title: Cystic fibrosis CFBE41o- cells contain TLR1 SNP I602S and fail to respond to Mycobacterium abscessus.
abstract: BACKGROUND
  Mycobacterium abscessus causes lung infection in patients with cystic fibrosis. M. abscessus stimulates the host innate immune response via TLR2 on respiratory epithelial cells. Signaling through TLR2 requires the formation of TLR2/TLR1 heterodimers on the cell surface.
  
  
  METHODS
  The ability of M. abscessus to stimulate the innate immune response of cystic fibrosis CFBE41o- respiratory epithelial cells was measured as expression of HβD2 by RT PCR, and release of IL-8 by ELISA. Genotyping of CFBE41o- TLR polymorphisms was carried out.
  
  
  RESULTS
  CFBE41o- cells are hyporesponsive to M. abscessus. They are homozygous for the TLR1 SNP I602S which has been demonstrated to cause diminished cellular responses to TLR2 agonists.
  
  
  CONCLUSIONS
  Homozygosity for I602S is prevalent in Western Europeans and North American Caucasians, the same demographic in which the ΔF508 mutation is present. This SNP may play a role in the pathogenesis of M. abscessus lung infection in patients with cystic fibrosis.
authors: The University of New Mexico School of Medicine, Center for Global Health, Albuquerque, NM 87131, USA.
authors: Prakasha
authors: P
authors: Kempaiah
authors: Lisa B
authors: LB
authors: Davidson
authors: Douglas J
authors: DJ
authors: Perkins
authors: Thomas F
authors: TF
authors: Byrd
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000235:genetics* / Q000382:microbiology; D004847:Epithelial Cells; D006801:Humans; D007113:Immunity, Innate; D009165:Mycobacterium Infections, Nontuberculous / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D051194:Toll-Like Receptor 1 / Q000235:genetics*
chemical_list: D051194:Toll-Like Receptor 1
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