title: Racemization of the aspartic acid residue of amyloid-β peptide by a radical reaction.
abstract: Human amyloid-β peptide 1-42 (Aβ) was subjected to a radical reaction by using ascorbic acid and CuCl(2). The percentage of D-aspartic acid (D-Asp) after 24 h had increased to 6.69 ± 0.09%, this being comparable with the reported D-Asp concentration of purified core amyloids in Alzheimer's disease patients. This racemization was significantly inhibited by radical scavengers. L-Alanine was also racemized during the same reaction.
authors: Department of Food Science and Nutrition, Nara Women's University, Nara, Japan.
authors: Koharu
authors: K
authors: Tambo
authors: Tomomi
authors: T
authors: Yamaguchi
authors: Keiko
authors: K
authors: Kobayashi
authors: Eri
authors: E
authors: Terauchi
authors: Ikuyo
authors: I
authors: Ichi
authors: Shosuke
authors: S
authors: Kojo
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry*; D001224:Aspartic Acid / Q000737:chemistry*; D002839:Chromans / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000037:antagonists & inhibitors; D006801:Humans; D008623:Mercaptoethanol / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry*; D012996:Solutions; D013237:Stereoisomerism
chemical_list: D016229:Amyloid beta-Peptides; D002839:Chromans; D016166:Free Radical Scavengers; D005609:Free Radicals; D010446:Peptide Fragments; D012996:Solutions; C075222:amyloid beta-protein (1-42); D001224:Aspartic Acid; D008623:Mercaptoethanol; D003300:Copper; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; C029892:cupric chloride