title: Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities.
abstract: Isolation of reference DNA templates for quantitative real-time PCR assays is an expensive, labor-intensive and time-consuming process if they are not readily available. Two artificial DNA templates with multiple probe sites were designed for quantifying methanogens and their 10 subgroups, based on the methyl coenzyme M reductase gene (mcrA). Their standards were comparable to each other. PCR amplification efficiencies (cycle vs. cumulative fluorescence) of the artificial DNAs were also comparable to those of the observed methanogen groups from anaerobic digesters. The artificial templates can be alternatives to the actual references.
authors: Department of Environmental Science and Engineering, Ewha Womans University, Seodaemun-gu, Seoul, Korea.
authors: Tae Gwan
authors: TG
authors: Kim
authors: Taewoo
authors: T
authors: Yi
authors: Kyung-Suk
authors: KS
authors: Cho
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D019605:Euryarchaeota / Q000235:genetics*; D017014:Methanobacterium / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D017931:DNA Primers; D012722:Sewage; D010088:Oxidoreductases; C021572:methyl coenzyme M reductase