title: Development of a simple method for detection of the HLA-A*31:01 allele.
abstract: It is known that rare but severe cutaneous adverse drug reactions (cADRs), such as Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis (TEN) and drug-induced hypersensitivity syndrome (DIHS), are induced by carbamazepine (CBZ). Recent studies have shown an association between HLA-A*31:01 and CBZ-induced severe cADRs in Japanese and Caucasian populations. In this study, we developed a simple method to detect the HLA-A*31:01 allele by nested allele-specific primer-polymerase chain reaction combined with restriction fragment length polymorphism analysis. Accuracy of the developed method was evaluated by direct sequencing analysis of PCR products amplified from DNA samples with known HLA-A genotypes and by consigning diagnosis of DNA samples with unknown HLA-A genotypes to a company providing clinical laboratory testing. The method developed in this study is simple, rapid, and of low cost compared to outsourcing tests and may be useful for in-house testing of the HLA-A*31:01 allele.
authors: Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Chiba University.
authors: Kazuki
authors: K
authors: Uchiyama
authors: Fumika
authors: F
authors: Kubota
authors: Noritaka
authors: N
authors: Ariyoshi
authors: Jun
authors: J
authors: Matsumoto
authors: Itsuko
authors: I
authors: Ishii
authors: Mitsukazu
authors: M
authors: Kitada
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D002220:Carbamazepine / Q000009:adverse effects; D060005:Genotyping Techniques / Q000379:methods*; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013262:Stevens-Johnson Syndrome / Q000276:immunology / Q000517:prevention & control
chemical_list: D015234:HLA-A Antigens; C069527:HLA-A31 antigen; D002220:Carbamazepine