title: Urea's effect on the ribonuclease A catalytic efficiency: a kinetic, 1H NMR and molecular orbital study.
abstract: Understanding of protein-urea interactions is one of the greatest challenges to modern structural protein chemistry. Based in enzyme kinetics experiments and (1)H NMR spectroscopic analysis we proposed that urea, at low concentrations, directly interacts with the protonated histidines of the active center of RNase A, following a simple model of competitive inhibition. These results were supported by theoretical analysis based on the frontier molecular orbital theory and suggest that urea might establish a favorable interaction with the cationic amino acids. Our experimental evidence and theoretical analysis indicate that the initials steps of the molecular mechanism of Urea-RNase A interaction passes through the establishment of a three center four electron adduct. Also, our results would explain the observed disruption of the (1)H NMR signals corresponding to H12 and H119 (involved in catalysis) of the RNase A studied in the presence of urea. Our interaction model of urea-amino acids (cationic) can be extended to explain the inactivation of other enzymes with cationic amino acids at the active site.
authors: Laboratorio de Genética y Química Celular, Departamento de Biología, Facultad de Ciencias, Universidad de Los Andes, Mérida, Venezuela.
authors: Jorge
authors: J
authors: Almarza
authors: Luis
authors: L
authors: Rincón
authors: Alí
authors: A
authors: Bahsas
authors: María Angela
authors: MA
authors: Pinto
authors: Francisco
authors: F
authors: Brito
mesh_terms: D000818:Animals; D055162:Biocatalysis; D002417:Cattle; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D011489:Protein Denaturation; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D014508:Urea / Q000737:chemistry*
chemical_list: D014508:Urea; D012259:Ribonuclease, Pancreatic
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