title: Epidemic plasmid carrying bla(CTX-M-15) in Klebsiella penumoniae in China.
abstract: OBJECTIVE
  To investigate the local epidemiology of Klebsiella penumoniae carrying bla(CTX-M-15) in southern China and to characterize the genetic environment of bla(CTX-M-15).
  
  
  METHODS
  PCR and DNA sequencing were used to detect and characterize the genetic contexts of bla(CTX-M-15). The clonal relatedness of isolates carrying bla(CTX-M-15) was determined by pulse-field gel electrophoresis. Conjugative plasmids carrying bla(CTX-M-15) were obtained by mating and were further subject to restriction analysis and replicon typing.
  
  
  RESULTS
  A total of 47CTX-M-15 ESBL-producing isolates of K. pneumoniae were collected from nine hospitals in China from October 2007 to October 2008. Isolates were clustered into various clonal groups. The local spread of bla(CTX-M-15) was mainly mediated by one major conjugative plasmid as determined by S1-PFGE and restriction analysis. A 90-kb plasmid belonging to incompatible group FII was the major carrier of bla(CTX-M-15) in K. pneumoniae. Except bla(TEM-1), the resistance genes such as bla(SHV), bla(DHA-1), bla(OXA-1), qnrB, qnrS, aac(3)-II, and aac(6')-Ib were not found in the plasmid. In the comparing of conjugative gene sequence, it is 100% identical with the plasmid pKF3-94, which was found in K. pneumonia from Zhejiang province of china previously.
  
  
  CONCLUSIONS
  bla(CTX-M-15) was prevalent in K. pneumonia of southern China. The dissemination of bla(CTX-M-15) appeared to be due to the horizontal transfer of a 90-kb epidemic plasmid.
authors: State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, Guangzhou, China. chao_sheep@263.net
authors: Chao
authors: C
authors: Zhuo
authors: Xiao-qiang
authors: XQ
authors: Li
authors: Zhi-yong
authors: ZY
authors: Zong
authors: Nan-Shan
authors: NS
authors: Zhong
mesh_terms: D001483:Base Sequence; D002681:China; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D022761:Gene Transfer, Horizontal; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases
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