title: Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands.
abstract: The exocytosis of salivary proteins is mainly regulated by cAMP, although soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which mediate cAMP-dependent exocytic membrane fusion, have remained unidentified. Here we examined the effect of isoproterenol (ISO) and cytochalasin D (CyD) on the level of SNARE complexes in rat parotid glands. When SNARE complexes were immunoprecipitated by anti-SNAP23, the coprecipitation of VAMP2 was significantly increased in response to ISO and/or CyD, although the coprecipitation of VAMP8 or syntaxin 4 was scarcely augmented. These results suggest that the SNAP23-VAMP2 interaction plays a key role in cAMP-mediated exocytosis from parotid glands.
authors: Department of Biochemistry, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido 061-0293, Japan. takuma@hoku-iryo-u.ac.jp
authors: Taishin
authors: T
authors: Takuma
authors: Akiko
authors: A
authors: Shitara
authors: Toshiya
authors: T
authors: Arakawa
authors: Miki
authors: M
authors: Okayama
authors: Itaru
authors: I
authors: Mizoguchi
authors: Yoshifumi
authors: Y
authors: Tajima
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D015638:Cytochalasin D / Q000494:pharmacology; D004229:Dithiothreitol; D005033:Ethylmaleimide; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D047468:Immunoprecipitation; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010306:Parotid Gland / Q000187:drug effects* / Q000502:physiology; D011485:Protein Binding; D050765:Qa-SNARE Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D046509:Tissue Culture Techniques; D050988:Vesicle-Associated Membrane Protein 2 / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000318:Adrenergic beta-Agonists; D019384:Nucleic Acid Synthesis Inhibitors; D050765:Qa-SNARE Proteins; C498858:Snap23 protein, rat; C494706:Vamp2 protein, rat; D050988:Vesicle-Associated Membrane Protein 2; D033921:Vesicular Transport Proteins; D015638:Cytochalasin D; D000242:Cyclic AMP; D007545:Isoproterenol; D005033:Ethylmaleimide; D004229:Dithiothreitol