title: Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain.
abstract: Radiotherapy is one of the major treatment modalities for malignancies. However, cells surviving irradiation often display high levels of invasiveness. This study shows that irradiation-tolerant lung adenocarcinoma demonstrates high invasive capability depending on dephosphorylation of the myosin regulatory light chain (MRLC). In a collagen gel overlay condition, low-invasive subclones of lung adenocarcinoma (A549P-3) showed a round morphology and diphosphorylation of MRLC. In contrast, irradiation-tolerant A549P-3 cells (A549P-3IR) displayed high invasiveness and a lower level of MRLC diphosphorylation. In addition, inhibition of MRLC phosphatase activity decreased the invasive activity. These findings suggest that A549P-3IR cells acquire high invasiveness through MRLC dephosphorylation.
authors: Transdisciplinary Life Science Course, Faculty of Advanced Life Science, Hokkaido University, N10-W8, Kita-ku, Sapporo 060-0810, Japan.
authors: Seiichiro
authors: S
authors: Ishihara
authors: Motoaki
authors: M
authors: Yasuda
authors: Takeshi
authors: T
authors: Nishioka
authors: Takeomi
authors: T
authors: Mizutani
authors: Kazushige
authors: K
authors: Kawabata
authors: Hiroki
authors: H
authors: Shirato
authors: Hisashi
authors: H
authors: Haga
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy; D000077192:Adenocarcinoma of Lung; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000528:radiation effects; D003094:Collagen; D005720:Gamma Rays; D005782:Gels; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy; D018994:Myosin Light Chains / Q000235:genetics / Q000378:metabolism*; D043263:Myosin-Light-Chain Phosphatase / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D010766:Phosphorylation; D011836:Radiation Tolerance / Q000235:genetics*
chemical_list: D005782:Gels; D018994:Myosin Light Chains; D003094:Collagen; D043263:Myosin-Light-Chain Phosphatase