title: The effectiveness of the oncolytic activity induced by Ad5/F35 adenoviral vector is dependent on the cumulative cellular conditions of survival and autophagy.
abstract: To overcome the poor tumor transduction efficiency of adenovirus serotype 5 (Ad5) observed in several types of cancer, the fiber region of Ad5, apart from its tail, was replaced by adenovirus serotype 35 (Ad35). The chimeric Ad5/F35 adenoviral vector did not exhibit any significant enhancement of transduction efficiency. CD46, a receptor for Ad35, was expressed in relatively small amounts in most of the cancer cells examined. Therefore, we investigated the pivotal factor(s) that render cancer cells susceptible to transduction. We discovered that the tumor transduction efficiency of Ad5/F35 was enhanced in the presence of rapamycin, an autophagy inducer, in some cancer cells. Analysis of survival potential and cell proliferation rates revealed that Ad5/F35 exerted a more pronounced oncolytic effect in cancer cells with higher survival potential in the presence of rapamycin.
authors: Institute for Cancer Research, College of Medicine, Yonsei University, Seoul, Republic of Korea.
authors: So Y
authors: SY
authors: Kim
authors: Sujin
authors: S
authors: Kang
authors: Jae J
authors: JJ
authors: Song
authors: Joo-Hang
authors: JH
authors: Kim
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001343:Autophagy; D045744:Cell Line, Tumor; D002470:Cell Survival; D003587:Cytomegalovirus / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D051916:Membrane Cofactor Protein / Q000096:biosynthesis / Q000235:genetics; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051603:Recoverin / Q000096:biosynthesis / Q000235:genetics; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D014161:Transduction, Genetic; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; C494811:BCL2L1 protein, human; C497857:CD46 protein, human; D051916:Membrane Cofactor Protein; D019253:Proto-Oncogene Proteins c-bcl-2; C496731:RCVRN protein, human; D051020:bcl-X Protein; D051603:Recoverin; C546842:MTOR protein, human; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus