title: Generation and characterization of pan-specific anti-acetyllysine antibody.
abstract: Pan-specific anti-acetylated lysine antibody is a valuable tool to detect, validate, and quantify protein lysine acetylation. Compared to site-specific acetyllysine antibodies that are not readily available in most cases, polyclonal pan-specific acetyllysine antibodies can be generated with synthetic antigen of a carrier protein that has its lysine residues chemically acetylated. Here, we describe protocols of synthesizing acetylated ovalbumin (OVA), immunizing rabbits, and characterizing pan-specific polyclonal anti-acetylated lysine antibodies.
authors: State Key Lab of Genetic Engineering, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors: Wei
authors: W
authors: Xu
authors: Shimin
authors: S
authors: Zhao
mesh_terms: D000107:Acetylation; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D000915:Antibody Affinity; D000918:Antibody Specificity; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D006657:Histones / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology*; D011499:Protein Processing, Post-Translational; D011817:Rabbits
chemical_list: D000906:Antibodies; D000941:Antigens; D006657:Histones; D010047:Ovalbumin; D008239:Lysine