title: Development of a surface plasmon resonance and nanomechanical biosensing hybrid platform for multiparametric reading.
abstract: We have developed a hybrid platform that combines two well-known biosensing technologies based on quite different transducer principles: surface plasmon resonance and nanomechanical sensing. The new system allows the simultaneous and real-time detection of two independent parameters, refractive index change (Δn), and surface stress change (Δσ) when a biomolecular interaction takes place. Both parameters have a direct relation with the mass coverage of the sensor surface. The core of the platform is a common fluid cell, where the solution arrives to both sensor areas at the same time and under the same conditions (temperature, velocity, diffusion, etc.).The main objective of this integration is to achieve a better understanding of the physical behaviour of the transducers during sensing, increasing the information obtained in real time in one single experiment. The potential of the hybrid platform is demonstrated by the detection of DNA hybridization.
authors: Nanobiosensors and Bioanalytical Applications Group, Research Center on Nanoscience and Nanotechnology CIN2(CSIC) and CIBER-BBN, 08193 Bellaterra, Barcelona, Spain. mar.alvarez@cin2.es
authors: Mar
authors: M
authors: Alvarez
authors: David
authors: D
authors: Fariña
authors: Alfonso M
authors: AM
authors: Escuela
authors: Jose Ramón
authors: JR
authors: Sendra
authors: Laura M
authors: LM
authors: Lechuga
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry; D055595:Mechanical Phenomena; D046210:Microfluidic Analytical Techniques; D036103:Nanotechnology / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization; D055096:Optical Devices; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014159:Transducers
chemical_list: D004277:DNA, Single-Stranded