title: Optogenetic protein clustering and signaling activation in mammalian cells.
abstract: We report an optogenetic method based on Arabidopsis thaliana cryptochrome 2 for rapid and reversible protein oligomerization in response to blue light. We demonstrated its utility by photoactivating the β-catenin pathway, achieving a transcriptional response higher than that obtained with the natural ligand Wnt3a. We also demonstrated the modularity of this approach by photoactivating RhoA with high spatiotemporal resolution, thereby suggesting a previously unknown mode of activation for this Rho GTPase.
authors: Department of Bioengineering, University of California, Berkeley (UC Berkeley), Berkeley, California, USA.
authors: Lukasz J
authors: LJ
authors: Bugaj
authors: Atri T
authors: AT
authors: Choksi
authors: Colin K
authors: CK
authors: Mesuda
authors: Ravi S
authors: RS
authors: Kane
authors: David V
authors: DV
authors: Schaffer
mesh_terms: D000818:Animals; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D056931:Cryptochromes / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D003593:Cytoplasm / Q000378:metabolism / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D036681:Fluorescence Recovery After Photobleaching; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D055537:Light Signal Transduction; D008164:Luminescent Proteins / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D041681:NIH 3T3 Cells; D055503:Protein Multimerization / Q000528:radiation effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000528:radiation effects; D014158:Transcription, Genetic; D014162:Transfection; D060449:Wnt Signaling Pathway / Q000528:radiation effects; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D000097573:Red Fluorescent Protein
chemical_list: D029681:Arabidopsis Proteins; D056931:Cryptochromes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D060509:Wnt3A Protein; D051176:beta Catenin; D020742:rhoA GTP-Binding Protein; D000097573:Red Fluorescent Protein; C103618:CRY2 protein, Arabidopsis
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