title: The involvement of annexin II in resistance to UVB-induced cell death and in the increased nucleotide excision repair capacity of UV-damaged DNA in human cells.
abstract: Annexin II, an HSP27-interacting protein, is involved in the protection of human cells against UVC. UVB is concerned with deleterious actions on human health. In this study, we attempted to confirm the anti-UVB effect of annexin II, and to elucidate the mechanisms underlying annexin II-involving UV resistance. The RSa cells were more sensitive to UVB lethality than the AP(r)-1 cells. Overproduction of annexin II in RSa cells resulted in increased resistance to UVB lethality, while annexin II siRNA-transfected AP(r)-1 cells were sensitized to UVB lethality. The excision capacity of the two major types (CPD and 6-4PP) of UVC- and UVB-damaged DNA in AP(r)-1 cells was greater than in RSa cells. The excision capacity of the RSa cells improved following upregulation of annexin II, while the capacity of the AP(r)-1 cells decreased after annexin II downregulation. Our results suggest that annexin II is involved in the UV resistance of human cells, via functioning in nucleotide excision repair.
authors: Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors: Azuha
authors: A
authors: Yano
authors: Shi-Ping
authors: SP
authors: Chen
authors: Kazuko
authors: K
authors: Kita
authors: Xia
authors: X
authors: Jiang
authors: Qian
authors: Q
authors: Ren
authors: Tetsuo
authors: T
authors: Sato
authors: Nobuo
authors: N
authors: Suzuki
mesh_terms: D017306:Annexin A2 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D016923:Cell Death / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D018832:Molecular Chaperones; D009928:Organ Specificity; D034741:RNA, Small Interfering / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics*; D015398:Signal Transduction / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D017306:Annexin A2; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D034741:RNA, Small Interfering; D004247:DNA