title: Inhibitory effects of blockage of intermediate conductance Ca(2+)-activated K          (+) channels on proliferation of hepatocellular carcinoma cells.
abstract: The roles of intermediate conductance Ca(2+)-activated K(+) channel (IKCa1) in the pathogenesis of hepatocellular carcinoma (HCC) were investigated. Immunohistochemistry and Western blotting were used to detect the expression of IKCa1 protein in 50 HCC and 20 para-carcinoma tissue samples. Real-time PCR was used to detect the transcription level of IKCa1 mRNA in 13 HCC and 11 para-carcinoma tissue samples. The MTT assay was used to measure the function of IKCa1 in human HCC cell line HepG2 in vitro. TRAM-34, a specific blocker of IKCa1, was used to intervene with the function of IKCa1. As compared with para-carcinoma tissue, an over-expression of IKCa1 protein was detected in HCC tissue samples (P<0.05). The mRNA expression level of IKCa1 in HCC tissues was 2.17 times higher than that in para-carcinoma tissues. The proliferation of HepG2 cells was suppressed by TRAM-34 (0.5, 1.0, 2.0 and 4.0 μmol/L) in vitro (P<0.05). Our results suggested that IKCa1 may play a role in the proliferation of human HCC, and IKCa1 blockers may represent a potential therapeutic strategy for HCC.
authors: Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Xiao-Wei
authors: XW
authors: Yang
authors: Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. liujinwen@tjh.tjmu.edu.cn.
authors: Jin-Wen
authors: JW
authors: Liu
authors: Department of General Surgery, Yichang Central People's Hospital, the First College of Clinical Medical Science, China Three Gorges University, Yichang, 443003, China.
authors: Ru-Chao
authors: RC
authors: Zhang
authors: Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Qian
authors: Q
authors: Yin
authors: Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Wen-Zhuang
authors: WZ
authors: Shen
authors: Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Ji-Lin
authors: JL
authors: Yi
mesh_terms: D002121:Calcium Channel Blockers / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000503:physiopathology*; D049109:Cell Proliferation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015640:Ion Channel Gating / Q000187:drug effects; D008113:Liver Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D011188:Potassium / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D002121:Calcium Channel Blockers; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C496923:KCNN4 protein, human; D011720:Pyrazoles; C411671:TRAM 34; D011188:Potassium
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