title: Alpinate oxyphyllae fructus (Alpinia Oxyphylla Miq) extracts inhibit angiotensin-II induced cardiac apoptosis in H9c2 cardiomyoblast cells.
abstract: Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100 µg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, pro-apoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.
authors: Department of Chinese Medicine, China Medical University Hospital, Taichung, Taiwan.
authors: Yung-Ming
authors: YM
authors: Chang
authors: Chuan-Te
authors: CT
authors: Tsai
authors: Chiun-Chuang Roger
authors: CC
authors: Wang
authors: Yueh-Sheng
authors: YS
authors: Chen
authors: Yueh-Min
authors: YM
authors: Lin
authors: Chia-Hua
authors: CH
authors: Kuo
authors: Bor-Show
authors: BS
authors: Tzang
authors: Ray-Jade
authors: RJ
authors: Chen
authors: Fuu-Jen
authors: FJ
authors: Tsai
authors: Chih-Yang
authors: CY
authors: Huang
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001562:Benzimidazoles; D002232:Carbocyanines; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian; D005456:Fluorescent Dyes; D005638:Fruit / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D032386:Myoblasts, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D051025:bcl-Associated Death Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494821:Bad protein, rat; D001562:Benzimidazoles; D002232:Carbocyanines; D002316:Cardiotonic Agents; D005456:Fluorescent Dyes; D010936:Plant Extracts; D051025:bcl-Associated Death Protein; D000804:Angiotensin II; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; D045304:Cytochromes c; D053148:Caspase 3; D053453:Caspase 9