Effects of soy peptone on the inoculum preparation of Streptococcus zooepidemicus for production of hyaluronic acid.
Soy peptone (SP) was studied as nutrient source in replacement of the conventional media as Brain-Heart Infusion (BHI) and sheep blood in the first seed culture medium in Petri plates of Streptococcus zooepidemicus. This substitution, aimed at meeting the claim of the pharmaceutical and cosmetics industries, for the removal of animal sources of the culture media used in obtaining their products for safety reasons. The animal sources were used as a control. The effects of this substitution were studied in fermentations carried out at 37°C and 150rpm in 250mL Erlenmeyer flasks containing 100mL culture medium containing glucose and SP only. The replacement of animal nutrient sources by SP to about twice the BHI concentration did not alter the amount of the produced HA, or caused deviations in the metabolism of the microorganism in favor of HA to the detriment of cell growth.
University of Campinas - UNICAMP, Laboratory for Development of Biotechnological Processes, School of Chemical Engineering, Av. Albert Einstein 500, 13083-852 Campinas-SP, Brazil.
Leandro Junqueira
LJ
Benedini
Maria Helena Andrade
MH
Santana
D000818:Animals; D049109:Cell Proliferation; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism*; D010461:Peptones / Q000378:metabolism*; D030262:Soybean Proteins / Q000378:metabolism*; D018502:Streptococcus equi / Q000378:metabolism*
D010461:Peptones; D030262:Soybean Proteins; D006820:Hyaluronic Acid; D005947:Glucose