title: A reliable way to detect endogenous murine β-amyloid.
abstract: Unraveling the normal physiologic role of β-amyloid is likely crucial to understanding the pathogenesis of Alzheimer's disease. However, progress on this question is currently limited by the high background of many ELISAs for murine β-amyloid. Here, we examine the background signal of several murine β-amyloid ELISAs, and conclude that the majority of the background is from non-APP derived proteins. Most importantly, we identify ELISAs that eliminate this background signal.
authors: Department of Pathology and Cell Biology, Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University, New York, New York, United States of America. aft25@columbia.edu
authors: Andrew F
authors: AF
authors: Teich
authors: Mitesh
authors: M
authors: Patel
authors: Ottavio
authors: O
authors: Arancio
mesh_terms: D000544:Alzheimer Disease / Q000503:physiopathology*; D016229:Amyloid beta-Peptides / Q000302:isolation & purification*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006624:Hippocampus / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor
references: Cell. 1995 May 19;81(4):525-31
references: 7758106
references: Nat Neurosci. 2009 Dec;12(12):1567-76
references: 19935655
references: Cell. 2006 Aug 25;126(4):775-88
references: 16923396
references: Exp Neurol. 1999 Aug;158(2):328-37
references: 10415140
references: PLoS One. 2009 Sep 22;4(9):e7134
references: 19771166
references: J Neurosci. 2003 Jul 2;23(13):5531-5
references: 12843253
references: J Neurochem. 2004 Sep;90(6):1359-70
references: 15341520
references: Ann Neurol. 2011 May;69(5):819-30
references: 21472769
references: J Neurosci Methods. 2008 Mar 30;169(1):16-22
references: 18160105
references: Trends Pharmacol Sci. 1991 Oct;12(10):383-8
references: 1763432
references: Biochem Pharmacol. 2005 Feb 15;69(4):689-98
references: 15670587
references: J Neurosci. 2008 Dec 31;28(53):14537-45
references: 19118188
references: Science. 1996 Oct 4;274(5284):99-102
references: 8810256
references: J Pharmacol Exp Ther. 2005 May;313(2):902-8
references: 15743924
references: J Neurosci. 2003 Oct 1;23(26):8844-53
references: 14523085
references: J Neurosci. 2004 Jun 9;24(23):5439-44
references: 15190117
references: J Neurosci Methods. 2006 Oct 15;157(1):71-81
references: 16678274