Snail2 promotes osteosarcoma cell motility through remodelling of the actin cytoskeleton and regulates tumor development.
The function of Snail2 in mesenchymal tumors is, to date unknown. Using knockdown and overexpression studies, we show that Snail2 regulates migration and invasion of osteosarcoma cells. Knockdown resulted in significantly decreased motility, remodelling of the actin cytoskeleton, and loss of cellular protrusions. Over-expression increased motility, formation of actin-rich cellular protrusions, and altered expression of some non-canonical Wnt pathway components whilst decreasing expression of the adhesion molecule OB-cadherin. Unexpectedly, knockdown also resulted in significantly smaller tumors in an in vivo CAM assay. Therefore Snail2 may be a potential therapeutic target for clinical intervention of osteosarcoma.
Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK. a.s.sharili@qmul.ac.uk
Amir-Shaya
AS
Sharili
Steve
S
Allen
Ken
K
Smith
Joanna
J
Price
Imelda M
IM
McGonnell
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015820:Cadherins / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D002642:Chick Embryo; D022001:Focal Adhesions / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D034741:RNA, Small Interfering; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000235:genetics; D000071818:Wnt-5a Protein; D000094742:Secreted Frizzled-Related Proteins
D015820:Cadherins; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C109618:SFRP2 protein, human; D000094742:Secreted Frizzled-Related Proteins; C000604407:SNAI1 protein, human; C085389:WNT5A protein, human; C086960:osteoblast cadherin