title: Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis.
abstract: Several lipid-transfer proteins were reported to modulate the plant response to biotic stress; however, whether lipid-transfer proteins are also involved in abiotic stress remains unknown. This study characterized the function of a lipid-transfer protein, LTP3, during freezing and drought stress. LTP3 was expressed ubiquitously and the LTP3 protein was localized to the cytoplasm. A biochemical study showed that LTP3 was able to bind to lipids. Overexpression of LTP3 resulted in constitutively enhanced freezing tolerance without affecting the expression of CBFs and their target COR genes. Further analyses showed that LTP3 was positively regulated by MYB96 via the direct binding to the LTP3 promoter; consistently, transgenic plants overexpressing MYB96 exhibited enhanced freezing tolerance. This study also found that the loss-of-function mutant ltp3 was sensitive to drought stress, whereas overexpressing plants were drought tolerant, phenotypes reminiscent of myb96 mutant plants and MYB96-overexpressing plants. Taken together, these results demonstrate that LTP3 acts as a target of MYB96 to be involved in plant tolerance to freezing and drought stress.
authors: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors: Lin
authors: L
authors: Guo
authors: Haibian
authors: H
authors: Yang
authors: Xiaoyan
authors: X
authors: Zhang
authors: Shuhua
authors: S
authors: Yang
mesh_terms: D000064:Acclimatization; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D055864:Droughts; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011523:Protoplasts / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C544823:MYB96 protein, Arabidopsis; D014157:Transcription Factors; D049452:Green Fluorescent Proteins
references: Nat Methods. 2005 Mar;2(3):213-8
references: 16163802
references: Eur J Biochem. 1984 Mar 1;139(2):411-6
references: 6698022
references: Plant Cell. 2012 Jun;24(6):2578-95
references: 22706288
references: Nat Protoc. 2007;2(7):1565-72
references: 17585298
references: Endeavour. 1971 Sep;30(111):130-5
references: 4110469
references: Plant J. 2009 Apr;58(1):97-108
references: 19067974
references: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:541-568
references: 15012300
references: Curr Biol. 2005 Jul 12;15(13):1196-200
references: 16005291
references: Nat Biotechnol. 1999 Mar;17(3):287-91
references: 10096298
references: Nature. 2002 Sep 26;419(6905):399-403
references: 12353036
references: Plant Physiol. 2009 Sep;151(1):275-89
references: 19625633
references: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:627-654
references: 15012303
references: Plant Cell. 2006 Jul;18(7):1736-49
references: 16751345
references: Plant Cell. 2012 Jun;24(6):2546-61
references: 22730405
references: Eur J Biochem. 1998 Oct 15;257(2):443-8
references: 9826191
references: Plant J. 1997 Sep;12(3):669-75
references: 9351251
references: Plant Cell. 2005 Jan;17(1):256-67
references: 15598798
references: EMBO J. 2002 Jun 3;21(11):2692-702
references: 12032082
references: Plant J. 1998 Dec;16(6):735-43
references: 10069079
references: Prog Lipid Res. 2004 May;43(3):177-99
references: 15003394
references: Plant Cell. 1993 Nov;5(11):1529-39
references: 8312738
references: Genes Dev. 2003 Apr 15;17(8):1043-54
references: 12672693
references: Plant Physiol. 2008 Feb;146(2):623-35
references: 18162593
references: Plant Cell. 2009 Mar;21(3):972-84
references: 19270186
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
references: 15012220
references: Plant Physiol. 1997 Nov;115(3):875-879
references: 12223851
references: Annu Rev Plant Biol. 2006;57:781-803
references: 16669782
references: Plant Cell. 2000 Jun;12(6):853-70
references: 10852933
references: New Phytol. 2010 Apr;186(2):471-83
references: 20149112
references: Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4945-50
references: 18356294
references: Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14570-5
references: 9826741
references: Plant Cell. 2009 Aug;21(8):2527-40
references: 19717619
references: Plant J. 2010 Jul;63(2):283-96
references: 20444230
references: Plant Mol Biol. 2004 Mar;54(5):767-81
references: 15356394
references: Plant Physiol. 2010 Oct;154(2):796-809
references: 20699401
references: Plant Physiol. 1999 Jun;120(2):391-400
references: 10364390
references: Plant Cell. 2011 Mar;23(3):1138-52
references: 21398568
references: Plant Cell. 2009 Dec;21(12):3902-14
references: 20044438
references: Mol Cell Biochem. 1999 Feb;192(1-2):157-61
references: 10331670
references: Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13404-9
references: 11038526
references: Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7799-804
references: 9636231
references: Protein Sci. 2008 Feb;17(2):191-8
references: 18096636
references: Mol Cell Biol. 2006 Dec;26(24):9533-43
references: 17030626
references: Plant Cell. 2005 Jul;17(7):2009-19
references: 15937228
references: Proc Natl Acad Sci U S A. 2006 May 23;103(21):8281-6
references: 16702557
references: J Biol Chem. 2002 Aug 30;277(35):31994-2002
references: 12077151
references: Science. 1998 Apr 3;280(5360):104-6
references: 9525853
references: Mol Biol Cell. 2004 Nov;15(11):5047-52
references: 15356262
references: Plant J. 2000 Oct;24(2):265-73
references: 11069700
references: Plant Cell. 1998 Aug;10(8):1391-406
references: 9707537
references: Science. 2009 Apr 3;324(5923):89-91
references: 19342588
references: J Biol Chem. 2006 Dec 8;281(49):37636-45
references: 17015446
references: Plant Cell. 2007 Apr;19(4):1403-14
references: 17416732
references: Plant Cell. 1991 Sep;3(9):907-21
references: 1822991
references: Plant J. 1999 Apr;18(2):185-93
references: 10363370
references: Plant Cell. 2009 Apr;21(4):1230-8
references: 19366900