title: Regulation of lipid biosynthesis, sliding motility, and biofilm formation by a membrane-anchored nucleoid-associated protein of Mycobacterium tuberculosis.
abstract: Bacteria use a number of small basic proteins for organization and compaction of their genomes. By their interaction with DNA, these nucleoid-associated proteins (NAPs) also influence gene expression. Rv3852, a NAP of Mycobacterium tuberculosis, is conserved among the pathogenic and slow-growing species of mycobacteria. Here, we show that the protein predominantly localizes in the cell membrane and that the carboxy-terminal region with the propensity to form a transmembrane helix is necessary for its membrane localization. The protein is involved in genome organization, and its ectopic expression in Mycobacterium smegmatis resulted in altered nucleoid morphology, defects in biofilm formation, sliding motility, and change in apolar lipid profile. We demonstrate its crucial role in regulating the expression of KasA, KasB, and GroEL1 proteins, which are in turn involved in controlling the surface phenotypes in mycobacteria.
authors: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
authors: Soumitra
authors: S
authors: Ghosh
authors: Shantinath S
authors: SS
authors: Indi
authors: Valakunja
authors: V
authors: Nagaraja
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D016680:Genome, Bacterial; D008055:Lipids / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000378:metabolism*; D009068:Movement; D020102:Mycobacterium smegmatis / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D008055:Lipids; D008565:Membrane Proteins
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