title: Bacillus thermocopriae sp. nov., isolated from a compost.
abstract: A Gram-reaction-positive, facultatively anaerobic, motile, endospore-forming, rod-shaped strain, designated SgZ-7(T), was isolated from a windrow compost pile and was characterized by means of a polyphasic approach. Growth occurred with 0-3 % (w/v) NaCl (optimum 1 %), at pH 6.0-10.0 (optimum pH 7.2) and at 40-60 °C (optimum 50 °C). The main respiratory quinone was MK-7. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The DNA G+C content was 46.6 mol%. The phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain SgZ-7(T) should be assigned to the genus Bacillus and was related most closely to Bacillus drentensis LMG 21831(T) (sequence similarity 97.2 %). The result of the DNA-DNA hybridization experiment revealed a low relatedness (27.2 %) between the isolate and B. drentensis LMG 21831(T). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain SgZ-7(T) represents a novel species, for which the name Bacillus thermocopriae sp. nov. is proposed. The type strain is SgZ-7(T) (= CCTCC AB 2012030(T) = KACC 16700(T)).
authors: Chemistry and Materials Institute, Sichuan Normal University, Chengdu 610068, PR China.|Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
authors: Luchao
authors: L
authors: Han
authors: Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
authors: Guiqin
authors: G
authors: Yang
authors: 101 Research Institute of the Ministry of Civil Affairs, Beijing 100070, PR China.
authors: Xuemei
authors: X
authors: Zhou
authors: 101 Research Institute of the Ministry of Civil Affairs, Beijing 100070, PR China.
authors: Dehui
authors: D
authors: Yang
authors: Chemistry and Materials Institute, Sichuan Normal University, Chengdu 610068, PR China.
authors: Pei
authors: P
authors: Hu
authors: Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
authors: Qin
authors: Q
authors: Lu
authors: Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
authors: Shungui
authors: S
authors: Zhou
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7