title: The 16p13.3 (PDPK1) Genomic Gain in Prostate Cancer: A Potential Role in Disease Progression.
abstract: BACKGROUND
  Prostate cancer (PCa) is a leading cause of cancer death, and distinguishing aggressive from indolent tumors is a major challenge. Identification and characterization of genomic alterations associated with advanced disease can provide new markers of progression and better therapeutic approaches.
  
  
  METHODS
  We performed fluorescence in situ hybridization to detect the copy number gain of chromosome 16p13.3 in 75 PCa samples including 10 lymph node (LN) metastases and their matched primary tumors, 9 samples of castration-resistant prostate cancer (CRPC), and 46 additional primary PCa specimens with clinicopathologic parameters.
  
  
  RESULTS
  We detected the gain in 5 of 10 LN metastases and 3 of 5 matched primary tumors, 3 of 9 CRPC samples, and 9 of 46 (20%) primary tumors where the 16p13.3 alteration was associated with high Gleason score and elevated preoperative prostate-specific antigen levels. The level of 16p13.3 gain was higher in LN metastasis and CRPC specimens compared to primary PCa. Chromosome mapping revealed the gain spans PDPK1 encoding the 3-phosphoinositide-dependent protein kinase-1 (PDK1). Knockdown of PDK1 in three PCa cell lines reduced migration without affecting growth and re-expressing PDK1 rescued motility.
  
  
  CONCLUSIONS
  Our findings support a prognostic value of the 16p13.3 gain and a role of PDK1 in PCa progression through migration.
authors: Department of Surgery, Division of Urology, McGill University and the Research Institute of the McGill University Health Centre (RI MUHC), Montreal, Quebec, Canada.
authors: Khalil A
authors: KA
authors: Choucair
authors: Karl-Philippe
authors: KP
authors: Guérard
authors: Joshua
authors: J
authors: Ejdelman
authors: Simone
authors: S
authors: Chevalier
authors: Maisa
authors: M
authors: Yoshimoto
authors: Eleonora
authors: E
authors: Scarlata
authors: Ladan
authors: L
authors: Fazli
authors: Kanishka
authors: K
authors: Sircar
authors: Jeremy A
authors: JA
authors: Squire
authors: Fadi
authors: F
authors: Brimo
authors: Isabela W
authors: IW
authors: Cunha
authors: Armen
authors: A
authors: Aprikian
authors: Martin
authors: M
authors: Gleave
authors: Jacques
authors: J
authors: Lapointe
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