title: Fluorescence correlation spectroscopy to examine protein-lipid interactions in membranes.
abstract: Fluorescence correlation spectroscopy (FCS) is a versatile technique to study membrane dynamics and protein-lipid interactions. It can provide information about diffusion coefficients, concentrations, and molecular interactions of proteins and lipids in the membrane. These parameters allow the determination of protein partitioning into different lipid environments, the identification of lipid domains, and the detection of lipid-protein complexes on the membrane. During the last decade, FCS studies were successfully performed on model membrane systems as also on living cells, to characterize protein-lipid interactions. Recent developments of the method described here improved quantitative measurements on membranes and decreased the number of potential artifacts. The aim of this chapter is to provide the reader with the necessary information and some practical guidelines to perform FCS studies on artificial and cellular membranes.
authors: Biophysics, BIOTEC, Technische Universitat Dresden, Dresden, Germany.
authors: Viktoria
authors: V
authors: Betaneli
authors: Petra
authors: P
authors: Schwille
mesh_terms: D002470:Cell Survival; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D011485:Protein Binding; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*; D053835:Unilamellar Liposomes / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D012996:Solutions; D053835:Unilamellar Liposomes