Effect of calf age and administration route of initial multivalent modified-live virus vaccine on humoral and cell-mediated immune responses following subsequent administration of a booster vaccination at weaning in beef calves.
OBJECTIVE
To compare immune responses following modified-live virus (MLV) vaccination at weaning after intranasal or SC administration of an MLV vaccine to beef calves at 2 or 70 days of age.


METHODS
184 calves.


METHODS
Calves were allocated to 1 of 5 groups. The IN2 (n = 37) and IN70 (37) groups received an MLV vaccine containing bovine herpesvirus 1 (BHV1), bovine viral diarrhea virus (BVDV) types 1 and 2, bovine respiratory syncytial virus (BRSV), and parainfluenza 3 virus intranasally and a Mannheimia haemolytica and Pasteurella multocida bacterin SC at median ages of 2 and 70 days, respectively. The SC2 (n = 36) and SC70 (37) groups received a 7-way MLV vaccine containing BHV1, BVDV1, BVDV2, BRSV, parainfluenza 3 virus, M haemolytica, and P multocida SC at median ages of 2 and 70 days, respectively; the control group (37) remained unvaccinated until weaning. All calves received the 7-way MLV vaccine SC at median ages of 217 (weaning) and 231 days. Serum neutralizing antibody (SNA) titers against BHV1, BVDV1, and BRSV and intranasal IgA concentrations were determined at median ages of 2, 70, 140, 217, and 262 days. Cell-mediated immunity (CMI) against BHV1, BRSV, BVDV1, and P multocida was determined for 16 calves/group.


RESULTS
At median ages of 140 and 217 days, BVDV1 SNA titers were significantly higher for the SC70 group than those for the other groups. Intranasal IgA concentrations and CMI increased over time for all groups. Vaccination at weaning increased SNA titers and CMI in all groups.


CONCLUSIONS
SC administration of an MLV vaccine to 70-day-old calves significantly increased BVDV1 antibody titers before weaning.
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. awoolums@uga.edu
Amelia R
AR
Woolums
Roy D
RD
Berghaus
Londa J
LJ
Berghaus
Roger W
RW
Ellis
Mel E
ME
Pence
Jeremiah T
JT
Saliki
Katherine A E
KA
Hurley
Kimberly L
KL
Galland
William W
WW
Burdett
Scott T
ST
Nordstrom
David J
DJ
Hurley
D000281:Administration, Intranasal / Q000662:veterinary; D000367:Age Factors; D000818:Animals; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007114:Immunization / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D011897:Random Allocation; D012141:Respiratory Tract Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014886:Weaning
D014613:Vaccines, Attenuated; D017778:Vaccines, Combined; D014765:Viral Vaccines