title: Analysis of light scattering by red blood cells in ektacytometry using global pattern fitting.
abstract: Ektacytometry measures the shape of red blood cells under shear stress by analyzing the diffraction pattern of laser light passing through a thin layer of suspended cells. Here we model the diffraction pattern using a combination of Bessel and anomalous scattering functions, and employ a global pattern-fitting technique over nine different shear stresses to determine the separate mechanical properties of normal and non-deformable cells. This technique is capable of yielding the correct elongation index of the normal cells over a range of shear stresses even when they are mixed with as much as 50% non-deformable cells. Additionally, the relative concentrations of normal and non-deformable cells can be determined.
authors: Department of Medicine, University of Pécs, Pécs, Hungary.
authors: Miklos
authors: M
authors: Rabai
authors: Herbert J
authors: HJ
authors: Meiselman
authors: Rosalinda B
authors: RB
authors: Wenby
authors: Jon A
authors: JA
authors: Detterich
authors: Jack
authors: J
authors: Feinberg
mesh_terms: D000328:Adult; D003198:Computer Simulation; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000737:chemistry / Q000502:physiology*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008027:Light; D008962:Models, Theoretical; D012542:Scattering, Radiation; D033081:Shear Strength
chemical_list: D005976:Glutaral
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