Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules.
A novel polyelectrolyte nanocapsule system composed of biopolymers, chitosan and heparin has been fabricated by the layer-by-layer technique on silica nanoparticles followed by dissolution of the silica core. The nanocapsules were of the size range 200 ± 20 nm and loaded with the positively charged anticancer drug doxorubicin with an efficiency of 89%. The loading of the drug into the capsule happens by virtue of the pH-responsive property of the capsule wall, which is determined by the pKa of the polyelectrolytes. As the pH is varied, about 64% of the drug is released in acidic pH while 77% is released in neutral pH. The biocompatibility, efficiency of drug loading, and enhanced bioavailability of the capsule system was confirmed by MTT assay and in vivo biodistribution studies.
Department of Materials Engineering, Indian Institute of Science, Bangalore, India.
Midhun B
MB
Thomas
Krishna
K
Radhakrishnan
Divya P
DP
Gnanadhas
Dipshikha
D
Chakravortty
Ashok M
AM
Raichur
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism*; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry*; D055672:Static Electricity
D053769:Nanocapsules; D004317:Doxorubicin; D006493:Heparin; D048271:Chitosan
biocompatible; drug delivery; electrostatic interaction; layer-by-layer