title: Characterization of novel HMW-GS in two diploid species of Eremopyrum.
abstract: Three HMW-GS and the respective ORFs from diploid species Eremopyrum distans and Eremopyrum triticeum were characterized. Compared to homologous proteins, they showed novel modifications in all domains. In the N-terminals, the y subunit from Er. triticeum (Xey) had 98 aa residues. A short G/IIFWGTS peptide deletion was responsible for the reduced number of aa residues. The end peptide in the y subunit from Er. distans (Fy) was IPTLLR. This unique structure was involved in a replacement between x types with IPA/TLLK/R and y types with R/TSSQTVQ. Both y subunits share the same short peptide LAAQLPAMCRL as x types in the C-terminals. Phylogenic relationships among orthologous genes from Triticeae species revealed that Fy and Xey were neither purely x type nor purely y type based on the N and C terminal residues. Divergence times indicated that Glu-Xe1 and Glu-F1 were separated from each other and that Glu-Xe1 separated from orthologous loci of wild wheat relatives earlier than Glu-F1. Based on the divergence times among Glu-F1, Glu-Xe1, Glu-O1, Glu-St1, and Glu-Ta1, it is possible that genome F separation from O, St, and Ta in species of Henrardia persica, Pseudoroegneria stipifolia, and Taeniatherum crinitum was more recent than the separation of F and Xe.
authors: Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China.
authors: Shoufen
authors: S
authors: Dai
authors: Zongjun
authors: Z
authors: Pu
authors: Dengcai
authors: D
authors: Liu
authors: Yuming
authors: Y
authors: Wei
authors: Youliang
authors: Y
authors: Zheng
authors: Xinkun
authors: X
authors: Hu
authors: Zehong
authors: Z
authors: Yan
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D004171:Diploidy; D004591:Electrophoresis, Polyacrylamide Gel; D019143:Evolution, Molecular; D017353:Gene Deletion; D056426:Genetic Loci; D018745:Genome, Plant; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D006109:Poaceae / Q000737:chemistry* / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005983:Glutens; C037302:glutenin