title: Visualization of fas-mediated death-inducing signaling complex formation by immunoprecipitation.
abstract: The prototypical death receptor Fas (also known as CD95 or Apo-1) plays an essential role in the maintenance of lymphocyte homeostasis. Propagation of cell death through Fas relies on the formation of a multiprotein complex at the receptor level known as the death-inducing signaling complex (DISC). Here, we describe an immunoprecipitation-based protocol to study DISC assembly in activated human T lymphocytes. This procedure is a useful tool to visualize proteins associated with Fas.
authors: INSERM UMR_S 1014, Hôpital Paul Brousse, Villejuif, France.
authors: Nelia
authors: N
authors: Cordeiro
authors: Nicolas
authors: N
authors: Bidère
mesh_terms: D018929:Cell Culture Techniques; D003101:Collodion / Q000737:chemistry; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation / Q000379:methods*; D008213:Lymphocyte Activation; D008567:Membranes, Artificial; D057054:Molecular Imaging / Q000379:methods*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D053418:Death Domain Receptor Signaling Adaptor Proteins; D008567:Membranes, Artificial; D019014:fas Receptor; D003101:Collodion