title: Chordin-like protein 1 promotes neuronal differentiation by inhibiting bone morphogenetic protein-4 in neural stem cells.
abstract: In the present study, the effects of chordin‑like protein 1 (CHRDL1) overexpression, together with bone morphogenetic protein‑4 (BMP‑4) treatment, on the differentiation of rat spinal cord‑derived neural stem cells (NSCs) was investigated. Adult rat spinal cord‑derived NSCs were cultured in serum‑free medium. The recombined eukaryotic expression vector pSecTag2/Hygro B‑CHRDL1 was transfected into adult rat spinal cord‑derived NSCs using a lipid‑based transfection reagent and protein expression was assessed by western blot analysis. Differentiation of transfected NSCs following BMP‑4 treatment was determined by immunocytochemistry. The percentage of microtubule‑associated protein‑2 (MAP‑2)‑positive cells in the BMP‑4‑treated (B) group was found to be significantly lower compared with that in the non‑transfected control (N) group. The percentage of MAP‑2‑positive cells in the pSecTag2/Hygro B‑CHRDL1‑transfected, BMP‑4‑treated group was identified to be significantly higher compared with that in group B, however, no significant difference was observed between group N and the transfected, non‑BMP‑4‑treated control group. The current study indicates that CHRDL1 protein antagonizes BMP‑4 activity and induces spinal cord‑derived NSCs to differentiate into neurons.
authors: Department of Orthopaedics, The Geriatric Institution, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.
authors: Wei-Lu
authors: WL
authors: Gao
authors: Sheng-Quan
authors: SQ
authors: Zhang
authors: Hui
authors: H
authors: Zhang
authors: Bo
authors: B
authors: Wan
authors: Zong-Sheng
authors: ZS
authors: Yin
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D005136:Eye Proteins / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism
chemical_list: C525809:BMP4 protein, human; C525811:Bmp4 protein, rat; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; C486602:CHRDL1 protein, human; C480495:CHRDL1 protein, rat; D005136:Eye Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C054234:chordin