title: Effective antimicrobial activity of Cbf-K16 and Cbf-A7 A13 against NDM-1-carrying Escherichia coli by DNA binding after penetrating the cytoplasmic membrane in vitro.
abstract: New Delhi metallo-beta-lactamase-1(NDM-1)-carrying isolates, which are resistant to most clinical used antibiotics except for tigecycline and colistin, have been found worldwide. Cathelicidin-BF (BF-30) is found in the venom of the snake Bungarus fasciatus and exhibits broad antimicrobial activity. Cbf-K(16) and Cbf-A(7) A(13) were obtained by mutating Lys(16), Ala(7), and Ala(13) of BF-30, respectively. To investigate their antimicrobial activities against NDM-1 carrying bacteria, recombinant Escherichia coli BL21 (DE3)-NDM-1 with high NDM-1 activity was constructed by inserting the Klebsiella pneumoniae NDM-1 gene (GenBank accession no. HQ328085) into a pET28a vector and transforming it into E. coli BL21 (DE3). The peptides showed effective antimicrobial activities against NDM-1-carrying E. coli, and the minimum inhibitory concentrations of Cbf-K(16) and Cbf-A(7) A(13) were only 4 and 8 µg/ml, whereas those of minimum bactericidal concentrations were 8 and 16 µg/ml, respectively. A time course experiment showed that colony forming unit counts rapidly decreased, and bacteria were thoroughly eliminated within 3 and 6 h by the Cbf-K(16) and Cbf-A(7) A(13) treatments, respectively. The peptides penetrated the bacterial cell membrane and enabled β-galactosidase leakage, and caused the cytoplasmic membrane to become permeable, and finally bound to the DNA. The genomic DNA of E. coli was completely unable to migrate on an agarose gel after Cbf-K(16) treatment (8 µg/ml). These data demonstrated that Cbf-K(16) and Cbf-A(7) A(13) possess effective antimicrobial activity against drug-resistant strains, including NDM-1 carrying E. coli BL21 (DE3)-NDM-1, by binding to DNA after penetrating the cytoplasmic membrane in vitro, which may have potential therapeutic value for the treatment of NDM-1-carrying bacterial infections.
authors: School of Life Science and Technology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, China.
authors: Qingru
authors: Q
authors: Hao
authors: Hui
authors: H
authors: Wang
authors: Jing
authors: J
authors: Wang
authors: Jie
authors: J
authors: Dou
authors: Min
authors: M
authors: Zhang
authors: Weidong
authors: W
authors: Zhou
authors: Changlin
authors: C
authors: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D054804:Cathelicidins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D011485:Protein Binding; D013211:Staphylococcus aureus; D000078304:Tigecycline; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D054804:Cathelicidins; C582657:Cbf-A7A13 peptide; C582656:Cbf-K16 peptide; D004269:DNA, Bacterial; D047090:beta-Lactams; D000078304:Tigecycline; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; D008911:Minocycline