title: Comparison of biochemical and molecular tests for detecting insecticide resistance due to insensitive acetylcholinesterase in Culex quinquefasciatus.
abstract: Insecticide resistance to organophosphates and carbamates can be the result of changes in acetylcholinesterase activity conferred by the ACE-1 mutation. Detection of this altered target site mutation is important in guiding informed decisions for resistance management. In this study we compared a competitive enzyme assay with a polymerase chain reaction assay utilizing a restriction enzyme. Both assays detected the ACE-1 mutation in Culex quinquefasciatus and agreement was 100%. The costs and benefits of each assay are presented.
authors: Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, 3150 Rampart Road, Fort Collins, CO 80521, USA.
authors: Mariah L
authors: ML
authors: Scott
authors: Janet C
authors: JC
authors: McAllister
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D003465:Culex / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D000110:Acetylcholinesterase
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