title: Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2.
abstract: Mammalian SFMBTs have been considered to be polycomb group repressors. However, molecular mechanisms underlying mammalian SFMBTs-mediated gene regulation and their biological function have not been characterized. In the present study, we identified YY1 and methylated histones as interacting proteins of human SFMBT2. We also found that human SFMBT2 binds preferentially to methylated histone H3 and H4 that are associated with transcriptional repression. Using DU145 prostate cancer cells as a model, we showed that SFMBT2 has a transcriptional repression activity on HOXB13 gene expression. In addition, occupancy of SFMBT2 coincided with enrichment of diand tri-methylated H3K9 and H4K20 as well as tri-methylated H3K27 at the HOXB13 gene promoter. When SFMBT2 was depleted by siRNA in DU145 prostate cancer cells, significant up-regulation of HOXB13 gene expression and decreased cell growth were observed. Collectively, our findings indicate that human SFMBT2 may regulate cell growth via epigenetic regulation of HOXB13 gene expression in DU145 prostate cancer cells.
authors: Department of Life Science, Sogang University, Seoul 121-742, Korea.
authors: Kwanghyun
authors: K
authors: Lee
authors: Wonho
authors: W
authors: Na
authors: Je-Heon
authors: JH
authors: Maeng
authors: Hongjin
authors: H
authors: Wu
authors: Bong-Gun
authors: BG
authors: Ju
mesh_terms: D045744:Cell Line, Tumor; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C484414:HOXB13 protein, human; D006657:Histones; D018398:Homeodomain Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C582334:SFMBT2 protein, human
references: J Biol Chem. 2005 Jul 1;280(26):24428-34
references: 15870067
references: Gene Expr Patterns. 2008 Jan;8(2):107-16
references: 18024232
references: FEBS Lett. 2007 Jul 10;581(17):3289-96
references: 17599839
references: Nature. 2004 Oct 14;431(7010):873-8
references: 15386022
references: Cancer Res. 2004 Dec 15;64(24):9185-92
references: 15604291
references: BMC Genomics. 2009 Mar 31;10:143
references: 19335899
references: Nature. 2002 Oct 10;419(6907):624-9
references: 12374981
references: Genes Dev. 2006 May 1;20(9):1110-22
references: 16618800
references: Trends Biochem Sci. 2003 Feb;28(2):69-74
references: 12575993
references: Am J Pathol. 2007 Apr;170(4):1370-8
references: 17392175
references: Semin Cell Dev Biol. 2010 Apr;21(2):221-30
references: 19778625
references: Cancer Res. 2004 May 1;64(9):3046-51
references: 15126340
references: Mol Cell Biol. 1999 Oct;19(10):7237-44
references: 10490658
references: Brief Funct Genomics. 2010 Dec;9(5-6):429-43
references: 21266346
references: Yonsei Med J. 2011 May;52(3):463-8
references: 21488189
references: Genes Dev. 2007 Jan 1;21(1):49-54
references: 17210787
references: Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11606-11
references: 14500907
references: Clin Cancer Res. 2004 May 1;10(9):3195-206
references: 15131061
references: Int J Biochem Cell Biol. 2009 May;41(5):1094-101
references: 19013255
references: EMBO J. 2009 Jul 8;28(13):1965-77
references: 19494831
references: Cell Stem Cell. 2010 Dec 3;7(6):682-93
references: 21112563
references: Trends Biochem Sci. 2010 Jun;35(6):323-32
references: 20346678
references: BMC Dev Biol. 2007 Feb 15;7:8
references: 17302988
references: Genes Dev. 2004 Jul 1;18(13):1592-605
references: 15231737
references: Int J Oncol. 2005 Jul;27(1):131-41
references: 15942652
references: Cell. 2004 Jun 25;117(7):859-72
references: 15210108
references: Oncogene. 2006 Feb 23;25(8):1125-42
references: 16314846