title: Analysis of Soluble protein complexes in Shigella flexneri reveals the influence of temperature on the amount of lipopolysaccharide.
abstract: Shigella flexneri, which is closely related to Escherichia coli, is the most common cause of the endemic form of shigellosis. In this study, 53 homomultimeric protein complexes and nine heteromultimeric protein complexes from S. flexneri 2a strain 2457T were separated and identified. Among these, three potential homomultimeric protein complexes had not been previously described. One complex, thought to be composed of 12 PhoN1 subunits, is a periplasmic protein with an unknown physiological role encoded on the virulence plasmid of S. flexneri. The abundance of the protein complexes was compared following growth at 37 or 30°C, and the abundance of three protein complexes (PyrB-PyrI, GlmS, and MglB) related to the synthesis of lipopolysaccharides (LPS) appeared to be temperature-dependent. Many studies have shown that LPS is essential to the virulence of S. flexneri. Here, we report the influence of temperature on the amount of LPS.
authors: Institute of Nuclear-Agricultural Science, Zhejiang University, Hangzhou 310029, China.
authors: Chang
authors: C
authors: Niu
authors: Na
authors: N
authors: Shang
authors: Xiang
authors: X
authors: Liao
authors: Erling
authors: E
authors: Feng
authors: Xiankai
authors: X
authors: Liu
authors: Dongshu
authors: D
authors: Wang
authors: Jie
authors: J
authors: Wang
authors: Peitang
authors: P
authors: Huang
authors: Yuejin
authors: Y
authors: Hua
authors: Li
authors: L
authors: Zhu
authors: Hengliang
authors: H
authors: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000235:genetics; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D012763:Shigella flexneri / Q000378:metabolism*; D012995:Solubility; D013696:Temperature; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D046912:Multiprotein Complexes; D020543:Proteome; D037521:Virulence Factors
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