title: RNA "traffic lights": an analytical tool to monitor siRNA integrity.
abstract: The combination of thiazole orange and thiazole red as an internal energy transfer-based fluorophore pair in oligonucleotides provides an outstanding analytical tool to follow DNA/RNA hybridization through a distinct fluorescence color change from red to green. Herein, we demonstrate that this concept can be applied to small interfering RNA (siRNA) to monitor RNA integrity in living cells in real time with a remarkable dynamic range and excellent contrast ratios in cellular media. Furthermore, we show that our siRNA-sensors still possess their gene silencing function toward the knockdown of enhanced green fluorescent protein in CHO-K1 cells.
authors: Carolin
authors: C
authors: Holzhauser
authors: Renate
authors: R
authors: Liebl
authors: Achim
authors: A
authors: Goepferich
authors: Hans-Achim
authors: HA
authors: Wagenknecht
authors: Miriam
authors: M
authors: Breunig
mesh_terms: D000818:Animals; D001483:Base Sequence; D052160:Benzothiazoles / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D016466:CHO Cells; D002232:Carbocyanines / Q000032:analysis; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D009693:Nucleic Acid Hybridization / Q000379:methods; D011804:Quinolines / Q000032:analysis / Q000737:chemistry; D034741:RNA, Small Interfering / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011804:Quinolines; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; C584189:thiazole red; C051000:thiazole orange; D049452:Green Fluorescent Proteins