title: Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal.
abstract: Carica papaya (papaya) is a fruit crop that is cultivated mostly in kitchen gardens throughout Nepal. Leaf samples of C. papaya plants with leaf curling, vein darkening, vein thickening, and a reduction in leaf size were collected from a garden in Darai village, Rampur, Nepal in 2010. Full-length clones of a monopartite Begomovirus, a betasatellite and an alphasatellite were isolated. The complete nucleotide sequence of the Begomovirus showed the arrangement of genes typical of Old World begomoviruses with the highest nucleotide sequence identity (>99 %) to an isolate of Ageratum yellow vein virus (AYVV), confirming it as an isolate of AYVV. The complete nucleotide sequence of betasatellite showed greater than 89 % nucleotide sequence identity to an isolate of Tomato leaf curl Java betasatellite originating from Indonesian. The sequence of the alphasatellite displayed 92 % nucleotide sequence identity to Sida yellow vein China alphasatellite. This is the first identification of these components in Nepal and the first time they have been identified in papaya.
authors: Department of International Agricultural Development, Tokyo University of Agriculture, Tokyo, 156-8502, Japan. shafiqinayat@gmail.com
authors: M S
authors: MS
authors: Shahid
authors: S
authors: S
authors: Yoshida
authors: G B
authors: GB
authors: Khatri-Chhetri
authors: R W
authors: RW
authors: Briddon
authors: K T
authors: KT
authors: Natsuaki
mesh_terms: D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification; D029441:Carica / Q000821:virology*; D016000:Cluster Analysis; D004276:DNA, Satellite / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009390:Nepal; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004276:DNA, Satellite; D004279:DNA, Viral
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