title: Antibacterial activity and cell viability of hyaluronan fiber with silver nanoparticles.
abstract: Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.
authors: Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Studentská 95, CZ-532 10, Pardubice, Czech Republic. abdo_mohsennrc@yahoo.co
authors: A M
authors: AM
authors: Abdel-Mohsen
authors: Radim
authors: R
authors: Hrdina
authors: Ladislav
authors: L
authors: Burgert
authors: Rasha M
authors: RM
authors: Abdel-Rahman
authors: Martina
authors: M
authors: Hašová
authors: Daniela
authors: D
authors: Šmejkalová
authors: Michal
authors: M
authors: Kolář
authors: M
authors: M
authors: Pekar
authors: A S
authors: AS
authors: Aly
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D012997:Solvents; D012834:Silver; D006820:Hyaluronic Acid