title: Heat-treated emulsions with cross-linking bovine serum albumin interfacial films and different dextran surfaces: effect of paclitaxel delivery.
abstract: In this study, a type of biocompatible and biodegradable oil-in-water emulsion for hydrophobic drug delivery was evaluated in vitro and in vivo. Bovine serum albumin (BSA)-dextran conjugates with different dextran molecular weights and different conjugation degrees were used as the emulsifier and stabilizer. Paclitaxel (PTX), a hydrophobic antitumor drug, was effectively loaded inside the oil droplets via high-pressure homogenization. The emulsions were heated at 90°C for 1 h to eliminate the anaphylaxis of BSA. By virtue of the cross-linked BSA films at the oil-water interfaces produced by the heat treatment and the hydrophilic dextran surfaces, the emulsions are stable in blood serum, as well as stable against long-term storage. In vitro cytotoxicity study verifies that the unloaded emulsions are biocompatible and the PTX-loaded emulsions have similar antitumor activity as PTX solution. In vivo investigation of murine ascites hepatoma H22-tumor-bearing mice demonstrates that the PTX-loaded emulsion with shorter and denser dextran surface has better tumor inhibition and survivability efficacy than the commercial PTX injection.
authors: State Key Laboratory of Molecular Engineering of Polymer, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors: Jianing
authors: J
authors: Qi
authors: Chong
authors: C
authors: Huang
authors: Fen
authors: F
authors: He
authors: Ping
authors: P
authors: Yao
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007624:KB Cells; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D017239:Paclitaxel / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003911:Dextrans; D004337:Drug Carriers; D004655:Emulsions; D012710:Serum Albumin, Bovine; D017239:Paclitaxel