Down-regulation of cellular protein heme oxygenase 1 inhibits proliferation of classical swine fever virus in PK-15 cells.
Heme oxygenase 1 (HO-1) is an inducible enzyme that exerts potent antioxidant and anti-inflammatory effects, which also plays a critical role in host defenses against microbial, and particularly viral, infections. In our previous study, up-regulation of HO-1 was observed in peripheral blood leukocytes (PBLs) by genomic expression profiling, following infection of pigs with virulent classical swine fever virus (CSFV), the causative agent of a highly contagious disease threatening global pig industry (Shi et al., 2009). To study the potential involvement of HO-1 in CSFV proliferation, the role of its down-regulation in CSFV-infected PK-15 cells was further investigated. Results showed that infection with virulent CSFV strain Shimen significantly up-regulated the expression of HO-1 and that its down-regulation by small interfering RNA (siRNA) could inhibit CSFV proliferation as measured by genomic replication and production of infectious virus. The study revealed the involvement of HO-1 in CSFV proliferation, indicating that HO-1 is a potential target for inhibition of CSFV replication.
Institute of Veterinary Sciences, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, 666 Liuying West Road, Changchun 130122, China.
Zixue
Z
Shi
Jinfu
J
Sun
Huancheng
H
Guo
Zhi
Z
Yang
Zhiyong
Z
Ma
Changchun
C
Tu
D000818:Animals; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000502:physiology*; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000235:genetics; D054884:Host-Pathogen Interactions; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014779:Virus Replication
D034741:RNA, Small Interfering; D051547:Heme Oxygenase-1