title: [Somatic embryogenesis in in vitro culture of three larch species].
abstract: Embryogenic callus formation in different larch species from Siberia (Larix sibirica, L. gmelinii, and L. sukaczewii) was carried out on MSGm medium supplemented with growth regulators (2.4-D and BAP) and followed one and the same scheme: elongation of somatic cells and their asymmetric division with formation of initial and tube cells. The cells of embryo initial underwent sequential divisions and formed embryonic globules which caused the formation of somatic embryos. Somatic embryos became mature and germinated by addition of ABA and PEG into the medium. Long-term proliferating cell lines and regenerant plants were obtained in Sukachev larch and its hybrid with Siberian larch. The success of somatic embryogenesis depended on the genotype of the donor tree.
authors: I N
authors: IN
authors: Tret'iakova
authors: A V
authors: AV
authors: Barsukova
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D001593:Benzyl Compounds; D002455:Cell Division / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005838:Genotype; D018525:Germination / Q000187:drug effects / Q000502:physiology; D006822:Hybrid Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D028221:Larix / Q000187:drug effects / Q000196:embryology* / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology*; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011687:Purines; D012800:Siberia
chemical_list: D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D011092:Polyethylene Glycols; D000040:Abscisic Acid; C480551:benzylaminopurine; D007701:Kinetin