Inhibition of enterovirus 71 infections and viral IRES activity by Fructus gardeniae and geniposide.
Fructus gardeniae has long been used by traditional Chinese medical practitioners for its anti-inflammatory, anti-oxidant, anti-tumor and anti-hyperlipidemic characteristics. Here we describe our finding that F. gardeniae greatly reduces anti-enterovirus 71 (EV71) activity, resulting in significant decreases in EV71 virus yields, EV71 infections, and internal ribosome entry site activity. We also found that geniposide, a primary F. gardeniae component, inhibited both EV71 replication and viral IRES activity. Our data suggest the presence of a mechanism that blocks viral protein translation. According to our findings, F. gardeniae and geniposide deserve a closer look as potential chemopreventive agents against EV71 infections.
Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, ROC. yjlin.kath@gmail.com
Ying-Ju
YJ
Lin
Chien-Chen
CC
Lai
Chih-Ho
CH
Lai
Shih-Che
SC
Sue
Cheng-Wen
CW
Lin
Chien-Hui
CH
Hung
Ting-Hsu
TH
Lin
Wei-Yi
WY
Hsu
Shao-Mei
SM
Huang
Yi-Lin
YL
Hung
Ni
N
Tien
Xiang
X
Liu
Chao-Ling
CL
Chen
Fuu-Jen
FJ
Tsai
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D029821:Enterovirus A, Human / Q000187:drug effects*; D004769:Enterovirus Infections / Q000188:drug therapy* / Q000821:virology; D032062:Gardenia / Q000737:chemistry*; D006801:Humans; D039823:Iridoids / Q000737:chemistry / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000187:drug effects / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D039823:Iridoids; D012367:RNA, Viral; C007835:geniposide