title: A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products.
abstract: Species identification in meat products has grown in interest in recent years since these foodstuffs are susceptible targets for fraudulent labelling. In this work, a real-time PCR approach based on SYBR Green dye was proposed for the quantitative detection of pork meat in processed meat products. For the development of the method, binary meat mixtures containing known amounts of pork meat in poultry meat were used to obtain a normalised calibration model from 0.1 to 25% with high linear correlation and PCR efficiency. The method revealed high specificity by melting curve analysis, being successfully validated through its application to blind meat mixtures, which confirmed its adequacy for pork meat determination. The fully applicability of the method was further demonstrated in commercial meat products, allowing verification of labelling compliance and identification of meat species in processed foods.
authors: Departamento de Ciências Químicas, Universidade do Porto, Portugal.
authors: Sónia
authors: S
authors: Soares
authors: Joana S
authors: JS
authors: Amaral
authors: M Beatriz P P
authors: MB
authors: Oliveira
authors: Isabel
authors: I
authors: Mafra
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D008192:Deception; D003959:Diamines; D004032:Diet; D005456:Fluorescent Dyes; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D005515:Food Labeling; D006801:Humans; D008460:Meat; D008461:Meat Products / Q000032:analysis*; D009930:Organic Chemicals; D011200:Poultry / Q000235:genetics*; D011804:Quinolines; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013552:Swine / Q000235:genetics*
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I