title: Purification and fermentation in vitro of sesaminol triglucoside from sesame cake by human intestinal microbiota.
abstract: Sesaminol triglucoside (STG), the most abundant lignan glycoside existing in sesame cake/meal, has exhibited various biological activities. However, little information about its in vitro fermentation with intestinal microbiota is available. Therefore, the effect of STG from sesame cake on the fermentation of human fecal microbiota was evaluated. First, high-purity STG was successfully prepared from defatted sesame cake by extraction with 80% ethanol and simple purification procedures of polyamide column chromatography and Toyopearl HW-40S column chromatography. Then the influence of STG on intestinal microbiota was conducted by monitoring bacterial populations and analyzing the concentrations of short-chain fatty acids (SCFA). We found that STG could significantly induce an increase in numbers of  Lactobacillus - Enterococcus  group and  Bifidobacterium  in fermentation in vitro with human fecal microbiota, while it did not stimulate the bacterial growth of  Eubacterium rectale - Clostridium coccoides  group,  Clostridium histolyticum  group, and  Bacteroides - Prevotella  group. Furthermore, it was found that concentrations of formic, acetic, propionic, and butyric acids in STG culture increased significantly during the fermentation, and its total SCFA concentration was relatively higher than those of the control and glucose cultures at 6 and 12 h fermentation. Our findings provided further evidence for the importance of human intestinal bacteria in the bioactivity of STG and its metabolites in the maintenance of human health.
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors: Xiuling
authors: X
authors: Zhu
authors: Xin
authors: X
authors: Zhang
authors: Yongkang
authors: Y
authors: Sun
authors: Di
authors: D
authors: Su
authors: Yi
authors: Y
authors: Sun
authors: Bing
authors: B
authors: Hu
authors: Xiaoxiong
authors: X
authors: Zeng
mesh_terms: D000328:Adult; D005260:Female; D005285:Fermentation; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology*; D008297:Male; D013058:Mass Spectrometry; D064307:Microbiota; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D031685:Sesamum / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005960:Glucosides; D010936:Plant Extracts; C518861:sesaminol triglucoside