Isolation of rare cancer cells from blood cells using dielectrophoresis.
In this study, we investigate the application of contactless dielectrophoresis (cDEP) for isolating cancer cells from blood cells. Devices with throughput of 0.2 mL/hr (equivalent to sorting 3×10(6) cells per minute) were used to trap breast cancer cells while allowing blood cells through. We have shown that this technique is able to isolate cancer cells in concentration as low as 1 cancer cell per 10(6) hematologic cells (equivalent to 1000 cancer cells in 1 mL of blood). We achieved 96% trapping of the cancer cells at 600 kHz and 300 V(RMS).
Engineering Science and Mechanics Department, Virginia Tech and with the Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Blacksburg, VA 24061, USA.
Alireza
A
Salmanzadeh
Michael B
MB
Sano
Hadi
H
Shafiee
Mark A
MA
Stremler
Rafael V
RV
Davalos
D001773:Blood Cells / Q000473:pathology; D001943:Breast Neoplasms / Q000097:blood / Q000473:pathology; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005260:Female; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009360:Neoplastic Cells, Circulating / Q000473:pathology*