Long wavelength fluorescence ratiometric zinc biosensor.
A protein-based emission ratiometric fluorescence biosensor is described that exhibits sensitivity to free zinc ion in solution down to picomolar concentrations. Ratiometric measurements are widely used to assure accurate quantitation, and emission ratios are preferred for laser scanning microscopes such as confocal fluorescence microscopes. The relatively long emission wavelengths used are well suited to studies in tissues and other matrices which exhibit significant fluorescence background, and the apo-carbonic anhydrase moiety recognizes zinc ion with high and controllable specificity.
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Hui Hui
HH
Zeng
Evgenia G
EG
Matveeva
Andrea K
AK
Stoddard
Carol A
CA
Fierke
Richard B
RB
Thompson
D001051:Apoenzymes / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D024402:Carbonic Anhydrase II / Q000737:chemistry / Q000378:metabolism; D020134:Catalytic Domain; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
C417664:Alexa594; D001051:Apoenzymes; D009930:Organic Chemicals; D024402:Carbonic Anhydrase II; D015032:Zinc