title: Nonradioactive in vitro assays for histone deacetylases.
abstract: The effect of histone deacetylases (HDACs) on normal and aberrant gene expression has been studied widely, making these enzymes interesting targets for the treatment of cancer and other diseases. In this chapter, we present in vitro assays that are commonly used to detect HDAC activity that do not rely on radioactive substrates and are amenable for high-throughput testing in microtiter plates. The major focus is on in vitro screening, but we also provide protocols to monitor HDAC activity from cancer cells and peripheral white blood cells. We will discuss the advantages and drawbacks of the respective protocols and give general hints and suggestions that are valuable to obtain reliable and reproducible results.
authors: Institute of Pharmaceutical Sciences, University of Freiburg, Freiburg, Germany.
authors: Alexander-Thomas
authors: AT
authors: Hauser
authors: Julia M
authors: JM
authors: Gajer Née Wagner
authors: Manfred
authors: M
authors: Jung
mesh_terms: D000107:Acetylation; D001681:Biological Assay / Q000379:methods*; D045744:Cell Line, Tumor / Q000201:enzymology*; D005454:Fluorescence Polarization; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*
chemical_list: D006657:Histones; D006655:Histone Deacetylases