title: A new arabinoxylan from green leaves of Litsea glutinosa (Lauraeae): structural and biological studies.
abstract: A water soluble new arabinoxylan, isolated through hot water extraction from the green leaves of Litsea glutinosa (Lauraceae) was found to contain xylose and arabinose in a molar ratio of nearly 1:3. On the basis of NMR ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMBC and DEPT-135), GLC and GLC-MS analyses, the backbone was established as (1→4)-α-D-xylopyranosyl residue, substituted at C-2 with one unit of two adjacently linked (1→3)-α-L-arabinofuranosyl residues and the other one was terminated by β-L-arabinofuranosyl residue. The proposed repeating unit of the molecule was established as: [formula, see text] This molecule showed strong splenocyte, thymocyte, and macrophage activations. The optimum doses of the polysaccharide for splenocyte and thymocyte proliferation were observed at 25 μg/mL and 50 μg/mL, respectively. An enhanced production of NO was observed at 100 μg/mL of the polysaccharide.
authors: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Debsankar
authors: D
authors: Das
authors: Swatilekha
authors: S
authors: Maiti
authors: Tapas K
authors: TK
authors: Maiti
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D007536:Isomerism; D032862:Litsea / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects; D014867:Water / Q000737:chemistry; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014990:Xylans; D014867:Water; D009569:Nitric Oxide; C085118:arabinoxylan