Viable circulating tumor cell enrichment by flexible micro spring array.
We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 10(4) enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.
Department of Bioengineering and Material Research Institute, The Pennsylvania State University, University Park, PA 16802, USA.
Ramdane A
RA
Harouaka
Ming-Da
MD
Zhou
Tim Y
TY
Yeh
Waleed J
WJ
Khan
Jeffery
J
Allerton
Si-Yang
SY
Zheng
D000925:Anticoagulants / Q000008:administration & dosage; D049109:Cell Proliferation; D004867:Equipment Design; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D009360:Neoplastic Cells, Circulating
D000925:Anticoagulants