title: Serum M65 as a biomarker for metastatic renal cell carcinoma.
abstract: BACKGROUND
  Effective cancer biomarkers for early detection, prognosis, or therapy response prediction are urgently need in metastatic RCC. M30 and M65 are released during apoptotic cell death and precisely reflect epithelial tumor cell death. The aim of this study was to determine the prognostic value of plasma M30 and M65 levels in predicting survival rates for patients with metastatic RCC.
  
  
  METHODS
  Thirty-nine patients with metastatic RCC and 39 healthy control subjects were included in this study. Serum M30 and M65 levels were measured by ELISA.
  
  
  RESULTS
  The median ages of the patients and control subjects were 60 and 58 years, respectively. No difference was detected in the median serum M30 level between the patients and control subjects (53.7 vs. 49.1 U/L; P = .31). The median serum M65 level was significantly higher in patients than in control subjects (334.0 vs. 179.1 U/L; P < .001). Receiver operating characteristic analysis revealed that the best cutoff value for serum M65 level for predicting progression-free survival (PFS) was 313.6 U/L. The median PFS of patients whose M65 levels were ≤ 313.6 U/L was better than that of patients whose M65 levels were > 313.6 U/L (P = .03).
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first study to evaluate serum M30 and M65 levels in patients with RCC. Serum M65 levels were significantly elevated in patients with metastatic RCC compared with healthy individuals. In addition, the serum M65 level could be predictive of PFS in patients with RCC.
authors: Department of Medical Oncology, Institute of Oncology, Istanbul University, Istanbul, Turkey. dr_ibrahim2000@yahoo.com
authors: Ibrahim
authors: I
authors: Yildiz
authors: Fatma
authors: F
authors: Sen
authors: Leyla
authors: L
authors: Kilic
authors: Serkan
authors: S
authors: Keskin
authors: Derya
authors: D
authors: Duranyildiz
authors: Elif
authors: E
authors: Bilgin
authors: Rian
authors: R
authors: Disci
authors: Meltem
authors: M
authors: Ekenel
authors: Emin
authors: E
authors: Darendeliler
authors: Sevil
authors: S
authors: Bavbek
authors: Mert
authors: M
authors: Basaran
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D053538:Keratin-18 / Q000097:blood*; D007680:Kidney Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood*; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D053538:Keratin-18; C561463:M30 cytokeratin-18 peptide, human; C577325:M65 antigen, human; D010446:Peptide Fragments
keywords: Apoptosis; Cytokeratin 18; Kidney cancer; Prognostic factors; Serum M65 level