title: Salivary gland expression level of IκBα regulatory protein in Sjögren's syndrome.
abstract: Diagnosis and therapeutic strategies in Sjögren's syndrome (SS) might greatly benefit of the present multidisciplinary approach to studying the molecular pathogenesis of the disease. A deregulated inflammatory response has been described in the SS. The research in the last years sheds light on the importance of the NF-κB pathway regulating the pro-inflammatory cytokine production and leukocyte recruitment. These are important contributors to the inflammatory response during the development of SS. In this study we examine the expression of the NF-κB inhibitory protein termed IκBα in salivary glands epithelial cells (SGEC) comparing it with SGEC from healthy controls, to test the hypothesis that an altered expression of IκBα occurs in SGEC from SS biopsies. Real-Time PCR, western blot and immunohistochemistry demonstrated that the expression level of IκBα was significantly lower in SS with respect to healthy controls leading to an increased NF-κB activity. Our results suggest that the analysis of IκBα expression at salivary gland epithelial cell level could be a potential new hallmark of SS progression and sustain a rationale to more deeply investigate the therapeutic potential of specific NF-κB inhibitors in SS.
authors: Laboratory of Cell Biology, Section of Human Anatomy and Histology, Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Medical School, piazza Giulio Cesare 1, 70124, Bari, Italy. margherita.sisto@uniba.it
authors: Margherita
authors: M
authors: Sisto
authors: Sabrina
authors: S
authors: Lisi
authors: Dario Domenico
authors: DD
authors: Lofrumento
authors: Giuseppe
authors: G
authors: Ingravallo
authors: Raffaella
authors: R
authors: De Lucro
authors: Massimo
authors: M
authors: D'Amore
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha; D012469:Salivary Glands / Q000378:metabolism* / Q000473:pathology*; D012859:Sjogren's Syndrome / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
chemical_list: D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha
references: Autoimmunity. 1998;28(4):197-208
references: 9892501
references: Int Immunol. 2009 Dec;21(12):1341-9
references: 19854762
references: Int J Colorectal Dis. 2004 Mar;19(2):153-6
references: 13680285
references: Rheumatol Int. 2008 Jul;28(9):943-8
references: 18264709
references: Clin Immunol Immunopathol. 1994 Aug;72(2):162-5
references: 8050187
references: Clin Exp Med. 2011 Mar;11(1):1-10
references: 20454994
references: Chest. 2000 May;117(5):1482-7
references: 10807839
references: Immunobiology. 2010 Nov;215(11):874-83
references: 20061048
references: Exp Eye Res. 2009 Mar;88(3):398-409
references: 19103199
references: Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15193-8
references: 20696914
references: Genes Immun. 2010 Jul;11(5):432-8
references: 20535138
references: Cell. 1998 Dec 11;95(6):749-58
references: 9865693
references: Nat Rev Rheumatol. 2010 Sep;6(9):529-37
references: 20683439
references: Immunol Cell Biol. 2010 Mar-Apr;88(3):297-304
references: 19949420
references: Lab Invest. 1985 May;52(5):559-67
references: 2581066
references: Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8211-5
references: 9653166
references: Ann N Y Acad Sci. 2009 Aug;1171:407-14
references: 19723083
references: Lab Invest. 2012 Apr;92(4):615-24
references: 22157716
references: Ann N Y Acad Sci. 2007 Jun;1108:418-25
references: 17894006
references: Histochem Cell Biol. 2010 Oct;134(4):345-53
references: 20811902
references: N Engl J Med. 1997 Apr 10;336(15):1066-71
references: 9091804
references: Ann N Y Acad Sci. 2007 Mar;1098:15-21
references: 17332090
references: Genes Immun. 2002 Jun;3(4):211-9
references: 12058256
references: J Autoimmun. 2006 Aug;27(1):38-49
references: 16797160
references: Scand J Rheumatol. 2009 May-Jun;38(3):198-206
references: 19229767
references: Genes Immun. 2012 Jul;13(5):411-20
references: 22513453
references: Lab Invest. 1997 Sep;77(3):269-80
references: 9314950
references: Arthritis Res Ther. 2006;8(4):R86
references: 16684367
references: Pathology. 2012 Oct;44(6):557-61
references: 22935973
references: Histochem Cell Biol. 2013 Feb;139(2):371-9
references: 23052840
references: Clin Exp Med. 2010 Sep;10(3):199-203
references: 20033750
references: Clin Exp Immunol. 2001 Apr;124(1):126-33
references: 11359451
references: J Invest Dermatol. 2004 Mar;122(3):691-8
references: 15086555
references: Histochem Cell Biol. 2011 Jun;135(6):615-25
references: 21604024
references: Histochem Cell Biol. 2012 May;137(5):669-77
references: 22237885
references: Adv Physiol Educ. 2006 Dec;30(4):152-8
references: 17108242
references: J Autoimmun. 2010 Sep;35(2):160-70
references: 20673622
references: J Cutan Med Surg. 2011 Jan-Feb;15(1):8-14
references: 21291650
references: Ann Rheum Dis. 2002 Jun;61(6):554-8
references: 12006334
references: Curr Biol. 1998 Jan 1;8(1):R19-22
references: 9427616
references: J Clin Immunol. 2008 Sep;28(5):440-4
references: 18600435
references: Mol Cell Biol. 1996 May;16(5):2341-9
references: 8628301
references: Curr Mol Med. 2001 Jul;1(3):287-96
references: 11899077
references: Int J Immunopathol Pharmacol. 2008 Oct-Dec;21(4):1045-7
references: 19144293
references: Int Immunol. 2009 Mar;21(3):303-11
references: 19190085
references: Nat Rev Immunol. 2002 Oct;2(10):725-34
references: 12360211
references: Clin Rev Allergy Immunol. 2007 Jun;32(3):225-30
references: 17992589
references: Annu Rev Immunol. 1996;14:649-83
references: 8717528
references: J Clin Immunol. 2008 May;28(3):207-13
references: 18071880
references: J Clin Invest. 2001 Jan;107(1):7-11
references: 11134171
references: Mol Cell Biol. 1993 Jun;13(6):3301-10
references: 8497253
references: J Oral Pathol Med. 2007 Oct;36(9):511-23
references: 17850433