title: Rhodamine-based Cu2+-selective fluorosensor: synthesis, mechanism, and application in living cells.
abstract: A rhodamine B-based fluorescence probe (1) for the sensitive and selective detection of Cu(2+) ion has been designed and synthesized using pyridine moiety. The optical properties of this compound have been investigated in acetonitrile-water binary solution (7:3 v/v). Compound 1 is found to be an excellent sensor for a biologically/physiologically very important transition metal ion (Cu(2+)) using only the two very different modes of measurements (absorption and emission); one case displayed intensity enhancement whereas in other case showed intensity depletion (quenching). A mechanistic investigation has been performed to explore the static nature of quenching process. The sensor has been found to be very effective in sensing Cu(2+) ion inside living cells also.
authors: Department of Chemistry, National Institute of Technology, Durgapur, WB, India.
authors: Anindita
authors: A
authors: Sikdar
authors: Swapnadip
authors: S
authors: Roy
authors: Kakali
authors: K
authors: Haldar
authors: Soma
authors: S
authors: Sarkar
authors: Sujit S
authors: SS
authors: Panja
mesh_terms: D002470:Cell Survival; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D060326:Chemistry Techniques, Synthetic; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D004926:Escherichia coli / Q000166:cytology; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D012545:Schiff Bases; D003300:Copper; C029773:rhodamine B
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