title: HPLC-based quantification of in vitro N-terminal acetylation.
abstract: Protein N-terminal acetylation is a widespread modification in eukaryotes catalyzed by N-terminal acetyltransferases (NATs). The various NATs and their specific substrate specificities and catalytic mechanisms are far from fully understood. We here describe an in vitro method based on reverse-phase HPLC to quantitatively measure in vitro acetylation of NAT oligopeptide substrates, enabling the determination of NAT specificity as well as kinetic parameters.
authors: Department of Molecular Biology, University of Bergen, Bergen, Norway.
authors: Rune H
authors: RH
authors: Evjenth
authors: Petra
authors: P
authors: Van Damme
authors: Kris
authors: K
authors: Gevaert
authors: Thomas
authors: T
authors: Arnesen
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D063213:N-Terminal Acetyltransferase E / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity
chemical_list: D009842:Oligopeptides; D063213:N-Terminal Acetyltransferase E