title: Dynamics of recombinant hG-CSF in transgenic goat: preliminary study in the founder during hormonally induced lactation.
abstract: This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 µg hG-CSF per mL of milk. Two peaks of serum hG-CSF (3,470 and 7,390 pg/mL) were detected in the first half of the lactation. Outside of the lactation, hG-CSF was absent from serum, indicating no ectopic expression. During the treatment to induce lactation, transgenic female presented increased neutrophil and lymphocyte blood counts when compared to nontransgenic female. Despite transient neutrophilia, serum biochemistry profiles indicated normal liver and renal functions. Thus, transgenic goat expressed hG-CSF in quantities sufficient for a commercial bioreactor and remained clinically healthy.
authors: State University of Ceará, Faculty of Veterinary, Fortaleza, Brazil.
authors: Raylene R
authors: RR
authors: Moura
authors: Erica S
authors: ES
authors: Albuquerque
authors: Carlos Henrique S
authors: CH
authors: Melo
authors: Agostinho S
authors: AS
authors: Alcântara-Neto
authors: Ribrio Ivan T P
authors: RI
authors: Batista
authors: Diana Célia S
authors: DC
authors: Nunes-Pinheiro
authors: Alexsandra F
authors: AF
authors: Pereira
authors: Darcio Ítalo A
authors: DÍ
authors: Teixeira
authors: Luciana M
authors: LM
authors: Melo
authors: Irina A
authors: IA
authors: Serova
authors: Lyudmila E
authors: LE
authors: Andreeva
authors: Oleg L
authors: OL
authors: Serov
authors: Vicente José F
authors: VJ
authors: Freitas
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D006728:Hormones / Q000494:pharmacology; D006801:Humans; D007774:Lactation / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D007958:Leukocyte Count; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism
chemical_list: D006728:Hormones; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor