title: Characterization of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression.
abstract: Red pigment concentrating hormone (RPCH) is a member of the chromatophorotropic hormones and, in crustaceans, it is synthesized in the eyestalk. We have isolated a full-length cDNA for a RPCH preprohormone gene (Scyol-RPCH) from the eyestalks of female mud crabs, Scylla olivacea. The open reading frame consists of 642 nucleotides, and encodes a deduced 108 amino acid precursor protein, which includes a signal peptide, the RPCH (pQLNFSPGWamide), and an associated peptide. We show that the mud crab RPCH peptide exhibits 100% identity with 15 other decapods. Expression of Scyol-RPCH within adult mud crab takes place in the eyestalk, brain, and ventral nerve cord, comprising subesophageal ganglion, thoracic ganglion, and abdominal ganglion. In situ hybridization demonstrates specific expression within neuronal clusters 1, 2, 3, and 4 of the eyestalk X-organ, clusters 6, 8, 9, 10, and 17 of the brain, and in neuronal clusters of the ventral nerve cord. We found that administration of 5-HT up-regulates RPCH gene expression in the eyestalk, suggesting that RPCH may play a role as a downstream hormone of 5-HT.
authors: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand. moo_natt@hotmail.com
authors: Napamanee
authors: N
authors: Kornthong
authors: Charoonroj
authors: C
authors: Chotwiwatthanakun
authors: Piyachat
authors: P
authors: Chansela
authors: Yotsawan
authors: Y
authors: Tinikul
authors: Scott F
authors: SF
authors: Cummins
authors: Peter J
authors: PJ
authors: Hanna
authors: Prasert
authors: P
authors: Sobhon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007447:Invertebrate Hormones / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000096:biosynthesis* / Q000235:genetics; D010802:Phylogeny; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives*; D016415:Sequence Alignment; D012701:Serotonin / Q000494:pharmacology*; D014018:Tissue Distribution
chemical_list: D007447:Invertebrate Hormones; D009842:Oligopeptides; D011498:Protein Precursors; D012701:Serotonin; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid