title: Template synthesis of test tube nanoparticles using non-destructive replication.
abstract: Nano test tubes are a promising delivery vehicle for a range of therapeutics, including small molecule drugs and biologics. However, current template synthesis methods of producing nano test tubes are prohibitively expensive and time consuming. Here, non-destructive template replication was used to increase nano test tube yield from porous alumina by more than a hundredfold. We demonstrate how to produce nano test tubes of several sizes and compositions, including hybrid tubes with different inner and outer surfaces for targeted surface chemistry. Nano test tubes were readily suspended and stored in aqueous solutions without the need for chemical treatment. These nano test tubes should find application as delivery vehicles for therapeutics, particularly for processive 'bionanoreactors' loaded with enzymes.
authors: Department of Biochemistry and Center for Structural Biology, University of Kentucky, Lexington, KY 40536, USA.
authors: Jonathan
authors: J
authors: Wagner
authors: Jingyuan
authors: J
authors: Yao
authors: David
authors: D
authors: Rodgers
authors: Bruce
authors: B
authors: Hinds
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D002138:Calibration; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D016062:Porosity; D014025:Titanium / Q000737:chemistry
chemical_list: D006046:Gold; D014025:Titanium; D000537:Aluminum Oxide
references: Nanomedicine (Lond). 2006 Jun;1(1):39-50
references: 17716208
references: Acc Chem Res. 2008 Dec;41(12):1721-30
references: 18712884
references: Mol Genet Metab. 2010 Jan;99(1):18-25
references: 19773189
references: Pharmacol Rev. 2001 Jun;53(2):283-318
references: 11356986
references: Biotechnol Bioeng. 2001 Dec 5;75(5):615-8
references: 11745138
references: Pharm Res. 2006 Jul;23(7):1417-50
references: 16779701
references: J Biomed Mater Res. 1998 May;40(2):324-35
references: 9549628
references: J Control Release. 2009 Aug 4;137(3):246-54
references: 19371766
references: Langmuir. 2011 Mar 15;27(6):2132-7
references: 21247183
references: Nat Mater. 2009 Jul;8(7):543-57
references: 19525947
references: Science. 1995 Jun 9;268(5216):1466-8
references: 17843666
references: Trends Biotechnol. 2008 Nov;26(11):639-46
references: 18804884
references: Chemistry. 2011 May 27;17(23):6296-302
references: 21563218
references: Nano Lett. 2008 Jul;8(7):1954-9
references: 18507449
references: Nature. 1994 May 26;369(6478):298-301
references: 8183367
references: Nanomedicine (Lond). 2010 Oct;5(8):1151-60
references: 21039193
references: Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11613-8
references: 18697944
references: Biomol Eng. 2001 Oct 31;18(4):143-77
references: 11576871
references: Nanomedicine (Lond). 2008 Jun;3(3):283-92
references: 18510424
references: FASEB J. 2005 Mar;19(3):311-30
references: 15746175
references: J Am Chem Soc. 2005 May 25;127(20):7316-7
references: 15898772