Transsutural distraction and tissue regeneration of the midfacial skeleton: experimental studies in growing dogs.
Objective : The purpose of this study was to evaluate the effect of different mechanical forces on the expansion of the palatine suture using transsutural distraction osteogenesis. Methods : A total of 48 dogs were used in this study. The experimental groups were treated with a custom-designed internal distractor. Bone regeneration was determined with x-rays and histology. The computed values underwent statistical analyses using analysis of variance. Results : The maxillary complex was most noticeably advanced with an applied mechanical force of 600 g (20.15 ± 1.36 mm), compared with forces of 400 g (19.88 ± 1.41 mm) and 800 g (2.24 ± 0.93 mm). Immunohistochemical staining showed that the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 fluctuated with different mechanical forces. These changes were statistically significant when 600 g of force was applied within 30 days of distraction (P < .05). Conclusions : Transsutural distraction osteogenesis in the growing dog should be safe and well tolerated in inducing bony lengthening of the maxilla, and the optimal force is 600 × g. Bone morphogenetic protein-2 and bone morphogenetic protein-4 may play an important roles in the signaling pathways that link mechanical forces and biological responses.
Yao
Y
Yusheng
Wang
W
Chengyue
Wang
W
Zhiying
Wang
W
Guijun
D000818:Animals; D001696:Biomechanical Phenomena; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D016723:Bone Remodeling / Q000502:physiology; D003393:Cranial Sutures / Q000601:surgery*; D004285:Dogs; D004867:Equipment Design; D007150:Immunohistochemistry; D023421:Models, Animal; D019857:Osteogenesis, Distraction / Q000295:instrumentation / Q000379:methods*
D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4
BMPs; bone regeneration; midfacial hypoplasia; transsutural distraction osteogenesis