title: Thermosensitive hydrogel made of ferulic acid-gelatin and chitosan glycerophosphate.
abstract: Reactive oxygen species-induced oxidative stress is involved in apoptosis of nucleus pulposus (NP) cells that can alter cellular phenotype and accelerate disc degeneration. Ferulic acid (FA) possesses an excellent antioxidant and anti-inflammatory properties. In the study, we developed the thermosensitive FA-gelatin/chitosan/glycerol phosphate (FA-G/C/GP) hydrogel which was applied as a sustained release system of FA to treat NP cells from the damage caused by oxidative stress. The gelation temperature of the FA-G/C/GP hydrogel was 32.17 °C. NP cells submitted to oxidative stress promoted by H(2)O(2), and post-treated with FA-G/C/GP exhibited down-regulation of MMP-3 and up-regulation aggrecan and type II collagen in mRNA level. The sulfated-glycosaminoglycan production was increased and the apoptosis was inhibited in the post-treatment group. The results suggest that the thermosensitive FA-G/C/GP hydrogel can treat NP cells from the damage caused by oxidative stress and may apply in minimally invasive surgery for NP regeneration.
authors: Institute of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, No. 1, Jen Ai Road, Section 1, Taipei, 10051, Taiwan. d97548005@ntu.edu.tw
authors: Yung-Hsin
authors: YH
authors: Cheng
authors: Shu-Hua
authors: SH
authors: Yang
authors: Chia-Ching
authors: CC
authors: Liu
authors: Amit
authors: A
authors: Gefen
authors: Feng-Huei
authors: FH
authors: Lin
mesh_terms: D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D003373:Coumaric Acids / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D005780:Gelatin / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D005994:Glycerophosphates / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000096:biosynthesis; D020100:Hydrogels / Q000737:chemistry* / Q000633:toxicity; D007403:Intervertebral Disc / Q000166:cytology; D012212:Rheology; D013696:Temperature; D014302:Trinitrobenzenesulfonic Acid / Q000737:chemistry
chemical_list: D003373:Coumaric Acids; D004337:Drug Carriers; D005994:Glycerophosphates; D006025:Glycosaminoglycans; D020100:Hydrogels; C013786:A73025; D014302:Trinitrobenzenesulfonic Acid; D005780:Gelatin; D048271:Chitosan; C004999:ferulic acid; D053148:Caspase 3