title: Glucose 6P binds and activates HlyIIR to repress Bacillus cereus haemolysin hlyII gene expression.
abstract: Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation despite the recruitment of phagocytic cells. We have previously shown that B. cereus Haemolysin II (HlyII) induces macrophage cell death by apoptosis. In this work, we investigated the regulation of the hlyII gene. We show that HlyIIR, the negative regulator of hlyII expression in B. cereus, is especially active during the early bacterial growth phase. We demonstrate that glucose 6P directly binds to HlyIIR and enhances its activity at a post-transcriptional level. Glucose 6P activates HlyIIR, increasing its capacity to bind to its DNA-box located upstream of the hlyII gene, inhibiting its expression. Thus, hlyII expression is modulated by the availability of glucose. As HlyII induces haemocyte and macrophage death, two cell types that play a role in the sequestration of nutrients upon infection, HlyII may induce host cell death to allow the bacteria to gain access to carbon sources that are essential components for bacterial growth.
authors: INRA, Unité MICALIS UMR-1319, La Minière, Guyancourt, France.
authors: Elisabeth
authors: E
authors: Guillemet
authors: Seav-Ly
authors: SL
authors: Tran
authors: Céline
authors: C
authors: Cadot
authors: Didier
authors: D
authors: Rognan
authors: Didier
authors: D
authors: Lereclus
authors: Nalini
authors: N
authors: Ramarao
mesh_terms: D001409:Bacillus cereus / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000235:genetics / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional
chemical_list: C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D006460:Hemolysin Proteins; D005952:Glucose-6-Phosphatase; D005947:Glucose
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