Characterization of a heavy metal translocating P-type ATPase gene from an environmental heavy metal resistance Enterobacter sp. isolate.
Heavy metals are common contaminants found in polluted areas. We have identified a heavy metal translocating P-type ATPase gene (hmtp) via fosmid library and in vitro transposon mutagenesis from an Enterobacter sp. isolate. This gene is believed to participate in the bacterium's heavy metal resistance traits. The complete gene was identified, cloned, and expressed in a suitable Escherichia coli host cell. E. coli W3110, RW3110 (zntA::Km), GG48 (ΔzitB::Cm zntA::Km), and GG51 (ΔzitB::Cm) were used to study the possible effects of this gene for heavy metal (cadmium and zinc in particular) resistance. Among the E. coli strains tested, RW3110 and GG48 showed more sensitivity to cadmium and zinc compared to the wild-type E. coli W3110 and strain GG51. Therefore, strains RW3110 and GG48 were chosen for the reference hosts for further evaluation of the gene's effect. The results showed that expression of this heavy metal translocating P-type ATPase gene could increase the ability for zinc and cadmium resistance in the tested microorganisms.
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Chung-Li, Taiwan. ccchien@saturn.yzu.edu.tw
Chih-Ching
CC
Chien
Chia-Hsuan
CH
Huang
Yi-Wei
YW
Lin
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004754:Enterobacter / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D008969:Molecular Sequence Data; D015032:Zinc / Q000378:metabolism / Q000633:toxicity*
D004785:Environmental Pollutants; D002104:Cadmium; D000251:Adenosine Triphosphatases; D015032:Zinc