title: Effect of chronic T-2 toxin exposure in rabbit bucks, determination of the No Observed Adverse Effect Level (NOAEL).
abstract: T-2 toxin (T-2) was administered to adult Pannon White (n = 10/group) male rabbits for 65 days, first in a suspension by gavage (0.05, 0.1 or 0.2 mg/animal/day), and secondly mixed into the feed (0.33 and 0.66 mg/kg feed). In the first experiment 0.1 mg T-2 exposure resulted in temporary decrease in feed intake, slower increase in the gonadotropin-releasing hormone (GnRH) induced testosterone synthesis, slight centrolobular infiltration in the liver and a slight hyperplasia of the Leydig cells. In addition to the temporary feed refusal effect, 0.2 mg T-2 caused a temporary decrease in plasma albumin and urea concentrations, lesser glutathione peroxidase (GPx) activity in the seminal plasma, a greater (by 320%) ratio of spermatozoa with cytoplasmic droplets, slower increase in the GnRH-induced testosterone synthesis, centrolobular infiltration in the liver, slightly hyperaemic testes and increased proliferative activity of the Leydig cells. The two smaller doses applied in feed (0.33 and 0.66 mg/kg) did not cause any significant adverse effect, and no feed refusal was observed. According to these results the No Observed Adverse Effect Level (NOAEL) of T-2 for adult rabbit males was found to be <0.1 mg/animal/day (<0.02 mg/kg b.w./day).
authors: Kaposvár University Faculty of Animal Science, Guba S. 40, 7400 Kaposvár, Hungary. kovacs.melinda@ke.hu
authors: Melinda
authors: M
authors: Kovács
authors: Gábor
authors: G
authors: Tornyos
authors: Zsolt
authors: Z
authors: Matics
authors: Miklós
authors: M
authors: Mézes
authors: Krisztián
authors: K
authors: Balogh
authors: Veronika
authors: V
authors: Rajli
authors: Zsófia
authors: Z
authors: Bloch-Bodnár
authors: Miklós
authors: M
authors: Rusvai
authors: Míra
authors: M
authors: Mándoki
authors: Sándor
authors: S
authors: Cseh
mesh_terms: D000818:Animals; D004435:Eating / Q000187:drug effects / Q000502:physiology; D007987:Gonadotropin-Releasing Hormone / Q000097:blood; D006651:Histocytochemistry / Q000662:veterinary; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D011817:Rabbits / Q000378:metabolism*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013605:T-2 Toxin / Q000633:toxicity*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood
chemical_list: D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D013605:T-2 Toxin