DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells.
DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells.  DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.
División de Genética, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, IMSS, Monterrey, México. elvacortes@cibinmty.net
E I
EI
Cortés-Gutiérrez
M I
MI
Dávila-Rodríguez
J L
JL
Fernández
C
C
López-Fernández
J
J
Gosálvez
D002610:Cheek / Q000473:pathology; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005821:Genetic Techniques; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017404:In Situ Hybridization, Fluorescence; D016877:Oxidants / Q000494:pharmacology
D016877:Oxidants; D006861:Hydrogen Peroxide