Bone morphogenic protein-2 (BMP-2) loaded hybrid coating on porous hydroxyapatite scaffolds for bone tissue engineering.
In this study, a silica xerogel-chitosan hybrid is utilized as a coating material to incorporate bone morphogenic protein-2 (BMP-2) on a porous hydroxyapatite (HA) scaffold for bone tissue engineering. BMP-2 is known as a therapeutic agent for improving bone regeneration and repair. Silica xerogel-chitosan hybrids have been used for the delivery of a growth factor as well as osteoconductive coatings. The biological properties of the hybrid coating incorporated with BMP-2 were evaluated in terms of the BMP-2 release behavior, osteoblastic cellular responses and in vivo performance. BMP-2 was continuously released from the hybrid coating layer on the porous HA scaffold for up to 6 weeks. The hybrid coating containing BMP-2 showed significantly enhanced osteoblastic cell responses in comparison with the hybrid coating and HA substrate. Consequently, new bone formation was significantly increased within the hybrid coating containing BMP-2. These results reveal that the hybrid coating containing BMP-2 has the potential to be used as a bone implant, whose osteogenic properties are promoted by the release of BMP-2 in a controlled manner for a prolonged period of time.
Department of Materials Science and Engineering, Seoul National University, Seoul, Korea.
Shin-Hee
SH
Jun
Eun-Jung
EJ
Lee
Tae-Sik
TS
Jang
Hyoun-Ee
HE
Kim
Jun-Hyeog
JH
Jang
Young-Hag
YH
Koh
D000818:Animals; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D017931:DNA Primers; D017886:Durapatite; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014057:Tomography, X-Ray Computed
D055396:Bone Morphogenetic Protein 2; D017931:DNA Primers; D017886:Durapatite