title: Surface modification of lipid nanocapsules with polysaccharides: from physicochemical characteristics to in vivo aspects.
abstract: Attaching polysaccharides to the surface of nanoparticles offers the possibility of modifying the physicochemical and biological properties of the core particles. The surface of lipid nanocapsules (LNCs) was modified by post-insertion of amphiphilic lipochitosan (LC) or lipodextran (LD). Modelling of these LNCs by the drop tensiometer technique revealed that the positively charged LC made the LNC surface more rigid, whereas the neutral, higher M(W) LD had no effect on the surface elasticity. Both LNC-LC and LNC-LD activated the complement system more than the blank LNC, thus suggesting increased capture by the mononuclear phagocyte system. In vitro, the positively charged LNC-LC were more efficiently bound by the model HEK293(β3) cells compared to LNC and LNC-LD. Finally, it was observed that neither LC nor LD changed the in vivo biodistribution properties of LNCs in mice. These polysaccharide-coated LNCs, especially LNC-LC, are promising templates for targeting ligands (e.g. peptides, proteins) or therapeutic molecules (e.g. siRNA).
authors: LUNAM Université, Université d'Angers, INSERM U1066, IBS-CHU Angers, Angers, France. samuli.hirsjarvi@univ-angers.fr
authors: Samuli
authors: S
authors: Hirsjärvi
authors: Sandrine
authors: S
authors: Dufort
authors: Guillaume
authors: G
authors: Bastiat
authors: Patrick
authors: P
authors: Saulnier
authors: Catherine
authors: C
authors: Passirani
authors: Jean-Luc
authors: JL
authors: Coll
authors: Jean-Pierre
authors: JP
authors: Benoît
mesh_terms: D003167:Complement Activation; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008962:Models, Theoretical; D053769:Nanocapsules; D011134:Polysaccharides / Q000737:chemistry*; D013499:Surface Properties; D014018:Tissue Distribution
chemical_list: D008055:Lipids; D053769:Nanocapsules; D011134:Polysaccharides