Development of ultrasound-assisted emulsification microextraction for determination of thiocynate ion in human urine and saliva samples.
Ultrasound-assisted emulsification microextraction (USAE-ME) procedure coupled with UV-vis spectrophotometric measurement has been developed for determination of thiocyanate ion (SCN(-)) in water and biological fluids samples. The method is based on protonation of SCN(-) ions in acidic medium and extraction of thiocyanic acid into fine droplets of chloroform as an extraction solvent contains rhodamine B (RhB). The RhB was protonated in presence of thiocynanic acid to form highly colored ion-pair complex of [thiocynate][RhBH(+)] in chloroform, which used for subsequent spectrophotometric determination of SCN(-) ions. Experimental parameters for both spectrophotometric reaction and USAE-ME procedure have been optimized. Under optimized conditions the calibration curve for SCN(-) showed good linearity in the range of 38.0-870.0ngmL(-1) (R(2)=0.9967). The limit of detection (S/N=3) and preconcentration factor were 5.0ngmL(-1) and 40, respectively. Relative standard deviation for determination of 200ngmL(-1) of SCN(-) was 2.8% (n=5). The proposed method has been successfully applied for determination of SCN(-) ion in tap water, mineral bottled water and human saliva and urine samples with an average recovery of 99.2%.
Faculty of Chemistry, Bu-Ali Sina university, Hamedan, Iran.
Mahdi
M
Hashemi
Seyed Mosayeb
SM
Daryanavard
Sana
S
Abdolhosseini
D000328:Adult; D002498:Centrifugation; D005591:Chemical Fractionation / Q000379:methods*; D002725:Chloroform / Q000737:chemistry; D060766:Drinking Water / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012235:Rhodamines / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D012907:Smoking / Q000378:metabolism / Q000652:urine; D012965:Sodium Chloride / Q000737:chemistry; D013010:Sonication / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013464:Sulfuric Acids / Q000737:chemistry; D013861:Thiocyanates / Q000032:analysis* / Q000737:chemistry / Q000652:urine*
D060766:Drinking Water; D012235:Rhodamines; D013464:Sulfuric Acids; D013861:Thiocyanates; D012965:Sodium Chloride; D002725:Chloroform; C026976:thiocyanic acid; C029773:rhodamine B; C033158:sulfuric acid