title: Extraction, purification and preliminary characterization of polysaccharides from Kadsura marmorata fruits.
abstract: In this study, response surface methodology and central composite design based on single-factor experiments were applied to the optimization of the extraction parameters (extraction time, extraction temperature, and solid-liquid ratio) of polysaccharides from Kadsura marmorata fruits. The extraction temperature and solid-liquid ratio affected the extraction yield significantly. The optimal conditions included a solid-liquid ratio of 1:27 (mass/volume), an extraction time of 3h and an extraction temperature of 98 °C. Under these conditions, the maximal yield of crude Kadsura polysaccharide (KPS) was 2.7735±0.068%, which agreed with model predictions. Five major fractions, KPS I, KPS II, KPS III, KPS IV and KPS V, were obtained by diethylaminoethyl (DEAE) cellulose-52 column chromatography. The main fraction, KPS III-1, was isolated from KPS III by Sephadex G-100 column chromatography. Preliminary characterization of KPS III-1 by gas chromatography indicated that it was composed mainly of xylose and galacturonic acid.
authors: Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, No. 48 People's Street, Jinzhou, Liaoning 121001, PR China. wanghj0312@yahoo.com.cn
authors: Hongjun
authors: H
authors: Wang
authors: Hong
authors: H
authors: Jiang
authors: Shude
authors: S
authors: Wang
authors: Xinguo
authors: X
authors: Li
authors: Dan
authors: D
authors: Yao
authors: Jingjing
authors: J
authors: Dong
authors: Tiezhong
authors: T
authors: Zhou
authors: Yingzi
authors: Y
authors: Liu
authors: Shenyang
authors: S
authors: Gao
authors: Li
authors: L
authors: Li
authors: Xuming
authors: X
authors: Deng
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002849:Chromatography, Gas; D005638:Fruit / Q000737:chemistry*; D032135:Kadsura / Q000737:chemistry*; D015233:Models, Statistical; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors
chemical_list: D011134:Polysaccharides