DMD-based LED-illumination super-resolution and optical sectioning microscopy.
Super-resolution three-dimensional (3D) optical microscopy has incomparable advantages over other high-resolution microscopic technologies, such as electron microscopy and atomic force microscopy, in the study of biological molecules, pathways and events in live cells and tissues. We present a novel approach of structured illumination microscopy (SIM) by using a digital micromirror device (DMD) for fringe projection and a low-coherence LED light for illumination. The lateral resolution of 90 nm and the optical sectioning depth of 120 μm were achieved. The maximum acquisition speed for 3D imaging in the optical sectioning mode was 1.6×10(7) pixels/second, which was mainly limited by the sensitivity and speed of the CCD camera. In contrast to other SIM techniques, the DMD-based LED-illumination SIM is cost-effective, ease of multi-wavelength switchable and speckle-noise-free. The 2D super-resolution and 3D optical sectioning modalities can be easily switched and applied to either fluorescent or non-fluorescent specimens.
State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
Dan
D
Dan
Ming
M
Lei
Baoli
B
Yao
Wen
W
Wang
Martin
M
Winterhalder
Andreas
A
Zumbusch
Yujiao
Y
Qi
Liang
L
Xia
Shaohui
S
Yan
Yanlong
Y
Yang
Peng
P
Gao
Tong
T
Ye
Wei
W
Zhao
D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D008027:Light; D008029:Lighting / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*