title: The impact of compact layer in biphasic scaffold on osteochondral tissue engineering.
abstract: The structure of an osteochondral biphasic scaffold is required to mimic native tissue, which owns a calcified layer associated with mechanical and separation function. The two phases of biphasic scaffold should possess efficient integration to provide chondrocytes and osteocytes with an independent living environment. In this study, a novel biphasic scaffold composed of a bony phase, chondral phase and compact layer was developed. The compact layer-free biphasic scaffold taken as control group was also fabricated. The purpose of current study was to evaluate the impact of the compact layer in the biphasic scaffold. Bony and chondral phases were seeded with autogeneic osteoblast- or chondrocyte-induced bone marrow stromal cells (BMSCs), respectively. The biphasic scaffolds-cells constructs were then implanted into osteochondral defects of rabbits' knees, and the regenerated osteochondral tissue was evaluated at 3 and 6 months after surgery. Anti-tensile and anti-shear properties of the compact layer-containing biphasic scaffold were significantly higher than those of the compact layer-free biphasic scaffold in vitro. Furthermore, in vivo studies revealed superior macroscopic scores, glycosaminoglycan (GAG) and collagen content, micro tomograph imaging results, and histological properties of regenerated tissue in the compact layer-containing biphasic scaffold compared to the control group. These results indicated that the compact layer could significantly enhance the biomechanical properties of biphasic scaffold in vitro and regeneration of osteochondral tissue in vivo, and thus represented a promising approach to osteochondral tissue engineering.
authors: Institute of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors: Hu
authors: H
authors: Da
authors: Shuai-Jun
authors: SJ
authors: Jia
authors: Guo-Lin
authors: GL
authors: Meng
authors: Jian-Hua
authors: JH
authors: Cheng
authors: Wei
authors: W
authors: Zhou
authors: Zhuo
authors: Z
authors: Xiong
authors: Yun-Jing
authors: YJ
authors: Mu
authors: Jian
authors: J
authors: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002358:Cartilage, Articular / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000473:pathology; D002469:Cell Separation; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D011817:Rabbits; D011859:Radiography; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials
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