Expression of immunoregulatory genes and its relationship to lead exposure and lead-mediated oxidative stress in wild ungulates from an abandoned mining area.
Lead (Pb) is a highly toxic metal that can induce oxidative stress and affect the immune system by modifying the expression of immunomodulator-related genes. The aim of the present study was to investigate the association between Pb exposure and the transcriptional profiles of some cytokines, as well as the relationship between Pb exposure and changes in oxidative stress biomarkers observed in the spleen of wild ungulates exposed to mining pollution. Red deer and wild boar from the mining area studied had higher spleen, liver, and bone Pb levels than controls, indicating a chronic exposure to Pb pollution. Such exposure caused a depletion of spleen glutathione levels in both species and disrupted the activity of antioxidant enzymes, suggesting the generation of oxidative stress conditions. Deer from the mining area also showed an induced T-helper (Th )-dependent immune response toward the Th 2 pathway, whereas boar from the mining area showed a cytokine profile suggesting an inclination of the immune response toward the Th 1 pathway. These results indicate that environmental exposure to Pb may alter immune responses in wild ungulates exposed to mining pollution. However, evidence of direct relationships between Pb-mediated oxidative stress and the changes detected in immune responses were not found. Further research is needed to evaluate the immunotoxic potential of Pb pollution, also considering the prevalence of chronic infectious diseases in wildlife in environments affected by mining activities.
Spanish Institute of Wildlife Research (IREC, CSIC-UCLM-JCCM), Ciudad Real, Spain. jaime.rodriguez@uclm.es
Jaime
J
Rodríguez-Estival
José M Pérez
JM
de la Lastra
Manuel E
ME
Ortiz-Santaliestra
Dolors
D
Vidal
Rafael
R
Mateo
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003670:Deer / Q000378:metabolism; D004781:Environmental Exposure / Q000032:analysis* / Q000706:statistics & numerical data; D004787:Environmental Pollution / Q000706:statistics & numerical data; D005260:Female; D015870:Gene Expression; D005978:Glutathione / Q000235:genetics / Q000378:metabolism; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D008099:Liver / Q000378:metabolism; D008297:Male; D008906:Mining; D018384:Oxidative Stress; D013154:Spleen / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D034421:Sus scrofa / Q000378:metabolism
D000975:Antioxidants; D007074:Immunoglobulin G; D053583:Interleukin-1beta; D015847:Interleukin-4; D007854:Lead; D007371:Interferon-gamma; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D005978:Glutathione