title: AdipoR1 and AdipoR2 gene expression are regulated by thyroid hormones in adipose tissue.
abstract: The aim of this study was to examine whether the relative gene expression of AdipoR1 and AdipoR2 in rat adipose tissue is altered by thyroid hormones, and whether this might relate to their circulating thyroid hormones and adiponectin levels. Hyper- and hypothyroidism were induced by daily oral administration of levothyroxine and methimazole in rats, respectively, over a 42 days period. Real-time PCR analysis was performed to evaluate the changes in AdipoR1 and AdipoR2 mRNA levels in the adipose tissue on days 15, 28, 42, and also 2 weeks after the cessation of treatment. In response to treatment with methimazole, mRNA levels of AdipoR1 and AdipoR2 decreased in the white adipose tissue compared to the euthyroid rats (p < 0.05). This decline was reversible 2 weeks after treatment cessation. The mRNA levels of AdipoR1 and AdipoR2 were increased in the hyperthyroid group of animals compared to euthyroid control (p < 0.05), and its changes were reversible 2 weeks after treatment cessation (P < 0.05). Adiponectin receptors gene expression levels in the adipose tissue of treated animals have positive correlations with thyroid hormones concentrations. Our results suggest that AdipoR1 and AdipoR2 gene expression is regulated by thyroid hormones in hypo- and hyperthyroidism.
authors: Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors: Samira
authors: S
authors: Seifi
authors: Saeed
authors: S
authors: Nazifi
authors: Mohammad Reza
authors: MR
authors: Tabandeh
authors: Mehdi
authors: M
authors: Saeb
mesh_terms: D052242:Adiponectin / Q000097:blood; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D006980:Hyperthyroidism / Q000097:blood; D007037:Hypothyroidism / Q000097:blood / Q000139:chemically induced; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008713:Methimazole; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D054419:Receptors, Adiponectin / Q000235:genetics* / Q000378:metabolism; D013974:Thyroxine / Q000097:blood* / Q000502:physiology; D014284:Triiodothyronine / Q000097:blood* / Q000502:physiology
chemical_list: D052242:Adiponectin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; C504339:adiponectin receptor 1, rat; C506573:adiponectin receptor 2, rat; D014284:Triiodothyronine; D008713:Methimazole; D013974:Thyroxine
references: J Clin Endocrinol Metab. 1979 Nov;49(5):726-9
references: 226559
references: Horm Metab Res. 2007 May;39(5):314-21
references: 17533572
references: J Endocrinol Invest. 2008 Sep;31(9):745-9
references: 18997483
references: J Physiol Biochem. 2012 Jun;68(2):193-203
references: 22127636
references: Metabolism. 2005 Nov;54(11):1461-6
references: 16253634
references: Clin Endocrinol (Oxf). 2003 Nov;59(5):621-9
references: 14616887
references: Thyroid. 2002 Apr;12(4):287-93
references: 12034052
references: Horm Metab Res. 2011 Jul;43(8):537-44
references: 21626471
references: J Clin Invest. 1977 Mar;59(3):517-27
references: 190269
references: Trends Endocrinol Metab. 2002 Mar;13(2):84-9
references: 11854024
references: Nat Med. 2007 Mar;13(3):332-9
references: 17268472
references: Biochim Biophys Acta. 2005 Nov 15;1726(2):217-23
references: 16039782
references: Horm Metab Res. 2010 Apr;42(4):254-60
references: 20094971
references: J Clin Invest. 2006 Jul;116(7):1784-92
references: 16823476
references: J Physiol Sci. 2012 May;62(3):185-90
references: 22311236
references: J Biol Chem. 2004 Jul 16;279(29):30817-22
references: 15123605
references: Diabetes. 2005 Dec;54(12):3358-70
references: 16306350
references: J Biol Chem. 2003 Jan 24;278(4):2461-8
references: 12431986
references: Thyroid. 2007 May;17(5):421-31
references: 17542672
references: J Biol Chem. 2002 Jul 19;277(29):25863-6
references: 12032136
references: Diabetologia. 2005 Aug;48(8):1585-9
references: 15991021
references: Thyroid. 2006 Apr;16(4):397-402
references: 16646687
references: Horm Metab Res. 2011 Jun;43(7):452-7
references: 21557150
references: Theriogenology. 2010 Mar 15;73(5):659-69
references: 20047754
references: J Endocrinol Invest. 2004 Feb;27(2):RC5-7
references: 15129802
references: Nucleic Acids Res. 2001 May 1;29(9):e45
references: 11328886
references: Metabolism. 2007 Jan;56(1):6-11
references: 17161219
references: J Physiol Biochem. 2003 Mar;59(1):51-60
references: 12903905
references: Nat Med. 2002 Nov;8(11):1288-95
references: 12368907
references: J Physiol Biochem. 2005 Jun;61(2):333-42
references: 16180331
references: Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):2005-10
references: 11172066
references: Am J Physiol Endocrinol Metab. 2001 Jun;280(6):E827-47
references: 11350765
references: Horm Metab Res. 2011 Aug;43(9):653-9
references: 21823062