title: Comparison of cytokine gene polymorphisms among Greek patients with invasive meningococcal disease or viral meningitis.
abstract: High levels of pro-inflammatory cytokines are implicated in the severity of invasive meningococcal disease (IMD) and viral meningitis (VM). This study compared single-nucleotide polymorphisms (SNPs) in pro- and anti-inflammatory cytokine genes among patients with VM or IMD. Patient DNA samples were prepared by the National Meningitis Reference Laboratory in Athens: n=98 for IMD and n=53 for VM. The results for both patient groups were compared with data published for healthy Greek control data. Real-time PCR was used to assess the interleukin (IL) gene SNPs IL6 G-174C, IL1B C-511T, IL1RN T+2018C, IL10 G-1082A and IL8 A-251T and the tumour necrosis factor α (TNF-α) SNP TNFA G-308A. Differences were compared by Fisher's exact test. The genotype for high IL-6 responses was predominant among IMD (51%, P=0.0008) and VM (74.5%, P<0.0001) patients compared with the controls (31%). The genotype associated with high TNF-α responses was 5% among controls and lower for IMD (1.1%, P=0.0014) and VM (0%, P=0.052). There was no difference for IL-8 SNPs between controls and IMD (P=0.162), but the difference was significant for VM (P=0.0025). IL-6 (P=0.024) and IL-8 (P=0.00004) SNPs differed between IMD and VM. Reports on associations between IL-8 SNPs and cytokine responses differ. Because of its role in neutrophil attraction, differences in frequencies of the IL-8 SNP for IMD and VM require further investigation.
authors: University of Newcastle, Newcastle, NSW, Australia.
authors: Callum J
authors: CJ
authors: Titmarsh
authors: University of Newcastle, Newcastle, NSW, Australia.
authors: Sophia M
authors: SM
authors: Moscovis
authors: Hunter Medical Research Institute, University of Newcastle, Newcastle, NSW, Australia.|University of Newcastle, Newcastle, NSW, Australia.
authors: Sharron
authors: S
authors: Hall
authors: National Meningitis Reference Laboratory, National School of Public Health, Athens, Greece.
authors: Georgina
authors: G
authors: Tzanakaki
authors: National Meningitis Reference Laboratory, National School of Public Health, Athens, Greece.
authors: Konstantinos
authors: K
authors: Kesanopoulos
authors: National Meningitis Reference Laboratory, National School of Public Health, Athens, Greece.
authors: Athanasia
authors: A
authors: Xirogianni
authors: Hunter Area Pathology Service Genetics, University of Newcastle, Newcastle, NSW, Australia.|Hunter Medical Research Institute, University of Newcastle, Newcastle, NSW, Australia.|University of Newcastle, Newcastle, NSW, Australia.
authors: Rodney J
authors: RJ
authors: Scott
authors: Hunter Medical Research Institute, University of Newcastle, Newcastle, NSW, Australia.|University of Newcastle, Newcastle, NSW, Australia.
authors: C Caroline
authors: CC
authors: Blackwell
mesh_terms: D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006115:Greece / Q000453:epidemiology; D006801:Humans; D008585:Meningitis, Meningococcal / Q000453:epidemiology / Q000235:genetics*; D008587:Meningitis, Viral / Q000453:epidemiology / Q000235:genetics*; D009345:Neisseria meningitidis; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D018805:Sepsis / Q000235:genetics / Q000382:microbiology
chemical_list: D016207:Cytokines