title: Virus-induced gene silencing using artificial miRNAs in Nicotiana benthamiana.
abstract: Virus-induced gene silencing using artificial microRNAs (MIR VIGS) is a newly developed technique for plant reverse genetic studies. Traditional virus-induced gene silencing (VIGS) assays introduce a large gene fragment, which is expressed and then converted into small RNAs by the endogenous siRNA-based gene silencing machinery of the plant host. By contrast, MIR VIGS uses well-designed miRNAs to induce RNA-mediated silencing of the target gene. Using a single artificial miRNA can provide greater specificity by reducing off-target effects. Here, we describe a detailed protocol for MIR VIGS in Nicotiana benthamiana using a modified Cabbage leaf curl virus (CaLCuV)-based vector.
authors: School of Life Sciences, Tsinghua University, Beijing, China.
authors: Yang
authors: Y
authors: Tang
authors: Yizhen
authors: Y
authors: Lai
authors: Yule
authors: Y
authors: Liu
mesh_terms: D016960:Agrobacterium tumefaciens / Q000821:virology; D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics; D003001:Cloning, Molecular; D055785:Gene Knockdown Techniques / Q000379:methods*; D017343:Genes, Plant; D005822:Genetic Vectors; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014169:Transformation, Bacterial
chemical_list: D035683:MicroRNAs