title: Hollow nanosphere fabricated from β-cyclodextrin-grafted α,β-poly(aspartic acid) as the carrier of camptothecin.
abstract: This research is aimed to develop a kind of hollow nanosphere based on β-cyclodextrin-grafted α, β-poly(aspartic acid) (β-CD-graft-PAsp) as drug carrier to enhance the stability of camptothecin (CPT) in aqueous media. Firstly, mono(6-(2-aminoethyl) amino-6-deoxy)-β-cyclodextrin (β-CDen) was synthesized by a substitution reaction between mono-6-deoxy-6-(p-tolylsulfonyl)-β-cyclodextrin (6-OTs-β-CD) and ethylenediamine; and then, the five-member rings in poly(L-succinimide) (PSI) were successively opened by β-CDen to obtain β-CD-graft-PAsp. The synthesized β-CD-graft-PAsp was found to form the unique hollow nanosphere with the internal hole of about 17 nm and many β-CD cavities of 0.7 nm interspersed on the shell. The drug-loading behavior of the hollow nanosphere was also evaluated by using CPT as guest molecule of β-CD. It was worth of note that the β-CD-graft-PAsp hollow nanosphere as carrier enhanced the stability of CPT in aqueous media, and the CPT from the β-CD-graft-PAsp hollow nanosphere displayed a sustained release profile and hence resulted in the essential decrease of cytotoxicity to L929 cell line. Furthermore, almost no cytotoxicity of the β-CD-graft-PAsp is desirable for the application of drug carrier. As a result, the β-CD-graft-PAsp hollow nanosphere showed a great potential as the carrier of CPT.
authors: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, PR China.
authors: Junping
authors: J
authors: Zeng
authors: Haitao
authors: H
authors: Huang
authors: Shiyuan
authors: S
authors: Liu
authors: Haibo
authors: H
authors: Xu
authors: Jin
authors: J
authors: Huang
authors: Jiahui
authors: J
authors: Yu
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D001224:Aspartic Acid / Q000737:chemistry* / Q000378:metabolism; D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism; D002166:Camptothecin / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004337:Drug Carriers; D004339:Drug Compounding; D005354:Fibrosarcoma / Q000188:drug therapy*; D051379:Mice; D054118:Nanospheres / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D014867:Water / Q000737:chemistry / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001704:Biopolymers; D004337:Drug Carriers; C499773:alpha,beta-poly(aspartic acid); D047392:beta-Cyclodextrins; D014867:Water; D001224:Aspartic Acid; C031215:betadex; D002166:Camptothecin