title: Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds.
abstract: Vibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug-resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species.
authors: Centre for Genomics and Applied Gene Technology, Institute of Integrative Omics and Applied Biotechnology (IIOAB), Nonakuri, Purba Medinipur, West Bengal, India. dr.barh@gmail.com
authors: Debmalya
authors: D
authors: Barh
authors: Neha
authors: N
authors: Barve
authors: Krishnakant
authors: K
authors: Gupta
authors: Sudha
authors: S
authors: Chandra
authors: Neha
authors: N
authors: Jain
authors: Sandeep
authors: S
authors: Tiwari
authors: Nidia
authors: N
authors: Leon-Sicairos
authors: Adrian
authors: A
authors: Canizalez-Roman
authors: Anderson Rodrigues
authors: AR
authors: dos Santos
authors: Syed Shah
authors: SS
authors: Hassan
authors: Síntia
authors: S
authors: Almeida
authors: Rommel Thiago Jucá
authors: RT
authors: Ramos
authors: Vinicius Augusto Carvalho
authors: VA
authors: de Abreu
authors: Adriana Ribeiro
authors: AR
authors: Carneiro
authors: Siomar de Castro
authors: Sde C
authors: Soares
authors: Thiago Luiz de Paula
authors: TL
authors: Castro
authors: Anderson
authors: A
authors: Miyoshi
authors: Artur
authors: A
authors: Silva
authors: Anil
authors: A
authors: Kumar
authors: Amarendra Narayan
authors: AN
authors: Misra
authors: Kenneth
authors: K
authors: Blum
authors: Eric R
authors: ER
authors: Braverman
authors: Vasco
authors: V
authors: Azevedo
mesh_terms: D002771:Cholera / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D055808:Drug Discovery; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D006801:Humans; D008024:Ligands; D031721:Piper betle / Q000737:chemistry*; D020543:Proteome; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology; D022223:Vaccines, Subunit / Q000737:chemistry / Q000494:pharmacology; D014734:Vibrio cholerae / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity
chemical_list: D018984:Epitopes, T-Lymphocyte; D008024:Ligands; D020543:Proteome; D054852:Small Molecule Libraries; D022223:Vaccines, Subunit
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