[Rapid propagation of Bletilla striata by synthetic seeds technology].
OBJECTIVE
To establish a new manufacturing method for Bletilla striata synthetic seeds, and provided a new way for rapid propagation of B. striata, the correlated influential factors were studied.


METHODS
The synthetic seeds were manufactured by taking seeds of B. striata as materials which were beforehand germinated in 1/2 MS medium for 10 days, and the influential factors such as artificial endosperm components, episperm substances, storage conditions and germination groundmass impact on the germination rate and seedling rate of the synthetic seeds were evaluated.


RESULTS
Compound 4.0% sodium alginate + 0.2 mol x L(-1) CaCl2 + 0.4 mg x L(-1) penicillin + 0.3% carbendazim powder + 0.2% sodium benzoate served as the best episperm substances while MS + 1.0 mg x L(-1) NAA + 2.0 mg x L(-1) KT as the best endosperm components, in which, high germination rate and seedling rate were obtained. The synthetic seeds storing in the 4 degrees C for a long time was able to have still high vitality.


CONCLUSIONS
The B. striata synthetic seeds manufacturing system was established successfully, while efforts should be taken to improve the sowing technique of the synthetic seeds in non-sterile conditions.
(Zhejiang Chinese Medical University, Hangzhou 310053, China. lwp19880612@163.com
Wei-Ping
WP
Li
Sha-Sha
SS
Tian
Guang-Yao
GY
Lu
Di
D
Lv
Yang-Ni
YN
Wang
Fu-Sheng
FS
Jiang
Zhi-Shan
ZS
Ding
D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D018525:Germination; D029595:Orchidaceae / Q000254:growth & development* / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media