The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats.
Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immunohistochemical and biochemical assay in a rat uterine horn model with full thickness injury. Thirty-two female Wistar albino rats were randomised into five groups: Control group, with no intervention; uterine scar group 15days (US15d), uterine scar group 15 days + alpha lipoic acid (ALA) (US15d + ALA), uterine scar group 30 days (US30d) and uterine scar group 30 days + ALA (US30 days + ALA). After uterine incision 100 mg/kg of ALA was administered by oral gavage for either 15 or 30 days. Vascular endothelial growth factor (VEGF) and alpha smooth muscle actin (α-SMA) distribution were evaluated by immunohistochemical methods in tissue and ELISA methods in tissue homogenate. The percentage of α-SMA positive area in US15d + ALA and US30d + ALA groups was significantly higher than US15 and US30d groups. The percentage of VEGF positive area in US15d + ALA group was significantly higher than US15d group and US30d + ALA group was significantly higher than US30d group. Biochemically, α-SMA was significantly higher in the US15d + ALA group when compared to US15d group and higher in US30d + ALA group when compared to US30d group. VEGF was significantly higher in US15d + ALA and US30d + ALA groups when compared to US15 and US30d groups. In conclusion, ALA was found to be effective in enhancing wound healing in uterine full thickness injury.
Department of Histology and Embryology, Dokuz Eylul University School of Medicine, Izmir, Turkey. cilaker6@yahoo.com
Serap Cilaker
SC
Micili
Asli
A
Goker
Oya
O
Sayin
Pinar
P
Akokay
Bekir Uğur
BU
Ergur
D000199:Actins / Q000378:metabolism; D000818:Animals; D002921:Cicatrix / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology; D008063:Thioctic Acid / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000199:Actins; D008063:Thioctic Acid; D042461:Vascular Endothelial Growth Factor A; C498862:Acta2 protein, rat; C467498:vascular endothelial growth factor A, rat