title: Experimental vaginal infection of goats with semen contaminated with the "CPG" strain of Toxoplasma gondii.
abstract: The objective was to characterize the transmission of Toxoplasma gondii in goats experimentally infected vaginally with semen contaminated with the CPG strain (genotype III). Ten female goats were randomly allocated into 2 groups (G1 and G2), each with 5 animals, and inseminated during estrus. Goats in G1 were inseminated with semen containing 1 × 10(5) tachyzoites, whereas those in G2 (control) were inseminated with semen free from tachyzoites (insemination = day 0). In G1, seroconversion (indirect immunofluorescence reaction) and DNA (polymerase chain reaction) in the blood was present in 4/5 and 3/5, respectively, from the 7th day. In G2, all goats were negative in all tests. Embryonic reabsorption occurred in 4 of 5 goats from G1 between days 21 and 49. In conclusion, artificial vaginal insemination with semen containing tachyzoites of T. gondii -infected goats and is a potential transmission route of this parasite through semen.
authors: Programa de Pós, graduação em Biociência Animal, Universidade Federal Rural de Pernambuco, Dois Irmãos, Recife, PE, Brazil.
authors: Flaviana Santos
authors: FS
authors: Wanderley
authors: Wagnner José Nascimento
authors: WJ
authors: Porto
authors: Diogo Ribeiro
authors: DR
authors: Câmara
authors: Nadine Louise Nicolau
authors: NL
authors: da Cruz
authors: Bruna Catarina de Oliveira
authors: BC
authors: Feitosa
authors: Roberta Lemos
authors: RL
authors: Freire
authors: Erica Paes Barreto Xavier
authors: EP
authors: de Moraes
authors: Rinaldo Aparecido
authors: RA
authors: Mota
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D016054:DNA, Protozoan / Q000097:blood; D005260:Female; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission*; D006041:Goats; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D051379:Mice; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary; D011258:Pregnancy Tests / Q000662:veterinary; D012661:Semen / Q000469:parasitology*; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000453:epidemiology / Q000635:transmission*; D016216:Ultrasonography, Prenatal / Q000662:veterinary
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan