[The change of calcitonin gene- related peptide in periodontal tissue during orthodontic tooth movement].
OBJECTIVE
To establish a Sprague-Dawley rat model of tooth movement, detect the expression of calcitonin gene-related peptide (calcitonin gene related peptide, CGRP) in Sprague-Dawley rats' periodontal tissues during tooth movement and explore the role of the peripheral mechanisms of pain in orthodontics.


METHODS
60 Sprague-Dawley rats were randomly divided into experimental group (n=54) and control group (n=6). Force was applied on right maxillary first molar in rats and the changes of CGRP expression were detected by real-time PCR 4 h, 8 h, 1 d, 3 d, 5 d, 7 d, 14 d after tooth movement. SPSS 13.0 software package was selected and one-way ANOVA (least significant difference, LSD) was used for statistics analysis.


RESULTS
Experimental tooth movement led to an increase in the expression of CGRP. In addition, following experimental tooth movement, the expression of CGRP in periodontal tissues was significantly up-regulated from 4 h, with a peak on 1d and returned to the control level at 2-week. 1 d after experimental tooth movement, the application of different forces to rats induced a significant increase in CGRP expression.


CONCLUSIONS
The regular change of CGRP expression in periodontal tissues indicates that CGRP may play an important role in peripheral mechanism of orthodontic pain.
Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University. Xi'an 710004, Shanxi Province, China. kouqiang41@163.com
Hu
H
Qiao
Hong
H
Zhou
Yong-Jin
YJ
Zhu
Yu-Nan
YN
Gao
D000818:Animals; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide; D008437:Maxilla; D008963:Molar; D010513:Periodontal Ligament; D010519:Periodontium; D051381:Rats; D017207:Rats, Sprague-Dawley; D014087:Tooth Movement Techniques
D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide