title: EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice.
abstract: Programmed cell death is essential for the development of multicellular organisms, yet pathways of plant programmed cell death and its regulation remain elusive. Here we report that ETERNAL TAPETUM 1, a basic helix-loop-helix transcription factor conserved in land plants, positively regulates programmed cell death in tapetal cells in rice anthers. eat1 exhibits delayed tapetal cell death and aborted pollen formation. ETERNAL TAPETUM 1 directly regulates the expression of OsAP25 and OsAP37, which encode aspartic proteases that induce programmed cell death in both yeast and plants. Expression and genetic analyses revealed that ETERNAL TAPETUM 1 acts downstream of TAPETUM DEGENERATION RETARDATION, another positive regulator of tapetal programmed cell death, and that ETERNAL TAPETUM 1 can also interact with the TAPETUM DEGENERATION RETARDATION protein. This study demonstrates that ETERNAL TAPETUM 1 promotes aspartic proteases triggering plant programmed cell death, and reveals a dynamic regulatory cascade in male reproductive development in rice.
authors: State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors: Ningning
authors: N
authors: Niu
authors: Wanqi
authors: W
authors: Liang
authors: Xijia
authors: X
authors: Yang
authors: Weilin
authors: W
authors: Jin
authors: Zoe A
authors: ZA
authors: Wilson
authors: Jianping
authors: J
authors: Hu
authors: Dabing
authors: D
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D057055:Aspartic Acid Proteases / Q000378:metabolism*; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016923:Cell Death; D032461:Chromosomes, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D012275:Oryza / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D010641:Phenotype; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D011485:Protein Binding; D012098:Reproduction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism
chemical_list: D057055:Aspartic Acid Proteases; D051792:Basic Helix-Loop-Helix Proteins; D010940:Plant Proteins
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