Selective fluorogenic chemosensors for distinct classes of nucleases.
NUCLEASE SENSOR TRIO: Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3'-exonucleases, 5'-exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media.
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 702-701, Korea.
Jong-Wha
JW
Jung
Sarah K
SK
Edwards
Eric T
ET
Kool
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004720:Endonucleases / Q000032:analysis* / Q000378:metabolism; D005092:Exonucleases / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism*; D013210:Staphylococcus / Q000201:enzymology
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011721:Pyrenes; D004247:DNA; C030984:pyrene; D004720:Endonucleases; D005092:Exonucleases