title: From meniscus to bone: a quantitative evaluation of structure and function of the human meniscal attachments.
abstract: Meniscus efficacy at promoting joint congruity and preventing osteoarthritis hinges on enthesis integrity. Gross-scale tensile testing, histomorphometry and magnetic resonance imaging reveal significant differences between the four attachments, implying that each must endure a unique mechanical environment, which dictates their structure. However, little data exists to elucidate how these interfaces have adapted to their complex loading environment, particularly on a relevant scale, as the enthesis transitions through several unique zones in less than a millimeter. In our study we leveraged nanoindentation to determine viscoelastic material properties through the transition zones. Additionally, we employed histological techniques to evaluate the enthesis structure, including collagen organization and interdigitation morphometry. Mechanical evaluation revealed the medial posterior insertion site to be significantly more compliant than others. Collagen fiber orientation and dispersion as well as interdigitation morphometry were significantly different between attachments sites. These findings are clinically relevant as a disproportionate amount of enthesis failure occurs in the medial posterior attachment. Also, meniscal enthesis structure and function will need to be considered in future reparative and replacement strategies in order to recreate native meniscus mechanics and prevent osteoarthritis propagation.
authors: Department of Mechanical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
authors: Adam C
authors: AC
authors: Abraham
authors: Tammy L Haut
authors: TL
authors: Donahue
mesh_terms: D005069:Evaluation Studies as Topic; D006801:Humans; D007719:Knee Joint / Q000033:anatomy & histology* / Q000502:physiology; D008592:Menisci, Tibial / Q000033:anatomy & histology*
references: Comput Methods Biomech Biomed Engin. 2010;13(2):247-56
references: 20094931
references: Arthroscopy. 2004 Apr;20(4):373-8
references: 15067276
references: J Anat. 1991 Aug;177:127-34
references: 1769887
references: Knee Surg Sports Traumatol Arthrosc. 2011 Feb;19(2):236-41
references: 20953762
references: J Anat. 1996 Aug;189 ( Pt 1):127-33
references: 8771403
references: J Biomech. 2010 Feb 10;43(3):463-8
references: 19896669
references: J Orthop Res. 2008 May;26(5):673-84
references: 18183628
references: Ann Biomed Eng. 2008 Jan;36(1):123-31
references: 17999192
references: Scand J Med Sci Sports. 2007 Aug;17(4):303-15
references: 17490450
references: Rheum Dis Clin North Am. 2009 Aug;35(3):579-90
references: 19931804
references: Connect Tissue Res. 2010 Oct;51(5):327-36
references: 20388017
references: Anat Embryol (Berl). 1998 Apr;197(4):317-24
references: 9565324
references: J Anat. 2006 Apr;208(4):471-90
references: 16637873
references: J Bone Joint Surg Br. 1948 Nov;30B(4):664-70
references: 18894618
references: J Biomech. 2006;39(10):1842-51
references: 16024026
references: J Anat. 2010 Mar;216(3):279-91
references: 20070426
references: J Anat. 1998 Nov;193 ( Pt 4):481-94
references: 10029181
references: Z Anat Entwicklungsgesch. 1956;119(5):431-56
references: 13353255
references: J Anat. 1990 Aug;171:155-62
references: 2081702
references: Proc R Soc Lond B Biol Sci. 1981 Jul 14;212(1188):299-304
references: 6115394
references: Clin Orthop Relat Res. 1975;(109):184-92
references: 1173360
references: Clin Orthop Relat Res. 1972;89:215-9
references: 4678696
references: J Biomech. 2003 Jan;36(1):19-34
references: 12485635
references: Clin Orthop Relat Res. 1999 Oct;(367 Suppl):S254-72
references: 10546651
references: J Biomech Eng. 2006 Jun;128(3):399-408
references: 16706589
references: J Struct Biol. 2001 Oct;136(1):46-52
references: 11858706
references: Biophys J. 2009 Aug 19;97(4):976-85
references: 19686644
references: J Knee Surg. 2009 Oct;22(4):347-57
references: 19902731
references: J Biomech Eng. 1998 Aug;120(4):537-40
references: 10412427
references: Biophys J. 1997 Oct;73(4):2164-72
references: 9336212
references: J Biomed Mater Res. 1999 Apr;45(1):48-54
references: 10397957
references: J Bone Joint Surg Br. 1999 Jan;81(1):37-41
references: 10067999
references: Eur J Radiol. 2012 Aug;81(8):1839-45
references: 21684704
references: J Mech Behav Biomed Mater. 2009 Aug;2(4):339-47
references: 19627840
references: J Biomech Eng. 2003 Oct;125(5):726-31
references: 14618932
references: Australas Radiol. 2006 Aug;50(4):306-13
references: 16884414
references: AJR Am J Roentgenol. 2004 Jul;183(1):17-23
references: 15208101
references: J Orthop Res. 2003 May;21(3):413-9
references: 12706013
references: Knee. 2011 Dec;18(6):396-401
references: 21493071
references: Anal Quant Cytol Histol. 2001 Aug;23(4):291-9
references: 11531144
references: Ann Rheum Dis. 1973 Jul;32(4):364-78
references: 4269430
references: J Anat. 1994 Dec;185 ( Pt 3):663-7
references: 7649801