title: Evaluation of the Cepheid Xpert GBS assay for rapid detection of group B Streptococci in amniotic fluids from pregnant women with premature rupture of membranes.
abstract: The Xpert GBS real-time PCR assay for the detection of group B streptococci (GBS) in antepartum screening samples was evaluated on amniotic fluid samples collected from 139 women with premature rupture of membrane at term. When any intrapartum positive result from the Xpert GBS or culture was considered a true positive, the sensitivities of the Xpert GBS and culture were 92.3% and 84.6%, respectively. This assay could enhance exact identification of candidates for intrapartum antibiotic prophylaxis.
authors: Service de Bactériologie-Hygiène, Hopital Antoine Béclère, APHP, Université Paris Sud, Clamart, France.
authors: Nadege
authors: N
authors: Bourgeois-Nicolaos
authors: Anne-Gael
authors: AG
authors: Cordier
authors: Christelle
authors: C
authors: Guillet-Caruba
authors: François
authors: F
authors: Casanova
authors: Alexandra
authors: A
authors: Benachi
authors: Florence
authors: F
authors: Doucet-Populaire
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D005322:Premature Rupture of Fetal Membranes; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis*; D013292:Streptococcus agalactiae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
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