title: Molecular analysis of red wine yeast diversity in the Ribera del Duero D.O. (Spain) area.
abstract: Molecular characterization of wine yeast population during spontaneous fermentation in biodynamic wines from Ribera del Duero D.O. located at northern plateau of Spain has been carried out during two consecutive years. A total of 829 yeast strains were isolated from the samples and characterized by electrophoretic karyotype. The results show the presence of three population of yeast differentiated by their electrophoretic karyotypes, (1) non-Saccharomyces yeast dominant in the initial phase of the fermentations (NS); (2) Saccharomyces bayanus var uvarum detected mainly mid-way through the fermentation process at 20-25 °C; and (3) Saccharomyces cerevisiae which remained dominant until the end of the fermentation. This is the first study showing the population dynamic of S. bayanus var. uvarum in red wines produced in Ribera del Duero that could represent an important source of autochthonous wine yeasts with novel oenological properties.
authors: Laboratorio de Microbiología y Genética, Departamento de Biomedicina, Biotecnología y Salud Pública, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Polígono del Río San Pedro s/n, 11510 Puerto Real, Cádiz, Spain.
authors: Eugenia
authors: E
authors: Muñoz-Bernal
authors: María Esther
authors: ME
authors: Rodríguez
authors: Patricia
authors: P
authors: Benítez
authors: Francisco Javier
authors: FJ
authors: Fernández-Acero
authors: Laureana
authors: L
authors: Rebordinos
authors: Jesús Manuel
authors: JM
authors: Cantoral
mesh_terms: D005285:Fermentation; D016533:Mycological Typing Techniques; D012440:Saccharomyces / Q000235:genetics / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000302:isolation & purification*; D013030:Spain; D014920:Wine / Q000382:microbiology*; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*