title: Preparation and investigation of LC packing made by microwave-assisted solid-phase synthesis.
abstract: The preparation of the so-called bonded phase liquid chromatographic packings is usually carried out by heating the silica, the silane, a catalyst, or a scavenger in an appropriate solvent (often toluene.) Due to the longtime of boiling, the procedure is time and energy consuming, and solvent intensive. The goal of this work is to present a simple, environment-friendly preparation method with reduced solvent consumption to synthetize RP liquid chromatographic stationary phases. The effects of reaction conditions (amount of reagents, composition of the reagent, microwave energy, reaction time, reproducibility of the synthesis) are discussed. Pore structure, surface coverage, the change of the pore structure and surface coverage upon reaction are demonstrated, the efficiency of the column (van Deemter plot for different solutes) is presented. A variety of applications (aromatic hydrocarbons, halobenzenes, bioactive peptides, resveratrol from red wine) demonstrates the separation power of the new phase.
authors: Institute of Biochemistry and Medical Chemistry, Medical School, University of Pecs, Pecs, Hungary.
authors: Eva
authors: E
authors: Jambor
authors: Agnes
authors: A
authors: Bona
authors: Janos
authors: J
authors: Schmidt
authors: Laszlo
authors: L
authors: Mark
authors: Robert
authors: R
authors: Ohmacht
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D008872:Microwaves; D010455:Peptides / Q000032:analysis*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry; D000077185:Resveratrol; D060327:Solid-Phase Synthesis Techniques; D013267:Stilbenes / Q000032:analysis*; D014920:Wine / Q000032:analysis
chemical_list: D010455:Peptides; D011108:Polymers; D012117:Resins, Synthetic; D013267:Stilbenes; D000077185:Resveratrol