title: Sterol regulatory element binding transcription factor 1 (SREBF1) polymorphism and milk fatty acid composition.
abstract: Milk is known to contain high concentrations of saturated fatty acids-such as palmitic (16:0), myristic (14:0), and lauric (12:0) acids-that can raise plasma cholesterol in humans, making their presence in milk undesirable. The main objective of our candidate gene study was to develop genetic markers that can be used to improve the healthfulness of bovine milk. The sterol regulatory element binding transcription factor 1 (SREBF1) known to regulate the transcription of lipogenic genes together with SREBF chaperone and insulin induced gene 1 were the candidate genes. The results showed significant association of the overall SREBF1 haplotypes with milk production and variations in lauric (12:0) and myristic (14:0) acid concentrations in milk. Haplotype H1 of SREBF1 was the most desirable to improve milk healthfulness because it was significantly associated with lower lauric (12:0) and myristic (14:0) acid concentrations compared with haplotype H3 of SREBF1, and lower lauric acid (12:0) concentration compared with haplotype H2 of SREBF1. Haplotype H1 of SREBF1, however, was significantly associated with lower milk production compared with haplotype H3 of SREBF1. We did not detect any significant associations between genetic polymorphisms in insulin induced gene 1 (INSIG1) and SREBF chaperone and milk fatty acid composition. In conclusion, genetic polymorphisms in SREBF1 can be used to develop genetic tools for the selection of animals producing milk with healthier fatty acid composition.
authors: Department of Animal Science, Iowa State University, Ames 50011.
authors: R A
authors: RA
authors: Nafikov
authors: Department of Animal Science, Iowa State University, Ames 50011.
authors: J P
authors: JP
authors: Schoonmaker
authors: Department of Animal Science, Iowa State University, Ames 50011.
authors: K T
authors: KT
authors: Korn
authors: Department of Animal Science, Iowa State University, Ames 50011.
authors: K
authors: K
authors: Noack
authors: Department of Animal Science, Iowa State University, Ames 50011.
authors: D J
authors: DJ
authors: Garrick
authors: Department of Statistics, Iowa State University, Ames 50011.
authors: K J
authors: KJ
authors: Koehler
authors: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors: J
authors: J
authors: Minick-Bormann
authors: Department of Animal Science, Iowa State University, Ames 50011.
authors: J M
authors: JM
authors: Reecy
authors: Department of Animal Science, Iowa State University, Ames 50011.
authors: D E
authors: DE
authors: Spurlock
authors: Department of Animal Science, Iowa State University, Ames 50011. Electronic address: dcbeitz@iastate.edu.
authors: D C
authors: DC
authors: Beitz
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D006239:Haplotypes; D006293:Health Promotion; D007774:Lactation / Q000235:genetics; D007850:Lauric Acids / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D019814:Myristic Acid / Q000032:analysis; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012641:Selection, Genetic; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D005819:Genetic Markers; D007850:Lauric Acids; D051780:Sterol Regulatory Element Binding Protein 1; D019814:Myristic Acid; C030358:lauric acid