Simultaneous single-particle superlocalization and rotational tracking.
Superlocalization of single molecules and nanoparticles has become an essential procedure to bring new insights into nanoscale structures and dynamics of biological systems. In the present study, superlocalization is combined with the newly introduced differential interference contrast (DIC) microscopy-based single-particle orientation and rotational tracking. The new technique overcomes the difficulty in localization of the antisymmetric DIC point spread function by using a dual-modality microscope configuration for simultaneous rotational tracking and localization of single gold nanorods with nanometer-scale precision. The new imaging setup has been applied to study the steric hindrance induced by relatively large cargos in the microtubule gliding assay and to track nanocargos in the crowded cellular environment. This technique has great potential in the study of biological processes where both localization and rotational information are required.
Ames Laboratory, U.S. Department of Energy, and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
Yan
Y
Gu
Gufeng
G
Wang
Ning
N
Fang
D000818:Animals; D002470:Cell Survival; D004705:Endocytosis; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D008853:Microscopy; D055620:Optical Phenomena; D016716:PC12 Cells; D010316:Particle Size; D051381:Rats; D012399:Rotation; D013997:Time Factors
D006046:Gold