title: Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening.
abstract: Although a great deal of progress has been made toward understanding the role of abscisic acid (ABA) in fruit ripening, many components in the ABA signalling pathway remain to be elucidated. Here, a strawberry gene homologous to the Arabidopsis gene ABI1, named FaABI1, was isolated and characterized. The 1641bp cDNA includes an intact open reading frame that encodes a deduced protein of 546 amino acids, in which putative conserved domains were determined by homology analysis. Transcriptional analysis showed that the levels of FaABI1 mRNA expression declined rapidly during strawberry fruit development as evidenced by real-time PCR, semi-quantitative reverse transcription-PCR, and northern blotting analyses, suggesting that the Ser/Thr protein phosphatase PP2C1 encoded by FaABI1 may be involved in fruit ripening as a negative regulator. The results of Tobacco rattle virus-induced gene silencing and PBI121 vector-mediated overexpression suggested that the down- and up-regulation of FaABI1 mRNA expression levels in degreening strawberry fruit could promote and inhibit ripening, respectively. Furthermore, alteration of FaABI1 expression could differentially regulate the transcripts of a set of both ABA-responsive and ripening-related genes, including ABI3, ABI4, ABI5, SnRK2, ABRE1, CHS, PG1, PL, CHI, F3H, DFR, ANS, and UFGT. Taken together, the data provide new evidence for an important role for ABA in regulating strawberry fruit ripening in the processes of which the type 2C protein phosphatase ABI1 serves as a negative regulator. Finally, a possible core mechanism underlying ABA perception and signalling transduction in strawberry fruit ripening is discussed.
authors: Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing 102206, PR China.
authors: Hai-Feng
authors: HF
authors: Jia
authors: Dong
authors: D
authors: Lu
authors: Jing-Hua
authors: JH
authors: Sun
authors: Chun-Li
authors: CL
authors: Li
authors: Yu
authors: Y
authors: Xing
authors: Ling
authors: L
authors: Qin
authors: Yuan-Yue
authors: YY
authors: Shen
mesh_terms: D000040:Abscisic Acid; D060054:Agrobacterium / Q000378:metabolism; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D031985:Fragaria / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D000071636:Protein Phosphatase 2C; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011994:Recombinant Proteins; D000040:Abscisic Acid; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C
references: Science. 1994 Jun 3;264(5164):1448-52
references: 7910981
references: Trends Plant Sci. 2007 Aug;12(8):343-51
references: 17629540
references: J Exp Bot. 2011 Nov;62(15):5659-69
references: 21873532
references: Plant Physiol. 2011 Sep;157(1):188-99
references: 21734113
references: Plant Physiol. 2012 Jan;158(1):283-98
references: 22108525
references: Plant Cell. 2002;14 Suppl:S15-45
references: 12045268
references: J Plant Physiol. 2010 Oct 15;167(15):1307-15
references: 20580122
references: Plant Physiol. 2003 Sep;133(1):135-44
references: 12970481
references: Nature. 1998 Feb 19;391(6669):806-11
references: 9486653
references: Plant Cell. 1997 May;9(5):759-71
references: 9165752
references: Plant Signal Behav. 2011 Dec;6(12):1950-3
references: 22095148
references: Science. 2009 May 22;324(5930):1064-8
references: 19407143
references: Plant J. 2002 Sep;31(6):777-86
references: 12220268
references: Plant J. 2001 Feb;25(3):295-303
references: 11208021
references: Plant Cell. 2010 Jun;22(6):1909-35
references: 20543028
references: Plant Physiol. 1997 Jul;114(3):751-7
references: 9232866
references: Nature. 2009 Dec 3;462(7273):660-4
references: 19924127
references: Biochem J. 1991 Feb 1;273 ( Pt 3):733-8
references: 1847622
references: Science. 1994 Jun 3;264(5164):1452-5
references: 8197457
references: Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1938-43
references: 9465121
references: PLoS One. 2011 Apr 29;6(4):e19455
references: 21559331
references: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:199-222
references: 15012233
references: Nature. 2009 Dec 3;462(7273):609-14
references: 19855379
references: Planta. 2010 Jun;232(1):219-34
references: 20407788
references: Eur J Biochem. 1992 Nov 1;209(3):923-31
references: 1358611
references: Nature. 2009 Dec 3;462(7273):602-8
references: 19898420
references: Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):975-80
references: 9448270
references: Curr Opin Plant Biol. 2003 Oct;6(5):470-9
references: 12972048
references: J Exp Bot. 2011 Oct;62(14):5079-89
references: 21778181
references: Science. 2009 Dec 4;326(5958):1373-9
references: 19933100
references: J Biol Chem. 1989 Dec 25;264(36):21435-8
references: 2557326
references: Clin Exp Pharmacol Physiol. 2008 Feb;35(2):107-12
references: 18197890
references: BMC Genomics. 2008 Nov 20;9:550
references: 19021904
references: Nature. 2009 Dec 3;462(7273):665-8
references: 19898494
references: Plant Cell. 1999 Oct;11(10):1897-910
references: 10521520
references: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:101-125
references: 15012284