title: Expression profiles of microRNAs and their target genes in papillary             thyroid carcinoma.
abstract: The incidence of thyroid cancer has recently experienced a rapid increase             in China, and papillary thyroid carcinoma (PTC) accounts for nearly 80% of human             thyroid cancers. In the present study, the differential expression of microRNAs             (miRNAs) and their target genes were identified in order to analyze the potential             roles of miRNAs as biomarkers and in papillary thyroid carcinogenesis. One hundred             and twenty-six PTC samples were collected from patients at the China-Japan Union             Hospital, China, and the gene/miRNA expression profiles were examined with Illumina             BeadChips and verified by real‑time RT-PCR. Gene Ontology (GO) categories were             determined, and pathway analysis was carried out using KEGG. miRNA target genes             were predicted by implementing three computational analysis programs: TargetScanS,             DIANA-microT and PicTar. Two hundred and forty-eight miRNAs and 3,631 genes were             found to be significantly deregulated (gene, P<0.05; miRNA, P<0.01) in PTC             tissues when compared with their matching normal thyroid tissues. hsa-miR-206             (target gene, MET), hsa-miR-299-3p (target gene, ITGAV), hsa-miR-101 (target gene,             ITGA3), hsa-miR-103 (target gene, ITGA2), hsa-miR‑222 (target genes, KIT and AXIN2),             hsa-miR-15a (target genes, AXIN2 and FOXO1) and hsa-mir-221 (target gene, KIT)             were identified. Together with the functions of the target genes, we further elucidated             the role of miRNAs in papillary thyroid carcinogenesis and suggest the use of             miRNAs as biomarkers for early diagnosis. Our findings provide the basis for future             studies in the field of miRNA-based cancer therapy.
authors: Department of Bioinformatics and Statistics, School of Public Health, Jilin University, Changchun 130021, PR China.
authors: Xin
authors: X
authors: Liu
authors: Mengzi
authors: M
authors: He
authors: Yufei
authors: Y
authors: Hou
authors: Bing
authors: B
authors: Liang
authors: Longyu
authors: L
authors: Zhao
authors: Shumei
authors: S
authors: Ma
authors: Yaqin
authors: Y
authors: Yu
authors: Xiaodong
authors: X
authors: Liu
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D058990:Molecular Targeted Therapy; D020411:Oligonucleotide Array Sequence Analysis; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D035683:MicroRNAs