Antifungal activities of metabolites produced by a termite-associated Streptomyces canus BYB02.
Two main antifungal metabolites resistomycin and tetracenomycin D were isolated and purified from a termite-associated  Streptomyces canus  BYB02 by column chromatography. The structures of isolated compounds were determined on the basis of extensive spectroscopic analysis. Resistomycin possessed strong activities against mycelial growth of  Valsa mali  (IC(50) = 1.1 μg/mL) and  Magnaporthe grisea  (IC(50) = 3.8 μg/mL), which were comparable to those of referenced cycloheximide, with IC(50) values of 2.3 and 0.3 μg/mL, respectively. A further spore germination test showed that resistomycin exhibited potent reduction in spore germination for  M. grisea , with an IC(50) value of 5.55 μg/mL. Finally, the in vivo antifungal activity experiment showed that resistomycin possessed significant preventive efficacy against rice blast, which was more potent than that of referenced carbendazim, with control efficacies of 66.8 and 58.7%, respectively. The present results suggest that resistomycin has potential to be used as a fungicide.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, People's Republic of China. ylzhang@zjnu.cn
Ying-lao
YL
Zhang
Shuai
S
Li
Dong-hua
DH
Jiang
Li-chun
LC
Kong
Ping-hua
PH
Zhang
Jia-dong
JD
Xu
D000818:Animals; D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development; D001580:Benzopyrenes / Q000302:isolation & purification / Q000494:pharmacology; D003513:Cycloheximide / Q000302:isolation & purification / Q000494:pharmacology; D005285:Fermentation; D005659:Fungicides, Industrial / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D020049:Isoptera / Q000382:microbiology*; D020082:Magnaporthe / Q000187:drug effects / Q000254:growth & development; D012275:Oryza; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development; D013302:Streptomyces / Q000378:metabolism*
D001580:Benzopyrenes; D005659:Fungicides, Industrial; C018379:resistomycin; D003513:Cycloheximide