title: Posttraining optogenetic manipulations of basolateral amygdala activity modulate consolidation of inhibitory avoidance memory in rats.
abstract: Memory consolidation studies, including those examining the role of the basolateral amygdala (BLA), have traditionally used techniques limited in their temporal and spatial precision. The development of optogenetics provides increased precision in the control of neuronal activity that can be used to address the temporal nature of the modulation of memory consolidation. The present experiments, therefore, investigated whether optogenetically stimulating and inhibiting BLA activity immediately after training on an inhibitory avoidance task enhances and impairs retention, respectively. The BLA of male Sprague-Dawley rats was transduced to express either ChR2(E123A) or archaerhodopsin-3 from the Halorubrum sodomense strain TP009 (ArchT). Immediately after inhibitory avoidance training, rats received optical stimulation or inhibition of the BLA, and 2 d later, rats' retention was tested. Stimulation of ChR2(E123A)-expressing neurons in the BLA using trains of 40-Hz light pulses enhanced retention, consistent with recording studies suggesting the importance of BLA activity at this frequency. Light pulses alone given to control rats had no effect on retention. Inhibition of ArchT-expressing neurons in the BLA for 15 min, but not 1 min, significantly impaired retention. Again, illumination alone given to control rats had no effect on retention, and BLA inhibition 3 h after training had no effect. These findings provide critical evidence of the importance of specific frequency patterns of activity in the BLA during consolidation and indicate that optogenetic manipulations can be used to alter activity after a learning event to investigate the processes underlying memory consolidation.
authors: Department of Psychology, University of Iowa, Iowa City, IA 52242, USA.
authors: Mary L
authors: ML
authors: Huff
authors: Rachel L
authors: RL
authors: Miller
authors: Karl
authors: K
authors: Deisseroth
authors: David E
authors: DE
authors: Moorman
authors: Ryan T
authors: RT
authors: LaLumiere
mesh_terms: D019943:Amino Acid Substitution; D000679:Amygdala / Q000502:physiology*; D000818:Animals; D019843:Archaeal Proteins / Q000378:metabolism; D001362:Avoidance Learning / Q000502:physiology*; D000075402:Channelrhodopsins; D008297:Male; D008568:Memory / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D062308:Optogenetics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012153:Retention, Psychology; D014161:Transduction, Genetic; D017494:Proton Pumps
chemical_list: D019843:Archaeal Proteins; D000075402:Channelrhodopsins; C055476:archaerhodopsin protein, Archaea; D017494:Proton Pumps
references: Biol Psychiatry. 2004 Mar 15;55(6):559-62
references: 15013823
references: Brain Res. 1995 Apr 3;676(1):183-8
references: 7796168
references: Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):14048-53
references: 9391150
references: Neuron. 2011 Jul 14;71(1):9-34
references: 21745635
references: Learn Mem. 2005 May-Jun;12(3):296-301
references: 15930508
references: Neurobiol Learn Mem. 1999 Mar;71(2):232-9
references: 10082642
references: J Comp Neurol. 1982 Jul 10;208(4):401-18
references: 7119168
references: Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4888-93
references: 19255436
references: Neurobiol Learn Mem. 1996 Nov;66(3):253-7
references: 8946419
references: Neurobiol Learn Mem. 2008 Oct;90(3):576-9
references: 18657626
references: J Neurosci. 2008 Jun 25;28(26):6642-51
references: 18579737
references: J Neurosci. 1999 Aug 1;19(15):6615-22
references: 10414989
references: J Neurosci. 2011 Sep 7;31(36):12748-58
references: 21900554
references: Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8477-81
references: 8078906
references: Eur J Neurosci. 2002 Oct;16(7):1223-6
references: 12405982
references: Annu Rev Neurosci. 2004;27:1-28
references: 15217324
references: Nature. 2011 Jun 29;475(7356):377-80
references: 21716290
references: Neurobiol Learn Mem. 1997 Mar;67(2):176-9
references: 9075247
references: Behav Neurosci. 2004 Feb;118(1):53-62
references: 14979782
references: Nat Neurosci. 2009 Jun;12(6):801-7
references: 19430471
references: Eur J Neurosci. 2004 Nov;20(10):2804-10
references: 15548223
references: Brain Stimul. 2011 Jan;4(1):1-6
references: 21255749
references: Nature. 2011 Mar 17;471(7338):358-62
references: 21389985
references: Braz J Med Biol Res. 1997 Feb;30(2):235-40
references: 9239310
references: Learn Mem. 2005 Sep-Oct;12(5):527-32
references: 16204205
references: J Neurosci. 2003 Jul 30;23(17):6754-8
references: 12890768
references: J Neurosci. 2007 Aug 29;27(35):9369-79
references: 17728450
references: Addict Biol. 2013 Jan;18(1):50-3
references: 22823160
references: Science. 2009 Apr 17;324(5925):354-9
references: 19299587
references: Behav Neurosci. 2012 Feb;126(1):204-8
references: 22141467
references: Science. 2000 Jan 14;287(5451):248-51
references: 10634773
references: Brain Res. 1975 Mar 28;86(3):509-13
references: 1116015
references: Nature. 2010 Jan 7;463(7277):98-102
references: 20054397
references: Acta Pharmacol Sin. 2000 Jun;21(6):481-93
references: 11360681
references: Brain Res. 1999 Jul 24;835(2):340-5
references: 10415392
references: Biol Psychiatry. 2012 Jun 15;71(12):1053-60
references: 22169096
references: Neurosci Biobehav Rev. 2012 Aug;36(7):1750-62
references: 22085800
references: Eur J Neurosci. 2003 Nov;18(9):2605-10
references: 14622162
references: Brain Res. 1994 Oct 24;661(1-2):97-103
references: 7834391
references: Nat Neurosci. 2010 Mar;13(3):387-92
references: 20081849