title: Biodegradable and redox-responsive chitosan/poly(L-aspartic acid) submicron capsules for transmucosal delivery of proteins and peptides.
abstract: The development of peptides and proteins is hampered by their rapid clearance in liver and other body tissues by proteolytic enzymes, so these drugs are difficult to administer except for the injection. Here, we designed and fabricated a novel biodegradable and redox-responsive submicron capsules through the layer-by-layer technique with poly(L-aspartic acid) and chitosan for transmucosal delivery of proteins and peptides. TEM graphs reveal that the intact submicron capsules were obtained and the shell of submicron capsules was about 40 nm. The mucoadhesion test indicates that the adsorption amount of the mucin could achieve up to 96.2 μg per 2 mg. The cell viability test shows that all types of submicron capsules had good cytocompatibility and the cell viability was above 90 %. As a drug model, the insulin could be loaded in the submicron capsules, and the loading efficiency was about 5 %. The release amount of insulin could be regulated by the levels of GSH. Therefore, the mucoadhesive submicron capsules as vehicles have a potential for the mucosal delivery (e.g. nasal and buccal) of therapeutic peptide and protein drugs.
authors: The Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin, China.
authors: C
authors: C
authors: Zheng
authors: X G
authors: XG
authors: Zhang
authors: L
authors: L
authors: Sun
authors: Z P
authors: ZP
authors: Zhang
authors: C X
authors: CX
authors: Li
mesh_terms: D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D009092:Mucous Membrane; D010084:Oxidation-Reduction; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D011506:Proteins / Q000008:administration & dosage*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010455:Peptides; D011506:Proteins; C017645:polyaspartate; D048271:Chitosan
references: Drug Dev Ind Pharm. 2005 Oct;31(9):885-93
references: 16306000
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Biomaterials. 2009 Oct;30(29):5691-700
references: 19615735
references: Pharm Res. 1996 Nov;13(11):1686-92
references: 8956335
references: Small. 2009 Apr;5(4):444-8
references: 19173351
references: Langmuir. 2008 Dec 2;24(23):13723-9
references: 18855487
references: Biomaterials. 2000 Dec;21(24):2589-98
references: 11071608
references: Acta Biomater. 2010 Jan;6(1):210-7
references: 19531385
references: J Colloid Interface Sci. 2010 May 15;345(2):241-7
references: 20227712
references: Biomacromolecules. 2006 Jan;7(1):27-30
references: 16398494
references: J Control Release. 2011 May 30;152(1):2-12
references: 21295087
references: Biomaterials. 2012 Feb;33(5):1607-17
references: 22118820
references: Biomaterials. 2012 Jan;33(2):614-23
references: 22014947
references: J Biomed Mater Res B Appl Biomater. 2009 Jan;88(1):150-61
references: 18618466
references: Eur J Pharm Sci. 2009 May 12;37(2):83-8
references: 19429414
references: J Control Release. 2011 Oct 10;155(1):54-9
references: 21185892
references: Small. 2009 Nov;5(22):2601-10
references: 19771568
references: Carbohydr Res. 2009 Jul 6;344(10):1197-204
references: 19508912
references: J Chem Inf Model. 2005 Nov-Dec;45(6):1589-92
references: 16309258