title: Thermospermine catabolism increases Arabidopsis thaliana resistance to Pseudomonas viridiflava.
abstract: This work investigated the roles of the tetraamine thermospermine (TSpm) by analysing its contribution to Arabidopsis basal defence against the biotrophic bacterium Pseudomonas viridiflava. The participation of polyamine oxidases (PAOs) in TSpm homeostasis and TSpm-mediated defence was also investigated. Exogenous supply of TSpm, as well as ectopic expression of the TSpm biosynthetic gene ACL5, increased Arabidopsis Col-0 resistance to P. viridiflava, while null acl5 mutants were less resistant than Col-0 plants. The above-mentioned increase in resistance was blocked by the PAO inhibitor SL-11061, thus demonstrating the participation of TSpm oxidation. Analysis of PAO genes expression in transgenic 35S::ACL5 and Col-0 plants supplied with TSpm suggests that PAO 1, 3, and 5 are the main PAOs involved in TSpm catabolism. In summary, TSpm exhibited the potential to perform defensive functions previously reported for its structural isomer Spm, and the relevance of these findings is discussed in the context of ACL5 expression and TSpm concentration in planta. Moreover, this work demonstrates that manipulation of TSpm metabolism modifies plant resistance to pathogens.
authors: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús, Universidad Nacional de General San Martín-Consejo Nacional de Investigaciones Científicas y Técnicas (IIB-INTECH/UNSAM-CONICET), Chascomús, Argentina. maria@intech.gov.ar
authors: María
authors: M
authors: Marina
authors: Francisco Vera
authors: FV
authors: Sirera
authors: José L
authors: JL
authors: Rambla
authors: María E
authors: ME
authors: Gonzalez
authors: Miguel A
authors: MA
authors: Blázquez
authors: Juan
authors: J
authors: Carbonell
authors: Fernando L
authors: FL
authors: Pieckenstain
authors: Oscar A
authors: OA
authors: Ruiz
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D060467:Disease Resistance / Q000187:drug effects / Q000276:immunology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D010084:Oxidation-Reduction / Q000187:drug effects; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D011700:Putrescine / Q000378:metabolism; D013096:Spermine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000097667:Polyamine Oxidase
chemical_list: D006631:Amine Oxidase (Copper-Containing); D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011700:Putrescine; D013096:Spermine; D000097667:Polyamine Oxidase; C021097:thermospermine