title: Early steps of double-strand break repair in Bacillus subtilis.
abstract: All organisms rely on integrated networks to repair DNA double-strand breaks (DSBs) in order to preserve the integrity of the genetic information, to re-establish replication, and to ensure proper chromosomal segregation. Genetic, cytological, biochemical and structural approaches have been used to analyze how Bacillus subtilis senses DNA damage and responds to DSBs. RecN, which is among the first responders to DNA DSBs, promotes the ordered recruitment of repair proteins to the site of a lesion. Cells have evolved different mechanisms for efficient end processing to create a 3'-tailed duplex DNA, the substrate for RecA binding, in the repair of one- and two-ended DSBs. Strand continuity is re-established via homologous recombination (HR), utilizing an intact homologous DNA molecule as a template. In the absence of transient diploidy or of HR, however, two-ended DSBs can be directly re-ligated via error-prone non-homologous end-joining. Here we review recent findings that shed light on the early stages of DSB repair in Firmicutes.
authors: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Darwin 3, Cantoblanco, 28049 Madrid, Spain.
authors: Juan C
authors: JC
authors: Alonso
authors: Paula P
authors: PP
authors: Cardenas
authors: Humberto
authors: H
authors: Sanchez
authors: James
authors: J
authors: Hejna
authors: Yuki
authors: Y
authors: Suzuki
authors: Kunio
authors: K
authors: Takeyasu
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000502:physiology; D002876:Chromosomes, Bacterial / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair; D004260:DNA Repair; D004262:DNA Restriction Enzymes / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D059767:Recombinational DNA Repair
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C047879:RecN protein, Bacteria