Proteomic and histological analyses of endosperm development in Cyclamen persicum as a basis for optimization of somatic embryogenesis.
The endosperm plays an important role for the development of zygotic embryos, while somatic embryos lack a seed coat and endosperm and often show physiological disorders. This study aims at elucidating the cellular and physiological processes within the endosperm of the ornamental species Cyclamen persicum Mill. Histological analyses were performed from 0 to 11 weeks after pollination (WAP). At 3WAP, a syncytium was clearly visible with a globular zygotic embryo. From 4WAP, cellularization of the endosperm, at 5WAP a small torpedo shaped embryo, and from 7WAP cell expansion was observed. By 11WAP the endosperm appeared fully differentiated. Total soluble proteins were extracted from the endosperm at 4, 5, 7, 9 and 11WAP and resolved using two dimensional isoelectric focussing/sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D IEF/SDS-PAGE). A shift from high-molecular-mass proteins to low-molecular-mass proteins during endosperm development was observed. A total of 1137proteinspots/gel were detected in the three protein fractions extracted at 7, 9 and 11WAP. Mass spectrometry analysis of the 48 predominant protein spots in endosperm at 7, 9 and 11WAP resulted in the identification of 62 proteins, ten of which were described for the first time in Cyclamen. Additionally, 186 proteins were identified using the C. persicum embryo proteome reference map. Proteins involved in abscisic acid signalling and oxidative stress responsive proteins were found to be important for seed development in Cyclamen. The new insights into endosperm physiology including storage compounds are discussed.
Institute of Plant Genetics, Leibniz Universitaet Hannover, Herrenhaeuser Str. 2, D-30419 Hannover, Germany.
Jenniffer Wamaitha
JW
Mwangi
Christina
C
Rode
Frank
F
Colditz
Christin
C
Haase
Hans-Peter
HP
Braun
Traud
T
Winkelmann
D000040:Abscisic Acid / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division; D031824:Cyclamen / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D056625:Endosperm / Q000166:cytology* / Q000254:growth & development*; D007525:Isoelectric Focusing / Q000379:methods; D013058:Mass Spectrometry; D008970:Molecular Weight; D018384:Oxidative Stress; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D054817:Pollination; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D055314:Seed Storage Proteins / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
D018797:Cell Cycle Proteins; D020543:Proteome; D055314:Seed Storage Proteins; D000040:Abscisic Acid