title: Physical mapping and cloning of RAD56.
abstract: Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.
authors: Department of Biology, University of Copenhagen, Ole Maaloeesvej 5, DK-2200 Copenhagen N, Denmark.
authors: David P
authors: DP
authors: Mathiasen
authors: Irene
authors: I
authors: Gallina
authors: Susanne M
authors: SM
authors: Germann
authors: Wissam
authors: W
authors: Hamou
authors: Morgane
authors: M
authors: Eléouët
authors: Sara
authors: S
authors: Thodberg
authors: Nadine
authors: N
authors: Eckert-Boulet
authors: John
authors: J
authors: Game
authors: Michael
authors: M
authors: Lisby
mesh_terms: D000107:Acetylation; D001761:Bleomycin / Q000009:adverse effects; D002166:Camptothecin / Q000009:adverse effects; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics; D006918:Hydroxyurea / Q000009:adverse effects; D008741:Methyl Methanesulfonate / Q000009:adverse effects; D009154:Mutation; D063210:N-Terminal Acetyltransferase B / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014965:X-Rays / Q000009:adverse effects
chemical_list: D004271:DNA, Fungal; C570986:Nat3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D001761:Bleomycin; C105427:Zeocin; D008741:Methyl Methanesulfonate; D063210:N-Terminal Acetyltransferase B; D006918:Hydroxyurea; D002166:Camptothecin