title: Insecticidal and genotoxic activity of Psoralea corylifolia Linn. (Fabaceae) against Culex quinquefasciatus Say, 1823.
abstract: BACKGROUND
  Indiscriminate use of synthetic insecticides to eradicate mosquitoes has caused physiological resistance. Plants provide a reservoir of biochemical compounds; among these compounds some have inhibitory effect on mosquitoes. In the present study the larvicidal, adulticidal and genotoxic activity of essential oil of Psoralea corylifolia Linn. against Culex quinquefasciatus Say was explored.
  
  
  METHODS
  Essential oil was isolated from the seeds of P. corylifolia Linn. Larvicidal and adulticidal bioassay of Cx. quinquefasciatus was carried out by WHO method. Genotoxic activity of samples was determined by comet assay. Identification of different compounds was carried out by gas chromatography- mass spectrometry analysis.
  
  
  RESULTS
  LC50 and LC90 values of essential oil were 63.38±6.30 and 99.02±16.63 ppm, respectively against Cx. quinquefasciatus larvae. The LD50 and LD90 values were 0.057±0.007 and 0.109±0.014 mg/cm2 respectively against adult Cx. quinquefasciatus,. Genotoxicity of adults was determined at 0.034 and 0.069 mg/cm2. The mean comet tail length was 6.2548±0.754 μm and 8.47±0.931 μm and the respective DNA damage was significant i.e. 6.713% and 8.864% in comparison to controls. GCMS analysis of essential oil revealed 20 compounds. The major eight compounds were caryophyllene oxide (40.79%), phenol,4-(3,7-dimethyl-3-ethenylocta-1,6-dienyl) (20.78%), caryophyllene (17.84%), α-humulene (2.15%), (+)- aromadendrene (1.57%), naphthalene, 1,2,3,4-tetra hydro-1,6-dimethyle-4-(1-methyl)-, (1S-cis) (1.53%), trans- caryophyllene (0.75%), and methyl hexadecanoate (0.67%).
  
  
  CONCLUSIONS
  Essential oil obtained from the seeds of P. corylifolia showed potent toxicity against larvae and adult Cx. quinquefasciatus. The present work revealed that the essential oil of P. corylifolia could be used as environmentally sound larvicidal and adulticidal agent for mosquito control.
authors: National Institute of Malaria Research, Sector-3, Health Centre, Field Unit BHEL, Ranipur, Hardwar, Uttrakhand, 249403, India. vkdua51@gmail.com
authors: Virendra K
authors: VK
authors: Dua
authors: Arvind
authors: A
authors: Kumar
authors: Akhilesh C
authors: AC
authors: Pandey
authors: Sandeep
authors: S
authors: Kumar
mesh_terms: D000818:Animals; D001681:Biological Assay; D020552:Comet Assay; D003465:Culex / Q000187:drug effects* / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D009153:Mutagens / Q000302:isolation & purification / Q000494:pharmacology*; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D029907:Psoralea / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D016019:Survival Analysis
chemical_list: D007306:Insecticides; D009153:Mutagens; D009822:Oils, Volatile; D010936:Plant Extracts
references: J Am Mosq Control Assoc. 1987 Jun;3(2):302-3
references: 3333059
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jul 5;821(1):67-74
references: 15905140
references: J Am Mosq Control Assoc. 2006 Sep;22(3):515-22
references: 17067055
references: Environ Pollut. 2009 Feb;157(2):481-7
references: 18977573
references: Bioresour Technol. 2009 Jan;100(1):457-64
references: 18396039
references: Mech Ageing Dev. 2004 Jan;125(1):41-6
references: 14706236
references: J Med Entomol. 2006 Nov;43(6):1222-6
references: 17162957
references: Bioresour Technol. 2009 Jan;100(1):465-70
references: 18178080
references: J Cell Biochem. 2008 May 15;104(2):657-67
references: 18172854
references: Prog Neuropsychopharmacol Biol Psychiatry. 2008 Feb 15;32(2):510-9
references: 18006202
references: N Engl J Med. 2006 Aug 31;355(9):869-71
references: 16943399
references: Parasitol Res. 2012 Mar;110(3):1261-8
references: 21881945
references: Ann Intern Med. 1998 Jun 1;128(11):931-40
references: 9634433
references: J Am Mosq Control Assoc. 2008 Jun;24(2):315-8
references: 18666542
references: J Asian Nat Prod Res. 2007 Jan-Feb;9(1):41-4
references: 17365188
references: Indian J Med Res. 2010 Mar;131:434-9
references: 20418559
references: J Agric Food Chem. 2004 Jul 14;52(14):4395-400
references: 15237942
references: Insect Biochem Mol Biol. 1999 Oct;29(10):909-14
references: 10528410
references: Ecotoxicol Environ Saf. 2010 Sep;73(6):1415-23
references: 20627310
references: Mutat Res. 2002 Apr 26;516(1-2):148-52
references: 11943620
references: Bioresour Technol. 2005 Jul;96(11):1235-40
references: 15734310
references: Med J Armed Forces India. 2006 Oct;62(4):359-62
references: 27688542
references: Environ Monit Assess. 2009 Oct;157(1-4):97-104
references: 18758975
references: J Ethnopharmacol. 2009 Jan 21;121(2):286-91
references: 19041711
references: Chem Pharm Bull (Tokyo). 2007 Jan;55(1):106-10
references: 17202711
references: J Am Mosq Control Assoc. 2011 Sep;27(3):252-5
references: 22017089
references: Toxicol Pathol. 2002 Jul-Aug;30(4):536-8
references: 12187945
references: Bioresour Technol. 2003 Aug;89(1):99-102
references: 12676507
references: Front Biosci. 2003 May 01;8:d963-81
references: 12700077
references: Parasitol Res. 2010 Jul;107(2):327-35
references: 20405142
references: J Hist Med Allied Sci. 2011 Jul;66(3):347-76
references: 20624820
references: J Virol. 2010 Oct;84(19):9967-77
references: 20631128
references: Mutat Res. 2009 Jan-Feb;681(1):93-109
references: 19056514
references: Indian J Med Res. 2012;135:120-6
references: 22382193
references: Fitoterapia. 2000 Jun;71(3):223-31
references: 10844159
references: J Sep Sci. 2007 Apr;30(6):813-8
references: 17536725
references: Exp Cell Res. 1988 Mar;175(1):184-91
references: 3345800
references: Mutat Res. 1999 Jul 21;444(1):103-16
references: 10477344
references: Mutagenesis. 2008 May;23(3):191-205
references: 18326867
references: J Epidemiol Community Health. 2010 Feb;64(2):148-54
references: 19843497
references: Bioresour Technol. 2008 May;99(8):3251-5
references: 17662602
references: Parasitol Res. 2012 Jun;110(6):2125-30
references: 22167373
references: Bull World Health Organ. 2000;78(9):1136-47
references: 11019462
references: Chem Biodivers. 2010 Aug;7(8):2040-5
references: 20730967