title: [Effects of BDNF antibody block on IL-6 expression in lung of rats subjected to brain ischemia].
abstract: OBJECTIVE
  To explore effect of brain-derived neurotrophic factor (BDNF) on IL-6 expression in the lung of rats with brain ischemia.
  
  
  METHODS
  Inflammatory lung injury was induced by brain ischemia in rats that were devided into sham operation group, brain ischemia lung injury (BILI) group and brain ischemia with BDNF administration group. Lungs were harvested from rats in each group in 3 days after brain ischemia respectively. Immunohistochemistry, RT-PCR and Western blot were employed to detect the expressional changes on the mRNA and protein of IL-6 in the lung tissues.
  
  
  RESULTS
  Expression of IL-6 was mainly found in cytoplasm of lung epithelial cell and macrophage with immunohistochemistry staining. A significant decrease of IL-6 expression was observed at 3 days after brain ischemia with BDNF antibody block.
  
  
  CONCLUSIONS
  BDNF, as a crucial neurotrophic factor, could regulate IL-6 expression in rats lung tissues with brain ischemia.
authors: Department of Respiration, Affiliated Kunhua Hospital of Kunming Science and Technology University, Kunming 650032, China.
authors: Yun
authors: Y
authors: Yang
authors: Rong
authors: R
authors: Rong
authors: Qi-Qin
authors: QQ
authors: Dan
authors: Run-Fen
authors: RF
authors: Du
authors: Jin-Tao
authors: JT
authors: Li
authors: Yun-Hui
authors: YH
authors: Zhang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D055370:Lung Injury / Q000209:etiology / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000906:Antibodies; D019208:Brain-Derived Neurotrophic Factor; D015850:Interleukin-6; D012333:RNA, Messenger