Protein immobilization on 3C-SiC (100) as a substrate for detecting the onset of acute myocardial infarction (AMI).
Silicon Carbide (SiC), has been shown to be a bio- and hema-compatible substrate that could potentially be used in biosensor applications. The development of a viable biorecognition interface using SiC as the substrate material for bio-detection is described. Surface modification with 3-aminopropyltriethoxysilane (APTES) and immobilization via covalent conjugation of antimyoglobin (anti-Myo) on the modified surfaces is achieved, which are initial steps for immunosensing based devices. Successful formation of APTES layers and antibody immobilization were identified with surface water contact angle (SWCA), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM).
Electrical Engineering Department, University of South Florida, Tampa, FL 33620 USA. amolive4@mail.usf.edu
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Oliveros
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Guiseppi-Elie
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Jaroszeski
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Saddow
D002244:Carbon / Q000378:metabolism*; D017554:Carbon Compounds, Inorganic / Q000378:metabolism*; D006900:Hydroxylation; D055767:Immobilized Proteins / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D009203:Myocardial Infarction / Q000175:diagnosis*; D009211:Myoglobin / Q000378:metabolism*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D017655:Silicon Compounds / Q000378:metabolism*; D013499:Surface Properties
D017554:Carbon Compounds, Inorganic; D055767:Immobilized Proteins; D009211:Myoglobin; D011437:Propylamines; D012821:Silanes; D017655:Silicon Compounds; D002244:Carbon; C477625:amino-propyl-triethoxysilane; C022088:silicon carbide