Comparison of two biochemical methods for identifying Corynebacterium pseudotuberculosis isolated from sheep and goats.
The biochemical pattern of Cowan and Steel (BPCS) was compared with a commercial biochemical strip for the identification of Corynebacterium pseudotuberculosis isolated from small ruminants. On 16S rRNA gene sequencing, 40/78 coryneform isolates from the lymph nodes of sheep and goats with lesions resembling caseous lymphadenitis were identified as C. pseudotuberculosis. The sensitivities of the BPCS and the commercial biochemical strip relative to 16S rRNA sequencing were 80% and 85%, and their specificities were 92.1% and 94.7%, respectively; the level of agreement between the BPCS and the commercial biochemical strip was high (κ=0.82). Likelihood ratios for positive and negative results were 10.0 and 0.22 for the BPCS, and 16.0 and 0.16 for the commercial biochemical strip, respectively. These results indicate that the BPCS and the commercial biochemical strip are both useful for identifying C. pseudotuberculosis in veterinary microbiology laboratories.
Departamento de Sanidad Animal, Facultad de Veterinaria CEIA3, Campus Universitario de Rabanales, 14071 Córdoba, Spain.
Belén
B
Huerta
Lidia
L
Gómez-Gascón
Ana I
AI
Vela
José F
JF
Fernández-Garayzábal
Almudena
A
Casamayor
Carmen
C
Tarradas
Alfonso
A
Maldonado
D000818:Animals; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000302:isolation & purification*; D015511:Goat Diseases / Q000175:diagnosis / Q000382:microbiology*; D006041:Goats; D012329:RNA, Bacterial / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011934:Reagent Strips