title: Characterization of a Novel Panton-Valentine leukocidin (PVL)-encoding staphylococcal phage and its naturally PVL-lacking variant.
abstract: A new siphophage (LH1) was isolated from raw milk using a Staphylococcus aureus ST352 host. Its genome (46,048 bp, 57 open reading frames) includes the two genes encoding Panton-Valentine leukocidin (PVL), a virulence factor usually harbored by S. aureus prophages. Nine structural proteins were identified, including a tail protein generated through a +1 frameshift. A phage lytic mutant was isolated, and its analysis revealed the deletion of genes coding for the PVL and an integrase. The deletion likely occurred through recombination between direct repeats.
authors: Département de biochimie, microbiologie et bio-informatique, Faculté des sciences et de génie, Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Félix d'Hérelle Reference Center for Bacterial Viruses, Université Laval, Québec, Canada.
authors: Lynn
authors: L
authors: El Haddad
authors: Sylvain
authors: S
authors: Moineau
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005098:Exotoxins / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D007956:Leukocidins / Q000235:genetics*; D008892:Milk / Q000821:virology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013204:Staphylococcus Phages / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000821:virology*
chemical_list: D001427:Bacterial Toxins; D004279:DNA, Viral; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin
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