Chemo protective activity of carotenoid meso-zeaxanthin against doxorubicin-induced cardio toxicity.
Carotenoid meso-zeaxanthin (MZ) was evaluated for its protective effect against doxorubicin (DOX) induced cardio toxicity in Wistar rats. Oral administration of MZ was started 15 days prior to doxorubicin (30mg/kg b.wt, i.p, single dose) injection. Animals in all groups were sacrificed 24 hours after doxorubicin administration. Serum markers of cardiac injury-LDH, CPK, SGOT and SGPT levels, which were increased drastically by doxorubicin treatment, were decreased to normal level by MZ pre-treatment. MZ significantly mitigated DOX-provoked reductions of cardiac activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), as well as depletion of glutathione (GSH).Pretreatment of MZ significantly guarded against DOX-induced rise of oxidative stress markers like lipid peroxidation (LPO), tissue hydroperoxides (HP) and conjugated dienes (CD) in cardiac tissue. Moreover, DOX-induced myocardial injury, which was manifested by arrhythmias and conduction abnormalities in ECG (increased ST, QT interval and ST elevation), was reversed significantly by MZ pre-treatment. Histopathological analysis further confirmed cardio-protective potential of this carotenoid.
Amala Cancer Research Centre, India.
Alikunjhi P
AP
Firdous
Ramadasan
R
Kuttan
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001145:Arrhythmias, Cardiac / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D009202:Cardiomyopathies / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D002374:Catalase / Q000378:metabolism; D004317:Doxorubicin / Q000633:toxicity*; D004562:Electrocardiography; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D024341:Xanthophylls / Q000627:therapeutic use*; D065146:Zeaxanthins
D000903:Antibiotics, Antineoplastic; D024341:Xanthophylls; D065146:Zeaxanthins; C584722:meso-zeaxanthin; D008315:Malondialdehyde; D004317:Doxorubicin; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione