title: Development of bacteriostatic DNA aptamers for salmonella.
abstract: Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Aptamers have grown in popularity since their discovery, and their properties make them attractive candidates for therapeutic use. In this work, we describe the selection of highly specific DNA aptamers to S. enteritidis and S. typhimurium. To evolve species-specific aptamers, twelve rounds of selection to live S. enteritidis and S. typhimurium were performed, alternating with a negative selection against a mixture of related pathogens. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential.
authors: Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka str., Krasnoyarsk 660022, Russia.
authors: Olga S
authors: OS
authors: Kolovskaya
authors: Anna G
authors: AG
authors: Savitskaya
authors: Tatiana N
authors: TN
authors: Zamay
authors: Irina T
authors: IT
authors: Reshetneva
authors: Galina S
authors: GS
authors: Zamay
authors: Evgeny N
authors: EN
authors: Erkaev
authors: Xiaoyan
authors: X
authors: Wang
authors: Mohamed
authors: M
authors: Wehbe
authors: Alla B
authors: AB
authors: Salmina
authors: Olga V
authors: OV
authors: Perianova
authors: Olga A
authors: OA
authors: Zubkova
authors: Ekaterina A
authors: EA
authors: Spivak
authors: Vasily S
authors: VS
authors: Mezko
authors: Yury E
authors: YE
authors: Glazyrin
authors: Nadezhda M
authors: NM
authors: Titova
authors: Maxim V
authors: MV
authors: Berezovski
authors: Anna S
authors: AS
authors: Zamay
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial; D008969:Molecular Sequence Data; D052156:SELEX Aptamer Technique; D012477:Salmonella enteritidis / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide