title: [Expression of CD80 and CD86 on dendritic cells of patients with immune related pancytopenia and its clinical significance].
abstract: OBJECTIVE
  To investigate the function of dendritic cells (DC) of patients with immune related pancytopenia (IRP) and explore the role of DC in IRP.
  
  
  METHODS
  The expression of CD80 and CD86 on myeloid DC (mDC, Lin-HLA-DR(+) CD11c(+) cells) and plasmacytoid DC (pDC, Lin-HLA-DR(+) CD123(+) cells) of 65 IRP (37 untreated and 28 remitted) patients and 17 healthy controls were analyzed by flow cytometry.
  
  
  RESULTS
  The expression of CD86 on pDC was (82.47 ± 13.17)% in untreated group and (60.08 ± 14.29)% in remission group, which were significantly higher than that of controls (47.95 ± 18.59)% (P < 0.05), while the expression in untreated group was higher than that of remission group (P < 0.05). The expression of CD80 on pDC was (6.31 ± 4.49)% in untreated group, which was significantly higher than that of remitted patients (3.09 ± 2.93)% and controls (2.33 ± 2.25)% (P < 0.05). The expression of CD86 on mDC was (97.06 ± 4.82)% in untreated group and (91.35 ± 12.20)% in control group, while the expression in untreated group was higher than that of control group (P < 0.05). The expression of CD80 on mDC was (6.20 ± 5.44)% in untreated group and (3.97 ± 3.24)% in remission group, which were significantly higher than that of controls (1.86 ± 1.73)% (P < 0.05). The expression of CD86 on pDC was negatively correlated to Th1/Th2 (r = -0.733, P < 0.05), it was positively correlated to the antibody on membrane of BMMNC (r = 0.283, P < 0.05) and the quantity of CD5(+)B cells (r = 0.436, P < 0.05), while it was negatively correlated to the level of hemoglobin, platelets and white blood cells (r = -0.539, P < 0.05; r = -0.519, P < 0.05; r = -0.567, P < 0.05, respectively). The expression of CD80 on pDC was negatively correlated to the level of hemoglobin and platelets (r = -0.431, P < 0.05; r = -0.464, P < 0.05).
  
  
  CONCLUSIONS
  The function of pDC in PB of IRP were strengthened, which was relevant to the immunopathogenesis of IRP.
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin, China.
authors: Guang-shuai
authors: GS
authors: Teng
authors: Rong
authors: R
authors: Fu
authors: Hui
authors: H
authors: Liu
authors: Hong-lei
authors: HL
authors: Wang
authors: Yi-hao
authors: YH
authors: Wang
authors: Er-bao
authors: EB
authors: Ruan
authors: Wen
authors: W
authors: Qu
authors: Yong
authors: Y
authors: Liang
authors: Guo-jin
authors: GJ
authors: Wang
authors: Xiao-ming
authors: XM
authors: Wang
authors: Hong
authors: H
authors: Liu
authors: Yu-hong
authors: YH
authors: Wu
authors: Jia
authors: J
authors: Song
authors: Hua-quan
authors: HQ
authors: Wang
authors: Li-min
authors: LM
authors: Xing
authors: Jing
authors: J
authors: Guan
authors: Jun
authors: J
authors: Wang
authors: Li-juan
authors: LJ
authors: Li
authors: Zong-hong
authors: ZH
authors: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D001327:Autoimmune Diseases / Q000150:complications; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010198:Pancytopenia / Q000097:blood* / Q000209:etiology* / Q000473:pathology; D055815:Young Adult
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen