title: Carbonic anhydrase type IX expression in lobular endocervical glandular hyperplasia and gastric-type adenocarcinoma of the uterine cervix.
abstract: The significance of carbonic anhydrase (CA-IX) expression in endocervical glandular lesions was determined to assess the utility of CA-IX immunohistochemistry for the identification of gastric-type endocervical adenocarcinoma and its related lesions. In this study, lobular endocervical glandular hyperplasia (LEGH) (n=5), atypical LEGH (ALEGH) (n=6), and endocervical adenocarcinomas, including usual-type (UEA) (n=6) and gastric-type (GAS) (n=7), were examined by immunohistochemistry for CA-IX expression. The proportion of positive staining and intensity was evaluated as between 0 and 3+. All 13 UEAs and GASs were positive for CA-IX, with 9 cases showing 3+ staining, and 8 cases showing positive areas exceeding 50%. All 11 LEGHs were positive for CA-IX, with all cases showing 50% or more positive areas, although only one case was 3+ positive. Five of 6 ALEGHs were positive for CA-IX, with 4 cases showing 3+ positivity in 100% of the lesion. Non-neoplastic glands were negative for CA-IX, although reserve cell populations and tubal metaplasias were positive for CA-IX. Although CA-IX appears to be involved in tumor progression and may be diagnostically useful in cases of endocervical adenocarcinoma and its precursors, LEGH can be a pitfall, and staining results should be combined with morphology.
authors: Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto, Japan. mika@kuhp.kyoto-u.ac.jp
authors: Yoshiki
authors: Y
authors: Mikami
authors: Sachiko
authors: S
authors: Minamiguchi
authors: Norihiro
authors: N
authors: Teramoto
authors: Michikazu
authors: M
authors: Nagura
authors: Hironori
authors: H
authors: Haga
authors: Ikuo
authors: I
authors: Konishi
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000951:Antigens, Neoplasm / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000032:analysis / Q000096:biosynthesis*; D002584:Cervix Uteri / Q000201:enzymology / Q000473:pathology; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000201:enzymology / Q000473:pathology; D007150:Immunohistochemistry; D011230:Precancerous Conditions / Q000201:enzymology / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases