title: The regulatory effect of veratric acid on NO production in LPS-stimulated RAW264.7 macrophage cells.
abstract: Veratric acid, a simple benzoic acid derived from plants and fruits, has been reported to have anti-oxidant, anti-inflammation, and blood pressure-lowering effects. This study was designed to evaluate the inhibitory effects of veratric acid on nitric oxide (NO) production in LPS-stimulated RAW264.7 cells. It was found that veratric acid inhibited NO production and inducible nitric oxide synthase (iNOS) expression in LPS-stimulated RAW264.7 cells. The inhibitory effects of veratric acid on the generation of interleukin-6 (IL-6) and interferon-γ (IFN-γ) was determined. Furthermore, veratric acid facilitated the inactivation of glycogen synthase kinase-3β (GSK-3β), STAT-1, and STAT-3 in dose-dependent manner. Notably, NF-κB and members of the mitogen activated protein kinase (MAPK) family including p38, ERK, and JNK were dephosphorylated by veratric acid. These findings suggest that the treatment of veratric acid might be effective in neutralizing the over-expression of NO in inflammatory disorders.
authors: Department of Microbiology, College of Natural Sciences, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Woo-Suk
authors: WS
authors: Choi
authors: Pyung-Gyun
authors: PG
authors: Shin
authors: Jong-Hwan
authors: JH
authors: Lee
authors: Gun-Do
authors: GD
authors: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D010766:Phosphorylation; D014641:Vanillic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; C009333:veratric acid; D009569:Nitric Oxide; D007371:Interferon-gamma; D048049:Extracellular Signal-Regulated MAP Kinases; D014641:Vanillic Acid