title: Phylogenetic analysis of isolated HCV strains from tunisian hemodialysis patients.
abstract: The present study describes the strains of hepatitis C virus (HCV) isolated from Tunisian hemodialysis patients. Thirty-three HCV strains isolated from different dialysis centers in Tunis City were amplified by RT-PCR in a region of the NS5b gene, genotyped by sequencing, and compared to international sequences by phylogenetic analysis. The phylogenetic tree showed that 16 HCV isolates have been identified as subtype 4k (48.5%), 7 as unspecified HCV-4 subtype (21.2%), 5 as subtype 4a et 1b (each 15.2%). The analysis of this tree revealed that the HCV-1b strains were closely related to Anglo-Saxon and European isolates, while the HCV-4 isolates are genetically similar to Egyptian and African strains. Phylogenic analysis of 33 Tunisian isolates with international HCV strains on a region of the NS5b gene demonstrated that the subtype 4k submerged the Tunis city and a new subtype of HCV4 seems to be suspect in this area.
authors: Immunology Research Laboratory of Kidney Transplantation and Immunopathology, Charles Nicolle Hospital, Tunis El Manar University, Boulevard 9 Avril, Tunis, Tunisia.
authors: Fatma Houissa
authors: FH
authors: Kchouk
authors: Yousr
authors: Y
authors: Gorgi
authors: Lamjed
authors: L
authors: Bouslama
authors: Imen
authors: I
authors: Sfar
authors: Rym
authors: R
authors: Ayari
authors: Hacene
authors: H
authors: Khiri
authors: Phillipe
authors: P
authors: Halfon
authors: Houda
authors: H
authors: Aouadi
authors: Saloua
authors: S
authors: Jendoubi Ayed
authors: Khaled
authors: K
authors: Ayed
authors: Taieb
authors: T
authors: Ben Abdallah
mesh_terms: D000328:Adult; D016000:Cluster Analysis; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D006435:Renal Dialysis / Q000009:adverse effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014416:Tunisia / Q000453:epidemiology; D017361:Viral Nonstructural Proteins; D012324:RNA-Dependent RNA Polymerase
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase
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