title: Quantitative analysis of pancreatic polypeptide cell distribution in the human pancreas.
abstract: The pancreatic islet is mainly composed of beta-, alpha- and delta-cells with small numbers of pancreatic polypeptide (PP) and epsilon cells. It is known that there is a region in the head of the pancreas that is rich in PP-cells. In the present study, we examined the distribution of PP-cells, and assessed the influence of the PP-cell rich region to quantify the total islet mass. Pancreatic tissues were collected from donors with no history of diabetes or pancreatic diseases (n = 12). A stereological approach with a computer-assisted large-scale analysis of whole pancreatic sections was applied to quantify the entire distribution of endocrine cells within a given section. The initial whole pancreas analysis showed that a PP-cell rich region was largely restricted to the uncinate process with a clear boundary. The distinct distribution of PP-cells includes irregularly shaped clusters composed solely of PP-cells. Furthermore, in the PP-cell rich region, beta- and alpha-cell mass is significantly reduced compared to surrounding PP-cell poor regions. The results suggest that the analysis of the head region should distinguish the PP-cell rich region, which is best examined separately. This study presents an important implication for the regional selection and interpretation of the results.
authors: Department of Surgery, The University of Chicago, Chicago, Illinois, United States of America.
authors: Xiaojun
authors: X
authors: Wang
authors: Mark C
authors: MC
authors: Zielinski
authors: Ryosuke
authors: R
authors: Misawa
authors: Patrick
authors: P
authors: Wen
authors: Tian-Yuan
authors: TY
authors: Wang
authors: Cheng-Zhang
authors: CZ
authors: Wang
authors: Piotr
authors: P
authors: Witkowski
authors: Manami
authors: M
authors: Hara
mesh_terms: D050416:Glucagon-Secreting Cells / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D050417:Insulin-Secreting Cells / Q000166:cytology; D007515:Islets of Langerhans; D010179:Pancreas / Q000033:anatomy & histology / Q000166:cytology* / Q000378:metabolism; D050418:Pancreatic Polypeptide-Secreting Cells / Q000166:cytology* / Q000378:metabolism
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