title: Col-F, a fluorescent probe for ex vivo confocal imaging of collagen and elastin in animal tissues.
abstract: A new low-molecular-weight fluorescent probe, Col-F, that exhibits affinity to collagen and elastin, was used successfully in imaging of extracellular matrix in freshly excised animal tissues. Col-F readily penetrates between live cells into tissues and binds to fibers of collagen and elastin by a noncovalent mechanism. Fibers of collagen and elastin have been stained in a variety of tissues, including tendon, skeletal muscle, connective tissue, and arteries. Cells migrating in a Col-F-stained collagenous biomaterial were also imaged. No phototoxic effects were detected when live keratocytes were imaged in the in vitro culture in the presence of Col-F. In conclusion, Col-F provides a simple and convenient tool for fluorescence three-dimensional imaging of intricate collagenous and elastic structures in live and fixed animal tissues, as well as in collagen-containing biomaterials.
authors: Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Kraków, Poland.
authors: Ewa
authors: E
authors: Biela
authors: Jerzy
authors: J
authors: Galas
authors: Brian
authors: B
authors: Lee
authors: Gary L
authors: GL
authors: Johnson
authors: Zbigniew
authors: Z
authors: Darzynkiewicz
authors: Jurek W
authors: JW
authors: Dobrucki
mesh_terms: D000818:Animals; D001158:Arteries / Q000737:chemistry / Q000648:ultrastructure; D001692:Biological Transport; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure*; D003238:Connective Tissue / Q000737:chemistry / Q000648:ultrastructure; D004549:Elastin / Q000737:chemistry / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure*; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000737:chemistry / Q000648:ultrastructure; D010830:Physostigmine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D013710:Tendons / Q000737:chemistry / Q000648:ultrastructure
chemical_list: C585053:Col-F compound; D005452:Fluoresceins; D005456:Fluorescent Dyes; D003094:Collagen; D004549:Elastin; D010830:Physostigmine
references: Circ Res. 1964 May;14:400-13
references: 14156860
references: Biophys J. 2005 Feb;88(2):1377-86
references: 15533922
references: Tissue Eng Part C Methods. 2010 Aug;16(4):705-10
references: 19803792
references: Nat Biotechnol. 2003 Nov;21(11):1356-60
references: 14595363
references: Invest Ophthalmol Vis Sci. 2006 Mar;47(3):1216-24
references: 16505061
references: Methods Cell Biol. 2001;63:583-97
references: 11060860
references: J Biomed Opt. 2007 Nov-Dec;12(6):064017
references: 18163833
references: Biochimie. 2005 Mar-Apr;87(3-4):315-20
references: 15781318
references: Birth Defects Res C Embryo Today. 2004 Mar;72(1):1-11
references: 15054900
references: Cardiovasc Pathol. 2005 Jul-Aug;14(4):185-8
references: 16009316
references: Cytometry A. 2010 Apr;77(4):366-70
references: 20131402
references: J Anat. 2009 Dec;215(6):682-91
references: 19796069
references: Biopolymers. 2000 Sep;54(3):222-34
references: 10861383
references: Arch Pathol. 1964 Jul;78:69-72
references: 14150734
references: Matrix Biol. 2008 Apr;27(3):171-81
references: 18248974
references: Front Biosci. 2006 Jan 01;11:529-43
references: 16146751
references: Appl Opt. 1971 Oct 1;10(10):2350-3
references: 20111328
references: Scanning. 2006 Nov-Dec;28(6):305-10
references: 17181131
references: Circ Res. 1967 Jan;20(1):99-111
references: 4959753
references: Cell Cycle. 2005 Jan;4(1):140-7
references: 15611638
references: Adv Protein Chem. 2005;70:341-74
references: 15837520
references: Histochemistry. 1979 Nov;64(2):119-30
references: 93591
references: Anal Biochem. 2006 Mar 15;350(2):177-85
references: 16476406
references: Biophys J. 1976 Sep;16(9):1055-69
references: 786399
references: Cardiovasc Res. 2006 Jul 1;71(1):40-9
references: 16566911
references: Biomed Mater Eng. 2005;15(6):413-20
references: 16308457
references: Biochem J. 1996 May 15;316 ( Pt 1):1-11
references: 8645190
references: Histochem J. 1990 Sep;22(9):487-90
references: 1702087