title: The mechanism of effective electroacupuncture on T cell response in rats with experimental autoimmune encephalomyelitis.
abstract: Previously, we demonstrated that electroacupuncture (EA) decreased lymphocyte infiltration into the spinal cords of rats presenting with experimental autoimmune encephalomyelitis (EAE), a disease model used in the study of multiple sclerosis (MS). The aim of this study was to characterize the effects of EA on the EAE. Female Lewis rats were divided into either CFA, EAE, EA, or injection with naloxone after electroacupuncture (NAL) groups. Electroacupuncture was administered every day for 21 days. To evaluate proliferation and apoptosis, lymphocytes from rats presenting with EAE were collected and cultured with β-endorphin. Immunohistochemisty, flow cytometry and radio-immunity methods were applied to detect the expression of β-endorphin. Results presented in this report demonstrate that the beneficial anti-inflammatory effects of EA on EAE were related to β-endorphin production that balances the Thl/Th2 and Th17/Treg responses. These results suggest that β-endorphin could be an important component in the development of EA-based therapies used for the treatment of EAE.
authors: Department of Neurobiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin, China.
authors: Yumei
authors: Y
authors: Liu
authors: Hongwei
authors: H
authors: Wang
authors: Xinyue
authors: X
authors: Wang
authors: Lili
authors: L
authors: Mu
authors: Qingfei
authors: Q
authors: Kong
authors: Dandan
authors: D
authors: Wang
authors: Jinghua
authors: J
authors: Wang
authors: Yao
authors: Y
authors: Zhang
authors: Jinfeng
authors: J
authors: Yang
authors: Mingyan
authors: M
authors: Zhou
authors: Guangyou
authors: G
authors: Wang
authors: Bo
authors: B
authors: Sun
authors: Hulun
authors: H
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D015671:Electroacupuncture; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D005260:Female; D006801:Humans; D009103:Multiple Sclerosis / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D009270:Naloxone / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology; D058408:Th1-Th2 Balance / Q000187:drug effects; D058504:Th17 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D001615:beta-Endorphin / Q000494:pharmacology
chemical_list: D009270:Naloxone; D001615:beta-Endorphin
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