title: A direct comparison of expression profiles of adhesion molecules on naïve T cells between cord blood and steady-state bone marrow grafts of healthy donors.
abstract: BACKGROUND
  We compared the profiles of adhesion molecule expression on naïve T cells between umbilical cord blood (UCB) and steady-state bone marrow (SS-BM) grafts.
  
  
  METHODS
  The expressions of 4 adhesion molecules, including very late antigen 4 (VLA-4), intercellular adhesion molecule-1 (ICAM-1), L-selectin, and lymophocyte function-associated antigen-1 (LFA-1) on naïve T cells in UCB (n = 25) and SS-BM (n = 10) were analyzed using flow cytometry.
  
  
  RESULTS
  The expressions of ICAM-1 and L-selectin on CD4(+) T cells and CD8(+) T cells in UCB were significantly lower than those on SS-BM (P < .05 for all). The expressions of VLA-4 and LFA-1 on CD8(+) T cells in UCB were significantly lower than those of SS-BM (P = .002 and .047, respectively). Compared with SS-BM, we observed lower expression of ICAM-1 on naïve CD4(+) and CD8(+) T cells in UCB (P < .001 for all). The percentages of interferon (IFN)-γ positive cells among naïve CD4(+) and CD8(+) T-cell subsets were significantly lower in UCB, leading to ready polarization of naïve UCB T cells from a Th1 to Th2 phenotype versus those on SS-BM.
  
  
  CONCLUSIONS
  Our results among UCB suggested lower intensities of ICAM-1 expression on naïve T cells and their easier polarization from Th1 to Th2 elements.
authors: Department of Hematology, Third Affiliated Hospital of Hebei Medical University, Shijiazhuang, China. happys.ok@163.com
authors: L-X
authors: LX
authors: Sun
authors: Y-Q
authors: YQ
authors: Li
authors: X-N
authors: XN
authors: Song
authors: J
authors: J
authors: Jiang
authors: Y-J
authors: YJ
authors: Chang
mesh_terms: D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000379:methods*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002465:Cell Movement; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006086:Graft vs Host Disease; D006801:Humans; D039041:Integrin alpha4beta1 / Q000097:blood*; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D007371:Interferon-gamma / Q000378:metabolism; D019041:L-Selectin / Q000097:blood*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D018417:Th1 Cells / Q000166:cytology; D018418:Th2 Cells / Q000166:cytology; D055815:Young Adult
chemical_list: D039041:Integrin alpha4beta1; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D007371:Interferon-gamma