title: [Effect of tetramethylpyrazine on Nogo gene regulation of the proliferation in cardiac fibroblast].
abstract: OBJECTIVE
  To investigate the effects of different doses of Tetramethylpyrazine (TMP) on collagen synthesis and Nogo expression in cardiac fibroblasts and the potential role of Nogo in myocardial fibrosis.
  
  
  METHODS
  The cultured neonatal rat cardiac fibroblasts were induced by angiotensin II and TMP at the same time. The mRNA expression of Nogo-A, Nogo-B and collagen I were detected with real-time fluorescence quantitative PCR. And Nogo protein was semi-quantitatively detected by using Western blot method.
  
  
  RESULTS
  Under the intervention of TMP, the increased mRNA expression of Nogo-A and collagen I induced by angiotensin II were decreased, while the decreased mRNA expression of Nogo-B was increased. Compared with the control group, the expression of Nogo-A protein and Nogo-B protein showed similar changes as evaluated with Western blot.
  
  
  CONCLUSIONS
  TMP could inhibit collagen synthesis in cardiac fibroblast induced by angiotensin II. The accompanying differential expression of Nogo-A and Nogo-B indicated that the anti-fibrosis effect of TMP might be related to the regulation effects of Nogo gene.
authors: Department of Emergency, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Hue
authors: H
authors: Nie
authors: Xun
authors: X
authors: Ran
authors: Zhi
authors: Z
authors: Zeng
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005786:Gene Expression Regulation; D008297:Male; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects*; D000070798:Nogo Proteins; D011719:Pyrazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024042:Collagen Type I; D009185:Myelin Proteins; D000070798:Nogo Proteins; D011719:Pyrazines; D012333:RNA, Messenger; C000603197:Rtn4 protein, rat; D000804:Angiotensin II; C017953:tetramethylpyrazine