title: Construction of a bovine enterovirus-based vector expressing a foot-and-mouth disease virus epitope.
abstract: A recombinant infectious bovine enterovirus (BEV) vector was constructed to express a foot-and-mouth disease virus (FMDV) capsid protein (VP1) epitope. Sequences encoding the VP1 epitope (amino acid residues 141-160) of FMDV (vaccine strain O1/Manisa/Turkey/69) were inserted into pBLUBEV at the VP1/2A junction. The growth characteristics of the parental virus and viruses derived from recombinant plasmids (pBLUBEV, pBLUBEV-Manisa-epi) were determined by plaque assay and one-step growth curve analysis. There were no significant differences in the growth kinetics and plaque morphologies between transfectant viruses and their parental virus. The expressed VP1 epitope was detected successfully by using indirect immunofluorescence assay with a polyclonal antibody against the FMDV VP1 epitope from Madin Darby bovine kidney (MDBK) cells infected with BEV-Manisa-epi transfectant virus. This study demonstrated a novel alternative live viral vector that may be utilized as a candidate vaccine vector for veterinary applications.
authors: Clinical Research Center of the Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors: Jia-Qi
authors: JQ
authors: Chu
authors: Yeo-Joo
authors: YJ
authors: Lee
authors: Jeong-Nam
authors: JN
authors: Park
authors: Su-Mi
authors: SM
authors: Kim
authors: Kwang-Nyeong
authors: KN
authors: Lee
authors: Young-Joon
authors: YJ
authors: Ko
authors: Hyang-Sim
authors: HS
authors: Lee
authors: In-Soo
authors: IS
authors: Cho
authors: Byounghan
authors: B
authors: Kim
authors: Jong-Hyeon
authors: JH
authors: Park
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology*; D002417:Cattle; D004285:Dogs; D029650:Enterovirus, Bovine / Q000235:genetics* / Q000254:growth & development; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary*; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors; D061985:Madin Darby Canine Kidney Cells; D012367:RNA, Viral / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D012367:RNA, Viral; C442501:VP1 protein, Foot-and-mouth disease virus; D014765:Viral Vaccines