title: Differentiation of human adipose-derived stem cells co-cultured with urothelium cell line toward a urothelium-like phenotype in a nude murine model.
abstract: OBJECTIVE
  To investigated the urothelium differentiation potential of adipose-derived stem cells (ASCs) that were coimplanted with the immortalized human bladder urothelium cell line (SV-HUC-1) into the subcutaneous tissue of athymic mice.
  
  
  METHODS
  The ASCs were isolated from the human adipose tissue of patients undergoing liposuction procedures and were expanded in vitro. After labeling with CM-DiI, the ASCs were mixed with SV-HUC-1 and implanted into the subcutaneous tissue of athymic mice for 2 and 4 weeks. The urothelium-specific markers uroplakin-Ia and uroplakin-II were detected by immunofluorescence. The transformation rate of ASCs into the urothelium phenotype was evaluated at each measurement point.
  
  
  RESULTS
  We found that 25.87% ± 1.38% of ASCs expressed the urothelium-specific marker uroplakin-Ia and 23.60% ± 2.57% of ASCs expressed uroplakin-II 2 weeks after coimplantation with SV-HUC-1 in vivo. After 4 weeks, 70.07% ± 3.84% of ASCs expressed uroplakin-Ia and 65.56% ± 2.94% expressed uroplakin-II. However, no obvious organizational multilayered urothelium structure, such as that of the native bladder mucosa, was found in the subcutaneous tissues of the athymic mice.
  
  
  CONCLUSIONS
  The results of our study have demonstrated that ASCs could be differentiated toward the urothelium-like phenotype when they were coimplanted in direct contact with cells of a mature urothelium cell line, and the proportion of differentiated cells increased from 2 to 4 weeks. The differentiation potential of ASCs toward the urothelial cell type suggests that ASCs might have potential to be used in urinary tract repair with a tissue engineering approach in the future.
authors: Department of Urology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Ming
authors: M
authors: Zhang
authors: Yubing
authors: Y
authors: Peng
authors: Zhe
authors: Z
authors: Zhou
authors: Juan
authors: J
authors: Zhou
authors: Zhong
authors: Z
authors: Wang
authors: Mujun
authors: M
authors: Lu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication; D002454:Cell Differentiation; D018920:Coculture Techniques; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D060170:Uroplakin II / Q000378:metabolism; D060167:Uroplakin Ia / Q000378:metabolism; D019459:Urothelium / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers; C554658:UPK2 protein, human; D060170:Uroplakin II; D060167:Uroplakin Ia