title: A stem-loop structure in the 5' untranslated region of bean pod mottle virus RNA2 is specifically required for RNA2 accumulation.
abstract: Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus of the family Secoviridae. Its RNA1 encodes all proteins needed for genome replication and is capable of autonomous replication. By contrast, BPMV RNA2 must utilize RNA1-encoded proteins for replication. Here, we sought to identify RNA elements in RNA2 required for its replication. The exchange of 5' untranslated regions (UTRs) between genome segments revealed an RNA2-specific element in its 5' UTR. Further mapping localized a 66 nucleotide region that was predicted to fold into an RNA stem-loop structure, designated SLC. Additional functional analysis indicated the importance of the middle portion of the stem and an adjacent two-base mismatch. This is the first report of a cis-acting RNA element in RNA2 of a bipartite secovirus.
authors: Department of Plant Pathology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
authors: Junyan
authors: J
authors: Lin
authors: Department of Biology, College of Science, University of Mustansiriyah, Bagdad, Iraq.|Department of Plant Pathology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
authors: Ahmed Khamis
authors: AK
authors: Ali
authors: Key Laboratory of Protection and Utilization of Tropical Crop Germplasm Resources, Hainan University, Haikou, Hainan, People's Republic of China.|Department of Plant Pathology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
authors: Ping
authors: P
authors: Chen
authors: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
authors: Said
authors: S
authors: Ghabrial
authors: Department of Horticulture and Crop Science, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
authors: John
authors: J
authors: Finer
authors: Department of Plant Pathology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
authors: Anne
authors: A
authors: Dorrance
authors: USDA-ARS, Corn and Soybean Research Unit, Wooster, OH 44691, USA.|Department of Plant Pathology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
authors: Peg
authors: P
authors: Redinbaugh
authors: Department of Plant Pathology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
authors: Feng
authors: F
authors: Qu
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D017798:Comovirus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D055029:Inverted Repeat Sequences; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D027805:Phaseolus / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral