title: Cerium oxide-graphene as the matrix for cholesterol sensor.
abstract: A simple and sensitive electrogenerated chemiluminescence (ECL) cholesterol biosensor was prepared based on cerium oxide-graphene (CeO(2)-graphene) composites as an efficient matrix. CeO(2)-graphene composites were prepared by depositing CeO(2) onto graphene and were characterized by scanning electron microscopy. The experimental results demonstrated that CeO(2)-graphene could catalyze the ECL of a luminol-H(2)O(2) (hydrogen peroxide) system to amplify the luminol ECL signal greatly. In addition, the use of CeO(2)-graphene provided a better biocompatible microenvironment for the immobilized enzyme, resulting in excellent stability and a long lifetime of the ECL biosensor. The surface assembly process, ECL behaviors, and electrochemistry of the biosensor were investigated in detail. The quantity of cholesterol was in the linear range from 12 μM to 7.2 mM with a detection limit of 4.0 μM (signal/noise = 3). In addition, the biosensor exhibited outstanding reproducibility, long-term stability, and selectivity. Moreover, this cholesterol biosensor offers an alternative analytical method with low cost and high speed.
authors: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors: Meihe
authors: M
authors: Zhang
authors: Ruo
authors: R
authors: Yuan
authors: Yaqin
authors: Y
authors: Chai
authors: Cun
authors: C
authors: Wang
authors: Xiaoping
authors: X
authors: Wu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002563:Cerium / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis*; D004800:Enzymes, Immobilized; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D002563:Cerium; D008165:Luminol; C030583:ceric oxide; D006108:Graphite; D002784:Cholesterol