title: Regulation of the proliferation of colon cancer cells by compounds that affect glycolysis, including 3-bromopyruvate, 2-deoxyglucose and biguanides.
abstract: In previous studies performed by our group, we observed that 2-deoxyglucose blocked the acidification of the medium used for culture of colon cancer cells caused by incubation with biguanides and it had an additive inhibitory effect on growth. In the present work, we found that 3-bromopyruvate can also prevent the lowering of pH caused by biguanide treatment. 3-Bromopyruvate inhibited colonic cancer cell proliferation, but the effect was not always additive to that of biguanides and an additive effect was more notable in combined treatment with 3-bromopyruvate and 2-deoxyglucose. The induction of alkaline phosphatase activity by butyrate was not consistently affected by combination with other agents that modified glucose metabolism. The drug combinations that were examined inhibited proliferation of wild-type and p53-null cells and affected colonic cancer lines with different growth rates.
authors: Department of Biochemistry and Molecular Biology, UMDNJ - New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA. lea@umdnj.edu
authors: Michael A
authors: MA
authors: Lea
authors: Mehreen S
authors: MS
authors: Qureshi
authors: Michael
authors: M
authors: Buxhoeveden
authors: Nicolette
authors: N
authors: Gengel
authors: Jessica
authors: J
authors: Kleinschmit
authors: Charles
authors: C
authors: Desbordes
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D001645:Biguanides / Q000008:administration & dosage; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism*; D003847:Deoxyglucose / Q000008:administration & dosage; D006019:Glycolysis / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D011773:Pyruvates / Q000008:administration & dosage
chemical_list: D000970:Antineoplastic Agents; D001645:Biguanides; D011773:Pyruvates; C017092:bromopyruvate; D003847:Deoxyglucose
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