Cytotoxic constituents of ethyl acetate fraction from Dianthus superbus.
The ethyl acetate fraction (EE-DS) from Dianthus superbus was found to possess the cytotoxic activity against cancer cells in previous study. To investigate cytotoxic constituents, the bioassay-guided isolation of compounds from EE-DS was performed. Two dianthramides (1 and 2), three flavonoids (3-5), two coumarins (6 and 7) and three other compounds (8-10) were obtained. Structures of isolated compounds were identified by spectroscopic analysis. Cytotoxicity of the compounds against HepG2 cells was evaluated. Compound 1 showed the strongest cytotoxicity, compounds 10, 4, 3 and 5 had moderate cytotoxicity.
a Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences , Wuhan , 430071 , P.R. China.
Chengli
C
Ding
Wu
W
Zhang
Jie
J
Li
Jiachuan
J
Lei
Jianqing
J
Yu
D000085:Acetates / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry; D029749:Dianthus / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*
D000085:Acetates; D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts; C007650:ethyl acetate