title: Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source.
abstract: We developed an off-axis-illuminated zone-plate-based hard x-ray Zernike phase-contrast microscope beamline at Pohang Light Source. Owing to condenser optics-free and off-axis illumination, a large field of view was achieved. The pinhole-type Zernike phase plate affords high-contrast images of a cell with minimal artifacts such as the shade-off and halo effects. The setup, including the optics and the alignment, is simple and easy, and allows faster and easier imaging of large bio-samples.
authors: Pohang Accelerator Laboratory, POSTECH, Pohang 790-784, South Korea. limjun@postech.ac.kr
authors: Jun
authors: J
authors: Lim
authors: So Yeong
authors: SY
authors: Park
authors: Jung Yun
authors: JY
authors: Huang
authors: Sung Mi
authors: SM
authors: Han
authors: Hong-Tae
authors: HT
authors: Kim
mesh_terms: D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D008027:Light; D008264:Macrophages / Q000166:cytology; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D012822:Silicon Dioxide / Q000737:chemistry; D014965:X-Rays
chemical_list: D012822:Silicon Dioxide