title: Synaptic ribbons influence the size and frequency of miniature-like evoked postsynaptic currents.
abstract: Nonspiking cells of several sensory systems respond to stimuli with graded changes in neurotransmitter release and possess specialized synaptic ribbons. Here, we show that manipulations to synaptic ribbons caused dramatic effects on mEPSC-like (mlEPSC) amplitude and frequency. Damage to rod-bipolar cell ribbons using fluorophore-assisted light inactivation resulted in the immediate reduction of mlEPSC amplitude and frequency, whereas the first evoked response after damage remained largely intact. The reduction in amplitude could not be recovered by increasing release frequency after ribbon damage. In parallel experiments, we looked at mlEPSCs from cones of hibernating ground squirrels, which exhibit dramatically smaller ribbons than awake animals. Fewer and smaller mlEPSCs were observed postsynaptic to cones from hibernating animals, although depolarized cones were able to generate larger mlEPSCs. Our results indicate that ribbon size may influence mlEPSC frequency and support a role for ribbons in coordinating multivesicular release.
authors: Department of Cellular and Molecular Physiology, Yale University School of Medicine, Sterling Hall of Medicine, Room B147, New Haven, CT 06520, USA.
authors: Bhupesh
authors: B
authors: Mehta
authors: Josefin
authors: J
authors: Snellman
authors: Shan
authors: S
authors: Chen
authors: Wei
authors: W
authors: Li
authors: David
authors: D
authors: Zenisek
mesh_terms: D000429:Alcohol Oxidoreductases; D000613:Aminobutyrates / Q000494:pharmacology; D000818:Animals; D001703:Biophysics; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology*; D018756:GABA Antagonists / Q000494:pharmacology; D018684:Glycine Agents / Q000494:pharmacology; D006605:Hibernation / Q000502:physiology; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D018408:Patch-Clamp Techniques; D010721:Phosphinic Acids / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism; D010852:Picrotoxin / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D018091:Receptors, AMPA / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D012589:Sciuridae; D013331:Strychnine / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D014795:Visual Pathways / Q000502:physiology; D014851:Wakefulness / Q000502:physiology
chemical_list: D000429:Alcohol Oxidoreductases; D000613:Aminobutyrates; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D018690:Excitatory Amino Acid Agonists; D018756:GABA Antagonists; D018684:Glycine Agents; D010721:Phosphinic Acids; D010750:Phosphoproteins; D010852:Picrotoxin; D011725:Pyridines; D018091:Receptors, AMPA; D013331:Strychnine; C101879:(1,2,5,6-tetrahydropyridin-4-yl)methylphosphinic acid; C104724:glutamate receptor ionotropic, AMPA 4; C111341:Ctbp2 protein, mouse; C012729:2-amino-4-phosphonobutyric acid
references: Neuron. 2003 Mar 6;37(5):775-86
references: 12628168
references: J Neurosci. 2003 Nov 26;23(34):10923-33
references: 14645488
references: Nat Neurosci. 2004 Aug;7(8):826-33
references: 15235608
references: J Neurosci. 2011 Jul 27;31(30):11003-15
references: 21795549
references: Nature. 2005 Apr 14;434(7035):889-94
references: 15829963
references: J Physiol. 2009 Jun 1;587(Pt 11):2443-55
references: 19332496
references: J Physiol. 1967 Nov;193(2):419-32
references: 6065887
references: Nat Rev Neurosci. 2010 Dec;11(12):812-22
references: 21045860
references: J Physiol. 2002 Jul 1;542(Pt 1):147-65
references: 12096058
references: J Neurosci. 2006 Jan 4;26(1):210-6
references: 16399689
references: Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7520-5
references: 10377447
references: Science. 1981 Jan 9;211(4478):182-5
references: 6255566
references: J Neurosci. 2010 Mar 24;30(12):4210-20
references: 20335456
references: Science. 1974 Oct 4;186(4158):47-9
references: 4417736
references: J Neurosci. 1998 Jun 15;18(12):4532-47
references: 9614230
references: Nat Neurosci. 2002 Feb;5(2):147-54
references: 11802170
references: Neuron. 2011 Aug 11;71(3):474-87
references: 21835344
references: J Physiol. 1952 May;117(1):109-28
references: 14946732
references: Neuron. 1994 Jan;12(1):51-9
references: 7507341
references: J Neurosci. 2001 Apr 1;21(7):2330-42
references: 11264308
references: J Physiol. 2003 Jun 15;549(Pt 3):759-74
references: 12702738
references: J Neurosci. 2011 Apr 6;31(14):5378-82
references: 21471372
references: Physiology (Bethesda). 2011 Feb;26(1):45-53
references: 21357902
references: Neuron. 2001 Oct 25;32(2):301-13
references: 11683999
references: J Neurosci. 2008 May 21;28(21):5403-11
references: 18495874
references: Nat Neurosci. 2009 Mar;12(3):303-10
references: 19219039
references: J Neurosci. 2004 Nov 3;24(44):9752-9
references: 15525760
references: J Neurosci. 2010 Sep 8;30(36):11885-95
references: 20826653
references: J Neurosci. 1988 Feb;8(2):623-42
references: 2828567
references: J Neurosci. 2001 Nov 1;21(21):8616-23
references: 11606649
references: Nat Neurosci. 2011 Oct 23;14(12):1555-61
references: 22019730
references: Neuron. 2010 Nov 18;68(4):724-38
references: 21092861
references: J Neurosci. 2010 Jun 2;30(22):7587-97
references: 20519533
references: J Neurophysiol. 2006 May;95(5):3191-8
references: 16452253
references: Methods Cell Biol. 2007;82:335-54
references: 17586263
references: J Neurosci. 2006 Nov 29;26(48):12487-96
references: 17135411
references: Neuroscientist. 2009 Dec;15(6):611-24
references: 19700740
references: J Neurosci. 2000 Jan 15;20(2):568-78
references: 10632586
references: J Neurophysiol. 2006 Oct;96(4):2025-33
references: 16738212
references: Invest Ophthalmol Vis Sci. 1977 Sep;16(9):815-40
references: 893032
references: Curr Opin Neurobiol. 2011 Apr;21(2):275-82
references: 21334193
references: Neuron. 2012 Jan 12;73(1):121-34
references: 22243751
references: Neuron. 2000 Dec;28(3):857-72
references: 11163272
references: Nat Neurosci. 2011 Jul 24;14(9):1135-41
references: 21785435
references: J Neurosci. 2009 Jun 10;29(23):7558-68
references: 19515924
references: J Neurosci. 2011 Nov 16;31(46):16637-50
references: 22090491
references: Nat Neurosci. 2006 May;9(5):669-75
references: 16617341