title: Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients.
abstract: An automated microfluidic cell culture platform that creates and maintains independently programmable diffusion-based gradients is reported. Temporal modulation of the source and sink flow patterns allow generation of extremely stable spatial gradients. We developed a system that integrates 30 parallel gradients in a single device, with 10 different chemical formulations and 3 replicates. Mammalian fibroblast and macrophage cells were screened for NFκB pathway activity under gradients of TNFα, PDGF, and LPS, and multiparameter measurements were performed to demonstrate the capability of the device in dynamic single-cell analysis.
authors: ETH Zurich, Department of Biosystems Science and Engineering, Mattenstrasse 26, 4058 Basel, Switzerland.
authors: Tino
authors: T
authors: Frank
authors: Savaş
authors: S
authors: Tay
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D004058:Diffusion; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D010982:Platelet-Derived Growth Factor; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha