title: Intense exercise training is not effective to restore the endothelial NO-dependent relaxation in STZ-diabetic rat aorta.
abstract: BACKGROUND
  The aim of this study was to examine the effects of intense physical training on vascular function in streptozotocin-diabetic rats. We focused on the endothelium-dependent relaxation (EDR) induced by acetylcholine (ACh) and stable ADP adenosine-5'- O - (2-thiodiphosphate) (ADPβS).
  
  
  METHODS
  Control or diabetic male Wistar rats (n=44) were randomly assigned to sedentary or trained groups. The training program consisted in a regular period of running on a treadmill during 8 weeks (10° incline and up to 25 m/min, 60 min/day). The reactivity of isolated thoracic aorta rings of healthy, diabetic and/or trained has been tested.
  
  
  RESULTS
  ACh and ADPβS-induced EDR were observed in phenylephrine (PE) pre-contracted vessels. As compared to sedentary control group, diabetic rats showed an increase in PE-induced contraction and a decrease in ACh and ADPβS-induced EDR (p<0.05). Moreover, there were no increase in ACh and ADPβS-induced EDR in diabetic rats. N-Nitro-L-Arginine Methyl Ester inhibited the nitric oxide synthase in diabetic and control rats, thereby resulting in a strong inhibition of the EDR induced by ACh and ADPβS (10-6 M).
  
  
  CONCLUSIONS
  Diabetes induced an endothelium dysfunction. Nevertheless, our intense physical training was not effective to restore the aorta endothelial function.
authors: Laboratory Movement Sport and Health Sciences, UFR APS University of Rennes 2, Avenue Charles Tillon, Rennes cedex, France. sami-zguira@hotmail.fr
authors: Mohamed Sami
authors: MS
authors: Zguira
authors: Sophie
authors: S
authors: Vincent
authors: Solène
authors: S
authors: Le Douairon Lahaye
authors: Ludivine
authors: L
authors: Malarde
authors: Zouhair
authors: Z
authors: Tabka
authors: Bernard
authors: B
authors: Saïag
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology* / Q000628:therapy; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000379:methods / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D009569:Nitric Oxide
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