title: Influence of endothelial nitric oxide synthase gene polymorphisms (-786T/C, 4a4b, 894G/T) on Iranian kidney transplant recipients.
abstract: OBJECTIVE
  Nitric oxide is a major mediator in vascular biology and regulator of regional blood flow. Its production is catalyzed by the enzyme endothelial nitric oxide synthase. Protective actions of nitric oxide in ischemia and reperfusion are due to its potential as an antioxidant and anti-inflammatory agent, along with its inhibitory effects on cell signaling pathways of nuclear proteins, such as NF-kB. The endothelial nitric oxide synthase gene polymorphisms affect endothelial nitric oxide synthase activity and are associated with endothelial dysfunction. This study sought to examine the association between single nucleotide polymorphisms in endothelial nitric oxide synthase gene (rs 2070744, 27VNTR, and rs1799983) and the development of acute rejection in renal transplant patients.
  
  
  METHODS
  Sixty-six renal transplant recipients (33 patients with an episode of acute rejection and 33 recipients an episode of acute rejection), between June 2010 and March 2011, were included. The polymorphism was determined by simple polymerase chain reaction and polymerase chain reaction-restriction fragment-length polymorphism analysis.
  
  
  RESULTS
  There was only a significant association of endothelial nitric oxide synthase -786T allele and acute rejection (P = .03). Recessive model of T-786C alleles (TT vs TC+CC) and acute rejection confirmed a significant association (odds ratio: 3.12; 95% CI: 0.01-9.83; P = .025). Haplotype CbG was higher in recipients without rejection as compared to rejection group (OR: 0.42, 95% CI: 0.16-1.13; P < .05). Respecting the endothelial nitric oxide synthase gene 894G/T single nucleotide polymorphisms and 27VNTR, no significant association between the allele/genotype and acute rejection was seen.
  
  
  CONCLUSIONS
  Recipient endothelial nitric oxide synthase gene polymorphisms do not alter the risk of acute rejection after a renal transplant. Rejection is a complex immunologic event. Therefore, finding associated genetic variants demands a multicentric larger sample size.
authors: Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. negarazarpira@yahoo.com
authors: Negar
authors: N
authors: Azarpira
authors: Mahdokht H
authors: MH
authors: Aghdai
authors: Bita
authors: B
authors: Geramizadeh
authors: Ali
authors: A
authors: Bahador
authors: Maryam
authors: M
authors: Ayatolahi
authors: Masumeh
authors: M
authors: Darai
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D005838:Genotype; D006084:Graft Rejection / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015994:Incidence; D007492:Iran; D007676:Kidney Failure, Chronic / Q000208:ethnology / Q000601:surgery*; D016030:Kidney Transplantation / Q000208:ethnology* / Q000502:physiology*; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012189:Retrospective Studies; D012307:Risk Factors; D014180:Transplantation / Q000208:ethnology*
chemical_list: D052250:Nitric Oxide Synthase Type III