title: Protective effect of l-theanine on chronic restraint stress-induced cognitive impairments in mice.
abstract: The present work was aimed to study the protective effect of l-theanine on chronic restraint stress (CRS)-induced cognitive impairments in mice. The stress was produced by restraining the animals in well-ventilated polypropylene tubes (3.2 cm in diameter ×10.5 cm in length) for 8h once daily for 21 consecutive days. L-theanine (2 and 4 mg/kg) was administered 30 min before the animals subjected to acute immobilized stress. At week 4, mice were subjected to Morris water maze and step-through tests to measure the cognitive function followed by oxidative parameters and corticosterone as well as catecholamines (norepinephrine and dopamine) subsequently. Our results showed that the cognitive performances in CRS group were markedly deteriorated, accompanied by noticeable alterations in oxidative parameters and catecholamine levels in the hippocampus and the cerebral cortex as well as corticosterone and catecholamine levels in the serum. However, not only did l-theanine treatment exhibit a reversal of the cognitive impairments and oxidative damage induced by CRS, but also reversed the abnormal level of corticosterone in the serum as well as the abnormal levels of catecholamines in the brain and the serum. This study indicated the protective effect of l-theanine against CRS-induced cognitive impairments in mice.
authors: Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. xuezhetian@163.com
authors: Xia
authors: X
authors: Tian
authors: Lingyan
authors: L
authors: Sun
authors: Lingshan
authors: L
authors: Gou
authors: Xin
authors: X
authors: Ling
authors: Yan
authors: Y
authors: Feng
authors: Ling
authors: L
authors: Wang
authors: Xiaoxing
authors: X
authors: Yin
authors: Yi
authors: Y
authors: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003072:Cognition Disorders / Q000188:drug therapy* / Q000209:etiology*; D003345:Corticosterone / Q000378:metabolism; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005971:Glutamates / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009638:Norepinephrine / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011597:Psychomotor Performance / Q000187:drug effects; D012149:Restraint, Physical / Q000009:adverse effects*; D013315:Stress, Psychological / Q000150:complications* / Q000209:etiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D005971:Glutamates; C026166:theanine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D004298:Dopamine; D003345:Corticosterone; D009638:Norepinephrine