Molecular cartography in acute Chlamydia pneumoniae infections--a non-targeted metabolomics approach.
Infections with Chlamydia pneumoniae cause several respiratory diseases, such as community-acquired pneumonia, bronchitis or sinusitis. Here, we present an integrated non-targeted metabolomics analysis applying ultra-high-resolution mass spectrometry and ultra-performance liquid chromatography mass spectrometry to determine metabolite alterations in C. pneumoniae-infected HEp-2 cells. Most important permutations are elaborated using uni- and multivariate statistical analysis, logD retention time regression and mass defect-based network analysis. Classes of metabolites showing high variations upon infection are lipids, carbohydrates and amino acids. Moreover, we observed several non-annotated compounds as predominantly abundant after infection, which are promising biomarker candidates for drug-target and diagnostic research.
Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany.
Constanze
C
Müller
Inga
I
Dietz
Dimitrios
D
Tziotis
Franco
F
Moritz
Jan
J
Rupp
Philippe
P
Schmitt-Kopplin
D015415:Biomarkers; D002623:Chemistry Techniques, Analytical; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D002853:Chromatography, Liquid / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*
D015415:Biomarkers