title: Polypeptide chain release factor eRF3 is a novel molecular partner of survivin.
abstract: The eukaryotic class II polypeptide chain release factor (eRF3) is an eRF1- and ribosome-dependent GTPase involved in translation termination of protein biosynthesis. eRF3 is a multifunctional protein that is also involved in chromosomal segregation and cytokinesis during mitosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family that is involved in the organisation of spindle and cell apoptosis. Interaction between survivin and eRF3a-F3 or eRF3b, encoded by the GSPT1 and GSPT2 genes, respectively, was confirmed using yeast two-hybrid (Y2H) and pull-down assays in vitro, and co-immunoprecipitation in vivo. The domains involved in the formation of the survivin-eRF3s complex have been identified. The sites on survivin that interact with eRF3 are located in the baculovirus IAP repeat domain (residues 65-76), which forms a beta-strand structure with an overall negative charge. The sites on eRF3 that interact with survivin were localised to the N-terminal domain(NTD; residues 131-200). Cell localisation experiments indicate that both factors are in the nucleus, suggesting that they cooperatively function in nuclear processes.
authors: Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, China; Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
authors: Ruilin
authors: R
authors: Xiao
authors: Yan
authors: Y
authors: Gao
authors: Quan
authors: Q
authors: Shen
authors: Cui
authors: C
authors: Li
authors: Wenjuan
authors: W
authors: Chang
authors: Baofeng
authors: B
authors: Chai
mesh_terms: D000595:Amino Acid Sequence; D006367:HeLa Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000737:chemistry / Q000378:metabolism*; D010454:Peptide Termination Factors / Q000737:chemistry / Q000502:physiology*; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D000077022:Survivin; D020798:Two-Hybrid System Techniques
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D010454:Peptide Termination Factors; D000077022:Survivin; C087971:peptide-chain-release factor 3
keywords: cellular localisation; eRF3; interaction map; survivin