Direct assessment of metabolite utilization by Pseudomonas aeruginosa during growth on artificial sputum medium.
We grew Pseudomonas aeruginosa in LB and artificial sputum medium (ASM) (filtered and unfiltered) and quantified metabolite utilization and excretion by nuclear magnetic resonance (NMR) spectroscopy (metabolic footprinting or extracellular metabolomics). Utilization rates were similar between media, but there were differences in excretion-e.g., acetate was produced only in unfiltered ASM.
Department of Surgery and Cancer, Imperial College London, London, United Kingdom.
Volker
V
Behrends
Benedikt
B
Geier
Huw D
HD
Williams
Jacob G
JG
Bundy
D003470:Culture Media / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D008962:Models, Theoretical; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000378:metabolism*; D013183:Sputum / Q000382:microbiology*
D003470:Culture Media