Expression patterns suggest that despite considerable functional redundancy, galectin-4 and -6 play distinct roles in normal and damaged mouse digestive tract.
The galectin-4 protein is mostly expressed in the digestive tract and is associated with lipid raft stabilization, protein apical trafficking, wound healing, and inflammation. While most mammalian species, including humans, have a single Lgals4 gene, some mice have two paralogues: Lgals4 and Lgals6. So far, their significant similarities have hindered the analysis of their respective expression and function. We took advantage of two antibodies that discriminate between the galectin-4 and galectin-6 proteins to document their patterns of expression in the normal and the dextran sodium sulfate (DSS)-damaged digestive tract in the mouse. In the normal digestive tract, their pattern of expression from tongue to colon is quite similar, which suggests functional redundancy. However, the presence of galectin-4, but not galectin-6, in the lamina propria of the DSS-damaged colon, its association with luminal colonic bacteria, and differences in subcellular localization of these proteins suggest that they also have distinct roles in the normal and the damaged mouse digestive tract. Our results provide a rare example of ancestral and derived functions evolving after tandem gene duplication.
Equipe Génétique et Evolution, UMR7138, CNRS and Université Pierre et Marie Curie, 75252 Paris Cedex 05, France. denis.houzelstein@snv.jussieu.fr
Denis
D
Houzelstein
Edouard
E
Reyes-Gomez
Marie
M
Maurer
Pierre
P
Netter
Dominique
D
Higuet
D000595:Amino Acid Sequence; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000009:adverse effects; D005260:Female; D037541:Galectin 4 / Q000737:chemistry / Q000378:metabolism*; D037161:Galectins / Q000737:chemistry / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D021381:Protein Transport
D037541:Galectin 4; D037161:Galectins; C110383:Lgals6 protein, mouse; D016264:Dextran Sulfate