title: Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation.
abstract: Pulsed electron-electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an α-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions.
authors: Research Group EPR Spectroscopy, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors: Igor
authors: I
authors: Tkach
authors: Soraya
authors: S
authors: Pornsuwan
authors: Claudia
authors: C
authors: Höbartner
authors: Falk
authors: F
authors: Wachowius
authors: Snorri Th
authors: ST
authors: Sigurdsson
authors: Tatiana Y
authors: TY
authors: Baranova
authors: Ulf
authors: U
authors: Diederichsen
authors: Giuseppe
authors: G
authors: Sicoli
authors: Marina
authors: M
authors: Bennati
mesh_terms: D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D013113:Spin Labels
chemical_list: D009589:Nitrogen Oxides; D009841:Oligonucleotides; D010455:Peptides; D013113:Spin Labels; C510055:r(GGCGBrUGCGCU)2; C039900:nitroxyl