title: Analyses of programmed cell death in dendritic cells.
abstract: Dendritic cells (DCs) are professional antigen-presenting cells that can regulate both innate and adaptive immune responses. Programmed cell death of DCs plays an important role in maintaining the homeostasis of DCs and in the regulation of immune responses. Methods to measure the rate of spontaneous and T cell-mediated cell death of DCs are described here. These procedures can be adapted to study the -induction of dendritic cell death in different settings of immune responses.
authors: Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, USA.
authors: Min
authors: M
authors: Chen
authors: Lily
authors: L
authors: Huang
authors: Jin
authors: J
authors: Wang
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D001973:Bromodeoxyuridine / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002469:Cell Separation; D003584:Cytological Techniques / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D010455:Peptides / Q000276:immunology; D013154:Spleen / Q000166:cytology
chemical_list: D010455:Peptides; D001973:Bromodeoxyuridine
references: Science. 2006 Feb 24;311(5764):1160-4
references: 16497935
references: Blood. 2002 Sep 1;100(5):1734-41
references: 12176895
references: J Exp Med. 2002 Nov 18;196(10):1307-19
references: 12438422
references: Mol Immunol. 2007 Apr;44(10):2558-65
references: 17267035
references: Immunity. 2007 May;26(5):629-41
references: 17509906