title: Natural dipeptides as mini-chaperones: molecular mechanism of inhibition of lens βL-crystallin aggregation.
abstract: The effect of histidine-containing dipeptides-carnosine and N-acetylcarnosine-on preventing and treating of cataracts of various etiologic origins has been demonstrated in many studies in vivo, while the precise molecular mechanism of their action is actually obscure. Cataract has been recently attributed to conformational diseases due to the association of lens structure protein aggregation with cataract pathogenesis. In our study, effect of histidine-containing dipeptides-carnosine, N-acetylcarnosine, and anserine-on the UV induced βL-crystallin aggregation was studied in vitro. It was first demonstrated that N-acetylcarnosine and anserine (10-40 mM) considerably suppressed UV induced aggregation of βL-crystallin, while carnosine exerted no effect. Positive correlation between anti-aggregating activity of the compounds used and their hydrophobicity was obtained. It was revealed that N-acetylcarnosine and anserine inhibited the initial stages of the protein photochemical damage. A decrease in the size of protein aggregates was detected in the presence of N-acetylcarnosine and anserine. UV irradiation of βL-crystallin resulted in a significant increase in the number of protein carbonyl groups, and the dipeptides studied did not affect this process. We suppose that N-acetylcarnosine and anserine inhibit βL-crystallin aggregation via formation of a protein-dipeptide complex that prevents macromolecular conformational changes and ensuing protein aggregation.
authors: International Biotechnological Center, MV. Lomonosov Moscow State University, Moscow, Russia. tonica@inbox.ru
authors: Antonina K
authors: AK
authors: Dizhevskaya
authors: Konstantin O
authors: KO
authors: Muranov
authors: Alexander A
authors: AA
authors: Boldyrev
authors: Mikhail A
authors: MA
authors: Ostrovsky
mesh_terms: D000818:Animals; D000861:Anserine / Q000378:metabolism; D002336:Carnosine / Q000031:analogs & derivatives / Q000378:metabolism; D002386:Cataract / Q000378:metabolism; D002417:Cattle; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D007908:Lens, Crystalline / Q000378:metabolism*; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D050050:Protein Carbonylation / Q000528:radiation effects; D011487:Protein Conformation; D014466:Ultraviolet Rays; D038204:beta-Crystallins / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D004151:Dipeptides; D018832:Molecular Chaperones; D038204:beta-Crystallins; C033681:N-acetylcarnosine; D002336:Carnosine; D000861:Anserine