Dys-psychological Stress Effect on Expressions of P53 and NFκBp65 in Human Ovarian Carcinoma In Vivo.
OBJECTIVE
To investigate the dys-psychological stress effect on the growth of subcutaneous xenotransplanted tumor in nude mice bearing human epithelium ovarian carcinoma, and the influence on P53 and NFκBp65 expressions.


METHODS
The subcutaneous tumor xenografts were established by implanting human epithelium ovarian carcinoma tissues into nude mice and the dys-psychological stress model was established with restraint. The mice were randomized into the following four treatment groups with each group six mice respectively: tumor group (group A), normal saline intraperitoneal injection; tumor with stress group (group B), normal saline intraperitoneal injection; tumor therapy group (group C), cisplatin intraperitoneal injection; and tumor therapy with stress group (group D), cisplatin intraperitoneal injection. The expressions of P53 and NFκBp65 in tumor tissues were determined by Western blotting.


RESULTS
The expressions of P53 and NFκBp65 in each restraint group were enhanced compared with the control groups (P<0.05).


CONCLUSIONS
The dys-psychological stress may induce the high expressions of P53 and NFκBp65 proteins and further promote tumor growth.
Department of Gynecology, Aviation General Hospital, Beijing 100012, China.
Li-Qun
LQ
Yu
Guo-Lan
GL
Gao
Fun-Jun
FJ
Liu
Qiong-Jing
QJ
Zeng
Constraint stress; Oncoprotein; Ovarian cancer animal model