title: Effect of carboxymethylated chitosan on the biosynthesis of NGF and activation of the Wnt/β-catenin signaling pathway in the proliferation of Schwann cells.
abstract: The proliferation of Schwann cells around injured peripheral nerves supports the process of Wallerian degeneration and is critical for axonal regeneration. In this publication, carboxymethylated chitosan (CMCS) was studied to determine its capacity (i) to induce proliferation and secretion of nerve growth factor (NGF) and (ii) to activate Wingless-type(Wnt) protein/β-catenin signaling pathways in rat Schwann cells. CMCS was found to induce Schwann cell proliferation and NGF synthesis in Schwann cell in a dose and time dependent manner. CMCS was shown to activate factors in the Wnt/β-catenin signaling pathway, including Dvl-1, β-catenin, Tcf4, Lef1, C-myc, and Cyclin D1 which are active in the proliferation of Schwann cells and biosynthesis of NGF of Schwann cell. Overall, this study suggests that CMCS can promote the proliferation of cultured Schwann cells and synthesis of NGF by activating the Wnt/β-catenin signaling pathway.
authors: Department of Orthopedics, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang District, Wuhan 430060, Hubei Province, People's Republic of China.
authors: Hai-Ying
authors: HY
authors: Tao
authors: Bin
authors: B
authors: He
authors: Shi-Qing
authors: SQ
authors: Liu
authors: Ai-Lin
authors: AL
authors: Wei
authors: Feng-Hua
authors: FH
authors: Tao
authors: Hai-Li
authors: HL
authors: Tao
authors: Wan-Xia
authors: WX
authors: Deng
authors: Hao-Huan
authors: HH
authors: Li
authors: Qing
authors: Q
authors: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D000072261:Dishevelled Proteins; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism; D020932:Nerve Growth Factor / Q000096:biosynthesis*; D010750:Phosphoproteins / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D000072261:Dishevelled Proteins; C000606580:Dvl1 protein, rat; C497575:Lef1 protein, rat; D051821:Lymphoid Enhancer-Binding Factor 1; D010750:Phosphoproteins; C491192:Tcf4 protein, rat; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051176:beta Catenin; D048271:Chitosan; D020932:Nerve Growth Factor