title: Laccase-assisted formation of bioactive chitosan/gelatin hydrogel stabilized with plant polyphenols.
abstract: Laccase-assisted simultaneous cross-linking and functionalization of chitosan/gelatin blends with phenolic compounds from Hamamelis virginiana was investigated for the development of bioactive hydrogel dressings. The potential of these hydrogels for chronic wound treatment was evaluated in vitro, assessing their antibacterial and inhibitory effect on myeloperoxidase and collagenase. Rheological studies revealed that the mechanical properties of the hydrogels were a function of the enzymatic reaction time. Stable hydrogels and resistant to lysozyme degradation were achieved after 2 h laccase reaction. The inhibitory capacity of the hydrogel for myeloperoxidase and collagenase was 32% and 79% respectively after 24 h incubation. Collagenase activity was additionally suppressed by adsorption (20%) of the enzyme onto the hydrogel. Therefore, the bioactive properties of the hydrogels were due to the effect of both released phenolic compounds and the permanently functionalized platform itself. The hydrogels showed antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus.
authors: Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politècnica de Catalunya, Passeig Ernest Lluch/Rambla Sant Nebridi s/n, 08222 Terrassa, Spain.
authors: Guillem
authors: G
authors: Rocasalbas
authors: Antonio
authors: A
authors: Francesko
authors: Sonia
authors: S
authors: Touriño
authors: Xavier
authors: X
authors: Fernández-Francos
authors: Georg M
authors: GM
authors: Guebitz
authors: Tzanko
authors: T
authors: Tzanov
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D001424:Bacterial Infections / Q000188:drug therapy; D001458:Bandages / Q000382:microbiology; D055162:Biocatalysis; D048271:Chitosan / Q000378:metabolism*; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D005780:Gelatin / Q000378:metabolism*; D029501:Hamamelis / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000378:metabolism*; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D055595:Mechanical Phenomena; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000037:antagonists & inhibitors; D059808:Polyphenols / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D055454:Trametes / Q000201:enzymology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D020100:Hydrogels; D059808:Polyphenols; D005780:Gelatin; D048271:Chitosan; D042845:Laccase; D009195:Peroxidase; D020782:Matrix Metalloproteinases