title: Catechins are not major components responsible for the beneficial effect of Camellia sinensis on the ovarian δ-ALA-D activity inhibited by cadmium.
abstract: Cadmium has been associated with a wide spectrum of deleterious effects on the reproductive tissues, including ovary. This investigation evaluated the protective role of Camellia sinensis (green, white and red teas) in the cadmium-induced inhibition of ovarian δ-aminolevulinate dehydratase (δ-ALA-D) activity in vitro and ex vivo. This study demonstrated that green and white teas restored the cow ovary δ-ALA-D activity inhibited by cadmium whereas red tea had no effect in vitro. In addition, green tea was able to restore enzyme activity inhibited after acute cadmium exposure in mice ovary. Teas infusions composition was assessed by HPLC in a quantitative assay for catechins, purine alkaloids and gallic acid as well as total polyphenol content. The greatest effect of green tea observed in vitro as well as the protective role presented in the ex vivo study could be attributed to the major content of phenols, but not catechins. In fact, catechins were not able to restore enzyme activity inhibited by cadmium, demonstrating that these compounds are not major components responsible for the beneficial effect of green tea observed in this study. This study demonstrated the helpful effect of green tea infusion in ameliorating a marker protein of cadmium intoxication in ovarian tissue.
authors: Laboratório de Biotecnologia da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal do Pampa, CEP 97500-970, Uruguaiana, RS, Brazil.
authors: Melina Bucco
authors: MB
authors: Soares
authors: Aryele Pinto
authors: AP
authors: Izaguirry
authors: Laura Musacchio
authors: LM
authors: Vargas
authors: Andreas Sebastian Loureiro
authors: AS
authors: Mendez
authors: Cristiano Chiapinotto
authors: CC
authors: Spiazzi
authors: Francielli Weber
authors: FW
authors: Santos
mesh_terms: D000470:Alkaloids / Q000032:analysis; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000032:analysis / Q000494:pharmacology*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005707:Gallic Acid / Q000032:analysis; D051379:Mice; D010053:Ovary / Q000187:drug effects* / Q000201:enzymology; D059808:Polyphenols / Q000032:analysis; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors*; D013662:Tea
chemical_list: D000470:Alkaloids; D059808:Polyphenols; D013662:Tea; D002104:Cadmium; D005707:Gallic Acid; D002392:Catechin; D000623:Porphobilinogen Synthase