title: Visualization of the mycelia of wood-rotting fungi by fluorescence in situ hybridization using a peptide nucleic acid probe.
abstract: White rot fungus, Phanerochaete chrysosporium, and brown rot fungus, Postia placenta, grown on agar plates, were visualized by fluorescence in situ hybridization (FISH) using a peptide nucleic acid (PNA) probe. Mycelia grown on wood chips were also clearly detected by PNA-FISH following blocking treatment. To the best of our knowledge, this is the first report on the visualization of fungi in wood by FISH.
authors: Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
authors: Yuji
authors: Y
authors: Nakada
authors: Satoshi
authors: S
authors: Nakaba
authors: Hiroshi
authors: H
authors: Matsunaga
authors: Ryo
authors: R
authors: Funada
authors: Makoto
authors: M
authors: Yoshida
mesh_terms: D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence; D025282:Mycelium / Q000254:growth & development / Q000648:ultrastructure*; D015341:Nucleic Acid Probes / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D020075:Phanerochaete / Q000254:growth & development / Q000648:ultrastructure*; D020072:Polyporales / Q000254:growth & development / Q000648:ultrastructure*; D012337:RNA, Ribosomal, 18S / Q000032:analysis; D014934:Wood / Q000382:microbiology
chemical_list: D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; D012337:RNA, Ribosomal, 18S