title: Oligopeptidases B from Trypanossoma cruzi and Trypanossoma brucei inhibit inflammatory pain in mice by targeting serotoninergic receptors.
abstract: In the present study, the antinociceptive profile of oligopeptidases B from Trypanosoma cruzi (OPTc) and Trypanosoma brucei (OPTb) were examined in mice evaluated by the acetic acid-induced writhing test. Both OPTc and OPTb injected intraperitoneally attenuated the writhing numbers in the acetic acid-induced writhing test. This effect was not dependent on the enzymatic activity, but the enzyme structure was important for this purpose. Intraperitoneal pretreatment with methysergide (5-HT serotonergic receptor antagonist) attenuated antinociceptive effect induced by both OPTc and OPTb in the writhing test. However, naloxone (opioid receptor antagonist) or yohimbine (α2-adrenergic receptor antagonist) did not affect antinociception induced by both oligopeptidases. Our results suggest that OPTc and OPTb show antinociceptive property in the writhing test. Furthermore, this antinociceptive effect may be mediated by serotonergic receptor but not opioidergic or α2-adrenergic receptors.
authors: Laboratory of Neuromodulation and Experimental Pain, Hospital Sírio-Libanês, São Paulo, Brazil.
authors: Rafaela Quintanilha
authors: RQ
authors: Abrahão
authors: Adriano Cardoso
authors: AC
authors: Franciosi
authors: Douglas
authors: D
authors: Andrade
authors: Luiz
authors: L
authors: Juliano
authors: Maria Aparecida
authors: MA
authors: Juliano
authors: Renata
authors: R
authors: Giorgi
authors: Camila Squarzoni
authors: CS
authors: Dale
mesh_terms: D058669:Adrenergic alpha-2 Receptor Antagonists / Q000494:pharmacology; D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D008297:Male; D008784:Methysergide; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement / Q000187:drug effects; D011985:Receptors, Serotonin / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000008:administration & dosage / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000201:enzymology*; D015016:Yohimbine / Q000494:pharmacology
chemical_list: D058669:Adrenergic alpha-2 Receptor Antagonists; D000700:Analgesics; D009292:Narcotic Antagonists; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D015016:Yohimbine; D009270:Naloxone; D012697:Serine Endopeptidases; C090703:oligopeptidase B; D008784:Methysergide
references: Exp Parasitol. 1978 Jun;45(1):8-18
references: 352716
references: J Cell Biol. 1997 Feb 10;136(3):609-20
references: 9024691
references: Br J Pharmacol Chemother. 1968 Feb;32(2):295-310
references: 4230818
references: Eur J Anaesthesiol. 1984 Jun;1(2):171-99
references: 6152613
references: Nucleic Acids Res. 2008 Jan;36(Database issue):D320-5
references: 17991683
references: PLoS One. 2012;7(1):e30431
references: 22276197
references: Biochim Biophys Acta. 2009 Nov;1794(11):1673-83
references: 19665591
references: J Mol Graph Model. 2000 Feb;18(1):7-17, 57-8
references: 10935201
references: J Pharmacol Exp Ther. 1984 Jan;228(1):1-12
references: 6319664
references: Infect Immun. 2001 Apr;69(4):2757-61
references: 11254649
references: J Biochem. 2010 Feb;147(2):201-11
references: 19819899
references: J Biochem. 1984 Apr;95(4):1169-77
references: 6378896
references: Brain Res. 1979 Jan 5;160(1):180-5
references: 581478
references: Arch Biochem Biophys. 1992 Feb 14;293(1):25-31
references: 1731636
references: Parasitol Res. 2007 Sep;101(4):865-75
references: 18074461
references: J Biochem. 2004 Nov;136(5):673-81
references: 15632308
references: J Pharmacol Exp Ther. 1969 Sep;169(1):29-38
references: 5804610
references: Brain Res. 1984 Nov 12;321(2):287-97
references: 6149792
references: FEBS Lett. 1998 Aug 21;433(3):251-6
references: 9744805
references: Biochimie. 2008 Feb;90(2):336-44
references: 18029266
references: Pain. 1983 Jun;16(2):109-110
references: 6877845
references: J Biol Chem. 1995 Mar 10;270(10):5172-80
references: 7890627
references: Eur J Biochem. 1996 Jun 15;238(3):728-36
references: 8706674
references: J Biol Chem. 2005 Mar 25;280(12):10925-37
references: 15644339
references: Cell Microbiol. 2002 Nov;4(11):701-11
references: 12427093
references: J Biol Chem. 1975 Oct 10;250(19):7771-9
references: 240839
references: Int J Tissue React. 1985;7(1):61-7
references: 3891654
references: Biochem Pharmacol. 2000 Nov 15;60(10):1497-504
references: 11020452
references: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt 8):808-10
references: 16880564
references: EMBO J. 1998 Sep 1;17(17):4975-86
references: 9724634
references: J Pharmacol Exp Ther. 1987 Jul;242(1):90-5
references: 3612540
references: Proteins. 1997 Jul;28(3):375-9
references: 9223183
references: Parasitol Res. 1992;78(7):553-6
references: 1438145