title: Protein kinase/phosphatase function correlates with gliding motility in Mycoplasma pneumoniae.
abstract: Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-deficient phenotypes. Transposon insertions in the only annotated Ser/Thr protein kinase gene (prkC; MPN248) and its cognate phosphatase gene (prpC; MPN247) in M. pneumoniae resulted in significant and contrasting effects on gliding frequencies. prkC mutant cells glided at approximately half the frequency of wild-type cells, while prpC mutant cells glided more than twice as frequently as wild-type cells. Phosphoprotein staining confirmed the association between phosphorylation of the cytoskeletal proteins HMW1 and HMW2 and membrane protein P1 and the gliding phenotype. When the prpC mutant was complemented by transposon delivery of a wild-type copy of the prpC allele, gliding frequencies and phosphorylation levels returned to the wild-type standard. Surprisingly, delivery of the recombinant wild-type prkC allele dramatically increased gliding frequency to a level approximately 3-fold greater than that of wild-type in the prkC mutant. Collectively, these data suggest that PrkC and PrpC work in opposition in M. pneumoniae to influence gliding frequency.
authors: Department of Microbiology, University of Georgia, Athens, GA, USA.
authors: Clinton A
authors: CA
authors: Page
authors: Duncan C
authors: DC
authors: Krause
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D009068:Movement / Q000502:physiology; D016296:Mutagenesis; D009154:Mutation; D009177:Mycoplasma pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D047428:Protein Kinase Inhibitors; D011994:Recombinant Proteins; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D019311:Staurosporine
references: J Bacteriol. 1997 Apr;179(8):2668-77
references: 9098066
references: Mol Cell Proteomics. 2010 Jun;9(6):1228-42
references: 20097688
references: Cell. 2008 Oct 31;135(3):486-96
references: 18984160
references: J Bacteriol. 1999 Jul;181(14):4404-10
references: 10400600
references: Microbiology (Reading). 2001 Apr;147(Pt 4):1045-1057
references: 11283300
references: Infect Immun. 1982 Mar;35(3):809-17
references: 6802761
references: J Bacteriol. 2005 Mar;187(5):1875-7
references: 15716461
references: J Bacteriol. 2012 Jun;194(11):3000-7
references: 22544269
references: Mol Microbiol. 1994 Jul;13(2):337-48
references: 7984111
references: Clin Microbiol Rev. 2004 Oct;17(4):697-728, table of contents
references: 15489344
references: J Bacteriol. 2006 Sep;188(17):6335-45
references: 16923901
references: Infect Immun. 1995 Sep;63(9):3479-83
references: 7642280
references: J Bacteriol. 2005 May;187(10):3502-10
references: 15866938
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Microbiol Mol Biol Rev. 1999 Sep;63(3):621-41
references: 10477310
references: J Bacteriol. 2004 Mar;186(5):1537-45
references: 14973017
references: J Bacteriol. 2005 Aug;187(16):5578-84
references: 16077102
references: J Bacteriol. 1992 Jul;174(13):4265-74
references: 1624421
references: Nucleic Acids Res. 1996 Nov 15;24(22):4420-49
references: 8948633
references: J Bacteriol. 1995 Feb;177(3):843-6
references: 7836325
references: Mol Microbiol. 1991 Feb;5(2):299-306
references: 1904124
references: J Bacteriol. 1998 Mar;180(5):1270-6
references: 9495768
references: J Bacteriol. 1995 Aug;177(15):4571-4
references: 7635846
references: Microbiol Mol Biol Rev. 2011 Mar;75(1):192-212
references: 21372323
references: Gene. 1996 May 24;171(1):79-82
references: 8675035
references: J Bacteriol. 2004 Oct;186(20):6944-55
references: 15466048
references: Front Biosci. 2002 May 01;7:d1338-46
references: 11991833
references: Infect Immun. 2010 Jan;78(1):184-92
references: 19858294
references: J Biol Chem. 2006 Sep 8;281(36):26253-9
references: 16857667
references: J Bacteriol. 2009 Nov;191(21):6741-8
references: 19717588
references: Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16478-83
references: 17062751
references: J Bacteriol. 1999 Feb;181(4):1079-87
references: 9973332
references: Science. 1977 Mar 4;195(4281):892-4
references: 841314
references: J Exp Med. 1983 Feb 1;157(2):502-14
references: 6401796
references: Mol Syst Biol. 2012 Feb 28;8:571
references: 22373819
references: Mol Microbiol. 2009 Mar;71(5):1296-307
references: 19183275
references: Nucleic Acids Res. 2000 Sep 1;28(17):3278-88
references: 10954595
references: Trends Pharmacol Sci. 1989 Jun;10(6):218-20
references: 2672462
references: Infect Immun. 2007 Jan;75(1):518-22
references: 17043103
references: Plasmid. 1993 Sep;30(2):170-5
references: 8234492
references: J Bacteriol. 2002 Jun;184(11):3061-8
references: 12003948
references: Microbiology (Reading). 2009 Mar;155(Pt 3):932-943
references: 19246764
references: J Bacteriol. 1969 Nov;100(2):1037-43
references: 5359607
references: Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4
references: 388439
references: J Bacteriol. 1982 Sep;151(3):1514-22
references: 6809731
references: J Bacteriol. 1994 Dec;176(24):7499-505
references: 8002573