title: Catabolism of 4-alkylphenols by Acinetobacter sp. OP5: genetic organization of the oph gene cluster and characterization of alkylcatechol 2, 3-dioxygenase.
abstract: In this study, a specific PCR primer set was successfully designed for alkylcatechol 2, 3-dioxygenase genes and applied to detect the presence of this biomarker in 4-t-octylphenol-degrading Acinetobacter sp. strain OP5. A gene cluster (ophRBA1A2A3A4A5A6CEH) encoding multicomponent phenol hydroxylase and alkylcatechol 2, 3-dioxygenase was then cloned from this strain and showed the highest homology to those involved in the published medium-chain alkylphenol gene clusters. The pure enzyme of recombinant cell harboring ophB showed meta-cleavage activities for 4-methylcatechol (1,435%), 4-ethylcatechol (982%), catechol (100%), 4-t-butylcatechol (16.6%), and 4-t-octylcatechol (3.2%). The results suggest that the developed molecular technique is useful and easy in detection of medium/long-chain alkylphenol degradation gene cluster. In addition, it also provides a better understanding of the distribution of biodegradative genes and pathway for estrogenic-active long-chain alkylphenols in bacteria.
authors: Institute of Systems Biology & Bioinformatics, National Central University, No. 300 Chung-da Rd., Chung-li 32001, Taiwan, ROC.
authors: Nguyen Ngoc
authors: NN
authors: Tuan
authors: Yi-Wen
authors: YW
authors: Lin
authors: Shir-Ly
authors: SL
authors: Huang
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008660:Metabolism; D005810:Multigene Family / Q000235:genetics*; D010636:Phenols / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D010636:Phenols; D050557:Catechol 2,3-Dioxygenase