title: Striking stabilization of Rana catesbeiana ribonuclease 3 by guanidine hydrochloride.
abstract: Unfolding by chemical denaturants and the linear extrapolation method are widely used to determine the free energy of proteins. Ribonuclease 3 from bullfrog shows an extraordinary behavior in guanidinium hydrochloride in comparison to its homologues ribonuclease A and onconase with a high transition midpoint of denaturation but an apparently low cooperativity. The analysis of the interdependence of thermal, urea-, and guanidine hydrochloride-induced unfolding revealed that whereas addition of urea resulted in the expected destabilization of all three proteins, guanidine hydrochloride acted diversely: in contrast to ribonuclease A and onconase, both of which were destabilized as expected, low concentrations of guanidine hydrochloride significantly stabilize ribonuclease 3 from bullfrog. This stabilizing effect was endorsed by in silico docking studies.
authors: Martin-Luther University Halle-Wittenberg, Institute of Biochemistry and Biotechnology, Kurt-Mothes Str 3, 06120 Halle, Germany.
authors: Magali
authors: M
authors: Solé
authors: Wolfgang
authors: W
authors: Brandt
authors: Ulrich
authors: U
authors: Arnold
mesh_terms: D029845:Amphibian Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D019791:Guanidine / Q000737:chemistry*; D007700:Kinetics; D062105:Molecular Docking Simulation; D011489:Protein Denaturation; D058767:Protein Unfolding; D011892:Rana catesbeiana / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry
chemical_list: D029845:Amphibian Proteins; D011994:Recombinant Proteins; D014508:Urea; D012260:Ribonucleases; D043244:Ribonuclease III; D012259:Ribonuclease, Pancreatic; D019791:Guanidine; C058703:ranpirnase