title: Self-assembly of quantum dots/denatured BSA-oligonucleotides bioconjugate and its application on aptameric gold nanoparticles-based biosensor for the determination of rHuEPO-α.
abstract: In this paper, denatured bovine serum albumin (dBSA) has been developed as a useful scaffold for appending multiple oligonucleotides (ODNs), and the resulted novel dBSA-ODNs covalent hybrid can be self-assembled to the surface of CdTe quantum dots (QDs) via complexation with unoccupied coordination metal sites on QDs' surface. The formed QDs/dBSA-ODNs bioconjugate could keep a long-term stability in aqueous solution while maintaining its fluorescence quantum yield and spectral properties. Furthermore, the QDs/dBSA-ODNs also can be readily hybridized with an anti-rHuEPO-α aptamers linked AuNPs (Apt-AuNPs) to form a fluorescent resonance energy transfer-based nano-biosensing system for highly sensitive and selective detection of rHuEPO-α protein lowered to 53.6 pM in a rapid, simple manner.
authors: Beijing Institute of Pharmacology and Toxicology, No. 27, Taiping Road, Beijing 100850, China.
authors: Jiefang
authors: J
authors: Sun
authors: Lei
authors: L
authors: Guo
authors: Hua
authors: H
authors: Xu
authors: Jijun
authors: J
authors: Tang
authors: Jianwei
authors: J
authors: Xie
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004921:Erythropoietin / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011489:Protein Denaturation; D045663:Quantum Dots; D011994:Recombinant Proteins / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; D011994:Recombinant Proteins; D004921:Erythropoietin; D012710:Serum Albumin, Bovine; D006046:Gold