title: Grafted megaporous materials as ion-exchangers for bioproduct adsorption.
abstract: Megaporous chromatographic materials were manufactured by a three-step procedure, including backbone synthesis, chemical grafting, and introduction of ion-exchange functionality. The backbone of the adsorbent cylindrical bodies was prepared by polymerization of methacrylic acid and poly(ethylene glycol) diacrylate at sub-zero temperatures. Grafting was performed employing glycidyl methacrylate and a chemical initiator, cerium ammonium nitrate. The degree of grafting was adjusted by modifying the concentration of the initiator in the reaction mixture to a range of values (23, 39, 62, 89, and 105%). Further, the pendant epoxy-groups generated by the previous step were reacted to cation- and anion-exchanging moieties utilizing known chemical routes. Infrared spectroscopy studies confirmed the incorporation of epoxy and ion-exchanger groups to the backbone material. Optimized materials were tested for chromatography applications with model proteins; the dynamic binding capacity, as recorded at 10% breakthrough and 2.0 × 10(-4) m/s superficial velocity, were 350 and 58 mg/g for the cation-exchanger and the anion-exchanger material, respectively. These results may indicate that long tentacle-type polymer brushes were formed during grafting therefore increasing the ability of the megaporous body to efficiently capture macromolecules.
authors: Downstream BioProcessing Laboratory, School of Engineering and Science, Jacobs University, Campus Ring 1, D-28759, Bremen, Germany.
authors: Noor Shad
authors: NS
authors: Bibi
authors: Marcelo
authors: M
authors: Fernández-Lahore
mesh_terms: D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D007475:Ion Exchange Resins / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000737:chemistry*
chemical_list: D007475:Ion Exchange Resins; D011108:Polymers; D011506:Proteins