Invasive properties, adhesion patterns and phylogroup profiles among Escherichia coli strains isolated from children with inflammatory bowel disease.
BACKGROUND
A great deal of evidence indicates a link between Escherichia coli (E. coli) and Crohn's disease in adult patients, but there is lack of information on the association of these bacilli with inflammatory bowel disease (IBD) among children.


OBJECTIVE
The study was carried out to determine the distribution of phylogenetic group, the adherence patterns and invasive properties of E. coli isolated from children with IBD and non-IBD chronic bowel diseases.


METHODS
A total of 22 E. coli isolated from biopsy specimens from children with IBD and 21 E. coli strains obtained from children with indeterminate colitis and intestinal polyps were examined for adherence and internalization to the Int407 cell line. Genes involved in epithelial cell invasion and genes specific to E. coli phylogroups were determined by polymerase chain reaction (PCR).


RESULTS
The undefined adherence pattern predominated among the isolated E. coli, although most of them demonstrated the afaD and aggB genes encoding invasions of diffusely adhering and enteroaggregative E. coli. Regardless of the clinical entity, most E. coli were internalized by Int407 epithelial cells and belonged to the B2 and D phylogroups.


CONCLUSIONS
The wide distribution of adhesive E. coli capable of entering Int407 cells but also having genes encoding adhesins and invasins characteristic to pathogenic E. coli strains seems to indicate that these E. coli may represent a large group of pathogenic E. coli strains contributing to chronic intestinal disorders.
Department of Microbiology, Wroclaw Medical University, Wrocław, Poland. bmsobie@gmail.com
Beata A
BA
Sobieszczańska
Anna B
AB
Duda-Madej
Michał B
MB
Turniak
Roman
R
Franiczek
Urszula
U
Kasprzykowska
Anna K
AK
Duda
Marta
M
Rzeszutko
Barbara
B
Iwańczak
D018830:Adhesins, Escherichia coli / Q000235:genetics; D000293:Adolescent; D000367:Age Factors; D001422:Bacterial Adhesion / Q000235:genetics; D001706:Biopsy; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D018830:Adhesins, Escherichia coli; C108510:AfaD protein, E coli; D029968:Escherichia coli Proteins; C088705:aggB protein, E coli