title: Infusion of megakaryocytic progenitor products generated from cord blood hematopoietic stem/progenitor cells: results of the phase 1 study.
abstract: BACKGROUND
  Currently, a constant shortage in the supply of platelets has become an important medical and society challenge, especially in developing country, and the in vitro production of megakaryocytic progenitor cells (MPs) from cord blood could represent an effective platelet substitute. In the present study, our objective was to determine the safety and feasibility of ex vivo generated MPs in patients.
  
  
  RESULTS
  MPs were produced and characterized from cord blood mononuclear cells under a serum free medium with cytokines. We investigated the feasibility of expansion and infusion of cord blood-derived MPs in 24 patients with advanced hematological malignancies. The primary end point was the safety and tolerability of the infusion of cord blood-derived MPs. No adverse effects were observed in patients who received ex vivo-generated cells at concentrations of up to a median value of 5.45 × 10(6)cells/kg of body weight. With one year follow-up, acute and chronic GVHD had not been observed among patients who received MPs infusion, even without ABO blood group and HLA typing matching.
  
  
  CONCLUSIONS
  These initial results in patients are very encouraging. They suggest that infusion of cord blood-derived MPs appears safe and feasible for treatment of thrombocytopenia.
authors: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing, China.
authors: Jiafei
authors: J
authors: Xi
authors: Honghu
authors: H
authors: Zhu
authors: Daqing
authors: D
authors: Liu
authors: Xue
authors: X
authors: Nan
authors: Wen
authors: W
authors: Zheng
authors: Kaiyan
authors: K
authors: Liu
authors: Wei
authors: W
authors: Shi
authors: Lin
authors: L
authors: Chen
authors: Yang
authors: Y
authors: Lv
authors: Fang
authors: F
authors: Yan
authors: Yanhua
authors: Y
authors: Li
authors: Xiaoyan
authors: X
authors: Xie
authors: Yunfang
authors: Y
authors: Wang
authors: Wen
authors: W
authors: Yue
authors: Xin
authors: X
authors: Xu
authors: Xiaofei
authors: X
authors: Wei
authors: Jun
authors: J
authors: Zhu
authors: Xiaojun
authors: X
authors: Huang
authors: Xuetao
authors: X
authors: Pei
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006086:Graft vs Host Disease / Q000517:prevention & control*; D019337:Hematologic Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000166:cytology / Q000637:transplantation*; D008875:Middle Aged; D013921:Thrombocytopenia / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D016895:Culture Media, Serum-Free; D016207:Cytokines
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