title: Vitamin C Up-regulates Expression of CD80, CD86 and MHC Class II on Dendritic Cell Line, DC-1 Via the Activation of p38 MAPK.
abstract: Vitamin C is an essential water-soluble nutrient which primarily exerts its effect on host defense mechanisms and immune homeostasis, but the mechanism related to immune-potentiation is poorly understood. Since dendritic cells (DCs) are known as a potent antigen presenting cell (APC) that could enhance the antigen specific immune responses, we investigate the effects of vitamin C on activation of DCs and its related mechanism by using dendritic cell lines, DC-1. First, we found that there was no damage on DC-1 by 2.5 mM of vitamin C. In the presence of vitamin C, the expression of CD80, CD86, and MHC molecules was increased, but it was decreased by the pre-treatment of SB203580, p38 MAPK-specific inhibitor. We confirmed the phosphorylation of p38 MAPK was increased by the treatment of vitamin C. Taken together, these results suggest that vitamin C could enhance the activity of dendritic cells via the up-regulation of the expression of CD80, CD86, and MHC molecules and the activation of p38 MAPK is related to this process.
authors: School of Korean Medicine, Pusan National University, Pusan 626-870, Korea.
authors: Hyung Woo
authors: HW
authors: Kim
authors: Su In
authors: SI
authors: Cho
authors: Seyeon
authors: S
authors: Bae
authors: Hyemin
authors: H
authors: Kim
authors: Yejin
authors: Y
authors: Kim
authors: Young-Il
authors: YI
authors: Hwang
authors: Jae Seung
authors: JS
authors: Kang
authors: Wang Jae
authors: WJ
authors: Lee
keywords: CD80; CD86; Dendritic cell; MHC class II; Vitamin C; p38 MAPK
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