title: Simulated microgravity alters the metastatic potential of a human lung adenocarcinoma cell line.
abstract: Simulated microgravity (SM) has been implicated in affecting diverse cellular pathways. Although there is emerging evidence that SM can alter cellular functions, its effect in cancer metastasis has not been addressed. Here, we demonstrate that SM inhibits migration, gelatinolytic activity, and cell proliferation of an A549 human lung adenocarcinoma cell line in vitro. Expression of antigen MKI67 and matrix metalloproteinase-2 (MMP2) was reduced in A549 cells stimulated by clinorotation when compared with the 1×g control condition, while overexpression of each gene improves ability of proliferation and migration, respectively, under SM conditions. These findings suggest that SM reduced the metastatic potential of human lung adenocarcinoma cells by altering the expression of MKI67 and MMP2, thereby inhibiting cell proliferation, migration, and invasion, which may provide some clues to study cancer metastasis in the future.
authors: Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Beijing, 100853, China.
authors: De
authors: D
authors: Chang
authors: Huiwen
authors: H
authors: Xu
authors: Yinghua
authors: Y
authors: Guo
authors: Xuege
authors: X
authors: Jiang
authors: Yan
authors: Y
authors: Liu
authors: Kailong
authors: K
authors: Li
authors: Chunxiao
authors: C
authors: Pan
authors: Ming
authors: M
authors: Yuan
authors: Junfeng
authors: J
authors: Wang
authors: Tianzhi
authors: T
authors: Li
authors: Changting
authors: C
authors: Liu
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008175:Lung Neoplasms / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009362:Neoplasm Metastasis / Q000473:pathology*; D009687:Nuclear Proteins / Q000378:metabolism; D014893:Weightlessness
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C492406:NIFK protein, human; D009687:Nuclear Proteins; D020778:Matrix Metalloproteinase 2
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