title: In vivo activity of nisin A and nisin V against Listeria monocytogenes in mice.
abstract: BACKGROUND
  Lantibiotics are post-translationally modified antimicrobial peptides, of which nisin A is the most extensively studied example. Bioengineering of nisin A has resulted in the generation of derivatives with increased in vitro potency against Gram-positive bacteria. Of these, nisin V (containing a Met21Val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including Listeria monocytogenes. However, this increased potency has not been tested in vivo.
  
  
  RESULTS
  Here we address this issue by assessing the ability of nisin A and nisin V to control a bioluminescent strain of Listeria monocytogenes EGDe in a murine infection model.More specifically, Balb/c mice were infected via the intraperitoneal route at a dose of 1 × 10(5) cfu/animal and subsequently treated intraperitoneally with either nisin V, nisin A or a PBS control. Bioimaging of the mice was carried out on day 3 of the trial. Animals were then sacrificed and levels of infection were quantified in the liver and spleen.
  
  
  CONCLUSIONS
  This analysis revealed that nisin V was more effective than Nisin A with respect to controlling infection and therefore merits further investigation with a view to potential chemotherapeutic applications.
authors: Department of Microbiology, University College Cork, Cork, Ireland.
authors: Alicia
authors: A
authors: Campion
authors: Pat G
authors: PG
authors: Casey
authors: Des
authors: D
authors: Field
authors: Paul D
authors: PD
authors: Cotter
authors: Colin
authors: C
authors: Hill
authors: R Paul
authors: RP
authors: Ross
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D008089:Listeria monocytogenes / Q000187:drug effects*; D008088:Listeriosis / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D009561:Nisin / Q000008:administration & dosage* / Q000494:pharmacology; D016896:Treatment Outcome; D051598:Whole Body Imaging
chemical_list: D000900:Anti-Bacterial Agents; D009561:Nisin
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