title: Isolation of bovine corneal keratan sulfate and its growth factor and morphogen binding.
abstract: Keratan sulfate (KS) is an important glycosaminoglycan that is found in cartilage, reproductive tissues, and neural tissues. Corneal KS glycosaminoglycan is found N-linked to lumican, keratocan and mimecan proteoglycans, and has been widely studied by investigators interested in corneal development and diseases. Recently, the availability of corneal KS has become severely limited, owing to restrictions on the shipment of bovine central nervous system byproducts across international borders in an effort to prevent additional cases of mad cow disease. We report a simple method for the purification of multi-milligram quantities of bovine corneal KS, and characterize its structural properties. We also examined its protein-binding properties, and discovered that corneal KS bound with high affinity to fibroblast growth factor-2 and sonic hedgehog, a growth factor and a morphogen involved in corneal development and healing.
authors: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors: Amanda
authors: A
authors: Weyers
authors: Bo
authors: B
authors: Yang
authors: Kemal
authors: K
authors: Solakyildirim
authors: Vienna
authors: V
authors: Yee
authors: Lingyun
authors: L
authors: Li
authors: Fuming
authors: F
authors: Zhang
authors: Robert J
authors: RJ
authors: Linhardt
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003315:Cornea / Q000737:chemistry*; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry; D053823:Hedgehog Proteins / Q000737:chemistry*; D007632:Keratan Sulfate / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*
chemical_list: D053823:Hedgehog Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D007632:Keratan Sulfate
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