title: Hypoxia-ischemia alters nucleotide and nucleoside catabolism and Na+,K+-ATPase activity in the cerebral cortex of newborn rats.
abstract: It is well known that the levels of adenosine in the brain increase dramatically during cerebral hypoxic-ischemic (HI) insults. Its levels are tightly regulated by physiological and pathophysiological changes that occur during the injury acute phase. The aim of the present study was to examine the effects of the neonatal HI event on cytosolic and ecto-enzymes of purinergic system--NTPDase, 5'-nucleotidase (5'-NT) and adenosine deaminase (ADA)--in cerebral cortex of rats immediately post insult. Furthermore, the Na(+)/K(+)-ATPase activity, adenosine kinase (ADK) expression and thiobarbituric acid reactive species (TBARS) levels were assessed. Immediately after the HI event the cytosolic NTPDase and 5'-NT activities were increased in the cerebral cortex. In synaptosomes there was an increase in the ecto-ADA activity while the Na(+)/K(+) ATPase activity presented a decrease. The difference between ATP, ADP, AMP and adenosine degradation in synaptosomal and cytosolic fractions could indicate that NTPDase, 5'-NT and ADA were differently affected after insult. Interestingly, no alterations in the ADK expression were observed. Furthermore, the Na(+)/K(+)-ATPase activity was correlated negatively with the cytosolic NTPDase activity and TBARS content. The increased hydrolysis of nucleotides ATP, ADP and AMP in the cytosol could contribute to increased adenosine levels, which could be related to a possible innate neuroprotective mechanism aiming at potentiating the ambient levels of adenosine. Together, these results may help the understanding of the mechanism by which adenosine is produced following neonatal HI injury, therefore highlighting putative therapeutical targets to minimize ischemic injury and enhance recovery.
authors: Department of Chemistry, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil. victor.capi@yahoo.com.br
authors: Victor Camera
authors: VC
authors: Pimentel
authors: Daniela
authors: D
authors: Zanini
authors: Andréia Machado
authors: AM
authors: Cardoso
authors: Roberta
authors: R
authors: Schmatz
authors: Margarete Dulce
authors: MD
authors: Bagatini
authors: Jessié Martins
authors: JM
authors: Gutierres
authors: Fabiano
authors: F
authors: Carvalho
authors: Jéssica Lopes
authors: JL
authors: Gomes
authors: Maribel
authors: M
authors: Rubin
authors: Vera Maria
authors: VM
authors: Morsch
authors: Maria Beatriz
authors: MB
authors: Moretto
authors: Mariana
authors: M
authors: Colino-Oliveira
authors: Ana Maria
authors: AM
authors: Sebastião
authors: Maria Rosa Chitolina
authors: MR
authors: Schetinger
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000248:Adenosine Kinase / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000503:physiopathology*; D008297:Male; D043583:Nucleoside-Triphosphatase / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D000248:Adenosine Kinase; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D011755:Pyrophosphatases; D043583:Nucleoside-Triphosphatase; C021895:nucleoside triphosphate pyrophosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D000241:Adenosine
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