title: GmGBP1, a homolog of human ski interacting protein in soybean, regulates flowering and stress tolerance in Arabidopsis.
abstract: BACKGROUND
  SKIP is a transcription cofactor in many eukaryotes. It can regulate plant stress tolerance in rice and Arabidopsis. But the homolog of SKIP protein in soybean has been not reported up to now.
  
  
  RESULTS
  In this study, the expression patterns of soybean GAMYB binding protein gene (GmGBP1) encoding a homolog of SKIP protein were analyzed in soybean under abiotic stresses and different day lengths. The expression of GmGBP1 was induced by polyethyleneglycol 6000, NaCl, gibberellin, abscisic acid and heat stress. GmGBP1 had transcriptional activity in C-terminal. GmGBP1 could interact with R2R3 domain of GmGAMYB1 in SKIP domain to take part in gibberellin flowering pathway. In long-day (16 h-light) condition, transgenic Arabidopsis with the ectopic overexpression of GmGBP1 exhibited earlier flowering and less number of rosette leaves; Suppression of AtSKIP in Arabidopsis resulted in growth arrest, flowering delay and down-regulation of many flowering-related genes (CONSTANS, FLOWERING LOCUS T, LEAFY); Arabidopsis myb33 mutant plants with ectopic overexpression of GmGBP1 showed the same flowering phenotype with wild type. In short-day (8 h-light) condition, transgenic Arabidopsis plants with GmGBP1 flowered later and showed a higher level of FLOWERING LOCUS C compared with wild type. When treated with abiotic stresses, transgenic Arabidopsis with the ectopic overexpression of GmGBP1 enhanced the tolerances to heat and drought stresses but reduced the tolerance to high salinity, and affected the expressions of several stress-related genes.
  
  
  CONCLUSIONS
  In Arabidopsis, GmGBP1 might positively regulate the flowering time by affecting CONSTANS, FLOWERING LOCUS T, LEAFY and GAMYB directly or indirectly in photoperiodic and gibberellin pathways in LDs, but GmGBP1 might represse flowering by affecting FLOWERING LOCUS C and SHORT VEGETATIVE PHASE in autonomous pathway in SDs. GmGBP1 might regulate the activity of ROS-eliminating to improve the resistance to heat and drought but reduce the high-salinity tolerance.
authors: Key Laboratory of Soybean Biology in Chinese Education Ministry, College of Agronomy, Northeast Agricultural University, Harbin, 150030, China.
authors: Yanwei
authors: Y
authors: Zhang
authors: Lin
authors: L
authors: Zhao
authors: Haiyan
authors: H
authors: Li
authors: Yang
authors: Y
authors: Gao
authors: Yongguang
authors: Y
authors: Li
authors: Xiaoxia
authors: X
authors: Wu
authors: Weili
authors: W
authors: Teng
authors: Yingpeng
authors: Y
authors: Han
authors: Xue
authors: X
authors: Zhao
authors: Wenbin
authors: W
authors: Li
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D055864:Droughts; D035264:Flowers / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D030262:Soybean Proteins / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics / Q000378:metabolism*
chemical_list: D030262:Soybean Proteins
references: Crop Sci. 2002 Jan;42(1):271-277
references: 11756285
references: New Phytol. 2010 Jan;185(1):103-13
references: 19765229
references: Biochem Biophys Res Commun. 2010 Apr 16;394(4):1018-23
references: 20331973
references: J Exp Bot. 2010 May;61(9):2247-54
references: 20413527
references: Nature. 2003 Jun 19;423(6942):881-5
references: 12815435
references: Plant Cell. 2008 Nov;20(11):2960-71
references: 19011118
references: Trends Plant Sci. 2002 Sep;7(9):405-10
references: 12234732
references: Plant Cell. 2011 Mar;23(3):1138-52
references: 21398568
references: Plant J. 1999 Jan;17(1):1-9
references: 10069063
references: Plant J. 2003 Sep;35(5):613-23
references: 12940954
references: Plant Cell. 2005 Mar;17(3):705-21
references: 15722475
references: Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16216-21
references: 15534205
references: Planta. 2011 Nov;234(5):933-43
references: 21681526
references: Plant Cell. 2003 Jan;15(1):63-78
references: 12509522
references: Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6410-5
references: 19339499
references: Cell Mol Life Sci. 2004 Mar;61(6):629-40
references: 15052407
references: Plant Physiol. 2010 Nov;154(3):1220-31
references: 20864544
references: J Biochem. 2002 Oct;132(4):557-63
references: 12359070
references: Plant Cell. 2012 Aug;24(8):3278-95
references: 22942380
references: J Exp Bot. 2009;60(7):1979-89
references: 19264752
references: Chromosoma. 1992 Jun;101(8):517-25
references: 1424996
references: EMBO J. 2002 Aug 15;21(16):4327-37
references: 12169635
references: Curr Opin Plant Biol. 2003 Feb;6(1):13-9
references: 12495746
references: Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9254-9
references: 12084813
references: Bioessays. 2004 Apr;26(4):363-73
references: 15057934
references: Annu Rev Plant Biol. 2004;55:373-99
references: 15377225
references: Plant Cell. 2006 Sep;18(9):2172-81
references: 16920780
references: J Exp Bot. 2008;59(14):3821-9
references: 18931352
references: Curr Opin Plant Biol. 2008 Oct;11(5):486-94
references: 18653378
references: Mech Dev. 2002 Sep;117(1-2):151-62
references: 12204255
references: Plant Cell. 2002;14 Suppl:S111-30
references: 12045273
references: Plant Cell. 2008 Feb;20(2):292-306
references: 18296627
references: Planta. 2011 Mar;233(3):561-8
references: 21116647
references: Oncogene. 1998 Mar 26;16(12):1579-86
references: 9569025
references: Plant Physiol. 2001 Dec;127(4):1682-93
references: 11743113
references: J Exp Bot. 2012 Jun;63(10):3523-43
references: 22467407
references: Science. 2000 Jun 2;288(5471):1613-6
references: 10834834
references: Nature. 2000 Apr 20;404(6780):889-92
references: 10786797
references: RNA. 2003 Jan;9(1):138-50
references: 12554883