title: Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells.
abstract: A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strains.
authors: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, 2-24-16 Naka-cho, Koganei, Tokyo, Japan. mhide@cc.tuat.ac.jp
authors: Hideaki
authors: H
authors: Matsuoka
authors: Tomoya
authors: T
authors: Shigetomi
authors: Hisakage
authors: H
authors: Funabashi
authors: Mikako
authors: M
authors: Saito
authors: Shizunobu
authors: S
authors: Igimi
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005516:Food Microbiology / Q000379:methods*; D050296:Microbial Viability; D013194:Staining and Labeling
chemical_list: D003470:Culture Media; D005452:Fluoresceins; D005456:Fluorescent Dyes; C093616:6-carboxyfluorescein diacetate