title: The structure of the FnI-EGF-like tandem domain of coagulation factor XII solved using SIRAS.
abstract: Coagulation factor XII (FXII) is a key protein in the intrinsic coagulation and kallikrein-kinin pathways. It has been found that negative surfaces and amyloids, such as Aβ fibrils, can activate FXII. Additionally, it has been suggested that FXII simulates cells and that it plays an important role in thrombosis. To date, no structural data on FXII have been deposited, which makes it difficult to support any hypothesis on the mechanism of FXII function. The crystal structure of the FnI-EGF-like tandem domain of FXII presented here was solved using experimental phases. To determine the phases, a SIRAS approach was used with a native and a holmium chloride-soaked data set. The holmium cluster was coordinated by the C-terminal tails of two symmetry-related molecules. Another observation was that the FnI domain was much more ordered than the EGF-like domain owing to crystal packing. Furthermore, the structure shows the same domain orientation as the homologous FnI-EGF-like tandem domain of tPA. The plausibility of several proposed interactions of these domains of FXII is discussed. Based on this FXII FnI-EGF-like structure, it could be possible that FXII binding to amyloid and negatively charged surfaces is mediated via this part of FXII.
authors: Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors: D X
authors: DX
authors: Beringer
authors: L M J
authors: LM
authors: Kroon-Batenburg
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D004815:Epidermal Growth Factor / Q000737:chemistry*; D066246:ErbB Receptors / Q000378:metabolism; D005174:Factor XII / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D006695:Holmium; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D055672:Static Electricity; D013816:Thermodynamics; D010959:Tissue Plasminogen Activator / Q000737:chemistry
chemical_list: D005353:Fibronectins; D004815:Epidermal Growth Factor; D005174:Factor XII; D066246:ErbB Receptors; D010959:Tissue Plasminogen Activator; D006695:Holmium
keywords: EGF-like domain; FXII; Hageman factor; experimental phasing; fibronectin type I domain
references: Eur J Biochem. 1996 May 15;238(1):240-9
references: 8665943
references: Brain Res. 1994 Aug 22;654(2):234-40
references: 7987673
references: Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
references: 20057044
references: Acta Crystallogr B. 2002 Jun;58(Pt 3 Pt 1):380-8
references: 12037359
references: Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):493-9
references: 17372354
references: Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
references: 20383002
references: Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
references: 20124692
references: Science. 1991 Dec 13;254(5038):1608-15
references: 1721241
references: J Biol Chem. 1985 Nov 5;260(25):13666-76
references: 3877053
references: Structure. 2009 Mar 11;17(3):472-81
references: 19278661
references: Proc Natl Acad Sci U S A. 1991 May 15;88(10):4382-5
references: 1852005
references: J Mol Biol. 2011 Jan 14;405(2):341-8
references: 21059358
references: Biochemistry. 1986 Oct 21;25(21):6688-94
references: 3491625
references: J Biol Chem. 1987 Jul 25;262(21):10140-5
references: 2440859
references: Biochemistry. 2011 Jun 21;50(24):5477-86
references: 21598903
references: J Exp Med. 1981 Mar 1;153(3):665-76
references: 7252410
references: Nature. 2003 May 8;423(6936):177-81
references: 12736686
references: Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):582-601
references: 19465773
references: J Biol Chem. 1989 Jul 5;264(19):11497-502
references: 2472397
references: Blood. 2009 Jul 23;114(4):881-90
references: 19372258
references: Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4195-200
references: 19251642
references: Biochemistry. 1998 Dec 22;37(51):17965-76
references: 9922165
references: Blood. 1996 Mar 15;87(6):2337-44
references: 8630396
references: Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):2174-9
references: 8700904
references: Cell. 2002 Sep 20;110(6):775-87
references: 12297050
references: Cell. 2009 Dec 11;139(6):1143-56
references: 20005807
references: Nature. 2004 Jan 22;427(6972):320-5
references: 14737160
references: Cell. 2002 Sep 20;110(6):763-73
references: 12297049
references: Structure. 1998 Apr 15;6(4):477-89
references: 9562557
references: Alzheimer Dis Assoc Disord. 1998 Jun;12(2):102-8
references: 9651139
references: J Clin Invest. 2008 Sep;118(9):3208-18
references: 18725990
references: Nat Protoc. 2008;3(7):1171-9
references: 18600222
references: J Biomed Mater Res. 1996 May;31(1):63-70
references: 8731150
references: J Exp Med. 2005 Jul 18;202(2):271-81
references: 16009717
references: Amyloid. 2008 Sep;15(3):166-80
references: 18925455
references: Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12254-8
references: 18713862
references: Thromb Haemost. 2000 Dec;84(6):1057-65
references: 11154114
references: J Mol Biol. 2007 Sep 21;372(3):774-97
references: 17681537
references: J Mol Biol. 2011 May 6;408(3):529-40
references: 21376731
references: Biomaterials. 2006 Aug;27(24):4325-32
references: 16644008
references: Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41
references: 11517324
references: Structure. 1995 Aug 15;3(8):823-33
references: 7582899
references: Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67
references: 22505256
references: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):903-8
references: 16410357
references: Neurobiol Aging. 2001 Jan-Feb;22(1):63-9
references: 11164277
references: J Biol Chem. 1992 Feb 5;267(4):2274-81
references: 1733935
references: Blood. 2010 Jun 17;115(24):5111-20
references: 20228268
references: Thromb Res. 1992 Sep 15;67(6):665-76
references: 1279836