title: CdTe quantum dots enhance feasibility of EvaGreen-based real-time PCR with decent amplification fidelity.
abstract: Quantitative real-time PCR (qPCR), as an important quantitative technique for nucleic acids, has been widely used in many fields including clinical diagnosis, molecular biology, and cancer research. However, non-specific amplification products are still a frequent problem in qPCR. In this study, we investigated the effects of QDs on real-time amplification based on either SYBR Green I or EvaGreen. It was found that QDs could raise the amplification sensitivity and thus enhance the efficiency using SYBR Green I detection system. In the case of EvaGreen detection systems, addition of QDs also led to a better correlation coefficient than without QDs. EvaGreen-based system gave sharper peaks for melting curves than SYBR Green I. The experiments indicated that the polymerase activity could be partially blocked by QDs at the pre-PCR temperatures, resulting in the improvement of PCR specificity. These results indicated that CdTe QDs could be used as a descent qPCR enhancer. Good amplification fidelity in QDs-facilitated qPCR was also a plus that has not been reported elsewhere.
authors: School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, People's Republic of China.
authors: Fuming
authors: F
authors: Sang
authors: Zhizhou
authors: Z
authors: Zhang
authors: Zhong
authors: Z
authors: Xu
authors: Xiaolei
authors: X
authors: Ju
authors: Hongyuan
authors: H
authors: Wang
authors: Shuanghua
authors: S
authors: Zhang
authors: Changlu
authors: C
authors: Guo
mesh_terms: D052160:Benzothiazoles; D019187:Cadmium Compounds / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D003959:Diamines; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D016014:Linear Models; D009930:Organic Chemicals / Q000737:chemistry; D045663:Quantum Dots; D011804:Quinolines; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D013691:Tellurium / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D019187:Cadmium Compounds; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA; D013691:Tellurium; C028337:cadmium telluride
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