title: An automated system for high-throughput single cell-based breeding.
abstract: When establishing the most appropriate cells from the huge numbers of a cell library for practical use of cells in regenerative medicine and production of various biopharmaceuticals, cell heterogeneity often found in an isogenic cell population limits the refinement of clonal cell culture. Here, we demonstrated high-throughput screening of the most suitable cells in a cell library by an automated undisruptive single-cell analysis and isolation system, followed by expansion of isolated single cells. This system enabled establishment of the most suitable cells, such as embryonic stem cells with the highest expression of the pluripotency marker Rex1 and hybridomas with the highest antibody secretion, which could not be achieved by conventional high-throughput cell screening systems (e.g., a fluorescence-activated cell sorter). This single cell-based breeding system may be a powerful tool to analyze stochastic fluctuations and delineate their molecular mechanisms.
authors: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan. n-yosi44@agr.nagoya-u.ac.jp
authors: Nobuo
authors: N
authors: Yoshimoto
authors: Akiko
authors: A
authors: Kida
authors: Xu
authors: X
authors: Jie
authors: Masaya
authors: M
authors: Kurokawa
authors: Masumi
authors: M
authors: Iijima
authors: Tomoaki
authors: T
authors: Niimi
authors: Andrés D
authors: AD
authors: Maturana
authors: Itoshi
authors: I
authors: Nikaido
authors: Hiroki R
authors: HR
authors: Ueda
authors: Kenji
authors: K
authors: Tatematsu
authors: Katsuyuki
authors: K
authors: Tanizawa
authors: Akihiko
authors: A
authors: Kondo
authors: Ikuo
authors: I
authors: Fujii
authors: Shun'ichi
authors: S
authors: Kuroda
mesh_terms: D000818:Animals; D001331:Automation; D016466:CHO Cells; D045744:Cell Line, Tumor; D002469:Cell Separation; D006224:Cricetinae; D003412:Cricetulus; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D057809:HEK293 Cells; D057166:High-Throughput Screening Assays; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000276:immunology; D051379:Mice; D011817:Rabbits; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; C013830:baysilon; D007770:L-Lactate Dehydrogenase
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