title: Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams.
abstract: UVB radiation suppresses photosynthesis and protein biosynthesis in plants, which in turn decreases growth and productivity. Here, an ultraviolet-B (UVB)-tolerant rice mutant, utr319 (UV Tolerant Rice 319), was isolated from a mutagenized population derived from 2500 M1 seeds (of the UVB-resistant cultivar 'Sasanishiki') that were exposed to carbon ions. The utr319 mutant was more tolerant to UVB than the wild type. Neither the levels of UVB-induced cyclobutane pyrimidine dimers (CPDs) or (6-4) pyrimidine-pyrimidone photodimers [(6-4) photoproducts], nor the repair of CPDs or (6-4) photoproducts, was altered in the utr319 mutant. Thus, the utr319 mutant may be impaired in the production of a previously unidentified factor that confers UVB tolerance. To identify the mutated region in the utr319 mutant, microarray-based comparative genomic hybridization analysis was performed. Two adjacent genes on chromosome 7, Os07g0264900 and Os07g0265100, were predicted to represent the mutant allele. Sequence analysis of the chromosome region in utr319 revealed a deletion of 45 419 bp. RNAi analysis indicated that Os07g0265100 is most likely the mutated gene. Database analysis indicated that the Os07g0265100 gene, UTR319, encodes a putative protein with unknown characteristics or function. In addition, the homologs of UTR319 are conserved only among land plants. Therefore, utr319 is a novel UVB-tolerant rice mutant and UTR319 may be crucial for the determination of UVB sensitivity in rice, although the function of UTR319 has not yet been determined.
authors: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577, Japan.
authors: Nao
authors: N
authors: Takano
authors: Yuko
authors: Y
authors: Takahashi
authors: Mitsuru
authors: M
authors: Yamamoto
authors: Mika
authors: M
authors: Teranishi
authors: Hiroko
authors: H
authors: Yamaguchi
authors: Ayako N
authors: AN
authors: Sakamoto
authors: Yoshihiro
authors: Y
authors: Hase
authors: Hiroko
authors: H
authors: Fujisawa
authors: Jianzhong
authors: J
authors: Wu
authors: Takashi
authors: T
authors: Matsumoto
authors: Seiichi
authors: S
authors: Toki
authors: Jun
authors: J
authors: Hidema
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D002244:Carbon / Q000737:chemistry*; D002875:Chromosomes / Q000528:radiation effects; D022202:Chromosomes, Artificial, Bacterial; D055028:Comparative Genomic Hybridization; D004260:DNA Repair; D015723:Gene Library; D020450:Heavy Ions; D008969:Molecular Sequence Data; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000528:radiation effects*; D010641:Phenotype; D010788:Photosynthesis / Q000528:radiation effects; D030821:Plants, Genetically Modified; D011740:Pyrimidine Dimers / Q000737:chemistry; D034622:RNA Interference; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; D002244:Carbon
keywords: UVB-tolerance; carbon-ion beam; rice mutant; root bending assay
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