title: Acinetobacter puyangensis sp. nov., isolated from the healthy and diseased part of Populus xeuramericana canker bark.
abstract: Two Gram-negative, non-motile, rod-shaped strains, BQ4-1(T) and NHI3-2, isolated respectively from the healthy and diseased part of Populus ×euramericana canker bark, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of the two strains in the genus Acinetobacter, with genomic DNA G+C contents (42.5-43 mol%) within the range observed for this genus (38-47 mol%) and 9-octadecenoic acid (C18 : 1ω9c, 39.87 %), hexadecanoic acid (C16 : 0, 11.26 %) and summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c, 18.90 %) as major fatty acids. Phylogenetic analysis based on 16S rRNA, rpoB and gyrB gene sequences revealed that strains BQ4-1(T) and NHI3 did not cluster with any species with validly published names, and formed a distinct cluster with 99-100 % bootstrap support on three phylogenetic trees within the genus Acinetobacter. Acid was not produced from d-glucose, and haemolysis was not observed on agar media supplemented with sheep erythrocytes. On the basis of phenotypic, genotypic and phylogenetic characteristics, the two strains are considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter puyangensis sp. nov. is proposed. The type strain is BQ4-1(T) (= CFCC 10780(T) = JCM 18011(T)).
authors: Beijing Forestry University, The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing 100083, China.|The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors: Yong
authors: Y
authors: Li
authors: The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors: Chun-Gen
authors: CG
authors: Piao
authors: Beijing Forestry University, The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing 100083, China.
authors: Yu-Chao
authors: YC
authors: Ma
authors: Beijing Forestry University, The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing 100083, China.
authors: Wei
authors: W
authors: He
authors: Heze Forest Protection Station, Heze 274002, China.
authors: Hai-Ming
authors: HM
authors: Wang
authors: Puyang Academy of Forestry, Puyang 457000, China.
authors: Ju-Pu
authors: JP
authors: Chang
authors: Puyang Academy of Forestry, Puyang 457000, China.
authors: Li-Min
authors: LM
authors: Guo
authors: The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors: Xi-Zhuo
authors: XZ
authors: Wang
authors: Puyang Academy of Forestry, Puyang 457000, China.
authors: Shou-Jiang
authors: SJ
authors: Xie
authors: The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors: Min-Wei
authors: MW
authors: Guo
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S