title: Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites.
abstract: Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using γ-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MTT assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37°C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 μmol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants.
authors: Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors: Zeljka
authors: Z
authors: Jovanović
authors: Aleksandra
authors: A
authors: Radosavljević
authors: Zorica
authors: Z
authors: Kačarević-Popović
authors: Jasmina
authors: J
authors: Stojkovska
authors: Aleksandra
authors: A
authors: Perić-Grujić
authors: Mirjana
authors: M
authors: Ristić
authors: Ivana Z
authors: IZ
authors: Matić
authors: Zorica D
authors: ZD
authors: Juranić
authors: Bojana
authors: B
authors: Obradovic
authors: Vesna
authors: V
authors: Mišković-Stanković
mesh_terms: D040761:Biomimetic Materials / Q000378:metabolism; D019149:Bioreactors; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011759:Pyrrolidines / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011145:Polyvinyls; D011759:Pyrrolidines; C505150:poly(N-vinylpyrrolidine); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012834:Silver