title: Oil, fatty acid, flavonoid, and resveratrol content variability and FAD2A functional SNP genotypes in the U.S. peanut mini-core collection.
abstract: Peanut seeds contain high amounts of oil and protein as well as some useful bioactive phytochemicals which can contribute to human health. The U.S. peanut mini-core collection is an important genetic resource for improving seed quality and developing new cultivars. Variability of seed chemical composition within the mini-core was evaluated from freshly harvested seeds for two years. Oil, fatty acid composition, and flavonoid/resveratrol content were quantified by NMR, GC, and HPLC, respectively. Significant variability was detected in seed chemical composition among accessions and botanical varieties. Accessions were further genotyped with a functional SNP marker from the FAD2A gene using real-time PCR and classified into three genotypes with significantly different O/L ratios: wild type (G/G with a low O/L ratio <1.7), heterozygote (G/A with O/L ratio >1.4 but <1.7), and mutant (A/A with a high O/L ratio >1.7). The results from real-time PCR genotyping and GC fatty acid analysis were consistent. Accessions with high amounts of oil, quercetin, high seed weight, and O/L ratio were identified. The results from this study may be useful not only for peanut breeders, food processors, and product consumers to select suitable accessions or cultivars but also for curators to potentially expand the mini-core collection.
authors: USDA-ARS , Plant Genetic Resources Conservation Unit, Griffin, Georgia 30223, United States.
authors: Ming Li
authors: ML
authors: Wang
authors: Charles Y
authors: CY
authors: Chen
authors: Brandon
authors: B
authors: Tonnis
authors: Noelle A
authors: NA
authors: Barkley
authors: David L
authors: DL
authors: Pinnow
authors: Roy N
authors: RN
authors: Pittman
authors: Jerry
authors: J
authors: Davis
authors: C Corley
authors: CC
authors: Holbrook
authors: H Thomas
authors: HT
authors: Stalker
authors: Gary A
authors: GA
authors: Pederson
mesh_terms: D010367:Arachis / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis* / Q000378:metabolism; D005838:Genotype; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D010938:Plant Oils / Q000032:analysis* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D000077185:Resveratrol; D012639:Seeds / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D013267:Stilbenes / Q000032:analysis* / Q000378:metabolism; D014481:United States
chemical_list: D005227:Fatty Acids; D005419:Flavonoids; D010936:Plant Extracts; D010938:Plant Oils; D013267:Stilbenes; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase; D000077185:Resveratrol