Isolation and purification of a peptide from Bullacta exarata and its impaction of apoptosis on prostate cancer cell.
Bullacta exarata was hydrolyzed with trypsin to prepare peptides; Hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. The purity of the Bullacta exarata was demonstrated by HPLC and its peptide sequence analysis was detected. The effects of BEPT II and BEPT II-1 on the proliferation of PC-3 cells were examined using a MTT assay. BEPT II and BEPT II-1 significantly inhibited the proliferation of PC-3 cells in a time- and dose-dependent manner. Annexin V/PI double staining studies showed exposing PC-3 cells to 5, or 15 mg/mL BEPT II-1 for 24 h increased the percentage of the early stage of apoptotic cells from 11.22% to 22.09%.  In addition, typical morphologic changes were observed in the cells with  acridine orange/ethidium bromide staining. These data support that BEPT II-1 has  anticancer properties and merits further investigation to understand the mechanisms of  BEPT II-1-induced apoptosis in PC-3 cells.
School of Food Science and Pharmacy, Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Zhejiang Ocean University, Zhoushan 316000, China. majyin2012@gmail.com
Jianyin
J
Ma
Fangfang
F
Huang
Huanle
H
Lin
Xian
X
Wang
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D049851:Gastropoda / Q000737:chemistry*; D006801:Humans; D008297:Male; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000970:Antineoplastic Agents; D010455:Peptides