title: Laserspray and matrix-assisted ionization inlet coupled to high-field FT-ICR mass spectrometry for peptide and protein analysis.
abstract: We present the first coupling of laser spray ionization inlet (LSII) and matrix assisted ionization inlet (MAII) to high-field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) for generation of electrospray-like ions to take advantage of increased sensitivity, mass range, and mass resolving power afforded by multiple charging. We apply the technique to top-down protein analysis and characterization of metalloproteins. We also present a novel method for generation of multiply-charged copper-peptide complexes with varying degrees of copper adduction by LSII. We show an application of the generated copper-peptide complexes for protein charge state and molecular weight determination, particularly useful for an instrument such as a linear ion trap mass analyzer.
authors: National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310, USA.
authors: Leonard
authors: L
authors: Nyadong
authors: Ellen D
authors: ED
authors: Inutan
authors: Xu
authors: X
authors: Wang
authors: Christopher L
authors: CL
authors: Hendrickson
authors: Sarah
authors: S
authors: Trimpin
authors: Alan G
authors: AG
authors: Marshall
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D056831:Coordination Complexes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D017357:Cyclotrons; D004867:Equipment Design; D005583:Fourier Analysis; D006801:Humans; D008667:Metalloproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D056831:Coordination Complexes; D008667:Metalloproteins; D010455:Peptides; D003300:Copper
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