title: Freeze-drying as sample preparation for micellar electrokinetic capillary chromatography-electrochemical separations of neurochemicals in Drosophila brains.
abstract: Micellar electrokinetic capillary chromatography with electrochemical detection has been used to quantify biogenic amines in freeze-dried brains of Drosophila melanogaster. Freeze-drying samples offers a way to preserve the biological sample while making dissection of these tiny samples easier and faster. Fly samples were extracted in cold acetone and dried in a rotary evaporator. Extraction and drying times were optimized in order to avoid contamination by red pigment from the fly eyes and still have intact brain structures. Single freeze-dried fly brain samples were found to produce representative electropherograms as a single hand-dissected brain sample. With utilization of the faster dissection time that freeze-drying affords, the number of brains in a fixed homogenate volume can be increased to concentrate the sample. Thus, concentrated brain samples containing five or fifteen preserved brains were analyzed for their neurotransmitter content, and four analytes; N-acetyloctopamine, N-acetylserotonin, N-acetyltyramine, and N-acetyldopamine were found to correspond well with previously reported values.
authors: Department of Molecular Biology and Chemistry, Analytical Chemistry, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
authors: E Carina
authors: EC
authors: Berglund
authors: Nicholas J
authors: NJ
authors: Kuklinski
authors: Ekin
authors: E
authors: Karagündüz
authors: Kubra
authors: K
authors: Ucar
authors: Jörg
authors: J
authors: Hanrieder
authors: Andrew G
authors: AG
authors: Ewing
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D001921:Brain / Q000166:cytology*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004331:Drosophila melanogaster; D004563:Electrochemistry; D005612:Freeze Drying / Q000379:methods*; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D001679:Biogenic Amines; D014867:Water
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