title: Increased expression of small heat shock protein αB-crystallin after intracerebral hemorrhage in adult rats.
abstract: αB-crystallin (αBC) is involved in diverse cellular activities. Previous studies demonstrated that αBC had anti-apoptotic and proliferation-promoting effects in multiple diseases. Here, we explored the αBC's roles in the pathophysiology of intracerebral hemorrhage (ICH). An ICH rat model was established and assessed by behavioral tests. Using Western blot and immunohistochemistry, significant up-regulation of αBC was found in neurons and astrocytes in brain areas surrounding the hematoma following ICH. Increase of αBC expression was found to be accompanied by the increased expression of proliferating cell nuclear antigen (PCNA), p53, Bax, and active-caspase-3. αBC was co-localized with PCNA in astrocytes or active-caspase-3 in neurons, suggesting its role in astrocyte proliferation and neuronal apoptosis. Our in vitro study, using αBC RNA interference in PC12 cells, indicated that αBC might exert its anti-apoptotic function in neuronal apoptosis. Thus, αBC may play a role in protecting the brain from secondary damage following ICH.
authors: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors: Kaifu
authors: K
authors: Ke
authors: Lei
authors: L
authors: Li
authors: Ying
authors: Y
authors: Rui
authors: Heyi
authors: H
authors: Zheng
authors: Xiang
authors: X
authors: Tan
authors: Wei
authors: W
authors: Xu
authors: Jianhua
authors: J
authors: Cao
authors: Jian
authors: J
authors: Xu
authors: Gang
authors: G
authors: Cui
authors: Guangfei
authors: G
authors: Xu
authors: Maohong
authors: M
authors: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism / Q000502:physiology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002543:Cerebral Hemorrhage / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D016716:PC12 Cells; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D038203:alpha-Crystallin B Chain / Q000235:genetics / Q000378:metabolism*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D038203:alpha-Crystallin B Chain; D053148:Caspase 3
references: J Mol Histol. 2012 Oct;43(5):527-33
references: 22484641
references: Biochem Biophys Res Commun. 2007 Mar 2;354(1):109-14
references: 17222797
references: Curr Mol Med. 2012 Feb;12(2):177-87
references: 22280356
references: J Cereb Blood Flow Metab. 2000 Feb;20(2):396-404
references: 10698078
references: Neuropathol Appl Neurobiol. 2006 Apr;32(2):119-30
references: 16599941
references: J Stroke Cerebrovasc Dis. 2003 May-Jun;12(3):152-9
references: 17903920
references: Antioxid Redox Signal. 2005 Mar-Apr;7(3-4):404-13
references: 15706087
references: J Cereb Blood Flow Metab. 2007 May;27(5):894-908
references: 17033693
references: Int J Biochem Cell Biol. 2012 Oct;44(10):1622-31
references: 22521623
references: Curr Neurovasc Res. 2008 May;5(2):143-52
references: 18473830
references: Brain Res Bull. 2005 Oct 15;67(3):235-41
references: 16144660
references: Neurocrit Care. 2004;1(1):5-18
references: 16174894
references: Brain Res Bull. 1999 Aug;49(6):377-91
references: 10483914
references: Biochim Biophys Acta. 2011 Aug;1813(8):1532-42
references: 21640763
references: Stroke. 2008 Jul;39(7):2079-84
references: 18436875
references: Brain Res. 2001 Jun 1;902(2):178-89
references: 11384611
references: Toxicol Pathol. 2007 Jun;35(4):495-516
references: 17562483
references: Neurosci Res. 2009 Aug;64(4):355-61
references: 19379782
references: Neurosurgery. 2001 Apr;48(4):875-82; discussion 882-3
references: 11322448
references: Semin Cell Dev Biol. 2000 Feb;11(1):53-60
references: 10736264
references: Acta Neuropathol. 1992;83(3):324-7
references: 1373027
references: J Cereb Blood Flow Metab. 2004 Feb;24(2):159-66
references: 14747742
references: Biochem Biophys Res Commun. 2005 Jun 10;331(3):761-77
references: 15865932
references: J Clin Invest. 2006 Jan;116(1):30-2
references: 16395401
references: Stroke. 2011 Jun;42(6):1781-6
references: 21527759
references: J Immunol. 1995 Jan 1;154(1):363-74
references: 7995955
references: Cell Death Differ. 1999 Feb;6(2):99-104
references: 10200555
references: Cell. 1989 Apr 7;57(1):71-8
references: 2539261
references: Curr Atheroscler Rep. 2012 Aug;14(4):300-6
references: 22538431
references: J Biol Chem. 2001 May 11;276(19):16059-63
references: 11274139
references: J Neurosci. 1999 Sep 15;19(18):7860-9
references: 10479688
references: Dev Cell. 2011 Jul 19;21(1):92-101
references: 21763611
references: J Neurotrauma. 2012 Dec 10;29(18):2798-804
references: 22862734
references: J Biol Chem. 2010 Nov 26;285(48):37324-32
references: 20841355
references: Stroke. 2002 Oct;33(10):2478-84
references: 12364741
references: J Mol Neurosci. 2012 Jun;47(2):357-67
references: 22569987
references: J Cell Sci. 1997 Nov;110 ( Pt 21):2759-69
references: 9427392
references: Cell Death Differ. 2004 May;11(5):512-26
references: 14752512
references: Acta Neuropathol. 2010 Jan;119(1):37-53
references: 19847447
references: J Clin Invest. 2006 Jan;116(1):261-70
references: 16395408
references: J Neuropathol Exp Neurol. 1970 Apr;29(2):254-65
references: 5435822