title: Novel insights regarding the sigmoidal pattern of resistance to neomycin conferred by the aphII gene, in Streptomyces lividans.
abstract: A library of synthetic promoters of various strengths, specifically constructed for Streptomyces species, was cloned in the promoter-probe plasmid pIJ487, upstream of the promoter-less aphII gene that confers resistance to neomycin. The survival rates conferred by promoters were assessed in the presence of 100 μg.ml-1 neomycin. The correlation between the transcriptional activity of the aphII gene (estimated by RT-PCR) and the resistance to neomycin (expressed as survival rate) indicated a sigmoid rather than a linear correlation. In this issue, we propose a tentative explanation for this sigmoidal pattern of resistance in relation with the level of aphII gene expression. Beyond this specific example, our model might constitute a sound explanation for the generally observed but never explained sigmoidal shape of classical inhibition curves obtained in the presence of linearly increasing antibiotic concentrations.
authors: Institut de Génétique et Microbiologie, UMR8621 CNRS Université Paris-Sud, Orsay, 91405, France. marie-joelle.virolle@igmors.u-psud.fr.
authors: Nicolas
authors: N
authors: Seghezzi
authors: Marie-Joelle
authors: MJ
authors: Virolle
authors: Patrick
authors: P
authors: Amar
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