title: Gigapixel fluorescence microscopy with a water immersion microlens array.
abstract: We demonstrate high throughput gigapixel fluorescence microscopy with a microlens array. We show, for the first time to the best of our knowledge, the use of a parallelized microscopy system to image samples in micro well plates. We image centimeter-scale regions of 384-well micro well plates at 1.72 μm resolution at a raw pixel throughput of 25.4 Mpx/s. Taking into account the fact that about half the well plate area consists of the plastic support region between wells, this corresponds to a sample pixel throughput of 13.2 Mpx/s, more than double that of the commercial state-of-the-art at the time of writing. Fluorescent imaging of tissue samples through coverslips is also demonstrated.
authors: School of Engineering and Applied Sciences, Harvard University, 33 Oxford St., Cambridge, Massachusetts 02138, USA. tonyorth@seas.harvard.edu
authors: Antony
authors: A
authors: Orth
authors: Kenneth
authors: K
authors: Crozier
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D044085:Microfluidics / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D008904:Miniaturization; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D014867:Water
chemical_list: D014867:Water