title: Directed evolution of a synthetic RNA-protein module to create a new translational switch.
abstract: A synthetic RNP-binding module was developed to produce an alternative translational switch: an in vitro selection experiment targeting a derivative of L7Ae protein (L7KK) identified a new H23 RNA aptamer that binds tightly to both L7KK and L7Ae. The switch serves as a translational OFF switch for constructing a NOR gate.
authors: Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Kyoto, 606-8502, Japan.
authors: Tomoaki
authors: T
authors: Hara
authors: Hirohide
authors: H
authors: Saito
authors: Tan
authors: T
authors: Inoue
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D024202:Electrophoretic Mobility Shift Assay; D005818:Genetic Engineering; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D014176:Protein Biosynthesis; D019642:RNA, Archaeal / Q000235:genetics / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D019843:Archaeal Proteins; D019642:RNA, Archaeal