title: Evaluation of PNA-FISH yeast traffic light for rapid identification of yeast directly from positive blood cultures and assessment of clinical impact.
abstract: The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 94.8%; C. tropicalis, 100%). Case note review estimated a change in therapy in 29% of cases had the PNA-FISH result been available to the clinician.
authors: Department of Microbiology, Royal Free Hospital, London, United Kingdom. neil.stone@nhs.net
authors: N R H
authors: NR
authors: Stone
authors: R L
authors: RL
authors: Gorton
authors: K
authors: K
authors: Barker
authors: P
authors: P
authors: Ramnarain
authors: C C
authors: CC
authors: Kibbler
mesh_terms: D002175:Candida / Q000235:genetics / Q000302:isolation & purification*; D058387:Candidemia / Q000175:diagnosis* / Q000382:microbiology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009172:Mycology / Q000379:methods*; D012680:Sensitivity and Specificity
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