The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.
Hepatitis B X-interacting protein (HBXIP) is a novel oncoprotein. In this study, we found that the expression levels of HBXIP were positively associated with those of S-phase kinase-associated protein 2 (Skp2) in clinical breast cancer tissues and cell lines. Moreover, we found that HBXIP was able to stimulate the promoter of Skp2 through binding to the -640/-443 region in Skp2 promoter involving activating E2F transcription factor 1 (E2F1). Skp2 plays crucial roles in HBXIP-enhanced proliferation of breast cancer cells in vitro and in vivo. We conclude that HBXIP up-regulates Skp2 via activating E2F1 to promote proliferation of breast cancer cells.
Department of Cancer Research, Key Laboratory of Molecular Microbiology and Technology of Ministry of Education, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
Fuqiang
F
Xu
Xiaona
X
You
Fabao
F
Liu
Xiaohong
X
Shen
Yuanqing
Y
Yao
Lihong
L
Ye
Xiaodong
X
Zhang
D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D049109:Cell Proliferation; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011401:Promoter Regions, Genetic; D044786:S-Phase Kinase-Associated Proteins / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
D048868:Adaptor Proteins, Signal Transducing; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; C110719:LAMTOR5 protein, human; D044786:S-Phase Kinase-Associated Proteins