title: Phenotypic redifferentiation and cell cluster formation of cultured human articular chondrocytes in a three-dimensional oriented gelatin scaffold in the presence of PGE2--first results of a pilot study.
abstract: Modern tissue engineering strategies comprise three elemental parameters: cells, scaffolds and growth factors. Articular cartilage represents a highly specialized tissue which allows frictionless gliding of corresponding articulating surfaces. As the regenerative potential of cartilage is low, tissue engineering-based strategies for cartilage regeneration represent a huge challenge. Prostaglandins function as regulators in cartilage development and metabolism, especially in growth plate chondrocytes. In this study, it was analyzed if prostaglandin E2 (PGE2 ) has an effect on the phenotypic differentiation of human chondrocytes cultured in a three-dimensional (3D) gelatin-based scaffold made by directional freezing and subsequent freeze-drying. As a result, it was clearly demonstrated that low doses of PGE2 revealed beneficial effects on the phenotypic differentiation and collagen II expression of human articular chondrocytes in this 3D cell culture system. In conclusion, PGE2 is an interesting candidate for tissue engineering applications since it represents an already well-studied molecule which is available in pharmaceutical quality.
authors: REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Mainz, Germany. brochhausen@pathologie.klinik.uni-mainz.de
authors: Christoph
authors: C
authors: Brochhausen
authors: Natalia
authors: N
authors: Sánchez
authors: Sven
authors: S
authors: Halstenberg
authors: Rolf
authors: R
authors: Zehbe
authors: Bernhard
authors: B
authors: Watzer
authors: Volker H
authors: VH
authors: Schmitt
authors: Alexander
authors: A
authors: Hofmann
authors: Andrea
authors: A
authors: Meurer
authors: Ron E
authors: RE
authors: Unger
authors: Charles James
authors: CJ
authors: Kirkpatrick
mesh_terms: D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics; D015232:Dinoprostone / Q000008:administration & dosage* / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry*; D005786:Gene Expression Regulation; D006801:Humans; D010865:Pilot Projects; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II; D005780:Gelatin; D015232:Dinoprostone