title: High-throughput hyperdimensional vertebrate phenotyping.
abstract: Most gene mutations and biologically active molecules cause complex responses in animals that cannot be predicted by cell culture models. Yet animal studies remain too slow and their analyses are often limited to only a few readouts. Here we demonstrate high-throughput optical projection tomography with micrometre resolution and hyperdimensional screening of entire vertebrates in tens of seconds using a simple fluidic system. Hundreds of independent morphological features and complex phenotypes are automatically captured in three dimensions with unprecedented speed and detail in semitransparent zebrafish larvae. By clustering quantitative phenotypic signatures, we can detect and classify even subtle alterations in many biological processes simultaneously. We term our approach hyperdimensional in vivo phenotyping. To illustrate the power of hyperdimensional in vivo phenotyping, we have analysed the effects of several classes of teratogens on cartilage formation using 200 independent morphological measurements, and identified similarities and differences that correlate well with their known mechanisms of actions in mammals.
authors: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors: Carlos
authors: C
authors: Pardo-Martin
authors: Amin
authors: A
authors: Allalou
authors: Jaime
authors: J
authors: Medina
authors: Peter M
authors: PM
authors: Eimon
authors: Carolina
authors: C
authors: Wählby
authors: Mehmet
authors: M
authors: Fatih Yanik
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000002:abnormalities / Q000187:drug effects / Q000473:pathology; D019465:Craniofacial Abnormalities / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007814:Larva / Q000187:drug effects; D010641:Phenotype; D013723:Teratogens / Q000633:toxicity; D014054:Tomography / Q000379:methods*; D014714:Vertebrates / Q000033:anatomy & histology* / Q000254:growth & development; D015027:Zebrafish / Q000033:anatomy & histology / Q000254:growth & development
chemical_list: D013723:Teratogens
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