title: A simplified micropatterning method for straight-line neurite extension of cultured hippocampal neurons.
abstract: We report a simplified micropatterning method for the straight-line extension of the neurites of cultured neurons.  We prepared a poly-D-lysine (PDL)-patterned surface using a polydimethylsiloxane microfluidic stamp.  Hippocampal neurons were cultured on the PDL-bound substrate with the stamp removed, allowing for conventional cell seeding and detailed optical observation without fluorescent label.  Cultured neurons elongated neurites along straight lines at the single-cell level and displayed spontaneous firing as detected by time-lapse imaging and Ca(2+) imaging.
authors: Graduate School of Bionics, Tokyo University of Technology, Tokyo, Japan. isuzuki@stf.teu.ac.jp
authors: Ikuro
authors: I
authors: Suzuki
authors: Kosuke
authors: K
authors: Nakamura
authors: Aoi
authors: A
authors: Odawara
authors: Amani
authors: A
authors: Alhebshi
authors: Masao
authors: M
authors: Gotoh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006624:Hippocampus / Q000166:cytology*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D016501:Neurites / Q000378:metabolism*; D011107:Polylysine / Q000737:chemistry; D011327:Printing / Q000295:instrumentation / Q000379:methods*; D051381:Rats
chemical_list: D004129:Dimethylpolysiloxanes; D011107:Polylysine; C013830:baysilon