Profiling and characterization of volatile components from non-fumigated and sulfur-fumigated Flos Lonicerae Japonicae using comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry coupled with chemical group separation.
Flos Lonicerae Japonicae (FLJ) is a popular herb used for many centuries in Traditional Chinese Medicine as a treatment of fever and inflammation. Non-fumigated processing of FLJ has been the traditional approach used in post-harvest preparation of the commodity for commercial use. However, in recent years, natural drying processing of FLJ has been replaced by sulfur-fumigation for efficiency and pest control. Sulfur-fumigation can induce changes in the volatile compounds of the herb, altering its medicinal properties. A comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOF/MS) method was established for the resolution and determination of volatile components in non-fumigated and sulfur-fumigated FLJ. In this paper, analysis of the volatile oils in non-fumigated and sulfur-fumigated (including lab-prepared sulfur-fumigated and industrial sulfur-fumigated) FLJ was performed using GC×GC-TOF/MS. Seventy-three representative volatile components were identified, including furans, alkalies, acids, aldehydes, ketones, alcohols, terpenes, esters, and others, as the main components of FLJ volatile oils. The proposed method was successfully applied for rapid and accurate quality evaluation of FLJ and its related medicinal materials and preparations.
Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. haocai_98@126.com
Hao
H
Cai
Gang
G
Cao
Li
L
Li
Xiao
X
Liu
Xiao-Qing
XQ
Ma
Si-Cong
SC
Tu
Ya-Jing
YJ
Lou
Kun-Ming
KM
Qin
Song-Lin
SL
Li
Bao-Chang
BC
Cai
D005651:Fumigation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D031239:Lonicera / Q000737:chemistry* / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D045792:Norisoprenoids / Q000032:analysis / Q000737:chemistry; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis / Q000737:chemistry; D013455:Sulfur / Q000494:pharmacology*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry*
D045792:Norisoprenoids; D009822:Oils, Volatile; D012717:Sesquiterpenes; D055549:Volatile Organic Compounds; D013455:Sulfur; C011879:alpha-ionone