Modeling of lamin A/C mutation premature cardiac aging using patient‐specific induced pluripotent stem cells.
OBJECTIVE
We identified an autosomal dominant non‐sense mutation (R225X) in exon 4 of the lamin A/C (LMNA) gene in a Chinese family spanning 3 generations with familial dilated cardiomyopathy (DCM). In present study, we aim to generate induced pluripotent stem cells derived cardiomyocytes (iPSC‐CMs) from an affected patient with R225X and another patient bearing LMNA frame‐shift mutation for drug screening.


RESULTS
Higher prevalence of nuclear bleb formation and micronucleation was present in LMNA(R225X/WT) and LMNA(Framshift/WT) iPSC‐CMs. Under field electrical stimulation, percentage of LMNA‐mutated iPSC‐CMs exhibiting nuclear senescence and cellular apoptosis markedly increased. shRNA knockdown of LMNA replicated those phenotypes of the mutated LMNA field electrical stress. Pharmacological blockade of ERK1/2 pathway with MEK1/2 inhibitors, U0126 and selumetinib (AZD6244) significantly attenuated the pro‐apoptotic effects of field electric stimulation on the mutated LMNA iPSC‐CMs.


CONCLUSIONS
LMNA‐related DCM was modeled in‐vitro using patient‐specific iPSC‐CMs. Our results demonstrated that haploinsufficiency due to R225X LMNA non‐sense mutation was associated with accelerated nuclear senescence and apoptosis of iPSC‐ CMs under electrical stimulation, which can be significantly attenuated by therapeutic blockade of stress‐related ERK1/2 pathway.
Cardiology Division, Department of Medicine, Queen Mary Hospital, the University of Hong Kong, Hong Kong SAR, China.
Chung-Wah
CW
Siu
Yee-Ki
YK
Lee
Jenny Chung-Yee
JC
Ho
Wing-Hon
WH
Lai
Yau-Chi
YC
Chan
Kwong-Man
KM
Ng
Lai-Yung
LY
Wong
Ka-Wing
KW
Au
Yee-Man
YM
Lau
Jinqiu
J
Zhang
Kenneth Weijian
KW
Lay
Alan
A
Colman
Hung-Fat
HF
Tse
D000375:Aging / Q000502:physiology*; D015153:Blotting, Western; D002311:Cardiomyopathy, Dilated / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D034904:Lamin Type A / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009154:Mutation; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010375:Pedigree
C494847:LMNA protein, human; D034904:Lamin Type A