Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy.
Obesity, an ongoing significant public health problem, is a part of complex disease characterized as metabolic syndrome. Medaka and zebrafish are useful aquatic experimental animals widely used in the field of toxicology and environmental health sciences and as a human disease models. In medaka, simple feeding of a high fat diet (HFD) can induce body weight gain, excessive accumulation of visceral adipose tissue, hyperglycemia, hyperlipidemia, and steatohepatitis, which mimics human metabolic syndrome. In the present study, to explore the possibility that the adult medaka fed with HFD (HFD-medaka) can be used as an animal model for human metabolic syndrome-associated glomerular disease, including obesity-related glomerulopathy (ORG), we analyzed structural alterations and protein expression in the mesonephric kidney of HFD-medaka. We found that the histopathology was consistent with glomerulomegaly accompanied by the dilation of glomerular capillaries and proliferative expansion of the mesangium, a condition partially comparable to human ORG. Moreover, expressions of several kinds of kidney disease-related proteins (such as MYH9, SM22α) were significantly elevated. Thus, the HFD-medaka has a high potential as an animal model useful for exploring the mechanism underling human ORG.
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Koichiro
K
Ichimura
Yusuke
Y
Kawashima
Tomomi
T
Nakamura
Rebecca
R
Powell
Yuya
Y
Hidoh
Shuji
S
Terai
Isao
I
Sakaida
Yoshio
Y
Kodera
Takashi
T
Tsuji
Jian-Xing
JX
Ma
Tatsuo
T
Sakai
Hiroyuki
H
Matsumoto
Tomoko
T
Obara
D000818:Animals; D001786:Blood Glucose; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D005921:Glomerulonephritis / Q000097:blood / Q000209:etiology / Q000473:pathology*; D006801:Humans; D007678:Kidney Glomerulus / Q000473:pathology*; D024821:Metabolic Syndrome / Q000150:complications*; D008840:Microfilament Proteins / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D009765:Obesity / Q000150:complications*; D009929:Organ Size; D009990:Oryzias / Q000097:blood / Q000378:metabolism*
D001786:Blood Glucose; D008840:Microfilament Proteins; D009124:Muscle Proteins; C051615:transgelin; D018995:Myosin Heavy Chains