title: Generation and analysis of a complete mutant set for the Arabidopsis FT/TFL1 family shows specific effects on thermo-sensitive flowering regulation.
abstract: The FLOWERING LOCUS T (FT)/TERMINAL FLOWER 1 (TFL1) family proteins play an important role in the regulation of flowering time. In the Arabidopsis thaliana genome, there are six genes in the FT/TFL1 family. To determine how these FT/TFL1 family genes contribute to the regulation of flowering time, this study generated a comprehensive set of mutants (sixty-three multiple mutants in all combinations) of the FT/TFL1 family genes and analysed their flowering times at 23 and 16°C under long-day conditions. The analysis confirmed that FT and TFL1 are major determinants of flowering time under long-day conditions. At 23 °C, ft-10 tsf-1 mft-2 showed the latest flowering, whereas tfl1-20 atc-2 bft-2 showed the earliest flowering. Flowering occurred in the sextuple mutants. Introduction of tsf-1 led to reduced sensitivity to ambient temperature change. Introduction of tfl1-20 caused a stronger effect in accelerating flowering time at 16 °C than at 23 °C. Overexpression of miR156 did not block flowering of sextuple mutants, suggesting that there is a pathway to induce flowering independent of the FT/TFL1 pathway and miR156 pathway. This study proposes that this mutant population will be useful in further investigation of the functions of the FT/TFL1 family genes in plant development.
authors: Creative Research Initiatives, Division of Life Sciences, Korea University, Anam dong 5 ga, Seongbuk-Gu, Seoul 136-701, Korea.
authors: Wanhui
authors: W
authors: Kim
authors: Tae Im
authors: TI
authors: Park
authors: Seong Jeon
authors: SJ
authors: Yoo
authors: A Rim
authors: AR
authors: Jun
authors: Ji Hoon
authors: JH
authors: Ahn
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D009154:Mutation; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism; D017440:Photoperiod; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; C418905:FT protein, Arabidopsis; D035683:MicroRNAs; C559759:Mirn156 microRNA, Arabidopsis; D049488:Phosphatidylethanolamine Binding Protein; D018749:RNA, Plant; C102864:TFL1 protein, Arabidopsis; C500577:TSF protein, Arabidopsis
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