RT-PCR- and MALDI-TOF mass spectrometry-based identification and discrimination of isoforms homologous to pufferfish saxitoxin- and tetrodotoxin-binding protein in the plasma of non-toxic cultured pufferfish (Takifugu rubripes).
Four genes of Takifugu rubripes, tentatively designated Tr1-Tr4, encoding homologs of pufferfish saxitoxin- and tetrodotoxin-binding protein, were identified by BLAST search and 3'-RACE. RT-PCR and MALDI-TOF mass spectrometry allowed the identification and discrimination of Tr isoforms from the non-toxically cultured specimens. The expression of Tr1 and Tr3 mRNAs exclusively in the liver and the presence of their products as 120-kDa plasma proteins were confirmed.
Graduate School of Science and Technology, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Japan.
Ryohei
R
Tatsuno
Kenichi
K
Yamaguchi
Tomohiro
T
Takatani
Osamu
O
Arakawa
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012530:Saxitoxin / Q000378:metabolism; D016415:Sequence Alignment; D015222:Sodium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D023741:Takifugu / Q000097:blood*; D013779:Tetrodotoxin / Q000378:metabolism
D001798:Blood Proteins; D029941:Fish Proteins; D020033:Protein Isoforms; D015222:Sodium Channels; C023790:tetrodotoxin-binding protein; D012530:Saxitoxin; D013779:Tetrodotoxin