Chemical synthesis and characterization of epicatechin glucuronides and sulfates: bioanalytical standards for epicatechin metabolite identification.
The monoglucuronides and sulfates of epicatechin, 3'-O-methylepicatechin, and 4'-O-methylepicatechin, respectively, were synthesized as authentic bioanalytical standards. Reversed-phase HPLC methods capable of baseline separation of the glucuronides and sulfates have been developed. Both the epicatechin glucuronides and sulfates were stable in the solid state when stored under ambient conditions and in aqueous solution when stored refrigerated. These results should prove invaluable to the research community as analytical standards as well as in future studies of the biological and pharmacological effects of epicatechin in humans.
Institutes for Pharmaceutical Discovery, 23 Business Park Drive, Branford, Connecticut 06405, United States.
Mingbao
M
Zhang
G Erik
GE
Jagdmann
Michael
M
Van Zandt
Ryan
R
Sheeler
Paul
P
Beckett
Hagen
H
Schroeter
D002392:Catechin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D020719:Glucuronides / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013463:Sulfuric Acid Esters / Q000138:chemical synthesis* / Q000737:chemistry
C424450:3'-O-methylepicatechin; D020719:Glucuronides; D013463:Sulfuric Acid Esters; C523149:epicatechin-glucuronide; D002392:Catechin