title: Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin.
abstract: BACKGROUND
  For regenerative therapy using induced pluripotent stem cell (iPSC) technology, cell type of origin to be reprogrammed should be chosen based on accessibility and reprogramming efficiency. Some studies report that iPSCs exhibited a preference for differentiation into their original cell lineages, while others did not. Therefore, the type of cell which is most appropriate as a source for iPSCs needs to be clarified.
  
  
  RESULTS
  Genetically matched human iPSCs from different origins were generated using bone marrow stromal cells (BMSCs) and dermal fibroblasts (DFs) of the same donor, and global gene expression profile, DNA methylation status, and differentiation properties into the chondrogenic and osteogenic lineage of each clone were analyzed. Although genome-wide profiling of DNA methylation suggested tissue memory in iPSCs, genes expressed differentially in BMSCs and DFs were equally silenced in our bona fide iPSCs. After cell-autonomous and induced differentiation, each iPSC clone exhibited various differentiation properties, which did not correlate with cell-of-origin.
  
  
  CONCLUSIONS
  The reprogramming process may remove the difference between DFs and BMSCs at least for chondrogenic and osteogenic differentiation. Qualified and genetically matched human iPSC clone sets established in this study are valuable resources for further basic study of clonal differences.
authors: Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
authors: Akira
authors: A
authors: Nasu
authors: Makoto
authors: M
authors: Ikeya
authors: Takuya
authors: T
authors: Yamamoto
authors: Akira
authors: A
authors: Watanabe
authors: Yonghui
authors: Y
authors: Jin
authors: Yoshihisa
authors: Y
authors: Matsumoto
authors: Kazuo
authors: K
authors: Hayakawa
authors: Naoki
authors: N
authors: Amano
authors: Shingo
authors: S
authors: Sato
authors: Kenji
authors: K
authors: Osafune
authors: Tomoki
authors: T
authors: Aoyama
authors: Takashi
authors: T
authors: Nakamura
authors: Tomohisa
authors: T
authors: Kato
authors: Junya
authors: J
authors: Toguchida
mesh_terms: D001846:Bone Development / Q000235:genetics*; D002356:Cartilage / Q000166:cytology / Q000254:growth & development; D002454:Cell Differentiation; D019070:Cell Lineage; D060965:Clonal Evolution / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology
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