title: The MYB oncogene can suppress apoptosis in acute myeloid leukemia cells by transcriptional repression of DRAK2 expression.
abstract: RNA interference-mediated suppression of MYB expression promoted apoptosis in the AML cell line U937, without affecting expression of the anti-apoptotic MYB target BCL2. This was accompanied by up-regulation of the pro-apoptotic gene DRAK2 and stimulation of caspase-9 activity. Moreover, RNA interference-mediated suppression of DRAK2 in U937 cells alleviated apoptosis induced by MYB down-regulation. Finally ChIP assays showed that in U937 cells MYB binds to a conserved element upstream of the DRAK2 transcription start site. Together, these findings identify a novel mechanism by which MYB suppresses apoptosis in an AML model cell line.
authors: School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia.
authors: Ping
authors: P
authors: Ye
authors: Liang
authors: L
authors: Zhao
authors: Thomas J
authors: TJ
authors: Gonda
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020626:Oncogene Proteins v-myb / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D014158:Transcription, Genetic; D020298:U937 Cells
chemical_list: D051017:Apoptosis Regulatory Proteins; D020626:Oncogene Proteins v-myb; D019253:Proto-Oncogene Proteins c-bcl-2; D017346:Protein Serine-Threonine Kinases; C115489:STK17B protein, human; D053453:Caspase 9