title: Evaluation of B cell function in patients with HIV.
abstract: HIV disease is associated with abnormalities in all major lymphocyte populations, including B cells. B cell dysfunction in HIV infection is largely driven by alterations in the subsets of B cells that circulate in the blood or reside in tissues. Meaningful functional assays are thus dependent on the identification and isolation of B cell subsets present in the starting material. This unit describes several assays designed to phenotype, fractionate, and assess functional properties of B cells that circulate in the blood of HIV-infected individuals. The four protocols, which have been adapted from standard techniques, are tailored to evaluate B cells in peripheral blood mononuclear cells (PBMCs) of individuals infected with HIV, but can also be applied to other disease settings and PBMCs isolated from healthy individuals.
authors: National Institutes of Health, Bethesda, Maryland, USA.
authors: Clarisa M
authors: CM
authors: Buckner
authors: Lela
authors: L
authors: Kardava
authors: Susan
authors: S
authors: Moir
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001775:Blood Circulation / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D016867:Immunocompromised Host; D016130:Immunophenotyping / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D016131:Lymphocyte Subsets / Q000502:physiology*