title: Effects of sevoflurane and propofol on cultured bone-marrow mesenchymal stem cells of rats.
abstract: OBJECTIVE
  Bone marrow mesenchymal stem cell (BMSC) is a potentially effective vehicle for the cell and gene therapy in clinical disease treatment. We studied whether the most commonly used anesthetic drugs have negative effects on rat BMSCs in vitro.
  
  
  METHODS
  The cultured BMSCs were treated with sevoflurane (in 1.7%, 2.3%, and 3%); propofol (5 μg/ml, 10 μg/ml and 20 μg/ml); or 2.3% sevoflurane plus 10 μg/ml propofol. After 4-hour treatment, the cultured BMSCs were prepared for MTT reduction assays and cell morphology observation.
  
  
  RESULTS
  Compared to the controls, the 4-hour sevoflurane exposure resulted in decreased cell viability of BMSCs in a concentration-dependent manner; however, 1.7% sevoflurane did not reduce the cell viability. The 4-hour propofol treatment did not affect the cell viability; but combined usage of 2.3% sevoflurane and 10 μg/ml propofol decreased cell viability. In BMSCs treated with higher concentration of sevoflurane (1.7% and 2.3%) and combined usage of the two anesthetics, the cell became raritas with wizened cytoplasm and had fewer connections to each other of BMSCs. More than 2.3%, or 2.3% sevoflurane plus 10 μg/ ml propofol caused cytotoxicity to BMSCs. However, propofol up to 20 μg/ml did not harm the BMSCs.
  
  
  CONCLUSIONS
  The study indicates that it is necessary to choose the right anesthesia during the BMSCs transplantation therapy.
authors: Department of Anesthesiology, The First Affiliated Hospital of SunYat-Sen University, Guangzhou, Guangdong, China.
authors: Xue
authors: X
authors: Zhou
authors: Ying-Qin
authors: YQ
authors: Li
authors: Wen
authors: W
authors: He
authors: Xiao-Yu
authors: XY
authors: Yang
authors: Fa-Huan
authors: FH
authors: Song
authors: Zhi-Bin
authors: ZB
authors: Zhou
authors: Ying
authors: Y
authors: Tang
authors: Xia
authors: X
authors: Feng
authors: Li-Hua
authors: LH
authors: Zhou
mesh_terms: D019162:Anesthetics, Combined / Q000008:administration & dosage / Q000633:toxicity*; D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000633:toxicity; D018686:Anesthetics, Intravenous / Q000008:administration & dosage / Q000633:toxicity; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D008738:Methyl Ethers / Q000008:administration & dosage / Q000633:toxicity*; D015742:Propofol / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane
chemical_list: D019162:Anesthetics, Combined; D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D008738:Methyl Ethers; D000077149:Sevoflurane; D015742:Propofol