title: Activation of platelets upon contact with a vitamin E-coated/non-coated surface.
abstract: The purpose of this study was to determine the effects of a vitamin E-coated surface on platelet activation, focusing on the interactions among the vitamin E-coated surface, platelets and leukocytes. Platelet-rich plasma (PRP) or PRP containing leukocytes (LPRP) was used. No difference was observed in platelet activation between PRP and LPRP for a vitamin E-coated membrane, meaning that platelet activation triggered by leukocytes was suppressed in plasma coming in contact with a vitamin E-coated membrane, while the membrane itself directly induced platelet activation. The antioxidant capacity of the vitamin E-coated membrane in contact with PRP or LPRP was partially reduced, but sufficient residual capacity remained. The in vitro experiments using an oxidized vitamin E-coated surface revealed that P-selectin expression and superoxide anion production in the platelets and platelet adhesion were induced by contact with the oxidized vitamin E-coated surface. We conclude that contact with a vitamin E-coated surface reduces platelet activation mediated by superoxide anions, probably by reducing superoxide anions, but during the process of the reduction, the vitamin E-coated surface itself becomes oxidized, which again causes platelet activation. The beneficial effects of a vitamin E-coated dialyzer in respect of platelet activation were counteracted by the formation of oxidized vitamin E.
authors: Kitasato University Graduate School of Medical Sciences, Kanagawa, Japan.
authors: Hiroshi
authors: H
authors: Tsukao
authors: Kenichi
authors: K
authors: Kokubo
authors: Haruko
authors: H
authors: Takahashi
authors: Mina
authors: M
authors: Nagasato
authors: Takanori
authors: T
authors: Endo
authors: Naoto
authors: N
authors: Iizuka
authors: Toshihiro
authors: T
authors: Shinbo
authors: Minoru
authors: M
authors: Hirose
authors: Hirosuke
authors: H
authors: Kobayashi
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects; D008567:Membranes, Artificial; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010084:Oxidation-Reduction; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation / Q000187:drug effects*; D053657:Platelet-Rich Plasma / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000032:analysis; D006435:Renal Dialysis / Q000295:instrumentation*; D018709:Statistics, Nonparametric; D013481:Superoxides / Q000032:analysis; D013552:Swine; D014810:Vitamin E / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D019007:P-Selectin; D017382:Reactive Oxygen Species; D013481:Superoxides; D014810:Vitamin E; D009240:N-Formylmethionine Leucyl-Phenylalanine; D014969:Xanthine Oxidase
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