title: Recent development of phosphorylases possessing large potential for oligosaccharide synthesis.
abstract: Phosphorylases are one group of carbohydrate active enzymes involved in the cleavage and formation of glycosidic linkages together with glycoside hydrolases and sugar nucleotide-dependent glycosyltransferases. Noticeably, the catalyzed phosphorolysis is reversible, making phosphorylases suitable catalysts for efficient synthesis of particular oligosaccharides from a donor sugar 1-phosphate and suitable carbohydrate acceptors with strict regioselectivity. Although utilization of phosphorylases for oligosaccharide synthesis has been limited because only few different enzymes are known, recently the number of reported phosphorylases has gradually increased, providing the variation making these enzymes useful tools for efficient synthesis of diverse oligosaccharides.
authors: Graduate School of Science & Technology, Niigata University, 8050, Ikarashi 2-no-cho, Nishi-ku, Niigata 950-2181, Japan. nakai@agr.niigata-u.ac.jp
authors: Hiroyuki
authors: H
authors: Nakai
authors: Motomitsu
authors: M
authors: Kitaoka
authors: Birte
authors: B
authors: Svensson
authors: Ken'ichi
authors: K
authors: Ohtsubo
mesh_terms: D000818:Animals; D001671:Biochemistry; D001709:Biotechnology; D006801:Humans; D009844:Oligosaccharides / Q000096:biosynthesis*; D006005:Phosphorylases / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D006005:Phosphorylases