title: Oxidative stress markers in laparoscopic versus open colectomy for cancer: a double-blind randomized study.
abstract: BACKGROUND
  Colorectal cancer as well as colorectal surgery is associated with increased oxidative stress through different mechanisms. In this study the levels of different oxidative stress markers were comparatively assessed in patients who underwent laparoscopic or conventional resection for colorectal cancer.
  
  
  METHODS
  Sixty patients with colorectal cancer were randomly assigned to undergo laparoscopic (LS) or open surgery (OS). Lipid, protein, RNA, and nitrogen damage was investigated by measuring serum 8-isoprostanes (8-epiPGF2α), protein carbonyls (PC), 8-hydroxyguanosine (8-OHG), and 3-nitrotyrosine (3-NT), respectively. The primary end point of the study was to analyze and compare serum levels of the oxidative stress markers between the groups.
  
  
  RESULTS
  Postoperative serum levels of 8-epiPGF2α, 3-NT, and 8-OHG were significantly lower in the LS group at 24 h after surgery (p < 0.05). At 6 h postoperatively, the levels of 8-epiPGF2α and 3-NT were significantly lower in the LS group (p < 0.05). No difference in the levels of PC was found between the two groups at any time point. In the OS group, postoperative levels of 8-epiPGF2α were significantly lower than the preoperative values (p < 0.01). In the LS group, the postoperative values of 8-epiPGF2α, 3-NT, and 8-OHG were significantly lower than the preoperative values (p < 0.05).
  
  
  CONCLUSIONS
  Laparoscopic surgery for colorectal cancer is associated with lower oxidative stress compared to open surgery. 8-epiPGF2α was the most suitable marker for readily defining the oxidative status in patients who underwent surgery for colorectal cancer.
authors: Department of General Surgery, "G. Hatzikosta" General Hospital, Ioannina, Greece. atlscrete@med.uoc.gr
authors: George
authors: G
authors: Pappas-Gogos
authors: Constantinos
authors: C
authors: Tellis
authors: Konstantinos
authors: K
authors: Lasithiotakis
authors: Alexandros D
authors: AD
authors: Tselepis
authors: Konstantinos
authors: K
authors: Tsimogiannis
authors: Evangelos
authors: E
authors: Tsimoyiannis
authors: George
authors: G
authors: Chalkiadakis
authors: Emmanuel
authors: E
authors: Chrysos
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D003082:Colectomy / Q000379:methods*; D015179:Colorectal Neoplasms / Q000601:surgery*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D004311:Double-Blind Method; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives / Q000097:blood; D006801:Humans; D010535:Laparoscopy; D008297:Male; D018384:Oxidative Stress; D011184:Postoperative Period; D050050:Protein Carbonylation; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D015415:Biomarkers; D006151:Guanosine; C075750:8-epi-prostaglandin F2alpha; C002744:3-nitrotyrosine; C046215:8-hydroxyguanosine; D014443:Tyrosine; D015237:Dinoprost
references: Anesth Analg. 2008 Dec;107(6):1946-52
references: 19020142
references: FASEB J. 2004 Dec;18(15):1791-800
references: 15576482
references: Surg Endosc. 2000 Jul;14(7):649-52
references: 10948302
references: IUBMB Life. 2006 Oct;58(10):581-8
references: 17050375
references: J Immunol. 2004 Feb 15;172(4):2522-9
references: 14764725
references: Cell Mol Life Sci. 2010 Jun;67(11):1817-29
references: 20148281
references: Surg Endosc. 2012 Feb;26(2):330-6
references: 21898023
references: Exp Biol Med (Maywood). 2003 May;228(5):525-8
references: 12709580
references: Br J Surg. 1994 Aug;81(8):1219-23
references: 7953368
references: Angiology. 2005 Jul-Aug;56(4):459-65
references: 16079930
references: Cancer. 1989 Jul 15;64(2):422-5
references: 2544250
references: Free Radic Biol Med. 2005 May 15;38(10):1323-32
references: 15855050
references: Br J Surg. 2006 Feb;93(2):195-204
references: 16392101
references: Toxicol Pathol. 2010 Jan;38(1):96-109
references: 20019356
references: Ann Surg. 2006 Mar;243(3):359-63
references: 16495701
references: Lancet. 2002 Jun 29;359(9325):2224-9
references: 12103285
references: IUBMB Life. 2004 Apr;56(4):185-91
references: 15230345
references: World J Surg. 2008 Feb;32(2):288-95
references: 18074171
references: Eur Surg Res. 2002 Sep-Oct;34(5):346-50
references: 12364818
references: J Surg Res. 2011 Jul;169(1):e59-68
references: 21492871
references: J Surg Res. 2000 Aug;92(2):291-300
references: 10896836
references: Eur Respir J Suppl. 2003 Sep;44:4s-6s
references: 14582891
references: J Clin Gastroenterol. 2000 Jan;30(1):47-51
references: 10636209
references: Clin Chim Acta. 2003 Mar;329(1-2):23-38
references: 12589963
references: World J Surg. 1996 Jan;20(1):11-6
references: 8588401
references: Carcinogenesis. 2010 Feb;31(2):228-33
references: 19933708
references: Hum Reprod. 2003 Oct;18(10):2181-8
references: 14507842
references: Biomed Environ Sci. 2008 Aug;21(4):286-9
references: 18837290
references: Ann Surg. 2009 Jul;250(1):54-61
references: 19561481
references: J Am Assoc Gynecol Laparosc. 2003 Feb;10(1):65-74
references: 12554997
references: J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009 Apr;27(2):120-39
references: 19412858
references: Br J Surg. 2007 Mar;94(3):362-8
references: 17143850
references: Surg Endosc. 2008 May;22(5):1146-60
references: 18071810
references: Anesthesiology. 2001 Jun;94(6):1133-8
references: 11465607
references: Free Radic Biol Med. 2010 Dec 1;49(11):1603-16
references: 20840865
references: Biochem Pharmacol. 2006 Nov 30;72(11):1493-505
references: 16723122
references: Br J Anaesth. 2001 Nov;87(5):774-7
references: 11878531
references: Int J Cancer. 2002 Oct 1;101(4):395-7
references: 12209966
references: Am J Pathol. 2003 Mar;162(3):793-801
references: 12598314
references: World J Surg. 2011 Aug;35(8):1911-7
references: 21567262
references: Ann Surg. 2002 Dec;236(6):759-66; disscussion 767
references: 12454514
references: BMB Rep. 2008 Mar 31;41(3):194-203
references: 18377722