Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.
The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60° can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.
Departments of Chemistry and ‡Electrical Engineering, Stanford University , Stanford, California 94305, United States.
Matthew D
MD
Lew
Mikael P
MP
Backlund
W E
WE
Moerner
D004058:Diffusion; D006801:Humans; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D012399:Rotation