title: [Expression  and clinicopathological significance of  GRAMD4  in hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the expression of GRAMD4 and its correlation with the clinicopathological features in hepatocellular carcinoma (HCC).
  
  
  METHODS
  We freshly collected 40 samples of surgically resected paired HCC and normal tumor-adjacent tissues and detected the GRAMD4 expression in the samples using RT-PCR and immunohistochemical staining. The correlation between GRAMD4 mRNA expression and clinicopathological features was analyzed. The GRAMD4 mRNA expression in normal liver cell line (LO2) and HCC cell lines (HepG2, Hep3B and SMMC-7721) was detected with RT-PCR.
  
  
  RESULTS
  The mRNA and protein expressions of GRAMD4 in HCC tissues were significantly upregulated compared to those of the normal tumor-adjacent tissues (P<0.05, respectively). Clinicopathological analysis suggested that GRAMD4 mRNA expression was positively associated with tumor size, Edmonson-Steiner classification and advanced TNM stage (P<0.05). Expression of GRAMD4 mRNA in HCC cell lines HepG2, Hep3B, and SMMC-7721 was significantly higher than that in L02 (P<0.05, respectively).
  
  
  CONCLUSIONS
  The expression of GRAMD4 is up-regulated in HCC cell lines and HCC tissues, and the increased expression is correlated with the clinicopathological characteristics of HCC.
authors: Department of Hepatobiliary Surgery, Xi'an Jiaotong University, China.
authors: Jing
authors: J
authors: Zhang
authors: Kangsheng
authors: K
authors: Tu
authors: Zhenyu
authors: Z
authors: Zhou
authors: Xin
authors: X
authors: Zheng
authors: Jie
authors: J
authors: Gao
authors: Yingmin
authors: Y
authors: Yao
authors: Qingguang
authors: Q
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
chemical_list: C503369:GRAMD4 protein, human; D024101:Mitochondrial Proteins; D012333:RNA, Messenger