title: GLUCAN SYNTHASE-LIKE8 and STEROL METHYLTRANSFERASE2 are required for ploidy consistency of the sexual reproduction system in Arabidopsis.
abstract: In sexually reproducing plants, the meiocyte-producing archesporal cell lineage is maintained at the diploid state to consolidate the formation of haploid gametes. In search of molecular factors that regulate this ploidy consistency, we isolated an Arabidopsis thaliana mutant, called enlarged tetrad2 (et2), which produces tetraploid meiocytes through the stochastic occurrence of premeiotic endomitosis. Endomitotic polyploidization events were induced by alterations in cell wall formation, and similar cytokinetic defects were sporadically observed in other tissues, including cotyledons and leaves. ET2 encodes GLUCAN SYNTHASE-LIKE8 (GSL8), a callose synthase that mediates the deposition of callose at developing cell plates, root hairs, and plasmodesmata. Unlike other gsl8 mutants, in which defects in cell plate formation are seedling lethal, cytokinetic defects in et2 predominantly occur in flowers and have little effect on vegetative growth and development. Similarly, mutations in STEROL METHYLTRANSFERASE2 (SMT2), a major sterol biosynthesis enzyme, also lead to weak cytokinetic defects, primarily in the flowers. In addition, SMT2 allelic mutants also generate tetraploid meiocytes through the ectopic induction of premeiotic endomitosis. These observations demonstrate that appropriate callose and sterol biosynthesis are required for maintaining the ploidy level of the premeiotic germ lineage and that subtle defects in cytokinesis may lead to diploid gametes and polyploid offspring.
authors: In Vitro Biology and Horticulture, Department of Plant Production, University of Ghent, 9000 Ghent, Belgium.
authors: Nico
authors: N
authors: De Storme
authors: Joachim
authors: J
authors: De Schrijver
authors: Wim
authors: W
authors: Van Criekinge
authors: Vera
authors: V
authors: Wewer
authors: Peter
authors: P
authors: Dörmann
authors: Danny
authors: D
authors: Geelen
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000166:cytology / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis / Q000235:genetics; D009154:Mutation; D054046:Plant Stomata / Q000235:genetics; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism; D057891:Tetraploidy
chemical_list: D029681:Arabidopsis Proteins; D013261:Sterols; D008780:Methyltransferases; C585557:SMT3 protein, Arabidopsis; C481389:sterol methyltransferase 2, Arabidopsis; D005964:Glucosyltransferases; C549616:chorus protein, Arabidopsis
references: Development. 2010 May;137(10):1731-41
references: 20430748
references: Cell. 1975 Jun;5(2):123-9
references: 1170022
references: Plant Physiol. 2006 Nov;142(3):1004-13
references: 16980565
references: Plant Physiol. 2001 Jun;126(2):509-16
references: 11402182
references: Development. 1997 Feb;124(3):665-72
references: 9043081
references: Cell. 1994 May 6;77(3):371-9
references: 8181057
references: Science. 1994 Jun 3;264(5164):1458-60
references: 8197459
references: Curr Opin Plant Biol. 2003 Dec;6(6):544-53
references: 14611952
references: Cell. 1991 Jun 28;65(7):1177-89
references: 1905980
references: Plant Physiol. 1991 Jul;96(3):985-9
references: 16668285
references: J Cell Biol. 1997 Dec 15;139(6):1485-93
references: 9396754
references: Plant Physiol. 2008 Aug;147(4):1544-52
references: 18678745
references: Curr Biol. 2002 Jan 22;12(2):153-8
references: 11818068
references: Science. 1983 Jun 3;220(4601):1049-51
