title: Role of neural stem cell activity in postweaning development of the sexually dimorphic nucleus of the preoptic area in rats.
abstract: The sexually dimorphic nucleus of the preoptic area (SDN-POA) has received increased attention due to its apparent sensitivity to estrogen-like compounds found in food and food containers. The mechanisms that regulate SDN-POA volume remain unclear as is the extent of postweaning development of the SDN-POA. Here we demonstrate that the female Sprague-Dawley SDN-POA volume increased from weaning to adulthood, although this increase was not statistically significant as it was in males. The number of cells positive for Ki67, a marker of cell proliferation, in both the SDN-POA and the hypothalamus was significantly higher at weaning than at adulthood in male rats. In contrast, the number of Ki67-positive cells was significantly higher in the hypothalamus but not in the SDN-POA (p>0.05) at weaning than at adulthood in female rats. A subset of the Ki67-positive cells in the SDN-POA displayed the morphology of dividing cells. Nestin-immunoreactivity delineated a potential macroscopic neural stem cell niche in the rostral end of the 3rd ventricle. In conclusion, stem cells may partially account for the sexually dimorphic postweaning development of the SDN-POA.
authors: Division of Neurotoxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR, USA. zhen.he@fda.hhs.gov
authors: Zhen
authors: Z
authors: He
authors: Sherry A
authors: SA
authors: Ferguson
authors: Li
authors: L
authors: Cui
authors: L John
authors: LJ
authors: Greenfield
authors: Merle G
authors: MG
authors: Paule
mesh_terms: D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism*; D005260:Female; D021621:Imaging, Three-Dimensional; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D011301:Preoptic Area / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics; D055153:Stem Cell Niche; D014886:Weaning
chemical_list: D019394:Ki-67 Antigen
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