title: Effects of hyperbaric oxygen (HBO), as pre-conditioning in liver of rats submitted to periodic liver ischemia/reperfusion.
abstract: OBJECTIVE
  to assess the effect of hyperbaric oxygen (HBO) as pre-conditioning on periodic liver ischemia/reperfusion injury.
  
  
  METHODS
  Thirty-six male Wistar rats were divided into 4 groups (SHAM, I/R , HBO-I/R and CONTROL). The surgical technique consisted of total clamping of the hepatic pedicle for 15 min followed by twice repeated reperfusion for 5 min (unclamping). HBO was applied in a collective chamber (simultaneous exposure of 4 rats) directly pressurized with oxygen at 2 ATA for 60 min. Hepatic mitochondrial function was determined using samples of the median lobe obtained after exactly 5 min of reperfusion for the analysis of mitochondrial respiration based on the determination of states 3 and 4, the respiratory control ratio and the transition of mitochondrial permeability (mitochondrial swelling).Data were analyzed by the Mann-Whitney test and the level of significance was set at p < 0.05.
  
  
  RESULTS
  There was a statistically significant difference (p < 0.05) in state 3 between the CONTROL and I/R and HBO-I/R groups, in state 4 between the CONTROL and I/R and HBO-I/R groups; in respiratory control ratio (RCR) between the CONTROL and I/R and HBO-I/R groups and between the CONTROL and Sham groups, and in mitochondrial swelling between the CONTROL and I/R and HBO-/R groups and between the Sham and I/R and HBO-I/R groups.
  
  
  CONCLUSIONS
  In this process of periodic ischemia and reperfusion, hyperbaric pre-conditioning did not improve significantly hepatic mitochondrial function.
authors: Marilia Faculty of Medicine, Marilia, SP, Brazil.
authors: Diego Elias da Silva
authors: DE
authors: Caldeira
authors: Maria Eliza Jordani
authors: ME
authors: Souza
authors: Maria Cecília Jordani
authors: MC
authors: Gomes
authors: Maria Aparecida Neves Cardoso
authors: MA
authors: Picinato
authors: Clarice Fleury
authors: CF
authors: Fina
authors: Omar
authors: O
authors: Feres
authors: Orlando
authors: O
authors: Castro e Silva
mesh_terms: D000818:Animals; D019069:Cell Respiration; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D019194:Ischemic Preconditioning / Q000379:methods*; D008099:Liver / Q000503:physiopathology; D008297:Male; D008930:Mitochondria, Liver / Q000502:physiology*; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D015427:Reperfusion Injury / Q000503:physiopathology / Q000628:therapy*