title: A Novel Peroxidase CanPOD Gene of Pepper Is Involved in Defense Responses to Phytophtora capsici Infection as well as Abiotic Stress Tolerance.
abstract: Peroxidases are involved in many plant processes including plant defense responses to biotic and abiotic stresses. We isolated a novel peroxidase gene CanPOD from leaves of pepper cultivar A3. The full-length gene has a 1353-bp cDNA sequence and contains an open reading frame (ORF) of 975-bp, which encodes a putative polypeptide  of 324 amino acids with a theoretical protein size of 34.93 kDa. CanPOD showed diverse expression levels in different tissues of pepper plants. To evaluate the role of CanPOD in plant stress responses, the expression patterns of CanPOD were examined using  Real-Time RT-PCR. The results indicated that CanPOD was significantly induced by Phytophtora capsici. Moreover, CanPOD was also up-regulated in leaves after salt and drought stress treatments. In addition, CanPOD expression was strongly induced by signaling hormones salicylic acid (SA). In contrast, CanPOD was not highly expressed after treatment with cold. Meanwhile, in order to further assess the role of gene CanPOD in defense response to P. capsici attack, we performed a loss-of-function experiment using the virus-induced gene silencing (VIGS) technique in pepper plants. In comparison to  the control plant, the expression levels of CanPOD were obviously decreased in  CanPOD-silenced pepper plants. Furthermore, we analyzed the effect of P. capsici on detached-leaves and found that the CanPOD-silenced plant leaves were highly susceptible to P. capsici infection. Taken together, our results suggested that CanPOD is involved in defense responses to P. capsici infection as well as abiotic stresses in pepper plants.
authors: College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China. gzhh168@yahoo.com.cn.
authors: Jun-E
authors: JE
authors: Wang
authors: Ke-Ke
authors: KK
authors: Liu
authors: Da-Wei
authors: DW
authors: Li
authors: Ying-Li
authors: YL
authors: Zhang
authors: Qian
authors: Q
authors: Zhao
authors: Yu-Mei
authors: YM
authors: He
authors: Zhen-Hui
authors: ZH
authors: Gong
references: Mol Cells. 2004 Apr 30;17(2):377-80
references: 15179058
references: J Biol Chem. 2008 Dec 5;283(49):34197-203
references: 18852264
references: Planta. 2008 Mar;227(4):867-81
references: 18224366
references: Mol Plant Pathol. 2000 Jul 1;1(4):223-32
references: 20572969
references: Plant Physiol. 2010 Feb;152(2):948-67
references: 19939946
references: Gene. 2002 Apr 17;288(1-2):129-38
references: 12034502
references: Mol Plant Microbe Interact. 2011 Mar;24(3):336-51
references: 21091159
references: Trends Plant Sci. 2004 Nov;9(11):534-40
references: 15501178
references: Tree Physiol. 2006 Nov;26(11):1405-12
references: 16877325
references: Plant Mol Biol. 2008 Jul;67(5):519-33
references: 18427932
references: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:557-583
references: 15012246
references: Mol Plant Pathol. 2012 May;13(4):329-37
references: 22013895
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Nature. 2006 Nov 16;444(7117):323-9
references: 17108957
references: Plant Sci. 2000 Jun 12;155(1):85-100
references: 10773343
references: Mol Plant Microbe Interact. 2003 Mar;16(3):196-205
references: 12650451
references: Plant Cell. 2011 Feb;23(2):823-42
references: 21335377
references: Biochem Biophys Res Commun. 2011 Dec 9;416(1-2):24-30
references: 22086175
references: Phytopathology. 2010 Aug;100(8):774-83
references: 20626281
references: Annu Rev Phytopathol. 2005;43:205-27
references: 16078883
references: Mol Genet Genomics. 2003 Jul;269(4):542-52
references: 12802681
references: Plant Cell Physiol. 2001 May;42(5):462-8
references: 11382811
references: Plant Physiol. 2007 Nov;145(3):890-904
references: 17905862
references: Plant Cell Environ. 2013 Apr;36(4):757-74
references: 22994555
references: Plant Cell Rep. 2005 Jul;24(5):255-65
references: 15856234
references: Eur J Biochem. 2002 Dec;269(24):6063-81
references: 12473102
references: Phytochemistry. 2004 May;65(10):1331-42
references: 15231406
references: Plant Physiol. 1997 Jan;113(1):249-257
references: 12223604
references: New Phytol. 2008;180(2):501-510
references: 18681935
references: Mol Plant Microbe Interact. 2005 Jul;18(7):730-41
references: 16042019
references: FEBS Lett. 2000 Apr 14;471(2-3):245-50
references: 10767432
references: Planta. 2009 Jan;229(2):383-91
references: 18956212
references: Planta. 2008 Jun;228(1):61-78
references: 18327607
references: Theor Appl Genet. 2008 Dec;118(1):15-27
references: 18795251
references: Plant J. 2002 May;30(4):415-29
references: 12028572
references: Plant J. 2002 Sep;31(6):777-86
references: 12220268
references: Phytopathology. 2011 Jun;101(6):732-40
references: 21281115
references: Plant Physiol. 1990 Oct;94(2):485-91
references: 16667738
references: Plant Physiol. 2006 Jan;140(1):249-62
references: 16377744
references: Ann Bot. 2003 Jan;91 Spec No:179-94
references: 12509339
references: Planta. 2012 Jun;235(6):1369-82
references: 22210598
references: Phytochemistry. 2000 May;54(1):19-22
references: 10846741
references: Plant Physiol. 1998 Feb 1;116(2):659-69
references: 9490766
references: Plant Physiol Biochem. 2004 Dec;42(10):763-72
references: 15596095
references: Appl Microbiol Biotechnol. 2003 Feb;60(6):665-70
references: 12664144