title: Capillary electrophoresis-mass spectrometry for Peptide analysis: target-based approaches and proteomics/peptidomics strategies.
abstract: In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by the presentation of two different strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligopeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive dipeptide in a legume plant. The second one is a shotgun-like methodology used for proteomic analysis. Particularly, CE-MS using a time-of-flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics.
authors: Laboratory of Foodomics, CIAL, CSIC, Madrid, Spain.
authors: Carolina
authors: C
authors: Simó
authors: Alejandro
authors: A
authors: Cifuentes
authors: Václav
authors: V
authors: Kašička
mesh_terms: D004151:Dipeptides / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D057230:Limit of Detection; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D059748:Proteolysis; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry* / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry; D031306:Vicia / Q000737:chemistry
chemical_list: D004151:Dipeptides; D010446:Peptide Fragments; D010940:Plant Proteins; D020543:Proteome; C503744:gamma-glutamyl-S-ethenyl-cysteine