Shear stress inhibits apoptosis of ischemic brain microvascular endothelial cells.
As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.
Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. drhys@sina.com.
Shan
S
Tian
Yulong
Y
Bai
Lin
L
Yang
Xinggang
X
Wang
Yi
Y
Wu
Jie
J
Jia
Yulian
Y
Zhu
Yong
Y
Cheng
Pengyue
P
Zhang
Junfa
J
Wu
Nianhong
N
Wang
Guang
G
Xia
Hua
H
Liao
Yuling
Y
Zhang
Xiafeng
X
Shen
Huixian
H
Yu
Yongshan
Y
Hu
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply*; D002545:Brain Ischemia; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D015870:Gene Expression; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D042787:Receptor, TIE-2 / Q000235:genetics / Q000378:metabolism; D012039:Regional Blood Flow; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033081:Shear Strength; D015398:Signal Transduction; D013314:Stress, Mechanical
D019253:Proto-Oncogene Proteins c-bcl-2; D042787:Receptor, TIE-2; D051057:Proto-Oncogene Proteins c-akt