title: Making proteins green; biosynthesis of chlorophyll-binding proteins in cyanobacteria.
abstract: Chlorophyll (Chl) is an essential component of the photosynthetic apparatus. Embedded into Chl-binding proteins, Chl molecules play a central role in light harvesting and charge separation within the photosystems. It is critical for the photosynthetic cell to not only ensure the synthesis of a sufficient amount of new Chl-binding proteins but also avoids any misbalance between apoprotein synthesis and the formation of potentially phototoxic Chl molecules. According to the available data, Chl-binding proteins are translated on membrane bound ribosomes and their integration into the membrane is provided by the SecYEG/Alb3 translocon machinery. It appears that the insertion of Chl molecules into growing polypeptide is a prerequisite for the correct folding and finishing of Chl-binding protein synthesis. Although the Chl biosynthetic pathway is fairly well-described on the level of enzymatic steps, a link between Chl biosynthesis and the synthesis of apoproteins remains elusive. In this review, I summarize the current knowledge about this issue putting emphasis on protein-protein interactions. I present a model of the Chl biosynthetic pathway organized into a multi-enzymatic complex and physically attached to the SecYEG/Alb3 translocon. Localization of this hypothetical large biosynthetic centre in the cyanobacterial cell is also discussed as well as regulatory mechanisms coordinating the rate of Chl and apoprotein synthesis.
authors: Institute of Microbiology CAS, Opatovický mlyn, Třeboň, Czech Republic, sobotka@alga.cz.
authors: Roman
authors: R
authors: Sobotka
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D061146:Chlorophyll Binding Proteins / Q000096:biosynthesis*; D000458:Cyanobacteria / Q000166:cytology / Q000378:metabolism*; D010788:Photosynthesis
chemical_list: D001426:Bacterial Proteins; D061146:Chlorophyll Binding Proteins; D002734:Chlorophyll
references: Biochim Biophys Acta. 2007 Jul;1767(7):920-9
references: 17499209
references: Science. 2003 Feb 7;299(5608):902-6
references: 12574634
references: Proc Natl Acad Sci U S A. 1990 Jun;87(11):4038-42
references: 2349216
references: Plant Cell Environ. 2012 Apr;35(4):806-18
references: 22070528
references: J Biol Chem. 2001 Oct 12;276(41):37809-14
references: 11473124
references: Plant Cell. 2006 Jun;18(6):1454-66
references: 16679460
references: J Biol Chem. 2005 Sep 9;280(36):31595-602
references: 16027152
references: Microbiology (Reading). 2009 Mar;155(Pt 3):989-996
references: 19246769
references: FEBS J. 2009 Mar;276(5):1398-417
references: 19187240
references: FEBS J. 2005 Sep;272(17):4532-9
references: 16128821
references: Microbiology (Reading). 2008 Dec;154(Pt 12):3707-3714
references: 19047738
references: J Biol Chem. 2008 Sep 19;283(38):25794-802
references: 18625715
references: Planta. 2003 Jan;216(3):475-83
references: 12520340
references: Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6860-5
references: 19342483
references: Ann Bot. 2010 Jul;106(1):1-16
references: 20338950
references: Plant Physiol. 2011 Apr;155(4):1735-47
references: 21081693
references: J Cell Biol. 2003 Sep 29;162(7):1245-54
references: 14517205
references: Biochemistry. 2005 Aug 16;44(32):10864-72
references: 16086589
references: Mol Microbiol. 2002 Jul;45(1):17-29
references: 12100545
references: Annu Rev Plant Biol. 2007;58:321-46
references: 17227226
references: Biochim Biophys Acta. 2005 Jun 1;1708(1):91-101
references: 15949987
references: J Biol Chem. 2012 Aug 10;287(33):27823-33
references: 22711541
references: Mol Cell. 2008 Feb 29;29(4):419-27
references: 18313381
references: FEBS Lett. 2012 Feb 3;586(3):211-6
references: 22212719
references: J Biol Chem. 2007 Dec 28;282(52):37660-8
