Heavy metal influence on BDE-47 uptake in the human KERTr keratinocyte cell line.
Significant correlations between concentrations of PBDEs and heavy metals were observed in the human body. However, there is a lack of evidence on the linkage between the uptake of heavy metals and PBDEs. This study is the first report on the BDE-47 uptake profile in a human cell line. Hg and As exposures to KERTr (human skin derived keratinocyte) did not significantly (p > 0.05) affect the uptake of BDE47, whereas Pb and Cd significantly (p < 0.05) affected the uptake of BDE-47 in KERTr. The change in Km was minor after exposure to all heavy metals. The maximum transport rate (Vmax) after exposure to Pb (Vmax: 5.23 ± 0.49) and As (Vmax: 4.95 ± 0.60) was significantly increased when compared with the background of the KERTr cell line (Vmax: 4.07 ± 0.35). Real-time RT-PCR indicated that OATP-B, OATP-D, and OATP-E were expressed in the KERTr cell line. The upregulation or downregulation of OATP B and D genes were minor after exposure to heavy metals, but the OATP E gene was upregulated by three to fourfold in KERTr cell line after exposure to Pb an Cd, which may explain the significant increase of uptake of BDE-47 in KERTr after exposures to Pb and Cd. This study indicated that the uptake effects should be considered when performing risk assessment of human exposure to PBDEs and heavy metal.
School of Chemistry and Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Croucher Institute for Environmental Sciences and Department of Biology, Kowloon Tong, Hong Kong, People's Republic of China.
Yuan
Y
Kang
Yi
Y
Yin
Qiu Yun
QY
Zhang
Lai Sheng
LS
Li
Li Xuan
LX
Zeng
Ji Wen
JW
Luo
Ming Hung
MH
Wong
D001151:Arsenic / Q000493:pharmacokinetics; D002104:Cadmium / Q000493:pharmacokinetics; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000493:pharmacokinetics*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D007854:Lead / Q000493:pharmacokinetics; D008628:Mercury / Q000493:pharmacokinetics; D019216:Metals, Heavy / Q000493:pharmacokinetics*; D027361:Organic Anion Transporters / Q000235:genetics / Q000378:metabolism
D055768:Halogenated Diphenyl Ethers; D019216:Metals, Heavy; D027361:Organic Anion Transporters; D002104:Cadmium; C511295:2,2',4,4'-tetrabromodiphenyl ether; D007854:Lead; D008628:Mercury; D001151:Arsenic
KERTr; PBDEs; heavy metals; uptake