title: Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.
abstract: Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the mechanisms involved in resistance to pathogens. We report here the detection of QTL for resistance to Viral Haemorrhagic Septicaemia Virus (VHSV), a major threat for European aquaculture industry. Two induced mitogynogenetic doubled haploid F2 rainbow trout (Oncorhynchus mykiss) families were used. These families combined the genome of susceptible and resistant F0 breeders and contained only fully homozygous individuals. For phenotyping, fish survival after an immersion challenge with the virus was recorded, as well as in vitro virus replication on fin explants. A bidirectional selective genotyping strategy identified seven QTL associated to survival. One of those QTL was significant at the genome-wide level and largely explained both survival and viral replication in fin explants in the different families of the design (up to 65% and 49% of phenotypic variance explained respectively). These results evidence the key role of innate defence in resistance to the virus and pave the way for the identification of the gene(s) responsible for resistance. The identification of a major QTL also opens appealing perspectives for selective breeding of fish with improved resistance.
authors: INRA, UMR 1313 Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
authors: Eloi R
authors: ER
authors: Verrier
authors: Michel
authors: M
authors: Dorson
authors: Stéphane
authors: S
authors: Mauger
authors: Corinne
authors: C
authors: Torhy
authors: Céline
authors: C
authors: Ciobotaru
authors: Caroline
authors: C
authors: Hervet
authors: Nicolas
authors: N
authors: Dechamp
authors: Carine
authors: C
authors: Genet
authors: Pierre
authors: P
authors: Boudinot
authors: Edwige
authors: E
authors: Quillet
mesh_terms: D000818:Animals; D017756:Aquaculture; D002874:Chromosome Mapping; D005260:Female; D016678:Genome; D005838:Genotype; D031941:Hemorrhagic Septicemia, Viral / Q000235:genetics* / Q000276:immunology / Q000401:mortality / Q000821:virology; D006720:Homozygote; D007113:Immunity, Innate / Q000235:genetics*; D008297:Male; D029141:Novirhabdovirus / Q000502:physiology*; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology / Q000821:virology; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000276:immunology*; D015996:Survival Rate; D046509:Tissue Culture Techniques; D014779:Virus Replication
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