β-Arrestin-1 directly interacts with Gαs and regulates its function.
β-Arrestins function to mediate G protein-coupled receptor (GPCR) desensitization and internalization and to initiate G protein independent signaling of GPCRs. Elucidating how β-arrestin and G protein signal pathways coordinate with each other is important to fully understand GPCR signaling. Here we report that β-arrestin-1 directly interacts with Gα(s). Purified β-arrestin-1 binds to Gα(s) in a rapid association and dissociation manner. β-Arrestin-1 promotes the binding and the release of GTPγS from Gα(s) in vitro. β-Arrestin-1 L33K mutant shows reduced interaction with Gα(s) and has no detectable effects on Gα(s) function. Our study thus reveals a direct crosstalk of β-arrestin-1 with Gα(s).
State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, PR China.
Bo
B
Li
Congcong
C
Wang
Zhaocai
Z
Zhou
Jian
J
Zhao
Gang
G
Pei
D019943:Amino Acid Substitution; D000818:Animals; D019390:Arrestins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D044385:GTP-Binding Protein alpha Subunits / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000737:chemistry; D006153:Guanosine Diphosphate / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D007368:Interferometry; D007700:Kinetics; D008567:Membranes, Artificial; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D025941:Protein Interaction Mapping; D051381:Rats; D000071559:beta-Arrestin 1; D000071557:beta-Arrestins
C000605253:ARRB1 protein, human; C000605256:Arrb1 protein, rat; D019390:Arrestins; D044385:GTP-Binding Protein alpha Subunits; D008567:Membranes, Artificial; D000071559:beta-Arrestin 1; D000071557:beta-Arrestins; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate)