title: Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines.
abstract: Thyroid carcinomas account for a minority of all malignant tumours but, after those of the gonads, they represent the most common forms of endocrine cancers. They include several types, among which the papillary thyroid cancer (PTC) and the anaplastic thyroid cancer (ATC) are the best known. The two hystotypes display significant biological and clinical differences: PTC is a well differentiated form of tumour with a high incidence and a good prognosis, while the ATC is less frequent but represents one of the most aggressive endocrine tumours with morphological features of an undifferentiated type. To date, as far as we know, no conclusive studies, useful to design arrays of molecular markers, have been published illustrating the phenotypic and proteomic differences between these two tumours. The aim of this work was to perform a comparative analysis of two thyroid cancer cell lines, derived respectively from papillary (BCPAP) and anaplastic (8505C) thyroid carcinomas. The comparative analysis included cell behaviour assays and proteomic analysis by 2D-PAGE and mass spectrometry. The results have highlighted a new proteomic signature for the anaplastic carcinoma-derived cells, consistent with their high proliferation rate, motility propensity and metabolic shift, in relation to the well-differentiated PTC cells.
authors: Center of Experimental Oncobiology (C.OB.S.), La Maddalena Hospital III Level Oncological Dept., Palermo, Italy.
authors: Rosa
authors: R
authors: Musso
authors: Gianluca
authors: G
authors: Di Cara
authors: Nadia Ninfa
authors: NN
authors: Albanese
authors: Maria Rita
authors: MR
authors: Marabeti
authors: Patrizia
authors: P
authors: Cancemi
authors: Dèsirèe
authors: D
authors: Martini
authors: Ester
authors: E
authors: Orsini
authors: Carla
authors: C
authors: Giordano
authors: Ida
authors: I
authors: Pucci-Minafra
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D000077273:Thyroid Cancer, Papillary; D065646:Thyroid Carcinoma, Anaplastic; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020543:Proteome
keywords: 8505C cells; Anaplastic; BCPAP cells; Papillary; Proteomics; Thyroid cancer