The effect of PCL-TCP scaffold loaded with mesenchymal stem cells on vertical bone augmentation in dog mandible: a preliminary report.
Polycaprolactone-tricalcium phosphate (PCL-TCP), a new composite scaffold, has been shown to facilitate early revascularization and speed up bone regeneration process. The objective of this study was to evaluate the effect of PCL-TCP seeded with mesenchymal stem cells (MSCs) on healing of the vertical bone critical sized defect in dog's mandible. Bone marrow aspirate from dog humerous was cultured and the stemness of the cells was examined by differentiation staining methods and flow cytometric analysis. Third passage subculture cells (5 × 10⁵ cells) were loaded on 20 × 10 × 10 mm³ and incubated for 48 h. The presence of MSCs in the pores was evaluated by scanning electron microscope. Bilateral mandibular premolar teeth were extracted in four dogs and the buccal and lingual bone plates were reduced to make a vertical defect. Cell-loaded scaffolds were fixed in right side and left side received pure PCL-TCP scaffolds as a control side defects. Histomorphometric analysis after 8 weeks of the scaffold implantation showed higher amount of lamellar bone in the test side (48.63%) than control side (17.27%) (p < 0.05).The results suggest that PCL-TCP may be an appropriate scaffold for loading MSCs in bone regeneration.
Department of Oral and Maxillofacial Surgery, Dental Research Center, Research Institute of Dental Sciences, Dental Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran. arashkhojasteh@yahoo.com
Arash
A
Khojasteh
Hossein
H
Behnia
Fatemeh Sadat
FS
Hosseini
Mohammad Mehdi
MM
Dehghan
Pegah
P
Abbasnia
Fatemeh Mashhadi
FM
Abbas
D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry*; D004285:Dogs; D008297:Male; D008334:Mandible / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; C016240:polycaprolactone; C018392:tricalcium phosphate