title: Crystallization and preliminary X-ray characterization of an NAD(P)-dependent butanol dehydrogenase A from Geobacillus thermodenitrificans NG80-2.
abstract: Geobacillus thermodenitrificans NG80-2 encodes two long-chain NAD(P)-dependent alcohol dehydrogenases, gtADH1 and gtADH2, in the terminal oxidation pathway of long-chain n-alkanes for the conversion of long-chain alkyl alcohols to their corresponding aldehydes. Both gtADH1 and gtADH2 are thermostable enzymes and oxidize long-chain alkyl alcohols up to at least C(30). In order to understand the structural basis for their role in long-chain alkane degradation, we have crystallized gtADH2. Single, colourless crystals were obtained from a recombinant preparation of ADH2 overexpressed in Escherichia coli. The crystals belong to space group C222(1), with unit-cell parameters a = 56.0, b = 99.6, c = 123.1 Å. Diffraction data were collected in-house to 1.79 Å resolution. The crystals contain one monomer in the asymmetric unit, with a V(M) value of 2.17 Å(3) Da(-1) and an estimated solvent content of 43%.
authors: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin 300071, People's Republic of China.
authors: Yurui
authors: Y
authors: Ji
authors: Guannan
authors: G
authors: Mao
authors: Yingying
authors: Y
authors: Wang
authors: Mark
authors: M
authors: Bartlam
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D056506:Geobacillus / Q000201:enzymology*; D009249:NADP / Q000378:metabolism*; D055503:Protein Multimerization; D012996:Solutions
chemical_list: D012996:Solutions; D009249:NADP; D000429:Alcohol Oxidoreductases; C060833:butanol dehydrogenase
keywords: Geobacillus thermodenitrificans NG80-2; alcohol dehydrogenase; long-chain alkane degradation
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