title: Polydiacetylene liposome microarray toward influenza a virus detection: effect of target size on turn-on signaling.
abstract: Target size effect on the sensory signaling intensity of polydiacetylene (PDA) liposome microarrays was systematically investigated. Influenza A virus M1 peptide and M1 antibody were selected as a probe-target pair. While red fluorescence from the PDA liposome microarrays was observed when the larger M1 antibody was used as a target, when the same M1 antibody was used as a probe to detect the smaller M1 peptide sensory signal did not appear. The results reveal that the intensity of the PDA sensory signal is mainly related to the steric repulsion between probe-target complexes not the strength of the probe-target binding force. Based on this finding, we devised a PDA sensory system that directly detects influenza A whole virus as a larger target, and confirmed the target size effect on the signaling efficiency of PDA.
authors: Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109-2136, USA.
authors: Sungbaek
authors: S
authors: Seo
authors: Jiseok
authors: J
authors: Lee
authors: Eun-Jin
authors: EJ
authors: Choi
authors: Eun-Ju
authors: EJ
authors: Kim
authors: Jae-Young
authors: JY
authors: Song
authors: Jinsang
authors: J
authors: Kim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D009980:Influenza A virus / Q000737:chemistry* / Q000235:genetics*; D008081:Liposomes / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D015347:RNA Probes / Q000737:chemistry; D012367:RNA, Viral / Q000032:analysis
chemical_list: D008081:Liposomes; D000078789:Polyacetylene Polymer; D011108:Polymers; D015347:RNA Probes; D012367:RNA, Viral; D053279:Polyynes; C082361:polydiacetylene; D004134:Dimyristoylphosphatidylcholine