An optimal polymerization conditions for poly-human placenta hemoglobin with lower mean molecular weight.
OBJECTIVE
To reduce the mean molecular weight [Formula: see text] and to increase the effective polymerization ratio (REff) of polymerized human placenta hemoglobin (PolyPHb).


METHODS
Three factors of GDA-PolyPHb process such as the approach of feeding GDA (FGDA), hemoglobin concentration ([Hb]) and the molar ratio of GDA, and hemoglobin(RGDA:Hb) were investigated. Finally, the expansion experiments were conducted with optimal conditions.


RESULTS
The data showed that the HBOCs with the REff of 67.35% and lower [Formula: see text] of 162.70 kDa were prepared by optimal conditions.


CONCLUSIONS
Compared to original process, the optimal process greatly decreased the [Formula: see text] and increased the REff.
School of Pharmaceutical Science & Technology, Tianjin University , Tianjin , P. R. China.
Wentao
W
Zhou
Lanzhen
L
Zhao
Jinfeng
J
Wang
Shen
S
Li
Gang
G
Chen
Jiaxin
J
Liu
Chengmin
C
Yang
D001803:Blood Transfusion; D002474:Cell-Free System; D000529:Complementary Therapies; D005260:Female; D005976:Glutaral / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D010920:Placenta / Q000378:metabolism; D058105:Polymerization; D011247:Pregnancy
D006454:Hemoglobins; D005976:Glutaral