title: Goat mammary gland expression of Cecropin B to inhibit bacterial pathogens causing mastitis.
abstract: The antibacterial peptide Cecropin B (CB), isolated from the giant silk moth, has been shown to effectively eliminate bacteria. In this study, the effects of transgenic CB on dairy goat mammary epithelial cells (DGMECs) and dairy goat mammary gland were investigated. The DNA of CB from silkworm was amplified by reverse transcription PCR (RT-PCR) and then fused to the eukaryotic expression vector pECFP-C1. The recombinant plasmid pECFP-Cecropin B (pECFP-CB) was used for the transfection of DGMECs, and the expression of transgenic CB and the antibacterial activity of it were confirmed by western blot and agar diffusion reaction respectively. The stable DGMEC line transfected by pECFP-CB was obtained by screening with G418. In vivo experiment, pECFP-CB was injected into dairy goat mammary gland, and also the expression and antibacterial activity of transgenic CB were confirmed. Results of this study: transgenic CB can be expressed in DGMECs and dairy goat mammary gland, and inhibit the mastitis caused by Staphylococcus aureus.
authors: Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin, China.
authors: Chao-chao
authors: CC
authors: Luo
authors: De-yun
authors: DY
authors: Yin
authors: Xue-jun
authors: XJ
authors: Gao
authors: Qing-zhang
authors: QZ
authors: Li
authors: Li
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology; D006041:Goats / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D008413:Mastitis / Q000382:microbiology / Q000517:prevention & control*; D008892:Milk / Q000737:chemistry / Q000382:microbiology; D010957:Plasmids; D013203:Staphylococcal Infections / Q000517:prevention & control / Q000662:veterinary; D013211:Staphylococcus aureus; D014162:Transfection
chemical_list: D000900:Anti-Bacterial Agents; D019476:Insect Proteins; C031162:cecropin B protein, Insecta