title: Realgar bioleaching solution is a less toxic arsenic agent in suppressing the Ras/MAPK pathway in Caenorhabditis elegans.
abstract: To explore other arsenic derivatives with anticancer effects and fewer adverse effects, realgar bioleaching solution (RBS) has been found to be a viable approach. Here we used C. elegans as a model organism to its possible efficacy for anti-cancer effect of RBS. Our results indicated that RBS significantly suppressed the multivulva (Muv) phenotype of let-60 ras(gf) mutant that was positive correlated to arsenic concentrations in worms and also inhibited Muv phenotype of lin-15(lf) upstream of Ras/MAPK pathway, but did not affect the Muv phenotype resulting from loss-of-function mutations of lin-l(lf) downstream of Ras/MAPK pathway, which may be mechanism-based. In toxicity tests, RBS did not lead to reduction resulting from arsenic trioxide (ATO) in the number of pharyngeal pumping which was orthologous to vertebrate heart beating in wild type C. elegans. Overall, RBS was likely to be a potential anti-cancer drug candidate with high efficiency and low toxicity.
authors: MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life sciences, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors: Dongling
authors: D
authors: Liu
authors: Dejuan
authors: D
authors: Zhi
authors: Ting
authors: T
authors: Zhou
authors: Qinghong
authors: Q
authors: Yu
authors: Fengqi
authors: F
authors: Wan
authors: Yanli
authors: Y
authors: Bai
authors: Hongyu
authors: H
authors: Li
mesh_terms: D000818:Animals; D001152:Arsenicals; D049628:Body Size / Q000187:drug effects; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000187:drug effects; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D009154:Mutation; D010614:Pharynx / Q000187:drug effects / Q000502:physiology; D010641:Phenotype; D012996:Solutions; D013440:Sulfides / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001152:Arsenicals; D029742:Caenorhabditis elegans Proteins; D012996:Solutions; D013440:Sulfides; D014157:Transcription Factors; C088818:lin-15A protein, C elegans; C066646:let-60 protein, C elegans; C058317:arsenic disulfide; D018631:ras Proteins