title: Detection of a canine parvovirus type 2c with a non-coding mutation and its implications for molecular characterisation.
abstract: An epidemiological survey for canine parvovirus (CPV) was conducted by collecting 615 faecal samples from dogs with diarrhoea in different European countries. Molecular methods showed that CPV-2a was predominant in most countries, followed by CPV-2c and CPV-2b, whereas 30 strains were not characterised. By sequence analysis of the full-length VP2 gene, 20 of these viruses were characterised as CPV-2c mutants having the synonymous mutation A4061G in the probe-binding region that prevented correct strain characterisation. A real-time polymerase chain reaction (PCR) assay using a minor groove binder probe was able to recognise both mutant and classical CPV-2c strains. These results indicate that the emergence of CPVs with mutations affecting the oligonucleotide-binding region needs a continuous update of molecular diagnostic tools in order to detect efficiently those emerging strains.
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy. nicola.decaro@uniba.it
authors: Nicola
authors: N
authors: Decaro
authors: Costantina
authors: C
authors: Desario
authors: Francesca
authors: F
authors: Amorisco
authors: Michele
authors: M
authors: Losurdo
authors: Gabriella
authors: G
authors: Elia
authors: Antonio
authors: A
authors: Parisi
authors: Gianpiero
authors: G
authors: Ventrella
authors: Vito
authors: V
authors: Martella
authors: Canio
authors: C
authors: Buonavoglia
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D009154:Mutation; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary