title: Chronic ethanol exposure increases cytochrome P-450 and decreases activated in blocked unfolded protein response gene family transcripts in caenorhabditis elegans.
abstract: Ethanol is a widely consumed and rapidly absorbed toxin. While the physiological effects of ethanol consumption are well known, the underlying biochemical and molecular changes at the gene expression level in whole animals remain obscure. We exposed the model organism Caenorhabditis elegans to 0.2 M ethanol from the embryo to L4 larva stage and assayed gene expression changes in whole animals using RNA-Seq and quantitative real-time PCR. We observed gene expression changes in 1122 genes (411 up, 711 down). Cytochrome P-450 (CYP) gene family members (12 of 78) were upregulated, whereas activated in blocked unfolded protein response (ABU) (7 of 15) were downregulated. Other detoxification gene family members were also regulated including four glutathione-S-transferases and three flavin monooxygenases. The results presented show specific gene expression changes following chronic ethanol exposure in C. elegans that indicate both persistent upregulation of detoxification response genes and downregulation of endoplasmic reticulum stress pathway genes.
authors: A. I. Virtanen Institute for Molecular Sciences, Department of Neurobiology, University of Eastern Finland, Yliopistonranta 1E, 70210, Kuopio, Finland.
authors: Juhani
authors: J
authors: Peltonen
authors: Vuokko
authors: V
authors: Aarnio
authors: Liisa
authors: L
authors: Heikkinen
authors: Merja
authors: M
authors: Lakso
authors: Garry
authors: G
authors: Wong
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D056811:Unfolded Protein Response / Q000187:drug effects
chemical_list: D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System