title: Changes in γ-H2AX expression in irradiated feline sarcoma cells: an indicator of double strand DNA breaks.
abstract: Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.
authors: Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. lawrence.uga@gmail.com
authors: J
authors: J
authors: Lawrence
authors: E
authors: E
authors: Karpuzoglu
authors: A
authors: A
authors: Vance
authors: M
authors: M
authors: Vandenplas
authors: C
authors: C
authors: Saba
authors: M
authors: M
authors: Turek
authors: R M
authors: RM
authors: Gogal
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002371:Cat Diseases / Q000378:metabolism / Q000532:radiotherapy*; D002415:Cats; D045744:Cell Line, Tumor; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects*; D006657:Histones / Q000096:biosynthesis / Q000276:immunology / Q000528:radiation effects*; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012509:Sarcoma / Q000378:metabolism / Q000532:radiotherapy / Q000662:veterinary*
chemical_list: D000906:Antibodies; D006657:Histones; D011933:Reagent Kits, Diagnostic