title: Immunorelevant proteins for the diagnosis of bovine staphylococcal mastitis.
abstract: Staphylococcus aureus is a leading cause of bovine mastitis, a condition in which the udder of the cow is inflamed, reducing the quality and quantity of milk produced. Staphylococcal mastitis is a common infection that can develop into a chronic form. The segregation of infected animals is an important preventive practice but relies on an effective diagnostic method. For this purpose, we constructed a genomic library of S. aureus, and a screening step was conducted with antiserum produced using the total protein extract of the pathogen. The nucleotide sequences of the immunoselected clones were aligned with the genome of bovine S. aureus RF122, which enabled the identification of 65 different loci, including proteins related to metabolism, adhesion and cell wall production, toxins, regulatory proteins, and hypothetical proteins. The subcellular location of the immunoreactive polypeptides was also determined. Fifty-two percent were cytoplasmic, 34 % were located in areas exposed to the host's immune system, and for 14 %, the location could not be determined. In silico analysis of the presence of these proteins in mastitis pathogens showed that Fib, ClfA, and the hypothetical protein SAB0166 were the only proteins specific for S. aureus. Therefore, these proteins are promising candidates for the serodiagnosis of staphylococcal mastitis.
authors: Laboratory of Molecular Biotechnology, Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Brazil.
authors: M H
authors: MH
authors: Fabres-Klein
authors: R C
authors: RC
authors: Klein
authors: S O
authors: SO
authors: De Paula
authors: A O B
authors: AO
authors: Ribon
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D002417:Cattle; D008414:Mastitis, Bovine / Q000175:diagnosis*; D012698:Serologic Tests / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins
references: Proteomics. 2006 Mar;6(5):1530-49
references: 16470658
references: J Dairy Sci. 2008 Nov;91(11):4244-8
references: 18946129
references: Microb Pathog. 2006 Apr;40(4):177-83
references: 16517115
references: Clin Diagn Lab Immunol. 2005 Apr;12(4):484-90
references: 15817754
references: J Infect Dis. 2001 Dec 15;184(12):1572-80
references: 11740733
references: J Appl Microbiol. 2000 Jun;88(6):1028-37
references: 10849179
references: Proc Natl Acad Sci U S A. 2002 May 14;99(10):6573-8
references: 11997460
references: Vet Res. 2011 Feb 15;42:35
references: 21324116
references: Int J Med Microbiol. 2010 Feb;300(2-3):176-92
references: 19889576
references: Scand J Immunol. 2008 Feb;67(2):169-76
references: 18201371
references: Vet Microbiol. 2010 Oct 26;145(3-4):360-5
references: 20399576
references: Infect Immun. 2003 Aug;71(8):4633-41
references: 12874343
references: Vaccine. 2004 Nov 15;23(1):114-26
references: 15519714
references: Vaccine. 1994 Aug;12(11):988-92
references: 7975852
references: J Dairy Sci. 2008 Dec;91(12):4592-8
references: 19038934
references: Bioinformatics. 2010 Jul 1;26(13):1608-15
references: 20472543
references: Cell Microbiol. 2007 May;9(5):1172-90
references: 17217429
references: Vet Microbiol. 2012 Nov 9;160(1-2):183-8
references: 22709438
references: Wei Sheng Yan Jiu. 2007 Mar;36(2):183-6
references: 17555096
references: Appl Microbiol Biotechnol. 2008 Sep;80(3):447-58
references: 18636254
references: Clin Vaccine Immunol. 2008 Mar;15(3):513-21
references: 18175801
references: PLoS One. 2007 Oct 31;2(10):e1120
references: 17971880
references: Vet Clin North Am Food Anim Pract. 2003 Mar;19(1):171-85, vii
references: 12682941
references: Infect Immun. 2009 Jul;77(7):2719-29
references: 19364833
references: Infect Immun. 2006 Aug;74(8):4644-54
references: 16861652
references: Infect Immun. 2008 Dec;76(12):5738-44
references: 18809660
references: J Infect Dis. 2006 Apr 15;193(8):1098-108
references: 16544250
references: Mol Microbiol. 1994 May;12(4):599-606
references: 7934883
references: Vet Microbiol. 2009 Mar 2;134(3-4):388-91
references: 18835110
references: Trends Biotechnol. 2009 Aug;27(8):486-93
references: 19616330
references: Scand J Immunol. 2006 Mar;63(3):184-90
references: 16499571
references: Vet Microbiol. 2003 Mar 20;92(1-2):179-85
references: 12488081
references: J Dairy Sci. 2006 Jun;89(6):1877-95
references: 16702252
references: J Biol Chem. 2007 Oct 12;282(41):30051-61
references: 17699522
references: Trends Genet. 1995 Jun;11(6):217-8
references: 7638902
references: Curr Opin Microbiol. 2006 Feb;9(1):5-11
references: 16406780