title: Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.
abstract: Formation of cilia, microtubule-based structures that function in propulsion and sensation, requires Kif3a, a subunit of Kinesin II essential for intraflagellar transport (IFT). We have found that, Kif3a is also required to organize centrioles. In the absence of Kif3a, the subdistal appendages of centrioles are disorganized and lack p150(Glued) and Ninein. Consequently, microtubule anchoring, centriole cohesion and basal foot formation are abrogated by loss of Kif3a. Kif3a localizes to the mother centriole and interacts with the Dynactin subunit p150(Glued). Depletion of p150(Glued) phenocopies the effects of loss of Kif3a, indicating that Kif3a recruitment of p150(Glued) is critical for subdistal appendage formation. The transport functions of Kif3a are dispensable for subdistal appendage organization as mutant forms of Kif3a lacking motor activity or the motor domain can restore p150(Glued) localization. Comparison to cells lacking Ift88 reveals that the centriolar functions of Kif3a are independent of IFT. Thus, in addition to its ciliogenic roles, Kif3a recruits p150(Glued) to the subdistal appendages of mother centrioles, critical for centrosomes to function as microtubule-organizing centres.
authors: Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, CA 94158-9001, USA.
authors: Andrew
authors: A
authors: Kodani
authors: Maria
authors: M
authors: Salomé Sirerol-Piquer
authors: Allen
authors: A
authors: Seol
authors: Jose Manuel
authors: JM
authors: Garcia-Verdugo
authors: Jeremy F
authors: JF
authors: Reiter
mesh_terms: D000818:Animals; D002502:Centrioles / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D000072159:Dynactin Complex; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; C000606360:DCTN1 protein, human; C000606364:Dctn1 protein, mouse; D000072159:Dynactin Complex; C098623:IFT88 protein, human; C087415:KIF3A protein, human; C490202:Kif3a protein, mouse; D008869:Microtubule-Associated Proteins; C512806:NIN protein, human; C099035:Nin protein, mouse; D009687:Nuclear Proteins; C490898:Tg737Rpw protein, mouse; D025521:Tumor Suppressor Proteins; D016547:Kinesins
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