Gold nanoparticle clusters in quasinematic layers of liquid-crystalline dispersion particles of double-stranded nucleic acids.
The interaction between gold nanoparticles and particles of cholesteric liquid-crystalline dispersions formed by double-stranded DNA and poly(I)×poly(C) molecules is considered. It is shown that small-sized (~ 2 nm) gold nanoparticles induce two different structural processes. First, they facilitate the reorganization of the spatial cholesteric structure of the particles into a nematic one. This process is accompanied by a fast decrease in the amplitude of an abnormal band in the CD spectrum. Second, they induce cluster formation in a "free space" between neighboring nucleic acid molecules fixed in the structure of the quasinematic layers of liquid-crystalline particles. This process is accompanied by slow development of the surface plasmon resonance band in the visible region of the absorption spectrum. Various factors influencing these processes are outlined. Some assumptions concerning the possible mechanism(s) of fixation of gold nanoparticles between the neighboring double-stranded nucleic acid molecules in quasinematic layers are formulated.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str., 32, Moscow, Russia, 119991.
Yu M
YM
Yevdokimov
V I
VI
Salyanov
E I
EI
Katz
S G
SG
Skuridin
DNA; abnormal optical activity; absorption spectroscopy; circular dichroism; cytotoxicity of nanoparticles; gold nanoparticles; liquid-crystalline dispersions of nucleic acids; poly(I)×poly(C); structure of biopolymer lyotropic liquid crystals; surface plasmon resonance