title: Methods for studies of protein interactions with different DNA methyltransferases.
abstract: There are now many methods available for studying protein interactions between DNA methltransferases (DNMTs) and their binding partners. Here we describe a step-by-step procedure to identify whether proteins of interest interact with DNMTs by co-immunoprecipitation (co-IP) assay in transiently transfected cells. Though one mammalian cell is described, investigators can use the same method with other cell lines or primary cells for in vivo protein interaction studies.
authors: Human Health and Environment Program, Desert Research Institute (DRI), Las Vegas, NV, USA.
authors: Jianchang
authors: J
authors: Yang
mesh_terms: D002458:Cell Fractionation; D002846:Chromatography, Affinity; D015254:DNA Modification Methylases / Q000302:isolation & purification* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D014162:Transfection
chemical_list: D011993:Recombinant Fusion Proteins; D015254:DNA Modification Methylases