Mouse renal 4T1 cell engraftment as a model to study the influence of hypoxia in breast cancer progression.
OBJECTIVE
To develop a mouse model to study the influence of hypoxia in breast cancer progression and metastasis.


METHODS
The 4T1 cell line was used to engraft the kidneys of female BALB/c mice. Placing an aneurysm clip on the kidney hilum, hypoxia can be directed to tumor site. Histological evaluation was used to analyze the morphological changes induced by ischemia in kidney cortex, and to verify the metastatic potential.


RESULTS
4T1 cells can be engrafted into the renal cortex and the renal ischemia caused by using a clip to clamp the renal hilum induces hypoxia at the tumor site. This procedure maintains the ability of 4T1 cells to metastasize. In fact, our preliminary results showed that tumor hypoxia precipitates the metastatic dissemination of tumor cells. After 14 days of engraftment, lung metastases were observed only in mice that were subjected to tumor hypoxia.


CONCLUSIONS
This model can help us to understand how low oxygen tension mediates hypoxia-induced proteomic and genomic changes in breast cancer.
Breast Disease Unit, Department of Gynecology and Obstetrics, Ribeirao Preto School of Medicine, USP, Sao Paulo, Brazil.
Heriton Marcelo Ribeiro
HM
Antonio
Larissa Raquel Mouro
LR
Mandarano
Alan Augusto
AA
Coelho
Marcelo Guimarães
MG
Tiezzi
Jurandyr Moreira de
JM
Andrade
Daniel Guimarães
DG
Tiezzi
D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D007668:Kidney / Q000098:blood supply / Q000473:pathology*; D008175:Lung Neoplasms / Q000556:secondary*; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009368:Neoplasm Transplantation