title: Tissue-specific upregulation of MDS/EVI gene transcripts in the intestine by thyroid hormone during Xenopus metamorphosis.
abstract: BACKGROUND
  Intestinal remodeling during amphibian metamorphosis resembles the maturation of the adult intestine during mammalian postembryonic development when the adult epithelial self-renewing system is established under the influence of high concentrations of plasma thyroid hormone (T3). This process involves de novo formation and subsequent proliferation and differentiation of the adult stem cells.
  
  
  RESULTS
  The T3-dependence of the formation of adult intestinal stem cell during Xenopus laevis metamorphosis offers a unique opportunity to identify genes likely important for adult organ-specific stem cell development. We have cloned and characterized the ectopic viral integration site 1 (EVI) and its variant myelodysplastic syndrome 1 (MDS)/EVI generated via transcription from the upstream MDS promoter and alternative splicing. EVI and MDS/EVI have been implicated in a number of cancers including breast, leukemia, ovarian, and intestinal cancers. We show that EVI and MDS/EVI transcripts are upregulated by T3 in the epithelium but not the rest of the intestine in Xenopus laevis when adult stem cells are forming in the epithelium.
  
  
  CONCLUSIONS
  Our results suggest that EVI and MDS/EVI are likely involved in the development and/or proliferation of newly forming adult intestinal epithelial cells.
authors: Section on Molecular Morphogenesis, Program in Cellular Regulation and Metabolism (PCRM), Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, Maryland, United States of America.
authors: Thomas C
authors: TC
authors: Miller
authors: Guihong
authors: G
authors: Sun
authors: Takashi
authors: T
authors: Hasebe
authors: Liezhen
authors: L
authors: Fu
authors: Rachel A
authors: RA
authors: Heimeier
authors: Biswajit
authors: B
authors: Das
authors: Atsuko
authors: A
authors: Ishizuya-Oka
authors: Yun-Bo
authors: YB
authors: Shi
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D023061:Gene Order; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects*; D008675:Metamorphosis, Biological / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D013963:Thyroid Hormones / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014284:Triiodothyronine / Q000494:pharmacology; D014982:Xenopus laevis / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D013963:Thyroid Hormones; D014157:Transcription Factors; D014284:Triiodothyronine
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