In vivo biological responses to silk proteins functionalized with bone sialoprotein.
Recombinant 6mer + BSP protein, combining six repeats of the consensus sequence for Nephila clavipes dragline (6mer) and bone sialoprotein sequence (BSP), shows good support for cell viability and induces the nucleation of hydroxyapatite and tricalcium phosphate during osteoblast in vitro culture. The present study is conducted to characterize this bioengineered protein-based biomaterial further for in vivo behavior related to biocompatibility. 6mer + BSP protein films are implanted in subcutaneous pouches in the back of mice and responses are evaluated by flow cytometry and histology. The results show no major differences between the inflammatory responses induced by 6mer + BSP films and the responses observed for the controls. Thus, this new chimeric protein could represent an alternative for bone regeneration applications.
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Indústrial da Gandra, 4806-909 Caldas das Taipas, Guimarães, Portugal.
Sílvia
S
Gomes
Jabier
J
Gallego-Llamas
Isabel B
IB
Leonor
João F
JF
Mano
Rui L
RL
Reis
David L
DL
Kaplan
D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D057005:Bioengineering / Q000379:methods*; D001861:Bone Regeneration / Q000502:physiology*; D002130:Calcium Phosphates / Q000378:metabolism; D018929:Cell Culture Techniques; D017886:Durapatite / Q000378:metabolism; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D006652:Histological Techniques; D058574:Integrin-Binding Sialoprotein / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology*; D047011:Silk / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D058574:Integrin-Binding Sialoprotein; D047011:Silk; D017886:Durapatite; C018392:tricalcium phosphate