title: Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
abstract: Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and explored due to the lacking of genetic information. Here, we present the genomic and secretomic analyses of Penicillium decumbens that has been used in industrial production of lignocellulolytic enzymes in China for more than fifteen years. Comparative genomics analysis with the phylogenetically most similar species Penicillium chrysogenum revealed that P. decumbens has evolved with more genes involved in plant cell wall degradation, but fewer genes in cellular metabolism and regulation. Compared with the widely used cellulase producer Trichoderma reesei, P. decumbens has a lignocellulolytic enzyme system with more diverse components, particularly for cellulose binding domain-containing proteins and hemicellulases. Further, proteomic analysis of secretomes revealed that P. decumbens produced significantly more lignocellulolytic enzymes in the medium with cellulose-wheat bran as the carbon source than with glucose. The results expand our knowledge on the genetic information of lignocellulolytic enzyme systems in Penicillium species, and will facilitate rational strain improvement for the production of highly efficient enzyme systems used in lignocellulose utilization from Penicillium species.
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China.
authors: Guodong
authors: G
authors: Liu
authors: Lei
authors: L
authors: Zhang
authors: Xiaomin
authors: X
authors: Wei
authors: Gen
authors: G
authors: Zou
authors: Yuqi
authors: Y
authors: Qin
authors: Liang
authors: L
authors: Ma
authors: Jie
authors: J
authors: Li
authors: Huajun
authors: H
authors: Zheng
authors: Shengyue
authors: S
authors: Wang
authors: Chengshu
authors: C
authors: Wang
authors: Luying
authors: L
authors: Xun
authors: Guo-Ping
authors: GP
authors: Zhao
authors: Zhihua
authors: Z
authors: Zhou
authors: Yinbo
authors: Y
authors: Qu
mesh_terms: D056804:Biofuels; D019149:Bioreactors; D001709:Biotechnology; D044602:Cellulases / Q000096:biosynthesis* / Q000235:genetics; D002681:China; D023281:Genomics / Q000379:methods; D008031:Lignin / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D056804:Biofuels; D014157:Transcription Factors; C036909:lignocellulose; D008031:Lignin; D044602:Cellulases
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