[Changing of the expression of VEGF genes encoded important regulators of angiogenesis and neurogenesis under hypoxic and ischemic conditions].
The study of cellular and molecular processes that underlie the regeneration of nerve tissue is one of the most important problems of modern neuroscience. The solution of this problem is associated with the development of the new methods for the treatment of ischemic stroke. This paper investigated one of the key regulators of angio- and neurogenesis--vascular endothelial growth factor (VEGF). We stadied changes in the expression of VEGF genes under ischemia and hypoxic conditions in glioma cell lines U87 and its dependence on the ERN1 gene function. We found that the expression of VEGF genes in glioma cell lines U87 is significantly changed under hypoxia and ischemic conditions. Its expression depends upon the blockade of the ERN1 gene function.
O V
OV
Hubenia
D O
DO
Minchenko
O H
OH
Minchenko
N K
NK
Murashko
D018929:Cell Culture Techniques; D015687:Cell Hypoxia / Q000235:genetics; D045744:Cell Line, Tumor; D003470:Culture Media; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005910:Glioma / Q000473:pathology; D005947:Glucose / Q000172:deficiency; D005973:Glutamine / Q000172:deficiency; D006801:Humans; D007511:Ischemia / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000235:genetics*; D055495:Neurogenesis / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D003470:Culture Media; D008565:Membrane Proteins; D042461:Vascular Endothelial Growth Factor A; D005973:Glutamine; C403982:ERN2 protein, human; D017346:Protein Serine-Threonine Kinases; D004722:Endoribonucleases; D005947:Glucose; D010100:Oxygen