title: Persistence of mixed staphylococci assemblages following disinfection of hospital room surfaces.
abstract: The distribution of staphylococcal assemblages on surfaces in hospital rooms was assessed before and after daily disinfection with quaternary ammonia products. DNA was extracted from enrichment cultures of bacteria, which were swabbed from each of nine surface types, and subjected to analysis by staphylococci-specific, denaturing gradient gel electrophoresis. A genetic marker for Staphylococcus epidermidis/kloosii was detected on all surface types before and after cleaning, whereas markers for Staphylococcus aureus and Staphylococcus lugdunensis were detected on five surface types. Overall, genetic makers for several staphylococci known to colonize and infect humans remained ubiquitous in each room following daily disinfection practices.
authors: Department of Environmental Sciences, University of Toledo, Toledo, OH, USA. von.sigler@utoledo.edu
authors: V
authors: V
authors: Sigler
authors: S
authors: S
authors: Hensley
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D004783:Environmental Microbiology; D006302:Health Services Research; D006761:Hospitals; D006801:Humans; D010362:Patients' Rooms; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D018570:Risk Assessment; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D057787:Staphylococcus lugdunensis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004202:Disinfectants; D000644:Quaternary Ammonium Compounds