title: A simple medium enables bovine embryos to be held for seven days at 4°C.
abstract: Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze-thawing causes significant damage. The strict regulation of transportation of LN(2) containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN(2)-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days.
authors: Research and Development Group, Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido 080-1407, Japan. ideta-atsushi@zennoh.or.jp
authors: Atsushi
authors: A
authors: Ideta
authors: Yoshito
authors: Y
authors: Aoyagi
authors: Kanami
authors: K
authors: Tsuchiya
authors: Tatsuro
authors: T
authors: Kamijima
authors: Yoshiyuki
authors: Y
authors: Nishimiya
authors: Sakae
authors: S
authors: Tsuda
mesh_terms: D000818:Animals; D001723:Birth Rate; D002417:Cattle; D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D011247:Pregnancy; D018873:Pregnancy Rate; D013997:Time Factors
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