title: Bm91 is an envelope component of ODV but is dispensable for the propagation of Bombyx mori nucleopolyhedrovirus.
abstract: Orf91 (Bm91) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene that encodes a predicted 105-amino-acid protein, but its function remains unknown. In the current study, 5'-RACE revealed that the transcription initiation site of Bm91 was - 12 nucleotides upstream of the start codon ATG, transcription of Bm91 was detected from 12 to 96 h postinfection (p.i.) and Bm91 protein was detected from 24 to 96 h p.i. in BmNPV-infected BmN cells. Furthermore, Western blot analysis revealed that Bm91 was in occlusion-derived virus (ODV) but not in budded virus (BV). To investigate the role of Bm91 in baculovirus life cycle, a Bm91-knockout virus was constructed by bacmid recombination in E. coli. Fluorescence and light microscopy showed that the production of BV and occlusion bodies (OBs) in Bm91-deficient-virus-infected BmN cells were similar to those in wild-type-virus-infected ones. Bioassay results showed that genetic deletion of Bm91 did not significantly affect BmNPV infectivity, but extended the median lethal time (LT50). Taken together, these results indicate that Bm91 is not essential for viral propagation in vitro, but absence of the gene may affect the virulence of ODVs in silkworm larvae.
authors: School of Food and Biological Engineering, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
authors: Qi
authors: Q
authors: Tang
authors: Guohui
authors: G
authors: Li
authors: Qin
authors: Q
authors: Yao
authors: Liang
authors: L
authors: Chen
authors: Peng
authors: P
authors: Lv
authors: Chaoqun
authors: C
authors: Lian
authors: Keping
authors: K
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000821:virology*; D055786:Gene Knockout Techniques; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D014759:Viral Envelope Proteins