title: Performance of different in vitro techniques in the molecular diagnosis of peanut allergy.
abstract: BACKGROUND
  Peanut allergy is an increasingly serious disorder with a heterogeneous pattern of sensitization across different countries. In vitro diagnostic techniques may help in establishing these patterns.
  
  
  OBJECTIVE
  To analyze the usefulness of determining specific immunoglobulin E (sIgE) with the ImmunoCAP fluorescence enzyme immunoassay (FEIA), the ImmunoCAP ISAC CRD103 microarray (ISAC), and the basophil activation test (BAT) in the molecular diagnosis of peanut allergy.
  
  
  METHODS
  In 26 peanut-allergic patients, sIgE antibodies against allergic components were measured with FEIA, ISAC, and BAT.
  
  
  RESULTS
  The major peanut component in our population wasAra h 9.The detection of sIgE toAra h 9 using FEIA and BAT with this allergen yielded a sensitivity of 92% and 88% and a specificity of 95% and 100%, respectively. Overall diagnosis of peanut allergy by ISAC showed a sensitivity of 11% but a specificity of 95% since Ara h 9 was not present in the microarray version used. There was diagnostic agreement between the 3 techniques for the peanut allergens studied.
  
  
  CONCLUSIONS
  The determination of sIgE to Ara h 9 using FEIA and BAT offers high sensitivity and specificity in the diagnosis of peanut allergy in the Spanish population. The CRD103 version of ISAC is not of value in our region as it does not include the most common allergen, Ara h 9.
authors: Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Pamplona, Spain.
authors: G
authors: G
authors: Javaloyes
authors: M J
authors: MJ
authors: Goikoetxea
authors: I
authors: I
authors: García Núñez
authors: M L
authors: ML
authors: Sanz
authors: M
authors: M
authors: Blanca
authors: S
authors: S
authors: Scheurer
authors: S
authors: S
authors: Vieths
authors: M
authors: M
authors: Ferrer
mesh_terms: D000328:Adult; D015549:Basophil Degranulation Test / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D066298:In Vitro Techniques; D008297:Male; D021183:Peanut Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D007073:Immunoglobulin E