title: Neohesperidin dihydrochalcone: presentation of a small molecule activator of mammalian alpha-amylase as an allosteric effector.
abstract: Flavonoids and their precursor trans-chalcone have been reported as inhibitors of mammalian alpha-amylase. With regard to this background, neohesperidin dihydrochalcone (NHDC) effect was investigated toward porcine pancreatic alpha-amylase (PPA), and found to be an activator of the enzyme. The maximal activation (up to threefold) was found to occur at 4.8mM of NHDC, which could be considered to have a high activation profile, with regard to the alpha and beta parameters (alpha<1<beta). NHDC is a non-essential activator of the enzyme and based on the results obtained from modeling tools, it is suggested to interact with PPA at a hydrophilic site located at the N-terminal, far from the active site of the enzyme.
authors: Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Elaheh
authors: E
authors: Kashani-Amin
authors: Bagher
authors: B
authors: Larijani
authors: Azadeh
authors: A
authors: Ebrahim-Habibi
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D020134:Catalytic Domain; D047188:Chalcones / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D062105:Molecular Docking Simulation; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors*; D011485:Protein Binding; D013549:Sweetening Agents / Q000737:chemistry*; D013552:Swine
chemical_list: D047188:Chalcones; D004791:Enzyme Inhibitors; D013549:Sweetening Agents; C013613:neohesperidin dihydrochalcone; D006569:Hesperidin; D055292:Pancreatic alpha-Amylases