title: Determination of monosaccharides derivatized with 2-aminobenzoic Acid by capillary electrophoresis.
abstract: Reducing monosaccharides were derivatized with 2-aminobenzoic acid (2-AA) through reductive amination using sodium cyanoborohydride as a reductant, and the derivatives were separated by capillary zone electrophoresis with UV detection using 50 mM sodium phosphate (pH 5.5) or 150 mM sodium borate-50 mM sodium phosphate (pH 7.0) running buffer. The derivatives of monosaccharides, which are major components of various carbohydrate materials, were completely separated within 25 min.
authors: Yokohama College of Pharmacy, Yokohama, Japan. abo@mail.ecc.u-tokio.ac.jp
authors: Mitsuru
authors: M
authors: Abo
authors: Li-Ping
authors: LP
authors: He
authors: Kae
authors: K
authors: Sato
authors: Akira
authors: A
authors: Okubo
mesh_terms: D000586:Amination; D001881:Borates / Q000737:chemistry; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D009005:Monosaccharides / Q000138:chemical synthesis / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D010710:Phosphates / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D001881:Borates; D002021:Buffers; D009005:Monosaccharides; D010710:Phosphates; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; C010634:sodium borate; C018279:sodium phosphate