title: Inoculum pretreatment affects bacterial survival, activity and catabolic gene expression during phytoremediation of diesel contaminated soil.
abstract: Plant-bacteria partnership is a promising approach for remediating soil contaminated with organic pollutants. The colonization and metabolic activity of an inoculated microorganism depend not only on environmental conditions but also on the physiological condition of the applied microorganisms. This study assessed the influence of different inoculum pretreatments on survival, gene abundance and catabolic gene expression of an applied strain (Pantoea sp. strain BTRH79) in the rhizosphere of ryegrass vegetated in diesel contaminated soil. Maximum bacterium survival, gene abundance and expression were observed in the soil inoculated with bacterial cells that had been pregrown on complex medium, and hydrocarbon degradation and genotoxicity reduction were also high in this soil. These findings propose that use of complex media for growing plant inocula may enhance bacterial survival and colonization and subsequently the efficiency of pollutant degradation.
authors: Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.
authors: Sumia
authors: S
authors: Khan
authors: Muhammad
authors: M
authors: Afzal
authors: Samina
authors: S
authors: Iqbal
authors: Muhammad Sajjad
authors: MS
authors: Mirza
authors: Qaiser M
authors: QM
authors: Khan
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015870:Gene Expression; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D008129:Lolium / Q000382:microbiology* / Q000502:physiology*; D008660:Metabolism / Q000235:genetics; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D006838:Hydrocarbons; D012987:Soil; D012989:Soil Pollutants