title: Understanding the cellulolytic system of Trichoderma harzianum P49P11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-L-arabinofuranosidase.
abstract: Supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. This study investigated which enzymes were complementary to the enzyme set of Trichoderma harzianum in the degradation of sugarcane bagasse. Specific activities of T. harzianum extract on different substrates were compared with the extracts of Penicillium echinulatum and Trichoderma reesei, and two commercial cellulase preparations. Complementary analysis of the secretome of T. harzianum was also used to identify which enzymes were produced during growth on pretreated sugarcane bagasse. These analyses enabled the selection of the enzymes pectinase and α-L-arabinofuranosidase (AF) to be further investigated as supplements to the T. harzianum extract. The effect of enzyme supplementation on the efficiency of sugarcane bagasse saccharification was evaluated using response surface methodology. The supplementation of T. harzianum enzymatic extract with pectinase and AF increased the efficiency of hydrolysis by up to 116%.
authors: Brazilian Bioethanol Science and Technology Laboratory - CTBE, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas, São Paulo, Brazil. priscila.delabona@bioetanol.org.br
authors: Priscila da Silva
authors: Pda S
authors: Delabona
authors: Júnio
authors: J
authors: Cota
authors: Zaira Bruna
authors: ZB
authors: Hoffmam
authors: Douglas Antonio Alvaredo
authors: DA
authors: Paixão
authors: Cristiane Sanchez
authors: CS
authors: Farinas
authors: João Paulo Lourenço Franco
authors: JP
authors: Cairo
authors: Deise Juliana
authors: DJ
authors: Lima
authors: Fábio Marcio
authors: FM
authors: Squina
authors: Roberto
authors: R
authors: Ruller
authors: José Geraldo da Cruz
authors: JG
authors: Pradella
mesh_terms: D002241:Carbohydrates / Q000096:biosynthesis* / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006868:Hydrolysis; D011096:Polygalacturonase / Q000737:chemistry*; D031786:Saccharum / Q000382:microbiology*; D014242:Trichoderma / Q000737:chemistry / Q000145:classification / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D002482:Cellulose; C027433:bagasse; D006026:Glycoside Hydrolases; D011096:Polygalacturonase; C019713:alpha-N-arabinofuranosidase