title: The use of transient expression systems for the rapid production of virus-like particles in plants.
abstract: Advances in transient expression technologies have allowed the production of milligram quantities of proteins within a matter of days using only small amounts (tens of grams) of plant tissue. Among the proteins that have been produced using this approach are the structural proteins of viruses which are capable of forming virus-like particles (VLPs). As such particulate structures are potent stimulators of the immune system, they are excellent vaccine candidates both in their own right and as carriers of additional immunogenic sequences. VLPs of varying complexity derived from a variety of animal viruses have been successfully transiently expressed in plants and their immunological properties assessed. Generally, the plant-produced VLPs were found to have the expected antigenicity and immunogenicity. In several cases, including an M2e-based influenza vaccine candidate, the plant-expressed VLPs have been shown to be capable of stimulating protective immunity. These findings raise the prospect that low-cost plant-produced vaccines could be developed for both veterinary and human use.
authors: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. Eva.Thuenemann@ifr.ac.uk
authors: Eva C
authors: EC
authors: Thuenemann
authors: Paolo
authors: P
authors: Lenzi
authors: Andrew J
authors: AJ
authors: Love
authors: Michael
authors: M
authors: Taliansky
authors: Martina
authors: M
authors: Bécares
authors: Sonia
authors: S
authors: Zuñiga
authors: Luis
authors: L
authors: Enjuanes
authors: Gergana G
authors: GG
authors: Zahmanova
authors: Ivan N
authors: IN
authors: Minkov
authors: Slavica
authors: S
authors: Matić
authors: Emanuela
authors: E
authors: Noris
authors: Ann
authors: A
authors: Meyers
authors: Alta
authors: A
authors: Hattingh
authors: Edward P
authors: EP
authors: Rybicki
authors: Oleg I
authors: OI
authors: Kiselev
authors: Nikolai V
authors: NV
authors: Ravin
authors: Michael A
authors: MA
authors: Eldarov
authors: Konstantin G
authors: KG
authors: Skryabin
authors: George P
authors: GP
authors: Lomonossoff
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D019149:Bioreactors; D006801:Humans; D010940:Plant Proteins / Q000378:metabolism*; D013997:Time Factors; D058425:Vaccines, Virus-Like Particle / Q000096:biosynthesis* / Q000191:economics / Q000276:immunology; D014764:Viral Proteins / Q000378:metabolism*; D014765:Viral Vaccines / Q000191:economics / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D010940:Plant Proteins; D058425:Vaccines, Virus-Like Particle; D014764:Viral Proteins; D014765:Viral Vaccines