title: Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB.
abstract: Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease and may lead to joint damage, synovial membrane destruction and cartilage and bone damage. RA is closely associated with increased expression of interleukin (IL)-6, IL-8, tumor necrosis factor-α (TNF-α) and nuclear transcription factor-κB (NF-κB). Therefore, inhibition of the expression of IL-6, IL-8, TNF-α and NF-κB is a promising strategy for the development of novel anti-RA therapies. The aim of this study was to investigate the effect of shuangtengbitong tincture (STBT) on the expression of IL-6, IL-8, TNF-α and NF-κB in synovial tissues of rats with collagen-induced arthritis (CIA). STBT as a clinical prescription created at Fujian University of Traditional Chinese Medicines (TCM) Affiliated People's Hospital has been shown to be clinically effective in the treatment of RA. The model of Wistar rats with CIA was created using bovine type II collagen. The two treatment groups with CIA were administered STBT (1 ml per time) or Votalin (∼1 cm per time) for ∼1 month continuously. Following treatment, STBT suppressed paw swelling significantly (P<0.05) compared with the model group. STBT also improved pathological changes, STBT-treated rats showed a significant improvement in synovial hyperplasia, inflammatory infiltration, cartilage and bone destruction and other symptoms. The protein expression levels of IL-6, IL-8, TNF-α and NF-κB were markedly suppressed in synovial tissues of STBT-treated and Votalin-treated rats. Our findings demonstrate for the first time that STBT markedly reduces paw swelling, improves pathological changes and increases the expression of IL-6, IL-8, TNF-α and NF-κB in synovial tissues of CIA rats, which may partially explain the anti-RA activity of STBT.
authors: Pharmacy College; Fujian University of Traditional Chinese Medicine, Fuzhou 350122,  P.R. China.
authors: Kedan
authors: K
authors: Chu
authors: Haiyin
authors: H
authors: Zheng
authors: Huang
authors: H
authors: Li
authors: Yuqin
authors: Y
authors: Zhang
authors: Xun
authors: X
authors: Zhang
authors: Wei
authors: W
authors: Xu
authors: Lidian
authors: L
authors: Chen
keywords: interleukin-6; interleukin-8; nuclear transcription factor-κB; rheumatoid arthritis; shuangtengbitong tincture; tumor necrosis factor-α
references: J Exp Med. 1985 Dec 1;162(6):2163-8
references: 2999289
references: Rheumatology (Oxford). 2000 Jun;39 Suppl 1:3-8
references: 11001373
references: J Huazhong Univ Sci Technolog Med Sci. 2005;25(5):543-5
references: 16463669
references: Oncogene. 2006 Oct 30;25(51):6680-4
references: 17072321
references: J Ethnopharmacol. 2010 Feb 3;127(2):264-73
references: 19914365
references: Arthritis Rheum. 2003 Jun;48(6):1530-42
references: 12794820
references: Springer Semin Immunopathol. 2003 Aug;25(1):3-18
references: 12904888
references: Nat Rev Immunol. 2002 Jul;2(7):527-35
references: 12094227
references: Ann Rheum Dis. 1995 Jan;54(1):33-8
references: 7880119
references: J Immunol. 2005 Jun 15;174(12):7894-903
references: 15944295
references: J Ethnopharmacol. 2008 Aug 13;118(3):479-84
references: 18577440
references: Annu Rev Med. 2000;51:207-29
references: 10774461
references: Calcif Tissue Int. 2008 Nov;83(5):354-64
references: 18931819
references: Nat Rev Immunol. 2007 Jun;7(6):429-42
references: 17525752
references: Arthritis Rheum. 1992 Oct;35(10):1170-8
references: 1329775
references: Int Immunopharmacol. 2010 Jan;10(1):1-8
references: 19804846
references: Cancer. 2000 Dec 15;89(12):2637-45
references: 11135226
references: Int J Biochem Cell Biol. 2004 Mar;36(3):372-8
references: 14687914
references: J Clin Invest. 2008 Nov;118(11):3537-45
references: 18982160
references: Nature. 1986 Feb 6-12;319(6053):516-8
references: 3511389
references: Cell Biol Toxicol. 2008 Jun;24(3):225-31
references: 17701078
references: Nat Prod Res. 2009;23(18):1699-706
references: 19921588
references: Arthritis Rheum. 1998 Aug;41(8):1378-87
references: 9704635