Aryl azide photochemistry in defined protein environments.
A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.
School of Chemistry, Cardiff University, Cardiff CF10 3AT United Kingdom.
Josephine L
JL
Morris
Samuel C
SC
Reddington
Damien M
DM
Murphy
D Dafydd
DD
Jones
James A
JA
Platts
Eric M
EM
Tippmann
D001386:Azides / Q000737:chemistry*; D017122:Bacteriophage T4 / Q000201:enzymology / Q000235:genetics; D004578:Electron Spin Resonance Spectroscopy; D049452:Green Fluorescent Proteins / Q000737:chemistry; D007097:Imines / Q000737:chemistry; D015394:Molecular Structure; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D010777:Photochemistry; D010782:Photolysis; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D001386:Azides; D007097:Imines; D014764:Viral Proteins; C068293:gene 5 protein, Enterobacteria phage T4; D049452:Green Fluorescent Proteins; C017621:phenylnitrene; C027162:4-azidophenylalanine; D010649:Phenylalanine