title: Performance and mechanism of neuroleukin in the growth and survival of sertoli cell-induced neurons in a coculture system.
abstract: Sertoli cells (SCs), which are recognized as the "nurse cells" of the testis due to their important biofunctions, have been used in cotransplantation with neurons in cell therapy. However, it is not clear whether SCs influence neuronal communication and survival. In this study, we showed that approximately 60% of cortical neural stem cells (NSCs) cocultured with SCs differentiated into mature neurons. In addition, the neurite outgrowth and neuronal survival rates were significantly enhanced in the coculture system compared with differentiated neurons induced by a differentiation medium. The neuroleukin (NLK) secretion of SCs was also identified at the RNA and protein level, and the roles of NLK in neuromorphology and physiological regulation were systematically investigated for the first time. These results not only highlight the significance of paracrine regulation of NSCs by SCs but also confirm the role NLK plays in the differentiation and survival of NSCs. Finally, we proposed a possible hypothesis for the mechanism of NLK in the growth and survival of SC-induced neurons based on Western blotting results, which is that NLK secreted by SCs activates the Ras/Raf/MEK/Erk, Jak/Stat, and PI3K/Akt pathways, but not the NF-κB pathway, in neurons resulting in their growth and survival.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, P.R. China.
authors: Lei
authors: L
authors: Deng
authors: Bingyang
authors: B
authors: Shi
authors: Yingping
authors: Y
authors: Zhuang
authors: Ju
authors: J
authors: Chu
authors: Xiaolin
authors: X
authors: Shi
authors: Siliang
authors: S
authors: Zhang
authors: Meijin
authors: M
authors: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis / Q000378:metabolism*; D053613:Janus Kinase 1 / Q000378:metabolism; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D053613:Janus Kinase 1; D051057:Proto-Oncogene Proteins c-akt; D048369:MAP Kinase Kinase 1; D005956:Glucose-6-Phosphate Isomerase