title: Comparison of betanodavirus replication efficiency in ten Indian fish cell lines.
abstract: Ten cell lines established from Indian marine, brackishwater and freshwater fish were tested for their susceptibility to fish nodavirus. In addition, the efficiency of betanodavirus replication was tested in these cell lines. Multiple vacuolation, a typical cytopathic effect for virus infection, was observed in infected SISK, SISS, SIGE and ICF cells. Infection of the different fish cell lines was confirmed by RT-PCR, immunodot blot assay and indirect ELISA. The virus concentration in culture supernatant collected from infected sea bass and grouper cell lines increased progressively from 10(3) at day 1 postinfection to 10(8) TCID50 ml(-1) at day 9. The amount of virus in different cell lines was also quantified by real-time PCR. These results indicate the suitability of the SISK, SISS, and SIGE cell lines for fish nodavirus propagation for developing viral diagnostics and vaccines.
authors: OIE Reference Laboratory for WTD, PG and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509 Vellore District, Tamilnadu, India.
authors: V
authors: V
authors: Sarath Babu
authors: S
authors: S
authors: Abdul Majeed
authors: K S N
authors: KS
authors: Nambi
authors: G
authors: G
authors: Taju
authors: N
authors: N
authors: Madan
authors: N
authors: N
authors: Sundar Raj
authors: A S
authors: AS
authors: Sahul Hameed
mesh_terms: D000818:Animals; D001492:Bass / Q000821:virology; D002460:Cell Line; D005393:Fish Diseases / Q000821:virology*; D005399:Fishes / Q000821:virology; D015151:Immunoblotting / Q000662:veterinary; D007194:India; D030381:Nodaviridae / Q000502:physiology*; D012327:RNA Virus Infections / Q000662:veterinary* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D014779:Virus Replication / Q000502:physiology*