title: Circulating human basophils lack the features of professional antigen presenting cells.
abstract: Recent reports in mice demonstrate that basophils function as antigen presenting cells (APC). They express MHC class II and co-stimulatory molecules CD80 and CD86, capture and present soluble antigens or IgE-antigen complexes and polarize Th2 responses. Therefore, we explored whether human circulating basophils possess the features of professional APC. We found that unlike dendritic cells (DC) and monocytes, steady-state circulating human basophils did not express HLA-DR and co-stimulatory molecules CD80 and CD86. Basophils remained negative for these molecules following stimulation with soluble Asp f 1, one of the allergens of Aspergillus fumigatus; Bet v 1, the major birch allergen; TLR2-ligand or even upon IgE cross-linking. Unlike DC, Asp f 1-pulsed basophils did not promote Th2 responses as analyzed by the secretion of IL-4 in the basophil-CD4(+) T cell co-culture. Together, these results demonstrate the inability of circulating human basophils to function as professional APC.
authors: Unité 872, Institut National de la Santé et de la Recherche Médicale, Paris, F-75006, France.
authors: Meenu
authors: M
authors: Sharma
authors: Pushpa
authors: P
authors: Hegde
authors: Vishukumar
authors: V
authors: Aimanianda
authors: Remi
authors: R
authors: Beau
authors: Mohan S
authors: MS
authors: Maddur
authors: Helene
authors: H
authors: Sénéchal
authors: Pascal
authors: P
authors: Poncet
authors: Jean-Paul
authors: JP
authors: Latgé
authors: Srini V
authors: SV
authors: Kaveri
authors: Jagadeesh
authors: J
authors: Bayry
mesh_terms: D000485:Allergens / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D052179:Antigens, Plant / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D001491:Basophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
chemical_list: C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D052179:Antigens, Plant; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D005656:Fungal Proteins; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; C061312:Bet v 1 allergen, Betula; D015847:Interleukin-4
references: Respir Res. 2011 Sep 21;12:122
references: 21936897
references: Nature. 1998 Mar 19;392(6673):245-52
references: 9521319
references: Immunity. 2005 Aug;23(2):191-202
references: 16111637
references: J Allergy Clin Immunol. 2000 Jun;105(6 Pt 1):1185-93
references: 10856154
references: J Immunol. 2011 Jun 1;186(11):6083-90
references: 21597041
references: Nat Med. 2010 Jun;16(6):701-7
references: 20512127
references: Nat Immunol. 2009 Jul;10(7):706-12
references: 19465908
references: J Immunol. 2011 Mar 1;186(5):2719-28
references: 21270410
references: Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2586-91
references: 20133791
references: Annu Rev Immunol. 2005;23:975-1028
references: 15771591
references: Curr Protoc Immunol. 2001 May;Chapter 7:Unit 7.24
references: 18432835
references: PLoS Pathog. 2012;8(5):e1002700
references: 22693445
references: Clin Vaccine Immunol. 2009 Oct;16(10):1485-92
references: 19675222
references: Nat Immunol. 2009 Jul;10(7):697-705
references: 19465906
references: J Immunol. 2009 Aug 15;183(4):2407-14
references: 19625642
references: Trends Immunol. 2010 Feb;31(2):45-8
references: 20060781
references: Curr Allergy Asthma Rep. 2003 Sep;3(5):430-7
references: 12906782
references: Nature. 2009 Aug 27;460(7259):1117-21
references: 19713928
references: Clin Exp Allergy. 2000 Mar;30(3):359-69
references: 10691894
references: Inflamm Res. 2005 Apr;54 Suppl 1:S13-4
references: 15928814
references: Blood. 2012 Apr 19;119(16):3863-4
references: 22517877
references: Blood. 2009 Mar 19;113(12):2816-25
references: 18941115
references: Diagn Microbiol Infect Dis. 2006 Aug;55(4):279-91
references: 16626916
references: Mucosal Immunol. 2010 Mar;3(2):129-37
references: 20072123
references: Med Mycol. 2005 May;43 Suppl 1:S189-96
references: 16110811
references: Mucosal Immunol. 2008 Mar;1(2):156-68
references: 19079173
references: Annu Rev Immunol. 2011;29:45-69
references: 21166539
references: Allergy. 2012 May;67(5):593-600
references: 22188598
references: Nature. 2000 Sep 14;407(6801):258-64
references: 11001069
references: J Allergy Clin Immunol. 2004 Nov;114(5):997-1008; quiz 1009
references: 15536399
references: Allergy. 2012 May;67(5):601-8
references: 22335568
references: Nat Immunol. 2008 Jul;9(7):733-42
references: 18516038
references: N Engl J Med. 2010 Sep 9;363(11):1080-2
references: 20825323
references: Clin Microbiol Rev. 1999 Apr;12(2):310-50
references: 10194462
references: Nat Immunol. 2009 Jul;10(7):713-20
references: 19465907
references: Physiol Rev. 2004 Apr;84(2):579-621
references: 15044683
references: J Allergy Clin Immunol. 2005 Feb;115(2):295-301
references: 15696084
references: J Allergy Clin Immunol. 2012 Apr;129(4):889-901
references: 22464668