title: Barcode DNA high-resolution melting (Bar-HRM) analysis as a novel close-tubed and accurate tool for olive oil forensic use.
abstract: BACKGROUND
  The adulteration of high-priced olive oil with low-cost oils and the fraudulent labelling of oil products make the identification and traceability of vegetable oil species in the food chain very important. This paper describes a high-resolution melting analysis-based method using chloroplast barcoding regions as target (Bar-HRM) to obtain barcoding information for the major vegetable oil species and to quantitatively identify the botanical origin of plant oils. The detection of adulteration of olive oil with canola oil was used as a case study.
  
  
  RESULTS
  The proposed method was capable of distinguishing among different vegetable oil species and detecting a level of 1% (w/w) of canola oil in olive oil.
  
  
  CONCLUSIONS
  Bar-HRM analysis is a more accurate, faster and less costly alternative method to authenticate vegetable oils, including olive oil, and to detect mixtures of oils.
authors: Institute of Applied Bioscences, CERTH, 6th km Charilaou-Thermis Road, Thermi, Thessaloniki, 57001, Greece.
authors: Ioannis
authors: I
authors: Ganopoulos
authors: Christos
authors: C
authors: Bazakos
authors: Panagiotis
authors: P
authors: Madesis
authors: Panagiotis
authors: P
authors: Kalaitzis
authors: Athanasios
authors: A
authors: Tsaftaris
mesh_terms: D029688:Brassica napus / Q000737:chemistry / Q000378:metabolism; D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant / Q000032:analysis* / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000032:analysis / Q000737:chemistry; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D005515:Food Labeling; D053593:Forensic Toxicology / Q000379:methods; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism; D006115:Greece; D057230:Limit of Detection; D009691:Nucleic Acid Denaturation; D031658:Olea / Q000737:chemistry* / Q000378:metabolism; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D000074262:Rapeseed Oil; D060888:Real-Time Polymerase Chain Reaction; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D005229:Fatty Acids, Monounsaturated; D000069463:Olive Oil; D010938:Plant Oils; D010940:Plant Proteins; D000074262:Rapeseed Oil; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase