title: Chromoplast-specific carotenoid-associated protein appears to be important for enhanced accumulation of carotenoids in hp1 tomato fruits.
abstract: Tomato (Solanum lycopersicum) high-pigment mutants with lesions in diverse loci such as DNA Damage-Binding Protein1 (high pigment1 [hp1]), Deetiolated1 (hp2), Zeaxanthin Epoxidase (hp3), and Intense pigment (Ip; gene product unknown) exhibit increased accumulation of fruit carotenoids coupled with an increase in chloroplast number and size. However, little is known about the underlying mechanisms exaggerating the carotenoid accumulation and the chloroplast number in these mutants. A comparison of proteome profiles from the outer pericarp of hp1 mutant and wild-type (cv Ailsa Craig) fruits at different developmental stages revealed at least 72 differentially expressed proteins during ripening. Hierarchical clustering grouped these proteins into three clusters. We found an increased abundance of chromoplast-specific carotenoid-associated protein (CHRC) in hp1 fruits at red-ripe stage that is also reflected in its transcript level. Western blotting using CHRC polyclonal antibody from bell pepper (Capsicum annuum) revealed a 2-fold increase in the abundance of CHRC protein in the red-ripe stage of hp1 fruits compared with the wild type. CHRC levels in hp2 were found to be similar to that of hp1, whereas hp3 and Ip showed intermediate levels to those in hp1, hp2, and wild-type fruits. Both CHRC and carotenoids were present in the isolated plastoglobules. Overall, our results suggest that loss of function of DDB1, DET1, Zeaxanthin Epoxidase, and Ip up-regulates CHRC levels. Increase in CHRC levels may contribute to the enhanced carotenoid content in these high-pigment fruits by assisting in the sequestration and stabilization of carotenoids.
authors: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors: Himabindu Vasuki
authors: HV
authors: Kilambi
authors: Rakesh
authors: R
authors: Kumar
authors: Rameshwar
authors: R
authors: Sharma
authors: Yellamaraju
authors: Y
authors: Sreelakshmi
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D015153:Blotting, Western; D002338:Carotenoids / Q000096:biosynthesis / Q000378:metabolism*; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005638:Fruit / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D018087:Plastids / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D049490:Systems Biology
chemical_list: D010940:Plant Proteins; D020543:Proteome; D002338:Carotenoids
references: J Proteome Res. 2009 May;8(5):2601-7
references: 19290622
references: Nucleic Acids Res. 2004 Oct 14;32(18):5539-45
references: 15486203
references: Ann Bot. 2011 Aug;108(2):291-7
references: 21788376
references: Trends Plant Sci. 1999 Jun;4(6):232-235
references: 10366880
references: Plant Cell. 1999 Feb;11(2):145-57
references: 9927635
references: Nat Biotechnol. 2005 Jul;23(7):890-5
references: 15951803
references: Biotechnol Adv. 2010 Jan-Feb;28(1):108-18
references: 19850117
references: Plant Physiol. 2012 Mar;158(3):1139-45
references: 22253257
references: Phytochemistry. 2007 Jun;68(11):1545-56
references: 17466343
references: J Exp Bot. 2010 May;61(9):2413-31
references: 20363867
references: Plant Physiol. 2012 Mar;158(3):1172-92
references: 22274653
references: Theor Appl Genet. 2003 Feb;106(3):454-60
references: 12589545
references: Plant Cell Physiol. 2006 Dec;47(12):1663-73
references: 17098784
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Plant Biol (Stuttg). 2011 May;13(3):546-50
references: 21489107
references: Bioinformatics. 2005 Apr 1;21(7):1280-1
references: 15546938
references: Theor Appl Genet. 2004 May;108(8):1574-81
references: 14968305
references: Plant J. 2006 Aug;47(4):591-603
references: 16792691
references: Biochem Biophys Res Commun. 1994 Mar 30;199(3):1144-50
references: 8147854
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9897-902
references: 15178762
references: J Agric Food Chem. 2012 Feb 22;60(7):1764-75
references: 22264157
references: Plant Physiol. 2007 Mar;143(3):1327-46
references: 17208958
references: Plant Physiol. 2007 Oct;145(2):389-401
references: 17704236
references: Plant Physiol. 1998 Jul;117(3):1047-58
references: 9662548
references: Plant Physiol. 1997 Nov;115(3):1185-94
references: 9390444
references: Plant J. 2008 Jul;55(1):89-103
references: 18363785
references: Plant Physiol. 2004 Feb;134(2):790-800
references: 14739347
references: J Exp Bot. 2011 Nov;62(15):5297-309
references: 21841170
references: Plant Physiol. 2006 Sep;142(1):233-44
references: 16815957
references: Plant J. 2011 Dec;68(6):999-1013
references: 21851430
references: Photochem Photobiol. 2008 Nov-Dec;84(6):1388-94
references: 19067960
references: Plant J. 2012 Apr;70(2):243-55
references: 22098335
references: Methods Mol Biol. 2011;775:223-39
references: 21863446
references: Plant Physiol. 1993 Jun;102(2):491-496
references: 12231837
references: Plant Cell. 2010 Apr;22(4):1190-215
references: 20435899
references: Biochem Soc Symp. 1994;60:249-56
references: 7639784
references: J Exp Bot. 2011 May;62(8):2797-813
references: 21330356
references: Plant Physiol. 2011 Jul;156(3):1424-38
references: 21571667
references: Plant Cell. 2010 Jan;22(1):108-23
references: 20061554
references: Biotechnol Annu Rev. 2008;14:171-90
references: 18606363
references: Plant Cell. 1993 Apr;5(4):379-87
references: 8485401
references: Plant Physiol. 2009 Mar;149(3):1505-28
references: 19144766
references: Plant Physiol. 2006 Mar;140(3):984-97
references: 16461379
references: Planta. 2003 Oct;217(6):896-903
references: 12844264
references: Mol Plant. 2012 Mar;5(2):339-52
references: 22155949
references: Plant Cell. 2006 Dec;18(12):3594-605
references: 17172359
references: Plant Physiol. 2011 Sep;157(1):405-25
references: 21795583
references: Annu Rev Genet. 2011;45:41-59
references: 22060040
references: Planta. 2010 Aug;232(3):555-66
references: 20499085
references: Biochim Biophys Acta. 2000 Oct 18;1482(1-2):84-91
references: 11058750
references: Plant Cell Physiol. 2011 May;52(5):851-65
references: 21450689
references: Plant J. 2008 Mar;53(5):717-30
references: 17988221
references: J Exp Bot. 2008;59(11):2979-90
references: 18550598
references: J Proteomics. 2009 May 2;72(4):586-607
references: 19135558
references: Nat Protoc. 2006;1(6):2856-60
references: 17406544
references: Nat Protoc. 2006;1(2):769-74
references: 17406306
references: Plant Cell. 2006 Jul;18(7):1693-703
references: 16731586
references: Anal Biochem. 1996 Nov 15;242(2):197-201
references: 8937562
references: Plant Cell. 2007 Oct;19(10):3194-211
references: 17933904
references: Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4
references: 388439
references: J Agric Food Chem. 2009 Jun 10;57(11):4818-26
references: 19391624
references: FEBS Lett. 2002 Apr 24;517(1-3):129-32
references: 12062422
references: J Exp Bot. 2006;57(9):1883-97
references: 16449380
references: Plant Physiol. 1994 Feb;104(2):321-326
references: 12232083
references: Trends Plant Sci. 2008 Jan;13(1):36-43
references: 18160330
references: Proteomics. 2006 Jul;6(13):3781-91
references: 16739135