Helicase-mediated changes in RNA structure at the single-molecule level.
RNA helicases are a diverse group of RNA-dependent ATPases known to play a large number of biological roles inside the cell, such as RNA unwinding, remodeling, export and degradation. Understanding how helicases mediate changes in RNA structure is therefore of fundamental interest. The advent of single-molecule spectroscopic techniques has unveiled with unprecedented detail the interplay of RNA helicases with their substrates. In this review, we describe the characterization of helicase-RNA interactions by single-molecule approaches. State-of-the-art techniques are presented, followed by a discussion of recent advancements in this exciting field.
Institute of Inorganic Chemistry, University of Zurich, Zurich, Switzerland.
Sebastian L B
SL
König
Pramodha S
PS
Liyanage
Roland K O
RK
Sigel
David
D
Rueda
D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D059370:RNA Folding; D020365:RNA Helicases / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D012313:RNA; D020365:RNA Helicases
AFM; DEAD-box; FRET; PIFE; RNA folding; RNA helicase; optical tweezers; single-molecule spectroscopy