Microbiotas from UC patients display altered metabolism and reduced ability of LAB to colonize mucus.
We compared fecal microbial communities derived either from Ulcerative Colitis (UC) patients in remission (n = 4) or in relapse (n = 4), or from healthy subjects (n = 4). These communities were used for inoculation of a dynamic in vitro gut model, which contained integrated mucin-covered microcosms. We found that the microbiota of the 'mucus' largely differed from that of the 'lumen'. This was partly due to decreased mucus-associated populations of lactic acid producing bacterial populations (LAB), as LAB originating from UC patients had a significantly decreased capacity to colonize the mucin-covered microcosms as compared to those originating from healthy subjects. We found significant differences between the metabolomes of UC patients in relapse and remission, respectively, while the metabolome of patients in remission resembled that of healthy subjects. These novel findings constitute an important contribution to the understanding of the complex etiology of UC.
National Food Institute, Technical University of Denmark, Søborg, Denmark.
Louise Kristine
LK
Vigsnaes
Pieter
P
van den Abbeele
Karolina
K
Sulek
Henrik Lauritz
HL
Frandsen
Casper
C
Steenholdt
Jørn
J
Brynskov
Joan
J
Vermeiren
Tom
T
van de Wiele
Tine Rask
TR
Licht
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D019344:Lactic Acid / Q000378:metabolism; D055442:Metabolome / Q000235:genetics; D054892:Metagenome / Q000502:physiology*; D009077:Mucins / Q000235:genetics / Q000378:metabolism; D009093:Mucus / Q000382:microbiology
D009077:Mucins; D019344:Lactic Acid