title: A new hybrid signal amplification strategy for ultrasensitive electrochemical detection of DNA based on enzyme-assisted target recycling and DNA supersandwich assemblies.
abstract: A highly sensitive electrochemical sequence-specific DNA detection strategy is demonstrated by coupling N.BstNB I (a nicking endonuclease)-assisted target recycling amplification with DNA supersandwich assembly signal enhancement. The proposed method avoids any extra chemical labeling steps and offers high selectivity against single-base mismatch sequences and a low detection limit down to 0.36 fM.
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Ying
authors: Y
authors: Chen
authors: Qiong
authors: Q
authors: Wang
authors: Jin
authors: J
authors: Xu
authors: Yun
authors: Y
authors: Xiang
authors: Ruo
authors: R
authors: Yuan
authors: Yaqin
authors: Y
authors: Chai
mesh_terms: D020137:Base Pair Mismatch; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D016159:Tumor Suppressor Protein p53; D006046:Gold; D004247:DNA; C430823:endodeoxyribonuclease BstNBI; D015252:Deoxyribonucleases, Type II Site-Specific