title: A universal fluid cell for the imaging of biological specimens in the atomic force microscope.
abstract: Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set-up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells.
authors: Laboratoire de Physique de la Matière Vivante, EPFL, CH-1015, Lausanne, Switzerland. sandor.kasas@epfl.ch
authors: Sandor
authors: S
authors: Kasas
authors: Ksenja
authors: K
authors: Radotic
authors: Giovanni
authors: G
authors: Longo
authors: Bashkar
authors: B
authors: Saha
authors: Livan
authors: L
authors: Alonso-Sarduy
authors: Giovanni
authors: G
authors: Dietler
authors: Charles
authors: C
authors: Roduit
mesh_terms: D017360:Arabidopsis / Q000166:cytology*; D004926:Escherichia coli / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods