title: Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803.
abstract: We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steady-state fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.
authors: Department of Biology, Washington University, St. Louis, Missouri, USA.
authors: Brian P
authors: BP
authors: Landry
authors: Jana
authors: J
authors: Stöckel
authors: Himadri B
authors: HB
authors: Pakrasi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D029968:Escherichia coli Proteins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D059748:Proteolysis; D012329:RNA, Bacterial / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism; D046939:Synechocystis / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D016601:RNA-Binding Proteins; C430328:small protein B; C477700:sspB protein, E coli
references: Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10584-9
references: 11535833
references: Bioinformatics. 2007 Nov 1;23(21):2947-8
references: 17846036
references: Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6156-61
references: 18427117
references: Methods Enzymol. 1988;167:747-54
references: 3148842
references: Nucleic Acids Res. 2010 May;38(8):2577-93
references: 20236988
references: Appl Environ Microbiol. 1998 Jun;64(6):2240-6
references: 9603842
references: PLoS One. 2008;3(11):e3647
references: 18985154
references: Nucleic Acids Res. 2004 Jan 02;32(1):11-6
references: 14704338
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D115-22
references: 22194640
references: J Phycol. 1968 Mar;4(1):1-4
references: 27067764
references: Mol Cell. 2006 Jun 9;22(5):701-7
references: 16762842
references: Nature. 2005 May 5;435(7038):118-22
references: 15875027
references: Mol Gen Genet. 2001 Feb;264(6):924-30
references: 11254140
references: J Bacteriol. 1994 Nov;176(21):6764-8
references: 7961432