A new betasatellite associated with cotton leaf curl Burewala virus infecting tomato in India: influence on symptoms and viral accumulation.
A begomovirus and its associated alpha- and betasatellite were detected in tomato plants affected with leaf curl disease. Based on a nucleotide sequence identity of 99 %, this begomovirus was designated an isolate of cotton leaf curl Burewala virus (CLCuBuV). The alphasatellite exhibited 93 % sequence identity to cotton leaf curl Burewala alphasatellite (CLCuBuA) and is hence referred to here as a variant of CLCuBuA. The detected betasatellite was recombinant in nature and showed 70 % sequence identity to the known betasatellites. Inoculation of healthy tomato with CLCuBuV plus betasatellite, either in the presence or the absence of alphasatellite, led to typical leaf curling, while inoculation with CLCuBuV in the absence of betasatellite resulted in mild symptoms. This confirmed the role of the betasatellite in expression of disease symptoms. We propose to name the newly detected betasatellite tomato leaf curl Hajipur betasatellite (ToLCHJB).
National Agri-Food Biotechnology Institute, Mohali, 160071 Punjab, India. jitsingh27281@gmail.com
Jitendra
J
Kumar
Samatha
S
Gunapati
Sudhir P
SP
Singh
Abhinav
A
Kumar
Adarsh
A
Lalit
Naresh C
NC
Sharma
Rekha
R
Puranik
Rakesh
R
Tuli
D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D003368:Gossypium / Q000821:virology; D007194:India; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology*
D004276:DNA, Satellite