references: 17754551
references: Eur J Cell Biol. 1999 Feb;78(2):100-8
references: 10099932
references: Blood. 2008 Oct 15;112(8):3164-74
references: 18684864
references: Plant Cell. 2003 Nov;15(11):2503-13
references: 14555698
references: Nat Genet. 2005 May;37(5):501-6
references: 15806101
references: Plant J. 1998 Aug;15(3):321-32
references: 9750345
references: J Cell Biol. 2001 Feb 5;152(3):531-43
references: 11157980
references: Curr Biol. 2009 Mar 24;19(6):518-23
references: 19268593
references: Genes Dev. 2009 Nov 1;23(21):2461-77
references: 19884253
references: BMC Plant Biol. 2011 Jun 12;11:103
references: 21663695
references: Plant Cell. 2011 Dec;23(12):4382-93
references: 22167058
references: Development. 1994 Dec;120(12):3367-77
references: 7821209
references: J Cell Biol. 1995 Sep;130(6):1345-57
references: 7559757
references: J Lipid Res. 2011 May;52(5):1039-54
references: 21382968
references: Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:751-784
references: 11337415
references: Plant J. 2008 Jun;54(5):911-23
references: 18315544
references: Development. 2003 Sep;130(17):4011-24
references: 12874123
references: Development. 1997 Jul;124(13):2645-57
references: 9217006
references: Plant Cell. 2004 Mar;16(3):643-57
references: 15004270
references: Plant Physiol. 2002 Sep;130(1):312-24
references: 12226511
references: Plant Cell Rep. 1994 Aug;13(11):652-6
references: 24196247
references: Plant Signal Behav. 2009 Jun;4(6):489-92
references: 19816126
references: Nat Methods. 2009 Aug;6(8):550-1
references: 19644454
references: J Exp Bot. 2011 Mar;62(5):1659-68
references: 21109579
references: BMC Plant Biol. 2005 Oct 07;5:22
references: 16212660
references: PLoS Genet. 2009 Mar;5(3):e1000430
references: 19300502
references: Mol Gen Genet. 1996 Dec 13;253(3):267-77
references: 9003313
references: Plant Physiol. 2009 May;150(1):105-13
references: 19286936
references: Plant Cell. 2002 Sep;14(9):2045-58
references: 12215504
references: Plant Cell. 2001 Apr;13(4):755-68
references: 11283334
references: Plant Physiol. 2010 Jun;153(2):741-56
references: 20421456
references: Plant Physiol. 2011 Mar;155(3):1403-15
references: 21257792
references: J Exp Bot. 2007;58(13):3657-70
references: 18057042
references: J Exp Zool A Comp Exp Biol. 2006 Jun 1;305(6):513-23
references: 16526047
references: J Exp Bot. 2005 Apr;56(414):1263-8
references: 15737981
references: Plant Mol Biol. 2005 Jun;58(3):333-49
references: 16021399
references: Cell. 1996 Jan 12;84(1):61-71
references: 8548827
references: Somatic Cell Genet. 1976 Sep;2(5):387-400
references: 1027149
references: Plant Mol Biol. 2001 Dec;47(6):693-701
references: 11785931
references: Curr Opin Plant Biol. 2003 Feb;6(1):57-62
references: 12495752
references: Plant J. 2009 Apr;58(1):13-26
references: 19067977
references: Plant Cell. 2006 Oct;18(10):2443-51
references: 17028205
references: J Exp Bot. 1999 Sep;50(338):1437-46
references: 11543207
references: Plant J. 2004 Apr;38(2):227-43
references: 15078327
references: Plant J. 2005 May;42(3):315-28
references: 15842618
references: J Clin Invest. 2012 Mar;122(3):844-58
references: 22293177
references: Plant Mol Biol. 2000 Aug;43(5-6):735-45
references: 11089873
references: PLoS Genet. 2012;8(4):e1002679
references: 22570622
references: Plant Cell. 2001 Apr;13(4):769-79
references: 11283335
references: Cell. 2001 May 4;105(3):297-306
references: 11348589
references: Plant Physiol. 2012 Dec;160(4):1808-26
references: 23096158