The gene cluster aur1 for the angucycline antibiotic auricin is located on a large linear plasmid pSA3239 in Streptomyces aureofaciens CCM 3239.
We previously identified a polyketide synthase gene cluster, aur1, responsible for the production of the angucycline antibiotic auricin in Streptomyces aureofaciens CCM 3239. A sequence analysis of the aur1 flanking regions revealed the presence of several genes encoding proteins homologous to those for Streptomyces linear plasmid replication, partitioning and telomere-binding. Pulse-field gel electrophoresis detected the single, 240-kb linear plasmid, pSA3239, in S. aureofaciens CCM3239. The presence of the auricin cluster in pSA3239 was confirmed by several approaches. In addition to aur1, pSA3239 also carries a large number of regulatory genes, and two gene clusters involved in the production of secondary metabolites: the aur2 cluster for an unknown secondary metabolite and the bpsA cluster for the blue pigment indigoidine.
Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
Renata
R
Novakova
Renata
R
Knirschova
Marian
M
Farkasovsky
Lubomira
L
Feckova
Alena
A
Rehakova
Erik
E
Mingyar
Jan
J
Kormanec
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D018942:Macrolides / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids; D017422:Sequence Analysis, DNA; D013304:Streptomyces aureofaciens / Q000235:genetics* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; C505282:auricin