title: Near-infrared fluorescence imaging for noninvasive trafficking of scaffold degradation.
abstract: Biodegradable scaffolds could revolutionize tissue engineering and regenerative medicine; however, in vivo matrix degradation and tissue ingrowth processes are not fully understood. Currently a large number of samples and animals are required to track biodegradation of implanted scaffolds, and such nonconsecutive single-time-point information from various batches result in inaccurate conclusions. To overcome this limitation, we developed functional biodegradable scaffolds by employing invisible near-infrared fluorescence and followed their degradation behaviors in vitro and in vivo. Using optical fluorescence imaging, the degradation could be quantified in real-time, while tissue ingrowth was tracked by measuring vascularization using magnetic resonance imaging in the same animal over a month. Moreover, we optimized the in vitro process of enzyme-based biodegradation to predict implanted scaffold behaviors in vivo, which was closely related to the site of inoculation. This combined multimodal imaging will benefit tissue engineers by saving time, reducing animal numbers, and offering more accurate conclusions.
authors: Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Soon Hee
authors: SH
authors: Kim
authors: Jeong Heon
authors: JH
authors: Lee
authors: Hoon
authors: H
authors: Hyun
authors: Yoshitomo
authors: Y
authors: Ashitate
authors: Gwangli
authors: G
authors: Park
authors: Kyle
authors: K
authors: Robichaud
authors: Elaine
authors: E
authors: Lunsford
authors: Sang Jin
authors: SJ
authors: Lee
authors: Gilson
authors: G
authors: Khang
authors: Hak Soo
authors: HS
authors: Choi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism; D007259:Infrared Rays; D008279:Magnetic Resonance Imaging; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019265:Spectroscopy, Near-Infrared; D013451:Sulfonic Acids / Q000737:chemistry; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; C562069:ZW800-1 compound; D003094:Collagen; D017364:Collagenases
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