title: Elemental composition of dog foods using nitric acid and simulated gastric digestions.
abstract: Eighteen dry dog foods obtained commercially in the United States were digested using microwave assisted nitric acid digestion and a simulated gastric digestion. Digests were analysed for 23 elements using inductively coupled plasma-mass spectrometry. Data, expressed as dry matter concentrations, were compared to published nitric acid digestion results. Nitric acid data obtained in the present study were not statistically different from published data, with the exception of Mo, Sn, Sb, Tl and Th. However, significant differences in individual intra-sample results were observed between published studies and the present work. Simulated gastric digestions demonstrated lower extraction efficiencies (<50% nitric acid digestions) that were statistically significant. Much lower bioavailability was observed for Al, Ba and Pb. In general, elemental concentrations were determined to be lower than the appropriate Mineral Tolerance Limit or consistent with background concentrations in foodstuffs. Evaluation against Reference Doses (RfDs) showed concentrations for many elements obtained by nitric acid digestion to be above RfD levels. However, the respective simulated gastric digestion data were below or only moderately elevated above RfDs. Only arsenic displayed median and maximum concentrations at factors of five and ten above the relevant RfD.
authors: Department of Chemistry and Chemical Engineering, Royal Military College of Canada, P.O. Box 17000 Stn. Forces, Kingston, ON, Canada. david.kelly@rmc.ca
authors: David G
authors: DG
authors: Kelly
authors: Steven D
authors: SD
authors: White
authors: Ron D
authors: RD
authors: Weir
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001682:Biological Availability; D004285:Dogs; D005753:Gastric Mucosa / Q000378:metabolism*; D013058:Mass Spectrometry; D008670:Metals / Q000032:analysis* / Q000493:pharmacokinetics; D017942:Nitric Acid / Q000737:chemistry*; D013818:Thermogravimetry
chemical_list: D008670:Metals; D017942:Nitric Acid