title: Paired pulse ratio analysis of insulin-induced synaptic plasticity in the snail brain.
abstract: Insulin's action in the brain can directly alter cognitive functioning. We have recently shown that molluscan insulin-related peptides are upregulated following a conditioned taste aversion (CTA) training procedure. In addition, when mammalian insulin is superfused over the isolated Lymnaea stagnalis central nervous system, it elicits long-term synaptic enhancement at the monosynaptic connection between the cerebral giant cell and the buccal 1 (B1) motor neuron. This synaptic enhancement is thought to be a neural correlate of CTA. Here, we examined whether the observed changes in synaptic plasticity were the result of presynaptic and/or postsynaptic alterations using the paired pulse procedure. The paired pulse ratio was unaltered following insulin application, suggesting that insulin's effects on synaptic plasticity are mediated postsynaptically in the B1 motor neuron. Thus, it was suggested that postsynaptic changes need to be considered when insulin's actions on synaptic plasticity are examined.
authors: Laboratory of Functional Biology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Japan.
authors: Jun
authors: J
authors: Murakami
authors: Ryuichi
authors: R
authors: Okada
authors: Yutaka
authors: Y
authors: Fujito
authors: Manabu
authors: M
authors: Sakakibara
authors: Ken
authors: K
authors: Lukowiak
authors: Etsuro
authors: E
authors: Ito
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D007328:Insulin / Q000494:pharmacology*; D008195:Lymnaea / Q000187:drug effects / Q000502:physiology*; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009473:Neuronal Plasticity / Q000187:drug effects*; D013569:Synapses / Q000187:drug effects / Q000502:physiology*
chemical_list: D007328:Insulin
keywords: Lymnaea; conditioned taste aversion; insulin; paired pulse ratio; synaptic plasticity