title: Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice.
abstract: The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this study, we performed functional analysis of Pid3-A4, an ortholog of Pid3 revealed by allele mining in the common wild rice A4 (Oryza rufipogon). The predicted protein encoded by Pid3-A4 shares 99.03% sequence identity with Pid3, with only nine amino-acid substitutions. In wild rice plants, Pid3-A4 is constitutively expressed, and its expression is not induced by Magnaporthe oryzae isolate Zhong-10-8-14 infection. Importantly, in transgenic plants, Pid3-A4, as compared with Pid3, displays a distinct resistance spectrum to a set of M. oryzae isolates, including those that prevail in the rice fields of Sichuan Province. Therefore, Pid3-A4 should be quite useful for the breeding of rice blast resistance, especially in southwestern China.
authors: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors: Qiming
authors: Q
authors: Lv
authors: Xiao
authors: X
authors: Xu
authors: Junjun
authors: J
authors: Shang
authors: Guanghuai
authors: G
authors: Jiang
authors: Zhiqian
authors: Z
authors: Pang
authors: Zhuangzhi
authors: Z
authors: Zhou
authors: Jing
authors: J
authors: Wang
authors: Ya
authors: Y
authors: Liu
authors: Ting
authors: T
authors: Li
authors: Xiaobing
authors: X
authors: Li
authors: Jichen
authors: J
authors: Xu
authors: Zhukuan
authors: Z
authors: Cheng
authors: Xianfeng
authors: X
authors: Zhao
authors: Shigui
authors: S
authors: Li
authors: Lihuang
authors: L
authors: Zhu
mesh_terms: D000483:Alleles; D000818:Animals; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005820:Genetic Testing; D054884:Host-Pathogen Interactions / Q000235:genetics; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins