Silibinin inhibits TPA-induced cell migration and MMP-9 expression in             thyroid and breast cancer cells.
Matrix metalloproteinases (MMPs) play an important role in cancer metastasis,             cell migration and invasion. Herein, we investigated the effects of silibinin             on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9             expression in thyroid and breast cancer cells. Our results revealed that the levels             of MMP-9 mRNA and protein expression were significantly increased by TPA but not             MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced             MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126,             and TPA-induced MMP-9 expression was significantly decreased. We also found that             TPA-induced cell migration and MMP-9 expression was significantly decreased by             silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited             by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced             cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid             and breast cancer cells.
Department of Surgery, Samsung Medical Center, Sungkyunkwan University                     School of Medicine, Gangnam-gu, Seoul 135-710, Republic of Korea.
Soo-Jin
SJ
Oh
Seung Pil
SP
Jung
Jeonghun
J
Han
Sangmin
S
Kim
Jee Soo
JS
Kim
Seok Jin
SJ
Nam
Jeong Eon
JE
Lee
Jung-Han
JH
Kim
D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D002070:Butadienes / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000235:genetics; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation; D000077385:Silybin; D012838:Silymarin / Q000008:administration & dosage*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013964:Thyroid Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*
D002070:Butadienes; D009570:Nitriles; D012838:Silymarin; C113580:U 0126; D000077385:Silybin; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate