title: Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes.
abstract: Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting.
authors: The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
authors: Ben
authors: B
authors: Spitzer-Rimon
authors: Alon
authors: A
authors: Cna'ani
authors: Alexander
authors: A
authors: Vainstein
mesh_terms: D016960:Agrobacterium tumefaciens / Q000821:virology; D001483:Base Sequence; D035264:Flowers / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D015870:Gene Expression; D055785:Gene Knockdown Techniques / Q000379:methods*; D017343:Genes, Plant; D005822:Genetic Vectors; D032306:Petunia / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010942:Plant Viruses / Q000235:genetics*; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014026:Nicotiana / Q000821:virology; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D011993:Recombinant Fusion Proteins; D055549:Volatile Organic Compounds