title: Role of nitric oxide in the control of the gastric motility within the nucleus ambiguus of rats.
abstract: This study aims to investigate whether exogenous nitric oxide (NO) plays a role in controlling gastric motility within the nucleus ambiguus (NA). Experiments were performed on male Wistar rats anaesthetized with chloral hydrate. A latex balloon, connected to a pressure transducer, was inserted into the pylorus through the fundus for continuous recording of the change of gastric smooth muscle contractile curves. Microinjection of the NO-donor sodium nitroprusside (SNP; 5 nmol) or L-arginine (L-Arg; 5 nmol) into the NA significantly inhibited gastric motility, whereas the treatment of NO-synthase inhibitor N-nitro-L-arginine methylester (L-NAME) increased gastric motility remarkably. The negative effect of SNP or L-Arg on gastric motility was abolished by bilateral subdiaphragmatic vagotomy as well as by intravenous injection of ganglionic blocker, hexamethonium bromide (Hb). These results demonstrated that NO inhibited gastric motility by activating the cholinergic preganglionic neurons in the NA and through the mediation of vagus nerves.
authors: Biological Department, Qi Lu Normal University, Jinan, China. sunhongzhao18@126.com
authors: H-Z
authors: HZ
authors: Sun
authors: S-Z
authors: SZ
authors: Zhao
authors: H-B
authors: HB
authors: Ai
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage; D004562:Electrocardiography; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005730:Ganglionic Blockers / Q000494:pharmacology; D005769:Gastrointestinal Motility / Q000187:drug effects; D006339:Heart Rate; D018738:Hexamethonium / Q000008:administration & dosage; D007275:Injections, Intravenous; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects / Q000378:metabolism*; D008845:Microinjections; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000008:administration & dosage; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009599:Nitroprusside / Q000008:administration & dosage; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects / Q000294:innervation*; D014628:Vagotomy; D014630:Vagus Nerve / Q000187:drug effects / Q000378:metabolism* / Q000601:surgery
chemical_list: D004791:Enzyme Inhibitors; D005730:Ganglionic Blockers; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D018738:Hexamethonium; D001120:Arginine; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester