title: Hepatic arterial administration of ginsenoside Rg3 and transcatheter arterial embolization for the treatment of VX2 liver carcinomas.
abstract: Ginsenoside Rg3 has been demonstrated to inhibit tumor cell proliferation and angiogenesis. However, its effect on liver tumors when administered via the hepatic artery has not been investigated. The purpose of this study was to evaluate the therapeutic effect of hepatic artery administration of Rg3 combined with transcatheter arterial embolization (TAE) in the treatment of liver tumors. A total of 48 rabbits with VX2 liver tumors were randomly divided into four groups: Group 1, Rg3; Group 2, TAE; Group 3, Rg3 and TAE; and Group 4, control. Abdominal contrast computed tomography (CT) scans were performed 2 weeks before and after intervention to assess tumor growth. Immunohistochemical staining was used to detect the expression of the angiogenesis biomarkers CD31 and VEGF, and the cell apoptosis marker caspase-3. Semi-quantitative RT-PCR and western blotting were employed to detect the expression of the caspase-3, Bax and Bcl-2 apoptosis-related genes and proteins. In addition, HepG2 cells were treated with Rg3 at different concentrations (0, 25, 50, 75 and 100 mg/l) in vitro. An MTT assay and western blot analysis were used to analyze the cell proliferation and VEGF expression. Compared with the other experimental groups, the Rg3 and TAE group expressed significantly lower levels of CD31 and VEGF (P<0.05), significantly increased levels of the pro-apoptotic genes caspase-3 and Bax (P<0.05), and significantly reduced levels of anti-apoptotic Bcl-2 at the mRNA and protein levels (P<0.05). In vitro, Rg3 inhibited HepG2 cell proliferation and downregulated VEGF expression significantly. These results indicated that ginsenoside Rg3 combined with TAE may effectively inhibit tumor growth by inhibiting tumor angiogenesis and inducing cancer cell apoptosis.
authors: Departments of Gastroenterology and Hepatology, West China Hospital, Sichuan University, Chengdu 610041,  P.R. China.
authors: Yang
authors: Y
authors: Yu
authors: Chunle
authors: C
authors: Zhang
authors: Lingjun
authors: L
authors: Liu
authors: Xiao
authors: X
authors: Li
keywords: VX2 liver tumor; angiogenesis; apoptosis; ginsenoside Rg3; transcatheter arterial embolization
references: Eur J Radiol. 1999 Oct;32(1):86-9
references: 10580325
references: Arch Pharm Res. 2004 Apr;27(4):429-35
references: 15180309
references: J Exp Med. 1952 Aug;96(2):159-74
references: 14955572
references: Arch Biochem Biophys. 2002 Oct 1;406(1):1-8
references: 12234484
references: Acta Oncol. 2009;48(6):811-21
references: 19513886
references: J Gastroenterol Hepatol. 2007 Aug;22(8):1178-82
references: 17559361
references: Mutat Res. 2003 Feb-Mar;523-524:75-85
references: 12628505
references: Gut. 2001 Jan;48(1):87-96
references: 11115828
references: Int J Oncol. 1999 Jun;14(6):1087-90
references: 10339662
references: Am J Gastroenterol. 2008 Apr;103(4):914-21
references: 18177453
references: Arch Pharm Res. 2009 May;32(5):755-65
references: 19471891
references: Hepatology. 2002 May;35(5):1164-71
references: 11981766
references: J Food Sci. 2010 May;75(4):C378-82
references: 20546398
references: Acta Pharmacol Sin. 2008 Sep;29(9):1103-8
references: 18718179
references: Curr Med Chem. 2009;16(22):2924-42
references: 19689273
references: Arch Toxicol. 2008 Mar;82(3):183-95
references: 17874070
references: Biochem Pharmacol. 2006 Aug 14;72(4):437-45
references: 16793023
references: Planta Med. 2007 Jun;73(7):669-74
references: 17538869
references: J Mol Med (Berl). 2002 Nov;80(11):703-14
references: 12436347
references: Arzneimittelforschung. 1980;30(6):936-43
references: 7191267
references: Life Sci. 2000 Aug 4;67(11):1297-306
references: 10972198