Serum from hepatectomized rats induces the differentiation of adipose             tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression             of hepatocyte growth factor and interleukin-6 in vitro.
Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are an attractive             alternative for clinical application due to their minimally invasive accessibility             and availability in the body. However, the hepatic differentiation efficiency             of AT-MSCs is insufﬁcient for therapeutic application and the role of extrahepatic             stem cells in liver regeneration remains poorly understood. This study was designed             to investigate the effects of serum from rats subjected to 70% partial hepatectomy             (PH) on the differentiation ability of rat AT-MSCs in vitro, and to explore the             potential role of AT-MSCs in vivo following PH injury. Results showed that AT-MSCs             treated with serum collected from rats 24 h after 70% PH differen-tiated into             hepatocyte-like cells, resembled hepatocyte-like cells with round or polygonal             shape, expressed α-fetoprotein, secreted albumin, synthesized urea and acquired             cytochrome P450 type 3A4 enzyme activity, and upregulated the expression of interleukin             (IL)-6 and hepatocyte growth factor (HGF) transiently in vitro, although the hepatic             differentiation efficiency was extremely low. AT-MSC transplantation after 70%             PH ameliorated liver injury and promoted liver regeneration, but did not increase             the serum levels of IL-6 and HGF in vivo. This result suggests that the therapeutic             effect of AT-MSCs in vivo after 24 h of 70% PH does not increase IL-6 and HGF             expression.
Department of General Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, PR China.
Jiangyang
J
Sun
Yufeng
Y
Yuan
Haiquan
H
Qin
Chengcheng
C
Ying
Weijun
W
Liu
Jin
J
Zhang
Yueming
Y
He
Zhisu
Z
Liu
D000273:Adipose Tissue / Q000166:cytology; D000418:Albumins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis*; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology; D015850:Interleukin-6 / Q000096:biosynthesis*; D008115:Liver Regeneration; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D015854:Up-Regulation; D014508:Urea / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000418:Albumins; D015850:Interleukin-6; D000509:alpha-Fetoproteins; D017228:Hepatocyte Growth Factor; D014508:Urea; D003577:Cytochrome P-450 Enzyme System; C465260:Cyp3a2 protein, rat; D051544:Cytochrome P-450 CYP3A