Anti-inflammatory effects of a special carbohydrate-whey protein cake after exhaustive cycling in humans.
Intense exercise induces increased levels of pro-inflammatory and anti-inflammatory cytokines. Thus, the purpose of this study was to examine the effects of a special cake (consisting of carbohydrate to whey protein 3.5:1) vs. an isocaloric carbohydrate cake on inflammatory markers after exhaustive cycling in humans. Nine subjects received either the experimental or placebo cake in a counterbalanced fashion using a crossover, double-blind, repeated-measures design. They performed one trial involving a 2h exercise on a cycle ergometer at 60-65% VO2max followed by a 4h recovery and then a second trial involving an 1h exercise at 60-65% VO2max which was increased at 95% VO2max. Blood samples were collected pre-exercise, 30 min and 4h post-exercise, post-time Trial and 48 h post-time Trial. Cakes were consumed immediately post-exercise and every 1h for the next 3h. The results showed that consumption of the experimental cake reduced significantly (p<0.05), 4h post-exercise, the pro-inflammatory protein levels IL-6 and CRP compared to the control group by 50% and 46% respectively. Moreover, in the experimental cake group, the level of the anti-inflammatory cytokine IL-10 was higher by 118%, 4h post-exercise, compared to the control group but not statistically significant.
Department of Biochemistry & Biotechnology, University of Thessaly, Larisa 41221, Greece. Electronic address: e-f-thalia@hotmail.com.
Efthalia
E
Kerasioti
Dimitrios
D
Stagos
Athanasios
A
Jamurtas
Alexandra
A
Kiskini
Yiannis
Y
Koutedakis
Nikos
N
Goutzourelas
Spyros
S
Pournaras
Aristidis M
AM
Tsatsakis
Dimitrios
D
Kouretas
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001642:Bicycling; D002097:C-Reactive Protein / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D015444:Exercise; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008894:Milk Proteins / Q000494:pharmacology*; D000067816:Whey Proteins
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004040:Dietary Carbohydrates; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D008894:Milk Proteins; D000067816:Whey Proteins; D016753:Interleukin-10; D002097:C-Reactive Protein
ANOVA; BCAAs; C-reactive protein; CRP; CV; EDTA; ELISA; Exercise; IL; Inflammation; Interleukin-10; Interleukin-6; MDD; TBARS; TNF-a; Whey protein; analysis of variance; branched-chain amino acids; coefficients of variation; enzyme-linked immunosorbent assay; ethylenediamine tetraacetic acid; interleukin; minimum detectable dose; thiobarbituric acid reactive substances; tumour necrosis factor-a