Safety of and cellular response to segmental bronchoprovocation in allergic asthma.
BACKGROUND
Despite its incorporation into research studies, the safety aspects of segmental allergen bronchoprovocation and differences in cellular response among different allergens have received limited consideration.


METHODS
We performed 87 segmental challenges in 77 allergic asthma subjects. Allergen dose was based on each subject's response to whole lung allergen challenge. Bronchoalveolar lavage was performed at 0 and 48 hours. Safety indicators included spirometry, oxygen saturation, heart rate, and symptoms.


RESULTS
Among subjects challenged with ragweed, cat dander, or house dust mite, there were no differences in safety indicators. Subjects demonstrated a modest oxygen desaturation and tachycardia during the procedure that returned to normal prior to discharge. We observed a modest reduction in forced vital capacity and forced expiratory volume in one second following bronchoscopy. The most common symptoms following the procedure were cough, sore throat and fatigue. Total bronchoalveolar lavage fluid cell numbers increased from 13±4 to 106±108×10(4) per milliliter and eosinophils increased from 1±2 to 44±20 percent, with no significant differences among the three allergens.


CONCLUSIONS
In mild allergic asthma, segmental allergen bronchoprovocation, using individualized doses of aeroallergens, was safe and yielded similar cellular responses.
Allergy, Pulmonary and Critical Care Division, Department of Medicine, University of Wisconsin, Madison, WI, USA.
Loren C
LC
Denlinger
Elizabeth A B
EA
Kelly
Ann M
AM
Dodge
John G
JG
McCartney
Keith C
KC
Meyer
Richard D
RD
Cornwell
Mary Jo
MJ
Jackson
Michael D
MD
Evans
Nizar N
NN
Jarjour
D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000473:pathology* / Q000503:physiopathology; D001985:Bronchial Provocation Tests / Q000009:adverse effects*; D018893:Bronchoalveolar Lavage; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D001999:Bronchoscopy; D002415:Cats; D005260:Female; D005541:Forced Expiratory Volume; D006339:Heart Rate; D006801:Humans; D008297:Male; D010100:Oxygen / Q000378:metabolism; D039981:Pyroglyphidae / Q000276:immunology; D013147:Spirometry; D014797:Vital Capacity; D055815:Young Adult
D000485:Allergens; D010100:Oxygen