Effects of sodium hydroxide, sodium hypochlorite, and gaseous hydrogen peroxide on the natural properties of cancellous bone.
Processed xenegeneic cancellous bone represents an alternative to bone autograft. In order to observe the effects of present prion inactivation treatments on the natural properties of xenogeneic cancellous bones, we treated bovine bone granules with sodium hydroxide (NaOH), sodium hypochlorite (NaOCl), and gaseous hydrogen peroxide (gH2 O2 ) respectively in this study. The microstructure, composition, and mineral content of the granules were evaluated by scanning electron micrograph, energy dispersive X-ray spectroscopy, ash analysis, and micro-computed tomography. The biomechanical property was analyzed by a materials testing machine. The cytocompatibility was evaluated by using a mouse fibroblast cell line (3T3). The microstructure, organic content, and mechanical strength were dramatically altered at the surface of bone in both NaOH- and NaOCl-treated groups, but not in the gH2 O2 -treated group. Compared with the gH2 O2 -treated group, attachment and proliferation of 3T3 were reduced in either NaOH- or NaOCl-treated groups. As the consequence, gH2 O2 treatment may be a useful approach of disinfection for the preparation of natural cancellous bone with well-preserved structural, mechanical, and biological properties.
Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
Long
L
Bi
De-Cheng
DC
Li
Zhao-Song
ZS
Huang
Zhi
Z
Yuan
D016475:3T3 Cells; D000818:Animals; D001696:Biomechanical Phenomena; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology*; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005347:Fibroblasts / Q000187:drug effects; D005740:Gases; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D012972:Sodium Hydroxide / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D013314:Stress, Mechanical; D055114:X-Ray Microtomography
D004202:Disinfectants; D005740:Gases; D012972:Sodium Hydroxide; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite
Cancellous bone; Gaseous hydrogen peroxide; Prion; Sodium hydroxide; Sodium hypochlorite