title: [Study on effect of total coumarins from Urtica dentata on dextran sulfate sodium-induced colitis in mice].
abstract: OBJECTIVE
  To study the effect of total coumarins (TC) from Urtica dentata on dextran sulfate sodium (DSS)-induced colitis in mice.
  
  
  METHODS
  The colitis model was established by administering DSS. Having been treated with TC, their body weight was determined. Concentrations of IL-6, IL-10, TGF-beta1 and IFN-gamma were monitored by ELISA. Colon samples were collected for the histopathological examination. Western blot was used to detect TLR4 and NF-kappaB protein expression in colonic tissues.
  
  
  RESULTS
  TCs from U. dentata effectively controlled the body weight loss of mice with colitis, down-regulated the concentration of IL-6 and IFN-gamma and increased the suppressive cytokines IL-10 and TGF-beta1 in the serum. Additionally, TC alleviated the mucosal damage and decreased the expressions of TLR4 and NF-kappaB in colonic tissues.
  
  
  CONCLUSIONS
  TCs from U. dentata shows the anti-inflammatory effect on colitis in mice by reducing the expressions of TLR4 and NF-kappaB in colonic tissues and regulating pro-and anti-inflammatory cytokines.
authors: Department of Pharmacology, School of Pharmacy, Tongji Medical College, Wuhan 430030, China.
authors: Jing-Li
authors: JL
authors: Lu
authors: Wei-Jie
authors: WJ
authors: Li
authors: Wen-Rui
authors: WR
authors: Hou
authors: Yue
authors: Y
authors: Lan
authors: Hong
authors: H
authors: Zhou
authors: Li-Jiao
authors: LJ
authors: Yin
authors: Ying
authors: Y
authors: Zeng
authors: Ming
authors: M
authors: Xiang
mesh_terms: D000818:Animals; D003092:Colitis / Q000188:drug therapy* / Q000378:metabolism; D003374:Coumarins / Q000627:therapeutic use*; D016207:Cytokines / Q000097:blood; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors; D027003:Urticaceae / Q000737:chemistry*
chemical_list: D003374:Coumarins; D016207:Cytokines; D016328:NF-kappa B; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D016264:Dextran Sulfate