title: Elastic and conformational softness of a globular protein.
abstract: Flexibility, or softness, is crucial for protein function and consists of a conformational component, involving jumps between potential wells, and an elastic component, involving fluctuations within the wells. Combining molecular dynamics simulation with incoherent neutron scattering and light scattering measurements on green fluorescent protein, we reveal a relationship between the intrawell fluctuations and elastic moduli of the protein. This finding leads to a simple means of experimentally separating the conformational from the elastic atomic displacements.
authors: UT/ORNL Center for Molecular Biophysics, Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, Tennessee 37831-6309, USA.
authors: Liang
authors: L
authors: Hong
authors: Dennis C
authors: DC
authors: Glass
authors: Jonathan D
authors: JD
authors: Nickels
authors: Stefania
authors: S
authors: Perticaroli
authors: Zheng
authors: Z
authors: Yi
authors: Madhusudan
authors: M
authors: Tyagi
authors: Hugh
authors: H
authors: O'Neill
authors: Qiu
authors: Q
authors: Zhang
authors: Alexei P
authors: AP
authors: Sokolov
authors: Jeremy C
authors: JC
authors: Smith
mesh_terms: D004548:Elasticity; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008956:Models, Chemical; D056004:Molecular Dynamics Simulation; D033363:Neutron Diffraction; D011487:Protein Conformation
chemical_list: D049452:Green Fluorescent Proteins