Response of osteoblast-like cells to zirconia with different surface topography.
The objective of this study was to clarify the effect of surface topography on osteoblast-like cell behavior on yttria stabilized tetragonal zirconia polycrystals (TZP). Mirror-polished; blasted with 50- or 150-µm alumina (SB50 and SB150); and SB150 acid-etched with hydrofluoric acid (SB150E) were prepared on TZP. Initial attachment, proliferation and differentiation of MC3T3-E1 were evaluated. The scanning electron microscopy and Sdr (developed interfacial area ratio) values indicated that both micro- and nano-topographies produced on the SB150E surfaces. Although no clear differences were observed in initial cell attachment among specimens, the proliferation rate and expression of ALP activity on the SB150E specimens was significantly higher than that on the other specimens. These results indicate that the creation of micro- and nano-topographies on TZP by surface treatment offers a promising method of enhancing the proliferation and differentiation of MC3T3-E1 cells.
Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Mihama-ku, Chiba, Japan.
Hiroshi
H
Ito
Hodaka
H
Sasaki
Kensuke
K
Saito
Shinya
S
Honma
Yasutomo
Y
Yajima
Masao
M
Yoshinari
D016475:3T3 Cells; D000134:Acid Etching, Dental / Q000379:methods*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000537:Aluminum Oxide; D000704:Analysis of Variance; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006858:Hydrofluoric Acid; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties; D015019:Yttrium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
D024042:Collagen Type I; C499362:yttria stabilized tetragonal zirconia; D015675:Osteocalcin; D015019:Yttrium; D015040:Zirconium; D000469:Alkaline Phosphatase; D000537:Aluminum Oxide; D006858:Hydrofluoric Acid