title: Suppression of chemically induced and spontaneous mouse oocyte activation by AMP-activated protein kinase.
abstract: Oocyte activation is an important process triggered by fertilization that initiates embryonic development. However, parthenogenetic activation can occur either spontaneously or with chemical treatments. The LT/Sv mouse strain is genetically predisposed to spontaneous activation. LT oocytes have a cell cycle defect and are ovulated at the metaphase I stage instead of metaphase II. A thorough understanding of the female meiosis defects in this strain remains elusive. We have reported that AMP-activated protein kinase (PRKA) has an important role in stimulating meiotic resumption and promoting completion of meiosis I while suppressing premature parthenogenetic activation. Here we show that early activation of PRKA during the oocyte maturation period blocked chemically induced activation in B6SJL oocytes and spontaneous activation in LT/SvEiJ oocytes. This inhibitory effect was associated with high levels of MAPK1/3 activity. Furthermore, stimulation of PRKA partially rescued the meiotic defects of LT/Sv mouse oocytes in concert with correction of abnormal spindle pole localization of PRKA and loss of prolonged spindle assembly checkpoint activity. Altogether, these results confirm a role for PRKA in helping sustain the MII arrest in mature oocytes and suggest that dysfunctional PRKA contributes to meiotic defects in LT/SvEiJ oocytes.
authors: Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA.
authors: Ru
authors: R
authors: Ya
authors: Stephen M
authors: SM
authors: Downs
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives; D000818:Animals; D000001:Calcimycin; D018797:Cell Cycle Proteins / Q000378:metabolism; D004789:Enzyme Activation; D000431:Ethanol; D005260:Female; D064246:Mad2 Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology*; D012265:Ribonucleotides
chemical_list: D018797:Cell Cycle Proteins; D064246:Mad2 Proteins; C577698:Mad2l1 protein, mouse; D012265:Ribonucleotides; D000620:Aminoimidazole Carboxamide; D000001:Calcimycin; D000431:Ethanol; D055372:AMP-Activated Protein Kinases; C031143:AICA ribonucleotide
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