title: CD36 and SR-BI are involved in cellular uptake of provitamin A carotenoids by Caco-2 and HEK cells, and some of their genetic variants are associated with plasma concentrations of these micronutrients in humans.
abstract: Scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36) have been involved in cellular uptake of some provitamin A carotenoids. However, data are incomplete (e.g., there are no data on α-carotene), and it is not known whether genetic variants in their encoding genes can affect provitamin A carotenoid status. The objectives were 1) to assess the involvement of these scavenger receptors in cellular uptake of the main provitamin A carotenoids (i.e., β-carotene, α-carotene, and β-cryptoxanthin) as well as that of preformed vitamin A (i.e., retinol) and 2) to investigate the contribution of genetic variations in genes encoding these proteins to interindividual variations in plasma concentrations of provitamin A carotenoids. The involvement of SR-BI and CD36 in carotenoids and retinol cellular uptake was investigated in Caco-2 and human embryonic kidney (HEK) cell lines. The involvement of scavenger receptor class B type I (SCARB1) and CD36 genetic variants on plasma concentrations of provitamin A carotenoids was assessed by association studies in 3 independent populations. Cell experiments suggested the involvement of both proteins in cellular uptake of provitamin A carotenoids but not in that of retinol. Association studies showed that several plasma provitamin A carotenoid concentrations were significantly different (P < 0.0083) between participants who bore different genotypes at single nucleotide polymorphisms and haplotypes in CD36 and SCARB1. In conclusion, SR-BI and CD36 are involved in cellular uptake of provitamin A carotenoids, and genetic variations in their encoding genes may modulate plasma concentrations of provitamin A carotenoids at a population level.
authors: INRA, UMR1260 Nutrition, Obesity and Risk of Thrombosis, Marseille, France. patrick.borel@univ-amu.fr
authors: Patrick
authors: P
authors: Borel
authors: Georg
authors: G
authors: Lietz
authors: Aurélie
authors: A
authors: Goncalves
authors: Fabien
authors: F
authors: Szabo de Edelenyi
authors: Sophie
authors: S
authors: Lecompte
authors: Peter
authors: P
authors: Curtis
authors: Louisa
authors: L
authors: Goumidi
authors: Muriel J
authors: MJ
authors: Caslake
authors: Elizabeth A
authors: EA
authors: Miles
authors: Christopher
authors: C
authors: Packard
authors: Philip C
authors: PC
authors: Calder
authors: John C
authors: JC
authors: Mathers
authors: Anne M
authors: AM
authors: Minihane
authors: Franck
authors: F
authors: Tourniaire
authors: Emmanuelle
authors: E
authors: Kesse-Guyot
authors: Pilar
authors: P
authors: Galan
authors: Serge
authors: S
authors: Hercberg
authors: Christina
authors: C
authors: Breidenassel
authors: Marcela
authors: M
authors: González Gross
authors: Myriam
authors: M
authors: Moussa
authors: Aline
authors: A
authors: Meirhaeghe
authors: Emmanuelle
authors: E
authors: Reboul
mesh_terms: D000293:Adolescent; D018955:CD36 Antigens / Q000235:genetics* / Q000502:physiology*; D018938:Caco-2 Cells; D002338:Carotenoids / Q000097:blood* / Q000378:metabolism*; D003430:Cross-Sectional Studies; D065366:Cryptoxanthins; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D051122:Scavenger Receptors, Class B / Q000235:genetics* / Q000502:physiology*; D012737:Sex Factors; D014801:Vitamin A / Q000378:metabolism; D024341:Xanthophylls / Q000097:blood / Q000378:metabolism; D019207:beta Carotene / Q000097:blood / Q000378:metabolism
chemical_list: D018955:CD36 Antigens; D065366:Cryptoxanthins; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; D024341:Xanthophylls; D019207:beta Carotene; D014801:Vitamin A; D002338:Carotenoids; C041635:alpha-carotene