Effects of chronic caffeine intake in a mouse model of amyotrophic lateral sclerosis.
Caffeine is a nonselective adenosine receptor antagonist; chronic consumption has proved protective toward neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. The present study was designed to determine whether caffeine intake affected survival and/or motor performance in a transgenic model of amyotrophic lateral sclerosis (ALS). SOD1(G93A) mice received caffeine through drinking water from 70 days of age until death. Body weight, motor performance and survival were evaluated. Furthermore, the expression of adenosine A(2A) receptors (A(2A) Rs), glial glutamate transporter (GLT1), and glial fibrillar acidic protein (GFAP) were evaluated by Western blotting. The results showed that caffeine intake significantly shortened the survival of SOD1(G93A) mice (log rank test, P = 0.01) and induced a nonsignificant advancing of disease onset. The expression of A(2A) R, GLT1, and GFAP was altered in the spinal cords of ALS mice, but caffeine did not influence their expression in either wild-type or SOD1(G93) mice. These data indicate that adenosine receptors may play an important role in ALS.
Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy. rosa.potenza@iss.it
Rosa Luisa
RL
Potenza
Monica
M
Armida
Antonella
A
Ferrante
Antonella
A
Pèzzola
Alessandra
A
Matteucci
Maria
M
Puopolo
Patrizia
P
Popoli
D000284:Administration, Oral; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D002110:Caffeine / Q000008:administration & dosage*; D004195:Disease Models, Animal; D008136:Longevity / Q000187:drug effects*; D051379:Mice; D008822:Mice, Transgenic; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism; D009048:Motor Skills / Q000187:drug effects*; D043705:Receptor, Adenosine A2A / Q000378:metabolism; D045442:Rotarod Performance Test; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1
D043705:Receptor, Adenosine A2A; D002110:Caffeine; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1