title: Approaches to enhancing the sensitivity of carbohydrate separations in capillary electrophoresis.
abstract: Electrophoresis in both capillaries (CE) and microchips (ME) is an extremely powerful liquid phase-separation technique that is indispensable for the separation of carbohydrates. It is capable of separating both small mono- and disaccharides, through to more complex oligo- and polysaccharides, with high resolution, but as with all CE and ME separations, the detection limits are often inferior to those that can be achieved with liquid chromatographic methods. One avenue to address this is to use an on-line concentration strategy. Various approaches have been developed over the past 20 years, and this chapter will highlight their application to improve the sensitivity of carbohydrate separations in both CE and ME.
authors: Australian Center for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, TAS, Australia. mcb@utas.edu.au
authors: M C
authors: MC
authors: Breadmore
mesh_terms: D002241:Carbohydrates / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D005374:Filtration / Q000379:methods; D005936:Glucans / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057625:Isotachophoresis / Q000379:methods; D046208:Microchip Analytical Procedures / Q000379:methods
chemical_list: D002241:Carbohydrates; D005936:Glucans