title: First detection of Alaria alata mesocercariae in wild boars (Sus scrofa Linnaeus, 1758) from Bulgaria.
abstract: The trematode Alaria alata, an intestinal parasite of different carnivore species is widely distributed throughout Europe. The mesocercarial stages of Alaria spp. may infect almost all vertebrate species, including humans, and, in particular, omnivorous scavengers such as wild boars serve as paratenic hosts for the parasite. The introduction of the A. alata mesocercariae migration technique (AMT) opened the way to a reliable detection of Alaria spp. mesocercariae in different body tissues of their paratenic hosts. For the first time, it was possible to detect vital A. alata mesocercariae from two Bulgarian wild boars by means of this new method. In addition, polymerase chain reaction (PCR) examination of the respective parasitic DNA allowed the unequivocal species identification of the parasites as A. alata. Isolation and molecular biological identification of the parasite's developmental stages make significant contributions to completion of data on both the distribution of Alaria spp. in stocks of European game and the relationship between different Eurasian Alaria spp. isolates.
authors: Leipzig University, Faculty of Veterinary Medicine, Institute of Food Hygiene, An den Tierkliniken 1, D-04103 Leipzig, Germany.
authors: K
authors: K
authors: Riehn
authors: National Diagnostic and Research Veterinary Institute, National Reference Laboratory for Parasites 15A Pencho Slaveikov Blvd, 1606 Sofia, Bulgaria.
authors: N
authors: N
authors: Lalkovski
authors: Leipzig University, Faculty of Veterinary Medicine, Institute of Food Hygiene, An den Tierkliniken 1, D-04103 Leipzig, Germany.
authors: A
authors: A
authors: Hamedy
authors: Leipzig University, Faculty of Veterinary Medicine, Institute of Food Hygiene, An den Tierkliniken 1, D-04103 Leipzig, Germany.
authors: Ernst
authors: E
authors: Lücker
mesh_terms: D000818:Animals; D002031:Bulgaria; D018509:DNA, Helminth / Q000235:genetics; D007814:Larva; D008853:Microscopy; D016133:Polymerase Chain Reaction; D034421:Sus scrofa / Q000469:parasitology*; D014200:Trematoda / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth