title: Allopolyploidy has a moderate impact on restructuring at three contrasting transposable element insertion sites in resynthesized Brassica napus allotetraploids.
abstract: The role played by whole-genome duplication (WGD) in evolution and adaptation is particularly well illustrated in allopolyploids, where WGD is concomitant with interspecific hybridization. This 'Genome Shock', usually accompanied by structural and functional modifications, has been associated with the activation of transposable elements (TEs). However, the impact of allopolyploidy on TEs has been studied in only a few polyploid species, and not in Brassica, which has been marked by recurrent polyploidy events. Here, we developed sequence-specific amplification polymorphism (SSAP) markers for three contrasting TEs, and compared profiles between resynthesized Brassica napus allotetraploids and their diploid Brassica progenitors. To evaluate restructuring at TE insertion sites, we scored changes in SSAP profiles and analysed a large set of differentially amplified SSAP bands. No massive structural changes associated with the three TEs surveyed were detected. However, several transposition events, specific to the youngest TE originating from the B. oleracea genome, were identified. Our study supports the hypothesis that TE responses to allopolyploidy are highly specific. The changes observed in SSAP profiles lead us to hypothesize that they may partly result from changes in DNA methylation, questioning the role of epigenetics during the formation of a new allopolyploid genome.
authors: AgroParisTech, 16 rue Claude Bernard, F-75231 Paris Cedex 05, France.|CNRS, UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Véronique
authors: V
authors: Sarilar
authors: Université Paris-Sud, UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Paulina Martinez
authors: PM
authors: Palacios
authors: INRA, UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Agnès
authors: A
authors: Rousselet
authors: INRA, UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Céline
authors: C
authors: Ridel
authors: INRA, UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Matthieu
authors: M
authors: Falque
authors: INRA, UMR 1349 IGEPP, BP 35327, F-35653 Le Rheu Cedex, France.
authors: Frédérique
authors: F
authors: Eber
authors: INRA, UMR 1349 IGEPP, BP 35327, F-35653 Le Rheu Cedex, France.
authors: Anne-Marie
authors: AM
authors: Chèvre
authors: INRA, UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Johann
authors: J
authors: Joets
authors: AgroParisTech, 16 rue Claude Bernard, F-75231 Paris Cedex 05, France.|UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Philippe
authors: P
authors: Brabant
authors: AgroParisTech, 16 rue Claude Bernard, F-75231 Paris Cedex 05, France.|UMR 0320/8120 Génétique Végétale, Ferme du Moulon, F-91190 Gif-sur-Yvette, France.
authors: Karine
authors: K
authors: Alix
mesh_terms: D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics*; D003433:Crosses, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D004171:Diploidy; D016254:Mutagenesis, Insertional / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011123:Polyploidy; D015203:Reproducibility of Results
chemical_list: D004251:DNA Transposable Elements
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