title: Sustained volume retention in vivo with adipocyte and lipoaspirate seeded silk scaffolds.
abstract: Current approaches to soft tissue regeneration include the use of fat grafts, natural or synthetic biomaterials as filler materials. Fat grafts and natural biomaterials resorb too quickly to maintain tissue regeneration, while synthetic materials do not degrade or regenerate tissue. Here, we present a simple approach to volume stable filling of soft tissue defects. In this study, we combined lipoaspirate with a silk protein matrix in a subcutaneous rat model. Silk biomaterials can be tailored to fit a variety of needs, and here were processed silk biomaterials into a porous sponge format to allow for tissue ingrowth while remaining mechanically robust. Over an 18 month period, the lipoaspirate seeded silk protein matrix regenerated subcutaneous adipose tissue while maintaining the original implanted volume. A silk protein matrix alone was not sufficient to regenerate adipose tissue, but yielded a fibrous tissue, although implanted volume was maintained. This work presents a significant improvement to the standard approaches to filling soft tissue defects by matching biomaterial degradation and tissue regeneration profiles.
authors: Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
authors: Evangelia
authors: E
authors: Bellas
authors: Bruce J B
authors: BJ
authors: Panilaitis
authors: Dean L
authors: DL
authors: Glettig
authors: Carl A
authors: CA
authors: Kirker-Head
authors: James J
authors: JJ
authors: Yoo
authors: Kacey G
authors: KG
authors: Marra
authors: J Peter
authors: JP
authors: Rubin
authors: David L
authors: DL
authors: Kaplan
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D047011:Silk; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D047011:Silk
references: Biomaterials. 2003 Feb;24(3):401-16
references: 12423595
references: J Cell Physiol. 2006 Jul;208(1):64-76
references: 16557516
references: J Cell Physiol. 2008 Feb;214(2):413-21
references: 17654479
references: J Drug Target. 2009 Apr;17(3):207-15
references: 19558360
references: Biomaterials. 2005 May;26(15):2775-85
references: 15585282
references: Plast Reconstr Surg (1946). 1951 Jan;7(1):6-23; contd
references: 14807738
references: J Cell Physiol. 2007 Sep;212(3):702-9
references: 17477371
references: Tissue Eng. 2006 Dec;12(12):3375-82
references: 17518674
references: Am J Physiol. 1984 Jan;246(1 Pt 1):C1-9
references: 6364825
references: Biomaterials. 2007 Dec;28(35):5280-90
references: 17765303
references: Methods Mol Biol. 2011;702:319-30
references: 21082412
references: Plast Reconstr Surg. 2002 Jul;110(1):123-9
references: 12087242
references: Nat Protoc. 2011 Sep 22;6(10):1612-31
references: 21959241
references: Plast Reconstr Surg. 2009 Mar;123(3):826-833
references: 19319045
references: Plast Reconstr Surg (1946). 1951 Feb;7(2):73-84
references: 14816047
references: Anat Rec. 2001 Aug 1;263(4):361-6
references: 11500812
references: Tissue Eng Part A. 2009 Aug;15(8):2227-36
references: 19207036
references: Aesthetic Plast Surg. 2001 Jan-Feb;25(1):57-63
references: 11322400
references: Tissue Eng Part B Rev. 2008 Jun;14(2):167-78
references: 18544014
references: Methods. 2009 Feb;47(2):116-21
references: 18952179
references: Biomaterials. 2008 Aug-Sep;29(24-25):3415-28
references: 18502501
references: Aesthetic Plast Surg. 2008 Jan;32(1):48-55; discussion 56-7
references: 17763894
references: Biomaterials. 2006 Dec;27(36):6052-63
references: 16973213
references: Prog Polym Sci. 2007;32(8-9):991-1007
references: 19543442
references: Biomaterials. 2003 Mar;24(6):893-900
references: 12504509
references: Biomaterials. 2004 Jun;25(14):2831-41
references: 14962561
references: Biomaterials. 2010 Apr;31(10):2926-33
references: 20044136