Molecular cloning and characterization of the  pseudorabies virus US1 gene.
Using polymerase chain reaction, a 1050-bp sequence of the US1 gene was amplified from the pseudorabies virus (PRV) Becker strain genome; identification of the US1 gene was confirmed by further cloning and sequencing. Bioinformatics analysis indicated that the PRV US1 gene encodes a putative polypeptide with 349 amino acids. The encoded protein, designated PICP22, had a conserved Herpes_IE68 domain, which was found to be closely related with the herpes virus immediate early regulatory protein family and is highly conserved among the counterparts encoded by Herpes_IE68 genes. Multiple nucleic acid sequence and amino acid sequence alignments suggested that the product of PRV US1 has a relatively higher homology with ICP22-like proteins of genus Varicellovirus than with those of other genera of Alphaherpesvirinae. In addition, phylogenetic analysis showed that PRV US1 has a close evolutionary relationship with members of the genus Varicellovirus, especially Equid herpes virus 1 (EHV-1), EHV-4 and EHV-9. Antigen prediction indicated that several potential B-cell epitopes are located in PICP22. Also, subcellular localization analysis demonstrated that PICP22 is predominantly located in the cytoplasm, suggesting that it might function as a cytoplasmic-targeted protein.
Department of Pathogenic Biology and Immunology, Guangzhou Medical University, China.
M L
ML
Li
J H
JH
Chen
Z Y
ZY
Zhao
K J
KJ
Zhang
Z
Z
Li
J
J
Li
J Y
JY
Mai
X M
XM
Zhu
M S
MS
Cai
D018138:Alphaherpesvirinae / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D004279:DNA, Viral / Q000235:genetics*; D018985:Epitopes, B-Lymphocyte / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D018140:Varicellovirus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D018985:Epitopes, B-Lymphocyte; C057294:ICP22 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D014764:Viral Proteins