title: A comparative study of the modulatory effects of (-)-cubebin on the mutagenicity/recombinogenicity induced by different chemical agents.
abstract: (-)-Cubebin (CUB) is a lignan isolated from dry seeds of Piper cubeba. We aimed to assess its genotoxic potential and influence on chromosomal damage (frequency of micronuclei - MN) induced by doxorubicin (DXR) in V79 cells and by urethane (URE) in somatic Drosophila melanogaster cells. Our findings indicate an absence of a CUB-mediated genotoxic effect at the concentrations tested. The results also revealed that CUB significantly reduced the frequency of MN induced by DXR, with a mean reduction of 63.88%. In a previous study, our research group demonstrated an absence of CUB-mediated mutagenic effects through the wing Somatic Mutation and Recombination Test (SMART) in Drosophila. In the present study, we used the standard and high bioactivation versions of the SMART to estimate the antigenotoxic effects of CUB associated with URE. At lower concentrations, the recombination level decreased, but at the highest concentration, the recombination level increased. Our data and previous studies suggest that CUB may act as a free radical scavenger at low concentrations, a pro-oxidant at higher concentrations when it interacts with the enzymatic system that catalyzes the metabolic detoxification of DXR or URE, and/or an inducer of recombinational DNA repair.
authors: Laboratory of Mutagenesis, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia (MG), Brazil.
authors: Alexandre Azenha Alves
authors: AA
authors: de Rezende
authors: Carla Carolina
authors: CC
authors: Munari
authors: Pollyanna Francielli
authors: PF
authors: de Oliveira
authors: Natália Helen
authors: NH
authors: Ferreira
authors: Denise Crispim
authors: DC
authors: Tavares
authors: Márcio Luis
authors: ML
authors: Andrade E Silva
authors: Karen Cristina Souza
authors: KC
authors: Rezende
authors: Mário Antônio
authors: MA
authors: Spanó
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D004331:Drosophila melanogaster; D017705:Lignans / Q000494:pharmacology*; D009153:Mutagens / Q000633:toxicity*; D011995:Recombination, Genetic / Q000187:drug effects*
chemical_list: D016587:Antimutagenic Agents; D017705:Lignans; D009153:Mutagens; C433065:cubebin