title: Optimization of Douchi fibrinolytic enzyme production by statistical experimental methods.
abstract: Thrombus disease, one of the common cardiovascular diseases, has attracted worldwide attention for its rising mortality and morbidity. Due to the distinct shortages of current fibrinolytic drugs, new fibrinolytic agents warrant investigation. In this study, 8 fibrinolytic enzyme-producing strains were isolated from Douchi-a traditional Chinese food, and strain XY-1 which produced the largest amount of the enzyme was chosen for the following experiments. The enzyme produced by strain XY-1 was named Douchi fibrinolytic enzyme (DFE). We optimized the liquid culture medium of strain XY-1 for enzyme production using Plackett-Burman and Box-Behnken design. The predicted maximal DFE yield was 19.78 FU/mL with 11.4 g/L peptone, 0.5 g/L magnesium sulfate and 1 g/L sodium chloride. However, we acquired maximal production of 21.33 FU/mL in actual experiments, equal to 107.84% of the theoretical value, and the yield had been increased by 79.55% as compared to the yield of un-optimized culture. It was demonstrated that the combined use of Plackett-Burman design and response surface methodology in fermentation optimization can effectively and rapidly increase DFE production.
authors: MOE Key Lab of Environment and Health, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Xu
authors: X
authors: Zhang
authors: MOE Key Lab of Environment and Health, Huazhong University of Science and Technology, Wuhan, 430030, China. yunluojia03@163.com.
authors: Luo-Jia
authors: LJ
authors: Yun
authors: Department of Epidemiology and Health Statistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Liang-Bin
authors: LB
authors: Peng
authors: MOE Key Lab of Environment and Health, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Yi
authors: Y
authors: Lu
authors: MOE Key Lab of Environment and Health, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Kun-Peng
authors: KP
authors: Ma
authors: MOE Key Lab of Environment and Health, Huazhong University of Science and Technology, Wuhan, 430030, China. feitang@mails.tjmu.edu.cn.
authors: Fei
authors: F
authors: Tang
mesh_terms: D001407:Bacillus / Q000502:physiology*; D019149:Bioreactors / Q000382:microbiology; D001777:Blood Coagulation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D020650:Combinatorial Chemistry Techniques; D003198:Computer Simulation; D007887:Fabaceae / Q000201:enzymology* / Q000254:growth & development / Q000382:microbiology; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D008954:Models, Biological; D015233:Models, Statistical
chemical_list: D005343:Fibrinolytic Agents
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