The evolution and pathogenic mechanisms of the rice sheath blight pathogen.
Rhizoctonia solani is a major fungal pathogen of rice (Oryza sativa L.) that causes great yield losses in all rice-growing regions of the world. Here we report the draft genome sequence of the rice sheath blight disease pathogen, R. solani AG1 IA, assembled using next-generation Illumina Genome Analyser sequencing technologies. The genome encodes a large and diverse set of secreted proteins, enzymes of primary and secondary metabolism, carbohydrate-active enzymes, and transporters, which probably reflect an exclusive necrotrophic lifestyle. We find few repetitive elements, a closer relationship to Agaricomycotina among Basidiomycetes, and expand protein domains and families. Among the 25 candidate pathogen effectors identified according to their functionality and evolution, we validate 3 that trigger crop defence responses; hence we reveal the exclusive expression patterns of the pathogenic determinants during host infection.
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu 611130, China. apzh0602@gmail.com
Aiping
A
Zheng
Runmao
R
Lin
Danhua
D
Zhang
Peigang
P
Qin
Lizhi
L
Xu
Peng
P
Ai
Lei
L
Ding
Yanran
Y
Wang
Yao
Y
Chen
Yao
Y
Liu
Zhigang
Z
Sun
Haitao
H
Feng
Xiaoxing
X
Liang
Rongtao
R
Fu
Changqing
C
Tang
Qiao
Q
Li
Jing
J
Zhang
Zelin
Z
Xie
Qiming
Q
Deng
Shuangcheng
S
Li
Shiquan
S
Wang
Jun
J
Zhu
Lingxia
L
Wang
Huainian
H
Liu
Ping
P
Li
D005075:Biological Evolution; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015203:Reproducibility of Results; D012232:Rhizoctonia / Q000235:genetics* / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics; D013025:Glycine max / Q000382:microbiology; D059467:Transcriptome / Q000235:genetics; D037521:Virulence Factors / Q000378:metabolism; D003313:Zea mays / Q000382:microbiology
D005656:Fungal Proteins; D037521:Virulence Factors