title: Absence of CC chemokine receptors 2a and 2b from human adipose lineage cells.
abstract: Previous results have suggested the existence of receptors for monocyte chemoattractant protein-1 (MCP-1), CC chemokine receptors 2 (CCR2), in human adipocytes and their involvement in mediating effects of MCP-1 on adipocyte functions. However, the presence of CCR2 present on non-adipose-lineage cells of adipose tissue has not been excluded. We have used human Simpson-Golabi-Behmel-Syndrome (SGBS) preadipocytes and in-vitro-differentiated mature adipocytes to investigate the expression of CCR2 in human (pre)adipocytes. We found that the cells are devoid of CCR2 receptor protein and mRNA expression and fail to respond to treatment with all known CCR2 chemokine agonists. CCR2 is also absent from (pre)adipocytes prepared in vitro from human multipotent adipose-derived stem cells, bone-marrow-derived mesenchymal stem cells, or from primary (pre)adipocytes. Conditions mimicking proinflammatory changes in adipose tissue did not induce CCR2 receptor expression. We conclude that CCR2 is absent from human adipose lineage cells. Functional effects previously described for MCP-1 in human adipose tissue may be mediated indirectly through paracrine effects on non-adipose-lineage cells or by a (pre)adipocyte receptor for MCP-1 distinct from CCR2.
authors: Institute of Pharmacology and Toxicology, Ulm University Medical Center, Ulm, Germany.
authors: Carolin
authors: C
authors: Koenig
authors: Pamela
authors: P
authors: Fischer-Posovszky
authors: Markus T
authors: MT
authors: Rojewski
authors: Daniel
authors: D
authors: Tews
authors: Hubert
authors: H
authors: Schrezenmeier
authors: Martin
authors: M
authors: Wabitsch
authors: Peter
authors: P
authors: Gierschik
authors: Barbara
authors: B
authors: Moepps
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D006801:Humans; D018945:Monocyte Chemoattractant Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D054390:Receptors, CCR2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D018945:Monocyte Chemoattractant Proteins; D012333:RNA, Messenger; D054390:Receptors, CCR2