title: Sensitive amperometric biosensor for phenolic compounds based on graphene-silk peptide/tyrosinase composite nanointerface.
abstract: New graphene-silk peptide (Gr-SP) nanosheets were prepared and successfully fabricated with tyrosinase (Tyr) as a novel biosensor for the determination of phenolic compounds. The Gr-SP nanosheets were fully characterized with transmission electron microscopy, x-ray diffraction, x-ray photoelectron spectroscopy, UV/Vis and FTIR spectra. The developed biosensors were also characterized with scanning electronic microscopy and electrochemical impedance spectroscopy. Using bisphenol A (BPA) as a model substrate in the sensing system, a number of key factors including the volume of Gr-SP-Tyr solution, the applied potential, pH values, temperature, and the Tyr/Gr-SP ratio that influence the analytical performance of the biosensor were investigated. The biosensor gave a linear response on the concentration ranges of 0.001-16.91 μM for catechol with the sensitivity of 7634 mA M(-1)cm(-2), 0.0015-21.12 μM for phenol with the sensitivity of 4082 mA M(-1)cm(-2), and 0.002-5.48 μM for BPA with the sensitivity of 2511 mA M(-1)cm(-2). The low detection limits were estimated to be 0.23, 0.35 and 0.72 nM (S/N=3) for catechol, phenol and BPA, respectively. The biosensors also exhibit good repeatability and long-term stability. The practical application of the biosensor was also demonstrated by the determination of BPA leaching from commercial plastic drinking bottles.
authors: Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, Hubei 430074, China.
authors: Ying
authors: Y
authors: Qu
authors: Ming
authors: M
authors: Ma
authors: Zhengguo
authors: Z
authors: Wang
authors: Guoqing
authors: G
authors: Zhan
authors: Buhai
authors: B
authors: Li
authors: Xian
authors: X
authors: Wang
authors: Huaifang
authors: H
authors: Fang
authors: Huijuan
authors: H
authors: Zhang
authors: Chunya
authors: C
authors: Li
mesh_terms: D000395:Air Pollutants, Occupational / Q000032:analysis / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002396:Catechols / Q000032:analysis / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D047011:Silk / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D002396:Catechols; D004800:Enzymes, Immobilized; D006108:Graphite; D014442:Monophenol Monooxygenase; D010455:Peptides; D010636:Phenols; D047011:Silk; C034221:catechol; C006780:bisphenol A; D000098826:Bisphenol A Compounds