title: DNA mismatch repair protein MSH2 dictates cellular survival in response to low dose radiation in endometrial carcinoma cells.
abstract: DNA repair and G2-phase cell cycle checkpoint responses are involved in the manifestation of hyper-radiosensitivity (HRS). The low-dose radioresponse of MSH2 isogenic endometrial carcinoma cell lines was examined. Defects in cell cycle checkpoint activation and the DNA damage response in irradiated cells (0.2 Gy) were evaluated. HRS was expressed solely in MSH2+ cells and was associated with efficient activation of the early G2-phase cell cycle checkpoint. Maintenance of the arrest was associated with persistent MRE11, γH2AX, RAD51 foci at 2 h after irradiation. Persistent MRE11 and RAD51 foci were also evident 24 h after 0.2 Gy. MSH2 significantly enhances cell radiosensitivity to low dose IR.
authors: Radiation and Urologic Oncology, Prostate Molecular Oncology Research Group, Academic Unit of Clinical and Molecular Oncology, Institute of Molecular Medicine, Trinity College Dublin, Dublin 8, Ireland.
authors: Lynn M
authors: LM
authors: Martin
authors: Brian
authors: B
authors: Marples
authors: Anthony M
authors: AM
authors: Davies
authors: Ann
authors: A
authors: Atzberger
authors: Connla
authors: C
authors: Edwards
authors: Thomas H
authors: TH
authors: Lynch
authors: Donal
authors: D
authors: Hollywood
authors: Laure
authors: L
authors: Marignol
mesh_terms: D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects*; D053843:DNA Mismatch Repair; D004268:DNA-Binding Proteins / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints; D015870:Gene Expression; D006657:Histones / Q000378:metabolism; D006801:Humans; D000076228:MRE11 Homologue Protein; D051718:MutS Homolog 2 Protein / Q000235:genetics / Q000378:metabolism*; D011836:Radiation Tolerance; D059767:Recombinational DNA Repair
chemical_list: D004268:DNA-Binding Proteins; C468783:H2AX protein, human; D006657:Histones; C121766:MRE11 protein, human; D000076228:MRE11 Homologue Protein; C497172:MSH2 protein, human; D051718:MutS Homolog 2 Protein