title: Immunoexpression of DIABLO, AIF and cytochrome C in gastric adenocarcinoma assessed by tissue Microarray.
abstract: The aim of this study was to analyze the immunoexpression of (Smac) DIABLO, AIF, cytochrome c, Ki-67 and cleaved caspase-3 in gastric cancer. A tissue microarray (TMA) paraffin block was constructed using gastric adenocarcinoma tissue and adjacent normal adjacent mucosa from 87 patients who had not previously undergone radiotherapy or chemotherapy. Immunohistochemistry was used to evaluate the protein levels. Samples were positive for (Smac) DIABLO in 37 (45.6%) and 37 (46.8%), for AIF in 31 (36.9%) and 36 (45.6%), for cytochrome c in 60 (68.9%) and 44 (54.4%), for Ki-67 in 63 (72.4%) and 52 (61.9%) and for cleaved caspase-3 in 21 (24.1%) and 3 (3.4%) cases of tumor and adjacent normal tissues, respectively. Our results suggest that increased expression of Ki-67 and cleaved caspase-3 could contribute to carcinogenesis. The expression of these proteins indicates an attempt of cells to maintain tissue homeostasis.
authors: DDS, Department of Biosciences, Federal University of São Paulo, UNIFESP, Av Ana Costa 95, Vila Mathias, Santos - SP, ZIP code: 11060-001, Brasil. daribeiro@unifesp.br
authors: Larissa Comparini
authors: LC
authors: DA Silva
authors: Nora Manoukian
authors: NM
authors: Forones
authors: Daniel Araki
authors: DA
authors: Ribeiro
authors: Silvia Sauli Miki
authors: SS
authors: Ihara
authors: Thiago Simão
authors: TS
authors: Gomes
authors: Ricardo Artigiani
authors: RA
authors: Neto
authors: Celina Tizuko Fujiyama
authors: CT
authors: Oshima
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051033:Apoptosis Inducing Factor / Q000032:analysis / Q000096:biosynthesis*; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor / Q000032:analysis; D045304:Cytochromes c / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000096:biosynthesis*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000032:analysis / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality; D046888:Tissue Array Analysis
chemical_list: C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C412403:DIABLO protein, human; D047908:Intracellular Signaling Peptides and Proteins; D024101:Mitochondrial Proteins; D045304:Cytochromes c