title: Differential interaction of peptides derived from C-terminal domain of human apolipoprotein E with platelet activating factor analogs.
abstract: Apolipoprotein-derived peptides are promising candidates for the treatment of various inflammatory conditions and the main mechanism proposed for the protective action of these peptides includes binding to pro-inflammatory lipid mediators with high affinity and facilitating their sequestration/metabolism/clearance in the body. Molecules that act as pro-inflammatory lipid mediators differ considerably in their molecular structures, chemical compositions and physicochemical properties. Importance of the properties of pro-inflammatory lipid mediators on the biological activity of apolipoprotein-derived peptides has not been studied in detail. In this study, we characterized the physicochemical properties of aggregates containing lyso-PAF, acetyl-PAF and butanoyl-PAF, three closely related pro-inflammatory lipid mediators, and studied their interaction with peptides derived from the C-terminal domains of human apolipoprotein E. These PAF-analogs differ only in the chemical composition of the functional groups they carry at the sn-2 positions. Our results show that physicochemical properties of aggregates containing lyso-PAF, acetyl-PAF and butanoyl-PAF differ considerably and affect their apolipoprotein-derived peptides-binding capacity.
authors: Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Mohali 160062, Punjab, India.
authors: Sunil A
authors: SA
authors: Nankar
authors: Priyanka
authors: P
authors: Bajaj
authors: Revuri
authors: R
authors: Sravanthi
authors: Abhay H
authors: AH
authors: Pande
mesh_terms: D000595:Amino Acid Sequence; D001057:Apolipoproteins E / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001057:Apolipoproteins E; D010455:Peptides; D010972:Platelet Activating Factor