title: Patch-clamp recordings from lateral line neuromast hair cells of the living zebrafish.
abstract: Zebrafish are popular models for biological discovery. For investigators of the auditory and vestibular periphery, manipulations of hair cell and synaptic mechanisms have relied on inferences from extracellular recordings of physiological activity. We now provide data showing that hair cells and supporting cells of the lateral line can be directly patch-clamped, providing the first recordings of ionic channel activity, synaptic vesicle release, and gap junctional coupling in the neuromasts of living fish. Such capabilities will allow more detailed understanding of mechano-sensation of the zebrafish.
authors: Department of Otolaryngology, Stanford University School of Medicine, Stanford, California 94305, USA.
authors: Anthony J
authors: AJ
authors: Ricci
authors: Jun-Ping
authors: JP
authors: Bai
authors: Lei
authors: L
authors: Song
authors: Caixia
authors: C
authors: Lv
authors: David
authors: D
authors: Zenisek
authors: Joseph
authors: J
authors: Santos-Sacchi
mesh_terms: D000818:Animals; D055724:Electrophysiological Phenomena; D005260:Female; D007839:Functional Laterality / Q000502:physiology; D017629:Gap Junctions / Q000502:physiology; D006198:Hair Cells, Auditory / Q000502:physiology*; D007814:Larva; D053403:Lateral Line System / Q000294:innervation* / Q000502:physiology*; D008297:Male; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D013572:Synaptic Vesicles / Q000502:physiology; D015027:Zebrafish
chemical_list: D015221:Potassium Channels