Effects of selenium-enriched probiotics on heat shock protein mRNA levels in piglet under heat stress conditions.
The effects of selenium-enriched probiotics (SP) on tissue selenium (Se) deposition, glutathione peroxidase-1 (GPx1) activity and mRNA level, and heat shock protein (Hsp) mRNA levels of piglets under heat stress conditions were investigated. A total of 48 crossbred ([Landrace × Yorkshire] × Duroc) piglets were randomly divided into 4 groups and fed a basal diet (Con, 0.16 mg Se/kg) or basal diets with added probiotics (P, 0.16 mg Se/kg), sodium selenite (SS, 0.46 mg Se/kg), or SP (0.46 mg Se/kg), respectively, for 42 days. Three piglets were randomly selected from each group for blood sample collection at days 0, 14, 28, and 42 and for liver, kidney, and spleen sample collection at day 42. The results showed that P, SS, and SP could significantly down-regulate the average mRNA levels of Hsp70 (17.3, 23.7, and 40.1%) and Hsp27 (22.4, 24.4, and 44.7%) of the tissues, respectively (P < 0.05), whereas SS and SP could significantly elevate Se concentration, GPx1 activity and mRNA level (P < 0.05). The maximal effects of these parameters were observed in SP. It was concluded that SP is a feasible dietary supplementation of piglets under heat stress conditions during the summer season.
Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University , Nanjing 210095, China.
Fang
F
Gan
Fei
F
Ren
Xingxiang
X
Chen
Chenhui
C
Lv
Cuiling
C
Pan
Gengping
G
Ye
Jun
J
Shi
Xiuli
X
Shi
Hong
H
Zhou
Shituleni Andreas
SA
Shituleni
Kehe
K
Huang
D000821:Animal Feed; D000818:Animals; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000378:metabolism; D019936:Probiotics / Q000378:metabolism*; D011897:Random Allocation; D012643:Selenium / Q000032:analysis / Q000378:metabolism*; D013552:Swine / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D000094765:Glutathione Peroxidase GPX1
D006360:Heat-Shock Proteins; D005979:Glutathione Peroxidase; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1