title: Growth kinetics and immune response of chimeric foot-and-mouth disease virus serotype 'O' produced through replication competent mini genome of serotype Asia 1, 63/72, in BHK cell lines.
abstract: Regular vaccinations with potent vaccine, in endemic countries and vaccination to live in non-endemic countries are the methods available to control foot-and-mouth disease. Selection of candidate vaccine strain is not only cumbersome but the candidate should grow well for high potency vaccine preparation. Alternative strategy is to generate an infectious cDNA of a cell culture-adapted virus and use the replicon for development of tailor-made vaccines. We produced a chimeric 'O' virus in the backbone of Asia 1 and studied its characteristics. The chimeric virus showed high infectivity titre (>10(10)) in BHK 21 cell lines, revealed small plaque morphology and there was no cross reactivity with antiserum against Asia 1. The virus multiplies rapidly and reaches peak at 12h post infection. The vaccine prepared with this virus elicited high antibody titres.
authors: Indian Veterinary Research Institute, Hebbal, Bangalore 560024, India.
authors: Rahul
authors: R
authors: Joshi
authors: S
authors: S
authors: Chandrasekar
authors: Soumya
authors: S
authors: Paul
authors: Ashok Kumar
authors: AK
authors: Chokkalingam
authors: T
authors: T
authors: Saravanan
authors: Kakoli
authors: K
authors: Ahmad
authors: H J
authors: HJ
authors: Dechamma
authors: G R
authors: GR
authors: Reddy
authors: S M
authors: SM
authors: Gopinath
authors: D N
authors: DN
authors: Das
authors: M S
authors: MS
authors: Shaila
authors: G N
authors: GN
authors: Bharath
authors: V V S
authors: VV
authors: Suryanarayana
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D006224:Cricetinae; D003429:Cross Reactions; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000254:growth & development* / Q000276:immunology*; D011995:Recombination, Genetic; D044967:Serum / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines