title: Recovery of fat snook, Centropomus parallelus (Teleostei: Perciformes) after subchronic exposure to copper.
abstract: We studied the recovery of juvenile fat snook (Centropomus parallelus) after subchronic exposure to different concentrations of copper. Healthy juveniles (1.98 g) were exposed to 25 or 50 μg Cu/L for 30 days (12 replicates with 5 fish in each one), and recovery was observed at 0, 4, 10, and 30 days after exposure (3 replicates with 5 fish in each one). Copper genotoxicity in exposed individuals was observed using a micronucleus assay, and recovery was not observed even 30 days post-exposure. Copper accumulation was observed in fish exposed to 25 or 50 μg/L of copper in the gills (14.4 and 34.4 μg/g, respectively) and muscle (5.7 and 5.5 μg/g, respectively), and a return to normal copper levels (6.0 μg/g for gills and 2.5 μg/g for muscle) was observed 4 and 30 days post-exposure in the gills and muscle tissues, respectively. Glutathione S-transferase (GST) was 80% inhibited in individuals exposed to copper and returned to normal levels for fish exposed to basal concentrations within 10 days. Although copper accumulation in tissues dispersed 30 days post-exposure, no recovery from genotoxicity was observed during this time. Thirty days was not enough to recover juvenile fat snook following subchronic exposure to copper.
authors: Vila Velha University, Laboratory of Applied Ichthyology, Vila Velha, ES, Brazil.
authors: Rodrigo Nunes
authors: RN
authors: Oss
authors: Vinícius Dadalto
authors: VD
authors: Baroni
authors: Rafael Mendonça
authors: RM
authors: Duarte
authors: Adalberto Luis
authors: AL
authors: Val
authors: Vera Maria Fonseca Almeida
authors: VM
authors: Val
authors: Levy Carvalho
authors: LC
authors: Gomes
mesh_terms: D000367:Age Factors; D000818:Animals; D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005880:Gills / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism; D015162:Micronucleus Tests; D009132:Muscles / Q000187:drug effects; D010473:Perciformes / Q000502:physiology*; D014018:Tissue Distribution; D059550:Toxicity Tests, Subchronic; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper; D005982:Glutathione Transferase