title: The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting mass-spectrometry-based glycoanalytic data.
abstract: The MIRAGE guidelines are being developed in response to a critical need in the glycobiology community to clarify glycoanalytic results so that they are more readily evaluated (in terms of their scope and depth) and to facilitate the reproduction of important results in the laboratory. The molecular and biological complexity of the glycosylation process makes thorough reporting of the results of a glycomics experiment a highly challenging endeavor. The resulting data specify the identity and quantity of complex structures, the precise molecular features of which are sometimes inferred using prior knowledge, such as familiarity with a particular biosynthetic mechanism. Specifying the exact methods and assumptions that were used to assign and quantify reported structures allows the interested scientist to appreciate the scope and depth of the analysis. Mass spectrometry (MS) is the most widely used tool for glycomics experiments. The interpretation and reproducibility of MS-based glycomics data depend on comprehensive meta-data describing the instrumentation, instrument setup, and data acquisition protocols. The MIRAGE guidelines for MS-based glycomics have been designed to facilitate the collection and sharing of this critical information in order to assist the glycoanalyst in generating data sets with maximum information content and biological relevance.
authors: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
authors: Daniel
authors: D
authors: Kolarich
authors: Erdmann
authors: E
authors: Rapp
authors: Weston B
authors: WB
authors: Struwe
authors: Stuart M
authors: SM
authors: Haslam
authors: Joseph
authors: J
authors: Zaia
authors: Ryan
authors: R
authors: McBride
authors: Sanjay
authors: S
authors: Agravat
authors: Matthew P
authors: MP
authors: Campbell
authors: Masaki
authors: M
authors: Kato
authors: Rene
authors: R
authors: Ranzinger
authors: Carsten
authors: C
authors: Kettner
authors: William S
authors: WS
authors: York
mesh_terms: D000818:Animals; D054794:Glycomics / Q000592:standards*; D006023:Glycoproteins / Q000737:chemistry; D017408:Guidelines as Topic; D006801:Humans; D013058:Mass Spectrometry / Q000592:standards*; D058996:Quality Improvement; D012015:Reference Standards
chemical_list: D006023:Glycoproteins
references: Nat Chem Biol. 2012 Jul;8(7):661-8
references: 22683610
references: Nat Biotechnol. 2007 Aug;25(8):887-93
references: 17687369
references: Trends Biochem Sci. 2005 Jan;30(1):11-2
references: 15653320
references: Anal Biochem. 2000 Oct 1;285(1):64-75
references: 10998264
references: Nat Genet. 2001 Dec;29(4):365-71
references: 11726920
references: Nat Biotechnol. 2008 Aug;26(8):889-96
references: 18688244