[Effect of p53 gene therapy on the local immunity and the efficacy of patients with nasopharyngeal carcinoma].
OBJECTIVE
To investigate the tumor regression and local immune function in nasopharyngeal carcinoma patients treated with p53 gene therapy.


METHODS
The two-step immunohistochemical was done to detect the expression of tumor-infiltrating lymphocytes (TIL) T-cell receptor-CD3, CD4, CD8 and B cell receptor-CD20 in the primary tumor tissue of nasopharyngeal carcinoma. Nasal endoscopy with MRI or CT was used for evaluation of tumor size.


RESULTS
The expression of CD3, CD4, CD8 was significantly increased after p53 gene treatment (P < 0.05). There was no significant change in expression of CD20 after p53 gene treatment (P > 0.05). In conventional treatment group, CD3, CD4, CD8 and CD20 (P > 0.05) did not show any significant difference. In gene therapy group at 3 months after treatment, 20 patients had achieved CR, 10 PR, 1 SD, 1 PD. In conventional treatment group, 11 patients had achieved CR, 12 PR,5 SD,3 PD. The response rate between treatment group and control group (CR+PR) was different (P < 0.05). CD3 and CD4 expression was correlated with tumor regression rate (P < 0.05, P < 0.01), and CD8 expression was correlated with the CR rate (P < 0.05).


CONCLUSIONS
T cells are the most proliferative cell of TII. in NPC patients after p53 gene therapy The local cellular immune status is positively correlated with tumor regression rate.
Department of Otolaryngology-Head and Neck Oncology, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, China.
Yangda
Y
Qin
Jingjin
J
Weng
Guiping
G
Lan
Haiming
H
Wei
Bo
B
Huang
Jinjie
J
Sun
Yongfeng
Y
Si
D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002277:Carcinoma; D005260:Female; D016158:Genes, p53; D015316:Genetic Therapy; D006801:Humans; D018655:Lymphocyte Count; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy*