[The expression and modulatory role of reprogramming markers on the in vivo regeneration of dental pulp and periodontal ligament tissue after injury].
OBJECTIVE
The purpose of this study was to investigate the expression of reprogramming markers including Oct-4, Sox2 and c-Myc in rat dental pulp and periodontal ligament tissues undergoing tissue regeneration after injury.


METHODS
Lewis rat model with dental pulp and periodontal ligament injury was created. HE staining was applied to investigate the structure of newly formed dentin-pulp complex and periodontium. The distribution of Oct-4, Sox2 and c-Myc was determined by immunofluorescent staining.


RESULTS
HE staining showed that newly formed dentin-pulp complex and periodontium was constructed 4 weeks after injury. Immunofluorescent staining revealed Oct-4, Sox2 and c-Myc signal was activated in the newly formed dental pulp and periodontal ligament tissue after injury compared with the normal ones. Oct-4 and Sox2 showed similar expression pattern.


CONCLUSIONS
Oct-4, Sox2 and c-Myc signaling may play an important modulatory role in the dental tissue regeneration after injury. Oct-4 and Sox2 may have operative interaction whereas c-Myc may play independent role during this process.
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, Guangdong Province, China. liulu32@mail.sysu.edu.cn
Lu
L
Liu
Xi
X
Wei
Jun-qi
JQ
Ling
Zheng-jun
ZJ
Peng
Li-ping
LP
Wu
D000818:Animals; D003782:Dental Pulp; D003804:Dentin; D010513:Periodontal Ligament; D010519:Periodontium; D051381:Rats; D011917:Rats, Inbred Lew; D012038:Regeneration; D014945:Wound Healing