title: Olfactory ensheathing cells form the microenvironment of migrating GnRH-1 neurons during mouse development.
abstract: During development, GnRH-1 neurons differentiate extracerebraly from the nasal placode and migrate from the vomeronasal organ to the forebrain along vomeronasal and terminal nerves. Numerous studies have described the influence of different molecules on the migration of GnRH-1 neurons, however, the role of microenvironment cells remains poorly understood. This study used GFAP-GFP transgenic mice to detect glial cells at early developmental stages. Using nasal explant cultures, the comigration of glial cells with GnRH-1 neurons was clearly demonstrated. This in vitro approach showed that glial cells began migrating from the explants before GnRH-1 neurons. They remained ahead of the GnRH-1 migratory front and stopped migrating after the GnRH-1 neurons. The association of these glial cells with the axons combined with gene expression analysis of GFAP-GFP sorted cells enabled them to be identified as olfactory ensheathing cells (OEC). Immunohistochemical analysis revealed the presence of multiple glial cell-type markers showing several OEC subpopulations surrounding GnRH-1 neurons. Moreover, these OEC expressed genes whose products are involved in the migration of GnRH-1 neurons, such as Nelf and Semaphorin 4. In situ data confirmed that the majority of the GnRH-1 neurons were associated with glial cells along the vomeronasal axons in nasal septum and terminal nerves in the nasal forebrain junction as early as E12.5. Overall, these data demonstrate an OEC microenvironment for migrating GnRH-1 neurons during mouse development. The fact that this glial cell type precedes GnRH-1 neurons and encodes for molecules involved in their nasal migration suggests that it participates in the GnRH-1 system ontogenesis.
authors: Physiologie de la Reproduction et des Comportements, UMR 0085 INRA, 6175 CNRS, Université François Rabelais de Tours, IFCE, IFR135 Imagerie Fonctionnelle 37380, Nouzilly, France.
authors: Sarah
authors: S
authors: Geller
authors: Elise
authors: E
authors: Kolasa
authors: Yves
authors: Y
authors: Tillet
authors: Anne
authors: A
authors: Duittoz
authors: Pascal
authors: P
authors: Vaudin
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D060833:Cellular Microenvironment / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein; D007987:Gonadotropin-Releasing Hormone / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics; D009830:Olfactory Bulb / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D009831:Olfactory Mucosa / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D009924:Organ Culture Techniques; D011401:Promoter Regions, Genetic / Q000235:genetics; D011498:Protein Precursors / Q000502:physiology*; D011817:Rabbits
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; C528900:glial fibrillary astrocytic protein, mouse; C050866:progonadoliberin I; D007987:Gonadotropin-Releasing Hormone