title: Curcumin restores diabetes induced neurochemical changes in the brain stem of Wistar rats.
abstract: Diabetes mellitus, when poorly controlled, leads to debilitating central nervous system (CNS) complications including cognitive deficits, somatosensory and motor dysfunction. The present study investigated curcumin's potential in modulating diabetes induced neurochemical changes in brainstem. Expression analysis of cholinergic, insulin receptor and GLUT-3 in the brainstem of streptozotocin (STZ) induced diabetic rats were studied. Radioreceptor binding assays, gene expression studies and immunohistochemical analysis were done in the brainstem of male Wistar rats. Our result showed that Bmax of total muscarinic and muscarinic M3 receptors were increased and muscarinic M1 receptor was decreased in diabetic rats compared to control. mRNA level of muscarinic M3, α7-nicotinic acetylcholine, insulin receptors, acetylcholine esterase, choline acetyltransferase and GLUT-3 significantly increased and M1 receptor decreased in the brainstem of diabetic rats. Curcumin and insulin treatment restored the alterations and maintained all parameters to near control. The results show that diabetes is associated with significant reduction in brainstem function coupled with altered cholinergic, insulin receptor and GLUT-3 gene expression. The present study indicates beneficial effect of curcumin in diabetic rats by regulating the cholinergic, insulin receptor and GLUT-3 in the brainstem similar to the responses obtained with insulin therapy.
authors: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Cochin University of Science and Technology, Cochin 682022, Kerala, India. peeyush.chembio@gmail.com
authors: Peeyush T
authors: PT
authors: Kumar
authors: Naijil
authors: N
authors: George
authors: Sherin
authors: S
authors: Antony
authors: Cheramadathikudiyil Skaria
authors: CS
authors: Paulose
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics; D000818:Animals; D001933:Brain Stem / Q000187:drug effects* / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000235:genetics; D003474:Curcumin / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D051274:Glucose Transporter Type 3 / Q000235:genetics; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000235:genetics; D011976:Receptors, Muscarinic / Q000378:metabolism; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000378:metabolism
chemical_list: D051274:Glucose Transporter Type 3; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D011976:Receptors, Muscarinic; C495690:Slc2a3 protein, rat; D064569:alpha7 Nicotinic Acetylcholine Receptor; D002795:Choline O-Acetyltransferase; D011972:Receptor, Insulin; D000110:Acetylcholinesterase; D003474:Curcumin