title: Decreased expression of miR-126 correlates with metastatic recurrence of hepatocellular carcinoma.
abstract: The liver transplant (LT) situation represents an attractive model for studying hepatocellular carcinoma (HCC) metastasis. Based on microarray data, we previously found that miR-126 expression was lower in tumor tissues of patients with post-LT HCC recurrence compared with non-recurrence. In this study, we examined the expression of miR-126 in HCC samples from 68 patients who had undergone LT using quantitative real-time PCR and analyzed its correlation with clinicopathological features and prognosis of patients. Furthermore, we performed experimental analyses to explore the involvement of miR-126 in HCC metastasis. We found that miR-126 levels were lower in tumor tissues of patients with post-LT HCC recurrence in comparison to patients with no-recurrence (p = 0.009). Lower expression of miR-126 in HCC was associated significantly with tumor recurrence (p = 0.011) and poor survival (p = 0.009) of patients. Functional studies indicated that ectopic expression of miR-126 significantly inhibits HCC cells migration, invasion, proliferation and colony formation in vitro, and suppresses experimental lung colonization in vivo. Our study revealed that down-regulation of miR-126 plays an important role in HCC metastasis, and suggest a potential application of miR-126 in prognosis prediction and HCC treatment.
authors: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 85 Wujin Road, Shanghai, 200080, China.
authors: Hongyuan
authors: H
authors: Chen
authors: Ruizheng
authors: R
authors: Miao
authors: Junwei
authors: J
authors: Fan
authors: Zhongbo
authors: Z
authors: Han
authors: Junyi
authors: J
authors: Wu
authors: Guoqiang
authors: G
authors: Qiu
authors: Huamei
authors: H
authors: Tang
authors: Zhihai
authors: Z
authors: Peng
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D060888:Real-Time Polymerase Chain Reaction; D012008:Recurrence
chemical_list: C527111:MIRN126 microRNA, human; D035683:MicroRNAs
references: Nat Rev Clin Oncol. 2011 Feb;8(2):75-84
references: 21045789
references: Hepatology. 2001 Jun;33(6):1394-403
references: 11391528
references: Cell. 2009 Feb 20;136(4):586-91
references: 19239879
references: Cancer Lett. 2010 Dec 1;298(1):50-63
references: 20619534
references: Hepatology. 2010 Mar;51(3):846-56
references: 20054866
references: Nat Rev Cancer. 2006 Apr;6(4):259-69
references: 16557279
references: Lung Cancer. 2009 Nov;66(2):169-75
references: 19223090
references: Mol Cancer Res. 2012 Jan;10(1):3-10
references: 22064652
references: J Clin Oncol. 1999 Jan;17(1):324-31
references: 10458250
references: N Engl J Med. 1996 Mar 14;334(11):693-9
references: 8594428
references: Cell. 2009 Jun 12;137(6):1032-46
references: 19524507
references: Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5330-5
references: 19289845
references: Cell. 2004 Jan 23;116(2):281-97
references: 14744438
references: Cell. 2006 Nov 17;127(4):679-95
references: 17110329
references: Nature. 2007 Oct 11;449(7163):682-8
references: 17898713
references: J Clin Oncol. 2009 Dec 1;27(34):5848-56
references: 19884536
references: Nat Med. 2011 Aug 07;17(9):1101-8
references: 21822286
references: Br J Cancer. 2011 Jan 18;104(2):235-40
references: 21102580
references: Mol Oncol. 2012 Aug;6(4):445-57
references: 22552153
references: Hepatology. 2008 Mar;47(3):897-907
references: 18176954
references: Oncogene. 2008 Sep 18;27(42):5643-7
references: 18504431
references: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references: 15761078
references: Cell. 2009 Jan 23;136(2):215-33
references: 19167326
references: Nature. 2004 Sep 16;431(7006):350-5
references: 15372042
references: Gastroenterology. 2011 Jan;140(1):322-31
references: 20951699
references: Hepatol Res. 2009 Aug;39(8):786-94
references: 19473441
references: Cell. 2000 Jan 7;100(1):57-70
references: 10647931
references: Gastroenterology. 2007 Jun;132(7):2557-76
references: 17570226
references: Annu Rev Med. 2009;60:167-79
references: 19630570
references: Gastroenterology. 2008 Jul;135(1):257-69
references: 18555017
references: Nature. 2008 Jan 10;451(7175):147-52
references: 18185580