title: Sustained therapeutic perfusion outside transplanted sites in chronic myocardial infarction after stem cell transplantation.
abstract: This study aimed at comparing long-term variations in the perfusion of chronic myocardial infarction (MI) areas after local injections of autologous bone marrow stem cells (BMSCs). 14 coronary ligated rats with transmural chronic MI (4 months) were used: a control group (n = 7) versus a treated group (n = 7) in which (111)In labeled-BMSCs were directly engrafted on MI areas. By using (111)In/(99m)Tc SPECT and Sestamibi gated-SPECT,. left ventricle perfusion and function were monitored in all animals by serial (99m)Tc-Sestamibi pinhole gated-SPECT over a period of 6 months. Post-therapeutic myocardial perfusion improved as early as 48 h following injection in the 2 groups. This benefice was sustained during the 6-month follow-up in the non-engrafted MI-areas from treated rats (at 6-months: +10 ± 5 %), whereas the engrafted ones, as well as the MI areas from control rats, exhibited progressive deterioration over time (at 6-months: -9 ± 10 % and -5 ± 3 %, respectively). Perfusion enhancement of the chronic MI areas treated by BMSCs transplantation is: (1) marked in the following days, presumably because of an unspecific inflammatory reaction, and (2) sustained over the long term but only outside the sites of cell engraftment, suggesting a distant paracrine effect of transplanted cells.
authors: Faculty of Medicine, School of Surgery, University of Lorraine, 9 Avenue de Forêt de Haye, 54500 Vandoeuvre-lès-Nancy, France. pablomaureira@hotmail.com
authors: Pablo
authors: P
authors: Maureira
authors: Pierre-Yves
authors: PY
authors: Marie
authors: Yihua
authors: Y
authors: Liu
authors: Fengxu
authors: F
authors: Yu
authors: Sylvain
authors: S
authors: Poussier
authors: Fatiha
authors: F
authors: Maskali
authors: Frederique
authors: F
authors: Groubatch
authors: Gilles
authors: G
authors: Karcher
authors: Nguyen
authors: N
authors: Tran
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003326:Coronary Circulation; D004195:Disease Models, Animal; D007205:Indium Radioisotopes; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D055414:Myocardial Perfusion Imaging / Q000379:methods; D009206:Myocardium / Q000473:pathology*; D019275:Radiopharmaceuticals; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D012038:Regeneration; D033581:Stem Cell Transplantation; D017256:Technetium Tc 99m Sestamibi; D013997:Time Factors; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D007205:Indium Radioisotopes; D019275:Radiopharmaceuticals; D017256:Technetium Tc 99m Sestamibi
references: Cell Transplant. 2006;15(4):351-8
references: 16898229
references: J Mol Cell Cardiol. 2002 Jul;34(7):765-74
references: 12099716
references: J Nucl Med. 2006 Feb;47(2):337-44
references: 16455641
references: Eur J Nucl Med Mol Imaging. 2006 Jun;33(6):709-15
references: 16572303
references: Can J Cardiol. 2007 Sep;23(11):891-8
references: 17876381
references: Regen Med. 2007 Jan;2(1):63-8
references: 17465776
references: Stem Cells. 2007 Aug;25(8):2118-27
references: 17478584
references: Am J Physiol Heart Circ Physiol. 2003 Dec;285(6):H2420-9
references: 12881220
references: Am J Physiol Heart Circ Physiol. 2009 Apr;296(4):H927-36
references: 19181964
references: Regul Pept. 2004 Jan 15;117(1):3-10
references: 14687695
references: Circulation. 2004 Sep 14;110(11 Suppl 1):II219-24
references: 15364866
references: Eur J Nucl Med Mol Imaging. 2002 Apr;29(4):465-72
references: 11914883
references: Eur J Heart Fail. 2009 Mar;11(3):238-45
references: 19147447
references: J Nucl Med. 2007 Mar;48(3):405-12
references: 17332618
references: J Nucl Med. 2005 Nov;46(11):1789-95
references: 16269591
references: Circulation. 2005 Jul 12;112(2):214-23
references: 15998673
references: Clin Nucl Med. 2012 Aug;37(8):738-42
references: 22785499
references: Am J Physiol Heart Circ Physiol. 2007 Sep;293(3):H1772-80
references: 17573463
references: FASEB J. 2007 Oct;21(12):3197-207
references: 17496162
references: Am J Cardiol. 2007 Oct 1;100(7):1094-8
references: 17884369
references: Eur J Nucl Med Mol Imaging. 2007 Feb;34(2):170-80
references: 16953400
references: Tissue Eng Part A. 2009 Sep;15(9):2459-70
references: 19327020
references: Circ Res. 2008 Nov 21;103(11):1300-8
references: 18948617
references: Mod Pathol. 2008 Jul;21(7):837-46
references: 18487998
references: Ann Thorac Surg. 2005 Jul;80(1):229-36; discussion 236-7
references: 15975372
references: Am J Physiol Heart Circ Physiol. 2008 Dec;295(6):H2308-14
references: 18849336
references: Cell Transplant. 2007;16(9):879-86
references: 18293886
references: Eur Heart J. 2008 Jan;29(2):251-9
references: 18073226
references: Eur J Cardiothorac Surg. 2007 Mar;31(3):438-43
references: 17239611
references: Mol Biol Rep. 2009 Apr;36(4):725-31
references: 18368514
references: Circulation. 2007 Apr 10;115(14):1866-75
references: 17389266
references: Circulation. 2005 Aug 30;112(9 Suppl):I96-104
references: 16159872
references: J Cell Biochem. 2006 Aug 1;98(5):1076-84
references: 16619257
references: Transpl Int. 2008 Dec;21(12):1181-9
references: 18783386
references: J Am Coll Cardiol. 1999 Nov 15;34(6):1663-70
references: 10577553