HISTONE DEACETYLASE19 interacts with HSL1 and participates in the repression of seed maturation genes in Arabidopsis seedlings.
The seed maturation genes are specifically and highly expressed during late embryogenesis. In this work, yeast two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays revealed that HISTONE DEACETYLASE19 (HDA19) interacted with the HIGH-LEVEL EXPRESSION OF SUGAR-INDUCIBLE GENE2-LIKE1 (HSL1), and the zinc-finger CW [conserved Cys (C) and Trp (W) residues] domain of HSL1 was responsible for the interaction. Furthermore, we found that mutations in HDA19 resulted in the ectopic expression of seed maturation genes in seedlings, which was associated with increased levels of gene activation marks, such as Histone H3 acetylation (H3ac), Histone H4 acetylation (H4ac), and Histone H3 Lys 4 tri-methylation (H3K4me3), but decreased levels of the gene repression mark Histone H3 Lys 27 tri-methylation (H3K27me3) in the promoter and/or coding regions. In addition, elevated transcription of certain seed maturation genes was also found in the hsl1 mutant seedlings, which was also accompanied by the enrichment of gene activation marks but decreased levels of the gene repression mark. Chromatin immunoprecipitation assays showed that HDA19 could directly bind to the chromatin of the seed maturation genes. These results suggest that HDA19 and HSL1 may act together to repress seed maturation gene expression during germination. Further genetic analyses revealed that the homozygous hsl1 hda19 double mutants are embryonic lethal, suggesting that HDA19 and HSL1 may play a vital role during embryogenesis.
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resource, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Yi
Y
Zhou
Bin
B
Tan
Ming
M
Luo
Yin
Y
Li
Chen
C
Liu
Chen
C
Chen
Chun-Wei
CW
Yu
Songguang
S
Yang
Shuai
S
Dong
Jiuxiao
J
Ruan
Liangbin
L
Yuan
Zhou
Z
Zhang
Linmao
L
Zhao
Chenlong
C
Li
Huhui
H
Chen
Yuhai
Y
Cui
Keqiang
K
Wu
Shangzhi
S
Huang
D000107:Acetylation; D017360:Arabidopsis / Q000166:cytology / Q000196:embryology* / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D008745:Methylation; D009154:Mutation; D009928:Organ Specificity; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000196:embryology / Q000235:genetics / Q000502:physiology; D012639:Seeds / Q000166:cytology / Q000196:embryology / Q000235:genetics / Q000502:physiology; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; C583969:HSL1 protein, Arabidopsis; D006657:Histones; D012097:Repressor Proteins; C507152:HDA19 protein, Arabidopsis; D006655:Histone Deacetylases