title: Cold stress causes rapid but differential changes in properties of plasma membrane H(+)-ATPase of camelina and rapeseed.
abstract: Camelina (Camelina sativa) and rapeseed (Brassica napus) are well-established oil-seed crops with great promise also for biofuels. Both are cold-tolerant, and camelina is regarded to be especially appropriate for production on marginal lands. We examined physiological and biochemical alterations in both species during cold stress treatment for 3 days and subsequent recovery at the temperature of 25°C for 0, 0.25, 0.5, 1, 2, 6, and 24h, with particular emphasis on the post-translational regulation of the plasma membrane (PM) H(+)-ATPase (EC3.6.3.14). The activity and translation of the PM H(+)-ATPase, as well as 14-3-3 proteins, increased after 3 days of cold stress in both species but recovery under normal conditions proceeded differently. The increase in H(+)-ATPase activity was the most dramatic in camelina roots after recovery for 2h at 25°C, followed by decay to background levels within 24h. In rapeseed, the change in H(+)-ATPase activity during the recovery period was less pronounced. Furthermore, H(+)-pumping increased in both species after 15min recovery, but to twice the level in camelina roots compared to rapeseed. Protein gel blot analysis with phospho-threonine anti-bodies showed that an increase in phosphorylation levels paralleled the increase in H(+)-transport rate. Thus our results suggest that cold stress and recovery in camelina and rapeseed are associated with PM H(+)-fluxes that may be regulated by specific translational and post-translational modifications.
authors: Department of Bioenergy Science and Technology, Bio-energy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Hyun-Sung
authors: HS
authors: Kim
authors: Jung-Min
authors: JM
authors: Oh
authors: Sheng
authors: S
authors: Luan
authors: John E
authors: JE
authors: Carlson
authors: Sung-Ju
authors: SJ
authors: Ahn
mesh_terms: D001692:Biological Transport; D029741:Brassica rapa / Q000201:enzymology* / Q000502:physiology; D019607:Brassicaceae / Q000201:enzymology* / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology; D002734:Chlorophyll / Q000378:metabolism; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D010766:Phosphorylation; D018515:Plant Leaves / Q000201:enzymology / Q000502:physiology; D018517:Plant Roots / Q000201:enzymology / Q000502:physiology; D054046:Plant Stomata / Q000201:enzymology / Q000502:physiology; D018526:Plant Transpiration / Q000502:physiology; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D036226:Seedlings / Q000201:enzymology / Q000502:physiology; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D002734:Chlorophyll; D006180:Proton-Translocating ATPases