title: Characterization of peptides obtained from digests of bovine brain which accelerate structural conversions of the recombinant bovine prion protein.
abstract: Structural changes of proteins are thought to involve specific protein-peptide interactions, thus we hypothesize that certain peptides may contribute to the conformational change of prion proteins. Hence peptide libraries were constructed from partial digests of bovine brain. Using a recently developed conversion assay method, we have screened peptides responsible for structural conversion. Positive components were identified of which amino acid sequences were elucidated by top-down sequencing using mass spectrometry. A database search identified a peptide derived from synaptophysin. This peptide was chemically synthesized to confirm acceleration of the structural change of recombinant bovine prion protein.
authors: HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto 602-8158, Japan. noki@hipep.jp
authors: Kiyoshi
authors: K
authors: Nokihara
authors: Shunsuke
authors: S
authors: Yajima
authors: Akiyoshi
authors: A
authors: Hitara
authors: Tetsuya
authors: T
authors: Sogon
authors: Tadashi
authors: T
authors: Yasuhara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry; D019151:Peptide Library; D011328:Prions / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D020539:Sequence Analysis, Protein; D016708:Synaptophysin / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D019151:Peptide Library; D011328:Prions; D011994:Recombinant Proteins; D016708:Synaptophysin