilin. The authors previously showed that cofilin and alphavbeta3 promote invasion. K1735 melanoma has several clones, each with different levels of alphavbeta3. The authors found that expression of alphavbeta3 suppresses activation of RhoA thus inhibiting LIMK1 phosphorylation of cofilin. This indicates that alphavbeta3 integrin suppresses the RhoA/ ROCK/LIMK1 pathway.
University of California, Davis, USA.
Casey
C
Lee
Amanda
A
Siu
Joy
J
Chang
Dongmin
D
Dang
Daniel M
DM
Ramos
D051339:Actin Depolymerizing Factors / Q000378:metabolism / Q000502:physiology; D000577:Amides / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation; D039302:Integrin alphaVbeta3 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D054461:Lim Kinases / Q000378:metabolism / Q000502:physiology*; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D008856:Microscopy, Fluorescence; D009361:Neoplasm Invasiveness; D010749:Phosphoprotein Phosphatases / Q000378:metabolism / Q000502:physiology; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D020741:rho GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D020742:rhoA GTP-Binding Protein
D051339:Actin Depolymerizing Factors; D000577:Amides; D004791:Enzyme Inhibitors; D039302:Integrin alphaVbeta3; D011725:Pyridines; C108830:Y 27632; D054461:Lim Kinases; C515893:Limk1 protein, mouse; D010749:Phosphoprotein Phosphatases; C529575:SSH1 protein, mouse; C526380:RhoA protein, mouse; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein