title: SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair.
abstract: The architecture and mechanical properties of a scaffold for spinal cord injury treatment must provide tissue integration as well as effective axonal regeneration. Previous work has demonstrated the cell-adhesive and growth-promoting properties of the SIKVAV (Ser-Ile-Lys-Val-Ala-Val)-modified highly superporous poly(2-hydroxethyl methacrylate) (PHEMA) hydrogels. The aim of the current study was to optimize the porosity and mechanical properties of this type of hydrogel in order to develop a suitable scaffold for the repair of spinal cord tissue. Three types of highly superporous PHEMA hydrogels with oriented pores of ~60 µm diameter, porosities of 57-68% and equivalent stiffness characterized by elasticity moduli in the range 3-45 kPa were implanted into a spinal cord hemisection, and their integration into the host tissue, as well as the extent of axonal ingrowth into the scaffold pores, were histologically evaluated. The best tissue response was found with a SIKVAV-modified PHEMA hydrogel with 68% porosity and a moderate modulus of elasticity (27 kPa in the direction along the pores and 3.6 kPa in the perpendicular direction). When implanted into a spinal cord transection, the hydrogel promoted tissue bridging as well as aligned axonal ingrowth. In conclusion, a prospective oriented scaffold architecture of SIKVAV-modified PHEMA hydrogels has been developed for spinal cord injury repair; however, to develop an effective treatment for spinal cord injury, multiple therapeutic approaches are needed.
authors: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Šárka
authors: Š
authors: Kubinová
authors: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Daniel
authors: D
authors: Horák
authors: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.|Department of Neurosurgery, Masaryk Hospital, Ústí nad Labem, Czech Republic.
authors: Aleš
authors: A
authors: Hejčl
authors: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Zdeněk
authors: Z
authors: Plichta
authors: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Jiří
authors: J
authors: Kotek
authors: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Vladimír
authors: V
authors: Proks
authors: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Serhiy
authors: S
authors: Forostyak
authors: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.|Department of Neuroscience, 2nd Medical Faculty, Charles University, Prague, Czech Republic.
authors: Eva
authors: E
authors: Syková
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D001672:Biocompatible Materials / Q000737:chemistry; D004548:Elasticity; D049452:Green Fluorescent Proteins / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D009416:Nerve Regeneration; D009842:Oligopeptides / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D016062:Porosity; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000534:rehabilitation* / Q000628:therapy; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D009842:Oligopeptides; C077583:seryl-isoleucyl-lysyl-valyl-alanyl-valinamide; D049452:Green Fluorescent Proteins; D011102:Polyhydroxyethyl Methacrylate
keywords: 2-hydroxyethyl methacrylate; IKVAV (Ile-Lys-Val-Ala-Val) peptide; hydrogel; oriented pores; scaffold; spinal cord repair