title: Novel registration for microcomputed tomography and bioluminescence imaging based on iterated optimal projection.
abstract: As a high-sensitivity imaging modality, bioluminescence tomography can reconstruct the three-dimensional (3-D) location of an internal luminescent source based on the 3-D surface light distribution. However, we can only get the multi-orientation two-dimensional (2-D) bioluminescence distribution in the experiments. Therefore, developing an accurate universal registration method is essential for following bioluminescent source reconstruction. We can then map the multi-orientation 2-D bioluminescence distribution to the 3-D surface derived from anatomical information with it. We propose a 2-D -to-3-D registration method based on iterated optimal projection and applied it in a registration and reconstruction study of three transgenic mice. Compared with traditional registration methods based on the fixed points, our method was independent of the markers and the registration accuracy of the three experiments was improved by 0.3, 0.5, and 0.4 pixels, respectively. In addition, based on the above two registration results using the two registration methods, we reconstructed the 3-D location of the inner bioluminescent source in the three transgenic mice. The reconstruction results showed that the average error distance between the center of the reconstructed element and the center of the real element were reduced by 0.32, 0.48, and 0.39 mm, respectively.
authors: Chinese Academy of Sciences, Intelligent Medical Research Center, Institute of Automation, Beijing 100190, China.
authors: Xibo
authors: X
authors: Ma
authors: Kexin
authors: K
authors: Deng
authors: Zhenwen
authors: Z
authors: Xue
authors: Xueyan
authors: X
authors: Liu
authors: Shouping
authors: S
authors: Zhu
authors: Chenghu
authors: C
authors: Qin
authors: Xin
authors: X
authors: Yang
authors: Jie
authors: J
authors: Tian
mesh_terms: D000465:Algorithms; D000818:Animals; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000706:statistics & numerical data; D051379:Mice; D008822:Mice, Transgenic; D061848:Optical Imaging / Q000379:methods* / Q000706:statistics & numerical data; D055620:Optical Phenomena; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D011857:Radiographic Image Interpretation, Computer-Assisted; D055114:X-Ray Microtomography / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D015675:Osteocalcin; D008156:Luciferases