title: microRNA-16 represses colorectal cancer cell growth in vitro by regulating             the p53/survivin signaling pathway.
abstract: Dysregulated expression of microRNAs (miRNA) is a hallmark of cancer. miR-16             has been reported to be downregulated and to act as a tumor suppressor in different             cancer types. In the present study, we sought to investigate the possible roles             and mechanisms of miR-16 and its relationship with p53 and survivin in CRC cells.             We showed that miR-16 was downregulated in 67% of CRC tissues and was correlated             with the degree of histological differentiation. Experiments in vitro showed that             overexpression of miR-16 inhibited the proliferation and induced apoptosis of             CRC cells through the intrinsic apoptosis pathway. We further showed that miR-16             repressed survivin expression at both the mRNA and protein levels and the survivin             gene was a direct target of miR-16. In addition, miR-16 reduced p53 expression             and p53 increased miR-16 levels, with downregulation of miR-16 targets survivin,             cyclin D1 and CDK6. Our findings suggest that miR-16 represses colorectal cancer             cell growth in vitro by regulating the p53/survivin signaling pathway. Our findings             provide further evidence for the involvement of dysregulated miRNAs in CRC, and             miR-16 could serve as a molecular target for CRC therapy.
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, PR China.
authors: Qunying
authors: Q
authors: Ma
authors: Xinying
authors: X
authors: Wang
authors: Zhao
authors: Z
authors: Li
authors: Bingsheng
authors: B
authors: Li
authors: Fengli
authors: F
authors: Ma
authors: Liang
authors: L
authors: Peng
authors: Yu
authors: Y
authors: Zhang
authors: Angao
authors: A
authors: Xu
authors: Bo
authors: B
authors: Jiang
mesh_terms: D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D058990:Molecular Targeted Therapy; D009367:Neoplasm Staging; D015398:Signal Transduction / Q000235:genetics; D000077022:Survivin; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C505362:MIRN16 microRNA, human; D035683:MicroRNAs; D000077022:Survivin; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53