title: A novel EPS-producing strain of Bacillus licheniformis isolated from a shallow vent off Panarea Island (Italy).
abstract: A haloalkaliphilic, thermophilic Bacillus strain (T14), isolated from a shallow hydrothermal vent of Panarea Island (Italy), produced a new exopolysaccharide (EPS). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T14 was highly related (99 % similarity) to Bacillus licheniformis DSM 13(T) and Bacillus sonorensis DSM 13779(T). Further DNA-DNA hybridization analysis revealed 79.40 % similarity with B. licheniformis DSM 13(T) and 39.12 % with B. sonorensis DSM 13779(T). Sucrose (5 %) was the most efficient carbon source for growth and EPS production. The highest EPS production (366 mg l(-1)) was yielded in fermenter culture at 300 rpm after 48 h of incubation. The purified fraction EPS1 contained fructose/fucose/glucose/galactosamine/mannose in a relative proportion of 1.0:0.75:0.28:tr:tr and possessed a molecular weight of 1,000 kDa displaying a trisaccharide unit constituted by sugars with a β-manno-pyranosidic configuration. Screening for biological activity showed anti-cytotoxic effect of EPS1 against Avarol in brine shrimp test, indicating a potential use in the development of novel drugs.
authors: Department of Biological and Environmental Sciences, University of Messina, V.le F. Stagno d'Alcontres 31, 98166 Messina, Italy.
authors: Antonio
authors: A
authors: Spanò
authors: Concetta
authors: C
authors: Gugliandolo
authors: Valeria
authors: V
authors: Lentini
authors: Teresa L
authors: TL
authors: Maugeri
authors: Gianluca
authors: G
authors: Anzelmo
authors: Annarita
authors: A
authors: Poli
authors: Barbara
authors: B
authors: Nicolaus
mesh_terms: D000818:Animals; D001156:Artemia; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060086:Hydrothermal Vents / Q000382:microbiology*; D062312:Islands; D007558:Italy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013395:Sucrose / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D013395:Sucrose
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