title: MicroRNA-140 promotes adipocyte lineage commitment of C3H10T1/2 pluripotent stem cells via targeting osteopetrosis-associated transmembrane protein 1.
abstract: BMP4 has been shown to induce C3H10T1/2 pluripotent stem cells to commit to adipocyte lineage. In addition to several proteins identified, microRNAs also play a critical role in the process. In this study, we identified microRNA-140 (miR-140) as a direct downstream component of the BMP4 signaling pathway during the commitment of C3H10T1/2 cells to adipocyte lineage. Overexpression of miR-140 in C3H10T1/2 cells promoted commitment, whereas knockdown of its expression led to impairment. Additional studies indicated that Ostm1 is a bona fide target of miR-140, which is significantly decreased during commitment, and Ostm1 was also demonstrated to function as an anti-adipogenic factor.
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China; Institute of Stem Cell Research and Regenerative Medicine, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
authors: Yuan
authors: Y
authors: Liu
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China.
authors: Zhi-Chun
authors: ZC
authors: Zhang
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China; Institute of Stem Cell Research and Regenerative Medicine, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
authors: Shu-Wen
authors: SW
authors: Qian
authors: Institute of Stem Cell Research and Regenerative Medicine, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
authors: You-You
authors: YY
authors: Zhang
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China; Institute of Stem Cell Research and Regenerative Medicine, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
authors: Hai-Yan
authors: HY
authors: Huang
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China.
authors: Yan
authors: Y
authors: Tang
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China.
authors: Liang
authors: L
authors: Guo
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China; Institute of Stem Cell Research and Regenerative Medicine, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China. Electronic address: lixi@shmu.edu.cn.
authors: Xi
authors: X
authors: Li
authors: Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, China; Institute of Stem Cell Research and Regenerative Medicine, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China. Electronic address: qqtang@shmu.edu.cn.
authors: Qi-Qun
authors: QQ
authors: Tang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D055415:Bone Morphogenetic Protein 4 / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000502:physiology*; D034622:RNA Interference; D015533:Transcriptional Activation
chemical_list: C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; C547828:MIRN140 microRNA, mouse; D008565:Membrane Proteins; D035683:MicroRNAs; C509143:OSTM1 protein, mouse
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