title: Effects of adipose stem cell-conditioned medium on the migration of vascular endothelial cells, fibroblasts and keratinocytes.
abstract: Adipose stem cell-conditioned medium (ASC-CM) has been successfully used to treat multiple types of tissue and organ defects, including skin wounds both in vitro and in vivo. However, the mechanisms through which ASC-CM promotes wound healing remain unclear. We hypothesized that the wound healing effect of ASC-CM is mediated in part by the promotion of the migration of vascular endothelial cells, fibroblasts and keratinocytes, the three cell types essential for wound healing. We reported that ASC-CM stimulated the migration of these cells sequentially, and endothelial cells were the first cell type to respond to ASC-CM stimulation (4 h), followed by fibroblasts (12 h) and then keratinocytes (24 h). We also determined the optimal concentration of ASC-CM in stimulating these cells (50% dilution) in addition to the optimal time to intervene in order to maximize the wound healing activity of ASC-CM. Our data suggest an important role for ASC-CM in wound healing, possibly through the synthetic action of multiple adipose stem cell-derived cytokines that in turn promote cell migration. Thus, ASC-CM appears to have significant potential in wound healing applications.
authors: Department of Stomatology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR  China.
authors: Li
authors: L
authors: Hu
authors: Jiajia
authors: J
authors: Zhao
authors: Jiarong
authors: J
authors: Liu
authors: Niya
authors: N
authors: Gong
authors: Lili
authors: L
authors: Chen
keywords: adipose stem cell-conditioned medium; fibroblasts; keratinocytes; migration; vascular endothelial cells
references: J Plast Reconstr Aesthet Surg. 2010 Nov;63(11):1886-92
references: 19969517
references: Biochem Biophys Res Commun. 2008 Apr 11;368(3):455-7
references: 18252194
references: Methods. 2008 Jun;45(2):115-20
references: 18593609
references: Exp Cell Res. 2010 Apr 15;316(7):1271-81
references: 20206158
references: PLoS One. 2010 Aug 17;5(8):e12228
references: 20808927
references: Methods Cell Sci. 2001;23(4):189-96
references: 12486329
references: Biophys J. 1999 May;76(5):2814-23
references: 10233097
references: J Biol Chem. 2010 Nov 12;285(46):35932-43
references: 20826806
references: Biochem Biophys Res Commun. 2002 Jun 28;294(5):940-8
references: 12074567
references: World J Stem Cells. 2011 Apr 26;3(4):25-33
references: 21607134
references: Tissue Eng Part B Rev. 2011 Oct;17(5):373-88
references: 21699433
references: Tissue Eng Part B Rev. 2011 Feb;17(1):25-31
references: 21062224
references: J Dermatol Sci. 2007 Oct;48(1):15-24
references: 17643966
references: J Cell Biochem. 2005 Dec 15;96(6):1110-26
references: 16167340
references: Forensic Sci Int. 2010 Dec 15;203(1-3):93-8
references: 20739128
references: Int J Cancer. 1996 May 29;66(5):664-8
references: 8647630
references: Tissue Eng. 2001 Apr;7(2):211-28
references: 11304456
references: Am J Physiol Cell Physiol. 2002 May;282(5):C1181-90
references: 11940533
references: Wound Repair Regen. 2007 Jul-Aug;15(4):540-8
references: 17650098
references: Regen Med. 2010 Jan;5(1):121-43
references: 20017699
references: Curr Opin Organ Transplant. 2010 Feb;15(1):86-91
references: 19949335