Modulation of Fourier transform infrared spectra and total sialic acid levels by selenium during 1,2 dimethylhydrazine-induced colon carcinogenesis in rats.
The present study investigated the modulatory potential of selenium supplementation, if any, on Fourier transform infrared (FTIR) spectra in brush border membranes (BBM) of colons and on serum total sialic acid as well as lipid bound sialic acid during 1,2 dimethyl hydrazine (DMH)-induced colorectal carcinogenesis in rats. The FTIR spectra of BBM from the colons of DMH-treated rats revealed a significant increase in the lipid contents but showed a significant decline in the protein contents. Further, decrease in the collagen as well as creatine contents was also noticed in the colons of DMH-treated rats. Supplementation with selenium appreciably restored protein as well as collagen contents and resulted in decreased lipids levels in the colons of DMH-treated rats. Interestingly, a significant increase in the levels of total sialic acid in serum of DMH-treated rats was observed which, however, got moderated significantly upon selenium supplementation. Moreover, no significant changes were observed in the levels of lipid bound sialic acid in all the treated groups as compared to controls. In conclusion, the present study suggested that supplementation of selenium act as a chemopreventive agent and delays considerably the process of colon carcinogenesis.
Department of Biophysics, Panjab University, Chandigarh, India.
Fereshteh Ezzati
FE
Ghadi
Anshoo
A
Malhotra
Abdollah Ramzani
AR
Ghara
D K
DK
Dhawan
D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control*; D008297:Male; D008871:Microvilli / Q000187:drug effects; D019158:N-Acetylneuraminic Acid / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
D016588:Anticarcinogenic Agents; D003094:Collagen; D019158:N-Acetylneuraminic Acid; D012643:Selenium; D019813:1,2-Dimethylhydrazine