title: C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches.
abstract: The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.
authors: Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors: Kana
authors: K
authors: Tozaki
authors: Junpei
authors: J
authors: Kimura
authors: Masahiro
authors: M
authors: Yasuda
authors: Nobuyuki
authors: N
authors: Ryu
authors: Testuo
authors: T
authors: Nasu
authors: Anton
authors: A
authors: Pernthaner
authors: Wayne R
authors: WR
authors: Hein
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D005333:Fetus; D006825:Hybridomas; D007082:Ileum / Q000648:ultrastructure*; D007413:Intestinal Mucosa / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal
keywords: Peyer’s patch; bovine; follicle-associated epithelium; monoclonal antibody
references: J Gen Virol. 2000 Sep;81(Pt 9):2327-2337
references: 10950992
references: Histochem J. 1992 Jan;24(1):33-9
references: 1372597
references: Anat Histol Embryol. 2004 Jun;33(3):172-9
references: 15144287
references: Res Vet Sci. 2007 Apr;82(2):141-9
references: 16949627
references: Cell Tissue Res. 1994 May;276(2):213-21
references: 7517344
references: J Anat. 1989 Apr;163:7-16
references: 2606783
references: Gastroenterology. 2005 Jul;129(1):321-7
references: 16012957
references: Anat Rec. 2002 Apr 1;266(4):207-17
references: 11920383
references: J Exp Med. 1994 Jul 1;180(1):15-23
references: 8006579
references: Cell Tissue Res. 1992 Aug;269(2):331-40
references: 1384978
references: Dev Comp Immunol. 2004 Apr;28(4):357-69
references: 14698221
references: Cell Tissue Res. 2005 Mar;319(3):395-404
references: 15657771
references: Science. 1997 Aug 15;277(5328):949-52
references: 9252325
references: Eur J Immunol. 1999 Feb;29(2):633-42
references: 10064080
references: Cell Tissue Res. 1993 Jul;273(1):127-36
references: 8364955
references: Arch Med Res. 2002 Jul-Aug;33(4):405-12
references: 12234531
references: Gastroenterology. 2001 Apr;120(5):1173-82
references: 11266381
references: Dev Immunol. 1998;6(3-4):223-31
references: 9814596
references: Immunol Cell Biol. 1988 Aug;66 ( Pt 4):261-8
references: 3154920
references: Immunol Rev. 2005 Aug;206:177-89
references: 16048549
references: J Immunol. 2003 Sep 15;171(6):2797-803
references: 12960300
references: Eur J Cell Biol. 1996 Dec;71(4):363-70
references: 8980907
references: Immunol Cell Biol. 1987 Jun;65 ( Pt 3):251-61
references: 3623609
references: Nat Med. 2001 Sep;7(9):976-7
references: 11533681
references: Immunol Rev. 2003 Oct;195:178-89
references: 12969318
references: Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6110-5
references: 15071180
references: Vet Pathol. 1991 Jan;28(1):22-9
references: 2017824
references: Gut. 2001 Apr;48(4):443-7
references: 11247881
references: Gastroenterology. 2004 Jul;127(1):213-23
references: 15236187
references: J Histochem Cytochem. 1993 Nov;41(11):1679-87
references: 7691933
references: J Acquir Immune Defic Syndr (1988). 1991;4(8):760-5
references: 1856788