title: Capillary electrophoresis for the analysis of glycosaminoglycan-derived disaccharides.
abstract: Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.
authors: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
authors: Yuqing
authors: Y
authors: Chang
authors: Bo
authors: B
authors: Yang
authors: Amanda
authors: A
authors: Weyers
authors: Robert J
authors: RJ
authors: Linhardt
mesh_terms: D000585:Aminacrine / Q000737:chemistry; D002138:Calibration; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods / Q000592:standards; D006025:Glycosaminoglycans / Q000737:chemistry*; D012015:Reference Standards
chemical_list: D004187:Disaccharides; D006025:Glycosaminoglycans; D000585:Aminacrine
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