title: Improve carbon metabolic flux in Saccharomyces cerevisiae at high temperature by overexpressed TSL1 gene.
abstract: This study describes a novel strategy to improve the glycolysis flux of Saccharomyces cerevisiae at high temperature. The TSL1 gene-encoding regulatory subunit of the trehalose synthase complex was overexpressed in S. cerevisiae Z-06, which increased levels of trehalose synthase activity in extracts, enhanced stress tolerance and glucose consuming rate of the yeast cells. As a consequence, the final ethanol concentration of 185.5 g/L was obtained at 38 °C for 36 h (with productivity up to 5.2 g/L/h) in 7-L fermentor, and the ethanol productivity was 92.7 % higher than that of the parent strain. The results presented here provide a novel way to enhance the carbon metabolic flux at high temperature, which will be available for the purposes of producing other primary metabolites of commercial interest using S. cerevisiae as a host.
authors: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, 1800 Lihu Road, Wuxi, JiangSu Province, China.
authors: Xiang-Yang
authors: XY
authors: Ge
authors: Yan
authors: Y
authors: Xu
authors: Xiang
authors: X
authors: Chen
mesh_terms: D002244:Carbon / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D006358:Hot Temperature; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C532521:TSL1 protein, S cerevisiae; D000431:Ethanol; D002244:Carbon; D005947:Glucose
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