title: Histoenzymatic and morphometric analysis of muscle fiber type transformation during the postnatal development of the chronically food-deprived rat.
abstract: We analyze the effect of chronic undernourishment on extensor digitorum longus (EDL) muscle maturation in the rat. Cytochrome c oxidase (COX) and alkaline ATPase histoenzymatic techniques were used to determine the relative proportion of different fiber types (oxidative/glycolytic and type I, IIa/IId, or IIb, respectively) and their cross-sectional area in control and undernourished EDL muscles at several postnatal (PN) ages. From PN days 15 to 45, undernourished EDL muscles showed predominance of oxidative and type IIa/IId fibers, but from PN days 60 to 90, there were a larger proportion of oxidative fibers and an equal proportion of type IIa/IId and IIb fibers. Meanwhile, in adult stages (from PN days 130-365), the relative proportion of fiber types in control and undernourished EDL muscles showed no significant differences. In addition, from PN days 15 to 90, there was a significant reduction in the cross-sectional area of all fibers (slow: 13-53%; intermediate: 24-74%; fast: 9-80%) but no differences from PN days 130 to 365. It is suggested that chronic undernourishment affects the maturation of fast-type muscle fibers only at juvenile stages (from PN days 15-45) and the probable occurrence of adaptive mechanisms in muscle fibers, allowing adult rats to counterbalance the alterations provoked by chronic food deprivation.
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV, IPN, C.P.07360, Mexico City, Mexico.
authors: Azucena
authors: A
authors: Ruiz-Rosado
authors: Francisca
authors: F
authors: Fernández-Valverde
authors: Silvia
authors: S
authors: Mariscal-Tovar
authors: Cindy Xilonen
authors: CX
authors: Hinojosa-Rodriguez
authors: Jorge Arturo
authors: JA
authors: Hernández-Valencia
authors: Álvaro
authors: Á
authors: Anzueto-Rios
authors: José Carlos
authors: JC
authors: Guadarrama-Olmos
authors: Bertha
authors: B
authors: Segura-Alegría
authors: Ismael
authors: I
authors: Jiménez-Estrada
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D035002:Lower Extremity / Q000254:growth & development*; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000201:enzymology* / Q000502:physiology; D009929:Organ Size; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases
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