title: Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells.
abstract: BACKGROUND
  Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression of SATB1 in SCLC.
  
  
  METHODS
  The level of SATB1 was analyzed in SCLC tissues, metastatic lymphoid nodes and adjacent normal lung tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA was constructed and transfected into human SCLC cell line NCI-H446 to evaluate the effects of SATB1-siRNA on cell proliferation, invasion and apoptosis.
  
  
  RESULTS
  SATB1 protein was overexpressed in SCLC tissues and metastasis lymphoid nodes compared with adjacent normal lung tissues. Compared with control group, SATB1-siRNA inhibits the proliferation and invasion of SCLC cells and induces SCLC cells apoptosis statistically (P<0.05) in vitro.
  
  
  CONCLUSIONS
  Our results suggest that SATB1 plays an important role in the metastasis of human SCLC cell.
authors: Department of Thoracic Surgery, the First Affiliated Hospital of Liaoning Medical University, No. 2 People street, 121000, Jinzhou, Liaoning, People's Republic of China.
authors: Bo
authors: B
authors: Huang
authors: Department of Kidney diseases, the First Affiliated Hospital of Liaoning Medical University, 121000, Jinzhou, Liaoning, People's Republic of China.
authors: Hongli
authors: H
authors: Zhou
authors: Department of Thoracic Surgery, the First Affiliated Hospital of Liaoning Medical University, No. 2 People street, 121000, Jinzhou, Liaoning, People's Republic of China.
authors: Xiaodong
authors: X
authors: Wang
authors: Department of Thoracic Surgery, the First Affiliated Hospital of Liaoning Medical University, No. 2 People street, 121000, Jinzhou, Liaoning, People's Republic of China.
authors: Zhiliang
authors: Z
authors: Liu
references: APMIS. 2010 Nov;118(11):855-63
references: 20955458
references: Diagn Pathol. 2012 Aug 30;7:115
references: 22935204
references: Oncologist. 2007 Sep;12(9):1096-104
references: 17914079
references: Cancer Cell Int. 2009 Jul 30;9:18
references: 19642980
references: Breast. 2011 Aug;20(4):309-13
references: 20980149
references: Mol Biol Cell. 1996 Apr;7(4):541-52
references: 8730098
references: Liver Int. 2012 Aug;32(7):1064-78
references: 22583549
references: Mol Immunol. 2008 Nov;46(1):207-11
references: 18722016
references: Cell Cycle. 2005 Aug;4(8):1099-106
references: 15970696
references: J Cancer Res Clin Oncol. 2009 Dec;135(12):1655-63
references: 19513748
references: Blood. 2005 Apr 15;105(8):3330-9
references: 15618465
references: Int J Colorectal Dis. 2012 Feb;27(2):143-50
references: 21870058
references: Oncol Rep. 2010 Oct;24(4):981-7
references: 20811679
references: Nature. 2008 Mar 13;452(7184):187-93
references: 18337816
references: Methods Mol Biol. 2012;820:179-94
references: 22131032
references: J Transl Med. 2012 Jul 28;10:149
references: 22839214
references: Cancer Sci. 2010 Jan;101(1):80-6
references: 19860849
references: J Biol Chem. 2012 Aug 3;287(32):26702-14
references: 22711534
references: Womens Health (Lond). 2008 Jul;4(4):329-32
references: 19072498
references: Cancer Res. 2012 Sep 15;72(18):4724-32
references: 22805310
references: Nat Genet. 2003 May;34(1):42-51
references: 12692553
references: Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):534-7
references: 19304547
references: J Thorac Oncol. 2011 Jul;6(7):1179-89
references: 21597389