title: Down-regulation of miR-106b suppresses the growth of human glioma cells.
abstract: Recently, many studies have found that the miR-106b ~25 cluster plays an oncogenic role in tumor progression. However, the precise role of each microRNAs (miRNAs) in the cluster is not yet clear. In the present study, we examined the expression of miR-106b in glioma samples and a tissue microarray by real-time PCR and in situ hybridization (ISH), respectively, finding that miR-106b is overexpressed in the majority of gliomas. Meanwhile, the expression of miR-106b was positively correlated with tumor grade (p < 0.05). The transfection of a miR-106b anti-sense oligonucleotide (ASON) into three human glioma cell lines (U251, LN229 and TJ905) suppressed the proliferation of these cells. Moreover, the growth of xenograft tumors in nude mice treated with miR-106b ASON was significantly impaired. A bioinformatics analysis predicted that RBL2 may be the target of miR-106b, and dual-luciferase reporter assays identified RBL2, but not RB1 or RBL1, as a target of miR-106b. These results suggest that miR-106b facilitates glioma cell growth by promoting cell cycle progression through the negative regulation of RBL2.
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, People's Republic of China.
authors: Anling
authors: A
authors: Zhang
authors: Jianwei
authors: J
authors: Hao
authors: Kun
authors: K
authors: Wang
authors: Qiang
authors: Q
authors: Huang
authors: Kai
authors: K
authors: Yu
authors: Chunsheng
authors: C
authors: Kang
authors: Guangxiu
authors: G
authors: Wang
authors: Zhifan
authors: Z
authors: Jia
authors: Lei
authors: L
authors: Han
authors: Peiyu
authors: P
authors: Pu
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D060787:Neoplasm Grading; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism; D050720:Retinoblastoma-Like Protein p107 / Q000235:genetics / Q000378:metabolism; D050717:Retinoblastoma-Like Protein p130 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D023041:Xenograft Model Antitumor Assays
chemical_list: C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; C493844:RBL1 protein, human; C493837:RBL2 protein, human; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D050720:Retinoblastoma-Like Protein p107; D050717:Retinoblastoma-Like Protein p130
references: J Mol Med (Berl). 2009 Jan;87(1):43-51
references: 18810376
references: Cancer Cell. 2008 Mar;13(3):272-86
references: 18328430
references: Cell. 2008 Mar 7;132(5):875-86
references: 18329372
references: Nucleic Acids Res. 2008 Jan;36(Database issue):D154-8
references: 17991681
references: Oncogene. 2005 Apr 18;24(17):2796-809
references: 15838516
references: J Cell Biol. 2011 Aug 8;194(3):407-23
references: 21825075
references: Oncogene. 2003 Oct 23;22(48):7632-41
references: 14576826
references: Int J Oncol. 2009 Jun;34(6):1653-60
references: 19424584
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: J Neurooncol. 2009 May;92(3):297-306
references: 19357957
references: Med Oncol. 2011 Mar;28(1):7-14
references: 20077038
references: Cancer Lett. 2008 Dec 18;272(2):197-205
references: 19013014
references: Cell. 2008 Mar 7;132(5):860-74
references: 18329371
references: J Pathol. 2008 Jul;215(3):253-62
references: 18438954
references: Lab Invest. 2010 Feb;90(2):144-55
references: 20048743
references: EMBO J. 2011 Aug 19;30(20):4309-22
references: 21857646
references: Brain Res. 2010 Nov 4;1359:14-21
references: 20816946
references: Biochem Biophys Res Commun. 2005 Sep 9;334(4):1351-8
references: 16039986
references: Chin Med J (Engl). 2009 Oct 20;122(20):2405-11
references: 20079150
references: Mol Cell Biol. 2008 Apr;28(7):2167-74
references: 18212054
references: Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2999-3004
references: 14973191
references: Curr Opin Pharmacol. 2009 Dec;9(6):727-32
references: 19773200
references: Mod Pathol. 2010 Oct;23(10):1404-17
references: 20711171
references: J Dig Dis. 2010 Feb;11(1):50-4
references: 20132431
references: PLoS One. 2011 Jan 20;6(1):e16138
references: 21283765
references: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references: 15761078
references: Mol Cells. 2006 Jun 30;21(3):356-9
references: 16819297
references: Clin Cancer Res. 2010 Feb 15;16(4):1129-39
references: 20145181
references: J Clin Neurosci. 2010 Apr;17(4):417-21
references: 20167494