title: Interleukin-9 is required for allergic airway inflammation mediated by the cytokine TSLP.
abstract: Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine important for the initiation and development of T helper (Th2) cell-mediated allergic inflammation. In this study, we identified a positive association between interleukin-9 (IL-9) and TSLP concentration in the serum of infants with atopic dermatitis. In primary cell cultures, the addition of TSLP led to an increase in IL-9 production from human and mouse Th9 cells, and induced an increase in signal transducer and activator of transcription 5 (STAT5) activation and binding to the Il9 promoter. In vivo, use of an adoptive transfer model demonstrated that TSLP promoted IL-9-dependent, Th9 cell-induced allergic inflammation by acting directly on T cells. Moreover, transgenic expression of TSLP in the lung stimulated IL-9 production in vivo, and anti-IL-9 treatment attenuated TSLP-induced airway inflammation. Together, our results demonstrate that TSLP promotes Th9 cell differentiation and function and define a requirement for IL-9 in TSLP-induced allergic inflammation.
authors: Department of Pediatrics, HB Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors: Weiguo
authors: W
authors: Yao
authors: Yanlu
authors: Y
authors: Zhang
authors: Rukhsana
authors: R
authors: Jabeen
authors: Evelyn T
authors: ET
authors: Nguyen
authors: David S
authors: DS
authors: Wilkes
authors: Robert S
authors: RS
authors: Tepper
authors: Mark H
authors: MH
authors: Kaplan
authors: Baohua
authors: B
authors: Zhou
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D003876:Dermatitis, Atopic / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007223:Infant; D007249:Inflammation / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016906:Interleukin-9 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D061251:Primary Cell Culture; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012137:Respiratory System / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D050799:STAT5 Transcription Factor / Q000819:agonists / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D057134:Antibodies, Neutralizing; D016207:Cytokines; C508607:IL9 protein, human; D016906:Interleukin-9; D050799:STAT5 Transcription Factor; D000094632:Thymic Stromal Lymphopoietin
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