title: Ultrafast cell switching for recording cell surface transitions: new insights into epidermal growth factor receptor signalling.
abstract: A pinched-flow deflection technology was developed for rapid single cell switching between biochemical microenvironments. Millisecond switching was used to stimulate and preserve epidermal growth factor receptor (EGFR) autophosphorylation transitions. Intramolecular phosphorylation initiates signal transduction, is silenced by phosphatase activity until EGFR dimerization enables intermolecular phosphorylation to initiate downstream signalling.
authors: Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., 44227 Dortmund, Germany. yychiang@mems.iam.ntu.edu.tw
authors: Ya-Yu
authors: YY
authors: Chiang
authors: Jonathan
authors: J
authors: West
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D019281:Dimerization; D066246:ErbB Receptors / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D066246:ErbB Receptors