In vitro modeling of human bocavirus 1 infection of polarized primary human airway epithelia.
Human bocavirus 1 (HBoV1) is an emerging human-pathogenic respiratory virus. We characterized two important features of HBoV1 infection in polarized primary human airway epithelia (HAE). Apical HBoV1 infection of HAE at a low multiplicity of infection causes disruption of the tight junction barrier, loss of cilia, and epithelial cell hypertrophy, which are hallmarks of the airway epithelial damage caused by HBoV1 infection. HBoV1 also infects HAE from the basolateral surface productively, although less efficiently, and this also leads to the characteristic airway epithelial damage.
College of Life Sciences, Central China Normal University, Wuhan, China.
Xuefeng
X
Deng
Ziying
Z
Yan
Yong
Y
Luo
Jian
J
Xu
Fang
F
Cheng
Yi
Y
Li
John F
JF
Engelhardt
Jianming
J
Qiu
D016764:Cell Polarity / Q000502:physiology; D002923:Cilia / Q000473:pathology / Q000821:virology; D017097:Electric Impedance; D005455:Fluorescent Antibody Technique; D056661:Human bocavirus; D006801:Humans; D006984:Hypertrophy / Q000473:pathology / Q000821:virology; D066298:In Vitro Techniques; D008954:Models, Biological; D010322:Parvoviridae Infections / Q000473:pathology*; D020545:Respiratory Mucosa / Q000821:virology*; D019108:Tight Junctions / Q000473:pathology / Q000821:virology