title: Effects of surface-bound and intravenously administered heparin on cell-surface interactions: inflammation and coagulation.
abstract: Intravenous administration of heparin and heparin-bonded extracorporeal circuits are frequently used to mitigate the deleterious effects of blood contact with synthetic materials. The work described here utilized human blood in a micro-perfusion circuit to experimentally examine the effects of intravenous and surface-bound heparin on cellular activation. Activation markers of coagulation and of the inflammatory response were examined using flow cytometry; specifically, markers of platelet, monocyte, polymorphonuclear leukocyte (PMN), and lymphocyte activation were quantified. The results indicate that surface-bound heparin reduces the inflammatory response whereas systemically administered heparin does not. This finding has important implications for blood-contacting devices, particularly within the context of recently elucidated connections between inflammation pathways and coagulation disorders. Data presented indicate that surface-bound heparin and intravenously administered heparin play distinct, but vital roles in rendering biomaterial surfaces compatible with blood.
authors: Ension, Inc, Pittsburgh, PA 15238, USA. gjohnson@ension.com
authors: G
authors: G
authors: Johnson
authors: B
authors: B
authors: Curry
authors: L
authors: L
authors: Cahalan
authors: R
authors: R
authors: Prater
authors: J
authors: J
authors: Biggerstaff
authors: A
authors: A
authors: Hussain
authors: M
authors: M
authors: Gartner
authors: P
authors: P
authors: Cahalan
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D020099:Coated Materials, Biocompatible; D005112:Extracorporeal Circulation; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation; D008213:Lymphocyte Activation / Q000187:drug effects*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects*; D013499:Surface Properties
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D006493:Heparin