title: Implantable patch sensor for L-glutamate in hippocampal slices.
abstract: We describe an implantable patch sensor for monitoring L-glutamate in hippocampal slices in submerged and interface preparations. This patch sensor is prepared by excising the cell membrane in the CA3 region of a hippocampal slice in a submerged preparation, and then implanted in the target neuronal region (CA1) of mouse hippocampal slices. The lifetime of the sensor was 3-8 min for the interface slice and 40-50 min for the submerged slice. The calibration of an implanted sensor can be achieved by adding an L-glutamate solution to a bath (ACSF) solution. The monitoring of L-glutamate release in the CA1 region of mouse hippocampal slices under chemical stimulation with γ-aminobutyric acid (GABA) and potassium chloride (KCl) was demonstrated.
authors: Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan.
authors: Natsumi
authors: N
authors: Soma
authors: Narumi
authors: N
authors: Watanabe
authors: Atsushi
authors: A
authors: Shoji
authors: Masao
authors: M
authors: Sugawara
mesh_terms: D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D018408:Patch-Clamp Techniques / Q000295:instrumentation*; D019736:Prostheses and Implants
chemical_list: D018698:Glutamic Acid