[Ectopic osteogenesis of SGDs in goat's muscle pouch].
OBJECTIVE
To investigate ectopic osteogenesis of composites SGDs with porous calcium phosphate cement(pCPC) in goat's muscle pouch.


METHODS
SGDs were cultivated with modified tissue culture techniques, then were induced into osteoblasts in the third passage, the osteogenic-induced SGDs were combined with pCPC and transplanted into the goat left dorsal muscle pouch, the pCPC without cells was transplanted into the right dorsal muscle pouch as negative controls. The transplants were harvested at 2-,4-,6-,8- week and prepared for histological examination. The morphologic quantitative analysis was made by SPSS 16.0 software package.


RESULTS
Bone formation was not detected in pCPC without cells by histological examination. 2,4,6,8 weeks after transplantation in SGDs-pCPC group, the percentages of bone formation were (1.24±0.25)%,(1.59±0.23)%,(4.12±0.39)% and (5.68±0.58)%,respectively.There was no significant difference in bone formation at 2 and 4 weeks after transplantation (P>0.05). At 8- week, the percentages of bone formation were higher than that at 6- week in SGDs-pCPC group, and both significantly higher than that at 2- and 4- week(P<0.05).


CONCLUSIONS
SGDs combined with pCPC have the ability of ectopic osteogenesis.
Department of Stomatology, the Sixth People's Hospital, Shanghai Jiao Tong University. Shanghai 200233, China. haoyongming3804@126.com
Yong-ming
YM
Hao
Wei
W
Zhao
Xiu-li
XL
Zhang
Jia-yu
JY
Lu
Yi
Y
Wang
De-rong
DR
Zou
D000818:Animals; D001854:Bone Marrow Cells; D002130:Calcium Phosphates; D002478:Cells, Cultured; D006041:Goats; D010006:Osteoblasts; D010012:Osteogenesis
D002130:Calcium Phosphates; C020243:calcium phosphate