Efficient genome editing in zebrafish using a CRISPR-Cas system.
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA.
Woong Y
WY
Hwang
Yanfang
Y
Fu
Deepak
D
Reyon
Morgan L
ML
Maeder
Shengdar Q
SQ
Tsai
Jeffry D
JD
Sander
Randall T
RT
Peterson
J-R Joanna
JR
Yeh
J Keith
JK
Joung
D000818:Animals; D001483:Base Sequence; D064113:CRISPR-Cas Systems; D004247:DNA / Q000235:genetics; D053837:DNA Cleavage; D004625:Embryo, Nonmammalian; D004720:Endonucleases / Q000235:genetics; D005818:Genetic Engineering; D016678:Genome; D055029:Inverted Repeat Sequences; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D017393:RNA Editing; D015027:Zebrafish / Q000235:genetics*; D058727:RNA, Small Untranslated
D004247:DNA; D004720:Endonucleases; D058727:RNA, Small Untranslated