title: Cross-reactivity of secondary antibodies against African rodents and application for sero-surveillance.
abstract: A total of 466 rodents were captured in the Republic of Zambia from 2006 to 2010. Based on morphological observations and phylogenetic analyses of mitochondrial gene sequences, rodents were divided into 10 groups consisting of 39 Rattus rodents, 263 multimammate rats, 18 other Murinae rodents, 95 gerbils, 11 pouched mice, 1 giant-pouched rat, 38 fat mice and 1 dormouse. Rodent antibodies except that from Rattus were examined for their cross-reactivity to commercially available antibody detection reagents. Anti-mouse immunoglobulin G (IgG) was most cross-reactive to heterologous antibodies including multimammate rat, gerbil, pouched mouse and fat mouse. Thus, anti-mouse IgG would be a useful detection tool in serological examination of the Zambian rodent population. Preliminary sero-surveillance for plague, leptospirosis and hantavirus infection was performed by ELISA.
authors: Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan. inaka@czc.hokudai.ac.jp
authors: Ichiro
authors: I
authors: Nakamura
authors: Bernard Mudenda
authors: BM
authors: Hang'ombe
authors: Hirofumi
authors: H
authors: Sawa
authors: Shintaro
authors: S
authors: Kobayashi
authors: Yasuko
authors: Y
authors: Orba
authors: Akihiro
authors: A
authors: Ishii
authors: Yuka
authors: Y
authors: Thomas
authors: Rie
authors: R
authors: Isozumi
authors: Kumiko
authors: K
authors: Yoshimatsu
authors: Aaron S
authors: AS
authors: Mweene
authors: Ayato
authors: A
authors: Takada
authors: Chihiro
authors: C
authors: Sugimoto
authors: Jiro
authors: J
authors: Arikawa
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D012376:Rodent Diseases / Q000097:blood / Q000276:immunology*; D012377:Rodentia / Q000145:classification / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D015024:Zambia
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D007074:Immunoglobulin G