Biochemical evaluation of triploid progenies of diploid × tetraploid breeding populations of Camellia for genotypes rich in catechin and caffeine.
To verify the quality of triploid varieties of Camellia tea species at the secondary metabolite level, we tested caffeine and catechin profiles of 97 F(1) segregating progenies in two breeding populations with a common tetraploid parent and diploid parents of two geographic and varietal origins. Catechin and caffeine levels of the triploid progenies were quantified and compared against their diploid parent. Some of the progenies showed better performance than their diploid parent. Most of the progenies of the diploid C. sinensis × tetraploid cross showed heterosis for caffeine and EGCG. Progenies of the C. assamica subsp. lasiocalyx × tetraploid cross showed heterosis for +C, EC, EGC, and TC. The genomic contributions of the diploid parent seem to be the main factor in the variation between the two populations. Our studies showed quantitative enhancement of some of the quality-related parameters in tea, providing a platform to refocus on this classical breeding approach for developing quality cultivars in tea.
Department of Biotechnology, Jagannath Barooah College, Jorhat 785001, Assam, India. sourabhkdas@yahoo.com
Sourabh Kumar
SK
Das
Santanu
S
Sabhapondit
Giasuddin
G
Ahmed
Sudripta
S
Das
D001947:Breeding; D002110:Caffeine / Q000378:metabolism*; D028244:Camellia / Q000235:genetics*; D002392:Catechin / Q000378:metabolism*; D032461:Chromosomes, Plant / Q000235:genetics; D016001:Confidence Intervals; D003433:Crosses, Genetic; D004171:Diploidy; D005838:Genotype; D006823:Hybrid Vigor; D008677:Metaphase / Q000235:genetics; D025341:Principal Component Analysis; D057891:Tetraploidy; D057885:Triploidy
D002110:Caffeine; D002392:Catechin