title: Primary human muscle precursor cells obtained from young and old donors produce similar proliferative, differentiation and senescent profiles in culture.
abstract: The myogenic behaviour of primary human muscle precursor cells (MPCs) obtained from young (aged 20-25 years) and elderly people (aged 67-82 years) was studied in culture. Cells were compared in terms of proliferation, DNA damage, time course and extent of myogenic marker expression during differentiation, fusion, size of the formed myotubes, secretion of the myogenic regulatory cytokine TGF-β1 and sensitivity to TGF-β1 treatment. No differences were observed between cells obtained from the young and elderly people. The cell populations were expanded in culture until replicative senescence. Cultures that maintained their initial proportion of myogenic cells (desmin positive) with passaging (n = 5) were studied and compared with cells from the same individuals in the non-senescent state. The senescent cells exhibited a greater number of cells with DNA damage (γ-H2AX positive), showed impaired expression of markers of differentiation, fused less well, formed smaller myotubes and secreted more TGF-β. The data strongly suggest that MPCs from young and elderly people have similar myogenic behaviour.
authors: Centre of Human & Aerospace Physiological Sciences, King's College London, London, SE1 1UL, UK.
authors: Mansour
authors: M
authors: Alsharidah
authors: Norman R
authors: NR
authors: Lazarus
authors: Tomasz E
authors: TE
authors: George
authors: Chibeza C
authors: CC
authors: Agley
authors: Cristiana P
authors: CP
authors: Velloso
authors: Stephen D R
authors: SD
authors: Harridge
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D002459:Cell Fusion; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D004249:DNA Damage; D003893:Desmin / Q000378:metabolism; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D032342:Muscle Cells / Q000166:cytology; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000502:physiology*; D055948:Sarcopenia; D053773:Transforming Growth Factor beta1 / Q000378:metabolism / Q000494:pharmacology; D055815:Young Adult
chemical_list: D015415:Biomarkers; D003893:Desmin; D019394:Ki-67 Antigen; D053773:Transforming Growth Factor beta1