Toxicity assessment of transgenic papaya ringspot virus of 823-2210 line papaya fruits.
The transgenic papaya is a valuable strategy for creating plants resistant to papaya ringspot virus (PRSV) infection and increasing production. This study was further performed to evaluate the comparative toxicity effects of the newly developed transgenic line of the fruits of two backcross transgenic papaya lines (2210 and 823) and one hybrid line (823-2210) and compare to their parent non-transgenic (TN-2) counterparts. The stability analysis of coat protein (CP) of PRSV was investigated using the digestion stability assays in simulated gastric fluid (SGF), simulated intestinal fluid (SIF), and bile salts to detect the CP fragments. Results revealed that the CP fragments were rapidly hydrolyzed in SGF and were undetectable in organs and gastrointestinal contents in rats. For the genotoxicity, three in vitro assays were conducted and exhibited that non-transgenic and backcross transgenic papaya fruits were negative. Moreover, a repeated animal feeding study was conducted by feeding 2 g/kg of body weight (bw) of non-transgenic and backcross transgenic papaya fruits for 28 days in rats. There were no biological or toxicological significances between non-transgenic and backcross transgenic papaya fruits in rats. The results demonstrated that the backcross transgenic papaya fruit can be recognized as an equivalent substitution for traditional papaya in food safety.
College of Bioresources, National I-Lan University, Taiwan, Republic of China.
Hsin-Tang
HT
Lin
Gow-Chin
GC
Yen
Ting-Tzu
TT
Huang
Lit-Fu
LF
Chan
Ying-Huey
YH
Cheng
Jhaol-Huei
JH
Wu
Shyi-Dong
SD
Yeh
Sheng-Yang
SY
Wang
Jiunn-Wang
JW
Liao
D000821:Animal Feed; D000818:Animals; D029441:Carica / Q000235:genetics / Q000633:toxicity* / Q000821:virology; D005260:Female; D005638:Fruit / Q000235:genetics / Q000633:toxicity* / Q000821:virology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D030821:Plants, Genetically Modified / Q000633:toxicity* / Q000821:virology; D017800:Potyvirus; D051381:Rats; D017207:Rats, Sprague-Dawley