title: Topically delivered adipose derived stem cells show an activated-fibroblast phenotype and enhance granulation tissue formation in skin wounds.
abstract: Multipotent mesenchymal stem cells (MSCs) are found in various tissues and can proliferate extensively in vitro. MSCs have been used in preclinical animal studies and clinical trials in many fields. Adipose derived stem cells (ASCs) have several advantages compared to other MSCs for use in cell-based treatments because they are easy to isolate with relative abundance. However, quantitative approaches for wound repair using ASCs have been limited because of lack of animal models which allow for quantification. Here, we addressed the effect of topically delivered ASCs in wound repair by quantitative analysis using the rabbit ear model. We characterized rabbit ASCs, and analyzed their multipotency in comparison to bone marrow derived-MSCs (BM-MSCs) and dermal fibroblasts (DFs) in vitro. Topically delivered ASCs increased granulation tissue formation in wounds when compared to saline controls, whereas BM-MSCs or DFs did not. These studies suggest that ASCs and BM-MSCs are not identical, though they have similar surface markers. We found that topically delivered ASCs are engrafted and proliferate in the wounds. We showed that transplanted ASCs exhibited activated fibroblast phenotype, increased endothelial cell recruitment, and enhanced macrophage recruitment in vivo.
authors: Department of Surgery/Division of Plastic and Reconstructive Surgery, Laboratory for Wound Repair and Regenerative Medicine, Feinberg School of Medicine,Northwestern University, Chicago, Illinois, United States of America. seok-hong@northwestern.edu
authors: Seok Jong
authors: SJ
authors: Hong
authors: Sheng-Xian
authors: SX
authors: Jia
authors: Ping
authors: P
authors: Xie
authors: Wei
authors: W
authors: Xu
authors: Kai P
authors: KP
authors: Leung
authors: Thomas A
authors: TA
authors: Mustoe
authors: Robert D
authors: RD
authors: Galiano
mesh_terms: D000199:Actins / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D024061:Collagen Type III / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006097:Granulation Tissue / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype; D011817:Rabbits; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D014945:Wound Healing
chemical_list: D000199:Actins; D024061:Collagen Type III
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