TNF gene polymorphisms in cystic fibrosis patients: contribution to the disease progression.
BACKGROUND
It is well known that the disease progression in cystic fibrosis (CF) patients may be diverse in subjects with identical mutation in CFTR gene. It is quite possible that such heterogeneity is associated with TNF-α and/or LT-α gene polymorphisms since their products play a key role in inflammation. The aim of the study was to investigate the possible roles of TNF gene polymorphisms in CF disease phenotype and progression.


METHODS
198 CF patients and 130 control subjects were genotyped for both TNF-α-308GA and LT-α + 252AG polymorphisms.


RESULTS
The carriers of the TNF-α-308A allele more frequently had asthma as compared to patients homozygous for the TNF-α-308 G allele. In 9 of 108 (8.3%) of LTα + 252AA carriers, tuberculosis infection has been documented, whereas there was no case of tuberculosis among patients, either homozygous or heterozygous for LTα +252 G alleles (p = 0.01). We never observed virus hepatitis among LTα + 252GA carriers. The genotypes TNF-α-308GG - LT-α + 252AA and TNF-α-308GA - LT-α + 252AG were unfavorable with regard to liver disease development (both p < 0.05). It was also shown that neutrophil elastase activity was higher in sputum specimens from high TNF producers with genotypes TNF-α-308GA or LT-α + 252GG. In addition the carriers of such genotypes demonstrated a higher risk of osteoporosis development (p values were 0.011 and 0.017, respectively).


CONCLUSIONS
The carriers of genotypes, which are associated with higher TNF-α production, demonstrated increased frequency of asthma, higher levels of neutrophil elastase, and decrease of bone density. On the contrary, the carriers of genotypes associated with low TNF-α production showed a higher frequency of tuberculosis infection.
Department of Cystic Fibrosis, Research Centre for Medical Genetics, 1 Moskvorechie Street, Moscow 115478, Russia.
Galina
G
Shmarina
Alexander
A
Pukhalsky
Nika
N
Petrova
Ekaterina
E
Zakharova
Lucine
L
Avakian
Nikolai
N
Kapranov
Vladimir
V
Alioshkin
D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D002648:Child; D003550:Cystic Fibrosis / Q000235:genetics* / Q000503:physiopathology; D017931:DNA Primers; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D010024:Osteoporosis / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D017931:DNA Primers; D014409:Tumor Necrosis Factor-alpha