Cellulose membranes are more effective in holding back vital proteins and exhibit less interaction with plasma proteins during hemodialysis.
The vast majority of patients with end-stage renal disease are treated with intermittent hemodialysis as a form of renal replacement therapy. To investigate the impact of hemodialysis membrane material on vital protein removal, dialysates from 26 well-characterized hemodialysis patients were collected 5 min after beginning, during 5h of treatment, as well as 5 min before ending of the dialysis sessions. Dialysis sessions were performed using either modified cellulose (n=12) (low-flux and high flux) or synthetic Polyflux (n=14) (low-flux and high-flux) dialyzer. Protein removal during hemodialysis was quantified and the dialysate proteome patterns were analyzed by 2-DE, MS and Western blot. There was a clear correlation between the type of membrane material and the amount of protein removed. Synthetic Polyflux membranes exhibit strong interaction with plasma proteins resulting in a significantly higher protein loss compared to modified cellulosic membrane. Moreover, the proteomics analysis showed that the removed proteins represented different molecular weight range and different functional groups: transport proteins, protease inhibitors, proteins with role in immune response and regulations, constructive proteins and as a part of HLA immune complex. The effect of this protein removal on hemodialysis treatment outcome should be investigated in further studies.
Department of Nephrology and Rheumatology, Georg-August University Goettingen, Goettingen, Germany.
Ivana
I
Pešić
Gerhard A
GA
Müller
Cosima
C
Baumann
Gry H
GH
Dihazi
Michael J
MJ
Koziolek
Marwa
M
Eltoweissy
Carsten
C
Bramlage
Abdul R
AR
Asif
Hassan
H
Dihazi
D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D002482:Cellulose; D015314:Dialysis Solutions / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D006435:Renal Dialysis; D016896:Treatment Outcome; D001613:beta 2-Microglobulin / Q000097:blood
D001798:Blood Proteins; D015314:Dialysis Solutions; D008567:Membranes, Artificial; D001613:beta 2-Microglobulin; D002482:Cellulose