Recent advances in bioprocessing application of membrane chromatography.
Compared to traditional chromatography using resins in packed-bed columns, membrane chromatography is a relatively new and immature bioseparation technology based on the integration of membrane filtration and liquid chromatography into a single-stage operation. Over the past decades, advances in membrane chemistry have yielded novel membrane devices with high binding capacities and improved mass transfer properties, significantly increasing the bioprocessing efficiency for purification of biomolecules. Due to the disposable nature, low buffer consumption, and reduced equipment costs, membrane chromatography can significantly reduce downstream bioprocessing costs. In this review, we discuss technological merits and disadvantages associated with membrane chromatography as well as recent bioseparation applications with a particular attention on purification of large biomolecules.
Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
Valerie
V
Orr
Luyang
L
Zhong
Murray
M
Moo-Young
C Perry
CP
Chou
D001709:Biotechnology / Q000379:methods*; D002845:Chromatography / Q000379:methods*; D046911:Macromolecular Substances / Q000302:isolation & purification; D008567:Membranes, Artificial; D008956:Models, Chemical
D046911:Macromolecular Substances; D008567:Membranes, Artificial