title: Overexpression of MyrAkt1 in endothelial cells leads to erythropoietin- and BMP4-independent splenic erythropoiesis in mice.
abstract: Under steady state conditions, erythropoiesis occurs in the bone marrow. However, in mice, stress or tissue hypoxia results in increased erythropoiesis in the spleen. There is increasing evidence that the hematopoietic microenvironment, including endothelial cells, plays an important role in regulating erythropoiesis. Here, we show that short-term expression of constitutively active Akt in the endothelium of mice results in non-anemic stress erythropoiesis in the spleen. The initiation of this stress response was independent of erythropoietin and BMP4, and was observed in endothelial myrAkt1 mice reconstituted with wild-type bone marrow. Together, these data suggest that endothelial cell hyperactivation is a potentially novel pathway of inducing red cell production under stress.
authors: Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA. rebekah.k.odonnell@gmail.com
authors: Rebekah K
authors: RK
authors: O'Donnell
authors: Whitney E
authors: WE
authors: Goldstein
authors: Carole
authors: C
authors: Perruzzi
authors: Laura E
authors: LE
authors: Benjamin
authors: William C
authors: WC
authors: Aird
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D004920:Erythropoiesis / Q000502:physiology; D004921:Erythropoietin / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D011247:Pregnancy; D046188:Protein Modification, Translational; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics* / Q000378:metabolism*; D013154:Spleen / Q000166:cytology* / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D055415:Bone Morphogenetic Protein 4; D004921:Erythropoietin; D051057:Proto-Oncogene Proteins c-akt
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