title: FoxQ1 promotes glioma cells proliferation and migration by regulating NRXN3 expression.
abstract: BACKGROUND
  Forkhead box Q1 (FoxQ1) is a member of the forkhead transcription factor family, and it has recently been found to participate in cancer development. However, whether FoxQ1 expression contributes to glioma development and progression is not known. We investigate FoxQ1 expression in gliomas and the role of FoxQ1 during tumorgenesis.
  
  
  METHODS
  Reverse transcription quantitative real-time PCR (RT-qPCR) and Western blot were used to determine the FoxQ1 and Neurexins 3 (NRXN3) expression in gliomas. Chromatin immunoprecipitation (ChIP) and luciferase assays were used to determine the regulation between FoxQ1 and NRXN3. We established depleted FoxQ1 stable clones in U-87MG cells and overexpressed FoxQ1 stable clones in SW1088 cells. MTT and transwell were used to evaluate the ability of proliferation and migration, respectively.
  
  
  RESULTS
  FoxQ1 mRNA and protein were up-regulated in gliomas and negatively related to the NRXN3 expression (r = -0.373, P = 0.042). FoxQ1 directly binds to NRXN3 promoter region and suppresses the promoter activity. Furthermore, the ability of proliferation and migration is reduced in depleted FoxQ1 cells.
  
  
  CONCLUSIONS
  FoxQ1 promotes glioma cell proliferation and migration by down-regulation of NRXN3 expression.
authors: Institute of Traumatic Brain Injury and Neurology of the Chinese People's Armed Police Forces, Tianjin, China.
authors: Hong-Tao
authors: HT
authors: Sun
authors: Shi-Xiang
authors: SX
authors: Cheng
authors: Yue
authors: Y
authors: Tu
authors: Xiao-Hong
authors: XH
authors: Li
authors: Sai
authors: S
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014183:Transplantation, Heterologous
chemical_list: C484579:FOXQ1 protein, human; D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins; C082354:neurexin IIIalpha
references: Neuron. 1995 Mar;14(3):497-507
references: 7695896
references: Cancer Res. 2005 Jun 15;65(12):5181-9
references: 15958562
references: Nucleic Acids Res. 1997 May 1;25(9):1715-9
references: 9108152
references: Mol Biol Rep. 2012 Mar;39(3):2915-9
references: 21688152
references: Cancer Res. 2011 Apr 15;71(8):3076-86
references: 21346143
references: Mol Cancer Res. 2010 Nov;8(11):1558-67
references: 20881009
references: Dev Biol. 2002 Oct 1;250(1):1-23
references: 12297093
references: CA Cancer J Clin. 2010 Sep-Oct;60(5):277-300
references: 20610543
references: Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6410-4
references: 8341647
references: Int J Oncol. 2004 Nov;25(5):1495-500
references: 15492844
references: Science. 1992 Jul 3;257(5066):50-6
references: 1621094
references: Genomics. 2002 Apr;79(4):587-97
references: 11944992
references: Cancer Res. 2006 Feb 1;66(3):1712-20
references: 16452231
references: Cell Mol Life Sci. 2005 Feb;62(4):397-409
references: 15719167
references: PLoS One. 2012;7(6):e39937
references: 22761930
references: Cell. 2007 Jan 26;128(2):309-23
references: 17254969
references: Cancer Res. 2011 Feb 15;71(4):1292-301
references: 21285253
references: Nat Cell Biol. 2005 Feb;7(2):126-36
references: 15654331
references: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D91-7
references: 15608292
references: Biochem Biophys Res Commun. 2002 Oct 18;298(1):151-5
references: 12379233
references: J Biol Chem. 2000 Oct 6;275(40):31162-70
references: 10896677
references: J Biol Chem. 2006 Sep 29;281(39):29245-55
references: 16835220
references: Mol Cell Biol. 1997 Mar;17(3):1626-41
references: 9032290
references: Genomics. 2002 Jun;79(6):849-59
references: 12036300
references: Cancer Biol Ther. 2004 Nov;3(11):1081-9; discussion 1090-1
references: 15467436
references: Genesis. 2001 Apr;29(4):163-71
references: 11309849
references: DNA Cell Biol. 2001 Sep;20(9):555-61
references: 11747606
references: J Biol Chem. 2005 Apr 22;280(16):16484-98
references: 15722554
references: Nat Rev Cancer. 2007 Nov;7(11):847-59
references: 17943136
references: Hum Mol Genet. 2007 Dec 1;16(23):2880-91
references: 17804423
references: Cancer Res. 2010 Mar 1;70(5):2053-63
references: 20145154