title: Systemic biological analysis of the mutations in two distinct HIV-1mt genomes occurred during replication in macaque cells.
abstract: Fundamental property of viruses is to rapidly adapt themselves under changing conditions of virus replication. Using HIV-1 derivatives that poorly replicate in macaque cells as model viruses, we studied here mechanisms for promoting viral replication in non-natural host cells. We found that the HIV-1s could evolve to grow better in both macaque and human cells by the continuous culture in macaque lymphocyte cell lines. Notably, only several mutations at defined sites of the Pol-integrase and/or the Env-gp120 reproducibly appeared in repeated adaptation experiments and were sufficient to cause the phenotypic change. Meanwhile, no amino acid changes to enhance viral replication in macaque cells were found in interaction sites for the known anti-retroviral proteins. These findings disclose a hitherto unappreciated evolutionary pathway to augment HIV-1 replication in primate cells, where tuning of viral interactions with positive rather than negative factors for replication can play a dominant role.
authors: Department of Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
authors: Masako
authors: M
authors: Nomaguchi
authors: Naoya
authors: N
authors: Doi
authors: Sachi
authors: S
authors: Fujiwara
authors: Akatsuki
authors: A
authors: Saito
authors: Hirofumi
authors: H
authors: Akari
authors: Emi E
authors: EE
authors: Nakayama
authors: Tatsuo
authors: T
authors: Shioda
authors: Masaru
authors: M
authors: Yokoyama
authors: Hironori
authors: H
authors: Sato
authors: Akio
authors: A
authors: Adachi
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D002460:Cell Line; D004252:DNA Mutational Analysis; D016679:Genome, Viral; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008251:Macaca; D012692:Serial Passage; D014779:Virus Replication; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D015699:HIV Envelope Protein gp120; C514793:gp120 protein, Human immunodeficiency virus 1; D054302:pol Gene Products, Human Immunodeficiency Virus