Anti-obesity effects of galangin, a pancreatic lipase inhibitor in cafeteria diet fed female rats.
BACKGROUND
Alpinia galanga Willd (Zingiberaceae) (AG) is a rhizomatous herb widely cultivated in shady regions of Malaysia, India, Indochina and Indonesia. It is used in southern India as a domestic remedy for the treatment of rheumatoid arthritis, cough, asthma, obesity, diabetes, etc. It was reported to have anti-obesity, hypoglycemic, hypolipidemic and antioxidant properties.


OBJECTIVE
A flavonol glycoside, galangin, was isolated from AG rhizomes. Based on its in vitro pancreatic lipase inhibitory effect, the study was further aimed to clarify whether galangin prevented obesity induced in female rats by feeding cafeteria diet (CD) for 6 weeks.


METHODS
The in vitro pancreatic lipase inhibitory effect of galangin was determined by measuring the release of oleic acid from triolein. For in vivo experiments, female albino rats were fed CD with or without 50 mg/kg galangin for 6 weeks. Body weight and food intake was measured at weekly intervals. On day 42, serum lipids levels were estimated and then the weight of liver and parametrial adipose tissue (PAT) was determined. The liver lipid peroxidation and triglyceride (TG) content was also estimated.


RESULTS
The IC50 value of galangin for pancreatic lipase was 48.20 mg/mL. Galangin produced inhibition of increased body weight, energy intake and PAT weight induced by CD. In addition, galangin produced a significant decrease in serum lipids, liver weight, lipid peroxidation and accumulation of hepatic TGs.


CONCLUSIONS
Galangin present in AG rhizomes produces anti-obesity effects in CD-fed rats; this may be mediated through its pancreatic lipase inhibitory, hypolipidemic and antioxidant activities.
Department of Pharmaceutical Chemistry, KLE University's College of Pharmacy , Belgaum, Karnataka , India. shivkumarmatur@gmail.com
Shiv
S
Kumar
K R
KR
Alagawadi
D032429:Alpinia / Q000737:chemistry*; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification; D004032:Diet / Q000009:adverse effects; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007194:India; D020128:Inhibitory Concentration 50; D008049:Lipase / Q000037:antagonists & inhibitors; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008519:Medicine, Traditional / Q000379:methods; D009765:Obesity / Q000517:prevention & control*; D019301:Oleic Acid / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome; D014280:Triglycerides / Q000378:metabolism; D014304:Triolein / Q000378:metabolism
D019440:Anti-Obesity Agents; D000975:Antioxidants; D004791:Enzyme Inhibitors; D005419:Flavonoids; D008055:Lipids; D014280:Triglycerides; D014304:Triolein; C037032:galangin; D019301:Oleic Acid; D008049:Lipase