title: Virus-induced gene silencing in the rapid cycling columbine Aquilegia coerulea "Origami".
abstract: Aquilegia Origami is an emerging model system for ecology and evolution, which has numerous genetic and genomic tools. Virus-induced gene silencing (VIGS) has been established as an effective approach to study gene function in Aquilegia. In the current protocol, we demonstrate VIGS using Agrobacterium strain GV3101 carrying tobacco rattle virus (TRV)-based constructs to infect Aquilegia coerulea "Origami" plants via vacuum infiltration.
authors: Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
authors: Bharti
authors: B
authors: Sharma
authors: Elena M
authors: EM
authors: Kramer
mesh_terms: D016960:Agrobacterium tumefaciens / Q000821:virology; D031884:Aquilegia / Q000235:genetics* / Q000821:virology; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D017343:Genes, Plant; D005822:Genetic Vectors; D010641:Phenotype; D010942:Plant Viruses / Q000235:genetics*; D034622:RNA Interference; D014169:Transformation, Bacterial