Characterization of burden on growth due to the nutritional state of media and pre-induced gene expression.
Studies have shown that the production of unnecessary proteins burdens the cellular growth mainly due to allocation of cellular resources to unnecessary protein synthesis, thereby limiting the resources available for growth. In the current study, we focus on the effect of pre-induction and nutritional status of the medium on the burden imposed on growth due to the synthesis of unnecessary protein. Escherichia coli cells with different history were grown in a glycerol media with and without IPTG to characterize the burden imposed due to the synthesis of β-galactosidase. Effect of pre-induced lac operon on growth and β-galactosidase expression on lactose milieu was also investigated. The study demonstrates that pre-induction has a strong influence on the extent of burden and is sustained in several generations. Further, the burden was much lower in a rich media relative to that observed in a minimal media.
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, Maharashtra, India.
Pushkar
P
Malakar
K V
KV
Venkatesh
D003470:Culture Media / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007544:Isopropyl Thiogalactoside / Q000378:metabolism; D007763:Lac Operon; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D007544:Isopropyl Thiogalactoside; D001616:beta-Galactosidase