title: Significance of antibodies against the native ribosomal P protein complex and recombinant P0, P1, and P2 proteins in the diagnosis of Chinese patients with systemic lupus erythematosus.
abstract: BACKGROUND
  This study aimed to evaluate autoantibodies against the native ribosomal P complex (anti-Rib-P(C)) and recombinant ribosomal P proteins (anti-Rib-P0, anti-Rib-P1, anti-Rib-P2) for their prevalence, diagnostic relevance and clinical associations in a Chinese cohort with systemic lupus erythematosus (SLE).
  
  
  METHODS
  Anti-Rib-P, anti-dsDNA and anti-Smith antigen (Sm) antibodies were analyzed in sera from 198 patients with SLE, 33 with rheumatoid arthritis, 61 with Sjögren's syndrome and 70 healthy individuals by means of ELISA.
  
  
  RESULTS
  Antibody prevalences were 29.8% (anti-Rib-P(C)), 33.3% (anti-Rib-P0), 42.9% (anti-Rib-P1) and 34.3% (anti-Rib-P2), at a specificity of 99%. Among SLE patients lacking anti-dsDNA and anti-Sm, 27.8% showed positive for at least one of the investigated anti-Rib-P types. The serological hit rate provided by anti-dsDNA/anti-Sm detection (72.7%) was increased upon parallel testing for anti-Rib-P(C) (77.3%) or anti-Rib-P0/P1/P2 (80.3%). Anti-Rib-P positivity was associated with disease activity, neuropsychiatric events, lupus nephritis, skin rash, lymphocytopenia, increased erythrocyte sedimentation rates, decreased complement C3/C4 and elevated IgA/IgG levels.
  
  
  CONCLUSIONS
  Based on these results, antibodies against ribosomal P proteins are important complementary parameters to anti-dsDNA and anti-Sm, and should be considered for inclusion in the classification criteria for SLE. The diagnostic value of anti-Rib-P0/P1/P2 is diagnostically superior to that of anti-Rib-P(C).
authors: Department of Rheumatology and Immunology, People's Hospital, Peking University Medical School, Beijing, China.
authors: Jing
authors: J
authors: Li
authors: Ying
authors: Y
authors: Shen
authors: Jing
authors: J
authors: He
authors: Rulin
authors: R
authors: Jia
authors: Xiujie
authors: X
authors: Wang
authors: Xiaosan
authors: X
authors: Chen
authors: Dahai
authors: D
authors: Wang
authors: Lei
authors: L
authors: Han
authors: Lei
authors: L
authors: Zhu
authors: Xiaofeng
authors: X
authors: Chi
authors: Sandra
authors: S
authors: Saschenbrecker
authors: Cornelia
authors: C
authors: Dähnrich
authors: Winfried
authors: W
authors: Stöcker
authors: Wolfgang
authors: W
authors: Schlumberger
authors: Zhan-Guo
authors: ZG
authors: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012269:Ribosomal Proteins / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D001323:Autoantibodies; D010750:Phosphoproteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins
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