title: [Primers for (1,3)-β-glucan synthase gene amplification and partial characterization of the enzyme in Ganoderma lucidum].
abstract: BACKGROUND
  β-(1,3)(1,6)-D-glucan is fungal cell wall component that has demonstrated immunomodulatory and anti-cancer effects. The (1,3)-β-glucan synthase is one of the main enzymes involved in its biosynthesis.
  
  
  OBJECTIVE
  To design primers to partially amplify and characterize the (1,3)-β-glucan synthase gene and to determine them in Ganoderma lucidum (G. Lucidum) strain CP-132.
  
  
  METHODS
  The primers were designed on the basis of homologous genes in other fungi. Then, using the PCR technique, primers were tested using DNA extracted from the G. lucidum strain CP-382. Amplified sequences were compared with those from the GenBank.
  
  
  RESULTS
  Three primer pairs were designed; all of them produced amplicons of the expected size. The sequences obtained with primer pairs BGS2113UmF and BGS3097UmR, and BGS547UmF and BGS2113UmR matched with 2 sections of the (1,3)-β-glucan synthase gene. The deduced amino acid sequences showed high similarity with homologous genes from other fungi, particularly with those of the Agaricomycetes class.
  
  
  CONCLUSIONS
  The primer design to partially amplify the (1,3)-β-glucan synthase gene of G. lucidum using sequences from homologous genes was successful. These primers will allow to characterize this important enzyme in a wide group of fungi.
authors: Departamento de Bioingeniería, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, México, D. F., México.
authors: Jessica Valeria
authors: JV
authors: Guerrero-Torres
authors: Gerardo
authors: G
authors: Mata
authors: Daniel
authors: D
authors: Martínez-Carrera
authors: Claudio
authors: C
authors: Garibay-Orijel
authors: Roberto
authors: R
authors: Garibay-Orijel
mesh_terms: D000363:Agaricales / Q000201:enzymology / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D020940:Reishi / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D021901:DNA, Intergenic; D005656:Fungal Proteins; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase
keywords: (1,3)-β-glucan synthase; (1,3)-β-glucano sintasa; Cebadores; Ganoderma lucidum; Gen; Gene; Primers