title: FIP200 regulates targeting of Atg16L1 to the isolation membrane.
abstract: Autophagosome formation is a dynamic process that is strictly controlled by autophagy-related (Atg) proteins. However, how these Atg proteins are recruited to the autophagosome formation site or autophagic membranes remains poorly understood. Here, we found that FIP200, which is involved in proximal events, directly interacts with Atg16L1, one of the downstream Atg factors, in an Atg14- and phosphatidylinositol 3-kinase-independent manner. Atg16L1 deletion mutants, which lack the FIP200-interacting domain, are defective in proper membrane targeting. Thus, FIP200 regulates not only early events but also late events of autophagosome formation through direct interaction with Atg16L1.
authors: Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.
authors: Taki
authors: T
authors: Nishimura
authors: Takeshi
authors: T
authors: Kaizuka
authors: Ken
authors: K
authors: Cadwell
authors: Mayurbhai H
authors: MH
authors: Sahani
authors: Tatsuya
authors: T
authors: Saitoh
authors: Shizuo
authors: S
authors: Akira
authors: Herbert W
authors: HW
authors: Virgin
authors: Noboru
authors: N
authors: Mizushima
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D017353:Gene Deletion; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D033921:Vesicular Transport Proteins / Q000378:metabolism
chemical_list: C561107:Atg14 protein, mouse; C532820:Atg16l1 protein, mouse; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C428743:Rb1cc1 protein, mouse; D033921:Vesicular Transport Proteins; D058539:Phosphatidylinositol 3-Kinase
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