Differential pulse polarographic investigation of the antifungal drugs itraconazole, ketoconazole, fluconazole and voriconazole using a dropping mercury electrode.
The electrochemical reactions of the antifungal drugs itraconazole, ketoconazole, fluconazole and voriconazole have been investigated by differential pulse polarography (DPP) using a dropping mercury electrode (DME). All investigations were carried out in Britton-Robinson buffer solutions and methanol with varying pH values. Ketoconazole and itraconazole both showed a reduction peak with a potential between -1.5V and -1.6 V. Stable and reproducible conditions for the determination of itraconazole (c = 1 x 10(-7) M) were found within the pH range of 6.0 to 8.0 and for the determination of ketoconazole (c = 5 x 10(-8) M) within pH 6.0 to 7.0. Voriconazole showed a reduction peak with a peak potential of -1.7 V (c = 1 x 10(-5) M) within the pH range of 8.0 to 10.0. In the case of fluconazole no electrochemical activity was found.
Pharmacy Department, University Hospital Carl Gustav Carus TU Dresden, Germany. holger.knoth@uniklinikum-dresden.de
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D000935:Antifungal Agents / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D015725:Fluconazole / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D017964:Itraconazole / Q000032:analysis; D007654:Ketoconazole / Q000032:analysis; D008628:Mercury; D011048:Polarography / Q000379:methods; D011743:Pyrimidines / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D014230:Triazoles / Q000032:analysis; D065819:Voriconazole
D000935:Antifungal Agents; D007202:Indicators and Reagents; D011743:Pyrimidines; D014230:Triazoles; D017964:Itraconazole; D015725:Fluconazole; D008628:Mercury; D065819:Voriconazole; D007654:Ketoconazole