title: Visible-light-mediated thiol-ene hydrogelation using eosin-Y as the only photoinitiator.
abstract: The utility of visible-light-mediated polymerization in tissue engineering has been limited due to the necessary use of potentially cytotoxic coinitiator and comonomer. Here, we report a visible-light-mediated thiol-ene hydrogelation scheme using eosin-Y as the only photoinitiator. Under visible light exposure, rapid and highly tunable step-growth gelation is achieved using PEG-norbornene and a model cross-linker dithiothreitol. In addition to investigating the gelation kinetics and properties of thiol-ene hydrogels formed by this new gelation scheme, we also report high cytocompatibility of these hydrogels using human mesenchymal stem cells (hMSCs) and pancreatic MIN6 β-cells.
authors: Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, Indianapolis, IN 46202, USA.
authors: Han
authors: H
authors: Shih
authors: Chien-Chi
authors: CC
authors: Lin
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002470:Cell Survival; D004801:Eosine Yellowish-(YS) / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D050417:Insulin-Secreting Cells / Q000166:cytology; D008027:Light; D059630:Mesenchymal Stem Cells / Q000166:cytology; D017319:Photosensitizing Agents / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization / Q000528:radiation effects; D023822:Tissue Engineering / Q000295:instrumentation*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D017319:Photosensitizing Agents; D011092:Polyethylene Glycols; D004801:Eosine Yellowish-(YS)