title: Stem cell metabolic and spectroscopic profiling.
abstract: Stem cells offer great potential for regenerative medicine because they regenerate damaged tissue by cell replacement and/or by stimulating endogenous repair mechanisms. Although stem cells are defined by their functional properties, such as the potential to proliferate, to self-renew, and to differentiate into specific cell types, their identification based on the expression of specific markers remains vague. Here, profiles of stem cell metabolism might highlight stem cell function more than the expression of single genes/markers. Thus, systematic approaches including spectroscopy might yield insight into stem cell function, identity, and stemness. We review the findings gained by means of metabolic and spectroscopic profiling methodologies, for example, nuclear magnetic resonance spectroscopy (NMRS), mass spectrometry (MS), and Raman spectroscopy (RS), with a focus on neural stem cells and neurogenesis.
authors: Institute of Biophysics and Physical Biochemistry, University of Regensburg, 93040 Regensburg, Germany.
authors: Paul
authors: P
authors: Ramm Sander
authors: Peter
authors: P
authors: Hau
authors: Steffen
authors: S
authors: Koch
authors: Karin
authors: K
authors: Schütze
authors: Ulrich
authors: U
authors: Bogdahn
authors: Hans Robert
authors: HR
authors: Kalbitzer
authors: Ludwig
authors: L
authors: Aigner
mesh_terms: D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D055432:Metabolomics / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods*; D013234:Stem Cells / Q000737:chemistry* / Q000378:metabolism*