title: Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria.
abstract: The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.
authors: IBBM-Instituto de Biotecnología y Biología Molecular, CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata,Calles 47 y 115 (1900), La Plata, Argentina.
authors: Mauricio J
authors: MJ
authors: Lozano
authors: María Eugenia
authors: ME
authors: Salas
authors: María de los Angeles
authors: Mde L
authors: Giusti
authors: María Carla
authors: MC
authors: Martini
authors: José Luis
authors: JL
authors: López
authors: Ileana
authors: I
authors: Salto
authors: María Florencia
authors: MF
authors: Del Papa
authors: Mariano
authors: M
authors: Pistorio
authors: Antonio
authors: A
authors: Lagares
mesh_terms: D004251:DNA Transposable Elements; D050939:Gene Fusion; D015723:Gene Library; D005827:Genetics, Microbial / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D011401:Promoter Regions, Genetic
chemical_list: D004251:DNA Transposable Elements