title: Highly efficient production of L-lactic acid from xylose by newly isolated Bacillus coagulans C106.
abstract: Cost-effective production of optically pure lactic acid from lignocellulose sugars is commercially attractive but challenging. Bacillus coagulans C106 was isolated from environment and used to produce l-lactic acid from xylose at 50°C and pH 6.0 in mineral salts medium containing 1-2% (w/v) of yeast extract without sterilizing the medium before fermentation. In batch fermentation with 85g/L of xylose, lactic acid titer and productivity reached 83.6g/L and 7.5g/Lh, respectively. When fed-batch (120+80+60g/L) fermentation was applied, they reached 215.7g/L and 4.0g/Lh, respectively. In both cases, the lactic acid yield and optical purity reached 95% and 99.6%, respectively. The lactic acid titer and productivity on xylose are the highest among those ever reported. Ca(OH)2 was found to be a better neutralizing agent than NaOH in terms of its giving higher lactic acid titer (1.2-fold) and productivity (1.8-fold) under the same conditions.
authors: Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research, 1 Pesek Road, Jurong Island, Singapore 627833, Singapore.
authors: Lidan
authors: L
authors: Ye
authors: Xingding
authors: X
authors: Zhou
authors: Mohammad Sufian Bin
authors: MS
authors: Hudari
authors: Zhi
authors: Z
authors: Li
authors: Jin Chuan
authors: JC
authors: Wu
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D002126:Calcium Hydroxide; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005285:Fermentation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D017422:Sequence Analysis, DNA; D014994:Xylose / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D019344:Lactic Acid; D014994:Xylose; D007770:L-Lactate Dehydrogenase; D002126:Calcium Hydroxide