Colorimetric detection of metallothioneins using a thymine-rich oligonucleotide-Hg complex and gold nanoparticles.
A simple and sensitive method for label-free, colorimetric detection of metallothioneins (MTs) has been developed by using a thymine (T)-rich oligonucleotide (TRO)-Hg-AuNP system. In this colorimetric strategy, the thiol groups of MTs could interact with mercury from the T-Hg(2+)-T complex to release TRO, resulting in a color change of the system. The response signals linearly correlated with the concentration of MTs over the range of 2.56 × 10(-8) to 3.08 × 10(-7) mol L(-1), and the limit of detection was 7.67 × 10(-9) mol L(-1). The relative standard deviation and the recovery were 2.3-4.8% (n = 11) and 94.2-103.9%, respectively. The proposed method avoids the label and derivatization steps in common methods, allows direct analysis of the samples by the naked eye without costly instruments, and is reliable, inexpensive, and sensitive.
College of Public Health, University of South China, Hengyang 421001, People's Republic of China.
Qiu-Mei
QM
Qian
Yong-Sheng
YS
Wang
Hui-Xian
HX
Yang
Jin-Hua
JH
Xue
Lu
L
Liu
Bin
B
Zhou
Jia-Cheng
JC
Wang
Ji-Cheng
JC
Yin
Yong-Song
YS
Wang
D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008668:Metallothionein / Q000652:urine*; D009841:Oligonucleotides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry; D012680:Sensitivity and Specificity; D013941:Thymine / Q000737:chemistry*
D009841:Oligonucleotides; D009942:Organometallic Compounds; D006046:Gold; D008668:Metallothionein; D008628:Mercury; D013941:Thymine