Proteomic analysis of the tegument and excretory-secretory products of Dicrocoelium dendriticum (Digenea) adult worms.
Dicrocoeliosis, caused by Dicrocoelium dendriticum, is an important hepatic parasitosis in ruminants, whose immunological diagnosis and control remain unsatisfactory. There are very few studies on the antigens of this trematode and molecular knowledge about it is practically nil. Therefore the aim of this study was to identify the major antigenic proteins in the tegument (TG) and excretory-secretory (ES) antigenic extracts of D. dendriticum. The separation conditions of the protein extracts were optimized using 2-D PAGE; the gels were stained with colloidal Coomassie or transferred to carry out immunodetection with anti-Dicrocoelium dendriticum sera. The proteins of interest excised from the gels were identified by mass spectrometry (MALDI). The proteomic maps of the TG and ES extracts of D. dendriticum were defined first, detecting 332 spots in the TG and 284 in the ES, with a similar distribution in both. A quantity of 29 proteins in the excretion-secretion products and 43 in the teguments were identified first in D. dendriticum, 23 of them antigenic, involved in various processes such as: metabolism, detoxification, chaperone, transport or structural molecules. These results could help us to understand the complex parasite-host relationships, improve the diagnosis of dicroceliosis and help to produce possible vaccines to control it.
Departamento de Sanidad Animal, Consejo Superior de Investigaciones Científicas (CSIC), Instituto de Ganadería de Montaña CSIC-ULE, 24346 Grulleros, LEÓN, Spain.
A M
AM
Martínez-Ibeas
C
C
González-Lanza
M Y
MY
Manga-González
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002853:Chromatography, Liquid; D004011:Dicrocoeliasis / Q000469:parasitology / Q000662:veterinary; D004013:Dicrocoelium / Q000737:chemistry* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D005590:Fractional Precipitation; D015801:Helminth Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008099:Liver / Q000469:parasitology; D008297:Male; D020543:Proteome / Q000737:chemistry*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D020543:Proteome