title: Bacterial genomes in epidemiology--present and future.
abstract: Sequence data are well established in the reconstruction of the phylogenetic and demographic scenarios that have given rise to outbreaks of viral pathogens. The application of similar methods to bacteria has been hindered in the main by the lack of high-resolution nucleotide sequence data from quality samples. Developing and already available genomic methods have greatly increased the amount of data that can be used to characterize an isolate and its relationship to others. However, differences in sequencing platforms and data analysis mean that these enhanced data come with a cost in terms of portability: results from one laboratory may not be directly comparable with those from another. Moreover, genomic data for many bacteria bear the mark of a history including extensive recombination, which has the potential to greatly confound phylogenetic and coalescent analyses. Here, we discuss the exacting requirements of genomic epidemiology, and means by which the distorting signal of recombination can be minimized to permit the leverage of growing datasets of genomic data from bacterial pathogens.
authors: Department of Epidemiology, Center for Communicable Disease Dynamics, Harvard School of Public Health, Boston, MA, USA.
authors: Nicholas J
authors: NJ
authors: Croucher
authors: Simon R
authors: SR
authors: Harris
authors: Yonatan H
authors: YH
authors: Grad
authors: William P
authors: WP
authors: Hanage
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001424:Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D022761:Gene Transfer, Horizontal / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D008957:Models, Genetic; D017720:Molecular Epidemiology / Q000379:methods* / Q000639:trends
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