title: Isolation and characterization of mesenchymal stem cells obtained from reusable and disposable bone marrow collection filters.
abstract: OBJECTIVE
  To compare human mesenchymal stem cells obtained from reusable and disposable filters and to characterize them according to the criteria of the International Society of Cellular Therapy.
  
  
  METHODS
  Human mesenchymal stem cells were isolated from bone marrow collection reusable sets and compared with those obtained from disposable sets by washing the filters with cell culture media. The isolated cells were characterized according to the criteria of the International Society of Cellular Therapy using flow cytometry, differentiation in vitro, and cytochemistry techniques.
  
  
  RESULTS
  Samples were obtained from disposable (n=3) and from reusable collection sets (n=3). All samples obtained from bone marrow disposable sets successfully produced mesenchymal stem cells. All bone marrow derived mesenchymal stem cells were characterized and fulfilled the criteria established by International Society of Cellular Therapy.
  
  
  CONCLUSIONS
  This study showed that mesenchymal stem cells can also be obtained from reusable collection sets (which are still used in several centers around the world) to be employed in research as an alternative and ethical source.
authors: Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.
authors: Glaziane Cordeiro de
authors: GC
authors: Deus
authors: Marilia
authors: M
authors: Normanton
authors: Nelson
authors: N
authors: Hamerschlak
authors: Andrea Tiemi
authors: AT
authors: Kondo
authors: Andreza Alice Feitosa
authors: AA
authors: Ribeiro
authors: Anna Carla
authors: AC
authors: Goldberg
authors: Luciana Cavalheiro
authors: LC
authors: Marti
mesh_terms: D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation / Q000941:ethics / Q000379:methods*; D002478:Cells, Cultured; D004209:Disposable Equipment; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*