High quality de novo sequencing and assembly of the Saccharomyces arboricolus genome.
BACKGROUND
Comparative genomics is a formidable tool to identify functional elements throughout a genome. In the past ten years, studies in the budding yeast Saccharomyces cerevisiae and a set of closely related species have been instrumental in showing the benefit of analyzing patterns of sequence conservation. Increasing the number of closely related genome sequences makes the comparative genomics approach more powerful and accurate.


RESULTS
Here, we report the genome sequence and analysis of Saccharomyces arboricolus, a yeast species recently isolated in China, that is closely related to S. cerevisiae. We obtained high quality de novo sequence and assemblies using a combination of next generation sequencing technologies, established the phylogenetic position of this species and considered its phenotypic profile under multiple environmental conditions in the light of its gene content and phylogeny.


CONCLUSIONS
We suggest that the genome of S. arboricolus will be useful in future comparative genomics analysis of the Saccharomyces sensu stricto yeasts.
Institute of Research on Cancer and Ageing of Nice (IRCAN), CNRS UMR 7284 - INSERM U1081, Université de Nice Sophia Antipolis, 06107, NICE Cedex 2, France. Gianni.Liti@unice.fr
Gianni
G
Liti
Alex N
AN
Nguyen Ba
Martin
M
Blythe
Carolin A
CA
Müller
Anders
A
Bergström
Francisco A
FA
Cubillos
Felix
F
Dafhnis-Calas
Shima
S
Khoshraftar
Sunir
S
Malla
Neel
N
Mehta
Cheuk C
CC
Siow
Jonas
J
Warringer
Alan M
AM
Moses
Edward J
EJ
Louis
Conrad A
CA
Nieduszynski
D005800:Genes, Fungal / Q000235:genetics; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D020407:Internet; D058977:Molecular Sequence Annotation; D010641:Phenotype; D010802:Phylogeny; D012440:Saccharomyces / Q000235:genetics*; D013045:Species Specificity