title: Euryhalocaulis caribicus gen. nov., sp. nov., a new member [corrected] of the family Hyphomonadaceae isolated from the Caribbean Sea.
abstract: A new aerobic, Gram-negative, chemo-organotrophic, euryhaline bacterium, designated strain JL2009(T), was isolated from surface water of the Caribbean Sea. The strain formed flaxen colonies on marine ager 2216 (MA; Difco) medium. Cells were dimorphic, with stalks or a polar flagellum, and reproduction occurred by means of binary fission. Growth occurred at 15-45 °C (optimum at 35 °C), 0-15 % (w/v) NaCl (optimum at 1-10 %) and pH 5-9 (optimum at pH 7-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct evolutionary lineage within the family Hyphomonadaceae. Strain JL2009(T) was most closely related to Maricaulis parjimensis MCS 25(T) (92.2 % DNA sequence similarity), Woodsholea maritime CM2243(T) (90.9 %), and Oceanicaulis alexandrii C116-18(T) (90.9 %). The major respiratory quinone was Q-10. The dominant cellular fatty acids were summed feature 8 (C(18:1) ω7c), C(18:0) and 11-methyl C(18:1) ω7c. The polar lipid pattern indicated the presence of phospholipid, phosphatidyl glycerol and glycolipids. The G + C content of the genomic DNA was 70.5 mol%. On the basis of the chemotaxonomic and phenotypic characteristics and the phylogenetic evidence, strain JL2009(T) represents a novel genus and species in the family Hyphomonadaceae, for which the name Euryhalocaulis caribicus gen. nov., sp. nov. is proposed. The type strain of Euryhalocaulis caribicus is JL2009(T) (=CGMCC 1.12036(T) = JCM 18163(T)).
authors: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, People's Republic of China. dengwenchao@stu.xmu.edu.cn
authors: Wenchao
authors: W
authors: Deng
authors: Yao
authors: Y
authors: Zhang
authors: Xiabing
authors: X
authors: Xie
authors: Zihao
authors: Z
authors: Zhao
authors: Yingnan
authors: Y
authors: Fu
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D017691:Caribbean Region; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
references: Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):716-721
references: 22140171
references: J Microbiol. 2009 Apr;47(2):156-61
references: 19412598
references: Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):722-727
references: 20418412
references: Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1534-1538
references: 20656814
references: J Bacteriol. 1991 Jan;173(2):697-703
references: 1987160
references: Int J Syst Bacteriol. 1990 Oct;40(4):443-51
references: 2275859
references: Appl Environ Microbiol. 1998 Mar;64(3):992-8
references: 16349532
references: Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):314-320
references: 22427444
references: Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2511-9
references: 18984685
references: J Chromatogr. 1989 Oct 6;479(2):297-306
references: 2509507
references: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references: 9396791
references: Can J Biochem Physiol. 1959 Aug;37(8):911-7
references: 13671378
references: Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):2951-5
references: 19628598
references: Int J Syst Evol Microbiol. 2003 Nov;53(Pt 6):1901-6
references: 14657121
references: Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1227-1234
references: 15280296
references: Am J Clin Pathol. 1966 Apr;45(4):493-6
references: 5325707
references: Int J Syst Evol Microbiol. 2002 Nov;52(Pt 6):2191-2201
references: 12508888
references: Bacteriol Rev. 1964 Sep;28:231-95
references: 14220656
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2595-2599
references: 17978224
references: Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):1907-1919
references: 16166687
references: Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3083-7
references: 19643895