A rhodamine based fluorescent probe for Hg2+ and its application to cellular imaging.
A new rhodamine-based fluorescent probe (Rh-F) for detection of Hg(2+) ions was synthesized, which could bind Hg(2+) in aqueous ethanol (7:3, v/v) at pH 7.0 with detectable change in color and fluorescence. The response is based on a ring opening reaction and formation of a 1:1 complex, while ring-opening process of spirolactam enables large fluorescent enhancement and colorimetric change upon the addition of Hg(2+). The response is reversible and linear in the range between 200nM and 1000nM, with a detection limit of 4.2nM. Selectivity and competition experiments with various other metal ion revealed that Rh-F possesses highly selective fluorescent response to Hg(2+). Furthermore, the probe was successfully applied to fluorescent imaging of Hg(2+) in L-929 cells confirm that Rh-F can be used as a fluorescent probe for monitoring Hg(2+) in living cells.
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Key Lab of Fiber Modification & Functional Fiber of Tianjin, Tianjin Polytechnic University, Binshui West Road 399, Tianjin 300387, PR China.
Fanyong
F
Yan
Donglei
D
Cao
Ning
N
Yang
Meng
M
Wang
Linfeng
L
Dai
Chuying
C
Li
Li
L
Chen
D000818:Animals; D002413:Cations, Divalent / Q000032:analysis; D002460:Cell Line; D005347:Fibroblasts / Q000737:chemistry* / Q000166:cytology; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D012235:Rhodamines; D008628:Mercury