title: Methylotrophic methanogenic Thermoplasmata implicated in reduced methane emissions from bovine rumen.
abstract: Rumen methanogens are major sources of anthropogenic methane emissions, and these archaea are targets in strategies aimed at reducing methane emissions. Here we show that the poorly characterised Thermoplasmata archaea in bovine rumen are methylotrophic methanogens and that they are reduced upon dietary supplementation with rapeseed oil in lactating cows. In a metatranscriptomic survey, Thermoplasmata 16S rRNA and methyl-coenzyme M reductase (mcr) transcripts decreased concomitantly with mRNAs of enzymes involved in methanogenesis from methylamines that were among the most abundant archaeal transcripts, indicating that these Thermoplasmata degrade methylamines. Their methylotrophic methanogenic lifestyle was corroborated by in vitro incubations, showing enhanced growth of these organisms upon methylamine supplementation paralleled by elevated methane production. The Thermoplasmata have a high potential as target in future strategies to mitigate methane emissions from ruminant livestock. Our findings and the findings of others also indicate a wider distribution of methanogens than previously anticipated.
authors: Department of Animal Science, Aarhus University, Blichers allé 20, 8830 Tjele, Denmark. Morten.Poulsen@agrsci.dk
authors: Morten
authors: M
authors: Poulsen
authors: Clarissa
authors: C
authors: Schwab
authors: Bent Borg
authors: BB
authors: Jensen
authors: Ricarda M
authors: RM
authors: Engberg
authors: Anja
authors: A
authors: Spang
authors: Nuria
authors: N
authors: Canibe
authors: Ole
authors: O
authors: Højberg
authors: Gabriel
authors: G
authors: Milinovich
authors: Lena
authors: L
authors: Fragner
authors: Christa
authors: C
authors: Schleper
authors: Wolfram
authors: W
authors: Weckwerth
authors: Peter
authors: P
authors: Lund
authors: Andreas
authors: A
authors: Schramm
authors: Tim
authors: T
authors: Urich
mesh_terms: D000818:Animals; D002417:Cattle; D019587:Dietary Supplements; D019605:Euryarchaeota / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated; D016013:Likelihood Functions; D054892:Metagenome / Q000187:drug effects; D008697:Methane / Q000378:metabolism*; D008744:Methylamines / Q000378:metabolism; D058458:Nitrogen Cycle / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D010938:Plant Oils / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000074262:Rapeseed Oil; D012417:Rumen / Q000187:drug effects / Q000382:microbiology*; D059467:Transcriptome / Q000235:genetics
chemical_list: D005229:Fatty Acids, Monounsaturated; D008744:Methylamines; D010938:Plant Oils; D012333:RNA, Messenger; D000074262:Rapeseed Oil; D008697:Methane
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