title: UDP-sugar pyrophosphorylase is essential for arabinose and xylose recycling, and is required during vegetative and reproductive growth in Arabidopsis.
abstract: Numerous nucleotide sugars are needed in plants to synthesize cell wall polymers and glycoproteins. The de novo synthesis of nucleotide sugars is of major importance. During growth, however, some polymers are broken down to monosaccharides. Reactivation of these sugars into nucleotide sugars occurs in two steps: first, by a substrate-specific sugar-1-kinase and, second, by UDP-sugar-pyrophosphorylase (USP), which has broad substrate specificity. A knock-out of the USP gene results in non-fertile pollen. By using various genetic complementation approaches we obtained a strong (>95%) knock-down line in USP that allowed us to investigate the physiological role of the enzyme during the life cycle. Mutant plants show an arabinose reduction in the cell wall, and accumulate mainly two sugars, arabinose and xylose, in the cytoplasm. The arabinogalactanproteins in usp mutants show no significant reduction in size. USP is also part of the myo-inositol oxygenation pathway to UDP-glucuronic acid; however, free glucuronic acid does not accumulate in cells, suggesting alternative conversion pathways of this monosaccharide. The knock-down plants are mostly sterile because of the improper formation of anthers and pollen sacks.
authors: Department Cell Biology, Plant Physiology, University of Salzburg, Hellbrunnerstr. 34, Salzburg, 5020, Austria.
authors: Claudia
authors: C
authors: Geserick
authors: Raimund
authors: R
authors: Tenhaken
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001089:Arabinose / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D014994:Xylose; D001089:Arabinose; D009713:Nucleotidyltransferases; C514559:UDP-sugar pyrophosphorylase, Arabidopsis
references: Plant Cell Physiol. 2012 May;53(5):921-9
references: 22437845
references: Plant J. 2008 Nov;56(3):351-63
references: 18644001
references: Plant Physiol. 2003 Oct;133(2):462-9
references: 14555774
references: Plant J. 2007 Oct;52(2):252-62
references: 17764499
references: Science. 1968 Jun 21;160(3834):1352-4
references: 5651896
references: FEBS J. 2007 Jan;274(1):1-22
references: 17222174
references: Plant Physiol. 1999 Oct;121(2):383-9
references: 10517829
references: Plant Cell. 2007 Mar;19(3):847-61
references: 17400897
references: Plant Physiol. 1957 May;32(3):212-5
references: 16654979
references: Plant Physiol. 1991 Aug;96(4):1255-60
references: 16668327
references: J Biol Chem. 2004 Oct 29;279(44):45728-36
references: 15326166
references: Plant Physiol. 1978 Jan;61(1):96-100
references: 16660246
references: Curr Opin Plant Biol. 2004 Jun;7(3):277-84
references: 15134748
references: Plant Cell. 2008 Aug;20(8):2160-76
references: 18757557
references: Curr Opin Plant Biol. 2008 Jun;11(3):236-43
references: 18486535
references: Plant Physiol. 1999 Oct;121(2):535-43
references: 10517845
references: J Biol Chem. 2010 Jan 29;285(5):2902-10
references: 19951951
references: Plant J. 2005 Aug;43(4):568-85
references: 16098110
references: Science. 2003 Aug 1;301(5633):653-7
references: 12893945
references: Planta. 2011 Jul;234(1):157-69
references: 21394467
references: Biochem J. 2010 Aug 1;429(3):533-43
references: 20482518
references: Plant Physiol. 1978 Jan;61(1):89-95
references: 16660245
references: Plant Physiol. 2004 Mar;134(3):912-8
references: 15020755
references: Planta. 2006 Aug;224(3):520-32
references: 16557401
references: Plant Physiol. 1996 Nov;112(3):1127-34
references: 8938413
references: Plant Physiol Biochem. 2006 Apr;44(4):171-80
references: 16757173
references: Plant Physiol. 1972 Jul;50(1):7-14
references: 16658136
references: Biosci Biotechnol Biochem. 2007 Mar;71(3):761-71
references: 17341835
references: Plant Physiol. 2004 Dec;136(4):4256-64
references: 15563616
references: J Biol Chem. 2011 Nov 18;286(46):39982-92
references: 21949134
references: Planta. 2005 May;221(2):243-54
references: 15660207
references: Annu Rev Plant Biol. 2011;62:127-55
references: 21370975
references: Plant Cell. 2003 Feb;15(2):523-31
references: 12566589