title: Establishment of a new representative model of human ovarian cancer in mice.
abstract: BACKGROUND
  Intraperitoneal (i.p.) models that accurately mimic the feature behavior of human ovarian cancer are required to investigate the pathology and therapeutics of the disease. However, established i.p. models which are well-characterized and reliable are few. The purposes of this study are to establish a representative mice i.p. model of the disease and to analyze the consequent pathology.
  
  
  METHODS
  Fresh tumor cells fiom the ascites of patient were injected into female NOD/SCID mice intraperitoneally. Histology, Cytogenetic, immunohistochemistry,tumor markers of CA125,AFP, CA-199 and CEA were used to analyze the model.
  
  
  RESULTS
  The mice developed marked abdominal distention within 6 months after inoculated with tumor cells from a patient with epithelial ovarian carcinoma. The mice developed clinically evident intraperitoneal tumors and massive ascites containing numerous tumor cells in clumps. CA125 level in our model was high in both serum and ascites supernatants, while levels of other tumor markers, such as AFP, CA-199 and CEA, were normal. Cytogenetic analysis and immunohistochemical staining confirmed its characteristics resembling human epithelial ovarian tumor.
  
  
  CONCLUSIONS
  The model described in this paper accurately mimics the features of ovarian tumor, which may be useful for evaluation of new therapeutics.
authors: Laboratory of Genetics, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P, R, China. z.chen2000@yahoo.cn.
authors: Jianjun
authors: J
authors: Zhang
authors: Xinlian
authors: X
authors: Chen
authors: Gang
authors: G
authors: Shi
authors: Xiaoyan
authors: X
authors: Xie
authors: Hongqian
authors: H
authors: Liu
authors: Xuemei
authors: X
authors: Zhang
authors: Yi
authors: Y
authors: Lai
authors: Yan
authors: Y
authors: Zuo
authors: Zhong
authors: Z
authors: Chen
authors: Shanling
authors: S
authors: Liu
authors: He
authors: H
authors: Wang
references: Lancet. 1971 Oct 30;2(7731):971-2
references: 4107917
references: Int J Cancer. 1989 Sep 15;44(3):494-500
references: 2777413
references: J Exp Clin Cancer Res. 2007 Dec;26(4):467-74
references: 18365540
references: Cancer Res. 1987 May 15;47(10):2662-7
references: 3567897
references: Gynecol Oncol. 2006 Oct;103(1):315-20
references: 16677689
references: Am J Pathol. 1991 Apr;138(4):799-806
references: 1707232
references: Mol Ther. 2004 Dec;10(6):1032-42
references: 15564135
references: Exp Mol Pathol. 1997 Apr;64(2):103-13
references: 9316588
references: Int J Gynecol Pathol. 1999 Jan;18(1):29-41
references: 9891239
references: Int J Cancer. 1996 Nov 27;68(5):588-95
references: 8938139
references: Gynecol Oncol. 2002 Jan;84(1):126-34
references: 11748988
references: Am J Pathol. 2000 Feb;156(2):409-17
references: 10666369
references: Clin Cancer Res. 2003 Feb;9(2):845-52
references: 12576458
references: Gynecol Oncol. 2004 May;93(2):301-6
references: 15099937
references: Eur J Gynaecol Oncol. 1995;16(3):181-6
references: 7664763
references: Eur J Surg Oncol. 2011 Jan;37(1):4-9
references: 21112721
references: Gynecol Oncol. 2002 Nov;87(2):200-6
references: 12477452
references: Cancer Lett. 2011 Apr 1;303(1):65-71
references: 21316839
references: Cancer Res. 1984 Nov;44(11):5286-90
references: 6333272
references: Acta Obstet Gynecol Scand. 1978;57(2):149-59
references: 636856
references: Clin Exp Immunol. 1995 Sep;101(3):494-501
references: 7664496
references: CA Cancer J Clin. 2010 Sep-Oct;60(5):277-300
references: 20610543
references: Acta Obstet Gynecol Scand. 1977;56(1):55-9
references: 402784
references: Eur J Nucl Med. 1996 May;23(5):560-7
references: 8698062
references: Int J Oncol. 1999 Dec;15(6):1155-62
references: 10568822
references: Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4961-5
references: 8506342
references: Curr Opin Cell Biol. 1995 Oct;7(5):728-35
references: 8573349
references: Int J Exp Pathol. 1996 Oct;77(5):219-27
references: 8977374