title: [Isolation and characterization of an Enterococcus strain from Tibetan alpine meadow soil].
abstract: OBJECTIVE
  We studied the physiological, biochemical properties and metabolism of Enterococcus strain CJ-1 from high-altitude soil in Namtso, Tibet.
  
  
  METHODS
  Strain CJ-1(T) was isolated from the soil of Namtso alpine meadow soil by Hungate anaerobic technique. Through physiological, biochemical and phylogenetic analysis, we identified the strain CJ-1.
  
  
  RESULTS
  Strain CJ-1 was Gram-positive and facultative anaerobe, 1 -1.5 microm in diameter. CJ-1(T) was atrichia nonmotile cocci, and always occurred in pairs. CJ-1(T) occurred in the presence of 0% -7% NaCl (optimum at 5%), pH 5.0 - 8.5 (optimum 7.0) and temperature between 10 degrees C and 50 degrees C (optimum at 25 degrees C). CJ-1 could metabolize many carbon sources including cellobiose, melezitose and ribose. Metabolites of cellobiose were lactic acid, acetic acid, propionic acid, butyrate, CO2, and little H2. The mol% G + C content of the genomic DNA was 39.2 mol%. 16S rRNA gene sequence analysis of the above strain showed the highest similarity of 95.9% with its closest phylogenetic neighbor Enterococcus aquimarinus. The strain can degrade cellobiose which act as intermediate metabolites in the methane fermentation process.
  
  
  CONCLUSIONS
  CJ-1(T) can degrade cellobiose. CJ-1(T) represents a novel species in the genus fermentation process. Enterococcus.
authors: Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China. yliso1@yahoo.com.cn
authors: Lei
authors: L
authors: Yang
authors: Yu
authors: Y
authors: Deng
authors: Hui
authors: H
authors: Zhang
authors: Qingyun
authors: Q
authors: Diao
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016983:Enterococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D018609:Tibet
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S