Intrasession reproducibility of RNFL thickness measurements using SD-OCT in eyes with keratoconus.
OBJECTIVE
To evaluate the intrasession reproducibility of the peripapillary retinal nerve fiber layer (RNFL) thickness measurements obtained by spectral-domain optical coherence tomography (SD-OCT) in eyes with keratoconus and normal eyes.


METHODS
Peripapillary RNFL thickness measurements with SD-OCT were repeated three times during the same visit using the eye tracker and retest function in one eye of each participant. Reproducibility was evaluated using within-subject standard deviation (Sw), coefficient of variation (CV), and intraclass correlation coefficient (ICC).


RESULTS
For the overall global RNFL thickness, the values of the three parameters were Sw (± 1.96 standard error) 1.43 ± 0.24, CV 1.28%, ICC (95% confidence interval) 0.969 (range: 0.947-0.983) in control eyes and Sw (± 1.96 standard error) from 1.41 ± 0.26 to 1.57 ± 0.34, CV from 1.18% to 1.37%, and ICC (95% confidence interval) from 0.951 (range: 0.909-0.976) to 0.977 (range: 0.938-0.993) in eyes with keratoconus.


CONCLUSIONS
Measurement of peripapillary RNFL thickness by SD-OCT shows a good intrasession reproducibility in eyes with keratoconus.
Eye Clinic, University of Catania, Catania, Italy.
Michele
M
Reibaldi
Maurizio G
MG
Uva
Teresio
T
Avitabile
Mario D
MD
Toro
Marco
M
Zagari
Cesare
C
Mariotti
Gilda
G
Cennamo
Alfredo
A
Reibaldi
Antonio
A
Longo
D000328:Adult; D000704:Analysis of Variance; D005260:Female; D006801:Humans; D007640:Keratoconus / Q000175:diagnosis*; D008297:Male; D009412:Nerve Fibers / Q000473:pathology*; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods / Q000592:standards*