title: Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO.
abstract: The gene coding for ribose-5-phosphate isomerase (Rpi) from Thermotoga lettingae TMO was cloned and expressed in E. coli. The recombinant enzyme was purified by Ni-affinity chromatography. It converted D-psicose to D-allose maximally at 75 °C and pH 8.0 with a 32 % conversion yield. The k m, turnover number (k cat), and catalytic efficiency (k cat k m (-1) ) for substrate D-psicose were 64 mM, 6.98 min(-1) and 0.11 mM(-1) min(-1) respectively.
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Zaiping
authors: Z
authors: Feng
authors: Wanmeng
authors: W
authors: Mu
authors: Bo
authors: B
authors: Jiang
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D005632:Fructose / Q000032:analysis / Q000378:metabolism*; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000201:enzymology* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019216:Metals, Heavy; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D019216:Metals, Heavy; D011994:Recombinant Proteins; C003243:psicose; D005632:Fructose; C002055:allose; D019747:Aldose-Ketose Isomerases; C022548:ribosephosphate isomerase; D005947:Glucose