title: Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays.
abstract: We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole-imidazole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. These polyamide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonucleotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch.
authors: Department of Pure & Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, United Kingdom.
authors: Ishwar
authors: I
authors: Singh
authors: Christian
authors: C
authors: Wendeln
authors: Alasdair W
authors: AW
authors: Clark
authors: Jonathan M
authors: JM
authors: Cooper
authors: Bart Jan
authors: BJ
authors: Ravoo
authors: Glenn A
authors: GA
authors: Burley
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D057930:Click Chemistry; D004247:DNA / Q000032:analysis*; D007093:Imidazoles / Q000737:chemistry*; D015394:Molecular Structure; D009757:Nylons / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles; C029899:imidazole; D004247:DNA