title: X-Plate Technology: a new method for detecting fluconazole resistance in Candida species.
abstract: Candida species are responsible for many opportunistic fungal infections. Fluconazole is a well-tolerated antifungal drug, commonly used in the treatment of candidiasis. However, with fluconazole resistance ever increasing, rapid detection and antifungal susceptibility testing of Candida is imperative for proper patient treatment. This paper reports a cost-effective, simple and rapid chromogenic agar dilution method for simultaneous Candida species identification and fluconazole susceptibility testing. The results obtained by X-Plate Technology were in absolute concordance with standard microbroth dilution assays. Analysis of 1383 clinical patient samples with suspected vulvovaginal candidiasis revealed that this technology was able to detect and speciate the Candida isolate and determine the fluconazole susceptibility. The prevalence and susceptibility profiles of the clinical isolates using this method were highly similar to published reports using the microbroth dilution method.
authors: Femeris Women's Health Research Center, Medical Diagnostic Laboratories, LLC, A Member of Genesis Biotechnology Group, 2439 Kuser Road, Hamilton, NJ 08690, USA.
authors: Sean G
authors: SG
authors: Chadwick
authors: Femeris Women's Health Research Center, Medical Diagnostic Laboratories, LLC, A Member of Genesis Biotechnology Group, 2439 Kuser Road, Hamilton, NJ 08690, USA.
authors: Jessica A
authors: JA
authors: Schuyler
authors: Femeris Women's Health Research Center, Medical Diagnostic Laboratories, LLC, A Member of Genesis Biotechnology Group, 2439 Kuser Road, Hamilton, NJ 08690, USA.
authors: John-Paul
authors: JP
authors: Vermitsky
authors: Femeris Women's Health Research Center, Medical Diagnostic Laboratories, LLC, A Member of Genesis Biotechnology Group, 2439 Kuser Road, Hamilton, NJ 08690, USA.
authors: Martin E
authors: ME
authors: Adelson
authors: Femeris Women's Health Research Center, Medical Diagnostic Laboratories, LLC, A Member of Genesis Biotechnology Group, 2439 Kuser Road, Hamilton, NJ 08690, USA.
authors: Eli
authors: E
authors: Mordechai
authors: Femeris Women's Health Research Center, Medical Diagnostic Laboratories, LLC, A Member of Genesis Biotechnology Group, 2439 Kuser Road, Hamilton, NJ 08690, USA.
authors: Scott E
authors: SE
authors: Gygax
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects*; D002863:Chromogenic Compounds / Q000737:chemistry; D015169:Colony Count, Microbial; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods* / Q000592:standards
chemical_list: D000935:Antifungal Agents; D002863:Chromogenic Compounds; D003470:Culture Media; D015725:Fluconazole