title: Phage Lambda P protein: trans-activation, inhibition phenotypes and their suppression.
abstract: The initiation of bacteriophage λ replication depends upon interactions between the oriλ DNA site, phage proteins O and P, and E. coli host replication proteins. P exhibits a high affinity for DnaB, the major replicative helicase for unwinding double stranded DNA. The concept of P-lethality relates to the hypothesis that P can sequester DnaB and in turn prevent cellular replication initiation from oriC. Alternatively, it was suggested that  P-lethality does not involve an interaction between P and DnaB, but is targeted to DnaA.  P-lethality is assessed by examining host cells for transformation by ColE1-type plasmids that can express P, and the absence of transformants is attributed to a lethal effect of P expression. The plasmid we employed enabled conditional expression of P, where under permissive conditions, cells were efficiently transformed. We observed that ColE1 replication and plasmid establishment upon transformation is extremely sensitive to P, and distinguish this effect from P-lethality directed to cells. We show that alleles of dnaB protect the variant cells from P expression. P-dependent cellular filamentation arose in ΔrecA or lexA[Ind-] cells, defective for SOS induction. Replication propagation and restart could represent additional targets for P interference of E. coli replication, beyond the  oriC-dependent initiation step.
authors: Department of Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, S7N 5E5, Canada. sidney.hayes@usask.ca
authors: Sidney
authors: S
authors: Hayes
authors: Craig
authors: C
authors: Erker
authors: Monique A
authors: MA
authors: Horbay
authors: Kristen
authors: K
authors: Marciniuk
authors: Wen
authors: W
authors: Wang
authors: Connie
authors: C
authors: Hayes
mesh_terms: D000483:Alleles; D010582:Bacteriophage lambda / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D023061:Gene Order; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D013014:SOS Response, Genetics; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014169:Transformation, Bacterial; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C024013:DNA replication complex protein, Bacteriophage lambda; D015534:Trans-Activators; D014764:Viral Proteins
references: Proc Natl Acad Sci U S A. 1977 Dec;74(12):5265-9
references: 271952
references: J Biol Chem. 1978 Jun 10;253(11):4043-50
references: 206559
references: Microbiol Mol Biol Rev. 1998 Jun;62(2):434-64
references: 9618448
references: Nucleic Acids Res. 1981 Apr 24;9(8):1789-99
references: 6264392
references: Gene. 2003 Dec 4;321:163-71
references: 14637004
references: J Mol Biol. 1977 Jun 15;113(1):1-25
references: 328896
references: J Virol. 1986 Jun;58(3):835-42
references: 2939262
references: Virology. 1974 Mar;58(1):313-5
references: 4821702
references: Genetics. 2005 Aug;170(4):1485-99
references: 15956667
references: J Mol Biol. 1978 Mar 25;120(1):137-43
references: 642005
references: Trends Biochem Sci. 2003 Mar;28(3):116-8
references: 12633988
references: Nucleic Acids Res. 2002 Jul 1;30(13):2809-16
references: 12087164
references: Cell. 1987 Jan 16;48(1):73-8
references: 3791416
references: Nucleic Acids Res. 1981 Aug 25;9(16):4149-61
references: 6272195
references: Mol Gen Genet. 1983;192(3):430-5
references: 6318039
references: J Biol Chem. 1981 May 25;256(10):5253-9
references: 6262325
references: J Biochem Mol Biol. 2005 Jan 31;38(1):89-96
references: 15715952
references: Cell. 1987 Dec 24;51(6):1113-22
references: 2446774
references: Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4
references: 6156450
references: Mol Gen Genet. 1979 Mar 20;171(2):219-27
references: 375032
references: J Bacteriol. 1989 Nov;171(11):6030-8
references: 2681151
references: J Biol Chem. 1998 Dec 18;273(51):34255-62
references: 9852089
references: Cell. 1981 Dec;27(3 Pt 2):515-22
references: 6101204
references: J Bacteriol. 1997 Mar;179(6):1931-9
references: 9068638
references: J Bacteriol. 1989 Aug;171(8):4303-14
references: 2666394
references: Vaccine. 2010 Sep 24;28(41):6789-99
references: 20674873
references: J Bacteriol. 1986 Apr;166(1):143-7
references: 2870052
references: Mol Gen Genet. 1976 Aug 2;146(3):299-302
references: 1004487
references: Cell. 1990 Jul 27;62(2):331-8
references: 1695550
references: Mol Cell. 2002 Sep;10(3):647-57
references: 12408831
references: Proc Natl Acad Sci U S A. 1975 Mar;72(3):921-5
