Associations between serum bone-specific alkaline phosphatase activity, biochemical parameters, and functional polymorphisms of the tissue-nonspecific alkaline phosphatase gene in a Japanese population.
BACKGROUND
We had demonstrated that single nucleotide polymorphism (787T>C) in the tissue-nonspecific ALP (TNSALP) gene was associated with the bone mineral density (BMD). BMD was the lowest among TNSALP 787T homozygotes (TT-type) and highest among TNSALP 787T>C homozygotes (CC-type) in postmenopausal women. In the present study, we investigated the effects of the TNSALP genotype on associations among serum bonespecific alkaline phosphatase (BAP), serum calcium, and phosphorus in healthy young Japanese subjects.


METHODS
Young healthy adult subjects (n=193) were genotyped for the polymorphism, and we measured the levels of serum BAP, serum calcium, and phosphorus. Dietary nutrient intakes were calculated based on 3-day food records before the day of blood examinations.


RESULTS
Grouped by the TNSALP genotype, a significant negative correlation between serum BAP and phosphorus was observed in 787T>C homozygotes (CC-type), but not in heterozygotes (TCtype), nor in 787T homozygotes (TT-type).


CONCLUSIONS
In the present study, we revealed that the single nucleotide polymorphism 787T>C in the TNSALP gene had effects on the correlation between serum BAP and phosphorus in young adult subjects. These results suggest that variation in TNSALP may be an important determinant of phosphate metabolism. Our data may be useful for planning strategies to prevent osteoporosis.
Division of Nutrition, Department of Food and Nutrition, Faculty of Human Sciences and Design, Japan Women's University, Tokyo, Japan.
Natsuko
N
Sogabe
Rieko
R
Tanabe
Mayu
M
Haraikawa
Yutaka
Y
Maruoka
Hideo
H
Orimo
Takayuki
T
Hosoi
Masae
M
Goseki-Sone
D000469:Alkaline Phosphatase / Q000097:blood* / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D001842:Bone and Bones / Q000737:chemistry*; D002136:Calcium, Dietary / Q000097:blood; D002149:Energy Intake; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007564:Japan; D008297:Male; D010024:Osteoporosis / Q000235:genetics / Q000517:prevention & control; D016226:Phosphorus, Dietary / Q000097:blood; D020641:Polymorphism, Single Nucleotide; D014807:Vitamin D / Q000097:blood; D055815:Young Adult
D002136:Calcium, Dietary; D016226:Phosphorus, Dietary; D014807:Vitamin D; C548267:ALPL protein, human; D000469:Alkaline Phosphatase