[Effects of the renal blood flow at different levels by transcutaneous electrical acupoint stimulation combined general anesthesia induced controlled hypotension].
OBJECTIVE
To observe the intervention of transcutaneous electrical acupoint stimulation (TEAS) on the renal blood flow at different levels of mean arterial pressure (MAP) in controlled hypotension.


METHODS
Forty-two male beagle dogs were randomly divided into seven groups, i. e., the general anesthesia group, the 50% controlled group, the 40% controlled group, the 30% controlled group, the 50% experimental group, the 40% experimental group, and the 30% experimental group, 6 in each group. Beagles in the general anesthesia group were not treated with controlled hypotension, and the target MAP was achieved in those of the rest groups and maintained for 60 min. In the experimental groups, TEAS was applied to bilateral Hegu (LI4), Zusanli (ST36), Sanyinjiao (SP6), and Quchi (LI11) at 2/100 Hz with the stimulation strength of (4 +/- 1) mA starting from the stability of their physiological conditions to 60 min of maintaining the target MAP level. The changes of the renal blood flow were monitored at different time points using laser Doppler.


RESULTS
From starting pressure control to the target MAP level, the renal blood flow was significantly lower in the 30% controlled group than in the general anesthesia group and the basic level of the same group (P < 0.05), while there was no obvious change in the 30% experimental group. In maintaining the blood pressure, the renal blood flow was significantly lower in the 50% controlled group, the 40% controlled group, the 30% controlled group, and the 30% experimental group than in the general anesthesia group (P < 0.05), while there was no obvious change in the 50% experimental group or the 40% experimental group. By the end of blood pressure recovery, the renal blood flow restored to the basic level in the 50% controlled group, the 50% experimental group, and the 40% experimental group (P > 0.05), while it was not restored to the basic level in the 40% controlled group, the 30% controlled group, and the 30% experimental group (P < 0.05).


CONCLUSIONS
TEAS combined general anesthesia in controlled hypotension could effectively improve the renal blood flow, thus protecting the kidney.
Lab for Acupuncture Neurobiology, Third Clinical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou. fangjianqiao7532@163.com
Jian-Qiao
JQ
Fang
Le-Le
LL
Zhang
Xiao-Mei
XM
Shao
D015669:Acupuncture Points; D000768:Anesthesia, General / Q000379:methods*; D000818:Animals; D004285:Dogs; D007023:Hypotension, Controlled / Q000379:methods*; D008297:Male; D012079:Renal Circulation; D004561:Transcutaneous Electric Nerve Stimulation