Influence of two types of organic matter on interaction of CeO2 nanoparticles with plants in hydroponic culture.
An important aspect in risk assessment of nanoparticles (NPs) is to understand their environmental interactions. We used hydroponic plant cultures to study nanoparticle-plant-root interaction and translocation and exposed wheat and pumpkin to suspensions of uncoated CeO2-NP for 8d (primary particle size 17-100 nm, 100 mg L(-1)) in the absence and presence of fulvic acid (FA) and gum arabic (GA) as representatives of different types of natural organic matter. The behavior of CeO2-NPs in the hydroponic solution was monitored regarding agglomeration, sedimentation, particle size distribution, surface charge, amounts of root association, and translocation into shoots. NP-dispersions were stable over 8d in the presence of FA or GA, but with growing plants, changes in pH, particle agglomeration rate, and hydrodynamic diameter were observed. None of the plants exhibited reduced growth or any toxic response during the experiment. We found that CeO2-NPs translocated into pumpkin shoots, whereas this did not occur in wheat plants. The presence of FA and GA affected the amount of CeO2 associated with roots (pure>FA>GA) but did not affect the translocation factor. Additionally, we could confirm via TEM and SEM that CeO2-NPs adhered strongly to root surfaces of both plant species.
Soil Protection, Institute of Terrestrial Ecosystems, ETH-Zurich, Universitaetstrasse 16, CH-8092 Zurich, Switzerland.
Franziska
F
Schwabe
Rainer
R
Schulin
Ludwig K
LK
Limbach
Wendelin
W
Stark
Diane
D
Bürge
Bernd
B
Nowack
D002563:Cerium / Q000378:metabolism / Q000633:toxicity*; D028464:Cucurbita / Q000187:drug effects / Q000502:physiology*; D018527:Hydroponics; D053758:Nanoparticles / Q000032:analysis / Q000633:toxicity*; D014908:Triticum / Q000187:drug effects / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D002563:Cerium; C030583:ceric oxide