title: Simultaneous determination of opiates, methadone, amphetamines, cocaine, and metabolites in human placenta and umbilical cord by LC-MS/MS.
abstract: LC-MS/MS methods for the quantification of morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine, 6-acetylmorphine, cocaine, benzoylecgonine, ecgonine methyl ester, hydroxybenzoylecgonine, cocaethylene, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), methadone, and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in human placenta and umbilical cord were developed and validated. Specimens (1 ± 0.02 g) were homogenized with the Ultra-Turrax T8 disperser and centrifuged, and the supernatant was submitted to solid-phase extraction with Oasis MCX cartridges. Chromatographic separation was performed using an Atlantis T3 analytical column (100 × 2.1 mm, 3 μm) and a gradient of 0.1 % formic acid and acetonitrile. Selectivity was verified in 10 different blank specimens. The method was linear from 1-5 to 100-500 ng/g, depending on the analyte. Limits of detection and quantification ranged from 0.5 to 2.5 ng/g and 1 to 5 ng/g, respectively. Method imprecision was ≤15.3 %, except for MDMA at low quality control (18.1 %); accuracy, 87.1 to 114 %; extraction efficiency, 16.3 to 154.0 % (%CV = 1.8-39.4 %); matrix effect, -75.7 to 449.9 % (%CV = 3.5-50 %); and process efficiency, 8.7 to 316.0 %. The method was applied to authentic placenta and umbilical cord specimens from drug-user pregnant women.
authors: Toxicology Service, Institute of Forensic Sciences, University of Santiago de Compostela, Santiago de Compostela, Spain. ana.decastro@usc.es
authors: Ana
authors: A
authors: de Castro
authors: Ariana
authors: A
authors: Díaz
authors: Beatriz
authors: B
authors: Piñeiro
authors: Elena
authors: E
authors: Lendoiro
authors: Angelines
authors: A
authors: Cruz
authors: Manuel
authors: M
authors: López-Rivadulla
authors: Marta
authors: M
authors: Concheiro
mesh_terms: D000661:Amphetamine / Q000032:analysis* / Q000378:metabolism; D000701:Analgesics, Opioid / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D003042:Cocaine / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008691:Methadone / Q000032:analysis* / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D052616:Solid Phase Extraction / Q000379:methods; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014470:Umbilical Cord / Q000378:metabolism*
chemical_list: D000701:Analgesics, Opioid; D000661:Amphetamine; D003042:Cocaine; D008691:Methadone