title: CD44v6 expression is related to mesenchymal phenotype and poor prognosis             in patients with colorectal cancer.
abstract: CD44 standard isoform (CD44s) is a cancer stem cell marker in many tumors,             and is one of the CD44 isoforms.CD44v6 has been reported to correlate with tumor             progression and poor prognosis in colorectal cancer. However, the relevance of             CD44s and CD44v6 to epithelial-mesenchymal transition (EMT) remains unclear. Immunohistochemistry             was performed to investigate the clinical importance of CD44s and CD44v6 and their             relevance to EMT in 113 patients with stage II/III colorectal cancer treated by             curative resection. The relevance of CD44v6 knockdown to the phenotype of colon             cancer cells was examined using small interfering RNA (siRNA) specific for CD44v6             in vitro. CD44v6 expression showed a significant inverse correlation with E-cadherin             expression (P=0.0007) and a positive correlation with vimentin expression (P=0.0096).             A multivariate analysis showed that high CD44v6 expression was an independent             poor prognostic factor for disease-free survival (P=0.01, HR=3.05) and overall             survival (P=0.025, HR=3.16). The clinical significance and the relevance of CD44s             expression to EMT markers was noted to a lesser extent compared to CD44v6 expression.             The knockdown of CD44v6 decreased vimentin expression, cell invasion and HGF-induced             cell migration, but conferred only a slight effect on E-cadherin expression in             colon cancer cells (HCT116 and LoVo). CD44v6 is related to poor outcome of patients             with colorectal cancer via upregulation of the mesenchymal phenotype.
authors: Department of Gastroenterological Surgery, Graduate School of                     Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
authors: Seiya
authors: S
authors: Saito
authors: Hirohisa
authors: H
authors: Okabe
authors: Masayuki
authors: M
authors: Watanabe
authors: Takatsugu
authors: T
authors: Ishimoto
authors: Masaaki
authors: M
authors: Iwatsuki
authors: Yoshifumi
authors: Y
authors: Baba
authors: Youhei
authors: Y
authors: Tanaka
authors: Junji
authors: J
authors: Kurashige
authors: Yuji
authors: Y
authors: Miyamoto
authors: Hideo
authors: H
authors: Baba
mesh_terms: D000328:Adult; D000368:Aged; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D014746:Vimentin / Q000378:metabolism
chemical_list: C473647:CD44S antigen; C112697:CD44v6 antigen; D015820:Cadherins; D018960:Hyaluronan Receptors; D014746:Vimentin