title: Fluorescence imaging in the last two decades.
abstract: In commemoration of the 20th anniversary of the molecular cloning of the gene for the green fluorescent protein from the jellyfish Aequorea victoria, I would like to reflect on the development of new fluorescence imaging technology in the last two decades. As this technology has become increasingly diversified, it has become more and more of a challenge to come up with a comprehensive and exhaustive review of it. Here I will focus on optogenetics and large-scale, three-dimensional reconstruction. Those two technological innovations have been achieved in the neuroscience community owing to the combined efforts of molecular biologists and light microscopists. In addition, modern fluorescence imaging has indeed improved our understanding of the spatiotemporal regulation of fundamental biological functions at cellular level. As an example, I will introduce some findings we made regarding the movement of biomolecules across the nuclear membrane. The above-mentioned imaging approaches are possible today but were impossible two decades ago.
authors: Brain Science Institute, RIKEN and Life Function and Dynamics, ERATO, JST, 2-1 Hirosawa, Wako-city, Saitama, Japan. matsushi@brain.riken.jp
authors: Atsushi
authors: A
authors: Miyawaki
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional; D009685:Nuclear Envelope / Q000235:genetics / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods*; D037401:Scyphozoa / Q000235:genetics / Q000648:ultrastructure
chemical_list: D049452:Green Fluorescent Proteins
references: Nat Biotechnol. 2003 Nov;21(11):1369-77
references: 14595365
references: Nature. 2007 Nov 1;450(7166):56-62
references: 17972876
references: Neuron. 2009 Apr 16;62(1):17-29
references: 19376064
references: Curr Opin Neurobiol. 2012 Feb;22(1):18-23
references: 22104761
references: Science. 1994 Feb 11;263(5148):802-5
references: 8303295
references: Neuron. 2006 Jan 5;49(1):81-94
references: 16387641
references: Science. 2011 Jul 15;333(6040):345-8
references: 21764748
references: Neuron. 2000 Oct;28(1):41-51
references: 11086982
references: Gene. 1992 Feb 15;111(2):229-33
references: 1347277
references: Nat Rev Mol Cell Biol. 2008 Dec;9(12):929-43
references: 19002208
references: J Cell Comp Physiol. 1962 Jun;59:223-39
references: 13911999
references: Neuron. 2007 Jul 5;55(1):25-36
references: 17610815
references: Curr Opin Biotechnol. 2005 Feb;16(1):35-40
references: 15722013
references: Nat Neurosci. 2011 Aug 30;14(11):1481-8
references: 21878933
references: J Anat. 2003 Feb;202(2):175-81
references: 12647867
references: Science. 2004 Nov 19;306(5700):1370-3
references: 15550670
references: Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18252-7
references: 16344470
references: Trends Cell Biol. 2009 Nov;19(11):555-65
references: 19836954
references: Methods Cell Biol. 2012;111:157-73
references: 22857928
references: Nature. 1998 Jul 9;394(6689):192-5
references: 9671304
references: Neuron. 2011 Jul 14;71(1):9-34
references: 21745635
references: Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10914-9
references: 20534555
references: Nat Methods. 2005 Dec;2(12):932-40
references: 16299478
references: Nat Methods. 2012 Dec;9(12):1198-201
references: 23085613
references: Nat Methods. 2011 Nov 27;9(1):90-5
references: 22120467
references: PLoS One. 2012;7(3):e33916
references: 22479475
references: Nat Methods. 2007 Apr;4(4):331-6
references: 17384643
references: Nature. 2009 May 28;459(7246):523-7
references: 19478777
references: Curr Opin Chem Biol. 2010 Feb;14(1):10-4
references: 19897404
references: PLoS Biol. 2011 Apr;9(4):e1001041
references: 21483721
references: Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4411-6
references: 18332427
references: Nat Biotechnol. 2003 Jan;21(1):86-9
references: 12469133
references: Physiol Rev. 2010 Jul;90(3):1103-63
references: 20664080
references: Chem Soc Rev. 2009 Oct;38(10):2887-921
references: 19771335
references: Science. 2002 Sep 13;297(5588):1873-7
references: 12228718
references: Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8740-5
references: 15939876
references: Biophys J. 2009 Oct 7;97(7):1891-7
references: 19804719
references: Biophys J. 2009 Sep 2;97(5):1288-94
references: 19720016
references: Science. 2002 Apr 19;296(5567):541-5
references: 11964482
references: Organogenesis. 2009 Oct;5(4):227-30
references: 20539742
references: EMBO Rep. 2005 Mar;6(3):233-8
references: 15731765
references: Nat Neurosci. 2012 May 27;15(7):1047-53
references: 22634730
references: Nat Rev Mol Cell Biol. 2005 Nov;6(11):885-91
references: 16167053
references: Annu Rev Biochem. 1998;67:509-44
references: 9759496
references: F1000 Biol Rep. 2011;3:11
references: 21876722
references: Annu Rev Biochem. 2009;78:993-1016
references: 19489737
references: Cell. 2007 Mar 23;128(6):1205-17
references: 17382887
references: PLoS Biol. 2004 Nov;2(11):e329
references: 15514700
references: Annu Rev Neurosci. 2011;34:389-412
references: 21692661
references: Curr Opin Struct Biol. 2012 Oct;22(5):679-88
references: 23000031
references: Science. 1998 Jul 10;281(5374):269-72
references: 9657724
references: Neuron. 2012 Mar 8;73(5):862-85
references: 22405199
references: Nature. 2007 Apr 5;446(7136):633-9
references: 17410168
references: Nat Methods. 2010 Aug;7(8):643-9
references: 20622860
references: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12651-6
references: 12271129
references: Curr Opin Neurobiol. 2008 Dec;18(6):633-41
references: 19361979
references: Curr Opin Neurobiol. 2008 Jun;18(3):346-53
references: 18801435
references: J Cell Biol. 2010 Jul 26;190(2):165-75
references: 20643879
references: Nat Biotechnol. 1999 Oct;17(10):969-73
references: 10504696