title: Immobilization of Rhodococcus rhodochrous BX2 (an acetonitrile-degrading bacterium) with biofilm-forming bacteria for wastewater treatment.
abstract: In this study, a unique biofilm consisting of three bacterial strains with high biofilm-forming capability (Bacillus subtilis E2, E3, and N4) and an acetonitrile-degrading bacterium (Rhodococcus rhodochrous BX2) was established for acetonitrile-containing wastewater treatment. The results indicated that this biofilm exhibited strong resistance to acetonitrile loading shock and displayed a typical spatial and structural heterogeneity and completely depleted the initial concentration of acetonitrile (800mgL(-1)) within 24h in a moving-bed-biofilm reactor (MBBR) after operation for 30days. The immobilization of BX2 cells in the biofilm was confirmed by PCR-DGGE. It has been demonstrated that biofilm-forming bacteria can promote the immobilization of contaminant-degrading bacteria in the biofilms and can subsequently improve the degradation of contaminants in wastewater. This approach offers a novel strategy for enhancing biological oxidation of toxic pollutants in wastewater.
authors: College of Resource and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
authors: Chunyan
authors: C
authors: Li
authors: Yue
authors: Y
authors: Li
authors: Xiaosong
authors: X
authors: Cheng
authors: Liping
authors: L
authors: Feng
authors: Chuanwu
authors: C
authors: Xi
authors: Ying
authors: Y
authors: Zhang
mesh_terms: D000097:Acetonitriles / Q000302:isolation & purification / Q000378:metabolism*; D001412:Bacillus subtilis / Q000145:classification / Q000502:physiology*; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology; D018914:Cells, Immobilized; D012240:Rhodococcus / Q000145:classification / Q000502:physiology*; D013045:Species Specificity; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D000097:Acetonitriles; D062065:Wastewater; D014874:Water Pollutants, Chemical; C032159:acetonitrile