title: Vitamin E-loaded silk fibroin nanofibrous mats fabricated by green process for skin care application.
abstract: In the present study, we reported fabrication and skin benefit of a novel vitamin E (VE)-loaded silk fibroin (SF) nanofibrous mats. RRR-α-Tocopherol polyethylene glycol 1000 succinate (VE TPGS), a water-soluble derivative of VE, was incorporated into SF nanofiber successfully by aqua solution electrospinning for the first time. Morphology of the composite nanofibers changed with the different amount of VE TPGS: a ribbon-like shape for lower loading dose of VE TPGS, while a round shape for higher loading dose (more than 4% (wt/wt) based on the weight of SF). After treated with 75% (v/v) ethanol vapor, the composite nanofibrous mats showed an excellent water-resistant ability. In vitro study disclosed a sustained release behavior of VE TPGS disassociated from the nanofibrous mats. The mouse skin fibroblasts (L929 cells) cultured on the VE-loaded SF nanofibrous mats spread and proliferated much better than on cover slips. Moreover, the incorporation of VE TPGS was found strengthening the ability of SF nanofibrous mats on protecting the cells against oxidation stress induced by tert-butyl hydroperoxide. Our data presented impressive skin benefits of this VE-loaded SF nanofibrous mats, suggesting a promising applicative potential of this novel product on personal skin care, tissue regeneration and other related area.
authors: Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors: Xiaoyue
authors: X
authors: Sheng
authors: Linpeng
authors: L
authors: Fan
authors: Chuanglong
authors: C
authors: He
authors: Kuihua
authors: K
authors: Zhang
authors: Xiumei
authors: X
authors: Mo
authors: Hongsheng
authors: H
authors: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology*; D055772:Green Chemistry Technology / Q000379:methods*; D051379:Mice; D008823:Micelles; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects; D011092:Polyethylene Glycols; D011108:Polymers / Q000737:chemistry; D017433:Protein Structure, Secondary; D017592:Skin Care / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013386:Succinates / Q000494:pharmacology; D014810:Vitamin E / Q000031:analogs & derivatives / Q000494:pharmacology*; D014961:X-Ray Diffraction; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D008823:Micelles; D011108:Polymers; D013386:Succinates; D014810:Vitamin E; D011092:Polyethylene Glycols; D005349:Fibroins; D020122:tert-Butylhydroperoxide; C014225:tocophersolan