title: Virus activated artificial ECM induces the osteoblastic differentiation of mesenchymal stem cells without osteogenic supplements.
abstract: Biochemical and topographical features of an artificial extracellular matrix (aECM) can direct stem cell fate. However, it is difficult to vary only the biochemical cues without changing nanotopography to study their unique role. We took advantage of two unique features of M13 phage, a non-toxic nanofiber-like virus, to generate a virus-activated aECM with constant ordered ridge/groove nanotopography but displaying different fibronectin-derived peptides (RGD, its synergy site PHSRN, and a combination of RGD and PHSRN). One feature is the self-assembly of phage into a ridge/groove structure, another is the ease of genetically surface-displaying a peptide. We found that the unique ridge/groove nanotopography and the display of RGD and PHSRN could induce the osteoblastic differentiation of mesenchymal stem cells (MSCs) without any osteogenic supplements. The aECM formed through self-assembly and genetic engineering of phage can be used to understand the role of peptide cues in directing stem cell behavior while keeping nanotopography constant.
authors: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, OK 73019, USA.
authors: Jianglin
authors: J
authors: Wang
authors: Lin
authors: L
authors: Wang
authors: Xin
authors: X
authors: Li
authors: Chuanbin
authors: C
authors: Mao
mesh_terms: D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics; D018441:Biofilms; D002448:Cell Adhesion; D002454:Cell Differentiation; D005109:Extracellular Matrix / Q000821:virology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D057139:Nanofibers; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology*; D019151:Peptide Library; D051381:Rats
chemical_list: D009842:Oligopeptides; D019151:Peptide Library; C047981:arginyl-glycyl-aspartic acid
references: Cell. 2006 Aug 25;126(4):645-7
references: 16923382
references: Cell. 2008 Feb 22;132(4):598-611
references: 18295578
references: Tissue Eng. 2001 Feb;7(1):89-99
references: 11224927
references: Nat Immunol. 2005 Mar;6(3):314-22
references: 15665828
references: Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2130-5
references: 19179282
references: J Biomed Mater Res A. 2009 Oct;91(1):195-208
references: 18814275
references: Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16489-94
references: 15520394
references: Angew Chem Int Ed Engl. 2010;49(5):868-72
references: 20013831
references: J Dent Res. 2005 May;84(5):407-13
references: 15840774
references: Biomaterials. 2009 Feb;30(6):1089-97
references: 19027948
references: Biomaterials. 2010 Jan;31(3):467-80
references: 19815272
references: Science. 1999 Apr 30;284(5415):770-6
references: 10221902
references: Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4872-7
references: 20194780
references: Adv Mater. 2009 Sep 4;21(32-33):3255-68
references: 20882496
references: Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5953-7
references: 15827122
references: Biomaterials. 2002 Aug;23(16):3455-63
references: 12099289
references: Science. 2000 Feb 25;287(5457):1427-30
references: 10688781
references: Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6728-33
references: 10359780
references: Biomacromolecules. 2011 Jun 13;12(6):2193-9
references: 21520924
references: Nano Lett. 2009 Feb;9(2):846-52
references: 19140698
references: Cell. 2006 Aug 25;126(4):677-89
references: 16923388
references: Stem Cells. 2011 Feb;29(2):206-16
references: 21732479
references: J Cell Sci. 2005 Feb 15;118(Pt 4):665-72
references: 15701923
references: Angew Chem Int Ed Engl. 2012 Jun 25;51(26):6411-5
references: 22644619
references: Nature. 2011 Oct 19;478(7369):364-8
references: 22012394
references: Biomaterials. 2011 Jul;32(21):4744-52
references: 21507480
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Science. 2009 Jun 26;324(5935):1673-7
references: 19556500
references: Nat Mater. 2007 Dec;6(12):997-1003
references: 17891143
references: Cell. 1992 Apr 3;69(1):11-25
references: 1555235
references: J Biomed Mater Res A. 2004 Mar 1;68(3):458-72
references: 14762925
references: J R Soc Interface. 2008 Oct 6;5(27):1231-42
references: 18348958
references: Angew Chem Int Ed Engl. 2009;48(37):6790-810
references: 19662666
references: Biomaterials. 2003 Nov;24(24):4385-415
references: 12922151