Kinetics and thermodynamics of DNA, RNA, and hybrid duplex formation.
The rates of duplex formation for two octamers of DNA (5' d-CACGGCTC/5' d-GAGCCGTG and 5' d-CACAGCAC/5' d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, ΔH° and ΔS°, have been determined for the same sequences under the same buffer conditions using optical melting techniques. These data reveal a linear free energy relationship between the free energy of activation for denaturation and the change in free energy for formation of the duplexes. This relationship indicates that these duplex formation reactions occur through a common unstructured transition state that is more similar to the single strands in solution than to the ensuing duplex. In addition, these data confirm that the greater stability of RNA duplexes relative to that of homologous DNA and hybrid duplexes is controlled by the denaturation rate and not the duplex formation rate.
Department of Chemistry, Allegheny College , 520 North Main Street, Meadville, Pennsylvania 16335, United States.
Brittany
B
Rauzan
Elizabeth
E
McMichael
Rachel
R
Cave
Lesley R
LR
Sevcik
Kara
K
Ostrosky
Elisabeth
E
Whitman
Rachel
R
Stegemann
Audra L
AL
Sinclair
Martin J
MJ
Serra
Alice A
AA
Deckert
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000737:chemistry*; D020871:RNA Stability; D013816:Thermodynamics
D012313:RNA; D004247:DNA