title: KCTD9 contributes to liver injury through NK cell activation during hepatitis B virus-induced acute-on-chronic liver failure.
abstract: We explored the expression of a newly identified potassium channel tetramerisation domain containing 9 (KCTD9) protein in 113 blood and 81 liver samples, from patients with mild chronic hepatitis B (CHB) or HBV-induced acute-on-chronic liver failure (HBV-ACLF). KCTD9 was highly expressed in peripheral and hepatic NK cells from HBV-ACLF patients compared with mild CHB patients, and this correlated positively with the severity of liver injury. The role of KCTD9 was further investigated in NK92 cells in vitro. KCTD9 overexpressed NK92 cells exhibited a marked increase in CD69 expression, cytotoxicity, IFN-γ secretion and a significant decrease in NKG2A receptor expression. Inhibition of KCTD9 by shRNA resulted in reduced cytotoxic function. These results suggest the involvement of KCTD9 in NK cell activation and provide additional insight into a potential therapeutic target for molecular manipulation for HBV-ACLF patients.
authors: Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors: Tao
authors: T
authors: Chen
authors: Lin
authors: L
authors: Zhu
authors: Yaoyong
authors: Y
authors: Zhou
authors: Bin
authors: B
authors: Pi
authors: Xiaojuan
authors: X
authors: Liu
authors: Guohong
authors: G
authors: Deng
authors: Rong
authors: R
authors: Zhang
authors: Yuming
authors: Y
authors: Wang
authors: Zeguang
authors: Z
authors: Wu
authors: Meifang
authors: M
authors: Han
authors: Xiaoping
authors: X
authors: Luo
authors: Qin
authors: Q
authors: Ning
mesh_terms: D000293:Adolescent; D000328:Adult; D002460:Cell Line; D002648:Child; D058625:End Stage Liver Disease / Q000276:immunology*; D005260:Female; D020869:Gene Expression Profiling; D056945:Hep G2 Cells; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D017114:Liver Failure, Acute / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D015221:Potassium Channels / Q000235:genetics / Q000276:immunology*; D055815:Young Adult
chemical_list: C580589:KCTD9 protein, human; D015221:Potassium Channels