title: Virus-induced gene silencing in soybean and common bean.
abstract: Plant viral vectors are useful for transient gene expression as well as for downregulation of gene expression via virus-induced gene silencing (VIGS). When used in reverse genetics approaches, VIGS offers a convenient way of transforming genomic information into knowledge of gene function. Efforts to develop and improve plant viral vectors have expanded their applications and have led to substantial advances needed to facilitate gene function studies in major row crops. Here, we describe a DNA-based Bean pod mottle virus (BPMV) vector system for both gene expression and VIGS in soybean and common bean.
authors: Department of Plant Pathology, Iowa State University, Ames, IA, USA. czhan2@iastate.edu
authors: Chunquan
authors: C
authors: Zhang
authors: Steven A
authors: SA
authors: Whitham
authors: John H
authors: JH
authors: Hill
mesh_terms: D019470:Biolistics; D017798:Comovirus / Q000235:genetics*; D015870:Gene Expression; D055785:Gene Knockdown Techniques / Q000379:methods; D017343:Genes, Plant; D027805:Phaseolus / Q000235:genetics* / Q000821:virology; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified; D034622:RNA Interference; D013025:Glycine max / Q000235:genetics* / Q000821:virology