Maintenance of "stem cell" features of cartilage cell sub-populations during in vitro propagation.
BACKGROUND
The discovery of mesenchymal stem cells (MSCs) or MSC-like cells in cartilage tissue does not tie in well with the established view that MSCs derive from a perivascular niche. The presence of MSCs may raise concerns about specificity and application safety, particularly in terms of the regulatory site. The aim of the present study was to investigate the benefits or possible risks of the MSC-like properties of cells isolated from cartilage in the context of autologous chondrocyte implantation.


METHODS
Chondrocytic cells were isolated from cartilage or intervertebral disc tissue. Flow cytometry was used to analyze the expression of cell surface antigens. MSC-like cells were either enriched or depleted by means of magnetic cell sorting (MACS) involving the monoclonal antibodies W5C5/SUSD2 and W8B2/MSCA-1. We addressed the issues of prolonged expansion of such cells as well as the influence of culture medium as a trigger for selecting a single cell type. Established protocols were used to study in vitro differentiation. In addition to histological and biochemical assessment, the acquired phenotypes were also evaluated on the mRNA transcript level.


RESULTS
In the studied cells, we found strongly analogous expression of antigens typically expressed on MSCs, including CD49e, CD73, CD90, CD105, CD140b and CD166. The expression of W5C5 and W8B2 antigens in cartilage cell sub-populations did not correlate with multi-potency. We demonstrated that a chondroid precursor, but not a bona fide multipotent mesenchymal, cell type can be obtained under established in vitro culture conditions. The culture media used for expansion influenced the cell phenotype.


CONCLUSIONS
The risk of adverse adipose or osseous differentiation is not posed by expanded chondrocyte cultures, even after enrichment of putative MSC-like cell populations by MACS. It is possible that this limited "stemness" in chondrocytes, expanded for use in ACI, may instead be beneficial as it allows re-differentiation under appropriate conditions despite prolonged times in culture.
NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr, 55, Reutlingen 72770, Germany. benz@nmi.de
Karin
K
Benz
Claudia
C
Stippich
Christian
C
Freudigmann
Juergen A
JA
Mollenhauer
Wilhelm K
WK
Aicher
D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology; D000941:Antigens / Q000378:metabolism; D002356:Cartilage / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D019902:Chondrocytes / Q000166:cytology*; D000939:Epitopes / Q000737:chemistry; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D010012:Osteogenesis; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
D000941:Antigens; D000939:Epitopes; D012333:RNA, Messenger