title: Identification of microRNAs specifically expressed in hepatitis C virus-associated hepatocellular carcinoma.
abstract: Although several studies have investigated the association of miRNAs with hepatocellular carcinoma (HCC), the data published so far are not concordant. A reason for these discrepancies may be the fact that most studies used the nontumorous tissue surrounding the HCC lesion as a control, which is almost invariably affected by cirrhosis or chronic hepatitis, as well as other pathological conditions such as hepatitis B virus (HBV) or hepatitis C virus (HCV) infection. Moreover, HCC is often analyzed as a single group regardless of the different viral etiologies. The miRNAs differentially expressed in HCV-related HCC were investigated by comparing the tumorous tissues to a wide range of liver specimens, including healthy livers obtained from liver donors and patients who underwent liver resection for angioma, in addition to tissues from various acute and chronic liver diseases, including HCV-related cirrhosis not associated with HCC, HCV-related cirrhosis associated with HCC and HBV-associated acute liver failure. The whole set of 2,226 human miRNAs were examined, including 1,121 pre-miRNAs and 1,105 mature miRNAs, available in a microarray platform. Stringent statistical methods were applied to reduce the risk of false discoveries to less than 1%. These data identified 18 miRNAs exclusively expressed in HCV-associated HCC, characterized by high specificity and selectivity versus all other liver diseases and healthy conditions and connected into a regulatory network pivoting on p53, phosphatase and tensin homolog and all-trans retinoic acid signaling.
authors: Department of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
authors: Giacomo
authors: G
authors: Diaz
authors: Marta
authors: M
authors: Melis
authors: Ashley
authors: A
authors: Tice
authors: David E
authors: DE
authors: Kleiner
authors: Lopa
authors: L
authors: Mishra
authors: Fausto
authors: F
authors: Zamboni
authors: Patrizia
authors: P
authors: Farci
mesh_terms: D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000821:virology; D005260:Female; D016174:Hepacivirus / Q000472:pathogenicity*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged
chemical_list: D035683:MicroRNAs
references: Stat Med. 2007 Oct 30;26(24):4428-40
references: 17357994
references: Clin Cancer Res. 2008 Jan 15;14(2):419-27
references: 18223217
references: Swiss Med Wkly. 2011 Oct 24;141:w13287
references: 22020555
references: Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):264-9
references: 20018759
references: J Hepatol. 2012 Aug;57(2):462-6
references: 22504335
references: Gastroenterology. 2010 Jan;138(1):231-40.e1-5
references: 19686745
references: Br J Haematol. 2008 May;141(5):672-5
references: 18318758
references: J Hepatol. 2012 Jan;56(1):167-75
references: 21749846
references: Hepatol Res. 2009 Aug;39(8):786-94
references: 19473441
references: J Hepatol. 2012 Jun;56(6):1371-83
references: 22314424
references: Int J Cancer. 2008 Oct 1;123(7):1616-22
references: 18649363
references: Hepatol Res. 2009 Feb;39(2):177-86
references: 19208038
references: Br J Cancer. 2011 Jan 18;104(2):235-40
references: 21102580
references: FASEB J. 2012 Jul;26(7):3032-41
references: 22459148
references: Oncogene. 2013 Apr 11;32(15):1910-20
references: 22710713
references: Am J Pathol. 2008 Sep;173(3):856-64
references: 18688024
references: Gastroenterology. 2012 Jun;142(7):1431-43
references: 22504185
references: Cancer. 1954 May;7(3):462-503
references: 13160935
references: RNA. 2008 Nov;14(11):2348-60
references: 18812439
references: Proc Natl Acad Sci U S A. 2010 May 11;107(19):8766-71
references: 20421498
references: Cell. 1993 Dec 3;75(5):843-54
references: 8252621
references: Cancer Cell. 2011 Feb 15;19(2):232-43
references: 21316602
references: Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4402-7
references: 19246379
references: Gastroenterology. 2007 Aug;133(2):647-58
references: 17681183
references: Gastroenterology. 2011 May;140(5):1618-28.e16
references: 21324318
references: Oncogene. 2006 Feb 23;25(8):1261-71
references: 16205629
references: Transl Res. 2011 Apr;157(4):241-52
references: 21420035
references: Cancer Res. 1993 Oct 1;53(19):4691-4
references: 8402647
references: Oncogene. 2006 Apr 20;25(17):2537-45
references: 16331254
references: Cancer Res. 2009 Feb 1;69(3):1135-42
references: 19155302
references: Clin Cancer Res. 2010 Feb 1;16(3):867-75
references: 20103675
references: Mol Cell Biol. 2009 Oct;29(19):5290-305
references: 19635812
references: Cancer Res. 2010 Jun 15;70(12):5184-93
references: 20501828
references: Cancer Res. 2007 Jul 1;67(13):6092-9
references: 17616664
references: Science. 2003 Jul 18;301(5631):336-8
references: 12869753
references: Biochem Biophys Res Commun. 2012 May 25;422(1):187-93
references: 22569043
references: Hepatology. 2008 Jun;47(6):1955-63
references: 18433021
references: Mol Carcinog. 2011 Feb;50(2):136-42
references: 21229610
references: Cancer Inform. 2007 Feb 04;3:11-7
references: 19455231
references: Cancer Res. 2011 Jan 1;71(1):78-86
references: 21199797
references: J Biol Chem. 2008 Jun 27;283(26):18158-66
references: 18456660
references: Clin Cancer Res. 2011 Apr 1;17(7):1722-30
references: 21350001
references: Hepatology. 2012 Mar;55(3):821-32
references: 21932399
references: Cancer Res. 2008 Jul 15;68(14):5619-27
references: 18632614