title: Genetic variation of FASN is associated with fatty acid composition of Hanwoo.
abstract: The objective of this study was to identify genetic variants in the bovine fatty acid synthase (FASN) gene and to evaluate associations with fatty acid composition from longissimus lumborum muscle using 90 purebred Hanwoo steers. Sequence alignments observed 6 genetic variants located in exons 20, 24, 32, 34, and 39, and PCR-RFLP analysis confirmed these variations. Genotypes of the g.15532A>C locus were significantly associated with Linolenic acid (C18:3), and genotypes of the g.17924G>A locus were significantly associated with Palmitic (C16:0), Palmitoleic (C16:1), Oleic (C18:1), saturated fatty acids, and unsaturated fatty acids. The analysis revealed that SFA and UFA showed significant correlations with fatty acid composition (Myristic (C14:0), Palmitic (C16:0), Stearic (C18:0), Oleic (C18:1), and Eicosenoic (C20:1) acids). Oleic acid (C18:1) was negatively correlated with Myristic (C14:0), Palmitic (C16:0), and Palmitoleic (C16:1) acids (P<0.001).
authors: National Institute of Animal Science, Pyeongchang, Republic of Korea.
authors: S H
authors: SH
authors: Yeon
authors: S H
authors: SH
authors: Lee
authors: B H
authors: BH
authors: Choi
authors: H J
authors: HJ
authors: Lee
authors: G W
authors: GW
authors: Jang
authors: K T
authors: KT
authors: Lee
authors: K H
authors: KH
authors: Kim
authors: J H
authors: JH
authors: Lee
authors: H Y
authors: HY
authors: Chung
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics / Q000378:metabolism; D004032:Diet; D004041:Dietary Fats / Q000032:analysis*; D005091:Exons; D064429:Fatty Acid Synthases / Q000235:genetics*; D005227:Fatty Acids / Q000235:genetics* / Q000378:metabolism; D056426:Genetic Loci; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008460:Meat; D018482:Muscle, Skeletal / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D064429:Fatty Acid Synthases