title: A testosterone specific competitive enzyme immunoassay for monitoring water quality.
abstract: Testosterone, an androgen and a primary male sex hormone, migrates through the environment in ways which could pose a threat to water quality and, subsequently, environmental and human health. This paper describes the development of a direct competitive enzyme-linked immunosorbent assay (ELISA) that is capable of measuring testosterone with high specificity. The testosterone ELISA displayed the IC20 (as the limit of detection) and IC50 values of 0.05 ± 0.01 μg L(-1) and 0.33 ± 0.18 μg L(-1), respectively. In addition, the assay showed <0.1 % cross-reactivity against structurally related steroidal compounds. However, this assay was found to be sensitive to environmental matrices such as certain metal ions, pH, and high humic acids, and sample clean-up to remove such interference was necessary before analysis. The analyses of 50 surface water samples collected in rural and urban areas in New South Wales, Australia showed that ELISA results correlated well with the androgenic activity measured by the recombinant yeast-based androgen screen assay.
authors: School of Chemical Engineering, Food Science and Technology, University of New South Wales, Sydney, NSW 2052, Australia.
authors: Chatchaporn
authors: C
authors: Uraipong
authors: Victor
authors: V
authors: Wong
authors: Nanju Alice
authors: NA
authors: Lee
mesh_terms: D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D009517:New South Wales; D013739:Testosterone / Q000032:analysis* / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000276:immunology
chemical_list: D014874:Water Pollutants, Chemical; D013739:Testosterone