title: Colorimetric and luminescent dual-signaling responsive probing of thiols by a ruthenium(II)-azo complex.
abstract: A dinuclear ruthenium(II) complex linked via a reducible azo group [Ru(bpy)2(azobpy)Ru(bpy)2]Cl4 (Ru2azo, bpy=2,2'-bipyridine, azobpy=4,4″-azobis (2,2'-bipyridine)) was adopted as a probe for thiols. Results showed that Ru2azo could selectively and effectively react with biological thiols (such as cysteine, homocysteine and glutathione) with a 10(-7)M detection limit. After it reacted with thiols, the original gray color of Ru2azo solution immediately turned yellow and the luminescence significantly enhanced, showing "naked-eye" colorimetric and "off-on" luminescent dual-signaling response for thiols. Mechanism studies demonstrated that Ru2azo reacted with thiols undergoing a two-electron transfer process, forming the azo(2-) anion product.
authors: MOE Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors: Guan-Ying
authors: GY
authors: Li
authors: Jiang-Ping
authors: JP
authors: Liu
authors: Huai-Yi
authors: HY
authors: Huang
authors: Ya
authors: Y
authors: Wen
authors: Hui
authors: H
authors: Chao
authors: Liang-Nian
authors: LN
authors: Ji
mesh_terms: D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis*; D004579:Electron Transport; D005978:Glutathione / Q000032:analysis*; D006710:Homocysteine / Q000032:analysis*; D057230:Limit of Detection; D008163:Luminescent Measurements; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D012996:Solutions
chemical_list: D056831:Coordination Complexes; D015335:Molecular Probes; D012996:Solutions; D006710:Homocysteine; D012428:Ruthenium; D005978:Glutathione; D003545:Cysteine