Effect of ghrelin on inflammatory response in lung contusion.
The purpose of this study was to investigate the effects of ghrelin on inflammatory response and tissue damage following trauma-induced acute lung injury. Thirty male wistar albino rats (300-400 g) were randomly assigned into three groups: control group (n = 6), lung contusion plus saline (saline-treated, n = 12), and lung contusion plus ghrelin (ghrelin-treated, n = 12). Saline- or ghrelin-treated traumatic rats were sacrificed at two time points (24 and 72 hours) after lung contusion. Blood was collected for the analysis of serum adenosine deaminase (ADA). Tissue transforming growth factor-beta 1 (TGF-β1) and matrix metalloproteinase-2 (MMP-2) levels were measured by enzyme-linked immunosorbent assay and histopathological examination was performed on the lung tissue samples. Our results indicated that ghrelin significantly reduced morphologic damages. Serum ADA activities were significantly decreased after lung contusion and this decline started early with ghrelin treatment. TGF-β1 and MMP-2 levels in lung tissue were elevated at 72 hours after lung contusion and treatment with ghrelin significantly increased TGF-β1 level and reduced MMP-2 level. In conclusion, our study demonstrates that acute lung injury initiated proinflammatory responses and ghrelin administration showed an anti-inflammatory effect in lung contusion.
Department of Biochemistry, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, Turkey. berrak_guven@hotmail.com
Berrak
B
Guven
Mertol
M
Gokce
Ozkan
O
Saydam
Murat
M
Can
Sibel
S
Bektas
Serhan
S
Yurtlu
D055371:Acute Lung Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000243:Adenosine Deaminase / Q000097:blood; D000818:Animals; D054439:Ghrelin / Q000008:administration & dosage*; D007249:Inflammation / Q000517:prevention & control; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014949:Wounds, Nonpenetrating / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D054439:Ghrelin; D053773:Transforming Growth Factor beta1; D020778:Matrix Metalloproteinase 2; D000243:Adenosine Deaminase