title: Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China.
abstract: Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006-2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.
authors: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.
authors: Jianfei
authors: J
authors: Chen
authors: Xiaozhen
authors: X
authors: Liu
authors: Hongwu
authors: H
authors: Lang
authors: Zhongtian
authors: Z
authors: Wang
authors: Da
authors: D
authors: Shi
authors: Hongyan
authors: H
authors: Shi
authors: Xin
authors: X
authors: Zhang
authors: Li
authors: L
authors: Feng
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D014644:Genetic Variation / Q000235:genetics; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000235:genetics*; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000235:genetics*; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
chemical_list: D019590:Nucleocapsid Proteins
references: J Gen Virol. 1993 Sep;74 ( Pt 9):1795-804
references: 8397280
references: Virology. 1994 Feb;198(2):466-76
references: 8291230
references: Vet Microbiol. 2010 Aug 26;144(3-4):293-302
references: 20299165
references: Vaccine. 1992;10(2):119-24
references: 1311490
references: Virus Res. 2010 May;149(2):175-82
references: 20132850
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Arch Virol. 1987;97(1-2):123-30
references: 2825619
references: Virus Genes. 2007 Oct;35(2):321-32
references: 17436070
references: Virus Genes. 2005 Oct;31(2):153-62
references: 16025240
references: J Virol. 2005 Jan;79(2):1164-79
references: 15613344
references: J Vet Med Sci. 1999 Jul;61(7):827-30
references: 10458108
references: Arch Virol. 1978;58(3):243-7
references: 83132
references: Clin Diagn Lab Immunol. 1998 May;5(3):412-4
references: 9606002