Microparticles containing propolis and metronidazole: in vitro characterization, release study and antimicrobial activity against periodontal pathogens.
Ethylcellulose microparticles containing metronidazole and propolis extractive solution were prepared and evaluated in vitro against periodontal pathogens. Scanning electron microscopy, particle size analysis, drug entrapment efficiency and drug release of microparticles were determined. The antimicrobial activity of microparticles was evaluated against microorganisms of periodontal importance (Enterococcus faecalis, Streptococcus pyogenes, Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli). It was obtained particles with regular morphology, mean diameter of 1.23 µm, and entrapment efficiency for propolis and metronidazole were 91.41% and 22.23%, respectively. In vitro release studies of propolis and metronidazole from microparticles showed prolonged drug release and controlled by Fickian diffusion. Both propolis and metronidazole displayed activity against the tested strains. Moreover, the results showed that the strains of E. faecalis, S. pyogenes and S. mutans were more susceptible to the propolis and E. faecalis to the metronidazole. It was also observed that the amount of metronidazole to inhibit the microorganism strains in the physical mixture with propolis was smaller than in the metronidazole alone, suggesting potentiation effect between propolis and metronidazole. These microparticles would be useful for developing intermediary or eventual dosage form to be administered into the periodontal pocket more easily and safely.
Department of Pharmacy .
Sabrina Barbosa
SB
de Souza Ferreira
Bruno Rafael
BR
de Assis Dias
Clarissa Silva
CS
Obregón
Carla Carolina
CC
Gomes
Raphaela Regina
RR
de Araújo Pereira
Janine Silva
JS
Ribeiro Godoy
Terezinha Inez
TI
Estivalet Svidzinski
Marcos Luciano
ML
Bruschi
D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000188:drug therapy; D002482:Cellulose / Q000031:analogs & derivatives*; D004337:Drug Carriers / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008795:Metronidazole / Q000008:administration & dosage* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011429:Propolis / Q000008:administration & dosage* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects
D000890:Anti-Infective Agents; D004337:Drug Carriers; D008795:Metronidazole; D002482:Cellulose; D011429:Propolis