title: High throughput genomic screen identifies multiple factors that promote cooperative Wnt signaling.
abstract: Previous studies have demonstrated that certain Wnt ligands can promote high levels of cooperative signaling in a cell type specific manner. To explore the underlying mechanism of this cooperative Wnt signaling, we performed a high-throughput screen of more than 14,000 cDNAs to identify genes that promote cooperative Wnt signaling in the context of a single Wnt ligand, Wnt2. This screen identified several homeobox factors including Msx2, Nkx5.2, and Esx1, in addition to other factors known to promote Wnt signaling including Pias4. Generation of dominant-active or dominant-negative forms of Msx2 indicate that the mechanism by which homeobox factors cooperatively promote Wnt signaling is through their ability to repress gene transcription. These data identify a broad homeobox code, which acts to increase Wnt signaling through transcriptional repression.
authors: Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
authors: Mayumi F
authors: MF
authors: Miller
authors: Ethan David
authors: ED
authors: Cohen
authors: Julie E
authors: JE
authors: Baggs
authors: John B
authors: JB
authors: Hogenesch
authors: Edward E
authors: EE
authors: Morrisey
mesh_terms: D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D023281:Genomics / Q000379:methods; D057166:High-Throughput Screening Assays; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D051153:Wnt Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway
chemical_list: D018398:Homeodomain Proteins; D008024:Ligands; D014157:Transcription Factors; D051153:Wnt Proteins
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