title: Characterization of a heteropolysaccharide isolated from diploid Gynostemma pentaphyllum Makino.
abstract: A novel water-soluble polysaccharide (GPP), with a molecular mass of 7.1×10(3) Da, was isolated from the defatted whole-plant of diploid Gynostemma pentaphyllum Makino. Monosaccharide composition analysis indicated that GPP was a heteropolysaccharide mainly containing mannose, glucose, galactose and arabinose, at a molar ratio of 1.00:77.33:4.81:1.83. The detailed structure analysis revealed that GPP consisted of a (1→4)-α-D-glucoside backbone with a 1→)-α-D-glucopyranosyl branch at the C-6 position of (1→4,6)-linked-α-D-glucopyranosyl on every 5 monosaccharide residues, with a few mannose, galactose and arabinose terminal residues. GPP exhibited scavenging capacities against hydroxyl, peroxyl and DPPH radicals in vitro, and had a greater bile acid-binding ability than psyllium on a per weight basis. These results suggested a potential application of GPP in functional foods and dietary supplements.
authors: State Key Lab of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
authors: Wei
authors: W
authors: Yan
authors: Yuge
authors: Y
authors: Niu
authors: Junli
authors: J
authors: Lv
authors: Zhuohong
authors: Z
authors: Xie
authors: Lei
authors: L
authors: Jin
authors: Wenbing
authors: W
authors: Yao
authors: Xiangdong
authors: X
authors: Gao
authors: Liangli Lucy
authors: LL
authors: Yu
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D004171:Diploidy; D029774:Gynostemma / Q000737:chemistry* / Q000235:genetics*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001647:Bile Acids and Salts; D009005:Monosaccharides; D011134:Polysaccharides