title: Sulfonation and anticoagulant activity of fungal exocellular β-(1→6)-D-glucan (lasiodiplodan).
abstract: An exocellular β-(1→6)-D-glucan (lasiodiplodan) produced by a strain of Lasiodiplodia theobromae (MMLR) grown on sucrose was derivatized by sulfonation to promote anticoagulant activity. The structural features of the sulfonated β-(1→6)-D-glucan were investigated by UV-vis, FT-IR and (13)C NMR spectroscopy, and the anticoagulant activity was investigated by the classical coagulation assays APTT, PT and TT using heparin as standard. The content of sulfur and degree of substitution of the sulfonated glucan was 11.73% and 0.95, respectively. UV spectroscopy showed a band at 261 nm due to the unsaturated bond formed in the sulfonation reaction. Results of FT-IR and (13)C NMR indicated that sulfonyl groups were inserted on the polysaccharide. The sulfonated β-(1→6)-D-glucan presented anticoagulant activity as demonstrated by the increase in dose dependence of APTT and TT, and these actions most likely occurred because of the inserted sulfonate groups on the polysaccharide. The lasiodiplodan did not inhibit the coagulation tests.
authors: Dept° de Física, Química e Biologia, Universidade Estadual Paulista - UNESP, CEP 19060-900, Presidente Prudente, São Paulo, Brazil.
authors: Ana Flora D
authors: AF
authors: Vasconcelos
authors: Robert F H
authors: RF
authors: Dekker
authors: Aneli M
authors: AM
authors: Barbosa
authors: Elaine R
authors: ER
authors: Carbonero
authors: Joana L M
authors: JL
authors: Silveira
authors: Bianca
authors: B
authors: Glauser
authors: Mariana Sá
authors: MS
authors: Pereira
authors: Maria de Lourdes
authors: Mde L
authors: Corradi da Silva
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D000991:Antithrombins / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry* / Q000254:growth & development; D001780:Blood Coagulation Tests; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D012995:Solubility; D013395:Sucrose / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry*; D013917:Thrombin / Q000037:antagonists & inhibitors; D014867:Water / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000925:Anticoagulants; D000991:Antithrombins; D013451:Sulfonic Acids; D047071:beta-Glucans; D014867:Water; C064197:beta-1,6-glucan; D013395:Sucrose; D006493:Heparin; D013917:Thrombin