title: A high-resolution enhancer atlas of the developing telencephalon.
abstract: The mammalian telencephalon plays critical roles in cognition, motor function, and emotion. Though many of the genes required for its development have been identified, the distant-acting regulatory sequences orchestrating their in vivo expression are mostly unknown. Here, we describe a digital atlas of in vivo enhancers active in subregions of the developing telencephalon. We identified more than 4,600 candidate embryonic forebrain enhancers and studied the in vivo activity of 329 of these sequences in transgenic mouse embryos. We generated serial sets of histological brain sections for 145 reproducible forebrain enhancers, resulting in a publicly accessible web-based data collection comprising more than 32,000 sections. We also used epigenomic analysis of human and mouse cortex tissue to directly compare the genome-wide enhancer architecture in these species. These data provide a primary resource for investigating gene regulatory mechanisms of telencephalon development and enable studies of the role of distant-acting enhancers in neurodevelopmental disorders.
authors: Genomics Division, MS 84-171, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. avisel@lbl.gov
authors: Axel
authors: A
authors: Visel
authors: Leila
authors: L
authors: Taher
authors: Hani
authors: H
authors: Girgis
authors: Dalit
authors: D
authors: May
authors: Olga
authors: O
authors: Golonzhka
authors: Renee V
authors: RV
authors: Hoch
authors: Gabriel L
authors: GL
authors: McKinsey
authors: Kartik
authors: K
authors: Pattabiraman
authors: Shanni N
authors: SN
authors: Silberberg
authors: Matthew J
authors: MJ
authors: Blow
authors: David V
authors: DV
authors: Hansen
authors: Alex S
authors: AS
authors: Nord
authors: Jennifer A
authors: JA
authors: Akiyama
authors: Amy
authors: A
authors: Holt
authors: Roya
authors: R
authors: Hosseini
authors: Sengthavy
authors: S
authors: Phouanenavong
authors: Ingrid
authors: I
authors: Plajzer-Frick
authors: Malak
authors: M
authors: Shoukry
authors: Veena
authors: V
authors: Afzal
authors: Tommy
authors: T
authors: Kaplan
authors: Arnold R
authors: AR
authors: Kriegstein
authors: Edward M
authors: EM
authors: Rubin
authors: Ivan
authors: I
authors: Ovcharenko
authors: Len A
authors: LA
authors: Pennacchio
authors: John L R
authors: JL
authors: Rubenstein
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005333:Fetus / Q000378:metabolism; D055106:Genome-Wide Association Study; D006801:Humans; D051379:Mice; D013687:Telencephalon / Q000196:embryology / Q000378:metabolism*; D059467:Transcriptome; D050880:p300-CBP Transcription Factors / Q000378:metabolism
chemical_list: D050880:p300-CBP Transcription Factors
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