title: Redundancy among manganese peroxidases in Pleurotus ostreatus.
abstract: Manganese peroxidases (MnPs) are key players in the ligninolytic system of white rot fungi. In Pleurotus ostreatus (the oyster mushroom) these enzymes are encoded by a gene family comprising nine members, mnp1 to -9 (mnp genes). Mn(2+) amendment to P. ostreatus cultures results in enhanced degradation of recalcitrant compounds (such as the azo dye orange II) and lignin. In Mn(2+)-amended glucose-peptone medium, mnp3, mnp4, and mnp9 were the most highly expressed mnp genes. After 7 days of incubation, the time point at which the greatest capacity for orange II decolorization was observed, mnp3 expression and the presence of MnP3 in the extracellular culture fluids were predominant. To determine the significance of MnP3 for ligninolytic functionality in Mn(2+)-sufficient cultures, mnp3 was inactivated via the Δku80 strain-based P. ostreatus gene-targeting system. In Mn(2+)-sufficient medium, inactivation of mnp3 did not significantly affect expression of nontargeted MnPs or their genes, nor did it considerably diminish the fungal Mn(2+)-mediated orange II decolorization capacity, despite the significant reduction in total MnP activity. Similarly, inactivation of either mnp4 or mnp9 did not affect orange II decolorization ability. These results indicate functional redundancy within the P. ostreatus MnP gene family, enabling compensation upon deficiency of one of its members.
authors: Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
authors: Tomer M
authors: TM
authors: Salame
authors: Doriv
authors: D
authors: Knop
authors: Dana
authors: D
authors: Levinson
authors: Oded
authors: O
authors: Yarden
authors: Yitzhak
authors: Y
authors: Hadar
mesh_terms: D001391:Azo Compounds / Q000378:metabolism; D001557:Benzenesulfonates / Q000378:metabolism; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D008345:Manganese / Q000378:metabolism; D005810:Multigene Family; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics*
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D003470:Culture Media; D008345:Manganese; D010544:Peroxidases; C051129:manganese peroxidase; C014722:2-naphthol orange
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