title: Range-reference determination of lymphocyte subsets in Moroccan blood donors.
abstract: BACKGROUND
  Information on lymphocyte populations (T, B, and Natural killer cells) and subpopulations (CD4 and CD8) in Morocco is scarce if not inexistent.
  
  
  OBJECTIVE
  To establish a reference value of these cells in 242 Moroccan young adult blood donors by flow cytometry.
  
  
  RESULTS
  Smokers had significantly higher total leukocyte count (p < 0.001), total lymphocyte count (p < 0.0001) and higher CD3+CD4+ cells (p < 0.0001). The percentage of CD3-CD56+ subsets was affected by smoking (p < 0.01). Our analysis positively correlate with previous observations of an increase of absolute CD4+ T cells, with no changes in other lymphocyte subset cells in smokers. The lymphocyte subpopulation distributions for all antigens were found to be similar to those reported in Saudi and Italian adults, while higher levels were reported for the same gender in other countries, especially Ghana and Kuwait.
  
  
  CONCLUSIONS
  The international classification standards of the HIV-infected subjects according to their rates of CD4 are applicable to the present study's population.
authors: Laboratoire de Virologie, Hôpital Militaire d'Instruction Med V Rabat, Um5, Souissi, Morocco. ntouil2003@gmail.com
authors: N
authors: N
authors: Touil
authors: R
authors: R
authors: Hadef
authors: A
authors: A
authors: Lemnouer
authors: A
authors: A
authors: Zrara
authors: A I
authors: AI
authors: Sbai
authors: B
authors: B
authors: Belfquih
authors: S
authors: S
authors: Mrani
authors: A
authors: A
authors: Benkirane
authors: M
authors: M
authors: Ouaaline
authors: M
authors: M
authors: Mrabet
mesh_terms: D000328:Adult; D017677:Age Distribution; D000704:Analysis of Variance; D001782:Blood Donors; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009018:Morocco; D012016:Reference Values; D017678:Sex Distribution; D012907:Smoking / Q000009:adverse effects / Q000276:immunology*; D018709:Statistics, Nonparametric
keywords: Lymphocyte counts; Young Moroccan adults; cigarette effects; flow cytometry
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