title: Protein adsorption on single-crystal hydroxyapatite particles with preferred orientation to a(b)- and c-axes.
abstract: Adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) proteins with preferred orientation to a(b)- and c-axes of single-crystal hydroxyapatite (HAp) particles, was investigated. Fiber-like HAp single crystal particles (aHAp) and plate-like HAp single crystal particles (cHAp) were used as models for a(b)-plane and c-plane oriented HAps, respectively, together with randomly shaped HAp particles (iHAp) as a control. The selective adsorption behaviors of negatively charged BSA and positively charged LSZ on these HAp particles were examined in a phosphate buffered saline at pH 7.3 and 25 °C for 48 h. The amount of BSA adsorption, normalized for specific surface area, was in the order of aHAp > iHAp > cHAp; however, the order for LSZ was reversed as cHAp > iHAp ≒ aHAp. These results indicate that the a(b)- and c-planes of HAp crystal have high specificity for the adsorption of acidic or basic proteins.
authors: Department of Applied Chemistry, School of Science and Technology, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
authors: Zhi
authors: Z
authors: Zhuang
authors: Mamoru
authors: M
authors: Aizawa
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D003460:Crystallization / Q000379:methods; D017886:Durapatite / Q000737:chemistry*; D008968:Molecular Conformation; D057139:Nanofibers / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013237:Stereoisomerism; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D012710:Serum Albumin, Bovine; D017886:Durapatite
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