title: Viologen-Phosphorus Dendrimers Inhibit α-Synuclein Fibrillation.
abstract: Inhibition of α-synuclein (ASN) fibril formation is a potential therapeutic strategy in Parkinson's disease and other synucleinopathies. The aim of this study was to examine the role of viologen-phosphorus dendrimers in the α-synuclein fibrillation process and to assess the structural changes in α-synuclein under the influence of dendrimers. ASN interactions with phosphonate and pegylated surface-reactive viologen-phosphorus dendrimers were examined by measuring the zeta potential, which allowed determining the number of dendrimer molecules that bind to the ASN molecule. The fibrillation kinetics and the structural changes were examined using ThT fluorescence and CD spectroscopy. Depending on the concentration of the used dendrimer and the nature of the reactive groups located on the surface, ASN fibrillation kinetics can be significantly reduced, and even, in the specific case of phosphonate dendrimers, the fibrillation can be totally inhibited at low concentrations. The presented results indicate that viologen-phosphorus dendrimers are able to inhibit ASN fibril formation and may be used as fibrillar regulating agents in neurodegenerative disorders.
authors: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland. milowska@biol.uni.lodz.pl
authors: Katarzyna
authors: K
authors: Milowska
authors: Justyna
authors: J
authors: Grochowina
authors: Nadia
authors: N
authors: Katir
authors: Abdelkrim
authors: A
authors: El Kadib
authors: Jean-Pierre
authors: JP
authors: Majoral
authors: Maria
authors: M
authors: Bryszewska
authors: Teresa
authors: T
authors: Gabryelak
mesh_terms: D002942:Circular Dichroism; D050091:Dendrimers / Q000737:chemistry*; D006801:Humans; D010758:Phosphorus / Q000737:chemistry*; D014755:Viologens / Q000737:chemistry*; D051844:alpha-Synuclein / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D014755:Viologens; D051844:alpha-Synuclein; D010758:Phosphorus