title: Lipid-coated gold nanoparticles promote lamellar body formation in A549 cells.
abstract: Gold nanoparticles (GNPs) have been applied as diagnostic and therapeutic agents because they can be targeted, localized, and be heated to cause cell death. However, their use has been limited by their relatively low biocompatibility. In this work, we coated the GNPs' surface by a biocompatible phospholipid bilayer composed of 1-stearoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (SOPG). We tested their interaction with A549 cells to investigate their uptake and intracellular fate as well as the response of the cells to the presence of the GNPs. We used flow cytometry and confocal microscopy to show that the SOPG coated GNPs were readily taken up by the A549 cells. Transmission electron microscopy (TEM) images and fluorescence images further showed that the number of granular structures in the cells was increased following exposure to the lipid coated GNPs. Co-localization experiments demonstrated that SOPG coated GNPs localize in acidic compartments in a time dependent manner and that the number of these increase as the cells are exposed to the GNPs suggesting that they induce formation of lamellar bodies (LBs) which in A549 cells in turn can serve as a means of exporting the GNPs.
authors: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors: Meijing
authors: M
authors: Wang
authors: Nils O
authors: NO
authors: Petersen
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D005089:Exocytosis / Q000502:physiology; D005434:Flow Cytometry; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D057146:Multivesicular Bodies / Q000378:metabolism* / Q000648:ultrastructure; D022142:Secretory Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D008055:Lipids; D006046:Gold