title: Actinoplanes siamensis sp. nov., isolated from soil.
abstract: A Gram-positive filamentous bacterial strain that developed large campanulate sporangia at the ends of sporangiophores on substrate mycelium was isolated from bamboo forest soil in Thailand. According to the results of a polyphasic taxonomic study, our isolate had typical characteristics of members of the genus Actinoplanes. The 16S rRNA gene sequence analysis also indicated that strain A-T 6646(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes liguriensis DSM 43865(T) (97.61 %) and Actinoplanes octamycinicus NBRC 14524(T) (97.52 %). The DNA-DNA relatedness values, which differentiate the new strain from the most closely related species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained xylose and arabinose. The predominant menaquinone was MK-9(H4). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and anteiso-C15 : 0. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to represent a novel species to be named Actinoplanes siamensis sp. nov. The type strain is A-T 6646(T) (= BCC 46194(T) = NBRC 109076(T)).
authors: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang Pathum Thani 12120, Thailand.
authors: Chanwit
authors: C
authors: Suriyachadkun
authors: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang Pathum Thani 12120, Thailand.
authors: Wipaporn
authors: W
authors: Ngaemthao
authors: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang Pathum Thani 12120, Thailand.
authors: Suwanee
authors: S
authors: Chunhametha
authors: Department of Biology and Actinobacterial Research Unit, Faculty of Science, King Monkut's Institute of Technology Ladkrabang Bangkok, Thailand.
authors: Chitti
authors: C
authors: Thawai
authors: Novartis Institutes for BioMedical Research Basel, Natural Products Unit, Novartis Pharma AG, Basel, Switzerland.|BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang Pathum Thani 12120, Thailand.
authors: Jean-Jacques
authors: JJ
authors: Sanglier
mesh_terms: D015373:Bacterial Typing Techniques; D031723:Bambusa / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9