title: Spatiotemporal expression of SKIP after rat sciatic nerve crush.
abstract: Ski-interacting protein (SKIP) is a highly conserved protein from yeast to Human. As an essential spliceosomal component and transcriptional co-regulator it plays an important role in preinitiation, splicing and polyadenylation. SKIP can also combine with Ski to overcome the G1 arrest and the growth-suppressive activities of pRb. Furthermore SKIP has the capacity to augment TGF-β dependent transcription. While the distribution and function of SKIP in peripheral nervous system lesion and regeneration remain unclear. Here, we investigated the spatiotemporal expression of SKIP in an acute sciatic nerve crush model in adult rats. Western Blot analysis revealed that SKIP was expressed in normal sciatic nerves. It gradually increased, reached a peak at 1 week after crush, and then returned to the normal level at 4 weeks. Besides, we observed that up-regulation of SKIP was approximately in parallel with Proliferating cell nuclear antigen (PCNA), and numerous Schwann cells (SCs) expressing SKIP were PCNA and Ki-67 positive. Collectively, we hypothesized peripheral nerve crush induced up-regulation of SKIP in the sciatic nerve, which was associated with SCs proliferation.
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, 20 Xi-Si Road, Nantong, 226001 Jiangsu, People's Republic of China. wangyouhuapro@yahoo.com.cn
authors: Youhua
authors: Y
authors: Wang
authors: Long
authors: L
authors: Long
authors: Jiao
authors: J
authors: Yang
authors: Yajuan
authors: Y
authors: Wu
authors: Hao
authors: H
authors: Wu
authors: Haixiang
authors: H
authors: Wei
authors: Xiaolong
authors: X
authors: Deng
authors: Xinghai
authors: X
authors: Cheng
authors: Dong
authors: D
authors: Lou
authors: Hailei
authors: H
authors: Chen
authors: Hai
authors: H
authors: Wen
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D008297:Male; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; C581694:SKIP protein, rat; D014157:Transcription Factors
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