title: Adaptation of Cupriavidus necator to conditions favoring polyhydroxyalkanoate production.
abstract: The fatty acid (FA) composition of the bacterial membrane of Cupriavidus necator DSM 545 was assessed during the time course of two-stage fed-batch cultivations for the production of short-chain polyhydroxyalkanoates (PHA). Changes in the relative proportion of straight, methyl and cyclopropyl saturated, unsaturated, hydroxy substituted and polyunsaturated FA were observed, depending on the C sources and cultivation conditions used to favor the synthesis of poly(3-hydroxybutyrate) (P(3HB)), poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)) or poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) (P(3HB-4HB-3HV)), under N limiting conditions. The relative percentage of each FA class was studied using glucose or waste glycerol (GRP), as main C source for P(3HB) production. The FA profile was also assessed when GRP was used together with i) γ-butyrolactone (GBL) (precursor of 4HB monomers) for P(3HB-4HB) synthesis and ii) GBL and propionic acid (PA) (3HV precursor) to yield P(3HB-4HB-3HV). The effect of GBL and PA utilization as PHA monomer precursors on the FA profile of the cell membrane was studied under two different dissolved oxygen concentrations (DOC).
authors: Department of Bioengineering, Universidade Técnica de Lisboa, Lisboa, Portugal.
authors: João M B T
authors: JM
authors: Cavalheiro
authors: M Catarina M D
authors: MC
authors: de Almeida
authors: M Manuela R
authors: MM
authors: da Fonseca
authors: Carla C C R
authors: CC
authors: de Carvalho
mesh_terms: D000818:Animals; D001709:Biotechnology; D018929:Cell Culture Techniques / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D048429:Cell Size; D002478:Cells, Cultured; D020118:Cupriavidus necator / Q000166:cytology / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol; D016018:Least-Squares Analysis; D054813:Polyhydroxyalkanoates / Q000032:analysis / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D054813:Polyhydroxyalkanoates; D005990:Glycerol