title: Direct differentiation of hepatic stem-like WB cells into insulin-producing cells using small molecules.
abstract: Recent evidence suggests that experimental induction of hepatocytes into pancreatic cells provides new cell transplantation therapy prospects for type 1 diabetes mellitus. Stepwise differentiation from rat liver epithelial stem-like WB-F344 cells (WB cells) into functional insulin-secreting cells will identify key steps in β-cell development and may yet prove useful for transplantation therapy for diabetic patients. An essential step in this protocol was the generation of pancreatic precursor cell that express Pdx1 based on induction by a combination of 5-aza-2'-deoxycytidine, trichostatin A, retinoic acid, and a mix of insulin, transferrin and selenite. The Pdx1-expressing cells express other pancreatic markers and contribute to endocrine cells in vitro and in vivo. This study indicates an efficient chemical protocol for differentiating WB cells into functional insulin-producing cells using small molecules, and represents a promising hepatocyte-based treatment for diabetes mellitus.
authors: Department of Endocrinology, Chinese PLA General Hospital, Beijing, China.
authors: Jianping
authors: J
authors: Liu
authors: Yanmei
authors: Y
authors: Liu
authors: Honggang
authors: H
authors: Wang
authors: Haojie
authors: H
authors: Hao
authors: Qingwang
authors: Q
authors: Han
authors: Jing
authors: J
authors: Shen
authors: Jun
authors: J
authors: Shi
authors: Chunlin
authors: C
authors: Li
authors: Yiming
authors: Y
authors: Mu
authors: Weidong
authors: W
authors: Han
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D015870:Gene Expression; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000637:transplantation; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D051381:Rats; D018038:Sodium Selenite / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D015534:Trans-Activators / Q000378:metabolism; D014168:Transferrin / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D018398:Homeodomain Proteins; D006877:Hydroxamic Acids; D007328:Insulin; D015534:Trans-Activators; D014168:Transferrin; C084010:pancreatic and duodenal homeobox 1 protein; C012589:trichostatin A; D014212:Tretinoin; D018038:Sodium Selenite
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