title: Polysaccharides from Bupleurum chinense impact the recruitment and migration of neutrophils by blocking fMLP chemoattractant receptor-mediated functions.
abstract: Although neutrophils play important roles in host defense, sometimes they contribute to inflammation-related tissue injuries. Thus, restriction of their recruitment to the inflammatory sites is a promising strategy in the amelioration of inflammatory disease. The previous studies reported the anti-inflammatory effects of Bupleurum chinense, but the active ingredients and possible mechanism are still unclear. Here, we isolated water-soluble polysaccharides (BCPs) from B. chinense, to evaluate its anti-inflammatory effects and possible mechanism. The present results showed that BCPs significantly impaired the in vivo neutrophil infiltration, as well as the migration capacity of dHL-60 cells in vitro. In addition, BCPs inhibits chemoattractant fMLP-induced activation and clustering of β2 integrin. BCPs impacted fMLP-induced actin polymerization and the activation of cytoskeleton regulatory molecules, Vav1 and Rac1. Together, BCPs significantly impacted recruitment and migration of neutrophils by blocking chemoattractant receptor-mediated functions, and it possesses a potential as novel anti-inflammatory drug.
authors: Life Science Research Center, Beihua University, Jilin 132013, China. tonghaibin@gmail.com
authors: Haibin
authors: H
authors: Tong
authors: Dan
authors: D
authors: Tian
authors: Zhongmei
authors: Z
authors: He
authors: Yang
authors: Y
authors: Liu
authors: Xiaodan
authors: X
authors: Chu
authors: Xin
authors: X
authors: Sun
mesh_terms: D000199:Actins / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D029972:Bupleurum / Q000737:chemistry*; D018821:CD18 Antigens / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism* / Q000494:pharmacology; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D011134:Polysaccharides / Q000494:pharmacology*; D055503:Protein Multimerization / Q000187:drug effects; D020836:Protein Structure, Quaternary; D051567:Proto-Oncogene Proteins c-vav / Q000378:metabolism; D044042:Receptors, Formyl Peptide / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: D000199:Actins; D000893:Anti-Inflammatory Agents; D018821:CD18 Antigens; D011134:Polysaccharides; D051567:Proto-Oncogene Proteins c-vav; D044042:Receptors, Formyl Peptide; C496618:VAV1 protein, human; D009240:N-Formylmethionine Leucyl-Phenylalanine; D020830:rac1 GTP-Binding Protein