title: In vivo multimodal microscopy for detecting bone-marrow-derived cell contribution to skin regeneration.
abstract: Bone-marrow (BM)-derived cells have been shown to be capable of aiding skin regeneration in vivo by differentiating into keratinocytes. However, the conditions under which this occurs are not fully understood. Characterizing innate mechanisms of skin regeneration by stem cells in vivo is important for the area of stem cell biology. In this study, we investigate the use of novel in vivo imaging technology for characterizing the contribution of BM-derived cells to regeneration of the epidermis in mouse skin in vivo. In vivo imaging provides the ability to non-invasively observe the spatial positions and morphology of the BM-derived cells. Using a GFP BM-transplanted mouse model and in vivo multimodal microscopy, BM-derived cells can be observed in the skin. Our in vivo imaging method was used to search for the presence and identify the 3D spatial distribution of BM-derived cells in the epidermis of the skin under normal conditions, following wound healing, and after syngeneic skin grafting. We did not observe any evidence of BM-derived keratinocytes under these conditions, but we did observe BM-derived dendritic cells in the skin grafts. In vivo multimodal imaging has great potential for characterizing the conditions under which BM-derived cells contribute to skin regeneration.
authors: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL.
authors: Benedikt W
authors: BW
authors: Graf
authors: Andrew J
authors: AJ
authors: Bower
authors: Eric J
authors: EJ
authors: Chaney
authors: Marina
authors: M
authors: Marjanovic
authors: Steven G
authors: SG
authors: Adie
authors: Michael
authors: M
authors: De Lisio
authors: Maria C
authors: MC
authors: Valero
authors: Marni D
authors: MD
authors: Boppart
authors: Stephen A
authors: SA
authors: Boppart
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008853:Microscopy / Q000379:methods*; D064847:Multimodal Imaging; D061848:Optical Imaging; D012038:Regeneration; D012867:Skin / Q000166:cytology*; D012879:Skin Physiological Phenomena; D016038:Skin Transplantation
keywords: bone marrow cells; in vivo microscopy; multimodal; skin regeneration
references: J Dermatol Sci. 2005 Nov;40(2):85-94
references: 16139481
references: Histochem J. 2000 Feb;32(2):99-103
references: 10816074
references: Wound Repair Regen. 2007 Sep-Oct;15 Suppl 1:S18-26
references: 17727462
references: Nat Biotechnol. 2003 Nov;21(11):1356-60
references: 14595363
references: Opt Express. 2006 Aug 21;14(17):7821-40
references: 19529151
references: Opt Lett. 2000 Jan 15;25(2):114-6
references: 18059800
references: Stem Cells. 2010 May;28(5):905-15
references: 20474078
references: Proc Natl Acad Sci U S A. 2011 Apr 19;108(16):6609-14
references: 21464317
references: Science. 1991 Nov 22;254(5035):1178-81
references: 1957169
references: J Cell Physiol. 2003 Aug;196(2):245-50
references: 12811816
references: Tissue Eng. 2005 Jan-Feb;11(1-2):110-9
references: 15738666
references: Science. 1990 Apr 6;248(4951):73-6
references: 2321027
references: Stem Cells. 2004;22(5):812-22
references: 15342945
references: J Biomed Opt. 2009 May-Jun;14(3):034019
references: 19566312
references: Nat Rev Immunol. 2008 Dec;8(12):935-47
references: 19029989
references: Exp Hematol. 2006 May;34(5):672-9
references: 16647573
references: Nat Immunol. 2002 Dec;3(12):1135-41
references: 12415265
references: Nat Immunol. 2006 Mar;7(3):265-73
references: 16444257
references: J Pathol. 2005 Jan;205(1):1-13
references: 15546160
references: FEBS Lett. 1997 May 5;407(3):313-9
references: 9175875
references: Am J Pathol. 2004 Nov;165(5):1767-72
references: 15509544