title: New biotransformation process for production of the fragrant compound γ-dodecalactone from 10-hydroxystearate by permeabilized Waltomyces lipofer cells.
abstract: A new biotransformation process for the production of the flavor lactone was developed by using permeabilized Waltomyces lipofer, which was selected as an efficient γ-dodecalactone-producing yeast among 10 oleaginous yeast strains. The optimal reaction conditions for γ-dodecalactone production by permeabilized W. lipofer cells were pH 6.5, 35°C, 200 rpm, 0.7 M Tris, 60 g/liter of 10-hydroxystearic acid, and 30 g/liter of cells. Under these conditions, nonpermeabilized cells produced 12 g/liter of γ-dodecalactone after 30 h, with a conversion yield of 21% (wt/wt) and a productivity of 0.4 g/liter/h, whereas permeabilized cells obtained after sequential treatments with 50% ethanol and 0.5% Triton X-100 produced 46 g/liter of γ-dodecalactone after 30 h, with a conversion yield of 76% (wt/wt) and a productivity of 1.5 g/liter/h. These values were 3.7- and 3.8-fold higher than those obtained using nonpermeabilized cells. These are the highest reported concentration, conversion yield, and productivity for the production of the bioflavor lactone.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors: Jung-Ung
authors: JU
authors: An
authors: Young-Chul
authors: YC
authors: Joo
authors: Deok-Kun
authors: DK
authors: Oh
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D001711:Biotransformation; D007218:Industrial Microbiology / Q000379:methods; D010539:Permeability; D004718:Saccharomycetales / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*
chemical_list: D013229:Stearic Acids; C070376:10-hydroxystearic acid; D015107:4-Butyrolactone; C517611:gamma-dodecalactone
references: Appl Microbiol Biotechnol. 2011 Feb;89(3):535-47
references: 20981417
references: Nat Commun. 2012;3:1112
references: 23047670
references: Biotechnol Bioeng. 1999 Jul 5;64(1):54-60
references: 10397839
references: CRC Crit Rev Food Sci Nutr. 1976 Sep;8(1):1-56
references: 801348
references: Appl Microbiol Biotechnol. 2004 Jun;64(6):787-93
references: 14673547
references: Enzyme Microb Technol. 2000 Dec;27(10):806-811
references: 11118590
references: J Agric Food Chem. 2001 Jun;49(6):2948-53
references: 11409991
references: Endocrinology. 2008 Mar;149(3):942-9
references: 18202123
references: Biotechnol Adv. 2011 Jan-Feb;29(1):75-83
references: 20837129
references: Biotechnol Prog. 2004 Mar-Apr;20(2):449-56
references: 15058989
references: Appl Microbiol Biotechnol. 2003 Jun;61(5-6):393-404
references: 12764554
references: Chirality. 1997;9(7):667-71
references: 9366027
references: Appl Environ Microbiol. 2001 Dec;67(12):5700-4
references: 11722925
references: Biotechnol Lett. 2012 Apr;34(4):649-54
references: 22160330
references: Appl Microbiol Biotechnol. 2001 Nov;57(4):515-20
references: 11762598
references: Microbiol Res. 2012 Apr 20;167(4):246-52
references: 22115771
references: Biochimie. 2012 Mar;94(3):907-15
references: 22203098
references: Chem Commun (Camb). 2011 Mar 7;47(9):2502-10
references: 21243161
references: Cell. 1997 Feb 21;88(4):431-4
references: 9038333
references: J Agric Food Chem. 2004 Nov 17;52(23):7075-83
references: 15537321
references: Trends Biotechnol. 2008 Dec;26(12):668-73
references: 18976825
references: J Agric Food Chem. 2001 Dec;49(12):5880-2
references: 11743778
references: Appl Environ Microbiol. 1993 Jan;59(1):281-4
references: 16348853