title: Use of Luminex xMAP bead-based suspension array for detecting microRNA in NSCLC tissues and its clinical application.
abstract: BACKGROUND
  We measured the expression of microRNA (miRNA) in non-small cell lung cancer (NSCLC) tissues using the Luminex xMAP bead-based suspension array. We discuss the feasibility of employing this method to detect miRNA in NSCLC and explore its value as a high-throughput miRNA array.
  
  
  METHODS
  We performed the methodological analysis of xMAP with oligoribonucleic acid references. We detected the expression of miR-21, miR, miR-31, miR-222, miR-145 and miR 40 NSCLC cancer tissues and adjacent normal tissues by xMAP bead-based suspension array. We selected miR-191 and miR-103 as the house-keeping genes (internal control). We also analyzed the relationship between xMAP and RT-PCR.
  
  
  RESULTS
  The methodological analysis parameters of xMAP are quite good. The expression of miR-21, miR, miR-31 and miR-222 was higher in NSCLC tissues than in adjacent tissues, while the expression of miR-145 and miR-126 was lower in NSCLC tissues than in adjacent tissues. The expression of miR-145 and miR-126 decreased with disease progression. The intraassay and interassay coefficients of variation were lower in xMAP than in RT-PCR. xMAP proved cheaper and more flexible in detecting multiple miRNAs of one sample.
  
  
  CONCLUSIONS
  The Luminex xMAP bead-based suspension array for detecting miRNA has many advantages, such as allowing a smaller sample size (only 2 μL), no sample amplification, fast detection, high throughput, and flexible combination of multiple detection targets. The high throughput testing technology shows a great advantage in saving time and labor. We found that the Luminex xMAP bead-based suspension array is a good and feasible method for detecting miRNA expression with high-throughput technology.
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Suzhou University, Laboratory of Clinical Immunology in Jiangsu Province, Suzhou, China. guguohao@yahoo.com.cn
authors: Yanan
authors: Y
authors: Wang
authors: Jinfang
authors: J
authors: Shi
authors: Yuanjian
authors: Y
authors: Wu
authors: Weidong
authors: W
authors: Xu
authors: Qinxi
authors: Q
authors: Wang
authors: Jing
authors: J
authors: Zhang
authors: Min
authors: M
authors: Jiang
authors: Guohao
authors: G
authors: Gu
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000556:secondary; D005240:Feasibility Studies; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D020043:Genes, Essential; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000032:analysis* / Q000302:isolation & purification; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D015345:Oligonucleotide Probes; C544749:MIRN103 microRNA, human; C527111:MIRN126 microRNA, human; C541010:MIRN145 microRNA, human; C546177:MIRN191 microRNA, human; C524940:MIRN21 microRNA, human; C525710:MIR222, human; C534612:MIRN31 microRNA, human