[Schistosomicidal effect of BTW5 on adult and juvenile Schistosoma japonicum in vitro].
OBJECTIVE
To evaluate the killing effect of BTW5 on both juvenile and adult worms of Schistosoma japonicum in vitro.


METHODS
The mice were infected with cercariae of Schistosoma japonicum obtained from infected Oncomelania hupensis. The juvenile worms were obtained from the liver and mesenteric veins of the mice by perfusion 18 d after the infection, and the adult worms were obtained from the liver and mesenteric veins of the mice by perfusion 5 weeks after the infection. The adult and juvenile worms were cultivated in DMEM medium containing different concentrations of BTW5 and were observed for 3 d. After the observation, the worms were stained by hydrochloric acid carmine to observe the injuries.


RESULTS
The death rates and motility reducing rates of adult and juvenile worms in DMEM medium containing different concentrations of BTW5 were significantly higher than those in the controls. The staining suggested that BTW5 had a damage effect on the tegument and the intestinal canal of the male and female worms, in addition to the ovaries of the female worms.


CONCLUSIONS
BTW5 has an antischistosomal effect on adult and juvenile worms of Oncomelania hupensis.
Department of Pathogenic Biology, School of Basic Medicine and Bioscience, Medical College of Soochow University, Suzhou 215123, China.
Chen-Chen
CC
Liu
Jing
J
Zhang
Si-Jia
SJ
Gao
Xia
X
Zhou
Wei
W
Gong
Shi-Lin
SL
Yang
Qiong-Ming
QM
Xu
Hong-Xiang
HX
Zhu-Ge
D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D012549:Schistosoma japonicum / Q000187:drug effects* / Q000254:growth & development; D012554:Schistosomiasis japonica / Q000469:parasitology*; D012556:Schistosomicides / Q000494:pharmacology*
D012556:Schistosomicides