title: Glycoprotein 130 promotes human blastocyst development in vitro.
abstract: OBJECTIVE
  To investigate the efficacy of leukemia inhibitory factor (LIF) and/or glycoprotein 130 (gp130) on in vitro growth of human embryos.
  
  
  METHODS
  Laboratory study.
  
  
  METHODS
  University hospital-based IVF clinic.
  
  
  METHODS
  A total of 164 frozen embryos that survived thawing were cultured in media supplemented with LIF and/or gp130 or control media.
  
  
  METHODS
  Morphological development was evaluated by light microscopy. Protein expression profiles of single blastocysts were evaluated using matrix-assisted laser desorption/ionization time of flight-based intact cell mass spectrometry.
  
  
  METHODS
  Embryo development and protein content.
  
  
  RESULTS
  Addition of gp130 to culture media improved blastocyst formation (73% vs. 43%). Addition of LIF to the culture media did not improve embryo development. Protein fingerprint spectra were obtained that revealed significant intensity changes for multiple molecular species including thymosin beta-10, thymosin beta-4, histone H2A, histone H2B, histone H4, ubiquitin, ubiquitin-T, and acyl-CoA binding protein.
  
  
  CONCLUSIONS
  Glycoprotein 130, but not LIF, seems to be beneficial for preimplantation embryo development, implicating a physiological role in regulating preimplantation development in humans and thus ought to be included in culture media designed for embryo culture to the blastocyst stage. Furthermore, these findings highlight the great potential of matrix-assisted laser desorption/ionization time of flight mass spectrometry and intact cell mass spectrometry as a versatile tool in reproductive medicine research.
authors: Department of Clinical Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.
authors: Fredwell
authors: F
authors: Hambiliki
authors: Jörg
authors: J
authors: Hanrieder
authors: Jonas
authors: J
authors: Bergquist
authors: Julius
authors: J
authors: Hreinsson
authors: Anneli
authors: A
authors: Stavreus-Evers
authors: Kjell
authors: K
authors: Wånggren
mesh_terms: D001755:Blastocyst / Q000502:physiology*; D046149:Embryo Culture Techniques / Q000379:methods; D004624:Embryo Transfer / Q000379:methods; D047108:Embryonic Development / Q000502:physiology*; D005307:Fertilization in Vitro / Q000379:methods; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D008297:Male
chemical_list: D006023:Glycoproteins; C480482:glycoprotein 130, human