title: Identification of fatty acids in canine seminal plasma.
abstract: Seminal plasma contains various biochemical components associated with sperm function. However, there is limited information regarding the fatty acid composition of seminal plasma and their effect on sperm. The aim of this study was to identify the fatty acid content in canine seminal plasma using gas chromatography. Twelve ejaculates were studied, the seminal plasma was obtained by centrifugation and then the lipids were extracted, methylated and analysed by chromatography. The total lipids in the seminal plasma were 2.5 ± 0.3%, corresponding to 85% saturated fatty acids (SFA) and 15% unsaturated fatty acids (UFA). The greatest proportions of SFA were palmitic acid (30.4%), stearic acid (23.4%) and myristic acid (5.3%) and of UFA oleic acid (9.0%). Therefore, the protocols and techniques used enabled the identification of 18 different fatty acids in canine seminal plasma, which constitutes a good method to evaluate and quantify the fatty acid profile in this species.
authors: Laboratorio de Producción Animal, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Temuco, Chile; Programa de Doctorado en Ciencias mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, Temuco, Chile.
authors: R
authors: R
authors: Díaz
authors: K
authors: K
authors: Inostroza
authors: J
authors: J
authors: Risopatrón
authors: R
authors: R
authors: Sanchez
authors: N
authors: N
authors: Sepúlveda
mesh_terms: D000818:Animals; D004285:Dogs; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008297:Male; D019814:Myristic Acid / Q000032:analysis; D019301:Oleic Acid / Q000032:analysis; D019308:Palmitic Acid / Q000032:analysis; D012661:Semen / Q000737:chemistry*; D013229:Stearic Acids / Q000032:analysis
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D013229:Stearic Acids; D019814:Myristic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid
keywords: Canine semen; Canis familiaris domesticus; gas chromatography