title: Completion of cell division is associated with maximum telomerase activity in naturally synchronized cultures of the green alga Desmodesmus quadricauda.
abstract: Telomerase maintains the ends of eukaryotic chromosomes, and its activity is an important parameter correlating with the proliferative capacity of cells. We have investigated cell cycle-specific changes in telomerase activity using cultures of Desmodesmus quadricauda, a model alga naturally synchronized by light/dark entrainment. A quantitative telomerase assay revealed high activity in algal cultures, with slight changes during the light period. Significantly increased telomerase activity was observed at the end of the dark phase, when cell division was complete. In contrast to other models, a natural separation between nuclear and cellular division typical for the cell cycle in D. quadricauda made this observation possible.
authors: Institute of Biophysics, Academy of Sciences of the Czech Republic, vvi, 612 65 Brno, Czech Republic.
authors: Tereza
authors: T
authors: Ševčíková
authors: Kateřina
authors: K
authors: Bišová
authors: Miloslava
authors: M
authors: Fojtová
authors: Alena
authors: A
authors: Lukešová
authors: Kristýna
authors: K
authors: Hrčková
authors: Eva
authors: E
authors: Sýkorová
mesh_terms: D001681:Biological Assay; D002455:Cell Division; D002478:Cells, Cultured; D000460:Chlorophyta / Q000166:cytology / Q000201:enzymology* / Q000528:radiation effects; D007700:Kinetics; D008027:Light; D017440:Photoperiod; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D019098:Telomerase / Q000302:isolation & purification / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D010940:Plant Proteins; D019098:Telomerase