title: Production of sophorolipids with eicosapentaenoic acid and docosahexaenoic acid from Wickerhamiella domercqiae var. sophorolipid using fish oil as a hydrophobic carbon source.
abstract: Sophorolipids (SLs) were synthesized by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 grown on fish oil and glucose. They were purified using preparative HPLC and their structures were identified by MS/MS. The yields of total and lactonic SLs were 47 and 19 g l(-1) in shake-flasks when fish oil 4 % (v/v) was used with glucose in the medium. The composition of SL mixture contained more than 20 SL molecules. Several unconventional SL molecules with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) including zero-, mono- and di-acetylated acidic SLs with EPA and a di-acetylated acidic SL with DHA were obtained. Two unconventional lactonic SL molecules, non-acetylated lactonic SL with 22:3 and non-acetylated lactonic SL with 20:0, were also obtained.
authors: State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road 27, Jinan, 250100, Shandong, China. sdulihui@163.com
authors: Hui
authors: H
authors: Li
authors: Xiao-Jing
authors: XJ
authors: Ma
authors: Shuang
authors: S
authors: Wang
authors: Xin
authors: X
authors: Song
mesh_terms: D002244:Carbon / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005395:Fish Oils / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006017:Glycolipids / Q000302:isolation & purification / Q000378:metabolism*; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005395:Fish Oils; D006017:Glycolipids; D004281:Docosahexaenoic Acids; D002244:Carbon; D015118:Eicosapentaenoic Acid; D005947:Glucose