title: Analysis of interactions between proteins and fatty acids or cholesterol using a fatty acid/cholesterol pull-down assay.
abstract: Specific interactions between the polar head groups of membrane lipids and proteins have been described previously. In contrast, the specificity of the interaction between lipid acyl chains with proteins is less understood. By combining a fatty acid or cholesterol pull-down assay with Western blot analysis or mass spectrometry, we identified transmembrane and cytosolic proteins that bound preferentially to short or long acyl chains or to cholesterol. Thus, this approach allows identification of specific fatty acid-protein or cholesterol-protein interactions.
authors: Department of Molecular Immunology, Institute for Biology III, BIOSS Center for Biological Signalling Studies, Centre for Chronic Immunodeficiency (CCI), Albert Ludwigs University Freiburg and Max Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.
authors: E
authors: E
authors: Beck-García
authors: K
authors: K
authors: Beck-García
authors: A
authors: A
authors: Schlosser
authors: W W
authors: WW
authors: Schamel
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000378:metabolism; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D019169:Jurkat Cells; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008563:Membrane Lipids / Q000032:analysis / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D000075223:Poly-ADP-Ribose Binding Proteins; D011990:Receptors, Transferrin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C489444:BUB3 protein, human; C495270:CTNNB1 protein, human; D018797:Cell Cycle Proteins; D005227:Fatty Acids; C110810:LAT protein, human; D008563:Membrane Lipids; D008565:Membrane Proteins; D028861:Nuclear Pore Complex Proteins; C465451:Nup98 protein, human; D000075223:Poly-ADP-Ribose Binding Proteins; D011990:Receptors, Transferrin; D051176:beta Catenin; D002784:Cholesterol; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D000604:Amino Acyl-tRNA Synthetases