Preparation of a molecularly imprinted soft contact lens as a new ocular drug delivery system for dorzolamide.
In the present work a series of imprinted (MIPs) and non-imprinted (NIPs) hydrogels were prepared using 2-hydroxyethyl methacrylate (HEMA) as a backbone monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker monomer, methacrylic acid (MAA) as a functional monomer and dorzolamide (DZD) as the template molecule. Different concentrations of MAA (0, 100, 200, 400 mM) were used for preparation of NIPs. Two DZD: MAA molar ratios (1:8 and 1:4) and 400 mM MAA were also applied in imprinting process. The hydrogels (0.4 mm thickness) were synthesized by thermal polymerization at 50°C in 24h in a polypropylene mould. Then, the swelling and binding properties of hydrogels were evaluated in water. Their loading and releasing properties were also studied in NaCl 0.9% and artificial lachrymal fluid. The results showed that using MAA as co-monomer and applying molecular imprinting technique increased loading capacity of hydrogels. The optimized imprinted hydrogel (MIP1:4), prepared with 400 mM MAA and DZD: MAA molar ratio of 1:4, had the highest affinity for DZD and the greatest ability to control the release process in aqueous media. Our data indicated that the use of suitable co-monomer and applying a molecular imprinting technique had important influence on loading and releasing properties of hydrogels.
Nanotechnology Research Centre, School of pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Bizhan
B
Malaekeh-Nikouei
Seyedeh Atefeh
SA
Vahabzadeh
Seyed Ahmad
SA
Mohajeri
D060433:Administration, Ophthalmic; D000327:Adsorption; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry*; D003263:Contact Lenses, Hydrophilic; D016503:Drug Delivery Systems; D020100:Hydrogels; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D013449:Sulfonamides / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
D002257:Carbonic Anhydrase Inhibitors; D020100:Hydrogels; D008689:Methacrylates; D013449:Sulfonamides; D013876:Thiophenes; C008384:methacrylic acid; C005044:hydroxyethyl methacrylate; C004919:ethylene dimethacrylate; C062765:dorzolamide