title: Effect of gum arabic on oxidative stress and inflammation in adenine-induced chronic renal failure in rats.
abstract: Inflammation and oxidative stress are known to be involved in the pathogenesis of chronic kidney disease in humans, and in chronic renal failure (CRF) in rats. The aim of this work was to study the role of inflammation and oxidative stress in adenine-induced CRF and the effect thereon of the purported nephroprotective agent gum arabic (GA). Rats were divided into four groups and treated for 4 weeks as follows: control, adenine in feed (0.75%, w/w), GA in drinking water (15%, w/v) and adenine+GA, as before. Urine, blood and kidneys were collected from the rats at the end of the treatment for analysis of conventional renal function tests (plasma creatinine and urea concentration). In addition, the concentrations of the pro-inflammatory cytokine TNF-α and the oxidative stress markers glutathione and superoxide dismutase, renal apoptosis, superoxide formation and DNA double strand break frequency, detected by immunohistochemistry for γ-H2AX, were measured. Adenine significantly increased the concentrations of urea and creatinine in plasma, significantly decreased the creatinine clearance and induced significant increases in the concentration of the measured inflammatory mediators. Further, it caused oxidative stress and DNA damage. Treatment with GA significantly ameliorated these actions. The mechanism of the reported salutary effect of GA in adenine-induced CRF is associated with mitigation of the adenine-induced inflammation and generation of free radicals.
authors: Department of Pharmacology and Clinical Pharmacy, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khod, Muscat, Sultanate of Oman.
authors: Badreldin H
authors: BH
authors: Ali
authors: Isehaq
authors: I
authors: Al-Husseni
authors: Sumyia
authors: S
authors: Beegam
authors: Ahmed
authors: A
authors: Al-Shukaili
authors: Abderrahim
authors: A
authors: Nemmar
authors: Simone
authors: S
authors: Schierling
authors: Nina
authors: N
authors: Queisser
authors: Nicole
authors: N
authors: Schupp
mesh_terms: D000225:Adenine / Q000009:adverse effects*; D000818:Animals; D003404:Creatinine / Q000097:blood; D006170:Gum Arabic / Q000494:pharmacology* / Q000627:therapeutic use; D006657:Histones / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology; D016753:Interleukin-10 / Q000097:blood; D007676:Kidney Failure, Chronic / Q000139:chemically induced* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D014508:Urea / Q000097:blood
chemical_list: D006657:Histones; D010750:Phosphoproteins; C577575:gamma-H2AX protein, rat; D016753:Interleukin-10; D014508:Urea; D006170:Gum Arabic; D003404:Creatinine; D000225:Adenine
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