title: [Orthogonal test for preparing genipin with enzymolyzed geniposide].
abstract: OBJECTIVE
  To optimize the process for preparing genipin with enzymolyzed geniposide by an orthogonal experiment.
  
  
  METHODS
  The optimal enzymolysis process was selected by an orthogonal experiment, with the concentration of geniposide as the index as well as enzyme quantity, pH of enzymolysis solution, enzymolysis time and enzymolysis temperature as considerations.
  
  
  RESULTS
  The optimal hydrolysis conditions were as follow: rough genipin samples at the concentration of 40 g x L(-1) were selected and shook on a table concentrator at a speed of 100 r x min(-1), added with beta-glucosidase-geniposide 1 : 1 (weight proportion), with pH of enzymolysis solution at 4.5, hydrolyzation temperature at 50 degrees C, the conversion rate of genipin could reach 85.8%.
  
  
  CONCLUSIONS
  The process is so stable and feasible that it can provide theoretical basis for the preparation of genipin with enzymolyzed geniposide.
authors: Jiangsu Kanion Pharmaceutical Co., Ltd, Lianyungang 222001, China.
authors: Xiao-Song
authors: XS
authors: Niu
authors: Zhao-Qing
authors: ZQ
authors: Meng
authors: Ning-Ho
authors: NH
authors: Cheng
authors: Jing-Yan
authors: JY
authors: Zhu
authors: Gang
authors: G
authors: Ding
authors: Yu-An
authors: YA
authors: Bi
authors: Zhen-Zhong
authors: ZZ
authors: Wang
authors: Wei
authors: W
authors: Xiao
mesh_terms: D006868:Hydrolysis; D039823:Iridoids / Q000737:chemistry*; D013678:Technology, Pharmaceutical
chemical_list: D039823:Iridoids; C007835:geniposide; C007834:genipin