title: MicroRNA 181a influences the expression of HMGB1 and CD4 in acute Leukemias.
abstract: Dysregulation of microRNAs (miRs) has been linked to several types of cancer. In the present study, we investigated the expression of miR-181a in different leukemia cell lines and healthy hematopoietic cells, as well as its influence on cell proliferation, metabolic activity and potential targets. Expression of miR-181a differed between various leukemia cell lines and mature blood cells. Inhibition of miR 181a expression in T- and B-Acute Lymphoblastic Leukemia (ALL) cells revealed an influence on the potential targets High-Mobility-Group-Protein B1 (HMGB1) and Cluster of Differentiation 4 (CD4). Overexpression of miR-181a in AML cells led to a significant decrease in cell proliferation and metabolic activity. The present data indicate a possible role of this specific miRNA in immunogenicity.
authors: University of Rostock, Division of Medicine, Department of Hematology/Oncology/Palliative Medicine, Ernst-Heydemann-Str. 6, 18057 Rostock, Germany. christian.junghanss@med.uni-rostock.de
authors: Meike
authors: M
authors: Dahlhaus
authors: Catrin
authors: C
authors: Schult
authors: Sandra
authors: S
authors: Lange
authors: Mathias
authors: M
authors: Freund
authors: Christian
authors: C
authors: Junghanss
mesh_terms: D015153:Blotting, Western; D015704:CD4 Antigens / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002469:Cell Separation; D018274:Electroporation; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D024243:HMGB1 Protein / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D015704:CD4 Antigens; D024243:HMGB1 Protein; C540193:MIrn181 microRNA, human; D035683:MicroRNAs