MicroRNA-30b-5p is involved in the regulation of cardiac hypertrophy by targeting CaMKIIδ.
BACKGROUND
MicroRNAs (miRNAs) participate in the regulation of cardiac hypertrophy. However, it remains largely unknown as to how miRNAs are integrated into the hypertrophic program. Ca/calmodulin-dependent protein kinase II (CaMKII) is a hypertrophic signaling marker. It is not yet clear which miRNAs can regulate CaMKIIδ.


OBJECTIVE
In this study, we identified which miRNAs could regulate CaMKIIδ and how to regulate CaMKIIδ.


METHODS
Through computational and expression analyses, miR-30b-5p was identified as a candidate regulator of CaMKIIδ. Quantitative expression analysis of hypertrophic models demonstrated significant down-regulation of miR-30b-5p compared with control groups. Luciferase reporter assay showed that miR-30b-5p could significantly inhibit the expression of CaMKIIδ. Moreover, through gain-of-function and loss-of-function approaches, we found miR-30b-5p could negatively regulate the expression of CaMKIIδ and miR-30b-5p was a regulator of cardiac hypertrophy.


CONCLUSIONS
Our study demonstrates that the expression of miR-30b-5p is down-regulated in cardiac hypertrophy, and restoration of its function inhibits the expression of CaMKIIδ, suggesting that miR-30b-5p may act as a hypertrophic suppressor.
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
Juan
J
He
Shan
S
Jiang
Feng-lan
FL
Li
Xue-jiao
XJ
Zhao
Er-fu
EF
Chu
Mei-na
MN
Sun
Ming-zi
MZ
Chen
Hui
H
Li
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D006332:Cardiomegaly / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000473:pathology; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar
D020413:3' Untranslated Regions; C556157:MIRN30 microRNA, rat; D035683:MicroRNAs; D008156:Luciferases; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2