title: Fabrication, properties and bioapplications of cellulose/collagen hydrolysate composite films.
abstract: In this work, a series of cellulose/collagen hydrolysate (RC/CH) films were prepared in NaOH/urea aqueous solution via a simple, low-cost and green pathway. To overcome the disadvantages (brittleness, poor water resistance) of CH as biomaterials, CH was combined with regenerated cellulose (RC) film to construct RC/CH composite materials, leading to vast improvement of the water resistance of CH. Crosslinking with genipin further improved the mechanical properties of the RC/CH films in the wet state. Their structure and properties were characterized by elemental analysis, Fourier transform infra-red (FT-IR) spectra, ultraviolet-visible (UV-vis), scanning electron microscopy (SEM), amino acid analysis, tensile testing, cell adhesion and toxicity tests. The mechanical properties and water resistance of the crosslinked RC/CH films were significantly improved, which made collagen hydrolysate as biomaterial could be used at wet state. Moreover, the RC/CH films exhibited good biocompatibility by proliferation of COS7 cells on the surface, supporting cell adhesion and growth.
authors: Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors: Ying
authors: Y
authors: Pei
authors: Juan
authors: J
authors: Yang
authors: Pan
authors: P
authors: Liu
authors: Min
authors: M
authors: Xu
authors: Xianzheng
authors: X
authors: Zhang
authors: Lina
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D019556:COS Cells; D002482:Cellulose / Q000737:chemistry*; D002522:Chlorocebus aethiops; D003094:Collagen / Q000737:chemistry*; D006868:Hydrolysis; D055595:Mechanical Phenomena; D012972:Sodium Hydroxide / Q000737:chemistry; D014508:Urea / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D014867:Water; D012972:Sodium Hydroxide; D014508:Urea; D002482:Cellulose; D003094:Collagen