title: Butyric acid retention in gingival tissue induces oxidative stress in jugular blood mitochondria.
abstract: Butyric acid (BA) is a major extracellular metabolite produced by anaerobic periodontopathic bacteria and is commonly deposited in the gingival tissue. BA induces mitochondrial oxidative stress in vitro; however, its effects in vivo were never elucidated. Here, we determined the effects of butyric acid retention in the gingival tissues on oxidative stress induction in the jugular blood mitochondria. We established that BA injected in the rat gingival tissue has prolonged retention in gingival tissues. Blood taken at 0, 60, and 180 min after BA injection was used for further analysis. We isolated blood mitochondria, verified its purity, and measured hydrogen peroxide (H2O2), heme, superoxide (SOD), and catalase (CAT) to determine BA effects. We found that H2O2, heme, SOD, and CAT levels all increased after BA injection. This would insinuate that mitochondrial oxidative stress was induced ascribable to BA.
authors: Department of Microbiology, Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
authors: Marni E
authors: ME
authors: Cueno
authors: Kenichi
authors: K
authors: Imai
authors: Noriko
authors: N
authors: Matsukawa
authors: Takamitsu
authors: T
authors: Tsukahara
authors: Tomoko
authors: T
authors: Kurita-Ochiai
authors: Kuniyasu
authors: K
authors: Ochiai
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000378:metabolism; D020148:Butyric Acid / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005881:Gingiva / Q000378:metabolism*; D006418:Heme / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007601:Jugular Veins; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D020148:Butyric Acid; D006418:Heme; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase
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