title: D-amino acid oxidase-nanoparticle system: a potential novel approach for cancer enzymatic therapy.
abstract: OBJECTIVE
  The authors propose a new magnetic nanoparticle-enzyme system for cancer therapy capable of targeting the enzyme and consequently decreasing the adverse effects, meanwhile improving the patient's life quality.
  
  
  METHODS
  The authors have functionalized Fe3O4 nanoparticles with 3-amino-propyltriethoxysilane (APTES) and conjugated it to yeast D-amino acid oxidase (DAAO) by coupling this with glutaraldehyde.
  
  
  CONCLUSIONS
  The authors have tested the Fe3O4-APTES-DAAO system on three tumor cell lines. Exposed cells show, at the electron microscope level, nanoparticles on the surface of the plasma membrane and inside endocytic vesicles. Fe3O4-APTES-DAAO caused a substantial decrease of cell viability greatly augmented when D-alanine, a DAAO substrate, was added. Fe3O4-APTES-DAAO was demonstrated to be more effective than free DAAO, confirming the validity of the system in cancer therapy.
authors: Dipartimento di Biotecnologie & Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.
authors: Adriana
authors: A
authors: Bava
authors: Rosalba
authors: R
authors: Gornati
authors: Francesca
authors: F
authors: Cappellini
authors: Laura
authors: L
authors: Caldinelli
authors: Loredano
authors: L
authors: Pollegioni
authors: Giovanni
authors: G
authors: Bernardini
mesh_terms: D002470:Cell Survival / Q000502:physiology; D003605:D-Amino-Acid Oxidase / Q000737:chemistry*; D045325:HCT116 Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000628:therapy*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003605:D-Amino-Acid Oxidase