title: 2',4'-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides.
abstract: DNA-based aptamers that contain 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) over the entire length were successfully obtained using a capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX) method. A modified DNA library was prepared with an enzyme mix of KOD Dash and KOD mutant DNA polymerases. Forty 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) aptamers were isolated from an enriched pool and classified into six groups according to their sequence. 2',4'-BNA/LNA aptamers of groups V and VI bound human thrombin with K(d) values in the range of several 10 nanomolar levels.
authors: Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors: Yuuya
authors: Y
authors: Kasahara
authors: Yuuta
authors: Y
authors: Irisawa
authors: Hiroaki
authors: H
authors: Ozaki
authors: Satoshi
authors: S
authors: Obika
authors: Masayasu
authors: M
authors: Kuwahara
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001952:Bridged-Ring Compounds / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D006801:Humans; D009711:Nucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012265:Ribonucleotides / Q000737:chemistry* / Q000235:genetics; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: C527205:2',4'-BNA(NC); D052157:Aptamers, Nucleotide; D001952:Bridged-Ring Compounds; D009711:Nucleotides; D009841:Oligonucleotides; D012265:Ribonucleotides; C477371:locked nucleic acid; D004259:DNA-Directed DNA Polymerase