[The influence of the penetrating cryoprotectors on protein synthesis in the cell-free system of the rat liver].
The influence of the penetrating cryoprotector and Mg2+ ions on the protein-synthesizing activity of postmitochondrial supernantant of the rat liver as well as on aminoacylation processes has been investigated. The addition of the penetrating cryoprotectors--ethylene glycol and DMSO--resulted in the concentration-dependant reversible inhibition of the protein biosynthesis and aminoacylation reaction in the cell-free system. These cryoprotectors at low concentrations intensified the stimulating effect of Mg2+ on the cumulative protein synthesis in the cell-free system.
A K
AK
Gulevskiĭ
A Iu
AIu
Nikol'chenko
D000596:Amino Acids / Q000378:metabolism; D049148:Aminoacylation / Q000187:drug effects; D000818:Animals; D002474:Cell-Free System; D002498:Centrifugation; D002850:Chromatography, Gel; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019855:Ethylene Glycol / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012343:RNA, Transfer / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats
D000596:Amino Acids; D012343:RNA, Transfer; D019855:Ethylene Glycol; D008274:Magnesium; D004121:Dimethyl Sulfoxide