title: Liposomes modified with P-aminophenyl-α-D-mannopyranoside: a carrier for targeting cerebral functional regions in mice.
abstract: Targeting of intracerebral functional regions has been limited by the inability to transport drugs across the blood-brain barrier (BBB) and by poor accumulation in these regions. To overcome these hurdles, liposomes modified with P-aminophenyl-α-d-mannopyranoside (MAN) were used as a fluorescent dye carrier through the BBB and used the specific distribution of liposomes (LIP) modified with MAN (MAN-LIP) to target various functional regions of the brain. An in vitro BBB model was established to evaluate the transendothelial ability of MAN-LIP, and liposomes uptake by C6 glioma cells was analyzed by flow cytometry and live cell imaging. Liposome targeting was evaluated using in vivo and ex vivo imaging. After MAN-LIP administration, the transendothelial ability and the delivery of fluorescent dye to the brain significantly increased. MAN-LIP concentrated in the cortex at 4 h, shifting distribution to the cerebellum and brainstem at 12 h. The fluorescence intensity in the hippocampus and pontine nuclei remained high and stable over a period of 12 h. The results demonstrate that MAN-LIP is able to enhance cellular uptake in vitro and also promotes penetration through the BBB and accumulation in the brain with a distinct spatio-temporal pattern.
authors: Department of Pharmaceutics, Harbin Medical University, Daqing, China.
authors: Zhong-fei
authors: ZF
authors: Hao
authors: Yu-xia
authors: YX
authors: Cui
authors: Ming-hui
authors: MH
authors: Li
authors: Dan
authors: D
authors: Du
authors: Mei-fang
authors: MF
authors: Liu
authors: Hai-quan
authors: HQ
authors: Tao
authors: Sen
authors: S
authors: Li
authors: Fang-yuan
authors: FY
authors: Cao
authors: Ying-li
authors: YL
authors: Chen
authors: Xu-hui
authors: XH
authors: Lei
authors: Lin
authors: L
authors: Wang
authors: Da-ling
authors: DL
authors: Zhu
authors: Hai-sheng
authors: HS
authors: Peng
authors: Chuan-lu
authors: CL
authors: Jiang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004337:Drug Carriers; D004705:Endocytosis; D005434:Flow Cytometry; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry*; D008362:Mannosides / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D013997:Time Factors
chemical_list: D000814:Aniline Compounds; D003470:Culture Media; D004337:Drug Carriers; D008081:Liposomes; D008362:Mannosides; C034056:4-aminophenylmannoside