title: Surface functionalization affects the zeta potential, coronal stability and membranolytic activity of polymeric nanoparticles.
abstract: Nano materials are commonly functionalized to boost their physicochemical properties. However, there is little known about the impact of these modifications on cellular systems. Herein, we synthesized eight types of polymeric nanoparticles (NPs) bearing different functional groups, and investigated their effects on interactions with cellular membranes. As models for particle membrane interactions, hemolysis assays using human red blood cells and culture with A549 cells were utilized. Under protein-free conditions, the NPs showed a wide distribution of zeta potentials (ζPs) which showed a good correlation with their hemolytic potential. However, in the presence of serum or lung lining fluid, the ζPs of all NPs coalesced towards a single common negative value and showed neither hemolytic activity nor cytotoxicity to A549 cells. Lipase and protease treatment of the coronated particles did not restore their reactivity. These result simply proves that particle functionalization influences the stability of the particle corona which, if intact, prevents hemolytic activity and membrane disrupture.
authors: ELEGI/Colt Laboratory, Centre for Inflammation Research, University of Edinburgh , Edinburgh , UK.
authors: Wan-Seob
authors: WS
authors: Cho
authors: Frank
authors: F
authors: Thielbeer
authors: Rodger
authors: R
authors: Duffin
authors: Emma M V
authors: EM
authors: Johansson
authors: Ian L
authors: IL
authors: Megson
authors: William
authors: W
authors: MacNee
authors: Mark
authors: M
authors: Bradley
authors: Ken
authors: K
authors: Donaldson
mesh_terms: D045744:Cell Line, Tumor; D055590:Electromagnetic Phenomena; D004912:Erythrocytes / Q000166:cytology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D016209:Interleukin-8; D011108:Polymers; D011506:Proteins; D013501:Surface-Active Agents