[Effects of acupoint injection of autoblood on expression of pulmonary transcription factor GATA 3 and T-bet proteins and genes in asthma rats].
OBJECTIVE
To observe the effect of autoblood acupoint-injection (ABAI) on expression levels of pulmonary transacting T-cell-specific transcription factor GATA 3 (involving Th 2 cytokine expression), Th 1-specific T-box transcription factor T-box expressed in T-cells (T-bet) proteins and genes in asthmatic rats so as to explore its mechanisms underlying asthma relief.


METHODS
Forty-eight male SD rats were randomized into normal control (n = 8), model (n = 10), saline acupoint-injection (SAI, n = 10), ABAI (n = 10), and Dexamethasone (DXM, n = 10) groups. Asthma model was established during 28 days by 10% Ovalbumin + 10% aluminium hydroxide solution injection (i. p.) and vapourized 2% Ovalbumin inhaling for 14 days. For rats of the ABAI group, 0.4 mL autoblood was injected into the bilateral "Feishu" (BL 13) or "Shenshu" (BL 23) alternately, once every other day for six times. For rats of the DXM group, 50% DXM solution (0.5 mg/kg, i. p.) was given from the 17th day on after starting the modeling, once every other day for 11 days. Pulmonary GATA 3 and T-bet protein expression was detected by immunohistochemistry, and GATA 3 mRNA and T-bet mRNA expression detected by real time-PCR.


RESULTS
In comparison with the normal group, pulmonary GATA 3 protein and mRNA expression levels in the model group were up-regulated significantly (P < 0.01), while T-bet mRNA expression in the model group was down-regulated obviously (P < 0.01). Compared to the model group, GATA 3 protein and mRNA expression levels were down-regulated significantly in both ABAI and DXM groups (P < 0.01), while T-bet protein expression in ABAI group and T-bet mRNA expression in both ABAI and DXM groups were up-regulated significantly (P < 0.01, P < 0.05). No significant differences were found between model and SAI groups, and between ABAI and DXM groups in GATA 3 protein expression levels; and between ABAI and DXM groups in GATA 3 mRNA expression levels; between normal and model groups, and between SAI and ABAI groups in T-bet protein expression levels; between model and SAI groups and between ABAI and DXM groups in T-bet mRNA expression levels (P > 0.05). The ratio of GATA 3 mRNA/T-bet mRNA expression was significantly higher in the model group than in the normal group (P < 0.01), while obviously lower in the SAI, ABAI and DXM groups than in the model group (P < 0.05, P < 0.01). Additionally, the ratios of GATA 3 mRNA/T-bet mRNA in ABAI and DXM groups were comparable (P > 0.05).


CONCLUSIONS
Autoblood acupoint injection is comparable to DXM intraperitoneal injection in down-regulating asthma-induced increase of pulmonary GATA 3 protein and mRNA expression as well as ratio of GATA 3 mRNA/T-bet mRNA, and in up-regulating asthma-induced decrease of T-bet mRNA expression in asthma rats, which may contribute to their effects in relieving asthma.
Guangdong Hospital of Integrated Chinese and Western Medicine, Foshan 528200, China. mollc@163.com
Lü
L
Mo
Jun-Xiong
JX
Li
Julia
J
Kravitz
Chun-Zhi
CZ
Tang
Ze-Kai
ZK
Guo
Pei-Yong
PY
Li
Feng-Mei
FM
Guo
D015669:Acupuncture Points; D000818:Animals; D001249:Asthma / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D001769:Blood; D001804:Blood Transfusion, Autologous; D050990:GATA3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007267:Injections; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism; D000098815:T-bet Transcription Factor
D050990:GATA3 Transcription Factor; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor