title: PLAG1 and CYLD do not play a role in the tumorigenesis of adenoid cystic carcinoma.
abstract: The pleiomorphic adenoma gene 1 (PLAG1) gene is activated in a subset of pleomorphic adenomas of the salivary gland by gene fusion. Germ‑line mutation in cylindromatosis (CYLD), a tumor suppressor gene, causes familial cylindromatosis and Brook‑Spiegler syndrome. In the present study, aberrations in PLAG1 and CYLD were investigated in adenoid cystic carcinoma (ACC) of the salivary gland. Reverse‑transcription PCR and PCR direct sequencing were performed to detect gene fusion of PLAG1 and mutation of CYLD in 34 ACC tissues. No PLAG1 fusion was detected in ACC. However, silent mutation of CYLD was detected in 2 cases of ACC, but no missense mutation was detected in ACC. These results suggest that PLAG1 and CYLD do not play a role in ACC tumorigenesis.
authors: Department of Diagnostic Pathology, Faculty of Medicine, Oita University, Oita 879-5593, Japan. daatom@oita-u.ac.jp
authors: Tsutomu
authors: T
authors: Daa
authors: Itaru
authors: I
authors: Nakamura
authors: Naomi
authors: N
authors: Yada
authors: Shigeki
authors: S
authors: Arakane
authors: Haruto
authors: H
authors: Nishida
authors: Kenji
authors: K
authors: Kashima
authors: Masashi
authors: M
authors: Suzuki
authors: Shigeo
authors: S
authors: Yokoyama
mesh_terms: D063646:Carcinogenesis; D003528:Carcinoma, Adenoid Cystic / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D000074749:Deubiquitinating Enzyme CYLD; D006801:Humans; D009154:Mutation; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D012469:Salivary Glands / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D015514:Oncogene Proteins, Fusion; C104382:PLAG1 protein, human; D025521:Tumor Suppressor Proteins; C409984:CYLD protein, human; D000074749:Deubiquitinating Enzyme CYLD