Identification of heat-sensitive QTL derived from common wild rice (Oryza rufipogon Griff.).
Understanding the responses of rice plants to heat-stress is a challenging, yet crucial, endeavor. A set of introgression lines was previously developed using an advanced backcrossing strategy that involved the elite indica cultivar Teqing as the recipient and an accession of common wild rice (Oryza rufipongon Griff.) as the donor. In this study, we evaluated the responses of 90 of these previously developed introgression lines to heat stress. Five quantitative trait loci (QTLs) related to heat response were detected. The phenotypic variances explained by these QTLs ranged from 6.83% to 14.63%, and O. rufipogon-derived alleles at one locus reduced sensitivity to heat. A heat-sensitive introgression line, YIL106, was identified and characterized. Genotypic analysis demonstrated that YIL106 contained four introgressed segments derived from O. rufipongon and two QTLs (qHTS1-1 and qHTS3) related to heat response. Physiological tests, including measurements of chlorophyll content, electrolyte leakage, malondialdehyde content, and soluble sugar content, were consistent with the heat sensitivity observed in YIL106. Ultrastructural analysis of YIL106 mesophyll cells showed that they were severely damaged following heat stress. This suggests that modification of the cell membrane system is a primary response to heat stress in plants. Identification and characterization of the heat-sensitive line YIL106 may facilitate the isolation of genes associated with the response of rice plants to heat stress.
State Key Laboratory of Plant Physiology and Biochemistry, National Center for Evaluation of Agricultural Wild Plants (Rice), Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China.
Dongyang
D
Lei
Lubin
L
Tan
Fengxia
F
Liu
Liyun
L
Chen
Chuanqing
C
Sun
D000483:Alleles; D002241:Carbohydrates / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D006358:Hot Temperature; D008315:Malondialdehyde / Q000032:analysis; D058503:Mesophyll Cells / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D040641:Quantitative Trait Loci; D012639:Seeds / Q000378:metabolism; D013312:Stress, Physiological
D002241:Carbohydrates; D018744:DNA, Plant; D002734:Chlorophyll; D008315:Malondialdehyde