title: miR-143 inhibits glycolysis and depletes stemness of glioblastoma stem-like cells.
abstract: Glioblastomas rely mainly on aerobic glycolysis to sustain proliferation and growth; however, little is known about the regulatory mechanisms of metabolism in glioblastoma stem cells. We show that miR-143 is significantly down-regulated in glioma tissues and glioblastoma stem-like cells (GSLCs), while miR-143 over-expression inhibits glycolysis by directly targeting hexokinase 2, and promotes differentiation of GSLCs. Moreover, miR-143 inhibits proliferation of GSLCs under hypoxic conditions and decreases tumor formation capacity of GSLCs in vivo. We also show that a combination of miR-143 and 2-DG, a widely used glycolysis inhibitor, has synergistic effects against GSLCs. miR-143 is a potential therapeutic target for glioblastoma treatment.
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, People's Republic of China. guangsz@hotmail.com
authors: Shiguang
authors: S
authors: Zhao
authors: Huailei
authors: H
authors: Liu
authors: Yaohua
authors: Y
authors: Liu
authors: Jianing
authors: J
authors: Wu
authors: Chunlei
authors: C
authors: Wang
authors: Xu
authors: X
authors: Hou
authors: Xiaofeng
authors: X
authors: Chen
authors: Guang
authors: G
authors: Yang
authors: Ling
authors: L
authors: Zhao
authors: Hui
authors: H
authors: Che
authors: Yunke
authors: Y
authors: Bi
authors: Hongyu
authors: H
authors: Wang
authors: Fei
authors: F
authors: Peng
authors: Jing
authors: J
authors: Ai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D015687:Cell Hypoxia / Q000235:genetics; D045744:Cell Line, Tumor; D003847:Deoxyglucose / Q000494:pharmacology; D005260:Female; D005909:Glioblastoma / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology*; D051381:Rats
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D003847:Deoxyglucose