title: Rapid Chagas diagnosis in clinical settings using a multiparametric assay.
abstract: The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombinant protein and a T. cruzi whole extract) placed in parallel in separate solid phases. This automated diagnostic tool allows the simultaneous analysis of a large number of sera, by determining the presence of antibodies against both antigens by a single run test, with high sensitivity and specificity. The simultaneous analysis of the reactivity against the 2 antigens in a biparametric modality reduces the percentage of false-negative sera and allows a more accurate diagnosis. Using this multiparametric approach, we propose an effective algorithm for the first step of Chagas diagnosis by performing a single test, with time and cost savings.
authors: Byo Research S.r.l., Area Science Park, 16305, Basovizza, Trieste, Italy.
authors: Pasquale
authors: P
authors: Pierimarchi
authors: Laura
authors: L
authors: Cerni
authors: Belkisyolé
authors: B
authors: Alarcón de Noya
authors: Giuseppe
authors: G
authors: Nicotera
authors: Zoraida
authors: Z
authors: Díaz-Bello
authors: Andrea
authors: A
authors: Angheben
authors: Daria
authors: D
authors: Scacciatelli
authors: Manuela
authors: M
authors: Zonfrillo
authors: Giuliano
authors: G
authors: Recinelli
authors: Annalucia
authors: A
authors: Serafino
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D057205:Automation, Laboratory / Q000379:methods; D014355:Chagas Disease / Q000175:diagnosis*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan