title: Otoprotective effects of erythropoietin on Cdh23erl/erl mice.
abstract: The Cdh23(erl/erl) mice are a novel mouse model for DFNB12 and are characterized by progressive hearing loss. In this study, erythropoietin (EPO) was given to the Cdh23(erl/erl) mice by intraperitoneal injection every other day from P7 for 7 weeks. Phosphate-buffered saline-treated or untreated Cdh23(erl/erl) mice were used as controls. Auditory-evoked brainstem response (ABR) thresholds and distortion product oto-acoustic emission (DPOAE) were measured in the mouse groups at the age of 4, 6 and 8 weeks. The results show that EPO can significantly decrease the ABR thresholds in the Cdh23(erl/erl) mice as compared with those of the untreated mice at stimulus frequencies of click, 8-, 16- and 32-kHz at three time points. Meanwhile, DPOAE amplitudes in the EPO-treated Cdh23(erl/erl) mouse group were significantly higher than those of the untreated groups at f2 frequency of 15383 Hz at the three time points. Furthermore, the mean percentage of outer hair cell loss at middle through basal turns of cochleae was significantly lower in EPO-treated Cdh23(erl/erl) mice than in the untreated mice (P<0.05). This is the first report that EPO acts as an otoprotectant in a DFNB12 mouse model with progressive hearing loss.
authors: Transformative Otology and Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Yantai 264003, Shandong, PR China.
authors: F
authors: F
authors: Han
authors: H
authors: H
authors: Yu
authors: T
authors: T
authors: Zheng
authors: X
authors: X
authors: Ma
authors: X
authors: X
authors: Zhao
authors: P
authors: P
authors: Li
authors: L
authors: L
authors: Le
authors: Y
authors: Y
authors: Su
authors: Q Y
authors: QY
authors: Zheng
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000161:Acoustic Stimulation; D000704:Analysis of Variance; D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects / Q000502:physiology; D015820:Cadherins / Q000235:genetics*; D002452:Cell Count; D016009:Chi-Square Distribution; D003051:Cochlea / Q000166:cytology / Q000187:drug effects; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000627:therapeutic use*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects / Q000235:genetics; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000378:metabolism; D006319:Hearing Loss, Sensorineural / Q000188:drug therapy* / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics*; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects / Q000235:genetics; D013997:Time Factors
chemical_list: D015820:Cadherins; C499091:Cdh23 protein, mouse; D015102:3,4-Dihydroxyphenylacetic Acid; D004921:Erythropoietin
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