title: N-glycosylation of plant-produced recombinant proteins.
abstract: Plants are gaining increasingly acceptance as a production platform for recombinant proteins. One reason for this is their ability to carry out posttranslational protein modifications in a similar if not identical way as mammalian cells. The capability of plants to carry out human-like complex glycosylation is well known. Moreover, the targeted manipulation of the plant N-glycosylation pathway allows the production of proteins carrying largely homogeneous, human-type oligosaccharides. These outstanding results have placed plants in a favourable position compared to other eukaryotic expression systems. This review provides a comprehensive summary of the N-glycosylation of plant-produced recombinant proteins, the possible impact of plant-specific N-glycans on the human immune system, and recent advances in engineering the plant N-glycosylation pathway towards the synthesis of (complex) human-type glycan structures, highlighting challenges and achievements in the application of these powerful technologies.
authors: Wageningen University and Research Centre, Plant Research International, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Dirk
authors: D
authors: Bosch
authors: Alexandra
authors: A
authors: Castilho
authors: Andreas
authors: A
authors: Loos
authors: Arjen
authors: A
authors: Schots
authors: Herta
authors: H
authors: Steinkellner
mesh_terms: D000818:Animals; D006031:Glycosylation; D006801:Humans; D007107:Immune System / Q000378:metabolism; D060847:Metabolic Engineering; D058634:Molecular Farming / Q000379:methods*; D010940:Plant Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D010940:Plant Proteins; D011134:Polysaccharides; D011994:Recombinant Proteins