title: Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L.
abstract: We previously reported that a rare sugar D-allose, which is the D-glucose epimer at C3, inhibits the gibberellin-dependent responses such as elongation of the second leaf sheath and induction of α-amylase in embryo-less half seeds in rice (Fukumoto et al. 2011). D-Allose suppresses expressions of gibberellin-responsive genes downstream of SLR1 protein in the gibberellin-signaling through hexokinase (HXK)-dependent pathway. In this study, we discovered that D-allose induced expression of ABA-related genes including OsNCED1-3 and OsABA8ox1-3 in rice. Interestingly, D-allose also up-regulated expression of OsABF1, encoding a conserved bZIP transcription factor in ABA signaling, in rice. The D-allose-induced expression of OsABF1 was diminished by a hexokinase inhibitor, D-mannoheptulose (MNH). Consistently, D-allose also inhibited Arabidopsis growth, but failed to trigger growth retardation in the glucose-insensitive2 (gin2) mutant, which is a loss-of-function mutant of the glucose sensor AtHXK1. D-Allose activated AtABI5 expression in transgenic gin2 over-expressing wild-type AtHXK1 but not in gin2 over-expressing the catalytic mutant AtHXK1(S177A), indicating that the D-allose phosphorylation by HXK to D-allose 6-phosphate (A6P) is the first step for the up-regulation of AtABI5 gene expression as well as D-allose-induced growth inhibition. Moreover, overexpression of OsABF1 showed increased sensitivity to D-allose in rice. These findings indicated that the phosphorylation of D-allose at C6 by hexokinase is essential and OsABF1 is involved in the signal transduction for D-allose-induced growth inhibition.
authors: Faculty of Agriculture, Rare Sugar Research Center, and Gene Research Center, Kagawa University, Miki, Kagawa, 761-0795, Japan.
authors: Takeshi
authors: T
authors: Fukumoto
authors: Akihito
authors: A
authors: Kano
authors: Kouhei
authors: K
authors: Ohtani
authors: Megumi
authors: M
authors: Inoue
authors: Akihide
authors: A
authors: Yoshihara
authors: Ken
authors: K
authors: Izumori
authors: Shigeyuki
authors: S
authors: Tajima
authors: Yoshio
authors: Y
authors: Shigematsu
authors: Keiji
authors: K
authors: Tanaka
authors: Takeo
authors: T
authors: Ohkouchi
authors: Yutaka
authors: Y
authors: Ishida
authors: Yoko
authors: Y
authors: Nishizawa
authors: Yasuomi
authors: Y
authors: Tada
authors: Kazuya
authors: K
authors: Ichimura
authors: Kenji
authors: K
authors: Gomi
authors: Sang-Dong
authors: SD
authors: Yoo
authors: Jen
authors: J
authors: Sheen
authors: Kazuya
authors: K
authors: Akimitsu
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology*; D006593:Hexokinase / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; C002055:allose; D006593:Hexokinase; D005947:Glucose
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