title: Hcrcn81 is upregulated by rapamycin treatment in human colorectal adenocarcinoma cells.
abstract: The aim of the present study was to determine the role of hcrcn81 in the regulation of the mammalian target of rapamycin (mTOR) pathway in human colorectal adenocarcinoma cells. The effect of rapamycin treatment on hcrcn81 expression was evaluated by examining the mRNA and protein expression of hcrcn81 in rapamycin‑treated human colon carcinoma cell lines, SW480 and LoVo, using real‑time PCR and western blot analysis, respectively. The results demonstrated that mRNA and protein levels of hcrcn81 were elevated following rapamycin treatment in these cell lines, indicating that hcrcn81 expression is upregulated by rapamycin treatment in human colorectal adenocarcinoma cells. Observations of the current study indicate that hcrcn81 may play a role in tumorigenesis by regulating the mTOR signaling pathway.
authors: Department of Anatomy, Basic Medical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors: Xiaoxia
authors: X
authors: Wen
authors: Lei
authors: L
authors: Dong
authors: Jianjun
authors: J
authors: Zhu
authors: Yao
authors: Y
authors: Chen
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000235:genetics
chemical_list: C586327:HCRCN81 protein, human; D009363:Neoplasm Proteins; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus