title: Porous decellularized adipose tissue foams for soft tissue regeneration.
abstract: To design tissue-specific bioscaffolds with well-defined properties and 3-D architecture, methods were developed for preparing porous foams from enzyme-solubilized human decellularized adipose tissue (DAT). Additionally, a technique was established for fabricating "bead foams" comprised of interconnected networks of porous DAT beads fused through a controlled freeze-thawing and lyophilization procedure. In characterization studies, the foams were stable without the need for chemical crosslinking, with properties that could be tuned by controlling the protein concentration and freezing rate during synthesis. Adipogenic differentiation studies with human adipose-derived stem cells (ASCs) suggested that stiffness influenced ASC adipogenesis on the foams. In support of our previous work with DAT scaffolds and microcarriers, the DAT foams and bead foams strongly supported adipogenesis and were also adipo-inductive, as demonstrated by glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, endpoint RT-PCR analysis of adipogenic gene expression, and intracellular lipid accumulation. Adipogenic differentiation was enhanced on the microporous DAT foams, potentially due to increased cell-cell interactions in this group. In vivo assessment in a subcutaneous Wistar rat model demonstrated that the DAT bioscaffolds were well tolerated and integrated into the host tissues, supporting angiogenesis and adipogenesis. The DAT-based foams induced a strong angiogenic response, promoted inflammatory cell migration and gradually resorbed over the course of 12 weeks, demonstrating potential as scaffolds for wound healing and soft tissue regeneration.
authors: Department of Chemical Engineering, Queen's University, 19 Division St., Kingston, ON, Canada K7L 3N6.
authors: Claire
authors: C
authors: Yu
authors: Juares
authors: J
authors: Bianco
authors: Cody
authors: C
authors: Brown
authors: Lydia
authors: L
authors: Fuetterer
authors: John F
authors: JF
authors: Watkins
authors: Abbas
authors: A
authors: Samani
authors: Lauren E
authors: LE
authors: Flynn
mesh_terms: D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D055119:Elastic Modulus / Q000187:drug effects; D005260:Female; D005612:Freeze Drying; D005786:Gene Expression Regulation / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D055595:Mechanical Phenomena; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D023421:Models, Animal; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D040521:Subcutaneous Tissue / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005993:Glycerolphosphate Dehydrogenase