title: Neurochemical profile of patients with type 1 diabetes measured by ¹H-MRS at 4 T.
abstract: The impact of type 1 diabetes mellitus (T1DM) on a comprehensive neurochemical profile of the human brain has not been reported yet. Our previous proton magnetic resonance spectroscopy ((1)H-MRS) studies on T1DM were focused exclusively on the assessment of brain glucose levels. In this study, we reexamined our previously acquired data to investigate concentration differences of a broad range of neurochemicals in T1DM subjects relative to nondiabetic controls. We selected MRS data from 13 subjects (4 F/9 M, age = 41 ± 11 years, body mass index = 26 ± 3 kg/m(2)) with well-controlled T1DM (disease duration = 22 ± 12 years, A1C = 7.5% ± 2.0%) and 32 nondiabetic controls (14 F/18 M, age = 36 ± 10 years, body mass index = 27 ± 6 kg/m(2)) acquired during a hyperglycemic clamp (target [Glc]plasma = 300 ± 15 mg/dL). The (1)H-MR spectra were collected from two 15.6-mL voxels localized in gray-matter-rich occipital lobe and in white-matter-rich parieto-occipital region using ultra-short echo-time STEAM at 4 T. LCModel analysis allowed reliable quantification of 17 brain metabolites. Lower levels of N-acetylaspartate (by 6%, P=0.007) and glutamate (by 6%, P=0.045) were observed in the gray matter of T1DM patients as compared with controls, which might indicate a partial neuronal loss or dysfunction as a consequence of long-term T1DM. No other differences in metabolites were observed between subjects with T1DM and controls.
authors: Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, Minnesota 55455, USA. mangia@umn.edu
authors: Silvia
authors: S
authors: Mangia
authors: Anjali F
authors: AF
authors: Kumar
authors: Amir A
authors: AA
authors: Moheet
authors: Rachel J
authors: RJ
authors: Roberts
authors: Lynn E
authors: LE
authors: Eberly
authors: Elizabeth R
authors: ER
authors: Seaquist
authors: Ivan
authors: I
authors: Tkáč
mesh_terms: D000328:Adult; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D018698:Glutamic Acid / Q000032:analysis / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D018377:Neurotransmitter Agents / Q000032:analysis / Q000378:metabolism; D009778:Occipital Lobe / Q000378:metabolism / Q000473:pathology
chemical_list: D018377:Neurotransmitter Agents; D001224:Aspartic Acid; D018698:Glutamic Acid; C000179:N-acetylaspartate
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