title: Expression analysis of genes involved in TLR2-related signaling pathway: Inflammation and apoptosis after ischemic brain injury.
abstract: Toll-like receptor 2 (TLR2) is involved in innate immunity in the brain and in the cascade of events after ischemic stroke. The aim of this study was to get an insight into the expression of genes related to TLR2 signaling pathway and associated with inflammation and apoptosis in the later stages of brain response after ischemic injury. Middle cerebral artery occlusion was performed on both wild-type and TLR2(-/-) mice followed by real-time PCR to measure the relative expression of selected genes. In TLR2(-/-) mice expression of genes involved in proinflammatory response was decreased after cerebral ischemia. Tnf was the most prominent cytokine active in the late phase of recovery. Contrary to proinflammatory genes, the expression of Casp8, as a hallmark of apoptosis, was increased in TLR2(-/-) mice, in particular in the late phase of recovery.
authors: Laboratory for Neurogenetics and Developmental Genetics, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Salata 12, Zagreb HR-10000, Croatia. illumielle@gmail.com
authors: L
authors: L
authors: Winters
authors: T
authors: T
authors: Winters
authors: D
authors: D
authors: Gorup
authors: D
authors: D
authors: Mitrečić
authors: M
authors: M
authors: Curlin
authors: J
authors: J
authors: Križ
authors: S
authors: S
authors: Gajović
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007249:Inflammation / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D015398:Signal Transduction / Q000235:genetics*; D020521:Stroke / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D051195:Toll-Like Receptor 2; D053181:Caspase 8