High-fat feeding, but not strenuous exercise, increases blood oxidative stress in trained men.
Two prevalent origins of oxidative stress in Western society are the ingestion of high-fat meals and the performance of strenuous exercise. The purpose of this investigation was to compare the magnitude of increase in blood oxidative stress following acute feeding and acute exercise. Twelve exercise-trained men consumed a high-fat meal or performed 1 of 3 exercise bouts (steady-state aerobic; high-intensity, moderate-duration interval sprints; maximal intensity, short-duration interval sprints) in a random order, crossover design. Blood was collected before and at times following feeding and exercise. Samples were analyzed for trigylcerides, malondialdehyde (MDA), hydrogen peroxide (H(2)O(2)), advanced oxidation protein products (AOPP), nitrate/nitrite (NOx), trolox-equivalent antioxidant capacity (TEAC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). A significant condition effect was noted for MDA (p = 0.01), H(2)O(2) (p < 0.0001), and AOPP (p = 0.0006), with values highest for the meal condition. An increase of 88%, 247%, and 96% was noted from pre- to post-feeding for MDA, H(2)O(2), and AOPP, respectively. A condition effect was also noted for TEAC (p = 0.04) and CAT (p = 0.05), with values lowest for the meal condition (TEAC) and the meal and aerobic exercise condition (CAT). NOx, SOD, and GPx were relatively unaffected by feeding and exercise, while MDA, H(2)O(2), and AOPP experienced little change from pre- to postexercise (p > 0.05). These results illustrate that the magnitude of blood oxidative stress following a high-fat meal is significantly greater than that elicited by either aerobic or anaerobic exercise in a sample of exercise-trained men.
Cardiorespiratory/Metabolic Laboratory, The University of Memphis, Memphis, TN 38152, USA.
Cameron G
CG
McCarthy
Tyler M
TM
Farney
Robert E
RE
Canale
Michael E
ME
Dessoulavy
Richard J
RJ
Bloomer
D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D002839:Chromans / Q000097:blood; D018592:Cross-Over Studies; D059305:Diet, High-Fat / Q000379:methods*; D004041:Dietary Fats / Q000008:administration & dosage / Q000097:blood*; D015444:Exercise / Q000502:physiology*; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D019518:Postprandial Period; D013482:Superoxide Dismutase / Q000097:blood; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
D062107:Advanced Oxidation Protein Products; D015415:Biomarkers; D002839:Chromans; D004041:Dietary Fats; D009566:Nitrates; D009573:Nitrites; D014280:Triglycerides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid