title: Construction of an amperometric bilirubin biosensor based on covalent immobilization of bilirubin oxidase onto zirconia coated silica nanoparticles/chitosan hybrid film.
abstract: A method is described for the construction of a highly sensitive electrochemical biosensor for the detection of bilirubin. The sensor is based on covalent immobilization of bilirubin oxidase (BOx) onto zirconia coated silica nanoparticles (SiO2@ZrONPs)/chitosan (CHIT) composite electrodeposited onto Au electrode. The enzyme electrode was characterized by scanning electron microscopy (SEM), Fourier transform infra-red spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor showed optimum response within 2s at pH 8.5 (0.1M Tris-HCl) and 35°C, when operated at 20 mV s(-1). The biosensor exhibited excellent sensitivity (detection limit as 0.1 nM), fast response time and wider linear range (from 0.02 to 250 μM). Analytical recovery of added bilirubin was 95.56-97.0%. Within batch and between batch coefficients of variation were 3.2% and 3.35% respectively. The enzyme electrode was used 150 times over a period of 120 days, when stored at 4°C. The biosensor measured bilirubin levels in sera of apparently healthy and persons suffering from jaundice, which correlated well with a standard colorimetric method (r=0.99).
authors: Department of Biochemistry, MD University, Rohtak, Haryana, India.
authors: Bhawna
authors: B
authors: Batra
authors: Suman
authors: S
authors: Lata
authors: Sunny
authors: J S
authors: JS
authors: Rana
authors: Chandra Shekhar
authors: CS
authors: Pundir
mesh_terms: D001663:Bilirubin / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D012822:Silicon Dioxide; D048271:Chitosan; D015040:Zirconium; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D001663:Bilirubin; C028541:zirconium oxide