title: A novel bocavirus in canine liver.
abstract: BACKGROUND
  Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease.
  
  
  RESULTS
  In this study using deep sequencing of enriched viral particles from the liver of a dog with severe hemorrhagic gastroenteritis, necrotizing vasculitis, granulomatous lymphadenitis and anuric renal failure, we identified and characterized a novel bocavirus we named Canine bocavirus 3 (CnBoV3). The three major ORFs of CnBoV3 (NS1, NP1 and VP1) shared less than 60% aa identity with those of other bocaviruses qualifying it as a novel species based on ICTV criteria. Inverse PCR showed the presence of concatemerized or circular forms of the genome in liver.
  
  
  CONCLUSIONS
  We genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples. Its role in this animal's complex disease remains to be determined.
authors: Blood Systems Research Institute, San Francisco, CA, USA.
authors: Linlin
authors: L
authors: Li
authors: Patricia A
authors: PA
authors: Pesavento
authors: Christian M
authors: CM
authors: Leutenegger
authors: Marko
authors: M
authors: Estrada
authors: Lark L
authors: LL
authors: Coffey
authors: Samia N
authors: SN
authors: Naccache
authors: Erik
authors: E
authors: Samayoa
authors: Charles
authors: C
authors: Chiu
authors: Jianming
authors: J
authors: Qiu
authors: Chunlin
authors: C
authors: Wang
authors: Xutao
authors: X
authors: Deng
authors: Eric
authors: E
authors: Delwart
mesh_terms: D000818:Animals; D001483:Base Sequence; D052660:Bocavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D059014:High-Throughput Nucleotide Sequencing; D008099:Liver / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004279:DNA, Viral
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