title: Arabidopsis ASL11/LBD15 is involved in shoot apical meristem development and regulates WUS expression.
abstract: The ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (ASL/LBD) genes encode plant-specific nuclear proteins containing the conserved domain AS2/LOB. In this study, the function of a member of the Arabidopsis thaliana AS2/LOB gene family, ASL11/LBD15, was investigated. The results show that ASL11/LBD15 is expressed in the meristems of shoot apex, root apex, organ boundaries, and developing seeds. Overexpression of ASL11/LBD15 resulted in aberrant arrangements in the tunica cell layers of the shoot apical meristem (SAM). Two-week-old transgenic plants developed needle-like leaves in addition to regular leaves, while 6-week-old transformants displayed clustered cauline leaves suggesting altered SAM development. qRT-PCR analysis revealed that the WUSCHEL (WUS) transcript level was strongly up-regulated in plants overexpressing ASL11/LBD15 compared with the wild-type plants. Furthermore, inducible ASL11/LBD15 ectopic expression activated ectopic expression of WUS and affected the differentiation of leaf epidermal cells. Therefore, our results suggest that ASL11/LBD15 affects cellular differentiation in the SAM and regulates WUS expression.
authors: Department of Molecular and Cell Biology, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
authors: Xudong
authors: X
authors: Sun
authors: ZhenHua
authors: Z
authors: Feng
authors: LaiSheng
authors: L
authors: Meng
authors: Jian
authors: J
authors: Zhu
authors: Anja
authors: A
authors: Geitmann
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D018519:Meristem / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; C116421:WUSCHEL protein, Arabidopsis
references: Dev Biol. 2004 Sep 15;273(2):321-34
references: 15328016
references: Plant J. 1998 Dec;16(6):735-43
references: 10069079
references: Trends Plant Sci. 2009 Sep;14(9):467-78
references: 19717328
references: Development. 1997 Jan;124(1):33-44
references: 9006065
references: Plant Cell. 2008 Jun;20(6):1482-93
references: 18591352
references: Ann Bot. 2012 Jul;110(1):1-10
references: 22334497
references: Nature. 1996 Jan 4;379(6560):66-9
references: 8538741
references: Annu Rev Cell Dev Biol. 2004;20:125-51
references: 15473837
references: Genes Dev. 2005 Apr 15;19(8):992-1003
references: 15833920
references: Mol Phylogenet Evol. 2006 Apr;39(1):248-62
references: 16290186
references: Plant Mol Biol. 2005 Mar;57(4):559-75
references: 15821980
references: Plant J. 2003 Jun;34(5):741-50
references: 12787254
references: Development. 2001 May;128(10):1771-83
references: 11311158
references: Bioinformatics. 2007 May 15;23(10):1307-8
references: 17392330
references: Plant J. 2007 Jun;50(5):767-81
references: 17461793
references: Plant Physiol. 2002 Jun;129(2):747-61
references: 12068116
references: Plant Cell. 2007 Jan;19(1):118-30
references: 17259263
references: New Phytol. 2005 Apr;166(1):9-20
references: 15760347
references: Curr Opin Plant Biol. 2001 Oct;4(5):441-6
references: 11597503
references: Cell. 2005 Mar 11;120(5):687-700
references: 15766531
references: Plant Cell. 2005 May;17(5):1387-96
references: 15829602
references: Plant Cell. 2006 Aug;18(8):1900-7
references: 16798887
references: Science. 2000 Jul 28;289(5479):617-9
references: 10915624
references: Plant J. 2007 Jul;51(2):173-84
references: 17559509
references: Cell. 2003 Nov 26;115(5):591-602
references: 14651850
references: Plant Cell Physiol. 2002 May;43(5):467-78
references: 12040093
references: BMC Plant Biol. 2011 Feb 01;11:27
references: 21284861
references: Cell. 1998 Dec 11;95(6):805-15
references: 9865698
references: Plant J. 2009 Aug;59(3):448-60
references: 19453451
references: Plant Cell. 2008 Jan;20(1):48-58
references: 18203921
references: Development. 2003 Sep;130(17):4097-107
references: 12874130
references: Plant Mol Biol. 2005 Apr;57(6):773-84
references: 15952065
references: Plant Physiol. 2012 Sep;160(1):407-18
references: 22786889
references: Nature. 2007 Apr 12;446(7137):811-4
references: 17429400
references: Plant J. 2004 Apr;38(1):164-71
references: 15053769
references: Plant J. 2009 May;58(3):525-37
references: 19154202
references: Nat Protoc. 2006;1(3):1462-7
references: 17406436
references: Plant Cell. 2012 Jul;24(7):2917-33
references: 22822207
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Dev Biol. 2009 Dec 1;336(1):1-9
references: 19782675
references: Plant Cell. 2000 Nov;12(11):2161-74
references: 11090216
references: Curr Opin Plant Biol. 2006 Feb;9(1):72-7
references: 16337829
references: Science. 2006 Aug 11;313(5788):773-4
references: 16902118
references: Genes Dev. 2004 Feb 15;18(4):375-80
references: 15004006
references: Nature. 2005 Dec 22;438(7071):1172-5
references: 16372013
references: Plant Cell. 2003 Oct;15(10):2241-52
references: 14508003
references: Nucleic Acids Res. 2007;35(19):6663-71
references: 17913740
references: Plant Cell. 2007 Jun;19(6):1795-808
references: 17557810
references: Biosci Biotechnol Biochem. 2007 May;71(5):1269-78
references: 17485849
references: Cell. 2000 Mar 17;100(6):635-44
references: 10761929
references: Plant Cell. 2006 May;18(5):1188-98
references: 16603652
references: Plant Physiol. 2009 Nov;151(3):1377-89
references: 19717544
references: Cell Res. 2012 Jul;22(7):1169-80
references: 22508267