title: Expression and characterisation of recombinant molecules in transgenic soybean.
abstract: Seeds are organs specialised in accumulating proteins, and they may provide a potential economically viable platform for the large-scale production and storage of many molecules for pharmaceutical and other productive sectors. Soybean [Glycine max (L.) Merrill] has a high seed protein content and represents an excellent source of abundant and cheap biomass. Under greenhouse conditions and a daily photoperiod of 23 h of light, the soybean plant's vegetative growth can be significantly extended by inducing more than a tenfold increase in seed production when compared with plants cultivated under field conditions. Some factors involved in the production of different recombinant proteins in soybean seeds are discussed in this review. These include transgenic system, regulatory sequences and the use of Mass Spectrometry as a new tool for molecular characterisation of seed produced recombinant proteins. The important intrinsic characteristics and possibility of genetically engineering soybean seeds, using current advances in recombinant DNA technology including metabolic engineering and synthetic biology, should form the foundation for large-scale and more precise genome modification, making this crop an important candidate as bioreactor for production of recombinant molecules.
authors: Embrapa Genetic Resources and Biotechnology. Synthetic Biology and Nanotechnology Group. Parque Estacao Biologica, Brasilia, DF, 70770-917, Brazil.
authors: Nicolau B
authors: NB
authors: da Cunha
authors: Andre M
authors: AM
authors: Murad
authors: Giovanni R
authors: GR
authors: Vianna
authors: Cintia
authors: C
authors: Coelho
authors: Elibio L
authors: EL
authors: Rech
mesh_terms: D000818:Animals; D005818:Genetic Engineering / Q000379:methods; D018745:Genome, Plant; D006801:Humans; D013058:Mass Spectrometry; D058634:Molecular Farming / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics*; D012639:Seeds; D030262:Soybean Proteins / Q000235:genetics*; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019076:Transgenes
chemical_list: D011994:Recombinant Proteins; D030262:Soybean Proteins