title: Facilitated diffusion of acetonitrile revealed by quantitative breath analysis using extractive electrospray ionization mass spectrometry.
abstract: By using silver cations (Ag⁺) as the ionic reagent in reactive extractive electrospray ionization mass spectrometry (EESI-MS), the concentrations of acetonitrile in exhaled breath samples from the volunteers including active smokers, passive smokers, and non-smokers were quantitatively measured in vivo, without any sample pretreatment. A limit of detection (LOD) and relative standard deviation (RSD) were 0.16 ng/L and 3.5% (n = 8), respectively, for the acetonitrile signals in MS/MS experiments. Interestingly, the concentrations of acetonitrile in human breath continuously increased for 1-4 hours after the smoker finished smoking and then slowly decreased to the background level in 7 days. The experimental data of a large number of (> 165) samples indicated that the inhaled acetonitrile is excreted most likely by facilitated diffusion, instead of simple diffusion reported previously for other volatile compounds.
authors: National Institute of Metrology, Beijing 100013, P. R. China.
authors: Ming
authors: M
authors: Li
authors: Jianhua
authors: J
authors: Ding
authors: Haiwei
authors: H
authors: Gu
authors: Yan
authors: Y
authors: Zhang
authors: Susu
authors: S
authors: Pan
authors: Ning
authors: N
authors: Xu
authors: Huanwen
authors: H
authors: Chen
authors: Hongmei
authors: H
authors: Li
mesh_terms: D000097:Acetonitriles / Q000032:analysis*; D001944:Breath Tests; D002412:Cations / Q000737:chemistry; D004058:Diffusion; D006801:Humans; D012834:Silver / Q000737:chemistry; D012907:Smoking; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
chemical_list: D000097:Acetonitriles; D002412:Cations; D012834:Silver; C032159:acetonitrile
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