title: Strains of Pseudomonas syringae pv. syringae from pea are phylogenetically and pathogenically diverse.
abstract: Pseudomonas syringae pv. syringae causes extensive yield losses in the pea crop worldwide, although there is little information on its host specialization and its interactions with pea. A collection of 88 putative P. syringae pv. syringae strains (including 39 strains isolated from pea) was characterized by repetitive polymerase chain reaction (rep-PCR), multilocus sequence typing (MLST), and syrB amplification and evaluated for pathogenicity and virulence. rep-PCR data grouped the strains from pea into two groups (1B and 1C) together with strains from other hosts; a third group (1A) was formed exclusively with strains isolated from non-legume species. MLST data included all strains from pea in the genomospecies 1 of P. syringae pathovars defined in previous studies; they were distributed in the same three groups defined by rep-PCR. The inoculations performed in two pea cultivars showed that P. syringae pv. syringae strains from groups 1A and 1C were less virulent than strains from group 1B, suggesting a possible pathogenic specialization in this group. This study shows the existence of genetically and pathogenically distinct P. syringae pv. syringae strain groups from pea, which will be useful for the diagnostic and epidemiology of this pathogen and for disease resistance breeding.
authors: Instituto Tecnológico Agrario, Consejería de Agricultura y Ganadería de la Junta de Castilla y León Ctra, Burgos, Spain.
authors: Alberto
authors: A
authors: Martín-Sanz
authors: Marcelino Pérez
authors: MP
authors: de la Vega
authors: Jesús
authors: J
authors: Murillo
authors: Constantino
authors: C
authors: Caminero
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D018532:Pisum sativum / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial