Hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for highly rapid and sensitive analysis of underivatized amino acids in functional foods.
This work presented a new analytical methodology based on hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry in multiple-reaction monitoring mode for analysis of 24 underivatized free amino acids (FAAs) in functional foods. The proposed method was first reported and validated by assessing the matrix effects, linearity, limit of detections and limit of quantifications, precision, repeatability, stability and recovery of all target compounds, and it was used to determine the nutritional substances of FAAs in ginkgo seeds and further elucidate the nutritional value of this functional food. The result showed that ginkgo seed turned out to be a good source of FAAs with high levels of several essential FAAs and to have a good nutritional value. Furthermore, the principal component analysis was performed to classify the ginkgo seed samples on the basis of 24 FAAs. As a result, the samples could be mainly clustered into three groups, which were similar to areas classification. Overall, the presented method would be useful for the investigation of amino acids in edible plants and agricultural products.
Jiangsu Key Laboratory for High Technology of TCM formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Guisheng
G
Zhou
Hanqing
H
Pang
Yuping
Y
Tang
Xin
X
Yao
Xuan
X
Mo
Shaoqing
S
Zhu
Sheng
S
Guo
Dawei
D
Qian
Yefei
Y
Qian
Shulan
S
Su
Li
L
Zhang
Chun
C
Jin
Yong
Y
Qin
Jin-ao
JA
Duan
D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D020441:Ginkgo biloba / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D000596:Amino Acids