title: Effects of brain-derived neurotrophic factor (BDNF) and electrical stimulation on survival and function of cochlear spiral ganglion neurons in deafened, developing cats.
abstract: Both neurotrophic support and neural activity are required for normal postnatal development and survival of cochlear spiral ganglion (SG) neurons. Previous studies in neonatally deafened cats demonstrated that electrical stimulation (ES) from a cochlear implant can promote improved SG survival but does not completely prevent progressive neural degeneration. Neurotrophic agents combined with an implant may further improve neural survival. Short-term studies in rodents have shown that brain-derived neurotrophic factor (BDNF) promotes SG survival after deafness and may be additive to trophic effects of stimulation. Our recent study in neonatally deafened cats provided the first evidence of BDNF neurotrophic effects in the developing auditory system over a prolonged duration Leake et al. (J Comp Neurol 519:1526-1545, 2011). Ten weeks of intracochlear BDNF infusion starting at 4 weeks of age elicited significant improvement in SG survival and larger soma size compared to contralateral. In the present study, the same deafening and BDNF infusion procedures were combined with several months of ES from an implant. After combined BDNF + ES, a highly significant increase in SG numerical density (>50 % improvement re: contralateral) was observed, which was significantly greater than the neurotrophic effect seen with ES-only over comparable durations. Combined BDNF + ES also resulted in a higher density of myelinated radial nerve fibers within the osseous spiral lamina. However, substantial ectopic and disorganized sprouting of these fibers into the scala tympani also occurred, which may be deleterious to implant function. EABR thresholds improved (re: initial thresholds at time of implantation) on the chronically stimulated channels of the implant. Terminal electrophysiological studies recording in the inferior colliculus (IC) revealed that the basic cochleotopic organization was intact in the midbrain in all studied groups. In deafened controls or after ES-only, lower IC thresholds were correlated with more selective activation widths as expected, but no such correlation was seen after BDNF + ES due to much greater variability in both measures.
authors: Epstein Hearing Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, University of California San Francisco, 533 Parnassus Ave., San Francisco, CA 94143-0526, USA. pleake@ohns.ucsf.edu
authors: Patricia A
authors: PA
authors: Leake
authors: Olga
authors: O
authors: Stakhovskaya
authors: Alexander
authors: A
authors: Hetherington
authors: Stephen J
authors: SJ
authors: Rebscher
authors: Ben
authors: B
authors: Bonham
mesh_terms: D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002415:Cats; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003051:Cochlea / Q000254:growth & development / Q000294:innervation* / Q000503:physiopathology; D003054:Cochlear Implants; D003638:Deafness / Q000503:physiopathology* / Q000628:therapy*; D004195:Disease Models, Animal; D004599:Electric Stimulation Therapy; D055724:Electrophysiological Phenomena / Q000187:drug effects / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013136:Spiral Ganglion / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor
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