title: Highly sensitive and selective detection of silver(I) in aqueous solution with silver(I)-specific DNA and Sybr Green I.
abstract: We report a label-free fluorescence turn-on approach for the sensitive and selective sensing of silver ion Ag(+) based on Ag(+) induced conformational change of cytosine-rich single-stranded DNA. A silver specific oligonucleotide (SSO) undergoes a structural transition from single-stranded DNA (ssDNA) to a C-Ag(+)-C mediated hairpin structure, which can be recognized by a double-stranded DNA (dsDNA) intercalator Sybr Green I (SG). The linear response range for Ag(+) detection was from 1 nM to 100 nM. The method presented here is highly sensitive and a limit of detection of 1 nM was obtained. More importantly, this optical method was successfully used to identify complex sample mixtures.
authors: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
authors: Qing
authors: Q
authors: Yang
authors: Fan
authors: F
authors: Li
authors: Yan
authors: Y
authors: Huang
authors: Hui
authors: H
authors: Xu
authors: Linsheng
authors: L
authors: Tang
authors: Lihua
authors: L
authors: Wang
authors: Chunhai
authors: C
authors: Fan
mesh_terms: D052160:Benzothiazoles; D002414:Cations, Monovalent; D004277:DNA, Single-Stranded / Q000187:drug effects*; D003959:Diamines; D007364:Intercalating Agents / Q000494:pharmacology; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009841:Oligonucleotides / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry*; D011804:Quinolines; D012834:Silver / Q000032:analysis* / Q000494:pharmacology
chemical_list: D052160:Benzothiazoles; D002414:Cations, Monovalent; D004277:DNA, Single-Stranded; D003959:Diamines; D007364:Intercalating Agents; D009841:Oligonucleotides; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D012834:Silver