title: Fabrication of chitosan nanoparticles with aggregation-induced emission characteristics and their applications in long-term live cell imaging.
abstract: Chitosan with tetraphenylethene pendants (TPE-CS) are synthesized by reaction between amine and isothiocyanate groups of chitosan and tetraphenylethene (TPE), respectively. Nanoparticles of TPE-CS (TPE-CS NPs) are fabricated by ionic gelation method. The NPs are uniform in size, spherical in shape, monodispersed, and positive in surface charge. The suspension of TPE-CS NPs emits strong blue fluorescence under photoexcitation due to the aggregation-induced emission characteristics of the TPE moieties. The NPs can be internalized into cytoplasm through endocytosis pathway and retain inside the live cells to image the cells. Cytotoxicity assay reveals that TPE-CS NPs are cytocompatible and thus can be used for long-term live cell imaging.
authors: Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Min
authors: M
authors: Li
authors: Yuning
authors: Y
authors: Hong
authors: Zhengke
authors: Z
authors: Wang
authors: Sijie
authors: S
authors: Chen
authors: Meng
authors: M
authors: Gao
authors: Ryan T K
authors: RT
authors: Kwok
authors: Wei
authors: W
authors: Qin
authors: Jacky W Y
authors: JW
authors: Lam
authors: Qichang
authors: Q
authors: Zheng
authors: Ben Zhong
authors: BZ
authors: Tang
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D008027:Light; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D048271:Chitosan