title: Antiproliferative potential of Artemisia capillaris polysaccharide against human nasopharyngeal carcinoma cells.
abstract: One water-soluble polysaccharide fraction (WACP) was purified from the Artemisia capillaris by DEAE-cellulose anion-exchange and Sephacryl S-400 gel-permeation chromatography. Based on gas chromatography (GC) analysis, WACP was an arabinogalactan (AG), consisting of arabinose and galactose in the ratio of 4:2. Its molecular weight was about 5.8×10(4) Da. The present study demonstrated the anticancer potential of WACP on human nasopharyngeal carcinoma CNE-2 cells and elucidated its possible mechanisms. MTT and flow cytometric analysis identified that WACP had a potent anti-proliferation activity on CNE-2 cells by inducing apoptosis, which was parallel with the morphological changes of CNE-2 cells under inverted microscope. In addition, WACP triggered the apoptosis via the mitochondrial apoptotic pathway, which included the loss of mitochondrial membrane potential, release of cytochrome c and activation of caspase-3/9. Taken together, these results suggest that WACP has anticancer potential in the treatment of human nasopharyngeal carcinoma.
authors: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors: Guofang
authors: G
authors: Feng
authors: Xiaoqing
authors: X
authors: Wang
authors: Changxuan
authors: C
authors: You
authors: Xiaohua
authors: X
authors: Cheng
authors: Zeli
authors: Z
authors: Han
authors: Liang
authors: L
authors: Zong
authors: Chengyong
authors: C
authors: Zhou
authors: Meikui
authors: M
authors: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D019306:Artemisia / Q000737:chemistry*; D002277:Carcinoma; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D014867:Water; D045304:Cytochromes c; D053148:Caspase 3; D053453:Caspase 9