title: Hsp27: a novel therapeutic target for pediatric M4/M5 acute myeloid leukemia.
abstract: Heat shock protein 27 (Hsp27), a member of the heat shock protein (Hsp)             family, is critical in the regulation of cancer development, progression and chemotherapy             resistance. However, the role of Hsp27 in the pathogenesis of pediatric acute             leukemia (AL) remains unknown. In this study, we evaluated the expression levels             of Hsp27 in bone marrow samples from 94 children with newly diagnosed acute lymphoblastic             leukemia (ALL), acute myeloid leukemia (AML) and 5 leukemia cell lines. Additionally,             we transfected a target-specific siRNA duplex against Hsp27 into leukemia cells,             and examined the chemosensitivity and cell apoptosis in the response to antitumor             drugs. Hsp27 was abundantly expressed in newly diagnosed AML-M4/M5 bone marrow             mononuclear cells (BMMCs) and THP-1, OCI/AML-3 leukemia cell lines. Furthermore,             its expression was positively correlated with the clinical status in pediatric             M4/M5 subtypes. Knockdown of Hsp27 expression increased the chemosensitivity of             leukemia cells and the anticancer drug-induced apoptosis. These results support             the theory that Hsp27 plays a contributory role in the pathogenesis of pediatric             AML-M4/M5. Therefore, Hsp27 may be exploited as a new target for enhancing the             efficacy of chemotherapeutic drugs against leukemia.
authors: Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors: Liangchun
authors: L
authors: Yang
authors: Lizhi
authors: L
authors: Cao
authors: Minghua
authors: M
authors: Yang
authors: Daolin
authors: D
authors: Tang
authors: Rui
authors: R
authors: Kang
authors: Xie
authors: X
authors: Min
authors: Shan
authors: S
authors: Zhu
authors: Yan
authors: Y
authors: Yu
mesh_terms: D000293:Adolescent; D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000235:genetics; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002648:Child; D002675:Child, Preschool; D005260:Female; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D007223:Infant; D007948:Leukemia, Monocytic, Acute / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D015479:Leukemia, Myelomonocytic, Acute / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D008297:Male; D018832:Molecular Chaperones; D058990:Molecular Targeted Therapy; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D034741:RNA, Small Interfering