title: Modification of agarose with carboxylation and grafting dopamine for promotion of its cell-adhesiveness.
abstract: In order to improve bioactivity of agarose, we modified agarose by carboxylation and grafting dopamine. Under alkaline condition, carboxylated agarose was prepared using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) oxidation system by oxidizing C(6) hydroxyl on D-galactose ring into carboxyl group, and the maximum value of the degree of carboxylation reached 30%. With the increase of the amount of oxidant, the molecular weight of the carboxylated agarose decreased to 4 kDa by gel permeation chromatography (GPC) measure. Carboxylated agarose reacted with dopamine through EDC condensation reaction to obtain agarose grafting dopamine (Ag-g-DA), and the grafting rate of dopamine was determined to be 9.3% by UV spectroscopy at 280 nm. The structures of these modified agaroses were determined by FT-IR and (13)C NMR. Both carboxylated agarose and Ag-g-DA showed no cytotoxicity and promoted cell-adhesiveness.
authors: Biomedical Engineering Institute, Jinan University, Guangzhou 510632, China.
authors: Yixue
authors: Y
authors: Su
authors: Bin
authors: B
authors: Chu
authors: Yuan
authors: Y
authors: Gao
authors: Chaoxi
authors: C
authors: Wu
authors: Lingmin
authors: L
authors: Zhang
authors: Peng
authors: P
authors: Chen
authors: Xiaoying
authors: X
authors: Wang
authors: Shunqing
authors: S
authors: Tang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002234:Carbodiimides / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003497:Cyclic N-Oxides / Q000737:chemistry; D004123:Dimethylamines / Q000737:chemistry; D004298:Dopamine / Q000737:chemistry*; D051379:Mice; D010084:Oxidation-Reduction; D012685:Sepharose / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D002234:Carbodiimides; D002264:Carboxylic Acids; D003497:Cyclic N-Oxides; D004123:Dimethylamines; C569266:N-ethyl-N'-(3-(dimethylamino)-propyl)carbodiimide; D012685:Sepharose; C003959:TEMPO; D004298:Dopamine