DNA origami nanopillars as standards for three-dimensional superresolution microscopy.
Nanopillars are promising nanostructures composed of various materials that bring new functionalities for applications ranging from photovoltaics to analytics. We developed DNA nanopillars with a height of 220 nm and a diameter of ~14 nm using the DNA origami technique. Modifying the base of the nanopillars with biotins allowed selective, upright, and rigid immobilization on solid substrates. With the help of site-selective dye labels, we visualized the structure and determined the orientation of the nanopillars by three-dimensional fluorescence superresolution microscopy. Because of their rigidity and nanometer-precise addressability, DNA origami nanopillars qualify as scaffold for the assembly of plasmonic devices as well as for three-dimensional superresolution standards.
Institut für Physikalische und Theoretische Chemie, Technische Universität Braunschweig, Hans-Sommer-Strasse 10, 38106 Braunschweig, Germany.
Jürgen J
JJ
Schmied
Carsten
C
Forthmann
Enrico
E
Pibiri
Birka
B
Lalkens
Philipp
P
Nickels
Tim
T
Liedl
Philip
P
Tinnefeld
D004247:DNA / Q000737:chemistry*; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
D004247:DNA