title: Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice.
abstract: The major quantitative trait locus qBR9.1 confers broad-spectrum resistance to rice blast, and was mapped to a ~69.1 kb region on chromosome 9 that was inherited from resistant variety Sanhuangzhan No 2 (SHZ-2). Within this region, only one predicted disease resistance gene with nucleotide binding site and leucine-rich repeat (NBS-LRR) domains was found. Specific markers corresponding to this gene cosegregated with blast resistance in F2 and F3 populations derived from crosses of susceptible variety Texianzhan 13 (TXZ-13) to SHZ-2 and the resistant backcross line BC-10. We tentatively designate the gene as Pi56(t). Sequence analysis revealed that Pi56(t) encodes an NBS-LRR protein composed of 743 amino acids. Pi56(t) was highly induced by blast infection in resistant lines SHZ-2 and BC-10. The corresponding allele of Pi56(t) in the susceptible line TXZ-13 encodes a protein with an NBS domain but without LRR domain, and it was not induced by Magnaporthe oryzae infection. Three new cosegregating gene-specific markers, CRG4-1, CRG4-2 and CRG4-3, were developed. In addition, we evaluated polymorphism of the gene-based markers among popular varieties from national breeding programs in Asia and Africa. The presence of the CRG4-2 SHZ-2 allele cosegregated with a blast-resistant phenotype in two BC2F1 families of SHZ-2 crossed to recurrent parents IR64-Sub1 and Swarna-Sub1. CRG4-1 and CRG4-3 showed clear polymorphism among 19 varieties, suggesting that they can be used in marker-assisted breeding to combine Pi56(t) with other target genes in breeding lines.
authors: International Rice Research Institute, Plant Breeding, Genetics and Biotechnology Division, DAPO Box 7777, Metro Manila, Philippines.
authors: Yan
authors: Y
authors: Liu
authors: Bin
authors: B
authors: Liu
authors: Xiaoyuan
authors: X
authors: Zhu
authors: Jianyuan
authors: J
authors: Yang
authors: Alicia
authors: A
authors: Bordeos
authors: Guoliang
authors: G
authors: Wang
authors: Jan E
authors: JE
authors: Leach
authors: Hei
authors: H
authors: Leung
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001947:Breeding / Q000379:methods; D002681:China; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D056726:Genetic Association Studies; D005819:Genetic Markers / Q000235:genetics*; D020082:Magnaporthe; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010935:Plant Diseases / Q000382:microbiology*; D017434:Protein Structure, Tertiary / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005819:Genetic Markers
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