Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage.
BACKGROUND
Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer.


METHODS
The invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed.


RESULTS
CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis


CONCLUSIONS
CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.
Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Hospital, No,250, Wu-Hsing St,, Taipei 11031, Taiwan.
Cheng-Jeng
CJ
Tai
Tzu-Cheng
TC
Su
Ming-Chung
MC
Jiang
Hung-Chang
HC
Chen
Shing-Chuan
SC
Shen
Woan-Ruoh
WR
Lee
Ching-Fong
CF
Liao
Ying-Chun
YC
Chen
Shu-Hui
SH
Lin
Li-Tzu
LT
Li
Ko-Hung
KH
Shen
Chung-Min
CM
Yeh
Kun-Tu
KT
Yeh
Ching-Hsiao
CH
Lee
Hsin-Yi
HY
Shih
Chun-Chao
CC
Chang
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D045744:Cell Line, Tumor; D029563:Cellular Apoptosis Susceptibility Protein / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D046888:Tissue Array Analysis
D029563:Cellular Apoptosis Susceptibility Protein