Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice.
Heat shock proteins play an important role in plant stress tolerance and are mainly regulated by heat shock transcription factors (Hsfs). In this study, we generated transgenic rice over-expressing OsHsfA7 and carried out morphological observation and stress tolerance assays. Transgenic plants exhibited less, shorter lateral roots and root hair. Under salt treatment, over-expressing OsHsfA7 rice showed alleviative appearance of damage symptoms and higher survival rate, leaf electrical conductivity and malondialdehyde content of transgenic plants were lower than those of wild type plants. Meanwhile, transgenic rice seedlings restored normal growth but wild type plants could not be rescued after drought and re-watering treatment. These findings indicate that over-expression of OsHsfA7 gene can increase tolerance to salt and drought stresses in rice seedlings.
Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China.
Ai-Ling
AL
Liu
Jie
J
Zou
Cui-Fang
CF
Liu
Xiao-Yun
XY
Zhou
Xian-Wen
XW
Zhang
Guang-Yu
GY
Luo
Xin-Bo
XB
Chen
D019295:Computational Biology; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
D010940:Plant Proteins; D012965:Sodium Chloride