title: Increased plasma angiogenin level is associated and may contribute to decreased T-cell zeta-chain expression in hemodialysis patients.
abstract: Hemodialysis (HD) patients are characterized by impaired T-cell function at least in part because of T-cell zeta-chain downregulation due to inflammation. Angiogenin responds as an acute phase reactant, is increased in HD patients, suppresses T-cell function and increased angiogenin level is co-localized with T-cell zeta-chain downregulation in various pathologies. Angiogenin can inhibit translation of proteins, which lack internal ribosomal entry sites in the corresponding mRNAs. In this study, the possible effect of angiogenin on T-cell zeta-chain downregulation was evaluated. Thirty HD patients and 21 healthy volunteers participated. T-cell zeta-chain expression was assessed with flow cytometry, plasma angiogenin and serum IL-6 with ELISA and serum C-reactive protein with an immunoturbidimetric method. Two available software tools were used for predicting the presence or not of internal ribosomal entry sites in T-cell zeta-chain mRNA sequence. In silico analysis of T-cell zeta-chain mRNA sequence failed to reveal the presence of internal ribosomal entry sites. T-cell zeta-chain expression was lower in HD patients than in healthy volunteers (1.86 ± 0.63 vs. 4.73 ± 3.22). In HD patients, C-reactive protein as well as IL-6 were higher than in healthy volunteers (10.04 ± 15.13 mg/L vs. 3.43 ± 0.98 mg/L and 15.06 ± 13.08 pg/mL vs. 2.11 ± 2.10 pg/mL respectively). Angiogenin was higher in HD patients than in healthy volunteers (483.20 ± 154.07 ng/mL vs. 259.98 ± 64.15 ng/mL). Neither C-reactive protein, nor IL-6 was associated with angiogenin or T-cell zeta-chain expression. Angiogenin concentration was negatively related to the expression of T-cell zeta-chain (r = -0.410, P = 0.025). Increased angiogenin may contribute to decreased T-cell zeta-chain expression in HD patients.
authors: Nephrology Department, Medical School, University of Thessaly, Larissa, Greece. teleftheriadis@yahoo.com
authors: Theodoros
authors: T
authors: Eleftheriadis
authors: Charalambos
authors: C
authors: Kartsios
authors: Georgios
authors: G
authors: Pissas
authors: Vassilios
authors: V
authors: Liakopoulos
authors: Georgia
authors: G
authors: Antoniadi
authors: Grammati
authors: G
authors: Galaktidou
authors: Ioannis
authors: I
authors: Stefanidis
mesh_terms: D000368:Aged; D001483:Base Sequence; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D012333:RNA, Messenger / Q000378:metabolism; D006435:Renal Dialysis; D012259:Ribonuclease, Pancreatic / Q000097:blood*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger; D002097:C-Reactive Protein; C046366:angiogenin; D012259:Ribonuclease, Pancreatic