title: Production of amino-terminally acetylated recombinant proteins in E. coli.
abstract: The majority of proteins in eukaryote cells are subjected to amino-terminal acetylation. This co-translational modification can affect the stability of a protein and also regulate its biological function. Amino-terminally acetylated recombinant proteins cannot be produced using prokaryote expression systems, such as E. coli, as these cells lack the appropriate N-α-terminal acetyltransferase complexes. Here we describe a simple protocol that allows the recombinant expression and purification of NatB-dependent amino-terminally acetylated proteins from E. coli.
authors: School of Biosciences, University of Kent, Canterbury, UK.
authors: Matthew
authors: M
authors: Johnson
authors: Michael A
authors: MA
authors: Geeves
authors: Daniel P
authors: DP
authors: Mulvihill
mesh_terms: D000107:Acetylation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D063206:N-Terminal Acetyltransferases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D014335:Tropomyosin / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014335:Tropomyosin; D063206:N-Terminal Acetyltransferases
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