title: Cut-and-Paste of DNA Using an Artificial Restriction  DNA Cutter.
abstract: DNA manipulations using a completely chemistry-based DNA cutter (ARCUT) have been reviewed. This cutter, recently developed by the authors, is composed of Ce(IV)/EDTA complex and two strands of pseudo-complementary peptide nucleic acid. The site-selective scission proceeds via hydrolysis of targeted phosphodiester linkages, so that the resultant scission fragments can be easily ligated with other fragments by using DNA ligase. Importantly, scission-site and site-specificity of the cutter are freely tuned in terms of the Watson-Crick rule. Thus, when one should like to manipulate DNA according to the need, he or she does not have to think about (1) whether appropriate "restriction enzyme sites" exist near the manipulation site and (2) whether the site-specificity of the restriction enzymes, if any, are sufficient to cut only the aimed position without chopping the DNA at non-targeted sites. Even the human genome can be manipulated, since ARCUT can cut the genome at only one predetermined site. Furthermore, the cutter is useful to promote homologous recombination in human cells, converting a site to desired sequence. The ARCUT-based DNA manipulation should be promising for versatile applications.
authors: Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan. komiyama@tara.tsukuba.ac.jp.
authors: Makoto
authors: M
authors: Komiyama
references: Nucleic Acids Res. 1997 Nov 15;25(22):4639-43
references: 9358176
references: Nucleic Acids Res. 2007;35(7):e53
references: 17376805
references: Nat Rev Genet. 2010 Sep;11(9):636-46
references: 20717154
references: Nat Biotechnol. 2011 Feb;29(2):143-8
references: 21179091
references: Nucleic Acids Res. 2004 Nov 01;32(19):e153
references: 15520462
references: J Am Chem Soc. 2004 Aug 25;126(33):10285-91
references: 15315441
references: J Am Chem Soc. 2009 Feb 25;131(7):2657-62
references: 19199631
references: Chem Commun (Camb). 2009 Nov 21;(43):6545-7
references: 19865644
references: Chem Commun (Camb). 2009 Nov 21;(43):6542-4
references: 19865643
references: Chembiochem. 2006 Apr;7(4):673-7
references: 16491499
references: Nat Protoc. 2008;3(4):646-54
references: 18388947
references: Curr Gene Ther. 2011 Feb;11(1):38-45
references: 21182465
references: Chembiochem. 2008 Sep 1;9(13):2120-6
references: 18688827
references: Nucleic Acids Res. 2012 Jun;40(11):e81
references: 22362741
references: Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11804-8
references: 10518531
references: Chembiochem. 2009 May 25;10(8):1279-88
references: 19396851
references: Dalton Trans. 2009 Jan 14;(2):227-39
references: 19089001
references: Nat Protoc. 2008;3(4):655-62
references: 18388948
references: Chem Soc Rev. 2011 Dec;40(12):5657-68
references: 21566825
references: Molecules. 2011 Dec 30;17(1):328-40
references: 22210171