title: A KS-type dehydrin and its related domains reduce Cu-promoted radical generation and the histidine residues contribute to the radical-reducing activities.
abstract: Dehydrin is a plant disordered protein whose functions are not yet totally understood. Here it is reported that a KS-type dehydrin can reduce the formation of reactive oxygen species (ROS) from Cu. AtHIRD11, which is the Arabidopsis KS-type dehydrin, inhibited generation of hydrogen peroxide and hydroxyl radicals in the Cu-ascorbate system. The radical-reducing activity of AtHIRD11 was stronger than those of radical-silencing peptides such as glutathione and serum albumin. The addition of Cu(2+) reduced the disordered state, decreased the trypsin susceptibility, and promoted the self-association of AtHIRD11. Domain analyses indicated that the five domains containing histidine showed ROS-reducing activities. Histidine/alanine substitutions indicated that histidine is a crucial residue for reducing ROS generation. Using the 27 peptides which are related to the KnS-type dehydrins of 14 plant species, it was found that the strengths of ROS-reducing activities can be determined by two factors, namely the histidine contents and the length of the peptides. The degree of ROS-reducing activities of a dehydrin can be predicted using these indices.
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. amhara@ipc.shizuoka.ac.jp
authors: Masakazu
authors: M
authors: Hara
authors: Mitsuru
authors: M
authors: Kondo
authors: Takanari
authors: T
authors: Kato
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D060326:Chemistry Techniques, Synthetic; D003300:Copper / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; C070556:dehydrin proteins, plant; D017665:Hydroxyl Radical; D006639:Histidine; D003300:Copper; D006861:Hydrogen Peroxide
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