title: Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens.
abstract: The rumen anaerobic cellulolytic bacterium Eubacterium cellulosolvens produces a large range of cellulases and hemicellulases responsible for the efficient hydrolysis of plant cell wall polysaccharides. One of these enzymes, endoglucanase Cel5A, comprises a tandemly repeated carbohydrate-binding module (CBM65) fused to a glycoside hydrolase family 5 (Cel5A) catalytic domain, joined by flexible linker sequences. The second carbohydrate-binding module located at the C-terminus side of the endoglucanase (CBM65B) has been co-crystallized with either cellohexaose or xyloglucan heptasaccharide. The crystals belong to the hexagonal space group P6(5) and tetragonal space group P4(3)2(1)2, containing a single molecule in the asymmetric unit. The structures of CBM65B have been solved by molecular replacement.
authors: CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
authors: Immacolata
authors: I
authors: Venditto
authors: Arnaud
authors: A
authors: Baslé
authors: Ana S
authors: AS
authors: Luís
authors: Max J
authors: MJ
authors: Temple
authors: Luís M A
authors: LM
authors: Ferreira
authors: Carlos M G A
authors: CM
authors: Fontes
authors: Harry J
authors: HJ
authors: Gilbert
authors: Shabir
authors: S
authors: Najmudin
mesh_terms: D000595:Amino Acid Sequence; D002480:Cellulase / Q000737:chemistry* / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D005051:Eubacterium / Q000201:enzymology*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000302:isolation & purification*; D016415:Sequence Alignment
chemical_list: D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; C407024:endoglucanase Cel5A; D002480:Cellulase
keywords: Eubacterium cellulosolvens; carbohydrate-binding module (CBM65)
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