title: Interleukin-17 expression in murine pressure ulcer tissues.
abstract: To explore the process of pressure ulcer formation, interleukin (IL)-17 expression levels were observed in a mouse model of pressure ulcers. Twenty mice were divided into experimental and control groups (10 mice per group). A mouse model of pressure ulcers was established by inducing ischemia-reperfusion injury on local tissue in the experimental group. Pressure ulcer tissues in the experimental group and normal mouse tissue in the control group were stained using hematoxylin and eosin (H&E) and observed using light microscopy. The protein and mRNA expression levels of IL-17, in mouse pressure ulcer tissues from the experimental group and in the normal tissue from the control group, were determined using real-time PCR and western blot analysis, respectively. The mRNA and protein expression levels of IL-17 were compared between the two groups. H&E staining indicated that striated muscle was arranged orderly and cellular structure was intact in the control group, whilst inflammatory cell infiltration was observed in the muscle tissue of the experimental group. The expression levels of IL-17 mRNA were 0.307±0.058 ng in the experimental group and 0.112±0.042 ng in the control group (P<0.05). The expression levels of the IL-17 protein were 0.434±0.097 ng in the experimental group and 0.181±0.040 ng in the control group (P<0.05). IL-17 expression levels were increased in pressure ulcers, which suggests that IL-17 may be associated with pressure ulcers.
authors: Departments of Special Medical Service, The Central Hospital of Xinxiang, Xinxiang, Henan 453600,  P.R. China.
authors: Wei
authors: W
authors: Cui
authors: Lei-Fang
authors: LF
authors: Yang
authors: Wen-Hong
authors: WH
authors: Wei
authors: Ya-Qin
authors: YQ
authors: Zhu
authors: Xiao
authors: X
authors: Wu
authors: Pei-Xia
authors: PX
authors: Mu
authors: Shu-Ping
authors: SP
authors: Guo
keywords: animal model; interleukin-17; pressure ulcer
references: Br J Dermatol. 2012 Mar;166(3):687-9
references: 21936854
references: J Periodontol. 2007 Jun;78(6):1083-93
references: 17539723
references: Ann Surg. 2003 Aug;238(2):264-74
references: 12894021
references: J Invest Dermatol. 2008 Jul;128(7):1838-51
references: 18219277
references: Wound Repair Regen. 2000 Jan-Feb;8(1):68-76
references: 10760216
references: J Invest Surg. 2004 Jul-Aug;17(4):221-7
references: 15371164
references: Ostomy Wound Manage. 2011 Feb;57(2):55-60
references: 21350273
references: Immunity. 2008 Apr;28(4):454-67
references: 18400188
references: Semin Immunol. 2007 Dec;19(6):362-71
references: 18035554
references: J Rehabil Res Dev. 1995 May;32(2):149-61
references: 7562655
references: Decubitus. 1989 May;2(2):24-8
references: 2665781