Comparison of Botrytis cinerea populations isolated from two open-field cultivated host plants.
The necrotrophic fungus Botrytis cinerea is reported to infect more than 220 host plants worldwide. In phylogenetical-taxonomical terms, the pathogen is considered a complex of two cryptic species, group I and group II. We sampled populations of B. cinerea on sympatric strawberry and raspberry cultivars in the North-East of Hungary for three years during flowering and the harvest period. Four hundred and ninety group II B. cinerea isolates were analyzed for the current study. Three different data sets were generated: (i) PCR-RFLP patterns of the ADP-ATP translocase and nitrate reductase genes, (ii) MSB1 minisatellite sequence data, and (iii) the fragment sizes of five microsatellite loci. The structures of the different populations were similar as indicated by Nei's gene diversity and haplotype diversity. The F statistics (Fst, Gst), and the gene flow indicated ongoing differentiation within sympatric populations. The population genetic parameters were influenced by polymorphisms within the three data sets as assessed using Bayesian algorithms. Data Mining analysis pointed towards the five microsatellite loci as the most defining markers to study differentiation in the 490 isolates. The results suggest the occurrence of host-specific, sympatric divergence of generalist phytoparasites in perennial hosts.
Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary; Institute of Food Processing, Quality Assurance and Microbiology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi út 138, 4032 Debrecen, Hungary.
Mojtaba
M
Asadollahi
Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.
Erzsébet
E
Fekete
Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.
Levente
L
Karaffa
Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.
Michel
M
Flipphi
Sámuel Diószegi Institute of Agricultural Innovation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi út 138, 4032 Debrecen, Hungary.
Mariann
M
Árnyasi
Department of Computer Science, Islamic Azad University, Kashan Branch, Kashan, Iran.
Mahdi
M
Esmaeili
KRC Research Institute for Viticulture and Enology, Kőlyuktető, PO Box 83, 3301 Eger, Hungary.
Kálmán Zoltán
KZ
Váczy
Institute of Food Processing, Quality Assurance and Microbiology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi út 138, 4032 Debrecen, Hungary. Electronic address: karaffa@agr.unideb.hu.
Erzsébet
E
Sándor
D020171:Botrytis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics; D031985:Fragaria / Q000382:microbiology*; D014644:Genetic Variation; D058507:Host Specificity; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D027824:Rosaceae / Q000382:microbiology*
D004271:DNA, Fungal