title: Strain differences in pleural mesothelial cell reactions induced by potassium octatitanate fibers (TISMO) infused directly into the thoracic cavity.
abstract: Although we have previously reported that the fiber-shaped TISMO, morphologically similar to asbestos, can induce a severe mesothelial reaction in A/J mice, it is important to clarify any strain differences. In the present study, female A/J, C3H/HeN, ICR and C57BL/6 mice were therefore employed as test strains. At the beginning of the experiment, all mice underwent a left thoracotomy and direct administration of 3mg of TISMO particles suspended in 0.2 ml saline into the left thorax. The experiment was terminated after 21 weeks and all groups were sacrificed and the mesothelium and main organs were examined histopathologically. To contribute to mechanistic analysis, iron staining with Berlin blue and Turnbull's blue, and immunostaining for calretinin were also performed. The present experiment demonstrated only minor strain differences in the degree of pleural reaction to TISMO. However, there was clear variation in the iron and lymphocyte accumulation in the pleura and in the liver. This difference in response to TISMO fibers in vivo is important information when considering the development of mesothelioma as an animal model and the extrapolation to human risk from such animal studies.
authors: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan.
authors: Masanao
authors: M
authors: Yokohira
authors: Yuko
authors: Y
authors: Nakano
authors: Keiko
authors: K
authors: Yamakawa
authors: Sosuke
authors: S
authors: Kishi
authors: Fumiko
authors: F
authors: Ninomiya
authors: Kousuke
authors: K
authors: Saoo
authors: Katsumi
authors: K
authors: Imaida
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects* / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry; D007260:Infusion Pumps; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010316:Particle Size; D035422:Pleural Cavity / Q000187:drug effects* / Q000473:pathology; D013045:Species Specificity; D014025:Titanium / Q000737:chemistry / Q000633:toxicity*
chemical_list: C021028:potassium octatitanate; D014025:Titanium
keywords: Lung; Mesothelioma; Mouse; Strain difference; TISMO; TiO(2)