title: Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues.
abstract: This study investigated the subcellular location of mung bean (Vigna radiata) 8S globulin in transient expression systems as well as in tobacco (Nicotiana tabacum) BY-2 cells and different tissues from a transgenic Arabidopsis (Arabidopsis thaliana) line stably expressing this storage globulin. When transiently expressed in protoplasts from both BY-2 cells and Arabidopsis suspension cultured cells, the 8S globulin located to structures that were neither Golgi nor pre-vacuolar compartments (PVCs). Immunogold electron microscopy of the transgenics reveals the 8S globulin-positive structures to be small, spherical, ribosome-covered endoplasmic reticulum (ER)-derived bodies. In BY-2 cells and all vegetative cells, the 8S globulin was present as a pro-form. However, in Arabidopsis embryos, with the onset of endogenous storage protein synthesis, the 8S globulin exited the ER and passed through the PVC to the protein storage vacuole where it was processed to its smaller mature form. These results clearly demonstrated that, when taken out of context and expressed in vegetative cells, the mung bean 8S storage globulin cannot exit the ER, and indicate that natural targeting of storage proteins to the vacuole should be better studied in the maturing seed.
authors: School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, PR China.
authors: Junqi
authors: J
authors: Wang
authors: Jinbo
authors: J
authors: Shen
authors: Yi
authors: Y
authors: Cai
authors: David G
authors: DG
authors: Robinson
authors: Liwen
authors: L
authors: Jiang
mesh_terms: D055516:2S Albumins, Plant / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism; D007887:Fabaceae / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006056:Golgi Apparatus / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D021381:Protein Transport; D011523:Protoplasts; D012639:Seeds / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D055516:2S Albumins, Plant
references: Plant Cell Physiol. 2005 Jan;46(1):245-9
references: 15659439
references: Plant J. 2008 Dec;56(5):824-39
references: 18680561
references: Plant J. 2011 Mar;65(6):882-96
references: 21251105
references: Plant Cell. 2006 Oct;18(10):2567-81
references: 17012602
references: J Exp Bot. 2009;60(2):629-39
references: 19129164
references: Biosci Biotechnol Biochem. 2010;74(12):2529-31
references: 21150096
references: J Exp Bot. 2009;60(11):3075-83
references: 19436047
references: Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):16095-100
references: 14657332
references: J Exp Bot. 2011 Aug;62(13):4507-20
references: 21617248
references: J Agric Food Chem. 2004 May 5;52(9):2552-60
references: 15113156
references: J Agric Food Chem. 2001 Mar;49(3):1552-8
references: 11312895
references: J Cell Biol. 1995 Sep;130(6):1307-18
references: 7559754
references: Science. 1993 Nov 12;262(5136):1054-6
references: 8235623
references: Traffic. 2007 Oct;8(10):1452-64
references: 17696967
references: Plant Physiol. 2002 Nov;130(3):1102-8
references: 12427977
references: J Cell Biol. 2000 Aug 21;150(4):755-70
references: 10953001
references: Plant Cell Physiol. 2005 Jul;46(7):1140-8
references: 15919674
references: Plant Physiol. 2006 Nov;142(3):945-62
references: 16980567
references: FEBS J. 2011 Jan;278(1):59-68
references: 21078125
references: Plant J. 1998 Dec;16(6):735-43
references: 10069079
references: Biosci Biotechnol Biochem. 2009 Jan;73(1):53-60
references: 19129658
references: Plant Physiol. 2007 Apr;143(4):1628-39
references: 17322331
references: Plant Cell. 2007 Jan;19(1):296-319
