title: Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells.
abstract: Recent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized equine amniotic membrane-derived mesenchymal stem cells (eAM-MSCs) that were cultured and maintained in low glucose Dulbecco's modified Eagle's medium. The proliferation of eAM-MSCs was measured based on the cumulative population doubling level (CPDL). Immunophenotyping of eAM-MSCs by flow cytometry showed that the major population was of mesenchymal origin. To confirm differentiation potential, a multi-lineage differentiation assay was conducted. We found that under appropriate conditions, eAM-MSCs are capable of multi-lineage differentiation. Our results indicated that eAM-MSCs may be a good source of stem cells, making them potentially useful for veterinary regenerative medicine and cell-based therapy.
authors: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors: Min-Soo
authors: MS
authors: Seo
authors: Sang-Bum
authors: SB
authors: Park
authors: Hyung-Sik
authors: HS
authors: Kim
authors: Jun-gu
authors: JG
authors: Kang
authors: Joon-Seok
authors: JS
authors: Chae
authors: Kyung-Sun
authors: KS
authors: Kang
mesh_terms: D050156:Adipogenesis; D000650:Amnion / Q000166:cytology* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D020219:Chondrogenesis; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006736:Horses; D016130:Immunophenotyping / Q000662:veterinary; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis
keywords: amnion; amniotic membrane; equine; isolation; mesenchymal stem cells
references: Vet Immunol Immunopathol. 2009 Dec 15;132(2-4):303-6
references: 19647331
references: BMC Biotechnol. 2007 May 30;7:26
references: 17537254
references: J Orthop Res. 2009 Dec;27(12):1675-80
references: 19544397
references: J Orthop Res. 2007 Jul;25(7):913-25
references: 17405160
references: Cell Biol Int. 2006 Sep;30(9):681-7
references: 16870478
references: PLoS One. 2012;7(9):e44693
references: 23024756
references: Cytotherapy. 2006;8(4):315-7
references: 16923606
references: Cell Transplant. 2009;18(2):111-8
references: 19499700
references: J Burn Care Res. 2008 Nov-Dec;29(6):907-16
references: 18849850
references: Histochem Cell Biol. 2009 Feb;131(2):267-82
references: 18836737
references: Nat Med. 2008 Feb;14(2):181-7
references: 18193058
references: Stem Cells Dev. 2010 Oct;19(10):1449-70
references: 20486777
references: Gene. 2009 Mar 15;433(1-2):1-7
references: 19135507
references: Rom J Morphol Embryol. 2009;50(1):73-7
references: 19221648
references: Am J Vet Res. 2008 Jul;69(7):928-37
references: 18593247
references: Curr Protoc Stem Cell Biol. 2010 Mar;Chapter 1:Unit 1E.5
references: 20200854
references: Am J Vet Res. 2006 Nov;67(11):1921-8
references: 17078756
references: Biomaterials. 2008 Jul;29(19):2923-31
references: 18417207
references: Stem Cells. 2008 Feb;26(2):300-11
references: 17975221
references: Cytotherapy. 2011 Mar;13(3):341-9
references: 20860427
references: Blood. 2004 Mar 1;103(5):1669-75
references: 14576065
references: Am J Vet Res. 2007 Oct;68(10):1095-105
references: 17916017
references: Cell Transplant. 2010;19(3):279-89
references: 19995482
references: Stem Cells Dev. 2011 Mar;20(3):547-57
references: 20923277
references: Methods Mol Biol. 2011;698:75-88
references: 21431512
references: Theriogenology. 2011 May;75(8):1431-43
references: 21196039
references: Tissue Eng Part C Methods. 2011 Jan;17(1):49-59
references: 20673138
references: J Orthop Res. 2009 Oct;27(10):1392-8
references: 19350658