title: Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: food and biological fluids.
abstract: Sample preparation and laser-induced fluorescence detection are two key steps of the analytical methodology to determine by capillary electrophoresis low concentrations of proteins in complex sample matrices. In this chapter the options of performing both steps in different ways are shown by detailing the analysis of the allergen β-lactoglobulin in food products for infants and the analysis of the isoforms of alpha 1-acid glycoprotein, a potential biomarker, in serum and secretome.
authors: Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain.
authors: Raul
authors: R
authors: Garrido-Medina
authors: Angel
authors: A
authors: Puerta
authors: Cristina
authors: C
authors: Pelaez-Lorenzo
authors: Zuly
authors: Z
authors: Rivera-Monroy
authors: Andras
authors: A
authors: Guttman
authors: Jose Carlos
authors: JC
authors: Diez-Masa
authors: Mercedes
authors: M
authors: de Frutos
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000737:chemistry; D002846:Chromatography, Affinity; D019075:Electrophoresis, Capillary / Q000379:methods / Q000592:standards; D005456:Fluorescent Dyes / Q000737:chemistry; D005512:Food Hypersensitivity / Q000517:prevention & control; D005663:Furans / Q000737:chemistry; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D007782:Lactoglobulins / Q000032:analysis* / Q000737:chemistry; D007834:Lasers; D009961:Orosomucoid / Q000302:isolation & purification / Q000378:metabolism*; D011804:Quinolines / Q000737:chemistry; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D000485:Allergens; D005456:Fluorescent Dyes; D005663:Furans; D007782:Lactoglobulins; D009961:Orosomucoid; D011804:Quinolines; C063756:3-(2-furoyl)quinoline-2-carbaldehyde