title: Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook.
abstract: BACKGROUND
  Potentilla fulgens (Wall.) ex Hook. (Rosaceae) is a potent medicinal plant of the Western Himalayas, known under the name "Himalayan Cinquefoil or Bajradanti", and has been used traditionally to treat ailments including peptic ulcers, mouth ulcers, diarrhea, diabetes and cancer.
  
  
  OBJECTIVE
  The aim of the present study was to scientifically evaluate the gastric-ulcer protective effect of P. fulgens ethanolic root extract (EPF) on experimental rats.
  
  
  METHODS
  The gastroprotective activity of EPF was evaluated on four gastric-ulcer models such as pyloric ligation (PL), ethanol (EtOH), cold restrain stress (CRS) and aspirin (ASP)-induced gastric ulcers. The gastric acid obtained from 4h PL-induced gastric ulcer rats was determined for total volume content, pH and total acid-pepsin output. Total carbohydrates and protein ratio, expressed as index of mucin activity, and DNA content were estimated in the gastric juice and gastric mucosal tissue. The microvascular permeability, H(+)K(+)-ATPase activity, gastric mucus and histamine content were also determined. The levels of antioxidant enzymes (superoxide dismutase, catalase, and glutathione) and malondialdehyde in the stomach tissue (mucosal scrapings) were quantified. A histopathological study of the stomach was evaluated using eosin-haematoxylin stain.
  
  
  RESULTS
  EPF (200-400mg/kg, p.o.) showed significant protection against acute gastric-ulcer induced by EtOH, PL and CRS (400mg/kg, p.o.), but was found to be ineffective against ASP-induced ulcerogens. The effect of EPF on gastric juice studies in 4h PL rats significantly produced an increased level in gastric pH, whereas the effect on gastric volume and acid-pepsin output was observed to decrease significantly. However, EPF was found to have no significant effect on the defensive factors, thus revealing its antisecretory property by inhibiting the aggressive factors. EPF, significantly decreased the histamine level, inhibited the H(+)K(+)-ATPase activity and prevented the microvascular injury caused by ethanol in the rat stomach. Moreover, it was also observed to have antioxidant effects by producing a significant increase in the levels of SOD, CAT, and GSH and decreased the LPO activity. Histopathological studies showed that EPF significantly prevented gastric lesions caused by ethanol.
  
  
  CONCLUSIONS
  The present study showed that EPF has potent gastroprotective and antisecretory effects, thus justifying the traditional usage of this herb to treat gastric ulcers.
authors: Department of Pharmaceutics, Indian Institute of Technology, Banaras Hindu University, Varanasi 221 005, India.
authors: Damiki
authors: D
authors: Laloo
authors: Satyendra K
authors: SK
authors: Prasad
authors: Sairam
authors: S
authors: Krishnamurthy
authors: Siva
authors: S
authors: Hemalatha
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001241:Aspirin; D002374:Catalase / Q000378:metabolism; D003080:Cold Temperature; D004247:DNA / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006632:Histamine / Q000378:metabolism; D008297:Male; D009093:Mucus / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots; D031987:Potentilla; D051381:Rats; D011919:Rats, Inbred Strains; D012997:Solvents / Q000737:chemistry; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D006023:Glycoproteins; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; D006632:Histamine; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D001241:Aspirin