title: Simple super-resolution live-cell imaging based on diffusion-assisted Förster resonance energy transfer.
abstract: Despite the recent development of several super-resolution fluorescence microscopic techniques, there are still few techniques that can be readily employed in conventional imaging systems. We present a very simple, rapid, general and cost-efficient super-resolution imaging method, which can be directly employed in a simple fluorescent imaging system with general fluorophores. Based on diffusion-assisted Förster resonance energy transfer (FRET), fluorescent donor molecules that label specific target structures can be stochastically quenched by diffusing acceptor molecules, thereby temporally separating otherwise spatially overlapped fluorescence signals and allowing super-resolution imaging. The proposed method provides two- to three-fold-enhancement in spatial resolution, a significant optical sectioning property, and favorable temporal resolution in live-cell imaging. We demonstrate super-resolution live-cell dynamic imaging using general fluorophores in a standard epi-fluorescence microscope with light-emitting diode (LED) illumination. Due to the simplicity of this approach, we expect that the proposed method will prove an attractive option for super-resolution imaging.
authors: Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Sangyeon
authors: S
authors: Cho
authors: Jaeduck
authors: J
authors: Jang
authors: Chaeyeon
authors: C
authors: Song
authors: Heeyoung
authors: H
authors: Lee
authors: Prabhakar
authors: P
authors: Ganesan
authors: Tae-Young
authors: TY
authors: Yoon
authors: Mahn Won
authors: MW
authors: Kim
authors: Myung Chul
authors: MC
authors: Choi
authors: Hyotcherl
authors: H
authors: Ihee
authors: Won Do
authors: WD
authors: Heo
authors: YongKeun
authors: Y
authors: Park
mesh_terms: D016475:3T3 Cells; D000199:Actins / Q000032:analysis*; D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D007382:Intermediate Filaments / Q000648:ultrastructure*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D008870:Microtubules / Q000648:ultrastructure*
chemical_list: D000199:Actins; D005456:Fluorescent Dyes
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