title: In vitro evaluation of free radical scavenging activity of Codariocalyx motorius root extract.
abstract: OBJECTIVE
  To determine the phenolic content in Codariocalyx motorius root extract and to evaluate its antioxidant properties using various in vitro assay systems.
  
  
  METHODS
  The antioxidant activity was evaluated based on scavenging of 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radicals, superoxide anions, nitric oxide, hydrogen peroxide, peroxynitrite, reducing power and by inhibition of lipid peroxidation which was estimated in terms of thiobarbituric acid reactive substances.
  
  
  RESULTS
  The root extract of the Codariocalyx motorius (C. motorius) exhibited potent total antioxidant activity that increased with increasing amount of extract concentration, which was compared with standard drug such as quercetin, butylated hydroxytoluene, tocopherol at different concentrations. The different concentrations of the extracts showed inhibition on lipid peroxidation. In addition, the extracts had effective reducing power, free radical scavenging, super oxide anion scavenging, nitric oxide scavenging, lipid peroxidation, and total phenolic content depending on concentration. High correlation between total phenolic contents and scavenging potential of different reactive oxygen species (r(2)=0.831-0.978) indicated the polyphenols as the main antioxidants.
  
  
  CONCLUSIONS
  Codariocalyx motorius (C. motorius) root possess the highly active antioxidant substance which can be used for the treatment of oxidative stress-related diseases.
authors: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur-603 203, Tamilnadu, India.
authors: Uma
authors: U
authors: Chidambaram
authors: Vanitha
authors: V
authors: Pachamuthu
authors: Suganya
authors: S
authors: Natarajan
authors: Bhakkiyalakshmi
authors: B
authors: Elango
authors: Suriyanarayanan
authors: Kunga Mohan
authors: KM
authors: Ramkumar
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000494:pharmacology
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl