title: Two-dimensional nuclear magnetic resonance structure determination module for introductory biochemistry: synthesis and structural characterization of lyso-glycerophospholipids.
abstract: In this laboratory module, introductory biochemistry students are exposed to two-dimensional (1) H-nuclear magnetic resonance of glycerophospholipids (GPLs). Working in groups of three, students enzymatically synthesized and purified a variety of 2-acyl lyso GPLs. The structure of the 2-acyl lyso GPL was verified using (1) H-correlation spectroscopy. Students scored significantly higher on an assessment of NMR knowledge after having participated in this lab module and in comparison to a similar cohort who did not participate. Inaddition, student confidence in their NMR knowledge and abilities increased 62% following the module and correlated with their ability to apply their NMR knowledge. Based on these results, the laboratory module was very effective at providing students with a more extensive understanding of the underlying concepts of NMR as a tool for structural determination.
authors: Department of Chemistry, Vassar College, 124 Raymond Avenue, Poughkeepsie, New York, 12604, USA. tegarrett@vassar.edu
authors: Teresa A
authors: TA
authors: Garrett
authors: Rebecca L
authors: RL
authors: Rose
authors: Sidney M
authors: SM
authors: Bell
mesh_terms: D000215:Acylation; D001671:Biochemistry / Q000193:education*; D002855:Chromatography, Thin Layer; D020404:Glycerophospholipids / Q000138:chemical synthesis* / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008246:Lysophospholipids / Q000138:chemical synthesis* / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons
chemical_list: D020404:Glycerophospholipids; D008246:Lysophospholipids; D011522:Protons