title: Species identification of medically important trematodes in aquatic food samples using PCR-RFLP targeting 18S rRNA.
abstract: The authors describe a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method targeting the 18S rRNA gene for the species detection of medically important trematode infecting fish and oysters, and suggest that this PCR-RFLP method based on a specific Tre-18 primer and the restriction enzymes, Acc1, Ava2, Msp1, and Hinf1, is useful for the detection of parasites in aquatic food.
authors: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Republic of Korea.
authors: Kyoung-Ho
authors: KH
authors: Pyo
authors: Eun-Young
authors: EY
authors: Kang
authors: Young-Sang
authors: YS
authors: Hwang
authors: Ho-Chong
authors: HC
authors: Jun
authors: Woon-Mok
authors: WM
authors: Sohn
authors: Shin-Hyeong
authors: SH
authors: Cho
authors: Won-Ja
authors: WJ
authors: Lee
authors: Jong-Yil
authors: JY
authors: Chai
authors: Eun-Hee
authors: EH
authors: Shin
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics; D018509:DNA, Helminth / Q000302:isolation & purification*; D005399:Fishes / Q000469:parasitology; D005506:Food Contamination / Q000032:analysis; D016266:Food Parasitology; D006376:Helminths / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D010125:Ostreidae / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000302:isolation & purification*; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D012337:RNA, Ribosomal, 18S; D004262:DNA Restriction Enzymes
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