[HILIC-eLSD determination of five oligosaccharides contained in Morinda officinalis].
OBJECTIVE
To determine determinate five oligosaccharides, namely sucrose, 1-kestose, nystose, 1F-fructofurano-syinystose, bajijiasu contained in Morinda officinalis with an HILIC-ELSDI) method.


METHODS
Waters XBridge Amide (4.6 mm x 150 mm, 3.5 microm) hilic column was adopted for gradient elution, with acetonitrile (A) and 0.2% triethylamine (B) as the mobile phase. The column temperature was set at 40 degrees C, with the flow rate of 0.8 mL x min. Waters 2424 evaporative light scattering detector (ESLD) was used as detector, with the gas flow of 275.79 kPa and drift tube temperature of 90 degrees C.


RESULTS
The detection range for the five oligosaccharides were 2.128-21.28 microg for sucrose (r = 0.999 3), 1.864-18.64 microg for 1-kestose (r = 0.999 6), 1.92-19.2 microg for nystose (r = 0.999 8), 1.912-19. 12 microg for 1F-fructofuranosyinystose (r = 0.999 5), 2.368-23.68 microg for bajijiasu (r = 0.999 4), respectively. The recovery of the five oligosaccharides ranged between 92.81%-102.8% (n = 6).


CONCLUSIONS
The method is so simple, accurate and highly reproducible that it can be used as an analytical method for effective evaluation of the quality of M. officinalis herbs.
(School of Chinese Herbal Medicine, Guangzhou University of traditional Chinese medicine, Guangzhou 510006, China. 59543177@qq.com
Shao-Dlong
SD
Deng
Feng-Xia
FX
Xiao
Li
L
Lin
Peng
P
Zhang
Jing-Ran
JR
Lin
Sheng-Bin
SB
Zhang
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D032066:Morinda / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis*; D012542:Scattering, Radiation
D004365:Drugs, Chinese Herbal; D009844:Oligosaccharides