Ultrastructural changes during spermatogenesis, biochemical and hormonal evidences of testicular toxicity caused by TBT in freshwater prawn Macrobrachium rosenbergii (De Man, 1879).
The present investigation documents the impact of tributyltin (TBT) on the ultrastructural variation of spermatogenesis in freshwater prawn Macrobrachium rosenbergii. The environmentally realistic concentration of TBT can cause damages to the endocrine and reproductive physiology of crustaceans. In this context, three concentrations viz. 10, 100, and 1000 ng/L were selected and exposed to prawns for 90 days. The TBT exposed prawn exhibited decrease the reproductive activity as evidenced by sperm count and sperm length compared to control. Histopathological results revealed the retarded testicular development, abnormal structure of seminiferous tubule, decrease in the concentration of spermatozoa, diminution of seminiferous tubule membrane, abundance of spermatocytes and vacuolation in testis of treated prawns. Ultrastructural study also confirmed the impairment of spermatogenesis in treated prawns. Furthermore, radioimmunoassay (RIA) clearly documented the reduction of testosterone level in TBT exposed groups. Thus, TBT substantially reduced the level of male sex hormone as well as biochemical constituents which ultimately led to impairment of spermatogenesis in the freshwater male prawn M. rosenbergii.
Department of Environmental Biotechnology, Bharathidasan University, Trichy 620 024, Tamil Nadu, India.
Peranandam
P
Revathi
Palanisamy
P
Iyapparaj
Lourduraj Arockia
LA
Vasanthi
Natesan
N
Munuswamy
Muthukalingan
M
Krishnan
D000818:Animals; D008297:Male; D033541:Palaemonidae / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013737:Testis / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin
Macrobrachium rosenbergii; male reproductive activity; spermatogenesis; testosterone; transmission electron microscopy; tributyltin