title: Chlamydophila pneumoniae re-infection triggers the production of IL-17A and IL-17E, important regulators of airway inflammation.
abstract: OBJECTIVE
  Investigation of the effects of interleukin (IL)-17 cytokines in Chlamydophila pneumoniae-infected mice.
  
  
  METHODS
  Mice were infected with C. pneumoniae once or three times and the expression of IL-17 cytokines was followed by RT qPCR from day 1 to day 28 after infection and re-infection. After the treatment of mice with anti-IL-17A, ELISA was used to detect the differences in cytokine and chemokine production. The number and phenotype of the IL-17A-producing cells were determined by ELISPOT.
  
  
  RESULTS
  Chlamydophila pneumoniae induced IL-17A and IL-17F from day 2 after infection, and their levels remained elevated on day 28. The expression of IL-17C, IL-17D and IL-17E mRNA did not change significantly in response to a single infection. The in vivo neutralization of IL-17A resulted in a higher C. pneumoniae burden in the mouse lungs, a decreased cell influx, and diminished chemokine levels. The phenotype of IL-17A-producing cells was CD4(+). The re-infection of mice led to an increased expression of IL-17E mRNA.
  
  
  CONCLUSIONS
  These results facilitate an understanding of the early inflammatory response after C. pneumoniae infection and suggest that C. pneumoniae re-infection induces the production of a high amount of IL-17E, which has an important role in the pathogenesis of allergic pulmonary diseases.
authors: Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.
authors: Tímea
authors: T
authors: Mosolygó
authors: József
authors: J
authors: Korcsik
authors: Emese Petra
authors: EP
authors: Balogh
authors: Ildikó
authors: I
authors: Faludi
authors: Dezső P
authors: DP
authors: Virók
authors: Valéria
authors: V
authors: Endrész
authors: Katalin
authors: K
authors: Burián
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D023521:Chlamydophila Infections / Q000276:immunology*; D016993:Chlamydophila pneumoniae; D005260:Female; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011014:Pneumonia / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D020381:Interleukin-17; D012333:RNA, Messenger
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