title: Complete identification of proteins responsible for human blood plasma fouling on poly(ethylene glycol)-based surfaces.
abstract: The resistance of poly(ethylene glycol) (PEG) against protein adsorption is crucial and has been widely utilized in various biomedical applications. In this work, the complete protein composition of biofilms deposited on PEG-based surfaces from human blood plasma (BP) was identified for the first time using nanoLC-MS/MS, a powerful tool in protein analysis. The mass of deposited BP and the number of different proteins contained in the deposits on individual surfaces decreased in the order of self-assembling monolayers of oligo(ethylene glycol) alkanethiolates (SAM) > poly(ethylene glycol) end-grafted onto a SAM > poly(oligo(ethylene glycol) methacrylate) brushes prepared by surface initiated polymerization (poly(OEGMA)). The BP deposit on the poly(OEGMA) surface was composed only of apolipoprotein A-I, apolipoprotein B-100, complement C3, complement C4-A, complement C4-B, histidine-rich glycoprotein, Ig mu chain C region, fibrinogen (Fbg), and serum albumin (HSA). The total resistance of the surface to the Fbg and HSA adsorption from single protein solutions suggested that their deposition from BP was mediated by some of the other proteins. Current theories of protein resistance are not sufficient to explain the observed plasma fouling. The research focused on the identified proteins, and the experimental approach used in this work can provide the basis for the understanding and rational design of plasma-resistant surfaces.
authors: Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, vvi, Prague, Czech Republic.
authors: Tomáš
authors: T
authors: Riedel
authors: Zuzana
authors: Z
authors: Riedelová-Reicheltová
authors: Pavel
authors: P
authors: Májek
authors: César
authors: C
authors: Rodriguez-Emmenegger
authors: Milan
authors: M
authors: Houska
authors: Jan E
authors: JE
authors: Dyr
authors: Eduard
authors: E
authors: Brynda
mesh_terms: D000327:Adsorption; D002853:Chromatography, Liquid; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D053719:Tandem Mass Spectrometry
chemical_list: D011506:Proteins; D011092:Polyethylene Glycols