Unveiling LOX-1 receptor interplay with nanotopography: mechanotransduction and atherosclerosis onset.
Lectin-like ox-LDL receptors (LOX-1) play a crucial role in the ox-LDL-induced pathological transformation of vessel-wall components, a crucial early step in atherogenesis. LOX-1 dynamics is quantitatively investigated in human endothelial cells (HUVECs) exposed to environmental nanotopographies. We demonstrate distinct nanotopography-induced cell phenotypes, characterized by different morphology, LOX-1 diffusivity and oligomerization state: HUVECs on flat surfaces exhibit the behavior found in pro-atherogenic conditions, while growth on nanogratings can interfere with LOX-1 dynamics and lead to a behavior characteristic of normal, non-pathological conditions.
NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Piazza San Silvestro 12 , 56127 Pisa, Italy.
Carmine
C
Di Rienzo
Emanuela
E
Jacchetti
Francesco
F
Cardarelli
Ranieri
R
Bizzarri
Fabio
F
Beltram
Marco
M
Cecchini
D050197:Atherosclerosis / Q000378:metabolism / Q000473:pathology*; D048430:Cell Shape; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D040542:Mechanotransduction, Cellular; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology; D017382:Reactive Oxygen Species / Q000378:metabolism; D051127:Scavenger Receptors, Class E / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
C495137:OLR1 protein, human; D017382:Reactive Oxygen Species; D051127:Scavenger Receptors, Class E