title: [Simultaneous determination of nine heterocyclic aromatic amines in mutton products by solid phase extraction-high performance liquid chromatography].
abstract: A method was established for the simultaneous determination of nine heterocyclic aromatic amines (HAAs) in mutton products by solid phase extraction-high performance liquid chromatography (SPE-HPLC). As a result, the sample was prepared by ultrasound in 2 mol/L NaOH, and dichloromethane was selected as the extraction solvent. The extract was purified and concentrated with an MCX SPE column. The chromatographic separation was achieved on a reverse-phase C18 column by gradient elution using 0.01 mol/L phosphoric acid (adjusted to pH 3.6 by triethylamine) and acetonitrile, detected with a diode array detector (DAD) at 228 nm for 2-amino-9H-pyrido [ 2,3-b] indole (AaC) and 2-amino-3-methyl-9H-pyrido [ 2,3-b] indole (MeAaC); 253 nm for 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 1-methyl-9H-pyrido[3, 4-b] indole (Harman), 9H-pyrido[3,4-b] indole (Norharman); 263 nm for 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo [4,5-f] quinoxaline (4, 8-DiMeIQx), 3-amino-1-methyl-5H-pyrido [4,3-b] indole (Trp-p-2); 321 nm for 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP). The results showed that the nine HAAs can be separated efficiently. The recoveries (n = 6) of the nine HAAs spiked in meat were between 50.27% and 94.77% with the relative standard deviations of 0.08%-4.42%. The limits of detection of this method were 1.6 -41.0 microg/L for the nine HAAs. The method is simple, accurate, rapid and repeatable. It can be used for the simultaneous determination of the nine HAAs in mutton products.
authors: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agricultural Product Processing and Quality Control, Ministry of Agriculture, Beijing 100193, China.
authors: Haitao
authors: H
authors: Guo
authors: Han
authors: H
authors: Pan
authors: Zhenyu
authors: Z
authors: Wang
authors: Li
authors: L
authors: Chen
authors: Dequan
authors: D
authors: Zhang
mesh_terms: D000588:Amines / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006571:Heterocyclic Compounds / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D012756:Sheep; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D000588:Amines; D006571:Heterocyclic Compounds