Trafficking of the follitropin receptor.
The follitropin or follicle-stimulating hormone receptor (FSHR) belongs to a highly conserved subfamily of the G protein-coupled receptor (GPCR) superfamily and is mainly expressed in specific cells in the gonads. As any other GPCR, the newly synthesized FSHR has to be correctly folded and processed in order to traffic to the cell surface plasma membrane and interact with its cognate ligand. In this chapter, we describe in detail the conditions and procedures used to study outward trafficking of the FSHR from the endoplasmic reticulum to the plasma membrane. We also describe some methods to analyze phosphorylation, β-arrestin recruitment, internalization, and recycling of this particular receptor, which have proved useful in our hands for dissecting its downward trafficking and fate following agonist stimulation.
Studium Consortium for Research and Training in Reproductive Sciences (sCORTS), Tours, France. aulloaa@unam.mx
Alfredo
A
Ulloa-Aguirre
James A
JA
Dias
George
G
Bousfield
Ilpo
I
Huhtaniemi
Eric
E
Reiter
D000818:Animals; D019390:Arrestins / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D009154:Mutation; D010766:Phosphorylation; D055503:Protein Multimerization; D021381:Protein Transport; D011962:Receptors, FSH / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D000071557:beta-Arrestins
D019390:Arrestins; D011962:Receptors, FSH; D000071557:beta-Arrestins; D005640:Follicle Stimulating Hormone