title: Safety evaluation of daidzein in laying hens: part I. Effects on laying performance, clinical blood parameters, and organs development.
abstract: Daidzein, an estrogen-like product, becomes increasingly popular as a dietary supplement, particularly for postpeak-estrus animals seeking a safe natural alternative to play a role of estrogen. However, there is little available safety data of it for raisers and consumers. A subchronic laying hen safety study was conducted to examine if the high-dose daidzein could affect the safety of hens selves, including laying performance, clinical blood parameters and organs development. Seven hundred and sixty-eight 56-week-old Hyline Brown were randomly assigned to 4 groups with 8 replicates of 24 birds each and 3weeks later fed diets supplemented with 0, 10, 50 and 100mg of daidzein/kg for 12weeks. The mortality was significantly decreased (P<0.05). No treatment related adverse clinical signs were observed. Mean egg production, egg mass and feed conversion of whole experiment period was significantly influenced by dietary daidzein supplement (P<0.05), showing significant quadratic response to increasing dietary daidzein supplement (P=0.029, P=0.003 and P=0.019, respectively). There was no statistically significant changes in haematology (P>0.05). In clinical chemistry parameters, total protein, total cholesterol, calcium and phosphorus were significantly affected by dietary daidzein supplement (P<0.05). The no observed adverse effect level (NOAEL) is considered to be 50mg/kg.
authors: Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China.
authors: S R
authors: SR
authors: Shi
authors: H
authors: H
authors: Gu
authors: L L
authors: LL
authors: Chang
authors: Z Y
authors: ZY
authors: Wang
authors: H B
authors: HB
authors: Tong
authors: J M
authors: JM
authors: Zou
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D004531:Eggs; D007529:Isoflavones / Q000097:blood / Q000494:pharmacology*; D019055:No-Observed-Adverse-Effect Level; D015203:Reproducibility of Results
chemical_list: D007529:Isoflavones; C004742:daidzein; D002118:Calcium