In vitro and in vivo enhancement of osteogenic capacity in a synthetic BMP-2 derived peptide-coated mineralized collagen composite.
Enhancement of osteogenic capacity was achieved in a mineralized collagen composite, nano-hydroxyapatite/collagen (nHAC), by loading with synthetic peptides derived from BMP-2 residues 32-48 (P17-BMP-2). Rabbit marrow stromal cells (MSCs) were used in vitro to study cell biocompatibility, attachment and differentiation on the mineralized collagen composite by a cell counting kit, scanning electron microscopy (SEM) and real-time reversed transcriptase-polymerase chain reaction analysis (RT-PCR). Optimal peptide dosage (1.0 µg/mL) was obtained by RT-PCR analysis in vitro. In addition, the relative expression level of OPN and OCN was significantly upregulated on P17-BMP-2/nHAC compared with nHAC. In vitro results of P17-BMP-2 release kinetics demonstrated that nHAC released P17-BMP-2 in a controlled and sustained manner. In the rabbit mandibular box-shaped bone defect model, osteogenic capacity of three groups (nHAC, P17-BMP-2/nHAC, rhBMP-2/nHAC) was evaluated. Compared to the nHAC group, bone repair responses in both P17-BMP-2/nHAC and rhBMP-2/nHAC group implants were significantly improved based on histological analysis. The osteogenic response of the P17-BMP-2/nHAC group was similar to that of the rhBMP-2/nHAC group.
Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, 110001, China.
Xue
X
Zhang
Beijing Allgens Medical Science & Technology Company, Beijing, 100085, China.
Wen-Guang
WG
Guo
Beijing Allgens Medical Science & Technology Company, Beijing, 100085, China.
Helen
H
Cui
Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, 110001, China.
Huan-Ye
HY
Liu
Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, 110001, China.
Yang
Y
Zhang
Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 6, D-55128, Mainz, Germany.
Werner E G
WE
Müller
Institute of Regenerative Medical Materials, Department of Materials Science & Engineering, Tsinghua University, Beijing, 100084, China.
Fu-Zhai
FZ
Cui
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D007700:Kinetics; D008903:Minerals / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D008903:Minerals; D010455:Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C509317:nano-hydroxyapatite-collagen; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen; D017886:Durapatite
P17-BMP-2; bone regeneration; bone tissue engineering; marrow stromal cells; nano-hydroxyapatite/collagen; osteoblastic differentiation