title: The oncogenic role of microRNA-130a/301a/454 in human colorectal cancer via targeting Smad4 expression.
abstract: Transforming growth factor (TGF)-β/Smad signaling plays an important role in colon cancer development, progression and metastasis. In this study we demonstrated that the microRNA-130a/301a/454 family is up-regulated in colon cancer tissues compared to paired adjacent normal mucosa, which share the same 3'-untranslational region (3'-UTR) binding seed sequence and are predicated to target Smad4. In colorectal cancer HCT116 and SW480 cells, overexpression of miRNA-130a/301a/454 mimics enhances cell proliferation and migration, while inhibitors of these miRNAs affect cell survival. The biological function of miRNA-130a/301a/454 on colon cancer cells is likely mediated by suppression of Smad4, and the up-regulation of the miRNAs is correlated with Smad4 down-regulation in human colon cancers. Collectively, these results suggest that miRNA-130a/301a/454 are novel oncogenic miRNAs contributing to colon tumorigenesis by regulating TGF-β/Smad signaling, which may have potential application in cancer therapy.
authors: Department of General Surgery, PLA General Hospital, Beijing, China.
authors: Lin
authors: L
authors: Liu
authors: Jing
authors: J
authors: Nie
authors: Lin
authors: L
authors: Chen
authors: Guanglong
authors: G
authors: Dong
authors: Xiaohui
authors: X
authors: Du
authors: Xin
authors: X
authors: Wu
authors: Yun
authors: Y
authors: Tang
authors: Weidong
authors: W
authors: Han
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D051901:Smad4 Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D051901:Smad4 Protein
references: Blood. 2008 Feb 1;111(3):1217-26
references: 17957028
references: Am J Med Sci. 2010 Nov;340(5):385-8
references: 20625274
references: J Biol Chem. 2010 Jul 23;285(30):22818-30
references: 20484049
references: Lancet. 2005 Jan 8-14;365(9454):153-65
references: 15639298
references: PLoS Biol. 2011 Dec;9(12):e1001214
references: 22162950
references: Proc Natl Acad Sci U S A. 2012 May 15;109(20):E1248-57
references: 22517757
references: Nat Biotechnol. 2009 May;27(5):459-61
references: 19363475
references: World J Gastroenterol. 2009 May 7;15(17):2089-96
references: 19418581
references: Science. 2005 Sep 2;309(5740):1519-24
references: 16141061
references: Cancer Res. 2011 Apr 15;71(8):2926-37
references: 21393507
references: EMBO J. 2011 Jan 5;30(1):57-67
references: 21113131
references: Gastroenterology. 2010 Mar;138(3):969-80.e1-3
references: 19909744
references: Nat Rev Cancer. 2006 Nov;6(11):857-66
references: 17060945
references: Oncol Rep. 2012 Feb;27(2):559-66
references: 22020746
references: CA Cancer J Clin. 2007 Jan-Feb;57(1):43-66
references: 17237035
references: EMBO Rep. 2011 Oct 28;12(11):1153-9
references: 21941297
references: Cell Stem Cell. 2010 Jul 2;7(1):51-63
references: 20621050
references: Carcinogenesis. 2012 Jan;33(1):220-5
references: 22072615
references: Mol Cancer. 2009 Nov 14;8:102
references: 19912656
references: Cell. 2006 Aug 25;126(4):663-76
references: 16904174
references: Cell. 2003 Apr 4;113(1):25-36
references: 12679032
references: Science. 2007 Apr 27;316(5824):604-8
references: 17463289
references: Cancer Discov. 2012 Aug;2(8):736-49
references: 22750848
references: Int J Oncol. 2009 Apr;34(4):1069-75
references: 19287964
references: Mol Cancer. 2006 Jul 19;5:29
references: 16854228
references: J Cell Commun Signal. 2011 Aug;5(3):183-91
references: 21638198
references: RNA. 2005 Dec;11(12):1753-61
references: 16314451
references: Nature. 2004 Nov 11;432(7014):226-30
references: 15538371
references: Biochem J. 2011 Mar 15;434(3):473-82
references: 21175428
references: Dig Dis Sci. 2012 May;57(5):1171-80
references: 22373864
references: Nucleic Acids Res. 2011 Feb;39(3):1054-65
references: 20870751
references: J Dig Dis. 2010 Feb;11(1):50-4
references: 20132431
references: Cancer Res. 2012 Apr 1;72(7):1763-72
references: 22350415
references: Oncogene. 1999 May 20;18(20):3098-103
references: 10340381
references: Biocell. 2011 Aug;35(2):43-9
references: 22128589
references: J Cell Biochem. 2012 Oct;113(10):3229-35
references: 22628193
references: Biochem Biophys Res Commun. 2010 Sep 17;400(2):236-40
references: 20727858
references: Cancer Res. 1998 Mar 15;58(6):1124-6
references: 9515793
references: Cell Stem Cell. 2011 Apr 8;8(4):376-88
references: 21474102
references: Nucleic Acids Res. 2012 Oct;40(18):9286-97
references: 22821565