Recipient cytotoxic T lymphocyte antigen 4 +49 single-nucleotide polymorphism is not associated with acute rejection after liver transplantation in Chinese population.
OBJECTIVE
Single-nucleotide polymorphisms (SNPs) in Cytotoxic T lymphocyte antigen 4 (CTLA-4) gene have been detected and proved to associate with the incidence of rejection after transplantation. However, previous studies gained inconsistent results about the association between CTLA-4 +49 single-nucleotide polymorphism and susceptibility of allograft rejection. Therefore we sought to clarify whether CTLA-4 +49 SNP influences the incidence of acute rejection after liver transplantation in Chinese population.


METHODS
Genomic DNA from 335 liver transplant recipients was genotyped for CTLA-4 +49 SNP by DNA sequencing. Acute rejection was confirmed by pathologic evidences. The association between CTLA-4 +49 SNP and incidence of acute rejection was then analyzed by dominant, recessive, codominant and overdominant models.


RESULTS
The incidence of acute rejection within the first 3 months was 11.9%. In acute rejectors, the frequency was 45% for G/G, 10% for A/A and 45% for A/G respectively, compared with 47.5% for G/G, 10.8% for A/A and 41.7% for A/G in non-acute rejectors. And no significant difference of allele distribution between these 2 groups was detected.


CONCLUSIONS
This study suggests that CTLA-4 +49 SNP is not associated with acute rejection after liver transplantation in Chinese population.
Division of Hepatobiliary Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou Zhejiang 310003, P.R. China.
Zhijun
Z
Jiang
Ying
Y
Chen
Xiaonin
X
Feng
Haiyang
H
Xie
Lin
L
Zhou
Shusen
S
Zheng
D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D060908:CTLA-4 Antigen / Q000235:genetics* / Q000276:immunology; D005260:Female; D056726:Genetic Association Studies; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016031:Liver Transplantation / Q000276:immunology*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
D060908:CTLA-4 Antigen
Cytotoxic T lymphocyte antigen 4; Single Nucleotide Polymorphism.; liver transplantation