title: Altered functions of natural killer cells in response to L-Arginine availability.
abstract: L-Arginine (L-Arg) availability is crucial in the regulation of immune response. Indeed, L-Arg deficiency induces T-cell dysfunction and could modulate the properties of natural killer (NK) cells involved in the early host defense against infections and tumors. We explored the impact of L-Arg depletion on NK cell functions using two models - an NK-92 cell line and isolated human blood NK cells. Below 5mg/L of L-Arg, NK-92 cell proliferation was decreased and a total L-Arg depletion reduced NK-92 cell viability. NK cell cytotoxicity was significantly inhibited in presence of low L-Arg concentration (2.5 mg/L). L-Arg depletion reduced the expression of NK-92 activating receptors, NKp46 and NKp30, the expression of NK ζ chain and the NK-92 intracellular production of IFN-γ. Whatever the L-Arg concentrations tested, no significant variation in the gene expression of transporters and enzymes involved in L-Arg metabolism was found. Thus, L-Arg availability modulates the phenotypic and functional properties of NK cells.
authors: Clermont Université, Université d'Auvergne, EA 4233, Nutrition Cancerogenèse et Thérapie anti-tumorale, CLARA, CRNH Auvergne, Clermont-Ferrand, France. bruno.lamas@ext.udamail.fr
authors: Bruno
authors: B
authors: Lamas
authors: Juliette
authors: J
authors: Vergnaud-Gauduchon
authors: Nicolas
authors: N
authors: Goncalves-Mendes
authors: Olivier
authors: O
authors: Perche
authors: Adrien
authors: A
authors: Rossary
authors: Marie-Paule
authors: MP
authors: Vasson
authors: Marie-Chantal
authors: MC
authors: Farges
mesh_terms: D001120:Arginine / Q000502:physiology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000032:analysis; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000032:analysis
chemical_list: C112260:NCR1 protein, human; C431710:NCR3 protein, human; D055627:Natural Cytotoxicity Triggering Receptor 1; D055628:Natural Cytotoxicity Triggering Receptor 3; D007371:Interferon-gamma; D001120:Arginine