Expression and localization of COMMD1 proteins in human placentas from women with preeclampsia.
OBJECTIVE
Recently, COMMD1 has been identified as a novel interactor and regulator of hypoxia-inducible factor-1 and nuclear factor kappa B transcriptional activity. The goal of this study was to determine the difference of COMMD1 expression in the placentas of women with normal and preeclamptic (PE) pregnancies.


METHODS
Immnoperoxidase and immunofluorescent staining for COMMD1 was performed on nine normal and nine severe PE placental tissues, and COMMD1 mRNA expression was quantified by quantitative reverse transcription polymerase chain reaction.


RESULTS
The expression of mRNA of COMMD1 was significantly higher in the study group than in the control group. The immunoreactivity was higher especially in the syncytiotrophoblast of PE placentas than in the control group.


CONCLUSIONS
This study demonstrated increased placental COMMD1 expression in women with severe preeclampsia compared to that found in women with normal pregnancies, and this finding might contribute to a better understanding of the pathophysiology of preeclampsia.
Department of Obstetrics and Gynecology, Konkuk University School of Medicine, 120-1 Neungdong-ro, Gwangjin-gu, Seoul 143-729, Korea.
Han-Sung
HS
Kwon
Seung-Hwa
SH
Park
Han-Sung
HS
Hwang
In-Sook
IS
Sohn
Soo-Nyung
SN
Kim
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000328:Adult; D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; C480519:COMMD1 protein, human; D012333:RNA, Messenger