title: Circulating tumor cell microseparator based on lateral magnetophoresis and immunomagnetic nanobeads.
abstract: This paper presents a circulating tumor cell (CTC) microseparator for isolation of CTCs from human peripheral blood using immunomagnetic nanobeads with bound antiepithelial cell adhesive molecule (EpCAM) antibodies that specifically bind to epithelial cancer cells. The isolation is performed through lateral magnetophoresis, which is induced by high-gradient magnetic separation technology, involving a ferromagnetic wire array inlaid in the bottom substrate of a microchannel. Experimental results showed that the CTC microseparator isolates about 90% of spiked CTCs in human peripheral blood at a flow rate of up to 5 mL/h and purifies to approximately 97%. The overall isolation procedure was completed within 15 min for 200 μL of peripheral blood. CTCs from peripheral blood of patients with breast and lung cancers were isolated with the CTC microseparator, and the results were compared with those of healthy donors. Using a fluorescence-based viability assay, the viability of CTCs isolated from peripheral blood of patients with cancer was observed. In addition, the usefulness of the CTC microseparator for subsequent genetic assay was confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR) amplification of cancer-specific genes using CTCs isolated from patients with cancer.
authors: Department of Nano Engineering, Center for Nano Manufacturing, Inje University, Gimhae 621-749, Republic of Korea.
authors: Seonyoung
authors: S
authors: Kim
authors: Song-I
authors: SI
authors: Han
authors: Min-Jae
authors: MJ
authors: Park
authors: Chang-Wan
authors: CW
authors: Jeon
authors: Young-Don
authors: YD
authors: Joo
authors: In-Hak
authors: IH
authors: Choi
authors: Ki-Ho
authors: KH
authors: Han
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology; D002465:Cell Movement; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008175:Lung Neoplasms / Q000473:pathology; D060526:Magnetic Fields; D036103:Nanotechnology / Q000379:methods*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*