title: [Experimental study on effect of different chromatographic conditions on QAMS relative correlation factors and relative retention values].
abstract: OBJECTIVE
  To investigate the effect of different chromatographic conditions on QAMS relative correlation factors (RCF) and relative retention values (RT(R)).
  
  
  METHODS
  C18 columns were used with methyl alcohol-0.4% phosphoric acid water (85: 15) as the mobile phase. The detection wavelength was 254 nm, the column temperature was 30 degrees C, and the flow rate was 1.0 mL x min(-1). The five anthraquinones in Rhei Radix et Rhizoma were selected to be the objects of study. The RCF and RT(R) among aloe-emodin, rhein, chrysophanol, physcion and emodin were determined under different chromatographic conditions.
  
  
  RESULTS
  Their RCFs showed no significant difference.
  
  
  CONCLUSIONS
  The RCFs among anthraquinones established by QAMS can be used as a constant in content determination of traditional Chinese medicines/patent traditional Chinese medicines.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. weihong_bj@126.com
authors: Wei-Hong
authors: WH
authors: Feng
authors: Fei
authors: F
authors: Yang
authors: Zhi-Min
authors: ZM
authors: Wang
authors: Qi-Wei
authors: QW
authors: Zhang
authors: Dong-Ying
authors: DY
authors: Li
authors: Li-Na
authors: LN
authors: Ji
authors: Yu-Ying
authors: YY
authors: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012250:Rheum / Q000737:chemistry*