VKORC1 C1173T and VKORC1 G-1639A gene polymorphisms in Turkish Behçet's patients with ocular and non-ocular involvement.
BACKGROUND
Behçet's disease (BD) is a multisystemic vasculitis with unknown etiology. Vitamin K epoxide reductase complex subunit 1 (VKORC1) is the key enzyme in the formation of active vitamin K that is a cofactor of various coagulation factors. Polymorphisms of the VKORC1 may affect the levels of active forms of vitamin K-dependent coagulation proteins and the tendency to thrombosis. The current study aimed to evaluate the role of VKORC1 gene polymorphisms in ocular and non-ocular Behçet's disease.


METHODS
VKORC1 C1173T (rs 9934438) and G-1639A (rs 9923231) gene polymorphisms were evaluated by real-time polymerase chain reaction-based DNA analysis. The frequency of alleles and distribution of genotypes were assessed by the chi-squared test. Genotype distribution and Hardy-Weinberg equilibrium were tested with the χ(2) test for quality of fit.


RESULTS
The distribution of GG, GA and AA and CC, CT, TT genotypes and the frequency of G,A and C,T alleles were not found to be different between patients and controls (p = 0.5651; p = 0.335 respectively), as well as patients with or without eye involvement (p = 0.9267; p = 0.384 respectively).


CONCLUSIONS
VKORC1 polymorphisms seem not to be related with the thrombotic state of systemic and ocular Behçet's disease.
Department of Ophthalmology .
Helin Deniz
HD
Demir
Hüseyin
H
Ortak
Şemsettin
Ş
Şahin
Ömer
Ö
Ateş
Ismail
I
Benli
Ahmet
A
İnanır
D000328:Adult; D001528:Behcet Syndrome / Q000208:ethnology / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D031300:Retinal Vasculitis / Q000208:ethnology / Q000235:genetics*; D014421:Turkey / Q000208:ethnology; D064417:Vitamin K Epoxide Reductases / Q000235:genetics*
C578523:VKORC1 protein, human; D064417:Vitamin K Epoxide Reductases