title: Optofluidic cell manipulation for a biological microbeam.
abstract: This paper describes the fabrication and integration of light-induced dielectrophoresis for cellular manipulation in biological microbeams. An optoelectronic tweezers (OET) cellular manipulation platform was designed, fabricated, and tested at Columbia University's Radiological Research Accelerator Facility (RARAF). The platform involves a light induced dielectrophoretic surface and a microfluidic chamber with channels for easy input and output of cells. The electrical conductivity of the particle-laden medium was optimized to maximize the dielectrophoretic force. To experimentally validate the operation of the OET device, we demonstrate UV-microspot irradiation of cells containing green fluorescent protein (GFP) tagged DNA single-strand break repair protein, targeted in suspension. We demonstrate the optofluidic control of single cells and groups of cells before, during, and after irradiation. The integration of optofluidic cellular manipulation into a biological microbeam enhances the facility's ability to handle non-adherent cells such as lymphocytes. To the best of our knowledge, this is the first time that OET cell handling is successfully implemented in a biological microbeam.
authors: Department of Mechanical Engineering, Columbia University, New York, New York 10027, USA. mg2705@columbia.edu
authors: Michael
authors: M
authors: Grad
authors: Alan W
authors: AW
authors: Bigelow
authors: Guy
authors: G
authors: Garty
authors: Daniel
authors: D
authors: Attinger
authors: David J
authors: DJ
authors: Brenner
mesh_terms: D045744:Cell Line, Tumor; D004249:DNA Damage; D004586:Electrophoresis; D004867:Equipment Design; D006801:Humans; D007834:Lasers; D008863:Microspheres; D055616:Microtechnology / Q000295:instrumentation*; D052898:Optical Tweezers; D017785:Photons; D012984:Software
references: J Biomed Opt. 2012 Jan;17(1):015001
references: 22352645
references: Science. 1983 Apr 29;220(4596):524-7
references: 6836297
references: Nature. 2005 Jul 21;436(7049):370-2
references: 16034413
references: J Colloid Interface Sci. 2005 May 1;285(1):419-28
references: 15797441
references: J Biophotonics. 2008 Mar;1(1):24-35
references: 19343632
references: J Microelectromech Syst. 2008 Apr;17(2):342-350
references: 19079767
references: Mutat Res. 2011 Jun 3;711(1-2):87-99
references: 21126526
references: J Instrum. 2012 Dec;7:
references: 24058378
references: AIP Conf Proc. 2010 Aug 8;1336:351-355
references: 23420504
references: Appl Phys Lett. 2009 Sep 14;95(11):113104
references: 19884988
references: Anal Chem. 2011 Jun 15;83(12):4863-70
references: 21542583
references: J Radiat Res. 2009 Mar;50 Suppl A:A21-8
references: 19346682
references: Electrophoresis. 2008 Mar;29(6):1203-12
references: 18297658
references: Radiat Prot Dosimetry. 2011 Feb;143(2-4):344-8
references: 21149295
references: Biomicrofluidics. 2010 Jan 07;4(1):13202
references: 20644665
references: Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2099-104
references: 10681418
references: Lab Chip. 2011 Jan 7;11(1):33-47
references: 20944856
references: Opt Express. 2009 Mar 16;17(6):4295-305
references: 19293854
references: Radiat Res. 2001 Aug;156(2):210-4
references: 11448243
references: Radiat Environ Biophys. 2008 Nov;47(4):423-9
references: 18648839
references: J Microsc. 2000 Nov;200(Pt 2):83-104
references: 11106949