title: Determination of salicylic acid in human serum and urine samples by high-performance liquid chromatography with post-column Ru(bipy)3(2+)-Ce(SO4)2 chemiluminescence detection.
abstract: A sensitive, novel and rapid chemiluminescence (CL) method combined with high-performance liquid chromatography (HPLC) separation for the determination of salicylic acid (SA) is described in this work. The method was based on the fact that SA could significantly enhance the CL of the reaction of cerium sulfate and the tris(2,2-bipyridyl) ruthenium(II) CL system in the presence of acid. Under the optimal conditions, the CL intensity was linear over concentrations of SA in the range of 0.02-10 × 10(-6) g/mL, with a detection limit of 8 × 10(-9) g/mL (S/N = 3). Also, the relative standard detection was 2.2% for 1.0 × 10(-7) g/mL (n = 11). The proposed method has been successfully applied to the analysis of SA in human serum samples and urine samples with satisfactory results.
authors: School of Chemistry and Chemical Engineering of Southwest University, Chongqing, P. R. China.
authors: Xingyu
authors: X
authors: Long
authors: Funan
authors: F
authors: Chen
authors: Mao
authors: M
authors: Deng
mesh_terms: D002586:Cesium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056831:Coordination Complexes; D006801:Humans; D016014:Linear Models; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds / Q000737:chemistry*; D020156:Salicylic Acid / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D017975:Ruthenium Compounds
chemical_list: D002586:Cesium; D056831:Coordination Complexes; D009942:Organometallic Compounds; D020156:Salicylic Acid; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C025407:cesium sulfate