title: Antiretroviral bioanalysis methods of tissues and body biofluids.
abstract: Research in the many areas of HIV treatment, eradication and prevention has necessitated measurement of antiretroviral (ARV) concentrations in nontraditional specimen types. To determine the knowledgebase of critical details for accurate bioanalysis, a review of the literature was performed and summarized. Bioanalytical assays for 31 ARVs, including metabolites, were identified in 205 publications measuring various tissues and biofluids. 18 and 30% of tissue or biofluid methods, respectively, analyzed more than one specimen type; 35-37% of the tissue or biofluid methods quantitated more than one ARV. 20 and 76% of tissue or biofluid methods, respectively, were used for the analysis of human specimens. HPLC methods with UV detection predominated, but chronologically MS detection began to surpass. 40% of the assays provided complete intra- and inter-assay validation data, but only 9% of publications provided any stability data with even less for the prevalent ARV in treatments.
authors: HIV Clinical Pharmacology Quality Assurance & HIV Clinical Pharmacology Research Programs, University at Buffalo School of Pharmacy & Pharmaceutical Sciences, Translational Pharmacology Research Core, New York State Center of Excellence in Bioinformatics & Life Sciences, 701 Ellicott Street, Buffalo, NY 14203, USA. rda@buffalo.edu
authors: Robin
authors: R
authors: DiFrancesco
authors: Getrude
authors: G
authors: Maduke
authors: Rutva
authors: R
authors: Patel
authors: Charlene R
authors: CR
authors: Taylor
authors: Gene D
authors: GD
authors: Morse
mesh_terms: D019380:Anti-HIV Agents / Q000032:analysis* / Q000493:pharmacokinetics; D023241:Antiretroviral Therapy, Highly Active / Q000379:methods; D001826:Body Fluids / Q000737:chemistry*; D015658:HIV Infections / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D013048:Specimen Handling / Q000379:methods; D014018:Tissue Distribution
chemical_list: D019380:Anti-HIV Agents
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