title: [Changes of cytokines and protein expression of macrophages stimulated with exosome from macrophages by Mycobacterium  avium  infection].
abstract: OBJECTIVE
  To explore the mechanism underlying the molecular immune response of macrophages stimulated with exosome [(+)exosome] from macrophages after Mycobacterium avium (M. avium) infection and analyze the differential protein component of the exosome.
  
  
  METHODS
  The culture supernatants of M.avium-infected macrophages and uninfected ones were collected and exosome was harvested from the supernatants by frozen ultra centrifugation. The concentrations of IFN-γ, TNF-α in supernatants were detected by enzyme-linked immunosorbent assay (ELISA), and CD80, CD86 expressions on macrophages were analyzed by flow cytometry after macrophages were stimulated with exosome. Meanwhile, 2-DE MALDI TOF/TOF MS was used to identify differentially expressed proteins of exosome between M. avium infected group and uninfected group.
  
  
  RESULTS
  IFN-γ, TNF-α concentrations were increased in the supernatant after stimulated with (+)exosome and CD80, CD86 were raised on macrophage surface by stimulation with (+)exosome. With 2-DE MALDI TOF/TOF MS analysis, we obtained 18 differentially expressed proteins and 12 proteins were identified successfully.
  
  
  CONCLUSIONS
  (+)exosome induces TNF-α, IFN-γ secretion from macrophages and result in the promotion of inflammatory response. In addition, (+)exosome enhances CD80 and CD86 protein expressions. The function of the differentially expressed proteins we identified is closely related to cytoskeleton, protein synthesis and processing, inflammatory response.
authors: Central South University, Changsha, China. wangjianjun0520@163.com
authors: Jianjun
authors: J
authors: Wang
authors: Cai
authors: C
authors: Chen
authors: Pingfang
authors: P
authors: Xie
authors: Yi
authors: Y
authors: Pan
authors: Yunhong
authors: Y
authors: Tan
authors: Lijun
authors: L
authors: Tang
mesh_terms: D016207:Cytokines / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D055354:Exosomes / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D015270:Mycobacterium avium-intracellulare Infection / Q000276:immunology*; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D016207:Cytokines; D011506:Proteins