title: Configuration of biological wastewater treatment line and influent composition as the main factors driving bacterial community structure of activated sludge.
abstract: The structure of microbial consortia in wastewater treatment facilities is a resultant of environmental conditions created by the operational parameters of the purification process. In the research, activated sludge from nine Polish wastewater treatment plants (WWTPs) was investigated at a molecular level to determine the impact of the complexity of biological treatment line and the influent composition on the species structure and the diversity of bacterial consortia. The community fingerprints and technological data were subjected to the canonical correspondence and correlation analyses. The number of separated biological processes realized in the treatment line and the presence of industrial wastewater in the influent were the key factors determining the species structure of total and ammonia-oxidizing bacteria in biomass. The N2O-reducers community composition depended significantly on the design of the facility; the highest species richness of denitrifiers was noted in the WWTPs with separated denitrification tanks. The contribution of industrial streams to the inflow affected the diversity of total and denitrifying bacterial consortia and diminished the diversity of ammonia oxidizers. The obtained data are valuable for engineers since they revealed the main factors, including the design of wastewater treatment plant, influencing the microbial groups critical for the stability of purification processes.
authors: Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, Olsztyn, Poland.
authors: Paulina
authors: P
authors: Jaranowska
authors: Agnieszka
authors: A
authors: Cydzik-Kwiatkowska
authors: Magdalena
authors: M
authors: Zielińska
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D058448:Biota; D054892:Metagenome; D008969:Molecular Sequence Data; D011044:Poland; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D012722:Sewage
references: FEMS Microbiol Ecol. 2004 Sep 1;49(3):401-17
references: 19712290
references: Water Res. 2011 Oct 1;45(15):4390-8
references: 21705039
references: Environ Microbiol. 2007 Sep;9(9):2220-33
references: 17686020
references: Environ Microbiol. 2002 Mar;4(3):158-68
references: 12000316
references: Microbiol Mol Biol Rev. 1997 Dec;61(4):533-616
references: 9409151
references: Water Res. 2011 Jan;45(3):1129-38
references: 21112606
references: Environ Microbiol. 2007 Dec;9(12):2993-3007
references: 17991028
references: Bioresour Technol. 2011 Feb;102(4):3694-701
references: 21185720
references: Water Res. 2007 Mar;41(5):1110-20
references: 17254627
references: Appl Environ Microbiol. 1997 Dec;63(12):4704-12
references: 9406389
references: Appl Environ Microbiol. 1999 Aug;65(8):3697-704
references: 10427068
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: Mol Biol Evol. 1993 May;10(3):512-26
references: 8336541
references: J Environ Sci (China). 2010;22(4):627-34
references: 20617742
references: Pol J Microbiol. 2012;61(1):41-9
references: 22708345
references: Arch Microbiol. 2002 Feb;177(2):139-49
references: 11807563
references: Bioresour Technol. 2010 Dec;101(23):8975-83
references: 20674347
references: FEMS Microbiol Ecol. 2005 Oct 1;54(2):205-17
references: 16332320
references: Arch Microbiol. 1984 Jan;137(1):74-8
references: 6712418
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Appl Environ Microbiol. 1993 Mar;59(3):695-700
references: 7683183
references: Chemosphere. 2009 Apr;75(2):234-42
references: 19246073
references: Sci Total Environ. 2009 Jun 15;407(13):3994-4003
references: 19394070
references: Folia Microbiol (Praha). 2011 May;56(3):201-8
references: 21618047