Association of reduced glyoxalase 1 activity and painful peripheral diabetic neuropathy in type 1 and 2 diabetes mellitus patients.
OBJECTIVE
The present study was undertaken to investigate the relationship between glyoxalase 1 (Glo1) enzyme activity and painful diabetic neuropathy (DN) in patients with diabetes mellitus.


METHODS
Glo1 activity and biochemical markers were determined in blood samples from 108 patients with type 1 diabetes, 109 patients with type 2 diabetes, and 132 individuals without diabetes as a control. Painful and painless peripheral DN was assessed and multivariate regression analysis was used to determine independent association of Glo1 activity with occurrence of painful DN.


RESULTS
In patients with type 1 and type 2 diabetes mellitus and painful DN compared to patients with painless DN, Glo1 activity was significantly reduced by 12 and 14%, respectively. The increase in Glo1 activity was significantly associated with reduced occurrence of painful DN after adjusting for confounders by multivariate analysis.


CONCLUSIONS
Our results demonstrate for the first time that Glo1 activity is lower in patients with both types of diabetes mellitus who were diagnosed with painful DN. These data support the hypothesis that Glo1 activity modulates the phenotype of DN and warrant further investigation into the role of Glo1 in DN.
Latvian Institute of Organic Synthesis, Laboratory of Pharmaceutical Pharmacology, Riga, Latvia. skapare@biomed.lu.lv
Elina
E
Skapare
Ilze
I
Konrade
Edgars
E
Liepinsh
Ieva
I
Strele
Marina
M
Makrecka
Angelika
A
Bierhaus
Aivars
A
Lejnieks
Valdis
V
Pirags
Maija
M
Dambrova
D000328:Adult; D015415:Biomarkers / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000150:complications* / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000503:physiopathology; D003929:Diabetic Neuropathies / Q000097:blood / Q000201:enzymology* / Q000503:physiopathology; D018450:Disease Progression; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010146:Pain / Q000097:blood / Q000201:enzymology* / Q000503:physiopathology; D010147:Pain Measurement; D010523:Peripheral Nervous System Diseases / Q000097:blood / Q000201:enzymology* / Q000503:physiopathology; D012720:Severity of Illness Index
D015415:Biomarkers; D006442:Glycated Hemoglobin; D007791:Lactoylglutathione Lyase; C517652:hemoglobin A1c protein, human