title: Post-polyketide synthase steps in iso-migrastatin biosynthesis, featuring tailoring enzymes with broad substrate specificity.
abstract: The iso-migrastatin (iso-MGS) biosynthetic gene cluster from Streptomyces platensis NRRL 18993 consists of 11 genes, featuring an acyltransferase (AT)-less type I polyketide synthase (PKS) and three tailoring enzymes MgsIJK. Systematic inactivation of mgsIJK in S. platensis enabled us to (i) identify two nascent products of the iso-MGS AT-less type I PKS, establishing an unprecedented novel feature for AT-less type I PKSs, and (ii) account for the formation of all known post-PKS biosynthetic intermediates generated by the three tailoring enzymes MgsIJK, which possessed significant substrate promiscuities.
authors: Department of Chemistry, The Scripps Research Institute, Jupiter, Florida 33458, USA.
authors: Ming
authors: M
authors: Ma
authors: Thomas
authors: T
authors: Kwong
authors: Si-Kyu
authors: SK
authors: Lim
authors: Jianhua
authors: J
authors: Ju
authors: Jeremy R
authors: JR
authors: Lohman
authors: Ben
authors: B
authors: Shen
mesh_terms: D053898:Biosynthetic Pathways; D018942:Macrolides / Q000737:chemistry; D015394:Molecular Structure; D010881:Piperidones / Q000737:chemistry; D048630:Polyketide Synthases / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D018942:Macrolides; D010881:Piperidones; C455261:isomigrastatin; D048630:Polyketide Synthases
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