Dynamic change of TNIK in response to tumor necrosis factor alpha in a TRAF2-dependent manner.
TNIK (TRAF2- and NCK-interacting kinase) was named because of its association with TRAF2 (tumor necrosis factor receptor-associated factor 2). But the relationship between TNIK and TRAF2 is still elusive, in addition to which the involvement of TNIK in JNK activation by TNFα hints that there maybe a linkage between TNIK and TRAF2. In this work, we illustrated that TNIK protein levels were dynamic in response to TNFα stimulation in an ubiquitin-dependent manner. Further study showed that TRAF2 negatively modulated the levels of TNIK by regulating the ubiquitin conjugation. In conclusion, our data may give evidence that dynamic change of TNIK offers a way to protect cells from outside stimulus.
Xinyuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Mailbox 539, Hangzhou 310018, Zhejiang, People's Republic of China.
Jinghua
J
Gui
Zhenhai
Z
Li
Xiumei
X
Zhou
D017209:Apoptosis / Q000235:genetics*; D000081410:Germinal Center Kinases; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology; D016328:NF-kappa B / Q000502:physiology; D010446:Peptide Fragments; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism / Q000502:physiology*; D047992:TNF Receptor-Associated Factor 2 / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D025801:Ubiquitin
D000081410:Germinal Center Kinases; D016328:NF-kappa B; D010446:Peptide Fragments; D047992:TNF Receptor-Associated Factor 2; D014409:Tumor Necrosis Factor-alpha; D025801:Ubiquitin; C489230:ubiquitin 50-59, human; D017346:Protein Serine-Threonine Kinases; C000631454:TNIK protein, human