title: PF2401-SF, standardized fraction of Salvia miltiorrhiza, induces apoptosis of activated hepatic stellate cells in vitro and in vivo.
abstract: During the course of our attempts to develop a potential herbal medicine, we had previously prepared PF2401-SF, a standardized fraction of S. miltiorrhiza, and reported its hepatoprotective activity in vitro as well as in vivo. Since apoptosis of activated hepatic stellate cells (HSCs) is a well-accepted anti-fibrotic strategy, in this study, we investigated the direct effect of PF2401-SF on t-HSC/Cl-6 cells in vitro and on CCl₄-induced liver injury in vivo. We evaluated the activation and cleavage of hallmarkers of apoptosis, namely, caspase 3, 8, 9 and PARP. Upregulation of the pro-apoptotic Bax protein and downregulation of the anti-apoptotic Bcl2 protein were also analyzed. Furthermore, in the PF2401-SF treated rats, apoptosis induction of activated HSCs was demonstrated by reduced distribution of α-SMA-positive cells and the presence of high number of TUNEL-positive cells in vivo. Our data suggest that PF2401-SF can mediate HSCs apoptosis induction, and may be a potential herbal medicine for the treatment of liver fibrosis.
authors: Department of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University, Iksan, Jeonbuk 570-749, Korea.
authors: Daya Ram
authors: DR
authors: Parajuli
authors: Eun-Jeon
authors: EJ
authors: Park
authors: Xian-Hua
authors: XH
authors: Che
authors: Wen-Yi
authors: WY
authors: Jiang
authors: Youn-Chul
authors: YC
authors: Kim
authors: Dong Hwan
authors: DH
authors: Sohn
authors: Sung Hee
authors: SH
authors: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002251:Carbon Tetrachloride; D005591:Chemical Fractionation; D003124:Colorimetry; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D008099:Liver; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D051017:Apoptosis Regulatory Proteins; C582136:PF2401-SF; D010936:Plant Extracts; D002251:Carbon Tetrachloride
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