title: Anti-phosphohistone H3 as an independent prognostic factor in human esophageal squamous cell carcinoma.
abstract: BACKGROUND
  The immunohistochemical staining of phospho-histone H3 (PHH3) has recently been reported to predict the prognosis of different tumors. However, it has not been evaluated in esophageal squamous cell carcinoma (ESCC). The aim of this study was to evaluate the prognostic impact of PHH3 in ESCC.
  
  
  METHODS
  The number of anti-phophohistone H3-positive nuclei [i.e. PHH3 mitotic index (MI)] was calculated by immunohistochemistry of 50 primary tumor samples obtained from patients with ESCC who underwent curative esophagectomy.
  
  
  RESULTS
  The PHH3 MI per 10 high-power fields ranged from 1 to 72 (median=15.5). When the patients were divided into two groups using a cut-off value of 10, the 5-year survival rate of the patients with PHH3 MI ≤10 was significantly higher than that of patients with PHH3 MI >10. Multivariate analysis indicated PHH3 MI to be an independent prognostic factor.
  
  
  CONCLUSIONS
  The expression of PHH3 impacts the prognosis of patients with ESCC.
authors: Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.
authors: Shingo
authors: S
authors: Nakashima
authors: Atsushi
authors: A
authors: Shiozaki
authors: Daisuke
authors: D
authors: Ichikawa
authors: Shuhei
authors: S
authors: Komatsu
authors: Hirotaka
authors: H
authors: Konishi
authors: Daisuke
authors: D
authors: Iitaka
authors: Takeshi
authors: T
authors: Kubota
authors: Hitoshi
authors: H
authors: Fujiwara
authors: Kazuma
authors: K
authors: Okamoto
authors: Mitsuo
authors: M
authors: Kishimoto
authors: Eigo
authors: E
authors: Otsuji
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D010766:Phosphorylation; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D014408:Biomarkers, Tumor; D006657:Histones