title: The anticonvulant effect of cooling in comparison to α-lipoic acid: a neurochemical study.
abstract: Brain cooling has pronounced effects on seizures and epileptic activity. The aim of the present study is to evaluate the anticonvulsant effect of brain cooling on the oxidative stress and changes in Na(+), K(+)-ATPase and acetylcholinesterase (AchE) activities during status epilepticus induced by pilocarpine in the hippocampus of adult male rat in comparison with α-lipoic acid. Rats were divided into four groups: control, rats treated with pilocarpine for induction of status epilepticus, rats treated for 3 consecutive days with α-lipoic acid before pilocarpine and rats subjected to whole body cooling for 30 min before pilocarpine. The present findings indicated that pilocarine-induced status epilepticus was accompanied by a state of oxidative stress as clear from the significant increase in lipid peroxidation (MDA) and superoxide dismutase (SOD) and significant decrease in reduced glutathione and nitric oxide (NO) levels and the activities of catalase, AchE and Na(+), K(+)-ATPase. Pretreatment with α-lipoic acid ameliorated the state of oxidative stress and restored AchE to nearly control activity. However, Na(+), K(+)-ATPase activity showed a significant decrease. Rats exposed to cooling for 30 min before the induction of status epilepticus revealed significant increases in MDA and NO levels and SOD activity. AchE returned to control value while the significant decrease in Na(+), K(+)-ATPase persisted. The present data suggest that cooling may have an anticonvulsant effect which may be mediated by the elevated NO level. However, brain cooling may have drastic unwanted insults such as oxidative stress and the decrease in Na(+), K(+)-ATPase activity.
authors: Medical Division, Medical Physiology Department, National Research Center, El-Bohouth St., P.O. 12622, Giza, Egypt. yaserask@yahoo.com
authors: Yasser A
authors: YA
authors: Khadrawy
authors: Heba S
authors: HS
authors: Aboulezz
authors: Nawal A
authors: NA
authors: Ahmed
authors: Haitham S
authors: HS
authors: Mohammed
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D003080:Cold Temperature; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000494:pharmacology*
chemical_list: D000927:Anticonvulsants; D008063:Thioctic Acid
references: Exp Neurol. 1994 Sep;129(1):169-73
references: 7925839
references: Brain Res Dev Brain Res. 2002 Jul 30;137(1):43-54
references: 12128253
references: Epilepsia. 2002 Aug;43(8):932-5
references: 12181015
references: Z Naturforsch C J Biosci. 2000 Mar-Apr;55(3-4):271-7
references: 10817219
references: Cell Mol Neurobiol. 2010 May;30(4):569-75
references: 19941057
references: J Neurosci. 1990 Jan;10(1):311-6
references: 2405111
references: Anal Biochem. 1978 May;86(1):324-6
references: 655393
references: Pharmacol Biochem Behav. 1993 Jun;45(2):321-5
references: 8327537
references: Anesth Analg. 1987 Aug;66(8):751-5
references: 3111305
references: Pediatr Res. 1991 Feb;29(2):191-5
references: 2014158
references: J Neurosci. 1985 Mar;5(3):817-24
references: 3973697
references: J Neurochem. 1999 May;72(5):2047-52
references: 10217283
references: Neuron. 1992 Apr;8(4):653-62
references: 1314618
references: J Physiol. 2005 Aug 15;567(Pt 1):215-24
references: 15961429
references: Eur J Pharmacol. 1999 Feb 5;366(2-3):137-42
references: 10082193
references: Epilepsia. 1993 Jul-Aug;34(4):592-6
references: 8330566
references: Ann Neurol. 1992 Sep;32(3):297-311
references: 1384420
references: Seizure. 2008 Jan;17(1):76-83
references: 17728157
references: Ann Neurol. 2002 Sep;52(3):318-26
references: 12205644
references: Neurochem Int. 1996 May-Jun;28(5-6):497-500
references: 8792330
references: Epilepsy Res. 1994 Mar;17(3):221-31
references: 8013444
references: Neurobiol Dis. 2006 Sep;23(3):689-96
references: 16843675
references: Early Hum Dev. 1998 Nov;53(1):19-35
references: 10193924
references: Biochem Biophys Res Commun. 1972 Jan 31;46(2):849-54
references: 4400444
references: Free Radic Res. 2002 Nov;36(11):1139-46
references: 12592665
references: J Neurochem. 1995 Oct;65(4):1704-11
references: 7561868
references: Brain Res. 1999 Feb 13;818(2):228-35
references: 10082808
references: J Trauma. 2002 Jul;53(1):66-72
references: 12131392
references: J Appl Physiol (1985). 2000 Oct;89(4):1403-11
references: 11007575
