title: [Protective role of calcium alginate hydrogel beads in cells during calcium phosphate cement setting reaction and its influencing factors].
abstract: OBJECTIVE
  To evaluate the protection of calcium alginate hydrogel beads for mouse preosteoblastic cell line (MC3T3-E1) during the setting reaction  of calcium phosphate cement (CPC) pastes and to analyze related influencing factors.
  
  
  METHODS
  The MC3T3-E1 cells encapsulated in alginate  hydrogel beads were cultured in vitro with β-tricalcium phosphate/calcium phosphate cement (β-TCP/CPC) pastes and discs until 2 d. The cell viability  and number of live/dead cells were tested using the CCK-8, Calcein-AM and PI, respectively. The effects of CPC paste and CPC disc, different treatment  and time to renewal medium on cell viability were compared.
  
  
  RESULTS
  There were no significant differences in optical density and number of live cells  between β-TCP/CPC pastes and discs (1 d: P=0.827; 2 d: P=0.965). There were different influences on cell viability between different treatment and time to  renewal medium.
  
  
  CONCLUSIONS
  The results indicated that the calcium alginate hydrogel beads had a good protection to MC3T3-E1 cells during the  setting reaction of CPC pastes. CPC paste might affect the cell proliferation even when solidification. After setting reaction, it may be favorable for cell  viability to renew the medium every 6 h in 1 d.
authors: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
authors: Fang-fang
authors: FF
authors: Li
authors: Peng-yan
authors: PY
authors: Qiao
authors: Li-min
authors: LM
authors: Dong
authors: Yu-hua
authors: YH
authors: Liu
authors: Tao
authors: T
authors: Xu
authors: Qiu-fei
authors: QF
authors: Xie
mesh_terms: D016475:3T3 Cells; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D020100:Hydrogels / Q000494:pharmacology; D051379:Mice; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology*; D020011:Protective Agents / Q000494:pharmacology
chemical_list: D000464:Alginates; D018786:Bone Substitutes; D002130:Calcium Phosphates; D006603:Hexuronic Acids; D020100:Hydrogels; D020011:Protective Agents; D020723:Glucuronic Acid; C020243:calcium phosphate