title: Hepatitis B virus X protein protects hepatoma and hepatic cells from complement-dependent cytotoxicity by up-regulation of CD46.
abstract: The involvement of hepatitis B virus X protein (HBx) in anti-complement-dependent cytotoxicity (CDC) activity during hepatocarcinogenesis is poorly understood. Here, we report that HBx is able to up-regulate membrane-bound complement regulatory protein CD46 in hepatoma cells and human immortalized liver cells through activating the promoter activity involving cAMP response element-binding protein (CREB)/cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2)/signal transducers and activators of transcription 3 (STAT3) signaling pathway. In contrast, the down-regulation of CD46 abolishes the resistance capability of hepatoma cells to CDC. Thus, we conclude that HBx contributes to the protection of hepatoma and hepatic cells from CDC by up-regulation of CD46.
authors: Department of Cancer Research, Key Laboratory of Molecular Microbiology and Technology of Ministry of Education, Institute for Molecular Biology and Biochemistry, College of Life Sciences, Nankai University, PR China.
authors: Shuai
authors: S
authors: Zhang
authors: Changliang
authors: C
authors: Shan
authors: Wenjing
authors: W
authors: Cui
authors: Xiaona
authors: X
authors: You
authors: Yumei
authors: Y
authors: Du
authors: Guangyao
authors: G
authors: Kong
authors: Fabao
authors: F
authors: Gao
authors: Lihong
authors: L
authors: Ye
authors: Xiaodong
authors: X
authors: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology / Q000821:virology*; D002461:Cell Line, Transformed; D003167:Complement Activation; D000242:Cyclic AMP / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000276:immunology / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology / Q000821:virology*; D051916:Membrane Cofactor Protein / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D054334:Viral Regulatory and Accessory Proteins
chemical_list: C497857:CD46 protein, human; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D051916:Membrane Cofactor Protein; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D000242:Cyclic AMP; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone