title: Neuroprotective effect of a cell-free extract derived from human adipose stem cells in experimental stroke models.
abstract: A recent study suggested that a cell-free extract of human adipose stem cells (hASCs-E) has beneficial effects on neurological diseases by modulating the host environment. Here, we investigated the effects of hASCs-E in several experimental models of stroke in vitro (oxygen and glucose deprivation, OGD) and in vivo (transient or permanent focal cerebral ischemia and intracerebral hemorrhage, ICH). Ischemia was induced in vitro in Neuro2A cells, and the hASCs-E was applied 24h before the OGD or concurrently. Focal cerebral ischemia was induced by unilateral intraluminal thread occlusion of the middle cerebral artery (MCA) in rats for 90min or permanently, or by unilateral MCA microsurgical direct electrocoagulation in mice. The ICH model was induced with an intracerebral injection of collagenase in rats. The hASCs-E was intraperitoneally administered 1h after the stroke insults. Treatment of the hASCs-E led to a substantially high viability in the lactate dehydrogenase and WST-1 assays in the in vitro ischemic model. The cerebral ischemic and ICH model treated with hASCs-E showed decreased ischemic volume and reduced brain water content and hemorrhage volume. The ICH model treated with hASCs-E exhibited better performance on the modified limb placing test. The expression of many genes related to inflammation, immune response, and cell-death was changed substantially in the ischemic rats or neuronal cells treated with the hASCs-E. These results reveal a neuroprotective role of hASCs-E in animal models of stroke, and suggest the feasible application of stem cell-based, noninvasive therapy for treating stroke.
authors: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea. clark@kaist.ac.kr
authors: Daejong
authors: D
authors: Jeon
authors: Kon
authors: K
authors: Chu
authors: Soon-Tae
authors: ST
authors: Lee
authors: Keun-Hwa
authors: KH
authors: Jung
authors: Jae-Jun
authors: JJ
authors: Ban
authors: Dong-Kyu
authors: DK
authors: Park
authors: Hye-Jin
authors: HJ
authors: Yoon
authors: Seungmoon
authors: S
authors: Jung
authors: Hyunwoo
authors: H
authors: Yang
authors: Byung Sun
authors: BS
authors: Kim
authors: Ji Ye
authors: JY
authors: Choi
authors: So Hee
authors: SH
authors: Kim
authors: Jeong-Min
authors: JM
authors: Kim
authors: Chong-Hyun
authors: CH
authors: Won
authors: Manho
authors: M
authors: Kim
authors: Sang Kun
authors: SK
authors: Lee
authors: Jae-Kyu
authors: JK
authors: Roh
mesh_terms: D017667:Adipocytes / Q000737:chemistry*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002474:Cell-Free System; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000737:chemistry*; D020521:Stroke / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome / Q000187:drug effects
chemical_list: D018696:Neuroprotective Agents