title: The wide-field optical sectioning of microlens array and structured illumination-based plane-projection multiphoton microscopy.
abstract: We present a theoretical investigation of an optical microscope design that achieves wide-field, multiphoton fluorescence microscopy with finer axial resolution than confocal microscopy. Our technique creates a thin plane of excitation light at the sample using height-staggered microlens arrays (HSMAs), wherein the height staggering of microlenses generate temporal focusing to suppress out-of-focus excitation, and the dense spacing of microlenses enables the implementation of structured illumination technique to eliminate residual out-of-focus signal. We use physical optics-based numerical simulations to demonstrate that our proposed technique can achieve diffraction-limited three-dimensional imaging through a simple optical design.
authors: Bioengineering, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, California 91125, USA.
authors: Jiun-Yann
authors: JY
authors: Yu
authors: Daniel B
authors: DB
authors: Holland
authors: Geoffrey A
authors: GA
authors: Blake
authors: Chin-Lin
authors: CL
authors: Guo
mesh_terms: D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods*; D007909:Lenses; D008029:Lighting / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D008904:Miniaturization; D008962:Models, Theoretical