title: Molecular characterization of sexual diversity in a population of Serpula lacrymans, a tetrapolar basidiomycete.
abstract: Different mating systems have evolved in the fungal kingdom, including a tetrapolar multiallelic mating system in many basidiomycetes. In tetrapolar species, the presence of different alleles at two mating loci (MAT A and MAT B) is necessary for mating to occur. The tetrapolar fungus Serpula lacrymans causes wood-decay in buildings in temperate regions worldwide and is present in Europe with a genetically homogeneous founder population. Using genome sequence data, we annotated the two mating type loci for S. lacrymans and found the expected synteny with other basidiomycetes, except for a retrotransposon being present in one locus (MAT A). We developed markers linked to the MAT A and MAT B regions and used these to investigate the mating type diversity in the European population. Moreover, we found a good match between the genetic markers and functional mating types as revealed by segregation and mating studies. A low diversity of mating types is present in the European S. lacrymans population caused by the founder event where a limited number of genotypes were introduced. This finding contrasts the situation in natural fungal populations where a high diversity of mating types is normally present. Although S. lacrymans has a large and viable population in Europe, we argue that the low mating type diversity restrains the dispersal and establishment of the fungus.
authors: Microbial Evolution Research Group, Department of Biology, University of Oslo, N-0316 Oslo, Norway. inger.skrede@bio.uio.no
authors: Inger
authors: I
authors: Skrede
authors: Sundy
authors: S
authors: Maurice
authors: Håvard
authors: H
authors: Kauserud
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics* / Q000502:physiology; D030541:Databases, Genetic; D049770:Genes, Mating Type, Fungal; D056426:Genetic Loci; D016681:Genome, Fungal; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D013172:Spores, Fungal / Q000235:genetics
keywords: dry rot; fungus; mating type; population diversity; tetrapolar
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