title: Assessment of adiponectin and its isoforms in Polish centenarians.
abstract: BACKGROUND
  The physiological mechanisms that promote longevity remain unclear. It has been suggested that insulin sensitivity is preserved in centenarians, whereas typical aging is accompanied by increasing insulin resistance. The oldest-old individuals display raised total adiponectin levels, despite the potential correlation between enhanced adiponectin and all-cause and cardiovascular mortality.
  
  
  OBJECTIVE
  To evaluate the level of adiponectin and its isoforms in sera of centenarians and to assess associations between adiponectin and metabolic parameters.
  
  
  METHODS
  A group of 58 Polish centenarians (50 women and 8 men, mean age 101±1.34 years) and 68 elderly persons (55 women and 13 men, mean age 70±5.69 years) as controls.
  
  
  METHODS
  Serum samples were analyzed to evaluate the following parameters: adiponectin array (total adiponectin, HWM-, MMW- and LMW-adiponectin; all by ELISA methods), insulin (by IRMA methods), glucose and lipid profiles. HOMA-IR was calculated. Clinical data were collected. Statistical analyses were performed.
  
  
  RESULTS
  The concentrations of all adiponectin isoforms were significantly higher in the oldest-old participants. In the centenarian group, total adiponectin positively correlated with age and HDL-cholesterol, and HMW-adiponectin was negatively associated with insulin and triglycerides. The long-lived participants had a lower incidence of hypertension, type 2 diabetes, overweight and obesity, with lower concentrations of serum glucose and insulin, and reduced HOMA-IR.
  
  
  CONCLUSIONS
  Our findings support the thesis that centenarians possess a different adiponectin isoform pattern and have a favorable metabolic phenotype in comparison with elderly individuals. However, additional work is necessary to understand the relevance of these findings to longevity.
authors: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland. wojtmed@wp.pl
authors: Wojciech
authors: W
authors: Bik
authors: Agnieszka
authors: A
authors: Baranowska-Bik
authors: Ewa
authors: E
authors: Wolinska-Witort
authors: Malgorzata
authors: M
authors: Kalisz
authors: Katarzyna
authors: K
authors: Broczek
authors: Malgorzata
authors: M
authors: Mossakowska
authors: Boguslawa
authors: B
authors: Baranowska
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D008076:Cholesterol, HDL / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism; D005260:Female; D015577:Geriatric Assessment / Q000379:methods; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D008970:Molecular Weight; D009765:Obesity / Q000097:blood / Q000378:metabolism; D020033:Protein Isoforms / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D012044:Regression Analysis; D013223:Statistics as Topic; D014280:Triglycerides / Q000097:blood
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D008076:Cholesterol, HDL; D007328:Insulin; D020033:Protein Isoforms; D014280:Triglycerides