Detection of hypermethylated spastic paraplegia-20 in stool samples of patients with colorectal cancer.
BACKGROUND
Analysis of aberrant hypermethylation in stool DNA might provide a novel strategy for noninvasive detection of colorectal cancer.


OBJECTIVE
To explore the feasibility of detecting hypermethylation in Spastic paraplegia-20 promoter as a stool-based DNA marker for detection of colorectal cancer.


METHODS
We collected 96 tissue and stool samples from patients with colorectal cancer and 30 stool samples healthy individuals.


RESULTS
Hypermethylated Spastic paraplegia-20 occurs in 85.4% (82/96) of patients with colorectal cancer in the tissue samples. In the stool samples, the results indicate 80.2% (77/96) sensitivity and 100% (30/30) specificity of the test for detecting colorectal cancer by using the stool samples as a noninvasive method.


CONCLUSIONS
The study reveals that hypermethylation in Spastic paraplegia-20 promoter is a highly specific and sensitive biomarker for screening colorectal cancer in stool samples as a noninvasive method.
Department of Surgical Oncology the First Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, China.
Hao
H
Zhang
Yong-Chun
YC
Song
Cheng-Xue
CX
Dang
D000368:Aged; D018797:Cell Cycle Proteins; D015179:Colorectal Neoplasms / Q000150:complications / Q000175:diagnosis / Q000235:genetics; D004247:DNA / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics; D055088:Early Detection of Cancer; D005243:Feces / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010264:Paraplegia / Q000150:complications / Q000175:diagnosis / Q000235:genetics; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*
D018797:Cell Cycle Proteins; D011506:Proteins; C464787:SPART protein, human; D004247:DNA
Biomarker.; Colorectal cancer; Hypermethylation; SPG20; Screening; Stool DNA