title: Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform.
abstract: OBJECTIVE
  The design of novel F3-targeted liposomes with adequate features for systemic administration, to enable efficient intracellular delivery of siRNA toward both cancer and endothelial cells from angiogenic blood vessels.
  
  
  METHODS
  Cellular association studies were performed by flow cytometry. Gene silencing was evaluated with eGFP-overexpressing cells, by flow cytometry and real-time reverse-transcription PCR. Safety and immunogenicity was assessed in CD1 mice.
  
  
  RESULTS
  A strong improvement on siRNA internalization by the target cells was achieved, which was correlated with effective downregulation of eGFP. In addition, the F3-targeted liposomes were nonimmunogenic, even in a multiadministration schedule.
  
  
  CONCLUSIONS
  Overall, the developed F3-targeted nanocarrier constitutes a valuable tool for the specific and safe systemic delivery of siRNA to solid tumors.
authors: Center for Neurosciences & Cell Biology (CNC), University of Coimbra, Portugal.
authors: Lígia C
authors: LC
authors: Gomes-da-Silva
authors: Yolanda
authors: Y
authors: Fernández
authors: Ibane
authors: I
authors: Abasolo
authors: Simo
authors: S
authors: Schwartz
authors: José S
authors: JS
authors: Ramalho
authors: Maria C
authors: MC
authors: Pedroso de Lima
authors: Sérgio
authors: S
authors: Simões
authors: João N
authors: JN
authors: Moreira
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D042783:Endothelial Cells / Q000378:metabolism; D005434:Flow Cytometry; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009369:Neoplasms / Q000235:genetics* / Q000628:therapy; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics
chemical_list: D008081:Liposomes; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins