title: Adverse effect of tetracycline and doxycycline on testicular tissue and sperm parameters in CD1 outbred mice.
abstract: Tetracycline and doxycycline are commonly used antibiotics in acne treatment during puberty in humans. The long-term effect of these antibiotics on male reproductive tract development has not been fully elucidated. For this reason we tested the effect of antibiotics on the reproductive parameters of mice males during puberty with the therapeutic dose used in humans, and with lower and higher doses. The outbred mouse strain CD1 with higher heterozygosity was exposed for 14 days at puberty. Adult males at the age of 70 days were used for the measurements. We observed a significant decrease in anogenital distance and thickness of the seminiferous epithelium in the treated animals. Pathological changes in the testes had an impact on sperm quality; a higher number of sperm positively stained with Annexin V and TUNEL and a lower number of acrosome-intact sperm was detected. In conclusion, the treatment of male mice with antibiotics in puberty led to long-lasting effects on reproductive organs and spermatozoa in adult males.
authors: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
authors: Fatima
authors: F
authors: Elzeinová
authors: Jana
authors: J
authors: Pěknicová
authors: Lukáš
authors: L
authors: Děd
authors: Alena
authors: A
authors: Kubátová
authors: Hasmik
authors: H
authors: Margaryan
authors: Andriy
authors: A
authors: Dorosh
authors: Peter
authors: P
authors: Makovický
authors: Radko
authors: R
authors: Rajmon
mesh_terms: D000375:Aging / Q000187:drug effects / Q000473:pathology; D000818:Animals; D021461:Animals, Outbred Strains; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004318:Doxycycline / Q000008:administration & dosage / Q000009:adverse effects*; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D013752:Tetracycline / Q000008:administration & dosage / Q000009:adverse effects*
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline; D004318:Doxycycline
keywords: Apoptosis; Doxycycline; Puberty; Sperm; Testes; Tetracycline