title: Effect of vegetation on the presence and genetic diversity of Bacillus thuringiensis in soil.
abstract: Bacillus thuringiensis isolates were obtained from soil samples collected at different sites located in the same region but with different vegetation. The sites showed different frequencies of B. thuringiensis, depending on the type of vegetation. Strains of B. thuringiensis were found to be less common in samples of riparian forest soil than in soil of other types of vegetation. The rate of occurrence of B. thuringiensis in the samples also varied according to the vegetation. These results show that whenever this bacterium was found, it showed a high rate of occurrence, indicating that this species could be better adapted to using soil as a reservoir than other Bacillus species. The presence of cry genes was analyzed by polymerase chain reaction, and genes that exhibited activity against Diptera species were the most commonly found. The isolates obtained were characterized by random amplified polymorphic DNA, and 50% were clustered into clonal groups. These results demonstrated the possible occurrence of a high number of genetically similar strains when samples are collected from the same region, even if they are from locations with different vegetation.
authors: Departamento de Biologia Geral, Universidade Estadual de Londrina, CP6001, 86051-990, Londrina/PR, Brazil.
authors: Ana Paula Scaramal
authors: AP
authors: Ricieto
authors: Fernanda Aparecida Pires
authors: FA
authors: Fazion
authors: Celso Duarte
authors: CD
authors: Carvalho Filho
authors: Laurival Antonio
authors: LA
authors: Vilas-Boas
authors: Gislayne Trindade
authors: GT
authors: Vilas-Bôas
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D017753:Ecosystem; D004731:Endotoxins / Q000235:genetics; D014644:Genetic Variation; D006460:Hemolysin Proteins / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D010944:Plants / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis