title: Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles.
abstract: Recombinant hepatitis B virus core proteins dimerize to form building blocks that are capable of self-assembly into a capsid. A core capsid protein dimer (CPD) linked to a green fluorescent protein variant, EGFP, at the C-terminus has been designed. The recombinant fusion CPD was expressed in Escherichia coli, assembled into virus-like particles (VLPs), purified and crystallized. The single crystal diffracted to 2.15 Å resolution and belonged to the cubic space group F432, with unit-cell parameters a = b = c = 219.7 Å. The fusion proteins assembled into icosahedral VLPs in aqueous solution, but were rearranged into octahedral symmetry through the crystal-packing process under the crystallization conditions.
authors: Faculty of Pharmaceutical Sciences, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba 288-0025, Japan.
authors: Masaki
authors: M
authors: Kikuchi
authors: Shinichiro
authors: S
authors: Iwabuchi
authors: Tatsuhiko
authors: T
authors: Kikkou
authors: Keiichi
authors: K
authors: Noguchi
authors: Masafumi
authors: M
authors: Odaka
authors: Masafumi
authors: M
authors: Yohda
authors: Masaaki
authors: M
authors: Kawata
authors: Chikara
authors: C
authors: Sato
authors: Osamu
authors: O
authors: Matsumoto
mesh_terms: D002213:Capsid / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006515:Hepatitis B virus / Q000378:metabolism* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D055503:Protein Multimerization; D014461:Ultracentrifugation; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D014771:Virion / Q000648:ultrastructure
chemical_list: D014760:Viral Fusion Proteins
keywords: Escherichia coli; fusion protein; green fluorescent protein; hepatitis B virus; octahedral symmetry; virus-like particle
references: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
references: 16369096
references: Nat Struct Biol. 1997 Apr;4(4):269-75
references: 9095194
references: Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):271-81
references: 21460445
references: Science. 1981 Jul 24;213(4506):406-11
references: 6264599
references: J Mol Biol. 1968 Apr 28;33(2):491-7
references: 5700707
references: Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9556-61
references: 9275161
references: Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
references: 20057045
references: Nucleic Acids Res. 1988 Aug 11;16(15):7351-67
references: 3045756
references: Nucleic Acids Res. 1996 Jun 15;24(12):2352-9
references: 8710507
references: FEBS Lett. 2000 Sep 15;481(2):169-76
references: 10996318
references: Science. 2006 May 12;312(5775):873-5
references: 16690856
references: Nat Biotechnol. 1996 Oct;14(10):1246-51
references: 9631087
references: J Mol Biol. 2006 Feb 24;356(3):812-22
references: 16378623
references: Mol Cell. 1999 Jun;3(6):771-80
references: 10394365
references: Cell. 1994 Jun 17;77(6):943-50
references: 8004680
references: J Biol Chem. 2006 Jun 23;281(25):17474-17481
references: 16621801
references: Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):717-20
references: 10089479
references: J Gen Virol. 1997 Aug;78 ( Pt 8):2049-53
references: 9267006
references: Protein Sci. 2008 Oct;17(10):1731-9
references: 18662904
references: FEBS Lett. 2005 Sep 26;579(23):5211-6
references: 16162343
references: J Mol Biol. 1986 Aug 20;190(4):587-91
references: 3783713
references: ACS Nano. 2011 Nov 22;5(11):8690-9
references: 21985460