Increased levels of serum and gingival crevicular fluid monocyte chemoattractant protein-1 in smokers with periodontitis.
BACKGROUND
Smoking alters the host response, including vascular function, neutrophil/monocyte activities, adhesion molecule expression, antibody production, and cytokine and inflammatory mediator release. Monocyte chemoattractant protein-1 (MCP-1) is involved in the activation and recruitment of inflammatory and immune cells to infected sites, thereby mediating a variety of pathophysiologic conditions. Estimation of serum and gingival crevicular fluid (GCF) MCP levels could be a reliable indicator of periodontal disease activity. Hence, the objective of this study is to analyze the serum and GCF MCP-1 levels of smokers and never-smokers with periodontitis and compare them with those in periodontally healthy individuals.


METHODS
A total of 90 participants (30 periodontally healthy individuals, 30 non-smoking individuals with periodontitis, and 30 smokers with periodontitis) formed the study group. Serum and GCF samples were collected, and MCP-1 levels were estimated using enzyme-linked immunosorbent assay.


RESULTS
Mean MCP-1 levels in serum and GCF were found to be highest in smokers with periodontitis, followed by the periodontitis group, and then by the healthy controls. The values were statistically significant (P <0.001).


CONCLUSIONS
It can be concluded that the high levels of both serum and GCF MCP-1 found in smokers could explain the severity of periodontitis in smokers. More longitudinal, prospective studies will help to verify the observations of the present study. Further research in this direction could reveal reliable markers to forecast the progression of periodontitis in high-risk groups.
Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. anil@graduate.hku.hk
Sukumaran
S
Anil
R S
RS
Preethanath
Mohammed
M
Alasqah
Sameer A
SA
Mokeem
Pradeep S
PS
Anand
D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D018932:Chemokine CCL2 / Q000032:analysis / Q000097:blood*; D018450:Disease Progression; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000097:blood / Q000378:metabolism; D010514:Periodontal Pocket / Q000097:blood / Q000378:metabolism; D010518:Periodontitis / Q000097:blood* / Q000145:classification / Q000378:metabolism; D012907:Smoking / Q000097:blood* / Q000378:metabolism
D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2