title: Separation of amino acids by capillary electrophoresis with light-emitting diode-induced fluorescence in the presence of electroosmotic flow.
abstract: In this chapter, we describe a method to identify amino acids (AA) by capillary electrophoresis in conjunction with light-emitting diode-induced fluorescence (LEDIF). First, amino acids labeled with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide are converted to highly fluorescent cyanobenz[f]isoindole (CBI) derivatives. Next, they are separated by gel electrophoresis in the presence of EOF. In the process, the CBI products were excited by a violet LED to produce green fluorescence. In addition to the optical setup of light-emitting diode-induced fluorescence, the preparation of poly(ethylene) oxide for amino acid separation is also described in this chapter.
authors: Department of Chemistry, National Kaohsiung Normal University, Kaohsiung, Taiwan.
authors: Ming-Mu
authors: MM
authors: Hsieh
authors: Po-Ling
authors: PL
authors: Chang
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D053841:Electroosmosis / Q000295:instrumentation / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry; D008027:Light; D009281:Naphthalenes / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D007211:Indoles; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde