title: Acetylated Histone H3K9 is associated with meiotic recombination hotspots, and plays a role in recombination redundantly with other factors including the H3K4 methylase Set1 in fission yeast.
abstract: Histone modifications are associated with meiotic recombination hotspots, discrete sites with augmented recombination frequency. For example, trimethylation of histone H3 lysine4 (H3K4me3) marks most hotspots in budding yeast and mouse. Modified histones are known to regulate meiotic recombination partly by promoting DNA double-strand break (DSB) formation at hotspots, but the role and precise landscape of involved modifications remain unclear. Here, we studied hotspot-associated modifications in fission yeast and found general features: acetylation of H3 lysine9 (H3K9ac) is elevated, and H3K4me3 is not significantly enriched. Mutating H3K9 to non-acetylatable alanine mildly reduced levels of the DSB-inducing protein Rec12 (the fission yeast homologue of Spo11) and DSB at hotspots, indicating that H3K9ac may be involved in DSB formation by enhancing the interaction between Rec12 and hotspots. In addition, we found that the lack of the H3K4 methyltransferase Set1 generally increased Rec12 binding to chromatin but partially reduced DSB formation at some loci, suggesting that Set1 is also involved in DSB formation. These results suggest that meiotic DSB formation is redundantly regulated by multiple chromatin-related factors including H3K9ac and Set1 in fission yeast.
authors: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan.
authors: Shintaro
authors: S
authors: Yamada
authors: Kunihiro
authors: K
authors: Ohta
authors: Takatomi
authors: T
authors: Yamada
mesh_terms: D000107:Acetylation; D002843:Chromatin / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D017353:Gene Deletion; D016681:Genome, Fungal; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000502:physiology*; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008540:Meiosis / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D002843:Chromatin; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C456270:rec12 protein, S pombe; D000099257:Meiotic Recombination Protein SPO11; C472830:Set1 protein, S pombe
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