Differences of calcium binding proteins immunoreactivities in the young hippocampal CA1 region from the adult following transient ischemic damage.
It has been reported that the young were much more resistant to transient cerebral ischemia than in the adult. In the present study, we examined that about 90% of CA1 pyramidal cells in the adult gerbil hippocampus died at 4days after ischemia-reperfusion; however, in the young hippocampus, about 56% of them died at 7days after ischemia-reperfusion. We compared immunoreactivities and levels of calcium binding proteins (CBPs), such as calbindin 28k (CB-D28k), calretinin (CR) and parvalbumin (PV). The immunoreactivities and protein levels of all the CBPs in the young sham were higher than those in the adult sham. In the adult, the immunoreactivities and protein levels of all the CBPs were markedly decreased at 4days after ischemia-reperfusion, however, in the young, they were apparently maintained. At 7days after ischemia-reperfusion, they were decreased in the young, however, they were much higher than those in the adult. In brief, the immunoreactivities and levels of CBPs were not decreased in the ischemic CA1 region of the young 4days after transient cerebral ischemia. This finding indicates that the longer maintenance of CBPs may contribute to a less and more delayed neuronal death/damage in the young.
Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, Seoul, 140-743 South Korea.
Young Joo
YJ
Lee
Bing Chun
BC
Yan
Joon Ha
JH
Park
Ji Hyeon
JH
Ahn
In Hye
IH
Kim
Jae-Chul
JC
Lee
Hui Young
HY
Lee
Young-Myeong
YM
Kim
Moo-Ho
MH
Won
Jun Hwi
JH
Cho
D000367:Age Factors; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000276:immunology* / Q000378:metabolism*; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D011485:Protein Binding / Q000276:immunology
D002135:Calcium-Binding Proteins