Sequencing-based genotyping of mixed human papillomavirus infections by use of RipSeq software.
Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping assay. RipSeq Mixed provided rapid, sequencing-based HPV typing for single-type infections and coinfections with 2 types.
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA. keith.d.tardif@aruplab.com
Keith D
KD
Tardif
Keith E
KE
Simmon
Oyvind
O
Kommedal
Michael T
MT
Pyne
Robert
R
Schlaberg
D060085:Coinfection / Q000821:virology*; D019295:Computational Biology / Q000379:methods*; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
D004279:DNA, Viral