title: Trichohyalin-like 1 protein, a member of fused S100 proteins, is expressed in normal and pathologic human skin.
abstract: Trichohyalin-like 1 (TCHHL1) protein is a novel member of the fused-type S100 protein gene family. The deduced amino acid sequence of TCHHL1 contains an EF-hand domain in the N-terminus, one trans-membrane domain and a nuclear localization signal. We generated specific antibodies against the C-terminus of the TCHHL1 protein and examined the expression of TCHHL1 proteins in normal and pathological human skin. An immunohistochemical study showed that TCHHL1 proteins were expressed in the basal layer of the normal epidermis. In addition, signals of TCHHL1 proteins were observed around the nuclei of cultured growing keratinocytes. Accordingly, TCHHL1 mRNA has been detected in normal skin and cultured growing keratinocytes. Furthermore, TCHHL1 proteins were strongly expressed in the peripheral areas of tumor nests in basal cell carcinomas and squamous cell carcinomas. A dramatic increase in the number of Ki67 positive cells was observed in TCHHL1-expressing areas. The expression of TCHHL1 proteins also increased in non-cancerous hyperproliferative epidermal tissues such as those of psoriasis vulgaris and lichen planus. These findings highlight the possibility that TCHHL1 proteins are expressed in growing keratinocytes of the epidermis and might be associated with the proliferation of keratinocytes.
authors: Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194, Japan.
authors: Takako
authors: T
authors: Yamakoshi
authors: Teruhiko
authors: T
authors: Makino
authors: Mati
authors: M
authors: Ur Rehman
authors: Yoko
authors: Y
authors: Yoshihisa
authors: Michiya
authors: M
authors: Sugimori
authors: Tadamichi
authors: T
authors: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D049109:Cell Proliferation; D002478:Cells, Cultured; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology; D008010:Lichen Planus / Q000378:metabolism*; D008969:Molecular Sequence Data; D011565:Psoriasis / Q000378:metabolism*; D009418:S100 Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000378:metabolism* / Q000473:pathology
chemical_list: D009418:S100 Proteins; C561436:TCHHL1 protein, human