title: Air flow-assisted ionization imaging mass spectrometry method for easy whole-body molecular imaging under ambient conditions.
abstract: Whole-body molecular imaging is able to directly map spatial distribution of molecules and monitor its biotransformation in intact biological tissue sections. Imaging mass spectrometry (IMS), a label-free molecular imaging method, can be used to image multiple molecules in a single measurement with high specificity. Herein, a novel easy-to-implement, whole-body IMS method was developed with air flow-assisted ionization in a desorption electrospray ionization mode. The developed IMS method can effectively image molecules in a large whole-body section in open air without sample pretreatment, such as chemical labeling, section division, or matrix deposition. Moreover, the signal levels were improved, and the spatial assignment errors were eliminated; thus, high-quality whole-body images were obtained. With this novel IMS method, in situ mapping analysis of molecules was performed in adult rat sections with picomolar sensitivity under ambient conditions, and the dynamic information of molecule distribution and its biotransformation was provided to uncover molecular events at the whole-animal level. A global view of the differential distribution of an anticancer agent and its metabolites was simultaneously acquired in whole-body rat and model mouse bearing neuroglioma along the administration time. The obtained drug distribution provided rich information for identifying the targeted organs and predicting possible tumor spectrum, pharmacological activity, and potential toxicity of drug candidates.
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
authors: Zhigang
authors: Z
authors: Luo
authors: Jiuming
authors: J
authors: He
authors: Yi
authors: Y
authors: Chen
authors: Jingjing
authors: J
authors: He
authors: Tao
authors: T
authors: Gong
authors: Fei
authors: F
authors: Tang
authors: Xiaohao
authors: X
authors: Wang
authors: Ruiping
authors: R
authors: Zhang
authors: Lan
authors: L
authors: Huang
authors: Lianfeng
authors: L
authors: Zhang
authors: Haining
authors: H
authors: Lv
authors: Shuanggang
authors: S
authors: Ma
authors: Zhaodi
authors: Z
authors: Fu
authors: Xiaoguang
authors: X
authors: Chen
authors: Shishan
authors: S
authors: Yu
authors: Zeper
authors: Z
authors: Abliz
mesh_terms: D000388:Air; D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D007212:Indolizines / Q000493:pharmacokinetics / Q000494:pharmacology; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D010616:Phenanthrenes; D010618:Phenanthrolines / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D051598:Whole Body Imaging / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D007212:Indolizines; D010616:Phenanthrenes; D010618:Phenanthrolines; C559326:S-(+)-deoxytylophorinidine