Single molecule tracking of P-glycoprotein in live cells reveals dynamic heterogeneity.
P-glycoprotein transports chemotherapy drugs from the plasma membrane and allows cancer cells to survive treatment. We transiently transfected PGP labeled with enhanced green fluorescent protein (PGP-EGFP) into MES-SA cells and used single molecule tracking techniques to characterize the dynamics on the surface of live cells. PGP exhibits freely diffusive behavior at short times and is confined at long times with a transition to anomalous diffusion at 0.7 s.
Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA. Philip.Cheney@du.edu
Philip P
PP
Cheney
Michael D
MD
Stachler
Michelle K
MK
Knowles
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins