title: High frequency of vancomycin-resistant enterococcus faecalis in an Iranian referral children medical hospital.
abstract: BACKGROUND
  Enterococci have emerged in recent years as important nosocomial pathogens. Although most enterococcal human infections are caused by Enterococcus faecalis, studies on vancomycin resistance are usually limited to Enterococcus faecium isolates and a little is known about E. faecalis. Therefore we undertook this study to obtain information about the prevalence of vancomycin -resistant E. faecalis (VREF) and genes responsible for resistance.
  
  
  METHODS
  Ninety-one E. faecalis isolates of different patients admitted at Children's Medical Center from August 2009 to June 2010 were included in this cross-sectional study. Antimicrobial testing was performed by Kirby-Bauer disk diffusion method according to Clinical Laboratories Standards Institute (CLSI).
  
  
  RESULTS
  Among all isolates, 15 (16%) were identified as VR E. faecalis. PCR analysis revealed that all VREF isolates were positive for the vanA gene.
  
  
  CONCLUSIONS
  The present study reports the highest range of VREF in Iran. The increased frequency of VREF, as seen with rapid rise in the number of VanA isolates should be considered in infection control practices.
authors: Pediatrics Infectious Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Babak
authors: B
authors: Pourakbari
authors: Mojtaba Kamali
authors: MK
authors: Aghdam
authors: Shima
authors: S
authors: Mahmoudi
authors: Mohammad Taghi Haghi
authors: MT
authors: Ashtiani
authors: Farah
authors: F
authors: Sabouni
authors: Zahra
authors: Z
authors: Movahedi
authors: Amir Esmael
authors: AE
authors: Alyari
authors: Reihane Hosseinpour
authors: RH
authors: Sadeghi
authors: Setareh
authors: S
authors: Mamishi
keywords: E. faecalis; Iran; vancomycin resistant
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