title: Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors.
abstract: Cell therapy and cell-based tissue engineering is becoming increasingly important in regenerative medicine. Stem cells that are characterized by self-renewal, high proliferation and multiple differentiation potentials have attracted attention in cell-based regenerative medicine. Maintaining the aforementioned characteristics of stem cells is the first key step in cell-based regenerative medicine. Basic fibroblast growth factor (bFGF) is a well-known growth factor that efficiently maintains the self-renewal, high proliferation and multilineage differentiation potential of stem cells. Whether or not other growth factors, such as acidic fibroblast growth factor (aFGF) and epidermal growth factor (EGF) have similar effects has yet to be fully elucidated. Human hair follicle-derived mesenchymal stem cells (HF-MSCs) were obtained by organ culture. They exhibited surface markers of bone marrow mesenchymal stem cells as shown by positive staining for CD44, CD73, CD90 and CD105, and they also displayed trilineage differentiation potentials into adipocytes, chondrocytes and osteoblasts by cytochemistry and qRT-PCR. Flow cytometry analysis showed that up to 70% of HF-MSCs cultured in the presence of aFGF, bFGF or EGF stayed at the G0/G1 phase. Proliferation analysis showed that both bFGF and EGF at as low as 1 ng/ml and aFGF at above 5 ng/ml levels significantly increased the proliferation of HF-MSCs by cell counting. Consistent with proliferation analysis, immunofluorescence staining showed that more than 95% of HF-MSCs cultured in the presence of aFGF, bFGF and EGF were positively stained for proliferating cell nuclear antigen. HF-MSCs cultured in the presence of aFGF, bFGF or EGF retained marked trilineage differentiation potentials. By contrast, HF-MSCs cultured in the absence of bFGF, aFGF and EGF lost multipotency.
authors: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun, Jilin 130021, PR China.
authors: Xueyan
authors: X
authors: Zhang
authors: Yimei
authors: Y
authors: Wang
authors: Yunhe
authors: Y
authors: Gao
authors: Xuejuan
authors: X
authors: Liu
authors: Tingting
authors: T
authors: Bai
authors: Meiying
authors: M
authors: Li
authors: Lisha
authors: L
authors: Li
authors: Guanfan
authors: G
authors: Chi
authors: Hui
authors: H
authors: Xu
authors: Feilin
authors: F
authors: Liu
authors: Jin Yu
authors: JY
authors: Liu
authors: Yulin
authors: Y
authors: Li
mesh_terms: D000328:Adult; D015703:Antigens, CD; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: D015703:Antigens, CD; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor