title: Gender difference of serum angiotensin-converting enzyme (ACE) activity in DD genotype of ACE insertion/deletion polymorphism in elderly Chinese.
abstract: BACKGROUND
  In this study we investigated the gender difference of serum angiotensin-converting enzyme (ACE) activity in a population of Hong Kong-dwelling elderly Chinese.
  
  
  METHODS
  A total of 1767 (843 male, 924 female) Hong Kong-dwelling elderly Chinese were recruited. ACE I/D genotypes were identified by polymerase chain reaction amplification and serum ACE activity was determined using a commercially available kinetic kit. ACE I/D genotype distribution was compared by chi-square test, the correlation between ACE I/D polymorphism and serum ACE activity was analysed by ANOVA test and gender difference of serum ACE activity of different genotypes was compared by independent sample t-test.
  
  
  RESULTS
  No statistically significant difference of genotype distribution between male and female subjects was found. Serum ACE activity was significantly correlated with ACE genotype. Overall, there was no gender difference of serum ACE activity; however, when sub-grouping the subjects by ACE I/D genotype, male subjects with DD genotype had higher serum ACE activity than female subjects with DD genotype.
  
  
  CONCLUSIONS
  No significant gender difference of genotype distribution was found in elderly Chinese. Serum ACE activity was significantly correlated with ACE I/D polymorphism in elderly Chinese. Male subjects with DD genotype had higher serum ACE activity than female subjects with DD genotype.
authors: Department of Orthopaedics, The Affiliated Hospital to Nantong University, PR China Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors: Ya-Feng
authors: YF
authors: Zhang
authors: Jiangsu Provincial Key Laboratory of Neural Regeneration, Nantong University, PR China.
authors: Qiong
authors: Q
authors: Cheng
authors: Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors: Nelson L S
authors: NL
authors: Tang
authors: Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors: Tanya T W
authors: TT
authors: Chu
authors: Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors: Brian
authors: B
authors: Tomlinson
authors: Department of Orthopaedics, The Affiliated Hospital to Nantong University, PR China.
authors: Fan
authors: F
authors: Liu
authors: Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China tkwok@cuhk.edu.hk.
authors: Timothy C Y
authors: TC
authors: Kwok
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D002681:China; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000097:blood* / Q000235:genetics*; D011110:Polymorphism, Genetic; D012727:Sex Characteristics
chemical_list: D007703:Peptidyl-Dipeptidase A
keywords: Angiotensin-converting enzyme; gender difference; insertion/deletion polymorphism