title: Adiponectin modulates NK-cell function.
abstract: Adiponectin (APN) has been shown to exert antiinflammatory effects in various disease models but little is known concerning its regulation of NK-cell function. Here, we show that the majority of human CD56(dim) NK cells express surface Adiponectin receptor (AdipoR) 1 and 2 while most CD56(high) NK cells are AdipoR-negative. Toll-like receptor (TLR) ligand-induced IFN-γ production was diminished by APN while it had no influence on NK-cell cytotoxicity. In contrast only a small subpopulation of murine NK cells expresses surface AdipoRs, but about 90% store them intracellularly. APN-deficient knockout (KO) mice had elevated frequencies of NK cells. However, cytotoxic degranulation of NK cells was decreased in APN knockout (APN-KO) animals. Accordingly, frequencies of CD11b(high) CD27(high) and CD94(high) effector NK cells and expression of NKG2D were lower in APN-KO mice. Upon CVB3 infection NK-cell function was restored in APN-KO mice. Our data suggest that in addition to its antiinflammatory effects APN also influences the numerical and differentiation status of NK cells, which may further impact the outcome of immune-mediated diseases in APN-KO mice.
authors: Institute of Medical Immunology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors: Sabrina
authors: S
authors: Wilk
authors: Alexander
authors: A
authors: Jenke
authors: Jenny
authors: J
authors: Stehr
authors: Chin-An
authors: CA
authors: Yang
authors: Sandra
authors: S
authors: Bauer
authors: Katrin
authors: K
authors: Göldner
authors: Katja
authors: K
authors: Kotsch
authors: Hans-Dieter
authors: HD
authors: Volk
authors: Wolfgang
authors: W
authors: Poller
authors: Heinz-Peter
authors: HP
authors: Schultheiss
authors: Carsten
authors: C
authors: Skurk
authors: Carmen
authors: C
authors: Scheibenbogen
mesh_terms: D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015550:Cell Degranulation / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D018345:Mice, Knockout; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D052242:Adiponectin; D008024:Ligands; D054419:Receptors, Adiponectin; D051193:Toll-Like Receptors; D007371:Interferon-gamma