title: Time-of-flight magnetic flow cytometry in whole blood with integrated sample preparation.
abstract: Rapid and specific rare cell detection for point-of-care testing requires an integration of the sample preparation for flow cytometry. To achieve such a challenging goal we have developed a magnetic flow cytometry technique which applies magnetophoresis to perform cell enrichment, focusing, and background elimination in a single step. Time-of-flight measurements are performed with integrated magnetic sensors to detect specifically cancer cells and cell diameters in whole blood.
authors: Siemens AG, Corporate Technology, Günther-Scharowsky Strasse 1, 91058 Erlangen, Germany. michaeljohannes.helou.ext@siemens.com
authors: Michael
authors: M
authors: Helou
authors: Mathias
authors: M
authors: Reisbeck
authors: Sandro F
authors: SF
authors: Tedde
authors: Lukas
authors: L
authors: Richter
authors: Ludwig
authors: L
authors: Bär
authors: Jacobus J
authors: JJ
authors: Bosch
authors: Roland H
authors: RH
authors: Stauber
authors: Eckhard
authors: E
authors: Quandt
authors: Oliver
authors: O
authors: Hayden
mesh_terms: D000951:Antigens, Neoplasm / Q000737:chemistry / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry; D006801:Humans; D008280:Magnetics; D046210:Microfluidic Analytical Techniques; D013997:Time Factors
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule