Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T.
Fe(3)O(4)@SiO(2) core-shell nanoparticles were synthesized and used to label human mesenchymal stem cells (hMSCs) for in vitro and in vivo magnetic resonance imaging study. The diameter of the nanoparticles is 24-30 nm with a Fe(3)O(4) core of ∼8 nm and a SiO(2) shell of ~8 nm. Transverse relaxivity of the nanoparticles dispersed in water is measured to be ~106 mM(-1) s(-1). After incubation with hMSCs for 12 h at a concentration of 100 μg Fe/mL, cellular uptake of Fe(3)O(4)@SiO(2) is 20-100 pg Fe/cell, which are located predominantly in the cytoplasm of cells. This level of uptake exhibits no significant influence on hMSCs' viability and differentiation. In vitro imaging of Fe(3)O(4)@SiO(2)-labeled hMSCs evenly distributed in agarose gel yields single cell sensitivity at 11.7 T. In vivo imaging of Fe(3)O(4)@SiO(2)-labeled hMSCs injected into the left brain hemisphere of nude mice yields imaging sensitivity of ~130 hMSCs.
Division of Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, PR China.
Peiwei
P
Yi
Guangcun
G
Chen
Hailu
H
Zhang
Fei
F
Tian
Bo
B
Tan
Jianwu
J
Dai
Qiangbin
Q
Wang
Zongwu
Z
Deng
D000818:Animals; D002478:Cells, Cultured; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D012822:Silicon Dioxide / Q000737:chemistry*
D005290:Ferric Compounds; C000499:ferric oxide; D012822:Silicon Dioxide