title: Effect of normabaric hyperoxia treatment on neuronal damage following fluid percussion injury in the striatum of mice: a morphological approach.
abstract: Traumatic brain injury (TBI) causes significant mortality in most developing countries worldwide. At present, it is imperative to identify a treatment to address the devastating post-TBI consequences. Therefore, the present study has been performed to assess the specific effect of immediate exposure to normabaric hyperoxia (NBO) after fluid percussion injury (FPI) in the striatum of mice. To execute FPI, mice were anesthetised and sorted into (i) a TBI group, (ii) a sham group without injury and (iii) a TBI group treated with immediate exposure to NBO for 3 h. Afterwards, brains were harvested for morphological assessment. The results revealed no changes in morphological and neuronal damage in the sham group as compared to the TBI group. Conversely, the TBI group showed severe morphological changes as well as neuronal damage as compared to the TBI group exposed to NBO for 3 h. Interestingly, our findings also suggested that NBO treatment could diminish the neuronal damage in the striatum of mice after FPI. Neuronal damage was evaluated at different points of injury and the neighbouring areas using morphology, neuronal apoptotic cell death and pan-neuronal markers to determine the complete neuronal structure. In conclusion, immediate exposure to NBO following FPI could be a potential therapeutic approach to reduce neuronal damage in the TBI model.
authors: Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia.
authors: Sangu
authors: S
authors: Muthuraju
authors: Soumya
authors: S
authors: Pati
authors: Mohammad
authors: M
authors: Rafiqul
authors: Jafri Malin
authors: JM
authors: Abdullah
authors: Hasnan
authors: H
authors: Jaafar
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001469:Barotrauma; D015415:Biomarkers / Q000378:metabolism; D001930:Brain Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D003342:Corpus Striatum / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006651:Histocytochemistry; D018496:Hyperoxia / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010100:Oxygen / Q000494:pharmacology*; D013238:Stereotaxic Techniques
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D010100:Oxygen
references: J Neurotrauma. 1994 Jun;11(3):289-301
references: 7996583
references: Semin Clin Neuropsychiatry. 1998 Jul;3(3):160-175
references: 10085204
references: Br J Anaesth. 2003 Jun;90(6):774-86
references: 12765894
references: J Neurosci. 2005 Apr 13;25(15):3763-73
references: 15829628
references: J Neurosurg. 2004 Sep;101(3):435-44
references: 15352601
references: J Neurochem. 2000 Feb;74(2):740-53
references: 10646526
references: J Neurotrauma. 2010 Aug;27(8):1429-38
references: 20528171
references: J Neurochem. 2000 Nov;75(5):2178-89
references: 11032908
references: Neuroscience. 2002;110(4):605-16
references: 11934469
references: Stroke. 2002 Mar;33(3):809-15
references: 11872908
references: J Neurosci. 1998 Aug 1;18(15):5663-72
references: 9671657
references: J Neurosurg. 1991 Mar;74(3):407-14
references: 1899694
references: Acta Neuropathol. 2000 Feb;99(2):117-24
references: 10672317
references: Neurosurgery. 1996 Jun;38(6):1176-95
references: 8727150
references: J Neurosurg. 1989 May;70(5):774-9
references: 2709117
references: Blood. 2010 Apr 1;115(13):2709-18
references: 20124512
references: Ann Neurol. 1994 Oct;36(4):557-65
references: 7944288
references: J Neurotrauma. 2000 Oct;17(10):811-29
references: 11063050
references: J Cereb Blood Flow Metab. 1996 May;16(3):481-9
references: 8621753
references: Cent Nerv Syst Trauma. 1987;4(2):119-34
references: 3690695
references: Eur J Anaesthesiol. 1996 May;13(3):247-68
references: 8737117
references: J Neurosurg. 1999 Oct;91(4):653-9
references: 10507388
references: J Cereb Blood Flow Metab. 2001 Aug;21(8):914-20
references: 11487726
references: J Neurotrauma. 2010 May;27(5):889-99
references: 20233041
references: J Neurotrauma. 1998 Mar;15(3):217-29
references: 9528921
references: J Neurotrauma. 2006 Sep;23(9):1330-42
references: 16958585
references: J Neurosurg. 2001 Mar;94(3):403-11
references: 11235943
references: Acta Neurochir (Wien). 1996;138(1):90-8
references: 8686532
references: J Neurosurg. 1997 Mar;86(3):511-8
references: 9046309
references: Chin J Traumatol. 2002 Feb;5(1):24-7
references: 11835752
references: Acta Neuropathol. 1991;83(1):66-71
references: 1792866
references: Acta Neurochir Suppl. 1996;67:40-4
references: 8870800
references: Neuroscientist. 2002 Aug;8(4):323-34
references: 12194501
references: Acta Neuropathol. 1988;76(4):347-58
references: 2459896
references: Neuroscience. 2007 Jul 29;147(4):906-18
references: 17600628