title: Localization of oxidized low-density lipoprotein and its relation to plaque morphology in human coronary artery.
abstract: OBJECTIVE
  Oxidized low-density lipoprotein (oxLDL) plays a key role in the formation of atherosclerotic plaques. However, its localization in human coronary arterial wall is not well understood. The present study was performed to visualize deposition sites and patterns of native oxLDL and their relation to plaque morphology in human coronary artery.
  
  
  METHODS
  Evans blue dye (EB) elicits a violet fluorescence by excitation at 345-nm and emission at 420-nm, and a reddish-brown fluorescence by excitation at 470-nm and emission at 515-nm characteristic of oxLDL only. Therefore, native oxLDL in excised human coronary artery were investigated by color fluorescent microscopy (CFM) using EB as a biomarker.
  
  
  RESULTS
  (1) By luminal surface scan with CFM, the % incidence of oxLDL in 38 normal segments, 41 white plaques and 32 yellow plaques that were classified by conventional angioscopy, was respectively 26, 44 and 94, indicating significantly (p<0.05) higher incidence in the latter than the former two groups. Distribution pattern was classified as patchy, diffuse and web-like. Web-like pattern was observed only in yellow plaques with necrotic core. (2) By transected surface scan, oxLDL deposited within superficial layer in normal segments and diffusely within both superficial and deep layers in white and yellow plaques. In yellow plaques with necrotic core, oxLDL deposited not only in the marginal zone of the necrotic core but also in the fibrous cap.
  
  
  CONCLUSIONS
  Taken into consideration of the well-known process of coronary plaque growth, the results suggest that oxLDL begins to deposit in human coronary artery wall before plaque formation and increasingly deposits with plaque growth, exhibiting different deposition sites and patterns depending on morphological changes.
authors: Japan Foundation for Cardiovascular Research, Funabashi, Japan. uchiy@ta2.so-net.ne.jp
authors: Yasumi
authors: Y
authors: Uchida
authors: Yuko
authors: Y
authors: Maezawa
authors: Yasuto
authors: Y
authors: Uchida
authors: Nobuyuki
authors: N
authors: Hiruta
authors: Ei
authors: E
authors: Shimoyama
authors: Seiji
authors: S
authors: Kawai
mesh_terms: D003331:Coronary Vessels / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D058226:Plaque, Atherosclerotic / Q000473:pathology
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein
references: Prog Cardiovasc Dis. 2008 Nov-Dec;51(3):183-94
references: 19026853
references: J Clin Invest. 2011 Dec;121(12):4861-9
references: 22105168
references: Circulation. 2008 Jun 24;117(25):3206-15
references: 18541740
references: Arterioscler Thromb Vasc Biol. 1998 Feb;18(2):177-84
references: 9484981
references: Autoimmun Rev. 2008 Jul;7(7):558-66
references: 18625445
references: Cardiovasc Drugs Ther. 2009 Feb;23(1):31-40
references: 18949542
references: Circ J. 2010 Oct;74(10):2181-6
references: 20736502
references: Atherosclerosis. 1995 Apr 7;114(1):45-54
references: 7605375
references: PLoS One. 2010 Sep 02;5(9):
references: 20824093
references: Atherosclerosis. 1996 Sep 27;126(1):131-41
references: 8879441
references: Lab Invest. 2002 Oct;82(10):1437-47
references: 12379778
references: JACC Cardiovasc Imaging. 2010 Apr;3(4):398-408
references: 20394902
references: Circulation. 1995 Sep 15;92(6):1565-9
references: 7664441
references: Atherosclerosis. 2011 Aug;217(2):427-32
references: 21514591
references: Arterioscler Thromb Vasc Biol. 2011 Jun;31(6):1452-60
references: 21415387
references: J Nucl Cardiol. 1999 Jan-Feb;6(1 Pt 1):41-53
references: 10070840
references: J Vasc Surg. 2010 Sep;52(3):704-13
references: 20573470
references: Arterioscler Thromb Vasc Biol. 2009 Oct;29(10):1478-80
references: 19592469
references: Am Heart J. 2004 Nov;148(5):818-25
references: 15523312
references: Am Heart J. 1995 Nov;130(5):1114-9
references: 7484744
references: Circ J. 2011;75(8):1913-9
references: 21670541
references: Circulation. 2000 Nov 28;102(22):2680-6
references: 11094032
references: J Lipid Res. 2009 Sep;50(9):1735-43
references: 19359705
references: Arterioscler Thromb Vasc Biol. 2009 Jan;29(1):92-8
references: 18988891
references: PLoS One. 2012;7(11):e50678
references: 23209809
references: Med Electron Microsc. 2002 Dec;35(4):179-203
references: 12658354
references: J Lipid Res. 1998 Jul;39(7):1349-61
references: 9684737
references: J Clin Invest. 2009 Jan;119(1):136-45
references: 19065049
references: Biochim Biophys Acta. 2000 Sep 27;1487(2-3):233-45
references: 11018475
references: N Engl J Med. 1992 Jan 23;326(4):242-50
references: 1727977