title: Liposomal surface coatings of metal stents for efficient non-viral gene delivery to the injured vasculature.
abstract: Despite the widespread use of drug eluting stents (DES), in-stent restenosis (ISR), delayed arterial healing and thrombosis remain important clinical complications. Gene-eluting stents (GES) represent a potential strategy for the prevention of ISR by delivering a therapeutic gene via a vector from the stent surface to the vessel wall. To this end, a model in vitro system was established to examine whether cationic liposomes could be used for gene delivery to human artery cells. Three different formulations were compared (DOTMA/DOPE, DDAB/DOPE or DDAB/POPC/Chol) to examine the effects of different cationic and neutral lipids on the transfection efficiency of lipoplex-coatings of metal surfaces. Upon completion of the characterization and optimization of the materials for gene delivery in vitro, these coatings were examined on a range of stents and deployed in a rabbit iliac artery injury model in vivo. Maximal transfection efficiencies for all coatings were observed on day 28, followed by declining, but persisting gene expression 42 days after stent placement, thereby, presenting liposomal coatings for gene eluting stents as treatment options for clinical complications associated with stenting procedures.
authors: Regenerative Medicine Institute, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.
authors: Sandra
authors: S
authors: Ganly
authors: Sean O
authors: SO
authors: Hynes
authors: Faisal
authors: F
authors: Sharif
authors: Ahmed
authors: A
authors: Aied
authors: Valerie
authors: V
authors: Barron
authors: Karl
authors: K
authors: McCullagh
authors: Jill
authors: J
authors: McMahon
authors: Peter
authors: P
authors: McHugh
authors: Jim
authors: J
authors: Crowley
authors: Wenxin
authors: W
authors: Wang
authors: Timothy
authors: T
authors: O'Brien
authors: Udo
authors: U
authors: Greiser
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D002858:Chromium Alloys; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007083:Iliac Artery / Q000293:injuries / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D008297:Male; D032389:Myocytes, Smooth Muscle; D010957:Plasmids; D011817:Rabbits; D013193:Stainless Steel; D015607:Stents; D013499:Surface Properties; D014162:Transfection / Q000379:methods*; D014709:Vero Cells; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D002858:Chromium Alloys; D008055:Lipids; D008081:Liposomes; D013193:Stainless Steel; D049452:Green Fluorescent Proteins; D004247:DNA; D001616:beta-Galactosidase