title: Protective effect of cepharanthin on cisplatin-induced renal toxicity through metallothionein expression.
abstract: OBJECTIVE
  Cisplatin (CDDP) is a potent anticancer agent, but severe renal toxicity can limit its use. We investigated the protective effect of cepharanthin (CE), a biscoclaurin alkaloid, on the renal toxicity of CDDP.
  
  
  METHODS
  Mice were given CDDP along with CE. Effects of CE on CDDP toxicity were investigated by assaying markers of renal toxicity together with MT expression, and by histopathological examination of the kidney. MT-null mice were also examined.
  
  
  RESULTS
  CE induced expression of metallothionein (MT). Pre-administration of CE attenuated an increase in blood urea nitrogen (BUN) concentrations after the CDDP injection. A histochemical analysis demonstrated protection against CDDP-induced necrocytosis of kidney tissues by CE. The protective effect of CE did not occur in the MT-null mice.
  
  
  CONCLUSIONS
  Pretreatment with CE may reduce the renal toxicity of CDDP through expression of MT.
authors: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan. sogawa@md.okayama-u.ac.jp
authors: Norio
authors: N
authors: Sogawa
authors: Kanji
authors: K
authors: Hirai
authors: Chiharu
authors: C
authors: Sogawa
authors: Kazumi
authors: K
authors: Ohyama
authors: Ikuko
authors: I
authors: Miyazaki
authors: Goichi
authors: G
authors: Tsukamoto
authors: Masato
authors: M
authors: Asanuma
authors: Akira
authors: A
authors: Sasaki
authors: Shigeo
authors: S
authors: Kitayama
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D044182:Benzylisoquinolines / Q000627:therapeutic use*; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D002945:Cisplatin / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000235:genetics / Q000517:prevention & control*; D008297:Male; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects; D052117:Benzodioxoles
chemical_list: D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D044182:Benzylisoquinolines; D002945:Cisplatin; D008668:Metallothionein; D012333:RNA, Messenger; C006947:cepharanthine; D052117:Benzodioxoles