title: Dilute acid pretreatment of lignocellulose for whole slurry ethanol fermentation.
abstract: Dilute sulfuric acid pretreatment of oil palm empty fruit bunches (EFB) followed by the whole slurry fermentation of the pretreated EFB slurry was investigated. The optimized pretreatment conditions were at 1% (w/v) sulfuric acid with 3 min ramping to 190 °C in a microwave digester. Pretreated and washed EFB exhibited enzymatic digestibility of 88.5% of theoretical glucose yield after 48 h of hydrolysis. When the whole slurry of pretreated and neutralized EFB was used in simultaneous saccharification and fermentation (SSF) using cellulase and Saccharomyces cerevisiae, sulfuric acid-pretreated EFB resulted in 52.5% of theoretical ethanol yield based on total glucan in the untreated initial EFB after 72 h of SSF. When pretreated EFB slurry was treated with activated carbon before subjecting to SSF, the SSF furnished 87.5% ethanol yield based on the initial glucan content in untreated EFB (after 48 h of SSF).
authors: School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors: Young Hoon
authors: YH
authors: Jung
authors: In Jung
authors: IJ
authors: Kim
authors: Hyun Kyung
authors: HK
authors: Kim
authors: Kyoung Heon
authors: KH
authors: Kim
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism; D002606:Charcoal; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005638:Fruit / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000096:biosynthesis*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D008872:Microwaves; D012441:Saccharomyces cerevisiae / Q000254:growth & development; D013464:Sulfuric Acids / Q000378:metabolism*
chemical_list: D013464:Sulfuric Acids; C036909:lignocellulose; D002606:Charcoal; D000431:Ethanol; D008031:Lignin; D002480:Cellulase; D005947:Glucose; C033158:sulfuric acid