title: Selenoprotein S expression in the rat brain following focal cerebral ischemia.
abstract: Recent studies on cerebral ischemic stroke have demonstrated the importance of the inflammatory response. Ongoing inflammatory insults have been implicated as a secondary mechanism underlying neuronal injury induced by ischemia, and anti-inflammatory strategies have gained considerable interest. Selenoprotein S (SelS), which is an endoplasmic reticulum resident protein, is known to promote cell survival by regulating inflammation. Moreover, SelS has been shown to be responsive to ischemia in cultured astrocytes. A Finnish report revealed that a variation in the SelS gene locus is associated with a higher predisposition to ischemic stroke in humans, suggesting a crucial role for SelS in protection against brain ischemia. However, the time-course of SelS expression following cerebral ischemia in vivo remains unknown. In the present study, we show, for the first time, differential SelS expression from 3 h to 7 days after reperfusion in rats with transient focal cerebral ischemia induced by a 1-h middle cerebral artery occlusion. We found that the SelS protein level decreased in the ischemic core 3-7 days after reperfusion. Furthermore, SelS expression was upregulated in the ischemic penumbra adjacent to the ischemic core 3-7 days after reperfusion and is matched by reactive astrogliosis. Thus, we propose that the upregulation of Sels represents a reaction of astrocytes against inflammatory stimuli, and the findings of this study open a new chapter in the research of the interrelationships between SelS and cerebral ischemic stroke.
authors: Department of Neurology, Affiliated Hospital of Jining Medical University, 272029, Jining, China.
authors: Li Xia
authors: LX
authors: Liu
authors: Xue Ying
authors: XY
authors: Zhou
authors: Cheng Shan
authors: CS
authors: Li
authors: Li Qing
authors: LQ
authors: Liu
authors: Shan Ying
authors: SY
authors: Huang
authors: Sheng Nian
authors: SN
authors: Zhou
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D051140:Selenoproteins / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D051140:Selenoproteins
references: Acta Neuropathol. 2010 Jan;119(1):7-35
references: 20012068
references: Glia. 2011 Jun;59(6):959-72
references: 21456042
references: Neurochem Res. 2010 Aug;35(8):1147-55
references: 20386979
references: J Mol Neurosci. 2008 Jul;35(3):259-65
references: 18498015
references: Biochem J. 2009 Jul 29;422(1):11-22
references: 19627257
references: Nature. 2004 Jun 24;429(6994):841-7
references: 15215856
references: Curr Top Med Chem. 2009;9(14):1240-60
references: 19849665
references: BMC Neurol. 2009 Apr 28;9:18
references: 19400931
references: Cerebrovasc Dis. 2009;27(6):544-51
references: 19390179
references: Nat Genet. 2005 Nov;37(11):1234-41
references: 16227999
references: J Neurol Neurosurg Psychiatry. 2005 Oct;76(10):1366-72
references: 16170078
references: Hum Genet. 2007 Nov;122(3-4):355-65
references: 17641917
references: Curr Opin Investig Drugs. 2009 Mar;10(3):220-3
references: 19333878
references: Crit Rev Neurobiol. 2006;18(1-2):145-57
references: 17725517
references: Int J Immunopathol Pharmacol. 2006 Jul-Sep;19(3):639-46
references: 17026849
references: Stroke. 1989 Jan;20(1):84-91
references: 2643202
references: J Cell Biol. 2001 Oct 15;155(2):217-26
references: 11604419
references: Stroke. 2000 Oct;31(10):2325-9
references: 11022058
references: Trends Neurosci. 2000 Dec;23(12):618-25
references: 11137152
references: J Neuroimmunol. 2007 Mar;184(1-2):53-68
references: 17188755
references: Neurology. 1998 Jun;50(6):1722-8
references: 9633717
references: Neurosurgery. 1992 Jul;31(1):100-6; discussion 106-7
references: 1641086
references: Stroke. 2009 Apr;40(4):1458-66
references: 19246692
references: J Neuropathol Exp Neurol. 1998 Mar;57(3):231-8
references: 9600215