title: Cell type-specific genes show striking and distinct patterns of spatial expression in the mouse brain.
abstract: To characterize gene expression patterns in the regional subdivisions of the mammalian brain, we integrated spatial gene expression patterns from the Allen Brain Atlas for the adult mouse with panels of cell type-specific genes for neurons, astrocytes, and oligodendrocytes from previously published transcriptome profiling experiments. We found that the combined spatial expression patterns of 170 neuron-specific transcripts revealed strikingly clear and symmetrical signatures for most of the brain's major subdivisions. Moreover, the brain expression spatial signatures correspond to anatomical structures and may even reflect developmental ontogeny. Spatial expression profiles of astrocyte- and oligodendrocyte-specific genes also revealed regional differences; these defined fewer regions and were less distinct but still symmetrical in the coronal plane. Follow-up analysis suggested that region-based clustering of neuron-specific genes was related to (i) a combination of individual genes with restricted expression patterns, (ii) region-specific differences in the relative expression of functional groups of genes, and (iii) regional differences in neuronal density. Products from some of these neuron-specific genes are present in peripheral blood, raising the possibility that they could reflect the activities of disease- or injury-perturbed networks and collectively function as biomarkers for clinical disease diagnostics.
authors: Institute for Genomic Biology and Department of Computer Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors: Younhee
authors: Y
authors: Ko
authors: Seth A
authors: SA
authors: Ament
authors: James A
authors: JA
authors: Eddy
authors: Juan
authors: J
authors: Caballero
authors: John C
authors: JC
authors: Earls
authors: Leroy
authors: L
authors: Hood
authors: Nathan D
authors: ND
authors: Price
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D051379:Mice; D009474:Neurons / Q000378:metabolism; D059467:Transcriptome
chemical_list: D015415:Biomarkers
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