title: The use of one step nucleic-acid amplification (OSNA) in clinical practice: a single-centre study.
abstract: BACKGROUND
  The development of intraoperative sentinel node biopsy (SLNB) analysis methods, such as One-Step Nucleic Acid Amplification (OSNA), has made single-stage procedures possible.
  
  
  OBJECTIVE
  We investigated the incidence of OSNA-detected lymph node positivity, comparing it to conventional histopathology, the incidence of non-SLNB node disease for the OSNA positive patients, and the breast re-operation rate, to assess the benefit from single-stage procedures.
  
  
  METHODS
  This was a single-centre series of 573 consecutive patients undergoing SLNB (173 histopathology and 400 OSNA).
  
  
  RESULTS
  OSNA-detected SLNB macrometastasis was similar to routine histopathology, with more micrometastasis detected (p < 0.001). Non-SLNB involvement in the OSNA group was similar to documented histopathological series. 27.6% of OSNA patients avoided further surgery because of OSNA. The median time for OSNA results was 42 min.
  
  
  CONCLUSIONS
  OSNA is an effective method for detecting SLNB-metastasis. It is easily used in clinical practice, providing reliable results and negating the need for a second axillary operation.
authors: Department of Breast Surgery, Wycombe Hospital, Buckinghamshire Healthcare NHS Trust, Queen Alexandra Road, High Wycombe HP11 2TT, UK.
authors: Dionysios D
authors: DD
authors: Remoundos
authors: Department of Breast Surgery, Wycombe Hospital, Buckinghamshire Healthcare NHS Trust, Queen Alexandra Road, High Wycombe HP11 2TT, UK.
authors: Vivien V
authors: VV
authors: Ng
authors: Department of Breast Surgery, Wycombe Hospital, Buckinghamshire Healthcare NHS Trust, Queen Alexandra Road, High Wycombe HP11 2TT, UK.
authors: Hannah A
authors: HA
authors: Wilson
authors: Department of Breast Surgery, Wycombe Hospital, Buckinghamshire Healthcare NHS Trust, Queen Alexandra Road, High Wycombe HP11 2TT, UK.
authors: Farid
authors: F
authors: Ahmed
authors: Department of Pathology, Wycombe Hospital, Buckinghamshire Healthcare NHS Trust, Queen Alexandra Road, High Wycombe HP11 2TT, UK.
authors: Yoon
authors: Y
authors: Chia
authors: Department of Breast Surgery, Wycombe Hospital, Buckinghamshire Healthcare NHS Trust, Queen Alexandra Road, High Wycombe HP11 2TT, UK. Electronic address: gcunnick@doctors.org.uk.
authors: Giles H
authors: GH
authors: Cunnick
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000473:pathology / Q000601:surgery; D018567:Breast Neoplasms, Male / Q000601:surgery; D005260:Female; D006801:Humans; D007432:Intraoperative Period; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012086:Reoperation / Q000706:statistics & numerical data; D021701:Sentinel Lymph Node Biopsy