title: Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori.
abstract: This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori to analyse intra-familial transmission. Faecal DNA was extracted and amplified by nested PCR. The products were analysed by direct sequencing and the allele type was determined using an MLST website. Mother-to-child transmission was suspected in at least two of three families, and father-to-child transmission was suspected in one family.
authors: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
authors: Takako
authors: T
authors: Osaki
authors: Department of General Medicine and Community Health Science, Hyogo College of Medicine, Hyogo, Japan.
authors: Masumi
authors: M
authors: Okuda
authors: Department of Public Health, Aichi Medical University School of Medicine, Aichi, Japan.
authors: Junko
authors: J
authors: Ueda
authors: Department of Pediatrics, Sapporo Kosei General Hospital, Sapporo, Japan.
authors: Mutsuko
authors: M
authors: Konno
authors: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
authors: Hideo
authors: H
authors: Yonezawa
authors: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
authors: Fuhito
authors: F
authors: Hojo
authors: Department of Public Health, Aichi Medical University School of Medicine, Aichi, Japan.
authors: Kiyoko
authors: K
authors: Yagyu
authors: Department of Public Health, Aichi Medical University School of Medicine, Aichi, Japan.
authors: Yingsong
authors: Y
authors: Lin
authors: Department of General Medicine and Community Health Science, Hyogo College of Medicine, Hyogo, Japan.
authors: Yoshihiro
authors: Y
authors: Fukuda
authors: Department of Public Health, Aichi Medical University School of Medicine, Aichi, Japan.
authors: Shogo
authors: S
authors: Kikuchi
authors: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
authors: Shigeru
authors: S
authors: Kamiya
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D005190:Family; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D005260:Female; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007564:Japan / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing / Q000379:methods*
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial