title: [Expressions of CXCL9, CXCL10 and CXCL11 in renal tubular epithelial cells induced by IFN-γ].
abstract: OBJECTIVE
  To investigate the effect of interferon-γ (IFN-γ) on the releases of CXCL9, CXCL10 and CXCL11 in renal proximal tubular epithelial cells (HK-2) and explore their functions.
  
  
  METHODS
  After stimulated by IFN-γ for different time, the HK-2 cells were analyzed by real-time PCR to detect the expressions of CXCL9, CXCL10 and CXCL11 mRNA, and were tested by ELISA to quantify the releases of CXCL9, CXCL10 and CXCL11 in supernatants. After activation of lymphocytes, cells were examined by flow cytometry to determine the CXCR3 expression, while chemotaxis assay was applied to evaluate the chemotactic effect of the supernatants of IFN-stimulated HK-2 cells on lymphocytes.
  
  
  RESULTS
  Compared with the control group, the expressions of CXCL9, CXCL10 and CXCL11 mRNA in the IFN-stimulated group were significantly increased 12 h after stimulation, and peaked at 48, 24 and 24 h, respectively. The levels of CXCL9, CXCL10 and CXCL11 proteins began to rise at 12 h and reached the peak at 72, 48 and 72 h, respectively. Compared with the control group, the expression of CXCR3 was markedly increased in the activated lymphocytes. The activated lymphocytes were recruited by the culture supernatants of IFN-stimulated HK-2 cells and the pretreatment of CXCR3 antibody inhibited this chemotactic effect.
  
  
  CONCLUSIONS
  IFN-γ can significantly up-regulate the expressions of chemokines such as CXCL9, CXCL10 and CXCL11 at both mRNA and protein levels in HK-2 cells.
authors: Department of Clinical Laboratory, Fujian University of Traditional Chinese Medicine, Fuzhou, China. fjlinqing@126.com
authors: Qing
authors: Q
authors: Lin
authors: Yanfang
authors: Y
authors: Song
authors: Xianjin
authors: X
authors: Zhu
authors: Shunliang
authors: S
authors: Yang
authors: Jian
authors: J
authors: Zheng
mesh_terms: D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D054371:Chemokine CXCL11 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D054370:Chemokine CXCL9 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C114753:CXCL10 protein, human; C515202:CXCL11 protein, human; C080810:CXCL9 protein, human; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D054370:Chemokine CXCL9; D012333:RNA, Messenger; D007371:Interferon-gamma