title: Dextranase: hyper production of dextran degrading enzyme from newly isolated strain of Bacillus licheniformis.
abstract: Dextranase, 6-alpha-D-glucan 6-glucanohydrolase catalyzes the degradation of dextran (polymer of D-glucose) in to low molecular weight fractions. Dextranolytic bacterial strains were isolated from various natural sources and plate assay methods were developed for screening of highest extracellular dextranase producing isolate. Bacillus licheniformis, identified on the basis of taxonomic characterization was subjected to UV radiation and highest enzyme producing mutant obtained led to 7 times more dextranase production than wild. Optimization of major physico-chemical parameters affecting enzyme production; including medium composition, pH, cultivation time and temperature revealed that maximum enzyme production was obtained in a self designed medium (pH 6.0) containing 1% Dextran 5000 Da, after 24 h culture incubation at 37 °C. Dextranase reported in this study is of great commercial importance as it is strictly inducible in nature and B. licheniformis being non-pathogenic removes the safety concerns associated with production of dextran fractions for clinical and pharmaceutical usage.
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors: Rashida Rahmat
authors: RR
authors: Zohra
authors: Afsheen
authors: A
authors: Aman
authors: Raheela Rahmat
authors: RR
authors: Zohra
authors: Asma
authors: A
authors: Ansari
authors: Maria
authors: M
authors: Ghani
authors: Shah Ali Ul
authors: SA
authors: Qader
mesh_terms: D001407:Bacillus / Q000166:cytology / Q000201:enzymology* / Q000302:isolation & purification* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D003910:Dextranase / Q000096:biosynthesis* / Q000378:metabolism*; D003911:Dextrans / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D009154:Mutation / Q000528:radiation effects; D009584:Nitrogen / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D003911:Dextrans; D002244:Carbon; D003910:Dextranase; D009584:Nitrogen