title: Epidermal growth factor promotes proliferation and improves restoration after intestinal ischemia-reperfusion injury in rats.
abstract: Epidermal growth factor (EGF) is an attractive and promising therapeutic application for intestinal disorders. The current study examined its influence on proliferation and restoration after ischemia-reperfusion (I/R) injury in rat small intestine. Six groups were performed: sham operation (Con); ischemia for 30 min with subsequent reperfusion for 30 min (I/R); I/R injured with 500 μg/kg EGF injected 5 min before ischemia (Pre-l); I/R injured with 50 μg/kg EGF injected 5 min before ischemia (Pre-s); I/R injured with 500 μg/kg EGF injected 5 min after reperfusion (Post-l); and I/R injured with 50 μg/kg EGF injected 5 min after reperfusion (Post-s). Intestinal histological damage, crypt cell proliferation degree, mucosal permeability, tight junction proteins expression, and levels of inflammation factors were studied for each group. Compared with the I/R group, administration of EGF in the Pre-l, Pre-s, and Post-l groups all presented a significant proliferation effect. The levels of FD4, IL-6, and TNF-α were dramatically decreased in all EGF-treated groups. Histological destruction was improved and TJs recovery was notably accelerated in all EGF-treated groups except the Post-s group. D-lactate concentration was only diminished in the Pre-l group. These results suggest that mucosally applied EGF can promote intestinal proliferation and improve restoration after I/R injury. EGF intraluminal administration is an effective treatment against intestinal I/R injury.
authors: Department of Intensive Care Unit, Jiangsu Province Chinese Medicine Hospital, 155 Han Zhong Road, Nanjing 210029, China.
authors: Yanxia
authors: Y
authors: Geng
authors: Jieshou
authors: J
authors: Li
authors: Fan
authors: F
authors: Wang
authors: Qiurong
authors: Q
authors: Li
authors: Xing
authors: X
authors: Wang
authors: Liqun
authors: L
authors: Sun
authors: Weiqin
authors: W
authors: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D007249:Inflammation / Q000188:drug therapy*; D015850:Interleukin-6 / Q000097:blood; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D062725:Tight Junction Proteins / Q000096:biosynthesis; D019108:Tight Junctions / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D062725:Tight Junction Proteins; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor
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