title: Nanodiscs as a new tool to examine lipid-protein interactions.
abstract: Nanodiscs are self-assembled discoidal fragments of lipid bilayers 8-16 nm in diameter, stabilized in solution by two amphipathic helical scaffold proteins. As stable and highly soluble membrane mimetics with controlled lipid composition and ability to add affinity tags to the scaffold protein, nanodiscs represent an attractive model system for solubilization, isolation, purification, and biophysical and biochemical studies of membrane proteins. In this chapter we overview various approaches to structural and functional studies of different classes of integral membrane proteins such as ion channels, transporters, GPCR and other receptors, membrane enzymes, and blood coagulation cascade proteins which have been incorporated into nanodiscs. We outline the advantages provided by homogeneity, ability to control oligomerization state of the target protein and lipid composition of the bilayer. Special attention is paid to the opportunities afforded by nanodisc system for the detailed studies of the role of different lipid properties and protein-lipid interactions in the functional behavior of membrane proteins.
authors: Departments of Biochemistry and Cell and Developmental Biology, University of Illinois, Urbana, IL, USA.
authors: Mary A
authors: MA
authors: Schuler
authors: Ilia G
authors: IG
authors: Denisov
authors: Stephen G
authors: SG
authors: Sligar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism*; D050356:Lipid Metabolism; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011485:Protein Binding
chemical_list: D008051:Lipid Bilayers
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