title: Nanostructures of cationic amphiphilic cyclodextrin complexes with DNA.
abstract: Complexes of cationic amphiphilic cyclodextrins heptakis[2-(ω-amino-oligo(ethylene glycol))-6-deoxy-6-hexadecylthio]-β-cyclodextrin and heptakis[2-(ω-amino-oligo(ethylene glycol))-6-deoxy-6-dodecylthio]-β-cyclodextrin with DNA were examined by small-angle X-ray scattering and dynamic as well as electrophoretic light scattering. The first cyclodextrin forms bilayer vesicles in water, which, in the presence of calf thymus DNA, transform to a multilamellar complex. In this complex, the DNA lies between the two polar layers of the cyclodextrin's protonated amino groups in alternation with the lipidic bilayers. The cyclodextrin with shorter lipid chains, in contrast, forms micelles in water, and electrostatic clustering of these about DNA does not affect their intrinsic structure. These results are relevant to the potential of such cyclodextrins in therapeutic gene delivery, showing that their self-assembly modes in isolation influence their complex formation with DNA and possibly their efficiency in promoting cell transfection.
authors: CNR-IPCF Istituto per i Processi Chimico-Fisici, Viale F. Stagno d'Alcontres 37, I-98158, Messina, Italy. villari@me.cnr.it
authors: Valentina
authors: V
authors: Villari
authors: Antonino
authors: A
authors: Mazzaglia
authors: Raphael
authors: R
authors: Darcy
authors: Caitriona M
authors: CM
authors: O'Driscoll
authors: Norberto
authors: N
authors: Micali
mesh_terms: D002412:Cations; D003505:Cyclodextrins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D018014:Gene Transfer Techniques; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry; D008823:Micelles; D049329:Nanostructures / Q000737:chemistry*; D055672:Static Electricity; D014162:Transfection; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D002412:Cations; D003505:Cyclodextrins; D008051:Lipid Bilayers; D008823:Micelles; D047392:beta-Cyclodextrins; D014867:Water; D004247:DNA; C427203:calf thymus DNA; C031215:betadex