references: Epilepsy Res. 2006 Aug;70(2-3):200-10
references: 16815680
references: Neurochem Res. 1995 Jan;20(1):31-8
references: 7739756
references: Eur J Pharmacol. 1993 Jan 12;230(2):151-8
references: 8422897
references: Intensive Care Med. 2004 Apr;30(4):556-75
references: 14767591
references: J Neurotrauma. 1995 Apr;12(2):159-67
references: 7629862
references: Brain Res Bull. 1996;41(3):131-41
references: 8886382
references: Brain Res. 1996 Apr 1;714(1-2):145-55
references: 8861619
references: J Korean Med Sci. 2001 Jun;16(3):335-41
references: 11410696
references: Arq Neuropsiquiatr. 2003 Jun;61(2B):430-3
references: 12894279
references: Free Radic Res. 2008 Aug;42(8):689-706
references: 18671159
references: Brain Res. 1990 Apr 30;514(2):300-4
references: 2162711
references: Cell Mol Neurobiol. 2010 Apr;30(3):381-7
references: 19798568
references: Epilepsia. 2002 Jun;43(6):574-80
references: 12060015
references: Arch Neurol. 1975 Aug;32(8):549-54
references: 125576
references: Exp Neurol. 1989 Nov;106(2):172-80
references: 2553470
references: Neurosci Lett. 2009 May 15;455(2):140-4
references: 19368863
references: Neuroreport. 1993 Apr;4(4):444-6
references: 7684618
references: Steroids. 1994 Jun;59(6):383-8
references: 7940617
references: Neurochem Int. 2006 Jan;48(2):75-82
references: 16290321
references: Epilepsy Res. 1992 Jul;12(2):141-9
references: 1327744
references: J Neurosurg. 1963 Jan;20:8-20
references: 14184991
references: Acta Neuropathol. 1994;87(3):250-8
references: 8009957
references: Eur J Neurosci. 1995 Aug 1;7(8):1674-83
references: 7582122
references: Biochem Pharmacol. 1961 Jul;7:88-95
references: 13726518
references: Brain Res. 1995 May 8;679(1):184-7
references: 7544223
references: Clin J Pain. 2003 Jul-Aug;19(4):233-9
references: 12840617
references: Epilepsia. 2000 Oct;41(10):1241-8
references: 11051118
references: Biochem Pharmacol. 1994 May 18;47(10):1725-30
references: 8204089
references: J Neurochem. 1974 Sep;23(3):611-3
references: 4279007
references: Can J Biochem. 1971 Nov;49(11):1217-24
references: 4257414
references: J Lab Clin Med. 1963 May;61:882-8
references: 13967893
references: Resuscitation. 1995 Jun;29(3):249-63
references: 7667556
references: Mol Psychiatry. 1997 Jul;2(4):300-10
references: 9246670
references: Seizure. 2010 Apr;19(3):165-72
references: 20149694
references: Neurosci Lett. 2007 Apr 18;416(3):227-30
references: 17383094
references: Acta Neuropathol. 1992;84(5):495-500
references: 1462764
references: Neurochem Res. 2001 Apr;26(4):369-74
references: 11495347
references: Life Sci. 1993;52(12):1031-8
references: 8445993
references: Curr Opin Pediatr. 1998 Jun;10(3):271-7
references: 9716889
references: J Neurochem. 1987 Sep;49(3):944-51
references: 3612132
references: Science. 1985 Sep 27;229(4720):1397-400
references: 4035356
references: J Neurotrauma. 2003 May;20(5):511-20
references: 12803982
references: Brain Res. 2002 Dec 13;957(2):330-7
references: 12445975
references: Adv Enzymol Relat Areas Mol Biol. 1987;59:103-67
references: 2880477
references: Brain Res. 2004 Aug 13;1017(1-2):85-91
references: 15261103
references: Eur J Neurosci. 2000 Apr;12(4):1431-9
references: 10762371
references: J Neurotrauma. 1993 Fall;10(3):263-71; discussion 273
references: 8258839
references: Neurosci Lett. 2000 Sep 22;291(3):179-82
references: 10984636
references: J Cereb Blood Flow Metab. 1993 Sep;13(5):796-802
references: 8360286
references: J Biol Chem. 1974 Nov 25;249(22):7130-9
references: 4436300
references: Behav Brain Res. 1983 Sep;9(3):315-35
references: 6639740
references: Anesthesiology. 1998 Feb;88(2):403-9
references: 9477061
references: Biochem Biophys Res Commun. 2007 Jan 19;352(3):662-7
references: 17157265
references: Comp Biochem Physiol B Biochem Mol Biol. 1996 Aug;114(4):327-35
references: 8840509
references: Brain Res. 2001 Mar 2;893(1-2):36-45
references: 11222990
references: Br J Anaesth. 2002 Feb;88(2):188-92
references: 11878653
references: J Neurosurg. 1993 Sep;79(3):354-62
references: 8360731
references: Neurotherapeutics. 2012 Jan;9(1):199-209
references: 21913006
references: J Cereb Blood Flow Metab. 1991 Jan;11(1):114-21
references: 1983995
references: Neurocrit Care. 2008;9(2):189-97
references: 18415032
references: Brain Res. 1988 Dec 20;475(2):305-16
references: 3214738
references: Ann Neurol. 2001 Jun;49(6):721-6
references: 11409423