title: Analysis of myeloperoxidase activity in wound fluids as a marker of infection.
abstract: BACKGROUND
  Neutrophilic polymorphonuclear leukocytes play a crucial role in the host defence against bacterial and fungal infections. They participate in the inflammatory response through the liberation of peptides and enzymes like myeloperoxidase (MPO). Therefore, MPO has a potential as a marker enzyme for the diagnosis of wound infection.
  
  
  METHODS
  Substrate specificities and reaction pathways of MPO were investigated for new MPO substrates: crystal violet, leuco crystal violet, fast blue RR (4-benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt) and various systematically substituted model substrates based on 2,7-dihydroxy-1-(4-hydroxyphenylazo)naphtalene-3,6-disulphonic acid. In addition, fast blue RR was covalently bound to siloxanes allowing immobilization of the substrate, while cellobiosedehydrogenase was integrated for generation of hydrogen peroxide required by MPO.
  
  
  RESULTS
  Elevated concentrations of MPO were found in infected wounds compared with non-infected wounds (92.2 ± 45.0 versus 1.9 ± 1.8 U/mL). Various soluble and immobilized substrates were oxidized by MPO in wound samples and the influence of substrate structure and reaction pathways were elucidated for selected compounds.
  
  
  CONCLUSIONS
  Incubation of different MPO substrates with infected wound fluid samples resulted in a clear colour change in the case of elevated MPO concentrations, thus allowing early diagnosis of wound infection.
authors: Department of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, Graz, Austria.
authors: A
authors: A
authors: Hasmann
authors: E
authors: E
authors: Wehrschuetz-Sigl
authors: A
authors: A
authors: Marold
authors: H
authors: H
authors: Wiesbauer
authors: R
authors: R
authors: Schoeftner
authors: U
authors: U
authors: Gewessler
authors: A
authors: A
authors: Kandelbauer
authors: D
authors: D
authors: Schiffer
authors: K P
authors: KP
authors: Schneider
authors: B
authors: B
authors: Binder
authors: M
authors: M
authors: Schintler
authors: G M
authors: GM
authors: Guebitz
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001826:Body Fluids / Q000201:enzymology* / Q000378:metabolism; D057075:Enzyme Assays; D006801:Humans; D006861:Hydrogen Peroxide; D009504:Neutrophils / Q000201:enzymology* / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000032:analysis*; D014946:Wound Infection / Q000175:diagnosis* / Q000201:enzymology*
chemical_list: D015415:Biomarkers; D006861:Hydrogen Peroxide; D009195:Peroxidase