[Novel hybrid inhibitors of the phage T7 RNA polymerase: synthesis, docking and screening in vitro].
A number of new hybrid heteroaromatic compounds, consisting of tricyclic fragments (acridone, thioxanthone and phenazine) and bicyclic fragments (benzimidazole, benzothiazole and benzoxazole) were synthesized using the method, developed by the authors. As a result of screening against the transcription model system of the phage T7 DNA-dependent RNA polymerase three effective inhibitors of the RNA syntheses with the IC50 value of 8.9, 5.7 and 19.8 microM were detected. To cast light on the mode of interaction between the synthesized compounds and the target, the molecular docking was applied to the model pocket of the phage T7 RNA polymerase transcription complex. It was established that these ligands form networks of H-bonds with residues of the pocket conservative amino acids and pi-interaction with the Mg2+ ion. A planar geometry of the hybrid molecules, realized due to the intramolecular H-bonds, proved to be an important structural feature, which correlates with an efficacious inhibitory activity.
V H
VH
Kostina
L H
LH
Pal'chykovs'ka
M O
MO
Platonov
O V
OV
Vasyl'chenko
N A
NA
Lysenko
I V
IV
Alekseeva
D054831:Acridones / Q000737:chemistry; D017123:Bacteriophage T7 / Q000737:chemistry / Q000235:genetics; D001562:Benzimidazoles / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D001583:Benzoxazoles / Q000737:chemistry; D003198:Computer Simulation; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000138:chemical synthesis*; D006860:Hydrogen Bonding; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D010619:Phenazines / Q000737:chemistry; D021281:Quantitative Structure-Activity Relationship; D012367:RNA, Viral / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D012996:Solutions; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism
D054831:Acridones; D001562:Benzimidazoles; D052160:Benzothiazoles; D001583:Benzoxazoles; D004791:Enzyme Inhibitors; D010619:Phenazines; D012367:RNA, Viral; D012996:Solutions; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium