title: Cytotoxic activity of Guettarda pohliana Müll. Arg. (Rubiaceae).
abstract: The cytotoxic activity of crude extracts and their fractions from leaves and roots of G. pohliana was assessed against nine human cancer cell lines: melanoma (UACC-62), breast (MCF-7), breast expressing the multidrug resistance phenotype (NCI-ADR), lung (NCI-460), prostate (PCO-3), kidney (786-0), ovarian (OVCAR), colon (HT-29) and leukaemia (K-562). The hexane fraction from leaves (HL) and ethyl acetate (EAR), chloroform (CR) and hydromethanolic (HMR) fractions from roots were the most active fractions against K-562 with GI₅₀ values being lower than 1 μg mL⁻¹. Also, CR and HMR fractions were active against UACC-62 cell line in the same order of magnitude. The phytochemical study of the CR fraction allowed identifying the known iridoids secoxyloganin, sweroside and loganin.
authors: a Departamento de Química , Universidade Estadual de Maringá , Av. Colombo 5790 , 87020-900 , Maringá , Paraná , Brazil.
authors: Paulo R N
authors: PR
authors: de Oliveira
authors: Gláucio
authors: G
authors: Testa
authors: Rebeca P
authors: RP
authors: Medina
authors: Cecília M A
authors: CM
authors: de Oliveira
authors: Lucilia
authors: L
authors: Kato
authors: Cleuza C
authors: CC
authors: da Silva
authors: João E
authors: JE
authors: de Carvalho
authors: Silvana M O
authors: SM
authors: Santin
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019073:HT29 Cells; D006801:Humans; D057888:Iridoid Glucosides / Q000737:chemistry / Q000494:pharmacology; D039823:Iridoids / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; C059515:secoxyloganin; C059516:loganin