title: [Rapid screening and confirmation of hormones in health foods by high performance liquid chromatography-ion trap-time of flight tandem mass spectrometry].
abstract: A method was developed for the screening, confirmation and quantification of 21 hormones including progesterones, etrogens, glucocorticoids and resorcylic acid lactones in health foods by high performance liquid chromatography-ion trap-time of flight tandem mass spectrometry (HPLC-IT-TOF-MS). The analytes in the sample were extracted with acetonitrile containing 1.0% (v/v) acetic acid and the extract was purified with the mixed QuEChERS sorbents. In the chromatographic analysis, the 21 target compounds were separated on a C18 column with the gradient elution using the mobile phases of acetonitrile and water containing 0.1% acetic acid. The mass analyzer was performed in positive and negative full scan modes in one injection at the same time. The results showed that the linear ranges of the 21 hormones were 5.0-250 microg/L with the correlation coefficients above 0.993 and the limits of quantification (LOQ, S/N > or = 10) were 2.0-5.0 microg/kg and 1.0-2.5 microg/L for capsule and oral solution, respectively. The method validation was carried out at three spiked levels, and the recoveries were 60.2%-116.0% with the relative standard deviations (RSDs) of 7.0%-18.3%. The screening of analytes was performed by precision mass matching and library searching. The secondary fragment ion spectra were employed to the confirmation. This method is simple, fast, reliable, and can be applied to the simultaneous screening and determination of hormones in health foods.
authors: Inspection and Quarantine Technology Center, Hunan Entry-Exit Inspection and Quarantine Bureau Changsha 410004, China.
authors: Meiling
authors: M
authors: Wang
authors: Hongfei
authors: H
authors: Yan
authors: Shanliang
authors: S
authors: Fu
authors: Fan
authors: F
authors: Zhang
authors: Jinting
authors: J
authors: Yao
authors: Hua
authors: H
authors: Dai
authors: Yongjun
authors: Y
authors: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004967:Estrogens / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D055951:Functional Food / Q000032:analysis*; D005938:Glucocorticoids / Q000032:analysis; D006728:Hormones / Q000032:analysis*; D011374:Progesterone / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004967:Estrogens; D005938:Glucocorticoids; D006728:Hormones; D011374:Progesterone