title: Isolation of mesenchymal stem cells from human bone and long-term cultivation under physiologic oxygen conditions.
abstract: Bone-derived stroma cells contain a rare subpopulation, which exhibits enhanced stemness characteristics. Therefore, this particular cell type is often attributed the mesenchymal stem cell (MSC). Due to their high proliferation potential, multipotential differentiation capacity, and immunosuppressive properties, MSCs are now widely appreciated for cell therapeutic applications in a multitude of clinical aspects. In line with this, maintenance of MSC stemness during isolation and culture expansion is considered pivot. Here, we provide step-by-step protocols which allow selection for, and in vitro propagation of high quality MSC from human bone.
authors: The Extracellular Matrix Research Group, Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria.
authors: Sebastian
authors: S
authors: Klepsch
authors: Angelika
authors: A
authors: Jamnig
authors: Daniela
authors: D
authors: Trimmel
authors: Magdalena
authors: M
authors: Schimke
authors: Werner
authors: W
authors: Kapferer
authors: Regina
authors: R
authors: Brunauer
authors: Sarvpreet
authors: S
authors: Singh
authors: Stephan
authors: S
authors: Reitinger
authors: Günter
authors: G
authors: Lepperdinger
mesh_terms: D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002468:Cell Physiological Phenomena / Q000502:physiology*; D049109:Cell Proliferation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D010100:Oxygen