title: Short-term response of mitomycin C on the human rectus muscle following strabismus surgery: histological, ultrastructural, and biomechanical evaluation.
abstract: This study investigated the inflammatory effect of intraoperative mitomycin C (MMC) on adhesion reformation in human rectus muscles. Ten consecutive patients who underwent medial rectus resection had their postoperative rectus muscles divided into two groups: control group (n = 10) and MMC group (n = 10). In the MMC group, the muscle was soaked for 2 min with MMC, prepared as a 0.2 mg/mL (0.02%) solution. The 0.02% MMC reactions were examined using histological analysis with hematoxylin-eosin (inflammatory response) and Masson's trichrome (collagen fibrils), immunoreactivities of cyclooxygenase-II (inflammatory response), and collagen type I and III, scanning electron microscopy analysis to quantify the diameter and D-periodicity of collagen fibrils, and atomic force microscopy analysis to quantify the diameter, D-periodicity, and adhesion force of collagen fibrils. The rectus muscles treated with 0.02% MMC showed a significantly increased inflammatory response (p < 0.05), increased collagen density (p < 0.0001), increased fibril diameter (p < 0.001 or p < 0.05), and decreased fibril adhesion force (p < 0.005) compared to the rectus muscles in the control group. MMC simultaneously caused an inflammatory response as well as nanostructural and biomechanical property changes in the collagen fibril network.
authors: Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea.
authors: Samjin
authors: S
authors: Choi
authors: Youjin
authors: Y
authors: Cheong
authors: Jae-Ho
authors: JH
authors: Shin
authors: Kyung A
authors: KA
authors: Kim
authors: Jae Beum
authors: JB
authors: Bang
authors: Kyung-Hyun
authors: KH
authors: Jin
authors: Hun-Kuk
authors: HK
authors: Park
mesh_terms: D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D016685:Mitomycin / Q000494:pharmacology*; D009801:Oculomotor Muscles / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D012155:Reticulin / Q000187:drug effects / Q000648:ultrastructure; D013285:Strabismus / Q000601:surgery
chemical_list: D012155:Reticulin; D016685:Mitomycin