title: Large gradient high magnetic fields affect osteoblast ultrastructure and function by disrupting collagen I or fibronectin/αβ1 integrin.
abstract: The superconducting magnet generates a field and field gradient product that can levitate diamagnetic materials. In this study a specially designed superconducting magnet with a large gradient high magnetic field (LG-HMF), which can provide three apparent gravity levels (μ-g, 1-g, and 2-g), was used to simulate a space-like gravity environment. The effects of LG-HMF on the ultrastructure and function of osteoblast-like cells (MG-63 and MC3T3-E1) and the underlying mechanism were investigated by transmission electromicroscopy (TEM), MTT, and cell western (ICW) assays. Under LG-HMF significant morphologic changes in osteoblast-like cells occurred, including expansion of endoplasmic reticulum and mitochondria, an increased number of lysosomes, distorted microvilli, and aggregates of actin filaments. Compared to controls, cell viability and alkaline phosphatase (ALP) secretion were significantly increased, and collagen I (col I), fibronectin (FN), vinculin, integrin α3, αv, and β1 expression were changed under LG-HMF conditions. In conclusion, LG-HMF affects osteoblast ultrastructure, cell viability, and ALP secretion, and the changes caused by LG-HMF may be related to disrupting col I or FN/αβ1 integrin.
authors: Key Laboratory for Space Biosciences & Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University Xi'an, Xi'an, China.
authors: Ai-Rong
authors: AR
authors: Qian
authors: Xiang
authors: X
authors: Gao
authors: Wei
authors: W
authors: Zhang
authors: Jing-Bao
authors: JB
authors: Li
authors: Yang
authors: Y
authors: Wang
authors: Sheng-Meng
authors: SM
authors: Di
authors: Li-Fang
authors: LF
authors: Hu
authors: Peng
authors: P
authors: Shang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D003094:Collagen / Q000378:metabolism; D003599:Cytoskeleton / Q000528:radiation effects; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006112:Gravitation; D016023:Integrins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism / Q000528:radiation effects; D060526:Magnetic Fields; D051379:Mice; D010006:Osteoblasts / Q000528:radiation effects* / Q000648:ultrastructure
chemical_list: D005353:Fibronectins; D016023:Integrins; D003094:Collagen; D000469:Alkaline Phosphatase
references: Clin Chem. 2000 Aug;46(8 Pt 1):1136-43
references: 10926894
references: Bone. 1998 May;22(5 Suppl):139S-143S
references: 9600771
references: J Biol Chem. 1997 Nov 14;272(46):29309-16
references: 9361011
references: Biophys J. 2002 Mar;82(3):1260-5
references: 11867443
references: J Bone Miner Res. 2002 Oct;17(10):1814-21
references: 12369785
references: Adv Space Res. 2002;30(4):765-70
references: 12528727
references: J Bone Miner Res. 2000 Jun;15(6):1099-112
references: 10841178
references: J Magn Reson Imaging. 2005 Sep;22(3):334-40
references: 16106367
references: J Cell Biochem. 2000 Sep 14;79(4):557-65
references: 10996846
references: FASEB J. 1999;13 Suppl:S121-7
references: 10352153
references: IEEE Trans Biomed Eng. 2012 Jan;59(1):68-77
references: 21216704
references: Prog Biophys Mol Biol. 2005 Feb-Apr;87(2-3):213-23
references: 15556660
references: Acta Biochim Biophys Sin (Shanghai). 2011 Feb;43(2):133-42
references: 21266543
references: Adv Space Res. 2005;36(1):114-8
references: 16252445
references: Brain Res Dev Brain Res. 2001 Oct 24;130(2):191-205
references: 11675122
references: J Bone Miner Res. 2004 Mar;19(3):436-46
references: 15040832
references: Nucleic Acids Res. 1999 Feb 15;27(4):1079-83
references: 9927742
references: Bioelectromagnetics. 2009 Oct;30(7):545-55
references: 19475564
references: Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13051-6
references: 16916937
references: J Bone Miner Res. 1990 Aug;5(8):831-42
references: 2239367
references: Nat Rev Mol Cell Biol. 2009 May;10(5):360-4
references: 19293819
references: J Exp Bot. 1997 Nov;48(316):1951-7
references: 11541075
references: Acta Biochim Biophys Sin (Shanghai). 2009 Jul;41(7):561-77
references: 19578720
references: Nat Protoc. 2007;2(10):2439-50
references: 17947985
references: Adv Space Res. 2001;27(5):1001-5
references: 11596629
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: Curr Opin Cell Biol. 1991 Oct;3(5):841-8
references: 1931084
references: Anal Biochem. 2010 Mar 15;398(2):254-6
references: 20018162
references: J Cell Biochem. 2004 Nov 1;93(4):697-707
references: 15660414
references: Stem Cell Rev Rep. 2010 Dec;6(4):567-78
references: 20697977
references: Biophys J. 1997 Aug;73(2):1130-3
references: 9251829
references: J Dent Res. 2008 Jul;87(7):650-4
references: 18573985
references: Planta. 1996;198(1):87-94
references: 8580774
references: Microgravity Sci Technol. 2009 Nov;21(4):311-318
references: 20052306
references: Int J Radiat Biol. 2012 Nov;88(11):806-13
references: 22642494
references: Bone. 1997 Jun;20(6):547-51
references: 9177869