Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts.
To understand the regulation mechanism of NaCl on glucosinolate metabolism in broccoli sprouts, the germination rate, fresh weight, contents of glucosinolates and sulforaphane, as well as myrosinase activity of broccoli sprouts germinated under 0, 20, 40, 60, 80, and 100 mmol/L of NaCl were investigated in our experiment. The results showed that glucoerucin, glucobrassicin, and 4-hydroxy glucobrassicin in 7-d-old broccoli sprouts were significantly enhanced and the activity of myrosinase was inhibited by 100 mmol/L of NaCl. However, the total glucosinolate content in 7-d-old broccoli sprouts was markedly decreased although the fresh weight was significantly increased after treatment with NaCl at relatively low concentrations (20, 40, and 60 mmol/L). NaCl treatment at the concentration of 60 mmol/L for 5 d maintained higher biomass and comparatively higher content of glucosinolates in sprouts of broccoli with decreased myrosinase activity. A relatively high level of NaCl treatment (100 mmol/L) significantly increased the content of sulforaphane in 7-d-old broccoli sprouts compared with the control. These results indicate that broccoli sprouts grown under a suitable concentration of NaCl could be desirable for human nutrition.
Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
Rong-fang
RF
Guo
Gao-feng
GF
Yuan
Qiao-mei
QM
Wang
D001937:Brassica / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005961:Glucosinolates / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D017879:Isothiocyanates; D035261:Plant Components, Aerial / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000008:administration & dosage*; D013454:Sulfoxides; D013861:Thiocyanates / Q000378:metabolism*
D005961:Glucosinolates; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; D012965:Sodium Chloride; D006026:Glycoside Hydrolases; C022739:thioglucosidase; C016766:sulforaphane