Verification of skin autofluorescence values by mass spectrometry in adolescents with type 1 diabetes: brief report.
BACKGROUND
Accumulation of advanced glycation end products (AGEs) in tissues is a major risk factor for diabetes-associated complications. Skin autofluorescence (SAF) values measured by a specific noninvasive approach (AGE Reader; DiagnOptics Technologies B.V., Gröningen, The Netherlands) reflect the overall AGE exposure in skin.


METHODS
In 16 adolescents with type 1 diabetes (age range, 11-18 years) we tested the association between SAF measured with an AGE Reader and the presence of glucuronic acid, 3-indoxyl sulfate, 3-hydroxybutyrate, phenol sulfate, and pentosidine in skin tissue determined with desorption electrospray ionization mass spectrometry (DESI-MS). These compounds are implicated in long-term diabetes complications.


RESULTS
SAF values significantly correlated with levels of compounds measured by DESI-MS (r>0.9 and P<0.001 for each).


CONCLUSIONS
The strong correlation between adolescents' SAF values measured with the AGE Reader and some glycation products measured with DESI-MS indicates that SAF values may be used as surrogate markers of skin exposure to glycemic end products in type 1 diabetes.
Csolnoky Hospital, Veszprém, Hungary. macsaim1@t-online.hu
Emília
E
Mácsai
Zoltán
Z
Takáts
László
L
Derzbach
Anna
A
Körner
Barna
B
Vásárhelyi
D000293:Adolescent; D001786:Blood Glucose / Q000378:metabolism*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000453:epidemiology; D005260:Female; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008297:Male; D013058:Mass Spectrometry; D012867:Skin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D001786:Blood Glucose; D017127:Glycation End Products, Advanced