Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections.
In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.
The Danish Technological Institute, Life Science Division, Aarhus, Denmark.
Tine Y
TY
Wolff
Steffen
S
Eickhardt
Maria K
MK
Björnsdottir
Claus
C
Moser
Thomas
T
Bjarnsholt
Niels
N
Høiby
Trine R
TR
Thomsen
D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D060085:Coinfection / Q000175:diagnosis* / Q000382:microbiology*; D019295:Computational Biology / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software