title: The first investigation of west nile virus in horses using real time rt-PCR in middle black sea region in Turkey.
abstract: BACKGROUND
  West Nile Virus (WNV) is a mosquito-borne disease that can cause fatal infection in mammals including humans, dogs, horses, birds and reptiles. Although West Nile Virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. The aim of this study was to the investigate virological presence of WNV in horses in the Black Sea Region of Turkey using real time RT-PCR (rRT-PCR).
  
  
  METHODS
  Totally, 120 horse sera were collected equally from 4 provinces in Middle Black Sea Region of Turkey and investigated for WNV presence by Taqman based rRT-PCR.
  
  
  RESULTS
  WNV nucleic acid was not detected in any horse serum sample.
  
  
  CONCLUSIONS
  Although obtained result indicated no evidence of WNV-RNA in horses, Black Sea Region of Turkey is one of the suitable places for the WNV infection. For this reason, our research will continue for the determination of the viruses in vectors and susceptible animals such as horses, dogs, etc.
authors: Department of Virology, Faculty of Veterinary Medicine,Ondokuz Mayis University, Samsun, Turkey.
authors: Zafer
authors: Z
authors: Yazici
authors: Harun
authors: H
authors: Albayrak
authors: Emre
authors: E
authors: Ozan
authors: Semra
authors: S
authors: Gumusova
keywords: Horses; RT-PCR; Sera; West Nile Virus
references: Vector Borne Zoonotic Dis. 2004 Spring;4(1):71-82
references: 15018775
references: Eur J Clin Microbiol Infect Dis. 2004 Mar;23(3):147-56
references: 14986160
references: Emerg Infect Dis. 2004 Jul;10(7):1314-7
references: 15324558
references: Emerg Infect Dis. 2002 Apr;8(4):380-6
references: 11971771
references: Vet Res. 2004 Jul-Aug;35(4):467-83
references: 15236677
references: Am J Trop Med Hyg. 1999 Oct;61(4):600-11
references: 10548295
references: Emerg Infect Dis. 2000 Jul-Aug;6(4):319-28
references: 10905964
references: Am J Trop Med Hyg. 1993 Mar;48(3):440-6
references: 8470779
references: Curr Opin Infect Dis. 2001 Oct;14(5):519-25
references: 11964871
references: Emerg Infect Dis. 2005 Aug;11(8):1167-73
references: 16102302
references: J Vector Ecol. 2007 Dec;32(2):202-6
references: 18260509
references: Emerg Infect Dis. 2001 Jul-Aug;7(4):611-4
references: 11585520
references: Emerg Infect Dis. 2001 Jul-Aug;7(4):692-6
references: 11585534
references: Mikrobiyol Bul. 1977 Jan;11(1):3-17
references: 857119
references: Turk Hij Tecr Biyol Derg. 1972;32(2):134-43
references: 4668350
references: Vet Pathol. 2001 Jul;38(4):414-21
references: 11467475
references: Emerg Infect Dis. 2001 Jul-Aug;7(4):665-9
references: 11589171
references: Emerg Infect Dis. 1999 Sep-Oct;5(5):643-50
references: 10511520
references: Rev Sci Tech. 2000 Apr;19(1):166-76
references: 11189714
references: J Clin Microbiol. 2000 Nov;38(11):4066-71
references: 11060069
references: Zentralbl Bakteriol Orig A. 1973 Oct;225(1):19-26
references: 4148897
references: Epidemiol Infect. 2006 Aug;134(4):826-9
references: 16316496