Non-hazardous anticancerous and antibacterial colloidal 'green' silver nanoparticles.
Poly(ethylene glycol) stabilized colloidal silver nanoparticles were prepared using the reductive potency of the aqueous extract of Thuja occidentalis leaves under ambient conditions. The nanoparticles were well dispersed within a narrow size spectrum (7-14 nm) and displayed characteristic surface plasmon resonance peak at around 420 nm and Bragg's reflection planes of fcc structure. MTT assay revealed the dose-dependent cytocompatibility and toxicity of the nanoparticles with the L929 normal cell line. On the other hand, the antiproliferative action of the nanoparticles was evaluated on HeLa cell (cancerous cells) line. Fluorescence and phase contrast microscopic imaging indicated the appearance of multinucleate stages with aggregation and nuclear membrane disruption of the HeLa cells post treatment with the nanoparticles. The interaction at the prokaryotic level was also assessed via differential antibacterial efficacy against Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 40). Under these perspectives, it is also necessary to observe the environmental impact of the prepared silver nanoparticles. Hence, the dose dependent toxicity of silver nanoparticles was evaluated upon the earthworm species Eisenia fetida. Neither the survival nor the reproduction was affected by the addition of silver nanoparticles up to 1000 ppm. Thus these 'green' silver nanoparticles have promising potential as future materials.
Advanced Polymer and Nanomaterial Laboratory, Department of Chemical Sciences, Tezpur University, Assam, India.
Shaswat
S
Barua
Rocktotpal
R
Konwarh
Satya Sundar
SS
Bhattacharya
Pallabi
P
Das
K Sanjana P
KS
Devi
Tapas K
TK
Maiti
Manabendra
M
Mandal
Niranjan
N
Karak
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008858:Microscopy, Phase-Contrast; D009835:Oligochaeta / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012098:Reproduction / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects*; D020349:Surface Plasmon Resonance; D015996:Survival Rate; D029783:Thuja / Q000737:chemistry
D010936:Plant Extracts; D012834:Silver