title: In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy.
abstract: The authors demonstrate in vivo dynamic process imaging using a label-free real-time optical-resolution photoacoustic microscope (OR-PAM). This reflection-mode system takes advantage of a 532-nm fiber laser source with a high pulse repetition rate of up to 600 kHz combined with a fast-scanning mirror system. Microvasculature in SCID mouse ears is imaged at near real-time (0.5 fps) for a 1×1 mm2 field of view (FOV) with micron-scale lateral resolution. We also demonstrate imaging of cardiac-induced microhemodynamics in murine microvasculature at real-time frame-rates (30 fps) over a 250×250 μm2 FOV using real-time C-scan OR-PAM with ability to provide sustained imaging with near real-time feedback for focusing and positioning.
authors: University of Alberta, Department of Electrical and Computer Engineering, Edmonton, Alberta, Canada, T6G 2V4.
authors: Wei
authors: W
authors: Shi
authors: Peng
authors: P
authors: Shao
authors: Parsin
authors: P
authors: Hajireza
authors: Alexander
authors: A
authors: Forbrich
authors: Roger J
authors: RJ
authors: Zemp
mesh_terms: D000818:Animals; D003199:Computer Systems; D004423:Ear / Q000098:blood supply; D006439:Hemodynamics; D053844:Lasers, Solid-State; D051379:Mice; D016513:Mice, SCID; D033401:Microscopy, Acoustic / Q000295:instrumentation / Q000379:methods*; D055806:Microvessels / Q000033:anatomy & histology / Q000502:physiology; D055096:Optical Devices; D055620:Optical Phenomena; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*