Characterization of multidrug-resistant Proteus mirabilis isolated from chicken carcasses.
This study investigated genotypic and phenotypic features of antimicrobial resistance of Proteus mirabilis isolated from chicken products. Resistance to a broad spectrum of antimicrobial agents was commonly observed in the test isolates: tetracycline (100%), sulfamethoxazole (80%), chloramphenicol (66%), nalidixic acid (66%), ampicillin (60%), streptomycin (56%), ciprofloxacin (52%), kanamycin (46%), gentamicin (38%), ceftriaxone (36%), cefotaxime (34%), ceftiofur (22%), and amoxicillin-clavulanic acid (16%). The β-lactamases TEM-1 and OXA-1, and extended-spectrum β-lactamases CTX-M-9 and CMY-2 were detected in β-lactam-resistant isolates. Single mutations in gyrA and parC were found to be contributing factors for fluoroquinolone resistance. Plasmid-mediated quinolone resistance (PMQR) genes qnrA and qnrD were detected in six fluoroquinolone-resistant isolates and a superintegron element, SXT, was detected in 14 out of 50 isolates. The high-level of antimicrobial resistance of P. mirabilis isolated from food products may pose a potential threat to public health.
Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR.
Marcus Ho Yin
MH
Wong
Hoi Ying
HY
Wan
Sheng
S
Chen
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002645:Chickens / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005506:Food Contamination; D005516:Food Microbiology; D008460:Meat; D008826:Microbial Sensitivity Tests; D011513:Proteus mirabilis / Q000187:drug effects* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; C486443:beta-lactamase CTX-M-19; C034413:beta-lactamase TEM-1; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase