Acute toxicity of acetylsalicylic acid to juvenile and embryonic stages of Danio rerio.
OBJECTIVE
The aim of this study was to compare the acute toxicity of acetylsalicylic acid to embryonic and juvenile stages of aquarium fish - zebrafish (Danio rerio), oxidative stress parameters and detoxifying enzyme.


METHODS
Tests were performed according to OECD No. 203 (Fish, acute toxicity test) and OECD No. 212 (Fish, short-term toxicity test on embryo and sac-fry stages) methodology.


RESULTS
The results showed the mean acetylsalicylic acid LC50 value to be 567.7 mg/L in juvenile zebrafish. The acute toxicity of acetylsalicylic acid for zebrafish embryos was 274.6 mg/L. Statistically significantly higher activity of GST was found in concentrations 340, 380 and 420 mg/L of acetylsalicylic acid. TBARS, GPx and GST didn't show statistically significant activity in tested concentrations of acetylsalicylic acid.


CONCLUSIONS
The results revealed a statistically significantly higher degree of sensitivity in the embryonic stages of zebrafish compared to its juveniles. Acetylsalicylic acid did not cause statistically significantly higher antioxidative defence in zebrafish.
Department of Veterinary Public Health and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. epraskova@vfu.cz
Eva
E
Praskova
Dana
D
Zivna
Stanislava
S
Stepanova
Marie
M
Sevcikova
Jana
J
Blahova
Petr
P
Marsalek
Zuzana
Z
Siroka
Eva
E
Voslarova
Zdenka
Z
Svobodova
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D001241:Aspirin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D008658:Inactivation, Metabolic / Q000502:physiology; D007928:Lethal Dose 50; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D060753:Water Quality; D015027:Zebrafish / Q000196:embryology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D014874:Water Pollutants, Chemical; D001241:Aspirin