title: Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals.
abstract: It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study.
authors: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, PR China.
authors: Ting
authors: T
authors: Wei
authors: Chao
authors: C
authors: Zhang
authors: Xin
authors: X
authors: Xu
authors: Michelle
authors: M
authors: Hanna
authors: Xiaohua
authors: X
authors: Zhang
authors: Yan
authors: Y
authors: Wang
authors: Heping
authors: H
authors: Dai
authors: Wei
authors: W
authors: Xiao
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000633:toxicity; D015374:Biosensing Techniques / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects; D004271:DNA, Fungal / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D015386:Hazardous Substances / Q000032:analysis* / Q000633:toxicity; D009153:Mutagens / Q000032:analysis* / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D000970:Antineoplastic Agents; D004271:DNA, Fungal; D015386:Hazardous Substances; D009153:Mutagens; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins