title: Assessment of the prevalence and diversity of emergent campylobacteria in human stool samples using a combination of traditional and molecular methods.
abstract: This study aims to assess the diversity of campylobacteria (Campylobacter and Arcobacter) in human fecal samples from patients with diarrhea (n = 140) and asymptomatic controls (n = 116) in Chile, using a combination of traditional culture and molecular methods. The culture methods detected campylobacteria in 10.7% of the patients with diarrhea and in 1.7% of the controls. In contrast, the molecular methods detected campylobacteria more often than the traditional culture, with a prevalence of 25.7% and 5.2%, respectively. The traditional methods only recovered the species Campylobacter jejuni, Campylobacter coli, and Arcobacter butzleri, whereas the molecular methods additionally detected the emergent species Campylobacter concisus and Campylobacter ureolyticus.
authors: Institute of Clinical Microbiology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile. luiscollado@uach.cl
authors: Luis
authors: L
authors: Collado
authors: Magali
authors: M
authors: Gutiérrez
authors: Mario
authors: M
authors: González
authors: Heriberto
authors: H
authors: Fernández
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020640:Arcobacter / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods*; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D002677:Chile; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D005260:Female; D014644:Genetic Variation; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D055815:Young Adult