title: Establishment of an agroinoculation system for broad bean wilt virus 2.
abstract: We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.
authors: Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
authors: Go
authors: G
authors: Atsumi
authors: Reiko
authors: R
authors: Tomita
authors: Kappei
authors: K
authors: Kobayashi
authors: Ken-Taro
authors: KT
authors: Sekine
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D019181:Fabavirus / Q000235:genetics* / Q000302:isolation & purification; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016679:Genome, Viral; D030012:Gentiana / Q000821:virology*; D007564:Japan; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology; D014170:Transformation, Genetic
chemical_list: D012367:RNA, Viral