title: [Expressions of SOCS   in   chondrocytes of patients with   osteoarthritis].
abstract: OBJECTIVE
  To study the gene expressions of suppressors of cytokine signaling (SOCS1, 2, 3) and cytokine-inducible SH2-domain 1(CIS-1) in chondrocytes of osteoarthritis (OA) patients and healthy controls, and also analyze the effects of IL-1β and TNF-α on the levels of mRNA encoding these SOCS family members.
  
  
  METHODS
  Chondrocytes were isolated from patients of OA and joint replacement due to traffic accident by sequential treatment with trypsin, hyaluronidase and collagenase B. Total RNA was extracted from the same chondrocytes, and the levels of SOCS1, 2, 3 and CIS-1 mRNA were determined by real-time qRT-PCR. In addition, healthy chondrocytes were cultured with and without a mixture of IL-1β and oncostatin M (OSM, both 2.5 ng/mL) or TNF-α (10 ng/mL). The short-term cultures with single cytokine treatment were harvested 24 and 72 h after treatment, and the long-term cultures were maintained for 4-5 weeks until confluent with periodical cytokine stimulation. Total RNA was extracted and mRNA levels of SOCS1, 2, 3 and CIS-1 mRNA were detected by qRT-PCR.
  
  
  RESULTS
  The SOCS2 and CIS-1 mRNA levels were reduced by approximately 8-fold in OA samples compared to controls (P<0.01), whereas SOCS1 and SOCS3 showed similar expression patterns in OA and control chondrocytes (P>0.05). The SOCS2 and CIS-1 mRNA levels declined by 5-fold and 3-fold with long-term treatment with IL-1β and OSM or IL-1β and TNF-α, respectively (P<0.05).
  
  
  CONCLUSIONS
  In OA patients, the expressions of SOCS2 and CIS-1 decreased, while SOCS1 and SOCS3 were unaffected. Long-term treatment with inflammatory cytokines mimicking OA effect attenuated the expressions of SOCS2 and CIS-1 in chondrocytes.
authors: Department of Orthopedics, First Affiliated Hospital, Xinxiang Medical College, Weihui, China. sunxiaohui123789@163.com
authors: Xiaohui
authors: X
authors: Sun
mesh_terms: D019902:Chondrocytes / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D010003:Osteoarthritis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C108944:SOCS1 protein, human; C106643:SOCS2 protein, human; C106644:SOCS3 protein, human