Simultaneous profiling of multiple gene-methylation loci by electrochemical methylation-specific ligase detection reaction.
A novel method of electrochemical methylation-specific ligation detection reaction is first presented for simultaneous evaluation of multiple gene-methylation loci in a single-tube experiment without PCR amplification or restriction enzyme reaction.
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P. R. China. daizong@mail.sysu.edu.cn
Zong
Z
Dai
Ting
T
Cai
Wenyuan
W
Zhu
Xiaoyu
X
Gao
Xiaoyong
X
Zou
D001483:Base Sequence; D019175:DNA Methylation; D004563:Electrochemistry / Q000379:methods*; D056426:Genetic Loci / Q000235:genetics*; D008025:Ligases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D008025:Ligases