Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecal valve and creation of artificial sphincter.
OBJECTIVE
To analyze, through molecular biology, the diversity of the intestinal microbiota before and after resection of the ileocecal junction and reconstruction of intestinal transit with and without the creation of a neosphincter.


METHODS
Fourteen Wistar rats were divided into two groups: Group A (n = 7), submitted to resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis; and Group B (n = 7) with resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis followed by construction of an artificial sphincter. Intraluminal contents were collected from both groups. The animals were reoperated 20 days after the first procedure, with new collection of intraluminal contents of the ileum and colon. From the samples collected, DNA was extracted for PCR-DGGE. The electrophoretic banding patterns generated in the reaction were analyzed for similarities and diversities of the microbiota.


RESULTS
The diversity of microorganisms was larger and in more samples when collected from the ileum than from the colon. The group with the neosphincter showed the highest variation in the colon, from 2.11 to 2.93. In three animals from each group was established comparing the similarity and not resembled the control.


CONCLUSIONS
ileocecal resection led to changes in ileal microbiota and, with the creation of new sphincter, the changes were even greater.
Post-Graduate Program in Applied Sciences to Surgery and Ophthalmology, Faculty of Medicine, Federal University of MinasGerais-MG, Brazil. barra.angela@oi.com.br
Angela Aparecida
AA
Barra
Alcino Lázaro da
AL
Silva
Cícero de Lima
Cde L
Rena
Maria Cristina Vasconcelos
MC
Furtado
Alessandra Barbosa
AB
Ferreira
Marcelo
M
Nagen
Fernanda de Souza
Fde S
Freitas
D000818:Animals; D007080:Ileocecal Valve / Q000382:microbiology* / Q000601:surgery*; D007422:Intestines / Q000382:microbiology; D008297:Male; D064307:Microbiota; D051381:Rats; D017208:Rats, Wistar; D016741:Urinary Sphincter, Artificial