title: Association of IL27 gene polymorphisms and HBV-related hepatocellular carcinoma risk in a Chinese population.
abstract: OBJECTIVE
  Hepatocellular carcinoma (HCC) is multifactorial, and the genetic background may be a crucial etiologic factor. Interleukin-27 (IL-27) is a novel IL-12 family member which plays an important role in antitumor immunity. Mutations in the IL27 gene may lead to altered cytokine production and/or activity and thus modulate individual's susceptibility to HCC. In this study, we investigated the association between IL27 gene polymorphisms and HBV-related diseases risk in a Chinese population.
  
  
  METHODS
  Studied subjects were divided into four groups: 112 patients with chronic hepatitis B (CHB), 65 patients with hepatitis B virus (HBV)-related liver cirrhosis (LC), 107 patients with HBV-related HCC, and 105 healthy controls. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy and polymerase chain reaction-sequence specific primer (PCR-SSP) strategy were used to detect IL27 gene -964A/G and 2905T/G polymorphisms, respectively. DNA sequencing was used to validate genotype results.
  
  
  RESULTS
  There were no significant differences in the genotype and allele frequencies of IL27 gene polymorphisms between the groups of patients and healthy controls. Furthermore, no association was found between the distributions of the haplotypes and HCC risk.
  
  
  CONCLUSIONS
  These findings indicate that the genetic variants in IL27 gene may not contribute to HCC development. Further studies with large sample size should be conducted to validate this association.
authors: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors: Qiliu
authors: Q
authors: Peng
authors: Xue
authors: X
authors: Qin
authors: Yani
authors: Y
authors: He
authors: Zhiping
authors: Z
authors: Chen
authors: Yan
authors: Y
authors: Deng
authors: Taijie
authors: T
authors: Li
authors: Li
authors: L
authors: Xie
authors: Jinmin
authors: J
authors: Zhao
authors: Shan
authors: S
authors: Li
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000235:genetics*; D016009:Chi-Square Distribution; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D064094:Interleukin-27 / Q000235:genetics*; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012189:Retrospective Studies
chemical_list: D064094:Interleukin-27