title: Melittin derived peptides for nanoparticle based siRNA transfection.
abstract: Traditional transfection agents including cationic lipids and polymers have high efficiency but cause cytotoxicity. While cell penetrating peptide based transfection agents exhibit improved cytotoxicity profiles, they do not have the efficiency of existing lipidic agents due to endosomal trapping. As a consequence, we propose an alternative method to efficient peptide based siRNA transfection by starting with melittin, a known pore-forming peptide. By incorporating modifications to decrease cytotoxicity and improve siRNA binding, we have developed p5RHH, which can complex siRNA to form nanoparticles of 190 nm in diameter. p5RHH exhibits high efficiency with GFP knockdown at concentrations as low as 5 nM, with negligible cytotoxicity. To date, p5RHH has shown the ability to transfect B16 cells, Human Umbilical Vein Endothelial Cells, and RAW264.7 cells with high efficiency. These in vitro models demonstrate that p5RHH mediated transfection can block cancer cell proliferation, angiogenesis, and foam cell formation. Moreover, p5RHH/siRNA nanoparticles maintain their size and transfection efficiency in the presence of serum proteins suggesting the potential for use of p5RHH in vivo. These data suggest that our strategy for development of siRNA transfecting peptides can provide an avenue to safe and effective siRNA therapeutics.
authors: Computational and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63108, USA.
authors: Kirk K
authors: KK
authors: Hou
authors: Hua
authors: H
authors: Pan
authors: Gregory M
authors: GM
authors: Lanza
authors: Samuel A
authors: SA
authors: Wickline
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D008555:Melitten / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D010455:Peptides; D034741:RNA, Small Interfering; D008555:Melitten
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