Effect of fermentation inhibitors in the presence and absence of activated charcoal on the growth of Saccharomyces cerevisiae.
The acidic hydrolysis of biomass generates numerous inhibitors of fermentation, which adversely affect cell growth and metabolism. The goal of the present study was to determine the effects of fermentation inhibitors on growth and glucose consumption by Saccharomyces cerevisiae. We also conducted in situ adsorption during cell cultivation in synthetic broth containing fermentation inhibitors. In order to evaluate the effect of in situ adsorption on cell growth, five inhibitors, namely 5-hydroxymethylfurfural, levulinic acid, furfural, formic acid, and acetic acid, were introduced into synthetic broth. The existence of fermentation inhibitors during cell culture adversely affects cell growth and sugar consumption. Furfural, formic acid, and acetic acid were the most potent inhibitors in our culture system. The in situ adsorption of inhibitors by the addition of activated charcoal to the synthetic broth increased cell growth and sugar consumption. Our results indicate that detoxification of fermentation media by in situ adsorption may be useful for enhancing biofuel production.
Department of Biotechnology, Pukyong National University, Busan, 608-737, South Korea.
Sung-Koo
SK
Kim
Don-Hee
DH
Park
Se Hee
SH
Song
Young-Jung
YJ
Wee
Gwi-Taek
GT
Jeong
D000327:Adsorption; D000931:Antidotes / Q000737:chemistry / Q000493:pharmacokinetics*; D056804:Biofuels; D019149:Bioreactors; D002606:Charcoal / Q000737:chemistry / Q000493:pharmacokinetics*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000254:growth & development*
D000931:Antidotes; D056804:Biofuels; D003470:Culture Media; D002606:Charcoal