[The ALV-A/B specific antibodies correlation between ELISA and IFA detection in chicken serum].
To study the correlation between ELISA and IFA tests in detection of ALV-A/B antibody in chicken sera, ELSA S/P values and IFA titers for different serum samples were measured and statistically analyzed. The results indicated that there was a strong positive correlation between ELISA S/P values and IFA titers (r = 0.97435, P < 0.001). Because the positive correlation between ELISA and IFA was so strong and antibody positive rates were identical in two tests, it suggested that IFA could be used as a alternative method to replace ELISA kit when only limited numbers of samples to be tested to reduce the cost and increase the sensitivity.
College of Animal Science and Technology, Shandong Agricultural University, Shandong Engineering Research Center for Animal Disease Control and Prevention, Taian 271018, China. lixue880209@163.com
Xue
X
Li
De-Qing
DQ
Li
Peng
P
Zhao
Zhi-Zhong
ZZ
Cui
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001353:Avian Leukosis / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D001354:Avian Leukosis Virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D002460:Cell Line; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D013045:Species Specificity
D000914:Antibodies, Viral