title: Determination of the optimal time for radioiodine therapy in anaplastic             thyroid carcinoma using the adenovirus-mediated transfer of sodium iodide symporter             gene.
abstract: Gene therapy using human sodium iodide symporter (hNIS) and radioiodine             has been considered promising in a variety of gene therapy trials. However, the             optimal timing of radioiodine application following hNIS gene transfer remains             unknown. The present study aimed to investigate the serial expression of hNIS             following adenovirus-mediated hNIS gene transfer into anaplastic thyroid carcinoma             (ARO) to determine the optimal timing of radioiodine application. Recombinant             adenovirus encoding the hNIS gene (rAd-hNIS) was generated using a homologous             recombination reaction. The iodine uptake of rAd-hNIS‑transfected ARO cells gradually             increased until 120 min post‑125I application but the fold increase, reflecting             the relative uptake of rAd-hNIS‑transfected compared to non‑transfected ARO cells,             reached plateau at 60 min post‑125I application. For the in vivo analysis, rAd-hNIS             was injected intratumorally into ARO cell xenografts in the thighs of nude mice             (n=12). Two, 3, 4 and 6 days after rAd-hNIS injection, γ‑scintigraphic images             were obtained 60 min following injection of 5.5 MBq of 131I intraperitoneally.             Treated/non-treated (T/NT) xenograft count ratios were the highest at day 2 post‑rAd-hNIS             injection (2.85±0.61), and gradually decreased thereafter (2.54±0.65, 2.31±0.42             and 2.18±0.90 at days 3, 4 and 6 post‑rAd-hNIS injection, respectively). Real‑time             polymerase chain reaction (RT-PCR) and immunohistochemical staining demonstrated             that hNIS expression was the highest at day 2 following rAd-hNIS injection. In             conclusion, the optimal timing for radioiodine administration is day 2 after adenovirus-mediated             hNIS gene transfer into anaplastic thyroid carcinoma.
authors: Department of Nuclear Medicine, Konkuk University School of Medicine,                     Chungju, Republic of Korea.
authors: Young
authors: Y
authors: So
authors: Yong Jin
authors: YJ
authors: Lee
authors: Won Woo
authors: WW
authors: Lee
authors: June-Key
authors: JK
authors: Chung
mesh_terms: D000256:Adenoviridae; D000818:Animals; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D015316:Genetic Therapy; D006801:Humans; D007457:Iodine Radioisotopes / Q000008:administration & dosage; D051379:Mice; D008819:Mice, Nude; D012974:Sodium Iodide / Q000008:administration & dosage*; D027981:Symporters / Q000235:genetics*; D065646:Thyroid Carcinoma, Anaplastic; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays; D000099209:Sodium-Iodide Symporters
chemical_list: D007457:Iodine Radioisotopes; D012974:Sodium Iodide; D027981:Symporters; D000099209:Sodium-Iodide Symporters