title: Effect of moisture-damage intervention on the immunotoxic potential and microbial content of airborne particles and on occupants' upper airway inflammatory responses.
abstract: This intervention study evaluated the effect of moisture-damage repairs on the exposure and on the upper airway inflammatory responses of the occupants. The airborne microbial exposure was followed by quantitative PCR analyses of 13 microbial species in repeated long-term indoor air samples before (N = 26) and after (N = 28) repairs of the school building. Airborne particulate matter was collected similarly from the same premises (before N = 25, after N = 34) for determination of nitric oxide (NO), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6), measured in the cell culture medium of mouse macrophages. NO, TNFα, IL-6, and IL-4 were also analyzed in the nasal lavage (NAL) samples of the occupants (N = 13) to characterize their upper airway inflammatory responses during the exposure and after its cessation. After the repairs, concentrations of the measured airborne microbes decreased, the difference being significant for six of 13 species. After renovation, airborne particulate matter also caused significantly lower production of IL-6 and TNF-α in mouse macrophages than the material collected before the renovation. The concentration of IL-4 in the NAL samples was significantly lower after the renovation. These results show that the inflammatory potential of the airborne material decreases after intensive repair of the moisture damage.
authors: Department of Environmental Science, University of Eastern Finland, Kuopio, Finland. marjut.roponen@uef.fi
authors: M
authors: M
authors: Roponen
authors: T
authors: T
authors: Meklin
authors: H
authors: H
authors: Rintala
authors: A
authors: A
authors: Hyvärinen
authors: M-R
authors: MR
authors: Hirvonen
mesh_terms: D000391:Air Microbiology; D000818:Animals; D002460:Cell Line; D004781:Environmental Exposure / Q000009:adverse effects*; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D009666:Nose / Q000276:immunology*; D020545:Respiratory Mucosa / Q000276:immunology*; D013302:Streptomyces / Q000302:isolation & purification
keywords: Cytokine; Fungi; In vitro; Inflammation; Moisture-damage intervention; Nasal lavage