Proteomic analysis of Spirogyra varians mutant with high starch content and growth rate induced by gamma irradiation.
This study was conducted to develop a high-efficiency strain of Spirogyra varians for the production of biomass by radiation breeding. The characteristics of wild-type and mutant S. varians were analyzed through phenomenological and proteomic observations. The results of our phenomenological observations of the S. varians mutant demonstrated increases in growth rate and content of chlorophyll a, b, and a + b; in particular, a significant threefold increase was observed in starch accumulation. Proteomic analysis to investigate the differences in expression between wild-type and mutant proteins identified 18 proteins with significantly different expressions. From the literature review, it was confirmed that the up-regulated proteins were mainly involved in photosynthesis, carbohydrate biosynthesis, and energy metabolism. These results suggest the possibility of algae development by radiation breeding for the production of biofuel.
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, South Korea.
Minchul
M
Yoon
Jong-il
JI
Choi
Gwang Hoon
GH
Kim
Dong-Ho
DH
Kim
Don-Hee
DH
Park
D002734:Chlorophyll / Q000096:biosynthesis / Q000235:genetics; D005720:Gamma Rays; D009154:Mutation; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D020543:Proteome / Q000096:biosynthesis / Q000235:genetics; D040901:Proteomics / Q000379:methods; D058131:Spirogyra / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013213:Starch / Q000096:biosynthesis / Q000235:genetics
D010940:Plant Proteins; D020543:Proteome; D002734:Chlorophyll; D013213:Starch