microRNA expression profiling and bioinformatic analysis of dengue virus‑infected             peripheral blood mononuclear cells.
Dengue virus (DENV) causes self‑limiting dengue fever (DF), severe dengue             hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It is generally considered             that cytokine storm leads to the increased plasma leakage characteristic of DHF/DSS.             In the present study, peripheral blood mononuclear cells (PBMCs) were isolated             from blood samples of healthy volunteers and infected with DENV serotype 2 (DENV2).             Culture supernatants of DENV2‑infected and -uninfected PBMCs were analyzed using             a human cytokine array. Between a 6‑12 h post‑infection, levels of CCL5, IL‑6             and IL‑8 were markedly elevated, while those of TNF‑α decreased. Total RNA isolated             from these PBMCs was analyzed by human miRNA microarray to identify differentially             expressed microRNAs (miRNAs). Quantitative reverse transcription polymerase chain             reaction was used to validate 11 upregulated and 4 downregulated miRNAs. Sanger             mibase, miRanda and TargetScan were used to identify 261 common predicted genes.             Databases were used to identify homologous sequences on mRNAs of putative target             genes that may be directly bound by the miRNAs identified. We found that cytokines             and epigenetic regulators may be putative target genes of these miRNAs. Using             ingenuity pathway analysis, we noted that canonical pathways, including biological             regulation, may be modulated by these miRNAs.
Institute of Immunology, Zhongshan School of Medicine, Sun Yat‑sen University, Guangzhou 510080, PR China.
Yiming
Y
Qi
Ying
Y
Li
Lin
L
Zhang
Junqi
J
Huang
D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D030541:Databases, Genetic; D003715:Dengue / Q000235:genetics / Q000378:metabolism / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000821:virology; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D019595:Severe Dengue / Q000235:genetics / Q000378:metabolism / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D020506:Untranslated Regions; D015854:Up-Regulation
D018946:Chemokine CCL5; D015850:Interleukin-6; D016209:Interleukin-8; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; D020506:Untranslated Regions