title: First in vitro and in vivo results of an anti-human CD133-antibody coated coronary stent in the porcine model.
abstract: BACKGROUND
  Drug-eluting stents successfully reduce restenosis at the cost of delayed re-endothelialization. A novel concept to enhance re-endothelialization is the use of antibody-coated stents which capture circulating progenitor cells. A CD34-positive-cell-capturing stent was recently developed with conflicting clinical results. CD133 is a glycoprotein expressed on circulating hematopoietic and putative endothelial-regenerating cells and may be superior to CD34.
  
  
  OBJECTIVE
  The aim of our study was to develop a CD133-cell-capturing bare-metal stent and investigate feasibility, safety, and efficacy of CD133-stents in terms of re-endothelialization and neointima inhibition.
  
  
  RESULTS
  Anti-human CD133-antibodies were covalently attached to bare-metal stents. In vitro, binding capacity of CD133-stents was studied, revealing a significantly higher affinity of human CD133-positive cells to CD133-stents compared with mononuclear cells (MNCs). In vivo, 15 landrace pigs received BMS and CD133-stents in either RCX or LAD (n = 30 stents). Re-endothelialization was examined on day 1 (n = 4), 3 (n = 4) and day 7 (n = 4) using scanning electron microscopy. In histology, injury and inflammatory scores, as well as diameter restenosis were evaluated after day 7 (n = 3), 14 (n = 4), and 28 (n = 2). Overall no reduction in re-endothelialization, diameter stenosis or inflammatory score was seen with CD133-stents.
  
  
  CONCLUSIONS
  Stent coating with anti-human CD133-antibodies was successfully achieved with effective binding of CD133-positive cells. However, in vivo, no difference in re-endothelialization or neointima formation was evident with the use of CD133-stents compared with BMS. The low number of circulating CD133-positive cells and an increase in unspecific binding of MNCs over time may account for the observed lack of efficacy.
authors: Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Rheinische Friedrich-Wilhelms-Universität Bonn, Germany.
authors: Alexander
authors: A
authors: Sedaghat
authors: Jan-Malte
authors: JM
authors: Sinning
authors: Kathrin
authors: K
authors: Paul
authors: Gregor
authors: G
authors: Kirfel
authors: Georg
authors: G
authors: Nickenig
authors: Nikos
authors: N
authors: Werner
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D023903:Coronary Restenosis / Q000517:prevention & control; D023921:Coronary Stenosis; D042783:Endothelial Cells / Q000378:metabolism; D005240:Feasibility Studies; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D058426:Neointima / Q000517:prevention & control; D010455:Peptides / Q000276:immunology*; D015607:Stents; D013552:Swine; D013997:Time Factors
chemical_list: D000071916:AC133 Antigen; D000906:Antibodies; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides
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