title: High-throughput capillary electrophoresis-mass spectrometry: from analysis of amino acids to analysis of protein complexes.
abstract: Recent advances in capillary electrophoresis-mass spectrometry (CE-MS) interfacing using porous tip is leading to commercialization of CE-MS with a sheathless interface for the first time. The new sheathless interface in conjunction with CE capillary coatings using self-coating background electrolytes (BGE) has significantly simplified CE-MS analysis of complex mixtures. CE-MS, with its high separation efficiency, compound identification capability, and ability to rapidly separate compounds with a wide range of mass and charge while consuming only nanoliters of samples, has become a valuable analytical technique for the analysis of complex biological mixtures. These advances have allowed a single capillary to analyze a range of compounds including amino acids, their D/L enantiomers, protein digests, intact proteins, and protein complexes. With these capabilities, CE-MS is poised to become the multipurpose tool of separation scientists. More recently, an eight-capillary CE in conjunction with an 8-inlet mass spectrometry has allowed 8 CE-MS analyses to be performed concurrently, significantly increasing throughput.
authors: Museum Conservation Institute, Smithsonian Institution, Suitland, MD, USA. moinim@si.edu
authors: Mehdi
authors: M
authors: Moini
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D056831:Coordination Complexes / Q000737:chemistry / Q000302:isolation & purification; D043844:Crown Ethers; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods / Q000592:standards; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008667:Metalloproteins / Q000737:chemistry / Q000302:isolation & purification; D046912:Multiprotein Complexes / Q000302:isolation & purification*; D016062:Porosity; D012015:Reference Standards; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods / Q000592:standards; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D056831:Coordination Complexes; D043844:Crown Ethers; D004573:Electrolytes; D008667:Metalloproteins; D046912:Multiprotein Complexes; C015762:18-crown-6