title: Postprandial changes in high density lipoproteins in rats subjected to gavage administration of virgin olive oil.
abstract: OBJECTIVE
  The present study was designed to verify the influence of acute fat loading on high density lipoprotein (HDL) composition, and the involvement of liver and different segments of small intestine in the changes observed.
  
  
  RESULTS
  To address these issues, rats were administered a bolus of 5-ml of extra-virgin olive oil and sacrificed 4 and 8 hours after feeding. In these animals, lipoproteins were analyzed and gene expressions of apolipoprotein and HDL enzymes were assessed in duodenum, jejunum, ileum and liver. Using this experimental design, total plasma and HDL phospholipids increased at the 8-hour-time-point due to increased sphingomyelin content. An increase in apolipoprotein A4 was also observed mainly in lipid-poor HDL. Increased expression of intestinal Apoa1, Apoa4 and Sgms1 mRNA was accompanied by hepatic decreases in the first two genes in liver. Hepatic expression of Abcg1, Apoa1bp, Apoa2, Apoe, Ptlp, Pon1 and Scarb1 decreased significantly following fat gavage, while no changes were observed for Abca1, Lcat or Pla2g7. Significant associations were also noted for hepatic expression of apolipoproteins and Pon1. Manipulation of postprandial triglycerides using an inhibitor of microsomal transfer protein -CP-346086- or of lipoprotein lipase -tyloxapol- did not influence hepatic expression of Apoa1 or Apoa4 mRNA.
  
  
  CONCLUSIONS
  All these data indicate that dietary fat modifies the phospholipid composition of rat HDL, suggesting a mechanism of down-regulation of hepatic HDL when intestine is the main source of those particles and a coordinated regulation of hepatic components of these lipoproteins at the mRNA level, independently of plasma postprandial triglycerides.
authors: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, Zaragoza, Spain.
authors: Roberto
authors: R
authors: Martínez-Beamonte
authors: María A
authors: MA
authors: Navarro
authors: Sergio
authors: S
authors: Acin
authors: Natalia
authors: N
authors: Guillén
authors: Cristina
authors: C
authors: Barranquero
authors: Carmen
authors: C
authors: Arnal
authors: Joaquín
authors: J
authors: Surra
authors: Jesus
authors: J
authors: Osada
mesh_terms: D000284:Administration, Oral; D000818:Animals; D020869:Gene Expression Profiling; D007421:Intestine, Small / Q000201:enzymology / Q000378:metabolism*; D007546:Isoquinolines; D008055:Lipids / Q000097:blood; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D011092:Polyethylene Glycols; D019518:Postprandial Period / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000097:blood; D051381:Rats; D013109:Sphingomyelins / Q000378:metabolism; D014230:Triazoles; D014280:Triglycerides / Q000378:metabolism
chemical_list: C493884:CP-346086; D007546:Isoquinolines; D008055:Lipids; D008075:Lipoproteins, HDL; D000069463:Olive Oil; D010938:Plant Oils; D012333:RNA, Messenger; D013109:Sphingomyelins; D014230:Triazoles; D014280:Triglycerides; D011092:Polyethylene Glycols; C016811:tyloxapol
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