title: Whole genome gene expression analysis reveals casiopeína-induced apoptosis pathways.
abstract: Copper-based chemotherapeutic compounds Casiopeínas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis-in a process mediated by reactive oxygen species-for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions before establishing settings and parameters needed for their wide clinical application. In order to shorten the gap in this respect, we present a model proposal centered in the role played by intrinsic (or mitochondrial) apoptosis triggered by oxidative stress caused by the chemotherapeutic agent. This model has been inferred based on genome wide expression profiling in cervix cancer (HeLa) cells, as well as statistical and computational tests, validated via functional experiments (both in the same HeLa cells and also in a Neuroblastoma model, the CHP-212 cell line) and assessed by means of data mining studies.
authors: Computational Genomics Department, National Institute of Genomic Medicine, México City, México.
authors: Alejandra Idan
authors: AI
authors: Valencia-Cruz
authors: Laura I
authors: LI
authors: Uribe-Figueroa
authors: Rodrigo
authors: R
authors: Galindo-Murillo
authors: Karol
authors: K
authors: Baca-López
authors: Anllely G
authors: AG
authors: Gutiérrez
authors: Adriana
authors: A
authors: Vázquez-Aguirre
authors: Lena
authors: L
authors: Ruiz-Azuara
authors: Enrique
authors: E
authors: Hernández-Lemus
authors: Carmen
authors: C
authors: Mejía
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015894:Genome, Human; D005978:Glutathione / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D058977:Molecular Sequence Annotation; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; C505569:casiopeina II-glycine; D020169:Caspases; D005978:Glutathione
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