title: Pre-storage of gelified reagents in a lab-on-a-foil system for rapid nucleic acid analysis.
abstract: Reagent pre-storage in a microfluidic chip can enhance operator convenience, simplify the system design, reduce the cost of storage and shipment, and avoid the risk of cross-contamination. Although dry reagents have long been used in lateral flow immunoassays, they have rarely been used for nucleic acid-based point-of-care (POC) assays due to the lack of reliable techniques to dehydrate and store fragile molecules involved in the reaction. In this study, we describe a simple and efficient method for prolonged on-chip storage of PCR reagents. The method is based on gelification of all reagents required for PCR as a ready-to-use product. The approach was successfully implemented in a lab-on-a-foil system, and the gelification process was automated for mass production. Integration of reagents on-chip by gelification greatly facilitated the development of easy-to-use lab-on-a-chip (LOC) devices for fast and cost-effective POC analysis.
authors: DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs. Lyngby, Denmark.
authors: Yi
authors: Y
authors: Sun
authors: Jonas
authors: J
authors: Høgberg
authors: Thanner
authors: T
authors: Christine
authors: Laouenan
authors: L
authors: Florian
authors: Lisandro G
authors: LG
authors: Monsalve
authors: Sonia
authors: S
authors: Rodriguez
authors: Cuong
authors: C
authors: Cao
authors: Anders
authors: A
authors: Wolff
authors: Jesus M
authors: JM
authors: Ruano-Lopez
authors: Dang Duong
authors: DD
authors: Bang
mesh_terms: D016123:Campylobacter jejuni / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial