title: Measurement of barley β-glucan concentration in the plasma by sandwich ELISA using rat dectin-1.
abstract: We used recombinant rat dectin-1 proteins to newly establish sandwich ELISA for determining barley β-glucan (BβG). The ELISA method had a working range of 15-4,000 µg/L. Plasma BβG was detectable up to 24 h after an intravenous administration of BβG (1 mg/kg of body weight) to rats. This method may be an effective tool for investigating the immune modulatory effects of BβG.
authors: Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Ohya, Shizuoka, Japan.
authors: Shingo
authors: S
authors: Hino
authors: Hikaru
authors: H
authors: Sato
authors: Tsukasa
authors: T
authors: Matsuda
authors: Tatsuya
authors: T
authors: Morita
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D001467:Hordeum / Q000737:chemistry*; D007275:Injections, Intravenous; D037181:Lectins, C-Type / Q000737:chemistry* / Q000235:genetics; D057230:Limit of Detection; D008297:Male; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D047071:beta-Glucans / Q000097:blood* / Q000493:pharmacokinetics; D000099181:Dectin-1
chemical_list: D037181:Lectins, C-Type; D011994:Recombinant Proteins; D047071:beta-Glucans; D000099181:Dectin-1