title: [Expression of TNF-alpha in lung tissue of rats with lung injury induced by brain ischemia].
abstract: OBJECTIVE
  To investigate the expression of tumor necrosis factor-alpha (TNF-alpha) in the lung tissue of rats with lung injury induced by brain ischemia.
  
  
  METHODS
  The rat model of lung injury induced by brain ischemia was established. At 24 h, 48 h, 72 h after brain ischemia, lung tissues were harvested from each rats, the expressions of TNF-alpha mRNA and protein and its distributions in the lung tissue were measured by the methods of RT-PCR (n=8), Western blot (n=8), and immunohistochemistry (n=5), respectively.
  
  
  RESULTS
  The increase of TNF-alpha mRNA and protein levels were found in the lung tissues after brain ischemia, when compared with sham group (P<0.05). The morphological study with immunohistochemical staining observed TNF-alpha positive reaction in epithelial cells and some macrophages in the lung tissues after brain ischemia.
  
  
  CONCLUSIONS
  The expression of TNF-alpha in the lung tissue could be upregulated in the rats with brain ischemia.
authors: Department of Respiration, Affiliated Kunhua Hospital of Kunming Science and Technology University, Kunming 650032, China.
authors: Feng
authors: F
authors: Liao
authors: Qi-Qin
authors: QQ
authors: Dan
authors: Run-Fen
authors: RF
authors: Du
authors: Jin-Tao
authors: JT
authors: Li
authors: Yun-Hui
authors: YH
authors: Zhang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000378:metabolism*; D055370:Lung Injury / Q000209:etiology / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha