Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens.
The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability.
Abbott Molecular, Des Plaines, Illinois, USA.
Gavin A
GA
Cloherty
James
J
Rhoads
Thomas P
TP
Young
Neil T
NT
Parkin
Vera
V
Holzmayer
Lilly
L
Yuen
Carolyn
C
Mullen
D001483:Base Sequence; D017124:Conserved Sequence; D004279:DNA, Viral / Q000235:genetics*; D006509:Hepatitis B / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D019562:Viral Load / Q000379:methods*
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens