title: A magnetic nanoparticles-zinc oxide/zinc hexacyanoferrate hybrid film for amperometric determination of tyrosine.
abstract: A method is described for the construction of a highly sensitive amperometric sensor for the detection of tyrosine, employing a magnetic nanoparticles-zinc oxide/zinc hexacyanoferrate (Fe3O4NP-ZnO/ZnHCF) hybrid film electrodeposited on the surface of a Pt electrode as working electrode. The sensor is based on electrocatalytic mechanism initiated by electrochemical oxidation of the reduced form of the hybrid film at +0.2 V vs. Ag/AgCl followed by completion of chemical oxidation of tyrosine. The sensor showed optimum response within 2 s at pH 2. The working/linear range of the sensor was 0.02-2.76 mM with a detection limit of 4 μM. The sensor measured tyrosine level in serum, a potential biomarker of phenylketonuria. The working electrode lost only 5 % of its initial activity, when stored at 4 °C, after its regular use over a period of 100 days.
authors: Department of Biochemistry, M. D. University, Rohtak, 124 001, Haryana, India.
authors: Jagriti
authors: J
authors: Narang
authors: Nidhi
authors: N
authors: Chauhan
authors: Shikha
authors: S
authors: Pundir
authors: C S
authors: CS
authors: Pundir
mesh_terms: D002384:Catalysis; D055664:Electrochemical Techniques / Q000379:methods*; D005295:Ferrocyanides / Q000737:chemistry*; D057230:Limit of Detection; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D014443:Tyrosine / Q000032:analysis*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D005295:Ferrocyanides; C116383:ferrocyn; D014443:Tyrosine; D015034:Zinc Oxide