title: Expression of caveolin-1 in the early phase of beta-TCP implanted in dog mandible.
abstract: Caveolin is an essential and signature protein of caveolae. Caveolin-1 participates in signal transduction processes by acting as a scaffolding protein that concentrates, organizes and functional regulates signalling molecules within caveolar membranes. Beta-tricalcium phosphate (β-TCP) has been widely used for scaffold in tissue engineering due to its high biodegradability, osteoconductivity, easy manipulation, and lack of histotoxicity. To better understand the role of caveolin-1 in bone homeostasis and response to β-TCP scaffold, β-TCP was implanted into the dog mandible defects in beagle dogs, and gene expression profiles were examined focused on the molecular components involved in caveolin-1 regulation. Here we showed the quantitative imageology analysis characterized using in vivo micro-computed tomography (CT) images at 4 and 7 days after β-TCP implanted in dog mandibles. The bone reformation by using the β-TCP scaffolds began within 4 days of surgery, and was healing well at 7 days after surgery. Higher mRNA level of caveolin-1 was observed in β-TCP-implanted Beagle dog mandibles compared with controls at day 4 and day 7 post-surgery. The enhancement of caveolin-1 by β-TCP was further confirmed by immunohistochemistry and immunofluorescence analysis. We further revealed increased Smad7 and Phospho Stat3 expression in β-TCP-implanted specimens. Taken together, these results suggest that the enhancement of caveolin-1 play an important role in accelerating bone formation by β-TCP.
authors: Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.
authors: Cherng-Tzeh
authors: CT
authors: Chou
authors: Ujjal K
authors: UK
authors: Bhawal
authors: Nobuyuki
authors: N
authors: Watanabe
authors: Noboru
authors: N
authors: Kuboyama
authors: Wei-Jen
authors: WJ
authors: Chang
authors: Sheng-Yang
authors: SY
authors: Lee
authors: Yoshimitsu
authors: Y
authors: Abiko
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D018786:Bone Substitutes / Q000008:administration & dosage*; D002130:Calcium Phosphates / Q000008:administration & dosage*; D051242:Caveolin 1 / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D007150:Immunohistochemistry; D008334:Mandible / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000601:surgery*; D008422:Materials Testing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D051906:Smad7 Protein / Q000378:metabolism; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*; D059467:Transcriptome; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D051242:Caveolin 1; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D051906:Smad7 Protein; C485817:beta-tricalcium phosphate