title: Vogesella fluminis sp. nov., isolated from a freshwater river, and emended description of the genus Vogesella.
abstract: A bacterial strain designated Npb-07(T) was isolated from a freshwater river in Taiwan and characterized using a polyphasic taxonomic approach. Strain Npb-07(T) was Gram-negative, aerobic, rod-shaped and motile by means of a single polar flagellum. Growth occurred at 10-37 °C (optimum, 20-30 °C), at pH 6.0-9.0 (optimum, pH 6.0-7.0) and with 0-1 % NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-07(T) belonged to the genus Vogesella and its most closely related neighbour was Vogesella indigofera ATCC 19706(T) with sequence similarity of 98.4 %. The major fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 44.4 %) and C16 : 0 (31.9 %). The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 65.3 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two uncharacterized aminophospholipids and an uncharacterized phospholipid. The DNA-DNA relatedness of strain Npb-07(T) with respect to V. indigofera ATCC 19706(T) was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Npb-07(T) represents a novel species in the genus Vogesella, for which the name Vogesella fluminis sp. nov. is proposed. The type strain is Npb-07(T) ( = LMG 26669(T) = BCRC 80377(T) = KCTC 23713(T)).
authors: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
authors: Shih-Yi
authors: SY
authors: Sheu
authors: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
authors: Jhen-Ci
authors: JC
authors: Chen
authors: College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan.
authors: Chiu-Chung
authors: CC
authors: Young
authors: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
authors: Wen-Ming
authors: WM
authors: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S