Quantification of ovarian cancer markers with integrated microfluidic concentration gradient and imaging nanohole surface plasmon resonance.
Nanohole array-based biosensors integrated with a microfluidic concentration gradient generator were used for imaging detection and quantification of ovarian cancer markers. Calibration curves based on controlled concentrations of the analyte were created using a microfluidic stepped diffusive mixing scheme. Quantification of samples with unknown concentration of analyte was achieved by image-intensity comparison with the calibration curves. The biosensors were first used to detect the immobilization of ovarian cancer marker antibodies, and subsequently applied for the quantification of the ovarian cancer marker r-PAX8 (with a limit of detection of about 5 nM and a dynamic range from 0.25 to 9.0 μg.mL(-1)). The proposed biosensor demonstrated the ability of self-generating calibration curves on-chip in an integrated microfluidic platform, representing a further step towards the development of comprehensive lab-on-chip biomedical diagnostics based on nanohole array technology.
ETH Zurich, Department of Biosystems Science and Engineering, Mattenstrasse 26, Basel, 4058, Switzerland. carlos.escobedo@bsse.ethz.ch
Carlos
C
Escobedo
Yu-Wei
YW
Chou
Mohammad
M
Rahman
Xiaobo
X
Duan
Reuven
R
Gordon
David
D
Sinton
Alexandre G
AG
Brolo
Jacqueline
J
Ferreira
D055910:Antibodies, Immobilized / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis*; D004867:Equipment Design; D005260:Female; D006801:Humans; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D000072636:PAX8 Transcription Factor; C083325:PAX8 protein, human; D051761:Paired Box Transcription Factors