title: Simultaneous determination of arctiin and its metabolites in rat urine and feces by HPLC.
abstract: Arctiin, an important lignan compound in Fructus Arctii, has been reported to possess various kinds of bioactivities. Previous studies on the pharmacokinetic of arctiin after oral administration showed that it had a rapid absorption phase followed by a sharp but lasting disappearance. To gain deep insight into the action mechanism of arctiin, the excretion and metabolism of arctiin in vivo should be further studied. In this paper, three metabolites were isolated and identified in rat feces as (-)-enterolactone (M-1), (-)-arctigenin (M-2) and [(2R,3R)-2-(3'-hydroxybenzyl)-3-(3″,4″-dimethoxybenzyl)-butyrolactone] (M-3). Based on the structures of three metabolites, possible metabolic pathways of arctiin in rats are proposed. At the same time, the cumulative excretion rate of arctiin and its metabolites in rat urine and feces were determined, indicating that arctiin was excreted 19.84% in urine and 1.80% in feces, respectively, enterolactone, the most main metabolite, was excreted 35.80% in feces. These results provide very important information for understanding the metabolism and excretion of arctiin in vivo and speculating its action mechanism, they can provide useful information and reference for further metabolic investigations on arctiin in humans.
authors: School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
authors: Wei
authors: W
authors: Wang
authors: Qiang
authors: Q
authors: Pan
authors: Xue-Ying
authors: XY
authors: Han
authors: Jing
authors: J
authors: Wang
authors: Ri-Qiu
authors: RQ
authors: Tan
authors: Fan
authors: F
authors: He
authors: De-Qiang
authors: DQ
authors: Dou
authors: Ting-Guo
authors: TG
authors: Kang
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D000818:Animals; D031170:Arctium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D005638:Fruit; D005663:Furans / Q000378:metabolism* / Q000652:urine; D005960:Glucosides / Q000378:metabolism* / Q000652:urine; D017705:Lignans / Q000378:metabolism* / Q000652:urine; D008297:Male; D010936:Plant Extracts / Q000378:metabolism* / Q000652:urine; D017207:Rats, Sprague-Dawley
chemical_list: D005663:Furans; D005960:Glucosides; D017705:Lignans; D010936:Plant Extracts; D015107:4-Butyrolactone; C077992:arctiin; C071942:arctigenin; C029497:2,3-bis(3'-hydroxybenzyl)butyrolactone