title: 14-3-3 σ is a new target up-regulated by transforming growth factor-β1 through a Smad3-dependent mechanism.
abstract: The seven members of the human 14-3-3 family play crucial roles in a diverse range of cellular responses including cell cycle progression, DNA damage checkpoint, and apoptosis. One particular isoform, 14-3-3 σ, the p53 target gene, is a unique tumor suppressor. We here report 14-3-3 σ as a transforming growth factor-beta (TGF-β) target gene. In mammary epithelial cells, TGF-β selectively induced expression of 14-3-3 σ at both mRNA and protein levels, and this induction was dependent on Smad3 not on p53. In addition, blockade of non-canonical Smad-independent pathways, including MAP kinases and Rho GTPases, did not affect the TGF-β1-induced 14-3-3 σ expression. Our data provides the first evidence that 14-3-3 σ is a Smad3-dependent target gene of TGF-β1.
authors: Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors: Hye-Young
authors: HY
authors: Hong
authors: Woo-Kwang
authors: WK
authors: Jeon
authors: Seong-Jin
authors: SJ
authors: Kim
authors: Byung-Chul
authors: BC
authors: Kim
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D048948:14-3-3 Proteins; C499130:Sfn protein, mouse; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D053773:Transforming Growth Factor beta1; D016159:Tumor Suppressor Protein p53