title: Germline allele-specific expression of DAPK1 in chronic lymphocytic leukemia.
abstract: We previously reported a rare germline variant (c.1-6531) that resulted in allele-specific expression (ASE) of death-associated protein kinase 1 (DAPK1) and predisposition to chronic lymphocytic leukemia (CLL). We investigated a cohort of CLL patients lacking this mutation for the presence of ASE of DAPK1. We developed a novel strategy that combines single-nucleotide primer extension (SNuPE) with MALDI-TOF mass spectrometry, and detected germline DAPK1 ASE in 17 out of 120 (14.2%) CLL patients associated with a trend towards younger age at diagnosis. ASE was absent in 63 healthy controls. Germline cells of CLL patients with ASE showed increased levels of DNA methylation in the promoter region, however, neither genetic nor further epigenetic aberrations could be identified in the DAPK1 5' upstream regulatory region, within distinct exons or in the 3'-UTR. We identified B-lymphoid malignancy related cell line models harboring allelic imbalance and found that allele-specific methylation in DAPK1 is associated with ASE. Our data indicate that ASE at the DAPK1 gene locus is a recurrent event, mediated by epigenetic mechanisms and potentially predisposing to CLL.
authors: Division of Epigenomics and Cancer Risk Factors, German Cancer Research Center, Heidelberg, Germany.
authors: Quan-Xiang
authors: QX
authors: Wei
authors: Rainer
authors: R
authors: Claus
authors: Thomas
authors: T
authors: Hielscher
authors: Daniel
authors: D
authors: Mertens
authors: Aparna
authors: A
authors: Raval
authors: Christopher C
authors: CC
authors: Oakes
authors: Stephan M
authors: SM
authors: Tanner
authors: Albert
authors: A
authors: de la Chapelle
authors: John C
authors: JC
authors: Byrd
authors: Stephan
authors: S
authors: Stilgenbauer
authors: Christoph
authors: C
authors: Plass
mesh_terms: D000367:Age Factors; D000483:Alleles; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015331:Cohort Studies; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D064550:Death-Associated Protein Kinases; D018095:Germ-Line Mutation / Q000235:genetics; D005858:Germany; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D051017:Apoptosis Regulatory Proteins; D017931:DNA Primers; C579096:DAPK1 protein, human; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases
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