title: Expression and function of scalloped during Drosophila development.
abstract: BACKGROUND
  The scalloped (sd) and vestigial (vg) genes function together in Drosophila wing development. Little is known about sd protein (SD) expression during development, or whether sd and vg interact in other developing tissues. To begin to address these questions, we generated an anti-SD antibody.
  
  
  RESULTS
  During embryogenesis, SD is expressed in both central and peripheral nervous systems, and the musculature. SD is also expressed in developing flight appendages. Despite SD expression herein, the peripheral nervous system, musculature, and dorsal limb primordia appeared generally normal in the absence of sd function. SD is also expressed in subsets of ventral nerve cord cells, including neuroblast 1-2 descendants and ventral unpaired median motor neurons (mVUMs). While sd function is not required to specify these neurons, it is necessary for the correct innervation of somatic muscles by the mVUMs. We also show that SD and vg protein (VG) are co-expressed in overlapping and distinctive subsets of cells in embryonic and larval tissues.
  
  
  CONCLUSIONS
  We describe the full breadth of SD expression during Drosophila embryogenesis, and identify a requirement for sd function in a subset of motor neurons. This work provides the necessary foundation for functional studies regarding the roles of sd during Drosophila development.
authors: Department of Biology, Dickinson College, Carlisle, Pennsylvania 17013, USA. gussk@dickinson.edu
authors: Kirsten A
authors: KA
authors: Guss
authors: Michael
authors: M
authors: Benson
authors: Nicholas
authors: N
authors: Gubitosi
authors: Karrie
authors: K
authors: Brondell
authors: Kendal
authors: K
authors: Broadie
authors: James B
authors: JB
authors: Skeath
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D060227:Imaginal Discs / Q000196:embryology / Q000378:metabolism; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D017933:Peripheral Nervous System / Q000196:embryology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D014157:Transcription Factors; C087984:sd protein, Drosophila
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