title: Localized in situ hydrogel-mediated protein digestion and extraction technique for on-tissue analysis.
abstract: A simultaneous on-tissue proteolytic digestion and extraction method is described for the in situ analysis of proteins from spatially distinct areas of a tissue section. The digestion occurs on-tissue within a hydrogel network, and peptides extracted from this gel are identified with liquid chromatography tandem MS (LC-MS/MS). The hydrogels are compatible with solubility agents (e.g., chaotropes and detergents) known to improve enzymatic digestion of proteins. Additionally, digestions and extractions are compatible with imaging mass spectrometry (IMS) experiments. As an example application, an initial IMS experiment was conducted to profile lipid species using a traveling wave ion mobility mass spectrometer. On-tissue MS/MS was also performed on the same tissue section to identify lipid ions that showed spatial differences. Subsequently, the section underwent an on-tissue hydrogel digestion to reveal 96 proteins that colocalized to the rat brain cerebellum. Hematoxylin and eosin (H & E) staining was then performed to provide additional histological information about the tissue structure. This technology provides a versatile workflow that can be used to correlate multiple complementary analytical approaches in the analysis of a single tissue section.
authors: Department of Biochemistry and the Mass Spectrometry Research Center, Vanderbilt University, 9160 MRB3, 465 21st Avenue South, Nashville, Tennessee 37235, United States.
authors: Glenn A
authors: GA
authors: Harris
authors: Joshua J
authors: JJ
authors: Nicklay
authors: Richard M
authors: RM
authors: Caprioli
mesh_terms: D000818:Animals; D054022:Cerebrum / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002853:Chromatography, Liquid; D020100:Hydrogels / Q000737:chemistry*; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D059748:Proteolysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D020100:Hydrogels; D011506:Proteins
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