title: Transformation of Candida guilliermondii wild-type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin-resistant marker.
abstract: We designed an efficient transformation system for Candida guilliermondii wild-type strains. We demonstrated that the Staphylococcus aureus MRSA 252 ble coding sequence placed under the control of the yeast phosphoglycerate kinase gene transcription-regulating regions confers phleomycin resistance to transformed C. guilliermondii cells. To illustrate the potential of this drug-resistant cassette, we carried out the disruption of the C. guilliermondii ADE2 gene. This new dominant selectable marker represents a powerful tool to study the function of various genes in this yeast of clinical and biotechnological interest.
authors: EA2106, Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François-Rabelais de Tours, Tours, France.
authors: Emilien
authors: E
authors: Foureau
authors: Vincent
authors: V
authors: Courdavault
authors: Andrew J
authors: AJ
authors: Simkin
authors: Andriy A
authors: AA
authors: Sibirny
authors: Joël
authors: J
authors: Crèche
authors: Nathalie
authors: N
authors: Giglioli-Guivarc'h
authors: Marc
authors: M
authors: Clastre
authors: Nicolas
authors: N
authors: Papon
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002175:Candida / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004352:Drug Resistance, Microbial; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics; D008969:Molecular Sequence Data; D010692:Phleomycins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004271:DNA, Fungal; D010692:Phleomycins; D011994:Recombinant Proteins