Dissemination of antimicrobial-resistant clones of Salmonella enterica among domestic animals, wild animals, and humans.
Non-typhoidal salmonellosis is an important zoonotic disease caused by Salmonella enterica. This work focuses on the identification of Salmonella enterica clonal strains which, presenting a wide distribution potential, express resistance determinants that compromise effectiveness of the antimicrobial therapy. The screening was performed on 506 Salmonella enterica isolates from animals and humans, which were characterized by serovar and phage typing, genome macrorestriction and pulsed-field gel electrophoresis, and detection of phenotypic and genotypic traits for antimicrobial resistance. A Salmonella Enteritidis strain with strong quinolone resistance is spread on three host environments carrying one of the four variants found for the GyrA protein: (1) Asp87Tyr, the major polymorphism found in 39 Salmonella isolates from human origin and six from poultry; (2) Ser83Phe, with four isolates from human origin and one from white stork (Ciconia ciconia); and (3) Asp87Asn or (4) Asp87Gly, with two isolates each from human origins. Several Salmonella Typhimurium strains that presented int1 elements and the classically associated pentaresistance (ACSSuT) phenotype were found distributed between two host environments: domestic animals and humans, domestics and wild animals, or wild fauna plus humans. This study points out the importance of monitoring gut microbiota and its antimicrobial resistance from wildlife, in parallel to livestock animals and humans, especially for animal species that are in close contact with people.
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
Gonzalo
G
Palomo
Maria Jorge
MJ
Campos
María
M
Ugarte
María Concepción
MC
Porrero
Juan Manuel
JM
Alonso
Carmen
C
Borge
Santiago
S
Vadillo
Lucas
L
Domínguez
Alberto
A
Quesada
Segundo
S
Píriz
D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001434:Bacteriophage Typing; D027081:DNA Gyrase / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D011110:Polymorphism, Genetic; D011200:Poultry / Q000382:microbiology; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D027081:DNA Gyrase