title: Developmental genetics of the perianthless flowers and bracts of a paleoherb species, Saururus chinensis.
abstract: Saururus chinensis is a core member of Saururaceae, a perianthless (lacking petals or sepals) family. Due to its basal phylogenetic position and unusual floral composition, study of this plant family is important for understanding the origin and evolution of perianthless flowers and petaloid bracts among angiosperm species. To isolate genes involved in S. chinensis flower development, subtracted floral cDNA libraries were constructed by using suppression subtractive hybridization (SSH) on transcripts isolated from developing inflorescences and seedling leaves. The subtracted cDNA libraries contained a total of 1,141 ESTs and were used to create cDNA microarrays to analyze transcript profiles of developing inflorescence tissues. Subsequently, qRT-PCR analyses of eight MADS-box transcription factors and in situ hybridizations of two B-class MADS-box transcription factors were performed to verify and extend the cDNA microarray results. Finally, putative phylogenetic relationships within the B-class MADS-box gene family were determined using the discovered S. chinensis B-class genes to compare K-domain sequences with B genes from other basal angiosperms. Two hundred seventy-seven of the 1,141 genes were found to be expressed differentially between S. chinensis inflorescence tissues and seedling leaves, 176 of which were grouped into at least one functional category, including transcription (14.75%), energy (12.59%), metabolism (9.12%), protein-related function (8.99%), and cellular transport (5.76%). qRT-PCR and in situ hybridization of selected MADS-box genes supported our microarray data. Phylogenetic analysis indicated that a total of six B-class MADS-box genes were isolated from S. chinensis. The differential regulation of S. chinensis B-class MADS-box transcription factors likely plays a role during the development of subtending bracts and perianthless flowers. This study contributes to our understanding of inflorescence development in Saururus, and represents an initial step toward understanding the formation of petaloid bracts in this species.
authors: Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
authors: Yin-He
authors: YH
authors: Zhao
authors: Zachary
authors: Z
authors: Larson-Rabin
authors: Guo-Ying
authors: GY
authors: Wang
authors: Michael
authors: M
authors: Möller
authors: Cheng-Yun
authors: CY
authors: Li
authors: Jin-Peng
authors: JP
authors: Zhang
authors: Hong-Tao
authors: HT
authors: Li
authors: De-Zhu
authors: DZ
authors: Li
mesh_terms: D005075:Biological Evolution; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017403:In Situ Hybridization; D026161:MADS Domain Proteins / Q000235:genetics*; D019684:Magnoliopsida / Q000235:genetics*; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D032134:Saururaceae / Q000235:genetics / Q000254:growth & development
chemical_list: D026161:MADS Domain Proteins
references: Mol Biol Evol. 2004 Mar;21(3):506-19
references: 14694075
references: Evol Dev. 2004 Nov-Dec;6(6):449-58
references: 15509227
references: Ann Bot. 2007 Sep;100(3):621-30
references: 17513305
references: Ann Bot. 2004 Nov;94(5):741-51
references: 15451722
references: Plant Cell. 2010 Mar;22(3):565-78
references: 20305121
references: Plant J. 2006 May;46(3):503-11
references: 16623909
references: Evol Dev. 1999 Nov-Dec;1(3):180-90
references: 11324103
references: Plant Mol Biol. 2003 Jul;52(4):831-41
references: 13677470
references: Plant Mol Biol. 2000 Jan;42(1):115-49
references: 10688133
references: Mol Phylogenet Evol. 2002 Jun;23(3):458-80
references: 12099799
references: Cell. 1992 Feb 21;68(4):683-97
references: 1346756
references: Dev Genet. 1999 Sep;25(3):245-52
references: 10528265
references: Plant J. 2005 Sep;43(5):724-44
references: 16115069
references: Plant J. 2005 Mar;41(5):755-66
references: 15703062
references: Am J Bot. 2009 Jan;96(1):110-28
references: 21628179
references: Cell. 1994 Feb 25;76(4):703-16
references: 7907276
references: Plant Mol Biol. 2003 Nov;53(4):545-63
references: 15010618
references: Nature. 2000 May 11;405(6783):200-3
references: 10821278
references: Mol Phylogenet Evol. 2007 Aug;44(2):598-609
references: 17576077
references: Biol Chem. 1997 Oct;378(10):1079-101
references: 9372178
references: Genomics. 2007 Jul;90(1):121-31
references: 17498921
references: Mol Biol Evol. 2002 May;19(5):587-96
references: 11961093
references: Nat Rev Genet. 2002 Aug;3(8):579-88
references: 12154381
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Plant J. 2003 Jan;33(1):47-59
references: 12943540
references: Plant Mol Biol. 2005 Jun;58(3):401-19
references: 16021403
references: Plant Cell. 2007 Mar;19(3):750-66
references: 17400892
references: Nature. 2001 Jan 25;409(6819):525-9
references: 11206550
references: Genes Dev. 1991 Mar;5(3):484-95
references: 1672119
references: Ann Bot. 2006 Jul;98(1):157-63
references: 16675604
references: Plant Physiol. 2002 Mar;128(3):896-910
references: 11891246
references: Curr Biol. 2001 Feb 6;11(3):182-4
references: 11231153
references: Development. 1991 May;112(1):1-20
references: 1685111
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Plant Cell. 2003 Apr;15(4):914-25
references: 12671087
references: Nature. 1991 Sep 5;353(6339):31-7
references: 1715520
references: Nature. 1999 Nov 25;402(6760):404-7
references: 10586879
references: Curr Biol. 2004 Nov 9;14(21):1935-40
references: 15530395
references: J Exp Bot. 2009;60(9):2517-27
references: 19443615
references: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references: 9396791
references: Curr Opin Plant Biol. 2001 Feb;4(1):75-85
references: 11163172
references: J Exp Zool B Mol Dev Evol. 2005 Nov 15;304(6):526-35
references: 15880769
references: Plant Cell. 1994 Sep;6(9):1211-25
references: 7919989
references: Planta. 2010 Jan;231(2):265-76
references: 19904556
references: Genes Dev. 1994 Jul 1;8(13):1548-60
references: 7958839
references: Plant J. 2010 Jun 1;62(5):807-16
references: 20230491
references: Mol Phylogenet Evol. 2003 Dec;29(3):464-89
references: 14615187