title: In vivo detection of hemoglobin oxygen saturation and carboxyhemoglobin saturation with multiwavelength photoacoustic microscopy.
abstract: A method for noninvasively detecting hemoglobin oxygen saturation (SO2) and carboxyhemoglobin saturation (SCO) in subcutaneous microvasculature with multiwavelength photoacoustic microscopy is presented. Blood samples mixed with different concentrations of carboxyhemoglobin were used to test the feasibility and accuracy of photoacoustic microscopy compared with the blood-gas analyzer. Moreover, fixed-point detection of SO2 and SCO in mouse ear was obtained, and the changes from normoxia to carbon monoxide hypoxia were dynamically monitored in vivo. Experimental results demonstrate that multiwavelength photoacoustic microscopy can detect SO2 and SCO, which has future potential clinical applications.
authors: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors: Zhongjiang
authors: Z
authors: Chen
authors: Sihua
authors: S
authors: Yang
authors: Da
authors: D
authors: Xing
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D002263:Carboxyhemoglobin / Q000032:analysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006454:Hemoglobins / Q000032:analysis*; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D010092:Oximetry / Q000295:instrumentation*; D061088:Photoacoustic Techniques / Q000295:instrumentation*
chemical_list: D006454:Hemoglobins; D002263:Carboxyhemoglobin