title: High level of antibiotic production in a double polyphosphate kinase and phosphate-binding protein mutant of Streptomyces lividans.
abstract: Phosphate metabolism regulates most of the life processes of microorganisms. In the present work we obtained and studied a Streptomyces lividans ppk/pstS double mutant, which lacks polyphosphate kinase (PPK) and the high-affinity phosphate-binding protein (PstS), impairing at the same time the intracellular storage of polyphosphate and the intake of new inorganic phosphate from a phosphate-limited medium, respectively. In some of the aspects analyzed, the ppk/pstS double mutant was more similar to the wt strain than was the single pstS mutant. The double mutant was thus able to grow in phosphate-limited media, whereas the pstS mutant required the addition of 1 mM phosphate under the assay conditions used. The double mutant was able to incorporate more than one fourth of the inorganic phosphate incorporated by the wt strain, whereas phosphate incorporation was almost completely impaired in the pstS mutant. Noteworthy, under phosphate limitation conditions, the double ppk/pstS mutant showed a higher production of the endogenous antibiotic actinorhodin and the heterologous antitumor 8-demethyl-tetracenomycin (up to 10-fold with respect to the wt strain), opening new possibilities for the use of this strain in the heterologous expression of antibiotic pathways.
authors: Instituto de Biología Funcional y Genómica/Departamento de Microbiología y Genética, Consejo Superior de Investigaciones Científicas CSIC/Universidad de Salamanca, Salamanca, Spain.
authors: Margarita
authors: M
authors: Díaz
authors: Laura
authors: L
authors: Sevillano
authors: Sergio
authors: S
authors: Rico
authors: Felipe
authors: F
authors: Lombo
authors: Alfredo F
authors: AF
authors: Braña
authors: Jose A
authors: JA
authors: Salas
authors: Carmen
authors: C
authors: Mendez
authors: Ramón I
authors: RI
authors: Santamaría
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D017353:Gene Deletion; D060847:Metabolic Engineering; D009279:Naphthacenes / Q000378:metabolism; D028044:Phosphate-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000172:deficiency / Q000378:metabolism*; D048170:Streptomyces lividans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D003470:Culture Media; D009279:Naphthacenes; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D017856:Phosphotransferases (Phosphate Group Acceptor); C080889:5838 DNI; C022424:polyphosphate kinase; C013390:actinorhodin; D000095622:Benzoisochromanequinones