Preparation and characterization of anti-algal sustained-release granules and their inhibitory effects on algae.
The objectives of this work were to prepare and characterize an anti-algal sustained-release granule, then study its mode of action on Microcystis aeruginosa. The anti-algal sustained-release granule was prepared with artemisinin using alginate-chitosan microcapsule technology and characterized by a high performance liquid chromatography with an evaporative light-scattering detector, Fourier transform infrared spectral analysis, and a scanning electron microscope. The optimum preparation (in %, w/v) using the orthogonal method was: 2.5 sodium alginate; 0.25 chloride; 0.6 artemisinin; 2 calcium chloride; and 1.5 mL of the cross-linking agent, glutaraldehyde. These artemisinin sustained-release granules had a high encapsulation efficiency (up to 68%) and good release properties (release time of more than 40 d). Artemisinin sustained-release granules released cumulatively in a solution containing M. aeruginosa, and the stress on algae increased gradually within 30 d. Artemisinin sustained-release granules decreased the content of the soluble protein, Chlorophyll a in 30 d, increased the superoxide dismutase activity of M. aeruginosa, but exerted no effect on the soluble sugar content. Compared to direct dosing of artemisinin, algae can be inhibited longer and more effectively by the artemisinin sustained-release granules. The results of our research can aid in the development of new anti-algal sustained-release granules and lead to further study of their application in the field.
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, China. nilixiao@gmail.com
Lixiao
L
Ni
Kumud
K
Acharya
Gaoxiang
G
Ren
Shiyin
S
Li
Yiping
Y
Li
Yong
Y
Li
D000464:Alginates / Q000737:chemistry; D037621:Artemisinins / Q000737:chemistry / Q000633:toxicity; D002122:Calcium Chloride / Q000737:chemistry; D003692:Delayed-Action Preparations; D004202:Disinfectants / Q000633:toxicity*; D004339:Drug Compounding / Q000379:methods*; D005068:Eutrophication / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D050296:Microbial Viability; D046931:Microcystis / Q000187:drug effects*; D008863:Microspheres; D014871:Water Microbiology
D000464:Alginates; D037621:Artemisinins; D003692:Delayed-Action Preparations; D004202:Disinfectants; D006603:Hexuronic Acids; D020723:Glucuronic Acid; C031327:artemisinin; D002122:Calcium Chloride