title: Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion.
abstract: Hepatic triacylglycerol levels are governed through synthesis, degradation and export of this lipid. Here we demonstrate that enforced expression of hepatic lipase in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a significant decrease in the incorporation of fatty acids into cellular triacylglycerol and cholesteryl ester accompanied by attenuation of secretion of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion of intracellular lipid storage increased the expression of peroxisome proliferator-activated receptor α and its target genes and augmented oxidation of fatty acids. These data show that 1) hepatic lipase is active in the endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular lipid metabolism and lipoprotein secretion.
authors: Mazankowski Alberta Heart Institute, University of Alberta, Edmonton, Alberta, Canada.
authors: Bruce
authors: B
authors: Erickson
authors: Senthamil Paramadayalan
authors: SP
authors: Selvan
authors: Kerry W S
authors: KW
authors: Ko
authors: Karen
authors: K
authors: Kelly
authors: Ariel D
authors: AD
authors: Quiroga
authors: Lena
authors: L
authors: Li
authors: Randy
authors: R
authors: Nelson
authors: Kirst
authors: K
authors: King-Jones
authors: René L
authors: RL
authors: Jacobs
authors: Richard
authors: R
authors: Lehner
mesh_terms: D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism; D002478:Cells, Cultured; D002788:Cholesterol Esters / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D005227:Fatty Acids / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology*; D008049:Lipase / Q000378:metabolism*; D050356:Lipid Metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D001055:Apolipoproteins B; D002788:Cholesterol Esters; D005227:Fatty Acids; D008079:Lipoproteins, VLDL; D047493:PPAR alpha; D014280:Triglycerides; D008049:Lipase