title: Method for the validation of immunohistochemical staining using SCID mouse xenografts: expression of CD40 and CD154 in human non-small cell lung cancer.
abstract: This report proposes a concept for the standardization of immunohistochemical             evaluation. Immunohistochemical staining has several problems associated with             the sensitivity of the technical process and standardization of the assessment             of potent staining. We provided data focusing on this concept through immunostaining             for CD154 in non-small cell lung cancer (NSCLC). We used two types of anti-CD154             antibody as primary antibodies in immunohistochemical staining, as previously             reported. Western blot analysis confirmed strong CD154 expression in the cultured             cell line PC10, but not in LK2. We also assessed CD154 expression in SCID mouse             xenografts of these cell lines. SCID xenograft data on western blot analysis were             consistent with those of cultured cell lines. These xenografts could thus be used             as positive or negative tissue controls for CD154 immunostaining. Primary antibodies             should therefore be confirmed as recognizing target lesions, while control tissue             specimens should be objectively confirmed as having target products using another             experimental method. Our method would allow results to be unified at more than             one laboratory and could act as an objective control assessment method in immunohistochemistry.
authors: Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, and Department of Thoracic Surgery, Sapporo-Minamisanjo Hospital, Sapporo, Hokkaido 060-8638, Japan. keidaiishikawa@hotmail.com
authors: Keidai
authors: K
authors: Ishikawa
authors: Masaki
authors: M
authors: Miyamoto
authors: Tatsuya
authors: T
authors: Yoshioka
authors: Masatoshi
authors: M
authors: Kadoya
authors: Li
authors: L
authors: Li
authors: Roshan
authors: R
authors: Mishra
authors: Kazuomi
authors: K
authors: Ichinokawa
authors: Yasuhito
authors: Y
authors: Shoji
authors: Yoshiyuki
authors: Y
authors: Matsumura
authors: Yasuhiro
authors: Y
authors: Hida
authors: Kichizo
authors: K
authors: Kaga
authors: Tatsuya
authors: T
authors: Kato
authors: Mitsuhito
authors: M
authors: Kaji
authors: Toshiro
authors: T
authors: Ohbuchi
authors: Tomoo
authors: T
authors: Itoh
authors: Hirotoshi
authors: H
authors: Dosaka-Akita
authors: Yoshiro
authors: Y
authors: Matsui
authors: Satoshi
authors: S
authors: Hirano
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000276:immunology; D019013:CD40 Antigens / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D023201:CD40 Ligand / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D016513:Mice, SCID; D013194:Staining and Labeling; D023041:Xenograft Model Antitumor Assays
chemical_list: D000888:Antibodies, Anti-Idiotypic; D019013:CD40 Antigens; D023201:CD40 Ligand
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