title: Poly(amido amine) is an ideal carrier of miR-7 for enhancing gene silencing             effects on the EGFR pathway in U251 glioma cells.
abstract: microRNAs are regarded as promising drugs for glioma gene therapy. However,             conventional administration routes, such as oral administration and intravenous             infusion, present low efficiency due to the blood-brain barrier and intercellular             retention, thereby limiting their application. Recent studies showed poly(amido             amine) (PAMAM) was a candidate carrier due to its high solubilization, delayed             release and low toxicity. In the present study, U251 human brain glioma cells             were transfected with the miR-7 gene using PAMAM as the vector to determine the             transfection efficiency and therapeutic effects in vivo and in vitro. We found             that PAMAM exhibited higher transfection efficiency and longer duration of action             compared with liposome delivery, and miR-7 efficiently silenced some genes involved             in the epidermal growth factor receptor (EGFR) pathway and achieved favorable             effects in treating glioma in vivo and in vitro. These investigations provide             a basis for developing high-efficiency micromolecular drug delivery.
authors: Department of Neurosurgery, The Fifth Central Hospital of Tianjin, Tianjin, PR China.
authors: Xiaozhi
authors: X
authors: Liu
authors: Gang
authors: G
authors: Li
authors: Zhiguo
authors: Z
authors: Su
authors: Zhongmin
authors: Z
authors: Jiang
authors: Lei
authors: L
authors: Chen
authors: Junfei
authors: J
authors: Wang
authors: Shizhu
authors: S
authors: Yu
authors: Zhenlin
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001932:Brain Neoplasms / Q000188:drug therapy / Q000235:genetics; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000235:genetics*; D020868:Gene Silencing; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics*; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology; D015398:Signal Transduction
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D066246:ErbB Receptors; D008081:Liposomes; D035683:MicroRNAs; D011073:Polyamines; C549242:MIRN7-1 microRNA, human; C531249:Poly(amidoamine); C512478:EGFR protein, human