title: A neutral envelope-type nanoparticle containing pH-responsive and SS-cleavable lipid-like material as a carrier for plasmid DNA.
abstract: SS-cleavable proton-activated lipid-like material (ssPalm) functions as a key element in a lipid nanoparticle in which pDNA is encapsulated. The ssPalm contains dual sensing motifs that can respond to the intracellular environment; a proton-sponge unit (tertiary amines) that functions in response to an acidic environment (endosome/lysosome), and disulfide bonding that can be cleaved in a reducing environment (cytosol).
authors: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita-Ku, Sapporo, Hokkaido, 060-0812, Japan. akita@pharm.hokudai.ac.jp.
authors: Hidetaka
authors: H
authors: Akita
authors: Ryohei
authors: R
authors: Ishiba
authors: Hiroto
authors: H
authors: Hatakeyama
authors: Hiroki
authors: H
authors: Tanaka
authors: Yusuke
authors: Y
authors: Sato
authors: Kota
authors: K
authors: Tange
authors: Masaya
authors: M
authors: Arai
authors: Kazuhiro
authors: K
authors: Kubo
authors: Hideyoshi
authors: H
authors: Harashima
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D011522:Protons; D012235:Rhodamines / Q000737:chemistry; D014162:Transfection
chemical_list: D004220:Disulfides; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D011522:Protons; D012235:Rhodamines; D004247:DNA
keywords: cleavable material; gene delivery; lipid-like materials; neutralized particle; non-viral vector