Osteopromotive activity of a novel pyrazole carboxamide derivative.
OBJECTIVE
To investigate the effect of 1-(4-(tert-butyl)benzyl)-N-(4-methoxyphenyl)-3-phenyl-1H-pyrazole-5-carboxamide (Pyr-C) on the proliferation and osteogenic differentiation of MC3T3-E1 cells.


METHODS
MTT and BrdU incorporation assay were used to determine cell survival and proliferation. The gene expression levels of osteogenic markers were determined using real-time PCR and ALP activity was detected. Western-blot analysis was used to determine the protein expression of BSP and OPN. The long-term effect of Pyr-C on mineralization deposition was measured by Alizarin Red Staining.


RESULTS
Pyr-C inhibited cell proliferation and increased ALP activity. Gene expression of ALP, BSP, OCN, Runx2, and Osterix was up-regulated in Pyr-C-induced group. Pyr-C increased the protein expression of BSP at day 7, 14 and 21, and OPN at day 14, 21 and 28. Meanwhile, Pyr-C enhanced the mineral deposition.


CONCLUSIONS
Pyr-C inhibits proliferation and stimulates osteogenic differentiation of MC3T3-E1 cells.
Department of Periodontology, School of Stomatology, Shandong University, Jinan, 250012, P.R. China.
Qian-Qian
QQ
Han
Mei-Jiao
MJ
Yu
Shao-Hua
SH
Ge
Hong-Mei
HM
Guo
Jin
J
Zhang
Bao-Xiang
BX
Zhao
Pi-Shan
PS
Yang
D016475:3T3 Cells; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011720:Pyrazoles / Q000737:chemistry / Q000494:pharmacology*
D011720:Pyrazoles