title: Slide drug susceptibility test for the detection of multidrug-resistant tuberculosis in Bangladesh.
abstract: The present study attempted to comparatively assess and establish a suitable detection method of multidrug-resistant tuberculosis (MDR-TB) from previously treated TB cases in Bangladesh. Of 130 Zeihl-Neelsen smear-positive fresh sputum specimens, 112 samples were found to contain viable bacilli as visualized under the light-emitting diode fluorescence microscope after fluorescein di-acetate staining, and 109 positive cases were detected through Löwenstein-Jensen culture. The samples were further tested to survey the drug resistance both by slide drug susceptibility test (DST) and by conventional DST: 94 MDR-TB cases were detected within 10 days through the slide DST, whereas 82 cases were observed through the conventional DST, requiring about 3 months. Because the rapidity, sensitivity and accuracy of the slide DST method were found to be comparatively satisfactory when compared to the conventional DST method; we recommend the slide DST method as the standard diagnostic tool in perspective of Bangladesh for the detection of MDR-TB.
authors: Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka, 1217, Bangladesh, noor.rashed@yahoo.com.
authors: Rashed
authors: R
authors: Noor
authors: Akter
authors: A
authors: Hossain
authors: Saurab Kishore
authors: SK
authors: Munshi
authors: Farjana
authors: F
authors: Rahman
authors: S M Mostofa
authors: SM
authors: Kamal
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001459:Bangladesh; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents