title: Production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from Lysinibacillus fusiformis.
abstract: A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction conditions for the production of 10,12-dihydroxystearic acid were optimized as follows: pH 6.5, 30 °C, 15 g l(-1) ricinoleic acid, 9 mg ml(-1) of enzyme, and 4 % (v/v) methanol. Under the optimized conditions, the enzyme produced 13.5 g l(-1) 10,12-dihydroxystearic acid without detectable byproducts in 3 h, with a conversion of substrate to product of 90 % (w/w) and a productivity of 4.5 g l(-1) h(-1). The emulsifying activity of 10,12-dihydroxystearic acid was higher than that of oleic acid, ricinoleic acid, stearic acid, and 10-hydroxystearic acid, indicating that 10,12-dihydroxystearic acid can be used as a biosurfactant.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Min-Ho
authors: MH
authors: Seo
authors: Kyoung-Rok
authors: KR
authors: Kim
authors: Deok-Kun
authors: DK
authors: Oh
mesh_terms: D001406:Bacillaceae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D015394:Molecular Structure; D019301:Oleic Acid / Q000378:metabolism*; D012277:Ricinoleic Acids / Q000378:metabolism*; D013229:Stearic Acids / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012277:Ricinoleic Acids; D013229:Stearic Acids; D019301:Oleic Acid; C030521:ricinoleic acid