Transplantation of inbred adipose-derived stromal cells in rats with plasma gel containing fragmin/protamine microparticles and FGF-2.
Fragmin/protamine microparticles (F/P MPs) have been used as a cell carrier for adipose-derived stromal cells (IR-ASCs) in inbred male Fisher 344 rats, and for preservation and controlled-release of fibroblast growth factor (FGF)-2 and various cytokines in inbred rat plasma (IRP)-DMEM (Dulbecco's modified Eagle's medium) gel. In this study, we investigated the capability of an IRP-DMEM gel containing F/P MPs and/or FGF-2, as a three-dimensional (3D)-culture, to expand IR-ASCs. We found that IR-ASCs grow faster under 3D-culture conditions in low IRP (3%)-DMEM gel containing F/P MPs and FGF-2 without any animal serum than those under 2D-culture in low inbred rat serum (3%)-DMEM with F/P MPs and FGF-2. About 0.3 mL of IR-ASCs (about 4,000,000 cells mL⁻¹) grown in IRP (6%)-DMEM gel containing F/P MPs and FGF-2 disappeared 8 days after subcutaneous injection in rats, suggesting that they are rapidly biodegradable. The number of large (diameter ≥200 μm or containing ≥100 erythrocytes), medium (diameter = 20-200 μm or containing 10-100 erythrocytes) and small (diameter ≤20 μm or containing 1-10 erythrocytes) capillaries after injection with IR-ASCs in an IRP-DMEM gel containing both F/P MPs and FGF-2, as well as the thickness of tissue granulation per microphotograph at the injected site, was significantly higher than those after injection with IR-ASCs in an IRP-DMEM gel containing either FGF-2 or F/P MPs. Thus, IRP-DMEM gel containing F/P MPs and FGF-2 are useful and safe IR-ASC carriers that facilitate cell proliferation, vascularization, and tissue granulation locally at injection sites.
Department of Plastic Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Yuki
Y
Sumi
Masayuki
M
Ishihara
Satoko
S
Kishimoto
Megumi
M
Takikawa
Takashi
T
Doumoto
Ryuichi
R
Azuma
Shingo
S
Nakamura
Hidemi
H
Hattori
Masanori
M
Fujita
Tomoharu
T
Kiyosawa
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D055252:Cell-Derived Microparticles / Q000737:chemistry; D017985:Dalteparin / Q000737:chemistry; D003692:Delayed-Action Preparations; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D005782:Gels; D006097:Granulation Tissue / Q000254:growth & development; D008297:Male; D008422:Materials Testing; D018919:Neovascularization, Physiologic; D010949:Plasma / Q000737:chemistry; D011479:Protamines / Q000737:chemistry; D051381:Rats; D011916:Rats, Inbred F344; D018874:Spheroids, Cellular / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology* / Q000637:transplantation*; D023822:Tissue Engineering
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D005782:Gels; D011479:Protamines; D016222:Fibroblast Growth Factor 2; D017985:Dalteparin