[Systematic study on QAMS method for quality control of Panax notoginseng].
OBJECTIVE
To establish a quantitative method of multi-components by single marker (QAMS) for determining ginsenoside Rg1, Rb1, Rd, Re and notoginsenoside R1 for the purpose of the quality control of Panax notoginseng.


METHODS
The relative correction factors (RCFs) between the five active saponins were determined by HPLC-DAD. With any of the five consituents as reference, a QAMS method was established for detect the quantitation of the other four consituents. The durability of the method was evaluated with five different HPLC instruments, five different Cis18 chromatographic columns and four detective wavelengths. Subsequently, the new QAMS method was used to determine the contents of five saponins contained in 43 batches of notoginseng samples, and compare with external standard methods, in order to evaluate the accuracy of the QAMS method.


RESULTS
When the five saponins were taken for reference, there was no significant difference between the contents of Rg1, Rb1, Rd, Re and R1 contained in the 43 batches of medicines calculated by the QAMS method (Wf) and the content determination result of the external standard method (Ws). The ratio of their results was (Ws/Wr) (94.02 +/- 2.11)%-(99.75 +/- 0.79)%, suggesting that the method was highly accurate. Their relative correction factors showed good durability, ranging between 0.42%-3.7%, 0.52%-3.5% and 0.79%-4.9%, respectively, with different chromatographic columns, different instruments and different detective wavelengths. The relative retention value method could be adopted for accurately position the chromatographic peak of the five consituents, with their values ranging between 0.18%-13%.


CONCLUSIONS
An accurate, rapid and highly durable QAMS method is established for simultaneous determination and location of five saponins, so as to provide reliable basis for the application of the QAMS method in quality control of traditional Chinese medicines.
Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. wangchaoqun8676@live.cn
Chao-Qun
CQ
Wang
Xiu-Hong
XH
Jia
Jii
J
Chen
Xin-Yue
XY
Xiao
Xuani
X
Wang
Shao-Qing
SQ
Cai
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D036145:Ginsenosides / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D015394:Molecular Structure; D053723:Panax notoginseng / Q000737:chemistry*; D011786:Quality Control
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides