title: Transbilayer dynamics of phospholipids in the plasma membrane of the Leishmania genus.
abstract: Protozoans of the Leishmania genus are the etiological agents of a wide spectrum of diseases commonly known as leishmaniases. Lipid organization of the plasma membrane of the parasite may mimic the lipid organization of mammalian apoptotic cells and play a role in phagocytosis and parasite survival in the mammal host. Here, we analyzed the phospholipid dynamics in the plasma membrane of both the L. (Leishmania) and the L. (Viannia) subgenera. We found that the activity and substrate specificity of the inward translocation machinery varied between Leishmania species. The differences in activity of inward phospholipid transport correlated with the different sensitivities of the various species towards the alkyl-phospholipid analogue miltefosine. Furthermore, all species exhibited a phospholipid scramblase activity in their plasma membranes upon stimulation with calcium ionophores. However, binding of annexin V to the parasite surface was only detected for a subpopulation of parasites during the stationary growth phase and only marginally enhanced by scramblase activation.
authors: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil.
authors: Marcos Gonzaga
authors: MG
authors: dos Santos
authors: Sandra Marcia
authors: SM
authors: Muxel
authors: Ricardo Andrade
authors: RA
authors: Zampieri
authors: Thomas Günther
authors: TG
authors: Pomorski
authors: Lucile Maria
authors: LM
authors: Floeter-Winter
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D015759:Ionomycin / Q000494:pharmacology; D007891:Leishmania / Q000187:drug effects / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism; D049294:Phospholipid Transfer Proteins / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D017304:Annexin A5; D008051:Lipid Bilayers; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D015759:Ionomycin
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