title: Plant secretome proteomics.
abstract: The plant secretome refers to the set of proteins secreted out of the plant cell into the surrounding extracellular space commonly referred to as the apoplast. Secreted proteins maintain cell structure and acts in signaling and are crucial for stress responses where they can interact with pathogen effectors and control the extracellular environment. Typically, secreted proteins contain an N-terminal signal peptide and are directed through the endoplasmic reticulum/Golgi pathway. However, in plants many proteins found in the secretome lack such a signature and might follow alternative ways of secretion. This review covers techniques to isolate plant secretomes and how to identify and quantify their constituent proteins. Furthermore, bioinformatical tools to predict secretion signals and define the putative secretome are presented. Findings from proteomic studies and important protein families of plant secretomes, such as proteases and hydrolases, are highlighted.
authors: Department of Plant Protection Biology, Swedish University of Agricultural Sciences Alnarp, Sweden.
authors: Erik
authors: E
authors: Alexandersson
authors: Ashfaq
authors: A
authors: Ali
authors: Svante
authors: S
authors: Resjö
authors: Erik
authors: E
authors: Andreasson
keywords: apoplast; mass spectrometry; plant; proteomics; secretome
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