Actinobaculum suis detection using polymerase chain reaction.
Actinobaculum suis is an important agent related to urinary infection in swine females. Due to its fastidious growth characteristics, the isolation of this anaerobic bacterium is difficult, thus impairing the estimation of its prevalence. The purpose of this study was to develop and test a polymerase chain reaction (PCR) for the detection and identification of A. suis and then compare these results with traditional isolation methods. Bacterial isolation and PCR were performed on one hundred and ninety-two urine samples from sows and forty-five preputial swabs from boars. The results indicate that this PCR was specific for A. suis, presenting a detection limit between 1.0 × 10(1) CFU/mL and 1.0 × 10(2) CFU/mL. A. suis frequencies, as measured by PCR, were 8.9% (17/192) in sow urine samples and 82.2% (37/45) in preputial swabs. Assessed using conventional culturing techniques, none of the urine samples were positive for A. suis; however, A. suis was detected in 31.1% (14/45) of the swabs. This PCR technique was shown to be an efficient method for the detection of A. suis in urine and preputial swabs.
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Avenida Professor Dr. Orlando Marques de Paiva 87 Cidade Universitária, 05508 270 São Paulo, SP, Brazil.
Cristina Román
CR
Amigo
Debora Dirani
DD
Sena de Gobbi
Vasco Túlio de Moura
VT
Gomes
Danilo do Prado
Ddo P
Perina
Pedro Henrique
PH
Nogueira de Lima Filsner
Barbara Letícia Pereira
BL
Costa
Maria Garcia
MG
Spindola
Thais Sebastiana Porfida
TS
Ferreira
Paulo Eduardo
PE
Brandão
Andrea Micke
AM
Moreno
D000191:Actinomycetaceae / Q000235:genetics* / Q000302:isolation & purification; D000193:Actinomycetales Infections / Q000382:microbiology / Q000652:urine / Q000662:veterinary*; D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology* / Q000652:urine
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S