title: Experimental identification of Actinobacillus pleuropneumoniae strains L20 and JL03 heptosyltransferases, evidence for a new heptosyltransferase signature sequence.
abstract: We experimentally identified the activities of six predicted heptosyltransferases in Actinobacillus pleuropneumoniae genome serotype 5b strain L20 and serotype 3 strain JL03. The initial identification was based on a bioinformatic analysis of the amino acid similarity between these putative heptosyltrasferases with others of known function from enteric bacteria and Aeromonas. The putative functions of all the Actinobacillus pleuropneumoniae heptosyltrasferases were determined by using surrogate LPS acceptor molecules from well-defined A. hydrophyla AH-3 and A. salmonicida A450 mutants. Our results show that heptosyltransferases APL_0981 and APJL_1001 are responsible for the transfer of the terminal outer core D-glycero-D-manno-heptose (D,D-Hep) residue although they are not currently included in the CAZY glycosyltransferase 9 family. The WahF heptosyltransferase group signature sequence [S(T/S)(GA)XXH] differs from the heptosyltransferases consensus signature sequence [D(TS)(GA)XXH], because of the substitution of D(261) for S(261), being unique.
authors: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Barcelona, Spain.
authors: Susana
authors: S
authors: Merino
authors: Yuriy A
authors: YA
authors: Knirel
authors: Miguel
authors: M
authors: Regué
authors: Juan M
authors: JM
authors: Tomás
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000201:enzymology* / Q000235:genetics*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019295:Computational Biology; D016695:Glycosyltransferases / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry; D009154:Mutation; D010802:Phylogeny
chemical_list: D008070:Lipopolysaccharides; D016695:Glycosyltransferases; C086735:heptosyltransferase
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