New synthesis method for 4-MAPBA monomer and using for the recognition of IgM and mannose with MIP-based QCM sensors.
Quartz crystal microbalance (QCM) sensors coated with molecularly imprinted polymers (MIP) have been developed for the recognition of immunoglobulin M (IgM) and mannose. In this method, methacryloylamidophenylboronic acid (MAPBA) was used as a monomer and mannose was used as a template. For this purpose, initially, QCM electrodes were modified with 2-propene-1-thiol to form mannose-binding regions on the QCM sensor surface. In the second step, the methacryloylamidophenylboronic acid-mannose [MAPBA-mannose], pre-organized monomer system, was prepared using the MAPBA monomer. Then, a molecularly imprinted film was coated on to the QCM electrode surface under UV light using ethylene glycol dimethacrylate (EDMA), and azobisisobutyronitrile (AIBN) as a cross-linking agent and an initiator, respectively. The mannose can be simultaneously bound to MAPBA and fitted into the shape-selective cavities. The binding affinity of the mannose-imprinted sensors was investigated using the Langmuir isotherm. The mannose-imprinted QCM electrodes have shown homogeneous binding sites for mannose (K(a): 3.3 × 10(4) M(-1)) and heterogeneous binding sites for IgM (K(a1): 1.0 × 10(4) M(-1); K(a2): 3.3 × 10(3) M(-1)).
Anadolu University, Faculty of Science, Department of Chemistry, Yunus Emre Kampüsü, 26470 Eskişehir, Turkey.
Sibel Emir
SE
Diltemiz
Deniz
D
Hür
Rüstem
R
Keçili
Arzu
A
Ersöz
Rıdvan
R
Say
D001897:Boronic Acids / Q000737:chemistry; D004566:Electrodes; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008358:Mannose / Q000032:analysis*; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry
D001897:Boronic Acids; D007075:Immunoglobulin M; D008689:Methacrylates; D013438:Sulfhydryl Compounds; D008358:Mannose