title: Effects of different dietary lipid level on the growth, survival and immune-relating genes expression in Pacific white shrimp, Litopenaeus vannamei.
abstract: Five feeding trials based on the isonitrogenous and isoenergetic experimental diets containing 34% protein, 6%, 8%, 10%, 12% or 14% lipid respectively in the circulating water culture system for both 30 and 60 days were conducted to investigate the effect of the dietary lipid level on the growth and immunity in white shirmp, Litopenaeus vannamei adults. The body weight and specific growth rate of white shrimp in different treatments indicated that shrimps fed the diet of 12% lipid level for 30d and 10% lipid level for 60d had the best developmental status. The ability of respiratory burst in hemocytes was improved as the increase of dietary lipid level. The transcripts of LGBP and pPO were sensitive to the dietary lipid in hemocyte and hepatopancreas respectively. The activities of CAT, GPx and AKP were increased to a certain extend according to dietary lipid level. Qualification of MDA showed the lowest level in the sample subjected to 12% lipid level diet, indicating an optimal utilization of the dietary lipid and an efficient clearance of MDA in vivo. These results suggested that dietary lipid level of 10-12% significantly tunes the growth and enhance the immune abilities mainly via ROS pathway of L. vannamei.
authors: Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors: Sheng-peng
authors: SP
authors: Zhang
authors: Jin-feng
authors: JF
authors: Li
authors: Xiao-chun
authors: XC
authors: Wu
authors: Wei-jing
authors: WJ
authors: Zhong
authors: Jian-an
authors: JA
authors: Xian
authors: Shao-an
authors: SA
authors: Liao
authors: Yu-tao
authors: YT
authors: Miao
authors: An-li
authors: AL
authors: Wang
mesh_terms: D000821:Animal Feed; D000818:Animals; D017756:Aquaculture; D004032:Diet / Q000662:veterinary; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000166:cytology; D007113:Immunity, Innate; D008055:Lipids / Q000008:administration & dosage*; D008136:Longevity; D008315:Malondialdehyde / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016897:Respiratory Burst
chemical_list: D008055:Lipids; D008315:Malondialdehyde