title: Development and validation of a rapid ultra high pressure liquid chromatographic method for the determination of methylxanthines in herbal infusions.
abstract: An ultra high pressure liquid chromatographic method coupled with diode array detector (UHPLC-DAD) has been developed and validated for the fast separation and determination of three major methylxanthines, i.e., caffeine, theophylline and theobromine, in various herbal beverages. Isocratic elution using 0.1vol% formic acid/CH3OH (92.5:7.5, v/v) enabled the completion of the separation cycle in less than 3min using a flow rate of 0.7mL/min and a column temperature of 50°C. Validation of the method included linearity (0.5-50mg/L), limits of detection (12-35μg/L) and quantification (40-120μg/L), precision, matrix effect and accuracy. The percent recoveries ranged between 90 and 108%.
authors: Research Laboratory for the Physical and Chemical Testing of Foods, Department of Food Technology, School of Food Technology and Nutrition, Alexander Technological Educational Institute (ATEI) of Thessaloniki, GR-57400, Thessaloniki, Greece. zacharis@chem.auth.gr
authors: Constantinos K
authors: CK
authors: Zacharis
authors: Fotini S
authors: FS
authors: Kika
authors: Paraskevas D
authors: PD
authors: Tzanavaras
authors: Konstantinos
authors: K
authors: Fytianos
mesh_terms: D001628:Beverages / Q000032:analysis*; D028241:Camellia sinensis / Q000737:chemistry; D020843:Chamomile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013662:Tea / Q000737:chemistry*; D014970:Xanthines / Q000032:analysis* / Q000737:chemistry
chemical_list: D013662:Tea; D014970:Xanthines