title: Multi-electrode array technologies for neuroscience and cardiology.
abstract: At present, the prime methodology for studying neuronal circuit-connectivity, physiology and pathology under in vitro or in vivo conditions is by using substrate-integrated microelectrode arrays. Although this methodology permits simultaneous, cell-non-invasive, long-term recordings of extracellular field potentials generated by action potentials, it is 'blind' to subthreshold synaptic potentials generated by single cells. On the other hand, intracellular recordings of the full electrophysiological repertoire (subthreshold synaptic potentials, membrane oscillations and action potentials) are, at present, obtained only by sharp or patch microelectrodes. These, however, are limited to single cells at a time and for short durations. Recently a number of laboratories began to merge the advantages of extracellular microelectrode arrays and intracellular microelectrodes. This Review describes the novel approaches, identifying their strengths and limitations from the point of view of the end users--with the intention to help steer the bioengineering efforts towards the needs of brain-circuit research.
authors: The Alexander Silberman Life Sciences Institute, and the Harvey M. Kruger Family Center for Nanoscience, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. spira@cc.huji.ac.il
authors: Micha E
authors: ME
authors: Spira
authors: Aviad
authors: A
authors: Hai
mesh_terms: D000200:Action Potentials / Q000502:physiology; D057005:Bioengineering; D002309:Cardiology / Q000379:methods*; D055724:Electrophysiological Phenomena; D006801:Humans; D046228:Microarray Analysis; D008839:Microelectrodes; D050997:Nanomedicine / Q000379:methods; D009474:Neurons / Q000502:physiology; D009488:Neurosciences / Q000379:methods*; D018408:Patch-Clamp Techniques
references: J Neurosci Methods. 1996 Nov;69(2):175-88
references: 8946321
references: Biosens Bioelectron. 1990;5(3):223-34
references: 2206490
references: Nanoscale. 2011 Feb;3(2):391-400
references: 20931126
references: Nat Nanotechnol. 2011 Dec 18;7(3):174-9
references: 22179566
references: Neuron. 1999 Dec;24(4):791-802
references: 10624943
references: Nat Rev Neurosci. 2012 May 18;13(6):407-20
references: 22595786
references: Pure Appl Chem. 2011 Jan 1;83(12):2153-2169
references: 22707797
references: Prog Biophys Biophys Chem. 1957;7:1-85
references: 13485186
references: Nanotechnology. 2010 Feb 26;21(8):85303
references: 20101076
references: Biophys J. 2006 Apr 1;90(7):2600-11
references: 16399830
references: J Neurophysiol. 2006 Sep;96(3):1638-45
references: 16687618
references: Front Neuroeng. 2012 Aug 24;5:21
references: 22936913
references: Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:6473-6
references: 21096721
references: J Neurosci Methods. 2000 Aug 15;101(1):31-42
references: 10967359
references: J R Soc Interface. 2009 Dec 6;6(41):1153-65
references: 19474080
references: Annu Rev Physiol. 1984;46:455-72
references: 6143532
references: J Neurosci Methods. 1980 Feb;2(1):19-31
references: 7329089
references: J Neurosci Methods. 1999 Oct 30;93(1):61-7
references: 10598865
references: Lab Chip. 2006 Dec;6(12):1510-5
references: 17203154
references: Pflugers Arch. 2006 Dec;453(3):233-47
references: 17072639
references: Annu Rev Biochem. 2009;78:857-902
references: 19317650
references: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2006 Aug;192(8):777-84
references: 16550391
references: Nature. 2006 Jul 13;442(7099):164-71
references: 16838014
references: Phys Rev Lett. 2006 Jun 9;96(22):228102
references: 16803347
references: Lab Chip. 2012 Apr 7;12(7):1274-80
references: 22337001
references: Nat Nanotechnol. 2012 Feb 12;7(3):185-90
references: 22327876
references: Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:2726-9
references: 21096209
references: Neuron. 1997 Oct;19(4):735-41
references: 9354320
