title: Nepem-211 ion exchange conductive membrane immobilized tris(2,2´-bipyridyl) ruthenium(II) electrogenerated chemiluminescence flow sensor for high-performance liquid chromatography and its application.
abstract: We developed a sensitive and robust electrogenerated chemiluminescence (ECL) flow sensor based on Ru(bpy)3(2+) immobilized with a Nepem-211 perfluorinated ion exchange conductance membrane, which has robustness and stability under a wide range of chemical and physical conditions, good electrical conductivity, isotropy and a high exchange capacity for immobilization of Ru(bpy)3(2+). The flow sensor has been used as a post-column detector in high-performance liquid chromatography for determination of erythromycin and clarithromycin in honey and pork, and tricyclic antidepressant drugs in human urine. Under optimal conditions, the linear ranges were 0.03-26 ng/μL and 0.01-1 ng/μL for macrolides and tricyclic antidepressant drugs, respectively. The detection limits were 0.02, 0.01, 0.01, 0.06 and 0.003 ng/μL for erythromycin, clarithromycin, doxepin, amitriptyline and clomipramine, respectively. There is no post-column reagent addition. In addition to the conservation expensive reagents, the experimental setup was simplified. The flow sensor was used for 2 years with high sensitivity and stability.
authors: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
authors: Yongbo
authors: Y
authors: Li
authors: Zhujun
authors: Z
authors: Zhang
mesh_terms: D000639:Amitriptyline / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D002997:Clomipramine / Q000652:urine; D056831:Coordination Complexes; D004316:Doxepin / Q000652:urine; D004553:Electric Conductivity; D055664:Electrochemical Techniques / Q000295:instrumentation; D064368:Healthy Volunteers; D006801:Humans; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D007474:Ion Exchange; D008163:Luminescent Measurements / Q000295:instrumentation; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D017975:Ruthenium Compounds
chemical_list: D000639:Amitriptyline; D002997:Clomipramine; D056831:Coordination Complexes; D004316:Doxepin; D006845:Hydrocarbons, Fluorinated; D009942:Organometallic Compounds; C588154:nepem-211; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds
keywords: Tris(2, 2´-bipyridyl)ruthenium(II); application; electrogenerated chemiluminescence; flow sensor; high-performance liquid chromatography