title: Development of a bioluminescent bacteria sheet for the measurement of oxygen concentration.
abstract: The aim of this study was the preparation and characterization of a bioluminescence bacteria sheet in view of the fabrication of an oxygen marker inside a biofilm.  Photobacterium kishitanii sheets were prepared using sodium alginate, and a biofilm of Pseudomonas aeruginosa was dropped over it.  The relationship between the dissolved oxygen (DO) concentration in the biofilm and luminescence from the sheet was examined.  As a conclusion, the alginate-gel film method was effective in fabricating a DO-monitoring thin film.
authors: School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo, Japan. sasaki@stf.teu.ac.jp
authors: Satoshi
authors: S
authors: Sasaki
authors: Mika
authors: M
authors: Mochizuki
authors: Makoto
authors: M
authors: Igarashi
mesh_terms: D000464:Alginates / Q000737:chemistry; D018441:Biofilms / Q000254:growth & development; D015374:Biosensing Techniques / Q000379:methods*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D010776:Photobacterium / Q000166:cytology* / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000502:physiology
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D010100:Oxygen