title: Fast and interference-free determination of glyphosate and glufosinate residues through electrophoresis in disposable microfluidic chips.
abstract: With an increasing concern on food safety, fast screening of residues of widespread herbicides becomes necessary. Herein we report a microchip electrophoresis system with laser induced fluorescence (LIF) detection for rapid and sensitive analysis of glyphosate (GLYP) and glufosinate (GLUF) residues. Disposable cyclic olefin copolymer microchips and a low-cost LIF detector were employed to minimize the cost of the analysis. Systematic optimization of experimental conditions was performed to achieve highly efficient analysis. Under the selected condition, GLYP and GLUF were efficiently resolved from sample matrices with a buffer containing 10 mmol/L borax and 2.0% (m/v) hydroxypropyl cellulose at pH 9.0. The number of theoretical plates of 1.0×10(6) m(-1) was attained for both analytes. Derivatization at lower concentrations (<10 μg/L) was also examined, successful detection of 0.34 μg/L GLYP and 0.18 μg/L GLUF was confirmed. The system was applied for the determination of both analytes in real samples without any preconcentration involved. Recoveries of GLYP and GLUF spiked in these samples were 84.0-101.0% and 90.0-103.0%, respectively.
authors: State Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors: Xuan
authors: X
authors: Wei
authors: Xiaotong
authors: X
authors: Gao
authors: Lei
authors: L
authors: Zhao
authors: Xianglu
authors: X
authors: Peng
authors: Lei
authors: L
authors: Zhou
authors: Jing
authors: J
authors: Wang
authors: Qiaosheng
authors: Q
authors: Pu
mesh_terms: D000613:Aminobutyrates / Q000032:analysis* / Q000737:chemistry; D001881:Borates / Q000737:chemistry; D001937:Brassica / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D006540:Herbicides / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D012044:Regression Analysis; D015203:Reproducibility of Results; D013025:Glycine max / Q000737:chemistry; D000097797:Glyphosate
chemical_list: D000613:Aminobutyrates; D001881:Borates; D005998:Glycine; D006540:Herbicides; D010573:Pesticide Residues; D000097797:Glyphosate; C018851:borax; C003121:phosphinothricin