title: Prevention of oxidative stress-induced apoptosis of C2C12 myoblasts by a Cichorium intybus root extract.
abstract: Cell injury associated with reactive oxygen species (ROS) has been reported in various muscular disorders. We found that a Cichorium intybus (Cii) extract reduced H(2)O(2)-induced viability loss in C2C12 myoblasts, inhibited oxidative stress-induced apoptosis and increased intracellular heat shock protein 70 (Hsp 70) expression. Cii also inhibited the level of intracellular ceramide. These results indicate that Cii may prevent skeletal muscle atrophy by inducing the expression of Hsp 70 and inhibiting the level of ceramide.
authors: Division of Biological Science and Technology and Yonsei-Fraunhofer Medical Device Lab, College of Science and Technology, Yonsei University, Wonju, Korea.
authors: Yong-Hyeon
authors: YH
authors: Lee
authors: Dae-Hyun
authors: DH
authors: Kim
authors: Yoon Suk
authors: YS
authors: Kim
authors: Tack-Joong
authors: TJ
authors: Kim
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002518:Ceramides / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D018651:Cichorium intybus / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002518:Ceramides; D018840:HSP70 Heat-Shock Proteins; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D000111:Acetylcysteine