title: Cyclic sample pooling using two-dimensional liquid chromatography system enhances coverage in shotgun proteomics.
abstract: We report a cyclic sample pooling technique devised in two-dimensional liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) shotgun proteomics that renders deeper proteome coverage; we combined low pH reversed-phase (RP) LC in trifluoroacetic acid in the first dimension, followed by cyclic sample pooling of the eluate and low-pH RP-LC in formic acid in the second dimension. The new protocol has a significantly higher resolving power suitable for LC-ESI-MS/MS shotgun proteomics.
authors: Center for Disease Proteomics, School of Science, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara-shi, Kanagawa, 252-0373, Japan.
authors: Yusuke
authors: Y
authors: Kawashima
authors: Mamoru
authors: M
authors: Satoh
authors: Tatsuya
authors: T
authors: Saito
authors: Takashi
authors: T
authors: Matsui
authors: Fumio
authors: F
authors: Nomura
authors: Hiroyuki
authors: H
authors: Matsumoto
authors: Yoshio
authors: Y
authors: Kodera
mesh_terms: D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D005561:Formates / Q000737:chemistry; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D005561:Formates; D010446:Peptide Fragments; C030544:formic acid; D014269:Trifluoroacetic Acid; D014357:Trypsin