title: Comparative evolutionary genomics of medaka and three-spined stickleback fabp2a and fabp2b genes with fabp2 of zebrafish.
abstract: Here we describe the evolutionary relationship of the duplicated intestinal fatty acid binding protein genes fabp2a and fabp2b from medaka and three-spined stickleback by comparing them to the well-studied fabp2 gene from zebrafish. The duplicated fabp2 genes from medaka and three-spined stickleback consist of four exons separated by three introns, which code for a polypeptide of 132 amino acids. Fabp2a and Fabp2b of medaka and three-spined stickleback share highest sequence identity with zebrafish Fabp2. All Fabp2/FABP2 sequences from vertebrates form a distinct clade in a neighbor-joining phylogenetic tree with a robust 100% bootstrap value, which indicates that the medaka and three-spined stickleback fabp2a and fabp2b are orthologs of zebrafish fabp2. The syntenic genes of fabp2a and fabp2b from medaka and three-spined stickleback were shown to be conserved with the syntenic genes of fabp2/FABP2 from zebrafish and human, evidence that the duplicated fabp2 genes from medaka and three-spined stickleback most likely arose from the teleost-specific whole-genome duplication. The tissue-specific distribution of medaka and three-spined stickleback fabp2a and fabp2b transcripts, and zebrafish fabp2 transcripts, assayed by RT-qPCR suggests the acquisition of new function(s) by the medaka fabp2a, and the distinct evolution of fabp2b compared with fabp2a in the medaka and three-spined stickleback genomes.
authors: Department of Biology, Dalhousie University, 1355 Oxford Street, P.O. Box 15000, Halifax, NS B3H 4R2, Canada.
authors: Manoj B
authors: MB
authors: Parmar
authors: Jonathan M
authors: JM
authors: Wright
mesh_terms: D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020440:Gene Duplication; D016678:Genome; D023281:Genomics; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*; D010802:Phylogeny; D023701:Smegmamorpha / Q000235:genetics*; D026801:Synteny; D014158:Transcription, Genetic; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C497193:FABP2 protein, zebrafish; D050556:Fatty Acid-Binding Proteins; D029961:Zebrafish Proteins