title: Enhanced bone-forming activity of side population cells in the periodontal ligament.
abstract: Regeneration of alveolar bone is critical for the successful treatment of periodontal diseases. The periodontal ligament (PDL) has been widely investigated as a source of cells for the regeneration of periodontal tissues. In the present study where we attempted to develop an effective strategy for alveolar bone regeneration, we examined the osteogenic potential of side population (SP) cells, a stem cell-containing population that has been shown to be highly abundant in several kinds of tissues, in PDL cells. Isolated SP cells from the rat PDL exhibited a superior ability to differentiate into osteoblastic cells compared with non-SP (NSP) and unsorted PDL cells in vitro. The mRNA expressions of osteoblast markers and bone morphogenetic protein (BMP) 2 were significantly upregulated in SP cells and were further increased by osteogenic induction. To examine the bone-forming activity of SP cells in vivo, PDL SP cells isolated from green fluorescent protein (GFP)-transgenic rats were transplanted with hydroxyapatite (HA) disks into wild-type animals. SP cells exhibited a high ability to induce the mineralized matrix compared with NSP and unsorted PDL cells. At 12 weeks after the implantation, some of the pores in the HA disks with SP cells were filled with mineralized matrices, which were positive for bone matrix proteins, such as osteopontin, bone sialoprotein, and osteocalcin. Furthermore, osteoblast- and osteocyte-like cells on and in the bone-like mineralized matrices were GFP positive, suggesting that the matrices were directly formed by the transplanted cells. These results suggest that PDL SP cells possess enhanced osteogenic potential and could be a potential source for cell-based regenerative therapy for alveolar bone.
authors: Division of Hard Tissue Research, Institute for Oral Science, Matsumoto Dental University, Shiojiri, Nagano, Japan.
authors: Tadashi
authors: T
authors: Ninomiya
authors: Toru
authors: T
authors: Hiraga
authors: Akihiro
authors: A
authors: Hosoya
authors: Kiyoshi
authors: K
authors: Ohnuma
authors: Yuzuru
authors: Y
authors: Ito
authors: Masafumi
authors: M
authors: Takahashi
authors: Susumu
authors: S
authors: Ito
authors: Makoto
authors: M
authors: Asashima
authors: Hiroaki
authors: H
authors: Nakamura
mesh_terms: D050156:Adipogenesis; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001861:Bone Regeneration; D001842:Bone and Bones / Q000473:pathology; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D010012:Osteogenesis / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology*; D051381:Rats; D055647:Rats, Transgenic; D058985:Side-Population Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D015854:Up-Regulation
chemical_list: D055396:Bone Morphogenetic Protein 2; D049452:Green Fluorescent Proteins