title: [Alteration of transport activity of proton pumps in coleoptile cells during early development stages of maize seedlings].
abstract: Comparative analysis of the transport activity of proton pumps (plasmalemma H+-ATPase, vacuolar H+-ATPase, and vacuolar H+-pyrophosphatase) in the membrane preparations obtained from coleoptile cells ofetiolated maize seedlings (Zea mays L.) was carried out. The highest level ofvacuolar pyrophosphatase activity was observed during the early development of coleoptile cells under growth intensification through the elongation. The role of ATPase pumps of tonoplast and plasmalemma in the transport of hydrogen ions increases during further development. The plasmalemma activity in this process is higher. When the growth stops, the activity of proton pumps becomes significantly lower. Nevertheless, their substrate specificity and sensitivity to proton pump inhibitors do not change, which can be an evidence of physiological significance of pumps in the maintenance of cell homeostasis.
authors: M F
authors: MF
authors: Shishova
authors: O V
authors: OV
authors: Tankeliun
authors: E L
authors: EL
authors: Rudashevskaia
authors: V V
authors: VV
authors: Emel'ianov
authors: N V
authors: NV
authors: Shakhova
authors: A A
authors: AA
authors: Kirpichnikova
mesh_terms: D000165:Acridine Orange; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D001693:Biological Transport, Active / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D018548:Cotyledon / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D003911:Dextrans; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D006706:Homeostasis / Q000502:physiology; D043564:Inorganic Pyrophosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054328:Proton Pump Inhibitors / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014617:Vacuoles / Q000187:drug effects / Q000201:enzymology; D014634:Valinomycin / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; D054328:Proton Pump Inhibitors; C015219:fluorescein isothiocyanate dextran; D014634:Valinomycin; D000255:Adenosine Triphosphate; D025262:Vacuolar Proton-Translocating ATPases; D043564:Inorganic Pyrophosphatase; D006180:Proton-Translocating ATPases; D000165:Acridine Orange; D016650:Fluorescein-5-isothiocyanate