title: Water-based slow injection ultrasound-assisted emulsification microextraction for the determination of deoxynivalenol and de-epoxy-deoxynivalenol in maize and pork samples.
abstract: A novel water-based slow injection ultrasound-assisted emulsification microextraction (SI-USAEME) method followed by ultra-high performance liquid chromatography-tandem mass spectrometry analysis was developed for the rapid pretreatment and determination of deoxynivalenol (DON) and its metabolite, de-epoxy-deoxynivalenol (DOM-1), in maize and pork samples. After optimization, the method recoveries for DON and DOM-1 ranged from 73 to 85 % with intraday and interday variations less than 9.4 and 9.2 %, respectively. The limits of detection for DON were 4.2 μg kg(-1) in maize and 6.2 and 5.9 μg kg(-1) for DON and DOM-1, respectively, in pork. In addition, an immunoaffinity column (IAC) was prepared. A study comparing the IAC cleanup method, the solid-phase extraction (SPE) cleanup method, and the proposed SI-USAEME method was presented. The water-based SI-USAEME method could become a simple, low-cost alternative to the conventional IAC and SPE method. The method was successfully applied to the analysis of commercial maize and pork products.
authors: Department of Applied Chemistry, China Agricultural University, Beijing, China.
authors: Songqing
authors: S
authors: Li
authors: Yanshen
authors: Y
authors: Li
authors: Ying
authors: Y
authors: Wang
authors: Wenfeng
authors: W
authors: Zhou
authors: Haixiang
authors: H
authors: Gao
authors: Suxia
authors: S
authors: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057230:Limit of Detection; D008460:Meat / Q000032:analysis* / Q000382:microbiology; D009183:Mycotoxins / Q000032:analysis / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D014255:Trichothecenes / Q000032:analysis / Q000302:isolation & purification*; D014465:Ultrasonics; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; C048238:deepoxy-deoxynivalenol; C007262:deoxynivalenol