Display of Clostridium cellulovorans xylose isomerase on the cell surface of Saccharomyces cerevisiae and its direct application to xylose fermentation.
Xylose isomerase (XI) is a key enzyme in the conversion of D-xylose, which is a major component of lignocellulosic biomass, to D-xylulose. Genomic analysis of the bacterium Clostridium cellulovorans revealed the presence of XI-related genes. In this study, XI derived from C. cellulovorans was produced and displayed using the yeast cell-surface display system, and the xylose assimilation and fermentation properties of this XI-displaying yeast were examined. XI-displaying yeast grew well in medium containing xylose as the sole carbon source and directly produced ethanol from xylose under anaerobic conditions.
Div. of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502, Japan.
Miki
M
Ota
Hiroshi
H
Sakuragi
Hironobu
H
Morisaka
Kouichi
K
Kuroda
Hideo
H
Miyake
Yutaka
Y
Tamaru
Mitsuyoshi
M
Ueda
D019747:Aldose-Ketose Isomerases / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology* / Q000235:genetics; D046974:Clostridium cellulovorans / Q000201:enzymology* / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D015870:Gene Expression; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
D001426:Bacterial Proteins; D000431:Ethanol; D014994:Xylose; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase