title: Cell cycle-dependent recruitment of polycomb proteins to the ASNS promoter counteracts C/ebp-mediated transcriptional activation in Bombyx mori.
abstract: Epigenetic modifiers and transcription factors contribute to developmentally programmed gene expression. Here, we establish a functional link between epigenetic regulation by Polycomb group (PcG) proteins and transcriptional regulation by C/ebp that orchestrates the correct expression of Bombyx mori asparagine synthetase (BmASNS), a gene involved in the biosynthesis of asparagine. We show that the cis-regulatory elements of YY1-binding motifs and the CpG island present on the BmASNS promoter are required for the recruitment of PcG proteins and the subsequent deposition of the epigenetic repression mark H3K27me3. RNAi-mediated knockdown of PcG genes leads to derepression of the BmASNS gene via the recruitment of activators, including BmC/ebp, to the promoter. Intriguingly, we find that PcG proteins and BmC/ebp can dynamically modulate the transcriptional output of the BmASNS target in a cell cycle-dependent manner. It will be essential to suppress BmASNS expression by PcG proteins at the G2/M phase of the cell cycle in the presence of BmC/ebp activator. Thus, our results provide a novel insight into the molecular mechanism underlying the recruitment and regulation of the PcG system at a discrete gene locus in Bombyx mori.
authors: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Fukuoka, Japan.
authors: Zhiqing
authors: Z
authors: Li
authors: Daojun
authors: D
authors: Cheng
authors: Hiroaki
authors: H
authors: Mon
authors: Li
authors: L
authors: Zhu
authors: Jian
authors: J
authors: Xu
authors: Tsuneyuki
authors: T
authors: Tatsuke
authors: Jae Man
authors: JM
authors: Lee
authors: Qingyou
authors: Q
authors: Xia
authors: Takahiro
authors: T
authors: Kusakabe
mesh_terms: D000818:Animals; D001216:Asparagine / Q000096:biosynthesis; D001217:Aspartate-Ammonia Ligase / Q000235:genetics* / Q000378:metabolism; D012831:Bombyx / Q000201:enzymology*; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000235:genetics; D018899:CpG Islands / Q000235:genetics; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D063146:Polycomb-Group Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D063146:Polycomb-Group Proteins; D001216:Asparagine; D001217:Aspartate-Ammonia Ligase
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