title: Expression of kinA and kinB of Bacillus subtilis, necessary for sporulation initiation, is under positive stringent transcription control.
abstract: Bacillus subtilis cells were exposed to decoyinine to trigger stringent transcription control through inhibition of GMP synthase; amino acid starvation results in the same control through inhibition of GMP kinase by 5'-diphosphate 3'-diphosphate guanosine. The positive and negative transcription control of the stringent genes involves adenine and guanine at the transcription initiation sites, whereby they sense an increase and a decrease in the in vivo ATP and GTP pools, respectively. Decoyinine also induces sporulation in minimum medium. DNA microarray analysis revealed that decoyinine induced two major sensor kinase genes, kinA and kinB, involved in the phosphorelay leading to spore formation. lacZ fusion experiments involving the core promoter regions of kinA and kinB, whose transcription initiation bases are adenines, indicated that decoyinine induced their expression. This induction was independent of CodY and AbrB. When the adenines were replaced with guanines or cytosines, the induction by decoyinine decreased. The in situ replacement of the adenines with guanines actually affected this decoyinine-induced sporulation as well as massive sporulation in nutrient medium. These results imply that operation of the positive stringent transcription control of kinA and kinB, which is mediated by an increase in the ATP pool, is likely a prerequisite for the phosphorelay to transfer the phosphoryl group to Spo0A to initiate sporulation.
authors: Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Japan.
authors: Shigeo
authors: S
authors: Tojo
authors: Kazutake
authors: K
authors: Hirooka
authors: Yasutaro
authors: Y
authors: Fujita
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006160:Guanosine Triphosphate / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010710:Phosphates / Q000378:metabolism; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010710:Phosphates; C075521:kinA protein, Bacillus subtilis; D006160:Guanosine Triphosphate; D010770:Phosphotransferases; D011494:Protein Kinases; C081245:kinB protein, Bacillus subtilis
references: Mol Microbiol. 2002 Oct;46(2):297-304
references: 12406209
references: Curr Opin Microbiol. 2000 Dec;3(6):561-6
references: 11121774
references: J Bacteriol. 1981 Feb;145(2):760-7
references: 6257649
references: Biochim Biophys Acta. 1979 Oct 4;587(2):238-52
references: 114234
references: FEMS Microbiol Lett. 2001 Nov 13;204(2):239-45
references: 11731129
references: Genes Genet Syst. 2009 Aug;84(4):315-8
references: 20057169
references: J Bacteriol. 1981 May;146(2):605-13
references: 6111556
references: Mol Microbiol. 1993 Mar;7(6):967-74
references: 8483422
references: J Biol Chem. 1981 Jul 10;256(13):6866-75
references: 6113248
references: Nucleic Acids Res. 2008 Jan;36(Database issue):D93-6
references: 17962296
references: Mol Microbiol. 1995 Feb;15(4):689-702
references: 7783641
references: Genes Dev. 2001 May 1;15(9):1093-103
references: 11331605
references: Mol Microbiol. 2005 Jun;56(6):1560-73
references: 15916606
references: Mol Cell. 2012 Oct 26;48(2):231-41
references: 22981860
references: Curr Opin Microbiol. 2005 Apr;8(2):203-7
references: 15802253
references: Biochem Biophys Res Commun. 1977 Aug 8;77(3):1118-25
references: 409404
references: Mol Microbiol. 1993 Feb;7(3):337-42
references: 8459762
references: J Bacteriol. 1961 May;81(5):741-6
references: 16561900
references: Mol Gen Genet. 1991 Mar;225(3):347-54
references: 1850083
references: J Bacteriol. 2005 Feb;187(4):1357-68
references: 15687200
references: Curr Opin Microbiol. 2004 Dec;7(6):579-86
references: 15556029
references: EMBO J. 2004 Nov 10;23(22):4473-83
references: 15496987
references: Cell. 1991 Feb 8;64(3):545-52
references: 1846779
references: J Bacteriol. 1982 Aug;151(2):1062-5
references: 6807955
references: Biochimie. 1992 Jul-Aug;74(7-8):619-26
references: 1391039
references: J Bacteriol. 2003 Mar;185(6):1911-22
references: 12618455
references: Mol Microbiol. 1993 Apr;8(1):69-79
references: 8497199
references: J Bacteriol. 1996 Oct;178(20):5910-5
references: 8830686
references: J Bacteriol. 1999 Oct;181(19):6081-91
references: 10498721
references: J Bacteriol. 2010 Aug;192(15):3870-82
references: 20511506
references: J Bacteriol. 1995 Jan;177(1):176-82
references: 8002615
references: Genes Dev. 2005 Sep 15;19(18):2236-44
references: 16166384
references: J Bacteriol. 2008 Sep;190(18):6134-47
references: 18641142
references: Annu Rev Microbiol. 1993;47:441-65
references: 8257105
references: Mol Microbiol. 2008 Jul;69(1):42-54
references: 18433449
references: J Bacteriol. 2010 Dec;192(24):6352-6
references: 20971918
references: J Bacteriol. 2010 Mar;192(6):1573-85
references: 20081037
references: J Bacteriol. 2001 Oct;183(20):5877-84
references: 11566986
references: J Bacteriol. 2011 Nov;193(22):6113-22
references: 21926229
references: J Bacteriol. 1995 Dec;177(23):6999-7002
references: 7592498
references: Microbiology (Reading). 2000 Mar;146 ( Pt 3):573-579
references: 10746760
references: J Biochem. 1998 Jul;124(1):89-97
references: 9644250
references: Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):280-5
references: 19114652
references: J Bacteriol. 1992 May;174(9):2771-8
references: 1569009
references: Mol Microbiol. 2001 Oct;42(1):133-43
references: 11679073