title: Development of EGFP/GLUC-tagged Sindbis-like virus XJ-160.
abstract: Based on an infectious clone of Sindbis-like virus XJ-160, recombinant vectors containing a reporter gene (enhanced green fluorescence protein [EGFP] or Gaussia luciferase [GLUC]) were constructed by placing the reporter gene cassette containing the subgenomic promoter behind the 3' terminus of the viral structural protein gene. EGFP/GLUC-tagged Sindbis-like viruses were rescued in BHK-21 cells transfected with transcripts produced from the recombinant vectors. EGFP expression and strong luciferase activity were detected in BHK-21 cells infected with repeated passages of the EGFP/GLUC-tagged viruses, revealing the genetic stability of the chimeric viruses. The EGFP/GLUC-tagged Sindbis viruses reported will contribute to the assessment of viral replication and proliferation, tracking and elucidating Alphavirus-host interactions, and screening for antiviral compounds.
authors: State Key Laboratory for Infectious Disease Prevention and Control (SKLID), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), 100 Yingxin Street, Xuan Wu District, Beijing 100052, China.
authors: Zhu
authors: Z
authors: Wuyang
authors: Jiangjiao
authors: J
authors: Li
authors: Huanqin
authors: H
authors: Wang
authors: Ying
authors: Y
authors: He
authors: Guodong
authors: G
authors: Liang
mesh_terms: D018354:Alphavirus Infections; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D012845:Sindbis Virus / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D008156:Luciferases