title: A modified metabolic model for mixed culture fermentation with energy conserving electron bifurcation reaction and metabolite transport energy.
abstract: A modified metabolic model for mixed culture fermentation (MCF) is proposed with the consideration of an energy conserving electron bifurcation reaction and the transport energy of metabolites. The production of H2 related to NADH/NAD(+) and Fdred/Fdox is proposed to be divided in three processes in view of energy conserving electron bifurcation reaction. This assumption could fine-tune the intracellular redox balance and regulate the distribution of metabolites. With respect to metabolite transport energy, the proton motive force is considered to be constant, while the transport rate coefficient is proposed to be proportional to the octanol-water partition coefficient. The modeling results for a glucose fermentation in a continuous stirred tank reactor show that the metabolite distribution is consistent with the literature: (1) acetate, butyrate, and ethanol are main products at acidic pH, while the production shifts to acetate and propionate at neutral and alkali pH; (2) the main products acetate, ethanol, and butyrate shift to ethanol at higher glucose concentration; (3) the changes for acetate and butyrate are following an increasing hydrogen partial pressure. The findings demonstrate that our modified model is more realistic than previous proposed model concepts. It also indicates that inclusion of an energy conserving electron bifurcation reaction and metabolite transport energy for MCF is sound in the viewpoint of biochemistry and physiology.
authors: School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors: Fang
authors: F
authors: Zhang
authors: Yan
authors: Y
authors: Zhang
authors: Man
authors: M
authors: Chen
authors: Mark C M
authors: MC
authors: van Loosdrecht
authors: Raymond J
authors: RJ
authors: Zeng
mesh_terms: D000085:Acetates / Q000378:metabolism; D001692:Biological Transport; D002087:Butyrates / Q000378:metabolism; D004734:Energy Metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D018892:Proton-Motive Force
chemical_list: D000085:Acetates; D002087:Butyrates; D009243:NAD; D000431:Ethanol; D006859:Hydrogen; D005947:Glucose