title: Assessment of fine needle aspiration feasibility and specimen adequacy for molecular diagnostics of benign vocal fold lesions.
abstract: OBJECTIVE
  The use of molecular testing is becoming more significant for the diagnosis and classification of disease. The application of fine-needle aspiration (FNA) biopsy as the means of sampling lesions in union with molecular testing could be a powerful combination in laryngology. The objectives of this study were to investigate 1) if FNA was feasible to sample benign vocal fold lesions; 2) if FNA samples provided sufficient RNA quality for molecular analysis; and 3) if gene expression of FNA samples matched paired surgical excised specimens.
  
  
  METHODS
  Prospective cross-sectional.
  
  
  METHODS
  Fifteen vocal fold specimens were obtained from adult patients undergoing routine surgical removal for benign vocal fold lesions using FNA and surgical excision. Comparisons were made between FNA and excision biopsies for RNA quality. Correlative analysis was completed for RNA expression of nine genes, including decorin (DCN), connective tissue growth factor (CTGF), vascular endothelial growth factor (VEGF), collagen type VI alpha 3 (COL6A3), superoxide dismutase 1 (SOD1), glutathione S-transferase (GST2), collagen type I alpha 2 (COL1A2), ATP binding cassette (ABC), and procollagen I alpha 1 (COL1A1).
  
  
  RESULTS
  FNA and excision samples demonstrated similar RNA quality (P > 0.05). Per gene expression, four out of nine genes were moderately correlated between the paired samples (P < 0.05).
  
  
  CONCLUSIONS
  FNA of the vocal fold lamina propria is technically feasible to perform. Further improvement in the FNA technology is desirable to optimize RNA quality for reliable gene expression analysis.
authors: Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin 53705-2275, USA.
authors: Nicole Y K
authors: NY
authors: Li
authors: Seth
authors: S
authors: Dailey
authors: Susan L
authors: SL
authors: Thibeault
mesh_terms: D000328:Adult; D044963:Biopsy, Fine-Needle; D003430:Cross-Sectional Studies; D003560:Cysts / Q000235:genetics / Q000473:pathology; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007818:Laryngeal Diseases / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D057089:Pathology, Molecular / Q000592:standards; D011127:Polyps / Q000235:genetics / Q000473:pathology; D011446:Prospective Studies; D014827:Vocal Cords / Q000473:pathology*; D055815:Young Adult
references: J Voice. 2006 Mar;20(1):110-20
references: 15964741
references: Nat Med. 2002 May;8(5):451-3
references: 11984584
references: Nature. 2000 Feb 3;403(6769):503-11
references: 10676951
references: Nat Med. 2002 May;8(5):500-8
references: 11984595
references: Nat Med. 2002 Jan;8(1):13-4
references: 11786894
references: Nature. 2000 Aug 17;406(6797):747-52
references: 10963602
references: J Voice. 2007 Sep;21(5):617-22
references: 16806815
references: Ann Otol Rhinol Laryngol. 2006 Oct;115(10):764-8
references: 17076099
references: Nature. 2000 Aug 3;406(6795):536-40
references: 10952317
references: Breast Cancer Res. 2002;4(3):R3
references: 12052255
references: J Otolaryngol. 2002 Dec;31(6):376-80
references: 12593551
references: Science. 1999 Oct 15;286(5439):531-7
references: 10521349
references: Ann Otol Rhinol Laryngol. 2006 Sep;115(9):703-14
references: 17044544
references: J Indian Med Assoc. 2011 Apr;109(4):258-61
references: 22187797
references: Lab Invest. 2005 Aug;85(8):1040-50
references: 15951835
references: Breast J. 2012 Jan-Feb;18(1):3-7
references: 22098412
references: Diagn Cytopathol. 2010 Jan;38(1):65-80
references: 19582815
references: J Voice. 2004 Mar;18(1):125-9
references: 15070232
references: World J Surg. 2012 Jan;36(1):69-74
references: 22057754
references: Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9991-6
references: 12900505
references: Laryngoscope. 2011 Mar;121(3):548-54
references: 21305549
references: Am J Clin Pathol. 2011 Jul;136(1):45-59
references: 21685031
references: Nat Med. 2002 Jan;8(1):68-74
references: 11786909
references: Pathology (Phila). 1996;4(2):409-38
references: 9238365
references: Clin Cancer Res. 2003 Dec 1;9(16 Pt 1):5980-7
references: 14676123
references: Laryngoscope. 2012 Jun;122(6):1335-41
references: 22522621
references: Mol Aspects Med. 2006 Apr-Jun;27(2-3):126-39
references: 16469371
references: Cancer Res. 2002 Jul 1;62(13):3581-6
references: 12097256
references: J Mol Diagn. 2010 Sep;12(5):566-75
references: 20709792
references: J Speech Lang Hear Res. 2003 Apr;46(2):491-502
references: 14700388