title: Oligodeoxyribonucleotides derived from salmon sperm DNA: an alternative to defibrotide.
abstract: Defibrotide is a single-stranded nucleic acid polymer originally derived from porcine mucosa. Cheap salmon sperm DNA is commercially available and widely used in drug production. In this study, oligodeoxyribonucleotides were successfully obtained from the controlled depolymerization of salmon sperm DNA. The obtained product shared similar chemical and biological properties with defibrotide produced by Gentium SpA, Italy. It was also found that oligodeoxyribonucleotides derived from non-mammalian origins could also directly stimulate tissue plasminogen activator (t-PA) release from cultured human endothelial cells, and enhance fibrinolytic activity in the rabbit.
authors: Shenzhen Prevention and Treatment Center for Occupational Disease, Shenzhen, Guangdong 518001, China. hcy_sypu@hotmail.com
authors: Chang-Ye
authors: CY
authors: Hui
authors: Yan
authors: Y
authors: Guo
authors: Xi
authors: X
authors: Zhang
authors: Jian-Hua
authors: JH
authors: Shao
authors: Xue-Qin
authors: XQ
authors: Yang
authors: Wen
authors: W
authors: Zhang
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011089:Polydeoxyribonucleotides / Q000494:pharmacology; D011817:Rabbits; D012474:Salmon / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D010959:Tissue Plasminogen Activator / Q000378:metabolism
chemical_list: D005343:Fibrinolytic Agents; D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides; C036901:defibrotide; D004247:DNA; D010959:Tissue Plasminogen Activator