title: [Molecular pathology of the lungs. New perspectives by next generation sequencing].
abstract: Lung cancer is one of the most frequent malignancies in the western world. Its frequent association with a wide spectrum of mutations in genes encoding various signal transducers that are often linked to therapy response, emphasizes the obvious need for improved, fast and highly efficient approaches in molecular pathology. Comprehensive analyses of the mutation status of progression and therapy relevant genes can be performed by the novel sequencing forms named next generation sequencing (NGS) providing extremely high capacities for ultra-deep sequence analyses. The 454 pyrosequencing method, the sequencing by synthesis and the semiconductor sequencing platform are now available for parallel sequencing approaches of multitudinous target genes linked to multiple tumor DNA applications. The "one molecule, one clone, one read" principle by the NGS approaches supplies not only information on allele frequencies and mutation rates but also has the advantage of a very sensitive detection of low frequency variants.
authors: Institut für Pathologie, Universitätsklinik zu Köln, Kerpener Str. 62, 50924, Köln, Deutschland.
authors: C
authors: C
authors: Vollbrecht
authors: K
authors: K
authors: König
authors: L
authors: L
authors: Heukamp
authors: R
authors: R
authors: Büttner
authors: M
authors: M
authors: Odenthal
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D004252:DNA Mutational Analysis / Q000379:methods / Q000639:trends; D018450:Disease Progression; D066246:ErbB Receptors / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000639:trends*; D006801:Humans; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D057089:Pathology, Molecular / Q000193:education* / Q000639:trends*; D057285:Precision Medicine / Q000379:methods / Q000639:trends; D011379:Prognosis; D015398:Signal Transduction / Q000235:genetics
chemical_list: D005819:Genetic Markers; C512478:EGFR protein, human; D066246:ErbB Receptors
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