title: Vallitalea guaymasensis gen. nov., sp. nov., isolated from marine sediment.
abstract: A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic, halophilic, Gram-stain-negative bacterium, was isolated from sediments of Guaymas Basin. The strain, designated Ra1766G1(T), grew at 20-40 °C (optimum, 30-35 °C) and at pH 6.0-8.0 (optimum, pH 6.5-7.5). It required 0.5-7.5 % NaCl (optimum, 2-3 %) for growth. Sulfate, thiosulfate, elemental sulfur, sulfite, fumarate, nitrate and nitrite were not used as terminal electron acceptors. Strain Ra1766G1(T) used cellobiose, glucose, mannose, maltose, arabinose, raffinose, galactose, ribose, sucrose, pyruvate and xylose as electron donors. The main fermentation product from glucose metabolism was acetate. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, anteiso DMA-C15 : 0 and C16 : 0. The main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, iso-DMA-C15 : 0 glycolipids and phospholipids. The G+C content of the genomic DNA was 31.2 mol%. The closest phylogenetic relatives of strain Ra1766G1(T) were Natranaerovirga pectinivora AP3(T) (92.4 % 16S rRNA gene sequence similarity), Natranaerovirga hydrolytica APP2(T)(90.2 %) and Defluviitalea saccharophila 6LT2(T) (88.9 %). On the basis of phylogenetic inference and phenotypic properties, strain Ra1766G1(T) represents a novel species of a new genus for which the name Vallitalea guaymasensis is proposed. The type strain of the type species is Ra1766G1(T) ( = DSM 24848(T) = JCM17997(T)).
authors: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.|Laboratoire de Microbiologie, IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: Raja
authors: R
authors: Lakhal
authors: Laboratoire de Microbiologie, IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: Nathalie
authors: N
authors: Pradel
authors: Laboratoire de Microbiologie, IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: Anne
authors: A
authors: Postec
authors: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.
authors: Moktar
authors: M
authors: Hamdi
authors: Laboratoire de Microbiologie, IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: Bernard
authors: B
authors: Ollivier
authors: Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, IFREMER, 29280 Plouzané, France.
authors: Anne
authors: A
authors: Godfroy
authors: Laboratoire de Microbiologie, IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: Marie-Laure
authors: ML
authors: Fardeau
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S