title: Prevention of neutrophil protein oxidation with vitamins C and E diet supplementation without affecting the adaptive response to exercise.
abstract: The authors studied the effects of antioxidant diet supplementation with an almond-based beverage on neutrophil antioxidants, nitrite, and protein oxidative alterations after exercise. Fourteen trained male amateur runners were randomly assigned in a double-blind fashion to receive antioxidant supplementation (152 mg/d vitamin C and 50 mg/d vitamin E) or placebo using an almond-based beverage for 1 mo and participated in a half-marathon race. Blood samples were taken before and after the half-marathon and after 3 hr recovery. Supplementation significantly increased basal neutrophil vitamin C compared with placebo (p < .05). Exercise increased neutrophil vitamin E levels in the supplemented group and decreased vitamin C in both groups after recovery (p < .05). Neutrophil catalase and glutathione peroxidase gene expression and nitrite levels were significantly increased as result of exercise (p < .05). Nitrotyrosine and protein carbonyl derivates increased only in the placebo group after exercise (p < .05), and these values remained high at recovery. No significant differences were evidenced in caspase-3 activity and DNA damage. Antioxidant supplementation with vitamins C and E reduced the exercise-induced oxidation of proteins in neutrophils, without altering the antioxidant adaptive response, as evidenced by the increased catalase and glutathione peroxidase gene expression.
authors: Laboratory of Physical Activity Sciences, IUNICS, University of Balearic Islands, Illes Balears, Spain.
authors: Antoni
authors: A
authors: Sureda
authors: Miguel D
authors: MD
authors: Ferrer
authors: Antonia
authors: A
authors: Mestre
authors: Josep A
authors: JA
authors: Tur
authors: Antoni
authors: A
authors: Pons
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D004249:DNA Damage; D019587:Dietary Supplements; D004311:Double-Blind Method; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D009573:Nitrites / Q000097:blood; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D012420:Running / Q000502:physiology*; D014810:Vitamin E / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D009573:Nitrites; D014815:Vitamins; D014810:Vitamin E; D005979:Glutathione Peroxidase; D053148:Caspase 3; D001205:Ascorbic Acid