Effects of methanol on a methanol-tolerant bacterial lipase.
Methanol is often employed in biocatalysis with the purpose of increasing substrates solubility or as the acyl acceptor in transesterification reactions, but inhibitory effects are observed in several cases. We have studied the influence of methanol on the catalytic activity and on the conformation of the lipase from Burkholderia glumae, which is reported to be highly methanol tolerant if compared with other lipases. We detected highest activity in the presence of 50-70 % methanol. Under these conditions, however, the enzyme stability is perturbed, leading to gradual protein unfolding and finally to aggregation. These results surmise that, for this lipase, methanol-induced deactivation does not depend on inhibition of catalytic activity but rather on negative effects on the conformational stability of the catalyst.
Department of Biotechnology and Biosciences, State University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.
Carlo
C
Santambrogio
Francesco
F
Sasso
Antonino
A
Natalello
Stefania
S
Brocca
Rita
R
Grandori
Silvia Maria
SM
Doglia
Marina
M
Lotti
D019117:Burkholderia / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D011487:Protein Conformation
D004791:Enzyme Inhibitors; D008049:Lipase; D000432:Methanol