Sequencing ASMT identifies rare mutations in Chinese Han patients with autism.
Melatonin is involved in the regulation of circadian and seasonal rhythms and immune function. Prior research reported low melatonin levels in autism spectrum disorders (ASD). ASMT located in pseudo-autosomal region 1 encodes the last enzyme of the melatonin biosynthesis pathway. A previous study reported an association between ASD and single nucleotide polymorphisms (SNPs) rs4446909 and rs5989681 located in the promoter of ASMT. Furthermore, rare deleterious mutations were identified in a subset of patients. To investigate the association between ASMT and autism, we sequenced all ASMT exons and its neighboring region in 398 Chinese Han individuals with autism and 437 healthy controls. Although our study did not detect significant differences of genotypic distribution and allele frequencies of the common SNPs in ASMT between patients with autism and healthy controls, we identified new rare coding mutations of ASMT. Among these rare variants, 4 were exclusively detected in patients with autism including a stop mutation (p.R115W, p.V166I, p.V179G, and p.W257X). These four coding variants were observed in 6 of 398 (1.51%) patients with autism and none in 437 controls (Chi-Square test, Continuity Correction p = 0.032, two-sided). Functional prediction of impact of amino acid showed that p.R115W might affect protein function. These results indicate that ASMT might be a susceptibility gene for autism. Further studies in larger samples are needed to better understand the degree of variation in this gene as well as to understand the biochemical and clinical impacts of ASMT/melatonin deficiency.
Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing, People's Republic of China. lifangwang@bjmu.edu.cn
Lifang
L
Wang
Jun
J
Li
Yanyan
Y
Ruan
Tianlan
T
Lu
Chenxing
C
Liu
Meixiang
M
Jia
Weihua
W
Yue
Jing
J
Liu
Thomas
T
Bourgeron
Dai
D
Zhang
D000121:Acetylserotonin O-Methyltransferase / Q000235:genetics*; D000293:Adolescent; D044466:Asian People / Q000208:ethnology* / Q000235:genetics*; D001321:Autistic Disorder / Q000201:enzymology / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D009154:Mutation
C523771:ASMT protein, human; D000121:Acetylserotonin O-Methyltransferase