title: APC binds intermediate filaments and is required for their reorganization during cell migration.
abstract: Intermediate filaments (IFs) are components of the cytoskeleton involved in most cellular functions, including cell migration. Primary astrocytes mainly express glial fibrillary acidic protein, vimentin, and nestin, which are essential for migration. In a wound-induced migration assay, IFs reorganized to form a polarized network that was coextensive with microtubules in cell protrusions. We found that the tumor suppressor adenomatous polyposis coli (APC) was required for microtubule interaction with IFs and for microtubule-dependent rearrangements of IFs during astrocyte migration. We also show that loss or truncation of APC correlated with the disorganization of the IF network in glioma and carcinoma cells. In migrating astrocytes, vimentin-associated APC colocalized with microtubules. APC directly bound polymerized vimentin via its armadillo repeats. This binding domain promoted vimentin polymerization in vitro and contributed to the elongation of IFs along microtubules. These results point to APC as a crucial regulator of IF organization and confirm its fundamental role in the coordinated regulation of cytoskeletons.
authors: Cell Polarity, Migration and Cancer Unit, Institut Pasteur, 75724 Paris, Cedex 15, France.
authors: Yasuhisa
authors: Y
authors: Sakamoto
authors: Batiste
authors: B
authors: Boëda
authors: Sandrine
authors: S
authors: Etienne-Manneville
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D051186:Armadillo Domain Proteins / Q000737:chemistry / Q000378:metabolism; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D051186:Armadillo Domain Proteins; D050505:Mutant Proteins
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