Protective effect of remote ischemic per-conditioning in the ischemia and reperfusion-induce renal injury in rats.
OBJECTIVE
To evaluate the protective effect of remote ischemic per-conditioning in ischemia and reperfusion-induced renal injury.


METHODS
Fifteen rats (Rattus norvegicus) were randomized into three groups (n = 5): Group Normality (GN), Control Ischemia and Reperfusion (GIR) and Group remote ischemic per-conditioning (GPER). With the exception of the GN group, all others underwent renal ischemia for 30 minutes. In group GPER we performed the ischemic remote per-conditioning, consisting of three cycles of ischemia and reperfusion applied every five minutes during the ischemic period, to the left hindlimb of the rats by means of a tourniquet. To quantify the lesions we measured serum levels of creatinine and urea, as well as analyzed renal histopathology.


RESULTS
The GPER group presented with better levels of urea (83.74 ± 14.58%) and creatinine (0.72 ± 26.14%) when compared to GIR group, approaching the GN group. Histopathologically, the lower levels of medullary congestion and hydropic degeneration were found in group GPER.


CONCLUSIONS
The remote ischemic per-conditioning had a significant protective effect on renal ischemia and reperfusion.
Laboratory of Experimental Surgery, State University of Pará – Pará, Brazil.
Vitor Nagai
VN
Yamaki
Thiago Barbosa
TB
Gonçalves
João Vitor Baia
JV
Coelho
Ruy Victor Simões
RV
Pontes
Felipe Lobato da Silva
FL
Costa
Marcus Vinicius Henriques
MV
Brito
D000818:Animals; D019194:Ischemic Preconditioning; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*