title: Case-control study of a multistate equine herpesvirus myeloencephalopathy outbreak.
abstract: BACKGROUND
  A large multistate outbreak of equine herpesvirus myeloencephalopathy (EHM) occurred in May 2011 among horses that participated in a competitive event.
  
  
  OBJECTIVE
  To identify EHM risk factors among horses with a common exposure venue.
  
  
  METHODS
  A total of 123 horses: 19 horses with EHM, 14 equine herpesvirus-1 cases with no reported neurologic signs, and 90 control horses.
  
  
  METHODS
  EHM case survey data were compared with data from EHV-1 cases with no neurologic signs and healthy controls using univariable and multivariable methods.
  
  
  RESULTS
  Significant factors associated with higher risk for EHM compared with EHV-1 cases with no neurologic signs were (1) greater number of biosecurity risks at the event, (2) female sex, (3) increasing number of classes competed in at the event, and (4) an interaction between sex and number of classes competed in. In the EHM versus controls comparison, in addition to sex and biosecurity risks, factors associated with higher EHM risk included EHV-1 vaccination in the 5 weeks before the event and increasing number of events attended in April 2011; zinc dietary supplementation was associated with decreased risk. An interaction between sex and the number of events attended in April 2011 also was significant.
  
  
  CONCLUSIONS
  Findings from this study suggest that dietary zinc supplementation may be associated with decreased risk of EHM. Several factors were associated with increased risk of EHM. Additional investigations of factors associated with risk of EHM are warranted to evaluate the importance of these factors in this complex disease of horses.
authors: Colorado State University College of Veterinary Medicine and Biomedical Sciences, Animal Population Health Institute (APHI), Fort Collins, CO 80523, USA. jtraub1@lamar.colostate.edu
authors: J L
authors: JL
authors: Traub-Dargatz
authors: A M
authors: AM
authors: Pelzel-McCluskey
authors: L H
authors: LH
authors: Creekmore
authors: S
authors: S
authors: Geiser-Novotny
authors: T R
authors: TR
authors: Kasari
authors: A M
authors: AM
authors: Wiedenheft
authors: E J
authors: EJ
authors: Bush
authors: K E
authors: KE
authors: Bjork
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D004196:Disease Outbreaks / Q000662:veterinary*; D004679:Encephalomyelitis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D005260:Female; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification*; D006734:Horse Diseases / Q000453:epidemiology* / Q000821:virology*; D006736:Horses; D008297:Male; D015999:Multivariate Analysis; D012307:Risk Factors; D011795:Surveys and Questionnaires; D014481:United States / Q000453:epidemiology