title: Aberrant lymphocyte enhancer-binding factor 1 expression is characteristic for sporadic Burkitt's lymphoma.
abstract: Burkitt's lymphoma (BL) is a highly malignant, aggressive non-Hodgkin's lymphoma derived from germinal center B cells. Recently, global gene expression profiling of patient samples led to a molecular definition of BL with lymphocyte enhancer-binding factor 1 (LEF1) as a signature gene. Herein, we report the expression of nucleic LEF1 in 15 of 18 patients with BL and the identification of LEF1 target genes. Germinal center B cells were devoid of detectable nuclear LEF1 expression, as were mantle cell lymphoma (0 of 5), marginal zone lymphoma (0 of 6), follicular lymphoma (0 of 12), and diffuse large B-cell lymphoma (1 of 31). Whole-genome gene expression profiling after transient knockdown of LEF1 in BL cell lines identified new LEF1 target genes; these LEF1 targets are enriched with genes associated with cancers. The expression of LEF1 and LEF1-regulated genes in primary BL suggests that LEF1 is not only aberrantly expressed but also transcriptionally active. This study supports a functionally important role for LEF1 and its target genes in BLs.
authors: Department of Hematology and Oncology, University Medical Centre of the Georg-August University of Göttingen, Göttingen, Germany.
authors: Neele
authors: N
authors: Walther
authors: Antje
authors: A
authors: Ulrich
authors: Martina
authors: M
authors: Vockerodt
authors: Frederike
authors: F
authors: von Bonin
authors: Wolfram
authors: W
authors: Klapper
authors: Katharina
authors: K
authors: Meyer
authors: Sonja
authors: S
authors: Eberth
authors: Tobias
authors: T
authors: Pukrop
authors: Rainer
authors: R
authors: Spang
authors: Lorenz
authors: L
authors: Trümper
authors: Dieter
authors: D
authors: Kube
mesh_terms: D002051:Burkitt Lymphoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D014066:Palatine Tonsil / Q000378:metabolism / Q000473:pathology
chemical_list: C497573:LEF1 protein, human; D051821:Lymphoid Enhancer-Binding Factor 1