A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.
The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.
Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), National Centre of Competence in Research (NCCR) in Chemical Biology, 1015 Lausanne, Switzerland.
Gražvydas
G
Lukinavičius
Keitaro
K
Umezawa
Nicolas
N
Olivier
Alf
A
Honigmann
Guoying
G
Yang
Tilman
T
Plass
Veronika
V
Mueller
Luc
L
Reymond
Ivan R
IR
Corrêa
Zhen-Ge
ZG
Luo
Carsten
C
Schultz
Edward A
EA
Lemke
Paul
P
Heppenstall
Christian
C
Eggeling
Suliana
S
Manley
Kai
K
Johnsson
D002460:Cell Line; D004926:Escherichia coli; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D018613:Microscopy, Confocal; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013237:Stereoisomerism
D005456:Fluorescent Dyes; D011506:Proteins