Evaluation and comparison of efficacy of three different storage media, coconut water, propolis, and oral rehydration solution, in maintaining the viability of periodontal ligament cells.
BACKGROUND
Two of the most critical factors affecting the prognosis of an avulsed tooth after replantation are extra oral dry time and the storage medium in which the tooth is placed before treatment is rendered. However, the ability of a storage/transport medium to support cell viability can be more important than the extra oral time to prevent ankylosis and replacement resorption.


OBJECTIVE
Purpose of this study was evaluation and comparison of efficacy of a new storage medium, oral rehydration solution (ORS) with coconut water, and propolis in maintaining the viability of periodontal ligament (PDL) cells by using a collagenase-dispase assay.


METHODS
40 teeth were selected with intact crown which were advised for Orthodontic extraction having healthy PDL. Teeth were then randomly divided into three experimental storage solution groups. Other 10 were divided into positive and negative control groups (5 each).  STATISTICAL ANALYSIS AND RESULT: The results were statistically analyzed with analysis of variance and multiple range by using post hoc tests. The results of the prevailing study indicated that coconut water group demonstrated a significantly higher number of viable PDL cells than propolis 50%, and ORS. There was no significant difference between coconut water and propolis 50% groups.
Department of Conservative Dentistry, K.M. Shah Dental College and Hospital, Sumandeep Vidhyapeeth, Waghodiya, Piparia, Gujarat, India.
Tulsi
T
Sanghavi
Nimisha
N
Shah
Vaishali
V
Parekh
Kiran
K
Singbal
Coconut water; oral rehydration solution; propolis