Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera.
Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutralization responses generated during natural viral infection. Additionally, Ad5-specific NAbs against the fiber knob protein were present in over 90% of the positive serum samples while 42% of the sera had NAbs against hexon in this study based on neutralization assay of anti-HVR and anti-knob subtracted sera and Western blotting analysis. We also found that the trimeric knob was preferentially recognized by fiber-induced NAbs and it was serotype-specific in human adenovirus species C. Results indicated that the trimeric knob protein would be a good candidate antigen for detecting adenovirus serotype-specific NAbs in naturally infected sera.
National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, China.
Bin
B
Yu
Jianing
J
Dong
Chu
C
Wang
Yang
Y
Zhan
Haihong
H
Zhang
Jiaxin
J
Wu
Wei
W
Kong
Xianghui
X
Yu
D000256:Adenoviridae / Q000276:immunology*; D000257:Adenoviridae Infections / Q000453:epidemiology* / Q000662:veterinary*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D015153:Blotting, Western; D036022:Capsid Proteins / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests; D016036:Seroepidemiologic Studies
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins