title: Polyelectrolyte multilayer coating with two regulatory molecules on titanium: construction and its biological effects.
abstract: OBJECTIVE
  This study aimed at constructing a novel disulfide-crosslinked collagen I/hyaluronic acid polyelectrolyte multilayer (PEM) coating incorporated with bFGF and arginine-glycine-aspartic acid on titanium via the layer-by-layer technique, and evaluating its biological effects.
  
  
  METHODS
  The surface topography and components, thickness, degradation behaviors and bFGF release profiles of the PEM coating were investigated. The effects of the PEM coating on osteoprogenitor cell growth and bone implant interfacial binding strength in the rabbit femur model were evaluated separately.
  
  
  RESULTS
  The formation of disulfide bonds improved the stability of the PEM coating, resulting in a coating that can release bFGF in a slow and sustained manner. Biological evaluations revealed that the resultant PEM coating on titanium promoted various cell behaviors and enhanced the binding strength.
  
  
  CONCLUSIONS
  The employed cotreatment regimen enabled bFGF and arginine-glycine-aspartic acid to have a synergistic effect on the cell responses, which, in turn, improved the osseointegration.
authors: Department of Oral & Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, 395 Yan'an Road, Hangzhou 310006, China.
authors: Qiaojie
authors: Q
authors: Luo
authors: Ying
authors: Y
authors: Huang
authors: Xuliang
authors: X
authors: Deng
authors: Jianxiang
authors: J
authors: Zhang
authors: Xiaohui
authors: X
authors: Li
authors: Shifang
authors: S
authors: Zhao
authors: Xiaodong
authors: X
authors: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D005269:Femur / Q000293:injuries; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D009842:Oligopeptides / Q000008:administration & dosage* / Q000494:pharmacology; D016348:Osseointegration / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D004220:Disulfides; D009842:Oligopeptides; D016222:Fibroblast Growth Factor 2; D015675:Osteocalcin; C047981:arginyl-glycyl-aspartic acid; D006820:Hyaluronic Acid; D003094:Collagen; D014025:Titanium; D000469:Alkaline Phosphatase