title: Serum fibrinogen alpha C-chain 5.9 kDa fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus.
abstract: OBJECTIVE
  Clinical application of biomarker candidates discovered by proteomic analysis is challenging. The purpose of this study was to standardize preanalytical conditions for measurement of serum levels of fibrinogen alpha C-chain 5.9 kDa fragment (FIC 5.9) and to test the diagnostic value of this peptide for detection of early hepatic fibrosis in patients with hepatitis C virus (HCV)-related chronic hepatitis.
  
  
  METHODS
  Serum FIC 5.9 levels were measured by a sandwich ELISA. Effects on the serum FIC 5.9 level of temperature, the time between venipuncture and serum separation, and the types of collection tubes used were examined. The diagnostic value of serum FIC 5.9 as an early indicator of hepatic fibrosis due to HCV was then assessed.
  
  
  RESULTS
  FIC 5.9 was produced in a time- and temperature-dependent manner after venipuncture. Abnormal FIC 5.9 values were found in 89.5% of FI stage patients. Receiver operating characteristic analyses confirmed the superiority of FIC 5.9 over other conventional markers for early detection of fibrosis.
  
  
  CONCLUSIONS
  The serum FIC 5.9 level may be an early indicator of hepatic fibrosis in HCV-related chronic liver diseases. This study provides an example of a pipeline from biomarker discovery by proteome analysis to assay optimization and preliminary clinical validation.
authors: Clinical Proteomics Research Center, Chiba University Hospital, Chiba, Japan.
authors: Kazuyuki
authors: K
authors: Sogawa
authors: Kenta
authors: K
authors: Noda
authors: Hiroshi
authors: H
authors: Umemura
authors: Masanori
authors: M
authors: Seimiya
authors: Takahisa
authors: T
authors: Kuga
authors: Takeshi
authors: T
authors: Tomonaga
authors: Motoi
authors: M
authors: Nishimura
authors: Fumihiko
authors: F
authors: Kanai
authors: Fumio
authors: F
authors: Imazeki
authors: Hirotaka
authors: H
authors: Takizawa
authors: Masato
authors: M
authors: Yoneda
authors: Atsushi
authors: A
authors: Nakajima
authors: Mikihiro
authors: M
authors: Tsutsumi
authors: Osamu
authors: O
authors: Yokosuka
authors: Fumio
authors: F
authors: Nomura
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D005340:Fibrinogen / Q000592:standards; D016174:Hepacivirus / Q000472:pathogenicity*; D019698:Hepatitis C, Chronic / Q000150:complications / Q000175:diagnosis; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood* / Q000592:standards; D012372:ROC Curve; D012016:Reference Values; D013696:Temperature; D013997:Time Factors
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; C098000:fibrinogen alphaC; D005340:Fibrinogen