Simultaneous measurement of amyloid fibril formation by dynamic light scattering and fluorescence reveals complex aggregation kinetics.
An apparatus that combines dynamic light scattering and Thioflavin T fluorescence detection is used to simultaneously probe fibril formation in polyglutamine peptides, the aggregating subunit associated with Huntington's disease, in vitro. Huntington's disease is a neurodegenerative disorder in a class of human pathologies that includes Alzheimer's and Parkinson's disease. These pathologies are all related by the propensity of their associated protein or polypeptide to form insoluble, β-sheet rich, amyloid fibrils. Despite the wide range of amino acid sequence in the aggregation prone polypeptides associated with these diseases, the resulting amyloids display strikingly similar physical structure, an observation which suggests a physical basis for amyloid fibril formation. Thioflavin T fluorescence reports β-sheet fibril content while dynamic light scattering measures particle size distributions. The combined techniques allow elucidation of complex aggregation kinetics and are used to reveal multiple stages of amyloid fibril formation.
Department of Applied Physics, Stanford University, Stanford, California, USA.
Aaron M
AM
Streets
Yannick
Y
Sourigues
Ron R
RR
Kopito
Ronald
R
Melki
Stephen R
SR
Quake
D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D000595:Amino Acid Sequence; D000682:Amyloid / Q000096:biosynthesis / Q000648:ultrastructure*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism; D052160:Benzothiazoles; D005453:Fluorescence; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism / Q000473:pathology; D007700:Kinetics; D008027:Light; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology; D010316:Particle Size; D017433:Protein Structure, Secondary; D013844:Thiazoles / Q000737:chemistry
D000682:Amyloid; D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D013844:Thiazoles; C009462:thioflavin T