title: Virus-induced gene silencing for rice using agroinoculation.
abstract: Virus-induced gene silencing (VIGS) is a reverse genetics technique that is based on the RNA-mediated defense against viruses in plants. VIGS is a method of gene knockdown triggered by a replicating viral nucleic acid engineered to carry a host gene to be silenced. While there are a number of excellent VIGS vectors available for dicots, only a few are available for monocots. Here, we describe the detailed method of the use of a newly developed VIGS vector for rice, based on the rice-infecting Rice tungro bacilliform virus, a pararetrovirus with dsDNA genome. Using a method based on Agrobacterium-mediated injection of the VIGS construct at the meristematic region of young rice plants, silencing of target genes can be achieved and the silenced phenotype can be visualized in 3 weeks.
authors: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
authors: Arunima
authors: A
authors: Purkayastha
authors: Shweta
authors: S
authors: Sharma
authors: Indranil
authors: I
authors: Dasgupta
mesh_terms: D016960:Agrobacterium tumefaciens / Q000821:virology*; D003001:Cloning, Molecular; D055785:Gene Knockdown Techniques / Q000379:methods*; D005822:Genetic Vectors; D012275:Oryza / Q000235:genetics* / Q000821:virology; D034622:RNA Interference; D014169:Transformation, Bacterial; D052641:Tungrovirus / Q000235:genetics*