title: Disrupting Rb-Raf-1 interaction inhibits hair cell regeneration in zebrafish lateral line neuromasts.
abstract: Zebrafish neuromast is an ideal model for investigating hair cell (HC) death and regeneration following ototoxic insults. HC undergoes rapid and robust replacement in larval zebrafish after neomycin damage. However, the origin of new HCs remains unclear. Our data showed that asymmetric cell division was involved in the process of HC regeneration in zebrafish lateral line neuromasts. Furthermore, a small molecule RRD251, which disrupted the physical interaction between RB and Raf-1 and then blocked the phosphorylation of Rb, could have inhibited the HC regeneration from supporting cell proliferation. Our results indicate that Rb-Raf-1 interaction plays an important role in spontaneous HC regeneration in zebrafish.
authors: Department of Otolaryngology, Eye and ENT Hospital, Fudan University, Shanghai, PR China.
authors: Qin
authors: Q
authors: Lin
authors: Wenyan
authors: W
authors: Li
authors: Yan
authors: Y
authors: Chen
authors: Shan
authors: S
authors: Sun
authors: Huawei
authors: H
authors: Li
mesh_terms: D000818:Animals; D006198:Hair Cells, Auditory / Q000378:metabolism; D007150:Immunohistochemistry; D053403:Lateral Line System / Q000378:metabolism*; D008465:Mechanoreceptors / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology*; D019908:Proto-Oncogene Proteins c-raf / Q000378:metabolism*; D016160:Retinoblastoma Protein / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D016160:Retinoblastoma Protein; D019908:Proto-Oncogene Proteins c-raf