title: Activities of free and encapsulated Lactobacillus acidophilus LA5 or Lactobacillus casei 01 in processed longan juices on exposure to simulated gastrointestinal tract.
abstract: BACKGROUND
  Fruit drinks containing probiotics are gaining interest in the global marketplace. For example, longan juice, containing carbohydrate and various bioactive components, is a potentially health-promoting beverage as well as probiotic carrier for human consumption. In this study, high-pressure and thermal processes were applied to eliminate competitive micro-organisms in longan juice prior to the addition of Lactobacillus acidophilus LA5 or Lactobacillus casei 01. The activities of these probiotics in a simulated gastrointestinal tract were also investigated.
  
  
  RESULTS
  Encapsulated probiotics could survive in the acidic environment of the stomach and small intestine, while the free cells were completely eliminated. In the colon experiment, the influence of encapsulated L. casei 01 on colon lactobacilli was significantly greater than that of encapsulated L. acidophilus LA5. Both encapsulated probiotics suspended in processed longan juices led to extensive increases in the formation of lactic acid and short-chain fatty acids (SCFA). Acetate was the major SCFA produced by colon bacteria, followed by propionate and butyrate. The discernible clear zone suggested that L. casei 01 provided greater antibacterial activity than L. acidophilus LA5.
  
  
  CONCLUSIONS
  Both encapsulated probiotics along with processed longan juice led to significant increases in colon lactobacilli, lactic acid and SCFA formation.
authors: Division of Food Science, Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand.
authors: Pittaya
authors: P
authors: Chaikham
authors: Arunee
authors: A
authors: Apichartsrangkoon
authors: Srivilai
authors: S
authors: Worametrachanon
authors: Wissanee
authors: W
authors: Supraditareporn
authors: Ekachai
authors: E
authors: Chokiatirote
authors: Tom
authors: T
authors: Van der Wiele
mesh_terms: D001628:Beverages / Q000032:analysis / Q000382:microbiology*; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D018914:Cells, Immobilized; D005285:Fermentation; D005519:Food Preservation; D005638:Fruit / Q000737:chemistry / Q000382:microbiology; D005750:Gastric Juice / Q000737:chemistry / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D007419:Intestinal Secretions / Q000737:chemistry / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000254:growth & development* / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000254:growth & development* / Q000378:metabolism; D056265:Microbial Interactions; D050296:Microbial Viability; D008863:Microspheres; D008954:Models, Biological; D061352:Pasteurization; D011312:Pressure; D019936:Probiotics; D013785:Thailand; D064028:Tracheophyta / Q000737:chemistry / Q000382:microbiology*