title: Single nucleotide polymorphisms in CXCR1 gene and its association with hepatitis B infected patients in Saudi Arabia.
abstract: OBJECTIVE
  This study aims to investigate whether the SNPs of CXCR1 gene, could predict the likelihood of viral persistence and/or disease progression.
  
  
  METHODS
  We investigated the association of two different SNPs (rs2234671, and rs142978743) in 598 normal healthy controls and 662 HBV patients from a Saudi ethnic population. The HBV patients were categorized into inactive carriers (n = 428), active carriers (n = 162), cirrhosis (n = 54) and Cirrhosis-HCC (n = 18) sub-groups. Genetic variants in CXCR1 were determined by polymerase chain reaction (PCR)-based DNA direct sequencing.
  
  
  RESULTS
  The frequency of the risk allele 'C' for the SNP, rs2234671 was found to be insignificant when the patient group was compared to the uninfected control group, however, a significant distribution of the allele 'C' of rs2234671 was observed among active HBV carriers + cirrhosis + cirrhosis - HCC vs. inactive HBV carriers with an OR = 1.631 (95% C.I. 1.016-2.616) and p = 0.032. However, no significant association was observed for rs142978743 when the distribution of risk allele was analyzed among the different patient groups (i.e. inactive carriers, active carriers, cirrhosis and HCC). Furthermore, the most common haplotype, Haplo-1 (AG), was found to have an insignificant frequency distribution between HBV cases and controls, while the same haplotype was found to be significantly distributed when active carriers + cirrhosis + cirrhosis - HCC patients were compared to inactive HBV carriers with a frequency of 0.938 and p = 0.0315. Haplo-2 (AC) was also found to be significantly associated with a frequency of 0.058 and p = 0.0163.
  
  
  CONCLUSIONS
  The CXCR1 polymorphism, rs2234671 was found to be associated with chronic HBV infection and may play a role in disease activity.
authors: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors: Fahad N
authors: FN
authors: Almajhdi
authors: Mohammed
authors: M
authors: Al-Ahdal
authors: Ayman A
authors: AA
authors: Abdo
authors: Faisal M
authors: FM
authors: Sanai
authors: Mashael
authors: M
authors: Al-Anazi
authors: Nisreen
authors: N
authors: Khalaf
authors: Nisha A
authors: NA
authors: Viswan
authors: Hamad
authors: H
authors: Al-Ashgar
authors: Khalid
authors: K
authors: Al-Kahtani
authors: Hind
authors: H
authors: Al-Humaidan
authors: Riham
authors: R
authors: Al-Swayeh
authors: Zahid
authors: Z
authors: Hussain
authors: Saud
authors: S
authors: Alarifi
authors: Majid
authors: M
authors: Al-Okail
authors: Ahmed
authors: A
authors: Al-Qahtani
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000235:genetics / Q000276:immunology; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D018450:Disease Progression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006509:Hepatitis B / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008103:Liver Cirrhosis / Q000453:epidemiology / Q000235:genetics / Q000276:immunology; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000276:immunology; D016017:Odds Ratio; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D023062:Receptors, Interleukin-8A / Q000235:genetics*; D012307:Risk Factors; D012529:Saudi Arabia / Q000453:epidemiology; D017422:Sequence Analysis, DNA
chemical_list: D023062:Receptors, Interleukin-8A