Small-molecule-mediated axonal branching in Caenorhabditis elegans.
An in vivo system for monitoring small-molecule-mediated neuronal branching has been developed by using C. elegans. Growth-promoting compounds can be detected by visual inspection of GFPlabeled cholinergic neurons, as axonal branching occurs following treatment with neurotrophic agents. Investigation of the structure-activity relationship of the neurotrophic natural product clovanemagnolol (1) led us to a comparable chemically edited derivative.
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78701, USA.
Katherine
K
Zlotkowski
Jon
J
Pierce-Shimomura
Dionicio
D
Siegel
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D010636:Phenols; D012717:Sesquiterpenes; D054852:Small Molecule Libraries; C549996:clovanemagnolol; D049452:Green Fluorescent Proteins