Engineering enzymatic cascades on nanoscale scaffolds.
Scaffold proteins are involved in many enzyme cascades in signaling pathways and metabolic processes. The study of scaffolds occurring in biological systems advances at a rapid pace and recently developed engineered synthetic scaffolds enable the precise placement of components of an enzymatic cascade within nanometer distances. Recent experimental results demonstrate significantly increased throughput of enzymatic cascades as a result of the utilization of a scaffold, but our understanding of the mechanisms responsible for this increase is not complete. We discuss the physics of diffusive transport processes relevant for these unique reaction-diffusion systems.
Department of Biomedical Engineering, Columbia University, 351L Engineering Terrace, 1210 Amsterdam Ave., New York, NY 10027, United States.
Ofer
O
Idan
Henry
H
Hess
D057005:Bioengineering; D004058:Diffusion; D004798:Enzymes / Q000737:chemistry*; D053858:Metabolic Networks and Pathways; D036103:Nanotechnology; D011506:Proteins / Q000737:chemistry*; D015398:Signal Transduction
D004798:Enzymes; D011506:Proteins