title: Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism.
abstract: Formation of a stable polarity axis underlies numerous biological processes. Here, using high-resolution imaging and complementary mathematical modeling we find that cell polarity can be established via the spatial coordination of opposing membrane trafficking activities: endocytosis and exocytosis. During polarity establishment in budding yeast, these antagonistic processes become apposed. Endocytic vesicles corral a central exocytic zone, tightening it to a vertex that establishes the polarity axis for the ensuing cell cycle. Concomitantly, the endocytic system reaches an equilibrium where internalization events occur at a constant frequency. Endocytic mutants that failed to initiate periodic internalization events within the corral displayed wide, unstable polarity axes. These results, predicted by in silico modeling and verified by high resolution in vivo studies, identify a requirement for endocytic corralling during robust polarity establishment.
authors: Dynamics of Cell Growth and Division, Institut de Biologie Cellulaire et de Génétique, Centre National de la Recherche Scientifique, UMR 5095, 33000 Bordeaux, France.
authors: Mini
authors: M
authors: Jose
authors: Sylvain
authors: S
authors: Tollis
authors: Deepak
authors: D
authors: Nair
authors: Jean-Baptiste
authors: JB
authors: Sibarita
authors: Derek
authors: D
authors: McCusker
mesh_terms: D016764:Cell Polarity / Q000502:physiology*; D003198:Computer Simulation; D004705:Endocytosis / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology; D008954:Models, Biological; D021381:Protein Transport; D012440:Saccharomyces / Q000166:cytology* / Q000378:metabolism; D013269:Stochastic Processes
references: Trends Cell Biol. 2012 Jan;22(1):1-13
references: 22018597
references: J Cell Biol. 1984 Mar;98(3):922-33
references: 6365930
references: J Microsc. 2007 Mar;225(Pt 3):214-28
references: 17371444
references: PLoS Biol. 2009 Sep;7(9):e1000204
references: 19787029
references: Science. 1997 Apr 4;276(5309):118-22
references: 9082982
references: EMBO J. 2002 Apr 2;21(7):1565-76
references: 11927541
references: PLoS Comput Biol. 2010 Sep 09;6(9):
references: 20838575
references: Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3517-21
references: 20133592
references: EMBO J. 2002 Feb 15;21(4):602-14
references: 11847108
references: J Cell Biol. 1998 Dec 28;143(7):1931-45
references: 9864365
references: Dev Cell. 2005 Jul;9(1):87-98
references: 15992543
references: Nat Rev Neurosci. 2011 Mar;12(3):127-38
references: 21304549
references: Phys Rev E Stat Nonlin Soft Matter Phys. 2005 Apr;71(4 Pt 1):041103
references: 15903653
references: Curr Biol. 2003 Sep 16;13(18):1636-40
references: 13678596
references: Eukaryot Cell. 2010 Oct;9(10):1504-18
references: 20693304
references: Cell. 2009 Nov 13;139(4):731-43
references: 19914166
references: J Cell Biol. 1993 Aug;122(3):635-44
references: 8335689
references: Nat Protoc. 2011 Nov 17;6(12):1953-68
references: 22094731
references: PLoS One. 2012;7(2):e30977
references: 22383986
references: PLoS Comput Biol. 2006 Sep 8;2(9):e117
references: 16965175
references: Curr Biol. 2011 Feb 8;21(3):184-94
references: 21277209
references: Cell. 2007 Apr 20;129(2):411-22
references: 17448998
references: Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):751-6
references: 11805329
references: J Cell Biol. 2004 Dec 6;167(5):889-901
references: 15583031
references: Science. 2003 Feb 21;299(5610):1231-5
references: 12560471
references: FEBS Lett. 2008 Apr 30;582(10):1437-43
references: 18381072
references: J Cell Sci. 2011 May 1;124(Pt 9):1533-41
references: 21486946
references: J Cell Biol. 2008 Jan 14;180(1):145-58
references: 18195105
references: Mol Biol Cell. 2011 Mar 1;22(5):624-33
references: 21209323
references: FEBS Lett. 1999 Sep 3;457(3):452-4
references: 10471827
references: J Cell Biol. 1999 Nov 15;147(4):791-808
references: 10562281
references: J Cell Sci. 2006 Mar 1;119(Pt 5):876-88
references: 16478783
references: Mol Biol Cell. 2012 May;23(10):1998-2013
references: 22438587
references: J Biol Chem. 2009 Nov 13;284(46):32158-66
references: 19759010
references: Mol Biol Cell. 2012 Feb;23(4):657-68
references: 22190733
references: Dev Cell. 2009 Dec;17(6):823-35
references: 20059952
references: Cell. 2005 Oct 21;123(2):305-20
references: 16239147
references: Curr Biol. 2002 Sep 17;12(18):1623-7
references: 12372257
references: Cell. 2003 Nov 14;115(4):475-87
references: 14622601
references: Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14593-8
references: 16199522
references: Trends Plant Sci. 2009 Jun;14(6):318-27
references: 19446491
references: Mol Biol Cell. 2010 Apr 1;21(7):1237-52
references: 20147449
references: Mol Biol Cell. 2012 Sep;23(17):3336-47
references: 22767578
references: Mol Biol Cell. 2005 Mar;16(3):1500-12
references: 15647373
references: Biophys J. 2005 Oct;89(4):2458-72
references: 16055544
references: J Biol Chem. 2001 Dec 14;276(50):46745-50
references: 11595741