title: Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: role of Sp1 and CREB.
abstract: Acyl-CoA synthetase 4 (Acsl4) is involved in several cellular functions including steroidogenesis, synaptic development and cancer metastasis. Although the expression of Acsl4 seems to be regulated by tissue- and cell-specific factors as well as pituitary hormones and growth factors, the transcriptional mechanisms involved remain unknown. We demonstrated hCG and cAMP regulation of Acsl4 mRNA in mouse steroidogenic MA-10 Leydig cells. We characterized the transcription initiation site and promoter of the Acsl4 mouse gene and identified three alternative splice variants present in MA-10 cells. Sequence analysis of a 1.5-kb fragment of the Acsl4 promoter revealed the absence of a TATA box and the presence of many putative binding sites for transcription factors including Sp1 and CREB. Functional characterization revealed that the specificity protein/Krüppel-like factor Sp1 binding site in the proximal promoter is involved in basal activity and that the cAMP response element-binding site is involved in cAMP stimulation of Acsl4 transcription.
authors: Institute of Biomedical Investigations (INBIOMED), Department of Biochemistry, School of Medicine, University of Buenos Aires, National Research Council, Buenos Aires, Argentina.
authors: Ulises
authors: U
authors: Orlando
authors: Mariana
authors: M
authors: Cooke
authors: Fabiana
authors: F
authors: Cornejo Maciel
authors: Vassilios
authors: V
authors: Papadopoulos
authors: Ernesto J
authors: EJ
authors: Podestá
authors: Paula
authors: P
authors: Maloberti
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D006063:Chorionic Gonadotropin; D003066:Coenzyme A Ligases; D000242:Cyclic AMP; D017362:Cyclic AMP Response Element-Binding Protein; D012333:RNA, Messenger; D016329:Sp1 Transcription Factor; C492004:Creb1 protein, mouse; C581020:Acsl4 protein, mouse