Norathyriol suppresses transformation in JB6 P+ cells by the inhibition of Akt.
BACKGROUND
Chemoprevention has been acknowledged as an important and practical strategy for the management of skin cancer. Norathyriol, a naturally occurring compound present in various plants, has a potent anticancer-promoting activity.


OBJECTIVE
The aim was to investigate the chemopreventive activity of norathyriol on JB6 P+ cells.


METHODS
A soft agar assay was used to detect the effect of norathyriol on cell transformation. The activator protein-1 (AP-1) transactivation activity was examined by the luciferase assay.


RESULTS
Norathyriol inhibited epidermal growth factor (EGF)- and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced neoplastic cell transformation in a dose-dependent manner. The activation of activator protein-1 was dose dependently suppressed by norathyriol treatment. Western blot data revealed that norathyriol attenuated the phosphorylation of Akt.


CONCLUSIONS
Norathyriol exerts a potent chemopreventive activity by inhibiting Akt activation in neoplastic cell transformation.
Department of Biochemistry, Guangdong Medical College, Dongguan, Guangdong, People's Republic of China, China. lijx313@vip.163.com
Jixia
J
Li
Xiangyong
X
Li
Zhiwei
Z
He
Keyuan
K
Zhou
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D051379:Mice; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014966:Xanthenes / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D047428:Protein Kinase Inhibitors; D018808:Transcription Factor AP-1; D014966:Xanthenes; C069053:norathyriol; D004815:Epidermal Growth Factor; D051057:Proto-Oncogene Proteins c-akt