Anti-inflammatory and antimicrobial properties of pyrroloquinazoline alkaloids from Adhatoda vasica Nees.
Adhatoda vasica Nees, Acanthaceae, is well known plant in Ayurveda and Unani medicine. The purpose of this study was to characterize the most bioactive phytochemicals viz., vasicine, vasicinone, vasicine acetate, 2-acetyl benzyl amine, vasicinolone present in the chloroform fraction having anti-inflammatory and antimicrobial activities. The anti-inflammatory activity was tested by using carrageenan and CFA-model induced paw oedema. The antimicrobial activity of isolated compounds was assessed by using the microdilution method. The observed results revealed that vasicine showed most potent anti-inflammatory effects (59.51%) at the dose of 20.0mg/kg at 6h after carrageenan injection and maximum inhibition rate was observed of vasicinone (63.94%) at the dose of 10.0mg/kg at 4 days after CFA injection. The strong antibacterial activity was exhibited by vasicine at 20μg/ml dose against E. coli and also demonstrated maximum antifungal activity against C. albicans at the dose of >55μg/ml. All the five alkaloids demonstrated significant anti-inflammatory and antimicrobial activities.
Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, India. bharatsingh217@gmail.com
Bharat
B
Singh
Ram Avtar
RA
Sharma
D000098:Acetophenones / Q000737:chemistry / Q000302:isolation & purification; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001596:Benzylamines / Q000737:chemistry / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects; D002351:Carrageenan / Q000009:adverse effects; D005591:Chemical Fractionation / Q000379:methods; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004926:Escherichia coli / Q000187:drug effects; D027902:Justicia / Q000737:chemistry*; D008297:Male; D008826:Microbial Sensitivity Tests; D018517:Plant Roots / Q000737:chemistry; D011799:Quinazolines / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
C558632:2-acetyl benzylamine; D000098:Acetophenones; D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000935:Antifungal Agents; D001596:Benzylamines; D011799:Quinazolines; C019592:vasicine; D002351:Carrageenan; C052548:vasicinone