Mineral density, morphology and bond strength of natural versus artificial caries-affected dentin.
This study aimed to investigate an artificial caries-affected dentin (ACAD) model for in vitro bonding studies in comparison to natural caries-affected dentin (NCAD) of human teeth. ACAD was created over 7 days in a demineralizing solution. Mineral density (MD) at different depth levels (0-150 µm) was compared between NCAD and ACAD by transverse microradiography. Micro-tensile bond strengths (µTBS) of two two-step self-etch adhesives to sound dentin, NCAD and ACAD were evaluated. Caries-affected dentin type was not a significant factor when comparing MD at different lesion levels (p>0.05). Under SEM, the dentinal tubules appeared occluded with crystal logs 1-2 µm in thickness in the NCAD; whereas they remained open in the ACAD. The µTBS to caries-affected dentin was lower than sound dentin, but was not affected by the type of caries (p>0.05). In spite of their different morphologies, the ACAD model showed similar MD and µTBS compared to NCAD.
Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Gerardo José
GJ
Joves
Go
G
Inoue
Syozi
S
Nakashima
Alireza
A
Sadr
Toru
T
Nikaido
Junji
J
Tagami
D000704:Analysis of Variance; D015519:Bone Density; D003731:Dental Caries / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D003804:Dentin / Q000737:chemistry* / Q000473:pathology / Q000503:physiopathology; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008852:Microradiography; D008855:Microscopy, Electron, Scanning; D003765:Models, Dental; D013718:Tensile Strength
D017220:Dentin-Bonding Agents