In vitro dermal absorption of di(2-ethylhexyl) adipate (DEHA) in a roll-on deodorant using human skin.
In vitro dermal absorption experiments were conducted using a roll-on deodorant that contains 1.56% di(2-ethylhexyl) adipate (DEHA), a plasticizer widely used in consumer products. Human skin specimens were fitted in Bronaugh flow-through Teflon diffusion cells. The diffusion cells were maintained at 32 °C to reflect the skin temperature. Two amounts (low dose: 5 mg of the product; high dose: 100 mg) were applied, in triplicate, each on four different human skins. DEHA was determined in the receiver solution at 6-h intervals, using headspace solid-phase microextraction gas chromatography-mass spectrometry (GC-MS). After 24 h, the experiment was terminated and masses of DEHA in the skin depot, skin wash, and upper and lower chambers of the diffusion cell were determined. A significant portion of applied DEHA, 28% in the low amount application and 34% in the high one, was found in the skin depot. In comparison, only 0.04% and 0.002% of applied DEHA were found in the receiver solutions for the low and high doses, respectively. Under our experimental conditions, an apparent steady-state flux of low DEHA mass penetrating from skin into the receiver solution was observed with a penetration rate of 2.2 ng/cm(2)/h for both the low and high doses. The average mass recovery was 81% for the low dose application and 56% for the high dose.
Exposure and Biomonitoring Division, Health Canada, Ottawa, Ontario, Canada.
Simon Ningsun
SN
Zhou
Richard P
RP
Moody
Bio
B
Aikawa
Anna
A
Yip
Bing
B
Wang
Jiping
J
Zhu
D000272:Adipates / Q000493:pharmacokinetics*; D000279:Administration, Cutaneous; D003257:Consumer Product Safety; D003836:Deodorants / Q000493:pharmacokinetics*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D066298:In Vitro Techniques; D010968:Plasticizers; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013997:Time Factors
D000272:Adipates; D003836:Deodorants; D010968:Plasticizers; C013966:dioctyl adipate