title: Proteomics in epigenetics: new perspectives for cancer research.
abstract: The involvement of epigenetic processes in the origin and progression of cancer is now widely appreciated. Consequently, targeting the enzymatic machinery that controls the epigenetic regulation of the genome has emerged as an attractive new strategy for therapeutic intervention. The development of epigenetic drugs requires a detailed knowledge of the processes that govern chromatin regulation. Over the recent years, mass spectrometry (MS) has become an indispensable tool in epigenetics research. In this review, we will give an overview of the applications of MS-based proteomics in studying various aspects of chromatin biology. We will focus on the use of MS in the discovery and mapping of histone modifications and how novel proteomic approaches are being utilized to identify and study chromatin-associated proteins and multi-subunit complexes. Finally, we will discuss the application of proteomic methods in the diagnosis and prognosis of cancer based on epigenetic biomarkers and comment on their future impact on cancer epigenetics.
authors: MRC Clinical Sciences Centre, Imperial College London Faculty of Medicine, Hammersmith Hospital Campus, London W12 0NN, UK. till.bartke@csc.mrc.ac.uk
authors: Till
authors: T
authors: Bartke
authors: Julie
authors: J
authors: Borgel
authors: Peter A
authors: PA
authors: DiMaggio
mesh_terms: D000818:Animals; D057890:Epigenomics / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
keywords: Mass spectrometry; cancer epigenetics; chromatin; epigenetic readers; histone modifications; quantitative proteomics
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