miR-206 inhibits gastric cancer proliferation in part by repressing cyclinD2.
In this study, we detected miR-206 expression in gastric cancer (GC) and further investigated its effects on GC cell growth in vitro and in vivo. miR-206 expression was found to be significantly decreased in 30 GC samples and GC cell lines by real time-PCR. Restoration of miR-206 reduced cell growth and colony forming ability in GC cells with G0/G1 cell cycle arrest. Further studies demonstrated that miR-206 could suppress GC cells proliferation at least partially through targeting the cyclinD2 (CCND2). Therefore, we provided evidence that miR-206 was a potential tumor suppressor and may be used as a therapeutic target for gastric cancer.
State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Lin
L
Zhang
Xiaodong
X
Liu
Haifeng
H
Jin
Xuegang
X
Guo
Limin
L
Xia
Zhangqian
Z
Chen
Ming
M
Bai
Jian
J
Liu
Xin
X
Shang
Kaichun
K
Wu
Yanglin
Y
Pan
Daiming
D
Fan
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056742:Cyclin D2 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009841:Oligonucleotides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D016192:Resting Phase, Cell Cycle; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D047368:Tumor Burden
C080336:CCND2 protein, human; D056742:Cyclin D2; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides