title: Evaluation of mutagenicity of wastewater in the vicinity of pesticide industry.
abstract: Pesticide industrial wastewater samples were taken from the Chinhat industrial area nearby Lucknow city, India. GC-MS analysis revealed the presence of pesticides lindane, α-endosulfan, β-endosulfan, chlorpyriphos, monocrotophos, dimethoate and malathion. A pesticide mixture and wastewater extracts were studied to determine the mutagenicity by Ames Salmonella test, survival of DNA repair defective E. coli K-12 mutants and bacteriophage λ systems. Wastewater samples were concentrated with XAD-resins as an adsorbent and liquid-liquid extraction procedure. The XAD concentrated sample exhibited maximum mutagenic activity in comparison to liquid-liquid extracted sample. TA98 strain was the most responsive strain for both test samples with (+S9) and without (-S9) metabolic activation, while other strains exhibited weak response. A significant decline of DNA repair defective E. coli K-12 mutants, bacteriophage λ was observed with test samples in the survival. The intracellular damage was highest when treated with XAD concentrated sample as compared to liquid-liquid extract after 6h treatment.
authors: Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202 002 (UP), India. reshmabiotec@gmail.com
authors: Reshma
authors: R
authors: Anjum
authors: Abdul
authors: A
authors: Malik
mesh_terms: D010582:Bacteriophage lambda / Q000187:drug effects; D002617:Chemical Industry; D004260:DNA Repair / Q000187:drug effects; D004726:Endosulfan / Q000032:analysis*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D048168:Escherichia coli K12 / Q000187:drug effects / Q000235:genetics; D007194:India; D007220:Industrial Waste / Q000032:analysis; D009152:Mutagenicity Tests / Q000379:methods*; D010575:Pesticides / Q000032:analysis*; D012475:Salmonella / Q000187:drug effects / Q000235:genetics; D062065:Wastewater / Q000032:analysis* / Q000633:toxicity*
chemical_list: D004726:Endosulfan; D007220:Industrial Waste; D010575:Pesticides; D062065:Wastewater