Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells.
Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promoters for a Bombyx mori Tet-on system. In order to further improve this system, Giant, a transcriptional silencer from Drosophila melanogaster, is introduced to repress leaky transcription in the absence of doxycycline. Further, the promoter responsibility to the tetracycline-controlled transactivators is facilitated by introducing a synthetic minimal core promoter. With the tightly regulated second-generation silkworm Tet-on system, we obtain up to 300-fold induction of gene expression with the addition of doxycycline.
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Fukuoka, Japan.
Tsuneyuki
T
Tatsuke
Jae Man
JM
Lee
Takahiro
T
Kusakabe
Kazuhiro
K
Iiyama
Hideki
H
Sezutsu
Keiro
K
Uchino
D000818:Animals; D012831:Bombyx / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005821:Genetic Techniques; D019913:Nuclear Localization Signals; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D013752:Tetracycline; D015534:Trans-Activators; D014158:Transcription, Genetic
D019913:Nuclear Localization Signals; D015534:Trans-Activators; D013752:Tetracycline