Suppression of LPS-induced inflammatory responses by gossypol in RAW 264.7 cells and mouse models.
Gossypol, a yellowish polyphenolic compound originally from cotton plant, has been known to exert a potential for anti-cancer, anti-inflammatory and other important therapeutic activities. The purpose of this investigation was to determine the protection of gossypol on inflammation in Lipopolysaccharide (LPS) stimulated RAW 264.7 cells and LPS induced in vivo lung injury model. The effects of gossypol on pro-inflammatory cytokines and signaling pathways were evaluated by enzyme-linked immunosorbent assay and Western blot. The results showed that gossypol significantly inhibited the production of LPS-induced TNF-α, IL-6 and IL-1β both in vitro and vivo. Furthermore, gossypol blocked the phosphorylation of IκBα protein, p65, p38, c-Junterminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells. From the in vivo study, it was observed that gossypol attenuated lung histopathologic changes in mouse models. The present data suggest that gossypol suppresses the inflammation in vitro and vivo, and may be a potential therapeutic candidate for the treatment of inflammatory disorders.
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Meixia
M
Huo
Ruijie
R
Gao
Lanxiang
L
Jiang
Xiurui
X
Cui
Lingxin
L
Duan
Xuming
X
Deng
Shuang
S
Guan
Jingyuan
J
Wei
Lanan Wassy
LW
Soromou
Haihua
H
Feng
Gefu
G
Chi
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D003368:Gossypium / Q000276:immunology*; D006072:Gossypol / Q000008:administration & dosage* / Q000009:adverse effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D055370:Lung Injury / Q000188:drug therapy* / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D006072:Gossypol