title: Generating parabiotic zebrafish embryos for cell migration and homing studies.
abstract: Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds.
authors: Institut Pasteur, Unité Macrophages et Développement de l'Immunité, Département de Biologie du Développement et Cellules Souches, Paris, France.
authors: Doris Lou
authors: DL
authors: Demy
authors: Zachary
authors: Z
authors: Ranta
authors: Jean-Michel
authors: JM
authors: Giorgi
authors: Magali
authors: M
authors: Gonzalez
authors: Philippe
authors: P
authors: Herbomel
authors: Karima
authors: K
authors: Kissa
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002465:Cell Movement / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods*; D004625:Embryo, Nonmammalian; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010227:Parabiosis / Q000379:methods*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000502:physiology
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