Nano-encrypted Morse code: a versatile approach to programmable and reversible nanoscale assembly and disassembly.
While much work has been devoted to nanoscale assembly of functional materials, selective reversible assembly of components in the nanoscale pattern at selective sites has received much less attention. Exerting such a reversible control of the assembly process will make it possible to fine-tune the functional properties of the assembly and to realize more complex designs. Herein, by taking advantage of different binding affinities of biotin and desthiobiotin toward streptavidin, we demonstrate selective and reversible decoration of DNA origami tiles with streptavidin, including revealing an encrypted Morse code "NANO" and reversible exchange of uppercase letter "I" with lowercase "i". The yields of the conjugations are high (>90%), and the process is reversible. We expect this versatile conjugation technique to be widely applicable with different nanomaterials and templates.
Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Ngo Yin
NY
Wong
Hang
H
Xing
Li Huey
LH
Tan
Yi
Y
Lu
D004247:DNA / Q000737:chemistry; D036103:Nanotechnology; D019809:Streptavidin / Q000737:chemistry
D004247:DNA; D019809:Streptavidin