title: Cytokine profile in human eyes: contribution of a new cytokine combination for differential diagnosis between intraocular lymphoma or uveitis.
abstract: Primary intraocular lymphoma (PIOL), also called primary vitreoretinal lymphomas, often masquerades as uveitis. This misdiagnosis can result in subsequent brain involvement and oculocerebral lymphoma (OCL). In this study, we sought to characterize the helper T-cell type 1 (Th1)/Th2 cytokine profile in vitreous samples from patients with PIOL, OCL, uveitis and controls with non-inflammatory disease. Vitreous and aqueous humor samples from 87 patients with PIOL (n = 30), OCL (n = 12), uveitis (n = 34), and retinal detachment (RD) without hemorrhage (n = 11) were analyzed and their concentrations of interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were determined by flow cytometric bead arrays (CBA). The IL-10 levels determined by CBA were compared with those by ELISA. IL-10 concentrations measured by CBA and ELISA were highly correlated. IL-2, IL-4, and TNFα were not detected in any sample. The only cytokine detected at a significant level in samples from RD vitreous was IL-6. The IL-10/IL-6 ratio, as previously reported, was slightly higher in PIOL than in uveitis samples, but not for all patients. Cytokine profiles from PIOL and OCL samples did not differ. The combination of the IL-10/IL-6 and IL-10/IFNγ ratios was highly informative for discriminating PIOL/OCL from uveitis samples and for therapeutic follow up of PIOL. This strategy might be very helpful as an initial screening to rule out PIOL in patients thought to have uveitis.
authors: Institut National de la Santé et de la Recherche Médicale, UMRS872, Centre de Recherche des Cordeliers, Paris, France. sylvain.fisson@univ-evry.fr
authors: Sylvain
authors: S
authors: Fisson
authors: Hanane
authors: H
authors: Ouakrim
authors: Valérie
authors: V
authors: Touitou
authors: Sylvie
authors: S
authors: Baudet
authors: Rym
authors: R
authors: Ben Abdelwahed
authors: Sabrina
authors: S
authors: Donnou
authors: Amine
authors: A
authors: Miloudi
authors: Claire
authors: C
authors: Galand
authors: Bahram
authors: B
authors: Bodaghi
authors: Phuc
authors: P
authors: Lehoang
authors: Martine
authors: M
authors: Brissard
authors: Magali
authors: M
authors: Le Garff-Tavernier
authors: Wolf Herman
authors: WH
authors: Fridman
authors: Catherine
authors: C
authors: Sautès-Fridman
authors: Nathalie
authors: N
authors: Cassoux
authors: Hélène
authors: H
authors: Merle-Béral
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000276:immunology / Q000378:metabolism; D002648:Child; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D003937:Diagnosis, Differential; D005134:Eye Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000276:immunology / Q000378:metabolism; D008223:Lymphoma / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism; D014605:Uveitis / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016207:Cytokines; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
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