title: Structure of a low-melting-temperature anti-cholera toxin: llama V(H)H domain.
abstract: Variable heavy domains derived from the heavy-chain-only antibodies found in camelids (V(H)H domains) are known for their thermal stability. Here, the structure of A9, an anti-cholera toxin V(H)H domain (K(d) = 77 ± 5 nM) that has an unusually low melting temperature of 319.9 ± 1.6 K, is reported. The CDR3 residues of A9 form a β-hairpin that is directed away from the former V(H)-V(L) interfacial surface, exposing hydrophobic residues to the solvent. A DALI structural similarity search showed that this CDR3 conformation is uncommon.
authors: Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA. patricia.legler@nrl.navy.mil
authors: Patricia M
authors: PM
authors: Legler
authors: Dan
authors: D
authors: Zabetakis
authors: George P
authors: GP
authors: Anderson
authors: Anita
authors: A
authors: Lam
authors: Wim G J
authors: WG
authors: Hol
authors: Ellen R
authors: ER
authors: Goldman
mesh_terms: D000818:Animals; D002161:Camelids, New World / Q000276:immunology*; D002772:Cholera Toxin / Q000737:chemistry*; D002942:Circular Dichroism; D003080:Cold Temperature; D018360:Crystallography, X-Ray; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry*; D007135:Immunoglobulin Variable Region / Q000737:chemistry*; D020673:Protein Renaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D044366:Transition Temperature
chemical_list: D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D002772:Cholera Toxin
keywords: cholera toxin; single-domain antibodies; thermal stability
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