title: Syringyl-rich lignin renders poplars more resistant to degradation by wood decay fungi.
abstract: In order to elucidate the effects of lignin composition on the resistance of wood to degradation by decay fungi, wood specimens from two transgenic poplar lines expressing an Arabidopsis gene encoding ferulate 5-hydroxylase (F5H) driven by the cinnimate-4-hydroxylase promoter (C4H::F5H) that increased syringyl/guaiacyl (S/G) monolignol ratios relative to those in the untransformed control wood were incubated with six different wood decay fungi. Alterations in wood weight and chemical composition were monitored over the incubation period. The results showed that transgenic poplar lines extremely rich in syringyl lignin exhibited a drastically improved resistance to degradation by all decay fungi evaluated. Lignin monomer composition and its distribution among cell types and within different cell layers were the sole wood chemistry parameters determining wood durability. Since transgenic poplars with exceedingly high syringyl contents were recalcitrant to degradation, where wood durability is a critical factor, these genotypes may offer improved performance.
authors: Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Oleksandr
authors: O
authors: Skyba
authors: Carl J
authors: CJ
authors: Douglas
authors: Shawn D
authors: SD
authors: Mansfield
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005658:Fungi / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D032107:Populus / Q000378:metabolism / Q000382:microbiology*; D011401:Promoter Regions, Genetic; D014934:Wood / Q000382:microbiology*
chemical_list: D029681:Arabidopsis Proteins; C434470:guaiacyl monolignol; D008031:Lignin; D003577:Cytochrome P-450 Enzyme System; C097486:CYP84A1 protein, Arabidopsis
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