Associations between NOS1AP single nucleotide polymorphisms (SNPs) and QT interval duration in four racial/ethnic groups in the Multi-Ethnic Study of Atherosclerosis (MESA).
BACKGROUND
QT is a risk factor for sudden cardiac death (SCD). A genome-wide association study identified NOS1AP variants associated with QT, which have been replicated in predominantly Caucasian (CAU) populations. We used the Multi-Ethnic Study of Atherosclerosis to examine association of QT with NOS1AP variants in an ethnically diverse cohort.


METHODS
Twenty-eight tagging SNPs spanning NOS1AP were genotyped in 2847 MESA participants (approximately equal numbers of CAU, African Americans (AFA), Hispanics (HIS), and Chinese (CHN)), age 45-84 years, without cardiovascular disease. QT was measured using 12-lead ECG. Associations between QT and NOS1AP variants were evaluated using linear regression, adjusted for heart rate, age, gender, and field center stratified by ancestry, using an additive inheritance model. Ancestry informative markers (AIMs) and principal components using AIMs were used as additional covariates.


RESULTS
More NOS1AP SNPs were associated with QT in CAU than the other races. In CAU, each copy of rs1932933 risk allele was associated with an increase in QT (4.9 msec, P = 7.20 × 10-7). Significant associations in CAU and HIS were located at the 5' end, while associations in CHN were located at the 3' end.


CONCLUSIONS
NOS1AP variants were associated with QT in CAU, with weaker evidence for selected variants in HIS and CHN. Location of significant SNPs varied across ancestry. We identified possible novel associations at the 3' end of NOS1AP, where we observed significant association with QT in CHN only. Genotyping within these regions may determine functional variants affecting QT and SCD risk. In addition, investigations are needed across ethnically diverse population cohorts.
Section on Cardiology, Wake Forest School of Medicine, Winston-Salem, NC, USA. sidharth.a.shah@gmail.com
Sidharth A
SA
Shah
David M
DM
Herrington
Timothy D
TD
Howard
Jasmin
J
Divers
Donna K
DK
Arnett
Greg L
GL
Burke
Weng Hong
WH
Kao
Xiuqing
X
Guo
David S
DS
Siscovick
Aravinda
A
Chakravarti
Joao A
JA
Lima
Bruce M
BM
Psaty
Gordon F
GF
Tomaselli
Stephen S
SS
Rich
Donald W
DW
Bowden
Wendy
W
Post
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D001741:Black or African American / Q000235:genetics; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001213:Asian / Q000235:genetics; D050197:Atherosclerosis / Q000208:ethnology* / Q000235:genetics*; D004562:Electrocardiography; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D055106:Genome-Wide Association Study; D005838:Genotype; D006630:Hispanic or Latino / Q000235:genetics; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D044465:White People / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C488081:NOS1AP protein, human