title: An extract from wax apple (Syzygium samarangense (Blume) Merrill and Perry) effects glycogenesis and glycolysis pathways in tumor necrosis factor-α-treated FL83B mouse hepatocytes.
abstract: FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent D-glucose derivative, was performed, and the metabolism of carbohydrates was evaluated by examining the expression of glycogenesis or glycolysis-related proteins in insulin-resistant hepatocytes. The results show that WAE significantly improves the uptake of glucose and enhances glycogen content in insulin-resistant FL83B mouse hepatocytes. The results from Western blot analysis also reveal that WAE increases the expression of glycogen synthase (GS), hexokinase (HXK), glucose-6-phosphate dehydrogenase (G6PD), phosphofructokinase (PFK) and aldolase in TNF-α treated cells, indicating that WAE may ameliorate glucose metabolism by promoting glycogen synthesis and the glycolysis pathways in insulin-resistant FL83B mouse hepatocytes.
authors: Department of Human Development and Family Studies, National Taiwan Normal University, Taipei 10610, Taiwan. scs@ntnu.edu.tw
authors: Szu-Chuan
authors: SC
authors: Shen
authors: Wen-Chang
authors: WC
authors: Chang
authors: Chiao-Li
authors: CL
authors: Chang
mesh_terms: D000818:Animals; D002460:Cell Line; D005638:Fruit / Q000737:chemistry; D006003:Glycogen / Q000096:biosynthesis*; D006006:Glycogen Synthase / Q000032:analysis / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D007333:Insulin Resistance; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D027842:Syzygium / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D006003:Glycogen; D006006:Glycogen Synthase
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