title: miR-370 is stage-specifically expressed during mouse embryonic development and regulates Dnmt3a.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that participate in a large variety of biological processes. In this paper, the spatiotemporal expression pattern of miR-370 was characterized during mouse embryonic development, and was found to be stage- and tissue-specifically expressed. In addition, through luciferase reporter assays and western blot analyses, DNA methyltransferase 3A (Dnmt3a) was identified as a directly regulated target of miR-370. Altogether, our results indicate that miR-370 may play important roles in the morphogenesis of diverse organs, especially brain and adrenal glands, by mediating Dnmt3a expression during mouse development.
authors: School of Life Science and Biotechnology, Harbin Institute of Technology, No 92 West Da-zhi Street, Harbin, Heilongjian 150001, China.
authors: Liu
authors: L
authors: Qi
authors: He
authors: H
authors: Hongjuan
authors: Gu
authors: G
authors: Ning
authors: Han
authors: H
authors: Zhengbin
authors: Xing
authors: X
authors: Yanjiang
authors: Zeng
authors: Z
authors: Tiebo
authors: Huang
authors: H
authors: Zhijun
authors: Wu
authors: W
authors: Qiong
mesh_terms: D000311:Adrenal Glands / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism; D000090205:DNA Methyltransferase 3A; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D008156:Luciferases; D051379:Mice; D008813:Mice, Inbred ICR; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D015398:Signal Transduction; D013997:Time Factors
chemical_list: C000715535:Dnmt3a protein, mouse; C551449:MIRN370 microRNA, mouse; D035683:MicroRNAs; D008156:Luciferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A