title: Directly from the source: endogenous preparations of molecular machines.
abstract: Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.
authors: Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre, Macromolecular Crystallography Group, c/Melchor Fdez. Almagro 3, 28029 Madrid, Spain.
authors: Pablo
authors: P
authors: Mesa
authors: Aurélien
authors: A
authors: Deniaud
authors: Guillermo
authors: G
authors: Montoya
authors: Christiane
authors: C
authors: Schaffitzel
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000378:metabolism; D003460:Crystallization; D004274:DNA, Recombinant / Q000235:genetics; D030562:Databases, Protein; D006801:Humans; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000302:isolation & purification*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D015003:Yeasts / Q000737:chemistry / Q000378:metabolism
chemical_list: D004274:DNA, Recombinant; D046912:Multiprotein Complexes; D011994:Recombinant Proteins