title: Responses of biofilm characteristics to variations in temperature and NH4(+)-N loading in a moving-bed biofilm reactor treating micro-polluted raw water.
abstract: A pilot-scale moving-bed biofilm reactor (MBBR) for biological treatment of micro-polluted raw water was operated over 400days to investigate the responses of biofilm characteristics and nitrification performance to variations in temperature and NH4(+)-N loading. The mean removal efficiency of NH4(+)-N in the MBBR reached 71.4±26.9%, and batch experiments were performed to study nitrification kinetics for better process understanding. Seven physical-chemical parameters, including volatile solids (VS), polysaccharides (PS) and phospholipids (PL) increased firstly, and then rapidly decreased with increasing temperature and NH4(+)-N loading, and properly characterized the attached biomass during biofilm development and detachment in the MBBR. The biofilm compositions were described by six ratios, e.g., PS/VS and PL/VS ratios showed different variation trends, indicating different responses of PS and PL to the changes in temperature and NH4(+)-N loading. Furthermore, fluorescent in situ hybridization (FISH) analysis revealed that increased NH4(+)-N loadings caused an enrichment of the nitrifying biofilm.
authors: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
authors: Shuangfu
authors: S
authors: Zhang
authors: Yayi
authors: Y
authors: Wang
authors: Weitao
authors: W
authors: He
authors: Min
authors: M
authors: Wu
authors: Meiyan
authors: M
authors: Xing
authors: Jian
authors: J
authors: Yang
authors: Naiyun
authors: N
authors: Gao
authors: Daqiang
authors: D
authors: Yin
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D000644:Quaternary Ammonium Compounds; D014874:Water Pollutants, Chemical