title: Construction of an amperometric D-amino acid biosensor based on D-amino acid oxidase/carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine modified gold electrode.
abstract: An improved D-amino acid biosensor was constructed based on covalent immobilization of D-amino acid oxidase onto carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine hybrid film electrodeposited on gold electrode. The biosensor exhibited an optimal response within 2s at pH 8.0 and 30°C when polarized at 0.09 V. There was a linear relationship between biosensor response (μA) and D-alanine concentration ranging from 0.001 to 0.7 mM. The sensitivity of the biosensor was 54.85 μA cm(-2) mM(-1) with a lower limit of detection of 0.2 μM (signal/noise=3). The enzyme electrode was used 150 times over a period of 4 months. The biosensor measured the d-amino acid level in fruit juices.
authors: Department of Biochemistry, MD (Maharshi Dayanand) University, Rohtak, India.
authors: Suman
authors: S
authors: Lata
authors: Bhawna
authors: B
authors: Batra
authors: Pankaj
authors: P
authors: Kumar
authors: C S
authors: CS
authors: Pundir
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002264:Carboxylic Acids / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D003605:D-Amino-Acid Oxidase / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005638:Fruit / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D053768:Metal Nanoparticles / Q000737:chemistry; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011487:Protein Conformation; D013552:Swine
chemical_list: D000596:Amino Acids; D000814:Aniline Compounds; D002264:Carboxylic Acids; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; C416807:polyaniline; D006046:Gold; D003300:Copper; D003605:D-Amino-Acid Oxidase