Interaction between lung cancer cell and myofibroblast influenced by cyclic tensile strain.
Using a cell culture chip with a deformable substrate driven by a hydraulic force, we investigated the motility of cancer cells affected by myofibroblasts undergoing cyclic tensile strain (CTS). CTS reduced both the expression of α-smooth muscle actin in the myofibroblast and the ability of the myofibroblast to accelerate cancer cell migration. However, with the treatment of a pro-inflammatory factor interleukin-1β on the myofibroblasts, the effects of CTS on the myofibroblast were diminished. This result suggests an antagonism between mechanical and chemical stimulations on mediating cancer metastasis by the stromal myofibroblast.
Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 20224, Taiwan.
Jiun-Wei
JW
Huang
Huei-Jyuan
HJ
Pan
Wei-Yu
WY
Yao
Yu-Wei
YW
Tsao
Wei-Yu
WY
Liao
Chih-Wei
CW
Wu
Yi-Chung
YC
Tung
Chau-Hwang
CH
Lee
D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D058628:Myofibroblasts / Q000166:cytology / Q000378:metabolism*; D041681:NIH 3T3 Cells; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D000199:Actins; D053583:Interleukin-1beta; D053773:Transforming Growth Factor beta1