title: Effects of coronary ischemia-reperfusion in a rat model of early overnutrition. Role of angiotensin receptors.
abstract: BACKGROUND
  Obesity during childhood has dramatically increased worldwide in the last decades. Environmental factors acting early in life, including nutrition, play an important role in the pathogenesis of obesity and cardiovascular diseases in adulthood.
  
  
  OBJECTIVE
  To analyze the effects of early overfeeding on the heart and coronary circulation, the effect of ischemia-reperfusion (I/R) and the role of the renin-angiotensin system (RAS) were studied in isolated hearts from control and overfed rats during lactation.
  
  
  RESULTS
  On the day of birth litters were adjusted to twelve pups per mother (control) or to three pups per mother (overfed). At weaning (21 days) the rats were killed and the heart perfused in a Langendorff system and subjected to 30 min of ischemia followed by 15 min of reperfusion. The contractility (left developed intraventricular pressure) was lower in the hearts from overfed rats, and was reduced by I/R in hearts from control but not from overfed rats. I/R also reduced the coronary vasoconstriction to angiotensin II more in hearts from control than from overfed rats, and the vasodilatation to bradykinin similarly in both experimental groups. The expression of both angiotensin AGTRa and AGTR2 receptors was increased in the myocardium of overfed rats, and I/R increased the expression of both receptors in control rats but reduced it in overfed rats. The expression of apoptotic and antiapoptotic markers was increased in hearts of overfed rats compared with control, and further increased by I/R.
  
