title: Single-cell photoacoustic thermometry.
abstract: A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.
authors: Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, MO 63130, USA.
authors: Liang
authors: L
authors: Gao
authors: Lidai
authors: L
authors: Wang
authors: Chiye
authors: C
authors: Li
authors: Yan
authors: Y
authors: Liu
authors: Haixin
authors: H
authors: Ke
authors: Chi
authors: C
authors: Zhang
authors: Lihong V
authors: LV
authors: Wang
mesh_terms: D001831:Body Temperature; D006367:HeLa Cells; D006801:Humans; D006979:Hyperthermia, Induced; D033401:Microscopy, Acoustic / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000503:physiopathology / Q000628:therapy; D055096:Optical Devices; D055620:Optical Phenomena; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D062125:Thermometry / Q000295:instrumentation / Q000379:methods*
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