Microplate alamarBlue assay for Paracoccidioides susceptibility testing.
CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs.
Faculdade de Ciências Farmacêuticas, UNESP-Universidade Estadual Paulista, Araraquara, Departamento de Analises Clinicas, Laboratório de Micologia Clínica, São Paulo, Brazil.
A C A
AC
de Paula e Silva
H C
HC
Oliveira
J F
JF
Silva
F
F
Sangalli-Leite
L
L
Scorzoni
A M
AM
Fusco-Almeida
M J S
MJ
Mendes-Giannini
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D010078:Oxazines / Q000378:metabolism*; D010228:Paracoccidioides / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D014966:Xanthenes / Q000378:metabolism*
D000935:Antifungal Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D017964:Itraconazole; D000666:Amphotericin B