title: Complex evolutionary patterns of two rare human G3P[9] rotavirus strains possessing a feline/canine-like H6 genotype on an AU-1-like genotype constellation.
abstract: The group A rotavirus (RVA) G3P[9] is a rare VP7-VP4 genotype combination, detected occasionally in humans and cats. Other than the prototype G3P[9] strain, RVA/Human- tc/JPN/AU-l/1982/G3P3[9], the whole genomes of only two human G3P[9] RVA strains and two feline G3P[9] RVA strains have been analyzed so far, revealing complex evolutionary patterns, distinct from that of AU-1. We report here the whole genomic analyses of two human G3P[9] RVA strains, RVA/Human-tc/CHN/L621/2006/G3P[9] and RVA/Human-wt/CHN/E2451/2011/G3P[9], detected in patients with diarrhea in China. Strains L621 and E2451 possessed a H6 NSP5 genotype on an AU-1-like genotype constellation, not reported previously. However, not all the genes of L621 and E2451 were closely related to those of AU-1, or to each other, revealing different evolutionary patterns among the AU-1-like RVAs. The VP7, VP4, VP6 and NSP4 genes of E2451 and L621 were found to cluster together with human G3P[9] RVA strains believed to be of possible feline/canine origin, and feline or raccoon dog RVA strains. The VP1, VP3, NSP2 and NSP5 genes of E2451 and L621 formed distinct clusters in genotypes typically found in feline/canine RVA strains or RVA strains from other host species which are believed to be of feline/canine RVA origin. The VP2 genes of E2451 and L621, and NSP3 gene of L621 clustered among RVA strains from different host species which are believed to have a complete or partial feline/canine RVA origin. The NSP1 genes of E2451 and L621, and NSP3 gene of E2451 clustered with AU-1 and several other strains possessing a complete or partial feline RVA strain BA222-05-like genotype constellation. Taken together, these observations suggest that nearly all the eleven gene segments of G3P[9] RVA strains L621 and E2451 might have originated from feline/canine RVAs, and that reassortments may have occurred among these feline/canine RVA strains, before being transmitted to humans.
authors: Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, China. wyhhyc@yahoo.com.cn
authors: Yuan-Hong
authors: YH
authors: Wang
authors: Bei-Bei
authors: BB
authors: Pang
authors: Xuan
authors: X
authors: Zhou
authors: Souvik
authors: S
authors: Ghosh
authors: Wei-Feng
authors: WF
authors: Tang
authors: Jin-Song
authors: JS
authors: Peng
authors: Quan
authors: Q
authors: Hu
authors: Dun-Jin
authors: DJ
authors: Zhou
authors: Nobumichi
authors: N
authors: Kobayashi
mesh_terms: D000818:Animals; D002415:Cats; D002675:Child, Preschool; D004285:Dogs; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000235:genetics / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins