Genetically encoded copper(I) reporters with improved response for use in imaging.
Copper represents one of the most important biological metal ions due to its role as a catalytic cofactor in a multitude of proteins. However, an excess of copper is highly toxic. Thus, copper is heavily regulated, and copper homeostasis is controlled by many metalloregulatory proteins in various organisms. Here we report a genetically encoded copper(I) probe capable of monitoring copper fluctuations inside living cells. We insert the copper regulatory protein Ace1 into a yellow fluorescent protein, which selectively binds copper(I) and generates improved copper(I) probes.
Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Jun
J
Liu
Jason
J
Karpus
Seraphine V
SV
Wegner
Peng R
PR
Chen
Chuan
C
He
D002384:Catalysis; D002469:Cell Separation; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006706:Homeostasis; D006801:Humans; D015335:Molecular Probes; D013050:Spectrometry, Fluorescence
D015335:Molecular Probes; D003300:Copper