title: Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa.
abstract: The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 μg/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 μg/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 μg/l and above. After 2 h exposure at higher test concentrations (10-50 μg/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system.
authors: Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia, Zatisi 728/11, Vodnany 32901, Czech Republic. gazo@frov.jcu.cz
authors: Ievgeniia
authors: I
authors: Gazo
authors: Pavla
authors: P
authors: Linhartova
authors: Anna
authors: A
authors: Shaliutina
authors: Martin
authors: M
authors: Hulak
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004777:Environment; D005399:Fishes / Q000235:genetics* / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000009:adverse effects; D008297:Male; D010080:Oxazoles / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D005659:Fungicides, Industrial; D010080:Oxazoles; D014874:Water Pollutants, Chemical; D000255:Adenosine Triphosphate; D004247:DNA; C025643:vinclozolin