title: Antiobesity activity of Vigna nakashimae extract in high-fat diet-induced obesity.
abstract: In this study, we evaluated the antiobesity effects of Vigna nakashimae (VN) extract and elucidated the underlying mechanisms. VN extract suppressed adipocyte differentiation and significantly attenuated the expression of adipogenic genes in 3T3-L1 cells. It decreased the expression of peroxisome proliferator-activated receptor γ (PPARγ) and its target genes in fully differentiated 3T3-L1 cells. Moreover, it enhanced the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl CoA carboxylase (ACC), and increased the expression of fatty acid oxidation genes. In high-fat diet (HFD) fed mice, VN extract suppressed HFD-induced increases in body weight, epididymal fat tissue weight, and hepatic lipid levels, and decreased the plasma levels of triacylglycerols, fatty acid, total cholesterol, and inflammatory cytokines. Consistently with in vitro study results, VN extract prevented HFD-induced increases in the expression of PPARγ and its target genes, and restored the decrease in the phosphorylation of AMPK and ACC in epididymal fat and liver tissues. These findings suggest that Vigna nakashimae prevents obesity through suppression of PPARγ expression and activation of AMPK, and that it might be a useful dietary supplement for the prevention of obesity.
authors: School of Korean Medicine, Pusan National University, Gyeongnam, South Korea.
authors: Yonghae
authors: Y
authors: Son
authors: Jeong-Su
authors: JS
authors: Nam
authors: Min-Kyung
authors: MK
authors: Jang
authors: In-Ah
authors: IA
authors: Jung
authors: Su-In
authors: SI
authors: Cho
authors: Myeong-Ho
authors: MH
authors: Jung
mesh_terms: D041721:3T3-L1 Cells; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat; D007887:Fabaceae / Q000737:chemistry*; D005227:Fatty Acids / Q000097:blood; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism / Q000517:prevention & control*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
chemical_list: D019440:Anti-Obesity Agents; D005227:Fatty Acids; D047495:PPAR gamma; D010936:Plant Extracts; D014280:Triglycerides; D002784:Cholesterol; D000103:Acetyl-CoA Carboxylase