[Diversity of polyketide synthase genes (PKS) in metagenomic community of the freshwater sponge].
Screening of metagenomic DNA of microbial community, associated with Baikalian sponge Lubomirskia baicalensis, was made to show the presence of polyketide synthase genes (PKS). PKS enzymatic systems take part in synthesis of a great number of biologically-active substances. Cloning and sequencing of amplified products of the ketosynthase domain section of PKS gene cluster has revealed 15 fragments of PKS genes differing from each other's on 35-65% by aminoacid sequences. BLASTX analysis has shown that all these sequences belong to the KS-domains identified in various groups of microorganisms: alpha-, beta-, delta-Proteobacteria, Verrucomicrobia, Cyanobacteria, Chlorophyta. Some sequences were related to the genes which are taking part in biosynthesis of curacin A (CurI, CurJ), stigmatellin (StiC, StiG), nostophycin (NpnB), and cryptophycins (CrpB). The homology of the found sequences with those of the EMBL database varies within 50-82% confirming the presence in fresh-water sponge community the genes for synthesis of the new, yet not studied polyketide substances, possessing the biotechnological potential.
O V
OV
Kaliuzhnaia
N V
NV
Kulakova
B V
BV
Itskovich
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D000460:Chlorophyta / Q000235:genetics*; D005618:Fresh Water / Q000382:microbiology; D005798:Genes, Bacterial / Q000502:physiology; D017343:Genes, Plant / Q000502:physiology; D054892:Metagenome; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D048630:Polyketide Synthases / Q000235:genetics*; D011161:Porifera / Q000382:microbiology*; D012800:Siberia; D014871:Water Microbiology
D001426:Bacterial Proteins; D010940:Plant Proteins; D048630:Polyketide Synthases