title: ERK signaling is triggered by hepatitis C virus E2 protein through DC-SIGN.
abstract: Dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) is a binding receptor for hepatitis C virus (HCV). Binding of HCV envelope protein E2 to target cells is a prerequisite to DC-SIGN-mediated signaling. Using cell lines with stable or transient expression of DC-SIGN, we investigated effects of soluble HCV E2 protein on ERK pathway. MEK and ERK are activated by the E2 in NIH3T3 cells stably expressing DC-SIGN. Treatment of the cells with antibody to DC-SIGN results in inhibition of the E2 binding as well as the E2-induced MEK and ERK activation. In HEK293T cells transiently expressing DC-SIGN, activation of MEK and ERK is also induced by the E2. Activation of ERK pathway by HCV E2 through DC-SIGN provides useful information for understanding cellular receptor-mediated signaling.
authors: Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China.
authors: Lan-Juan
authors: LJ
authors: Zhao
authors: Wen
authors: W
authors: Wang
authors: Hao
authors: H
authors: Ren
authors: Zhong-Tian
authors: ZT
authors: Qi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D057809:HEK293 Cells; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D041681:NIH 3T3 Cells; D011485:Protein Binding / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism / Q000494:pharmacology*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000906:Antibodies; D015815:Cell Adhesion Molecules; D048049:Extracellular Signal-Regulated MAP Kinases; D037181:Lectins, C-Type; D020930:MAP Kinase Kinase Kinases; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C091015:glycoprotein E2, Hepatitis C virus
references: J Gen Virol. 2011 Oct;92(Pt 10):2237-2248
references: 21653755
references: Cell. 2000 Mar 3;100(5):575-85
references: 10721994
references: J Biol Chem. 2003 May 30;278(22):20358-66
references: 12609975
references: Future Microbiol. 2009 Sep;4(7):879-90
references: 19722841
references: J Virol. 2012 Dec;86(23):12531-43
references: 22951829
references: J Biol Chem. 2003 Sep 12;278(37):35755-66
references: 12824191
references: Cell Prolif. 2007 Aug;40(4):508-21
references: 17635518
references: Methods Enzymol. 2010;480:151-64
references: 20816209
references: J Infect Dis. 2003 Oct 15;188(8):1192-204
references: 14551890
references: Immunity. 2007 May;26(5):605-16
references: 17462920
references: Clin Infect Dis. 2005 Jul 1;41 Suppl 1:S32-7
references: 16265611
references: J Immunol. 2006 Feb 1;176(3):1741-9
references: 16424204
references: J Virol. 2003 Apr;77(7):4070-80
references: 12634366
references: Methods Mol Biol. 2007;379:51-68
references: 17502670
references: EMBO J. 2002 Oct 1;21(19):5017-25
references: 12356718
references: Immunity. 2002 Nov;17(5):653-64
references: 12433371
references: Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4498-503
references: 12676990
references: Nat Immunol. 2010 May;11(5):419-26
references: 20364151
references: J Virol. 1999 Aug;73(8):6235-44
references: 10400713
references: J Biol Chem. 2005 Mar 25;280(12):11329-39
references: 15611113
references: J Virol. 2008 Sep;82(17):8316-29
references: 18579606
references: Oncogene. 2001 May 3;20(20):2606-10
references: 11420671
references: Cell Signal. 2010 Oct;22(10):1397-405
references: 20363321
references: Biochem Biophys Res Commun. 2008 Sep 5;373(4):561-6
references: 18593570
references: J Neurovirol. 2011 Aug;17(4):327-40
references: 21660601
references: J Virol. 2006 Apr;80(7):3477-86
references: 16537615
references: Cancer Immunol Immunother. 2009 Jul;58(7):1149-57
references: 18998127
references: Exp Cell Res. 2005 Apr 15;305(1):23-32
references: 15777784
references: J Gen Virol. 2008 Oct;89(Pt 10):2398-2409
references: 18796707
references: J Exp Med. 2003 Apr 7;197(7):823-9
references: 12682107
references: J Virol. 2001 May;75(10):4664-72
references: 11312337
references: J Virol. 2006 Feb;80(3):1290-301
references: 16415006
references: Nat Immunol. 2007 Jun;8(6):569-77
references: 17496896
references: Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14067-72
references: 15371595
references: J Virol. 2000 Nov;74(21):10055-62
references: 11024134
references: J Virol. 2002 Jun;76(12):5905-14
references: 12021323
references: Virology. 2004 Jun 20;324(1):74-83
references: 15183055
references: J Pathol. 2002 Dec;198(4):511-6
references: 12434421
references: Blood. 2006 May 15;107(10):3950-8
references: 16434485
references: Annu Rev Immunol. 2007;25:443-72
references: 17243893
references: J Virol. 2012 Feb;86(3):1544-54
references: 22114332
references: J Virol. 2012 Feb;86(3):1339-47
references: 22090124
references: Cell Biochem Biophys. 2010;56(1):49-58
references: 19851890
references: J Leukoc Biol. 2006 Aug;80(2):424-32
references: 16793913
references: J Virol. 2002 Jul;76(13):6841-4
references: 12050398
references: Biochem Biophys Res Commun. 2008 Dec 12;377(2):367-372
references: 18848918