references: Jpn J Physiol. 1994;44(5):433-73
references: 7534361
references: Nat Methods. 2011 Nov 27;9(1):90-5
references: 22120467
references: J Neurosci Methods. 1989 Nov;30(2):91-106
references: 2586157
references: Annu Rev Neurosci. 2004;27:487-507
references: 15217341
references: Biomed Microdevices. 2009 Apr;11(2):495-501
references: 19067173
references: Methods Cell Biol. 2007;83:89-111
references: 17613306
references: J R Soc Interface. 2008 Feb 6;5(19):213-22
references: 17609177
references: Biosens Bioelectron. 2009 Mar 15;24(7):2191-8
references: 19157842
references: Proc Natl Acad Sci U S A. 2009 May 5;106(18):7309-13
references: 19365078
references: Nat Nanotechnol. 2012 Jan 10;7(3):180-4
references: 22231664
references: J Neural Eng. 2009 Dec;6(6):066009
references: 19918108
references: Trends Cogn Sci. 2011 Dec;15(12):592-600
references: 22055387
references: Nanotechnology. 2011 Jul 1;22(26):265104
references: 21586820
references: Philos Trans R Soc Lond B Biol Sci. 2009 Sep 12;364(1529):2453-67
references: 19651647
references: Nat Methods. 2010 Mar;7(3):200-2
references: 20118930
references: Lab Chip. 2011 Mar 21;11(6):1054-8
references: 21286648
references: Nano Lett. 2012 Jun 13;12(6):3329-33
references: 22583370
references: J Neurosci. 2006 Aug 16;26(33):8465-76
references: 16914671
references: J Neural Eng. 2011 Jun;8(3):034002
references: 21540486
references: Exp Cell Res. 1972 Sep;74(1):61-6
references: 4672477
references: Biophys J. 1985 Jan;47(1):71-7
references: 3978192
references: Annu Rev Immunol. 1999;17:593-623
references: 10358769
references: Neuron. 2008 Sep 25;59(6):902-13
references: 18817730
references: J Neurophysiol. 2010 Jul;104(1):559-68
references: 20427620
references: J Cell Sci. 2001 Mar;114(Pt 6):1061-77
references: 11228151
references: Lab Chip. 2012 Nov 7;12(21):4397-402
references: 22930315
references: Front Neuroeng. 2010 May 10;3:4
references: 20485465
references: Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5815-20
references: 20212151
references: J Neurophysiol. 2005 May;93(5):2987-3000
references: 15548620
references: Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7319-24
references: 12777621
references: Nature. 1986 Nov 27-Dec 3;324(6095):361-4
references: 3785405
references: Ann N Y Acad Sci. 2006 Dec;1093:143-60
references: 17312257
references: Eur Biophys J. 2001;30(1):17-26
references: 11372529
references: Annu Rev Biomed Eng. 2002;4:407-52
references: 12117764
references: Crit Rev Biomed Eng. 2011;39(1):45-61
references: 21488814
references: IEEE Trans Biomed Eng. 2003 Apr;50(4):501-9
references: 12723062
references: Nano Lett. 2011 May 11;11(5):2066-70
references: 21469728
references: Lab Chip. 2012 Aug 21;12(16):2865-73
references: 22678065
references: Nat Neurosci. 2004 May;7(5):456-61
references: 15114358
references: Cold Spring Harb Perspect Biol. 2012 Jun 01;4(6):
references: 22496389
references: Nat Nanotechnol. 2008 Jul;3(7):434-9
references: 18654569
references: J Neurosci Methods. 1982 Jan;5(1-2):13-22
references: 7057675
references: Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9112-7
references: 15967996
references: Science. 2010 Aug 13;329(5993):830-4
references: 20705858
references: Brain Res. 1987 Aug 25;418(2):255-61
references: 3676715
references: Anal Bioanal Chem. 2011 Mar;399(7):2313-29
references: 20676620
references: Front Neuroeng. 2011 Dec 08;4:14
references: 22163219
references: Nat Neurosci. 2010 Mar;13(3):353-60
references: 20118927
references: Science. 2002 Jun 28;296(5577):2395-8
references: 12089443
references: Annu Rev Cell Dev Biol. 2003;19:677-95
references: 14570586
references: J Neurophysiol. 2008 Jul;100(1):346-57
references: 18463183
references: Biosens Bioelectron. 2008 Jan 18;23(6):811-9
references: 17959368
references: Lab Chip. 2009 Sep 21;9(18):2644-51
references: 19704979
references: Neuron. 2005 May 19;46(4):529-32
references: 15944122