Influence of track directions on the biological consequences in cells irradiated with high LET heavy ions.
OBJECTIVE
The impact of the damage distribution to cellular survival and chromosomal aberrations following high Linear Energy Transfer (LET) radiation was investigated.


METHODS
High LET iron-ions (500 MeV/n, LET 200 keV/μm) were delivered to G1-phase synchronized Chinese Hamster Ovary (CHO) cells located at a vertical or horizontal angle relative to the ion beam in order to give same dose but different fluence and damage distribution.


RESULTS
Horizontal irradiation produced DNA double-strand break (DSB) along each ion track represented as clustered lines, and vertical irradiation produced a greater fluence. The initial damages measured by premature chromosome condensation were equal per dose in both irradiation types. Horizontal irradiation proved to be less effective in cell killing than vertical at doses of more than 3 Gy. Vertical irradiation produced a higher number of metaphase chromosomal aberrations compared to horizontally irradiated samples. In particular, formation of exchange-type aberrations was the same, but that of deletion-type aberrations were significantly higher after vertical irradiation than horizontal irradiation.


CONCLUSIONS
Therefore, we concluded that high fluence per dose is more effective than low fluence per dose to produce radiation-induced chromosomal aberrations and to kill exposed cells following high LET heavy-ion exposure.
Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Inage, Chiba, Japan.
Yoshihiro
Y
Fujii
Charles R
CR
Yurkon
Junko
J
Maeda
Stefan C
SC
Genet
Ryuichi
R
Okayasu
Hisashi
H
Kitamura
Akira
A
Fujimori
Takamitsu A
TA
Kato
D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000528:radiation effects*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000502:physiology*; D004307:Dose-Response Relationship, Radiation; D020450:Heavy Ions; D018499:Linear Energy Transfer / Q000502:physiology*; D011829:Radiation Dosage; D012542:Scattering, Radiation