Effect of IR laser on myoblasts: a proteomic study.
Laser therapy is used in physical medicine and rehabilitation to accelerate muscle recovery and in sports medicine to prevent damages produced by metabolic disturbances and inflammatory reactions after heavy exercise. The aim of this research was to get insight into possible benefits deriving from the application of an advanced IR laser system to counteract deficits of muscle energy metabolism and stimulate the recovery of hypotrophic tissue. We studied the effect of IR laser treatment on proliferation, differentiation, cytoskeleton organization and global protein expression in C2C12 myoblasts. We found that laser treatment induced a decrease in the cell proliferation rate without affecting cell viability, while leading to cytoskeletal rearrangement and expression of the early differentiation marker MyoD. The differential proteome analysis revealed the up-regulation and/or modulation of many proteins known to be involved in cell cycle regulation, cytoskeleton organization and differentiation.
ASAcampus Joint Laboratory, ASA Res. Div., Dept. Clinical Physiopathology, University of Florence, Italy.
Monica
M
Monici
Francesca
F
Cialdai
Francesco
F
Ranaldi
Paolo
P
Paoli
Francesca
F
Boscaro
Gloriano
G
Moneti
Anna
A
Caselli
D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D003599:Cytoskeleton; D004734:Energy Metabolism / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects; D007259:Infrared Rays; D053685:Laser Therapy; D007834:Lasers; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D017570:MyoD Protein / Q000378:metabolism* / Q000528:radiation effects; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D040901:Proteomics; D015854:Up-Regulation
D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein