Multiplexed detection of protein markers with silicon nanowire FET and sol-gel matrix.
A new modified top-down based fabrication method has been developed to obtain highly ordered silicon nanowire (SiNW) arrays. With this method, we could produce as many as 500 chips (size dependable) in an 8 inch wafer. The immobilization of multiple proteins on each chip was performed by spotting sol/gel materials encapsulating antibodies on the different regions. The most commonly used protein markers in clinics, such as C-reactive protein (CRP) and prostate specific antigen (PSA), were systematically entrapped in sol-gel materials and used for multiplexed testing. Upon formation, the electrical signal of different concentrations of CRP and PSA could be simultaneously determined in the range of 0.12 ∼ 10 ng/mL and 0.18 ∼ 8.87 ng/mL, respectively.
Medical IT research center, Korea Electronics Technology Institute, 463-816, Korea. mhlee@keti.re.kr
Min-Ho
MH
Lee
Kooknyung
K
Lee
Suk Won
SW
Jung
D055910:Antibodies, Immobilized; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D002097:C-Reactive Protein / Q000032:analysis; D004867:Equipment Design; D006801:Humans; D008297:Male; D053770:Nanowires; D044367:Phase Transition; D017430:Prostate-Specific Antigen / Q000032:analysis; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*; D012825:Silicon; D014173:Transistors, Electronic
D055910:Antibodies, Immobilized; D015415:Biomarkers; D011506:Proteins; D002097:C-Reactive Protein; D017430:Prostate-Specific Antigen; D012825:Silicon