title: Early repeated maternal separation induces alterations of hippocampus reelin expression in rats.
abstract: The long-term effects of repeated maternal separation (MS) during early postnatal life on reelin expression in the hippocampus of developing rats were investigated in the present study. MS was carried out by separating Wistar rat pups singly from their mothers for 3 h a day during postnatal days (PND) 2-14. Reelin mRNA and protein levels in the hippocampus were determined using qRT-PCR and Western blotting, at PND 22, PND 60 and PND 90. MS resulted in the loss of body weight in the developing rats, and reelin mRNA and protein levels in the hippocampus generally were down-regulated over the developing period, but the reelin mRNA and protein levels in the hippocampus of 90-day-old male rats were up-regulated. These findings suggest that the long-term effects of MS on the expression levels of hippocampal reelin mRNA and protein depends on the age at which the stressed rats' brains were collected; reelin had important implications for the maternal-neonate interaction needed for normal brain development. In conclusion, repeated MS occurring during early postnatal life may cause the alterations of hippocampal reelin expression with the increasing age of developing rats.
authors: Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
authors: Jianlong
authors: J
authors: Zhang
authors: Lina
authors: L
authors: Qin
authors: Hu
authors: H
authors: Zhao
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000235:genetics* / Q000523:psychology; D001835:Body Weight; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008426:Maternal Deprivation; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D012737:Sex Factors; D013315:Stress, Psychological
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000091042:Reelin Protein; C000717129:Reln protein, rat; D012697:Serine Endopeptidases
references: Neurosci Lett. 2009 Aug 28;460(2):170-4
references: 19477232
references: Neurogastroenterol Motil. 2003 Dec;15(6):679-86
references: 14651604
references: Exp Brain Res. 2004 Jan;154(2):183-91
references: 14557907
references: Behav Brain Res. 2011 Feb 2;217(1):142-7
references: 20974192
references: J Neurophysiol. 2007 Mar;97(3):2312-21
references: 17229826
references: Neuron. 2005 Aug 18;47(4):471-3
references: 16102527
references: Neurosci Biobehav Rev. 2009 Apr;33(4):573-85
references: 18817811
references: Hippocampus. 2006;16(3):225-32
references: 16411182
references: Biol Psychiatry. 2000 Oct 15;48(8):755-65
references: 11063972
references: Psychoneuroendocrinology. 2010 Oct;35(9):1374-87
references: 20452127
references: Neurosci Biobehav Rev. 2009 Apr;33(4):560-72
references: 18845182
references: Annu Rev Neurosci. 2001;24:1161-92
references: 11520931
references: Metab Brain Dis. 2007 Jun;22(2):183-95
references: 17468977
references: Nat Neurosci. 2000 Aug;3(8):799-806
references: 10903573
references: JAMA. 2000 Aug 2;284(5):592-7
references: 10918705
references: J Neurosci. 2003 Jul 16;23(15):6272-9
references: 12867512
references: N Engl J Med. 2008 Jul 3;359(1):61-73
references: 18596274
references: Ann N Y Acad Sci. 2004 Dec;1032:63-84
references: 15677396
references: Nat Genet. 2000 Sep;26(1):93-6
references: 10973257
references: J Neurosci. 2006 Dec 13;26(50):12943-55
references: 17167084
references: Brain Res Bull. 2003 Dec 15;62(2):85-91
references: 14638381
references: Attach Hum Dev. 2000 Apr;2(1):23-47
references: 11707891
references: Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3480-5
references: 16484373
references: J Comp Neurol. 1991 Nov 15;313(3):486-93
references: 1770172
references: Brain Res. 2002 Sep 20;950(1-2):52-63
references: 12231228
references: Behav Brain Res. 2007 Feb 12;177(1):142-9
references: 17141885
references: Prog Neurobiol. 2007 Mar;81(4):197-217
references: 17350153
references: Neuron. 2004 Jan 8;41(1):71-84
references: 14715136
references: Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3221-6
references: 9501244
references: Nucleic Acids Res. 2001 May 1;29(9):e45
references: 11328886
references: Brain Res. 2003 Jun 13;975(1-2):22-36
references: 12763590
references: Biol Psychiatry. 2001 Jun 15;49(12):1023-39
references: 11430844
references: J Neurochem. 2002 Aug;82(4):998-1002
references: 12358805
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Nature. 1995 Apr 20;374(6524):719-23
references: 7715726
references: Depress Anxiety. 2002;15(3):126-47
references: 12001181
references: Neurosci Lett. 2007 Jun 27;421(2):115-20
references: 17566656
references: Physiol Behav. 1996 Jan;59(1):99-107
references: 8848498
references: Gut. 2004 Apr;53(4):501-6
references: 15016743
references: Neuron. 2005 Aug 18;47(4):567-79
references: 16102539