Evaluation of IgG anti-toxoplasma avidity and polymerase chain reaction in the postnatal diagnosis of congenital toxoplasmosis.
Confirmatory tests for congenital toxoplasmosis were evaluated in 23 infected and 31 uninfected newborns. Conventional polymerase chain reaction was better than real-time polymerase chain reaction, but did not identify additional cases. Avidity tests added 2 new cases that were not identified by other criteria. Overall sensitivity was 82.6%. Avidity assay, but not polymerase chain reaction, increased the sensitivity of confirmatory assays in congenital toxoplasmosis.
Grupo de Estudio en Parasitología Molecular, Centro de Investigaciones Biomédicas, Universidad del Quindio, Armenia (Q), Colombia.
Elizabeth
E
Torres
Raul
R
Rivera
Nestor
N
Cardona
Victor
V
Sanchez
Fabiana
F
Lora
Jorge Enrique
JE
Gómez-Marín
D000913:Antibodies, Protozoan / Q000097:blood*; D000915:Antibody Affinity; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G