title: Crystallization and preliminary diffraction analysis of the catalytic domain of major extracellular endoglucanase from Xanthomonas campestris pv. campestris.
abstract: Cellulases, such as endoglucanases, exoglucanases and β-glucosidases, are important enzymes used in the process of enzymatic hydrolysis of plant biomass. The bacteria Xanthomonas campestris pv. campestris expresses a large number of hydrolases and the major endoglucanase (XccEG), a member of glycoside hydrolase family 5 (GH5), is the most strongly secreted extracellularly. In this work, the native XccEG was purified from the extracellular extract and crystallization assays were performed on its catalytic domain. A complete data set was collected on an in-house X-ray source. The crystal diffracted to 2.7 Å resolution and belonged to space group C2, with unit-cell parameters a = 174.66, b = 141.53, c = 108.00 Å, β = 110.49°. The Matthews coefficient suggests a solvent content of 70.1% and the presence of four protein subunits in the asymmetric unit.
authors: Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador Saocarlense 400, São Carlos-SP 13560-970, Brazil.
authors: Flávio R
authors: FR
authors: Rosseto
authors: Ana C
authors: AC
authors: Puhl
authors: Maxuel O
authors: MO
authors: Andrade
authors: Igor
authors: I
authors: Polikarpov
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D002480:Cellulase / Q000737:chemistry* / Q000302:isolation & purification; D011232:Chemical Precipitation; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000201:enzymology*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D014961:X-Ray Diffraction; D016959:Xanthomonas campestris / Q000201:enzymology*
chemical_list: D021382:Protein Sorting Signals; D002480:Cellulase
keywords: Xanthomonas campestris pv. campestris; cellulases; endoglucanases
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