title: Neural responses to electrical stimulation on patterned silk films.
abstract: Peripheral nerve injury is a critical issue for patients with trauma. Following injury, incomplete axon regeneration or misguided axon innervation into tissue will result in loss of sensory and motor functions. The objective of this study was to examine axon outgrowth and axon alignment in response to surface patterning and electrical stimulation. To accomplish our objective, metal electrodes with dimensions of 1.5 mm × 4 cm, were sputter coated onto micropatterned silk protein films, with surface grooves 3.5 μm wide × 500 nm deep. P19 neurons were seeded on the patterned electronic silk films and stimulated at 120 mV, 1 kHz, for 45 min each day for 7 days. Responses were compared with neurons on flat electronic silk films, patterned silk films without stimulation, and flat silk films without stimulation. Significant alignment was found on the patterned film groups compared with the flat film groups. Axon outgrowth was greater (p < 0.05) on electronic films on days 5 and 7 compared with the unstimulated groups. In conclusion, electrical stimulation, at 120 mV, 1 kHz, for 45 min daily, in addition to surface patterning, of 3.5 μm wide × 500 nm deep grooves, offered control of nerve axon outgrowth and alignment.
authors: Department of Biomedical Engineering, Science & Technology Center, Tufts University, Medford, Massachusetts 02155, USA.
authors: Marie
authors: M
authors: Hronik-Tupaj
authors: Waseem Khan
authors: WK
authors: Raja
authors: Min
authors: M
authors: Tang-Schomer
authors: Fiorenzo G
authors: FG
authors: Omenetto
authors: David L
authors: DL
authors: Kaplan
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D004553:Electric Conductivity; D004599:Electric Stimulation Therapy; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008856:Microscopy, Fluorescence; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010525:Peripheral Nerves / Q000502:physiology; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D047011:Silk
keywords: biomaterial; electrical stimulation; neuron; silk; tissue engineering
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