title: Detection of chitinase ChiA produced by Serratia marcescens PRC-5, using anti-PrGV-chitinase.
abstract: In this study, a bacterium Serratia marcescens PRC-5 that displayed strong chitinolytic activity on 0.5% colloidal chitin-containing agar medium was isolated from soil. The chitinase activity increased rapidly with a maximum level (6.14 U/mL) on 4 days of incubation with swollen chitin (pH 5.0). Three active bands of chitinase isozymes were observed (53, 44, and 34 kDa) on SDS-PAGE gel and there pI values ranged from pI 5.4 to 5.8 on 2D gels. The chitinase from the PRC-5 strain was also able to produce GlcNAc monomers on TLC plates. The chitinase of PRC-5 inhibited the mycelial growth of Rhizoctonia solani KACC40111, which indicates that it could be used as a biocontrol agent for phytophathogens. The chitinase isozyme N1, which had a molecular weight of 62 kDa, was transferred from a native and SDS-PAGE gel onto an immunoblot and was probed using an anti-PrGV-chitinase.
authors: Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture (IEFA), Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Yong-Su
authors: YS
authors: Song
authors: Seunghan
authors: S
authors: Oh
authors: Yeon-Soo
authors: YS
authors: Han
authors: Dong-Jun
authors: DJ
authors: Seo
authors: Ro-Dong
authors: RD
authors: Park
authors: Woo-Jin
authors: WJ
authors: Jung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002688:Chitinases / Q000032:analysis* / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D008969:Molecular Sequence Data; D051381:Rats; D012232:Rhizoctonia / Q000254:growth & development; D012706:Serratia marcescens / Q000378:metabolism* / Q000502:physiology; D012988:Soil Microbiology
chemical_list: D007074:Immunoglobulin G; D002688:Chitinases