title: Intra-femoral injection of human mesenchymal stem cells.
abstract: In vivo transplantation of putative populations of hematopoietic stem cells (HSC) and assessment of their engraftment is considered the golden standard to assess their quality and degree of stemness. Transplantation is usually carried out by intravenous injection in murine models and assessment of engraftment is performed by monitoring the number and type of mature blood cells produced by the donor cells in time. In contrast intravenous injection of mesenchymal stem cells (MSC), the multipotent stem cells present in bone marrow and capable of differentiating to osteoblasts, chondrocytes and adipocytes, has not been successful. This is due to limited or absent engraftment levels. Here, we describe the use of intra-femoral injection as an improved method to assess MSC engraftment to bone and bone marrow and their quality.
authors: Department of Human Metabolism, Mellanby Centre for Bone Research, University of Sheffield, Sheffield, UK.
authors: Sindhu T
authors: ST
authors: Mohanty
authors: Ilaria
authors: I
authors: Bellantuono
mesh_terms: D001853:Bone Marrow / Q000254:growth & development / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005269:Femur / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins