Whole-genome mapping: a new paradigm in strain-typing technology.
Strain-typing technology in support of outbreak identification and resolution has evolved from phenotypic analysis, such as serology and biotypes, to much-more-robust molecular genetic approaches, such as pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing. Whole-genome mapping (WGM) has been recently applied to subtyping analysis, and it bridges the gap between PFGE (∼20 bands sorted by size) and whole-genome sequencing. WGM utilizes restriction site analysis but arranges 200 to 500 bands in the order they appear on the chromosome. WGM is able to quickly and cost-effectively generate high-resolution, ordered whole-genome maps of bacteria.
Microbiology Technical Services, LLC, Dunwoody, Georgia, USA. jmm8@comcast.net
J Michael
JM
Miller
D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*