title: A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle.
abstract: In budding yeast, association of the Est1 regulatory protein with telomerase is thought to be limited to the late S phase, when telomere elongation occurs. By monitoring the stoichiometry of telomerase subunits, we show instead that a telomerase complex containing Est1 is assembled much earlier in the cell cycle. We also report a biochemical interaction between Est1 and the telomere binding protein Cdc13 that recapitulates the previously observed genetic relationship between EST1 and CDC13. This supports a model in which regulated binding of Cdc13 to chromosome termini dictates subsequent interaction of a recruitment-competent telomerase complex with telomeres.
authors: Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
authors: Timothy M
authors: TM
authors: Tucey
authors: Victoria
authors: V
authors: Lundblad
mesh_terms: D015825:Chromosomes, Fungal; D000939:Epitopes / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016615:Telomere / Q000235:genetics / Q000378:metabolism; D034501:Telomere-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C452219:Cdc13 protein, S cerevisiae; D000939:Epitopes; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins; C099024:EST1 protein, S cerevisiae; C509185:EST2 protein, S cerevisiae; D019098:Telomerase
references: Cell. 2004 Apr 30;117(3):323-35
references: 15109493
references: RNA. 2012 Sep;18(9):1597-604
references: 22847816
references: PLoS Genet. 2008 Oct;4(10):e1000236
references: 18949040
references: Cell. 1989 May 19;57(4):633-43
references: 2655926
references: Science. 1995 Jul 21;269(5222):396-400
references: 7618104
references: Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20362-9
references: 21969561
references: Mol Cell. 2004 Oct 8;16(1):139-46
references: 15469829
references: Yeast. 2007 Jan;24(1):39-45
references: 17192851
references: RNA. 2006 Sep;12(9):1721-37
references: 16894218
references: Science. 1996 Oct 11;274(5285):249-52
references: 8824190
references: Science. 2002 Aug 9;297(5583):1023-6
references: 12169735
references: Cell. 2001 Feb 9;104(3):387-96
references: 11239396
references: Cell. 2009 Jan 9;136(1):50-61
references: 19135888
references: Curr Biol. 2000 Apr 20;10(8):487-90
references: 10801419
references: PLoS Genet. 2011 May;7(5):e1002060
references: 21573139
references: Nucleic Acids Res. 2002 Oct 1;30(19):4305-13
references: 12364610
references: Science. 1999 Oct 1;286(5437):117-20
references: 10506558
references: Mol Cell. 2011 Dec 9;44(5):819-27
references: 22152484
references: Mol Cell. 2004 May 21;14(4):515-22
references: 15149600
references: Nat Struct Mol Biol. 2006 Aug;13(8):720-8
references: 16862158