title: Desalting DNA by drop dialysis increases library size upon transformation.
abstract: It is often desirable to obtain gene libraries with the greatest possible number of variants. We tested two different methods for desalting the products of library ligation reactions (silica-based microcolumns and drop dialysis), and examined their effects on final library size. For both intramolecular and intermolecular ligation, desalting by drop dialysis yielded approximately 3-5 times more transformants than microcolumn purification.
authors: Institute of Natural Sciences, Massey University, Auckland, New Zealand.
authors: Mayank
authors: M
authors: Saraswat
authors: Ralph S
authors: RS
authors: Grand
authors: Wayne M
authors: WM
authors: Patrick
mesh_terms: D000327:Adsorption; D003001:Cloning, Molecular / Q000379:methods*; D015169:Colony Count, Microbial; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors / Q000302:isolation & purification*; D017551:Microdialysis / Q000379:methods* / Q000592:standards; D012492:Salts / Q000737:chemistry*; D014169:Transformation, Bacterial
chemical_list: D012492:Salts; D004247:DNA