title: Light-switchable polymer from cationic to zwitterionic form: synthesis, characterization, and interactions with DNA and bacterial cells.
abstract: A novel cationic polymer poly(N,N-dimethyl-N-[3-(methacroylamino) propyl]-N-[2-[(2-nitrophenyl)methoxy]-2-oxo-ethyl]ammonium chloride) is synthesized by free-radical polymerization of N-[3-(dimethylamino)propyl] methacrylamide and subsequent quaternization with o-nitrobenzyl 2-chloroacetate. The photolabile o-nitrobenzyl carboxymethyl pendant moiety is transformed to the zwitterionic carboxybetaine form upon the irradiation at 365 nm. This feature is used to condense and, upon the light irradiation, to release double-strand DNA tested by gel electrophoresis and surface plasmon resonance experiments as well as to switch the antibacterial activity to non-toxic character demonstrated for Escherichia coli bacterial cells in solution and at the surface using the self-assembled monolayers.
authors: Polymer Institute of the Slovak Academy of Sciences, Center of Excellence FUN-MAT, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
authors: Patrik
authors: P
authors: Sobolčiak
authors: Mário
authors: M
authors: Spírek
authors: Jaroslav
authors: J
authors: Katrlík
authors: Peter
authors: P
authors: Gemeiner
authors: Igor
authors: I
authors: Lacík
authors: Peter
authors: P
authors: Kasák
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005609:Free Radicals / Q000737:chemistry; D008027:Light; D010782:Photolysis; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D000178:Acrylamides; D002412:Cations; D005609:Free Radicals; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D004247:DNA; C045985:methacrylamide