Expressional profile of cardiac uncoupling protein-2 following myocardial ischemia reperfusion in losartan- and ramiprilat-treated rats.
OBJECTIVE
The aim of this study was to investigate the early changes of cardiac uncoupling protein-2 (UCP2) expression following myocardial ischemia reperfusion in rats chronically treated with ramiprilat and losartan.


METHODS
Male Wistar rats were assigned into seven groups (six in each): intact (control); sham-operated; nontreated rats subjected to ischemia and reperfusion (IR); ramiprilat-treated rats with (Ram+IR) and without ischemia (Ram); losartan treated with (Los+IR) and without ischemia (Los). Quantitative evaluation of UCP2 mRNA was carried out using real-time reverse transcription-polymerase chain reaction (RT-PCR). Mitochondria were isolated, and protein expression was quantified by Western blotting.


RESULTS
In IR group: UCP2 protein but not mRNA level was increased in the ischemic area of the left ventricle (LV) (172% ± 26.7, p < 0.001 vs. LV of control). Following acute myocardial IR, UCP2 protein levels was increased in the ischemic area of the LV but not in RV, suggesting the local effect of ischemia on UCP2 expression. IR-induced overexpression of UCP2 was suppressed by ramiprilat and losartan.


CONCLUSIONS
These findings suggest that losartan and ramiprilat can suppress UCP2 expression following myocardial IR, and by this mechanism may protect the myocardium against IR injury.
Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Physiology, Tarbiat Modares University, Tehran, Iran.
Fatemeh
F
Safari
Department of Physiology, Tarbiat Modares University, Tehran, Iran Department of Physiology and Pharmacology, Faculty of Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran.
Gholamreza
G
Bayat
Islamic Azad University, Arsanjan Branch, Fars, Iran.
Shahnaz
S
Shekarforoush
Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Seyedhossein
S
Hekmatimoghaddam
Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Zahra
Z
Anvari
Department of Biotechnology, Tarbiat Modares University, Tehran, Iran.
Mahdi Forouzandeh
MF
Moghadam
Department of Physiology, Tarbiat Modares University, Tehran, Iran Institute for Cognitive Science Studies (ICSS), Tehran, Iran fa.cardio@gmail.com.
Sohrab
S
Hajizadeh
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D020869:Gene Expression Profiling; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism; D019808:Losartan / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017257:Ramipril / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D017208:Rats, Wistar; D014158:Transcription, Genetic / Q000187:drug effects; D000071246:Uncoupling Protein 2
D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; C052549:ramiprilat; D019808:Losartan; D017257:Ramipril
Cardiac expression; UCP2; ischemia reperfusion; losartan; ramiprilat