Fasting launches CRTC to facilitate long-term memory formation in Drosophila.
Canonical aversive long-term memory (LTM) formation in Drosophila requires multiple spaced trainings, whereas appetitive LTM can be formed after a single training. Appetitive LTM requires fasting prior to training, which increases motivation for food intake. However, we found that fasting facilitated LTM formation in general; aversive LTM formation also occurred after single-cycle training when mild fasting was applied before training. Both fasting-dependent LTM (fLTM) and spaced training-dependent LTM (spLTM) required protein synthesis and cyclic adenosine monophosphate response element-binding protein (CREB) activity. However, spLTM required CREB activity in two neural populations--mushroom body and DAL neurons--whereas fLTM required CREB activity only in mushroom body neurons. fLTM uses the CREB coactivator CRTC, whereas spLTM uses the coactivator CBP. Thus, flies use distinct LTM machinery depending on their hunger state.
Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya, Tokyo 156-0057, Japan. hirano-yk@igakuken.or.jp
Yukinori
Y
Hirano
Tomoko
T
Masuda
Shintaro
S
Naganos
Motomi
M
Matsuno
Kohei
K
Ueno
Tomoyuki
T
Miyashita
Junjiro
J
Horiuchi
Minoru
M
Saitoe
D000818:Animals; D050882:CREB-Binding Protein / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005215:Fasting; D057567:Memory, Long-Term / Q000187:drug effects; D024521:Mushroom Bodies / Q000502:physiology; D009474:Neurons / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism*
C578084:CRTC protein, Drosophila; D017362:Cyclic AMP Response Element-Binding Protein; D029721:Drosophila Proteins; D011500:Protein Synthesis Inhibitors; D014157:Transcription Factors; D003513:Cycloheximide; D050882:CREB-Binding Protein