title: Serial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo.
abstract: Hematopoietic stem cells (HSCs) have the ability to self-renew and replenish the blood and immune system for the life span of an individual. An age-associated decline in HSC function is responsible for the decreased immune function and increased incidence of myeloid diseases and anemia in the elderly. The changes in HSC function are thought to occur as the result of an intrinsic defect in the self-renewal potential of HSCs as they age. In this chapter, we describe a bone marrow serial transplantation protocol designed to test the self-renewal capacity of HSCs in vivo.
authors: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, MA, USA.
authors: Charusheila
authors: C
authors: Ramkumar
authors: Rachel M
authors: RM
authors: Gerstein
authors: Hong
authors: H
authors: Zhang
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D051379:Mice