Electrochemiluminescence biosensor based on conducting poly(5-formylindole) for sensitive detection of Ramos cells.
A signal-on electrochemiluminescence (ECL) biosensor devoted to the detection of Ramos cells was fabricated based on a novel conducting polymer, poly(5-formylindole) (P5FIn), which was synthesized electrochemically by direct anodic oxidation of 5-formylindole (5FIn). This ECL platform was presented by covalently coupling the 18-mer amino-substituted oligonucleotide (ODN) probes with aldehyde groups that are strongly reactive toward a variety of nucleophiles on the surface of solid substrates. The specific identification and high-affinity between aptamers and target cells, gold nanoparticles (AuNPs) enhanced ECL nanoprobes, along with P5FIn induced ECL quenching contributed greatly to the sensitivity and selectivity. The ECL signals were logarithmically linear with the concentration of Ramos cells in a wide determination range from 500 to 1.0 × 10(5) cells mL(-1), and the corresponding detection limit was 300 cells mL(-1).
State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China. gmnie@126.com
Guangming
G
Nie
Zhimin
Z
Bai
Wenying
W
Yu
Juan
J
Chen
D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D004566:Electrodes; D006046:Gold / Q000032:analysis / Q000737:chemistry; D006801:Humans; D007211:Indoles / Q000737:chemistry; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis
D052157:Aptamers, Nucleotide; D007211:Indoles; D006046:Gold