CrcZ and CrcX regulate carbon source utilization in Pseudomonas syringae pathovar tomato strain DC3000.
Small non-coding RNAs (ncRNAs) are important components of many regulatory pathways in bacteria and play key roles in regulating factors important for virulence. Carbon catabolite repression control is modulated by small RNAs (crcZ or crcZ and crcY) in Pseudomonas aeruginosa and Pseudomonas putida. In this study, we demonstrate that expression of crcZ and crcX (formerly designated psr1 and psr2, respectively) is dependent upon RpoN together with the two-component system CbrAB, and is influenced by the carbon source present in the medium in the model plant pathogen Pseudomonas syringae pv tomato DC3000. The distribution of the members of the Crc ncRNA family was also determined by screening available genomic sequences of the Pseudomonads. Interestingly, variable numbers of the Crc family members exist in Pseudomonas genomes. The ncRNAs are comprised of three main subfamilies, named CrcZ, CrcX and CrcY. Most importantly the CrcX subfamily appears to be unique to all P. syringae strains sequenced to date.
Plant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY, USA. melanie.filiatrault@ars.usda.gov
Melanie J
MJ
Filiatrault
Paul V
PV
Stodghill
Janet
J
Wilson
Bronwyn G
BG
Butcher
Hanrong
H
Chen
Christopher R
CR
Myers
Samuel W
SW
Cartinhour
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002244:Carbon / Q000378:metabolism*; D057465:Catabolite Repression; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; C432141:CbrA protein, Pseudomonas aeruginosa; C432142:CbrB protein, Pseudomonas aeruginosa; D012329:RNA, Bacterial; D058727:RNA, Small Untranslated; D014157:Transcription Factors; D002244:Carbon
CbrA; CbrB; Crc; Pseudomonas syringae; RpoN; crcX; crcY; crcZ; ncRNA; psr