Age-associated changes in monocyte and innate immune activation markers occur more rapidly in HIV infected women.
BACKGROUND
Aging is associated with immune dysfunction and the related development of conditions with an inflammatory pathogenesis. Some of these immune changes are also observed in HIV infection, but the interaction between immune changes with aging and HIV infection are unknown. Whilst sex differences in innate immunity are recognized, little research into innate immune aging has been performed on women.


METHODS
This cross-sectional study of HIV positive and negative women used whole blood flow cytometric analysis to characterize monocyte and CD8(+) T cell subsets. Plasma markers of innate immune activation were measured using standard ELISA-based assays.


RESULTS
HIV positive women exhibited elevated plasma levels of the innate immune activation markers CXCL10 (p<0.001), soluble CD163 (sCD163, p = 0.001), sCD14 (p = 0.022), neopterin (p = 0.029) and an increased proportion of CD16(+) monocytes (p = 0.009) compared to uninfected controls. Levels of the innate immune aging biomarkers sCD163 and the proportion of CD16(+) monocytes were equivalent to those observed in HIV negative women aged 14.5 and 10.6 years older, respectively. CXCL10 increased with age at an accelerated rate in HIV positive women (p = 0.002) suggesting a synergistic effect between HIV and aging on innate immune activation. Multivariable modeling indicated that age-related increases in innate immune biomarkers CXCL10 and sCD163 are independent of senescent changes in CD8(+) T lymphocytes.


CONCLUSIONS
Quantifying the impact of HIV on immune aging reveals that HIV infection in women confers the equivalent of a 10-14 year increase in the levels of innate immune aging markers. These changes may contribute to the increased risk of inflammatory age-related diseases in HIV positive women.
Centre for Virology, Burnet Institute, Melbourne, Australia.
Genevieve E
GE
Martin
Maelenn
M
Gouillou
Anna C
AC
Hearps
Thomas A
TA
Angelovich
Allen C
AC
Cheng
Fiona
F
Lynch
Wan-Jung
WJ
Cheng
Geza
G
Paukovics
Clovis S
CS
Palmer
Richard M
RM
Novak
Anthony
A
Jaworowski
Alan L
AL
Landay
Suzanne M
SM
Crowe
D000328:Adult; D000367:Age Factors; D000375:Aging / Q000276:immunology*; D015703:Antigens, CD / Q000097:blood; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood; D015415:Biomarkers / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D058851:GPI-Linked Proteins / Q000276:immunology; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology; D006801:Humans; D007113:Immunity, Innate; D016130:Immunophenotyping; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000473:pathology; D019798:Neopterin / Q000097:blood; D011956:Receptors, Cell Surface / Q000097:blood; D017452:Receptors, IgG / Q000276:immunology; D000098902:CD163 Antigen
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D000098902:CD163 Antigen; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface; D017452:Receptors, IgG; D019798:Neopterin