title: Fluorescence-activated cell sorting-based quantitation of T cell receptor restimulation-induced cell death in activated, primary human T cells.
abstract: After initial stimulation with antigen and exposure to the growth cytokine interleukin-2, activated T lymphocytes become sensitized to apoptosis upon antigen restimulation through the T cell receptor. This self-regulatory, restimulation-induced cell death (RICD) program constrains the proliferative capacity of activated T cells to help prevent excessive T cell accumulation and associated immunopathology. Here we describe a simple FACS-based approach for measuring RICD sensitivity in activated human T cells following polyclonal restimulation in vitro. This procedure is a straightforward research and clinical diagnostic tool for assessing RICD sensitivity for T cells derived from normal donors and patients suffering from diseases causing dysregulated T cell homeostasis.
authors: Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
authors: Gil
authors: G
authors: Katz
authors: Andrew L
authors: AL
authors: Snow
mesh_terms: D017209:Apoptosis; D018929:Cell Culture Techniques; D005434:Flow Cytometry / Q000379:methods*; D006706:Homeostasis; D006801:Humans; D008213:Lymphocyte Activation; D011419:Propidium / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*
chemical_list: D011948:Receptors, Antigen, T-Cell; D011419:Propidium