title: Cytogenetic analysis of Aegilops chromosomes, potentially usable in triticale (X Triticosecale Witt.) breeding.
abstract: Chromosome identification using fluorescence in situ hybridization (FISH) is widely used in cytogenetic research. It is a diagnostic tool helpful in chromosome identification. It can also be used to characterize alien introgressions, when exercised in a combination with genomic in situ hybridization (GISH). This work aims to find chromosome identification of Aegilops species and Aegilops × Secale amphiploids, which can be used in cereal breeding as a source of favourable agronomic traits. Four diploid and two tetraploid Aegilops species and three Aegilops × Secale hybrids were analysed using FISH with pSc119.2, pAs1, 5S rDNA and 25S rDNA clones to differentiate the U-, M-, S(sh)- and D-subgenome chromosomes of Aegilops genus. Additionally, GISH for chromosome categorization was carried out. Differences in the hybridization patterns allowed to identify all U-, M-, S(sh)- and D-subgenome chromosomes. Some differences in localization of the rDNA, pSc119.2 and pAs1 sequences between analogue subgenomes in diploid and tetraploid species and Aegilops × Secale hybrids were detected. The hybridization pattern of the M and S genome was more variable than that of the U and D genome. An importance of the cytogenetic markers in plant breeding and their possible role in chromosome structure, function and evolution is discussed.
authors: Laboratory of Distant Crosses, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznan, Poland. mkwi@igr.poznan.pl
authors: M
authors: M
authors: Kwiatek
authors: H
authors: H
authors: Wiśniewska
authors: B
authors: B
authors: Apolinarska
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods*; D018744:DNA, Plant / Q000737:chemistry / Q000378:metabolism; D002523:Edible Grain / Q000235:genetics*; D005819:Genetic Markers; D018745:Genome, Plant; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000235:genetics
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
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