title: Genetic diversity of Plasmodium falciparum field isolates from south western Nigeria.
abstract: BACKGROUND
  Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria.
  
  
  METHODS
  In this study, 100 P. falciparum strains merozoite surface protein 1(msp-1), merozoite surface protein 2 (msp-2) and Glutamate rich protein (Glurp) from Ogun State General Hospitals were characterized. The genetic diversity of P. falciparum isolates was analyzed by restriction fragment length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) of their respective allelic families KI, MAD 20, RO33 (MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively.
  
  
  RESULTS
  Majority of the patients showed monoclonal infections while multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2 respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3 MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for Glurp with allelic size ranged between 700 and 900 bp.
  
  
  CONCLUSIONS
  The allelic distributions however were similar to those previously reported in other endemic malaria countries. Future studies will be designed to include other malaria endemic regions of Nigeria such as the oil exploration regions.
authors: Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Nigeria.
authors: G I
authors: GI
authors: Olasehinde
authors: C S
authors: CS
authors: Yah
authors: R
authors: R
authors: Singh
authors: O Olusola
authors: OO
authors: Ojuronbge
authors: A A
authors: AA
authors: Ajayi
authors: N
authors: N
authors: Valecha
authors: A O
authors: AO
authors: Abolaji
authors: A O
authors: AO
authors: Adeyeba
mesh_terms: D000293:Adolescent; D000328:Adult; D000953:Antigens, Protozoan / Q000235:genetics*; D016054:DNA, Protozoan; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000235:genetics / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D008875:Middle Aged; D009549:Nigeria / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium; D018698:Glutamic Acid
keywords: Genetic diversity; Merozoite surface protein; Plasmodium; monoclonal infection
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