title: The Janthinobacterium sp. HH01 genome encodes a homologue of the V. cholerae CqsA and L. pneumophila LqsA autoinducer synthases.
abstract: Janthinobacteria commonly form biofilms on eukaryotic hosts and are known to synthesize antibacterial and antifungal compounds. Janthinobacterium sp. HH01 was recently isolated from an aquatic environment and its genome sequence was established. The genome consists of a single chromosome and reveals a size of 7.10 Mb, being the largest janthinobacterial genome so far known. Approximately 80% of the 5,980 coding sequences (CDSs) present in the HH01 genome could be assigned putative functions. The genome encodes a wealth of secretory functions and several large clusters for polyketide biosynthesis. HH01 also encodes a remarkable number of proteins involved in resistance to drugs or heavy metals. Interestingly, the genome of HH01 apparently lacks the N-acylhomoserine lactone (AHL)-dependent signaling system and the AI-2-dependent quorum sensing regulatory circuit. Instead it encodes a homologue of the Legionella- and Vibrio-like autoinducer (lqsA/cqsA) synthase gene which we designated jqsA. The jqsA gene is linked to a cognate sensor kinase (jqsS) which is flanked by the response regulator jqsR. Here we show that a jqsA deletion has strong impact on the violacein biosynthesis in Janthinobacterium sp. HH01 and that a jqsA deletion mutant can be functionally complemented with the V. cholerae cqsA and the L. pneumophila lqsA genes.
authors: Abteilung für Mikrobiologie und Biotechnologie, Biozentrum Klein Flottbek, Universität Hamburg, Hamburg, Germany.
authors: Claudia
authors: C
authors: Hornung
authors: Anja
authors: A
authors: Poehlein
authors: Frederike S
authors: FS
authors: Haack
authors: Martina
authors: M
authors: Schmidt
authors: Katja
authors: K
authors: Dierking
authors: Andrea
authors: A
authors: Pohlen
authors: Hinrich
authors: H
authors: Schulenburg
authors: Melanie
authors: M
authors: Blokesch
authors: Laure
authors: L
authors: Plener
authors: Kirsten
authors: K
authors: Jung
authors: Andreas
authors: A
authors: Bonge
authors: Ines
authors: I
authors: Krohn-Molt
authors: Christian
authors: C
authors: Utpatel
authors: Gabriele
authors: G
authors: Timmermann
authors: Eva
authors: E
authors: Spieck
authors: Andreas
authors: A
authors: Pommerening-Röser
authors: Edna
authors: E
authors: Bode
authors: Helge B
authors: HB
authors: Bode
authors: Rolf
authors: R
authors: Daniel
authors: Christel
authors: C
authors: Schmeisser
authors: Wolfgang R
authors: WR
authors: Streit
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives / Q000235:genetics; D007783:Lactones; D016952:Legionella pneumophila / Q000201:enzymology / Q000235:genetics*; D042802:Oxalobacteraceae / Q000235:genetics*; D010802:Phylogeny; D053038:Quorum Sensing / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014734:Vibrio cholerae / Q000201:enzymology / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D007783:Lactones; C085427:LuxI protein, Bacteria; C091088:N-octanoylhomoserine lactone; D014157:Transcription Factors; D006714:Homoserine
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