  
  CONCLUSIONS
  These results suggest that both overfeeding and I/R impair cardiac and coronary function due, at least in part, to activation of the angiotensin pathway. However, overfeeding may reduce the impairment of ventricular contractility by I/R.
authors: Department of Physiology, Faculty of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.
authors: Miriam
authors: M
authors: Granado
authors: Nuria
authors: N
authors: Fernández
authors: Luis
authors: L
authors: Monge
authors: Juan Carlos
authors: JC
authors: Figueras
authors: Gonzalo
authors: G
authors: Carreño-Tarragona
authors: Sara
authors: S
authors: Amor
authors: Angel Luis
authors: AL
authors: García-Villalón
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000833:Animals, Suckling; D015151:Immunoblotting; D008098:Litter Size; D009200:Myocardial Contraction / Q000502:physiology*; D015425:Myocardial Reperfusion / Q000009:adverse effects*; D044343:Overnutrition / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D011945:Receptors, Angiotensin / Q000378:metabolism*; D014664:Vasodilation / Q000502:physiology*
chemical_list: D011945:Receptors, Angiotensin
references: Circulation. 1996 Dec 15;94(12):3087-9
references: 8989113
references: Metabolism. 2012 Jun;61(6):812-22
references: 22209665
references: Cardiovasc Res. 2004 Jun 1;62(3):460-7
references: 15158138
references: Nat Clin Pract Endocrinol Metab. 2007 Dec;3(12):810-8
references: 18026159
references: J Hypertens. 2001 Sep;19(9):1681-9
references: 11564990
references: Curr Hypertens Rep. 2008 Apr;10(2):93-8
references: 18474174
references: Diabetologia. 2011 Apr;54(4):722-30
references: 21085926
references: Basic Res Cardiol. 2005 Jul;100(4):346-54
references: 15821998
references: Am J Physiol Endocrinol Metab. 2002 Jan;282(1):E59-66
references: 11739084
references: Neuroreport. 2007 Mar 26;18(5):521-4
references: 17496815
references: Am J Physiol Renal Physiol. 2011 Mar;300(3):F700-6
references: 21209001
references: Biochim Biophys Acta. 2010 Mar;1801(3):311-9
references: 19818871
references: Biotechniques. 1993 Sep;15(3):532-4, 536-7
references: 7692896
references: Nutr Metab Cardiovasc Dis. 2012 Mar;22(3):269-76
references: 21208789
references: Cardiovasc Res. 2009 Feb 1;81(2):336-43
references: 19029135
references: Endocrinology. 2010 Apr;151(4):1598-610
references: 20194730
references: Cytokine. 2011 Dec;56(3):688-94
references: 21975128
references: Am J Med Sci. 2001 Apr;321(4):225-36
references: 11307864
references: Nutr Metab Cardiovasc Dis. 2008 Nov;18(9):613-7
references: 18083356
references: Obes Facts. 2012;5(1):138-50
references: 22433625
references: Histol Histopathol. 2006 May;21(5):459-66
references: 16493576
references: Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1206-15
references: 11893553
references: Int J Obes (Lond). 2007 Feb;31(2):371-7
references: 16801924
references: Lancet. 2007 Apr 7;369(9568):1208-19
references: 17416265
references: Trends Pharmacol Sci. 2008 Jul;29(7):367-74
references: 18579222
references: Int J Obes Relat Metab Disord. 2000 Jun;24 Suppl 2:S142-3
references: 10997636
references: Clin Physiol Funct Imaging. 2006 Jul;26(4):197-204
references: 16836691
references: Diabetes. 2005 Jan;54(1):197-203
references: 15616029
references: Am J Cardiol. 2009 Oct 15;104(8):1116-21
references: 19801034
references: Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1784-9
references: 10677535
references: Clin Exp Pharmacol Physiol. 2008 Nov;35(11):1358-64
references: 18759854
references: Diabetes. 2008 Mar;57(3):669-77
references: 18057089
references: J Hypertens. 2002 May;20(5):965-73
references: 12011658
references: Clin Pharmacol Ther. 2011 Jul;90(1):174-8
references: 21562487
references: Int J Obes Relat Metab Disord. 2000 Jun;24(6):673-8
references: 10878672
references: Eur J Pharmacol. 2005 Feb 21;509(2-3):165-70
references: 15733552
references: J Am Coll Cardiol. 2004 May 5;43(9):1590-5
references: 15120816
references: Neurosci Lett. 2000 Oct 20;293(1):21-4
references: 11065128
references: Am J Physiol Endocrinol Metab. 2005 Jun;288(6):E1236-43
references: 15644456
references: Proc Nutr Soc. 2011 May;70(2):268-75
references: 21232171
references: Pediatr Cardiol. 2011 Jun;32(5):659-66
references: 21431306
references: Am J Clin Nutr. 1994 May;59(5):955-9
references: 8172099
references: N Engl J Med. 2002 Aug 1;347(5):305-13
references: 12151467
references: N Engl J Med. 2007 Dec 6;357(23):2329-37
references: 18057335
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Am J Physiol Regul Integr Comp Physiol. 2004 Oct;287(4):R943-9
references: 15191907
references: J Nutr. 2008 Sep;138(9):1622-7
references: 18716160
references: Brain Res. 1999 Jul 31;836(1-2):146-55
references: 10415413
references: Circulation. 2002 Jul 2;106(1):106-11
references: 12093778
references: Curr Pharm Des. 2009;15(10):1063-71
references: 19355948
references: Endocrinology. 2007 Jan;148(1):324-31
references: 17038553
references: Circulation. 2005 Apr 19;111(15):1962-9
references: 15837950
references: J Mol Cell Cardiol. 1996 Aug;28(8):1737-46
references: 8877783
references: Hypertension. 2010 Jan;55(1):76-82
references: 19901159
references: Regul Pept. 2000 Jun 30;90(1-3):19-25
references: 10828488
references: Am J Physiol Heart Circ Physiol. 2003 Nov;285(5):H2179-87
references: 12869376
references: Circulation. 2004 May 11;109(18):2191-6
references: 15123530
references: Apoptosis. 2012 Aug;17(8):908-15
references: 22527949
references: Int J Biochem Cell Biol. 2003 Jun;35(6):826-37
references: 12676169