title: Characterization of a novel cyfluthrin-degrading bacterial strain Brevibacterium aureum and its biochemical degradation pathway.
abstract: Brevibacterium aureum DG-12, a new bacterial strain isolated from active sludge, was able to degrade and utilize cyfluthrin as a growth substrate in the mineral medium. Response surface methodology using central composite rotatable design of cultural conditions was successfully employed for optimization resulting in 88.6% degradation of cyfluthrin (50mgL(-1)) within 5days. The bacterium degraded cyfluthrin by cleavage of both the carboxylester linkage and diaryl bond to form 2,2,3,3-tetramethyl-cyclopropanemethanol, 4-fluoro-3-phenexy-benzoic acid, 3,5-dimethoxy phenol, and phenol, and subsequently transformed these compounds with a maximum specific degradation rate, half-saturation constant and inhibition constant of 1.0384day(-1), 20.4967mgL(-1), and 141.9013mgL(-1), respectively. A novel degradation pathway for cyfluthrin was proposed based on analysis of these metabolites. In addition, this strain was found capable of degrading a wide range of synthetic pyrethroid insecticides. Our results suggest that B. aureum DG-12 may be an ideal microorganism for bioremediation of the pyrethroid-contaminated environments.
authors: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China.
authors: Shaohua
authors: S
authors: Chen
authors: Yi Hu
authors: YH
authors: Dong
authors: Changqing
authors: C
authors: Chang
authors: Yinyue
authors: Y
authors: Deng
authors: Xi Fen
authors: XF
authors: Zhang
authors: Guohua
authors: G
authors: Zhong
authors: Haiwei
authors: H
authors: Song
authors: Meiying
authors: M
authors: Hu
authors: Lian-Hui
authors: LH
authors: Zhang
mesh_terms: D001673:Biodegradation, Environmental; D001951:Brevibacterium / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D009570:Nitriles / Q000378:metabolism*; D011722:Pyrethrins / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology
chemical_list: D004785:Environmental Pollutants; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012722:Sewage; C052570:cyfluthrin