Synthesis of multifunctional bioresponsive polymers for the management of chronic wounds.
Novel multifunctional bioresponsive gelatin and alginate based hydrogels with in-built antioxidant regenerating system and antimicrobial properties were successfully synthesized. These hydrogels are based on the versatile reactions catalyzed by cellobiose dehydrogenase (CDH). CDH uses cellobiose and cello-oligosacharides as electron donors to reduce oxidized phenolic antioxidants, quinones, or molecular oxygen to H₂O₂ (a well-known antimicrobial agent). The antioxidant regenerating system consisting of CDH and cellobiose increased the ability of catechol to quench nitric oxide (NO), superoxide (O₂⁻) and hydroxyl radicals (OH•) in solution and when incorporated into hydrogels. The CDH loaded into the hydrogels free of oxidized phenolic antioxidants and quinones reduced molecular to H₂O₂ resulting in the complete inhibition of the growth of Stapylococcus aeureus, Bacillus subtilis, Pseudomonas putida, Escherichia coli and Cellulomonasmicrobium cellulans. This study therefore presents a new concept for synthesizing multifunctional bioresponsive chronic wound dressing polymers with in-built continuous antioxidant system able to continuously quench [reactive oxygen species (ROS) and reactive nitrogen species (RNOS)], and antimicrobial properties able to prevent microbial colonization of wound.
Department of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/1, A-8010 Graz, Austria. g.nyanhongo@tugraz.at
Gibson S
GS
Nyanhongo
Christoph
C
Sygmund
Roland
R
Ludwig
Endry Nugroho
EN
Prasetyo
Georg M
GM
Guebitz
D000464:Alginates; D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D047070:Bandages, Hydrocolloid; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D001704:Biopolymers / Q000096:biosynthesis* / Q000737:chemistry / Q000627:therapeutic use; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D002908:Chronic Disease; D005780:Gelatin; D006801:Humans; D020100:Hydrogels; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D008422:Materials Testing; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014945:Wound Healing; D014947:Wounds and Injuries / Q000382:microbiology / Q000628:therapy*
D000464:Alginates; D000890:Anti-Infective Agents; D000975:Antioxidants; D001672:Biocompatible Materials; D001704:Biopolymers; D020100:Hydrogels; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D005780:Gelatin; D006861:Hydrogen Peroxide; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase