Fabrication of FITC-doped silica nanoparticles and study of their cellular uptake in the presence of lectins.
Fluorescent silica nanoparticles are reported to be highly stable and biocompatible materials with high water solubility, which make them ideal candidates for biological applications. These nanoparticles can also be modified with biocompatible and targeting moieties and can be used for a variety of in vitro and in vivo applications, such as targeting, particle tracking, cargo carrier, and as contrast agents. In this study, fluorescent dye-doped silica nanoparticles were prepared by a modified Stöber method. The nanoparticles produced were surface functionalized with amine moieties for their conjugation with glucose-derived and galactose-based residues. The amine, glucose-derived, and galactose-based functionalized fluorescent silica nanoparticles were analyzed for their physiochemical properties such as sizes, polydispersities, organic layer content, and surface chemistries. The nanoparticles produced were then studied for their interactions with carbohydrate-specific lectins. These lectin bioconjugates have helped in understanding their interactions with cell-surface receptors. As expected, galactose-functionalized nanoparticles were found to specifically interact with RCA120 , as compared to other nanoparticles. These specific interactions of galactose-lectin conjugates were further studied on the hepatocytes cell surface in vitro. The aggregation of galactose-lectins conjugates on the plasma membrane was possibly due to the specific interactions of carbohydrates with cell-surface glycoproteins, hence preventing the uptake of these nanoparticles. The study has provided an interesting approach to mark the cell-surface glycoproteins with fluorescent probes using a combination of lectin-carbohydrate conjugates.
Biomaterials Unit, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Yohei
Y
Kotsuchibashi
Yiyao
Y
Zhang
Marya
M
Ahmed
Mitsuhiro
M
Ebara
Takao
T
Aoyagi
Ravin
R
Narain
D002241:Carbohydrates / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004563:Electrochemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D008027:Light; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D012276:Ricin / Q000494:pharmacology*; D012542:Scattering, Radiation; D012822:Silicon Dioxide; D017550:Spectroscopy, Fourier Transform Infrared; D013778:Tetrazolium Salts; D013818:Thermogravimetry; D013844:Thiazoles
D002241:Carbohydrates; D005456:Fluorescent Dyes; D013778:Tetrazolium Salts; D013844:Thiazoles; D012822:Silicon Dioxide; D012276:Ricin; C022616:thiazolyl blue; D016650:Fluorescein-5-isothiocyanate; D005690:Galactose