title: CD317/tetherin is an organiser of membrane microdomains.
abstract: The integral membrane protein tetherin has been associated with an eclectic mix of cellular processes, including restricting the release of a range of enveloped viruses from infected cells. The unusual topology of tetherin (it possesses both a conventional transmembrane domain and a glycosylphosphatidylinositol anchor), its localisation to membrane microdomains (lipid rafts) and the fact that its cytosolic domain can be linked (indirectly) to the actin cytoskeleton, led us to speculate that tetherin might form a 'tethered picket fence' and thereby play a role in the organisation of lipid rafts. We now show that knocking down expression of tetherin leads to changes in the distribution of lipid raft-localised proteins and changes in the organisation of lipids in the plasma membrane. These changes can be reversed by re-expression of wild-type tetherin, but not by any of a range of tetherin-based constructs, indicating that no individual feature of the tetherin sequence is dispensable in the context of its lipid raft organising function.
authors: School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK.
authors: Peter G
authors: PG
authors: Billcliff
authors: Ruth
authors: R
authors: Rollason
authors: Ian
authors: I
authors: Prior
authors: Dylan M
authors: DM
authors: Owen
authors: Katharina
authors: K
authors: Gaus
authors: George
authors: G
authors: Banting
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D058851:GPI-Linked Proteins / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D021962:Membrane Microdomains / Q000378:metabolism*; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D015703:Antigens, CD; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D016328:NF-kappa B; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2
keywords: Laurdan; Lipid raft; Membrane; Tetherin
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