title: Sensitive detection of 3'-hydroxy-stanozolol glucuronide by liquid chromatography-tandem mass spectrometry.
abstract: Stanozolol is one of the most frequently detected anabolic steroids in doping control samples. This compound is metabolized to a large extent and its metabolites can be detected in urine much longer than the parent compound. The main stanozolol metabolites are excreted in urine as glucuronide conjugates and 3'-hydroxy-stanozolol glucuronide (3STANG) is one of the most important in human urine. Therefore enzymatic hydrolysis is usually applied prior to extraction. In this article a method for the sensitive detection of intact 3'-hydroxy-stanozolol glucuronide, by liquid chromatography tandem mass spectrometry, is described. The method takes advantage of an easy and fast sample preparation based on a single solid-phase extraction avoiding enzymatic hydrolysis or derivatization. It allows to detect stanozolol abuse in human urine at 25pgmL(-1). The method was validated according to Eurachem guidelines. The matrix effect, expressed as ion enhancement was +14%. The extraction recovery of the method was 93%. The limit of detection (LOD), whereby all WADA-criteria in chromatography and mass spectrometry are fulfilled, was determined at 50pgmL(-1). Application of the method to an excretion study revealed that the 3'-hydroxy-stanozolol glucuronide could be confirmed for 10 days after oral administration of 2mg of stanozolol, prolonging detection times compared to other metabolites and methodologies by almost 50%.
authors: DoCoLab, Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Technologiepark 30, B-9052 Zwijnaarde, Belgium. Eva.TudelaPalomar@UGent.be
authors: E
authors: E
authors: Tudela
authors: K
authors: K
authors: Deventer
authors: P
authors: P
authors: Van Eenoo
mesh_terms: D000328:Adult; D045930:Anabolic Agents / Q000652:urine*; D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods*; D004300:Doping in Sports; D006801:Humans; D057230:Limit of Detection; D008297:Male; D052616:Solid Phase Extraction / Q000191:economics / Q000379:methods; D013197:Stanozolol / Q000031:analogs & derivatives* / Q000652:urine; D015813:Substance Abuse Detection / Q000191:economics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D013997:Time Factors
chemical_list: D045930:Anabolic Agents; C425489:3'-hydroxystanozolol; D013197:Stanozolol