title: Differential expression of exosomal microRNAs in prefrontal cortices of schizophrenia and bipolar disorder patients.
abstract: Exosomes are cellular secretory vesicles containing microRNAs (miRNAs). Once secreted, exosomes are able to attach to recipient cells and release miRNAs potentially modulating the function of the recipient cell. We hypothesized that exosomal miRNA expression in brains of patients diagnosed with schizophrenia (SZ) and bipolar disorder (BD) might differ from controls, reflecting either disease-specific or common aberrations in SZ and BD patients. The sources of the analyzed samples included McLean 66 Cohort Collection (Harvard Brain Tissue Resource Center), BrainNet Europe II (BNE, a consortium of 18 brain banks across Europe) and Boston Medical Center (BMC). Exosomal miRNAs from frozen postmortem prefrontal cortices with well-preserved RNA were isolated and submitted to profiling by Luminex FLEXMAP 3D microfluidic device. Multiple statistical analyses of microarray data suggested that certain exosomal miRNAs were differentially expressed in SZ and BD subjects in comparison to controls. RT-PCR validation confirmed that two miRNAs, miR-497 in SZ samples and miR-29c in BD samples, have significantly increased expression when compared to control samples. These results warrant future studies to evaluate the potential of exosome-derived miRNAs to serve as biomarkers of SZ and BD.
authors: Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America.
authors: Meredith G
authors: MG
authors: Banigan
authors: Patricia F
authors: PF
authors: Kao
authors: James A
authors: JA
authors: Kozubek
authors: Ashley R
authors: AR
authors: Winslow
authors: Juan
authors: J
authors: Medina
authors: Joan
authors: J
authors: Costa
authors: Andrea
authors: A
authors: Schmitt
authors: Anja
authors: A
authors: Schneider
authors: Howard
authors: H
authors: Cabral
authors: Ozge
authors: O
authors: Cagsal-Getkin
authors: Charles R
authors: CR
authors: Vanderburg
authors: Ivana
authors: I
authors: Delalle
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D001714:Bipolar Disorder / Q000235:genetics* / Q000378:metabolism; D055354:Exosomes / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D017397:Prefrontal Cortex / Q000378:metabolism*; D012559:Schizophrenia / Q000235:genetics* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
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