Neurexin regulates visual function via mediating retinoid transport to promote rhodopsin maturation.
Neurexins are cell adhesion molecules involved in synapse formation and synaptic regulation. Mutations in the neurexin genes are linked to a number of neurodevelopmental disorders such as autism. Here, we show that the Drosophila homolog of α-Neurexin is critical for fly visual function. Lack of Neurexin leads to significantly impaired visual function due to reduced rhodopsin levels. We show that the decreased chromophore levels cause deficits in rhodopsin maturation and that Neurexin is required for retinoid transport. Using yeast two-hybrid screening, we identify that Neurexin interacts with apolipoprotein I (ApoL I), a product generated by cleavage of retinoid- and fatty acid-binding glycoprotein (RFABG) that functions in retinoid transport. Finally, we demonstrate that Neurexin is essential for the apolipoproteins level. Our results reveal a role for Neurexin in mediating retinoid transport and subsequent rhodopsin maturation and suggest that Neurexin regulates lipoprotein function.
Institute of Life Sciences, the Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China.
Yao
Y
Tian
Tao
T
Li
Mingkuan
M
Sun
Didi
D
Wan
Qian
Q
Li
Peipei
P
Li
Zi Chao
ZC
Zhang
Junhai
J
Han
Wei
W
Xie
D000818:Animals; D030801:Animals, Genetically Modified; D015816:Cell Adhesion Molecules, Neuronal / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D004331:Drosophila melanogaster; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D010775:Photic Stimulation / Q000379:methods; D021381:Protein Transport / Q000502:physiology; D012176:Retinoids / Q000235:genetics / Q000378:metabolism*; D012243:Rhodopsin / Q000502:physiology*; D014796:Visual Perception / Q000502:physiology*
D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D050556:Fatty Acid-Binding Proteins; D006023:Glycoproteins; C491321:Nrx protein, Drosophila; D012176:Retinoids; C101137:apolpp protein, Drosophila; D012243:Rhodopsin