title: Contact repulsion controls the dispersion and final distribution of Cajal-Retzius cells.
abstract: Cajal-Retzius (CR) cells play a fundamental role in the development of the mammalian cerebral cortex. They control the formation of cortical layers by regulating the migration of pyramidal cells through the release of Reelin. The function of CR cells critically depends on their regular distribution throughout the surface of the cortex, but little is known about the events controlling this phenomenon. Using time-lapse video microscopy in vivo and in vitro, we found that movement of CR cells is regulated by repulsive interactions, which leads to their random dispersion throughout the cortical surface. Mathematical modeling reveals that contact repulsion is both necessary and sufficient for this process, which demonstrates that complex neuronal assemblies may emerge during development through stochastic events. At the molecular level, we found that contact repulsion is mediated by Eph/ephrin interactions. Our observations reveal a mechanism that controls the even distribution of neurons in the developing brain.
authors: Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas & Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain.
authors: Verona
authors: V
authors: Villar-Cerviño
authors: Manuel
authors: M
authors: Molano-Mazón
authors: Timothy
authors: T
authors: Catchpole
authors: Miguel
authors: M
authors: Valdeolmillos
authors: Mark
authors: M
authors: Henkemeyer
authors: Luis M
authors: LM
authors: Martínez
authors: Víctor
authors: V
authors: Borrell
authors: Oscar
authors: O
authors: Marín
mesh_terms: D000367:Age Factors; D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D064032:Calbindin 2; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D009924:Organ Culture Techniques; D036225:Receptor, EphB1 / Q000235:genetics; D036183:Receptor, EphB2 / Q000235:genetics; D036223:Receptor, EphB3 / Q000235:genetics; D000091042:Reelin Protein; D064030:S100 Calcium Binding Protein G / Q000235:genetics
chemical_list: D064032:Calbindin 2; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D064030:S100 Calcium Binding Protein G; D049452:Green Fluorescent Proteins; D036225:Receptor, EphB1; D036183:Receptor, EphB2; D036223:Receptor, EphB3; C000717128:Reln protein, mouse
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