title: Characterisation of Walker 256 breast carcinoma cells from two tumour cell banks as assessed using two models of secondary brain tumours.
abstract: BACKGROUND
  Metastatic brain tumours are a common end stage of breast cancer progression, with significant associated morbidity and high mortality. Walker 256 is a rat breast carcinoma cell line syngeneic to Wistar rats and commonly used to induce secondary brain tumours. Previously there has been the assumption that the same cancer cell line from different cell banks behave in a similar manner, although recent studies have suggested that cell lines may change their characteristics over time in vitro.
  
  
  METHODS
  In this study internal carotid artery injection and direct cerebral inoculation models of secondary brain tumours were used to determine the tumorigenicity of Walker 256 cells obtained from two cell banks, the American Type Culture Collection (ATCC), and the Cell Resource Centre for Medical Research at Tohoku University (CRCTU).
  
  
  RESULTS
  Tumour incidence and volume, plus immunoreactivity to albumin, IBA1 and GFAP, were used as indicators of tumorigenicity and tumour interaction with the host brain microenvironment. CRCTU Walker 256 cells showed greater incidence, larger tumour volume, pronounced blood-brain barrier disruption and prominent glial response when compared to ATCC cell line.
  
  
  CONCLUSIONS
  These findings indicate that immortalised cancer cell lines obtained from different cell banks may have diverse characteristics and behaviour in vivo.
authors: Adelaide Centre for Neuroscience Research, School of Medical Sciences, The University of Adelaide, Adelaide, South Australia, Australia. mounir.ghabriel@adelaide.edu.au.
authors: Kate M
authors: KM
authors: Lewis
authors: Elizabeth
authors: E
authors: Harford-Wright
authors: Robert
authors: R
authors: Vink
authors: Mounir N
authors: MN
authors: Ghabriel
references: J Neurooncol. 1992 Mar;12(3):197-204
references: 1583554
references: Clin Exp Metastasis. 1984 Apr-Jun;2(2):171-8
references: 6085723
references: Cancer Res. 1978 Sep;38(9):2962-7
references: 679204
references: Curr Oncol Rep. 2001 Nov;3(6):467-75
references: 11595114
references: Magn Reson Med. 2006 Nov;56(5):1001-10
references: 17029229
references: Cancer Res. 1979 Jul;39(7 Pt 1):2691-7
references: 445473
references: J Neurol Sci. 1995 Dec;134(1-2):26-32
references: 8747839
references: J Neurochem. 2009 May;109(3):694-705
references: 19187440
references: J Neurooncol. 1984;2(3):205-18
references: 6502195
references: J Neurooncol. 1984;2(3):195-204
references: 6502194
references: Methods Mol Biol. 2011;731:13-26
references: 21516394
references: Pharmacol Rev. 2005 Jun;57(2):173-85
references: 15914466
references: Arq Neuropsiquiatr. 2012 Jan;70(1):52-8
references: 22218474
references: Cancer Res. 1987 Dec 1;47(23):6384-7
references: 3677083
references: Methods Mol Biol. 2011;731:35-43
references: 21516396
references: Lung Cancer. 2004 Aug;45 Suppl 2:S253-7
references: 15552807
references: Cancer Lett. 1997 Feb 26;113(1-2):77-86
references: 9065805
references: Invasion Metastasis. 1984;4(4):189-97
references: 6533092
references: Proc Natl Acad Sci U S A. 1982 Nov;79(21):6574-8
references: 6959137
references: Pathol Oncol Res. 2003;9(3):147-58
references: 14530807
references: Cancer Res. 1983 Nov;43(11):5314-20
references: 6616467
references: Am J Pathol. 1992 Nov;141(5):1115-24
references: 1443046
references: Appl Immunohistochem Mol Morphol. 2012 Jan;20(1):82-90
references: 22157059
references: Cancer. 1997 May 15;79(10):2036-44
references: 9149033
references: J Neurol Neurosurg Psychiatry. 2008 Sep;79(9):1061-5
references: 18245140
references: J Neurooncol. 1986;4(1):5-16
references: 3746386
references: Cancer Lett. 2007 Jan 8;245(1-2):350-2
references: 16504380
references: Cancer Res. 1978 Oct;38(10):3174-81
references: 210930
references: Cancer Res. 1981 Apr;41(4):1368-72
references: 7214323
references: Nat Rev Cancer. 2010 Jun;10(6):441-8
references: 20448633
references: Cancer Res. 1990 Oct 1;50(19):6130-8
references: 1698118
references: Clin Exp Metastasis. 2013 Jan;30(1):1-12
references: 22610781
references: Int J Cancer. 2009 May 15;124(10):2488-94
references: 19142969
references: Neurosci Lett. 2010 Aug 30;481(1):26-9
references: 20600611
references: Ann Oncol. 2008 Sep;19(9):1561-5
references: 18534964
references: Cancer Res. 1978 Sep;38(9):2651-60
references: 354778
references: J Neurochem. 2012 Jul;122(1):208-20
references: 22519988
references: Recent Pat CNS Drug Discov. 2011 Jan;6(1):31-40
references: 21073431
references: In Vitro Cell Dev Biol Anim. 2004 Jul-Aug;40(7):211-5
references: 15638703
references: J Neurosurg. 1993 Jun;78(6):959-65
references: 8487079
references: Cancer Res. 1982 Jul;42(7):2770-8
references: 7083167
references: Cancer Res. 1992 Jan 1;52(1):138-43
references: 1727374
references: Ann Neurol. 1986 Jan;19(1):50-9
references: 3080944
references: Life Sci. 2007 Feb 13;80(10):950-8
references: 17169379
references: J Neurooncol. 1997 Oct;35(1):81-9
references: 9266444
references: J Neuroimmunol. 2012 Sep 15;250(1-2):59-65
references: 22722013
references: Genes Chromosomes Cancer. 2000 Mar;27(3):252-63
references: 10679914
references: J Neurosurg. 1983 Aug;59(2):304-10
references: 6864299
references: In Vitro Cell Dev Biol Anim. 2010 Oct;46(9):727-32
references: 20614197