title: [Protective effect of qingxin kaiqiao fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35].
abstract: OBJECTIVE
  To observe the protective effect of Qingxin Kaiqiao Fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35, in order to provide basis for clinical application of the formula.
  
  
  METHODS
  Sprague Dawley rats were orally administration with Qingxin Kaiqiao Fang (7.9 g x kg(-1)) twice a day for 3.5 days to prepare Qingxin Kaiqiao Fang containing cerebrospinal fluid. The nerve cell injury model was established by PC12 cells and Abeta25-35 with the concentration of 10 micromol x L(-1). The expressions of Bax, Bcl-2 and Caspase-3 mRNA were detected by immunohistochemical method in the PC12 cells.
  
  
  RESULTS
  The Qingxin Kaiqiao Fang group showed a significant higher PC12 cell activity than the model group, with decrease in Bax mRNA and Caspase-3 mRNA expressions and increase in Bcl-2 mRNA expression. There was a significant difference from the model group (P < 0.05, P < 0.01).
  
  
  CONCLUSIONS
  Qinxin Kaiqiao Fang shows a significant protective effect on Abeta25-35-induced nerve cell injury.
authors: Foundation College of Wenzhou Medical College, Wenzhou 325035, China.
authors: Guang-Hua
authors: GH
authors: Ye
authors: Hai-Yan
authors: HY
authors: Hu
authors: Xiang
authors: X
authors: Chen
authors: Wen-Hua
authors: WH
authors: Wang
authors: Xiao-Yan
authors: XY
authors: Zhang
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002555:Cerebrospinal Fluid; D008297:Male; D008516:Medicine, Chinese Traditional; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D016229:Amyloid beta-Peptides; C494828:Bax protein, rat; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D019253:Proto-Oncogene Proteins c-bcl-2; C074636:amyloid beta-protein (25-35); D051028:bcl-2-Associated X Protein; D053148:Caspase 3