title: Pathfinding of corticothalamic axons relies on a rendezvous with thalamic projections.
abstract: Major outputs of the neocortex are conveyed by corticothalamic axons (CTAs), which form reciprocal connections with thalamocortical axons, and corticosubcerebral axons (CSAs) headed to more caudal parts of the nervous system. Previous findings establish that transcriptional programs define cortical neuron identity and suggest that CTAs and thalamic axons may guide each other, but the mechanisms governing CTA versus CSA pathfinding remain elusive. Here, we show that thalamocortical axons are required to guide pioneer CTAs away from a default CSA-like trajectory. This process relies on a hold in the progression of cortical axons, or waiting period, during which thalamic projections navigate toward cortical axons. At the molecular level, Sema3E/PlexinD1 signaling in pioneer cortical neurons mediates a "waiting signal" required to orchestrate the mandatory meeting with reciprocal thalamic axons. Our study reveals that temporal control of axonal progression contributes to spatial pathfinding of cortical projections and opens perspectives on brain wiring.
authors: Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, F-75005 Paris, France.
authors: Marie
authors: M
authors: Deck
authors: Ludmilla
authors: L
authors: Lokmane
authors: Sophie
authors: S
authors: Chauvet
authors: Caroline
authors: C
authors: Mailhes
authors: Maryama
authors: M
authors: Keita
authors: Mathieu
authors: M
authors: Niquille
authors: Michio
authors: M
authors: Yoshida
authors: Yutaka
authors: Y
authors: Yoshida
authors: Cécile
authors: C
authors: Lebrand
authors: Fanny
authors: F
authors: Mann
authors: Elizabeth A
authors: EA
authors: Grove
authors: Sonia
authors: S
authors: Garel
mesh_terms: D000367:Age Factors; D000818:Animals; D001369:Axons / Q000502:physiology; D019521:Body Patterning / Q000235:genetics; D064032:Calbindin 2; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D058969:Contactin 2 / Q000378:metabolism; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D039841:Leukocyte L1 Antigen Complex / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009434:Neural Pathways / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism; D050809:POU Domain Factors / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D039961:Semaphorins; D020825:T-Box Domain Proteins; D013788:Thalamus / Q000166:cytology / Q000502:physiology*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D060509:Wnt3A Protein / Q000235:genetics; D016875:tau Proteins / Q000235:genetics; D000099260:Empty Spiracles Homeobox Proteins
chemical_list: D064032:Calbindin 2; D058969:Contactin 2; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D039841:Leukocyte L1 Antigen Complex; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D050809:POU Domain Factors; D012097:Repressor Proteins; D064030:S100 Calcium Binding Protein G; D039961:Semaphorins; D020825:T-Box Domain Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D060509:Wnt3A Protein; D016875:tau Proteins; D000099260:Empty Spiracles Homeobox Proteins; C511143:Bcl11b protein, mouse; C548675:Cntn2 protein, mouse; C473552:Plxnd1 protein, mouse; C111150:Sema3e protein, mouse; C492271:Tbr1 protein, mouse; C492170:Zfpm2 protein, mouse; C074067:Pou3f4 protein, mouse
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