title: A Serum-Resistant Low-Generation Polyamidoamine with PEI 423 Outer Layer for Gene Delivery Vector.
abstract: A new derivative of polyamidoamine and polyethylenimine, G2.5-PEI 423 or G1.5-PEI 423, is prepared by an amidation reaction of PAMAM G2.5 or PAMAM G1.5 using PEI 423. The polycations show a great ability to combine with pDNA to form complexes, which protect the pDNA from nuclease degradation. The polymers display stronger buffer capacity and lower cytotoxicity. The complexes have particle sizes of 120-180 nm and zeta potentials of 20-40 mV. The G2.5-PEI 423 complexes display much higher transfection efficiencies than PAMAM G5 and Lipo-2k, and the G1.5-PEI 423 complexes display higher transfection efficiencies than PAMAM G4 and PEI-25k. The complexes possess better serum-resistant capacity. The G2.5-PEI 423 has a great potential to be used as a serum-resistant gene vector.
authors: The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2 Road, Guangzhou 510080, P. R. China. gzpshr@163.com
authors: Shirong
authors: S
authors: Pan
authors: Duanwen
authors: D
authors: Cao
authors: Huan
authors: H
authors: Huang
authors: Wu
authors: W
authors: Yi
authors: Linhao
authors: L
authors: Qin
authors: Min
authors: M
authors: Feng
mesh_terms: D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D057809:HEK293 Cells; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008958:Models, Molecular; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D050091:Dendrimers; D046911:Macromolecular Substances; C577210:PAMAM G2.5 polyamidoamine; C531249:Poly(amidoamine); D011073:Polyamines; D013778:Tetrazolium Salts; D013844:Thiazoles; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C022616:thiazolyl blue