Roles of miR-1-1 and miR-181c in ventricular septal defects.
BACKGROUND
MicroRNAs (miRNAs) are endogenous noncoding RNAs of approximately 22 nucleotides in length that mediate post-transcriptional gene silencing by annealing to sequences in the 3'-untranslated region of target mRNAs.


METHODS
In this study, we analyzed 25 candidate miRNAs selected based on microarray data for cardiac tissue from individuals with congenital heart defects (CHDs) and from healthy control tissue.


RESULTS
This study identified specific changes in the miR-1-1 and miR-181c levels in human cardiac samples from individuals with ventricular septal defects (VSDs) relative to the levels in healthy control tissue. Increased levels of GJA1 and SOX9 were associated with the decreased expression of miR-1-1 in VSD patients, and increased miR-181c expression was correlated with downregulated BMPR2 levels. In addition, the results revealed that miR-1-1 and miR-181c directly regulate the expression of these predicted targets.


CONCLUSIONS
miR-1-1 and miR-181c are associated with the pathogenesis of VSDs.
Children Hospital of Fudan University, Shanghai 201102, China.
Jian
J
Li
Yinyin
Y
Cao
Xiao-jing
XJ
Ma
Hui-jun
HJ
Wang
Jin
J
Zhang
Xin
X
Luo
Weicheng
W
Chen
Yao
Y
Wu
Yingying
Y
Meng
Jing
J
Zhang
Yuan
Y
Yuan
Duan
D
Ma
Guo-ying
GY
Huang
D002648:Child; D002675:Child, Preschool; D005260:Female; D057809:HEK293 Cells; D006345:Heart Septal Defects, Ventricular / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D035683:MicroRNAs / Q000502:physiology*
C544547:MIRN1 microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs
BMPR2; GJA1; SOX9; Ventricular septal defect; miR-1-1; miR-181c