title: Analysis of microbial population dynamics in a partial nitrifying SBR at ambient temperature.
abstract: In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study microbial population dynamics. Partial nitrification was effectively achieved in response to shifts of influent ammonium concentrations. DGGE results showed that higher ammonia concentration referred to lower ammonia-oxidizing bacteria (AOB) diversity in the SBR. Phylogenetic analysis revealed that all the predominant AOB was affiliated with Nitrosomonas genus. FISH analysis illustrated AOB was the predominant nitrifying bacteria of microbial compositions when SBR achieved partial nitrification (PN) at ambient temperature.
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China. biowater@126.com
authors: Taotao
authors: T
authors: Zeng
authors: Dong
authors: D
authors: Li
authors: Huiping
authors: H
authors: Zeng
authors: Zhao
authors: Z
authors: Zhang
authors: Liqian
authors: L
authors: Liu
authors: Xiaojing
authors: X
authors: Zhang
authors: Jie
authors: J
authors: Zhang
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001483:Base Sequence; D019149:Bioreactors; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D059013:Microbial Consortia; D058465:Nitrification; D009606:Nitrosomonas / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification
chemical_list: D000641:Ammonia; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
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