title: Computational analysis of bovine alpha-1 collagen sequences.
abstract: Bovine collagen alpha-1 is a naturally occurring extracellular matrix protein found in tendons and other connective tissues. It plays a vital role in cell growth, differentiation, attachment, and migration. Recent findings have established that collagen alpha-1 is involved in osteogenesis imperfecta phenotype in cattle but deep information about other members of this large family is not available so far. So with a view to finding a new edge and attempt to figure out a correlation among the well attributed Bovine alpha-1 collagen sequences are executed and analyzed. To do so, comparative analysis among the 28 members of collagen family has been carried out using Computational tools. Consequently, based on the physico-chemical, secondary structural, functional and phylogenetic classifications, we have selected collagen 12, 14 and 20 as targets for pathological conditions. These proteins belong to the FACIT family and significantly showed low glycine and proline content, high instability and aliphatic index. Moreover, FACIT family collagens contain multiple triple helical domains and being members of the FACIT family, bovine collagen 12, 14, 20 do not form fibrils by themselves but they are associated to collagen 1 associated fibrils. These collagen molecules might be crucial candidates to detect and understand the process of matrix remodeling in diseases especially in the arena of cellular compartments.
authors: Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh.
authors: Ayan
authors: A
authors: Saha
authors: Md Jibran
authors: MJ
authors: Alam
authors: Kutub Uddin Muhammad
authors: KU
authors: Ashraf
authors: Adnan
authors: A
authors: Mannan
keywords: Collagen; Extracellular matrix; computational tools
references: Bioinformation. 2011 Mar 02;6(1):23-30
references: 21464841
references: FASEB J. 1991 Oct;5(13):2814-23
references: 1916105
references: J Mol Cell Cardiol. 2010 Mar;48(3):474-82
references: 19729019
references: Nucleic Acids Res. 2003 Jul 1;31(13):3784-8
references: 12824418
references: Comput Appl Biosci. 1995 Dec;11(6):681-4
references: 8808585
references: Nucleic Acids Res. 2007 Jul;35(Web Server issue):W433-7
references: 17545200
references: Pharmacol Rev. 2009 Jun;61(2):198-223
references: 19549927
references: Biochim Biophys Acta. 1973 Sep 21;322(1):166-71
references: 4744330
references: Zentralbl Veterinarmed A. 1994 Mar;41(2):128-38
references: 8091888
references: Biochem J. 1996 May 15;316 ( Pt 1):1-11
references: 8645190
references: Am J Hum Genet. 2001 Nov;69(5):969-80
references: 11565064