Osteoblastic cellular responses on ionically crosslinked chitosan-tripolyphosphate fibrous 3-D mesh scaffolds.
Tripolyphosphate (TPP) crosslinked chitosan (CH)-based fibrous matrices have potential as bioactive scaffolds for bone tissue engineering. This study describes mechanical, biomineralization, and in vitro bone cell growth and differentiation properties of CH-TPP (chitosan-tripolyphosphate) fibrous scaffolds and compared with that of uncrosslinked CH one. The hydrated CH-TPP scaffolds were viscoelastic in nature and their compressive strength was ~2.9 MPa, which is greater than recent polymer experimental bone scaffolds. This improvement in mechanical properties of CH-TPP scaffold may be beneficial toward cancellous bone graft application. Furthermore, CH-TPP fibers supported in vitro biomineralization with phosphate as nucleation site; however, no significant difference in biomineralization morphology was observed with uncrosslinked CH fibers. Interestingly, a significant improvement in cellular responses (>33% increase in cell number based on DNA quantification) was observed when osteoblast like cells were cultured on the CH-TPP scaffolds than that of CH scaffolds without phosphate group. Enhanced osteoblastic differentiation of MG63 cells on CH-TPP scaffolds was also evidenced. Altogether, the results show that the CH-TPP fibrous scaffolds are encouraging for bone tissue engineering.
School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721302, India.
Falguni
F
Pati
Hemjyoti
H
Kalita
Basudam
B
Adhikari
Santanu
S
Dhara
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D019245:Compressive Strength; D003432:Cross-Linking Reagents; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011122:Polyphosphates / Q000737:chemistry; D011506:Proteins / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011122:Polyphosphates; D011506:Proteins; D048271:Chitosan; D000469:Alkaline Phosphatase; C005692:triphosphoric acid
bone tissue engineering; chitosan-tripolyphosphate; ionic crosslinking; osteoblastic differentiation; osteoconduction