title: Electron transfer between multihaem cytochromes c₃ from Desulfovibrio africanus.
abstract: The tetrahaem type I cytochromes c3 from Desulfovibrionaceae shuttle electrons from a periplasmic hydrogenase to transmembrane electron transfer complexes. In D. africanus, it is believed that the electrons are received by another tetrahaem cytochrome c3, denoted type II, which is associated with the membrane complex. Thermodynamic measurements show that the type I cytochrome c3 has the potential to transfer two electrons at a time. This study uses two-dimensional NMR to investigate the exchange of electrons between type I and type II cytochromes c3 at equilibrium in intermediate stages of oxidation. The results indicate that the two proteins are physiological partners but that only single-electron transfers occur in solution.
authors: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da, República, 2780-157 Oeiras, Portugal.
authors: Pedro O
authors: PO
authors: Quintas
authors: Márcia S
authors: MS
authors: Oliveira
authors: Teresa
authors: T
authors: Catarino
authors: David L
authors: DL
authors: Turner
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism; D045284:Desulfovibrio africanus / Q000378:metabolism*; D004579:Electron Transport; D004583:Electrons; D006418:Heme / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010084:Oxidation-Reduction; D019897:Periplasm; D013816:Thermodynamics
chemical_list: D003574:Cytochrome c Group; D006418:Heme; C017165:cytochrome c(3)