title: Wenshen Xiaozheng Tang suppresses the growth of endometriosis with an antiangiogenic effect.
abstract: OBJECTIVE
  To evaluate the effect of Wenshen Xiaozhng Tang (WXT) on ectopic endometrial growth and on angiogenesis in endometrial implants in a rat model.
  
  
  METHODS
  Sprague-Dawley rats with endometriotic implants were randomly treated with low-dose WXT, high-dose WXT, or vehicle (negative control) for 28 days. Cell proliferation and vascular density in the lesions were assessed by immunohistochemistry. The levels of VEGF in peritoneal fluid were determined by ELISA. The mRNA expression of HIF-1α and Flk-1 in the endometriotic lesions was evaluated by real-time PCR.
  
  
  RESULTS
  WXT treatment significantly decreased the lesion size and inhibited cell proliferation in the endometriotic lesions. Lowered vascular density and reduced mRNA expression of HIF-1α in the endometriotic lesions, associated with decreased concentration of VEGF in peritoneal fluid, were also observed in WXT-treated rats.
  
  
  CONCLUSIONS
  These results suggest that WXT could be effective to suppress the growth of endometriosis, partially through its antiangiogenic activity.
authors: * Laboratory of Gynecological Oncology, The Hospital on Integration of Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine , Nanjing, Jiangsu.
authors: Z Z
authors: ZZ
authors: Zhang
authors: C P
authors: CP
authors: Hu
authors: W W
authors: WW
authors: Tang
authors: T
authors: T
authors: Gui
authors: R Y
authors: RY
authors: Qian
authors: Y X
authors: YX
authors: Xing
authors: P
authors: P
authors: Cao
authors: G P
authors: GP
authors: Wan
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004715:Endometriosis / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000517:prevention & control; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics
chemical_list: D020533:Angiogenesis Inhibitors; D004365:Drugs, Chinese Herbal; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C000592912:wenshen xiaozheng tang; D040301:Vascular Endothelial Growth Factor Receptor-2