title: Immobilization of bovine serum albumin-protected gold nanoclusters by using polyelectrolytes of opposite charges for the development of the reusable fluorescent Cu2+-sensor.
abstract: In this paper, we reported a facile method of fabricating a reusable fluorescent Cu(2+)-sensor. To fabricate the reusable sensor, the bovine serum albumin-protected gold nanoclusters (BSAGNCs) were complexed with polyelectrolytes, i.e., positively charged polydiallyldimethylammonium (PDDA) and negatively charged polystyrenesulfonate (PSS), and were coated on a glass slide. The prepared film-modified glass slides exhibited the recyclability of fluorescent signal "off-on" behaviors: the fluorescence could be switched "off" by immersing the film sensors in Cu(2+) solution and "on" by washing with ethylene diamine tetraacetic acid (EDTA) solution. The prepared film-modified glass slides exhibited high selectivity towards Cu(2+) the fluorescence quenching behavior in the form of the Stern-Volmer equation. In addition, the spiked tap water samples were analyzed with satisfactory results. These demonstrations provide a new convenient approach to develop the BSAGNCs-based, reusable fluorescence sensors.
authors: Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, China.
authors: Lei
authors: L
authors: Su
authors: Tong
authors: T
authors: Shu
authors: Zongwei
authors: Z
authors: Wang
authors: Jingya
authors: J
authors: Cheng
authors: Feng
authors: F
authors: Xue
authors: Chenzhong
authors: C
authors: Li
authors: Xueji
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000032:analysis; D002417:Cattle; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013451:Sulfonic Acids / Q000737:chemistry; D014867:Water / Q000032:analysis
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; D014867:Water; D012710:Serum Albumin, Bovine; D003300:Copper