title: Ureaplasma serovars & their antimicrobial susceptibility in patients of infertility & genital tract infections.
abstract: OBJECTIVE
  Ureaplasmas have been implicated in a variety of clinical conditions. However, only certain serovars of ureaplasmas are disease associated. Only a few classes of antimicrobial agents are available for the treatment of mycoplasmal infections in humans. Increase of resistance of genital mycoplasmas to antimicrobials has been reported worldwide. The aim of the present study was to determine the occurrence of Ureaplasma serovars in patients with infertility and genital tract infections with polymerase chain reaction (PCR)-based serotyping. The antimicrobial susceptibilities of Ureaplasma spp. and Mycoplasma hominis were also assessed to determine the most suitable treatment strategy.
  
  
  METHODS
  Sexually active adults (n=147) with symptoms of genital tract infections and 115 infertile women were enrolled. Endocervical swabs from women and urethral swabs from men were subjected to culture and multiplex PCR for detection of genital mycoplasmas. Serotyping of Ureaplasma was done by PCR and antimicrobial susceptibility to doxycycline, azithromycin, josamycin and ofloxacin was done by microbroth dilution method.
  
  
  RESULTS
  Ureaplasma was detected in 25.8 per cent patients with genital tract infections and 20.8 per cent in infertile women. Serovar 3/14 was the most frequent isolate followed by serovar 1 and serovar 6. The majority of Ureaplasma isolates were susceptible to doxycycline (91%) and josamycin (86%) followed by ofloxacin (77%) and azithromycin (71%). All the isolates of M. hominis were uniformly susceptible to doxycycline, josamycin and ofloxacin.
  
  
  CONCLUSIONS
  The predominance of Ureaplasma serovar 3/14 suggests their possible pathogenic role in genital tract infections and infertility. For empirical treatment, doxycycline could be the drug of choice for genital mycoplasmas.
authors: Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India. dhawanb@gmail.com
authors: Benu
authors: B
authors: Dhawan
authors: Neena
authors: N
authors: Malhotra
authors: Vishnubhatla
authors: V
authors: Sreenivas
authors: Jyoti
authors: J
authors: Rawre
authors: Neena
authors: N
authors: Khanna
authors: Rama
authors: R
authors: Chaudhry
authors: Suneeta
authors: S
authors: Mittal
mesh_terms: D000328:Adult; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D004318:Doxycycline / Q000494:pharmacology; D005260:Female; D006801:Humans; D007246:Infertility / Q000382:microbiology*; D015570:Josamycin / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D015242:Ofloxacin / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D060737:Reproductive Tract Infections / Q000382:microbiology*; D012703:Serotyping / Q000379:methods; D013045:Species Specificity; D014509:Ureaplasma / Q000187:drug effects* / Q000235:genetics*; D016869:Ureaplasma Infections / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000890:Anti-Infective Agents; D017963:Azithromycin; D015242:Ofloxacin; D015570:Josamycin; D004318:Doxycycline
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