Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.
A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.
Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Shota
S
Morimoto
Takenori
T
Tomohiro
Nobuyuki
N
Maruyama
Yasumaru
Y
Hatanaka
D001665:Binding Sites; D003374:Coumarins / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D019920:Photoaffinity Labels / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry; D033921:Vesicular Transport Proteins / Q000737:chemistry*
D003374:Coumarins; D008024:Ligands; D010455:Peptides; D019920:Photoaffinity Labels; D033921:Vesicular Transport Proteins; C030123:coumarin