title: Antioxidant effect of manganese on the testis structure and sperm parameters of formalin-treated mice.
abstract: Manganese inhibits oxidative stress damage. The aim of this study was to investigate the protective role of manganese on testis structure and sperm parameters in adult mice exposed to formaldehyde (FA). Twenty adult male NMRI mice were selected and randomly divided into four groups: (i) control; (ii) sham; (iii) 'FA'-exposed group; and (iv) 'FA and manganese chloride'-exposed group. The FA-exposed groups received 10 mg kg(-1) FA daily for 14 days, and manganese chloride was just injected intraperitoneally 5 mg kg(-1) on 2nd weeks. Mice were sacrificed, and spermatozoa were collected from the cauda of the right epididymis and analysed for count, motility, morphology and viability. The other testicular tissues were weighed and prepared for histological examination upon removal. Seminiferous tubules, lumen diameters and epithelium thickness were also measured. The findings revealed that FA significantly reduced the testicular weight, sperm count, motility, viability and normal morphology compared with control group (P ≤ 0.05). In addition, seminiferous tubules atrophied and seminiferous epithelial cells disintegrated in the FA group in comparison with the control group (P ≤ 0.05). However, manganese improved the testicular structure and sperm parameters in FA-treated mice testes (P ≤ 0.05). According to the results, manganese may improve and protect mice epididymal sperm parameters and testis structure treated with FA respectively.
authors: Department of Basic Sciences, Payame Noor University, Iran.
authors: S
authors: S
authors: Tajaddini
authors: S
authors: S
authors: Ebrahimi
authors: B
authors: B
authors: Behnam
authors: M
authors: M
authors: Bakhtiyari
authors: M T
authors: MT
authors: Joghataei
authors: M
authors: M
authors: Abbasi
authors: M
authors: M
authors: Amini
authors: S
authors: S
authors: Amanpour
authors: M
authors: M
authors: Koruji
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D005557:Formaldehyde / Q000633:toxicity; D008297:Male; D017895:Manganese Compounds / Q000494:pharmacology*; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000473:pathology
chemical_list: D000975:Antioxidants; D002712:Chlorides; D017895:Manganese Compounds; D005557:Formaldehyde; C025340:manganese chloride
keywords: Formaldehyde; manganese; sperm parameters; testicular structure