title: Storage of oral fluid as dried spots on alginate and chitosan foam - a new concept for oral fluid collection.
abstract: BACKGROUND
  In recent years, there has been a major focus on analyzing drug substances from dried µl volumes of biological fluids, commonly known as dried matrix spotting.
  
  
  RESULTS
  10 µl oral fluid, spiked with five basic drugs, was collected and stored as dried spots on alginate and chitosan foam, and subsequently dissolved; the drugs were thereafter isolated with electromembrane extraction and analyzed by LC-MS. The correlation coefficients were above 0.9885 for all drugs in the range 25-1000 ng/ml. The reported RSD values were below 15%.
  
  
  CONCLUSIONS
  Storage of oral fluid as dried spots on alginate and chitosan foam have been investigated for the first time. The extract obtained after electromembrane extraction was directly compatible with LC-MS, and apparently free from coextracted matrix components.
authors: School of Pharmacy, University of Oslo, PO Box 1068 Blindern, 0316 Oslo, Norway.
authors: Christine Pernille
authors: CP
authors: Lødøen
authors: Lars Erik
authors: LE
authors: Eng Eibak
authors: Knut Einar
authors: KE
authors: Rasmussen
authors: Stig
authors: S
authors: Pedersen-Bjergaard
authors: Therese
authors: T
authors: Andersen
authors: Astrid
authors: A
authors: Gjelstad
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D004364:Pharmaceutical Preparations; D020723:Glucuronic Acid; D048271:Chitosan