title: The venom of the fishing spider Dolomedes sulfurous contains various neurotoxins acting on voltage-activated ion channels in rat dorsal root ganglion neurons.
abstract: Dolomedes sulfurous is a venomous spider distributed in the south of China and characterized with feeding on fish. The venom exhibits great diversity and contains hundreds of peptides as revealed by off-line RP-HPLC/MALDI-TOF-MS analysis. The venom peptides followed a triple-modal distribution, with 40.7% of peptides falling in the mass range of 1000-3000 Da, 25.6% peptides in the 7000-9000 Da range and 23.5% peptides in the 3000-5000 Da range. This distribution modal is rather different from these of peptides from other spider venoms analyzed. The venom could inhibit voltage-activated Na(+), K(+) and Ca(2+) channels in rat DRG neurons as revealed by voltage-clamp analysis. Significantly, the venom exhibited inhibitory effects on TTX-R Na(+) and T-type Ca(2+) currents, suggesting that there exist both channel antagonists which might be valuable tools for investigation of both channels and drug development. Additionally, intrathoracically injection of venom could cause serve neurotoxic effects on zebrafish and death at higher concentrations. The LD50 value was calculated to be 28.8 μg/g body weight. Our results indicated that the venom of D. sulfurous contain diverse neurotoxins which serve to capture prey. Intensive studies will be necessary to investigate the structures and functions of specific peptides of the venom in the future.
authors: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.
authors: Hengyun
authors: H
authors: Wang
authors: Fan
authors: F
authors: Zhang
authors: Dan
authors: D
authors: Li
authors: Shiyan
authors: S
authors: Xu
authors: Juan
authors: J
authors: He
authors: Hai
authors: H
authors: Yu
authors: Jiayan
authors: J
authors: Li
authors: Zhonghua
authors: Z
authors: Liu
authors: Songping
authors: S
authors: Liang
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000737:chemistry / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005727:Ganglia, Spinal / Q000187:drug effects*; D007928:Lethal Dose 50; D009498:Neurotoxins / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D024642:Potassium Channels, Voltage-Gated / Q000737:chemistry / Q000187:drug effects; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013111:Spider Venoms / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D061566:Voltage-Gated Sodium Channels / Q000737:chemistry / Q000187:drug effects; D015027:Zebrafish / Q000502:physiology
chemical_list: D015220:Calcium Channels; D009498:Neurotoxins; D024642:Potassium Channels, Voltage-Gated; D013111:Spider Venoms; D061566:Voltage-Gated Sodium Channels