Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor.
F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through ε-subunit antibody-biotin-streptavidin-biotin-AC5-Sulfo-Osu system. Based on probe-DNA specific binding, DNA of several foodborne pathogens Listeria monocytogenes, Salmonella typhimurium, Vibrio parahaemolyticus and Vibrio cholerae was specifically captured by F0F1-ATPase molecular motor biosensors. Loads of DNA decreased the rotation rate of F0F1-ATPase, and led to the decrease of ATP synthesis. The detection of pathogens based on proton flux change driven by ATP-synthesis of F0F1-ATPase, which was indicated by F-DHPE, was monitored by a fluorescence spectrometer. The results demonstrate that the F0F1-ATPase molecular motor biosensor can specifically detect bacterial DNA at low concentration level, and will be a convenient, quick, and promising tool for detecting pathogens.
Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, China.
Jie
J
Zhang
Zhaojie
Z
Li
Huiyuan
H
Zhang
Jing
J
Wang
Yan
Y
Liu
Guangquan
G
Chen
D000255:Adenosine Triphosphate / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005452:Fluoresceins / Q000032:analysis; D005453:Fluorescence; D005516:Food Microbiology / Q000379:methods*; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000032:analysis; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification; D014733:Vibrio / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005452:Fluoresceins; C505938:N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt; D010714:Phosphatidylethanolamines; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases