PEG-modified macroporous poly(glycidyl methacrylate) and poly(2-hydroxyethyl methacrylate) microspheres to reduce non-specific protein adsorption.
To minimize non-specific protein adsorption on macroporous poly(glycidyl methacrylate) and poly(2-hydroxyethyl methacrylate) microspheres containing amino and/or carboxyl groups, the microspheres are coated with α,ω-bis-carboxy poly(ethylene glycol) and amino-terminated poly(ethylene glycol-co-propylene glycol) or α-methoxy-ω-amino poly(ethylene glycol). Adsorption of bovine serum albumin (BSA), γ-globulin, (125) I-BSA, pepsin, and chymotrypsin on neat and PEGylated microspheres is determined by UV-VIS spectroscopy of supernatants and eluates or by measurement of radioactivity in an ionization chamber. Neat and PEGylated microspheres adsorb 0.8-70% and 0.02-44% of protein, respectively.
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic.
Helena
H
Hlídková
Daniel
D
Horák
Vladimír
V
Proks
Zdenka
Z
Kučerová
Michal
M
Pekárek
Jan
J
Kučka
D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D002918:Chymotrypsin / Q000737:chemistry; D007457:Iodine Radioisotopes / Q000737:chemistry; D008863:Microspheres; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D005719:gamma-Globulins / Q000737:chemistry
D007457:Iodine Radioisotopes; D011109:Polymethacrylic Acids; D005719:gamma-Globulins; C042535:polyglycidyl methacrylate; D011102:Polyhydroxyethyl Methacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D002918:Chymotrypsin