title: Utility of OCT3/4, TSPY and β-catenin as biological markers for gonadoblastoma formation and malignant germ cell tumor development in dysgenetic gonads.
abstract: BACKGROUND
  Gonadoblastoma (GB) is regarded as an in situ form of germ cell tumor in dysgenetic gonads, and 30% of patients with GB develop a dysgerminoma/seminoma tumor.
  
  
  OBJECTIVE
  Determine whether OCT3/4 and β-catenin are expressed in dysgenetic gonads before GB development and whether TSPY participates in the OCT3/4-β-catenin pathways in the malignant invasive behavior.
  
  
  METHODS
  dysgenetic gonads of Disorders of sex differentiation (DSD) patients with mixed gonadal dysgenesis were analyzed by immunohistochemistry and immunofluorescence for comparison with GB and dysgerminoma/seminoma.
  
  
  RESULTS
  Our results suggest that the development of GB is secondary to the interaction of OCT3/4 and TSPY, that β-catenin does not participate in this process.
  
  
  CONCLUSIONS
  The use of this biological markers detects the potential high risk gonads.
authors: Molecular and Cellular Morphology Laboratory, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico.
authors: Icela
authors: I
authors: Palma
authors: Nayely
authors: N
authors: Garibay
authors: Rocio
authors: R
authors: Pena-Yolanda
authors: Alejandra
authors: A
authors: Contreras
authors: Atlantida
authors: A
authors: Raya
authors: Carolina
authors: C
authors: Dominguez
authors: Mirna
authors: M
authors: Romero
authors: Gerardo
authors: G
authors: Aristi
authors: Gloria
authors: G
authors: Queipo
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000032:analysis*; D002648:Child; D004407:Dysgerminoma / Q000175:diagnosis; D006059:Gonadal Dysgenesis / Q000175:diagnosis*; D018238:Gonadoblastoma / Q000175:diagnosis*; D006801:Humans; D018309:Neoplasms, Gonadal Tissue / Q000175:diagnosis*; D050814:Octamer Transcription Factor-3 / Q000032:analysis*; D051176:beta Catenin / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; C495270:CTNNB1 protein, human; D018797:Cell Cycle Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C497749:TSPY1 protein, human; D051176:beta Catenin