Acute aerocystitis in Piaractus mesopotamicus: participation of eicosanoids and pro-inflammatory cytokines.
A total of 360 pacus (Piaractus mesopotamicus) were used to study vascular permeability (VP) and inflammatory cell component (CC) in induced aerocystitis in P. mesopotamicus through inoculation of inactivated Aeromonas hydrophila, and the effect of steroidal and nonsteroidal anti-inflammatory drugs. It was observed that after inoculation of A. hydrophila, the maximum VP occurred 180 min post-stimulus (MPS). Pretreatment with anti-inflammatory drugs inhibited VP, and the inhibitory effect of dexamethasone was seen earlier than the effects caused by meloxicam and indomethacin. Inoculation of the bacterium caused a gradual increase in the accumulation of cells, which reached a maximum 24 h post-stimulus (HPS). Pretreatment with dexamethasone, indomethacin and meloxicam reduced the accumulation of lymphocytes, thrombocytes, granulocytes and macrophages. There was no significant difference between the different doses of the drugs tested. The results suggest that eicosanoids and pro-inflammatory cytokines participate in chemical mediation in acute inflammation in pacus.
Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Via Prof. Paulo Donato Castellane, km 05, Jaboticabal, SP 14884-900, Brazil.
Gustavo da Silva
Gda S
Claudiano
Thalita R
TR
Petrillo
Wilson G
WG
Manrique
Marcello P
MP
Castro
Bruna A
BA
Loureiro
Paulo F
PF
Marcusso
Marco A A
MA
Belo
Julieta R E
JR
Moraes
Flávio Ruas
FR
de Moraes
D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D002199:Capillary Permeability; D022082:Cellular Structures; D060126:Characidae / Q000276:immunology* / Q000382:microbiology*; D016207:Cytokines / Q000378:metabolism; D003907:Dexamethasone / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D015777:Eicosanoids / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D007213:Indomethacin / Q000008:administration & dosage; D000077239:Meloxicam; D013843:Thiazines / Q000008:administration & dosage; D013844:Thiazoles / Q000008:administration & dosage; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000008:administration & dosage
D000893:Anti-Inflammatory Agents; D001428:Bacterial Vaccines; D016207:Cytokines; D015777:Eicosanoids; D013843:Thiazines; D013844:Thiazoles; D015164:Vaccines, Inactivated; D003907:Dexamethasone; D000077239:Meloxicam; D007213:Indomethacin