FRET based ratio-metric sensing of hyaluronidase in synthetic urine as a biomarker for bladder and prostate cancer.
Elevated hyaluronidase levels are found in the urine of bladder and prostate cancer patients. Therefore, HA-ase is regarded as an important biomarker for the detection of these cancers. In this report, we use a FRET based ratiometric sensing approach to detect the level of HA-ase in synthetic urine. For this, we have used a HA-FRET probe (hyaluronan) labeled with fluorescein as a donor and rhodamine as an acceptor. We monitor the digestion of our HA-FRET probe with different concentrations of HA-ase in synthetic urine via fluorescence emission. The extent to which FRET is released depends on the concentration of HA-ase. Our fluorescence intensity results are also supported with time resolved fluorescence decay data. This assay can be used to develop a non-invasive technique for the detection of bladder and/or prostate cancer progression.
Rahul
R
Chib
Sangram
S
Raut
Rafal
R
Fudala
Aaron
A
Chang
Mark
M
Mummert
Ryan
R
Rich
Zygmunt
Z
Gryczynski
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA. ignacy.gryczynski@unthsc.edu.
Ignacy
I
Gryczynski
D014408:Biomarkers, Tumor / Q000737:chemistry / Q000652:urine*; D019793:Fluorescein / Q000737:chemistry; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000652:urine*; D008297:Male; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000652:urine*; D012235:Rhodamines / Q000737:chemistry; D001743:Urinary Bladder / Q000737:chemistry; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000652:urine*; D014556:Urine / Q000737:chemistry*
D014408:Biomarkers, Tumor; D012235:Rhodamines; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D019793:Fluorescein