title: Liquid chromatography-tandem mass spectrometry-based plasma metabonomics delineate the effect of metabolites' stability on reliability of potential biomarkers.
abstract: Metabonomics is an important platform for investigating the metabolites of integrated living systems and their dynamic responses to changes caused by both endogenous and exogenous factors. A metabonomics strategy based on liquid chromatography-mass spectrometry/mass spectrometry in both positive and negative ion modes was applied to investigate the short-term and long-term stability of metabolites in plasma. Principal components analysis and ten types of identified metabolites were used to summarize the time-dependent change rules in metabolites systematically at different temperatures. The long-term stability of metabolites in plasma specimens stored at -80 °C for five years was also studied. Analysis of these subjects identified 36 metabolites with statistically significant changes in expression (p < 0.05) and found a kind of metabolite with a hundred-fold change. The stability of metabolites in blood at 4 °C for 24 h was also investigated. These studies show that a thorough understanding of the effects of metabolite stability are necessary for improving the reliability of potential biomarkers.
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, PR China.
authors: Wei
authors: W
authors: Yang
authors: Yanhua
authors: Y
authors: Chen
authors: Cong
authors: C
authors: Xi
authors: Ruiping
authors: R
authors: Zhang
authors: Yongmei
authors: Y
authors: Song
authors: Qimin
authors: Q
authors: Zhan
authors: Xiaofeng
authors: X
authors: Bi
authors: Zeper
authors: Z
authors: Abliz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis; D002853:Chromatography, Liquid; D008297:Male; D055432:Metabolomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D013696:Temperature; D013997:Time Factors
chemical_list: D015415:Biomarkers