title: [Arsenic trioxide enhances TRAIL inducing human lung cancer cell line A549 cells apoptosis by down-regulate the expression of NF-kappaB].
abstract: OBJECTIVE
  To study the influence of arsenic trioxide combined with human tumor necrosis factor related apoptosis inducing ligand (TRAIL) on the apoptosis and the expression of NF-kappaB of human non-small-cell lung cancer cell line.
  
  
  METHODS
  The proliferation of human non-small-cell lung cancer cell line A549 cultured in vitro were treated by As2O3, TRAIL alone and combined. The cell proliferation was detected by the assay of MTT, flow cytometry with PI stain was used to detect the apoptosis rate, NF-kappaB mRNA level of A549 cells were detected by RT-PCR. The expression of NF-kappaB protein were detected by Western blot. The activity of NF-kappaB was measured by ELISA.
  
  
  RESULTS
  Compared with As2O3 alone, As2O3 combined with TRAIL could increase the inhibition and apoptosis ratio significantly (P<0.05). The expression of NF-kappaB in combined group was obviously less than that in As2O3 alone and control; the activity of NF-kappaB was inhibited by combined groups. The NF-kappaB mRNA and protein expression and the activity of NF-kappaB were separately negative related with the apoptosis ratio (P<0.05).
  
  
  CONCLUSIONS
  As2O3 can enhance TRAIL inducing of human lung cancer cell lines A549 apoptosis by inhibition of NF-kappaB signaling pathway.
authors: Department of Respiratory, the Affiliated Hospital of Guilin Medical College, Guilin 541001, China.
authors: Ji-Ying
authors: JY
authors: Wang
authors: Xue-Qiang
authors: XQ
authors: Zhao
authors: Chang-Ming
authors: CM
authors: Wang
authors: Bi-Wen
authors: BW
authors: Mo
authors: Ming
authors: M
authors: Jiang
authors: Feng
authors: F
authors: Chen
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D016328:NF-kappa B; D010087:Oxides; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D000077237:Arsenic Trioxide