A hybrid assembly by encapsulation of human cells within mineralised beads for cell therapy.
BACKGROUND
The design of new technologies for treatment of human disorders such as protein deficiencies is a complex and difficult task. Particularly, the construction of artificial organs, based on the immunoisolation of protein-secreting cells, requires the use of suitable materials which have to be biocompatible with the immunoisolated cells and avoid any inappropriate host response.


RESULTS
This work investigates the in vivo behavior of mechanically resistant hybrid beads which can be considered as a model for artificial organ for cell therapy. This hybrid system was designed and fabricated via the encapsulation of living cells (HepG2) within alginate-silica composites. Two types of beads (alginate-silica hybrid (AS) or alginate/silica hybrid subsequently covered by an external layer of pure alginate (ASA)), with or without HepG2 cells, were implanted into several female Wistar rats. After four weeks, the potential inflammatory local response that might be due to the presence of materials was studied by histochemistry. The results showed that the performance of ASA beads was quite promising compared to AS beads, where less abnormal rat behaviour and less inflammatory cells in histological sections were observed in the case of ASA beads.


CONCLUSIONS
The current study highlights that alginate-silica composite materials coated with an extra-alginate shell offer much promise in the development of robust implantation devices and artificial organs.
Laboratory of Inorganic Materials Chemistry, The University of Namur, FUNDP, Namur, Belgium.
Philippe
P
Dandoy
Christophe F
CF
Meunier
Grégory
G
Leroux
Virginie
V
Voisin
Laetitia
L
Giordano
Nathalie
N
Caron
Carine
C
Michiels
Bao-Lian
BL
Su
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism; D064987:Cell- and Tissue-Based Therapy; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008264:Macrophages / Q000166:cytology; D008863:Microspheres; D018482:Muscle, Skeletal / Q000378:metabolism; D051381:Rats; D058428:Silica Gel / Q000737:chemistry*
D000464:Alginates; D006603:Hexuronic Acids; D058428:Silica Gel; D020723:Glucuronic Acid