title: Detection of necrosis by release of lactate dehydrogenase activity.
abstract: Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.
authors: Immunology and Virology Program, Department of Pathology, Center for AIDS Research, University of Massachusetts Medical School, Worcester, MA, USA. Francis.Chan@umassmed.edu
authors: Francis Ka-Ming
authors: FK
authors: Chan
authors: Kenta
authors: K
authors: Moriwaki
authors: María José
authors: MJ
authors: De Rosa
mesh_terms: D055162:Biocatalysis; D018929:Cell Culture Techniques; D003124:Colorimetry / Q000379:methods*; D004579:Electron Transport; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009336:Necrosis / Q000201:enzymology*; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D013778:Tetrazolium Salts; D009243:NAD; D007770:L-Lactate Dehydrogenase
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