title: Primary esophageal and gastro-esophageal junction cancer xenograft models: clinicopathological features and engraftment.
abstract: There are very few xenograft models available for the study of esophageal (E) and gastro-esophageal junction (GEJ) cancer. Using a NOD/SCID model, we implanted 90 primary E and GEJ tumors resected from patients and six endoscopic biopsy specimens. Of 69 resected tumors with histologically confirmed viable adenocarcinoma or squamous cell carcinoma, 22 (32%) was engrafted. One of 11 tumors, considered to have had a complete pathological response to neo-adjuvant chemo-radiation, also engrafted. Of the 23 patients whose tumors were engrafted, 65% were male; 30% were early stage while 70% were late stage; 22% received neo-adjuvant chemo-radiation; 61% were GEJ cancers. Engraftment occurred in 18/54 (33%) adenocarcinomas and 5/16 (31%) squamous cell carcinomas. Small endoscopic biopsy tissue had a 50% (3/6) engraftment rate. Of the factors analyzed, pretreatment with chemo-radiation and well/moderate differentiation showed significantly lower correlation with engraftment (P<0.05). In the subset of patients who did not receive neo-adjuvant chemo-radiation, 18/41 (44%) engrafted compared with those with pretreatment where 5/29 (17%, P=0.02) engrafted. Primary xenograft lines may be continued through 4-12 passages. Xenografts maintained similar histology and morphological characteristics with only minor variations even after multiple passaging in most instances.
authors: Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.
authors: Lorin
authors: L
authors: Dodbiba
authors: Jennifer
authors: J
authors: Teichman
authors: Andrew
authors: A
authors: Fleet
authors: Henry
authors: H
authors: Thai
authors: Bin
authors: B
authors: Sun
authors: Devang
authors: D
authors: Panchal
authors: Devalben
authors: D
authors: Patel
authors: Alvina
authors: A
authors: Tse
authors: Zhuo
authors: Z
authors: Chen
authors: Olusola O
authors: OO
authors: Faluyi
authors: Daniel J
authors: DJ
authors: Renouf
authors: Hala
authors: H
authors: Girgis
authors: Bizhan
authors: B
authors: Bandarchi
authors: Joerg
authors: J
authors: Schwock
authors: Wei
authors: W
authors: Xu
authors: Robert G
authors: RG
authors: Bristow
authors: Ming-Sound
authors: MS
authors: Tsao
authors: Gail E
authors: GE
authors: Darling
authors: Laurie E
authors: LE
authors: Ailles
authors: Hala
authors: H
authors: El-Zimaity
authors: Geoffrey
authors: G
authors: Liu
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000473:pathology*; D005260:Female; D006085:Graft Survival; D006801:Humans; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays