Dissecting trafficking and signaling of atypical chemokine receptors.
Atypical chemokine receptors are a distinct subset of chemokine receptors able to modulate immune responses by acting as chemokine decoy/scavengers or transporters. Intracellular trafficking properties sustained by Gαi-independent signaling have emerged as a major determinant of their biological properties, which support continuous uptake, transport, and/or concentration, of the ligands. Here, we are providing methods to study both trafficking and signaling of this class of chemokine receptors focusing on the atypical chemokine receptor D6 that degrades inflammatory CC chemokines.
Humanitas Clinical and Research Center, Rozzano, Italy.
Elena
E
Borroni
Cinzia
C
Cancellieri
Massimo
M
Locati
Raffaella
R
Bonecchi
D000818:Animals; D019742:Chemokines, CC / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods*; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D054447:Receptors, CCR10 / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection / Q000379:methods; D000097628:Chemokine Receptor D6
D019742:Chemokines, CC; D020559:Monomeric GTP-Binding Proteins; D054447:Receptors, CCR10; D000097628:Chemokine Receptor D6