Liposome-quantum dot complexes enable multiplexed detection of attomolar DNAs without target amplification.
Sensitive detection of DNA usually relies on target amplification approaches such as polymerase chain reaction and rolling circle amplification. Here we describe a new approach for sensitive detection of low-abundance DNA using liposome-quantum dot (QD) complexes and single-particle detection techniques. This assay allows for detection of single-stranded DNA at attomolar concentrations without the involvement of target amplification. Importantly, this strategy can be employed for simultaneous detection of multiple DNA targets.
Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong 518055, China.
Juan
J
Zhou
Qiang-xin
QX
Wang
Chun-yang
CY
Zhang
D004277:DNA, Single-Stranded / Q000032:analysis*; D008081:Liposomes / Q000737:chemistry*; D045663:Quantum Dots
D004277:DNA, Single-Stranded; D008081:Liposomes