[Cytocompatibility of chitosan-based thermosensitive hydrogel to human periodontal ligament cell].
OBJECTIVE
The aim of this investigation was to evaluate the cytocompatibility of an in situ chitosan-quaternized chitosan/α, β-glycerophosphate (CS-HTCC/GP) thermosensitive hydrogel in vitro.


METHODS
The primary cells were isolated from human periodontal ligament and cultured. The role of different concentrations of CS-HTCC/GP extract to HPDLCs was evaluated by MTT assay and alkaline phosphatase (ALP) activity. Also, the ultra-architecture of HPDLCs was determined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) respectively. SPSS13.0 software package was used for statistical analysis.


RESULTS
By immunocytochemical method, the cells were stained positively to antibodies against vimentin, and negatively to antibodies against cytokeratin, which indicated that they were external embryo mesenchymal cell without epithelial cell mixure. CS-HTCC/GP thermosensitive hydrogel promoted proliferation of HPDLCs,especially at 3d and 5d, the results was significantly different (P<0.001). ALP activity was significantly greater in group 2 and 3 than in group 4 after 5d (P<0.001). Also, no negative influence to ultrastructure of HPDLCs was found through SEM and TEM.


CONCLUSIONS
The results indicate that CS-HTCC/GP thermosensitive hydrogel exhibits excellent cytocompatibility and has potential to be used as an in situ injectable local periodontal drug delivery vehicle and a tissue-engineering scaffold for periodontal disease therapy.
Department of Stomatology, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China. jqx_1@163.com
Qiu-xia
QX
Ji
Xin-bo
XB
Yu
Quan-chen
QC
Xu
Hong
H
Wu
D048271:Chitosan; D005994:Glycerophosphates; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D059630:Mesenchymal Stem Cells; D010510:Periodontal Diseases; D010513:Periodontal Ligament
D005994:Glycerophosphates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; C031463:beta-glycerophosphoric acid