title: Multimodal examination of structural and functional remapping in the mouse photothrombotic stroke model.
abstract: Recent studies show a limited capacity for neural repair after stroke, which includes remapping of sensorimotor functions and sprouting of new connections. However, physiologic and connectional plasticity of sensory maps during long-term functional recovery in the mouse have not been determined. Using a photothrombotic stroke model, we targeted the motor cortex, which we show results in lasting behavioral deficits on the grid-walking and in the cylinder tasks out to 8 weeks after stroke. Mice recovered performance in a skilled reaching task, showing no deficit from week 2 after stroke. Long-term optical intrinsic signal imaging revealed functional reorganization of sensory cortical maps for both forelimb and hindlimb, with more diffuse sensory physiologic maps. There was a small but significant increase in motor neuron projections within the areas of functional cortical reorganization as assessed using the neuroanatomic tracer biotinylated dextran amine. These findings show that the sensorimotor cortex undergoes remapping of cortical functions and axonal sprouting within the same regions during recovery after stroke. This suggests a linked structural and physiologic plasticity underlying recovery. Combined long-term structural and functional mapping after stroke in the mouse is practical and provides a rich data set for mechanistic analysis of stroke recovery.
authors: Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.
authors: Andrew N
authors: AN
authors: Clarkson
authors: Héctor E
authors: HE
authors: López-Valdés
authors: Justine J
authors: JJ
authors: Overman
authors: Andrew C
authors: AC
authors: Charles
authors: K C
authors: KC
authors: Brennan
authors: S
authors: S
authors: Thomas Carmichael
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology* / Q000528:radiation effects*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity; D009044:Motor Cortex / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D020521:Stroke / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D013927:Thrombosis / Q000150:complications* / Q000209:etiology; D016138:Walking
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