title: Noncanonical EF-hand motif strategically delays Ca2+ buffering to enhance cardiac performance.
abstract: EF-hand proteins are ubiquitous in cell signaling. Parvalbumin (Parv), the archetypal EF-hand protein, is a high-affinity Ca(2+) buffer in many biological systems. Given the centrality of Ca(2+) signaling in health and disease, EF-hand motifs designed to have new biological activities may have widespread utility. Here, an EF-hand motif substitution that had been presumed to destroy EF-hand function, that of glutamine for glutamate at position 12 of the second cation binding loop domain of Parv (ParvE101Q), markedly inverted relative cation affinities: Mg(2+) affinity increased, whereas Ca(2+) affinity decreased, forming a new ultra-delayed Ca(2+) buffer with favorable properties for promoting cardiac relaxation. In therapeutic testing, expression of ParvE101Q fully reversed the severe myocyte intrinsic contractile defect inherent to expression of native Parv and corrected abnormal myocardial relaxation in diastolic dysfunction disease models in vitro and in vivo. Strategic design of new EF-hand motif domains to modulate intracellular Ca(2+) signaling could benefit many biological systems with abnormal Ca(2+) handling, including the diseased heart.
authors: Department of Integrative Biology and Physiology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
authors: Wang
authors: W
authors: Wang
authors: Matthew S
authors: MS
authors: Barnabei
authors: Michelle L
authors: ML
authors: Asp
authors: Frazer I
authors: FI
authors: Heinis
authors: Erik
authors: E
authors: Arden
authors: Jennifer
authors: J
authors: Davis
authors: Elizabeth
authors: E
authors: Braunlin
authors: Qi
authors: Q
authors: Li
authors: Jonathan P
authors: JP
authors: Davis
authors: James D
authors: JD
authors: Potter
authors: Joseph M
authors: JM
authors: Metzger
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D002147:Calmodulin / Q000737:chemistry / Q000378:metabolism; D020832:EF Hand Motifs; D005260:Female; D006321:Heart / Q000502:physiology; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009119:Muscle Contraction; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000502:physiology*; D010320:Parvalbumins / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment
chemical_list: D002135:Calcium-Binding Proteins; D002147:Calmodulin; D010320:Parvalbumins; D008274:Magnesium; D002118:Calcium
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