title: Beta globin frameworks in thalassemia major patients from north iran.
abstract: OBJECTIVE
  Four combinations of five neutral sequence changes at rs713040, rs10768683, rs7480526, rs7946748, and rs1609812 occurring in the human beta globin gene defined as frameworks have been reported in beta globin gene. Here we report for the frequency of these frameworks in thalassemia major patients of North Iran.
  
  
  METHODS
  Beta globin gene frameworks of 46 thalassemia major patients of North Iran were determined using Denaturing Gradient Gel Electrophoresis.
  
  
  RESULTS
  All these frameworks called framework 1, 2, 3, 3a were present at the frequency of 23.9%, 45.7%, 6.5% and 23.9% respectively.
  
  
  CONCLUSIONS
  These frameworks may be used for tracking mutant alleles in prenatal diagnosis programs.
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran ; Genetic Laboratory, Amirkola Children's Hospital, Babol University of Medical Sciences, Babol, Iran.
authors: Haleh
authors: H
authors: Akhavan-Niaki
authors: Ali
authors: A
authors: Banihashemi
authors: Mandana
authors: M
authors: Azizi
keywords: Beta–Globin; Denaturing Gradient Gel Electrophoresis; Polymorphism; Thalassemia
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