title: Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins.
abstract: Ancylostoma ceylanicum belongs to a group of soil-transmitted helminths, which infect almost 576 mln people worldwide and are a major cause of anaemia and malnutrition. Upon contact with a permissive host, third-stage larvae (L3) residing in the environment become activated larvae (ssL3), a process associated with changes in the profile of gene expression. Ancylostoma secreted proteins (ASPs) are the major proteins secreted during larvae activation and play a crucial role in hookworm adaptation to parasitism. Here we report the cloning using RACE-PCR technique of three novel ASPs from the hookworm A. ceylanicum (Ace-asp-3, Ace-asp-4, and Ace-asp-5) and computational analysis of the protein sequences. All three proteins contain SCP (Sperm Coating Protein) domain characteristic for previously described ASP proteins. Real-time PCR analysis shows significant up-regulation of Ace-asp-3 and Ace-asp-5 expression in adult worms and correlated down-regulation in ssL3 larvae. On the other hand, expression of Ace-asp-4 was increased in ssL3 stages and decreased in adult parasites.
authors: Division of Parasitology and Parasitic Diseases, Department Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland.
authors: Anna M
authors: AM
authors: Siwińska
authors: Piotr
authors: P
authors: Bąska
authors: Emilia
authors: E
authors: Daniłowicz-Luebert
authors: Kamil
authors: K
authors: Januszkiewicz
authors: Ewa
authors: E
authors: Długosz
authors: Halina
authors: H
authors: Wędrychowicz
authors: Michael
authors: M
authors: Cappello
authors: Marcin
authors: M
authors: Wiśniewski
mesh_terms: D000722:Ancylostoma / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins