Differential effects of lipoprotein apheresis by lipidfiltration or dextran sulfate adsorption on lipidomic profile.
OBJECTIVE
Acute modification of plasma lipidomic profile was assessed by top-down shotgun profiling on a LTQ Orbitrap hybrid mass spectrometer in 14 patients treated with two different apheresis techniques: plasma lipidfiltration (LF) and whole blood dextran sulfate adsorption (DSA).


RESULTS
Patients treated with DSA revealed a significantly more pronounced reduction of LDL-cholesterol (LDL-C), a diminished decrease of HDL-cholesterol (HDL-C) and triglycerides (TG), and a similar reduction in lipoprotein (a) (Lp(a)) level. Against the overall tendency of reduction of lipid metabolites of all lipid classes in post-apheresis plasma, independent of apheresis technology applied, a highly significant increase of phosphatidylethanolamines (PE) in response to DSA was observed.


CONCLUSIONS
These data indicate that DSA technology may be associated with an activation or damage of blood cells at contact surface which subsequently leads to a massive liberation of cellular and membrane PE's. Pathophysiological consequences, especially with respect to coagulation system and oxidative stress, have to be further elucidated.
Department of Internal Medicine III, University Hospital Carl Gustav Carus Dresden, Germany. Juergen.Graessler@uniklinikum-dresden.de
J
J
Gräßler
S
S
Kopprasch
J
J
Passauer
S
S
Fischer
K
K
Schuhmann
S
S
Bergmann
G
G
Siegert
A
A
Shevchenko
S R
SR
Bornstein
U
U
Julius
D000327:Adsorption; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D001781:Blood Component Removal / Q000009:adverse effects / Q000379:methods*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D016264:Dextran Sulfate / Q000009:adverse effects / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000175:diagnosis / Q000628:therapy*; D017270:Lipoprotein(a) / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D010714:Phosphatidylethanolamines / Q000097:blood; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood
D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D017270:Lipoprotein(a); D008074:Lipoproteins; D010714:Phosphatidylethanolamines; D014280:Triglycerides; C483858:phosphatidylethanolamine; D016264:Dextran Sulfate