title: Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition.
abstract: The influence of diet composition--two substrates, wheat bran and sawdust--on isoform expression of digestive enzymes (cellulase, amylase and peptidase) in the midgut of Morimus funereus larvae was examined. Their impact on larval development was demonstrated by measuring the increase of larval weight during development and by analysis of digestive enzymes zymographic profiles, where the expression of cellulase isoforms from M. funereus larvae midgut has been examined for the first time in this study. Larvae reared on wheat bran had higher body weight between day 60 and day 100 than larvae reared on sawdust; however, both groups achieved similar body weight after day 110. Wheat bran as substrate induced different cellulase and amylase isoforms. Oak sawdust in substrate acted as inducer of peptidases. The highest cellulase activity and the greatest isoform variability were detected in the midgut extracts of larvae reared on wheat bran. From our results it can be assumed that M. funereus endocellulase, amylase and peptidase are secreted in the anterior midgut, and their concentration gradually decreases towards the hindgut.
authors: Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia. Electronic address: bdojnov@chem.bg.ac.rs.
authors: Biljana
authors: B
authors: Dojnov
authors: Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia.
authors: Ratko
authors: R
authors: Pavlović
authors: Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia.
authors: Nataša
authors: N
authors: Božić
authors: Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia.
authors: Aleksandra
authors: A
authors: Margetić
authors: Department of Insect Physiology and Biochemistry, Institute for Biological Research Siniša Stanković, University of Belgrade, Despota Stefana 142, Belgrade, Serbia.
authors: Vera
authors: V
authors: Nenadović
authors: Department of Insect Physiology and Biochemistry, Institute for Biological Research Siniša Stanković, University of Belgrade, Despota Stefana 142, Belgrade, Serbia.
authors: Jelisaveta
authors: J
authors: Ivanović
authors: Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia.
authors: Zoran
authors: Z
authors: Vujčić
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002480:Cellulase / Q000378:metabolism*; D001517:Coleoptera / Q000201:enzymology* / Q000254:growth & development; D004032:Diet; D004064:Digestive System / Q000201:enzymology; D005502:Food; D007527:Isoenzymes / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000254:growth & development; D010447:Peptide Hydrolases / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D000681:Amylases; D002480:Cellulase; D010447:Peptide Hydrolases
keywords: Amylase; Cellulase; Isoforms; Morimus funereus; Peptidase