Gene delivery by microfluidic flow-through electroporation based on constant DC and AC field.
Electroporation is one of the most widely used physical methods to deliver exogenous nucleic acids into cells with high efficiency and low toxicity. Conventional electroporation systems typically require expensive pulse generators to provide short electrical pulses at high voltage. In this work, we demonstrate a flow-through electroporation method for continuous transfection of cells based on disposable chips, a syringe pump, and a low-cost power supply that provides a constant voltage. We successfully transfect cells using either DC or AC voltage with high flow rates (ranging from 40 µl/min to 20 ml/min) and high efficiency (up to 75%). We also enable the entire cell membrane to be uniformly permeabilized and dramatically improve gene delivery by inducing complex migrations of cells during the flow.
Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47906, USA. tgeng@ purdue.edu
Tao
T
Geng
Yihong
Y
Zhan
Chang
C
Lu
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D044085:Microfluidics