title: Comparative antiadhesive properties of crude extract and phenolic fraction isolated from aerial parts of Tribulus pterocarpus during severe hyperhomocysteinemia.
abstract: The phenolic fraction and the crude extract from Tribulus pterocarpus have different biological activity, including antiplatelet-antiadhesive properties. Since it is demonstrated that hyperhomocysteinemia may act as stimulator of blood platelet activation (platelet adhesion, aggregation, and secretion), but various antiplatelet compounds are able to reduce hyperactivation of blood platelets induced by hyperhomocysteinemia. The aim of our present experiments was to investigate in vitro one of the step in platelet activation process - platelet adhesion to collagen induced by the model of severe hyperhomocyateinemia in the presence of the phenolic fraction and the crude extract from T. pterocarpus. Severe hyperhomocysteinemia was induced by reduced form of Hcy in the concentrations 0.1mM and 1mM, or using HTL in the concentrations 0.1, 0.5 and 1 μM. Adhesion of blood platelets to collagen was determined according to Tuszynski and Murphy. We observed that the phenolic fraction and the crude extract from T. pterocarpus have the inhibitory effect on platelet adhesion during severe hyperhomocysteinemia. The action of tested phenolic and crude extract was concentration-dependent, but the phenolic fraction was stronger antiadhesive action than the crude extract. We suggest that T. pterocarpus may be good source of antiplatelet compounds during hyperhomocysteinemia.
authors: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/3, 90-236 Lodz, Poland.
authors: Malgorzata
authors: M
authors: Tomczynska
authors: Joanna
authors: J
authors: Malinowska
authors: Agnieszka
authors: A
authors: Morel
authors: Arafa I
authors: AI
authors: Hamed
authors: Wieslaw
authors: W
authors: Oleszek
authors: Anna
authors: A
authors: Stochmal
authors: Beata
authors: B
authors: Olas
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000187:drug effects*; D006801:Humans; D020138:Hyperhomocysteinemia / Q000188:drug therapy*; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects; D032444:Tribulus / Q000737:chemistry*; D055815:Young Adult
chemical_list: D010636:Phenols; D010936:Plant Extracts