title: Tumor-specific RNA interference targeting Pokemon suppresses tumor growth and induces apoptosis in prostate cancer.
abstract: OBJECTIVE
  To explore the exact mechanism of Pokemon in prostate cancer.
  
  
  METHODS
  Pokemon is a member of the POK family of transcriptional repressors. Its main function is suppression of the p14ARF (alternate reading frame) tumor suppressor gene. Although Pokemon expression has been found to be increased in various types of lymphoma, the exact mechanism of the gene in prostate cancer is not clear. In the present study, prostate cancer cells were transfected with the specific short hairpin ribonucleic acid (RNA) expression vector targeting Pokemon. The expression of Pokemon messenger RNA and its protein was detected by semiquantitative reverse transcriptase-polymerase chain reaction and Western blotting, respectively. The cell growth and cell apoptosis were also examined using the methyl thiazolyl tetrazolium assay and flow cytometry.
  
  
  RESULTS
  The results demonstrated that specific RNA interference (RNAi) could decrease the expression levels of Pokemon gene messenger RNA and protein in prostate cancer cells. In addition, that specific RNAi significantly inhibited the cell proliferation and increased the apoptotic rate. In vivo experiments showed that specific RNAi inhibited the tumorigenicity of prostate cancer cells and significantly suppressed tumor growth.
  
  
  CONCLUSIONS
  Therefore, an RNAi-targeted Pokemon gene strategy could be a potential approach to prostate cancer therapy.
authors: Department of Urology, Second Affiliated Hospital, Fujian Medical University, Quanzhou Fujian, China.
authors: Yining
authors: Y
authors: Li
authors: Shuxiong
authors: S
authors: Xu
authors: Xiangwei
authors: X
authors: Wang
authors: Hua
authors: H
authors: Shi
authors: Zhaolin
authors: Z
authors: Sun
authors: Zhao
authors: Z
authors: Yang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000256:Adenoviridae; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014157:Transcription Factors; C485514:ZBTB7A protein, human; D053148:Caspase 3