title: Immunotherapeutic effects of recombinant adenovirus encoding granulocyte-macrophage colony-stimulating factor in experimental pulmonary tuberculosis.
abstract: BALB/c mice with pulmonary tuberculosis (TB) develop a T helper cell type 1 that temporarily controls bacterial growth. Bacterial proliferation increases, accompanied by decreasing expression of interferon (IFN)-γ, tumour necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS). Activation of dendritic cells (DCs) is delayed. Intratracheal administration of only one dose of recombinant adenoviruses encoding granulocyte-macrophage colony-stimulating factor (AdGM-CSF) 1 day before Mycobacterium tuberculosis (Mtb) infection produced a significant decrease of pulmonary bacterial loads, higher activated DCs and increased expression of TNF-α, IFN-γ and iNOS. When AdGM-CSF was given in female mice B6D2F1 (C57BL/6J X DBA/2J) infected with a low Mtb dose to induce chronic infection similar to latent infection and corticosterone was used to induce reactivation, a very low bacilli burden in lungs was detected, and the same effect was observed in healthy mice co-housed with mice infected with mild and highly virulent bacteria in a model of transmissibility. Thus, GM-CSF is a significant cytokine in the immune protection against Mtb and gene therapy with AdGM-CSF increased protective immunity when administered in a single dose 1 day before Mtb infection in a model of progressive disease, and when used to prevent reactivation of latent infection or transmission.
authors: Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico.
authors: A
authors: A
authors: Francisco-Cruz
authors: D
authors: D
authors: Mata-Espinosa
authors: S
authors: S
authors: Estrada-Parra
authors: Z
authors: Z
authors: Xing
authors: R
authors: R
authors: Hernández-Pando
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000276:immunology*; D007167:Immunotherapy; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D014397:Tuberculosis, Pulmonary / Q000235:genetics / Q000276:immunology / Q000628:therapy* / Q000635:transmission
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor
references: J Immunol. 2011 Nov 15;187(10):5346-56
references: 22003204
references: Nat Rev Immunol. 2001 Oct;1(1):20-30
references: 11905811
references: Mol Ther. 2008 Jun;16(6):1065-72
references: 18431363
references: Curr Opin Immunol. 1995 Aug;7(4):512-6
references: 7495515
references: Antimicrob Agents Chemother. 2008 Feb;52(2):716-8
references: 17984233
references: Lancet. 1997 May 24;349(9064):1513-5
references: 9167461
references: Immunology. 2004 Aug;112(4):661-8
references: 15270738
references: Immunology. 1996 Sep;89(1):26-33
references: 8911136
references: N Engl J Med. 2001 Oct 11;345(15):1098-104
references: 11596589
references: Blood. 2000 Apr 1;95(7):2337-45
references: 10733504
references: Eur J Immunol. 2010 Jan;40(1):153-61
references: 19830735
references: Nat Rev Immunol. 2008 Jul;8(7):533-44
references: 18551128
references: J Immunol. 2002 Sep 15;169(6):2875-85
references: 12218100
references: J Immunol. 2002 Dec 1;169(11):6324-31
references: 12444139
references: Lancet Infect Dis. 2003 Sep;3(9):578-90
references: 12954564
references: Nat Immunol. 2011 Mar;12(3):231-8
references: 21240265
references: Cell. 1987 Nov 20;51(4):675-86
references: 3499986
references: J Biol Chem. 1977 Mar 25;252(6):1998-2003
references: 300377
references: Am Rev Respir Dis. 1992 Nov;146(5 Pt 1):1279-86
references: 1443885
references: AIDS. 2001 Jan 26;15(2):215-22
references: 11216930
references: J Infect Dis. 2000 Jan;181(1):390-4
references: 10608795
references: Am J Physiol Lung Cell Mol Physiol. 2002 Sep;283(3):L541-8
references: 12169573
references: Mol Ther. 2008 Jun;16(6):1161-9
references: 18388911
references: Cytokine. 1993 Jan;5(1):47-56
references: 8485305
references: J Immunol. 2007 Dec 15;179(12):8418-24
references: 18056388
references: Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2568-73
references: 20133793
references: J Acquir Immune Defic Syndr. 2007 Mar 1;44(3):254-61
references: 17211283
references: Emerg Infect Dis. 2006 Sep;12(9):1383-8
references: 17073087
references: Cochrane Database Syst Rev. 2010 Jan 20;(1):CD000171
references: 20091503
references: Immunol Cell Biol. 2001 Aug;79(4):376-84
references: 11488985
references: Emerg Infect Dis. 2006 May;12(5):744-51
references: 16704830
references: Immunology. 1997 Apr;90(4):607-17
references: 9176116
references: Cell Res. 2006 Feb;16(2):126-33
references: 16474424
references: Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5592-6
references: 8202532
references: MMWR Recomm Rep. 2009 Apr 10;58(RR-4):1-207; quiz CE1-4
references: 19357635
references: Immunology. 2009 Sep;128(1):123-33
references: 19191912
references: Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12557-61
references: 9356488
references: Immunol Cell Biol. 2010 Oct;88(7):716-26
references: 20212510
references: Int J Cell Cloning. 1990 Jan;8 Suppl 1:35-44; discussion 44-5
references: 2157777
references: Haematologica. 2010 Nov;95(11):1823-31
references: 20595096
references: Cochrane Database Syst Rev. 2000;(2):CD001363
references: 10796642
references: Clin Exp Immunol. 2002 May;128(2):229-37
references: 11985512
references: Am J Respir Crit Care Med. 1998 Oct;158(4):1156-62
references: 9769275
references: Clin Exp Immunol. 1998 Aug;113(2):157-65
references: 9717963
references: N Engl J Med. 2011 Jul 7;365(1):21-31
references: 21732834
references: Trends Microbiol. 1998 Mar;6(3):107-12
references: 9582936
references: J Leukoc Biol. 2005 Jun;77(6):914-22
references: 15767289
references: Arch Iran Med. 2006 Jul;9(3):208-12
references: 16859052
references: Immunity. 2008 Feb;28(2):271-84
references: 18261937
references: Vaccine. 2007 Jan 26;25(7):1342-52
references: 17052817
references: Cytokine. 1996 Jun;8(6):501-6
references: 8818548
references: Am J Respir Crit Care Med. 2010 Jun 15;181(12):1345-54
references: 20167854