title: [Determination of phenylethanolamine A residue in feeds by ultra performance liquid chromatography-tandem mass spectrometry].
abstract: A method was developed for the determination of the residue of phenylethanolamine A in feeds by ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). The phenylethanolamine A in sample was extracted with phosphoric acid methanol solution. The extract was purified and concentrated by an MCX column. In the positive mode, an ultra-performance BEH C18 column (100 mm x 2.1 mm, 1.7 microm) was used to separate the phenylethanolamine A, and the separation was carried out on a Waters UPLC system by using gradient elution with acetonitrile and 0.1% (v/v) formic acid aqueous solution as the mobile phases at a flow rate of 0.4 mL/min. The qualitative and quantitative analysis was carried out by multi-reaction monitoring (MRM) mode, in which the analyte was ionized by electrospray ionization interface (ESI). The results showed that, the calibration curve was linear in the range of 0.5-100 microg/kg (r > 0.999), the limit of quantification (S/N > 10) was 10 microg/kg, the recoveries ranged from 79.6% to 86.2%, and the relative standard deviations (RSDs) were between 3.1% and 6.7%. The real sample tests showed that the method is convenient and reliable.
authors: Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China. sunwuyong@sina.com
authors: Wuyong
authors: W
authors: Sun
authors: Binglin
authors: B
authors: Zhao
authors: Shoujie
authors: S
authors: Zhang
authors: Zhuanlian
authors: Z
authors: Sun
authors: Li
authors: L
authors: Zhang
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D004983:Ethanolamines / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000318:Adrenergic beta-Agonists; D004983:Ethanolamines