[Blockage of Th17 cells differentiation exacerbated mouse acute intestine graft-versus-host disease following allogeneic bone marrow transplantation].
OBJECTIVE
To study the role of Th17 cells in acute intestine graft-versus-host disease following allogenetic bone marrow transplantation(allo-BMT).


METHODS
Mice were split randomly into five groups: normal control, irradiated, allo-BMT, allo-BMT + DMSO and allo-BMT + Halofuginone (HF) groups. HF was given intraperitoneally at a dose of 5 µg per mouse from -1 d to 10 d after allo-BMT. aGVHD symptoms were followed-up to perform clinical and pathogenic scores. The levels of Th1/Th17, interleukin-17 and interferon-γ were measured by flow cytometry at day 7 d. mRNA expressions of T-bet, RORγT, CXCL9, CXCL10, CXCL11 and CCL20 in intestine were evaluated by real-time PCR.


RESULTS
Intestinal damages in allo-BMT-HF mice was more serious than in normal control and allo-BMT groups at day 14 after transplantation. At day 7, Th17 ratio in allo-BMT + HF group was significantly lower than in allo-BMT group. IL-17A was not detected, but Th1 ratio was higher in allo-BMT + HF. There was a similar increment in the relative expressions of T-bet in both allo-BMT and allo-BMT + HF groups. Expressions of CXCL9 and CXCL10 elevated in allo-BMT + HF group, which were significantly higher than those in allo-BMT group (P < 0.01). CCL20 expression significantly increased in allo-BMT group, but it was not detected in allo-BMT + HF group.


CONCLUSIONS
Blockage of th17 cells differentiation exacerbated acute intestine graft versus-host disease.
Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Hu-jun
HJ
Li
Hai
H
Cheng
Bin
B
Pan
Ling-yu
LY
Zeng
Kai-lin
KL
Xu
D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002454:Cell Differentiation; D005260:Female; D006086:Graft vs Host Disease / Q000473:pathology*; D007410:Intestinal Diseases / Q000473:pathology*; D007422:Intestines / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D058504:Th17 Cells / Q000166:cytology*; D014184:Transplantation, Homologous