title: Efficient cryopreservation protocol enables accessibility of a broad range of ammonia-oxidizing bacteria for the scientific community.
abstract: Long-term storage of the fastidious ammonia-oxidizing bacteria has proven difficult, which limits their public availability and results in a loss of cultured biodiversity. To enable their accessibility to the scientific community, an effective protocol for cryopreservation of ammonia-oxidizing cultures at -80 °C and in liquid nitrogen was developed. Long-term storage could be achieved using 5% DMSO as cryoprotectant, preferably in a cryoprotective preservation medium containing tenfold-diluted trypticase soy broth and 1% trehalose. As such, successful activity and growth recovery was observed for a diverse set of ammonia-oxidizing cultures.
authors: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium. sven.hoefman@ugent.be
authors: Sven
authors: S
authors: Hoefman
authors: Andreas
authors: A
authors: Pommerening-Röser
authors: Emly
authors: E
authors: Samyn
authors: Paul
authors: P
authors: De Vos
authors: Kim
authors: K
authors: Heylen
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D010084:Oxidation-Reduction; D014199:Trehalose / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D003470:Culture Media; D000641:Ammonia; D014199:Trehalose; D004121:Dimethyl Sulfoxide