Pathogen typing in the genomics era: MLST and the future of molecular epidemiology.
Multi-locus sequence typing (MLST) is a high-resolution genetic typing approach to identify species and strains of pathogens impacting human health, agriculture (animals and plants), and biosafety. In this review, we outline the general concepts behind MLST, molecular approaches for obtaining MLST data, analytical approaches for MLST data, and the contributions MLST studies have made in a wide variety of areas. We then look at the future of MLST and their relative strengths and weaknesses with respect to whole genome sequence typing approaches that are moving into the research arena at an ever-increasing pace. Throughout the paper, we provide exemplar references of these various aspects of MLST. The literature is simply too vast to make this review comprehensive, nevertheless, we have attempted to include enough references in a variety of key areas to introduce the reader to the broad applications and complications of MLST data.
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal. mlosada323@gmail.com
Marcos
M
Pérez-Losada
Patricia
P
Cabezas
Eduardo
E
Castro-Nallar
Keith A
KA
Crandall
D000818:Animals; D001424:Bacterial Infections / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001499:Bayes Theorem; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D006801:Humans; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA