Selective fluorescence detection of small-molecule-binding proteins by using a dual photoaffinity labeling system.
PHOTO OPPORTUNITY: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588, Japan. sakuraik@cc.tuat.ac.jp
Kaori
K
Sakurai
Rika
R
Yamada
Ayumi
A
Okada
Masaki
M
Tawa
Shimpei
S
Ozawa
Maia
M
Inoue
D001665:Binding Sites; D024402:Carbonic Anhydrase II / Q000032:analysis* / Q000378:metabolism; D057930:Click Chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D019920:Photoaffinity Labels / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D054852:Small Molecule Libraries / Q000032:analysis* / Q000378:metabolism
D005456:Fluorescent Dyes; D019920:Photoaffinity Labels; D054852:Small Molecule Libraries; D024402:Carbonic Anhydrase II