title: Combined fluorescence-Raman spectroscopic setup for the diagnosis of melanocytic lesions.
abstract: Two optical fibre-based probes for spectroscopic measurements on human tissues were designed and developed. The two probes combine fluorescence and Raman spectroscopy in a multimodal approach. The fluorescence excitation was provided by two laser diodes emitting in the UV (378 nm) and in the visible (445 nm) range, while a third source in the NIR (785 nm) was used for Raman. The device was tested on freshly excised human skin biopsies clinically diagnosed as malignant melanoma, melanocytic nevus, or healthy skin. Discrimination of lesions based on their fluorescence and Raman spectra showed good correlation with the subsequent histological examination.
authors: National Institute of Optics, National Research Council INO-CNR, Largo E. Fermi 6, 50125, Florence, Italy; European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy. rcicchi@lens.unifi.it.
authors: Riccardo
authors: R
authors: Cicchi
authors: Alessandro
authors: A
authors: Cosci
authors: Susanna
authors: S
authors: Rossari
authors: Dimitrios
authors: D
authors: Kapsokalyvas
authors: Enrico
authors: E
authors: Baria
authors: Vincenza
authors: V
authors: Maio
authors: Daniela
authors: D
authors: Massi
authors: Vincenzo
authors: V
authors: De Giorgi
authors: Nicola
authors: N
authors: Pimpinelli
authors: Francesco
authors: F
authors: Saverio Pavone
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000473:pathology; D008875:Middle Aged; D009508:Nevus, Pigmented / Q000175:diagnosis* / Q000473:pathology; D055100:Optical Fibers; D012867:Skin / Q000473:pathology; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D055815:Young Adult
keywords: Raman spectroscopy; dermatology; fluorescence spectroscopy