New bacterial strain of the genus Ochrobactrum with glyphosate-degrading activity.
Thirty bacterial strains with various abilities to utilize glyphosate as the sole phosphorus source were isolated from farm soils using the glyphosate enrichment cultivation technique. Among them, a strain showing a remarkable glyphosate-degrading activity was identified by biochemical features and 16S rRNA sequence analysis as Ochrobactrum sp. (GDOS). Herbicide (3 mM) degradation was induced by phosphate starvation, and was completed within 60 h. Aminomethylphosphonic acid was detected in the exhausted medium, suggesting glyphosate oxidoreductase as the enzyme responsible for herbicide breakdown. As it grew even in the presence of glyphosate concentrations as high as 200 mM, Ochrobactrum sp. could be used for bioremediation purposes and treatment of heavily contaminated soils.
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Faranak
F
Hadi
Amir
A
Mousavi
Kambiz Akbari
KA
Noghabi
Hadi Ghaderi
HG
Tabar
Ali Hatef
AH
Salmanian
D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism*; D008969:Molecular Sequence Data; D042041:Ochrobactrum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D000097797:Glyphosate
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005998:Glycine; D006540:Herbicides; D012336:RNA, Ribosomal, 16S; D000097797:Glyphosate