title: Wickerhamomyces siamensis sp. nov., a novel yeast species isolated from the phylloplane in Thailand.
abstract: Strain DMKU-RK359(T), representing a novel yeast species, was isolated from the external surface of a sugar-cane leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 region of the large-subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK359(T) was assigned to a novel Wickerhamomyces species. The novel species was closest to Wickerhamomyces ciferrii, but differed from it by 0.7 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 6 % nucleotide substitutions in the ITS region. The name Wickerhamomyces siamensis sp. nov. is proposed (type strain DMKU-RK359(T)  = BCC 50732(T)  = NBRC 108900(T)  = CBS 12570(T)).
authors: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors: Rungluk
authors: R
authors: Kaewwichian
authors: NITE Biological Resource Center, Department of Biotechnology (NBRC), National Institute of Technology and Evaluation (NITE), Chiba, Japan.
authors: Hiroko
authors: H
authors: Kawasaki
authors: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors: Savitree
authors: S
authors: Limtong
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer