title: Influence of pesticide exposure on carbonic anhydrase II from sheep stomach.
abstract: Carbonic anhydrase (CA) is a widely distributed enzyme and has a crucial role in the cells, tissues and organs of living organisms. It is found that CA-II is one of the most abundant CA isoenzymes in the gastrointestinal system. It plays an important role in the gastric acid secretion in stomach. In this study, we purified CA-II isoenzyme from sheep stomach with a 615.2 purification fold, 78% purification yield and 5562.02 specific activity. Moreover, the in vitro effects of some commonly used pesticides including chlorpyrifos, cypermethrin, dichlorvos, glyphosate isopropylamine and lambda cyhalomethrin on the enzyme activity were investigated. Of these compounds, glyphosate isopropylamine and dichlorvos showed an inhibition on CA-II esterase activity. They have IC50 values of 0.155 µM and 2.690 µM and Ki values of 0.329 µM and 3.654 µM, respectively. Both glyphosate isopropylamine and dichlorvos inhibited CA-II isoenzyme in a noncompetitive manner.
authors: Biochemistry Division, Faculty of Sciences, Department of Chemistry, Atatürk University, Erzurum, Turkey.
authors: Namık
authors: N
authors: Kılınç
authors: Biochemistry Division, Faculty of Sciences, Department of Chemistry, Atatürk University, Erzurum, Turkey.
authors: Mehmet Mustafa
authors: MM
authors: İşgör
authors: Biochemistry Division, Faculty of Sciences, Department of Chemistry, Atatürk University, Erzurum, Turkey.
authors: Bülent
authors: B
authors: Şengül
authors: Biochemistry Division, Faculty of Sciences, Department of Chemistry, Atatürk University, Erzurum, Turkey beydemir@atauni.edu.tr.
authors: Şükrü
authors: Ş
authors: Beydemir
mesh_terms: D000818:Animals; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002257:Carbonic Anhydrase Inhibitors / Q000633:toxicity; D004390:Chlorpyrifos / Q000633:toxicity; D004006:Dichlorvos / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity; D012756:Sheep; D013270:Stomach / Q000187:drug effects* / Q000201:enzymology
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D010575:Pesticides; D011722:Pyrethrins; C017160:cypermethrin; D004006:Dichlorvos; D024402:Carbonic Anhydrase II; D004390:Chlorpyrifos
keywords: Carbonic anhydrase; enzyme inhibition; pesticides; sheep stomach