Immunohistochemical evaluation of heat shock protein expression in normal canine nerve and peripheral nerve sheath tumours.
Abnormal expression of heat shock proteins (HSPs) has been observed in many human neoplasms and such expression has prognostic, predictive and therapeutic implications. The aim of this study was to evaluate immunohistochemically the expression of HSP 27, HSP 32 and HSP 90 in normal canine peripheral nerves and in four benign and 15 malignant canine peripheral nerve sheath tumours (PNSTs). In normal nerve, all of the HSPs were detected in axons, epineurial fibroblasts and scattered Schwann cell bodies. Cytoplasmic expression of HSP 27 was more widespread and intense in benign PNSTs compared with malignant PNSTs (P <0.05). Widespread and intense nuclear expression of HSP 32 was also associated with benign tumours (P <0.01), while high HSP 90 immunoreactivity was detected in all tumours, suggesting that HSP 90 might represent a new therapeutic target.
Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, Piazza A Moro 45, 64100 Teramo, University of Teramo, Piazza A Moro 45, 64100 Teramo, Italy.
M
M
Romanucci
I
I
Berardi
A
A
Ciccarelli
L
L
Bongiovanni
D
D
Malatesta
A
A
Oevermann
L
L
Della Salda
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D007150:Immunohistochemistry; D018317:Nerve Sheath Neoplasms / Q000378:metabolism / Q000662:veterinary*; D010525:Peripheral Nerves / Q000378:metabolism
D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins
dog; heat shock protein; immunohistochemistry; peripheral nerve sheath tumour