title: Identification of polyphenols and their metabolites in human urine after cranberry-syrup consumption.
abstract: As the beneficial effects of American cranberry (Vaccinium macrocarpon) can be partly attributed to its phenolic composition, the evaluation of the physiological behaviour of this fraction is crucial. A rapid and sensitive method by ultra-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been used to identify phenolic metabolites in human urine after a single dose of cranberry syrup. Prior to the analysis, metabolites were extracted using an optimised solid-phase extraction procedure. All possible metabolites were investigated based on retention time, accurate mass data and isotope and fragmentation patterns. Free coumaroyl hexose (isomer 1 and 2), dihydroxybenzoic acid, caffeoyl glucose, dihydroferulic acid 4-O-β-d-glucuronide, methoxyquercetin 3-O-galactoside, scopoletin, myricetin and quercetin, together with other 23 phase-I and phase-II metabolites, including various isomers, could be tentatively identified in the urine. Afterwards, the metabolites were simultaneously screened in the urine of different subjects at 0, 2, 4, and 6h after the ingestion of cranberry syrup by Target Analysis(TM) software.
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain.
authors: Ihsan
authors: I
authors: Iswaldi
authors: David
authors: D
authors: Arráez-Román
authors: Ana María
authors: AM
authors: Gómez-Caravaca
authors: María Del Mar
authors: Mdel M
authors: Contreras
authors: José
authors: J
authors: Uberos
authors: Antonio
authors: A
authors: Segura-Carretero
authors: Alberto
authors: A
authors: Fernández-Gutiérrez
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D059808:Polyphenols / Q000032:analysis* / Q000652:urine; D029799:Vaccinium macrocarpon
chemical_list: D059808:Polyphenols