title: Regulation of secondary wall synthesis and cell death by NAC transcription factors in the monocot Brachypodium distachyon.
abstract: In several dicotyledonous species, NAC transcription factors act as master switches capable of turning on programmes of secondary cell-wall synthesis and cell death. This work used an oestradiol-inducible system to overexpress the NAC transcription factor BdSWN5 in the monocot model Brachypodium distachyon. This resulted in ectopic secondary cell-wall formation in both roots and shoots. Some of the genes upregulated in the process were a secondary cell-wall cellulose synthase (BdCESA4), a xylem-specific protease (BdXCP1) and an orthologue of AtMYB46 (BdMYB1). While activation of BdMYB1 may not be direct, this study showed that BdSWN5 is capable of transactivating the BdXCP1 promoter through two conserved binding sites. In the course of Brachypodium development, the BdXCP1 promoter was observed to be active in all types of differentiating tracheary elements. Together, these results suggest that Brachypodium SWNs can act as switches that turn on secondary cell-wall synthesis and programmed cell death.
authors: Dpto. Fisiología Vegetal, Facultad de Biología, Universidad de Santiago, Santiago de Compostela, 15782, Spain.
authors: Elene R
authors: ER
authors: Valdivia
authors: María Teresa
authors: MT
authors: Herrera
authors: Cristina
authors: C
authors: Gianzo
authors: Javier
authors: J
authors: Fidalgo
authors: Gloria
authors: G
authors: Revilla
authors: Ignacio
authors: I
authors: Zarra
authors: Javier
authors: J
authors: Sampedro
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D058431:Brachypodium / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D014026:Nicotiana / Q000166:cytology / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D052584:Xylem / Q000235:genetics
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
references: Trends Plant Sci. 2010 Nov;15(11):625-32
references: 20833576
references: Genes Dev. 2005 Aug 15;19(16):1855-60
references: 16103214
references: Plant Signal Behav. 2011 Sep;6(9):1282-5
references: 21847026
references: Plant Mol Biol. 2000 Oct;44(3):245-53
references: 11199386
references: BMC Res Notes. 2011 Aug 19;4:302
references: 21851648
references: Plant Cell Physiol. 2009 Nov;50(11):1950-64
references: 19808805
references: Plant Physiol. 2007 Dec;145(4):1192-200
references: 17981986
references: Mol Plant. 2010 Nov;3(6):1087-103
references: 20935069
references: Mol Plant. 2012 Mar;5(2):297-303
references: 22138968
references: Curr Opin Plant Biol. 2008 Jun;11(3):301-7
references: 18434239
references: J Mol Evol. 2004 Apr;58(4):424-41
references: 15114421
references: Front Plant Sci. 2012 Apr 23;3:74
references: 22639662
references: Nucleic Acids Res. 2009 Apr;37(6):e45
references: 19237396
references: Plant J. 2011 May;66(4):579-90
references: 21284754
references: Annu Rev Plant Biol. 2010;61:263-89
references: 20192742
references: Plant J. 2008 Oct;56(2):303-315
references: 18573193
references: Nucleic Acids Res. 2002 May 1;30(9):e36
references: 11972351
references: Plant Physiol. 2003 Sep;133(1):73-83
references: 12970476
references: New Phytol. 2011 Jul;191(2):334-347
references: 21623796
references: J Exp Bot. 2012 Feb;63(3):1081-94
references: 22213814
references: Annu Rev Cell Dev Biol. 2010;26:605-37
references: 20590454
references: Nature. 2010 Feb 11;463(7282):763-8
references: 20148030
references: Front Plant Sci. 2011 Jul 01;2:23
references: 22639584
references: Plant Physiol. 2007 Dec;145(4):1183-91
references: 17965171
references: Plant J. 2011 Aug;67(3):499-512
references: 21649762
references: Plant Cell Rep. 2008 Mar;27(3):471-8
references: 17999063
references: Curr Opin Plant Biol. 2010 Jun;13(3):299-304
references: 20381410
references: Plant Physiol. 2003 Oct;133(2):462-9
references: 14555774
references: Plant Cell Physiol. 2011 Oct;52(10):1856-71
references: 21908441
references: BMC Plant Biol. 2008 Nov 07;8:112
references: 18992143
references: Methods Mol Biol. 2006;323:329-42
references: 16739588
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Plant Physiol. 2010 Jun;153(2):396-402
references: 20375115
references: Plant Cell. 2005 Nov;17(11):2993-3006
references: 16214898
references: Mol Plant. 2011 Mar;4(2):199-211
references: 21307367
references: Methods Mol Biol. 2006;323:263-73
references: 16739584
references: Plant Cell. 2010 Oct;22(10):3461-73
references: 20952636
references: Biotechniques. 1990 Apr;8(4):404-7
references: 2340178
references: Plant Cell. 2006 Nov;18(11):3158-70
references: 17114348