title: Biofilm of Candida albicans from oral cavity of an HIV-infected child: challenge on enamel microhardness.
abstract: OBJECTIVE
  The aim of this study was to analyze the effect of C. albicans on enamel microhardness in vitro.
  
  
  METHODS
  Candida albicans was isolated from the oral mucosa (M) and dentin carious lesion (D) of an HIV+ child. Three groups of 12 enamel blocks each were placed in Petri plates (yeast carbon base agar/1% bovine serum albumin): G1, exposed to biofilm formed by C. albicans from M; G2, exposed to biofilm formed by C. albicans from D; G3, no biofilm. Three enamel blocks from each group were removed on days 3, 5, 8, and 10 after biofilm formation to measure the cross-sectional Knoop microhardness (CSMH) of the enamel areas, exposed and not exposed to biofilm.
  
  
  RESULTS
  CSMH decreased in G1 and G2: in G1 on day 5, and in G2 on day 3 (analysis of variance: P < .05; Mann-Whitney test: P < .05), with a similar mean percentage reduction for both groups.
  
  
  CONCLUSIONS
  Candida albicans can reduce enamel microhardness in vitro.
authors: Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors: Senda
authors: S
authors: Charone
authors: Maristela Barbosa
authors: MB
authors: Portela
authors: Madeleine Souza
authors: MS
authors: das Chagas
authors: Rosangela Maria
authors: RM
authors: de Araújo Soares
authors: Gloria Fernanda Barbosa
authors: GF
authors: de Araújo Castro
mesh_terms: D018441:Biofilms; D002176:Candida albicans / Q000502:physiology*; D002648:Child; D003731:Dental Caries / Q000382:microbiology; D003743:Dental Enamel / Q000382:microbiology* / Q000648:ultrastructure; D003804:Dentin / Q000382:microbiology; D006679:HIV Seropositivity / Q000382:microbiology*; D006244:Hardness; D006801:Humans; D050296:Microbial Viability; D009055:Mouth / Q000382:microbiology*; D009061:Mouth Mucosa / Q000382:microbiology; D009172:Mycology / Q000379:methods; D013997:Time Factors; D017001:Tooth Demineralization / Q000382:microbiology