references: 1093174
references: J Bacteriol. 1977 Mar;129(3):1476-86
references: 321430
references: Mol Gen Genet. 1996 Oct 28;252(6):755-60
references: 8917321
references: J Bacteriol. 1981 Oct;148(1):265-73
references: 7026534
references: Nucleic Acids Res. 1990 Aug 25;18(16):4809-15
references: 1697676
references: Nucleic Acids Res. 2011 Sep 1;39(16):7020-33
references: 21622658
references: Nucleic Acids Res. 1982 Feb 11;10(3):833-53
references: 6278438
references: Mol Gen Genet. 1977 Feb 28;151(1):35-9
references: 325365
references: Microbiol Rev. 1984 Mar;48(1):60-93
references: 6371470
references: J Biol Chem. 1988 Feb 25;263(6):2719-23
references: 2830261
references: J Biochem. 1983 Aug;94(2):331-8
references: 6226650
references: J Bacteriol. 1984 May;158(2):551-61
references: 6327610
references: J Biol Chem. 1984 Jan 10;259(1):97-101
references: 6323420
references: Proc Natl Acad Sci U S A. 1980 Nov;77(11):6566-70
references: 7005899
references: Cell. 1988 Mar 11;52(5):743-55
references: 2830993
references: Trends Biochem Sci. 2000 Apr;25(4):185-9
references: 10754552
references: Proc Natl Acad Sci U S A. 1975 Mar;72(3):1050-4
references: 1093162
references: J Mol Biol. 1968 Jul 14;35(1):175-92
references: 4939776
references: Nucleic Acids Res. 1979 Mar;6(3):867-81
references: 375198
references: Curr Biol. 1999 Feb 11;9(3):151-4
references: 10021390
references: J Biol Chem. 1978 Jan 10;253(1):261-9
references: 145439
references: Mol Gen Genet. 1990 Jun;222(1):17-24
references: 2146486
references: Eur J Biochem. 1980 Mar;105(1):1-6
references: 6245874
references: Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4
references: 4601821
references: Gene. 1998 Nov 26;223(1-2):115-28
references: 9858705
references: Front Biosci. 1996 Mar 01;1:d48-58
references: 9159210
references: EMBO J. 1989 May;8(5):1601-8
references: 2527744
references: Mol Gen Genet. 1978 Aug 4;164(1):63-76
references: 703760
references: Mol Gen Genet. 1973 Nov 22;126(4):275-90
references: 4784811
references: Gene. 1997 Apr 11;189(1):35-42
references: 9161409
references: J Biol Chem. 1995 Apr 28;270(17):9792-9
references: 7730358
references: J Biol Chem. 1990 Sep 5;265(25):15124-33
references: 2144283
references: J Virol. 1983 Nov;48(2):551-4
references: 6312108
references: Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:303-10
references: 225103
references: J Biol Chem. 1987 Jun 5;262(16):7831-8
references: 3034907
references: EMBO J. 2002 Dec 16;21(24):6944-53
references: 12486015
references: J Proteome Res. 2004 May-Jun;3(3):463-8
references: 15253427
references: Proc Natl Acad Sci U S A. 1977 Oct;74(10):4190-4
references: 144914
references: J Biol Chem. 2001 Feb 9;276(6):4441-6
references: 11078744
references: Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12902-7
references: 8917517
references: PLoS One. 2012;7(5):e36498
references: 22590552
references: J Mol Biol. 1970 Apr 28;49(2):393-404
references: 5447824
references: Arch Microbiol. 2000 Jul-Aug;174(1-2):89-96
references: 10985747
references: Virology. 1991 May;182(1):351-2
references: 1827225
references: Mol Gen Genet. 1977 Feb 28;151(1):27-34
references: 325364
references: J Bacteriol. 1993 Dec;175(23):7720-3
references: 8244945
references: Proc Natl Acad Sci U S A. 1977 Feb;74(2):689-93
references: 322139
references: Proc Natl Acad Sci U S A. 1977 May;74(5):1865-9
references: 325558
references: J Biol Chem. 1990 Aug 5;265(22):13297-307
references: 2165499
references: Mol Gen Genet. 1982;187(1):79-86
references: 6219272
references: Mol Microbiol. 2010 Jul;77(2):324-36
references: 20497334
references: Can J Microbiol. 2005 Jan;51(1):37-49
references: 15782233
references: J Biol Chem. 1986 Apr 5;261(10):4738-48
references: 3007474
references: J Biol Chem. 1985 Aug 5;260(16):9316-25
references: 2991240
references: Genes Dev. 1990 Dec;4(12A):2223-33
references: 2148537
references: J Biochem Mol Biol. 2005 Jan 31;38(1):97-103
references: 15715953
references: J Biol Chem. 1989 Jun 25;264(18):10699-708
references: 2525129
references: Virology. 1991 May;182(1):324-35
references: 1827224
references: Science. 1994 Apr 8;264(5156):258-60
references: 8146657
references: Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8203-10
references: 11459954
references: Proc Natl Acad Sci U S A. 1984 Dec;81(24):7845-9
references: 6096862
references: Gene. 1982 Nov;20(1):11-24
references: 6219041
references: Nature. 1975 Sep 18;257(5523):253-4
references: 1099459