title: Sensitive detection of human breast cancer cells based on aptamer-cell-aptamer sandwich architecture.
abstract: Breast cancer is one of the most critical threats to the health of women, and the development of new methods for early diagnosis is urgently required, so this paper reports a method to detect Michigan cancer foundation-7 (MCF-7) human breast cancer cells with considerable sensitivity and selectivity by using electrochemical technique. In this method, a mucin 1 (MUC1)-binding aptamer is adopted to recognize MCF-7 human breast cancer cells, while enzyme labeling is employed to produce amplified catalytic signals. The molecular recognition and the signal amplification are elaborately integrated by fabricating an aptamer-cell-aptamer sandwich architecture on an electrode surface, thus a biosensor for the detection of MCF-7 is fabricated based on the architecture. The detection range can be from 100 to 1×10(7) cells, and the detection limit can be as low as 100 cells. The method is also cost-effective and conveniently operated, implying potential help for the development of early diagnosis of breast cancer.
authors: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, PR China.
authors: Xiaoli
authors: X
authors: Zhu
authors: Jinghua
authors: J
authors: Yang
authors: Min
authors: M
authors: Liu
authors: Yao
authors: Y
authors: Wu
authors: Zhongming
authors: Z
authors: Shen
authors: Genxi
authors: G
authors: Li
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000175:diagnosis; D058266:Dielectric Spectroscopy; D055088:Early Detection of Cancer; D055664:Electrochemical Techniques; D004566:Electrodes; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D018396:Mucin-1 / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D018396:Mucin-1; D006735:Horseradish Peroxidase