Cyr61, a matricellular protein, is needed for dendritic arborization of hippocampal neurons.
The shape of the dendritic arbor is one of the criteria of neuron classification and reflects functional specialization of particular classes of neurons. The development of a proper dendritic branching pattern strongly relies on interactions between the extracellular environment and intracellular processes responsible for dendrite growth and stability. We previously showed that mammalian target of rapamycin (mTOR) kinase is crucial for this process. In this work, we performed a screen for modifiers of dendritic growth in hippocampal neurons, the expression of which is potentially regulated by mTOR. As a result, we identified Cyr61, an angiogenic factor with unknown neuronal function, as a novel regulator of dendritic growth, which controls dendritic growth in a β1-integrin-dependent manner.
Laboratory of Molecular and Cellular Neurobiology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland.
Anna R
AR
Malik
Laboratory of Molecular and Cellular Neurobiology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland.
Malgorzata
M
Urbanska
Laboratory of Molecular and Cellular Neurobiology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland.
Agata
A
Gozdz
Laboratory of Molecular and Cellular Neurobiology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland.
Lukasz J
LJ
Swiech
Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland.
Andrzej
A
Nagalski
Laboratory of Molecular and Cellular Neurobiology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland.
Malgorzata
M
Perycz
Laboratory of Molecular and Cellular Neurobiology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland.
Magdalena
M
Blazejczyk
Laboratory of Molecular and Cellular Neurobiology, 4 Ks. Trojdena St., 02-109 Warsaw, Poland. Electronic address: jaworski@iimcb.gov.pl.
Jacek
J
Jaworski
D000818:Animals; D048430:Cell Shape; D002478:Cells, Cultured; D055515:Cysteine-Rich Protein 61 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003712:Dendrites / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D017781:Genes, Immediate-Early; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D007328:Insulin / Q000502:physiology; D019012:Integrin beta1 / Q000378:metabolism / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D019893:Ribosomal Protein S6 Kinases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism / Q000502:physiology
D055515:Cysteine-Rich Protein 61; D048049:Extracellular Signal-Regulated MAP Kinases; D007328:Insulin; D019012:Integrin beta1; D019869:Phosphatidylinositol 3-Kinases; D034741:RNA, Small Interfering; D019893:Ribosomal Protein S6 Kinases; D058570:TOR Serine-Threonine Kinases; D018631:ras Proteins; C501849:CCN1 protein, rat; C546845:mTOR protein, rat; C526901:Rps6kb1 protein, rat