title: Structural studies of the pectic polysaccharide from Siberian fir (Abies sibirica Ledeb.).
abstract: The pectic polysaccharide named abienan AS-A was isolated from the wood greenery of Abies sibirica using dilute hydrochloric acid (pH 4.0) at 70°C. The structure of abienan AS-A was elucidated using sugar composition analysis, ion-exchange chromatography and partial acid hydrolysis followed by NMR spectroscopy. The linear region of abienan AS-A was shown to contain linear 1,4-α-D-galactopyranosyluronan partially substituted with methyl esters or 3-O-acetyl groups and rhamnogalacturonan blocks consisting of 1,4-α-D-galacturonan partially substituted with methyl ester groups and connected by 2-O-substituted α-rhamnopyranose residues. The branched region of abienan AS-A was found to be made of RG-I. The side chains of RG-I were shown to contain 1,4-β-galactan and branched arabinan. Some 4-O-substituted β-galactopyranose residues were shown to be attached to the 4-position of the 2-O-substituted α-rhamnopyranose residues of the RG-I backbone. The arabinan groups were made up of a 1,5-linked α-L-arabinofuranan backbone that was 3-O-, 2-O-, and 2,3-di-O-substituted with the terminal and 1,3-linked α-L-arabinofuranose residues.
authors: Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 49, Pervomaiskaya Str., 167982 Syktyvkar, Russia. makarowa.elena-ma@yandex.ru
authors: Elena N
authors: EN
authors: Makarova
authors: Olga A
authors: OA
authors: Patova
authors: Evgeny G
authors: EG
authors: Shakhmatov
authors: Sergey P
authors: SP
authors: Kuznetsov
authors: Yury S
authors: YS
authors: Ovodov
mesh_terms: D028202:Abies / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010368:Pectins; D006851:Hydrochloric Acid