title: Evaluation of the QMS® Teicoplanin Immunoassay (ThermoFisher Scientific) on Cobas® 8000 System (Roche Diagnostics) and comparison to fluorescence polarization immunoassay for the determination of teicoplanin concentrations in human plasma.
abstract: BACKGROUND
  The performances of the QMS(®) Teicoplanin immunoassay recently developed on Cobas(®) 6000/8000 systems were evaluated and compared to a fluorescence polarization immunoassay (FPIA) [Teicoplanin Innofluor(®) Assay (Thermo Fisher Scientific, Indianapolis, IN)] on FLX analyzer (Abbott Laboratories, Abbott Park, IL)].
  
  
  METHODS
  The validation was performed according to the Cofrac (French Accreditation Committee) document SH GTA 04. For the comparison, 48 plasma samples were analyzed by FPIA and QMS assays.
  
  
  RESULTS
  The QMS assay is accurate (intra assay and inter assay inaccuracy ≤ 2.4%) and precise (intra assay and inter assay imprecision ≤ 10.2%). A linear relationship [QMS = 1.0319 × FPIA - 2.8518, r(2) = 0.9246 (P < 0.001)] between FPIA and QMS was found. In the Bland-Altman plots, no systematic bias was found even if QMS results trends to be lower (mean of the ratio QMS concentration/FPIA concentration = 0.91).
  
  
  CONCLUSIONS
  These results between QMS and FPIA are consistent, which indicates that QMS(®) Teicoplanin immunoassay on Cobas(®) 8000 System is an alternative to FPIA.
authors: Clinical Pharmacology Department, Hôtel Dieu, CHU de Nantes, France. eric.dailly@chu-nantes.fr
authors: Eric
authors: E
authors: Dailly
authors: François
authors: F
authors: Fraissinet
authors: Guillaume
authors: G
authors: Deslandes
authors: Régis
authors: R
authors: Bouquié
authors: Pascale
authors: P
authors: Jolliet
mesh_terms: D016903:Drug Monitoring / Q000379:methods* / Q000592:standards*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D016014:Linear Models; D015203:Reproducibility of Results; D017334:Teicoplanin / Q000097:blood*
chemical_list: D017334:Teicoplanin
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