title: Similarities in the endocrine-disrupting potencies of indoor dust and flame retardants by using human osteosarcoma (U2OS) cell-based reporter gene assays.
abstract: Indoor dust is a sink for many kinds of pollutants, including flame retardants (FRs), plasticizers, and their contaminants and degradation products. These pollutants can be migrated to indoor dust from household items such as televisions and computers. To reveal high-priority end points of and contaminant candidates in indoor dust, using CALUX reporter gene assays based on human osteosarcoma (U2OS) cell lines, we evaluated and characterized the endocrine-disrupting potencies of crude extracts of indoor dust collected from Japan (n = 8), the United States (n = 21), Vietnam (n = 10), the Philippines (n = 17), and Indonesia (n = 10) and for 23 selected FRs. The CALUX reporter gene assays used were specific for compounds interacting with the human androgen receptor (AR), estrogen receptor α (ERα), progesterone receptor (PR), glucocorticoid receptor (GR), and peroxisome proliferator-activated receptor γ2 (PPARγ2). Indoor dust extracts were agonistic to ERα, GR, and PPARγ2 and antagonistic against AR, PR, GR, and PPARγ2. In comparison, a majority of FRs was agonistic to ERα and PPARγ2 only, and some FRs demonstrated receptor-specific antagonism against all tested nuclear receptors. Hierarchical clustering clearly indicated that agonism of ERα and antagonism of AR and PR were common, frequently detected end points for indoor dust and tested FRs. Given our previous results regarding the concentrations of FRs in indoor dust and in light of our current results, candidate contributors to these effects include not only internationally controlled brominated FRs but also alternatives such as some phosphorus-containing FRs. In the context of indoor pollution, high-frequency effects of FRs such as agonism of ERα and antagonism of AR and PR are candidate high-priority end points for further investigation.
authors: Center for Material Cycles and Waste Management Research, National Institute for Environmental Studies, Tsukuba, Japan, Tsukuba 305-8506, Japan. gsuzuki@nies.go.jp
authors: Go
authors: G
authors: Suzuki
authors: Nguyen Minh
authors: NM
authors: Tue
authors: Govindan
authors: G
authors: Malarvannan
authors: Agus
authors: A
authors: Sudaryanto
authors: Shin
authors: S
authors: Takahashi
authors: Shinsuke
authors: S
authors: Tanabe
authors: Shin-ichi
authors: S
authors: Sakai
authors: Abraham
authors: A
authors: Brouwer
authors: Naoto
authors: N
authors: Uramaru
authors: Shigeyuki
authors: S
authors: Kitamura
authors: Hidetaka
authors: H
authors: Takigami
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects*; D016902:Air Pollution, Indoor / Q000009:adverse effects*; D001681:Biological Assay; D045744:Cell Line, Tumor; D004391:Dust / Q000032:analysis; D052244:Endocrine Disruptors / Q000009:adverse effects*; D005411:Flame Retardants / Q000009:adverse effects*; D017930:Genes, Reporter; D006801:Humans
chemical_list: D000393:Air Pollutants; D004391:Dust; D052244:Endocrine Disruptors; D005411:Flame Retardants