Uptake and processing of semiconductor quantum dots in living cells studied by fluorescence lifetime imaging microscopy (FLIM).
Carboxylate-terminated and dopamine-conjugated CdSe-ZnS quantum dots (QDs) are imaged in living fibroblasts using fluorescence lifetime imaging microscopy. Changes in lifetime are observed as the QDs are processed in the cells, and are consistent with lifetime measurements in bulk solution using buffers compositions that correspond to different cellular regions.
Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada.
Lina
L
Carlini
Jay L
JL
Nadeau
D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry; D004298:Dopamine / Q000737:chemistry; D008027:Light; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction; D045663:Quantum Dots; D012666:Semiconductors
D002264:Carboxylic Acids; D004298:Dopamine