title: Assessment of DNA damage by micellar electrokinetic chromatography.
abstract: A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of uncanonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR templates used in several fields of investigation.
authors: Department of Medicine, surgery and Health, University of Trieste, Trieste, Italy. fattorin@univ.trieste.it
authors: Paolo
authors: P
authors: Fattorini
authors: Giorgio
authors: G
authors: Marrubini
authors: Pierangela
authors: P
authors: Grignani
authors: Solange
authors: S
authors: Sorçaburu-Cigliero
authors: Carlo
authors: C
authors: Previderé
mesh_terms: D002021:Buffers; D002138:Calibration; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods / Q000592:standards; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004249:DNA Damage; D019075:Electrophoresis, Capillary / Q000379:methods; D053803:Forensic Genetics; D006801:Humans; D006868:Hydrolysis; D057230:Limit of Detection; D016133:Polymerase Chain Reaction; D012015:Reference Standards; D014544:Uridine Triphosphate / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D002021:Buffers; C018009:5-bromouridine triphosphate; D004247:DNA; D014544:Uridine Triphosphate