title: Isolation of the putative biosynthetic gene cluster of 1-deoxynojirimycin by Bacillus amyloliquefaciens 140N, its production and application to the fermentation of soybean paste.
abstract: The 1-deoxynojirimycin (DNJ) biosynthetic gene cluster of Bacillus amyloliquefaciens 140N isolated from traditional Korean fermented food was isolated by PCR screening. It showed 78.9% inhibitory activity against α-glucosidase and produced 0.8 g/L of DNJ in an optimized medium containing 2% soluble starch, 1% tryptone, 0.05% KH(2)PO(4), and 0.05% (NH(2))(4)SO(4). Soybean paste fermented with B. amyloliquefaciens 140N produced DNJ with 84.4% inhibitory activity.
authors: Division of Bioengineering, University of Incheon, Incheon, Republic of Korea.
authors: Myung-Ji
authors: MJ
authors: Seo
authors: Young-Do
authors: YD
authors: Nam
authors: So-Young
authors: SY
authors: Lee
authors: So-Lim
authors: SL
authors: Park
authors: Sung-Hun
authors: SH
authors: Yi
authors: Seong-Il
authors: SI
authors: Lim
mesh_terms: D017485:1-Deoxynojirimycin / Q000378:metabolism*; D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005516:Food Microbiology; D065089:Glycoside Hydrolase Inhibitors; D005810:Multigene Family; D013025:Glycine max / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D065089:Glycoside Hydrolase Inhibitors; D017485:1-Deoxynojirimycin; D000520:alpha-Glucosidases