Simultaneous determination of 13 fluoroquinolone and 22 sulfonamide residues in milk by a dual-colorimetric enzyme-linked immunosorbent assay.
Enzyme-linked immunosorbent assays (ELISAs) usually focus on the detection of a single analyte or a single group of analytes, e.g., fluoroquinolones or sulfonamides. However, it is often necessary to simultaneously monitor two classes of antimicrobial residues in different food matrixes. In this paper, we describe a dual-colorimetric ELISA for the simultaneous detection of 13 fluoroquinolone and 22 sulfonamide residues. The limit of detection for fluoroquinolones and sulfonamides was 2.4 and 5.8 ng/mL, respectively. The developed immunoassay is suitable for high-throughput screening of these low-molecular weight contaminants. This is the first report where two different enzymes (alkaline phosphatase and horseradish peroxidase) were used in one immunoassay and together in a single well for simultaneous detection of multiple low-molecular weight chemical residues.
Wenxiao
W
Jiang
Zhanhui
Z
Wang
Ross C
RC
Beier
Haiyang
H
Jiang
Yongning
Y
Wu
Jianzhong
J
Shen
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis* / Q000378:metabolism; D002417:Cattle; D003124:Colorimetry; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D024841:Fluoroquinolones / Q000032:analysis / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis*; D006735:Horseradish Peroxidase / Q000378:metabolism; D008892:Milk / Q000737:chemistry*; D013449:Sulfonamides / Q000032:analysis / Q000378:metabolism
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D013449:Sulfonamides; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase