title: Crystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases.
abstract: Fructosyl peptide oxidase (FPOX) catalyses the oxidation of α-glycated dipeptides such as N(α)-(1-deoxy-D-fructos-1-yl)-L-valyl-L-histidine (Fru-ValHis) and is used in the diagnosis of diabetes mellitus. Here, two thermostable mutants of FPOX, CFP-T7 and EFP-T5M, were crystallized by the sitting-drop vapour-diffusion method. The crystal of CFP-T7 belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 110.09, c = 220.48 Å, and that of EFP-T5M belonged to the monoclinic space group P2(1), with unit-cell parameters a = 43.00, b = 230.05, c = 47.27 Å, β = 116.99°. The crystals of CFP-T7 and EFP-T5M diffracted to 1.8 and 1.6 Å resolution, respectively.
authors: Research and Development Division, Kikkoman Corporation, 399 Noda, Noda-shi, Chiba 278-0037, Japan. aichiyanagi@mail.kikkoman.co.jp
authors: Atsushi
authors: A
authors: Ichiyanagi
authors: Kozo
authors: K
authors: Hirokawa
authors: Keiko
authors: K
authors: Gomi
authors: Toru
authors: T
authors: Nakatsu
authors: Hiroaki
authors: H
authors: Kato
authors: Naoki
authors: N
authors: Kajiyama
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D055324:Eupenicillium / Q000201:enzymology*; D032641:Eurotiales / Q000201:enzymology*
chemical_list: D000594:Amino Acid Oxidoreductases; C520096:fructosyl-peptide oxidase
keywords: Coniochaeta sp.; Eupenicillium terrenum; diagnosis of diabetes; fructosyl peptide oxidase; haemoglobin A1c
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