title: Ligand binding to anion-binding exosites regulates conformational properties of thrombin.
abstract: Thrombin participates in coagulation, anticoagulation, and initiation of platelet activation. To fulfill its diverse roles and maintain hemostasis, this serine protease is regulated via the extended active site region and anion-binding exosites (ABEs) I and II. For the current project, amide proton hydrogen-deuterium exchange coupled with MALDI-TOF mass spectrometry was used to characterize ligand binding to individual exosites and to investigate the presence of exosite-active site and exosite-exosite interactions. PAR3(44-56) and PAR1(49-62) were observed to bind to thrombin ABE I and then to exhibit long range effects over to ABE II. By contrast, Hirudin(54-65) focused more on ABE I and did not transmit influences over to ABE II. Although these three ligands were each directed to ABE I, they did not promote the same conformational consequences. D-Phe-Pro-Arg-chloromethyl ketone inhibition at the thrombin active site led to further local and long range consequences to thrombin-ABE I ligand complexes with the autolysis loop often most affected. When Hirudin(54-65) was bound to ABE I, it was still possible to bind GpIbα(269-286) or fibrinogen γ'(410-427) to ABE II. Each ligand exerted its predominant influences on thrombin and also allowed interexosite communication. The results obtained support the proposal that thrombin is a highly dynamic protein. The transmission of ligand-specific local and long range conformational events is proposed to help regulate this multifunctional enzyme.
authors: Chemistry Department, University of Louisville, Louisville, Kentucky 40292.
authors: Marina V
authors: MV
authors: Malovichko
authors: Chemistry Department, University of Louisville, Louisville, Kentucky 40292.
authors: T Michael
authors: TM
authors: Sabo
authors: Chemistry Department, University of Louisville, Louisville, Kentucky 40292. Electronic address: muriel.maurer@louisville.edu.
authors: Muriel C
authors: MC
authors: Maurer
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000737:chemistry; D000818:Animals; D000838:Anions / Q000737:chemistry; D001665:Binding Sites; D020134:Catalytic Domain; D002417:Cattle; D041961:Deuterium Exchange Measurement; D005340:Fibrinogen / Q000737:chemistry; D006629:Hirudins / Q000737:chemistry*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry*; D019038:Platelet Glycoprotein GPIb-IX Complex; D011485:Protein Binding; D018179:Receptors, Thrombin / Q000737:chemistry; D013917:Thrombin / Q000737:chemistry*
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D000838:Anions; D006629:Hirudins; D008024:Ligands; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D019038:Platelet Glycoprotein GPIb-IX Complex; D018179:Receptors, Thrombin; C094990:adhesion receptor; C091596:hirudin (54-65); D005340:Fibrinogen; D013917:Thrombin; C040308:phenylalanyl-prolyl-arginine-chloromethyl ketone
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