title: Hes3 regulates cell number in cultures from glioblastoma multiforme with stem cell characteristics.
abstract: Tumors exhibit complex organization and contain a variety of cell populations. The realization that the regenerative properties of a tumor may be largely confined to a cell subpopulation (cancer stem cell) is driving a new era of anti-cancer research. Cancer stem cells from Glioblastoma Multiforme tumors express markers that are also expressed in non-cancerous neural stem cells, including nestin and Sox2. We previously showed that the transcription factor Hes3 is a marker of neural stem cells, and that its expression is inhibited by JAK activity. Here we show that Hes3 is also expressed in cultures from glioblastoma multiforme which express neural stem cell markers, can differentiate into neurons and glia, and can recapitulate the tumor of origin when transplanted into immunocompromised mice. Similar to observations in neural stem cells, JAK inhibits Hes3 expression. Hes3 RNA interference reduces the number of cultured glioblastoma cells suggesting a novel therapeutic strategy.
authors: Department of Neurological Surgery, University of Virginia, Virginia, USA.
authors: Deric M
authors: DM
authors: Park
authors: Jinkyu
authors: J
authors: Jung
authors: Jimmy
authors: J
authors: Masjkur
authors: Stylianos
authors: S
authors: Makrogkikas
authors: Doreen
authors: D
authors: Ebermann
authors: Sarama
authors: S
authors: Saha
authors: Roberta
authors: R
authors: Rogliano
authors: Nicoletta
authors: N
authors: Paolillo
authors: Simone
authors: S
authors: Pacioni
authors: Ron D
authors: RD
authors: McKay
authors: Steve
authors: S
authors: Poser
authors: Andreas
authors: A
authors: Androutsellis-Theotokis
mesh_terms: D042702:Angiopoietin-2 / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016543:Central Nervous System Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D053613:Janus Kinase 1 / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D050796:STAT3 Transcription Factor / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D042702:Angiopoietin-2; D015415:Biomarkers; D004268:DNA-Binding Proteins; C495457:HES3 protein, human; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D053613:Janus Kinase 1
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