Enzymatic production and characterization of manno-oligosaccharides from Gleditsia sinensis galactomannan gum.
Enzymatic hydrolysis of Gleditsia sinensis gum was performed to produce manno-oligosaccharides having functional applications as dietary fiber and prebiotics. The optimum hydrolysis conditions, including enzyme loading, temperature and time, from response surface methodology were 8.1 U/g, 57.4 °C and 34.1 h, respectively. The yield of DP 1-5 oligosaccharides was 75.9% (29.1 g/L). The Michaelis-Menten kinetics and molecular weight distribution were determined. The obtained oligosaccharides were further separated by HPLC and SEC, and the galactose distribution of G. sinensis gum was elucidated. Results indicated that G. sinensis gum has potential to produce value-added oligosaccharides in food industries.
Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
Hong-Lei
HL
Jian
Li-Wei
LW
Zhu
Wei-Ming
WM
Zhang
Da-Feng
DF
Sun
Jian-Xin
JX
Jiang
D004043:Dietary Fiber; D005690:Galactose / Q000031:analogs & derivatives; D031501:Gleditsia / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D008351:Mannans / Q000737:chemistry*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D053149:Plant Gums / Q000737:chemistry*; D056692:Prebiotics; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D014783:Viscosity
D004043:Dietary Fiber; D008351:Mannans; D009844:Oligosaccharides; D053149:Plant Gums; D056692:Prebiotics; D012996:Solutions; C012990:galactomannan; D005690:Galactose