title: The role of the ethylacetate fraction from hydnocarpi semen in acute inflammation in vitro model.
abstract: We previously reported that Hydnocarpi Semen (HS) has a wound healing effect on diabetic foot ulcer lesion in mice. In this study, ethylacetate (EtOAc) fraction from HS extract were evaluated for their wound healing activity by using in vitro acute inflammation model. GC and GC/MS analysis shows that the main constituents in EtOAc fraction are chaulmoogric acid, hydnocarpic acid, and gorlic acid. EtOAc fraction activated macrophages to increase the production of TNF-α. The fraction also increased the production of TGF-β and VEGF, which induced fibroblast activation and angiogenesis. These results suggest that the mechanism that the fraction helps to enhance healing of skin wound is possibly associated with the production of TNF-α, as well as secretion of VEGF, TGF-β and HS may have a new bioactive material for the treatment of skin wound.
authors: College of Pharmacy, Sahmyook University, Seoul 139-742, Korea. ; Institute of Chronic Disease, Sahmyook University, Seoul 139-742, Korea.
authors: Geum Seon
authors: GS
authors: Lee
authors: Hong
authors: H
authors: Shim
authors: Ki-Man
authors: KM
authors: Lee
authors: Seung Hyun
authors: SH
authors: Kim
authors: Dongsool
authors: D
authors: Yim
authors: Jae Hoon
authors: JH
authors: Cheong
authors: Tae Jin
authors: TJ
authors: Kang
keywords: Cytokines; Ethylacetate fraction; Hydnocarpi Semen; Inflammation
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