Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle.
Mesenchymal stem cells (MSC) generated from human umbilical cord (UC-MSC) and placenta (PLC-MSC) were assessed and compared for their immunomodulatory function on T cells proliferation by analysis of the cell cycle. Mitogen stimulated or resting T cells were co-cultured in the presence or absence of MSC. T-cell proliferation was assessed by tritiated thymidine ((3) H-TdR) assay and the mechanism of inhibition was examined bycell cycle and apoptosis assay. Both UC-MSC and PLC-MSC profoundly inhibited the proliferation of T-cell, mainly via cell-to-cell contact. MSC-mediated anti-proliferation does not lead to apoptosis,but prevented T cells from entering S phase and they therefore accumulated in the G(0) /G(1)  phases. The anti-proliferative activity of MSC was related to this cell cycle arrest of T-cell. UC-MSC produced a greater inhibition than PLC-MSC in all measured parameters.
Immunology Laboratory, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Shalini
S
Vellasamy
Pratheep
P
Sandrasaigaran
Sharmili
S
Vidyadaran
Maha
M
Abdullah
Elizabeth
E
George
Rajesh
R
Ramasamy
D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D016192:Resting Phase, Cell Cycle; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D014470:Umbilical Cord / Q000166:cytology