title: Selection of aptamers against inactive Vibrio alginolyticus and application in a qualitative detection assay.
abstract: Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. Using these aptamers and PCR, V. alginolyticus could be detected at 100 cells/ml. Sequencing of the final pool of aptamers revealed that some sequences, termed high-frequency aptamers, appeared more than once; these may be of practical application. All sequences obtained were divided into nine families according to their homology tree, some conserved sequences were also found in each of the six families. One sequence was found in significant proportions of the aptamers, suggesting that this conserved sequence might be important for forming the three-dimensional aptamer structure.
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College of Jimei University, No. 43, Yindou Road, Xiamen, 361021, Fujian, China. inming@126.com
authors: Xuemin
authors: X
authors: Tang
authors: Jiang
authors: J
authors: Zheng
authors: Qinpi
authors: Q
authors: Yan
authors: Zhongbao
authors: Z
authors: Li
authors: Yubao
authors: Y
authors: Li
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D052156:SELEX Aptamer Technique; D017422:Sequence Analysis, DNA; D014735:Vibrio Infections / Q000175:diagnosis / Q000382:microbiology; D044149:Vibrio alginolyticus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D052157:Aptamers, Nucleotide