title: Anti-ovarian cancer potential of two acidic polysaccharides from the rhizoma of Menispermum dauricum.
abstract: Two acidic polysaccharides (MDP-A1 and MDP-A2) were isolated from the rhizome of Menispermum dauricum and their apparent molecular weight are 9.1×10(4) and 5.8×10(4) Da, respectively. Both contained glucose, mannose, galactose, arabinose, glucuronic acid and galacturonic acid, but differed in the molar ratio. We also investigated the antitumor activities and mechanisms of MDP-A1 and MDP-A2 in human ovarian carcinoma SKOV3 cells. The MTT assay showed that MDP-A1 and MDP-A2 were able to inhibit cell proliferation of SKOV3 cells in a bell-shaped concentration-response manner, due to a significant increase in the number of apoptotic cells. Furthermore, treatment with MDP-A1 or MDP-A2 caused significant induction of caspase-3 and caspase-8, but slightly affected caspase-9 activity. In addition, a significant reduction in tumor volume was observed in mice treated with MDP-A1 or MDP-A2. Taken together, these results suggested that MDP-A1 and MDP-A2 had a potential application as natural antitumor drugs.
authors: Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors: Mei
authors: M
authors: Lin
authors: Bairong
authors: B
authors: Xia
authors: Meng
authors: M
authors: Yang
authors: Shu
authors: S
authors: Gao
authors: Yanqiu
authors: Y
authors: Huo
authors: Ge
authors: G
authors: Lou
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D031603:Menispermum / Q000737:chemistry*; D051379:Mice; D010051:Ovarian Neoplasms / Q000473:pathology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D020169:Caspases