title: [Content determination of two isomers containd in Garcinia hanburyi by HPLC].
abstract: OBJECTIVE
  To establish a method for determing the content of two isomers containd in Garcinia hanburyi by HPLC.
  
  
  METHODS
  Chromatographic column of SunFire (Waters) C8 (2.1 mm x 150 mm, 3.5 microm) was adopted, with acetonitrile-methanol-0.3% trifluoroacetic acid (36: 37:27) as the mobile phase. The detection wavelength was 360 nm,the flow rate was 0.3 mL x min(-1), and the column temperature was 28 degrees C.
  
  
  RESULTS
  The linear regression equation of r-gambogic acid was Y = 2.87 x 10(6) X - 2.24 x 10(5), r = 0.999 9. The linear regression equation of S-gambogic acid was Y = 3.31 x 10(6) X - 1.44 x 10(5), r = 0.999 9. The average recoveries were 100.0% and 100.9%, with RSD being 2.1% and 2.5% (n = 6), respectivley. The average contents of two gambogic acid in G. hanburyi were 30.06% and 21.45%, respectively.
  
  
  CONCLUSIONS
  The method was so convenient and stable that it can be used for identification and content determination of two isomers containd in G. hanburyi.
authors: Tianjin Key Laboratory of Molecular Design and Drug Discovery, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China. zjy88521@126.com
authors: Jun-Yan
authors: JY
authors: Zhang
authors: Guang-Ping
authors: GP
authors: Xia
authors: Na-Xia
authors: NX
authors: Zhao
authors: Yun-Ping
authors: YP
authors: Chang
authors: Ying-Mei
authors: YM
authors: Han
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029760:Garcinia / Q000737:chemistry*; D007536:Isomerism; D016014:Linear Models; D044004:Xanthones / Q000032:analysis*
chemical_list: D044004:Xanthones; C052659:gambogic acid