title: Extended storage of autologous apheresis platelets in plasma.
abstract: OBJECTIVE
  The purpose of our studies was to determine the effects of extended platelet storage on poststorage platelet viability.
  
  
  METHODS
  Normal subjects were recruited to donate platelets using two different apheresis systems: either the COBE Spectra (n = 58) or the Haemonetics MCS+ (n = 84). Platelet recovery and survival data from the two systems were compared with each other and with in vitro measurements of the stored platelets.
  
  
  RESULTS
  There were no significant differences in either platelet recoveries or survivals between the two machines between 1 and 8 days of storage. Combining the data from both machines, platelet recoveries decreased by 2.6% and survivals by 0.3 days/storage day. In vitro assays did not predict either platelet recoveries or survivals during storage for 5-8 days. After 9 days of storage, pHs were unacceptable (≤ 6.1), suggesting that 8 days will be the longest possible storage time.
  
  
  CONCLUSIONS
  These data suggest that, if stored platelet bacterial contamination issues are resolved, significant extension of platelet storage times is possible.
authors: Puget Sound Blood Center, Seattle, WA 98104-1256, USA. sjslichter@psbc.org
authors: S J
authors: SJ
authors: Slichter
authors: D
authors: D
authors: Bolgiano
authors: J
authors: J
authors: Corson
authors: M K
authors: MK
authors: Jones
authors: T
authors: T
authors: Christoffel
authors: E
authors: E
authors: Pellham
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D010983:Plateletpheresis / Q000379:methods*; D012189:Retrospective Studies; D013997:Time Factors
references: Proc Soc Exp Biol Med. 1998 Feb;217(2):219-27
references: 9452147
references: Transfusion. 2003 Jan;43(1):2-6
references: 12519424
references: Transfusion. 1997 Apr;37(4):423-35
references: 9111281
references: Vox Sang. 2008 May;94(4):292-8
references: 18331381
references: Transfusion. 1975 Sep-Oct;15(5):414-21
references: 823
references: Transfusion. 1998 Jan;38(1):31-40
references: 9482391
references: Comput Biol Med. 1988;18(4):305-15
references: 3409677
references: Transfusion. 2005 Jul;45(7):1143-50
references: 15987360
references: Transfusion. 2004 Jun;44(6):891-9
references: 15157257
references: Transfusion. 2009 May;49(5):903-12
references: 19170988
references: Transfusion. 2003 Sep;43(9):1230-7
references: 12919425
references: Anesthesiology. 1995 Dec;83(6):1167-72
references: 8533908
references: Am J Hematol. 1998 Jul;58(3):165-76
references: 9662266
references: Transfusion. 2004 Jun;44(6):877-85
references: 15157255
references: J Trauma. 2006 Jun;60(6 Suppl):S78-82
references: 16763485
references: Vox Sang. 2009 Nov;97(4):355-7
references: 19682347
references: Transfus Med. 2003 Aug;13(4):173-87
references: 12880388
references: Transfusion. 2006 Oct;46(10):1763-9
references: 17002633
references: Transfusion. 2002 Jul;42(7):847-54
references: 12375656
references: Am J Clin Pathol. 1999 May;111(5):693-9
references: 10230361
references: Transfusion. 1986 Jan-Feb;26(1):37-42
references: 3080829
references: Vox Sang. 1998;74 Suppl 2:207-16
references: 9704447