Effects of acute systemic administration of cannabidiol on sleep-wake cycle in rats.
Cannabidiol (CBD) is one of the main components of Cannabis sativa and has a wide spectrum of action, including effects in the sleep-wake cycle.


OBJECTIVE
The objective of this paper is to assess the effects on sleep of acute systemic administration of CBD.


METHODS
Adult male Wistar rats were randomly distributed into four groups that received intraperitoneal injections of CBD 2.5 mg/kg, CBD 10 mg/kg, CBD 40 mg/kg or vehicle (n=seven animals/group). Sleep recordings were made during light and dark periods for four days: two days of baseline recording, one day of drug administration (test), and one day after drug (post-test).


RESULTS
During the light period of the test day, the total percentage of sleep significantly increased in the groups treated with 10 and 40 mg/kg of CBD compared to placebo. REM sleep latency increased in the group injected with CBD 40 mg/kg and was significantly decreased with the dose of 10 mg/kg on the post-test day. There was an increase in the time of SWS in the group treated with CBD 40 mg/kg, although this result did not reach statistical significance.


CONCLUSIONS
The systemic acute administration of CBD appears to increase total sleep time, in addition to increasing sleep latency in the light period of the day of administration.
Department of Neuroscience and Behavior of the Ribeirão Preto Medical School, University of São Paulo, INCT Translational Medicine (CNPq), São Paulo, Brazil. mchagas@fmrp.usp.br
Marcos Hortes N
MH
Chagas
José Alexandre S
JA
Crippa
Antonio Waldo
AW
Zuardi
Jaime E C
JE
Hallak
João Paulo
JP
Machado-de-Sousa
Camila
C
Hirotsu
Lucas
L
Maia
Sergio
S
Tufik
Monica Levy
ML
Andersen
D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology; D002185:Cannabidiol / Q000008:administration & dosage* / Q000009:adverse effects / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006993:Hypnotics and Sedatives / Q000008:administration & dosage* / Q000009:adverse effects / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012890:Sleep / Q000187:drug effects*; D012895:Sleep, REM / Q000187:drug effects; D014851:Wakefulness / Q000187:drug effects*
D000928:Antidepressive Agents; D006993:Hypnotics and Sedatives; D002185:Cannabidiol