title: MALDI-TOF mass spectrometry for the monitoring of she-donkey's milk contamination or adulteration.
abstract: Donkey's milk (DM), representing a safe and alternative food in both IgE-mediated and non-IgE-mediated cow's milk protein allergy, can be categorized as precious pharma-food. Moreover, an economically relevant interest for the use of DM in cosmetology is also developing. The detection of adulterations and contaminations of DM is a matter of fundamental importance from both an economic and allergenic standpoint, and, to this aim, fast and efficient analytical approaches to assess the authenticity of this precious nutrient are desirable. Here, a rapid matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS)-based method aimed to the detection of bovine or caprine milk in raw DM is reported. The presence of the extraneous milks was revealed by monitoring the protein profiles of the most abundant whey proteins, α-lactalbumin (α-LA) and β-lactoglobulin, used as molecular markers. The possibility of obtaining a quantitative analysis of the level of cow or goat milk in DM based on the MALDI-TOF peak areas of α-LAs was also explored. The results showed that the experimental quantitative values were in good agreement with the real composition of each mixture. As pretreatment of the milk samples is not required, and owing to the speed and the high sensitivity of MALDI-MS, the protocol here reported could represent a reliable method for routine analyses aimed to assess the absence of contamination in raw fresh DM samples.
authors: Department of Chemical Science, University of Catania, Viale A. Doria 6, 95125, Catania, Italy. vcunsolo@unict.it
authors: Vincenzo
authors: V
authors: Cunsolo
authors: Vera
authors: V
authors: Muccilli
authors: Rosaria
authors: R
authors: Saletti
authors: Salvatore
authors: S
authors: Foti
mesh_terms: D000818:Animals; D002417:Cattle; D018397:Equidae; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D016014:Linear Models; D008892:Milk / Q000737:chemistry* / Q000145:classification*; D008894:Milk Proteins / Q000032:analysis / Q000737:chemistry; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D008894:Milk Proteins