title: Contribution of CE to the analysis of protein or peptide biomarkers.
abstract: Biomarker analysis is pivotal for disease diagnosis and one important class of biomarkers is constituted by proteins and peptides. This review focuses on protein and peptide analyses from biological fluids performed by capillary electrophoresis. The various strategies that have been reported to prevent difficulties due to the handling of real samples are described. Innovative techniques to overcome the complexity of the sample, to prevent the adsorption of the analytes on the inner capillary wall, and to increase the sensibility of the analysis are summarized and illustrated by different applications. To fully illustrate the contribution of CE to the analysis of biomarkers from human sample, two detailed protocols are given: the analysis from CSF of five amyloid peptide, biomarkers of the Alzheimer disease, and the analysis of sialoforms of transferrin from human serum.
authors: Laboratory of Proteins and Nanotechnologies in Separation Sciences, Faculté de Pharmacie, University of Paris-Sud, UMR-CNRS 8612, Châtenay-Malabry, France.
authors: Kiarach
authors: K
authors: Mesbah
authors: Romain
authors: R
authors: Verpillot
authors: François
authors: F
authors: de L'escaille
authors: Jean Bernard
authors: JB
authors: Falmagne
authors: Myriam
authors: M
authors: Taverna
mesh_terms: D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D016564:Amyloid beta-Protein Precursor / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D002846:Chromatography, Affinity; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D010455:Peptides / Q000097:blood / Q000134:cerebrospinal fluid / Q000302:isolation & purification; D014168:Transferrin / Q000302:isolation & purification / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; D010455:Peptides; D014168:Transferrin; D000509:alpha-Fetoproteins