title: Nicotinamide downregulates gene expression of interleukin-6, interleukin-10, monocyte chemoattractant protein-1, and tumour necrosis factor-α gene expression in HaCaT keratinocytes after ultraviolet B irradiation.
abstract: Ultraviolet (UV) radiation has profound effects on human skin, causing sunburn, inflammation, cellular-tissue injury, cell death, and skin cancer. Most of these effects are mediated by a number of cytokines produced by keratinocytes. In this study we investigated whether nicotinamide (NCT), the amide form of vitamin B3, might have a protective function in reducing the expression of interleukin (IL)-1β, IL-6, IL-8, IL-10, monocyte chemoattractant protein (MCP)-1 and tumour necrosis factor (TNF)-α in UV-irradiated keratinocytes. HaCaT cells were treated with UVB in the presence or absence of NCT, and cytokine mRNA levels were examined by quantitative real-time PCR. NCT significantly downregulated IL-6, IL-10, MCP-1 and TNF-α mRNA expression, whereas it did not exert any significant effect on IL-1β or IL-8 expression. Because of its ability to decrease these cytokine mediators after UV exposure, NCT is a possible therapy to improve or prevent conditions induced or aggravated by UV light.
authors: Department of Dermatology, University of Naples Federico II, Naples, Italy.
authors: G
authors: G
authors: Monfrecola
authors: F
authors: F
authors: Gaudiello
authors: T
authors: T
authors: Cirillo
authors: G
authors: G
authors: Fabbrocini
authors: A
authors: A
authors: Balato
authors: S
authors: S
authors: Lembo
mesh_terms: D018932:Chemokine CCL2 / Q000235:genetics; D016207:Cytokines / Q000378:metabolism*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D009536:Niacinamide / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014466:Ultraviolet Rays; D014803:Vitamin B Complex / Q000494:pharmacology*
chemical_list: D018932:Chemokine CCL2; D016207:Cytokines; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D014803:Vitamin B Complex; D016753:Interleukin-10; D009536:Niacinamide