title: Immunological response in H22 transplanted mice undergoing Aconitum coreanum polysaccharide treatment.
abstract: In this study, a polysaccharide (ACP-a1), with a molecular weight of 3.2×10(5)Da, was successfully purified and identified from the roots of Aconitum coreanum (Lèvl.) Rapaics. Gas chromatography (GC) analysis indicated that ACP-a1 was mainly composed of β-d-mannose and β-d-glucose in a molar ratio of 1.2:3.5. The effects of ACP-a1 on the tumor growth and immune function were assessed in hepatoma H22 bearing mice. Results showed that ACP-a1 significantly inhibited the growth of hepatoma H22 transplanted in mice and prolonged the survival time of H22 tumor-bearing mice. Besides, the body weight, peripheral white blood cells (WBC), thymus index and spleen index of H22 tumor-bearing were also improved after ACP-a1 treatment. Furthermore, ACP-a1 could promote the secretion of serum cytokines in H22 tumor-bearing mice, such as IL-2, TNF-α and IFN-γ. Taken together, our results indicate that ACP-a1 inhibits tumor growth in vivo at least partly via improving immune responses of host organism, and seems to be safe and effective as a novel agent with immunomodulatory activity for the use of anti-tumor therapy.
authors: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, PR China.
authors: Huisheng
authors: H
authors: Li
authors: Meina
authors: M
authors: Sun
authors: Jin
authors: J
authors: Xu
authors: Hui
authors: H
authors: Li
authors: Meng
authors: M
authors: Zang
authors: Yunfu
authors: Y
authors: Cui
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000276:immunology / Q000401:mortality; D045744:Cell Line, Tumor; D016207:Cytokines / Q000097:blood / Q000276:immunology; D005260:Female; D007958:Leukocyte Count; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000276:immunology / Q000401:mortality; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016207:Cytokines; D010936:Plant Extracts; D011134:Polysaccharides