title: Intracellular position of G2/M-phase nuclei in neoplastic and non-neoplastic pseudostratified glands suggests the occurrence of interkinetic nuclear migration.
abstract: It has been established that nuclear pseudostratification of the neural epithelium in vertebral embryos is caused by interkinetic nuclear migration, a cell cycle-dependent regulation of nuclear movement, during which the G2/M-phase nuclei move apically before returning basally in the G1/S phase. Here we demonstrate the cell cycle-related nuclear location characteristic of interkinetic nuclear migration in human neoplastic and non-neoplastic pseudostratified glands. Immunohistochemical analysis with phosphohistone H3 (a G2/M-phase marker) and Ki67 was performed on fetal tissues, proliferative-phase endometrium (5 cases), and colonic adenomas (12 cases). In all cases, G2/M nuclei were significantly located apically, whereas Ki67-positive nuclei were widely distributed along the basal-apical axis. In the proliferating zone of the normal colon mucosa, elongated nuclei in the G2/M phase were occasionally found on the apical side of the cells. These results suggest that the interkinetic nuclear migration occurs in association with cell proliferation in both neoplastic and non-neoplastic glands.
authors: Chiba University Graduate School of Medicine, 1-8-1, Inohana, Chuo-ku, Chiba, 260-8670, Japan, tkishi@faculty.chiba-u.jp.
authors: Takashi
authors: T
authors: Kishimoto
authors: Kazunori
authors: K
authors: Fugo
authors: Takako
authors: T
authors: Kiyokawa
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000236:Adenoma / Q000473:pathology*; D002467:Cell Nucleus / Q000502:physiology*; D049109:Cell Proliferation; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D004717:Endometrium / Q000166:cytology; D004848:Epithelium / Q000473:pathology; D005260:Female; D005333:Fetus / Q000166:cytology; D059565:G2 Phase Cell Cycle Checkpoints; D006657:Histones / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones
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