Adherence of Aeromonas hydrophila strains to human enterocyte-like cells pre-infected with rotavirus.
BACKGROUND
The interest grown in these years about emerging pathogens in the onset of intestinal disease showed that the pathogenic mechanism is a multifactorial event. Our objective was to evaluate the role of co-infection with rotavirus in the expression of Aeromonas spp adhesiveness.


METHODS
The rate of co-infection involves contact of Caco-2 cells with the virus, followed by adsorption for 1 and 2 hours. Aliquots of bacterial suspensions were added to tissue-culture plates. After infection, cell monolayers were lysed; serially diluted lysates were plated to determine the number of bound bacteria by performing colony forming units (CFU) counts.


RESULTS
Non-adhesive strains were not subject to variations resulting from co-infection, while those who had medium or high adhesiveness gave rise to an increase of the same.


CONCLUSIONS
Infection with rotavirus promotes the Aeromonas ability to adhere to Caco-2 cells and this effect depends on the duration of infection and on the starting adhesiveness of bacteria strain.
Department of Hygiene, Preventive Medicine and Public Health, University of Messina, Italy. mp.bertuccio@gmail.com
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D016980:Aeromonas hydrophila / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology*; D018938:Caco-2 Cells / Q000382:microbiology*; D060085:Coinfection / Q000382:microbiology*; D020895:Enterocytes / Q000382:microbiology* / Q000821:virology*; D006801:Humans; D012401:Rotavirus / Q000472:pathogenicity* / Q000502:physiology