references: 17209124
references: Plant Cell. 2005 Nov;17(11):3066-80
references: 16227454
references: Traffic. 2008 Sep;9(10):1564-70
references: 18537999
references: Plant J. 2003 Feb;33(3):493-502
references: 12581307
references: Protoplasma. 2012 Apr;249(2):379-92
references: 21614590
references: J Exp Bot. 2012 Feb;63(3):1367-80
references: 22143915
references: Plant Cell. 1998 May;10(5):825-36
references: 9596640
references: Nat Protoc. 2007;2(10):2348-53
references: 17947977
references: Plant J. 2009 Dec;60(5):865-81
references: 19709389
references: J Cell Sci. 1995 Jan;108 ( Pt 1):299-310
references: 7738107
references: Plant Cell. 2003 May;15(5):1057-70
references: 12724533
references: Plant Cell. 1998 Jun;10(6):1031-42
references: 9634590
references: Plant Cell Physiol. 2002 Jul;43(7):726-42
references: 12154135
references: J Agric Food Chem. 2006 Aug 9;54(16):6005-10
references: 16881708
references: J Cell Biol. 1998 Nov 30;143(5):1183-99
references: 9832548
references: J Cell Sci. 1996 Oct;109 ( Pt 10):2539-50
references: 8923215
references: Plant J. 2010 Nov;64(4):577-88
references: 20807215
references: Mol Plant. 2011 Sep;4(5):854-68
references: 21493745
references: Plant Cell. 2012 May;24(5):2086-104
references: 22570441
references: Plant Cell. 2006 Oct;18(10):2608-21
references: 17041149
references: Plant Physiol. 2008 Jul;147(3):954-61
references: 18612072
references: Plant Cell. 2004 Mar;16(3):672-93
references: 14973159
references: Curr Opin Plant Biol. 2008 Dec;11(6):672-9
references: 18824401
references: J Exp Bot. 2011 May;62(8):2949-57
references: 21357769
references: Plant Mol Biol. 1998 Sep;38(1-2):77-99
references: 9738961
references: Traffic. 2012 Jul;13(7):1023-40
references: 22486829
references: J Exp Bot. 2008;59(10):2815-29
references: 18540021
references: J Mol Evol. 1995 Dec;41(6):1057-69
references: 8587104
references: Plant Cell. 1999 Apr;11(4):601-14
references: 10213781
references: Trends Plant Sci. 2007 Nov;12(11):497-505
references: 17920331
references: Trends Biotechnol. 2002 Mar;20(3):99-102
references: 11841859
references: Plant Cell. 1994 Dec;6(12):1911-1922
references: 12244234
references: Plant Cell. 2010 Dec;22(12):4009-30
references: 21193573
references: Trends Plant Sci. 2012 Jul;17(7):431-9
references: 22502774
references: Plant Cell. 1995 Jul;7(7):945-56
references: 7640527
references: J Cell Biol. 2001 Jan 8;152(1):41-50
references: 11149919
references: J Cell Biol. 2001 Dec 10;155(6):991-1002
references: 11739409
references: Trends Cell Biol. 2002 Aug;12(8):362-7
references: 12191912
references: Plant Physiol. 2007 Apr;143(4):1601-14
references: 17307897
references: Trends Plant Sci. 2002 Aug;7(8):340-4
references: 12167328
references: Plant Cell. 2001 May;13(5):1165-78
references: 11340189
references: Plant Physiol. 2004 Nov;136(3):3447-56
references: 15502013
references: Plant Cell. 1997 Oct;9(10):1869-80
references: 9368420
references: Biol Chem Hoppe Seyler. 1993 Sep;374(9):887-94
references: 8267881
references: Plant Physiol. 2004 Nov;136(3):3414-9
references: 15542494
references: Plant Physiol. 2008 Aug;147(4):1637-45
references: 18508957
references: Plant Cell. 2005 May;17(5):1513-31
references: 15805489
references: Annu Rev Food Sci Technol. 2011;2:59-73
references: 22129375
references: Plant Cell. 2001 Sep;13(9):2005-20
references: 11549760
references: Trends Plant Sci. 2012 Oct;17(10):606-15
references: 22784825
references: Planta. 2010 May;231(6):1261-76
references: 20217123
references: J Exp Bot. 2011 Oct;62(14):5063-78
references: 21725030
references: Planta. 1983 Apr;157(5):401-10
references: 24264336