title: [The mechanism of alteronol inhibiting the proliferation of human promyelocytic leukemia HL-60 cells].
abstract: This study is to investigate the mechanism of human promyelocytic leukemia HL-60 cells proliferation induced by alteronol in vitro. Human promyelocytic leukemia HL-60 cells cultured in vitro were treated with different concentrations of alteronol. Inhibition rate was detected by SRB assay. Cellular morphological changes were observed by Hoechst and AO/EB (acridine orange/ethidium bromide dye) staining. The apoptosis rate was determined by Annexin V-FITC/PI assay. Cell cycle distribution was determined by flow cytometry. Western blotting analysis was carried out to determine the cell cycle related proteins. The proliferation of HL-60 cells treated with alteronol was inhibited in a concentration-dependent manner. Based on cell viability assay, observation on cell morphology and apoptosis rate, it confirmed that alteronol played an obvious role in proliferation inhibition of human promyelocytic leukemia HL-60 cells, but it did not induce apoptosis in human promyelocytic leukemia HL-60 cells in different concentrations groups. Alteronol could effectively inhibit the proliferation of human promyelocytic leukemia HL-60 cells inducing cell cycle arrest at G1 phase, as well as, alteration expression of cell cycle proteins level of CyclinD1 and pRb.
authors: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, School ofPharmacy, Shihezi University, Shihezi 832002, China.
authors: Liang-Liang
authors: LL
authors: Liu
authors: Na
authors: N
authors: Chen
authors: Xuan
authors: X
authors: Yuan
authors: Ying
authors: Y
authors: Yao
authors: Bo
authors: B
authors: Zhang
authors: Qiu-Sheng
authors: QS
authors: Zheng
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D009285:Naphthoquinones / Q000008:administration & dosage / Q000494:pharmacology*; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D009285:Naphthoquinones; D016160:Retinoblastoma Protein; C571346:alteronol; D019938:Cyclin D1