A mutation 'hot spot' in the Schmallenberg virus M segment.
In the autumn of 2011, Schmallenberg virus (SBV), a novel orthobunyavirus of the Simbu serogroup, was identified by metagenomic analysis in Germany. SBV has since been detected in ruminants all over Europe, and investigations on phylogenetic relationships, clinical signs and epidemiology have been conducted. However, until now, only comparative sequence analysis of SBV genome segments with other species of the Simbu serogroup have been performed, and detailed data on the S and M segments, relevant for virus-host-cell interaction, have been missing. In this study, we investigated the S- and M-segment sequences obtained from 24 SBV-positive field samples from sheep, cattle and a goat collected from all over Germany. The results obtained indicated that the overall genome variability of SBV is neither regionally nor host species dependent. Nevertheless, we characterized for the first time a region of high sequence variability (a mutation 'hot spot') within the glycoprotein Gc encoded by the M segment.
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
Melina
M
Fischer
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
Bernd
B
Hoffmann
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
Katja V
KV
Goller
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
Dirk
D
Höper
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
Kerstin
K
Wernike
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
Martin
M
Beer
D000818:Animals; D001483:Base Sequence; D002044:Bunyaviridae Infections / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D005060:Europe; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D009154:Mutation; D016851:Orthobunyavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D014763:Viral Matrix Proteins