title: SecYEG activates GTPases to drive the completion of cotranslational protein targeting.
abstract: Signal recognition particle (SRP) and its receptor (SR) comprise a highly conserved cellular machine that cotranslationally targets proteins to a protein-conducting channel, the bacterial SecYEG or eukaryotic Sec61p complex, at the target membrane. Whether SecYEG is a passive recipient of the translating ribosome or actively regulates this targeting machinery remains unclear. Here we show that SecYEG drives conformational changes in the cargo-loaded SRP-SR targeting complex that activate it for GTP hydrolysis and for handover of the translating ribosome. These results provide the first evidence that SecYEG actively drives the efficient delivery and unloading of translating ribosomes at the target membrane.
authors: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors: David
authors: D
authors: Akopian
authors: Kush
authors: K
authors: Dalal
authors: Kuang
authors: K
authors: Shen
authors: Franck
authors: F
authors: Duong
authors: Shu-ou
authors: SO
authors: Shan
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000502:physiology*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D008954:Models, Biological; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D018000:Receptors, Peptide / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D012270:Ribosomes / Q000378:metabolism / Q000502:physiology; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*
chemical_list: D029968:Escherichia coli Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle; C036318:signal peptide receptor; D006160:Guanosine Triphosphate
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