title: Multilocus sequence typing and FlaA sequencing reveal the genetic stability of Campylobacter jejuni enrichment during coculture with Acanthamoeba polyphaga.
abstract: Low concentrations of Campylobacter jejuni cells in environmental samples make them difficult to study with conventional culture methods. Here, we show that enrichment by amoeba cocultures works well with low-concentration samples and that this method can be combined with molecular techniques without loss of genetic specificity.
authors: Centre for Ecology and Evolution in Microbial Model Systems (EEMiS), Linnaeus University, Kalmar, Sweden. petra.griekspoor@lnu.se
authors: Petra
authors: P
authors: Griekspoor
authors: Jenny
authors: J
authors: Olofsson
authors: Diana
authors: D
authors: Axelsson-Olsson
authors: Jonas
authors: J
authors: Waldenström
authors: Björn
authors: B
authors: Olsen
mesh_terms: D000048:Acanthamoeba / Q000254:growth & development*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D005408:Flagellin / Q000235:genetics*; D042822:Genomic Instability; D008828:Microbiological Techniques / Q000379:methods; D058885:Multilocus Sequence Typing
chemical_list: D005408:Flagellin; C067138:flaA protein, bacteria
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