title: Adenovirus-mediated interferon-γ gene therapy induced human pancreatic carcinoma Capan-2 cell apoptosis in vitro and in vivo.
abstract: Pancreatic cancer is one of the most lethal human malignancies with a very low 5-year survival rate, which highlights urgent needs for more effective therapeutic strategies. In this study, we examined the potential therapeutic effects of an adenovirus encoding human interferon gamma (Ad-IFNγ) on pancreatic carcinoma cells Capan-2 in vitro and in vivo. The results indicated that Ad-IFNγ could significantly inhibit tumor cell growth via inducing cell apoptosis. After infection, IFNγ expressed durably and stably in xenografts, predominantly in tumor tissue, while much less in blood and liver. Thus, adenovirus-mediated intratumoral injection of human IFNγ gene could be an effective gene therapeutic system for the treatment of pancreatic carcinoma.
authors: Department of Medical Oncology, Zhejiang Cancer Hospital, HangZhou, 310022, China.
authors: Fa-Jun
authors: FJ
authors: Xie
authors: Peng
authors: P
authors: Zhao
authors: Yi-Ping
authors: YP
authors: Zhang
authors: Fei-Ye
authors: FY
authors: Liu
authors: Xi-Lin
authors: XL
authors: Nie
authors: Ying-Hui
authors: YH
authors: Zhu
authors: Xin-Min
authors: XM
authors: Yu
authors: Qiu-Qing
authors: QQ
authors: Zheng
authors: Wei-Min
authors: WM
authors: Mao
authors: Hong-Yang
authors: HY
authors: Lu
authors: Hong
authors: H
authors: Wei
authors: Wenlin
authors: W
authors: Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
chemical_list: C573415:IFNG protein, human; D007371:Interferon-gamma