Inhibitory effect on NO production of triterpenes from the fruiting bodies of Ganoderma lucidum.
Four new lanostane triterpenes, butyl lucidenate P (1), butyl lucidenate D(2) (2), butyl lucidenate E(2) (3) and butyl lucidenate Q (4) along with 11 known compounds (5-15) were isolated from the fruiting bodies of Ganoderma lucidum. Their chemical structures were established mainly by 1D and 2D NMR techniques and mass spectrometry. Their anti-inflammatory activity was evaluated against LPS-induced NO production in macrophage RAW 264.7 cells. Compounds 1, 3, 4, 9, 10 and 15 showed inhibitory potency with IC(50) values of 7.4, 6.4, 4.3, 9.4, 9.2 and 4.5 μM, respectively. Compounds 1, 3 and 15 dose-dependently reduced the LPS-induced iNOS expressions. Preincubation of cell with 1, 3 and 15 significantly suppressed LPS-induced expression of COX-2 protein.
College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
Nguyen The
NT
Tung
To Dao
TD
Cuong
Tran Manh
TM
Hung
Jeong Hyung
JH
Lee
Mi Hee
MH
Woo
Jae Sue
JS
Choi
Jaewang
J
Kim
Sung Ho
SH
Ryu
Byung Sun
BS
Min
D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D020940:Reishi / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D014315:Triterpenes; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2