title: Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay.
abstract: Microtubule (MT) based intraneuronal transport deficiency is directly linked to neurodegeneration. Hence, the development of a reliable and sensitive in vitro approach permitting efficient analysis of MT-based transport is essential for our understanding of the underlying molecular mechanisms that may lead to novel therapeutic approaches for treating neurodegenerative diseases. Here, based on previously developed reconstructed MT-kinesin assay, we propose its "suspended" modification that shows higher sensitivity and lower experimental variability.
authors: Center for International Research on Micronano Mechatronic, Institute of Industrial Science, The University of Tokyo, 4-6-1, Komaba, Tokyo 153-8505, Japan. mctarhan@iis.u-tokyo.ac.jp
authors: Mehmet C
authors: MC
authors: Tarhan
authors: Yslam
authors: Y
authors: Orazov
authors: Ryuji
authors: R
authors: Yokokawa
authors: Stanislav L
authors: SL
authors: Karsten
authors: Hiroyuki
authors: H
authors: Fujita
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D057075:Enzyme Assays / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D019636:Neurodegenerative Diseases / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D016547:Kinesins