title: Stabilization of mitochondrial and microsomal function of fucoidan from Sargassum plagiophyllum in diethylnitrosamine induced hepatocarcinogenesis.
abstract: Crude fucoidan from Sargassum plagiophyllum extracted from blade and purified by Q-Sepharose fast flow anion-exchange chromatography and three fucoidan fractions were obtained. Maximum sulphate containing fucoidan fraction was considered as purified fucoidan and purity was checked with agarose gel electrophoresis. The monosaccharides of purified fucoidan analysed by HPLC revealed the presence of the sugars such as fucose as a major sugar were 70.8 mol%. The percentages of other sugars were galactose (13.5%), xylose (2.5%) and mannose (11.2%). GPC was used to analyse molecular weight of purified fucoidan and it was found to be 35 kDa. The levels of ICDH, SDH, MDH, a-KGDH, Phase-I biotransformation enzymes, and Phase-II biotransformation enzymes were decreased in cancer bearing animals which may be due to oxidative stress and mitochondrial damage and fucoidan restored these enzyme activities. The inhibition of carcinogen metabolic activation indicates the anticancer activity of fucoidan in DEN induced liver cancer.
authors: Department of Botany, Annamalai University, Annamalai Nagar, TN, India. veeraperumalsuresh@gmail.com
authors: Veeraperumal
authors: V
authors: Suresh
authors: Chinnathambi
authors: C
authors: Anbazhagan
authors: Ramar
authors: R
authors: Thangam
authors: Dharmaraj
authors: D
authors: Senthilkumar
authors: Namasivayam
authors: N
authors: Senthilkumar
authors: Soundarapandian
authors: S
authors: Kannan
authors: Ramasamy
authors: R
authors: Rengasamy
authors: Perumal
authors: P
authors: Palani
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001711:Biotransformation / Q000187:drug effects; D002952:Citric Acid Cycle / Q000187:drug effects; D004052:Diethylnitrosamine / Q000633:toxicity*; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D044647:Sargassum / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D004052:Diethylnitrosamine; C007789:fucoidan