title: [Dynamic detection of regulatory T cells in murine mammary carcinoma model and its significance].
abstract: OBJECTIVE
  To analyze the dynamic change of regulatory T cells in experimental murine mammary carcinoma model, and to investigate their effect on tumor progress.
  
  
  METHODS
  Mouse mammary carcinoma models were established. The percentages of CD4+ CD25+ and CD4+ FOXP3+ regulatory T cells in the CD4+ T cells in tumor tissue, tumor draining lymph node and spleen were measured by FACS at 3 different time points after tumor challenge. The expression of CD25+ FOXP3 in CD4+ T cells was analyzed, and the expression of CD4+ T cells in T cells (CD3+) of tumor tissue.
  
  
  RESULTS
  Compared with the normal control group, In mouse mammary carcinoma models, The percentages of CD4+ T cells to lymphocytes in lymph node and spleen were decreased in the late stage (P<0.05). The percentages of CD4+ CD25+ and CD4+ FOXP3+ regulatory T cells in the CD4+ T cells were markedly increased (P<0.05), The percentages of CD25+ FOXP3+ regulatory T cells in the CD4+ T cells were markedly increased also (P<0.05). In the tumor tissue, the percentages of CD4+ T cells and CD4+ CD25+ regulatory T cells in T cells (CD3+) were increased in three weeks after the tumor challenge than one week (P< 0. 05). The percentages of CD4+ CD25+ and CD4+ FOXP3+ regulatory T cells in the CD4+ T cells have no change between one week and three weeks after tumor planting (P>0.05).
  
  
  CONCLUSIONS
  Regulatory T cells were significantly increased in malignant tumor model, and closely related to the development of tumor.
authors: Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors: Hong-Mei
authors: HM
authors: Zhu
authors: Ting
authors: T
authors: Hu
authors: Mei
authors: M
authors: Yang
authors: He
authors: H
authors: Wang
authors: Xin-Lian
authors: XL
authors: Chen
authors: Xiao-Yan
authors: XY
authors: Xie
authors: Shan-Ling
authors: SL
authors: Liu
mesh_terms: D000818:Animals; D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse