title: The primacy of platelet-rich fibrin on bone regeneration of various grafts in rabbit's calvarial defects.
abstract: OBJECTIVE
  This study investigated the effect of platelet-rich fibrin (PRF) on bone regeneration of various grafting materials in rabbit calvarial defects.
  
  
  METHODS
  Two bicortical skull defects were prepared in 20 New Zealand white rabbits; 10 rabbits were treated with PRF and the other 10 were non-PRF. In both groups, autogenous bone was compare to empty defects in 5 rabbits and the composite of autogenous bone and deproteinized bovine bone versus deproteinized bovine bone (DBB) in the other five. The animals were sacrificed at 8 weeks. Bone formation was assessed by radiographic densitometry and histomorphometric analysis.
  
  
  RESULTS
  The mean optical density (OD) and histomorphometric analysis (HA) of the percentage of new bone showed that the PRF groups were significantly higher than the non-PRF groups in the autogenous bone graft (OD: 0.60 ± 0.19 vs 0.36 ± 0.03; HA: 38.03 ± 4.23 vs 26.21 ± 10.58) and the empty defect (OD: 0.29 ± 0.06 vs 0.11 ± 0.06; HA: 18.81 ± 9.27 vs 6.24 ± 5.01), but not in the DBB group (OD: 1.18 ± 0.17 vs 1.07 ± 0.05; HA: 13.067 ± 3.64 vs 9.63 ± 5.47) and the composite group (OD: 0.81 ± 0.15 vs 0.91 ± 0.05; HA: 22.63 ± 3.61 vs 21.29 ± 3.52).
  
  
  CONCLUSIONS
  PRF had a positive effect on bone formation when used alone or combined with autogenous bone, but not with deproteinized bovine bone.
authors: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand. Electronic address: prisana.p@gmail.com.
authors: Prisana
authors: P
authors: Pripatnanont
authors: Thongchai
authors: T
authors: Nuntanaranont
authors: Surapong
authors: S
authors: Vongvatcharanon
authors: Kingkaew
authors: K
authors: Phurisat
mesh_terms: D015502:Absorptiometry, Photon / Q000379:methods; D000818:Animals; D064592:Autografts / Q000000981:diagnostic imaging / Q000473:pathology / Q000637:transplantation; D001792:Blood Platelets / Q000502:physiology*; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D003399:Craniotomy / Q000379:methods; D005337:Fibrin / Q000627:therapeutic use*; D064593:Heterografts / Q000000981:diagnostic imaging / Q000473:pathology / Q000637:transplantation; D008297:Male; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D010294:Parietal Bone / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D011817:Rabbits
chemical_list: D005337:Fibrin
keywords: Autogenous bone graft; Calvarial; Densitometry; Deproteinized bovine bone; Histomorphometry; Platelet-rich fibrin