title: Effect of lentinan combined with docetaxel and cisplatin on the proliferation and apoptosis of BGC823 cells.
abstract: We studied the inhibitory effects of lentinan alone or lentinan combined with docetaxel and cisplatin on growth of gastric cancer cell line BGC823. The cells were divided into lentinan group, docetaxel combined with cisplatin group, and lentinan combined with docetaxel and cisplatin group. Gastric cancer cell line BGC823 was treated with different concentrations of drugs in each group. Tetrazolium-based colorimetric assay (MTT), Annexin V/propidium iodide method and flow cytometry were used to determine the proliferation and apoptosis of the cells in each group. The inhibition ratio was positively related with the concentrations of drugs when BGC823 cells were treated with docetaxel combined with cisplatin from low to high dose. The inhibition ratio of each group further increased after lentinan was added into the medium. The apoptosis rate of 6.25 μg/ml lentinan on BGC823 cells was 19.84 %. The apoptosis rate of BGC823 cells was significantly increased from 50.22 % to 72.06 % after treatment with 6.25 μg/ml lentinan combined with 2.5 μg/ml docetaxel and 50 μg/ml cisplatin. Lentinan has an inhibitory effect on the proliferation of gastric cancer cell line BGC823. Lentinan combined with docetaxel and cisplatin increases the inhibitory effect on the proliferation of BGC823 cells mediated by docetaxel combined with cisplatin. Low concentration of lentinan combined with docetaxel and cisplatin has better therapeutic effects on the proliferation of BGC823 cells compared with high concentrations of docetaxel combined with cisplatin. Lentinan has the ability of inducing BGC823 cell apoptosis and this effect is enhanced when combined with docetaxel and cisplatin.
authors: Department of Oncology, Shengjing Hospital of China Medical University, 39 Hua'xiang Road, TieXi District, Shenyang, 110022, Liaoning Province, China. cmu2hzhaoli@yahoo.cn
authors: Li
authors: L
authors: Zhao
authors: Yuping
authors: Y
authors: Xiao
authors: Nan
authors: N
authors: Xiao
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000008:administration & dosage; D000077143:Docetaxel; D005434:Flow Cytometry; D006801:Humans; D007912:Lentinan / Q000008:administration & dosage; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D043823:Taxoids / Q000008:administration & dosage; D014407:Tumor Cells, Cultured
chemical_list: D043823:Taxoids; D000077143:Docetaxel; D007912:Lentinan; D002945:Cisplatin
references: Anticancer Drugs. 1997 Jul;8(6):632-6
references: 9300579
references: Carbohydr Res. 2005 Jun 13;340(8):1515-21
references: 15882854
references: Eur Rev Med Pharmacol Sci. 2010 Apr;14(4):302-8
references: 20496539
references: J Clin Oncol. 2006 Jun 20;24(18):2903-9
references: 16782930
references: J Altern Complement Med. 2002 Oct;8(5):581-9
references: 12470439
references: Mol Med Rep. 2012 Mar;5(3):745-8
references: 22200763
references: Anticancer Res. 2009 Jul;29(7):2739-45
references: 19596954
references: Nature. 1969 May 17;222(5194):687-8
references: 5768289
references: Cancer Res. 1984 Nov;44(11):5132-7
references: 6488173
references: Jpn J Cancer Res. 1996 Nov;87(11):1171-8
references: 9045947
references: Hepatogastroenterology. 2009 Mar-Apr;56(90):547-50
references: 19579640
references: Int J Oncol. 2010 Sep;37(3):623-31
references: 20664931
references: Anticancer Res. 2006 Nov-Dec;26(6A):4015-8
references: 17195451
references: World J Clin Oncol. 2011 Oct 10;2(10):339-43
references: 21994907
references: Int Immunopharmacol. 2006 Apr;6(4):686-96
references: 16504933
references: Asian Pac J Cancer Prev. 2012;13(8):3803-7
references: 23098474
references: Gan To Kagaku Ryoho. 2005 Aug;32(8):1209-15
references: 16121931
references: Can J Oncol. 1996 Jun;6(1):443-57
references: 8853517
references: Jpn J Clin Oncol. 2002 Jul;32(7):248-54
references: 12324575
references: Gan To Kagaku Ryoho. 2011 Feb;38(2):293-5
references: 21368498
references: Poult Sci. 2003 May;82(5):760-6
references: 12762398