title: Cytological characterization and allelism testing of anther developmental mutants identified in a screen of maize male sterile lines.
abstract: Proper regulation of anther differentiation is crucial for producing functional pollen, and defects in or absence of any anther cell type result in male sterility. To deepen understanding of processes required to establish premeiotic cell fate and differentiation of somatic support cell layers a cytological screen of maize male-sterile mutants has been conducted which yielded 42 new mutants including 22 mutants with premeiotic cytological defects (increasing this class fivefold), 7 mutants with postmeiotic defects, and 13 mutants with irregular meiosis. Allelism tests with known and new mutants confirmed new alleles of four premeiotic developmental mutants, including two novel alleles of msca1 and single new alleles of ms32, ms8, and ocl4, and two alleles of the postmeiotic ms45. An allelic pair of newly described mutants was found. Premeiotic mutants are now classified into four categories: anther identity defects, abnormal anther structure, locular wall defects and premature degradation of cell layers, and/or microsporocyte collapse. The range of mutant phenotypic classes is discussed in comparison with developmental genetic investigation of anther development in rice and Arabidopsis to highlight similarities and differences between grasses and eudicots and within the grasses.
authors: Department of Molecular and Cell Biology, University of California at Berkeley, California 94720, USA.
authors: Ljudmilla
authors: L
authors: Timofejeva
authors: David S
authors: DS
authors: Skibbe
authors: Sidae
authors: S
authors: Lee
authors: Inna
authors: I
authors: Golubovskaya
authors: Rachel
authors: R
authors: Wang
authors: Lisa
authors: L
authors: Harper
authors: Virginia
authors: V
authors: Walbot
authors: William Zacheus
authors: WZ
authors: Cande
mesh_terms: D000483:Alleles; D049109:Cell Proliferation; D017343:Genes, Plant; D008540:Meiosis; D009154:Mutation; D010940:Plant Proteins / Q000145:classification / Q000235:genetics; D011058:Pollen / Q000235:genetics / Q000254:growth & development; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development
chemical_list: D010940:Plant Proteins
keywords: anther development; cell fate acquisition; maize; male sterility
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