title: Increased expression of tyrosine phosphatase SHP-2 in Helicobacter pylori-infected gastric cancer.
abstract: OBJECTIVE
  To explore the alteration of tyrosine phosphatase SHP-2 protein expression in gastric cancer and to assess its prognostic values.
  
  
  METHODS
  Three hundred and five consecutive cases of gastric cancer were enrolled into this study. SHP-2 expression was carried out in 305 gastric cancer specimens, of which 83 were paired adjacent normal gastric mucus samples, using a tissue microarray immunohistochemical method. Correlations were analyzed between expression levels of SHP-2 protein and tumor parameters or clinical outcomes. Serum anti-Helicobacter pylori (H. pylori) immunoglobulin G was detected with enzyme-linked immunosorbent assay. Cox proportional hazards model was used to evaluate prognostic values by compassion of the expression levels of SHP-2 and disease-specific survivals in patients.
  
  
  RESULTS
  SHP-2 staining was found diffuse mainly in the cytoplasm and the weak staining was also observed in the nucleus in gastric mucosa cells. Thirty-two point five percent of normal epithelial specimen and 62.6% of gastric cancer specimen were identified to stain with SHP-2 antibody positively (P < 0.001). Though SHP-2 staining intensities were stronger in the H. pylori (+) group than in the H. pylori (-) group, no statistically significant difference was found in the expression levels of SHP-2 between H. pylori (+) and H. pylori (-) gastric cancer (P = 0.40). The SHP-2 expression in gastric cancer was not significantly associated with cancer stages, lymph node metastases, and distant metastasis of the tumors (P = 0.34, P = 0.17, P = 0.52). Multivariate analysis demonstrated no correlation between SHP-2 expression and disease-free survival (P = 0.86).
  
  
  CONCLUSIONS
  Increased expression of SHP-2 protein in gastric cancer specimen suggesting the aberrant up-regulation of SHP-2 protein might play an important role in the gastric carcinogenesis.
authors: Division of Clinical Epidemiology, Jilin University First Hospital, Changchun 130021, Jilin Province, China.
authors: Jing
authors: J
authors: Jiang
authors: Mei-Shan
authors: MS
authors: Jin
authors: Fei
authors: F
authors: Kong
authors: Yin-Ping
authors: YP
authors: Wang
authors: Zhi-Fang
authors: ZF
authors: Jia
authors: Dong-Hui
authors: DH
authors: Cao
authors: Hong-Xi
authors: HX
authors: Ma
authors: Jian
authors: J
authors: Suo
authors: Xue-Yuan
authors: XY
authors: Cao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D018572:Disease-Free Survival; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis / Q000201:enzymology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000032:analysis*; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D013997:Time Factors; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: D000907:Antibodies, Bacterial; D015415:Biomarkers; D014408:Biomarkers, Tumor; D007074:Immunoglobulin G; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11
keywords: Expression; Gastric cancer; Helicobacter pylori; SH2-containing protein tyrosine phosphatase 2
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