title: A role of Sema6A expressed in oligodendrocyte precursor cells.
abstract: Our previous study has confirmed that the distribution of oligodendrocyte precursor cells (OPCs) is disturbed in the embryonic cerebral cortex of Plexin-A4 knockout mice, and that Sema6A is expressed in OPCs in the region. The present study examined whether Sema6A expressed in OPCs is involved in their own migration, and used a clonal FBD-102b line as OPCs model. In an in vitro migration assay, Sema6A knockdown repressed the migration of FBD-102b cells. Additionally, in co-culture, 3T3 cells ectopically expressing Plexin-A4 were segregated from 3T3 cells ectopically expressing Sema6A. When FBD-102b cells were seeded in a spot and exposed to a gradient of both Sema3A and Sema6A, dispersion of FBD-102b cells was suppressed, and Plexin-A4 knockdown in FBD-102b cells attenuated the suppressive effect of the Semaphorins. These results indicate that Sema6A expressed in OPCs is involved in their autonomous migration through ligand-receptor interaction with Plexin-A4 expressed in surrounding cells.
authors: Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors: Atsumasa
authors: A
authors: Okada
authors: Yasuhiro
authors: Y
authors: Tomooka
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D002999:Clone Cells; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D051379:Mice; D008817:Mice, Mutant Strains; D041681:NIH 3T3 Cells; D009419:Nerve Tissue Proteins; D009836:Oligodendroglia / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D040121:Semaphorin-3A / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; C492110:Plxna4 protein, mouse; D011956:Receptors, Cell Surface; C488217:Sema3a protein, mouse; C504203:Sema6a protein, mouse; D040121:Semaphorin-3A; D039961:Semaphorins