title: A simple point-of-care microfluidic immunomagnetic fluorescence assay for pathogens.
abstract: In this work, we reported a simple rapid and point-of-care magnetic immunofluorescence assay for avian influenza virus (AIV) and developed a portable experimental setup equipped with an optical fiber spectrometer and a microfluidic device. We achieved the integration of immunomagnetic target capture, concentration, and fluorescence detection in the microfluidic chip. By optimizing flow rate and incubation time, we could get a limit of detection low up to 3.7 × 10(4) copy/μL with a sample consumption of 2 μL and a total assay time of less than 55 min. This approach had proved to possess high portability, fast analysis, high specificity, high precision, and reproducibility with an intra-assay variability of 2.87% and an interassay variability of 4.36%. As a whole, this microfluidic system may provide a powerful platform for the rapid detection of AIV and may be extended for detection of other viral pathogens; in addition, this portable experimental setup enables the development of point-of-care diagnostic systems while retaining adequate sensitivity.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
authors: Rui-Qiao
authors: RQ
authors: Zhang
authors: Shu-Lin
authors: SL
authors: Liu
authors: Wei
authors: W
authors: Zhao
authors: Wan-Po
authors: WP
authors: Zhang
authors: Xu
authors: X
authors: Yu
authors: Yong
authors: Y
authors: Li
authors: An-Jun
authors: AJ
authors: Li
authors: Dai-Wen
authors: DW
authors: Pang
authors: Zhi-Ling
authors: ZL
authors: Zhang
mesh_terms: D000818:Animals; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification; D009980:Influenza A virus / Q000302:isolation & purification*; D057230:Limit of Detection; D060526:Magnetic Fields; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008962:Models, Theoretical; D055100:Optical Fibers; D019095:Point-of-Care Systems; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013997:Time Factors