title: FADS gene polymorphisms confer the risk of coronary artery disease in a Chinese Han population through the altered desaturase activities: based on high-resolution melting analysis.
abstract: OBJECTIVE
  We explored the desaturase activities and the correlation of fatty acid desaturases (FADS) gene single nucleotide polymorphisms (SNPs) with plasma fatty acid in coronary artery disease (CAD) patients in a Chinese Han population.
  
  
  METHODS
  Plasma fatty acids were measured by gas chromatography in CAD patients (n = 505) and a control group (n = 510). Five SNPs in the FADS gene were genotyped with high-resolution melting (HRM) methods.
  
  
  RESULTS
  After adjustment, D6D activity, assessed as arachidonic acid (AA, C20:4n-6)/linoleic acid (LA, C18:2n-6), was higher in CAD patients (p<0.001). D9D activity, which was estimated as the ratio of palmitoleic acid (C16:1)/palmitic acid (C16:0) or oleic acid (C18:1n-9) to stearic acid (C18:0), was also increased (p<0.001). The genotype distributions of rs174537 G>T and rs174460 C>T were different between the two groups. The rs174537 T allele was associated with a lower risk of CAD [OR 0.743, 95% CI (0.624, 0.884), p = 0.001]. Carriers of the rs174460 C allele were associated with a higher risk of CAD [OR 1.357, 95% CI (1.106, 1.665), p = 0.003].
  
  
  CONCLUSIONS
  We firstly report that the rs174460 C allele is associated with a higher risk of CAD, and confirm that the rs174537 T allele is associated with a lower risk of CAD. Our results indicate that FADS gene polymorphisms are likely to influence plasma fatty acid concentrations and desaturase activities.
authors: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China.
authors: Si-Wei
authors: SW
authors: Li
authors: Kun
authors: K
authors: Lin
authors: Pei
authors: P
authors: Ma
authors: Zhen-Lu
authors: ZL
authors: Zhang
authors: Yi-Dan
authors: YD
authors: Zhou
authors: Shuang-Yan
authors: SY
authors: Lu
authors: Xin
authors: X
authors: Zhou
authors: Song-Mei
authors: SM
authors: Liu
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D003324:Coronary Artery Disease / Q000235:genetics*; D044943:Fatty Acid Desaturases / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D005810:Multigene Family / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D005227:Fatty Acids; D044943:Fatty Acid Desaturases
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