title: Long noncoding RNAs regulate adipogenesis.
abstract: The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long noncoding RNA (lncRNA) during adipogenesis have not been explored. Here we profiled the transcriptome of primary brown and white adipocytes, preadipocytes, and cultured adipocytes and identified 175 lncRNAs that are specifically regulated during adipogenesis. Many lncRNAs are adipose-enriched, strongly induced during adipogenesis, and bound at their promoters by key transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (CEBPα). RNAi-mediated loss of function screens identified functional lncRNAs with varying impact on adipogenesis. Collectively, we have identified numerous lncRNAs that are functionally required for proper adipogenesis.
authors: Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
authors: Lei
authors: L
authors: Sun
authors: Loyal A
authors: LA
authors: Goff
authors: Cole
authors: C
authors: Trapnell
authors: Ryan
authors: R
authors: Alexander
authors: Kinyui Alice
authors: KA
authors: Lo
authors: Ezgi
authors: E
authors: Hacisuleyman
authors: Martin
authors: M
authors: Sauvageau
authors: Barbara
authors: B
authors: Tazon-Vega
authors: David R
authors: DR
authors: Kelley
authors: David G
authors: DG
authors: Hendrickson
authors: Bingbing
authors: B
authors: Yuan
authors: Manolis
authors: M
authors: Kellis
authors: Harvey F
authors: HF
authors: Lodish
authors: John L
authors: JL
authors: Rinn
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D007257:Information Theory; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D059467:Transcriptome / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding
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