title: Deep sympatric mtDNA divergence in the autumnal moth (Epirrita autumnata).
abstract: Deep sympatric intraspecific divergence in mtDNA may reflect cryptic species or formerly distinct lineages in the process of remerging. Preliminary results from DNA barcoding of Scandinavian butterflies and moths showed high intraspecific sequence variation in the autumnal moth, Epirrita autumnata. In this study, specimens from different localities in Norway and some samples from Finland and Scotland, with two congeneric species as outgroups, were sequenced with mitochondrial and nuclear markers to resolve the discrepancy found between mtDNA divergence and present species-level taxonomy. We found five COI sub-clades within the E. autumnata complex, most of which were sympatric and with little geographic structure. Nuclear markers (ITS2 and Wingless) showed little variation and gave no indications that E. autumnata comprises more than one species. The samples were screened with primers for Wolbachia outer surface gene (wsp) and 12% of the samples tested positive. Two Wolbachia strains were associated with different mtDNA sub-clades within E. autumnata, which may indicate indirect selection/selective sweeps on haplotypes. Our results demonstrate that deep mtDNA divergences are not synonymous with cryptic speciation and this has important implications for the use of mtDNA in species delimitation, like in DNA barcoding.
authors: Natural History Museum, University of Oslo P.O. Box 1172, Blindern, N-0318, Oslo, Norway ; Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science P.O. Box 8146 Dep, N-0033, Oslo, Norway.
authors: Kjersti S
authors: KS
authors: Kvie
authors: Silje
authors: S
authors: Hogner
authors: Leif
authors: L
authors: Aarvik
authors: Jan T
authors: JT
authors: Lifjeld
authors: Arild
authors: A
authors: Johnsen
keywords: DNA barcoding; Epirrita autumnata; Wolbachia; mtDNA; nDNA; selective sweep
references: Syst Biol. 2004 Apr;53(2):278-98
references: 15205053
references: Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1905-16
references: 16214748
references: Mol Ecol. 2007 Sep;16(17):3617-33
references: 17845435
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Annu Rev Entomol. 1997;42:587-609
references: 15012323
references: Genetics. 1992 Jun;131(2):479-91
references: 1644282
references: Genetics. 1989 Nov;123(3):585-95
references: 2513255
references: Nat Commun. 2011;2:324
references: 21610727
references: Theor Popul Biol. 1975 Apr;7(2):256-76
references: 1145509
references: J Mol Evol. 1980 Dec;16(2):111-20
references: 7463489
references: J Anim Ecol. 2008 Mar;77(2):257-64
references: 18070041
references: Mol Phylogenet Evol. 2009 Mar;50(3):485-95
references: 19056502
references: Mol Phylogenet Evol. 2001 Mar;18(3):467-78
references: 11277638
references: Heredity (Edinb). 2009 Jun;102(6):590-9
references: 19293835
references: PLoS Biol. 2004 Oct;2(10):e286
references: 15361935
references: Neotrop Entomol. 2009 Jul-Aug;38(4):441-51
references: 19768260
references: Mol Biol Evol. 1992 Jul;9(4):678-87
references: 1630306
references: Bioinformatics. 2009 Jun 1;25(11):1451-2
references: 19346325
references: Philos Trans R Soc Lond B Biol Sci. 1986 Jan 29;312(1154):325-42
references: 2870525
references: Mol Ecol Resour. 2009 Sep;9(5):1302-10
references: 21564901
references: PLoS One. 2012;7(1):e30490
references: 22272356
references: J Mol Evol. 1995 Sep;41(3):353-8
references: 7563121
references: Heredity (Edinb). 2001 Sep;87(Pt 3):294-304
references: 11737276
references: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):968-71
references: 16418261
references: Genetics. 2003 Dec;165(4):1959-69
references: 14704179
references: Genetics. 2002 Mar;160(3):1075-86
references: 11901123
references: Mol Phylogenet Evol. 2007 Oct;45(1):180-92
references: 17604184
references: Proc Biol Sci. 1998 Mar 22;265(1395):457-63
references: 9569664
references: Proc Biol Sci. 1992 Nov 23;250(1328):91-8
references: 1361987
references: Proc Biol Sci. 2003 Feb 7;270(1512):313-21
references: 12614582
references: Mol Phylogenet Evol. 2007 Jan;42(1):104-21
references: 16905338
references: Evol Bioinform Online. 2007 Feb 23;1:47-50
references: 19325852
references: J Mol Evol. 1994 Aug;39(2):174-90
references: 7932781
references: Heredity (Edinb). 2000 Jul;85 ( Pt 1):84-96
references: 10971694
references: Nature. 1983 Feb 17-23;301(5901):631-4
references: 6298629
references: Front Zool. 2007 Mar 07;4:8
references: 17343734
references: Heredity (Edinb). 2006 Oct;97(4):254-5
references: 16788705
references: Mol Ecol. 2012 Aug;21(16):3907-30
references: 22738314
references: Mol Ecol. 2004 Apr;13(4):729-44
references: 15012752
references: Evolution. 1999 Aug;53(4):1157-1164
references: 28565520
references: Proc Biol Sci. 2005 Aug 7;272(1572):1525-34
references: 16048766
references: Evolution. 2009 Mar;63(3):624-40
references: 19054050
references: Syst Biol. 2006 Oct;55(5):729-39
references: 17060195
references: BMC Evol Biol. 2011 Apr 01;11:86
references: 21457543
references: Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6491-5
references: 8022810
references: PLoS Biol. 2004 Oct;2(10):e354
references: 15486587
references: Genetics. 2004 Dec;168(4):2049-58
references: 15611174
references: Mol Ecol. 2001 Apr;10(4):899-910
references: 11348499
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Proc Biol Sci. 2007 Nov 22;274(1627):2881-9
references: 17785265
references: J Bacteriol. 1998 May;180(9):2373-8
references: 9573188
references: Genetics. 2003 May;164(1):5-12
references: 12750316
references: Ann Eugen. 1951 Mar;15(4):323-54
references: 24540312
references: Philos Trans R Soc Lond B Biol Sci. 2004 Apr 29;359(1444):669-79
references: 15253352
references: Evolution. 1995 Aug;49(4):718-726
references: 28565131
references: Genetics. 2005 Nov;171(3):1115-24
references: 16079227
references: Heredity (Edinb). 2010 Mar;104(3):318-26
references: 20087396
references: Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13486-91
references: 18757756
references: Genetica. 1993;92(1):55-60
references: 8163156
references: Mol Ecol. 2010 Sep;19(18):3881-93
references: 20701681
references: Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14812-7
references: 15465915
references: Bull Entomol Res. 2009 Aug;99(4):385-91
references: 19063762
references: Proc Biol Sci. 2000 Dec 22;267(1461):2475-82
references: 11197122
references: Mol Ecol. 2005 Apr;14(4):1133-50
references: 15773941
references: Ecol Evol. 2012 Dec;2(12):2974-88
references: 23301165
references: Insect Mol Biol. 1998 Feb;7(1):73-82
references: 9459431
references: PLoS One. 2011 Feb 14;6(2):e17134
references: 21423340
references: Proc Biol Sci. 1995 Jul 22;261(1360):55-63
references: 7644549
references: Mol Ecol. 2012 Sep;21(18):4422-36
references: 22891635
references: Mol Ecol. 2009 Nov;18(22):4541-50
references: 19821901