title: Analysis of black fungal biofilms occurring at domestic water taps. II: potential routes of entry.
abstract: Formation of tenacious and massive black biofilms was occasionally observed at the water-air interphase of water taps and in associated habitats at several locations in Germany. Exophiala lecanii-corni was proven to be the dominant component of these biofilms. Water utility companies were interested to understand by which route fungi building these black biofilms enter their habitat at affected sites in domestic sanitary. A wide variety of fungi is known to be common in wet indoor environments, as well as in the drinking water resources. Two possible routes of entry are therefore considered as follows: (a) distribution by the drinking water system or (b) a retrograde route of colonisation. Previous compositional analysis revealed that the black constituents of biofilms primarily belong to the herpotrichiellaceous black yeast and relatives. Therefore, a systematic search for black fungi in the drinking water system was performed using Sabouraud's glucose agar medium with chloramphenicol and erythritol-chloramphenicol agar as isolation media. Cadophora malorum was the dominant fungus in the investigated drinking water systems, and samples taken from the house connections (n = 50; 74 %, <200 cfu/L), followed by a so far undescribed Alternaria sp. (28 %; <10 cfu/L) and E. castellanii (26 %; <10 cfu/L). Of note, C. malorum was not present in any previously analysed biofilm. Since E. lecanii-corni was not found in any water sample from the distribution system tested, but represented the most abundant species in dark biofilms previously analysed, a retrograde route of contamination in case of E. lecanii-corni can be assumed.
authors: Institute of Medical Microbiology, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors: Guido
authors: G
authors: Heinrichs
authors: Iris
authors: I
authors: Hübner
authors: Carsten K
authors: CK
authors: Schmidt
authors: G Sybren
authors: GS
authors: de Hoog
authors: Gerhard
authors: G
authors: Haase
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D060766:Drinking Water / Q000382:microbiology*; D004783:Environmental Microbiology; D005658:Fungi / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification*; D005858:Germany; D006801:Humans; D009172:Mycology / Q000379:methods
chemical_list: D003470:Culture Media; D060766:Drinking Water
references: J Vet Diagn Invest. 2009 Jan;21(1):69-79
references: 19139504
references: Mycol Res. 2009 Feb;113(Pt 2):165-72
references: 19010414
references: J Clin Microbiol. 2012 Sep;50(9):3023-30
references: 22785187
references: J Appl Microbiol. 2007 Mar;102(3):774-80
references: 17309627
references: J Hosp Infect. 2005 Jul;60(3):235-9
references: 15949615
references: Appl Environ Microbiol. 2000 Mar;66(3):1249-51
references: 10698803
references: Mycoses. 2012 Mar;55(2):161-7
references: 21736629
references: Mycoses. 2000;43(11-12):409-16
references: 11204358
references: Appl Microbiol Biotechnol. 2007 Mar;74(4):902-10
references: 17103156
references: Rev Iberoam Micol. 2008 Mar;25(1):37-40
references: 18338926
references: Int J Hyg Environ Health. 2006 May;209(3):257-64
references: 16459143
references: Int J Environ Res Public Health. 2010 Aug;7(8):3225-34
references: 20948957
references: Mycopathologia. 2013 Jun;175(5-6):387-97
references: 23385952
references: Water Res. 2004 May;38(9):2258-67
references: 15142786
references: Crit Rev Microbiol. 2009;35(4):340-55
references: 19863383
references: Microbiol Res. 1995 Sep;150(3):311-3
references: 7551736
references: Mycoses. 2008 Mar;51(2):165-9
references: 18254755
references: Lett Appl Microbiol. 1999 Aug;29(2):81-4
references: 10499294
references: Microbiology (Reading). 2011 Sep;157(Pt 9):2611-2618
references: 21636650
references: Curr Microbiol. 2012 Feb;64(2):140-7
references: 22057921
references: Int J Environ Res Public Health. 2011 Mar;8(3):713-32
references: 21556175
references: Antonie Van Leeuwenhoek. 1993;64(1):17-26
references: 8273999
references: Environ Sci Technol. 2006 Apr 1;40(7):2390-5
references: 16646479
references: Med Mycol. 2000;38 Suppl 1:243-50
references: 11204152
references: J Clin Microbiol. 2003 Aug;41(8):3885-9
references: 12904410
references: Appl Environ Microbiol. 2006 Dec;72(12):7586-93
references: 17028226
references: Biotechnol Bioeng. 2001 Dec 5;75(5):550-8
references: 11745130
references: Can J Microbiol. 1982 Jun;28(6):667-71
references: 7116234
references: Med Mycol. 2010 Jun;48(4):622-8
references: 20392154
references: Mycoses. 1998 May-Jun;41(5-6):183-9
references: 9715630
references: J Clin Microbiol. 2011 Dec;49(12):4264-72
references: 21976755
references: Persoonia. 2011 Dec;27:46-72
references: 22403476
references: J Wildl Dis. 1985 Jan;21(1):61-4
references: 3981748
references: J Appl Microbiol. 1998 Dec;85 Suppl 1:214S-224S
references: 21182711
references: Int J Hyg Environ Health. 2002 May;205(4):269-79
references: 12068746
references: Biocontrol Sci. 2010 Jun;15(2):51-6
references: 20616432
references: J Med Vet Mycol. 1995 Sep-Oct;33(5):327-38
references: 8544086
references: Antimicrob Agents Chemother. 2008 Jan;52(1):171-82
references: 17999966
references: Trends Microbiol. 2003 Jan;11(1):30-6
references: 12526852