title: Composition and distribution of elements and ultrastructural topography of a human cardiac calculus.
abstract: Trace elements (TEs) may contribute to the formation of calculi or stones or be involved in the aetiopathogenesis of stone diseases. The compositions and spatial distribution of elements from the inner nucleus to outer crust of the cardiac calculus were investigated by energy-dispersive X-ray fluorescence (EDXRF) spectrometer. The surface topograph, distribution map of elements, elemental and chemical compositions were also determined by environmental scanning electron microscope (ESEM)-energy-dispersive X-ray (EDX) analysis. Twenty-five elements were identifiable from 18 positions on the cardiac calculus by EDXRF spectrometer, in which the highest concentrations of toxic TEs (Ni, Pt, Hg, Sn, Pb, W, Au, Al, Si) and higher levels of essential TEs (Ca, Sr, Cr, P) were detected. A moderate positive Pearson's correlation between TEs concentrations of Mg, Ca or P and location differences from centre to periphery in the cardiac calculus was observed. A positive correlation was also found for Ca/Zn and Ca/Cu, indicating the gradual increase of calcium concentration from inner nucleus to outer crust of cardiac calculus. The drop-like nodules/crystals on the surface of petrous part of cardiac calculus were observed from ESEM analysis. ESEM-EDX analysis determined the calculus to be predominantly composed of calcium hydroxyapatite and cholesterol, as indicated by the petrous surface and drop-like nodules/crystals, respectively. This composition was confirmed using a portable Raman analyser. The spatial distribution analysis indicated a gradual increase in Mg, P and Ca concentrations from the inner nucleus to the outer crust of the cardiac calculus. The major chemical compositions of calcium hydroxyapatite and cholesterol were detected on this cardiac calculus.
authors: Department of Nursing, National Tainan Institute of Nursing, Tainan, Taiwan, Republic of China.
authors: Ching-Li
authors: CL
authors: Cheng
authors: Hsiao-Huang
authors: HH
authors: Chang
authors: Pei-Jung
authors: PJ
authors: Huang
authors: Yu-Ting
authors: YT
authors: Chu
authors: Shan-Yang
authors: SY
authors: Lin
mesh_terms: D000369:Aged, 80 and over; D002137:Calculi / Q000737:chemistry* / Q000648:ultrastructure; D009202:Cardiomyopathies / Q000378:metabolism*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D013052:Spectrometry, X-Ray Emission; D013059:Spectrum Analysis, Raman; D014131:Trace Elements / Q000032:analysis* / Q000378:metabolism*
chemical_list: D014131:Trace Elements