title: Proposal of screening method for intestinal mucus adhesive lactobacilli using the enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
abstract: Adhesion tests are complex, time-consuming and expensive, while the most important criterion for a probiotic lactobacilli is the ability to adhere to the human intestine. Thirty lactobacilli isolates from human intestinal tissues were measured for cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity using a microtiter plate screening method. GAPDH activities were detected in 21 out of 30 samples from 12 h cultures and in all samples from 18 h cultures. This suggests GAPDH is universally expressed on the bacterial cell surfaces from many lactobacilli. A statistically significant positive correlation was shown between GAPDH activity and adhesion using the BIACORE adhesion assay (P < 0.01). The new screening method using GAPDH enzymatic activity without an adhesion test may be possible due to the significant positive correlation of GAPDH activity with adhesion of lactobacilli derived from the human intestine.
authors: Department of Food Management, School of Food, Agricultural and Environmental Sciences, Miyagi University, Miyagi, Japan. kinoshih@myu.ac.jp
authors: Hideki
authors: H
authors: Kinoshita
authors: Shun
authors: S
authors: Imoto
authors: Yoshihito
authors: Y
authors: Suda
authors: Mitsuharu
authors: M
authors: Ishida
authors: Masamichi
authors: M
authors: Watanabe
authors: Yasushi
authors: Y
authors: Kawai
authors: Haruki
authors: H
authors: Kitazawa
authors: Koh
authors: K
authors: Miura
authors: Akira
authors: A
authors: Horii
authors: Tadao
authors: T
authors: Saito
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001431:Bacteriological Techniques / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D007778:Lactobacillus / Q000201:enzymology* / Q000502:physiology*; D010446:Peptide Fragments / Q000032:analysis*
chemical_list: D010446:Peptide Fragments; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D005987:Glyceraldehyde-3-Phosphate Dehydrogenases