title: Suppression of CLIC4/mtCLIC enhances hydrogen peroxide-induced apoptosis             in C6 glioma cells.
abstract: CLIC4/mtCLIC (referred to here as CLIC4) is one of the seven-member family             of chloride intracellular channels (CLIC). CLIC4 localizes to the mitochondria,             nucleus, cytoplasm and other organellular compartments and participates in the             apoptotic response to stress. However, the role of CLIC4 in oxidative stress and             apoptosis is not well understood. In this study, we showed the important role             of CLIC4 in apoptosis of C6 glioma cells induced by hydrogen peroxide (H2O2).             Our results showed that CLIC4 protein expression was upregulated following H2O2-induced             C6 cell apoptosis. The upregulation of CLIC4 protein expression was paralleled             with an increased Bax/Bcl-2 ratio, cytochrome c and cleaved caspase-3 protein             expression upon H2O2-induced C6 cell apoptosis. Suppression of CLIC4 expression             by RNA interference enhanced cell apoptosis, but the ratio of Bax/Bcl-2 was not             involved in this process. Dissipation of mitochondrial membrane potential and             nuclear translocation of CLIC4 were involved in the activation of apoptosis induced             by H2O2. Our data indicate that CLIC4 protein may be a key element in the apoptotic             response to oxidative stress.
authors: Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, PR China.
authors: Ye
authors: Y
authors: Xu
authors: Jinsong
authors: J
authors: Kang
authors: Zhaoxin
authors: Z
authors: Yuan
authors: Hongyan
authors: H
authors: Li
authors: Jing
authors: J
authors: Su
authors: Yang
authors: Y
authors: Li
authors: Xiaoxia
authors: X
authors: Kong
authors: Hongyu
authors: H
authors: Zhang
authors: Weiwei
authors: W
authors: Wang
authors: Liankun
authors: L
authors: Sun
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D018118:Chloride Channels / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress; D021381:Protein Transport / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C464459:CLIC4 protein, human; D018118:Chloride Channels; D006861:Hydrogen Peroxide