Oral mucosal adhesive films containing royal jelly accelerate recovery from 5-fluorouracil-induced oral mucositis.
Oral mucositis induced by chemotherapy or radiotherapy has an impact upon quality-of-life, is dose-limiting for chemotherapy, and causes considerable morbidity. We evaluated the effect of royal jelly (RJ) on 5-fluorouracil (5-FU)-induced oral mucositis in hamsters. Oral mucositis was induced in hamsters through a combination of 5-FU treatment and mild abrasion of the cheek pouch. RJ was contained in chitosan-sodium alginate film (RJ film). Films were attached to the oral mucosa and the healing process examined by measuring the area of mucositis, myeloperoxidase (MPO) activity, microscopic aspects, and RT-PCR for detection of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β). Furthermore, we evaluated the radical-scavenging activity of RJ and generation of keratinocyte growth factor from human periodontal ligament fibroblasts. RJ films (10%, 30%) significantly improved recovery from 5-FU-induced damage, reduced MPO activity and the production of pro-inflammatory cytokines. Additionally, RJ showed radical-scavenging activity. These data suggest that topical application of films that contain RJ had a healing effect on the severe oral mucositis induced by 5-FU and that the effect was caused by the anti-inflammatory or anti-oxidative activities of RJ.
Division of Pharmacy, Ehime University Hospital, Ehime, Japan. kinnin@m.ehime-u.ac.jp
Shinichi
S
Watanabe
Katsuya
K
Suemaru
Kenshi
K
Takechi
Hiroaki
H
Kaji
Kimie
K
Imai
Hiroaki
H
Araki
D000269:Adhesives / Q000008:administration & dosage*; D000818:Animals; D002610:Cheek / Q000473:pathology; D006224:Cricetinae; D005227:Fatty Acids / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D051523:Fibroblast Growth Factor 7 / Q000378:metabolism; D005472:Fluorouracil / Q000009:adverse effects*; D016166:Free Radical Scavengers / Q000008:administration & dosage*; D053583:Interleukin-1beta / Q000378:metabolism; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D013280:Stomatitis / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000099219:Royal Jelly
D000269:Adhesives; D005227:Fatty Acids; D051523:Fibroblast Growth Factor 7; D005472:Fluorouracil; D016166:Free Radical Scavengers; D053583:Interleukin-1beta; D009195:Peroxidase; D014409:Tumor Necrosis Factor-alpha; D000099219:Royal Jelly