The utilization of perforated bioinert chambers to generate an in vivo isolated space for tissue engineering involving chondrocytes, mesenchymal stem cells, and fibroblasts.
Vascular invasion and admixture of the nude mouse cells with seeded cells make it difficult to reapply the regenerated tissues to the restoration of host tissue defects. Therefore, a device that is capable of allowing for autologous or allogenic tissue growth while preventing host tissue invasion will be a valuable tool for in vivo tissue engineering. We have previously fabricated a novel silicon-perforated chamber. The aim of this study was to evaluate whether this chamber, after being implanted subcutaneously in experimental animals, would hinder host tissue ingrowth while providing an environment inside its cavity for in vivo growth of either autologous or allogenic implant cells. We found that the chamber did not induce severe foreign body reaction, and the chambers with perforated pores of 1-3 mm in diameter effectively inhibited the host granulation tissue or vascular invasion for as long as 3 months. In addition, the exudates rich in vascular endothelial growth factor, basic fibroblast growth factor, transforming growth factor-β, insulin-like growth factor-1, and platelet-derived growth factor-BB were steadily generated and collected in the chambers. In vitro cell culture studies revealed that the exudates were able to support the viability and proliferation of rabbit chondrocytes, rat mesenchymal stem cells, and human fibroblasts. The results indicate that this novel chamber could potentially provide an environment favorable for in vivo tissue engineering while effectively preventing host tissue or vascular invasion.
Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, P.R. China.
Shiping
S
Wang
Xiaoyan
X
Hao
Yingjun
Y
Su
Chenggang
C
Yi
Bing
B
Li
Xing
X
Fan
Jiaomiao
J
Pei
Yajuan
Y
Song
Wei
W
Xia
Bei
B
Liu
Shuzhong
S
Guo
D000818:Animals; D019149:Bioreactors; D002232:Carbocyanines / Q000378:metabolism; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D019902:Chondrocytes / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D019919:Prosthesis Implantation; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D040521:Subcutaneous Tissue / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation*
C101089:CM-DiI; D002232:Carbocyanines; D036341:Intercellular Signaling Peptides and Proteins