A modular, low-cost robot for zebrafish handling.
The zebrafish (danio rerio) is one of the most important model organisms in modern drug discovery and disease modeling. Handling and analyzing large numbers of zebrafish larvae require an immense manpower and involve time-consuming manual processes. A novel modular, robotic platform for high-throughput screening is being developed at BioRobotLab (KIT). In this article the fish sorter, which is a robotic device for the automation of a manual process in bio analysis, is presented. The fish sorter detects randomly spread zebrafish eggs and larvae up to an age of 120 hours post fertilization (hpf) in Petri dishes and transfers them to standard 96- or 384- well plates. The robot is controlled by an advanced algorithm with sensor-based process control. Fast and precise hardware components lead to a high working speed and success rate >= 95%.
Institute of Applied Computer Science, Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany. alexander.pfriem@kit.edu
Alexander
A
Pfriem
Christian
C
Pylatiuk
Rüdiger
R
Alshut
Bertram
B
Ziegener
Stefan
S
Schulz
Georg
G
Bretthauer
D000465:Algorithms; D000818:Animals; D012371:Robotics; D015027:Zebrafish