Identification of TNF13b (BAFF) gene from grass carp (Ctenopharyngodon idella) and its immune response to bacteria and virus.
The TNF superfamily B cell activating factor (BAFF) is a central cytokine in several diseases. A BAFF gene has been cloned from grass carp (Ctenopharyngodon idella), analyzed its structure, and investigated its expression pattern in various tissues after Aeromonas hydrophila and Aquareovirus infection. The open reading frame of grass carp BAFF (gcBAFF) consists of 804 bases encoding 267 amino acids. Phylogenetic analysis shows the gcBAFF is most closely related to other teleost BAFFs with the highest similarity to zebrafish. RT-PCR analysis shows the gcBAFF transcript is expressed in a wide range of tissues with the highest expression in skin and spleen. Upon induction by A. hydrophila and Aquareovirus, its expression is significantly up-regulated in gill, liver, kidney, spleen and skin as compared to PBS injected fish. The association of increased BAFF expression after bacterial and viral infections suggests that it plays a potentially important role in immune system of fish.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
Narayan Prasad
NP
Pandit
Yubang
Y
Shen
Wenjing
W
Wang
Yong
Y
Chen
Jiale
J
Li
D016980:Aeromonas hydrophila / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D053264:B-Cell Activating Factor / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D020869:Gene Expression Profiling; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D010802:Phylogeny; D012087:Reoviridae / Q000276:immunology; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D053264:B-Cell Activating Factor; D018076:DNA, Complementary; D029941:Fish Proteins