title: A fluorescent one-dimensional photonic crystal for  label-free biosensing based on BLOCH surface waves.
abstract: A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
authors: Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, corso Duca degli Abruzzi 24, Turin, Italy. francesca.frascella@polito.it
authors: Francesca
authors: F
authors: Frascella
authors: Serena
authors: S
authors: Ricciardi
authors: Paola
authors: P
authors: Rivolo
authors: Valeria
authors: V
authors: Moi
authors: Fabrizio
authors: F
authors: Giorgis
authors: Emiliano
authors: E
authors: Descrovi
authors: Francesco
authors: F
authors: Michelotti
authors: Peter
authors: P
authors: Munzert
authors: Norbert
authors: N
authors: Danz
authors: Lucia
authors: L
authors: Napione
authors: Maria
authors: M
authors: Alvaro
authors: Federico
authors: F
authors: Bussolino
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003460:Crystallization; D019204:GTP-Binding Proteins / Q000378:metabolism; D017785:Photons; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: D019204:GTP-Binding Proteins
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