title: Polyphenolics composition of the leaves of Zanthoxylum bungeanum Maxim. grown in Hebei, China, and their radical scavenging activities.
abstract: High performance liquid chromatography coupled with tandem mass spectrometry with electrospray ionization and negative ion detection (HPLC-ESI-MS/MS) was used to identify the polyphenols from the leaves of a traditional vegetable and spice,  Zanthoxylum bungeanum  Maxim., in China by comparison of the retention times and mass spectral fragment with standard substances or related literature. A phenolic acid glucoside, two acids, and eight flavonoids were determined including vanillic acid-4-glucoside (22.75 μg/g, dry weight of leaves), quinic acid (58.58 μg/g), chlorogenic acid (2515.96 μg/g), epicatechin (77.80 μg/g), 5-feruloyquinic acid (16.63 μg/g), syringetin-3-glucoside (103.23 μg/g), rutin (89.41 μg/g), hyperoside (886.36 μg/g), quercetin-3-arabinoside (118.75 μg/g), quercitrin (645.82 μg/g), and isorhamnetin-3-glucoside (104.27 μg/g). The obtained polyphenols show good radical scavenging activities in DPPH and superoxide anion. Through the intracellular antioxidation assay, the result shows that the polyphenols could reduce the ROS in HT-29 cells without cell toxicity.
authors: Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, People's Republic of China.
authors: Li-Chen
authors: LC
authors: Yang
authors: Rong
authors: R
authors: Li
authors: Jin
authors: J
authors: Tan
authors: Zi-Tao
authors: ZT
authors: Jiang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002681:China; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry* / Q000494:pharmacology; D053719:Tandem Mass Spectrometry; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D059808:Polyphenols