[Effect of ginsenoside Rg1 on functional expression of human neural stem cells: a patch clamp study].
OBJECTIVE
To observe the effects of ginsenoside Rg1 on the functional expression of human neural stem cells (hNSCs).


METHODS
The membrane electrophysiological properties and sodium and potassium ion channels in the hNSCs induced by Rg1 were analyzed using the whole-cell patch-clamp.


RESULTS
On the 7th day, the neuron-like cells derived from ginsenoside Rg1 (20 mg x L(-1))-induced NSCs show: (1) The resting membrane potential: (-45.70 +/- 2.63) mV, the membrane capacitance: (26.89 +/- 1.91) pF, the membrane input impedance: (877.51 +/- 20.44) MH (P < 0.05 compared with the control group, respectively); (2) The detection rate of inward sodium current which is rapidly activated and inactivated in voltage-dependence was 50%, and its average peak value was (711.48 +/- 158.03) pA (P < 0.05 compared with the control group); (3) The outward potassium currents were composed of rapidly activated and inactivated transient outward potassium current and delayed rectifier outward potassium current, and its average peak value was (1 070.42 +/- 177.18) pA (P < 0.05 compared with the control group).


CONCLUSIONS
Ginsenoside Rg1 can promote the functional expression and maturity of hNSCs.
Key Laboratory of Neurobiology of Chongqing, Research Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China.
Ying-Hong
YH
Jiang
Ying-Bo
YB
Li
Xiang-Qin
XQ
Zhao
Di
D
Chen
Rong
R
Jiang
Sha-Li
SL
Wang
D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D010936:Plant Extracts / Q000494:pharmacology*; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism
D036145:Ginsenosides; D010936:Plant Extracts; D015221:Potassium Channels; D015222:Sodium Channels; C035054:ginsenoside Rg1