title: Essential Staphylococcus aureus toxin export system.
abstract: Widespread antibiotic resistance among important bacterial pathogens such as Staphylococcus aureus calls for alternative routes of drug development. Interfering with crucial virulence determinants is considered a promising new approach to control bacterial infection. Phenol-soluble modulins (PSMs) are peptide toxins with multiple key roles in pathogenesis and have a major impact on the ability of highly virulent S. aureus to cause disease. However, targeting PSMs for therapeutic intervention is hampered by their multitude and diversity. Here we report that an ATP-binding cassette transporter with previously unknown function is responsible for the export of all PSMs, thus representing a single target for complete obstruction of PSM production. The transporter had a strong effect on virulence phenotypes, such as neutrophil lysis, and the extent of its effect on the development of S. aureus infection was similar to that of the sum of all PSMs. Notably, the transporter was essential for bacterial growth. Furthermore, it contributed to producer immunity toward secreted PSMs and defense against PSM-mediated bacterial interference. Our study reveals a noncanonical, dedicated secretion mechanism for an important class of toxins and identifies this mechanism as a comprehensive potential target for the development of drugs to efficiently inhibit the growth and virulence of pathogenic staphylococci.
authors: Pathogen Molecular Genetics Section, Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Som S
authors: SS
authors: Chatterjee
authors: Hwang-Soo
authors: HS
authors: Joo
authors: Anthony C
authors: AC
authors: Duong
authors: Thomas D
authors: TD
authors: Dieringer
authors: Vee Y
authors: VY
authors: Tan
authors: Yan
authors: Y
authors: Song
authors: Elizabeth R
authors: ER
authors: Fischer
authors: Gordon Y C
authors: GY
authors: Cheung
authors: Min
authors: M
authors: Li
authors: Michael
authors: M
authors: Otto
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D010641:Phenotype; D013207:Staphylococcal Skin Infections / Q000382:microbiology* / Q000473:pathology; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001427:Bacterial Toxins; C025971:staphylococcal delta toxin
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