title: Assessment of CD4(+) and CD8 (+) T cell responses using MHC class I and II tetramers.
abstract: The low frequency of T cells specific for given antigens makes the study of antigen-specific T cell responses difficult. The development of MHC class I and II tetramer staining techniques allows precise quantification and tracking of antigen-specific CD8(+) and CD4(+) T cell responses. Here, we describe a protocol for MHC class I and II tetramer staining of mouse T cells isolated from various tissues of mice infected with lymphocytic choriomeningitis virus (LCMV) or with murine cytomegalovirus (MCMV).
authors: Division of Immunobiology, Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA.
authors: Sema
authors: S
authors: Kurtulus
authors: David
authors: D
authors: Hildeman
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D002469:Cell Separation; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000737:chemistry* / Q000378:metabolism; D008217:Lymphocytic choriomeningitis virus / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018146:Muromegalovirus / Q000502:physiology; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling; D014709:Vero Cells
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II
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