title: Effects of high temperature and exposure to air on mussel (Mytilus galloprovincialis, Lmk 1819) hemocyte phagocytosis: modulation of spreading and oxidative response.
abstract: Hemocytes are a critical component of the mussel defense system and the present study aims at investigating their spreading and oxidative properties during phagocytosis under in vivo experimental stress conditions. The spreading ability was measured by an automated cell analyzer on the basis of the circularity, a parameter corresponding to the hemocyte roundness. The oxidative activity was investigated by micromethod assay, measuring the respiratory burst as expression of the fluorescence generated by the oxidation of specific probe. Following the application of high temperature and exposure to air, there was evidence of negative modulation of spreading and oxidative response, as revealed by a cell roundness increase and fluorescence generation decrease. Therefore, the fall of respiratory burst appeared as matched with the inhibition of hemocyte morphological activation, suggesting a potential depression of the phagocytosis process and confirming the application of the circularity parameter as potential stress marker, both in experimental and field studies.
authors: Dipartimento di Scienze Biomediche Comparate, Università degli Studi di Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy.
authors: Francesco
authors: F
authors: Mosca
authors: Valeria
authors: V
authors: Narcisi
authors: Angela
authors: A
authors: Calzetta
authors: Luisa
authors: L
authors: Gioia
authors: Maria G
authors: MG
authors: Finoia
authors: Mario
authors: M
authors: Latini
authors: Pietro G
authors: PG
authors: Tiscar
mesh_terms: D000388:Air; D000818:Animals; D049872:Bivalvia / Q000166:cytology* / Q000254:growth & development; D005296:Ferrous Compounds / Q000494:pharmacology; D005453:Fluorescence; D006434:Hemocytes / Q000166:cytology*; D006358:Hot Temperature; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010587:Phagocytosis / Q000187:drug effects*; D016897:Respiratory Burst / Q000187:drug effects
chemical_list: D005296:Ferrous Compounds; C031621:ferrous fumarate