title: Core oligosaccharide of Plesiomonas shigelloides PCM 2231 (Serotype O17) lipopolysaccharide--structural and serological analysis.
abstract: The herein presented complete structure of the core oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 2231 (serotype O17) was investigated by (1)H, (13)C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The core oligosaccharide is composed of an undecasaccharide, which represents the second core type identified for P. shigelloides serotype O17 LPS. This structure is similar to that of the core oligosaccharide of P. shigelloides strains 302-73 (serotype O1) and 7-63 (serotype O17) and differs from these only by one sugar residue. Serological screening of 55 strains of P. shigelloides with the use of serum against identified core oligosaccharide conjugated with bovine serum albumin (BSA) indicated the presence of similar structures in the LPS core region of 28 O-serotypes. This observation suggests that the core oligosaccharide structure present in strain PCM 2231 could be the most common type among P. shigelloides lipopolysaccharides.
authors: Department of Immunochemistry, Ludwik Hirszfeld Institute Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland. aniaaugustyniuk@iitd.pan.wroc.pl
authors: Anna
authors: A
authors: Maciejewska
authors: Jolanta
authors: J
authors: Lukasiewicz
authors: Marta
authors: M
authors: Kaszowska
authors: Aleksandra
authors: A
authors: Man-Kupisinska
authors: Wojciech
authors: W
authors: Jachymek
authors: Czeslaw
authors: C
authors: Lugowski
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D016981:Plesiomonas / Q000145:classification* / Q000378:metabolism*; D011817:Rabbits
chemical_list: D008070:Lipopolysaccharides; D009844:Oligosaccharides
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