title: Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer.
abstract: We reported earlier the delivery of antiangiogenic single chain antibodies by using oncolytic vaccinia virus strains to enhance their therapeutic efficacy. Here, we provide evidence that gene-evoked production of melanin can be used as a therapeutic and diagnostic mediator, as exemplified by insertion of only one or two genes into the genome of an oncolytic vaccinia virus strain. We found that produced melanin is an excellent reporter for optical imaging without addition of substrate. Melanin production also facilitated deep tissue optoacoustic imaging as well as MRI. In addition, melanin was shown to be a suitable target for laser-induced thermotherapy and enhanced oncolytic viral therapy. In conclusion, melanin as a mediator for thermotherapy and reporter for different imaging modalities may soon become a versatile alternative to replace fluorescent proteins also in other biological systems. After ongoing extensive preclinical studies, melanin overproducing oncolytic virus strains might be used in clinical trials in patients with cancer.
authors: Genelux, San Diego, CA 92109, USA. js@genelux.com
authors: Jochen
authors: J
authors: Stritzker
authors: Lorenz
authors: L
authors: Kirscher
authors: Miriam
authors: M
authors: Scadeng
authors: Nikolaos C
authors: NC
authors: Deliolanis
authors: Stefan
authors: S
authors: Morscher
authors: Panagiotis
authors: P
authors: Symvoulidis
authors: Karin
authors: K
authors: Schaefer
authors: Qian
authors: Q
authors: Zhang
authors: Lisa
authors: L
authors: Buckel
authors: Michael
authors: M
authors: Hess
authors: Ulrike
authors: U
authors: Donat
authors: William G
authors: WG
authors: Bradley
authors: Vasilis
authors: V
authors: Ntziachristos
authors: Aladar A
authors: AA
authors: Szalay
mesh_terms: D000818:Animals; D006367:HeLa Cells; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007259:Infrared Rays; D007834:Lasers; D008279:Magnetic Resonance Imaging; D008543:Melanins / Q000096:biosynthesis*; D051379:Mice; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D061088:Photoacoustic Techniques / Q000379:methods*; D014616:Vaccinia virus / Q000378:metabolism*
chemical_list: D008543:Melanins
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