[Cloning and functional research of Arp2/3-P40/ARPC1 subunit of Sf9 cells].
The baculovirus-induced actin polymerization is mainly associated with the virus nucleocapsid protein P78/83, which is homologous with WASP proteins that can activate Arp2/3 complex and induce the actin polymerization. In order to explore the role of Arp2/3 complex in the baculovirus replication, the P40 subunit of Arp2/3 complex from Sf9 (Spodoptera frugiperda 9) cell line was cloned and characterized. Immunofluorescent microscopy assay indicated that P40 was recruited to the inner-side of nuclear membrane during virus infection, which was in accordance with nuclear F-actin distribution in virus-infected cells as documented in our previous research, suggesting P40 could be used to track Arp2/3 complex subcellular distribution changes during virus infection. In addition, co-immunoprecipitation assay demonstrated that P40 interacted with P78/83 only in virus-infected cells, suggesting that actin polymerization induced by P78/83-Arp2/3 complex during baculovirus infection was regulated by some unidentified virus factors.
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan 430079, China. shanddonghanshili@126.com
Shi-Li
SL
Han
Jing-Fang
JF
Mu
Yong-Li
YL
Zhang
Xin-Wen
XW
Chen
Yun
Y
Wang
Lu-Lin
LL
Li
D051376:Actin-Related Protein 2-3 Complex / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D016415:Sequence Alignment; D061987:Sf9 Cells; D018411:Spodoptera / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism* / Q000821:virology
D051376:Actin-Related Protein 2-3 Complex; D036022:Capsid Proteins; D019476:Insect Proteins