title: Small ruminant lentiviruses in Jordan: evaluation of sheep and goat serological response using recombinant and peptide antigens.
abstract: Small ruminant lentiviruses infect sheep and goats worldwide, causing chronic progressive diseases and relevant economic losses. Disease eradication and prevention is mostly based on serological testing. The goal of this research was to investigate the presence of the small ruminant lentiviruses (SRLVs) in Jordan and to characterize the serological response in sheep and goat populations. A panel of sera were collected from flocks located in Northern Jordan and Jordan Valley. The samples were tested using three ELISA assays: a commercially available ELISA based on p25 recombinant protein and transmembrane peptide derived from British maedi-visna virus (MVV) EV1 strain, an ELISA based on P16-P25 recombinant protein derived from two Italian strains representative of MVV- and caprine arthritis encephalitis virus (CAEV)-like SRLVs, and an ELISA based on SU5 peptide from the same two Italian isolates. The results indicate that both MVV- and CAEV-like strains are present in Jordan and that the majority of the viruses circulating among sheep and goat populations belong to the MVV-like genotype.
authors: Dipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge, 2, 56124 Pisa, Italy.
authors: Francesco
authors: F
authors: Tolari
authors: Wafa'a
authors: W
authors: Al-Ramadneh
authors: Maurizio
authors: M
authors: Mazzei
authors: Maria Luisa
authors: ML
authors: Carrozza
authors: Mario
authors: M
authors: Forzan
authors: Patrizia
authors: P
authors: Bandecchi
authors: Elena
authors: E
authors: Grego
authors: Sergio
authors: S
authors: Rosati
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D016088:Arthritis-Encephalitis Virus, Caprine / Q000145:classification / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015511:Goat Diseases / Q000453:epidemiology / Q000821:virology*; D006041:Goats; D007597:Jordan / Q000453:epidemiology; D016180:Lentivirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000453:epidemiology / Q000821:virology; D015995:Prevalence; D011994:Recombinant Proteins / Q000276:immunology; D016036:Seroepidemiologic Studies; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000821:virology*; D016182:Visna / Q000453:epidemiology / Q000821:virology; D014790:Visna-maedi virus / Q000145:classification / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins
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