title: Forward genetic screening for the improved production of fermentable sugars from plant biomass.
abstract: With their unique metabolism and the potential to produce large amounts of biomass, plants are an excellent bio-energy feedstock for a variety of industrial purposes. Here we developed a high-throughput strategy, using the model plant Arabidopsis thaliana, to identify mutants with improved sugar release from plant biomass. Molecular analysis indicates a variety of processes including starch degradation, cell wall composition and polar transport of the plant hormone auxin can contribute to this improved saccharification. To demonstrate translatability, polar auxin transport in maize was either genetically or chemical inhibited and this also resulted in increased sugar release from plant tissues. Our forward genetic approach using Arabidopsis not only uncovers new functions that contribute to cell wall integrity but also demonstrates that information gleaned from this genetic model can be directly translated to monocotyledonous crops such as maize to improve sugar extractability from biomass.
authors: Department of Cell & Systems Biology, University of Toronto, Toronto, Ontario, Canada.
authors: George
authors: G
authors: Stamatiou
authors: Danielle P
authors: DP
authors: Vidaurre
authors: Isaac
authors: I
authors: Shim
authors: Xurong
authors: X
authors: Tang
authors: Wolfgang
authors: W
authors: Moeder
authors: Dario
authors: D
authors: Bonetta
authors: Peter
authors: P
authors: McCourt
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D001692:Biological Transport; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000235:genetics; D002241:Carbohydrates / Q000096:biosynthesis*; D002874:Chromosome Mapping; D016000:Cluster Analysis; D005285:Fermentation; D017343:Genes, Plant; D005820:Genetic Testing; D006868:Hydrolysis; D007210:Indoleacetic Acids / Q000378:metabolism; D009154:Mutation; D030821:Plants, Genetically Modified; D013213:Starch / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D007210:Indoleacetic Acids; D013213:Starch
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