title: Activity-guided isolation of cytotoxic bis-bibenzyl constituents from Dumortiera hirsuta.
abstract: Activity-guided fractionation of the ether extract of Dumortiera hirsute (Japanese liverwort), using cytotoxicity testing with cultured HL 60 and KB cells, resulted in the isolation of a new cytotoxic bis-bibenzyl compound, along with the two known bis-bibenzyls: isomarchantin C and isoriccardin C. The structural determination of the new bis-bibenzyl through extensive NMR spectral data indicated a derivative of marchantin A, which has been isolated from the liverwort Marchantia polymorpha. The cytotoxicity of the bis-bibenzyls was evaluated by the MTT (3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay using cultured HL 60 and KB cells.
authors: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan. toyota@ph.bunri-u.ac.jp
authors: Masao
authors: M
authors: Toyota
authors: Risa
authors: R
authors: Ikeda
authors: Hiromichi
authors: H
authors: Kenmoku
authors: Yoshinori
authors: Y
authors: Asakawa
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001632:Bibenzyls / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D005591:Chemical Fractionation / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018922:HL-60 Cells; D035926:Hepatophyta / Q000737:chemistry*; D006801:Humans; D007624:KB Cells; D010647:Phenyl Ethers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001632:Bibenzyls; D004988:Ethers, Cyclic; D010647:Phenyl Ethers; C459129:marchantin C; C505660:riccardin C