title: Effect of methanol extracts of Cnidium officinale Makino and Capsella bursa-pastoris on the apoptosis of HSC-2 human oral cancer cells.
abstract: Cnidium officinale Makino and Capsella bursa-pastoris are used as traditional herbs with diverse medicinal effects, including the inhibition of inflammation, reduction of blood pressure and as diuretics, however, the anti-cancer effects of C. officinale Makino and C. bursa-pastoris are poorly defined. The aims of this study were to evaluate the effects of methanol extracts of C. officinale Makino (MECO) and methanol extracts of C. bursa-pastoris (MECB) on the cell growth and apoptosis of HSC-2 human oral cancer cells. MECO and MECB caused growth inhibition and the induction of apoptosis in a concentration-dependent manner in HSC-2 cells. A marked reduction in specificity protein 1 (Sp1) expression following treatment with MECO or MECB was also observed. The downregulation of Sp1 by siRNA resulted in growth inhibition and a reduction of total poly (ADP-ribose) polymerase (PARP) expression. In addition, MECO significantly increased Bax expression levels and MECB increased Bak expression levels and decreased Mcl-1 expression levels. These results suggest that MECO and MECB inhibit cell growth and induce apoptosis via the Sp1 protein, indicating that MECO and MECB are useful bioactive materials and attractive drug candidates for oral cancer.
authors: Department of Oral Medicine, Chonbuk National University; Jeonju 561-756;
authors: Kyung-Eun
authors: KE
authors: Lee
authors: Ji-Ae
authors: JA
authors: Shin
authors: In-Sun
authors: IS
authors: Hong
authors: Nam-Pyo
authors: NP
authors: Cho
authors: Sung-Dae
authors: SD
authors: Cho
keywords: Capsella bursa-pastoris; Cnidium officinale Makino; apoptosis; oral cancer; specificity protein 1
references: Cell Res. 2006 Feb;16(2):203-13
references: 16474435
references: Ai Zheng. 2007 Sep;26(9):996-1000
references: 17927860
references: World J Gastroenterol. 2005 Apr 21;11(15):2213-7
references: 15818728
references: Cancer Res. 2005 Feb 1;65(3):1007-17
references: 15705902
references: J Cell Physiol. 2001 Aug;188(2):143-60
references: 11424081
references: Cancer Res. 2010 Feb 1;70(3):1111-9
references: 20086170
references: Int J Cancer. 2012 Feb 1;130(3):593-601
references: 21469139
references: J Clin Oncol. 2009 Jun 1;27(16):2712-25
references: 19414669
references: Pharmacol Res. 2009 Jun;59(6):365-78
references: 19429468
references: Eur J Cancer. 2005 Nov;41(16):2438-48
references: 16209919
references: Anticancer Res. 2000 Mar-Apr;20(2A):661-7
references: 10810337
references: Cell Death Differ. 1999 Sep;6(9):873-82
references: 10510469
references: J Biol Chem. 2012 Oct 12;287(42):35234-35243
references: 22896709
references: Zhonghua Zhong Liu Za Zhi. 2007 Feb;29(2):107-11
references: 17645844
references: Oral Dis. 2011 Mar;17(2):162-70
references: 20659264
references: Head Neck. 2013 Jul;35(7):992-8
references: 22733715
references: Cancer Res. 2008 Jul 1;68(13):5345-54
references: 18593936
references: Cancer Res. 2007 Mar 15;67(6):2816-23
references: 17363604
references: Clin Cancer Res. 2003 Dec 15;9(17):6371-80
references: 14695137
references: Med Chem. 2011 Sep;7(5):518-25
references: 22022994
references: Planta Med. 2010 Aug;76(11):1075-9
references: 20486070
references: Eur J Cancer Prev. 2011 Sep;20(5):417-25
references: 21597379