title: Comparative analysis of the biological and physical properties of Enterococcus faecalis bacteriophage vB_EfaS_GEC-EfS_3 and Streptococcus mitis bacteriophage vB_SmM_GEC-SmitisM_2.
abstract: Enterococcus faecalis and Streptococcus mitis are common commensal inhabitants of the human gastrointestinal and genitourinary tracts. However, both species can be opportunistic pathogens and cause disease in nosocomial settings. These infections can be difficult to treat because of the frequency of antibiotic resistance among these strains. Bacteriophages are often suggested as an alternative therapeutic agent against these infections. In this study, E. faecalis and S. mitis strains were isolated from female patients with urinary tract infections. Bacteriophages active against these strains were isolated from sewage water from the Mtkvari River. Two phages, designated vB_EfaS_GEC-EfS_3 (Syphoviridae) and vB_SmM_GEC-SmitisM_2 (Myoviridae), were specific for E. faecalis and S. mitis, respectively. Each phage's growth patterns and adsorption rates were quantified. Sensitivity to ultraviolet light and temperature was determined, as was host range and serology. The S. mitis bacteriophage was found to be more resistant to ultraviolet light and exposure to high temperatures than the E. faecalis bacteriophage, despite having a much greater rate of replication. While each phage was able to infect a broad range of strains of the same species as the host species from which they were isolated, they were unable to infect other host species tested.
authors: Ilia State University, Tbilisi, GA, USA.
authors: Sophio
authors: S
authors: Rigvava
authors: Irina
authors: I
authors: Tchgkonia
authors: Darejan
authors: D
authors: Jgenti
authors: Teona
authors: T
authors: Dvalidze
authors: James
authors: J
authors: Carpino
authors: Marina
authors: M
authors: Goderdzishvili
mesh_terms: D001435:Bacteriophages / Q000302:isolation & purification / Q000502:physiology* / Q000528:radiation effects / Q000648:ultrastructure; D013293:Enterococcus faecalis / Q000821:virology*; D005260:Female; D058507:Host Specificity; D006801:Humans; D046529:Microscopy, Electron, Transmission; D012703:Serotyping; D017108:Streptococcus Phages / Q000502:physiology* / Q000528:radiation effects / Q000648:ultrastructure; D034361:Streptococcus mitis / Q000821:virology*; D013696:Temperature; D014466:Ultraviolet Rays