Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis.
A novel implementation of in situ protein digestion supported by a graphene oxide-immobilized enzyme reactor (GO-IMER) in the MALDI imaging mass spectrometry (IMS) workflow is reported, which enables the simultaneous diagnostic identity and distribution attributes of the proteome on tissue.
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, China.
Jing
J
Jiao
Aizhu
A
Miao
Xinyan
X
Zhang
Yan
Y
Cai
Yi
Y
Lu
Ying
Y
Zhang
Haojie
H
Lu
D000595:Amino Acid Sequence; D000818:Animals; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005136:Eye Proteins / Q000032:analysis / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism
D004800:Enzymes, Immobilized; D005136:Eye Proteins; D011506:Proteins; D006108:Graphite; D014357:Trypsin