title: Alterations of ultrastructural and fission/fusion markers in hepatocyte mitochondria from mice following calorie restriction with different dietary fats.
abstract: We analyzed ultrastructural changes and markers of fission/fusion in hepatocyte mitochondria from mice submitted to 40% calorie restriction (CR) for 6 months versus ad-libitum-fed controls. To study the effects of dietary fat under CR, animals were separated into three CR groups with soybean oil (also in controls), fish oil, and lard. CR induced differential changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte. The number of cristae per mitochondrion was significantly higher in all CR groups compared with controls. Proteins related to mitochondrial fission (Fis1 and Drp1) increased with CR, but no changes were detected in proteins involved in mitochondrial fusion (Mfn1, Mfn2, and OPA1). Although many of these changes could be attributed to CR regardless of dietary fat, changing membrane lipid composition by different fat sources did modulate the effects of CR on hepatocyte mitochondria.
authors: Departamento de Biologia Celular, Fisiologia e Inmunologia, Universidad de Cordoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Cordoba, Spain.
authors: Husam
authors: H
authors: Khraiwesh
authors: José A
authors: JA
authors: López-Domínguez
authors: Guillermo
authors: G
authors: López-Lluch
authors: Plácido
authors: P
authors: Navas
authors: Rafael
authors: R
authors: de Cabo
authors: Jon J
authors: JJ
authors: Ramsey
authors: José M
authors: JM
authors: Villalba
authors: José A
authors: JA
authors: González-Reyes
mesh_terms: D000818:Animals; D031204:Caloric Restriction; D004041:Dietary Fats / Q000008:administration & dosage*; D034281:Dynamins / Q000378:metabolism; D005395:Fish Oils / Q000008:administration & dosage; D020558:GTP Phosphohydrolases / Q000378:metabolism; D008136:Longevity / Q000502:physiology; D008297:Male; D008561:Membrane Fusion / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D008930:Mitochondria, Liver / Q000378:metabolism* / Q000648:ultrastructure*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D013024:Soybean Oil / Q000008:administration & dosage
chemical_list: D004041:Dietary Fats; C561064:FIS1 protein, mouse; D005395:Fish Oils; D024101:Mitochondrial Proteins; D013024:Soybean Oil; D020558:GTP Phosphohydrolases; C488750:Mfn1 protein, mouse; C488751:Mfn2 protein, mouse; C498809:Opa1 protein, mouse; C486391:Dnm1l protein, mouse; D034281:Dynamins; C029310:lard
keywords: Calorie restriction; Dietary fat; Hepatocyte; Mice; Mitochondrial fission and fusion; Mitochondrial ultrastructure
references: Science. 2009 Jul 10;325(5937):201-4
references: 19590001
references: J Nutr Biochem. 2001 Jun;12(6):357-364
references: 11516640
references: Mech Ageing Dev. 1980 Jul;13(3):275-83
references: 7421302
references: Ann N Y Acad Sci. 2011 Jan;1215:138-43
references: 21261652
references: Cell. 2006 Jun 30;125(7):1241-52
references: 16814712
references: J Exp Biol. 2003 Jul;206(Pt 14):2303-11
references: 12796449
references: Int J Biochem Cell Biol. 2009 Oct;41(10):2062-8
references: 19703662
references: J Nutr Sci Vitaminol (Tokyo). 2002 Aug;48(4):257-64
references: 12489815
references: J Gerontol. 1956 Jul;11(3):298-300
references: 13332224
references: Am J Physiol Endocrinol Metab. 2005 Apr;288(4):E674-84
references: 15562252
references: Ann N Y Acad Sci. 2002 Apr;959:475-90
references: 11976221
references: J Bioenerg Biomembr. 2001 Aug;33(4):279-87
references: 11710804
references: Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1768-73
references: 16446459
references: N Engl J Med. 1997 Oct 2;337(14):986-94
references: 9309105
references: Histochem Cell Biol. 2011 Oct;136(4):475-89
references: 21818579
references: Toxicol Pathol. 1995 Nov-Dec;23(6):644-52
references: 8772250
references: Biosci Rep. 2012 Dec 13;33(1):83-95
references: 23098316
references: J Gerontol A Biol Sci Med Sci. 2012 Nov;67(11):1121-31
references: 22503990
references: Am J Physiol Regul Integr Comp Physiol. 2004 Feb;286(2):R254-9
references: 14592935
references: Biofactors. 2012 Sep-Oct;38(5):349-59
references: 22730114
references: J Cell Sci. 2010 Aug 1;123(Pt 15):2533-42
references: 20940129
references: PLoS One. 2011;6(5):e20655
references: 21647385
references: Annu Rev Genet. 2012;46:265-87
references: 22934639
references: Nature. 2012 Sep 13;489(7415):318-21
references: 22932268
references: J Bioenerg Biomembr. 2003 Apr;35(2):169-79
references: 12887015
references: J Bioenerg Biomembr. 2005 Apr;37(2):83-90
references: 15906153
references: Mol Cell Biochem. 2004 Jan-Feb;256-257(1-2):331-9
references: 14977192
references: Mech Ageing Dev. 1994 Oct 20;76(2-3):215-24
references: 7885066
references: Comp Hepatol. 2010 Feb 23;9:5
references: 20178596
references: Nutrition. 2002 Jan;18(1):60-5
references: 11827767
references: Aging Cell. 2012 Feb;11(1):150-61
references: 22081942
references: Metabolism. 2000 Aug;49(8):977-83
references: 10954013
references: Physiol Rev. 2010 Jul;90(3):1165-94
references: 20664081
references: Nature. 2006 Nov 16;444(7117):337-42
references: 17086191
references: Exp Gerontol. 2000 May;35(3):299-305
references: 10832051
references: Exp Gerontol. 2010 Aug;45(7-8):503-11
references: 20451598
references: C R Biol. 2007 Jan;330(1):62-70
references: 17241949
references: J Electron Microsc (Tokyo). 1968;17(2):158-9
references: 4177281
references: Biochim Biophys Acta. 2012 Oct;1817(10):1833-8
references: 22409868
references: Biochim Biophys Acta. 2009 Jan;1793(1):5-19
references: 18620004
references: J Mol Cell Cardiol. 2013 Feb;55:64-72
references: 22902321
references: Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9112-6
references: 1409611
references: FASEB J. 2011 Feb;25(2):785-91
references: 21048043
references: Exp Gerontol. 2005 Aug-Sep;40(8-9):650-9
references: 16102930
references: Free Radic Biol Med. 1997;23(1):76-81
references: 9165299
references: Annu Rev Biochem. 2007;76:701-22
references: 17227225
references: Science. 1996 Jul 5;273(5271):59-63
references: 8658196
references: Am J Physiol Endocrinol Metab. 2004 May;286(5):E852-61
references: 14736705
references: J Cell Sci. 1981 Aug;50:433-48
references: 7320075
references: Exp Gerontol. 2008 Sep;43(9):813-9
references: 18662766
references: Science. 2005 Oct 14;310(5746):314-7
references: 16224023
references: J Cell Biol. 1978 Jun;77(3):887-901
references: 681456
references: J Cell Sci. 2010 Mar 15;123(Pt 6):917-26
references: 20179104
references: Obes Surg. 2007 Oct;17(10):1381-8
references: 18000736
references: Am J Physiol Endocrinol Metab. 2005 Sep;289(3):E429-38
references: 15886224
references: Biosci Rep. 1982 Dec;2(12):1003-7
references: 7165791
references: J Ultrastruct Mol Struct Res. 1989 Apr;102(1):87-94
references: 2621378