title: [Study on the role of fluorescence in situ hybridization in cytogenetic evaluation of myelodysplastic syndrome].
abstract: OBJECTIVE
  To exploit the role of bone marrow (BM) and peripheral blood (PB) fluorescence in situ hybridization (FISH) in cytogenetic evaluation of myelodysplastic syndrome (MDS).
  
  
  METHODS
  The metaphase cytogenetics and BM interphase FISH were prospectively compared in 112 cases of de novo MDS. At the same time, comparison of BM and PB FISH was conducted in 56 cases.
  
  
  RESULTS
  The differences between metaphase cytogenetics and BM FISH were observed in 22 (54%) of 41 cases with clonal karyotypic abnormalities, most of differences were caused by the limitation of FISH probe panel which could not target all of the regions with aberrations. Only 6 (27%) of 22 differences were involved in our probe regions, the FISH results did not change their cytogenetic risk categories. BM FISH testing was abnormal in 15 (21%) of 71 cases with normal karyotypes, FISH testing was abnormal in 14/51 (27%) and 1/20 (5%) cases with fewer than 20 normal metaphases or more than 20 normal metaphases. Comparison of FISH results of PB and BM samples showed abnormal PB FISH results in 21 (72%) of 29 cases with abnormal BM FISH results, and in 1 (4%) of 27 cases with normal BM FISH results.
  
  
  CONCLUSIONS
  BM FISH should be used to MDS cases with fewer than 20 normal metaphases. Although PB FISH testing is limited by a relatively high false negative rate, it is a reasonable choice to cases with failure of BM aspiration.
authors: Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin, China.
authors: Shi-qiang
authors: SQ
authors: Qu
authors: Ze-feng
authors: ZF
authors: Xu
authors: Cheng-wen
authors: CW
authors: Li
authors: Xu-ping
authors: XP
authors: Liu
authors: Tie-jun
authors: TJ
authors: Qin
authors: Yue
authors: Y
authors: Zhang
authors: Zhi-jian
authors: ZJ
authors: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000235:genetics*; D011446:Prospective Studies; D055815:Young Adult