Lipase-immobilized biocatalytic membranes for biodiesel production.
Microbial lipase from Candida rugosa (Amano AY-30) has good transesterification activity and can be used for biodiesel production. In this study, polyvinylidene fluoride (PVDF) membrane was grafted with 1,4-diaminobutane and activated by glutaraldehyde for C. rugosa lipase immobilization. After immobilization, the biocatalytic membrane was used for producing biodiesel from soybean oil and methanol via transesterification. Response Surface Methodology (RSM) in combination with a 5-level-5-factor central composite rotatable design (CCRD) was employed to evaluate the effects of reaction time, reaction temperature, enzyme amount, substrate molar ratio and water content on the yield of soybean oil methyl ester. By ridge max analysis, the predicted and experimental yields under the optimum synthesis conditions were 97% and 95%, respectively. The lipase-immobilized PVDF membrane showed good reuse ability for biodiesel production, enabling operation for at least 165 h during five reuses of the batch, without significant loss of activity.
Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
Chia-Hung
CH
Kuo
Li-Ting
LT
Peng
Shu-Chen
SC
Kan
Yung-Chuan
YC
Liu
Chwen-Jen
CJ
Shieh
D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D004951:Esterification; D008049:Lipase / Q000378:metabolism*; D008567:Membranes, Artificial; D011145:Polyvinyls; D011700:Putrescine; D013024:Soybean Oil / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D005465:Fluorocarbon Polymers
D056804:Biofuels; D008049:Lipase; D008567:Membranes, Artificial; D011145:Polyvinyls; D011700:Putrescine; D013024:Soybean Oil; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C081378:solysime
Biodiesel; Immobilized lipase; Polyvinylidene fluoride membrane; Response surface methodology