title: Complete nucleotide sequences of plasmids pACK2 and pACK5 from Staphylococcus simulans biovar staphylolyticus.
abstract: Staphylococcus simulans biovar staphylolyticus contains five plasmids, designated pACK1-pACK5. Recently, the nucleotide sequences of three of these plasmids, pACK1, pACK3, and pACK4, were reported. In order to complete the characterization of these five plasmids, the nucleotide sequences of the two remaining plasmids, pACK2 (37683 bp) and pACK5 (3191 bp), were determined. pACK5 is comprised of two regions, one with 85% identity at the nucleotide level to a region of pWBG1773 and another region with an ORF that shares no significant similarity to sequences previously described in GenBank. pACK2 encodes proteins for cadmium resistance and enhanced biofilm formation. The similarities at the nucleotide level among regions of the plasmids of S. simulans bv. staphylolyticus suggest that these plasmids have undergone multiple intermolecular rearrangements.
authors: Department of Biological Sciences, The University of Alabama, Tuscaloosa, AL 35487-0334, USA. asgargis@gmail.com
authors: Amy S
authors: AS
authors: Gargis
authors: Lucie S
authors: LS
authors: Heath
authors: Harry E
authors: HE
authors: Heath
authors: Paul A
authors: PA
authors: Leblanc
authors: Shaw R
authors: SR
authors: Gargis
authors: Travis H
authors: TH
authors: Harris
authors: Gary L
authors: GL
authors: Sloan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D018441:Biofilms; D019187:Cadmium Compounds / Q000494:pharmacology; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D015321:Gene Rearrangement; D016366:Open Reading Frames; D009876:Operon; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin; D012689:Sequence Homology, Nucleic Acid; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013431:Sulfates / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D019187:Cadmium Compounds; D004269:DNA, Bacterial; D013431:Sulfates; C037123:cadmium sulfate