title: Visualizing Hg2+ ions in living cells using a FRET-based fluorescent sensor.
abstract: A novel FRET fluorescent sensor for Hg2+ imaging in living cells is rationally designed based on a coumarin-rhodamine platform. RBC1 exhibit high selectivity and excellent sensitivity in both absorbance and fluorescence detection of Hg2+ in aqueous solution. After addition of increasing concentrations of Hg2+, it result in the decrease of coumarin emission at 467 nm and a new emission profile of rhodamine at 590 nm gradually increased. The response time to Hg2+ is less than 2 min, and other metal ions including Fe2+, Mn2+, Ni2+, Co2+, Cu2+, Zn2+, Cd2+, Pb2+, and Cr3+ had no interference. In addition, fluorescent imaging of Hg2+ in A375 cells is also successfully demonstrated. The design strategy of two fluorophores switching in this work would help to extend the development of FRET fluorescent sensors.
authors: State Key Laboratory of Materials-Oriented Chemical Engineering and College of Science, Nanjing University of Technology, Nanjing 210009, PR China.
authors: Yi
authors: Y
authors: Zhou
authors: Kaihui
authors: K
authors: Chu
authors: Haifu
authors: H
authors: Zhen
authors: Yuan
authors: Y
authors: Fang
authors: Cheng
authors: C
authors: Yao
mesh_terms: D002413:Cations, Divalent / Q000032:analysis; D045744:Cell Line, Tumor; D002470:Cell Survival; D003374:Coumarins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D002413:Cations, Divalent; D003374:Coumarins; D005456:Fluorescent Dyes; D012235:Rhodamines; D008628:Mercury