title: [Mechanism of DNA polymerase beta hyper-expression in malignant transformation induced by benzo[a] pyrene].
abstract: OBJECTIVE
  To explore the mechanism of the hyper-expression of DNA polymerase beta (polbeta) in benzo[a]pyrene (BaP) induced malignant transformed cell (polbeta-T).
  
  
  METHODS
  The mutation of polbeta gene exon and promoter were examined using reverse transcriptase-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) and gene sequencing. The expression of protein-arginine N-methyhransferase 6 (PRMT6) mRNA and protein in polbeta-T cell and control cell (polbeta cell) were investigated by RT-PCR and Western blot.
  
  
  RESULTS
  RT-PCR-SSCP and gene sequencing revealed that the hyper-expression of polbeta in polbeta-T cell was not associated with the mutation of polbeta gene exon while insert mutation (G) and point mutation (C-->A) were found located in the core region of polbeta gene promoter. Furthermore, the expression of PRMT6 mRNA and protein also increased in polbeta-T cell compared with control cell (P<0.05).
  
  
  CONCLUSIONS
  The enhancement of expression of polbeta in polbeta-T cell might be attributed to the mutations locating in polbeta gene promoter on transcription level of polbeta gene, and PRMT6 might also enhance the expression of polbeta in polbeta-T cell through relative epigenetic pathways.
authors: Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors: Wei
authors: W
authors: Zhao
authors: Mei
authors: M
authors: Wu
authors: Mo
authors: M
authors: Yang
authors: Zun-Zhen
authors: ZZ
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D019951:DNA Polymerase beta / Q000235:genetics / Q000378:metabolism*; D005091:Exons; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D001564:Benzo(a)pyrene; C577312:PRMT6 protein, mouse; D011484:Protein-Arginine N-Methyltransferases; D019951:DNA Polymerase beta