A low-cost intracellular delivery system based on microbubble and high gravity field.
In this paper, we developed a low-cost intracellular delivery system based on microbubble and high gravity field. We successfully delivered FITC-Dextran (40kD) into hard-to-deliver THP-1 cells. The results showed that our method achieved high delivery efficiency up to 80%. It was found that the delivery efficiency and cell viability were closely related to the centrifuge speed. We speculated that the burst of microbubbles causes transient pore opening thus increasing the chance of biomolecules entering cells. This fast, low-cost and easy-to-operate protocol is very promising for delivering therapeutic genes and drugs into any cells which do not actively take up extracellular materials. This method is most effective for in-vitro delivery, but after delivery, treated cells might be injected back to human for in-vivo imaging.
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, Canada.
Chuan
C
He
Quanrong
Q
Gu
Min
M
Huang
Xiaoyan
X
Yang
James
J
Xing
Jie
J
Chen
D002460:Cell Line; D003911:Dextrans / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D018471:Hypergravity; D008264:Macrophages / Q000737:chemistry*; D045423:Microbubbles
D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate