Niche-less maintenance of HSCs by 2i.
Maintenance of hematopoietic stem cells (HSCs) in vitro has been believed to be difficult due to a lack of complete understanding of HSC quiescence maintained by the niche. Recent evidence suggests that in vitro maintenance of human and mouse long-term HSCs (LT-HSCs) is possible through dual inhibition (2i) of both GSK-3 and mTOR in the absence of cytokines, serum, or feeder cells.
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Shigeo
S
Masuda
Mo
M
Li
Juan Carlos
JC
Izpisua Belmonte
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006706:Homeostasis / Q000502:physiology*; D006801:Humans; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000502:physiology*
D058570:TOR Serine-Threonine Kinases