title: The influence of bupivacaine on the whole blood chemiluminescence in laboring women--a preliminary report.
abstract: OBJECTIVE
  Local anesthetics are able to inhibit inflammatory phenomena. The influence of bupivacaine broadly applied in obstetrical anesthesia on the reactive oxygen species (ROS) production is a matter of controversy. The study was aimed at elucidation of the influence of racemic bupivacaine on the opsonized zymosan (OZ) stimulated-peripheral blood chemiluminescence (CL) in laboring women, reflecting the reactive oxygen species (ROS) production associated with phagocytosis.
  
  
  METHODS
  Blood samples drawn from 8 healthy parturients in active spontaneous labor and from 5 healthy non-pregnant controls were incubated with the 0.3, 30, and 3000 microM bupivacaine concentrations and then luminol-dependent OZ-stimulated whole blood CL was assessed.
  
  
  RESULTS
  Bupivacaine depressed CL; however the inhibitory effect was significant only at the highest, clinically irrelevant concentration (3000 microM), in parturients being comparable to that observed in non-pregnant women.
  
  
  CONCLUSIONS
  Bupivacaine at clinically relevant concentrations does not influence ROS production accompanying phagocytosis in peripheral blood of laboring women. The effect is comparable in parturients and non-pregnant controls.
authors: Department of Experimental Anesthesiology, Poznań University of Medical Sciences, Poznań, Poland. hbillert@op.pl
authors: Hanna
authors: H
authors: Billert
authors: Ewa
authors: E
authors: Bednarek
authors: Aleksandra
authors: A
authors: Szumczyk
authors: Michał
authors: M
authors: Gaca
authors: Grzegorz
authors: G
authors: Breborowicz
mesh_terms: D000328:Adult; D000773:Anesthesia, Obstetrical / Q000379:methods; D000779:Anesthetics, Local / Q000494:pharmacology*; D002045:Bupivacaine / Q000494:pharmacology*; D005260:Female; D006801:Humans; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D007744:Obstetric Labor Complications / Q000097:blood / Q000188:drug therapy*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000097:blood*; D012016:Reference Values; D055815:Young Adult
chemical_list: D000779:Anesthetics, Local; D017382:Reactive Oxygen Species; D002045:Bupivacaine