title: [Standardizing a central nervous system tumor DNA biobank].
abstract: BACKGROUND
  Brain tumors are one of the leading cancers worldwide; in the National Institute of Neurology and Neurosurgery (INNN) these tumors are the leading cause of morbitity and mortality.
  
  
  OBJECTIVE
  Standardize biopsies, colletion, processing and storage biologic material of molecular studies.
  
  
  METHODS
  with a previously signed surgical consent, a tumor and blood biopsy was done to 134 patients. Their DNA was extracted and a database was filled considering technical, ethical and legal aspects. In order to have optimal biologic material the procedure was standardized between the surgical and research laboratory teams.
  
  
  RESULTS
  The biopsy, transportation, processing and storage were standardized. 134 patients were included (67 male and 67 female) with an average age of 46.28 years (range 15-81). The most frequently biopsied tumor was the meningioma (42%). The integrity of the obtained material was determined by agarose gel electrophoretic analysis.
  
  
  CONCLUSIONS
  the INNN biobank has a standardized system that biopsies, processes and stores optimum quality biologic material that will be the basis of future molecular studies.
authors: Departamento de Neurocirugía, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, La Fama, Tlalpan, México, D.F., Mexico. diegomendezrosito@gmail.com
authors: Diego
authors: D
authors: Méndez-Rosito
authors: Edgar
authors: E
authors: Rangel-López
authors: María Lucinda
authors: ML
authors: Aguirre-Cruz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018070:Biological Specimen Banks / Q000458:organization & administration / Q000592:standards*; D001706:Biopsy / Q000592:standards; D002490:Central Nervous System / Q000737:chemistry / Q000473:pathology; D016543:Central Nervous System Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology* / Q000556:secondary; D003390:Cranial Nerve Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D016208:Databases, Factual; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D008579:Meningioma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D008800:Mexico; D008875:Middle Aged; D018302:Neoplasms, Neuroepithelial / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D010524:Peripheral Nervous System Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D011309:Preservation, Biological / Q000379:methods / Q000592:standards; D011785:Quality Assurance, Health Care; D013048:Specimen Handling / Q000592:standards; D013127:Spinal Nerves / Q000737:chemistry / Q000473:pathology; D014186:Transportation / Q000592:standards; D055815:Young Adult
chemical_list: D004273:DNA, Neoplasm