title: The therapeutic effect of negative pressure in treating femoral head necrosis in rabbits.
abstract: Because negative pressure can stimulate vascular proliferation, improve blood circulation and promote osteogenic differentiation of bone marrow stromal cells, we investigated the therapeutic effect of negative pressure on femoral head necrosis (FHN) in a rabbit model. Animals were divided into four groups (n = 60/group): [1] model control, [2] core decompression, [3] negative pressure and [4] normal control groups. Histological investigation revealed that at 4 and 8 weeks postoperatively, improvements were observed in trabecular bone shape, empty lacunae and numbers of bone marrow hematopoietic cells and fat cells in the negative pressure group compared to the core decompression group. At week 8, there were no significant differences between the negative pressure and normal control groups. Immunohistochemistry staining revealed higher expression of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) in the femoral heads in the negative pressure group compared with the core decompression group. Transmission electron microscopy revealed that cell organelles were further developed in the negative pressure group compared with the core decompression group. Microvascular ink staining revealed an increased number of bone marrow ink-stained blood vessels, a thicker vascular lumen and increased microvascular density in the negative pressure group relative to the core decompression group. Real-time polymerase chain reaction revealed that expression levels of both VEGF and BMP-2 were higher in the negative pressure group compared with the core decompression group. In summary, negative pressure has a therapeutic effect on FHN. This effect is superior to core decompression, indicating that negative pressure is a potentially valuable method for treating early FHN.
authors: Department of Orthopedics, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China. zyingang@mail.xjtu.edu.cn
authors: Yin-gang
authors: YG
authors: Zhang
authors: Xuezhi
authors: X
authors: Wang
authors: Zhi
authors: Z
authors: Yang
authors: Hong
authors: H
authors: Zhang
authors: Miao
authors: M
authors: Liu
authors: Yushen
authors: Y
authors: Qiu
authors: Xiong
authors: X
authors: Guo
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D019299:Decompression, Surgical; D004195:Disease Models, Animal; D005270:Femur Head / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D005271:Femur Head Necrosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D008297:Male; D055806:Microvessels / Q000378:metabolism; D054843:Negative-Pressure Wound Therapy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors; D042442:Vascular Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2; D012333:RNA, Messenger; D042442:Vascular Endothelial Growth Factors
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