title: Mapping the apoptosis inducing domain of an immunomodulatory protein: glycodelin A.
abstract: Glycodelin A (GdA) is a dimeric glycoprotein synthesized by the human endometrium under progesterone regulation. Based on the high sequence similarity with β-lactoglobulin, it is placed under the lipocalin superfamily. The protein is one of the local immunomodulators present at the feto-maternal interface which affects both the innate as well as the acquired arms of the immune system, thereby bringing about successful establishment and progression of pregnancy. Our previous studies revealed that the domain responsible for the immunosuppressive activity of glycodelin lies on its protein backbone and the glycans modulate the same. This study attempts to further delineate the apoptosis inducing region of GdA. Our results demonstrate that the stretch of amino acid sequence between Met24 to Leu105 is necessary and sufficient to inhibit proliferation of T cells and induce apoptosis in them. Further, within this region the key residues involved in harboring the activity were shown to be present between Asp52 and Ser65.
authors: Department of Biochemistry, Indian Institute of Science, Bangalore, India.
authors: Devasena
authors: D
authors: Ponnalagu
authors: Anjali A
authors: AA
authors: Karande
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D017209:Apoptosis; D016367:Baculoviridae / Q000235:genetics; D049109:Cell Proliferation; D005822:Genetic Vectors; D000071816:Glycodelin; D006023:Glycoproteins / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000502:physiology*; D019169:Jurkat Cells; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010449:Peptide Mapping; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D061987:Sf9 Cells; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; D007155:Immunologic Factors; C045246:PAEP protein, human
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