Phosphorylation status of Fas-associated death domain protein is associated with biochemical recurrence after radical prostatectomy.
OBJECTIVE
To assess whether the phosphorylated Fas-associated death domain protein (FADD) at 194 serine (p-FADD) is valuable as a marker of biochemical recurrence in hormone-naive patients who had undergone radical prostatectomy.


METHODS
We used radical prostatectomy specimens from 106 patients. None of the patients had received neoadjuvant or adjuvant therapy. The percentage of positive p-FADD cells (nuclear staining) was immunohistochemically evaluated. The correlation between FADD phosphorylation and the clinicopathologic parameters was assessed. The correlation between the biochemical recurrence-free rate and the p-FADD expression level was analyzed using the Kaplan-Meier method.


RESULTS
Overall, 39 patients developed biochemical recurrence. We investigated the expression of p-FADD in 106 patients with prostate cancer using immunohistochemistry. We compared our findings with the clinicopathologic parameters, including the follow-up data. Patients with a greater positive p-FADD rate had a significantly lower biochemical recurrence rate than those with a lower positive p-FADD rate (P < .001). A significant inverse correlation was found between the positive p-FADD rate and the Gleason score.


CONCLUSIONS
A low expression of p-FADD could be a predictor of biochemical recurrence in hormone-naive patients who have undergone radical prostatectomy.
Department of Urology, Nara Medical University, Kashihara, Nara, Japan.
Tomohiro
T
Ikeda
Nobumichi
N
Tanaka
Keiji
K
Shimada
Yoshiaki
Y
Matsumura
Makito
M
Miyake
Satoshi
S
Anai
Atsushi
A
Tomioka
Eijiro
E
Okajima
Akihide
A
Hirayama
Kiyohide
K
Fujimoto
Noboru
N
Konishi
Yoshihiko
Y
Hirao
D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D053200:Fas-Associated Death Domain Protein / Q000378:metabolism*; D006801:Humans; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000378:metabolism*; D010766:Phosphorylation; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000601:surgery*
D015415:Biomarkers; D053200:Fas-Associated Death Domain Protein