Antimicrobial activity and phytochemical screening of Buchenavia tetraphylla (Aubl.) R. A. Howard (Combretaceae: Combretoideae).
This study evaluated the antimicrobial and hemolytic activities and phytochemical constituents of hydroalcoholic extract and its fractions from Buchenavia tetraphylla leaves. Cyclohexane (BTCF), ethyl acetate (BTEF), and n-butanol-soluble (BTSBF) and non-soluble (BTNBF) fractions were obtained from a liquid-liquid partition of hydroalcoholic extract (BTHE) from B. tetraphylla leaves. The hemolytic activity of active fractions was checked. The BTHE inhibited the growth of Micrococcus luteus (MIC: 0.10 mg/mL), Pseudomonas aeruginosa (MIC: 0.20 mg/mL), Mycobacterium smegmatis (MIC: 0.39 mg/mL), Proteus vulgaris, and Staphylococcus aureus (MIC: 0.78 mg/mL for both). The more active fractions were BTCF and BTBSF. BTCF showed better potential to inhibit M. luteus (0.10 mg/mL), P. aeruginosa (0.20 mg/mL), S. enteritidis (0.39 mg/mL), and S. aureus (1.56 mg/mL). BTBSF showed the best results for M. luteus (0.10 mg/mL), M. smegmatis, B. subtilis (0.39 mg/mL for both), and P. vulgaris (0.10 mg/mL). The HC50 were greater than observed MIC: 20.30, 4.70 and 2.53 mg/mL, respectively, to BTBF, BTHE and BTCF, which. The phytochemical analysis detected the presence of flavanoids, triterpene, carbohydrate, and tannin. Our work showed for the first time the broad-spread antimicrobial activity of B. tetraphylla, which has nonhemolytic action, creating a new perspective on the interesting association of traditional and scientific knowledge.
Laboratório de Produtos Naturais, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rêgo, s/n Cidade Universitária, 50670-420 Recife, PE, Brazil.
Ygor Lucena Cabral
YL
de Oliveira
Luís Cláudio
LC
Nascimento da Silva
Alexandre Gomes
AG
da Silva
Alexandre José
AJ
Macedo
Janete Magali
JM
de Araújo
Maria Tereza dos Santos
MT
Correia
Márcia Vanusa
MV
da Silva
D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis; D029764:Combretaceae / Q000737:chemistry*; D003510:Cyclohexanes / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000032:analysis; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects / Q000254:growth & development; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011514:Proteus vulgaris / Q000187:drug effects / Q000254:growth & development; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D012995:Solubility; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013634:Tannins / Q000032:analysis; D014315:Triterpenes / Q000032:analysis
D000085:Acetates; D000900:Anti-Bacterial Agents; D002241:Carbohydrates; D003510:Cyclohexanes; D005419:Flavonoids; D010936:Plant Extracts; D013634:Tannins; D014315:Triterpenes; C506365:Cyclohexane; C007650:ethyl acetate; D020001:1-Butanol