title: [Effects of canine IL-2 and IL-7 genes on enhancing immunogenicity of canine parvovirus VP2 gene vaccine in mice].
abstract: OBJECTIVE
  To investigate the effects of canine interleukin-2 (cIL-2) and cIL-7 genes on enhancing the immunogenicity of canine parvovirus (CPV) VP2 DNA vaccine.
  
  
  METHODS
  The bicistronic vectors of cIL-2 and cIL-7 genes were constructed using the eukaryotic expression vector containing internal ribosome entry site (IRES). The cIL-2/ cIL-7 dicistronic vector plus previously constructed vectors, including CPV VP2 DNA vaccine vector, cIL-2 vector and cIL-7 vector, were used to co-immunize mice with different combinations, consisting of VP2 alone, VP2 + cIL-2, VP2 + cIL-7 and VP2 + cIL-2/cIL-7. The VP2-specific antibody levels in immunized mice were measured by ELISA at different time post-immunization. The proliferation indices and interferon-gamma expression were measured by lymphocyte proliferation assay and ELISA, respectively.
  
  
  RESULTS
  The cIL-2/cIL-7 bicistronic vector was correct and could mediate cIL-2 and cIL-7 gene expression in eukaryotic cells. Immunization results revealed that the antibody titers and the neutralizing antibody levels of the mice co-immunized with VP2 + cIL-7/cIL-2 vectors were significantly higher than that with either VP2 + cIL-2 vectors or VP2 + cIL-7 vectors (P < 0.05). The lymphocyte proliferation indices of VP2 + cIL-7/cIL-2 vector-immunized mice were also higher than that of other two groups although not statistically significant. However, the IFN-gamma expression levels of VP2 + cIL-7/cIL-2 vector-immunized mice were significantly higher than other immunized mice (P < 0.05).
  
  
  CONCLUSIONS
  The cIL-2 and cIL-7 genes showed the significant synergic effects on enhancing the immunogenecity of CPV VP2 DNA vaccine.
authors: Department of Basic Veterinary Medicine, College of Animal Science and Technology, Agricultural University of Hebei, North China Research Center of Animal Epidemic Pathogen Biology, Ministry of Agriculture of China, Baoding 071001, China. flying7700@163.com
authors: Huihui
authors: H
authors: Chen
authors: Fei
authors: F
authors: Zhong
authors: Xiujin
authors: X
authors: Li
authors: Lu
authors: L
authors: Wang
authors: Yan
authors: Y
authors: Sun
authors: Changai
authors: C
authors: Neng
authors: Kao
authors: K
authors: Zhang
authors: Wenyan
authors: W
authors: Li
authors: Jiexia
authors: J
authors: Wen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D004285:Dogs; D005260:Female; D007114:Immunization; D007376:Interleukin-2 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015851:Interleukin-7 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010322:Parvoviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D007376:Interleukin-2; D015851:Interleukin-7; D011993:Recombinant Fusion Proteins; D014765:Viral Vaccines