title: Thermally modulated cationic copolymer brush on monolithic silica rods for high-speed separation of acidic biomolecules.
abstract: Poly(N-isopropylacrylamide (IPAAm)-co-2-(dimethylamino)ethylmethacrylate(DMAEMA)-co-tert-butylacrylamide (tBAAm)), a thermoresponsive-cationic-copolymer, brush-grafted monolithic-silica column was prepared through surface-initiated atom transfer radical polymerization (ATRP) for effective thermoresponsive anion-exchange chromatography matrices. ATRP-initiator was grafted on monolithic silica-rod surfaces by flowing a toluene solution containing ATRP initiator into monolithic silica-rod columns. IPAAm, DMAEMA, and tBAAm monomers and CuCl/CuCl₂/Me₆TREN, an ATRP catalytic system, were dissolved in 2-propanol, and the reaction solution was pumped into the preprepared initiator modified columns at 25 °C for 16 h. The constructed copolymer-brush structure on monolithic silica-rod surface was confirmed by X-ray photoelectron spectroscopy (XPS), elemental analysis, scanning electron microscopy (SEM) observation, and gel permeation chromatography (GPC) measurement of grafted copolymer. The prepared monolithic silica-rod columns were also characterized by chromatographic analysis. The cationic copolymer brush modified monolithic silica-rod columns were able to separate adenosine nucleotides with a shorter analysis time (4 min) than thermoresponsive copolymer brush-modified silica-bead-packed columns, because of the reduced diffusion path length of monolithic supporting materials. These results indicated that thermoresponsive cationic copolymer brush grafted monolithic silica-rod column prepared by ATRP was a promising tool for analyzing acidic-bioactive compounds with a remarkably short analysis time.
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan.
authors: Kenichi
authors: K
authors: Nagase
authors: Jun
authors: J
authors: Kobayashi
authors: Akihiko
authors: A
authors: Kikuchi
authors: Yoshikatsu
authors: Y
authors: Akiyama
authors: Hideko
authors: H
authors: Kanazawa
authors: Teruo
authors: T
authors: Okano
mesh_terms: D002412:Cations; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D008855:Microscopy, Electron, Scanning; D009711:Nucleotides / Q000302:isolation & purification*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D002412:Cations; D009711:Nucleotides; D011108:Polymers; D012822:Silicon Dioxide