title: [KDR silencing  supresses  the tumor growth of prostate cancer cell line PC-3 in nude mice].
abstract: OBJECTIVE
  To study the change in the tumor growth of prostate cancer cell line PC-3 in nude mice xenografts after kinase domain receptor (KDR) silencing by RNA interference.
  
  
  METHODS
  A total of 15 5-week-old male nude mice were randomly divided into normal PC-3 cell group (negative control), RNA interference group and pSilencer3.1-NC group, with 5 mice in every group. The nude mice were respectively treated with subcutaneous injection of 0.5 mL (2.0×10(7);/mL) normal PC-3 cells, and the same volume of PC-3 cells transfected with pSilencer3.1-KDR and pSilencer3.1-NC vectors, respectively. By measuring the tumor volumes every 3 d and the tumor weights after 4 weeks, we recorded tumor formation rate, tumor growth rate and mean tumor weight. The expression of KDR at both mRNA and protein levels was detected by RT-PCR and Western blotting, respectively.
  
  
  RESULTS
  Tumor growth was significantly slower in the pSilencer3.1-KDR group than in the negative control group and the pSilencer3.1-NC group. After 4 weeks, the mean volume of tumor in the pSilencer3.1-KDR group was significantly smaller than that in the other two groups (0.28 cm(3); vs 0.715 cm(3); and 0.721 cm(3);, P<0.01), so was the mean weight of tumor (0.14 g vs 0.635 g and 0.648 g, P<0.01). In addition, KDR mRNA and protein expressions significantly decreased.
  
  
  CONCLUSIONS
  The tumor growth in nude mice xenografts can be efficiently inhibited by KDR silencing mediated by RNAi, so the suppression of KDR expression might be a promising strategy for the treatment of human prostate cancer.
authors: Department of Medical Affairs, Chinese PLA 152nd Hospital, Pingdingshan, China.
authors: Guoqi
authors: G
authors: Xie
authors: Weiguo
authors: W
authors: Yi
authors: Xin
authors: X
authors: Liu
authors: Zhaojing
authors: Z
authors: Guo
authors: Yingye
authors: Y
authors: Miao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020868:Gene Silencing; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D034622:RNA Interference; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism
chemical_list: D040301:Vascular Endothelial Growth Factor Receptor-2