title: Structure determination from a single high-pressure-frozen virus crystal.
abstract: Successful cryogenic X-ray structure determination from a single high-pressure-frozen bovine enterovirus 2 crystal is reported. The presented high-pressure-freezing procedure is based on a commercially available device and allows the cryocooling of macromolecular crystals directly in their mother liquor without the time- and crystal-consuming search for optimal cryoconditions. The method is generally applicable and will allow cryogenic data collection from all types of macromolecular crystals.
authors: Deutsches Elektronen-Synchrotron (DESY), Notkestrasse 85, 22607 Hamburg, Germany.
authors: Anja
authors: A
authors: Burkhardt
authors: Armin
authors: A
authors: Wagner
authors: Martin
authors: M
authors: Warmer
authors: Rudolph
authors: R
authors: Reimer
authors: Heinrich
authors: H
authors: Hohenberg
authors: Jingshan
authors: J
authors: Ren
authors: Elizabeth E
authors: EE
authors: Fry
authors: David I
authors: DI
authors: Stuart
authors: Alke
authors: A
authors: Meents
mesh_terms: D000818:Animals; D002417:Cattle; D020285:Cryoelectron Microscopy / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004058:Diffusion; D029650:Enterovirus, Bovine / Q000737:chemistry* / Q000528:radiation effects; D005615:Freezing / Q000009:adverse effects; D011312:Pressure
chemical_list: D003451:Cryoprotective Agents
keywords: cryocooling; high-pressure-freezing; virology