title: Development of PCR assays for detection of Trichomonas vaginalis in urine specimens.
abstract: Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans.
authors: Centers for Disease Control and Prevention, Atlanta, Georgia, USA. cbandea@cdc.gov
authors: Claudiu I
authors: CI
authors: Bandea
authors: Kahaliah
authors: K
authors: Joseph
authors: Evan W
authors: EW
authors: Secor
authors: Laurie A
authors: LA
authors: Jones
authors: Joseph U
authors: JU
authors: Igietseme
authors: Robert L
authors: RL
authors: Sautter
authors: Margaret R
authors: MR
authors: Hammerschlag
authors: Nancy N
authors: NN
authors: Fajman
authors: Rebecca G
authors: RG
authors: Girardet
authors: Carolyn M
authors: CM
authors: Black
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014245:Trichomonas Infections / Q000175:diagnosis*; D014246:Trichomonas vaginalis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014556:Urine / Q000469:parasitology*
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