title: Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil.
abstract: A methanogenic archaeon, strain SMA-21(T), was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3-2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21(T) were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %). On basis of the level of DNA-DNA hybridization (22.1 %) between strain SMA-21(T) and Methanosarcina mazei DSM 2053(T) as well as of phenotypic and genotypic differences, strain SMA-21(T) was assigned to a novel species of the genus Methanosarcina, for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21(T) (=DSM 26065(T) [corrected] = JCM 18468).
authors: Helmholtz Centre Potsdam, German Research Centre for Geosciences (GFZ), Section 4.5 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.|Alfred Wegener Institute for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany.
authors: Dirk
authors: D
authors: Wagner
authors: Alfred Wegener Institute for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany.
authors: Janosch
authors: J
authors: Schirmack
authors: University of Tromsø, Department for Arctic and Marine Biology, Dramsveien 201, 9037 Tromsø, Norway.
authors: Lars
authors: L
authors: Ganzert
authors: Alfred Wegener Institute for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany.
authors: Daria
authors: D
authors: Morozova
authors: Helmholtz Centre Potsdam, German Research Centre for Geosciences (GFZ), Section 4.3 Organic Geochemistry, Telegrafenberg, 14473 Potsdam, Germany.
authors: Kai
authors: K
authors: Mangelsdorf
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D017020:Methanosarcina / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012800:Siberia; D012988:Soil Microbiology
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S