Moniliella byzovii sp. nov., a chlamydospore-forming black yeast isolated from flowers.
Yeasts of the genus Moniliella were isolated from 651 flower samples collected in Vietnam, using an enrichment medium containing 50 % glucose. Species of the genus Moniliella were found in 5 % of the samples and 54 strains were isolated. The strains were identified based on D1/D2 LSU rRNA gene sequences as M. megachiliensis (15 strains), M. dehoogii (14 strains), and M. mellis (2 strains). The remaining 23 strains could not be reliably placed under any known species. Among them, 12 strains isolated from flowers of Ipomoea pes-caprae and Calotropis gigantea were peculiar for the intensive formation of chlamydospores. These strains could be subdivided into pigmented and non-pigmented groups. Both groups were identical in PCR fingerprints generated with primer (GAC)5 and in D1/D2 and ITS sequences. The yeast was closely related to M. fonsecae but differed from the latter by 52 nt (or 10.3 % of divergence) in the D1/D2 sequence and 71 nt (or 16.9 % of divergence) in the ITS sequence. The name Moniliella byzovii sp. nov. is proposed for this novel species. The type strain is TBY 2041.7(T) = CBS 12757(T) = NRRL Y-63661(T). The MycoBank number is MB 803186.
Department of Microbiology, Food Industries Research Institute, 301-Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
Vu Nguyen
VN
Thanh
Department of Microbiology, Food Industries Research Institute, 301-Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
Dinh Duc
DD
Hien
Department of Microbiology, Food Industries Research Institute, 301-Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
Tran Thi
TT
Thom
D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D035264:Flowers / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D010858:Pigmentation; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal; D014744:Vietnam
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer