title: HsfA1d, a protein identified via FOX hunting using Thellungiella salsuginea cDNAs improves heat tolerance by regulating heat-stress-responsive gene expression.
abstract: Thellungiella salsuginea (formerly T. halophila), a species closely related to Arabidopsis (Arabidopsis thaliana), is tolerant not only to high salt levels, but also to chilling, freezing, and ozone. Here, we report that T. salsuginea also shows greater heat tolerance than Arabidopsis. We identified T. salsuginea HsfA1d (TsHsfA1d) as a gene that can confer marked heat tolerance on Arabidopsis. TsHsfA1d was identified via Full-length cDNA Over-eXpressing gene (FOX) hunting from among a collection of heat-stress-related T. salsuginea cDNAs. Transgenic Arabidopsis overexpressing TsHsfA1d showed constitutive up-regulation of many genes in the Arabidopsis AtHsfA1 regulon under normal growth temperature. In Arabidopsis mesophyll protoplasts, TsHsfA1d was localized in both the nucleus and the cytoplasm. TsHsfA1d also interacted with AtHSP90, which negatively regulates AtHsfA1s by forming HsfA1-HSP90 complexes in the cytoplasm. It is likely that the partial nuclear localization of TsHsfA1d induced the expression of the AtHsfA1d regulon in the transgenic plants at normal temperature. We also discovered that transgenic Arabidopsis plants overexpressing AtHsfA1d were more heat-tolerant than wild-type plants and up-regulated the expression of the HsfA1d regulon, as was observed in TsHsfA1d-overexpressing plants. We propose that the products of both TsHsfA1d and AtHsfA1d function as positive regulators of Arabidopsis heat-stress response and would be useful for the improvement of heat-stress tolerance in other plants.
authors: Department of Bio-Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
authors: Yukari
authors: Y
authors: Higashi
authors: Naohiko
authors: N
authors: Ohama
authors: Tomoko
authors: T
authors: Ishikawa
authors: Taku
authors: T
authors: Katori
authors: Ayaka
authors: A
authors: Shimura
authors: Kazuya
authors: K
authors: Kusakabe
authors: Kazuko
authors: K
authors: Yamaguchi-Shinozaki
authors: Junko
authors: J
authors: Ishida
authors: Maho
authors: M
authors: Tanaka
authors: Motoaki
authors: M
authors: Seki
authors: Kazuo
authors: K
authors: Shinozaki
authors: Yoichi
authors: Y
authors: Sakata
authors: Takahisa
authors: T
authors: Hayashi
authors: Teruaki
authors: T
authors: Taji
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D042541:Intracellular Space / Q000378:metabolism; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins