title: TBP dynamics during mouse oocyte meiotic maturation and early embryo development.
abstract: To maintain cell lineage, cells develop a mechanism which can transmit the gene activity information to the daughter cells. In mitosis, TBP (TATA-binding protein), a transcription factor which belongs to TFIID was associated with M phase chromosomes and was proved to be a bookmark for cellular memory. Although previous work showed that TBP was dispensable for mouse oocyte maturation and early embryo development, exogenous TBP protein was detected in the nuclear of oocytes and early embryos. It is still unknown whether exogenous TBP can associate with condensed chromosomes during meiosis and mouse early embryo development. In present study by the injection of GFP-tagged TBP mRNA we for the first time investigated TBP dynamics in mouse early embryos and confirmed its localization pattern in oocytes. The exogenous TBP enriched at germinal vesicle at GV stage but disappeared from the chromosomes after GVBD. Moreover, exogenous TBP was still dispersed from the chromosomes of somatic donor nuclear in oocytes by nuclear transfer (NT), further proving that oocyte has some mechanism to remove TBP. During mouse embryo development, the exogenous TBP was removed from the chromosomes of M phase zygotes, but was found to express weakly at the M phase of 2-cell. Moreover, in the blastocyst TBP was also detected at the M phase chromosomes. Overexpression of TBP caused the failure of oocyte maturation and embryo development. Our results supported the idea that TBP might be a marker for transmitting cellular memory to daughter cells.
authors: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: Shao-Chen
authors: SC
authors: Sun
authors: Xu-Guang
authors: XG
authors: Wang
authors: Xue-Shan
authors: XS
authors: Ma
authors: Xian-Ju
authors: XJ
authors: Huang
authors: Juan
authors: J
authors: Li
authors: Hong-Lin
authors: HL
authors: Liu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D047108:Embryonic Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D035181:TATA-Box Binding Protein; D049452:Green Fluorescent Proteins
references: Mol Biol Cell. 2002 Jan;13(1):276-84
references: 11809839
references: Nat Cell Biol. 2002 Jan;4(1):79-82
references: 11744923
references: Cell Cycle. 2011 Sep 15;10(18):3040-1
references: 21869611
references: Trends Biochem Sci. 2005 Nov;30(11):605-10
references: 16188444
references: Cell Cycle. 2011 Jun 1;10(11):1853-60
references: 21543895
references: Development. 1994 Aug;120(8):2347-57
references: 7925035
references: J Biol. 2009;8(11):97
references: 19951399
references: J Cell Biol. 2003 Jul 7;162(1):37-46
references: 12835313
references: Trends Biochem Sci. 1997 Jun;22(6):197-202
references: 9204705
references: Curr Biol. 2004 Apr 6;14(7):593-8
references: 15062100
references: Cell Cycle. 2005 Apr;4(4):513-5
references: 15753660
references: Nature. 1997 Aug 28;388(6645):895-9
references: 9278053
references: Genes Dev. 1996 Oct 1;10(19):2389-400
references: 8843192
references: Mol Cell. 2008 Oct 10;32(1):96-105
references: 18851836
references: Cell Cycle. 2011 Oct 15;10(20):3421-2
references: 22067653
references: Cell Cycle. 2011 Mar 15;10(6):871-2
references: 21368574
references: PLoS One. 2011 Apr 08;6(4):e18392
references: 21494665
references: Dev Biol. 2010 Mar 15;339(2):225-9
references: 19682982
references: Genes Dev. 2009 Sep 15;23(18):2210-23
references: 19759265
references: Nat Cell Biol. 2008 Nov;10(11):1318-23
references: 18931662
references: BMC Biol. 2009 Aug 03;7:45
references: 19650908
references: Reproduction. 2007 Jul;134(1):51-62
references: 17641088
references: Genes Dev. 2007 Sep 1;21(17):2113-7
references: 17785521