title: The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis             under hypoxia and normoxia via multi-targeting activity.
abstract: The saponin monomer of dwarf lilyturf tuber, DT-13, exhibits anticancer             activity by reducing human breast cancer cell adhesion and migration under hypoxia.             To further investigate the anticancer activity of DT-13, we investigated whether             DT-13 exhibits anti-angiogenic activity. DT-13 showed no effect on human umbilical             vein endothelial cell proliferation but inhibited tube formation and migration             under normoxia and hypoxia. Moreover, DT-13 significantly reduced density of vessels             in vivo observed from a chicken chorioallantoic membrane model. Western blotting             results showed that DT-13 suppressed the increased level of hypoxia-inducible             factor 1α, p-extracellular signal-regulated kinase 1/2 and p-Akt induced by hypoxia.             Enzyme-linked immunosorbent assay revealed that vascular endothelial growth factor             excretion was suppressed by DT-13. DT-13 inhibited migration and tube formation             induced by vascular endothelial growth factor under normoxia and hypoxia. In addition,             DT-13 reduced the level of p-vascular endothelial growth factor receptor 2 and             p-Akt induced by vascular endothelial growth factor. Our data suggest that DT-13             inhibits angiogenesis under normoxia and hypoxia and also inhibits angiogenesis             induced by vascular endothelial growth factor via targeting at multi elements.
authors: Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, Jiangsu 210009, PR China.
authors: Renping
authors: R
authors: Zhao
authors: Li
authors: L
authors: Sun
authors: Sensen
authors: S
authors: Lin
authors: Xianshu
authors: X
authors: Bai
authors: Boyang
authors: B
authors: Yu
authors: Shengtao
authors: S
authors: Yuan
authors: Luyong
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000187:drug effects; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D031424:Liriope Plant / Q000737:chemistry; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012503:Saponins; D042461:Vascular Endothelial Growth Factor A