title: Vesicular stomatitis virus with the rabies virus glycoprotein directs retrograde transsynaptic transport among neurons in vivo.
abstract: Defining the connections among neurons is critical to our understanding of the structure and function of the nervous system. Recombinant viruses engineered to transmit across synapses provide a powerful approach for the dissection of neuronal circuitry in vivo. We recently demonstrated that recombinant vesicular stomatitis virus (VSV) can be endowed with anterograde or retrograde transsynaptic tracing ability by providing the virus with different glycoproteins. Here we extend the characterization of the transmission and gene expression of recombinant VSV (rVSV) with the rabies virus glycoprotein (RABV-G), and provide examples of its activity relative to the anterograde transsynaptic tracer form of rVSV. rVSV with RABV-G was found to drive strong expression of transgenes and to spread rapidly from neuron to neuron in only a retrograde manner. Depending upon how the RABV-G was delivered, VSV served as a polysynaptic or monosynaptic tracer, or was able to define projections through axonal uptake and retrograde transport. In animals co-infected with rVSV in its anterograde form, rVSV with RABV-G could be used to begin to characterize the similarities and differences in connections to different areas. rVSV with RABV-G provides a flexible, rapid, and versatile tracing tool that complements the previously described VSV-based anterograde transsynaptic tracer.
authors: Department of Genetics and Department of Ophthalmology, Harvard Medical School, Harvard University and Howard Hughes Medical Institute Boston, MA, USA.
authors: Kevin T
authors: KT
authors: Beier
authors: Arpiar B
authors: AB
authors: Saunders
authors: Ian A
authors: IA
authors: Oldenburg
authors: Bernardo L
authors: BL
authors: Sabatini
authors: Constance L
authors: CL
authors: Cepko
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D057809:HEK293 Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000502:physiology* / Q000821:virology; D009924:Organ Culture Techniques; D021381:Protein Transport / Q000502:physiology; D011820:Rabies virus / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000737:chemistry / Q000502:physiology* / Q000821:virology; D014759:Viral Envelope Proteins / Q000502:physiology*
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins
keywords: in vivo; polysynaptic; rabies; retrograde transneuronal tracing; technology; transsynaptic infection; vesicular stomatitis virus
references: Science. 1994 Jan 14;263(5144):232-4
references: 8284675
references: Trends Neurosci. 1995 Feb;18(2):63-4
references: 7537410
references: J Neurosci. 2003 Sep 10;23(23):8432-44
references: 12968006
references: J Comp Neurol. 2009 Oct 20;516(6):456-81
references: 19672982
references: Science. 2005 Jun 10;308(5728):1643-5
references: 15831716
references: J Interferon Cytokine Res. 2009 Sep;29(9):499-509
references: 19715459
references: Nat Med. 2000 Jul;6(7):821-5
references: 10888934
references: Nat Methods. 2007 Jan;4(1):47-9
references: 17179932
references: Nature. 2007 Nov 1;450(7166):110-4
references: 17934446
references: J Virol. 2002 Feb;76(3):1309-27
references: 11773406
references: J Virol. 1998 Oct;72(10):8413-9
references: 9733895
references: J Virol. 1987 Mar;61(3):812-8
references: 2433468
references: J Virol. 2001 Dec;75(24):12169-81
references: 11711608
references: Infect Immun. 1981 Jan;31(1):297-308
references: 6163717
references: J Infect Dis. 2011 Nov;204 Suppl 3:S1075-81
references: 21987744
references: J Comp Neurol. 1995 Jun 5;356(3):457-80
references: 7642806
references: Neuron. 2012 Jun 7;74(5):858-73
references: 22681690
references: Nat Neurosci. 2010 Jan;13(1):133-40
references: 20023653
references: Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21848-53
references: 21115815
references: Virology. 2007 Sep 15;366(1):166-73
references: 17524441
references: J Virol. 2004 Sep;78(17):9317-24
references: 15308726
references: J Virol. 2006 Mar;80(6):2675-83
references: 16501077
references: J Neurosci Methods. 2000 Nov 15;103(1):63-71
references: 11074096
references: Vaccine. 2009 Feb 5;27(6):893-903
references: 19070640
references: Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8388-92
references: 7667300
references: Neuroscience. 2007 Dec 12;150(3):639-46
references: 17961930
references: Mol Cell Biol. 2000 Nov;20(22):8590-601
references: 11046154
references: Nature. 2011 Mar 10;471(7337):177-82
references: 21390124
references: Am J Public Health Nations Health. 1957 Feb;47(2):205-9
references: 13394773
references: Nature. 2011 Mar 10;471(7337):183-8
references: 21390125
references: Cancer Cell. 2003 Oct;4(4):263-75
references: 14585354
references: J Neurosci Methods. 1991 Apr;37(2):173-82
references: 1715499
references: Mol Cell. 2000 Nov;6(5):1243-52
references: 11106761
references: Virus Res. 2002 May 10;85(2):211-9
references: 12034487
references: Brain Res. 1993 Aug 13;619(1-2):146-56
references: 7690671
references: Nat Med. 2000 May;6(5):595-8
references: 10802719
references: Am J Epidemiol. 1967 Jul;86(1):158-61
references: 4378113
references: Science. 1989 Jan 6;243(4887):89-91
references: 2536188
references: Virology. 1987 Sep;160(1):236-45
references: 2820131
references: Virology. 2005 Sep 30;340(2):174-82
references: 16043204
references: Hum Mol Genet. 2001 Sep 15;10(19):2109-21
references: 11590128
references: Nat Rev Immunol. 2006 Mar;6(3):231-43
references: 16498452
references: Science. 1997 Jun 20;276(5320):1845-8
references: 9188527
references: J Interferon Res. 1983;3(4):425-35
references: 6323591
references: J Virol. 1992 Jul;66(7):4058-64
references: 1318397
references: Synapse. 2000 Sep 15;37(4):252-61
references: 10891862
references: Neuron. 2004 Oct 28;44(3):483-93
references: 15504328
references: Nature. 2010 Jun 24;465(7301):1079-83
references: 20577213
references: Virology. 2010 May 10;400(2):187-96
references: 20172575
references: IUBMB Life. 2000 Aug;50(2):135-8
references: 11185959
references: Am J Trop Med Hyg. 1966 Mar;15(2):244-6
references: 4286381
references: J Comp Neurol. 1984 Feb 10;223(1):46-56
references: 6200513
references: J Gen Virol. 1982 Jul;61 (Pt l):97-100
references: 6181189
references: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6716-21
references: 10841568
references: Virology. 1983 Nov;131(1):137-43
references: 6316647
references: Neuron. 2007 Mar 1;53(5):639-47
references: 17329205
references: Trends Mol Med. 2004 May;10(5):210-6
references: 15121047
references: J Virol. 2000 Nov;74(21):9818-27
references: 11024108
references: Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9
references: 21825165