title: Clinical factors influencing phenotype of HCMV-specific CD8+ T cells and HCMV-induced interferon-gamma production after allogeneic stem cells transplantation.
abstract: Human cytomegalovirus (HCMV) infection causes significant morbidity and mortality after hematopoietic stem cell transplantation (HSCT). In this work, we characterized the phenotype and interferon-gamma (INF-γ) production of HCMV-specific T cells using QuantiFERON-HCMV assay in 26 patients 6 months after HSCT. We analysed whether these two parameters were associated with clinical variables. Our results showed that the patients receiving stem cells from donors ≥40 years old were 12 times more likely to have HCMV-specific CD8+ T cells with "differentiated phenotype" (CD45RA+CCR7+ ≤6.7% and CD28+ ≤30%) than patients grafted from donors <40 years old (OR = 12; P = 0.014). In addition, a detectable IFN-γ production in response to HCMV peptides (cutoff 0.2 IU/mL IFN-γ; "reactive" QuantiFERON-HCMV test) was statistically associated with HCMV replication after transplantation (OR = 11; P = 0.026), recipients ≥40 versus <40 years old (OR = 11; P = 0.026), and the use of peripheral blood versus bone marrow as stem cell source (OR = 17.5; P = 0.024). In conclusion, donor age is the only factor significantly associated with the presence of the "differentiated phenotype" in HCMV-specific CD8+ T cells, whereas HCMV replication after transplantation, recipient age, and stem cell source are the factors associated with the production of IFN-γ in response to HCMV epitopes.
authors: Instituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, Spain. b62gacai@hotmail.es
authors: Inmaculada
authors: I
authors: Gayoso
authors: Sara
authors: S
authors: Cantisán
authors: Carolina
authors: C
authors: Cerrato
authors: Joaquín
authors: J
authors: Sánchez-García
authors: Carmen
authors: C
authors: Martin
authors: Rafael
authors: R
authors: Solana
authors: Antonio
authors: A
authors: Torres-Gomez
authors: Julian
authors: J
authors: Torre-Cisneros
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000276:immunology; D018106:CD28 Antigens / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D011183:Postoperative Complications / Q000276:immunology* / Q000503:physiopathology; D054400:Receptors, CCR7 / Q000378:metabolism; D033581:Stem Cell Transplantation; D014019:Tissue Donors; D014184:Transplantation, Homologous; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; C515370:CCR7 protein, human; D018106:CD28 Antigens; D018984:Epitopes, T-Lymphocyte; D054400:Receptors, CCR7; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens