Inhibitory potential of fatty acids on key enzymes related to type 2 diabetes.
This study aimed to examine the inhibitory mechanisms of fatty acids on key enzymes related to type 2 diabetes, and their effects on starch digestion rate. Among the 10 fatty acids analyzed, oleic acid showed the strongest anti-α-glucosidase activity, followed by linoleic acid, and their activities were more potent than acarbose, but they possessed a weaker anti-α-amylase activity. Kinetic assays demonstrated that oleic acid and linoleic acid were competitive inhibitors, and their interactions with α-glucosidase exhibited a character of static quenching, which indicates that they would bind to α-glucosidase to form a complex. However, they had little effects on the secondary structures of α-glucosidase. In vitro study showed that oleic acid and linoleic acid were more potent than acarbose in inhibiting starch digestion. Taken together, these results conclude that oleic acid and linoleic acid possess potent inhibitory effects on α-glucosidase activity.
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.
Chun-Han
CH
Su
Chun-Hua
CH
Hsu
Lean-Teik
LT
Ng
D002942:Circular Dichroism; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000201:enzymology; D005227:Fatty Acids / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D006868:Hydrolysis; D007004:Hypoglycemic Agents / Q000737:chemistry*; D007700:Kinetics; D017433:Protein Structure, Secondary; D013213:Starch / Q000737:chemistry; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000737:chemistry*
D005227:Fatty Acids; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D013213:Starch; D000516:alpha-Amylases; D000520:alpha-Glucosidases
fatty acids; linoleic acid; oleic acid; type 2 diabetes; α-amylase; α-glucosidase