title: [Effects of BDNF antibody to block IL-1beta expression and lung injury in rats subjected to brain ischemia].
abstract: OBJECTIVE
  To explore the effects of brain-derived neurotrophic factor (BDNF) antibody to block lung injury and interleukin-1beta (IL-1beta) expression in the rats with brain ischemia.
  
  
  METHODS
  Thirty nine SD rats were divided into sham group, brain ischemia lung injury (BILI) group, and BDNF antibody treated group. Inflammatory lung injury was induced by brain ischemia in the later two groups, and BDNF antibody was given through intraperitoneal injection to the rats for 3 days in BDNF antibody group. Lung tissue samples were harvested from the rats in each group at the 3rd day after brain ischemia. Lung edema degree was evaluated by HE staining. The protein expressions of IL-1beta in the lung tissues were measured by the methods of immunohistochemistry (n=5) and Western blot (n=8).
  
  
  RESULTS
  The increased immunostaining of IL-1beta were found in the lung tissue at 3 days after brain ischemia, which indicated the upregulated expression of IL-1beta protein in the lung injury induced by cerebral ischemia. The block of BDNF antibody resulted in a significant decrease of IL-1beta expression, as well as the decrease of lung edema.
  
  
  CONCLUSIONS
  BDNF, as a crucial factor, could regulate airway inflammation injury in brain ischemia rats via activating IL-1beta expression.
authors: Cadre's Ward, Anhui Provincial Hospital, Hefei 230001, China.
authors: Rong
authors: R
authors: Rong
authors: Ting-Ting
authors: TT
authors: Yang
authors: Jin-Tao
authors: JT
authors: Li
authors: Qi-Qin
authors: QQ
authors: Dan
authors: Yun-Hui
authors: YH
authors: Zhang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D055370:Lung Injury / Q000209:etiology / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000906:Antibodies; D019208:Brain-Derived Neurotrophic Factor; D053583:Interleukin-1beta; D012333:RNA, Messenger