title: Chromatin immunoprecipitation coupled by quantitative real-time PCR as a tool for analyzing epigenetic regulation of stem cell aging.
abstract: Epigenetic regulation is one of the major players in gene expression control. Histone modifications including acetylation or methylation are epigenetic mechanisms known to regulate senescence associated genes expression during adult stem cell aging. Chromatin immunoprecipitation (ChIP) assay is widely used to investigate histone modification patterns of genes of interest. For ChIP analysis, there are several conditions to be optimized empirically. In this article, not only a method of ChIP coupled by real-time quantitative PCR but also the detailed conditions is provided based on our previously published studies.
authors: Department of Veterinary Public Health, BK21 Program for Veterinary Science, Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. leewalwal@naver.com
authors: Seunghee
authors: S
authors: Lee
authors: Ji-Won
authors: JW
authors: Jung
authors: Kyung-Sun
authors: KS
authors: Kang
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D044127:Epigenesis, Genetic; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*