title: High pleural ammonia negatively interferes with the measurement of adenosine deaminase activity.
abstract: Pleural adenosine deaminase activity (ADA) is a sensitive and specific test for tuberculous pleurisy. Here, we report a case of undetectable ADA in the pleural fluid of a man presenting with chronic cough, fever and night sweats. Subsequent laboratory investigations and review revealed that the presence of high concentrations of ammonia in pleural fluid, commonly seen in empyema, negatively interferes with ADA results when measured by the Guisti and Galanti method. The source of ammonia may come from deamination of amino acids, ammonia-producing microbes and/or leucocytes. This interference invalidates ADA results and is present in ∼2% of our laboratory requests. It is important to keep this interference in mind when tuberculosis/ammonia-producing bacteria coinfections are suspected and during early phases of tuberculous pleurisy, when neutrophils predominate in the pleural fluids.
authors: Department of Laboratory Medicine, National University Hospital, Singapore, Singapore. lohtp@yahoo.com
authors: Tze Ping
authors: TP
authors: Loh
authors: Karen Mei Ling
authors: KM
authors: Tan
authors: Suru
authors: S
authors: Chew
authors: Douglas S G
authors: DS
authors: Chan
mesh_terms: D000243:Adenosine Deaminase; D000641:Ammonia; D004796:Clinical Enzyme Tests; D003951:Diagnostic Errors; D005122:Exudates and Transudates / Q000737:chemistry / Q000201:enzymology*; D006801:Humans; D008297:Male; D014396:Tuberculosis, Pleural / Q000175:diagnosis*
chemical_list: D000641:Ammonia; D000243:Adenosine Deaminase
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