title: Determination of thalidomide concentration in human plasma by liquid chromatography-tandem mass spectrometry.
abstract: A rapid, sensitive and specific analytical method based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of thalidomide concentration in human plasma. The analyte and internal standard were extracted by liquid-liquid extraction with ether-dichloromethane (3:2, v/v) and separated on a TC-C(18) column using methanol-10 mM ammonium acetate-formic acid (60:40:0.04, v/v/v) as the mobile phase at a flow rate of 0.9 ml/min. The detection was performed using an API 4000 triple quadrupole mass spectrometer in the positive electrospray ionization (ESI) mode and completed within 3.0 min. The multiple reaction monitoring (MRM) transitions were m/z 259.1→84.0 for the analyte and m/z 195.9→138.9 for temozolomide. The calibration curve exhibited a linear dynamic range of 2-1500 ng/ml (r>0.9991). The intra-and inter-day precisions (as relative standard deviation; RSD) were 6.8-13.5% and 4.3-5.0% respectively and the accuracy (as relative error; RE) was 2.0-3.5%. The recoveries and matrix effects were satisfactory in all the biological matrices examined. This method was successfully used in a pharmacokinetic study of thalidomide in healthy male volunteers receiving an oral administration of a 200-mg dose.
authors: The Center of Medicine Clinical Research, Chinese PLA General Hospital, Beijing 100853;
authors: Nan
authors: N
authors: Bai
authors: Xiang-Yong
authors: XY
authors: Cui
authors: Jin
authors: J
authors: Wang
authors: Chun-Guang
authors: CG
authors: Sun
authors: He-Kun
authors: HK
authors: Mei
authors: Bei-Bei
authors: BB
authors: Liang
authors: Yun
authors: Y
authors: Cai
authors: Xiu-Jie
authors: XJ
authors: Song
authors: Jing-Kai
authors: JK
authors: Gu
authors: Rui
authors: R
authors: Wang
keywords: human plasma; liquid chromatography-tandem mass spectrometry; multiple reaction monitoring; thalidomide
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