DNA microarray analysis of hepatic gene expression in mice exposed to cadmium for 30 days.
Although cadmium causes hepatotoxicity, its molecular mechanism is unclear. In the present study, transcriptional responses in the liver of C57BL/6J mice given 50 ppm cadmium as a drinking water for 30 days were evaluated with DNA microarray. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were not elevated following the administration of cadmium. Cadmium increased the expressions of 2 genes and reduced those of 15 genes in the liver of mice before the leading to hepatotoxicity.
Laboratory of Chemical Toxicology and Environmental Health, Showa Pharmaceutical University, Tokyo, Japan.
Maki
M
Tokumoto
Tomoaki
T
Ohtsu
Shunji
S
Imai
Akiko
A
Honda
Hisamitsu
H
Nagase
Masahiko
M
Satoh
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002104:Cadmium / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis
D004785:Environmental Pollutants; D002104:Cadmium; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase