title: Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay.
abstract: Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA. Cross-reactivity levels of the mAb to HT-2, 15-acetyl-DON, and nivalenol were 0.1, 0.42, and 0.40%. The intra- and interassay precision coefficient variation (CV) were both <10%. In the mAb-coated ELISA, mean DON recovery rates in animal feed (0 to 1,000 mg/kg) ranged from 68.34 to 95.49% (CV; 4.10 to 13.38%). DON in a buffer solution (250, 500 and 1,000 ng/mL) was isolated using 300 mg of NVRQS-DON and 3 mg of magnetic nanoparticles (MNPs). The mean recovery rates of DON using this mAb-MNP system were 75.2, 96.9, and 88.1% in a buffer solution spiked with DON (250, 500, and 1,000 ng/mL). Conclusively we developed competitive ELISAs for detecting DON in animal feed and created a new tool for DON extraction using mAb-coupled MNPs.
authors: Animal and Plant Quarantine Agency, Anyang 430-757, Korea.
authors: Hyuk-Mi
authors: HM
authors: Lee
authors: Sung-Ok
authors: SO
authors: Song
authors: Sang-Ho
authors: SH
authors: Cha
authors: Sung-Bok
authors: SB
authors: Wee
authors: Karyn
authors: K
authors: Bischoff
authors: Sung-Won
authors: SW
authors: Park
authors: Seong-Wan
authors: SW
authors: Son
authors: Hwan-Goo
authors: HG
authors: Kang
authors: Myung-Haing
authors: MH
authors: Cho
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000908:Antibodies, Fungal / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D005670:Fusarium / Q000276:immunology; D007093:Imidazoles / Q000737:chemistry; D008280:Magnetics / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010047:Ovalbumin / Q000737:chemistry; D014255:Trichothecenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D007093:Imidazoles; D009183:Mycotoxins; D014255:Trichothecenes; C006900:N,N-carbonyldiimidazole; D010047:Ovalbumin; C007262:deoxynivalenol
keywords: ELISA; deoxynivalenol; magnetic nanoparticles; monoclonal antibody
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