title: Circulating miR-150 and miR-342 in plasma are novel potential biomarkers for acute myeloid leukemia.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small (19-22-nt) single-stranded noncoding RNA molecules whose deregulation of expression can contribute to human disease including the multistep processes of carcinogenesis in human. Circulating miRNAs are emerging biomarkers in many diseases and cancers such as type 2 diabetes, pulmonary disease, colorectal cancer, and gastric cancer among others; however, defining a plasma miRNA signature in acute myeloblastic leukemia (AML) that could serve as a biomarker for diagnosis or in the follow-up has not been done yet.
  
  
  METHODS
  TaqMan miRNA microarray was performed to identify deregulated miRNAs in the plasma of AML patients. Quantitative real-time RT-PCR was used to validate the results. Receiver-operator characteristic (ROC) curve analysis was conducted to evaluate the diagnostic accuracy of the highly and significantly identified deregulated miRNA(s) as potential candidate biomarker(s).
  
  
  RESULTS
  The plasma expression level of let-7d, miR-150, miR-339, and miR-342 was down-regulated whilst that of let-7b, and miR-523 was up-regulated in the AML group at diagnosis compared to healthy controls. ROC curve analyses revealed an AUC (the areas under the ROC curve) of 0.835 (95% CI: 0.7119- 0.9581; P<0.0001) and 0.8125 (95% CI: 0.6796-0.9454; P=0.0005) for miR-150, and miR-342 respectively. Combined ROC analyses using these 2 miRNAs revealed an elevated AUC of 0.86 (95% CI: 0.7819-0.94; P<0.0001) indicating the additive effect in the diagnostic value of these 2 miRNAs. QRT-PCR results showed that the expression level of these two miRs in complete remission AML patients resembled that of healthy controls.
  
  
  CONCLUSIONS
  Our findings indicated that plasma miR-150 and miR-342 are novel important promising biomarkers in the diagnosis of AML. These novel and promising markers warrant validation in larger prospective studies.
authors: Laboratory of Experimental Hematology, Institut Jules Bordet, Université Libre de Bruxelles, 121, Boulevard de Waterloo, Bruxelles 1000, Belgium.
authors: Hussein
authors: H
authors: Fayyad-Kazan
authors: Nizar
authors: N
authors: Bitar
authors: Mehdi
authors: M
authors: Najar
authors: Philippe
authors: P
authors: Lewalle
authors: Mohammad
authors: M
authors: Fayyad-Kazan
authors: Rabih
authors: R
authors: Badran
authors: Eva
authors: E
authors: Hamade
authors: Ahmad
authors: A
authors: Daher
authors: Nader
authors: N
authors: Hussein
authors: Rim
authors: R
authors: ElDirani
authors: Fadwa
authors: F
authors: Berri
authors: Luc
authors: L
authors: Vanhamme
authors: Arsène
authors: A
authors: Burny
authors: Philippe
authors: P
authors: Martiat
authors: Redouane
authors: R
authors: Rouas
authors: Bassam
authors: B
authors: Badran
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D020869:Gene Expression Profiling; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000235:genetics; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C533097:MIR150, human; C546176:MIRN342 microRNA, human
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