title: Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton.
abstract: Digging up skeletons: We report the identification and the functional characterization of two terpene cyclases (DtcycA and DtcycB) that were mined from the genome of Streptomyces sp. SANK 60404. DtcycA and DtcycB are novel bacterial diterpene cyclases for the synthesis of the cembrane skeleton.
authors: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors: Ayuko
authors: A
authors: Meguro
authors: Takeo
authors: T
authors: Tomita
authors: Makoto
authors: M
authors: Nishiyama
authors: Tomohisa
authors: T
authors: Kuzuyama
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003500:Cyclization; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D016680:Genome, Bacterial; D007700:Kinetics; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004224:Diterpenes; C037071:cembrane; D019883:Alkyl and Aryl Transferases
references: J Bacteriol. 2006 Feb;188(4):1236-44
references: 16452404
references: J Antibiot (Tokyo). 2004 Nov;57(11):739-47
references: 15712669
references: FEBS Lett. 2009 Jan 22;583(2):475-80
references: 19121310
references: Nat Prod Rep. 2012 Oct;29(10):1153-75
references: 22907771
references: Nat Chem Biol. 2011 May 22;7(7):431-3
references: 21602811
references: J Antibiot (Tokyo). 2008 Sep;61(9):545-9
references: 19160521
references: Chem Commun (Camb). 2005 Feb 28;(8):1016-8
references: 15719101
references: FEMS Microbiol Lett. 1999 May 15;174(2):251-3
references: 10339816
references: J Biol Chem. 2002 Oct 4;277(40):37098-104
references: 12138123
references: J Antibiot (Tokyo). 2008 Sep;61(9):537-44
references: 19160520
references: Plant Mol Biol. 2010 Aug;73(6):673-85
references: 20495852
references: Biochemistry. 2000 Feb 8;39(5):890-902
references: 10653632
references: Methods Enzymol. 1985;110:130-44
references: 2991702
references: Science. 1997 Sep 19;277(5333):1788-9
references: 9324768
references: J Mol Biol. 1990 Oct 5;215(3):403-10
references: 2231712
references: Biosci Biotechnol Biochem. 2011;75(1):75-81
references: 21228491
references: Chembiochem. 2012 Jan 23;13(2):202-14
references: 22213220
references: Curr Opin Chem Biol. 2012 Apr;16(1-2):132-41
references: 22445175
references: Chem Rev. 2006 Aug;106(8):3412-42
references: 16895335
references: J Bacteriol. 2001 Oct;183(20):6085-94
references: 11567009
references: Chem Biol. 2009 Jul 31;16(7):736-43
references: 19635410
references: Phytochemistry. 2010 Sep;71(13):1466-73
references: 20594566
references: Genome Res. 2008 May;18(5):802-9
references: 18332092
references: Chembiochem. 2007 May 25;8(8):869-74
references: 17457817
references: Nat Chem Biol. 2007 Jul;3(7):396-407
references: 17576427
references: Nature. 2011 Jan 6;469(7328):116-20
references: 21160477