Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea.
Seung Woo
SW
Cho
Sojung
S
Kim
Jong Min
JM
Kim
Jin-Soo
JS
Kim
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D064113:CRISPR-Cas Systems; D053837:DNA Cleavage; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D015894:Genome, Human; D006649:Histocompatibility Antigens / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000235:genetics; D016335:Zinc Fingers; D058727:RNA, Small Untranslated
D001426:Bacterial Proteins; C489165:C4BPB protein, human; D004274:DNA, Recombinant; D006649:Histocompatibility Antigens; D008164:Luminescent Proteins; D019713:Receptors, CCR5; D004720:Endonucleases; D058727:RNA, Small Untranslated