title: Surface plasmon resonance sensor for phosmet of agricultural products at the ppt detection level.
abstract: A surface plasmon resonance (SPR) immunoassay using a PH-BSA immobilized sensor chip was developed to measure phosmet in food samples. The in-competitive inhibition assay showed highly sensitive and good specificity of the cross-reactivity with analogue structure pesticides. The biosensor exhibited a linear detection range from 8.0 to 60.0 ng/L of phosmet with a lower detection limit of 1.6 ng/L (S/N = 3). The sensitivity obtained with the present SPR affinity biosensor was significantly higher than most of the sensors reported with different measurement methodologies for phosmet. A recovery test of pesticide quantification in peaches, apples, cabbages, and rapes was also studied. Good recoveries (86.4-102.8%) and coefficients of variation (CVs) (5.1-12.6%) were obtained in all cases. The SPR biosensor assay method was compared with cd-ELISA in terms of analysis time, antibody dosage, recoveries, precision, detection limit, pretreatment, and testing costs, and clear advantages could be seen over the traditional ELISA-based detection systems. The developed SPR method was suitable for the rapid quantitative or qualitative determination of phosmet in agricultural products.
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University , 300387, PR China.
authors: Yang
authors: Y
authors: Song
authors: Ming
authors: M
authors: Liu
authors: Shuo
authors: S
authors: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods; D010575:Pesticides / Q000032:analysis*; D010706:Phosmet / Q000032:analysis*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010575:Pesticides; D010706:Phosmet