title: Impact of the next-generation sequencing data depth on various biological result inferences.
abstract: Next-generation sequencing (NGS) technologies have revolutionized the field of genomics and provided unprecedented opportunities for high-throughput analysis at the levels of genomics, transcriptomics and epigenetics. However, the cost of NGS is still prohibitive for many laboratories. It is imperative to address the trade-off between the sequencing depth and cost. In this review, we will discuss the effects of sequencing depth on the detection of genes, quantification of gene expression and discovering of gene structural variants. This will provide readers information on choosing appropriate sequencing depth that best meet the needs of their particular project.
authors: National Engineering Center for Biochip at Shanghai, Shanghai 201203, China.
authors: Rui
authors: R
authors: Hou
authors: Zhenxing
authors: Z
authors: Yang
authors: Minghui
authors: M
authors: Li
authors: Huasheng
authors: H
authors: Xiao
mesh_terms: D003365:Costs and Cost Analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D014644:Genetic Variation; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics / Q000706:statistics & numerical data*; D006801:Humans; D017423:Sequence Analysis, RNA / Q000191:economics / Q000706:statistics & numerical data