title: [Role of INGAP-pp in the differentiation of hUCMSCs into insulin producing cells].
abstract: OBJECTIVE
  To find out a protocol for the efficient and directional differentiation from human umbilical cord mesenchymal stem cells (hUCMSCs) into insulin-producing cells (IPCs).
  
  
  METHODS
  HUCMSCs were induced to differentiate into IPCs by an improved protocol based on a traditional protocol with addition of islet neogenesis-associated protein-pp (INGAP-pp), and the IPCs were identified by morphological observation, flow cytometry, real-time quantitative PCR, immunofluorescence and ELISA.
  
  
  RESULTS
  Compared with the traditional protocol, the improved protocol resulted in the higher rate of C peptide positive cells (P<0.05) and the increased mRNA expressions of pancreas development related transcription factor PDX-1, Ngn3, NeuroD1, MafA, insulin, Glut-2 (P<0.05). Meanwhile, by the improved protocol the protein expressions of C peptide, PDX-1 and Nkx6.1 were positive, and insulin and C-peptide release were promoted (P<0.05) as demonstrated by glucose challenge test.
  
  
  CONCLUSIONS
  INGAP-pp may facilitate the differentiation of hUCMSCs into IPCs. However, the IPCs are not as mature as normal human islet β cells.
authors: Department of Rheumatism, Jinan University, Shenzhen, China. s_tchen32163.com
authors: Tao
authors: T
authors: Chen
authors: Yunshuai
authors: Y
authors: Wang
authors: Lehui
authors: L
authors: Gu
authors: Shuyan
authors: S
authors: Zhou
authors: Jian
authors: J
authors: Ye
authors: Youqiang
authors: Y
authors: Jian
authors: Furong
authors: F
authors: Li
authors: Hui
authors: H
authors: Qi
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002454:Cell Differentiation; D006801:Humans; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human