title: Effect of dietary copper on the growth performance, non-specific immunity and resistance to Aeromonas hydrophila of juvenile Chinese mitten crab, Eriocheir sinensis.
abstract: An 8-week feeding trial was conducted to determine the dietary copper (Cu) on growth performance and immune responses of juvenile Chinese mitten crab Eriocheir sinensis. Six semi-purified diets with six copper levels (1.88, 11.85, 20.78, 40.34, 79.56 and 381.2 mg kg(-1) diet) of CuSO4·5H2O were fed to E. sinensis (0.45 ± 0.01 g). Each diet was fed to the crab in five replicates. The crab fed diets with 20.78 and 40.34 mg Cu kg(-1) diet had significantly greater weight gain and hemolymph oxyhemocyanin content than those fed diets with 1.88 and 381.2 mg Cu kg(-1) diet. Survival rates of crab were not significantly different between all treatment groups. The activities of copper-zinc superoxide dismutase (Cu-Zn SOD), phenoloxidase (PO), and total hemocyte count (THC) significantly increased when the supplementation of dietary copper reached 20.78-40.34 mg Cu kg(-1) diets. In the bacteria challenge experiment with Aeromonas hydrophila, survival rates significantly increased and reached a plateau when the dietary copper increased from 1.88 to 40.34 mg kg(-1), whereas significantly decreased when the dietary copper increased from 40.34 to 381.2 mg kg(-1). This study indicates that the level of dietary copper is important in regulating growth and immune response in crab.
authors: School of Life Science, East China Normal University, Shanghai 200062, PR China.
authors: Shengming
authors: S
authors: Sun
authors: Jianguang
authors: J
authors: Qin
authors: Na
authors: N
authors: Yu
authors: Xianping
authors: X
authors: Ge
authors: Haibo
authors: H
authors: Jiang
authors: Liqiao
authors: L
authors: Chen
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D017756:Aquaculture; D003386:Brachyura / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology / Q000382:microbiology; D003300:Copper / Q000633:toxicity*; D006433:Hemocyanins / Q000378:metabolism; D006434:Hemocytes / Q000166:cytology; D007113:Immunity, Innate / Q000187:drug effects*; D015430:Weight Gain
chemical_list: C021724:oxy-beta-hemocyanin; D003300:Copper; D006433:Hemocyanins