title: The role of autophagy in genome stability through suppression of abnormal mitosis under starvation.
abstract: The coordination of subcellular processes during adaptation to environmental change is a key feature of biological systems. Starvation of essential nutrients slows cell cycling and ultimately causes G1 arrest, and nitrogen starvation delays G2/M progression. Here, we show that budding yeast cells can be efficiently returned to the G1 phase under starvation conditions in an autophagy-dependent manner. Starvation attenuates TORC1 activity, causing a G2/M delay in a Swe1-dependent checkpoint mechanism, and starvation-induced autophagy assists in the recovery from a G2/M delay by supplying amino acids required for cell growth. Persistent delay of the cell cycle by a deficiency in autophagy causes aberrant nuclear division without sufficient cell growth, leading to an increased frequency in aneuploidy after refeeding the nitrogen source. Our data establish the role of autophagy in genome stability through modulation of cell division under conditions that repress cell growth.
authors: Department of Nanobiology, Graduate School of Advanced Integration Science, Chiba University, Inage-ku, Chiba, Japan.
authors: Aiko
authors: A
authors: Matsui
authors: Yoshiaki
authors: Y
authors: Kamada
authors: Akira
authors: A
authors: Matsuura
mesh_terms: D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D000782:Aneuploidy; D001343:Autophagy / Q000235:genetics*; D049109:Cell Proliferation; D016193:G1 Phase / Q000235:genetics; D016195:G2 Phase / Q000235:genetics; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics*; D042822:Genomic Instability; D008938:Mitosis; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D029701:Saccharomyces cerevisiae Proteins; C561842:TORC1 protein complex, S cerevisiae; D014157:Transcription Factors; D009584:Nitrogen
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