title: Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes.
abstract: We present the efficient and stable encapsulation of doxorubicin within pH sensitive polymeric vesicles (polymersomes) for intracellular and nuclear delivery to melanoma cells. We demonstrate that PMPC25-PDPA70 polymersomes can encapsulate doxorubicin for long periods of time without significant drug release. We demonstrate that empty polymersomes are non-toxic and that they are quickly and more efficiently internalised by melanoma cells compared to healthy cells. Encapsulated doxorubicin has a strong cytotoxic effect on both healthy and cancerous cells, but when encapsulated it had a preferential effect on melanoma cells indicating that this formulation can be used to achieve an enhanced drug delivery to cancerous cells rather than to the healthy surrounding cells.
authors: The Krebs Institute, University of Sheffield, Sheffield, UK.
authors: Carla
authors: C
authors: Pegoraro
authors: Denis
authors: D
authors: Cecchin
authors: Lorena Simon
authors: LS
authors: Gracia
authors: Nicholas
authors: N
authors: Warren
authors: Jeppe
authors: J
authors: Madsen
authors: Steven P
authors: SP
authors: Armes
authors: Andrew
authors: A
authors: Lewis
authors: Sheila
authors: S
authors: Macneil
authors: Giuseppe
authors: G
authors: Battaglia
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008689:Methacrylates / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D010767:Phosphorylcholine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D000903:Antibiotics, Antineoplastic; D008689:Methacrylates; D011108:Polymers; D011109:Polymethacrylic Acids; C511861:poly(2-(diisopropylamino)ethyl methacrylate); C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; D004317:Doxorubicin