title: Analysis of developmental control genes using virus-induced gene silencing.
abstract: A consistent challenge in studying the evolution of developmental processes has been the problem of explicitly assessing the function of developmental control genes in diverse species. In recent years, virus-induced gene silencing (VIGS) has proved to be remarkably adaptable and efficient in silencing developmental control genes in species across the angiosperms. Here we describe proven protocols for Nicotiana benthamiana and Papaver somniferum, representing a core and basal eudicot species.
authors: Laboratory of Plant Systematics, Institute of Botany and Microbiology, Leuven, Belgium.
authors: Koen
authors: K
authors: Geuten
authors: Tom
authors: T
authors: Viaene
authors: Dries
authors: D
authors: Vekemans
authors: Sofia
authors: S
authors: Kourmpetli
authors: Sinead
authors: S
authors: Drea
mesh_terms: D016960:Agrobacterium tumefaciens / Q000821:virology; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques / Q000379:methods; D005822:Genetic Vectors; D048788:Growth and Development / Q000235:genetics; D010207:Papaver / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D014170:Transformation, Genetic