title: Biochemical properties of Hemigraphis alternata incorporated chitosan hydrogel scaffold.
abstract: In this work, Hemigraphis alternata extract incorporated chitosan scaffold was synthesized and characterized for wound healing. The antibacterial activity of Hemigraphis incorporated chitosan scaffold (HIC) against Escherichia coli and Staphylococcus aureus was evaluated which showed a reduction in total colony forming units by 45-folds toward E. coli and 25-fold against S. aureus respectively. Cell viability studies using Human Dermal Fibroblast cells (HDF) showed 90% viability even at 48 h when compared to the chitosan control. The herbal scaffold made from chitosan was highly haemostatic and antibacterial. The obtained results were in support that the herbal scaffold can be effectively applied for infectious wounds.
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682 041, India.
authors: M
authors: M
authors: Annapoorna
authors: P T
authors: PT
authors: Sudheesh Kumar
authors: Lakshmi R
authors: LR
authors: Lakshman
authors: Vinoth-Kumar
authors: VK
authors: Lakshmanan
authors: Shantikumar V
authors: SV
authors: Nair
authors: R
authors: R
authors: Jayakumar
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D015539:Platelet Activation / Q000187:drug effects; D016062:Porosity; D013211:Staphylococcus aureus / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D020100:Hydrogels; D010936:Plant Extracts; D014867:Water; D048271:Chitosan