title: Characterization of three H5N5 and one H5N8 highly pathogenic avian influenza viruses in China.
abstract: One H5N8 and three H5N5 highly pathogenic avian influenza (HPAI) viruses which derived their HA genes from the Asian H5N1 lineage were isolated from poultry during 2009-2010 in mainland China. Pathogenicity studies showed that these viruses were all highly virulent to chickens, while they varied from moderate to high virulence in mice and from mild to intermediate virulence in mallards. Phylogenetic analyses showed that these viruses were reassortants bearing the H5N1 backbone while acquiring PB1, NP and NA genes from unidentified non-H5N1 viruses, and had developed into three distinct genotypes (B-D). Molecular characterization indicated that all these viruses might resist to antiviral agents. Our findings highlight the emergence and development of HPAI H5 viruses of other NA subtypes in H5N1 endemic areas and their potential threat to poultry industry and public health.
authors: Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
authors: Kunkun
authors: K
authors: Zhao
authors: Min
authors: M
authors: Gu
authors: Lei
authors: L
authors: Zhong
authors: Zhiqiang
authors: Z
authors: Duan
authors: Yan
authors: Y
authors: Zhang
authors: Yanmei
authors: Y
authors: Zhu
authors: Guo
authors: G
authors: Zhao
authors: Mingjun
authors: M
authors: Zhao
authors: Zhaoyang
authors: Z
authors: Chen
authors: Shunlin
authors: S
authors: Hu
authors: Wenbo
authors: W
authors: Liu
authors: Xiaowen
authors: X
authors: Liu
authors: Daxin
authors: D
authors: Peng
authors: Xiufan
authors: X
authors: Liu
mesh_terms: D000818:Animals; D002645:Chickens; D002681:China; D004372:Ducks; D005260:Female; D005838:Genotype; D006389:Hemagglutinins, Viral / Q000235:genetics; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000235:genetics; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014758:Viral Core Proteins / Q000235:genetics; D019562:Viral Load; D014764:Viral Proteins; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
chemical_list: D006389:Hemagglutinins, Viral; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1