title: Chitosan-hyaluronan/nano chondroitin sulfate ternary composite sponges for medical use.
abstract: In this work chitosan-hyaluronan composite sponge incorporated with chondroitin sulfate nanoparticle (nCS) was developed. The fabrication of hydrogel was based on simple ionic cross-linking using EDC, followed by lyophilization to obtain the composite sponge. nCS suspension was characterized using DLS and SEM and showed a size range of 100-150 nm. The composite sponges were characterized using SEM, FT-IR and TG-DTA. Porosity, swelling, biodegradation, blood clotting and platelet activation of the prepared sponges were also evaluated. Nanocomposites showed a porosity of 67% and showed enhanced swelling and blood clotting ability. Cytocompatibility and cell adhesion studies of the sponges were done using human dermal fibroblast (HDF) cells and the nanocomposite sponges showed more than 90% viability. Nanocomposite sponges also showed enhanced proliferation of HDF cells within two days of study. These results indicated that this nanocomposite sponges would be a potential candidate for wound dressing.
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi-682 041, India.
authors: B S
authors: BS
authors: Anisha
authors: Deepthi
authors: D
authors: Sankar
authors: Annapoorna
authors: A
authors: Mohandas
authors: K P
authors: KP
authors: Chennazhi
authors: Shantikumar V
authors: SV
authors: Nair
authors: R
authors: R
authors: Jayakumar
mesh_terms: D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D015539:Platelet Activation / Q000187:drug effects; D016062:Porosity; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D014867:Water; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D048271:Chitosan