title: A novel oriented immobilized lipase on magnetic nanoparticles in reverse micelles system and its application in the enrichment of polyunsaturated fatty acids.
abstract: A novel oriented immobilized lipase was derived from Yarrowia lipolytica lipase LIP2 covalently immobilized on functionalized Fe3O4 magnetic nanoparticles (MNPs) in reverse micelles system (RMS). The activity recovery reached 382% compared with 29% in aqueous phase, and further ran up to 1425% under optimum conditions. (3-Aminopropyl) triethoxysilane (APTES) coated Fe3O4 nanoparticles were characterized by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD). A significant alteration in the secondary structure of the lipase in RMS with a 15.5% increase of α-helix content and a 12.5% decrease of β-sheet content was detected by circular dichroism (CD). The immobilized lipase was employed to enrich polyunsaturated fatty acids in fish oil, a 90% increase of DHA content was obtained after 12h, and after 20 cycles of successive usage, it still remained over 80% of relative hydrolysis degree, which shows a good recyclability.
authors: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors: Tao
authors: T
authors: Liu
authors: Yuandi
authors: Y
authors: Zhao
authors: Xiaofeng
authors: X
authors: Wang
authors: Xiang
authors: X
authors: Li
authors: Yunjun
authors: Y
authors: Yan
mesh_terms: D002942:Circular Dichroism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008823:Micelles; D011437:Propylamines; D011487:Protein Conformation; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction; D025062:Yarrowia / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D005231:Fatty Acids, Unsaturated; D058185:Magnetite Nanoparticles; D008823:Micelles; D011437:Propylamines; D012821:Silanes; D008049:Lipase; C477625:amino-propyl-triethoxysilane