title: Assessment of SNaPshot and single step RT-qPCR methods for discriminating Potato virus Y (PVY) subgroups.
abstract: Potato virus Y (PVY) is the most important virus infecting potato (Solanum tuberosum), causing potato tuber necrotic ringspot disease (PTNRD), with a great impact on seed potato production. Numerous PVY strain groups with different pathogenicity and economical impact are distributed worldwide. Tools for accurate and reliable detection and discrimination of PVY strain groups are therefore essential for successful disease management. Two state of the art characterization tools based on detecting molecular markers - RT-qPCR (Kogovsek et al., 2008) and SNaPshot (Rolland et al., 2008) - were assessed for their ability to assign PVY accurately to the correct group. The results were validated by bioassay, ELISA and in silico sequence analysis. The spectrum of PVY strain groups distinguished by SNaPshot is broader than that by RT-qPCR. However, the latter was more reliable in discriminating the PVY(NTN) group members, known for their ability to induce PTNRD on selected potato cultivars. The difference in discrimination precision was due to different molecular markers being targeted by RT-qPCR and SNaPshot. Both tools use genotypic markers for detecting PVY(NTN) strain groups. Future development, however, should be focused on identifying the genomic determinants of the tuber necrosis property. Until then, the RT-qPCR and SNaPshot methods remain the most powerful diagnostic tools for detecting the PVY subgroup isolates found in Europe.
authors: National Institute of Biology, Department of Biotechnology and Systems Biology, Večna pot 111, 1000 Ljubljana, Slovenia. matevz.rupar@nib.si
authors: Matevž
authors: M
authors: Rupar
authors: Polona
authors: P
authors: Kogovšek
authors: Maruša
authors: M
authors: Pompe-Novak
authors: Ion
authors: I
authors: Gutiérrez-Aguirre
authors: Agnes
authors: A
authors: Delaunay
authors: Emmanuel
authors: E
authors: Jacquot
authors: Maja
authors: M
authors: Ravnikar
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D011198:Solanum tuberosum / Q000821:virology*
chemical_list: D012367:RNA, Viral