A Rab10-dependent mechanism for polarized basement membrane secretion during organ morphogenesis.
Basement membranes (BMs) are specialized extracellular matrices that are essential for epithelial structure and morphogenesis. However, little is known about how BM proteins are delivered to the basal cell surface or how this process is regulated during development. Here, we identify a mechanism for polarized BM secretion in the Drosophila follicle cells. BM proteins are synthesized in a basal endoplasmic reticulum (ER) compartment from localized mRNAs and are then exported through Tango1-positive ER exit sites to basal Golgi clusters. Next, Crag targets Rab10 to structures in the basal cytoplasm, where it restricts protein delivery to the basal surface. These events occur during egg chamber elongation, a morphogenetic process that depends on follicle cell planar polarity and BM remodeling. Significantly, Tango1 and Rab10 are also planar polarized at the basal epithelial surface. We propose that the spatial control of BM production along two tissue axes promotes exocytic efficiency, BM remodeling, and organ morphogenesis.
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA.
David W
DW
Lerner
Darcy
D
McCoy
Adam J
AJ
Isabella
Anthony P
AP
Mahowald
Gary F
GF
Gerlach
Thymur A
TA
Chaudhry
Sally
S
Horne-Badovinac
D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism*; D016764:Cell Polarity; D024141:Collagen Type IV / Q000378:metabolism; D004330:Drosophila / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D009024:Morphogenesis; D038081:Organogenesis; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000235:genetics / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism
D024141:Collagen Type IV; D029721:Drosophila Proteins; C109766:tgo protein, Drosophila; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase; D020558:GTP Phosphohydrolases; D020559:Monomeric GTP-Binding Proteins; C579344:Rab10 protein, Drosophila; D020691:rab GTP-Binding Proteins