title: Cyclic parenteral nutrition does not change the intestinal microbiota in patients with short bowel syndrome.
abstract: OBJECTIVE
  To characterize of the intestinal microbiota of patients with short bowel syndrome (SBS) admitted to the Metabolic Unit of a University Hospital.
  
  
  METHODS
  Fecal samples were evaluated, and biochemical tests were conducted only in the case of SBS patients. The nutritional status was assessed via anthropometric measurements and evaluation of food intake by means of a food questionnaire. The pathogenic strains were detected with the aid of cultures and specific biochemical tests in aerobic medium, for determination of species belonging to the Family enterobacteriaceae. Anti-sera were applied to each isolated E. coli strain, for determination of their possible pathogenicity. Molecular methodology was employed for establishment of the intestinal bacterial microbiota profile
  
  
  RESULTS
  A lower amount of microorganisms of the family enterobacteriaceae per gram of stool was observed in the case of patients with SBS. However, molecular analysis showed maintenance of the bacterial species ratio, which is equivalent to a healthy intestinal microbiota.
  
  
  CONCLUSIONS
  Despite the massive removal of the small bowel, frequent use of antibiotics, immune system depression, presence of non-digested food in the gastrointestinal tract, and accelerated intestinal transit, the ratio between intestinal bacterial species remain similar to normality.
authors: Clinical Nutrition Division, Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Eduarda de Castro
authors: Ede C
authors: Furtado
authors: Julio Sergio
authors: JS
authors: Marchini
authors: Carol Kobori da
authors: CK
authors: Fonseca
authors: Paulo Sérgio Rodrigues
authors: PS
authors: Coelho
authors: Mayra Gonçalves
authors: MG
authors: Menegueti
authors: Maria
authors: M
authors: Auxiliadora-Martins
authors: Anibal
authors: A
authors: Basile-Filho
authors: Vivian Marques Miguel
authors: VM
authors: Suen
mesh_terms: D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004269:DNA, Bacterial; D002149:Energy Intake; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000382:microbiology* / Q000601:surgery; D064307:Microbiota; D009752:Nutritional Status; D010288:Parenteral Nutrition / Q000379:methods*; D012778:Short Bowel Syndrome / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial