Comparative pharmacokinetics study of three anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang by high performance liquid chromatography-mass spectrometry.
A specific and sensitive high performance liquid chromatography-mass spectrometric (HPLC-MS) method has been developed and validated for simultaneous determination of three anthraquinones of rhein, aloe-emodin and emodin in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang. The analytes were separated on a Kromaisl(®) C(18) column within a total running time of 12min with a mobile phase of methanol:ammonium acetate (3mM) (75:25, v/v). The calibration curves for all the anthraquinones showed good linearity in the measured range with correlation coefficient (r) higher than 0.9978. The precision, accuracy, recovery and stability were deemed acceptable. The method was successfully applied to the comparative pharmacokinetics study of the anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
Huan
H
Li
Hui
H
Guo
Li
L
Wu
Yongxin
Y
Zhang
Juan
J
Chen
Xiao
X
Liu
Hao
H
Cai
Kewei
K
Zhang
Baochang
B
Cai
D031841:Aconitum / Q000737:chemistry; D000284:Administration, Oral; D000818:Animals; D000880:Anthraquinones / Q000097:blood*; D030023:Asarum / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal; D004642:Emodin / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012250:Rheum / Q000737:chemistry; D012680:Sensitivity and Specificity; D013997:Time Factors
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C518327:aloe emodin; D004642:Emodin; C020491:rhein