title: Cryo-electron microscopy three-dimensional structure of the jumbo phage ΦRSL1 infecting the phytopathogen Ralstonia solanacearum.
abstract: ϕRSL1 jumbo phage belongs to a new class of viruses within the Myoviridae family. Here, we report its three-dimensional structure determined by electron cryo microscopy. The icosahedral capsid, the tail helical portion, and the complete tail appendage were reconstructed separately to resolutions of 9 Å, 9 Å, and 28 Å, respectively. The head is rather complex and formed by at least five different proteins, whereas the major capsid proteins resemble those from HK97, despite low sequence conservation. The helical tail structure demonstrates its close relationship to T4 sheath proteins and provides evidence for an evolutionary link of the inner tail tube to the bacterial type VI secretion apparatus. Long fibers extend from the collar region, and their length is consistent with reaching the host cell surface upon tail contraction. Our structural analyses indicate that ϕRSL1 is an unusual member of the Myoviridae that employs conserved protein machines related to different phages and bacteria.
authors: Unit of Virus Host-Cell Interactions, UMI 3265 UJF-EMBL-CNRS, 6 Rue Jules Horowitz, 38042 Grenoble Cedex, France.
authors: Grégory
authors: G
authors: Effantin
authors: Ryosuke
authors: R
authors: Hamasaki
authors: Takeru
authors: T
authors: Kawasaki
authors: Maria
authors: M
authors: Bacia
authors: Christine
authors: C
authors: Moriscot
authors: Winfried
authors: W
authors: Weissenhorn
authors: Takashi
authors: T
authors: Yamada
authors: Guy
authors: G
authors: Schoehn
mesh_terms: D001435:Bacteriophages / Q000648:ultrastructure*; D002213:Capsid / Q000648:ultrastructure; D036022:Capsid Proteins / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D043368:Ralstonia solanacearum / Q000821:virology*; D017376:Viral Tail Proteins / Q000648:ultrastructure
chemical_list: D036022:Capsid Proteins; D017376:Viral Tail Proteins