title: Calcium alginate beads encapsulated PMMA-g-CS nano-particles for α-chymotrypsin immobilization.
abstract: Chitosan grafted with polymethyl methacrylate (PMMA-g-CS) was prepared via a free-radicals polymerization technique as a carrier for enzyme immobilization. α-Chymotrypsin (CT), as an enzyme model in this study, was immobilized onto the prepared PMMA-g-CS via covalent bonding. Calcium alginate (CA) beads were developed for encapsulating PMMA-g-CS-CT to produce PMMA-g-CS-CT/CA composite beads. Morphology and size of PMMA-g-CS particles were investigated by TEM and found to be in the nanoscale. The structure and surface morphology of the beads before and after immobilization process were characterized by FT-IR and SEM, respectively. Both the bound CT content and relative activity of immobilized enzyme were measured. A higher retained activity (about 97.7%) obtained for the immobilized CT at pH 9 for 24 h. The results indicated that immobilized CT maintained excellent performance even after 25 reuses and retained 75% from its original activity after 60 days of storage at 25 °C.
authors: Polymers and Pigments Department, National Research Center, Dokki, Giza, Egypt. mghaffar50@yahoo.com
authors: M A
authors: MA
authors: Abd El-Ghaffar
authors: M S
authors: MS
authors: Hashem
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002214:Capsules; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry*; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D000464:Alginates; D002214:Capsules; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D019904:Polymethyl Methacrylate; D048271:Chitosan; D002918:Chymotrypsin; C117608:alpha-chymotrypsin