title: Expression of yeast acyl-CoA-∆9 desaturase leads to accumulation of unusual monounsaturated fatty acids in soybean seeds.
abstract: An acyl-CoA-Δ9 desaturase from Saccharomyces cerevisiae was expressed by subcellular-targeting in soybean (Glycine max) seeds with the goal of increasing palmitoleic acid (16:1Δ9), a high-valued fatty acid (FA), and simultaneously decreasing saturated FA in oil. The expression resulted in the conversion of palmitic acid (16:0) to 16:1Δ9 in soybean seeds. 16:1Δ9 and its elongation product cis-vaccenic acid (18:1Δ11) were increased to 17 % of the total fatty acids by plastid-targeted expression of the enzyme. Other lipid changes include the decrease of polyunsaturated FA and saturated FA, suggesting that a mechanism exists downstream in oil biosynthesis to compensate the FA alternation. This is the first time a cytosolic acyl-CoA-∆9 desaturase is functionally expressed in plastid and stronger activity was achieved than its cytosolic expression. The present study provides a new strategy for converting 16:0 to 16:1Δ9 by engineering acyl-CoA-Δ9 desaturase in commercialized oilseeds.
authors: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, 030801, China.
authors: Jin-Ai
authors: JA
authors: Xue
authors: Xue
authors: X
authors: Mao
authors: Zhi-Rong
authors: ZR
authors: Yang
authors: Yong-Mei
authors: YM
authors: Wu
authors: Xiao-Yun
authors: XY
authors: Jia
authors: Li
authors: L
authors: Zhang
authors: Ai-Qin
authors: AQ
authors: Yue
authors: Ji-Ping
authors: JP
authors: Wang
authors: Run-Zhi
authors: RZ
authors: Li
mesh_terms: D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D030821:Plants, Genetically Modified; D018087:Plastids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism*
chemical_list: D005229:Fatty Acids, Monounsaturated; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D044943:Fatty Acid Desaturases; C467900:acyl-CoA delta9-desaturase