title: An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation.
abstract: In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5'-UTR, introns, 3'-UTR and terminator elements. Our data revealed that, in contrast to a conventional transgene, an endogene-resembling transgene was more stably expressed and poorly processed into small RNAs. In addition, although both constructs triggered methylation of homologous DNA sequences at similar levels, the endogene-resembling transgene exhibited significantly delayed onset of local and systemic silencing.
authors: RLP AgroScience GmbH, AlPlanta-Institute for Plant Research, 67435 Neustadt, Germany.
authors: Elena
authors: E
authors: Dadami
authors: Mirko
authors: M
authors: Moser
authors: Michele
authors: M
authors: Zwiebel
authors: Gabi
authors: G
authors: Krczal
authors: Michael
authors: M
authors: Wassenegger
authors: Athanasios
authors: A
authors: Dalakouras
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D016960:Agrobacterium tumefaciens / Q000235:genetics; D019175:DNA Methylation; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins; D007438:Introns; D018716:Molecular Mimicry; D010957:Plasmids; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D019076:Transgenes
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins