title: Surface plasmon resonance for measuring interactions of proteins with lipid membranes.
abstract: Surface plasmon resonance (SPR) is an established method for studying molecular interactions in real time. It allows obtaining qualitative and quantitative data on interactions of proteins with lipid membranes. In most of the approaches, a lipid membrane or a membrane-mimetic surface is prepared on the surface of Biacore (GE Healthcare) sensor chips HPA or L1, and the studied protein is then injected across the surface. Here, we provide an overview of SPR in protein-membrane interactions, different approaches described in the literature, and a general protocol for conducting an SPR experiment including lipid membranes, together with some experimental considerations.
authors: Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors: Vesna
authors: V
authors: Hodnik
authors: Gregor
authors: G
authors: Anderluh
mesh_terms: D000818:Animals; D008051:Lipid Bilayers / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D054467:Phospholipases A2 / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D011506:Proteins; D054467:Phospholipases A2