title: Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides.
abstract: Identification and quantification of immunogenic peptides and tumor-derived epitopes presented on MHC-I molecules are essential for basic studies and vaccines generation. Although lymphocytes derived from transgenic mice can serve as sensitive detectors of processes of antigen presentation and recognition, they are not always available. The use of cell lines might be extremely useful. In this study, we generated a lacZ inducible CD8⁺ hybridoma (BUSA14) capable of recognizing both human and mouse gp100₂₅₋₃₃ melanoma antigens presented on dendritic and tumor cell lines. This hybridoma expresses a variety of membranal T cell markers and secretes IL-2 and TNFα. Thus, BUSA14 offers a quantifiable, cheap and straightforward tool for studying peptide presentation by MHC-I molecules on the cell surface.
authors: Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
authors: Gal
authors: G
authors: Cafri
authors: Adi
authors: A
authors: Sharbi-Yunger
authors: Esther
authors: E
authors: Tzehoval
authors: Lea
authors: L
authors: Eisenbach
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000378:metabolism; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism; D007763:Lac Operon; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D058951:gp100 Melanoma Antigen / Q000378:metabolism*
chemical_list: D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D058951:gp100 Melanoma Antigen
references: J Exp Med. 1987 Feb 1;165(2):395-407
references: 3102668
references: Cancer Res. 1995 Nov 1;55(21):4941-9
references: 7585534
references: J Immunol Methods. 1992 May 18;149(2):255-60
references: 1593137
references: J Immunol. 1997 Mar 15;158(6):2723-30
references: 9058806
references: Nat Med. 1995 Jul;1(7):649-53
references: 7585145
references: Int Immunol. 1994 Mar;6(3):369-76
references: 8186188
references: Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6020-4
references: 1378619
references: Proc Natl Acad Sci U S A. 1991 May 1;88(9):3972-6
references: 1902576
references: J Exp Med. 1998 Jul 20;188(2):277-86
references: 9670040
references: Science. 1988 Jul 8;241(4862):202-5
references: 3260404
references: Methods Mol Biol. 2001;156:265-72
references: 11068767