title: MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in the cells. However, certain miRNA* express abundantly and can be recruited into the silencing complex to regulate gene expression. Most miRNAs function as suppressive regulators on gene expression. Group B coxsackieviruses (CVB) are the major pathogens of human viral myocarditis and dilated cardiomyopathy. CVB genome is a positive-sense, single-stranded RNA. Our previous study shows that miR-342-5p can suppress CVB biogenesis by targeting its 2C-coding sequence. In this study, we found that the miR-10a duplex could significantly up-regulate the biosynthesis of CVB type 3 (CVB3). Further study showed that it was the miR-10a star strand (miR-10a*) that augmented CVB3 biosynthesis. Site-directed mutagenesis showed that the miR-10a* target was located in the nt6818-nt6941 sequence of the viral 3D-coding region. MiR-10a* was detectable in the cardiac tissues of suckling Balb/c mice, suggesting that miR-10a* may impact CVB3 replication during its cardiac infection. Taken together, these data for the first time show that miRNA* can positively modulate gene expression. MiR-10a* might be involved in the CVB3 cardiac pathogenesis.
authors: Department of Microbiology, Harbin Medical University, 157 Baojian Road, Harbin 150081, China.
authors: Lei
authors: L
authors: Tong
authors: Lexun
authors: L
authors: Lin
authors: Shuo
authors: S
authors: Wu
authors: Zhiwei
authors: Z
authors: Guo
authors: Tianying
authors: T
authors: Wang
authors: Ying
authors: Y
authors: Qin
authors: Ruixue
authors: R
authors: Wang
authors: Xiaoyan
authors: X
authors: Zhong
authors: Xia
authors: X
authors: Wu
authors: Yan
authors: Y
authors: Wang
authors: Tian
authors: T
authors: Luan
authors: Qiang
authors: Q
authors: Wang
authors: Yunxia
authors: Y
authors: Li
authors: Xiaofeng
authors: X
authors: Chen
authors: Fengmin
authors: F
authors: Zhang
authors: Wenran
authors: W
authors: Zhao
authors: Zhaohua
authors: Z
authors: Zhong
mesh_terms: D000818:Animals; D029822:Enterovirus B, Human / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D049410:Luciferases, Renilla / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: C547183:MIRN10 microRNA, human; C569259:MIRN10 microRNA, mouse; D035683:MicroRNAs; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D049410:Luciferases, Renilla
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