title: Group II activators of G-protein signaling: monitoring the interaction of Gα with the G-protein regulatory motif in the intact cell.
abstract: The G-protein regulatory (GPR) motif serves as a docking site for Gαi-GDP free of Gβγ. The GPR-Gα complex may function at the cell cortex and/or at intracellular sites. GPR proteins include the Group II Activators of G-protein signaling identified in a functional screen for receptor-independent activators of G-protein signaling (GPSM1-3, RGS12) each of which contain 1-4 GPR motifs. GPR motifs are also found in PCP2/L7(GPSM4), Rap1-Gap1 Transcript Variant 1, and RGS14. While the biochemistry of the interaction of GPR proteins with purified Gα is generally understood, the dynamics of this signaling complex and its regulation within the cell remains undefined. Major questions in the field revolve around the factors that regulate the subcellular location of GPR proteins and their interaction with Gαi and other binding partners in the cell. As an initial approach to this question, we established a platform to monitor the GPR-Gαi complex in intact cells using bioluminescence resonance energy transfer.
authors: Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina, USA.
authors: Sukru Sadik
authors: SS
authors: Oner
authors: Joe B
authors: JB
authors: Blumer
authors: Stephen M
authors: SM
authors: Lanier
mesh_terms: D001665:Binding Sites; D060807:Bioluminescence Resonance Energy Transfer Techniques; D020555:GTP-Binding Protein Regulators / Q000235:genetics / Q000378:metabolism*; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases; D015335:Molecular Probes; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D015398:Signal Transduction; D014162:Transfection
chemical_list: D020555:GTP-Binding Protein Regulators; D015335:Molecular Probes; D008156:Luciferases; D019206:GTP-Binding Protein alpha Subunits, Gi-Go