title: MAGE-A3 is highly expressed in a subset of colorectal cancer patients.
abstract: The expression of Cancer/Testis (CT) antigens in some tumors and restricted expression in normal tissue make CT antigens attractive vaccine targets. We evaluated the expression of MAGE-A3, PLAC1, GAGE, and CTAG2 in a series of colorectal cancers (CRC). CT mRNA expression was determined via quantitative PCR on paired tumors and normal tissue samples from 82 CRC patients. In addition, plasma antibody titers specific to MAGE-A3, PLAC1, GAGE, and CTAG2 were determined via ELISA. Tissue expression of MAGE-A3 was assessed via a standard IHC protocol. The Student's t-test was used for statistical analysis (significance p < 0.05). Tumor expression of MAGE-A3, CTAG2, and GAGE was compared to the levels of expression in testis. The percentage of samples that had a tumor vs. testis expression ratio above 0.1% was: MAGE-A3 (28%) and CTAG2 (17%) but no tumor presented GAGE expression levels above 0.1%. The expression levels of PLAC1 in tumors were compared to the levels in placenta, and in 12.8% of the samples analyzed, these levels were above 0.1%. Sero-reactivity specific for MAGE-A genes and PLAC1 was noted in 2.4% and 2.6% of patients, respectively. MAGE-A3 and PLAC1 may hold promise as vaccine targets for CRC. Further study is warranted.
authors: Division of Colon and Rectal Surgery, Department of Surgery, St Luke's-Roosevelt Hospital Center, New York, NY 10019, USA.
authors: H M C
authors: HM
authors: Shantha Kumara
authors: Michael J
authors: MJ
authors: Grieco
authors: Otavia L
authors: OL
authors: Caballero
authors: Tao
authors: T
authors: Su
authors: Aqeel
authors: A
authors: Ahmed
authors: Erika
authors: E
authors: Ritter
authors: Sacha
authors: S
authors: Gnjatic
authors: Vesna
authors: V
authors: Cekic
authors: Lloyd J
authors: LJ
authors: Old
authors: Andrew J
authors: AJ
authors: Simpson
authors: Carlos
authors: C
authors: Cordon-Cardo
authors: Richard L
authors: RL
authors: Whelan
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009367:Neoplasm Staging; D011257:Pregnancy Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; C510162:CTAG2 protein, human; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins; C415583:PLAC1 protein, human; D011257:Pregnancy Proteins
keywords: Cancer/Testis antigens; MAGE-A3; colorectal cancer; tumor expression
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