title: US elastography-derived shear wave velocity helps distinguish acutely inflamed from fibrotic bowel in a Crohn disease animal model.
abstract: OBJECTIVE
  To determine if acoustic radiation force impulse elastography-derived bowel wall shear wave velocity (SWV) allows distinction of acutely inflamed from fibrotic intestine in a Crohn disease animal model.
  
  
  METHODS
  University Committee on the Use and Care of Animals approval was obtained. An acute inflammation Crohn disease model was produced by treating eight Lewis rats with a single administration of trinitrobenzenesulfonic acid (TNBS) enema, with imaging performed 2 days later in the surviving six rats. Colonic fibrosis in an additional eight Lewis rats was achieved by administering repeated TNBS enemas during 4 weeks, with imaging performed in the surviving seven rats 7 days later to allow acute inflammation resolution. Nine transcutaneous bowel wall SWV measurements were obtained from the colon in all rats without and with applied strain. Mean SWVs without and with applied strain were compared between animal cohorts by using the Student t test, and receiver operating characteristic (ROC) curves were created to assess diagnostic performance.
  
  
  RESULTS
  Mean bowel wall SWVs were significantly higher for fibrotic versus acute inflammation cohort of rats at 0% (3.4 ± 1.1 vs 2.3 ± 0.5 m/sec; P = .047) and 30% (6.3 ± 2.2 vs 3.6 ± 0.9 m/sec; P = .02) applied strain. Both acute inflammation and fibrotic cohort of rats demonstrated linear increases in mean SWV with increasing applied strain, with significantly different mean slopes (P = .02) and y-intercepts (P = .02). The area under the ROC curve of the SWV ratio (mean SWV/applied strain) for differentiating histopathologically confirmed fibrotic from inflamed bowel was 0.971.
  
  
  CONCLUSIONS
  Bowel wall SWV helps distinguish acutely inflamed from fibrotic intestine in a Crohn disease animal model.
authors: Department of Radiology, Section of Pediatric Radiology, University of Michigan Health System, C.S. Mott Children's Hospital, 1540 E Hospital Dr, Ann Arbor, MI 48109-4252, USA. jonadill@med.umich.edu
authors: Jonathan R
authors: JR
authors: Dillman
authors: Ryan W
authors: RW
authors: Stidham
authors: Peter D R
authors: PD
authors: Higgins
authors: David S
authors: DS
authors: Moons
authors: Laura A
authors: LA
authors: Johnson
authors: Jonathan M
authors: JM
authors: Rubin
mesh_terms: D000818:Animals; D003424:Crohn Disease / Q000000981:diagnostic imaging* / Q000473:pathology; D003937:Diagnosis, Differential; D004195:Disease Models, Animal; D054459:Elasticity Imaging Techniques / Q000379:methods*; D005260:Female; D005355:Fibrosis / Q000000981:diagnostic imaging / Q000473:pathology; D016014:Linear Models; D012372:ROC Curve; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew
references: Ultrasound Med Biol. 2012 Jan;38(1):50-61
references: 22104533
references: Interface Focus. 2011 Aug 6;1(4):553-64
references: 22419986
references: Ultrasound Med Biol. 2004 Jun;30(6):761-71
references: 15219956
references: Am J Gastroenterol. 2010 Feb;105(2):289-97
references: 19861953
references: Eur Radiol. 2006 Nov;16(11):2410-7
references: 16752151
references: Radiology. 2011 Apr;259(1):127-35
references: 21324841
references: Radiology. 2010 Jul;256(1):297-303
references: 20505064
references: Abdom Imaging. 2011 Apr;36(2):142-8
references: 20464392
references: Abdom Imaging. 2004 Nov-Dec;29(6):648-52
references: 15162232
references: Eur Rev Med Pharmacol Sci. 2006 Jan-Feb;10(1):23-6
references: 16494107
references: Clin Hemorheol Microcirc. 2008;40(2):143-55
references: 19029639
references: Abdom Imaging. 2008 Sep-Oct;33(5):589-97
references: 18172707
references: Inflamm Bowel Dis. 2012 May;18(5):849-56
references: 21710535
references: Radiology. 2012 Feb;262(2):680-8
references: 22157203
references: Phys Med Biol. 2012 Feb 7;57(3):R35-73
references: 22222839
references: Int Rev Immunol. 2000;19(1):51-62
references: 10723677
references: Ultrason Imaging. 1991 Apr;13(2):111-34
references: 1858217
references: Nat Protoc. 2007;2(3):541-6
references: 17406617
references: Gut. 2001 Dec;49(6):777-82
references: 11709511
references: Radiology. 2009 Aug;252(2):595-604
references: 19703889
references: Eur J Radiol. 2009 Mar;69(3):438-44
references: 19070446
references: Scand J Gastroenterol. 2005 Aug;40(8):985-91
references: 16173144
references: Br J Radiol. 2009 Feb;82(974):112-7
references: 18955414
references: Eur J Radiol. 2007 Apr;62(1):114-21
references: 17239555
references: Gastroenterol Hepatol (N Y). 2009 Oct;5(10):705-715
references: 37967416
references: Phys Med Biol. 2009 Mar 7;54(5):1191-207
references: 19182325
references: Eur Radiol. 2012 Oct;22(10):2130-7
references: 22610533
references: Inflamm Bowel Dis. 2002 Jul;8(4):244-50
references: 12131607
references: Am J Gastroenterol. 2006 May;101(5):1030-8
references: 16606351
references: Inflamm Bowel Dis. 2004 Jan;10(1):55-60
references: 15058528
references: Ultrasound Med Biol. 2008 Jun;34(6):902-12
references: 18294759
references: Gastroenterology. 2011 Sep;141(3):819-826.e1
references: 21784048
references: Radiology. 2010 Aug;256(2):640-7
references: 20529989
references: Inflamm Bowel Dis. 2012 Mar;18(3):460-71
references: 21761511
references: Gut. 2005 Feb;54(2):237-41
references: 15647188
references: Eur Radiol. 2010 Aug;20(8):1945-51
references: 20379822
references: Inflamm Bowel Dis. 2011 Apr;17(4):984-93
references: 20722053
references: Ultraschall Med. 2011 Apr;32(2):154-9
references: 20449794
references: Radiology. 2009 Oct;253(1):241-8
references: 19635834
references: Ultrasound Med Biol. 2010 Feb;36(2):202-8
references: 20018432
references: Digestion. 2011;83(4):263-8
references: 21273775
references: J Nucl Med. 2009 Nov;50(11):1751-9
references: 19837758