title: Highly efficient virus-induced gene silencing in apple and soybean by apple latent spherical virus vector and biolistic inoculation.
abstract: Virus-induced gene silencing (VIGS) is an effective tool for the analysis of the gene function in plants within a short time. However, in woody fruit tree like apple, some of Solanum crops, and soybean, it is generally difficult to inoculate virus vector by conventional inoculation methods. Here, we show efficient VIGS in apple and soybean by Apple latent spherical virus (ALSV) vector and biolistic inoculation. The plants inoculated with ALSV vectors by particle bombardment showed uniform silenced phenotypes of target genes within 2-3 weeks post inoculation.
authors: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors: Noriko
authors: N
authors: Yamagishi
authors: Nobuyuki
authors: N
authors: Yoshikawa
mesh_terms: D019470:Biolistics; D027465:Chenopodium quinoa / Q000821:virology; D003001:Cloning, Molecular; D055785:Gene Knockdown Techniques / Q000379:methods*; D005822:Genetic Vectors; D018525:Germination; D027845:Malus / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D010849:Picornaviridae / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000821:virology; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000821:virology; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development / Q000821:virology
chemical_list: D018749:RNA, Plant