[2'-Modified oligoribonucleotides, containing 1,2-diol and aldehyde groups. Synthesis and properties].
1,2-Diol-oligoribonucleotides were prepared using fully protected 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxoethyl]uridine 3'-phosphoramidite. Incorporation of the 2'-modified uridine residue into oligonucleotide chains does not significantly affect the thermal stability of RNA and RNA-DNA duplexes. Periodate oxidation of the 1,2-diol results in reactive 2'-aldehyde oligoribonucleotides. Further application of these oligonucleotides for cross-linking with bacterial ribonuclease P was investigated.
E A
EA
Khomiakova
E M
EM
Zubin
L V
LV
Pavlova
E V
EV
Kazanova
I P
IP
Smirnov
G E
GE
Pozmogova
S
S
Muller
N G
NG
Dolinnaia
E A
EA
Kubareva
R K
RK
Harmann
T S
TS
Oretskaia
D000447:Aldehydes / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D043262:Ribonuclease P / Q000737:chemistry
D000447:Aldehydes; D001426:Bacterial Proteins; D009692:Nucleic Acid Heteroduplexes; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA; D043262:Ribonuclease P