title: CYCLIN-DEPENDENT KINASE G1 is associated with the spliceosome to regulate CALLOSE SYNTHASE5 splicing and pollen wall formation in Arabidopsis.
abstract: Arabidopsis thaliana CYCLIN-DEPEDENT KINASE G1 (CDKG1) belongs to the family of cyclin-dependent protein kinases that were originally characterized as cell cycle regulators in eukaryotes. Here, we report that CDKG1 regulates pre-mRNA splicing of CALLOSE SYNTHASE5 (CalS5) and, therefore, pollen wall formation. The knockout mutant cdkg1 exhibits reduced male fertility with impaired callose synthesis and abnormal pollen wall formation. The sixth intron in CalS5 pre-mRNA, a rare type of intron with a GC 5' splice site, is abnormally spliced in cdkg1. RNA immunoprecipitation analysis suggests that CDKG1 is associated with this intron. CDKG1 contains N-terminal Ser/Arg (RS) motifs and interacts with splicing factor Arginine/Serine-Rich Zinc Knuckle-Containing Protein33 (RSZ33) through its RS region to regulate proper splicing. CDKG1 and RS-containing Zinc Finger Protein22 (SRZ22), a splicing factor interacting with RSZ33 and U1 small nuclear ribonucleoprotein particle (snRNP) component U1-70k, colocalize in nuclear speckles and reside in the same complex. We propose that CDKG1 is recruited to U1 snRNP through RSZ33 to facilitate the splicing of the sixth intron of CalS5.
authors: College of Tourism, Shanghai Normal University, Shanghai 200234, China.
authors: Xue-Yong
authors: XY
authors: Huang
authors: Jin
authors: J
authors: Niu
authors: Ming-Xi
authors: MX
authors: Sun
authors: Jun
authors: J
authors: Zhu
authors: Ju-Fang
authors: JF
authors: Gao
authors: Jun
authors: J
authors: Yang
authors: Que
authors: Q
authors: Zhou
authors: Zhong-Nan
authors: ZN
authors: Yang
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D005936:Glucans / Q000235:genetics / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D007438:Introns; D009154:Mutation; D051479:Plant Infertility / Q000235:genetics; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics / Q000378:metabolism*; D012322:RNA Precursors; D012326:RNA Splicing; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics / Q000378:metabolism; D017381:Spliceosomes / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C469364:At2g37340 protein, Arabidopsis; D005936:Glucans; D012322:RNA Precursors; D017411:Ribonucleoproteins, Small Nuclear; C048306:callose; C585562:CalS5 protein, Arabidopsis; D005964:Glucosyltransferases; D018844:Cyclin-Dependent Kinases
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