title: Down-regulation of miRNA-30a in human plasma is a novel marker for breast cancer.
abstract: The present study was to evaluate the value of miRNA-30a in plasma as potential tumor marker in detecting breast cancer (BC). Using a novel approach to extract miRNA-30a from the plasma followed by real-time quantitative polymerase chain reaction (RQ-PCR) analysis, levels of miRNA-30a were quantified in plasma specimens of 100 BCs and 64 age-matched and disease-free healthy controls (HC). And we compared the diagnostic value of plasma miRNA-30a with conventional circulating tumor markers CA153 and CEA. The median levels of miRNA-30a were significantly lower in preoperative BC than those in HC (P < 0.001). The levels of CEA and CA153 were all significantly higher in preoperative BC compared with those in HC (P = 0.008 and P = 0.001, respectively), and only the level of CA153 decreased in postoperative BC compared with preoperative BC (P = 0.015). ROC analysis showed the sensitivity and specificity of miRNA-30a for BC diagnosis at 74.0 and 65.6 %, respectively, whereas the sensitivities of CEA and CA153 were 12.0 and 14.0 %, respectively. The status of ER and triple-negative BC was significantly associated with miRNA-30a level (P = 0.007 and P = 0.005, respectively). And no other clinicopathological features were found to had significant difference. Our findings suggest that plasma miRNA-30a decreased in patients with BC and has great potential to use as novel biomarkers for BC diagnosis.
authors: Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical College, No.2, Fu Xue Road, WenZhou City 325000, Zhejiang Province, People's Republic of China.
authors: Rui-chao
authors: RC
authors: Zeng
authors: Wei
authors: W
authors: Zhang
authors: Xing-qiang
authors: XQ
authors: Yan
authors: Zhi-qiang
authors: ZQ
authors: Ye
authors: En-dong
authors: ED
authors: Chen
authors: Du-ping
authors: DP
authors: Huang
authors: Xiao-hua
authors: XH
authors: Zhang
authors: Guan-li
authors: GL
authors: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002272:Carcinoembryonic Antigen / Q000097:blood; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D008969:Molecular Sequence Data; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; C546086:MIRN30b microRNA, human; D035683:MicroRNAs
references: Ann Intern Med. 2009 Nov 17;151(10):716-26, W-236
references: 19920272
references: Br J Cancer. 1997;75(9):1324-9
references: 9155053
references: Clin Cancer Res. 2007 Aug 1;13(15 Pt 1):4429-34
references: 17671126
references: Br J Haematol. 2008 May;141(5):672-5
references: 18318758
references: Br J Cancer. 1995 Jun;71(6):1288-91
references: 7779725
references: Clin Cancer Res. 1996 Sep;2(9):1601-6
references: 9816339
references: Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15524-9
references: 12434020
references: World J Urol. 2011 Jun;29(3):367-73
references: 21229250
references: Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10513-8
references: 18663219
references: J Natl Cancer Inst. 2008 Jun 18;100(12):876-87
references: 18544742
references: Asian Pac J Cancer Prev. 2010;11(1):157-9
references: 20593949
references: Cancer Res. 2005 Aug 15;65(16):7065-70
references: 16103053
references: Int J Mol Med. 2012 Sep;30(3):680-6
references: 22692639
references: Neurobiol Dis. 2008 Mar;29(3):438-45
references: 18082412
references: Int J Cancer. 2010 Jul 1;127(1):118-26
references: 19876917
references: Gynecol Oncol. 2009 Jan;112(1):55-9
references: 18954897
references: Asian Pac J Cancer Prev. 2012;13(5):2399-403
references: 22901228
references: Breast Cancer Res Treat. 2012 Aug;134(3):1081-93
references: 22476851
references: Clin Cancer Res. 2011 Nov 1;17(21):6802-11
references: 21890451
references: Leukemia. 2012 Aug;26(8):1752-60
references: 22395361
references: Int J Clin Oncol. 2008 Oct;13(5):447-51
references: 18946756
references: Cancer Res. 2008 May 1;68(9):3108-14
references: 18451135
references: Ann Intern Med. 2009 Nov 17;151(10):738-47
references: 19920274
references: Cancer. 1994 Sep 1;74(5):1615-20
references: 7520352
references: Oncogene. 2008 Apr 3;27(15):2128-36
references: 17968323
references: Tumour Biol. 2011 Jun;32(3):583-8
references: 21274675
references: BMC Res Notes. 2009 May 19;2:89
references: 19454029
references: Mol Carcinog. 2011 Feb;50(2):136-42
references: 21229610
references: Eur J Gynaecol Oncol. 2008;29(5):459-61
references: 19051812
references: Breast Cancer Res Treat. 2011 May;127(1):43-51
references: 20490652
references: Clin Chem. 2010 Jul;56(7):1148-57
references: 20472825
references: Nucleic Acids Res. 2005 Sep 28;33(17):5394-403
references: 16192569