title: Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs.
abstract: There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.
authors: Immunology Division, Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa. vanwyngaardtw@arc.agric.za
authors: Wouter
authors: W
authors: van Wyngaardt
authors: Cordelia
authors: C
authors: Mashau
authors: Isabel
authors: I
authors: Wright
authors: Jeanni
authors: J
authors: Fehrsen
mesh_terms: D000356:African Horse Sickness Virus / Q000302:isolation & purification*; D000818:Animals; D055910:Antibodies, Immobilized; D000914:Antibodies, Viral / Q000276:immunology*; D002645:Chickens; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G; D019151:Peptide Library; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary; D012703:Serotyping; D057127:Single-Chain Antibodies / Q000276:immunology*; D014709:Vero Cells
chemical_list: D055910:Antibodies, Immobilized; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D019151:Peptide Library; D057127:Single-Chain Antibodies
keywords: African horsesickness virus; double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); phage-displayed scFv; serotype-specific
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