references: 17971445
references: Plant Cell. 2012 Feb;24(2):660-75
references: 22319052
references: Mol Cell. 2009 May 15;34(3):344-53
references: 19450532
references: J Exp Bot. 2012 Feb;63(4):1675-87
references: 22231500
references: Plant Cell Physiol. 2010 May;51(5):670-81
references: 20375109
references: Annu Rev Biochem. 2011;80:161-87
references: 21275640
references: Mol Biol Cell. 2012 Feb;23(3):464-79
references: 22160593
references: J Biol Chem. 2011 Oct 7;286(40):35187-95
references: 21832051
references: Eur J Biochem. 1995 Oct 15;233(2):677-82
references: 7588816
references: FEBS Lett. 1992 Dec 7;314(1):77-80
references: 1451806
references: J Bacteriol. 2008 Mar;190(6):2086-95
references: 18192382
references: FEBS Lett. 2005 Aug 29;579(21):4808-12
references: 16109415
references: Biochim Biophys Acta. 2011 Sep;1807(9):1143-51
references: 21605542
references: Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19286-91
references: 23129655
references: J Biol Chem. 2005 May 13;280(19):18568-72
references: 15757895
references: J Biol Chem. 1996 Dec 13;271(50):32174-9
references: 8943272
references: Plant Cell Physiol. 2010 May;51(5):823-35
references: 20231245
references: Plant Cell. 1999 Dec;11(12):2365-77
references: 10590164
references: Annu Rev Plant Biol. 2004;55:373-99
references: 15377225
references: Microbiol Mol Biol Rev. 2007 Mar;71(1):230-53
references: 17347523
references: Mol Microbiol. 2001 Apr;40(2):476-84
references: 11309129
references: Plant Physiol. 1998 Aug;117(4):1205-16
references: 9701577
references: Appl Microbiol Biotechnol. 2005 Dec;69(4):440-7
references: 15988575
references: Mol Microbiol. 2012 Jul;85(1):21-38
references: 22624875
references: J Biol Chem. 2004 Dec 31;279(53):55792-800
references: 15498761
references: Plant Cell. 2006 Nov;18(11):3121-31
references: 17114356
references: Plant Cell. 2011 Apr;23(4):1449-67
references: 21467578
references: Curr Opin Plant Biol. 2012 Jun;15(3):245-51
references: 22386092
references: J Cell Biol. 2004 Apr;165(1):53-62
references: 15067017
references: Nature. 1999 Dec 2;402(6761 Suppl):C47-52
references: 10591225
references: FEBS Lett. 2002 Jul 31;524(1-3):127-33
references: 12135754
references: J Microbiol Biotechnol. 2008 Jun;18(6):1090-4
references: 18600052
references: Planta. 2013 Feb;237(2):497-508
references: 23011568
references: Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):636-40
references: 7831342
references: J Biol Chem. 2006 Oct 27;281(43):32705-13
references: 16923804
references: Biochemistry. 2005 May 31;44(21):7603-12
references: 15909975
references: J Biol Chem. 2012 Jan 2;287(1):682-692
references: 22090028
references: Biochem J. 2002 Dec 15;368(Pt 3):777-81
references: 12217076
references: J Proteome Res. 2011 Aug 5;10(8):3617-31
references: 21648951
references: J Biol Chem. 2011 Nov 25;286(47):41057-68
references: 21965654
references: J Biol Chem. 2005 Jul 1;280(26):24301-7
references: 15890644
references: Mol Microbiol. 1995 Jun;16(6):1051-7
references: 8577241
references: J Biol Chem. 1994 Jul 8;269(27):17918-23
references: 8027048
references: Photosynth Res. 2010 Nov;106(1-2):57-71
references: 20596891
references: Plant J. 2007 Jun;50(5):795-809
references: 17553115
references: J Bacteriol. 1987 Jun;169(6):2718-23
references: 3294804
references: Plant Physiol. 2006 Nov;142(3):911-22
references: 17028153
references: J Cell Biol. 1976 Nov;71(2):497-514
references: 993261
references: Plant J. 2008 Dec;56(6):1007-17
references: 18764927
references: Plant Physiol. 2012 Dec;160(4):2239-50
references: 23037506
references: Photochem Photobiol Sci. 2008 Oct;7(10):1131-49
references: 18846277
references: Plant Physiol. 2012 May;159(1):118-30
references: 22452855
references: Plant Cell. 2006 Sep;18(9):2236-46
references: 16905652