title: Weight loss improves the adipogenic capacity of human preadipocytes and modulates their secretory profile.
abstract: Calorie restriction-induced weight loss is accompanied by profound changes in adipose tissue characteristics. To determine the effect of weight loss on differentiation of preadipocytes and secretory capacity of in vitro differentiated adipocytes, we established cultures of these cells from paired subcutaneous adipose tissue biopsies obtained before and at the end of weight-reducing dietary intervention (DI) in 23 obese women. Based on lipid accumulation and the expression of differentiation markers, in vitro adipogenesis increased after weight loss and it was accompanied by enhanced expression of genes involved in de novo lipogenesis. This effect of weight loss was not driven by changes of peroxisome proliferator-activated receptor γ sensitivity to rosiglitazone. Weight loss also enhanced the expression of adiponectin and leptin while reducing that of monocyte chemoattractant protein 1 and interleukin-8 by cultured adipocytes. Thus, the weight-reducing (DI) increased adipogenic capacity of preadipocytes and shifted their secretion toward lower inflammatory profile. Reprogramming of preadipocytes could represent an adaptation to weight loss leading to partial restoration of preobese adipose tissue traits and thus contribute to the improvement of metabolic status. However, enhanced adipogenesis could also contribute to the unwanted weight regain after initial weight loss.
authors: Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic, and INSERM, Toulouse, France. lenka.rossmeislova@lf3.cuni.cz
authors: Lenka
authors: L
authors: Rossmeislová
authors: Lucia
authors: L
authors: Malisová
authors: Jana
authors: J
authors: Kracmerová
authors: Michaela
authors: M
authors: Tencerová
authors: Zuzana
authors: Z
authors: Kovácová
authors: Michal
authors: M
authors: Koc
authors: Michaela
authors: M
authors: Siklová-Vítková
authors: Nathalie
authors: N
authors: Viquerie
authors: Dominique
authors: D
authors: Langin
authors: Vladimír
authors: V
authors: Stich
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D052242:Adiponectin / Q000378:metabolism; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D009765:Obesity; D047495:PPAR gamma / Q000378:metabolism; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D015431:Weight Loss / Q000235:genetics / Q000502:physiology*
chemical_list: D052242:Adiponectin; D018932:Chemokine CCL2; D016207:Cytokines; D016209:Interleukin-8; D020738:Leptin; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone
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