title: [Expression changes of BDNF in lung of rats subjected to brain ischemia].
abstract: OBJECTIVE
  To investigate the expression of brain-derived neurotrophic factor (BDNF) in lung injury induced by brain ischemia in rats.
  
  
  METHODS
  46 adult SD rats were assigned randomly to sham operation group and brain ischemia lung injury group (BILI, n = 23 in each group). Rats were subjected brain ischemia and allowed to survived 3 d. After performed neurological functional severe deficit evaluation, lung edema was observed (n=5). The BDNF expression for its mRNA and protein in lung tissues was determined by using ELISA (n=5) and RT-PCR technique (n=8). The localization of BDNF was also determined by immunohistochemistry (n=5).
  
  
  RESULTS
  After brain ischemia for 3 days, the severe neurological functional deficit and edema in lung were seen. BDNF was located in cytoplasma of smooth muscle and epithial cells in the lung. The level of BDNF mRNA (indicated by RT-PCR) and the protein level (indicated by ELISA) were all upregulated at 3 days after brain ischemia (P<0.05).
  
  
  CONCLUSIONS
  Lung edema occurred after brain ischemia in rats is concomitant with BDNF expression, which consists of the mechanism involved lung injury induced by brain ischemia.
authors: Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Qi-Qin
authors: QQ
authors: Dan
authors: Ping
authors: P
authors: Dai
authors: Sheng-Lan
authors: SL
authors: Wang
authors: Jin-Tao
authors: JT
authors: Li
authors: Yun-Hui
authors: YH
authors: Zhang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008168:Lung / Q000378:metabolism*; D011654:Pulmonary Edema / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger