title: [Study on effect of coptidis rhizoma on red blood cells of normal mice and its antioxidant property].
abstract: OBJECTIVE
  To observe the effect of Coptidis Rhizoma (CR) on hemolysis and antioxidant system of normal mice and its impact on the functions, while evaluating the oxidation reduction property of CR and berberine.
  
  
  METHODS
  In the whole animal experiment, normal mice were orally administered with CR at the dose of 1.2 g x kg(-1) for three days. Their blood were collected to detect the hemoglobin in plasma, the content of serum bilirubin, the number of peripheral blood reticulocytes, the T-AOC in whole blood, measure the contents of glucose-6-phosphate-dehydrogenase (G6PD), superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) of RBC membrane, determine the activity of Na(+)-K(+)-ATPase, Ca(2+)-Mg(2+)-ATPase, fluidity, and observe its impact on the liquidity and deformability of RBCs. According to the electrical and biochemical experiment, the voltammetric behaviors of CR and berberine on glassy carbon electrode were evaluated using cyclic voltammetry. In the RBC in vitro experiment, the impact of Coptidis Rhizoma on autoxidation hemolysis rate of RBCs of normal mice was observed.
  
  
  RESULTS
  There was no significant effect on hemoglobin, serum bilirubin, and reticulocyte count in normal mice administrated with CR at the dose of 1.2 g x kg(-1), and so is on RBC membrane SOD, G6PD, MDA, GSH and whole blood T-AOC activity. In addition, CR had also no significant effect on Na(+)-K(+)-ATPase, Ca(2+)-Mg(2+)-ATPase activity, and no notable impact on the fluidity and deformability of RBCs. There were two oxidation peaks at -0.27 V and 0.60 V induced by CR and one oxidation peak induced by berberine at 0.56 V, with no reduction peak at fly-back. CR could significantly inhibit oxidative hemolysis in RBCs at the dose of 0.125-2 g x L(-1) in vitro.
  
  
  CONCLUSIONS
  The normal dose of Coptidis Rhizoma can not cause hemolysis of RBC, and also can not change antioxidant system and functions of RBC, CR and berberine show antioxidant (reducing) properties.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Ying
authors: Y
authors: Xu
authors: Chun-Fang
authors: CF
authors: Liu
authors: Bin
authors: B
authors: Yang
authors: Yan-Wei
authors: YW
authors: Wang
authors: Li
authors: L
authors: Qiao
authors: N
authors: N
authors: Lin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001599:Berberine / Q000494:pharmacology; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D008297:Male; D051379:Mice
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D001599:Berberine; C107393:Coptidis rhizoma extract