title: In vivo effect confirmation of anti-androgenic compounds in sediment contact tests with Potamopyrgus antipodarum.
abstract: In order to investigate the sensitivity of Potamopyrgus antipodarum to anti-androgenic compounds, three spiked sediment tests were performed. The substances benzanthrone (7H-benz[de]anthracen-7-one), traseolide (ATII) and androstenone (5α-Androst-16-en-3-one) were previously identified in an effect-directed analysis study of the river Schijn in the north of Belgium. Although, in previous studies, all of the three compounds exhibited anti-androgenic activities in vitro, only the oxy-PAH benzanthrone had significant stimulating effects on the snails' reproduction. The reproduction of P. antipodarum was significantly stimulated, following a sigmoidal dose response curve, whereby an EC(50) of 10 ng/g dry sediment was calculated. Mortality was significantly increased at the highest concentration (69 ng/g dry sediment). The results indicate different relative potencies for the in vivo test with P. antipodarum and the in vitro anti-AR-CALUX assay, performed in a previous study. This highlights the importance of combined in vitro and in vivo assays for the effect assessment of field sediments.
authors: University of Antwerp, Ecosystem Management Research Group, Wilrijk, Belgium. claudia.schmitt@ua.ac.be
authors: Claudia
authors: C
authors: Schmitt
authors: Marja
authors: M
authors: LaMoree
authors: Pim
authors: P
authors: Leonards
authors: Jana M
authors: JM
authors: Weiss
authors: Eric
authors: E
authors: de Deckere
mesh_terms: D000728:Androgens / Q000633:toxicity*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000633:toxicity; D052244:Endocrine Disruptors / Q000633:toxicity; D004784:Environmental Monitoring; D049851:Gastropoda / Q000187:drug effects* / Q000502:physiology*; D019015:Geologic Sediments / Q000032:analysis; D007189:Indans / Q000633:toxicity; D012098:Reproduction / Q000187:drug effects
chemical_list: C110695:5-acetyl-1,1,2,6-tetramethyl-3-isopropylindane; D000728:Androgens; D001551:Benz(a)Anthracenes; D052244:Endocrine Disruptors; D007189:Indans; C012768:benzanthrone