title: Rapid separation and sensitive determination of banned aromatic amines with plastic microchip electrophoresis.
abstract: Rapid analysis of trace amount of aromatic amines in environmental samples and daily necessities has attracted considerable attentions because some of them are strongly toxic and carcinogenic. In this study, fast and efficient electrophoretic separation and sensitive determination of 5 banned aromatic amines were explored for practical analysis using disposable plastic microchips combined with a low-cost laser-induced fluorescence detector. The effect of running buffer and its additive was systematically investigated. Under the selected condition, 5 fluorescein isothiocyanate labeled aromatic amines could be baseline separated within 90s by using a 10mmol/L borate buffer containing 2% (w/v) hydroxypropyl cellulose. Calibration curves of peak areas vs. concentrations were linear up to 40 or 120μmol/L for different analytes and limits of detection were in a range of 1-3nmol/L. Theoretical plate numbers of 6.8-8.5×10(5)/m were readily achieved. The method exhibited good repeatability, relative standard deviations (n=5) of peak areas and migration times were no more than 4.6% and 0.9%, respectively. The established method was successfully applied in the quantitative analysis of these banned aromatic amines in real samples of waste water and textile, recoveries of added standards were 85-110%.
authors: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors: Ruina
authors: R
authors: Li
authors: Lili
authors: L
authors: Wang
authors: Xiaotong
authors: X
authors: Gao
authors: Gangfeng
authors: G
authors: Du
authors: Honglin
authors: H
authors: Zhai
authors: Xiayan
authors: X
authors: Wang
authors: Guangsheng
authors: G
authors: Guo
authors: Qiaosheng
authors: Q
authors: Pu
mesh_terms: D000588:Amines / Q000032:analysis*; D001881:Borates / Q000737:chemistry; D046209:Electrophoresis, Microchip; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D010969:Plastics; D045483:Rivers / Q000737:chemistry*; D013784:Textiles / Q000032:analysis*; D062065:Wastewater / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000588:Amines; D001881:Borates; D007220:Industrial Waste; D010969:Plastics; D062065:Wastewater; D014874:Water Pollutants, Chemical; C010634:sodium borate