title: Long term cryopreservation in 5% DMSO maintains unchanged CD34(+) cells viability and allows satisfactory hematological engraftment after peripheral blood stem cell transplantation.
abstract: Peripheral blood stem cell cryopreservation is associated with cell damage and decreased viability. We evaluated the impact of up to 10 years of cryopreservation (5% DMSO) on viability of CD34(+) cells utilizing graft samples of consecutive patients (2002-2012) with different malignancies who underwent stem cell collection and transplantation. Viability of CD34(+) cells from oncohaematological patients measured after 5 weeks (97·2 ± 0·6%) or after 9-10 years of cryopreservation (95·9 ± 0·5%) was unaffected. Haemoglobin, granulocyte and platelet recovery after transplantation of long-term cryopreserved grafts occurred within 8-13 days. CD34(+) stem cells can be safely stored up to 9-10 years, without affecting cell viability and clinical effectiveness.
authors: Dipartimento dei Laboratori Diagnostici e per le Terapie Cellulari, Unità di Raccolta e Manipolazione di Cellule Staminali, Centro di Riferimento Oncologico - IRCCS, Aviano, Italy.
authors: L
authors: L
authors: Abbruzzese
authors: F
authors: F
authors: Agostini
authors: C
authors: C
authors: Durante
authors: R T
authors: RT
authors: Toffola
authors: M
authors: M
authors: Rupolo
authors: F M
authors: FM
authors: Rossi
authors: A
authors: A
authors: Lleshi
authors: S
authors: S
authors: Zanolin
authors: M
authors: M
authors: Michieli
authors: M
authors: M
authors: Mazzucato
mesh_terms: D064591:Allografts; D002470:Cell Survival; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D006085:Graft Survival; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000628:therapy*; D036102:Peripheral Blood Stem Cell Transplantation; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide