title: A differentially displayed mRNA related to resistance to Bacillus thuringiensis israelensis of Aedes albopictus selected in vitro-activated Cyt1Aa6.
abstract: We compared the differential display of Aedes albopictus cells, both resistant and susceptible to Bacillus thuringiensis israelensis (Bti), using differentially displayed reverse transcription polymerase chain reaction. We found 1 band about 200 base pairs long. After cloning and sequencing, the differentially expressed gene was similar to some partial messenger ribonucleic acid of Ae. aegypti, Culex quinquefasciatus, and Anopheles gambiae, rather than Ae. albopictus. This will be of some value for clarifying the mechanism of mosquito resistance to Bti products.
authors: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, People's Republic of China.
authors: Lingling
authors: L
authors: Zhang
authors: Qi
authors: Q
authors: Zhang
authors: Enjiong
authors: E
authors: Huang
authors: Mingwei
authors: M
authors: Li
authors: Tianpei
authors: T
authors: Huang
authors: Lei
authors: L
authors: Xu
authors: Chanbiao
authors: C
authors: Wu
authors: Xiong
authors: X
authors: Guan
authors: Ivan
authors: I
authors: Gelbic
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D004731:Endotoxins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000502:physiology; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007305:Insecticide Resistance / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D012333:RNA, Messenger; C030956:insecticidal crystal protein, Bacillus Thuringiensis