Effects of faba bean and faba bean hulls on expression of selected genes in the small intestine of piglets.
In a small intestinal segment perfusion (SISP) study in pigs, effects were studied of intestinal perfusion of ground faba beans (Vicia faba), faba bean hulls, or saline on intestinal net fluid absorption in intestinal segments either challenged or not with an enterotoxigenic Escherichia coli (ETEC). After an 8-h perfusion, piglets were euthanized and small intestinal mucosa samples were collected for analysis of expression level of a selected set of genes (APOC3, TIMP1, AQP8, MMP1, MUC13, and PAP) using real-time quantitative PCR (qPCR). Perfusion with ground faba beans and faba bean hulls and challenge with ETEC affected (P < 0.05) expression of several of genes in the intestinal mucosa. Expression of APOC3, TIMP1, AQP8, MMP1, and PAP was correlated with net fluid absorption in the small intestine of pigs.
Wageningen UR Livestock Research, 8200 AB Lelystad, The Netherlands. Alfons.Jansman@wur.nl
A J M
AJ
Jansman
J
J
van Baal
J
J
van der Meulen
M A
MA
Smits
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary; D005786:Gene Expression Regulation / Q000502:physiology*; D007413:Intestinal Mucosa; D007421:Intestine, Small / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine / Q000378:metabolism*; D031307:Vicia faba / Q000737:chemistry*