title: Use of array comparative genomic hybridization (array-CGH) for embryo assessment: clinical results.
abstract: OBJECTIVE
  To review clinical outcomes after preimplantation genetic screening. Most methods of embryo viability assessment involve morphologic evaluation at different preimplantation developmental stages. A weak association between blastocyst morphology and aneuploidy has been described, supporting the basis for preimplantation genetic screening (PGS) for assessment of embryo viability. The expected improvement in reproductive outcome rates has been reached with the application of microarrays based on comparative genomic hybridization (CGH) in clinical routine PGS.
  
  
  METHODS
  Review of published studies and own unpublished data.
  
  
  METHODS
  University-affiliated private institution.
  
  
  METHODS
  IVF patients undergoing PGS at different stages.
  
  
  METHODS
  PGS with polar body, cleavage-stage, and blastocyst biopsies.
  
  
  METHODS
  Aneuploidy, implantation, and pregnancy rates.
  
  
  RESULTS
  The clinical outcome after analysis of all 24 chromosomes improved pregnancy and implantation rates for different indications to a higher degree than the previously available technology, fluorescence in situ hybridization (FISH), in which only a limited number of chromosomes could be analyzed.
  
  
  CONCLUSIONS
  Most of the data regarding the controversy of day-3 biopsy come from FISH cycles, and the utility of day-3 biopsy with new array-CGH technology should be further evaluated through randomized controlled trials. The current trend is blastocyst biopsy with a fresh transfer or vitrification for transfer in a nonstimulated cycle.
authors: Instituto Valenciano de Infertilidad, Instituto Universitario IVI, Valencia University, Incliva, Valencia, Spain. carmen.rubio@iviomics.com
authors: Carmen
authors: C
authors: Rubio
authors: Lorena
authors: L
authors: Rodrigo
authors: Pere
authors: P
authors: Mir
authors: Emilia
authors: E
authors: Mateu
authors: Vanessa
authors: V
authors: Peinado
authors: Miguel
authors: M
authors: Milán
authors: Nasser
authors: N
authors: Al-Asmar
authors: Inmaculada
authors: I
authors: Campos-Galindo
authors: Sandra
authors: S
authors: Garcia
authors: Carlos
authors: C
authors: Simón
mesh_terms: D001755:Blastocyst / Q000502:physiology*; D055028:Comparative Genomic Hybridization / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*