title: Pressurized whey protein can limit bacterial burden and protein oxidation in Pseudomonas aeruginosa lung infection.
abstract: BACKGROUND
  Lung infection caused by Pseudomonas aeruginosa is associated with an exuberant inflammatory response, oxidative stress, and lung damage. Whey protein is a rich source of cysteine, and anti-inflammatory and immune-enhancing peptides. Anti-inflammatory and antioxidant properties of whey are augmented by hyperbaric pressure treatment. In this study, we tested whether dietary supplementation with pressurized whey protein enhances the host ability to clear P. aeruginosa infection compared with native (i.e., unpressurized) whey.
  
  
  METHODS
  Using a minimally invasive, non-lethal model of murine (female C57Bl/6) model of P. aeruginosa infection (mucoid strain embedded in agar beads), we studied kinetics of infection, inflammation, and oxidative stress at d 1, 3, and 7 postinfection. A parallel set of mice were fed for 4 wk a semipurified diet containing either native or pressurized whey and subsequently infected with P. aeruginosa. In these mice, the parameters mentioned previously were studied at d 1 and 3 postinfection.
  
  
  RESULTS
  Infection with P. aeruginosa resulted in inflammation and protein oxidation sustained beyond bacterial clearance. Animals that were fed pressurized whey had fewer bacteria at day 3 than mice on native whey. Weight loss or broncho-alveolar lavage cell content were comparable. Airway protein oxidation was attenuated, whereas airway leukocyte bacterial killing ability and oxidative burst in response to opsonized bacteria were increased in the pressurized whey-fed animals.
  
  
  CONCLUSIONS
  Use of nutritionally derived substances with anti-inflammatory and antioxidant properties, such as pressurized whey, aids in limiting airway bacterial infection, particularly, under conditions of ongoing oxidative stress.
authors: Division of Pediatric Respiratory Medicine, Montreal Children's Hospital-McGill University Health Centre, Montreal, QC, Canada.
authors: Osama A
authors: OA
authors: Kishta
authors: Michele
authors: M
authors: Iskandar
authors: Nurlan
authors: N
authors: Dauletbaev
authors: Stan
authors: S
authors: Kubow
authors: Larry C
authors: LC
authors: Lands
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D018893:Bronchoalveolar Lavage; D003545:Cysteine / Q000494:pharmacology; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008894:Milk Proteins / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa; D015431:Weight Loss; D000067816:Whey Proteins
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D008894:Milk Proteins; D000067816:Whey Proteins; D005978:Glutathione; D003545:Cysteine