title: A methodology for detection and quantification of esterase activity.
abstract: Carboxylesterases are important enzymes for xenobiotic metabolism and are receiving increasing attention in the context of cancer therapies. Quantification of individual carboxylesterase activity is important since protein levels do not always correlate to activity and significant interorgan, interindividual, and interspecies variations exist. Here we present a methodology enabling the specific quantification of carboxylesterase 2 activity in a pool of other esterases. Method applicability is illustrated for the evaluation of interspecies variation and for activity assessment of transfected cell extracts. The methodology can easily be adapted to the evaluation of other esterases upon careful selection of adequate substrates and/or specific inhibitors.
authors: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal. anas@itqb.unl.pt
authors: Ana L
authors: AL
authors: Simplício
authors: Ana S
authors: AS
authors: Coroadinha
authors: John F
authors: JF
authors: Gilmer
authors: Joana
authors: J
authors: Lamego
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods; D057075:Enzyme Assays / Q000379:methods; D004950:Esterases / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D057809:HEK293 Cells; D006801:Humans; D006868:Hydrolysis; D014468:Umbelliferones / Q000737:chemistry
chemical_list: D014468:Umbelliferones; C032078:4-methylumbelliferyl acetate; D004950:Esterases