title: [Chemical constituents contained in fatty oil from seeds of Cucumis sativus].
abstract: OBJECTIVE
  To study the chemical constituents contained in the seeds of Cucumis sativus.
  
  
  METHODS
  The fatty oil was extracted by heating and refluxing with petroleum ether. Potassium hydroxide-methanol solution was used for saponification. An unsaponifiable matter was extracted by EtOAc and separated with various chromatographic methods. Its structure was identified on the basis of their physicochemical properties and spectral data. The fatty acid fraction was methyl-esterified and determined by GC. The composition and relative content of fatty acid were determined with normalization method of peak area.
  
  
  RESULTS
  24-ethylcholesta-7, 22, 25-trienol (1), 24-ethylcholesta-7, 25-dienol (2) ,avenasterol (3), spinasterol (4), karounidiol (5) and isokarounidiol (6) were separated and identified from the unsaponifiable matter. Myristic acid (7, 0.12%), palmitic acid (8, 12.04%), palmitoleic acid (9, 0.09%), heptadecanoic acid (10, 0.06%), stearic acid (11, 5.64%), oleic acid (12, 6.95%), linoleic acid (13, 74.40%), arachidic acid (14, 0.19%), and alpha-linolenic acid (15, 0.51%) were identified from the fatty acids part.
  
  
  CONCLUSIONS
  Compounds 5, 6, 9, 10, 14,and 15 were first reported in C. sativus.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. wuxiaoyi0000@126.com
authors: Xiao-Yi
authors: XY
authors: Wu
authors: Zhi-Mao
authors: ZM
authors: Chao
authors: Chun
authors: C
authors: Wang
authors: Zhi-Gao
authors: ZG
authors: Tan
authors: Wen
authors: W
authors: Sun
mesh_terms: D018553:Cucumis sativus / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D012639:Seeds / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D010938:Plant Oils