Acidulated phosphate fluoride application changes the protein composition of human acquired enamel pellicle.
We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF solution, were immersed in human saliva for pellicle formation. The adsorbed proteins were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Fifty-six proteins were identified, 12 exclusive to APF and 11 to phosphoric acid. APF decreased the concentration of histatin-1, but increased the concentration of S100-A9, which is confirmed by immunoblotting. The findings suggest that APF application changes the acquired enamel pellicle composition.
Piracicaba Dental School, University of Campinas, UNICAMP, Piracicaba, Brazil.
N
N
Masson
R R
RR
Domingues
J A
JA
Cury
A F
AF
Paes Leme
D000149:Acidulated Phosphate Fluoride / Q000494:pharmacology*; D000818:Animals; D002124:Calcium Fluoride / Q000494:pharmacology*; D040502:Calgranulin B / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid; D003746:Dental Enamel Proteins / Q000032:analysis / Q000187:drug effects*; D044622:Dental Pellicle / Q000737:chemistry* / Q000187:drug effects*; D054808:Histatins / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods
D040502:Calgranulin B; D003746:Dental Enamel Proteins; D054808:Histatins; D000149:Acidulated Phosphate Fluoride; D002124:Calcium Fluoride