title: Analysis of transplant rejection in rabbits aponeurosis.
abstract: OBJECTIVE
  To study the feasibility of heterologous aponeurosis to close the abdominal wall of rabbits emphasizing the rejection process.
  
  
  METHODS
  This project was approved by the Animals Care Committee of the Faculdade de Ciências Médicas da Santa Casa de São Paulo, and it was carried out at the Experimental Surgery and Technical Unit. Four red female rabbits exchanged abdominal wall aponeurosis with other four white male animals. Two rabbits just had it removed and replaced to be the control group for the healing process. Animals were evaluated once a day, and after 7 days they were sacrificed. Immunohistochemical analysis with CD20 and CD79 was done.
  
  
  RESULTS
  The animals did not have cellulitis, abscess, hematoma, wound dehiscence or herniation. The graft site showed intense hyperemia. The histological analysis showed an inflammatory process with maturing myofibroblasts and collagen ranging from incipient to moderate. The number of vessels was reduced and the inflammatory cells were most plasma cells and macrophages. There were no morphological signs of acute rejection with aggressive vascular endothelial damage. The adjacent muscle showed signs of degeneration with inflammatory centralization of nuclei and cytoplasmic condensation. The immunohistochemical analysis (CD20 and CD79) showed that the inflammatory process was not mediated by lymphocytes. Mann-Whitney nonparametric test showed that the hypothesis of equality (p>0.05) should not be discarded.
  
  
  CONCLUSIONS
  There were no postoperative complications (fistulas, dehiscence etc.) and the histological analysis showed nonspecific inflammatory process. The immunohistochemical analysis showed that the inflammatory process was not due to a possible rejection.
authors: Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
authors: Andre Ibrahim
authors: AI
authors: David
authors: Eduardo Sauerbronn
authors: ES
authors: Gouvea
authors: Felipe Ribeiro
authors: FR
authors: Batista
authors: Paulo Henrique Fogaça de
authors: PH
authors: Barros
authors: Dino
authors: D
authors: Martini Filho
authors: Victor
authors: V
authors: Arias
authors: Paulo Roberto
authors: PR
authors: Corsi
authors: Luiz Arnaldo
authors: LA
authors: Szutan
mesh_terms: D034861:Abdominal Wall / Q000473:pathology / Q000601:surgery*; D000818:Animals; D018951:Antigens, CD20 / Q000032:analysis; D051925:CD79 Antigens / Q000032:analysis; D004730:Endothelium, Vascular; D005205:Fascia / Q000473:pathology / Q000637:transplantation*; D005240:Feasibility Studies; D005260:Female; D006084:Graft Rejection; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D013710:Tendons / Q000637:transplantation*; D014945:Wound Healing
chemical_list: D018951:Antigens, CD20; D051925:CD79 Antigens