Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus.
In the cricket Gryllus bimaculatus, missing distal parts of the amputated leg are regenerated from the blastema, a population of dedifferentiated proliferating cells that forms at the distal tip of the leg stump. To identify molecules involved in blastema formation, comparative transcriptome analysis was performed between regenerating and normal unamputated legs. Components of JAK/STAT signalling were upregulated more than twofold in regenerating legs. To verify their involvement, Gryllus homologues of the interleukin receptor Domeless (Gb'dome), the Janus kinase Hopscotch (Gb'hop) and the transcription factor STAT (Gb'Stat) were cloned, and RNAi was performed against these genes. Gb'dome(RNAi), Gb'hop(RNAi) and Gb'Stat(RNAi) crickets showed defects in leg regeneration. Blastema expression of Gb'cyclinE was decreased in the Gb'Stat(RNAi) cricket compared with that in the control. Hyperproliferation of blastema cells caused by Gb'fat(RNAi) or Gb'warts(RNAi) was suppressed by RNAi against Gb'Stat. The results suggest that JAK/STAT signalling regulates blastema cell proliferation during leg regeneration.
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama city, Okayama, 700-8530, Japan.
Tetsuya
T
Bando
Yoshiyasu
Y
Ishimaru
Takuro
T
Kida
Yoshimasa
Y
Hamada
Yuji
Y
Matsuoka
Taro
T
Nakamura
Hideyo
H
Ohuchi
Sumihare
S
Noji
Taro
T
Mito
D000818:Animals; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D006135:Gryllidae / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D053612:Janus Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D035002:Lower Extremity / Q000502:physiology*; D012313:RNA / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000235:genetics* / Q000502:physiology; D050791:STAT Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017423:Sequence Analysis, RNA / Q000379:methods; D013223:Statistics as Topic / Q000379:methods; D059467:Transcriptome / Q000235:genetics / Q000502:physiology; D054928:Validation Studies as Topic
D050791:STAT Transcription Factors; D012313:RNA; D053612:Janus Kinases