title: Splenic priming of virus-specific CD8 T cells following influenza virus infection.
abstract: In healthy individuals, influenza virus (IAV) infection generally remains localized to the epithelial cells of the respiratory tract. Previously, IAV-specific effector CD8 T cells found systemically during the course of IAV infection were thought to have been primed in lung-draining lymph nodes with subsequent migration to other tissues. However, little is known about whether other lymphoid sites participate in the generation of virus-specific CD8 T cells during localized IAV infection. Here, we present evidence of early CD8 T cell priming in the spleen following respiratory IAV infection independent of lung-draining lymph node priming of T cells. Although we found early indications of CD8 T cell activation in the lymph nodes draining the respiratory tract, we also saw evidence of virus-specific CD8 T cell activation in the spleen. Furthermore, CD8 T cells primed in the spleen differentiated into memory cells of equivalent longevity and with similar recall capacity as CD8 T cells primed in the draining lymph nodes. These data showed that the spleen contributes to the virus-specific effector and memory CD8 T cell populations that are generated in response to respiratory infection.
authors: Department of Immunology, Center for Integrative Immunology and Vaccine Research, University of Connecticut Health Center, Farmington, Connecticut, USA.
authors: Damian L
authors: DL
authors: Turner
authors: Kara L
authors: KL
authors: Bickham
authors: Donna L
authors: DL
authors: Farber
authors: Leo
authors: L
authors: Lefrançois
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000821:virology; D012141:Respiratory Tract Infections / Q000276:immunology* / Q000821:virology; D013154:Spleen / Q000276:immunology* / Q000821:virology*
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