title: Rhamnogalacturonan from Ilex paraguariensis: a potential adjuvant in sepsis treatment.
abstract: The present study evaluated the anti-inflammatory activity of a polysaccharide from maté, using a clinically relevant model of sepsis induced by cecal ligation and puncture (CLP). A polysaccharide from maté (SPI) was obtained from aqueous extraction followed by fractionation, being identified as a rhamnogalacturonan with a main chain of →4)-6-OMe-α-D-GalpA-(1→ groups, interrupted by α-L-Rhap units, substituted by a type I arabinogalactan. SPI was tested against induced-polymicrobial sepsis, at doses of 3, 7 and 10 mg/kg. Via oral administration, SPI prevented the late mortality of infected mice by a rate of 60% at 10 mg/kg, in comparison with untreated mice Dexamethasone, used as positive control, was slightly less effective, with an overall survival rate of 16.7% of mice at the end of the observation period. SPI also affected neutrophil influx, avoiding its accumulation in lungs, and significantly decreased tissue expression of iNOS and COX-2. In this context, maté is a potential nutraceutical, and its polysaccharide a promising adjuvant for sepsis treatment, being consumed as tea-like beverages with no related adverse effects.
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba, PR, Brazil.
authors: Nessana
authors: N
authors: Dartora
authors: Lauro M
authors: LM
authors: de Souza
authors: Simone M M
authors: SM
authors: Paiva
authors: Camila T
authors: CT
authors: Scoparo
authors: Marcello
authors: M
authors: Iacomini
authors: Philip A J
authors: PA
authors: Gorin
authors: Yanna D
authors: YD
authors: Rattmann
authors: Guilherme L
authors: GL
authors: Sassaki
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D030019:Ilex paraguariensis / Q000737:chemistry*; D008026:Ligation / Q000009:adverse effects; D008297:Male; D008745:Methylation; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011677:Punctures / Q000009:adverse effects; D018805:Sepsis / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology / Q000276:immunology; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D010368:Pectins; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2