title: Natural hybridization between Paragonimus bangkokensis and Paragonimus harinasutai.
abstract: Paragonimus bangkokensis and Paragonimus harinasutai, which are morphologically distinguishable species, often co-infect in the same crab intermediate hosts. Recent molecular phylogenetic analyses revealed that these two species are genetically close to each other and are considered as the sister species. While we have been studying Paragonimus adult worms obtained from the lungs of a cat experimentally infected with Paragonimus metacercariae which were morphologically identified as P. harinasutai collected from central Viet Nam, one out of 6 adult worms has grouped cuticular spines, which is a feature of P. bangkokensis. By molecular analyses, the CO1 sequence of this specimen was identical with that of P. bangkokensis, but the ITS2 and the D2 region of 28S rDNA sequences showed a two peak pattern. Then, PCR products of the ITS2 and the D2 region of 28S rDNA sequences were ligated to TOPO vector and subcloned to determine the heterozygosity. Two types of sequences were obtained from each ITS2 and D2 region of 28S; one was identical with P. harinasutai and the other with P. bangkokensis. Taking all these morphological and molecular data together, we identified this adult worm as a hybrid specimen of P. bangkokensis and P. harinasutai.
authors: Department of Parasitology, Institute of Ecology and Biological Resources, Viet Nam Academy of Science and Technology, Hanoi, Viet Nam. pndoanh@iebr.ac.vn
authors: Pham Ngoc
authors: PN
authors: Doanh
authors: Zhihong
authors: Z
authors: Guo
authors: Nariaki
authors: N
authors: Nonaka
authors: Yoichiro
authors: Y
authors: Horii
authors: Yukifumi
authors: Y
authors: Nawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000469:parasitology*; D002415:Cats; D060085:Coinfection; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005243:Feces / Q000469:parasitology; D050259:Genes, Mitochondrial / Q000235:genetics; D005838:Genotype; D006579:Heterozygote; D006824:Hybridization, Genetic; D008168:Lung / Q000469:parasitology; D058488:Metacercariae; D008969:Molecular Sequence Data; D010237:Paragonimiasis / Q000469:parasitology / Q000662:veterinary*; D010238:Paragonimus / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D018510:RNA, Helminth / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014744:Vietnam / Q000453:epidemiology
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D018510:RNA, Helminth; D012339:RNA, Ribosomal, 28S; D003576:Electron Transport Complex IV