Improving molecular discrimination of Nosema apis and Nosema ceranae.
Nosema apis and Nosema ceranae are the causative agents of nosemosis, a contagious honeybee disease that weakens bee colonies. The species are discriminated through several PCR-based methods including a multiplex PCR recommended by the World Organization for Animal Health (OIE). In this study, the OIE protocol was compared to two other PCR protocols using different PCR kits with the same primer pairs as described in OIE. The results showed that the three PCR protocols have similar sensitivity but only the kit dedicated to multiplex PCR could detect small quantities of one Nosema species when greater quantities of the other were also present. However, singleplex PCR methods are currently the most sensitive methods for discerning each species. These results have important implications for epidemiology and the understanding of the disease.
Anses, Sophia Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France.
Jérôme
J
Carletto
Philippe
P
Blanchard
Aurélie
A
Gauthier
Frank
F
Schurr
Marie-Pierre
MP
Chauzat
Magali
M
Ribière
D000818:Animals; D001516:Bees / Q000382:microbiology; D002965:Classification / Q000379:methods; D016818:Nosema / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D013045:Species Specificity