title: Validation of an antiviral assay method for quantifying IFN-α5 activity in macaque and human serum.
abstract: BACKGROUND
  IFN-α5 has been demonstrated to induce stronger signaling and higher expression of antiviral genes than IFN-α2, which is the current treatment in chronic viral hepatitis. However, there is no specific and validated quantification method in order to conduct kinetic studies as part of the preclinical and clinical evaluation for regulatory purposes.
  
  
  RESULTS
  A novel integration of an antiviral assay against the cytopathic effect of the encephalomyocarditis virus in HeLa cells with a very sensitive method for assay processing - the Vialight(®) Plus assay - is presented for IFN-α5 activity quantification. The bioassay has been validated in macaque and human serum and it has been demonstrated to be selective, precise and accurate.
  
  
  CONCLUSIONS
  The validated bioassay meets suitable acceptance criteria for these types of biological assays.
authors: Toxicology Unit, Center for Applied Pharmacobiology Research, University of Navarra, Irunlarrea 1, E-31008 Pamplona, Spain. larbillaga@unav.es
authors: Leire
authors: L
authors: Arbillaga
authors: Mayte
authors: M
authors: Murillo-Arbizu
authors: Iranzu
authors: I
authors: González
authors: Ana G
authors: AG
authors: Gil
authors: Ariane
authors: A
authors: Vettorazzi
authors: Elena
authors: E
authors: González-Peñas
authors: Esther
authors: E
authors: Larrea
authors: Adela López
authors: AL
authors: de Cerain
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods / Q000592:standards; D018188:Cardiovirus Infections / Q000097:blood; D003588:Cytopathogenic Effect, Viral; D004686:Encephalomyocarditis virus / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D016898:Interferon-alpha / Q000097:blood*; D008251:Macaca
chemical_list: D016898:Interferon-alpha