title: Molecular cloning, expression and regulation analysis of the interleukin-6 (IL-6) gene in goose adipocytes.
abstract: 1. Interleukin-6 (IL-6) is a multifunctional cytokine involved in lipid metabolism in adipose tissue. The objective of the study was to identify and characterize the IL-6 gene in the goose.   2. A full-length coding sequence (CDS) of the goose (Anser anser) IL-6 gene was cloned that encoded a 234-amino acid peptide containing a 38-amino acid signal peptide, an IL-6/G-CSF/MGF family consensus pattern and four conserved α-helices. The mature goose IL-6 showed 74% and 39% identities to that of chicken and human, respectively.   3. Quantitative real-time PCR analysis showed that the goose IL-6 was predominantly expressed in liver and was up-regulated in adipocytes by lipopolysaccharide (LPS) and oleic acid.
authors: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang University, Hangzhou, PR China.
authors: F
authors: F
authors: Wang
authors: Y
authors: Y
authors: Tian
authors: G
authors: G
authors: Li
authors: X
authors: X
authors: Chen
authors: H
authors: H
authors: Yuan
authors: D
authors: D
authors: Wang
authors: J
authors: J
authors: Li
authors: J
authors: J
authors: Shen
authors: Z
authors: Z
authors: Tao
authors: Y
authors: Y
authors: Fu
authors: L
authors: L
authors: Lu
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology; D005777:Geese / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D007113:Immunity, Innate; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D019301:Oleic Acid / Q000494:pharmacology; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary
chemical_list: D030161:Avian Proteins; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D019301:Oleic Acid