title: Signal recognition initiates reorganization of the presequence translocase during protein import.
abstract: The mitochondrial presequence translocase interacts with presequence-containing precursors at the intermembrane space (IMS) side of the inner membrane to mediate their translocation into the matrix. Little is known as too how these matrix-targeting signals activate the translocase in order to initiate precursor transport. Therefore, we analysed how signal recognition by the presequence translocase initiates reorganization among Tim-proteins during import. Our analyses revealed that the presequence receptor Tim50 interacts with Tim21 in a signal-sensitive manner in a process that involves the IMS-domain of the Tim23 channel. The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport.
authors: Department of Biochemistry II, University of Göttingen, Göttingen, Germany.
authors: Oleksandr
authors: O
authors: Lytovchenko
authors: Jonathan
authors: J
authors: Melin
authors: Christian
authors: C
authors: Schulz
authors: Markus
authors: M
authors: Kilisch
authors: Dana P
authors: DP
authors: Hutu
authors: Peter
authors: P
authors: Rehling
mesh_terms: D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D008958:Models, Molecular; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs / Q000235:genetics / Q000502:physiology; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D017433:Protein Structure, Secondary; D021381:Protein Transport / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D013379:Substrate Specificity
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; C503873:Pam17 protein, S cerevisiae; D021382:Protein Sorting Signals; D029701:Saccharomyces cerevisiae Proteins; C083081:TIM23 protein, S cerevisiae; C469615:TIM50 protein, S cerevisiae; C500397:Tim21 protein, S cerevisiae
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