title: Mitochondrial genome of the eyeworm, Thelazia callipaeda (Nematoda: Spirurida), as the first representative from the family Thelaziidae.
abstract: Human thelaziosis is an underestimated parasitic disease caused by Thelazia species (Spirurida: Thelaziidae). The oriental eyeworm, Thelazia callipaeda, infects a range of mammalian definitive hosts, including canids, felids and humans. Although this zoonotic parasite is of socio-economic significance in Asian countries, its genetics, epidemiology and biology are poorly understood. Mitochondrial (mt) DNA is known to provide useful genetic markers to underpin fundamental investigations, but no mt genome had been characterized for any members of the family Thelaziidae. In the present study, we sequenced and characterized the mt genome of T. callipaeda. This AT-rich (74.6%) mt genome (13,668 bp) is circular and contains 12 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes, but lacks an atp8 gene. All protein-coding genes are transcribed in the same direction; the gene order is the same as those of Dirofilaria immitis and Setaria digitata (Onchocercidae), but distinct from Dracunculus medinensis (Dracunculidae) and Heliconema longissimum (Physalopteridae). Phylogenetic analyses of the concatenated amino acid sequence data for all 12 protein-coding genes by Bayesian inference (BI) showed that T. callipaeda (Thelaziidae) is related to the family Onchocercidae. This is the first mt genome of any member of the family Thelaziidae and should represent a new source of genetic markers for studying the epidemiology, ecology, population genetics and systematics of this parasite of humans and other mammals.
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, PR China.
authors: Guo-Hua
authors: GH
authors: Liu
authors: Robin B
authors: RB
authors: Gasser
authors: Domenico
authors: D
authors: Otranto
authors: Min-Jun
authors: MJ
authors: Xu
authors: Ji-Long
authors: JL
authors: Shen
authors: Namitha
authors: N
authors: Mohandas
authors: Dong-Hui
authors: DH
authors: Zhou
authors: Xing-Quan
authors: XQ
authors: Zhu
mesh_terms: D000818:Animals; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry* / Q000235:genetics*; D023061:Gene Order; D054629:Genome, Mitochondrial; D015801:Helminth Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D022661:RNA, Untranslated / Q000235:genetics; D017422:Sequence Analysis, DNA; D026801:Synteny; D013802:Thelazioidea / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004270:DNA, Circular; D018509:DNA, Helminth; D015801:Helminth Proteins; D022661:RNA, Untranslated
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