title: Expression of innate immune genes, proteins and microRNAs in lung tissue of pigs infected experimentally with influenza virus (H1N2).
abstract: This study aimed at providing a better understanding of the involvement of innate immune factors, including miRNA, in the local host response to influenza virus infection. Twenty pigs were challenged by influenza A virus subtype H1N2. Expression of microRNA (miRNA), mRNA and proteins were quantified in lung tissue at different time points after challenge (24 h, 72 h and 14 d post-infection (p.i.). Several groups of genes were significantly regulated according to time point and infection status including pattern recognition receptors (TLR2, TLR3, TLR7, retinoic acid-inducible gene I, melanoma differentiation associated protein-5), IFN and IFN-induced genes (IFN-β, IFN-γ, IRF7, STAT1, ISG15 and OASL), cytokines (IL-1 β, IL-1RN, IL-6, IL-7, IL-10, IL-12A, TNF-α, CCL2, CCL3 and CXCL10) and several acute phase proteins. Likewise, the following miRNAs were differentially expressed in one or more time groups compared with the control pigs: miR-15a, miR-21, miR-146, miR-206, miR-223 and miR-451. At d 1 p.i. lung tissue protein levels of IL-6, IL-12 and IFN-α were significantly increased compared with the control group, and haptoglobin and C-reactive protein were significantly increased at d 3 p.i. Our results suggest that, in addition to a wide range of innate immune factors, miRNAs may also be involved in controlling acute influenza infection in pigs.
authors: 1Innate Immunology Group, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark.
authors: Kerstin
authors: K
authors: Skovgaard
authors: Susanna
authors: S
authors: Cirera
authors: Ditte
authors: D
authors: Vasby
authors: Agnieszka
authors: A
authors: Podolska
authors: Solvej Ø
authors: SØ
authors: Breum
authors: Ralf
authors: R
authors: Dürrwald
authors: Michael
authors: M
authors: Schlegel
authors: Peter M H
authors: PM
authors: Heegaard
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D007113:Immunity, Innate / Q000235:genetics; D057221:Influenza A Virus, H1N2 Subtype / Q000276:immunology*; D007372:Interferons / Q000235:genetics / Q000378:metabolism; D008168:Lung / Q000276:immunology* / Q000821:virology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D023421:Models, Animal; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology*; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D000209:Acute-Phase Proteins; D016207:Cytokines; D035683:MicroRNAs; D051192:Receptors, Pattern Recognition; D007372:Interferons
keywords: H1N2; influenza; innate; miRNA; pig