title: Root exudation of phytochemicals in Arabidopsis follows specific patterns that are developmentally programmed and correlate with soil microbial functions.
abstract: Plant roots constantly secrete compounds into the soil to interact with neighboring organisms presumably to gain certain functional advantages at different stages of development. Accordingly, it has been hypothesized that the phytochemical composition present in the root exudates changes over the course of the lifespan of a plant. Here, root exudates of in vitro grown Arabidopsis plants were collected at different developmental stages and analyzed using GC-MS. Principle component analysis revealed that the composition of root exudates varied at each developmental stage. Cumulative secretion levels of sugars and sugar alcohols were higher in early time points and decreased through development. In contrast, the cumulative secretion levels of amino acids and phenolics increased over time. The expression in roots of genes involved in biosynthesis and transportation of compounds represented in the root exudates were consistent with patterns of root exudation. Correlation analyses were performed of the in vitro root exudation patterns with the functional capacity of the rhizosphere microbiome to metabolize these compounds at different developmental stages of Arabidopsis grown in natural soils. Pyrosequencing of rhizosphere mRNA revealed strong correlations (p<0.05) between microbial functional genes involved in the metabolism of carbohydrates, amino acids and secondary metabolites with the corresponding compounds released by the roots at particular stages of plant development. In summary, our results suggest that the root exudation process of phytochemicals follows a developmental pattern that is genetically programmed.
authors: Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, Colorado, United States of America.
authors: Jacqueline M
authors: JM
authors: Chaparro
authors: Dayakar V
authors: DV
authors: Badri
authors: Matthew G
authors: MG
authors: Bakker
authors: Akifumi
authors: A
authors: Sugiyama
authors: Daniel K
authors: DK
authors: Manter
authors: Jorge M
authors: JM
authors: Vivanco
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000254:growth & development; D001483:Base Sequence; D002241:Carbohydrates / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D054892:Metagenome / Q000235:genetics; D008969:Molecular Sequence Data; D053147:Plant Exudates / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D025341:Principal Component Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D013400:Sugar Acids / Q000032:analysis
chemical_list: D002241:Carbohydrates; D053147:Plant Exudates; D013400:Sugar Acids
references: Extremophiles. 2009 Mar;13(2):283-97
references: 19089528
references: Mol Plant Microbe Interact. 2002 Nov;15(11):1173-80
references: 12423023
references: Plant Physiol. 2008 Feb;146(2):762-71
references: 18065561
references: Plant J. 2009 Jan;57(1):171-83
references: 18786000
references: J Biol Chem. 2010 Oct 1;285(40):30654-65
references: 20682788
references: Trends Plant Sci. 2012 Aug;17(8):478-86
references: 22564542
references: Science. 2011 May 27;332(6033):1097-100
references: 21551032
references: Plant Signal Behav. 2009 Nov;4(11):1066-8
references: 20009551
references: Proc Natl Acad Sci U S A. 2006 May 9;103(19):7460-4
references: 16645034
references: Nucleic Acids Res. 2009 Jul;37(Web Server issue):W652-60
references: 19429898
references: New Phytol. 1994 Aug;127(4):617-633
references: 33874382
references: Plant Physiol. 2004 Sep;136(1):2887-94
references: 15347793
references: Plant Physiol. 2009 Dec;151(4):2006-17
references: 19854857
references: Plant Cell. 2001 Jul;13(7):1499-510
references: 11449047
references: Genome Biol. 2009;10(3):R25
references: 19261174
references: Metabolomics. 2010 Sep;6(3):451-465
references: 20676379
references: Plant Cell Environ. 2009 Jun;32(6):666-81
references: 19143988
references: Plant Signal Behav. 2009 Aug;4(8):701-12
references: 19820333
references: Appl Environ Microbiol. 2009 Dec;75(23):7537-41
references: 19801464
references: Appl Microbiol Biotechnol. 2009 Aug;84(1):11-8
references: 19568745
references: Integr Comp Biol. 2010 Nov;50(5):768-77
references: 21558239
references: J Exp Bot. 2009;60(6):1729-42
references: 19342429
references: Microbiology (Reading). 2007 Mar;153(Pt 3):727-736
references: 17322193
references: BMC Bioinformatics. 2008 Sep 19;9:386
references: 18803844
references: Nucleic Acids Res. 2011 Jul;39(Web Server issue):W412-5
references: 21546551
references: Metab Eng. 2003 Apr;5(2):133-49
references: 12850135
references: J Bacteriol. 2005 Jun;187(12):4033-41
references: 15937166
references: Plant Signal Behav. 2009 Aug;4(8):777-80
references: 19820328
references: Mol Plant Microbe Interact. 2012 Feb;25(2):139-50
references: 21995763
references: Front Plant Sci. 2012 Jul 05;3:149
references: 22783269
references: Commun Integr Biol. 2010 Jan;3(1):28-35
references: 20539778
references: Mol Microbiol. 1997 Jan;23(1):85-93
references: 9004223
references: J Gen Microbiol. 1979 Nov;115(1):167-72
references: 119030
references: Molecules. 2007 Jul 05;12(7):1290-306
references: 17909485
references: Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14587-92
references: 19706545
references: Mol Plant. 2010 May;3(3):491-8
references: 20154222
references: J Biol Chem. 2008 Sep 12;283(37):25247-25255
references: 18635546
references: EMBO J. 1988 Sep;7(9):2621-9
references: 15977331
references: Trends Plant Sci. 1999 Jun;4(6):220-226
references: 10366878
references: Planta. 2005 Nov;222(5):848-57
references: 16025342
references: Plant J. 2008 Feb;53(4):691-704
references: 18269577
references: Plant Physiol. 2008 Nov;148(3):1547-56
references: 18820082
references: Microbes Environ. 2012;27(4):462-9
references: 22972385
references: Appl Environ Microbiol. 2002 Jan;68(1):143-51
references: 11772620
references: Microbiology (Reading). 2008 Jul;154(Pt 7):1845-1858
references: 18599814
references: Bioessays. 1991 Jan;13(1):25-9
references: 1772407
references: Nucleic Acids Res. 2004 Jan 1;32(Database issue):D277-80
references: 14681412
references: PLoS One. 2012;7(4):e35498
references: 22545111
references: Appl Environ Microbiol. 2009 Mar;75(5):1223-8
references: 19114532
references: Appl Environ Microbiol. 2008 Feb;74(3):738-44
references: 18083870