title: Genomic and phenotypic profiles of two Brazilian breast cancer cell             lines derived from primary human tumors.
abstract: Breast cancer is the most common type of cancer among women worldwide. Research             using breast cancer cell lines derived from primary tumors may provide valuable             additional knowledge regarding this type of cancer. Therefore, the aim of this             study was to investigate the phenotypic profiles of MACL-1 and MGSO-3, the only             Brazilian breast cancer cell lines available for comparative studies. We evaluated             the presence of hormone receptors, proliferation, differentiation and stem cell             markers, using immunohistochemical staining of the primary tumor, cultured cells             and xenografts implanted in immunodeficient mice. We also investigated the ability             of the cell lines to form colonies and copy number alterations by array comparative             genomic hybridization. Histopathological analysis showed that the invasive primary             tumor from which the MACL-1 cell line was derived, was a luminal A subtype carcinoma,             while the ductal carcinoma in situ (DCIS) that gave rise to the MGSO-3 cell line             was a HER2 subtype tumor, both showing different proliferation levels. The cell             lines and the tumor xenografts in mice preserved their high proliferative potential,             but did not maintain the expression of the other markers assessed. This shift             in expression may be due to the selection of an 'establishment' phenotype in vitro.             Whole-genome DNA evaluation showed a large amount of copy number alterations (CNAs)             in the two cell lines. These findings render MACL-1 and MGSO-3 the first characterized             Brazilian breast cancer cell lines to be potentially used for comparative research.
authors: Department of Biochemistry and Immunology, Federal University                     of Minas Gerais, Belo Horizonte, Brazil.
authors: Natássia C R
authors: NC
authors: Corrêa
authors: Hellen
authors: H
authors: Kuasne
authors: Jerusa A Q A
authors: JA
authors: Faria
authors: Ciça C S
authors: CC
authors: Seixas
authors: Iria G D
authors: IG
authors: Santos
authors: Francine B
authors: FB
authors: Abreu
authors: Suely
authors: S
authors: Nonogaki
authors: Rafael M
authors: RM
authors: Rocha
authors: Gerluza
authors: G
authors: Aparecida Borges Silva
authors: Helenice
authors: H
authors: Gobbi
authors: Silvia R
authors: SR
authors: Rogatto
authors: Alfredo M
authors: AM
authors: Goes
authors: Dawidson A
authors: DA
authors: Gomes
mesh_terms: D000818:Animals; D001938:Brazil; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D055028:Comparative Genomic Hybridization; D005260:Female; D006801:Humans; D051379:Mice; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
references: Mol Cancer Ther. 2006 Nov;5(11):2914-8
references: 17121939
references: Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3983-8
references: 12629218
references: J Mol Histol. 2004 Mar;35(3):255-62
references: 15339045
references: Tumour Biol. 2010 Jun;31(3):189-97
references: 20422346
references: Arch Pathol Lab Med. 2010 Jul;134(7):e48-72
references: 20586616
references: Anticancer Res. 2010 Dec;30(12):4851-60
references: 21187462
references: PLoS One. 2009 Jul 03;4(7):e6146
references: 19582160
references: Cancer Res. 2005 Dec 1;65(23):10783-93
references: 16322224
references: Cancer Res. 2007 Sep 1;67(17):8229-39
references: 17804737
references: Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10869-74
references: 11553815
references: Breast Cancer Res. 2004;6(1):22-30
references: 14680482
references: Ann Oncol. 2009 Aug;20(8):1319-29
references: 19535820
references: Cancer Genet Cytogenet. 1996 Jun;88(2):136-40
references: 8640722
references: Cancer Res. 1990 Nov 15;50(22):7184-9
references: 1977515
references: Oncol Rep. 2010 Sep;24(3):677-85
references: 20664973
references: Clin Cancer Res. 2005 Feb 1;11(3):1119-28
references: 15709179
references: Hum Mol Genet. 2001 Jun 15;10(13):1413-9
references: 11440994
references: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8418-23
references: 12829800
references: Genes Chromosomes Cancer. 1998 Feb;21(2):108-12
references: 9491321
references: Genomics. 2000 Feb 15;64(1):44-50
references: 10708517
references: Nat Rev Cancer. 2007 Sep;7(9):659-72
references: 17721431
references: Oncogene. 2003 Feb 27;22(8):1247-52
references: 12606952
references: Nature. 2000 Aug 17;406(6797):747-52
references: 10963602
references: Breast Cancer Res. 2010;12(5):R68
references: 20813035
references: Oncol Res. 2009;17(10):473-82
references: 19725227
references: Int J Cancer. 1998 Jan 19;75(2):181-6
references: 9462705
references: BMC Cancer. 2010 Jan 14;10:15
references: 20070913
references: Genes Chromosomes Cancer. 1995 Dec;14(4):227-51
references: 8605112
references: Nat Protoc. 2006;1(5):2315-9
references: 17406473
references: Clin Cancer Res. 2001 May;7(5):1358-61
references: 11350906
references: Breast Cancer Res Treat. 2007 Jun;103(2):247-57
references: 17063277
references: Arch Pathol Lab Med. 2007;131(1):18-43
references: 19548375
references: Breast Cancer Res. 2008;10(1):R10
references: 18241344
references: Int J Oncol. 2006 Oct;29(4):935-45
references: 16964389
references: Lancet Oncol. 2010 Feb;11(2):174-83
references: 20152769
references: Am J Surg Pathol. 2011 Oct;35(10):e47-63
references: 21934399
references: Biomed Pharmacother. 2005 Oct;59 Suppl 2:S351-4
references: 16507407