title: Novel higher-order epigenetic regulation of the Bdnf gene upon seizures.
abstract: Studies in cultured cells have demonstrated the existence of higher-order epigenetic mechanisms, determining the relationship between expression of the gene and its position within the cell nucleus. It is unknown, whether such mechanisms operate in postmitotic, highly differentiated cell types, such as neurons in vivo. Accordingly, we examined whether the intranuclear positions of Bdnf and Trkb genes, encoding the major neurotrophin and its receptor respectively, change as a result of neuronal activity, and what functional consequences such movements may have. In a rat model of massive neuronal activation upon kainate-induced seizures we found that elevated neuronal expression of Bdnf is associated with its detachment from the nuclear lamina, and translocation toward the nucleus center. In contrast, the position of stably expressed Trkb remains unchanged after seizures. Our study demonstrates that activation-dependent architectural remodeling of the neuronal cell nucleus in vivo contributes to activity-dependent changes in gene expression in the brain.
authors: Nencki Institute of Experimental Biology PAS, 02-093, Warsaw, Poland.
authors: Agnieszka
authors: A
authors: Walczak
authors: Andrzej A
authors: AA
authors: Szczepankiewicz
authors: Blazej
authors: B
authors: Ruszczycki
authors: Adriana
authors: A
authors: Magalska
authors: Katarzyna
authors: K
authors: Zamlynska
authors: Joanna
authors: J
authors: Dzwonek
authors: Ewa
authors: E
authors: Wilczek
authors: Katarzyna
authors: K
authors: Zybura-Broda
authors: Marcin
authors: M
authors: Rylski
authors: Monika
authors: M
authors: Malinowska
authors: Michal
authors: M
authors: Dabrowski
authors: Teresa
authors: T
authors: Szczepinska
authors: Krzysztof
authors: K
authors: Pawlowski
authors: Marta
authors: M
authors: Pyskaty
authors: Jakub
authors: J
authors: Wlodarczyk
authors: Izabela
authors: I
authors: Szczerbal
authors: Marek
authors: M
authors: Switonski
authors: Marion
authors: M
authors: Cremer
authors: Grzegorz M
authors: GM
authors: Wilczynski
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000502:physiology; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000502:physiology*; D012640:Seizures / Q000235:genetics / Q000378:metabolism*; D014178:Translocation, Genetic / Q000502:physiology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020813:Receptor, trkB
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