title: Ubc9 promotes invasion and metastasis of lung cancer cells.
abstract: Lung cancer is the leading cause of cancer-related mortality worldwide.             The mortality is high mainly due to the lack of known effective screening procedures;             there is a high tendency for early spread and systemic therapies do not cure metastatic             disease. Thus, it is important to investigate the molecular mechanism(s) of lung             cancer development and, specifically, to identify an effective method by which             to inhibit the invasion and metastasis of lung cancer. Ubiquitin-conjugating enzyme             9 (Ubc9), the sole conjugating enzyme for sumoylation, regulates protein function             and plays a key role in tumorigenesis. Whether Ubc9 is involved in the invasion             and metastasis of lung cancer remains unknown. Herein, we report that Ubc9 exhibits             an important role in lung cancer invasion and metastasis. We first investigated             the biological effect of Ubc9 on lung cancer by cloning the Ubc9 gene into a eukaryotic             expression plasmid and stably expressing it in the human small cell lung cancer             cell line NCI-H446 in order to observe any biological changes. We further analyzed             the effect of Ubc9 in an in vivo experiment, injecting NCI-H446 cells stably overexpressing             Ubc9 into nude mice and analyzing their metastatic ability. Our results demonstrated             that Ubc9 is expressed at higher levels in primary lung cancer tissue and metastatic             nodules as compared to premalignant and/or normal tissue. Furthermore, we demonstrated             that upregulation of Ubc9 expression promotes migration and invasion. Ubc9 likely             plays an important role in cancer progression by promoting invasion and metastasis             in lung cancer.
authors: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, PR China.
authors: Hui
authors: H
authors: Li
authors: Huiyan
authors: H
authors: Niu
authors: Yang
authors: Y
authors: Peng
authors: Jiahe
authors: J
authors: Wang
authors: Ping
authors: P
authors: He
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology; D055752:Small Cell Lung Carcinoma / Q000235:genetics* / Q000473:pathology; D058207:Sumoylation / Q000235:genetics; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000378:metabolism; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D044763:Ubiquitin-Conjugating Enzymes; D000098843:Ubiquitin-Conjugating Enzyme UBC9