title: Antimicrobial peptide LL-37 attenuates LTA induced inflammatory effect in macrophages.
abstract: LL-37/hCAP-18, as the only human cathelicidin, plays an important role in inflammation. Lipoteichoic acid (LTA) is an important bacterial component of Staphylococcus aureus, which is one of the common human pathogens for severe respiratory infection with increasing morbidity in recent years. The present study is to investigate the role of LL-37 in LTA induced inflammatory reaction in macrophages. We examined TNF-α and IL-6 production after LL-37 treatment and discussed its signal transduction pathways such as p38MAPK and Akt activation in macrophages. The expression of pro-inflammatory cytokines was analyzed by quantitative real-time RT-PCR and enzyme-linked immunosorbent assay (ELISA). The LL-37 expression was determined by Western blot and immunofluorescence staining. The results showed that LL-37 was upregulated after LTA treatment. It could inhibit LTA induced p38MAPK and Akt phosphorylation and attenuate TNF-α and IL-6 production in macrophages in some specific concentration. These results suggest that LL-37 exerts an anti-inflammatory property and attenuates the pro-inflammatory cytokine release in macrophages.
authors: Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors: Yang
authors: Y
authors: Ruan
authors: Tao
authors: T
authors: Shen
authors: Yan
authors: Y
authors: Wang
authors: Man
authors: M
authors: Hou
authors: Jian
authors: J
authors: Li
authors: Tieying
authors: T
authors: Sun
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D013682:Teichoic Acids / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D054804:Cathelicidins
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D023181:Antimicrobial Cationic Peptides; D015850:Interleukin-6; D008070:Lipopolysaccharides; D013682:Teichoic Acids; D014409:Tumor Necrosis Factor-alpha; C009900:lipoteichoic acid; D048051:p38 Mitogen-Activated Protein Kinases; D054804:Cathelicidins