title: CD4(+) T-cells are unable to express the HIV natural resistance factor globotriosylceramide.
abstract: Globotriaosylceramide (Gb(3)) is a cell surface-expressed natural resistance factor for HIV infection, but, its expression in human T-cells remains unknown. Therefore, Gb(3) in resting or activated CD4(+) T-cells was assessed by flow cytometry and thin layer chromatography of cell extracts. We found the majority of CD4(+) T-cells, whether resting or activated, do not express Gb(3) at significant levels (<2% positive cells). Thus, HIV treatment or prevention strategies must focus on development of soluble Gb(3) analogues for inhibition of HIV infection.
authors: aDepartment of Medicine, University of Toronto bMolecular Structure and Function, Hospital for Sick Children Research Institute cCanadian Blood Services, Research and Development dDepartments of Laboratory Medicine and Pathobiology, and Biochemistry, University of Toronto, Toronto, Ontario M5G 2M1, Canada.
authors: Minji
authors: M
authors: Kim
authors: Beth
authors: B
authors: Binnington
authors: Darinka
authors: D
authors: Sakac
authors: Clifford A
authors: CA
authors: Lingwood
authors: Donald R
authors: DR
authors: Branch
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D014281:Trihexosylceramides / Q000032:analysis / Q000172:deficiency*
chemical_list: D014281:Trihexosylceramides; C018549:globotriaosylceramide