title: Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus.
abstract: OBJECTIVE
  To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis.
  
  
  METHODS
  Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation study.
  
  
  RESULTS
  It was determined that PhoP and RovA recognized the promoter-proximal regions of psaEF and psaABC in order to repress and stimulate their transcription, respectively. The translation/transcription start sites, Shine-Dalgarno sequences (ribosomal binding site), core promoter -10 and -35 elements, PhoP and RovA sites and PhoP/RovA consensus-like sequences were identified to determine the structural organization of PhoP/RovA-dependent promoters of psaEF and psaABC.
  
  
  CONCLUSIONS
  RovA stimulated psaEF and psaABC, while PhoP repressed these two operons involving the direct association between RovA/PhoP and target promoter regions. The reciprocal regulation of psa genes by PhoP and RovA could contribute to the tightly controlled expression of the pH 6 antigen during infection.
authors: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors: Yiquan
authors: Y
authors: Zhang
authors: Li
authors: L
authors: Wang
authors: Nan
authors: N
authors: Fang
authors: Shi
authors: S
authors: Qu
authors: Yafang
authors: Y
authors: Tan
authors: Zhaobiao
authors: Z
authors: Guo
authors: Jingfu
authors: J
authors: Qiu
authors: Dongsheng
authors: D
authors: Zhou
authors: Ruifu
authors: R
authors: Yang
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis*; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D018983:DNA Footprinting; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015010:Yersinia pestis / Q000235:genetics*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C405626:RovA protein, Yersinia; D014157:Transcription Factors; C081814:pH 6 antigen, Yersinia; C061064:PhoP protein, Bacteria; D001616:beta-Galactosidase