Analysis of food polyphenols by ultra high-performance liquid chromatography coupled to mass spectrometry: an overview.
Phenolic compounds, which are widely distributed in plant-derived foods, recently attracted much attention due to their health benefits, so their determination in food samples is a topic of increasing interest. In the last few years, the development of chromatographic columns packed with sub-2μm particles and the modern high resolution mass spectrometry (MS) have opened up new possibilities for improving the analytical methods for complex sample matrices, such as ingredients, foods and biological samples. In addition, they have emerged as an ideal tool for profiling complex samples due to its speed, efficiency, sensitivity and selectivity. The present review addresses the use of the improved liquid chromatography (LC), ultra-high performance LC (UHPLC), coupled to MS or tandem MS (MS/MS) as the detector system for the determination of phenolic compounds in food samples. Additionally, the different strategies to extract, quantify the phenolic compounds and to reduce the matrix effect (%ME) are also reviewed. Finally, a briefly outline future trends of UHPLC-MS methods is commented.
Food Technology Department, XaRTA-TPV, Escola Tècnica Superior d'Enginyeria Agrària, Universitat de Lleida, Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain. motilva@tecal.udl.es
Maria-José
MJ
Motilva
Aida
A
Serra
Alba
A
Macià
D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010944:Plants / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis*
D059808:Polyphenols