title: Concise synthetic routes to human insulin.
abstract: We report a set of concise and efficient routes for the chemical synthesis of human insulin using a two- or three-step combination procedure that employs Trt, Acm, and t-Bu cysteine protection schemes. Starting with resin-bound assembled A and B chains, human insulin can be obtained within the span of a single work day in 5.4% overall yield based on the crude A or B chain.
authors: Lilly Research Laboratories, Indianapolis, Indiana 46285, United States. liufx@lilly.com
authors: Fa
authors: F
authors: Liu
authors: Ethan Y
authors: EY
authors: Luo
authors: David B
authors: DB
authors: Flora
authors: John P
authors: JP
authors: Mayer
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D004220:Disulfides; D006801:Humans; D007328:Insulin / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004220:Disulfides; D007328:Insulin; D003545:Cysteine