Attenuated adiposopathy in perivascular adipose tissue compared with subcutaneous human adipose tissue.
BACKGROUND
We hypothesized that human perivascular and subcutaneous adipose tissues hold distinct phenotypic signatures. We also evaluated the impact of clinical parameters on the adipose phenotype. Our overall goal is to understand the determinants of adipose biology so that this tissue can be manipulated therapeutically to lessen peripheral vascular disease.


METHODS
Perivascular and subcutaneous adipose tissues were collected from patients undergoing lower-extremity amputation (n = 27) and protein assayed for proinflammatory mediators (ie, interleukin 6, interleukin 8, leptin, tumor necrosis factor α, monocyte chemoattractant protein-1, and resistin), atheroprotective adiponectin, and the fibrinolysis inhibitor plasminogen activator inhibitor-1.


RESULTS
Leptin (2.7-fold, P = .015), TNF-α (2.2-fold, P = .013), MCP-1 (1.5-fold, P = .047), and adiponectin (1.8-fold, P = .004) were more abundant in subcutaneous vs perivascular adipose tissue. Age positively correlated with perivascular adipose tissue PAI-1 expression (β = .64, P = .042), and hyperlipidemia negatively correlated with perivascular adiponectin (β = -1.18, P = .039).


CONCLUSIONS
Human perivascular and subcutaneous adipose tissues hold distinct phenotypic signatures. In amputation patients, the subcutaneous adipose tissue proinflammatory phenotype was relatively attenuated in perivascular adipose tissue.
Division of Vascular and Endovascular Surgery, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA.
Christine R
CR
Mauro
Godfrey
G
Ilonzo
Binh T
BT
Nguyen
Peng
P
Yu
Ming
M
Tao
Ian
I
Gao
Michael A
MA
Seidman
Louis L
LL
Nguyen
C Keith
CK
Ozaki
D052242:Adiponectin / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology*; D000367:Age Factors; D000368:Aged; D000671:Amputation, Surgical; D015415:Biomarkers / Q000378:metabolism*; D001808:Blood Vessels; D018932:Chemokine CCL2 / Q000378:metabolism; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D035002:Lower Extremity; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D052243:Resistin / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D052242:Adiponectin; D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D007378:Interleukins; D020738:Leptin; D017395:Plasminogen Activator Inhibitor 1; C499624:RETN protein, human; D052243:Resistin; C051321:SERPINE1 protein, human; D014409:Tumor Necrosis Factor-alpha
Adipokine; Adipose; Perivascular