title: Fluorophotometric determination of histone with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone-manganese(II) complex and its characterization.
abstract: A simple fluorophotometric method for the determination of histone has been developed. This method involves a fluorescence quenching reaction that results in the formation of a complex of manganese(II), 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone (TFCPF), and histone in a non-ionic surfactant micellar medium. The calibration curve was found to be linear in the range of 0.5 to 2.0 µg/mL. The binding parameters (n, number of binding sites; K, binding constant) and thermodynamic parameters (ΔG(0), change in Gibbs free energy; ΔH(0), change in enthalpy; ΔS(0), change in entropy) were investigated spectrophotometrically for the elucidation of the reaction mechanism. The resulting binding parameters (n=4.08 and K=3.16×10(4) m(-1) at 25°C) and thermodynamic parameters (ΔG=-25.83 kJ/mol, ΔH=-9.83 kJ/mol, and ΔS=53.68 J/(mol K)) suggest that the colored complex in this reaction system is an ion-association complex between manganese(II)-TFCPF and histone.
authors: Department of Hospital Pharmacy School of Medicine, Kinki University, Ohno-Higashi, Osaka-Sayama, Osaka, Japan.
authors: Kanako
authors: K
authors: Miyachi
authors: Mitsuru
authors: M
authors: Hoshino
authors: Hiroko
authors: H
authors: Kadobayashi
authors: Kenzo
authors: K
authors: Moriyama
authors: Mamiko
authors: M
authors: Asano
authors: Takako
authors: T
authors: Yamaguchi
authors: Yoshikazu
authors: Y
authors: Fujita
mesh_terms: D001665:Binding Sites; D002138:Calibration; D056831:Coordination Complexes / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D015590:Fluorophotometry / Q000592:standards; D006657:Histones / Q000032:analysis* / Q000592:standards; D007700:Kinetics; D008345:Manganese / Q000737:chemistry*; D008823:Micelles; D011485:Protein Binding; D013816:Thermodynamics
chemical_list: D056831:Coordination Complexes; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006657:Histones; D008823:Micelles; D008345:Manganese; C010159:fluorone Black