Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation.
In the present study, we developed an efficient method of 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of Klebsiella pneumoniae AK mutant strains. The proposed approach eliminated by-product formation and IPTG induction resulted in maximal production of 1,3-PD. A series of recombinant strains was designed to constitutively express the dhaB and/or dhaT genes, using the bacteriophage T5 P(DE20) promoter and the rho-independent transcription termination signal of the Rahnella aquatilis levansucrase gene. Among these strains, AK/pConT expressing dhaT alone gave the highest yield of 1,3-PD. Fed-batch fermentation resulted in efficient production of 1,3-PD from either pure or crude glycerol, without by-product formation.
Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea.
Baek-Rock
BR
Oh
Jeong-Woo
JW
Seo
Sun-Yeon
SY
Heo
Lian Hua
LH
Luo
Won-Kyung
WK
Hong
Don-Hee
DH
Park
Chul-Ho
CH
Kim
D000426:Alcohol Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism; D003451:Cryoprotective Agents / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D005990:Glycerol / Q000378:metabolism* / Q000494:pharmacology; D006602:Hexosyltransferases / Q000096:biosynthesis / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D011401:Promoter Regions, Genetic; D011409:Propylene Glycols / Q000378:metabolism*; D020638:Rahnella / Q000201:enzymology / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D003451:Cryoprotective Agents; D011409:Propylene Glycols; D014764:Viral Proteins; C041787:1,3-propanediol; D000426:Alcohol Dehydrogenase; C052370:1,3-propanediol dehydrogenase; D006602:Hexosyltransferases; C021513:levansucrase; D005990:Glycerol