title: Quantification of arginine and dimethylated arginines in human plasma by field-amplified sample injection capillary electrophoresis UV detection.
abstract: We report a capillary electrophoresis method by field-amplified sample injection (FASI) for the separation and detection of free plasma arginine and dimethylated arginines. Protein elimination from plasma was performed by acetonitrile/ammonia precipitation. Supernatants were dried, reswollen in water, and directly injected on capillary without complex cleanup by solid-phase extraction and/or tedious sample derivatization procedures. Analytes were baseline-separated within 22 min by using 50 mmol/L Tris phosphate pH 2.3 as running buffer. Due to the stacking effects of electrokinetic injection, it is possible to perform a considerable on-line preconcentration of the analytes before running the electrophoresis. This procedure allows to reach a detection limit in real sample of 10 nmol/L for dimethylated arginines and 20 nmol/L for arginine. Recovery of plasma ADMA was 99-104 % and inter-day CV was less than 3 %.
authors: Department Biomedical Sciences, University of Sassari, Sassari, Italy. azinellu@uniss.it
authors: Angelo
authors: A
authors: Zinellu
authors: Salvatore
authors: S
authors: Sotgia
authors: Luca
authors: L
authors: Deiana
authors: Ciriaco
authors: C
authors: Carru
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D002021:Buffers; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D014466:Ultraviolet Rays
chemical_list: D002021:Buffers; C487735:dimethylarginine; C018524:N,N-dimethylarginine; D001120:Arginine