title: Prolactin regulates transcription of the ion uptake Na+/Cl- cotransporter (ncc) gene in zebrafish gill.
abstract: Prolactin (PRL) is a well-known regulator of ion and water transport within osmoregulatory tissues across vertebrate species, yet how PRL acts on some of its target tissues remains poorly understood. Using zebrafish as a model, we show that ionocytes in the gill directly respond to systemic PRL to regulate mechanisms of ion uptake. Ion-poor conditions led to increases in the expression of PRL receptor (prlra), Na(+)/Cl(-) cotransporter (ncc; slc12a10.2), Na(+)/H(+) exchanger (nhe3b; slc9a3.2), and epithelial Ca(2+) channel (ecac; trpv6) transcripts within the gill. Intraperitoneal injection of ovine PRL (oPRL) increased ncc and prlra transcripts, but did not affect nhe3b or ecac. Consistent with direct PRL action in the gill, addition of oPRL to cultured gill filaments stimulated ncc in a concentration-dependent manner, an effect blocked by a pure human PRL receptor antagonist (Δ1-9-G129R-hPRL). These results suggest that PRL signaling through PRL receptors in the gill regulates the expression of ncc, thereby linking this pituitary hormone with an effector of Cl(-) uptake in zebrafish for the first time.
authors: Department of Biology, Center for Neuroendocrine Studies, University of Massachusetts, Amherst, MA 01003, USA.
authors: Jason P
authors: JP
authors: Breves
authors: Sandy B
authors: SB
authors: Serizier
authors: Vincent
authors: V
authors: Goffin
authors: Stephen D
authors: SD
authors: McCormick
authors: Rolf O
authors: RO
authors: Karlstrom
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D005880:Gills / Q000187:drug effects / Q000378:metabolism*; D017136:Ion Transport; D011388:Prolactin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology*; D011981:Receptors, Prolactin / Q000037:antagonists & inhibitors / Q000378:metabolism; D049992:Sodium Chloride Symporters / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014867:Water / Q000737:chemistry; D014882:Water-Electrolyte Balance / Q000187:drug effects; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011981:Receptors, Prolactin; D049992:Sodium Chloride Symporters; D029961:Zebrafish Proteins; C506484:prolactin, delta(1-9)-Arg(129)-; D014867:Water; D011388:Prolactin
references: Gen Comp Endocrinol. 2007 Oct-Dec;154(1-3):31-40
references: 17714712
references: J Biol Chem. 2003 Sep 19;278(38):35988-99
references: 12824168
references: Am J Physiol Regul Integr Comp Physiol. 2009 Mar;296(3):R549-57
references: 19073903
references: Comp Biochem Physiol A Mol Integr Physiol. 2011 Feb;158(2):194-200
references: 21056111
references: Acta Physiol (Oxf). 2011 Jul;202(3):349-59
references: 20874801
references: J Endocrinol. 2007 Aug;194(2):373-92
references: 17641286
references: Birth Defects Res C Embryo Today. 2011 Sep;93(3):205-14
references: 21932430
references: Gen Comp Endocrinol. 2006 May 15;147(1):3-8
references: 16457828
references: Annu Rev Physiol. 2011;73:183-211
references: 21314433
references: Am J Physiol Regul Integr Comp Physiol. 2003 Dec;285(6):R1305-16
references: 12893656
references: PLoS One. 2011;6(8):e23689
references: 21887296
references: Gen Comp Endocrinol. 1990 Mar;77(3):368-77
references: 2338217
references: J Endocrinol. 2007 Jun;193(3):481-91
references: 17535885
references: Mol Cell Endocrinol. 2011 Apr 30;337(1-2):114-21
