title: Antifungal activity of nanocapsule suspensions containing tea tree oil on the growth of Trichophyton rubrum.
abstract: The aim of this study was to evaluate, for the first time, the antifungal efficacy of nanocapsules and nanoemulsions containing Melaleuca alternifolia essential oil (tea tree oil) in an onychomycosis model. The antifungal activity of nanostructured formulations was evaluated against Trichophyton rubrum in two different in vitro models of dermatophyte nail infection. First, nail powder was infected with T. rubrum in a 96-well plate and then treated with the formulations. After 7 and 14 days, cell viability was verified. The plate counts for the samples were 2.37, 1.45 and 1.0 log CFU mL(-1) (emulsion, nanoemulsion containing tea tree oil and nanocapsules containing tea tree oil, respectively). A second model employed nails fragments which were infected with the microorganism and treated with the formulations. The diameter of fungal colony was measured. The areas obtained were 2.88 ± 2.08 mm(2), 14.59 ± 2.01 mm(2), 40.98 ± 2.76 mm(2) and 38.72 ± 1.22 mm(2) for the nanocapsules containing tea tree oil, nanoemulsion containing tea tree oil, emulsion and untreated nail, respectively. Nail infection models demonstrated the ability of the formulations to reduce T. rubrum growth, with the inclusion of oil in nanocapsules being most efficient.
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors: F C
authors: FC
authors: Flores
authors: J A
authors: JA
authors: de Lima
authors: R F
authors: RF
authors: Ribeiro
authors: S H
authors: SH
authors: Alves
authors: C M B
authors: CM
authors: Rolim
authors: R C R
authors: RC
authors: Beck
authors: Cristiane Bona
authors: CB
authors: da Silva
mesh_terms: D015169:Colony Count, Microbial; D004337:Drug Carriers; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D050296:Microbial Viability / Q000187:drug effects; D009262:Nails / Q000382:microbiology; D053769:Nanocapsules; D013535:Suspensions; D020947:Tea Tree Oil / Q000494:pharmacology*; D014249:Trichophyton / Q000187:drug effects*
chemical_list: D004337:Drug Carriers; D053769:Nanocapsules; D013535:Suspensions; D020947:Tea Tree Oil
references: Med Mycol. 2009 Nov;47(7):753-8
references: 19888808
references: Biomacromolecules. 2010 Oct 11;11(10):2810-7
references: 20845938
references: J Pharm Pharm Sci. 2011;14(1):100-16
references: 21501557
references: Arch Intern Med. 2005 Aug 8-22;165(15):1756-61
references: 16087824
references: J Infect Chemother. 2002 Dec;8(4):331-5
references: 12525893
references: Trends Microbiol. 2009 Mar;17(3):100-8
references: 19243949
references: Mycopathologia. 2008 Nov-Dec;166(5-6):257-65
references: 18478364
references: Int J Pharm. 2004 Aug 6;280(1-2):241-51
references: 15265563
references: Eur J Pharm Sci. 2004 Mar;21(4):471-7
references: 14998577
references: Eur J Pharm Sci. 2007 Dec;32(4-5):254-60
references: 17928205
references: Food Chem Toxicol. 2008 Feb;46(2):446-75
references: 17996351
references: Clin Microbiol Rev. 2006 Jan;19(1):50-62
references: 16418522
references: Int J Pharm. 2002 Apr 2;236(1-2):1-26
references: 11891066
references: Mycopathologia. 1999 Nov;148(2):79-82
references: 11189747
references: J Eur Acad Dermatol Venereol. 2009 Jul;23(7):773-81
references: 19453778
references: Sci Technol Adv Mater. 2008 Sep 1;9(3):035004
references: 27878001
references: Nanomedicine. 2010 Feb;6(1):9-24
references: 19447208
references: Int J Pharm. 2007 Apr 4;334(1-2):166-72
references: 17113253
references: Food Chem Toxicol. 2006 May;44(5):616-25
references: 16243420
references: Inflamm Res. 2000 Nov;49(11):619-26
references: 11131302
references: J Control Release. 2002 Aug 21;82(2-3):319-34
references: 12175746
references: Drug Target Insights. 2007;2:147-57
references: 21901071