title: [Transplantation of human umbilical cord mesenchymal stem cells alleviates the progression of FSGS in rats].
abstract: OBJECTIVE
  To study the protective effect of human umbilical cord mesenchymal stem cells (HuMSCs) on the progression of focal segmental glomerulosclerosis (FSGS) in rats.
  
  
  METHODS
  HuMSCs were incubated by adherent culture. Sprague Dawley rats were randomly assigned to 3 groups: No intervention was applied to the rats of normal control group. Model group and transplant group were injected with adriamycin 4 mg/kg (on day 1) and 3.5 mg/kg (on day 8) via the tail vein, in addition, the transplant group rats were injected with 1 mL HuMSCs suspension (2×10(6);/mL) via the tail vein on day 1, 8, 15 and 22, whereas the model group rats were injected with the same dosage of DMEM in the same way.
  
  
  RESULTS
  Transplantation of HuMSCs remarkably alleviated urine protein excretion and hypoalbuminemia, decreased serum cystatin C level and improved kidney damage and inflammatory cell infiltration in renal interstitium. Furthermore, HuMSCs decreased the expressions of TGF-β1 and connective tissue growth factor (CTGF) mRNA in kidney and reduced the levels of serum IL-6 and TNF-α.
  
  
  CONCLUSIONS
  Transplantation of HuMSCs could alleviate or inhibit the progression of FSGS in rats, of which the mechanism might be related to the regulation the synthesis and release of inflammatory mediators and the inhibition of inflammatory cell infiltration.
authors: Department of Nephrology, Tsinghua University, Shenzhen, China. mahualin0796@sina.com
authors: Hualin
authors: H
authors: Ma
authors: Wanfan
authors: W
authors: Zhang
authors: Yong
authors: Y
authors: Dai
authors: Furong
authors: F
authors: Li
authors: Yaojiong
authors: Y
authors: Wu
authors: Xinzhou
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D055513:Connective Tissue Growth Factor / Q000097:blood / Q000235:genetics; D018450:Disease Progression; D005923:Glomerulosclerosis, Focal Segmental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor