title: Structural elucidation of a novel heteropolysaccharide from the fruiting bodies of Pleurotus eryngii.
abstract: A novel water-soluble heteropolysaccharide (PEPS1), with a molecular weight of 1.88×10(4) Da as determined by high performance liquid chromatography (HPLC), specifically size exclusion chromatography, was isolated from the fruiting bodies of Pleurotus eryngii by hot water extraction and further purification by DEAE Sepharose Fast Flow and Sephacryl S-300 and S-100 High-Resolution chromatography. By use of compositional analysis, methylation analysis, together with (1)H, (13)C NMR and 2D NMR spectroscopy including COSY, TOCSY, HMQC, HMBC and NOESY experiments, the PEPS1 was identified as a heteropolysaccharide that consists of a α-D-(1→6)-Galactopyranan backbone with a β-D-Mannosyl unit on O-2 of the 2,6-di-O-substituted-D-Galactosyl units, α-(1→6)-3-O-Me-D-Galactopyranan backbone with a terminal α-3-O-Me-D-Galactosyl unit and it also contained a minor 2,3,6-α-D-Galatopyranan units and 4-β-D-Mannopyranan residues.
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors: An-qiang
authors: AQ
authors: Zhang
authors: Yang
authors: Y
authors: Zhang
authors: Jun-hong
authors: JH
authors: Yang
authors: Pei-long
authors: PL
authors: Sun
mesh_terms: D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D008745:Methylation; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D011134:Polysaccharides