title: Increased neuronal apoptosis in medial prefrontal cortex is accompanied with changes of Bcl-2 and Bax in a rat model of post-traumatic stress disorder.
abstract: Post-traumatic stress disorder (PTSD) is an anxiety disorder caused by traumatic experience, which affects a patient's quality of life and social stability. The objective of this study was to determine the apoptosis-related genes B-cell lymphoma 2 (Bcl-2) and BCL2-associated X (Bax) expressions and medial prefrontal cortex (mPFC) neuronal apoptosis after PTSD in rat model and therefore to provide experimental evidence to reveal PTSD pathogenesis. The single-prolonged stress (SPS) method was used to set up the rat PTSD models. Chemiluminescence was used to determine serum corticosterone levels. Neuronal apoptosis was detected using transmission electron microscopy, Hoechst staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling staining. Immunohistochemistry, immunofluorescence, RT-PCR, and Western blot were used to detect the expressions of Bcl-2 and Bax protein in mPFC. Our results showed an increased mPFC neuronal apoptosis after SPS stimulation. The number of apoptotic cells peaked on day 7. The expressions of Bcl-2 and Bax peaked on days 4 and 7. The Bcl-2/Bax ratio elevated on days 1 and 4 but decreased markedly on day 7. These results indicated that SPS stimulation increased the number of apoptotic neurons, up-regulated the expressions of Bcl-2 and Bax, and altered the Bcl-2/Bax ratio in the mPFC of PTSD rats.
authors: Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, No. 92 Beima Road, Heping District, Shenyang, 110001, Liaoning Province, China.
authors: Yana
authors: Y
authors: Li
authors: Fang
authors: F
authors: Han
authors: Yuxiu
authors: Y
authors: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis; D003345:Corticosterone / Q000097:blood; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D017397:Prefrontal Cortex / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013313:Stress Disorders, Post-Traumatic / Q000209:etiology / Q000378:metabolism*; D013315:Stress, Psychological / Q000150:complications; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D003345:Corticosterone
references: Br J Psychiatry. 2007 Nov;191:387-92
references: 17978317
references: Mol Med Rep. 2012 Nov;6(5):1140-4
references: 22895536
references: Leuk Res. 2007 Mar;31(3):277-86
references: 16911824
references: Biol Psychiatry. 2012 Jul 1;72(1):65-9
references: 22305287
references: Biol Psychiatry. 2010 Dec 1;68(11):1023-30
references: 20855060
references: Neurol Res. 2010 Jul;32(6):579-86
references: 20092675
references: Biol Psychiatry. 2003 Mar 15;53(6):463-73
references: 12644351
references: Int J Mol Med. 2009 Aug;24(2):227-31
references: 19578795
references: Ann N Y Acad Sci. 2006 Jul;1071:405-9
references: 16891587
references: Ann N Y Acad Sci. 2006 Jul;1071:67-79
references: 16891563
references: Clin Exp Metastasis. 2002;19(5):457-64
references: 12198774
references: Riv Psichiatr. 2012 Jan-Feb;47(1):59-64
references: 22358218
references: Tijdschr Psychiatr. 2009;51(8):595-602
references: 19658072
references: Am J Psychiatry. 2004 Jan;161(1):109-15
references: 14702258
references: Neuroimage. 2006 Jan 15;29(2):347-57
references: 16216534
references: Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1328-33
references: 12538851
references: Psychopharmacology (Berl). 2004 Mar;172(2):225-9
references: 14586539
references: PLoS One. 2010 Aug 05;5(8):e11971
references: 20700483
references: J Psychiatr Res. 2011 May;45(5):630-7
references: 21109253
references: Neuropsychologia. 2008 Sep;46(11):2836-44
references: 18597797
references: Arch Gen Psychiatry. 2005 Mar;62(3):273-81
references: 15753240
references: Trends Neurosci. 1995 Aug;18(8):355-8
references: 7482798
references: Neurobiol Dis. 2012 Aug;47(2):210-5
references: 22525571
references: Mol Med Rep. 2012 Aug;6(2):330-4
references: 22684778
references: Blood. 2002 Sep 1;100(5):1810-6
references: 12176904
references: J Biol Chem. 2002 Nov 15;277(46):44317-26
references: 12193597
references: Am J Psychiatry. 2007 Aug;164(8):1250-8
references: 17671289
references: Hum Pathol. 2002 Jul;33(7):686-92
references: 12196918
references: Br J Psychiatry. 2002 Aug;181:102-10
references: 12151279
references: Acta Psychiatr Scand. 2007 Dec;116(6):483-7
references: 17997727
references: Br J Cancer. 2002 Aug 27;87(5):555-61
references: 12189556
references: Biol Psychiatry. 2006 Aug 15;60(4):376-82
references: 16919525
references: J Psychiatr Res. 2010 Jan;44(1):48-55
references: 19586638
references: J Psychiatr Res. 2009 Sep;43(13):1078-85
references: 19380152
references: Psychol Med. 2011 Dec;41(12):2563-72
references: 21733221
references: Dialogues Clin Neurosci. 2011;13(3):263-78
references: 22034143
references: J Cutan Pathol. 2002 Feb;29(2):79-87
references: 12150137
references: Leukemia. 2002 Sep;16(9):1713-24
references: 12200686
references: Cent Eur Neurosurg. 2011 Aug;72(3):133-7
references: 20857373
references: Prostate. 2001 Sep 15;48(4):254-64
references: 11536305
references: Gynecol Obstet Invest. 2002;53(4):224-30
references: 12186988
references: J Chem Neuroanat. 2010 Nov;40(3):248-55
references: 20624456
references: Psychopharmacology (Berl). 2006 Dec;189(2):165-73
references: 17031709
references: J Mol Neurosci. 2013 Feb;49(2):360-8
references: 22930314
references: J Neuroendocrinol. 2011 Feb;23(2):107-17
references: 20973838
references: Zhonghua Yu Fang Yi Xue Za Zhi. 2011 Jun;45(6):531-6
references: 21914337
references: Cell Mol Neurobiol. 2011 Jan;31(1):37-43
references: 20803313
references: Neurochem Res. 2012 May;37(5):1043-9
references: 22294157
references: Arch Histol Cytol. 2002 Jun;65(2):145-57
references: 12164338
references: Psychoneuroendocrinology. 1997 Aug;22(6):443-53
references: 9364622
references: Photochem Photobiol. 2002 Aug;76(2):217-23
references: 12194220
references: Xenotransplantation. 2002 Sep;9(5):325-37
references: 12199864
references: Clin Immunol. 2002 Aug;104(2):151-60
references: 12165276
references: Physiol Behav. 2006 May 30;87(5):881-90
references: 16546226
references: J Psychiatry Neurosci. 2011 May;36(3):176-86
references: 21118656