title: Theoretical and experimental dissection of DNA loop-mediated repression.
abstract: Transcriptional networks across all domains of life feature a wide range of regulatory architectures. Theoretical models now make clear predictions about how key parameters describing those architectures modulate gene expression, and the ability to construct genetic circuits with tunable parameters enables precise tests of such models. We dissect gene regulation through DNA looping by tuning network parameters such as repressor copy number, DNA binding strengths, and loop length in both thermodynamic models and experiments. Our results help clarify the short-length mechanical properties of DNA.
authors: Department of Applied Physics, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA.
authors: James Q
authors: JQ
authors: Boedicker
authors: Hernan G
authors: HG
authors: Garcia
authors: Rob
authors: R
authors: Phillips
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D013329:Structure-Activity Relationship; D013816:Thermodynamics
chemical_list: D004247:DNA
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