title: Conjugated linoleic acid reduces adiposity and increases markers of browning and inflammation in white adipose tissue of mice.
abstract: The objective of this study was to examine the mechanism by which conjugated linoleic acid (CLA) reduces body fat. Young male mice were fed three combinations of fatty acids at three doses (0.06%, 0.2%, and 0.6%, w/w) incorporated into AIN76 diets for 7 weeks. The types of fatty acids were linoleic acid (control), an equal mixture of trans-10, cis-12 (10,12) CLA plus linoleic acid, and an equal isomer mixture of 10,12 plus cis-9, trans-11 (9,11) CLA. Mice receiving the 0.2% and 0.6% dose of 10,12 CLA plus linoleic acid or the CLA isomer mixture had decreased white adipose tissue (WAT) and brown adipose tissue (BAT) mass and increased incorporation of CLA isomers in epididymal WAT and liver. Notably, in mice receiving 0.2% of both CLA treatments, the mRNA levels of genes associated with browning, including uncoupling protein 1 (UCP1), UCP1 protein levels, and cytochrome c oxidase activity, were increased in epididymal WAT. CLA-induced browning in WAT was accompanied by increases in mRNA levels of markers of inflammation. Muscle cytochrome c oxidase activity and BAT UCP1 protein levels were not affected by CLA treatment. These data suggest a linkage between decreased adiposity, browning in WAT, and low-grade inflammation due to consumption of 10,12 CLA.
authors: Department of Nutrition, University of North Carolina at Greensboro, Greensboro, NC, USA.
authors: Wan
authors: W
authors: Shen
authors: Chia-Chi
authors: CC
authors: Chuang
authors: Kristina
authors: K
authors: Martinez
authors: Tanya
authors: T
authors: Reid
authors: J Mark
authors: JM
authors: Brown
authors: Lin
authors: L
authors: Xi
authors: Lindsay
authors: L
authors: Hixson
authors: Robin
authors: R
authors: Hopkins
authors: Joseph
authors: J
authors: Starnes
authors: Michael
authors: M
authors: McIntosh
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects* / Q000378:metabolism*; D050154:Adiposity / Q000187:drug effects*; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D015151:Immunoblotting; D007249:Inflammation / Q000378:metabolism*; D019787:Linoleic Acid / Q000494:pharmacology; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D014280:Triglycerides; D019787:Linoleic Acid
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