references: 21345362
references: Life Sci. 2000;66(7):593-605
references: 10794515
references: Am J Physiol Regul Integr Comp Physiol. 2009 Jun;296(6):R1897-906
references: 19386990
references: FEBS Lett. 1997 Mar 17;405(1):91-4
references: 9094431
references: Gen Comp Endocrinol. 2001 Jan;121(1):32-47
references: 11161768
references: Am J Physiol Regul Integr Comp Physiol. 2005 Oct;289(4):R1202-11
references: 15947067
references: Comp Biochem Physiol A Mol Integr Physiol. 2013 Jan;164(1):249-57
references: 23010242
references: BMC Mol Biol. 2008 Nov 12;9:102
references: 19014500
references: Am J Physiol Regul Integr Comp Physiol. 2010 Aug;299(2):R702-10
references: 20504910
references: Prog Clin Biol Res. 1986;205:53-74
references: 3513197
references: PLoS One. 2009 Jul 08;4(7):e6163
references: 19584915
references: Mol Cell Endocrinol. 2012 Nov 25;364(1-2):113-25
references: 22963886
references: J Exp Biol. 2009 Jun;212(Pt 11):1745-52
references: 19448083
references: Gen Comp Endocrinol. 2001 Jun;122(3):270-86
references: 11356039
references: Gen Comp Endocrinol. 2012 Jan 15;175(2):244-50
references: 22137911
references: Am J Physiol Cell Physiol. 2009 Oct;297(4):C845-54
references: 19657057
references: Mol Endocrinol. 2006 Apr;20(4):871-80
references: 16339273
references: Gen Comp Endocrinol. 2007 Jun-Jul;152(2-3):206-14
references: 17418841
references: J Mol Evol. 2007 Oct;65(4):359-72
references: 17917757
references: Dev Biol. 2003 Feb 1;254(1):19-35
references: 12606279
references: Endocr Rev. 1998 Jun;19(3):225-68
references: 9626554
references: Physiol Genomics. 2007 Jun 19;30(1):53-60
references: 17299134
references: Acta Biochim Biophys Sin (Shanghai). 2007 May;39(5):384-90
references: 17492136
references: Cell Mol Life Sci. 2011 Aug;68(16):2797-809
references: 21104292
references: Physiol Rev. 2000 Oct;80(4):1523-631
references: 11015620
references: J Endocrinol. 2006 Jun;189(3):425-39
references: 16731775
references: Comp Biochem Physiol B Biochem Mol Biol. 2005 Feb;140(2):211-8
references: 15649768
references: Am J Physiol. 1989 Mar;256(3 Pt 2):R707-15
references: 2538086
references: Gen Comp Endocrinol. 2006 May 15;147(1):24-30
references: 16406056
references: Gen Comp Endocrinol. 2006 May 1;146(3):318-28
references: 16430892
references: J Endocrinol. 2007 Aug;194(2):417-27
references: 17641289
references: Gen Comp Endocrinol. 1984 Oct;56(1):19-23
references: 6092210
references: Gen Comp Endocrinol. 1991 Aug;83(2):283-9
references: 1655557
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Am J Physiol Regul Integr Comp Physiol. 2007 Jan;292(1):R470-80
references: 16946087
references: Am J Physiol Cell Physiol. 2007 Dec;293(6):C1814-23
references: 17913841
references: FEBS J. 2007 Nov;274(22):5790-8
references: 17944942
references: J Exp Biol. 2009 Jul;212(Pt 13):2007-15
references: 19525426
references: Am J Physiol Regul Integr Comp Physiol. 2009 May;296(5):R1650-60
references: 19279294
references: Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6037-41
references: 7597076
references: J Exp Biol. 2008 Aug;211(Pt 16):2584-99
references: 18689412
references: Genes Dev. 1999 Feb 15;13(4):388-93
references: 10049354
references: Biochim Biophys Acta. 2003 Dec 30;1618(2):207-18
references: 14729157
references: BMC Genomics. 2007 Oct 04;8:354
references: 17915033
references: J Exp Biol. 2009 Oct;212(Pt 20):3283-95
references: 19801433