title: The promotion of a functional fibrosis in skeletal muscle with volumetric muscle loss injury following the transplantation of muscle-ECM.
abstract: Tissue engineering strategies that primarily use biological extracellular matrices (ECMs) with or without the inclusion of a stem or progenitor cell source are under development for the treatment of trauma resulting in the loss of a large volume of skeletal muscle (i.e., volumetric muscle loss; VML). The explicit goal is to restore functional capacity to the injured tissue by promoting generation of muscle fibers. In the current study, a syngeneic muscle-derived ECM (mECM) was transplanted in a rat tibialis anterior (TA) muscle VML model. Instead of muscle fiber generation a large fibrotic mass was produced by mECM transplantation out to six months post-injury. Surprisingly, recovery of one-third of the original functional deficit was still achieved by two months post-injury following mECM transplantation. These counterintuitive findings may be due, at least in part, to the ability of mECM to attenuate muscle damage in the remaining muscle as compared to non-repaired muscle. These findings point to a novel role of biological ECMs for the treatment of VML, wherein the remaining muscle mass is protected from prolonged overload injury.
authors: US Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, TX 78234, USA.
authors: Benjamin T
authors: BT
authors: Corona
authors: Xiaowu
authors: X
authors: Wu
authors: Catherine L
authors: CL
authors: Ward
authors: Jennifer S
authors: JS
authors: McDaniel
authors: Christopher R
authors: CR
authors: Rathbone
authors: Thomas J
authors: TJ
authors: Walters
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002921:Cicatrix / Q000473:pathology; D005109:Extracellular Matrix / Q000637:transplantation*; D005355:Fibrosis; D007537:Isometric Contraction; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D018482:Muscle, Skeletal / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology*; D009929:Organ Size; D009928:Organ Specificity; D051381:Rats; D011917:Rats, Inbred Lew; D012038:Regeneration; D014945:Wound Healing