title: The inhibitory effect of roasted licorice extract on human metastatic breast cancer cell-induced bone destruction.
abstract: The aim of this study was to determine whether the ethanol extract of roasted licorice (rLE) could inhibit breast cancer-mediated bone destruction. rLE treatment reduced the viability of MDA-MB-231 human metastatic breast cancer cells but did not show any cytotoxicity in hFOB1.19 human osteoblastic cells and murine bone marrow-derived macrophages (BMMs). rLE inhibited expression and secretion of receptor activator of nuclear factor κB ligand (RANKL) as well as the mRNA and protein expression of cyclooxygenase-2 in osteoblastic cells exposed to the conditioned medium of breast cancer cells. rLE dramatically inhibited RANKL-induced osteoclastogenesis in BMMs, thereby reducing osteoclast-mediated pit formation. Moreover, treatment with licochalcone A and isoliquiritigenin as the active components, whose contents are increased by the roasting process, remarkably suppressed RANKL-induced osteoclast formation in BMMs, respectively. Furthermore, orally administered rLE substantially blocked tumor growth and bone destruction in mice inoculated with breast cancer cells in the tibiae. Serum levels of tartrate-resistant acid phosphatase and C-terminal cross-linking telopeptide of type I collagen and trabecular bone morphometric parameters were reversed to almost the same levels as the control mice by the rLE treatment. In conclusion, rLE may be a beneficial agent for preventing and treating bone destruction in patients with breast cancer.
authors: Department of Oral Biology and Oral Cancer Research Institute, Yonsei University College of Dentistry, Seoul, 120-752, Korea; Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 120-749, Korea.
authors: Sun Kyoung
authors: SK
authors: Lee
authors: Kwang-Kyun
authors: KK
authors: Park
authors: Jung Han Yoon
authors: JH
authors: Park
authors: Soon Sung
authors: SS
authors: Lim
authors: Won-Yoon
authors: WY
authors: Chung
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology / Q000556:secondary; D001862:Bone Resorption / Q000188:drug therapy / Q000473:pathology*; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D047188:Chalcones / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005260:Female; D006035:Glycyrrhiza / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009362:Neoplasm Metastasis; D010006:Osteoblasts / Q000187:drug effects*; D010010:Osteoclasts / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D053245:RANK Ligand / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D047188:Chalcones; D007527:Isoenzymes; D010936:Plant Extracts; D053245:RANK Ligand; C040920:isoliquiritigenin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; C070840:licochalcone A
keywords: RANKL-induced osteoclastogenesis; bone destruction; breast cancer; roasted licorice extract