title: Application of GC-MS/MS for the analysis of tobacco alkaloids in cigarette filler and various tobacco species.
abstract: This publication reports the first known use of gas chromatography-tandem mass spectrometry for the quantitation of five minor tobacco alkaloids (nornicotine, myosmine, anabasine, anatabine, and isonicoteine) in various tobacco samples. A summary of the concentrations of these minor alkaloid levels in the filler from 50 popular cigarette brands were found to be 659-986 μg/g nornicotine, 8.64-17.3 μg/g myosmine, 127-185 μg/g anabasine, 927-1390 μg/g anatabine, and 23.4-45.5 μg/g isonicoteine. Levels of minor alkaloids found in reference cigarettes (1R5F, 2R4F, 3R4F, CM4, and CM6) as well as burley, flue-cured, oriental, reconstituted, and Nicotiana rustica and Nicotiana glauca tobacco types are also reported. Quantitation of the minor tobacco alkaloids is important because the alkaloids have been shown to be precursors of carcinogenic tobacco specific N'-nitrosamines.
authors: Tobacco and Volatiles Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, United States. jlisko@cdc.gov
authors: Joseph G
authors: JG
authors: Lisko
authors: Stephen B
authors: SB
authors: Stanfill
authors: Bryce W
authors: BW
authors: Duncan
authors: Clifford H
authors: CH
authors: Watson
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D062789:Tobacco Products / Q000032:analysis*
chemical_list: D000470:Alkaloids
references: J Chromatogr A. 1996 Oct 4;746(1):131-6
references: 8885385
references: Cancer Res. 1985 Mar;45(3):935-44
references: 3882226
references: J Chromatogr A. 2007 Sep 14;1164(1-2):313-9
references: 17644105
references: Anal Chem. 2002 Oct 1;74(19):4878-84
references: 12380807
references: Arch Int Pharmacodyn Ther. 1965 Aug;156(2):363-79
references: 5868944
references: Int J Cancer. 1991 Feb 1;47(3):348-51
references: 1993541
references: J Med Toxicol. 2011 Mar;7(1):47-51
references: 20652661
references: Forensic Sci Int. 2002 Jul 17;127(3):208-17
references: 12175951