title: Transcriptome analysis of tomato flower pedicel tissues reveals abscission zone-specific modulation of key meristem activity genes.
abstract: Tomato flower abscises at the anatomically distinct abscission zone that separates the pedicel into basal and apical portions. During abscission, cell separation occurs only at the abscission zone indicating distinctive molecular regulation in its cells. We conducted a transcriptome analysis of tomato pedicel tissues during ethylene promoted abscission. We found that the abscission zone was the most active site with the largest set of differentially expressed genes when compared with basal and apical portions. Gene Ontology analyses revealed enriched transcription regulation and hydrolase activities in the abscission zone. We also demonstrate coordinated responses of hormone and cell wall related genes. Besides, a number of ESTs representing homologs of key Arabidopsis shoot apical meristem activity genes were found to be preferentially expressed in the abscission zone, including WUSCHEL (WUS), KNAT6, LATERAL ORGAN BOUNDARIES DOMAIN PROTEIN 1(LBD1), and BELL-like homeodomain protein 1 (BLH1), as well as tomato axillary meristem genes BLIND (Bl) and LATERAL SUPPRESSOR (Ls). More interestingly, the homologs of WUS and the potential functional partner OVATE FAMILIY PROTEIN (OFP) were subsequently down regulated during abscission while Bl and AGL12 were continuously and specifically induced in the abscission zone. The expression patterns of meristem activity genes corroborate the idea that cells of the abscission zone confer meristem-like nature and coincide with the course of abscission and post-abscission cell differentiation. Our data therefore propose a possible regulatory scheme in tomato involving meristem genes that may be required not only for the abscission zone development, but also for abscission.
authors: National Center for Wheat Research, Henan Agricultural University, Zhengzhou, People's Republic of China.
authors: Xiang
authors: X
authors: Wang
authors: Danmei
authors: D
authors: Liu
authors: Aili
authors: A
authors: Li
authors: Xiuli
authors: X
authors: Sun
authors: Rongzhi
authors: R
authors: Zhang
authors: Liang
authors: L
authors: Wu
authors: Yanchun
authors: Y
authors: Liang
authors: Long
authors: L
authors: Mao
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D005030:Ethylenes / Q000494:pharmacology; D035264:Flowers / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005638:Fruit / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D018519:Meristem / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects
chemical_list: D005030:Ethylenes; D018398:Homeodomain Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012097:Repressor Proteins; C036216:ethylene; D006867:Hydrolases
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