title: Survival of hypoxic human mesenchymal stem cells is enhanced by a positive feedback loop involving miR-210 and hypoxia-inducible factor 1.
abstract: The use of mesenchymal stem cells (MSCs) has emerged as a potential new treatment for myocardial infarction. However, the poor viability of MSCs after transplantation critically limits the efficacy of this new strategy. The expression of microRNA-210 (miR-210) is induced by hypoxia and is important for cell survival under hypoxic conditions. Hypoxia increases the levels of hypoxia inducible factor-1 (HIF-1) protein and miR-210 in human MSCs (hMSCs). miR-210 positively regulates HIF-1α activity. Furthermore, miR-210 expression is also induced by hypoxia through the regulation of HIF-1α. To investigate the effect of miR-210 on hMSC survival under hypoxic conditions, survival rates along with signaling related to cell survival were evaluated in hMSCs over-expressing miR-210 or ones that lacked HIF-1α expression. Elevated miR-210 expression increased survival rates along with Akt and ERK activity in hMSCs with hypoxia. These data demonstrated that a positive feedback loop involving miR-210 and HIF-1α was important for MSC survival under hypoxic conditions.
authors: Department of Biology Education, College of Education, Pusan National University, Busan 609-735, Korea.
authors: Woochul
authors: W
authors: Chang
authors: Chang Youn
authors: CY
authors: Lee
authors: Jun-Hee
authors: JH
authors: Park
authors: Moon-Seo
authors: MS
authors: Park
authors: Lee-So
authors: LS
authors: Maeng
authors: Chee Soon
authors: CS
authors: Yoon
authors: Min Young
authors: MY
authors: Lee
authors: Ki-Chul
authors: KC
authors: Hwang
authors: Yong-An
authors: YA
authors: Chung
mesh_terms: D002470:Cell Survival; D003035:Cobalt; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D035683:MicroRNAs / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010101:Oxygen Consumption; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C543800:MIRN210 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D003035:Cobalt; C018021:cobaltous chloride; D010100:Oxygen
keywords: cell survival; human mesenchymal stem cells; hypoxia; hypoxia inducible factor-1; microRNA-210
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