title: Identification of QTLs associated with callogenesis and embryogenesis in oil palm using genetic linkage maps improved with SSR markers.
abstract: Clonal reproduction of oil palm by means of tissue culture is a very inefficient process. Tissue culturability is known to be genotype dependent with some genotypes being more amenable to tissue culture than others. In this study, genetic linkage maps enriched with simple sequence repeat (SSR) markers were developed for dura (ENL48) and pisifera (ML161), the two fruit forms of oil palm, Elaeis guineensis. The SSR markers were mapped onto earlier reported parental maps based on amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) markers. The new linkage map of ENL48 contains 148 markers (33 AFLPs, 38 RFLPs and 77 SSRs) in 23 linkage groups (LGs), covering a total map length of 798.0 cM. The ML161 map contains 240 markers (50 AFLPs, 71 RFLPs and 119 SSRs) in 24 LGs covering a total of 1,328.1 cM. Using the improved maps, two quantitative trait loci (QTLs) associated with tissue culturability were identified each for callusing rate and embryogenesis rate. A QTL for callogenesis was identified in LGD4b of ENL48 and explained 17.5% of the phenotypic variation. For embryogenesis rate, a QTL was detected on LGP16b in ML161 and explained 20.1% of the variation. This study is the first attempt to identify QTL associated with tissue culture amenity in oil palm which is an important step towards understanding the molecular processes underlying clonal regeneration of oil palm.
authors: Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board, Kajang, Selangor, Malaysia.
authors: Ngoot-Chin
authors: NC
authors: Ting
authors: Johannes
authors: J
authors: Jansen
authors: Jayanthi
authors: J
authors: Nagappan
authors: Zamzuri
authors: Z
authors: Ishak
authors: Cheuk-Weng
authors: CW
authors: Chin
authors: Soon-Guan
authors: SG
authors: Tan
authors: Suan-Choo
authors: SC
authors: Cheah
authors: Rajinder
authors: R
authors: Singh
mesh_terms: D028023:Arecaceae / Q000235:genetics; D002874:Chromosome Mapping; D018895:Microsatellite Repeats / Q000235:genetics*; D059828:Plant Cells / Q000502:physiology; D063245:Plant Development / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D012639:Seeds / Q000235:genetics* / Q000254:growth & development
references: Theor Appl Genet. 2002 Oct;105(5):708-715
references: 12582484
references: Theor Appl Genet. 2010 May;120(8):1673-87
references: 20182696
references: Heredity (Edinb). 2005 Sep;95(3):221-7
references: 16077740
references: Theor Appl Genet. 2006 Sep;113(5):821-30
references: 16896717
references: J Genet. 2010 Aug;89(2):135-45
references: 20861564
references: Cytometry A. 2003 Feb;51(2):127-8; author reply 129
references: 12541287
references: Theor Appl Genet. 1993 Jul;86(6):713-20
references: 24193781
references: Genetics. 1994 Aug;137(4):1121-37
references: 7982566
references: J Hered. 2002 Jan-Feb;93(1):77-8
references: 12011185
references: Theor Appl Genet. 1980 Nov;58(6):265-71
references: 24301504
references: Biotechniques. 2001 Jul;31(1):24-6, 28
references: 11464515
references: BMC Plant Biol. 2008 May 29;8:62
references: 18507865
references: Cell Mol Biol Lett. 2007;12(4):523-35
references: 17579815
references: Theor Appl Genet. 2005 Feb;110(4):754-65
references: 15723275
references: BMC Plant Biol. 2009 Aug 26;9:114
references: 19706196
references: Genetics. 2005 Aug;170(4):2013-25
references: 15944349