title: Estimation of CpG coverage in whole methylome next-generation sequencing studies.
abstract: BACKGROUND
  Methylation studies are a promising complement to genetic studies of DNA sequence. However, detailed prior biological knowledge is typically lacking, so methylome-wide association studies (MWAS) will be critical to detect disease relevant sites. A cost-effective approach involves the next-generation sequencing (NGS) of single-end libraries created from samples that are enriched for methylated DNA fragments. A limitation of single-end libraries is that the fragment size distribution is not observed. This hampers several aspects of the data analysis such as the calculation of enrichment measures that are based on the number of fragments covering the CpGs.
  
  
  RESULTS
  We developed a non-parametric method that uses isolated CpGs to estimate sample-specific fragment size distributions from the empirical sequencing data. Through simulations we show that our method is highly accurate. While the traditional (extended) read count methods resulted in severely biased coverage estimates and introduces artificial inter-individual differences, through the use of the estimated fragment size distributions we could remove these biases almost entirely. Furthermore, we found correlations of 0.999 between coverage estimates obtained using fragment size distributions that were estimated with our method versus those that were "observed" in paired-end sequencing data.
  
  
  CONCLUSIONS
  We propose a non-parametric method for estimating fragment size distributions that is highly precise and can improve the analysis of cost-effective MWAS studies that sequence single-end libraries created from samples that are enriched for methylated DNA fragments.
authors: Center for Biomarker Research and Personalized Medicine, School of Pharmacy, Virginia Commonwealth University, 1112 East Clay Street, P.O. Box 980533, Richmond, VA 23298, USA. ejvandenoord@vcu.edu
authors: Edwin J C G
authors: EJ
authors: van den Oord
authors: Jozsef
authors: J
authors: Bukszar
authors: Gábor
authors: G
authors: Rudolf
authors: Srilaxmi
authors: S
authors: Nerella
authors: Joseph L
authors: JL
authors: McClay
authors: Lin Y
authors: LY
authors: Xie
authors: Karolina A
authors: KA
authors: Aberg
mesh_terms: D000818:Animals; D018899:CpG Islands; D019175:DNA Methylation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017422:Sequence Analysis, DNA / Q000379:methods*
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