title: Differential expression of VGLUT1 or VGLUT2 in the trigeminothalamic or trigeminocerebellar projection neurons in the rat.
abstract: The vesicular glutamate transporters, VGLUT1 and VGLUT2, reportedly display complementary distribution in the rat brain. However, co-expression of them in single neurons has been reported in some brain areas. We previously found co-expression of VGLUT1 and VGLUT2 mRNAs in a number of single neurons in the principal sensory trigeminal nucleus (Vp) of the adult rat; the majority of these neurons sent their axons to the thalamic regions around the posteromedial ventral nucleus (VPM) and the posterior nuclei (Po). It is well known that trigeminothalamic (T-T) projection fibers arise not only from the Vp but also from the spinal trigeminal nucleus (Vsp), and that trigeminocerebellar (T-C) projection fibers take their origins from both of the Vp and Vsp. Thus, in the present study, we examined the expression of VGLUT1 and VGLUT2 in Vp and Vsp neurons that sent their axons to the VPM/Po regions or the cortical regions of the cerebellum. For this purpose, we combined fluorescence in situ hybridization (FISH) histochemistry with retrograde tract-tracing; immunofluorescence histochemistry was also combined with anterograde tract-tracing. The results indicate that glutamatergic Vsp neurons sending their axons to the cerebellar cortical regions mainly express VGLUT1, whereas glutamatergic Vsp neurons sending their axons to the thalamic regions express VGLUT2. The present data, in combination with those of our previous study, indicate that glutamatergic Vp neurons projecting to the cerebellar cortical regions express mainly VGLUT1, whereas the majority of glutamatergic Vp neurons projecting to the thalamus co-express VGLUT1 and VGLUT2.
authors: Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, 710032, People's Republic of China.
authors: Shun-Nan
authors: SN
authors: Ge
authors: Zhi-Hong
authors: ZH
authors: Li
authors: Jun
authors: J
authors: Tang
authors: Yunfei
authors: Y
authors: Ma
authors: Hiroyuki
authors: H
authors: Hioki
authors: Ting
authors: T
authors: Zhang
authors: Ya-Cheng
authors: YC
authors: Lu
authors: Fu-Xing
authors: FX
authors: Zhang
authors: Noboru
authors: N
authors: Mizuno
authors: Takeshi
authors: T
authors: Kaneko
authors: Ying-Ying
authors: YY
authors: Liu
authors: Mandy Siu Yu
authors: MS
authors: Lung
authors: Guo-Dong
authors: GD
authors: Gao
authors: Jin-Lian
authors: JL
authors: Li
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology*; D003911:Dextrans / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D008845:Microinjections; D018613:Microscopy, Confocal; D017348:Microscopy, Electron, Scanning Transmission; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013266:Stilbamidines / Q000378:metabolism; D013788:Thalamus / Q000166:cytology*; D014278:Trigeminal Nuclei / Q000166:cytology*; D050598:Vesicular Glutamate Transport Protein 1 / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D050599:Vesicular Glutamate Transport Protein 2 / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D003911:Dextrans; D012333:RNA, Messenger; D013266:Stilbamidines; D050598:Vesicular Glutamate Transport Protein 1; D050599:Vesicular Glutamate Transport Protein 2; C076397:biotinylated dextran amine; D018698:Glutamic Acid; D001710:Biotin