title: G-protein-coupled receptor interaction with small GTPases.
abstract: In addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as "traffic cops" to coordinate receptor targeting to specific locations or as "signal transducers" to directly mediate receptor signal propagation. As revealed in protein-protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α(2B)- and β(2)-adrenegic receptors with the small GTPases Rab8 and ARF1.
authors: Department of Pharmacology and Toxicology, Georgia Health Sciences University, Augusta, Georgia, USA.
authors: Chunmin
authors: C
authors: Dong
authors: Guangyu
authors: G
authors: Wu
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics / Q000378:metabolism*; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018341:Receptors, Adrenergic, alpha-2; D018343:Receptors, Adrenergic, beta-2; C082377:RAB8A protein, human; D020823:ADP-Ribosylation Factor 1; D020691:rab GTP-Binding Proteins
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