Antimicrobial and antifungal activities of Lactobacillus curvatus strain isolated from homemade Azerbaijani cheese.
The aims of this study were to characterize inhibitory activity spectra, some probiotic properties and safety of Lactobacillus curvatus A61 for its future application in production of fermented foods. The studied strain was isolated from traditional homemade cheese manufactured in Azerbaijan. The cell-free supernatant of culture of Lb. curvatus A61 inhibited the growth of tested LAB, as well as of Listeria monocytogenes and Bacillus cereus strains. The strain presented antifungal activity and inhibited the growth of Cladosporium and Fusarium ssp. during co-cultivation on agar media. PCR amplification with specific primers revealed the presence of curvacin A encoding gene in Lb. curvatus A61. Bacteriocin produced by the studied strain was heat stable and active in a broad pH range, and in the presence of Triton X-20, Triton X-80, Triton X-100, β-mercaptoethanol, Na-EDTA, SDS and NaCl. The mode of action of bacteriocin against selected indicator strains was found to be bacteriostatic. Lb. curvatus A61 was resistant to physiological concentrations of bile salts and showed high auto-aggregation ability, as well as co-aggregation ability with pathogenic L. monocytogenes strains. It was sensitive to chloramphenicol, penicillin, tetracycline, ciprofloxacin and vancomycin, but resistant to ampicillin and gentamicin.
UR 1268, INRA Biopolymères Interactions Assemblages - Equipe FIP, B.P. 71627, 44316 Nantes Cedex 03, France.
Aynur
A
Ahmadova
Svetoslav Dimitrov
SD
Todorov
Imen
I
Hadji-Sfaxi
Yvan
Y
Choiset
Hanitra
H
Rabesona
Soumaya
S
Messaoudi
Akif
A
Kuliyev
Bernadette Dora Gombossy de Melo
BD
Franco
Jean-Marc
JM
Chobert
Thomas
T
Haertlé
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D000935:Antifungal Agents / Q000378:metabolism*; D001382:Azerbaijan; D001409:Bacillus cereus / Q000187:drug effects; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics; D002611:Cheese / Q000382:microbiology*; D002963:Cladosporium / Q000187:drug effects; D005285:Fermentation; D005670:Fusarium / Q000187:drug effects; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D019936:Probiotics
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001430:Bacteriocins; C071456:sakacin A