Identification of common mechanisms by which human and mouse cytomegalovirus seven-transmembrane receptor homologues contribute to in vivo phenotypes in a mouse model.
The mouse cytomegalovirus chemokine receptor homologue (CKR) M33 is required for salivary gland tropism and efficient reactivation from latency, phenotypes partially rescued by the human cytomegalovirus CKR US28. Herein, we demonstrate that complementation of salivary gland tropism is mediated predominantly by G protein-dependent signaling conserved with that of M33; in contrast, both G protein-dependent and -independent pathways contribute to the latency phenotypes. A novel M33-dependent replication phenotype in cultured bone marrow macrophages is also described.
University of Queensland, Clinical Medical Virology Centre & Royal Children's Hospital, Sir Albert Sakzewski Virus Research Centre, Brisbane, Queensland, Australia.
Helen E
HE
Farrell
Alexander M
AM
Abraham
Rhonda D
RD
Cardin
Ann-Sofie
AS
Mølleskov-Jensen
Mette M
MM
Rosenkilde
Nicholas
N
Davis-Poynter
D000704:Analysis of Variance; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003587:Cytomegalovirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017830:Octoxynol; D010641:Phenotype; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000821:virology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D056189:Viral Tropism / Q000502:physiology*; D014775:Virus Activation / Q000502:physiology*; D017735:Virus Latency / Q000502:physiology
D019707:Receptors, Chemokine; D043562:Receptors, G-Protein-Coupled; C411191:US28 receptor, Cytomegalovirus; D014764:Viral Proteins; C450520:seven-transmembrane G-protein-coupled receptor; D017830:Octoxynol; D008156:Luciferases