title: Exploring the diversity of Arcobacter butzleri from cattle in the UK using MLST and whole genome sequencing.
abstract: Arcobacter butzleri is considered to be an emerging human foodborne pathogen. The completion of an A. butzleri genome sequence along with microarray analysis of 13 isolates in 2007 revealed a surprising amount of diversity amongst A. butzleri isolates from humans, animals and food. In order to further investigate Arcobacter diversity, 792 faecal samples were collected from cattle on beef and dairy farms in the North West of England. Arcobacter was isolated from 42.5% of the samples and the diversity of the isolates was investigated using multilocus sequence typing. An A. butzleri whole genome sequence, obtained by 454 shotgun sequencing of an isolate from a clinically-healthy dairy cow, showed a number of differences when compared to the genome of a human-derived A. butzleri isolate. PCR-based prevalence assays for variable genes suggested some tentative evidence for source-related distributions. We also found evidence for phenotypic differences relating to growth capabilities between our representative human and cattle isolates. Our genotypic and phenotypic observations suggest that some level of niche adaptation may have occurred in A. butzleri.
authors: Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom. ysloane@liv.ac.uk
authors: J Yvette
authors: JY
authors: Merga
authors: Nicola J
authors: NJ
authors: Williams
authors: William G
authors: WG
authors: Miller
authors: Andrew J H
authors: AJ
authors: Leatherbarrow
authors: Malcolm
authors: M
authors: Bennett
authors: Neil
authors: N
authors: Hall
authors: Kevin E
authors: KE
authors: Ashelford
authors: Craig
authors: C
authors: Winstanley
mesh_terms: D000818:Animals; D020640:Arcobacter / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004739:England; D005243:Feces / Q000382:microbiology; D055106:Genome-Wide Association Study / Q000379:methods; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D017422:Sequence Analysis, DNA / Q000379:methods
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