title: An improved protocol for better detection of protein using 8-anilino-1-naphthalenesulfonate.
abstract: This study describes the optimum conditions at the staining time and the signal intensity for using 8-anilino-1-naphthalenesulfonate (ANS) as a fluorescent probe to detect proteins in SDS-PAGE. Using the optimized protocol, protein can be easily detected by short time fixing (20 min) and washing (2 × 5 min), followed by 10 min of staining. As low as 1-2 ng of the protein band can be detected, approximately thirty-fold higher than that of the original protocol. Furthermore, the compatibility of the staining method with MS was also explored by comparing the peptide mass fingerprinting results data of serial dilutions of BSA and ovalbumin stained by ANS with SYPRO Ruby.
authors: Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, P. R. China.
authors: Weide
authors: W
authors: Ma
authors: Guanghui
authors: G
authors: Zhu
authors: Ting
authors: T
authors: Zhao
authors: Weitao
authors: W
authors: Cong
authors: Weijian
authors: W
authors: Ye
authors: Litai
authors: L
authors: Jin
mesh_terms: D000817:Anilino Naphthalenesulfonates / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000191:economics / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011817:Rabbits; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: C515594:8-anilino-1-naphthalenesulfonic acid; D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D011506:Proteins