title: Esophageal human β-defensin expression in eosinophilic esophagitis.
abstract: BACKGROUND
  Defensins are antimicrobial peptides expressed on mucosal surfaces that contribute to maintaining intestinal homeostasis by providing innate defense mechanisms for the epithelia. Defensin expression is altered in a number of diseases that affect mucosal surfaces, such as atopic dermatitis, allergic rhinitis, and inflammatory bowel disease. Similar to atopic dermatitis, eosinophilic esophagitis (EoE) is a chronic disease in which the squamous epithelial surface is affected by a similar TH2 microenvironment and eosinophil-predominant inflammation. Therefore, we hypothesized that defensin expression would be decreased in EoE.
  
  
  METHODS
  To address this, we measured defensin expression in vitro in cell lines derived from patients with EoE (EoE1-T) or gastroesophageal reflux disease (GERD) (NES-G4T cells) and ex vivo in esophageal mucosal biopsy samples from children with EoE or GERD and control children without esophageal disease.
  
  
  RESULTS
  Interleukin-5 induced a decrease in human β-defensin (hBD) -1 and hBD3 expression in EoE1-T but not in NES-G4T cells. Compared with esophageal biopsy specimens from GERD and control children, specimens from EoE pediatric patients revealed a significant decrease in mRNA and protein expression for hBD1 and hBD3.
  
  
  CONCLUSIONS
  Diminished expression of hBD1 and hBD3 may make the esophageal epithelium more susceptible to the development and/or perpetuation of EoE.
authors: Department of Pediatrics, Gastrointestinal Eosinophilic Diseases Program, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors: Shauna
authors: S
authors: Schroeder
authors: Zachary D
authors: ZD
authors: Robinson
authors: Joanne C
authors: JC
authors: Masterson
authors: Lindsay
authors: L
authors: Hosford
authors: Wendy
authors: W
authors: Moore
authors: Zhaoxing
authors: Z
authors: Pan
authors: Rachel
authors: R
authors: Harris
authors: Rhonda F
authors: RF
authors: Souza
authors: Stuart Jon
authors: SJ
authors: Spechler
authors: Sophie A
authors: SA
authors: Fillon
authors: Glenn T
authors: GT
authors: Furuta
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D057765:Eosinophilic Esophagitis / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism*; D006801:Humans; D009092:Mucous Membrane / Q000378:metabolism; D055815:Young Adult; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: D023083:beta-Defensins
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