Blue light induced free radicals from riboflavin on E. coli DNA damage.
The micronutrients in many cellular processes, riboflavin (vitamin B(2)), FMN, and FAD are photo-sensitive to UV and visible light to generate reactive oxygen species (ROS). The riboflavin photochemical treatment with UV light has been applied for the inactivation of microorganisms to serve as an effective and safe technology. Ultra-violet or high-intensity radiation is, however, considered as a highly risky practice. This study was working on the application of visible LED lights to riboflavin photochemical reactions to development an effective antimicrobial treatment. The photosensitization of bacterial genome with riboflavin was investigated in vitro and in vivo by light quality and irradiation dosage. The riboflavin photochemical treatment with blue LED light was proved to be able to inactivate E. coli by damaging nucleic acids with ROS generated. Riboflavin is capable of intercalating between the bases of bacterial DNA or RNA and absorbs lights in the visible regions. LED light illumination could be a more accessible and safe practice for riboflavin photochemical treatments to achieve hygienic requirements in vitro.
Department of Biotechnology, Ming-Chuan University, Gui-Shan 333, Taiwan.
Ji-Yuan
JY
Liang
Jeu-Ming P
JM
Yuann
Chien-Wei
CW
Cheng
Hong-Lin
HL
Jian
Chin-Chang
CC
Lin
Liang-Yu
LY
Chen
D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D004249:DNA Damage; D003855:Deoxyribose / Q000737:chemistry; D004867:Equipment Design; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D005609:Free Radicals; D008027:Light; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods; D017382:Reactive Oxygen Species / Q000737:chemistry; D012256:Riboflavin / Q000737:chemistry* / Q000494:pharmacology; D014466:Ultraviolet Rays
D000890:Anti-Infective Agents; D005609:Free Radicals; D017382:Reactive Oxygen Species; D003855:Deoxyribose; D012256:Riboflavin