miR-1289 and "Zipcode"-like Sequence Enrich mRNAs in Microvesicles.
Despite intensive studies, the molecular mechanisms by which the genetic materials are uploaded into microvesicles (MVs) are still unknown. This is the first study describing a zipcode-like 25 nucleotide (nt) sequence in the 3'-untranslated region (3'UTR) of mRNAs, with variants of this sequence present in many mRNAs enriched in MVs, as compared to their glioblastoma cells of origin. When this sequence was incorporated into the 3'UTR of a reporter message and expressed in a different cell type, it led to enrichment of the reporter mRNA in MVs. Critical features of this sequence are both a CUGCC core presented on a stem-loop structure and a miRNA-binding site, with increased levels of the corresponding miRNA in cells further increasing levels of mRNAs in MVs.
Department of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Mehmet Fatih
MF
Bolukbasi
Arda
A
Mizrak
Gokhan Baris
GB
Ozdener
Sibylle
S
Madlener
Thomas
T
Ströbel
Erdogan Pekcan
EP
Erkan
Jian-Bing
JB
Fan
Xandra O
XO
Breakefield
Okay
O
Saydam