title: Seed train development for the fermentation of bagasse from sweet sorghum and sugarcane using a simplified fermentation process.
abstract: A process was developed for seed culture expansion (3.6 million-fold) using 5% of the hemicellulose hydrolysate from dilute acid pretreatment as the sole organic nutrient and source of sugar. Hydrolysate used for seed growth was neutralized with ammonia and combined with 1.0mM sodium metabisulfite immediately before inoculation. This seed protocol was tested with phosphoric acid pretreated sugarcane and sweet sorghum bagasse using a simplified process with co-fermentation of fiber, pentoses, and hexoses in a single vessel (SScF). A 6h liquefaction (L) step improved mixing prior to inoculation. Fermentations (L+SScF process) were completed in 72 h with high yields (>80 gal/US ton). Ethanol titers for this L+SScF process ranged from 24 g/L to 32 g/L, and were limited by the bagasse concentration (10% dry matter).
authors: Dept Microbiology & Cell Science, Univ Florida, Box 110700, Gainesville, FL 32611, United States.
authors: C C
authors: CC
authors: Geddes
authors: M T
authors: MT
authors: Mullinnix
authors: I U
authors: IU
authors: Nieves
authors: R W
authors: RW
authors: Hoffman
authors: W J
authors: WJ
authors: Sagues
authors: S W
authors: SW
authors: York
authors: K T
authors: KT
authors: Shanmugam
authors: J E
authors: JE
authors: Erickson
authors: W E
authors: WE
authors: Vermerris
authors: L O
authors: LO
authors: Ingram
mesh_terms: D002482:Cellulose / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D031786:Saccharum / Q000382:microbiology*; D012639:Seeds / Q000737:chemistry*; D045868:Sorghum / Q000382:microbiology*; D013227:Steam
chemical_list: D013227:Steam; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse