title: Integrating muscle cell biochemistry and whole-body physiology in humans:(31)P-MRS data from the InSight trial.
abstract: We acquired (31)P-MRS data from skeletal muscle of subjects of mixed gender and ethnicity, combined with a panel of physiological characteristics, and tested several long-standing hypotheses regarding relationships between muscle cell biochemistry and whole-body physiology with unusually high statistical power. We hypothesized that i) whole-body VO(2)max would correlate with muscle respiratory capacity, ii) resting muscle phosphocreatine concentration ([PCr]) would negatively correlate with delta efficiency and iii) muscle mitochondrial function would positively correlate with both resting VO(2) and total daily energy expenditure (TDEE). Muscle respiratory capacity explained a quarter of the variation in VO(2)max (r(2) = 26, p < .001, n = 87). There was an inverse correlation between muscle [PCr] and delta efficiency (r = -23, p = 046, n = 87). There was also a correlation between [PCr] recovery halftime and TDEE (r = -23, p = 035, n = 87). Our data not only provide insights into muscle cell chemistry and whole-body physiology but our mixed cohort means that our findings are broadly generalizable.
authors: School of Biological Sciences, University of Essex, Colchester, UK. lindsay.edwards@kcl.ac.uk
authors: Lindsay M
authors: LM
authors: Edwards
authors: Graham J
authors: GJ
authors: Kemp
authors: Renee M
authors: RM
authors: Dwyer
authors: Justin T
authors: JT
authors: Walls
authors: Huddy
authors: H
authors: Fuller
authors: Steven R
authors: SR
authors: Smith
authors: Conrad P
authors: CP
authors: Earnest
mesh_terms: D000328:Adult; D015331:Cohort Studies; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008137:Longitudinal Studies; D008279:Magnetic Resonance Imaging; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000000981:diagnostic imaging / Q000378:metabolism*; D010101:Oxygen Consumption; D010725:Phosphocreatine / Q000378:metabolism; D010759:Phosphorus Isotopes / Q000737:chemistry; D025341:Principal Component Analysis; D011877:Radionuclide Imaging
chemical_list: D010759:Phosphorus Isotopes; D010725:Phosphocreatine
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