Allelic variants in TLR10 gene may influence bilateral affectation and clinical course of Meniere's disease.
Toll-like receptors trigger the innate immune response by activating various cell types such us macrophages and lymphocytes. We genotyped SNV of TLR3, TRL7, TLR8 and TLR10 in 863 Spanish and 150 Italian patients with Meniere's disease (MD) and 1,013 controls by using Taqman assays. Real-Time qPCR was used to measure the expression level of TLR10 in peripheral blood leukocytes. The overall dataset showed that the C allele and the CC genotype of rs11096955 in TLR10 gene were more commonly observed in controls than patients (corrected p = 1 × 10(-3), OR = 0.68 [95 % confidence interval, 0.54-0.84] for CC genotype; corrected p = 1.5 × 10(-5), OR = 0.75 [0.66-0.85] for allele C). Moreover, the CC genotype was more frequent in patients with uni- (19 %) than bilateral sensorineural hearing loss (SNHL) (13 %). Logistic regression demonstrated that the time since the onset of MD, Tumarkin crises, hearing stage and rs11096955 were independent factors influencing the risk of bilateral SNHL. In addition, rs11096955 influenced hearing loss progression in patients with bilateral MD. No change in expression of TLR10 was observed according to CC, CA or AA genotypes. Our data suggest that allelic variants of TLR10 gene may influence the susceptibility and time-course of hearing loss of MD in the European population.
Human DNA Variability Department, Centro de Genómica e Investigación Oncológica, Pfizer/Universidad de Granada/Junta de Andalucía (GENYO), Granada, Spain.
Teresa
T
Requena
Irene
I
Gazquez
Antonia
A
Moreno
Angel
A
Batuecas
Ismael
I
Aran
Andres
A
Soto-Varela
Sofia
S
Santos-Perez
Nicolas
N
Perez
Herminio
H
Perez-Garrigues
Alicia
A
Lopez-Nevot
Eduardo
E
Martin
Ricardo
R
Sanz
Paz
P
Perez
Gabriel
G
Trinidad
Marta E
ME
Alarcon-Riquelme
Roberto
R
Teggi
Laura
L
Zagato
Miguel A
MA
Lopez-Nevot
Jose A
JA
Lopez-Escamez
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D006319:Hearing Loss, Sensorineural / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008575:Meniere Disease / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051201:Toll-Like Receptor 10 / Q000235:genetics*; D051196:Toll-Like Receptor 3 / Q000235:genetics; D051199:Toll-Like Receptor 7 / Q000235:genetics; D051200:Toll-Like Receptor 8 / Q000235:genetics; D044465:White People; D055815:Young Adult
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C475955:TLR10 protein, human; C495344:TLR3 protein, human; C495348:TLR7 protein, human; C495349:TLR8 protein, human; D051201:Toll-Like Receptor 10; D051196:Toll-Like Receptor 3; D051199:Toll-Like Receptor 7; D051200:Toll-Like Receptor 8