Increased levels of oxidative stress markers in the peritoneal fluid of women with endometriosis.
OBJECTIVE
To evaluate 8-hydroxy-2-deoxyguanosine (8-OHdG) and 8-isoprostane levels in the peritoneal fluid (PF) of women with endometriosis.


METHODS
One hundred and ten women with laparoscopically and histopathologically confirmed endometriosis and, as reference groups, 119 patients with simple serous (n=78) and dermoid (n=41) ovarian cysts were studied. Peritoneal fluid 8-OHdG and 8-isoprostane concentrations were evaluated by enzyme-linked immunosorbent assays.


RESULTS
8-OHdG and 8-isoprostane levels in peritoneal fluid were significantly higher in patients with endometriosis compared with the reference groups. Higher PF 8-OHdG and 8-isoprostane concentrations were observed in patients with advanced stages of endometriosis. A statistically significant positive correlation was found between 8-OHdG and 8-isoprostane levels in peritoneal fluid.


CONCLUSIONS
Endometriosis induces greater oxidative stress and frequent DNA mutations in peritoneal fluid than nonendometriotic ovarian cysts. The most severe oxidative stress occurs in the peritoneal cavity of women with more advanced stages of the disease.
1st Department of Oncological Gynecology and Gynecology, Medical University of Lublin, 20-081 Lublin, Staszica 16, Poland. polakg@yahoo.com
Grzegorz
G
Polak
Iwona
I
Wertel
Bartłomiej
B
Barczyński
Wojciech
W
Kwaśniewski
Wiesława
W
Bednarek
Jan
J
Kotarski
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D003560:Cysts / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D003884:Dermoid Cyst / Q000276:immunology / Q000378:metabolism; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D004715:Endometriosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D010048:Ovarian Cysts / Q000276:immunology / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D010051:Ovarian Neoplasms / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress; D010537:Peritoneum / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012720:Severity of Illness Index; D015854:Up-Regulation; D055815:Young Adult
D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine