Morphological evidences of Bio-Oss® colonization by CD44-positive cells.
OBJECTIVE
Our objectives were to examine vascular and cellular colonization in anorganic bovine bone (ABB) after 6 months of healing in human maxillary sinus augmentation grafts.


METHODS
Fifty unilateral maxillary sinus augmentation procedures were performed on 50 consecutive patients. Bone cores were obtained through the implant receptor sites 6 months later and evaluated with morphological image analysis and immunohistochemical techniques.


RESULTS
Image analysis revealed a mean of 35.44 ± 16% vital bone, 31.66 ± 15% non-mineralized tissue, and 32.72 ± 25% remnant ABB particles. In our patients, neovascularization in 46.3% of cases was demonstrated within ABB particles 6 months after sinus floor augmentation surgery. Neovascularization of ABB particles was inversely related to age, directly to osteoclast number per mm², and not influenced by habits or disease. CD44-positive cells colonization was found in 74% of cases. An important correlation was found regarding CD44 expression and number of vessel in ABB particles (r = 0.624 P < 0.001, Pearson). Osteopontin expression was detected on the interstitial boundary of bone with ABB particles and within the osteocyte lacunae and bone canaliculi and was relationship with presence of CD44-positive cells inside ABB particles (r = 0.388, P = 0.046, Pearson).


CONCLUSIONS
In conclusion, images compatible with osteone colonized by osteocytes CD44 positive and neovascularization in ABB particles were observed after 6 months of graft maturation. These biologic events have not previously been morphologically and immunohistochemically documented.
Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, Granada, Spain.
Pablo
P
Galindo-Moreno
Department of Traumatology and Orthopedic Surgery, San Cecilio Clinical Hospital, University of Granada, Granada, Spain.
Pedro
P
Hernández-Cortés
San Cecilio Clinical Hospital, University of Granada University of Granada, Granada, Spain.
José
J
Aneiros-Fernández
San Cecilio Clinical Hospital, University of Granada University of Granada, Granada, Spain.
Miguel
M
Camara
Periodontology Department, School of Dentistry, University of Granada, Granada, Spain.
Francisco
F
Mesa
Department of Periodontology, Columbia University, New York, NY, USA.
Stephen
S
Wallace
Pathology Department, School of Medicine, and IBIMER, University of Granada, Granada, Spain.
Francisco
F
O'Valle
D000328:Adult; D000368:Aged; D000818:Animals; D001706:Biopsy; D002417:Cattle; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D008903:Minerals / Q000494:pharmacology*; D010011:Osteocytes / Q000378:metabolism*; D053495:Osteopontin / Q000378:metabolism; D059546:Sinus Floor Augmentation / Q000379:methods*
C077540:Bio-Oss; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D008903:Minerals; D053495:Osteopontin
CD44; anorganic bovine bone; bone remodeling; intrasinus graft; neovascularization; osteocyte; osteopontin