title: Genetic and physiological characterization of two clusters of quantitative trait Loci associated with seed dormancy and plant height in rice.
abstract: Seed dormancy and plant height have been well-studied in plant genetics, but their relatedness and shared regulatory mechanisms in natural variants remain unclear. The introgression of chromosomal segments from weedy into cultivated rice (Oryza sativa) prompted the detection of two clusters (qSD1-2/qPH1 and qSD7-2/qPH7) of quantitative trait loci both associated with seed dormancy and plant height. Together, these two clusters accounted for >96% of the variances for plant height and ~71% of the variances for germination rate in an isogenic background across two environments. On the initial introgression segments, qSD1-2/qPH1 was dissected genetically from OsVp1 for vivipary and qSD7-2/qPH7 separated from Sdr4 for seed dormancy. The narrowed qSD1-2/qPH1 region encompasses the semidwarf1 (sd1) locus for gibberellin (GA) biosynthesis. The qSD1-2/qPH1 allele from the cultivar reduced germination and stem elongation and the mutant effects were recovered by exogenous GA, suggesting that sd1 is a candidate gene of the cluster. In contrast, the effect-reducing allele at qSD7-2/qPH7 was derived from the weedy line; this allele was GA-insensitive and blocked GA responses of qSD1-2/qPH1, including the transcription of a GA-inducible α-amylase gene in imbibed endosperm, suggesting that qSD7-2/qPH7 may work downstream from qSD1-2/qPH1 in GA signaling. Thus, this research established the seed dormancy-plant height association that is likely mediated by GA biosynthesis and signaling pathways in natural populations. The detected association contributed to weed mimicry for the plant stature in the agro-ecosystem dominated by semidwarf cultivars and revealed the potential benefit of semidwarf genes in resistance to preharvest sprouting.
authors: Plant Science Department, South Dakota State University, Brookings, South Dakota 57007, USA.
authors: Heng
authors: H
authors: Ye
authors: Donn H
authors: DH
authors: Beighley
authors: Jiuhuan
authors: J
authors: Feng
authors: Xing-You
authors: XY
authors: Gu
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D016000:Cluster Analysis; D017343:Genes, Plant; D005838:Genotype; D018525:Germination / Q000235:genetics; D005875:Gibberellins / Q000096:biosynthesis / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D057445:Plant Dormancy / Q000235:genetics*; D040641:Quantitative Trait Loci; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
chemical_list: D005875:Gibberellins; D000516:alpha-Amylases
keywords: gibberellin signaling; plant height; quantitative trait locus; seed dormancy; weedy rice
references: Genes Genet Syst. 2005 Oct;80(5):351-6
references: 16394586
references: Genetics. 2011 Dec;189(4):1515-24
references: 21954164
references: Genetics. 2004 Mar;166(3):1503-16
references: 15082564
references: Nat Rev Genet. 2001 Oct;2(10):815-22
references: 11584298
references: Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):17042-7
references: 17065317
references: Nature. 1999 Jul 15;400(6741):256-61
references: 10421366
references: Mol Ecol. 2011 Sep;20(18):3743-56
references: 21854475
references: Plant J. 2003 Jul;35(1):104-15
references: 12834406
references: Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5792-7
references: 20220098
references: Nature. 2005 Aug 11;436(7052):793-800
references: 16100779
references: DNA Res. 2002 Dec 31;9(6):199-207
references: 12597276
references: DNA Res. 2002 Feb 28;9(1):11-7
references: 11939564
references: Plant Physiol. 2002 Apr;128(4):1264-70
references: 11950975
references: Evolution. 1973 Jun;27(2):311-325
references: 28564784
references: Cell. 1991 Sep 6;66(5):895-905
references: 1889090
references: Theor Appl Genet. 2011 Sep;123(5):705-14
references: 21637999
references: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):9043-8
references: 12077303