title: Adipose derived mesenchymal stem cells - their osteogenicity and osteoblast in vitro mineralization on titanium granule carriers.
abstract: OBJECTIVE
  Adipose derived mesenchymal stem cells (ADMSCs) may be osteogenic, may generate neoangiogenisis and may be progenitors for differentiated osteoblast mineralization. Titanium granules may be suitable as carriers for these cells. The aim was to demonstrate the osteogenic potential of ADMSCs and the effect of porous non-oxidized (Ti) and oxidized titanium (TiO2) granules as carriers for ADMSCs mineralization in vitro.
  
  
  METHODS
  ADMSCs were isolated, cultivated in osteoblast medium and evaluated for alkaline phosphatase (ALP) assay, RNA isolation, and ALP staining. Osteoblast in vitro mineralization cells without granules or seeded on Ti or TiO2 granules were evaluated for Alizarin Red assay and RNA isolation for later gene expressing.
  
  
  RESULTS
  ADMSCs express osteoblastic lineage genes, CBFA-1 and stain strongly for ALP. Mineralization was significantly higher for cells seeded on TiO2 than on Ti granules or pure cells. Expression of ALPL and RUNX2 was significantly higher for cells seeded on TiO2 granules and expression of COL1α1 for pure cells was significantly higher than for cells seeded on granules.
  
  
  CONCLUSIONS
  ADMSCs have osteogenic potential. Mineralization was significantly high when cells were seeded on TiO2 granules. TiO2 granules may be used as carriers for adipose derived mesenchymal osteoblastic cells from laboratory bench to the patient.
authors: Institute of Odontology, Department of Oral and Maxillofacial Surgery, Faculty of Health Sciences, University of Copenhagen, Nørre Allé 20, 2200 Copenhagen, Denmark.
authors: Morten
authors: M
authors: Dahl
authors: Susanne
authors: S
authors: Syberg
authors: Niklas Rye
authors: NR
authors: Jørgensen
authors: Else Marie
authors: EM
authors: Pinholt
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000880:Anthraquinones; D001672:Biocompatible Materials / Q000737:chemistry; D002113:Calcification, Physiologic / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D024042:Collagen Type I / Q000032:analysis; D000090602:Collagen Type I, alpha 1 Chain; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D018919:Neovascularization, Physiologic / Q000502:physiology; D010006:Osteoblasts / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D001672:Biocompatible Materials; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; C009495:titanium dioxide; C010078:alizarin; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Adipose mesenchymal stem cells; Drug carriers; Mineralization; Tissue engineering; Titanium