Effects of two low-shrinkage composites on dental stem cells (viability, cell damaged or apoptosis and mesenchymal markers expression).
To investigate the effects of new two low-shrinkage composites SDR(®) and Venus(®)Bulk Fill on the cell viability, cellular damage and expression of mesenchymal markers on dental stem cells. Specimens from two low-shrinkage composites were eluted with culture medium for 24 h. After 24 h of incubation, cytotoxicity of elutes were evaluated by MTT assay; apoptosis was determined using the DNA-specific fluorochrome Hoechst 33342 and the mesenchymal stem cells markers expression was analyzed by immunofluorescence staining. After 24 h of cell exposure to each extract media, dental stem cells expressed MSCs markers. The interaction among the material and cell line was not significantly correlated [F(1,60) = 2.251, P = 0.39], whereas statistically significant differences among cells lines were observed [F(1,60) = 9.157, P = 0.004], being dental pulp stem cells more resistant that periodontal ligament stem cells. Also, we did not find any significant effect between the tested materials [F(1,60) = 0.090, P = 0.765]. Furthermore, a very low proportion of exposed cells showed condensed or fragmented nuclei, typical of apoptotic cells at 24 h. The results suggest that SDR(®) and Venus(®) Bulk fill and should be considered when selecting an appropriate resin-based dental restorative material.
Cell Therapy Unit, Universitary Hospital Virgen de la Arrixaca, Faculty of Medicine, University of Murcia, Murcia, Spain. fcojavier@um.es
F J
FJ
Rodríguez-Lozano
I
I
Serrano-Belmonte
J C
JC
Pérez Calvo
M T
MT
Coronado-Parra
A
A
Bernabeu-Esclapez
J M
JM
Moraleda
D017209:Apoptosis; D015415:Biomarkers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014070:Tooth / Q000166:cytology*
D015415:Biomarkers