title: Transmission of human enterovirus 85 recombinants containing new unknown serotype HEV-B donor sequences in Xinjiang Uighur autonomous region, China.
abstract: BACKGROUND
  Human enterovirus 85 (HEV85), whose prototype strain (Strain BAN00-10353/BAN/2000) was isolated in Bangladesh in 2000, is a recently identified serotype within the human enterovirus B (HEV-B) species. At present, only one nucleotide sequence of HEV85 (the complete genome sequence of the prototype strain) is available in the GenBank database.
  
  
  RESULTS
  In this study, we report the genetic characteristics of 33 HEV85 isolates that circulated in the Xinjiang Uighur autonomous region of China in 2011. Sequence analysis revealed that all these Chinese HEV85 isolates belong to 2 transmission chains, and intertypic recombination was found with the new unknown serotype HEV-B donor sequences. Two HEV85 isolates recovered from a patient presenting acute flaccid paralysis and one of his contacts were temperature-insensitive strains, and some nucleotide substitutions in the non-coding regions and in the 2C or 3D coding regions may have affected the temperature sensitivity of HEV85 strains.
  
  
  CONCLUSIONS
  The Chinese HEV85 recombinant described in this study trapped a new unknown serotype HEV-B donor sequence, indicating that new unknown HEV-B serotypes exist or circulate in Xinjiang of China. Our study also indicated that HEV85 is a prevalent and common enterovirus serotype in Xinjiang.
authors: WHO WPRO Regional Polio Reference Laboratory, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
authors: Qiang
authors: Q
authors: Sun
authors: Yong
authors: Y
authors: Zhang
authors: Shuangli
authors: S
authors: Zhu
authors: Huifang
authors: H
authors: Tian
authors: Guohong
authors: G
authors: Huang
authors: Hui
authors: H
authors: Cui
authors: Xiaolei
authors: X
authors: Li
authors: Dongmei
authors: D
authors: Yan
authors: Zhen
authors: Z
authors: Zhu
authors: Jing
authors: J
authors: Li
authors: Peng
authors: P
authors: Zheng
authors: Huafang
authors: H
authors: Jiang
authors: Bo
authors: B
authors: Zhang
authors: Xiaojuan
authors: X
authors: Tan
authors: Hui
authors: H
authors: Zhu
authors: Hongqiu
authors: H
authors: An
authors: Wenbo
authors: W
authors: Xu
mesh_terms: D002681:China / Q000453:epidemiology; D019295:Computational Biology; D029822:Enterovirus B, Human / Q000145:classification* / Q000235:genetics*; D004769:Enterovirus Infections / Q000453:epidemiology / Q000635:transmission*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013696:Temperature
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