title: Purification of a novel peptide derived from Mytilus coruscus and in vitro/in vivo evaluation of its bioactive properties.
abstract: Excess oxidant can promote inflammatory responses. Moreover, chronic inflammation accompanied by oxidative stress is connected various steps involved in many diseases. From the aspect, we investigated an antioxidant peptide to prevent inflammatory response against oxidant overexpression. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis, and the antioxidant properties of the hydrolysates were investigated using free radical scavenging activity by electron spin resonance (ESR) spectrometry. Papain hydrolysates, which showed clearly superior free radical scavenging activity, were further purified using consecutive chromatographic methods. Finally, a novel antioxidant peptide was obtained, and the sequence was identified as Ser-Leu-Pro-Ile-Gly-Leu-Met-Ile-Ala-Met at N-terminal. Oral administration of the peptide to mice effectively inhibited malondialdehyde (MDA) levels in a thiobarbituric acid reactive substances (TBARS) assay, and we also confirmed the antioxidative enzyme activities in superoxide dismutase (SOD) and glutathione-s-transferase (GST) assays. This is the first report of an antioxidant peptide derived from the hydrolysate of Mytilus coruscus, and also these results suggest that the peptide possesses potent antioxidant activity, and potential to enhance anti-inflammatory response.
authors: Department of Natural Science, Konkuk University, Chungju 380-701, Republic of Korea.
authors: Eun-Kyung
authors: EK
authors: Kim
authors: Hyun-Jung
authors: HJ
authors: Oh
authors: Yon-Suk
authors: YS
authors: Kim
authors: Jin-Woo
authors: JW
authors: Hwang
authors: Chang-Bum
authors: CB
authors: Ahn
authors: Jung Suck
authors: JS
authors: Lee
authors: You-Jin
authors: YJ
authors: Jeon
authors: Sang-Ho
authors: SH
authors: Moon
authors: Si Heung
authors: SH
authors: Sung
authors: Byong-Tae
authors: BT
authors: Jeon
authors: Pyo-Jam
authors: PJ
authors: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D049878:Mytilus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D056910:Republic of Korea; D020539:Sequence Analysis, Protein; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010455:Peptides; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005982:Glutathione Transferase