title: MicroRNA-338-3p promotes differentiation of mDPC6T into odontoblast-like cells by targeting Runx2.
abstract: Odontoblasts are terminally differentiated cells that play a vital role in dentinogenesis. The differentiation of odontoblasts is regulated by a variety of genetic and epigenetic mechanisms. Our previous microRNA microarray studies verified that miR-338-3p was up-regulated during odontoblast differentiation. The purpose of this study was to determine the function of miR-338-3p during odontoblast differentiation. The upregulation of miR-338-3p expression during odontoblast differentiation was validated by qRT-PCR. Odontoblast differentiation was enhanced after over-expression of miR-338-3p, while a loss of function approach using a miR-338-3p inhibitor impaired odontoblast differentiation. Bioinformatic analysis identified Runx2 as a potential target of miR-338-3p. Overexpression of miR-338-3p caused a decreased in the expression of Runx2 at both mRNA and protein levels, while Runx2 expression increased after treatment with miR-338-3p inhibitors. Furthermore, the activity of a luciferase reporter plasmid containing the 3'-UTR of Runx2 was significantly suppressed by ectopic expression of miR-338-3p. These results suggested that miR-338-3p promotes odontoblast differentiation through targeting Runx2.
authors: State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: Qin
authors: Q
authors: Sun
authors: Huan
authors: H
authors: Liu
authors: Heng
authors: H
authors: Lin
authors: Guohua
authors: G
authors: Yuan
authors: Lu
authors: L
authors: Zhang
authors: Zhi
authors: Z
authors: Chen
mesh_terms: D020413:3' Untranslated Regions; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D009804:Odontoblasts / Q000166:cytology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D050659:Core Binding Factor Alpha 1 Subunit; C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; C532050:MIRN338 microRNA, human; D035683:MicroRNAs; D010750:Phosphoproteins; C493709:RUNX2 protein, human; D000469:Alkaline Phosphatase
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