title: Low prevalence of Leishmania donovani infection among the blood donors in kala-azar endemic areas of Bangladesh.
abstract: BACKGROUND
  Visceral leishmaniasis (VL) is a major public health problem in Bangladesh with the highest disease burden in the Mymensingh District. The disease is transmitted by sand fly bites, but it may also be transmitted through blood transfusions. No information is available about the prevalence of Leishmania infection among blood donors in Bangladesh; therefore we aimed to investigate this question.
  
  
  METHODS
  The study was carried out in the Blood Transfusion Department of Mymensingh Medical College Hospital. One thousand one hundred and ninety five adult healthy blood donors attending in this department were enrolled in the study from August 2010 to April 2011. After obtaining written consent, socio-demographic data and a detailed health history were collected. The medical officer in the unit performed a complete physical examination to exclude any acute or chronic diseases, which was followed by sero-diagnosis for exposure to Leishmania by rK39 strip test using finger prick blood. Blood donors with a positive rK39 strip test underwent a PCR test for detection of leishmania DNA in their peripheral blood buffy coat.
  
  
  RESULTS
  Eighty two percent of enrolled blood donors were male (n=985) and 18% (n=210) were female. The mean age of blood donors was 27 years (SD, 7.95 years). The majority of donors were literate and had mid-to-higher socioeconomic condition reflected by household conditions reported by the subject. Only 2.6% had a family member with VL in the past. Three blood donors were positive for leishmania infection by rK39 strip test (0.3%, 95%CI, 0.05%-0.73%). None of these 3 had active leishmania infection as demonstrated by PCR analysis. During six months of follow up, neither rK39 positive (n=3) nor rK39 negative (n=1192) donors developed VL.
  
  
  CONCLUSIONS
  The prevalence of Leishmania donovani infection among blood donors attending the Blood Transfusion Department of Mymensingh Medical College Hospital was very low. Therefore the chance for transmission of VL through blood transfusion is negligible. We believe that the National VL Elimination Program does not need set up routine screening for Leishmania donovani infection in blood transfusion departments located in VL endemic areas of Bangladesh.
authors: Centre for Communicable Disease, icddr,b, Mohakhali, Dhaka 1212, Bangladesh.
authors: M Mamun
authors: MM
authors: Huda
authors: Shikha
authors: S
authors: Rudra
authors: Debashis
authors: D
authors: Ghosh
authors: Khondaker Rifat Hasan
authors: KR
authors: Bhaskar
authors: Rajib
authors: R
authors: Chowdhury
authors: Aditya Prasad
authors: AP
authors: Dash
authors: Sujit Kumar
authors: SK
authors: Bhattacharya
authors: Rashidul
authors: R
authors: Haque
authors: Dinesh
authors: D
authors: Mondal
mesh_terms: D000328:Adult; D001459:Bangladesh / Q000453:epidemiology; D001782:Blood Donors; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007893:Leishmania donovani / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology*; D008297:Male; D015995:Prevalence; D055815:Young Adult
references: Trop Med Int Health. 2007 Feb;12(2):284-9
references: 17300637
references: Rev Soc Bras Med Trop. 2006 Sep-Oct;39(5):421-7
references: 17160317
references: Transfusion. 1996 Sep;36(9):848-9
references: 8823466
references: J Clin Microbiol. 1999 Jun;37(6):1953-7
references: 10325353
references: Am J Trop Med Hyg. 2000 Jan;62(1):128-31
references: 10761737
references: Am J Trop Med Hyg. 2004 Jan;70(1):72-7
references: 14971701
references: J Vector Borne Dis. 2008 Jun;45(2):105-11
references: 18592839
references: Trans R Soc Trop Med Hyg. 2002 Apr;96 Suppl 1:S185-9
references: 12055836
references: BMJ. 2006 Oct 7;333(7571):723
references: 16882683
references: Indian J Med Microbiol. 2006 Jul;24(3):165-70
references: 16912434
references: Indian J Med Res. 2006 Mar;123(3):275-88
references: 16778310
references: Am J Trop Med Hyg. 1997 Aug;57(2):168-71
references: 9288810
references: Transfus Med. 2004 Aug;14(4):319-21
references: 15285729
references: Acta Trop. 2010 Jan;113(1):52-5
references: 19769932
references: Trans R Soc Trop Med Hyg. 2004 Feb;98(2):102-10
references: 14964810