title: [Adenovirus-mediated canine interferon-gamma expression and its antiviral activity against canine parvovirus].
abstract: OBJECTIVE
  To construct recombinant adenovirus containing canine interferon-gamma (cIFN-gamma) gene and to investigate its antiviral activity against canine parvovirus in Madin-Darby canine kidney cells (MDCK). [Methods] The cIFN-gamma gene was inserted into adenovirus shuttle plasmid to construct pShuttle3-cIFN-gamma expression vector, from which the cIFN-gamma expression cassette was transferred into the adenovirus genomic plasmid pAdeno-X by specific restriction sites to generate recombinant adenovirus genomic plasmid pAd-cIFN-gamma. The pAd-cIFN-gamma plasmid was linearized by digestion and transfected into human embryonic kidney (HEK) 293T cells to generate the replication-defective cIFN-gamma recombinant adenovirus (Ad-cIFN-gamma). To analyze its anti-canine parvovirus activity, the MDCK cells were pre-infected by Ad-cIFN-gamma recombinant adenovirus, and then infected by canine parvovirus. The antiviral activity of the Ad-cIFN-gamma recombinant adenovirus against parvovirus was analyzed.
  
  
  RESULTS
  The recombinant adenovirus containing cIFN-gamma gene was constructed by the ligation method. The recombinant adenovirus could mediates recombinant cIFN-gamma secretory expression in MDCK cells. The Ad-cIFN-gamma recombinant adenovirus could significantly inhibit canine parvovirus replication in MDCK cells pre-infected with the recombinant adenovirus. These results indicate that the Ad-cIFN-gamma recombinant adenovirus has the potent antiviral activity against canine parvovirus.
  
  
  CONCLUSIONS
  The Ad-cIFN-gamma recombinant adenovirus was successfully constructed by the ligation method and possessed a powerful antiviral activity against canine parvovirus.
authors: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Agricultural University of Hebei, North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding 071001, China. zhangkao0110@163.com
authors: Kao
authors: K
authors: Zhang
authors: Huijun
authors: H
authors: Jin
authors: Fei
authors: F
authors: Zhong
authors: Xiujin
authors: X
authors: Li
authors: Changai
authors: C
authors: Neng
authors: Huihui
authors: H
authors: Chen
authors: Wenyan
authors: W
authors: Li
authors: Jiexia
authors: J
authors: Wen
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D004285:Dogs; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology*; D010322:Parvoviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics / Q000276:immunology*
chemical_list: D007371:Interferon-gamma