Recombination and synaptic adjustment in oocytes of mice heterozygous for a large paracentric inversion.
Homologous chromosome synapsis in inversion heterozygotes results in the formation of inversion loops. These loops might be transformed into straight, non-homologously paired bivalents via synaptic adjustment. Synaptic adjustment was discovered 30 years ago; however, its relationship with recombination has remained unclear. We analysed this relationship in female mouse embryos heterozygous for large paracentric inversion In(1)1Rk using immunolocalisation of the synaptonemal complex (SYCP3) and mature recombination nodules (MLH1) proteins. The frequency of cells containing bivalents with inversion loops decreased from 69 % to 28 % during pachytene. If an MLH1 focus was present in the non-homologously paired inverted region of the straight bivalent, it was always located in the middle of the inversion. Most of the small, incompletely adjusted loops contained MLH1 foci near the points at which pairing partners were switched. This observation indicates that the degree of synaptic adjustment depended on the crossover position. Complete synaptic adjustment was only possible if a crossover (CO) was located exactly in the middle of the inversion. If a CO was located at any other site, this interrupted synaptic adjustment and resulted in inversion loops of different sizes with an MLH1 focus at or near the edge of the remaining loop.
Siberian Department, Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Anna A
AA
Torgasheva
Nikolai B
NB
Rubtsov
Pavel M
PM
Borodin
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins; D007446:Chromosome Inversion / Q000235:genetics*; D023902:Chromosome Pairing; D003434:Crossing Over, Genetic; D004268:DNA-Binding Proteins; D005260:Female; D006579:Heterozygote; D008540:Meiosis / Q000235:genetics; D051379:Mice; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development; D011995:Recombination, Genetic; D013573:Synaptonemal Complex / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C501485:Mlh1 protein, mouse; D009687:Nuclear Proteins; C490062:Sycp3 protein, mouse; D000070957:MutL Protein Homolog 1