title: Accumulation of xenotransplanted canine bone marrow cells in NOD/SCID/γc(null) mice with acute hepatitis induced by CCl4.
abstract: Bone marrow cell infusion (BMI) has recently been suggested as an effective therapy for refractory liver disease; however, the efficiency of BMI using canine bone marrow cells (cBMCs) has not been reported. We evaluated the accumulation potential of cBMCs in a mouse model of acute liver failure. Acute hepatitis was induced by carbon tetrachloride (CCl4) treatment in NOD/SCID/γc(null)(NOG) mice and wild-type (WT) C57BL mice, and the characteristics of liver dysfunction and the degree of hepatic injury and regeneration were compared between the two mouse models. Next, female CCl4-treated NOG mice were xenotransplanted with male PKH26-labeled cBMCs, and the potential of cBMCs to accumulate in injured liver tissue compartments was examined. Fluorescence microscopy was performed to histologically detect the infused cBMCs, and DNA polymerase chain reaction was performed for detection of the male Y chromosome (SRY gene) in the recipient female NOG mice. The number of PKH26-positive cBMCs transplanted in the liver tissue gradually increased in the NOG mice. The infused cBMCs were located in the necrotic area of the liver at an early stage after transplantation, and most had accumulated a week after transplantation. However, the therapeutic efficacy of the xenotransplantation remained unclear, because no significant differences were observed concerning the extent liver injury and regeneration between the cBMC-transplanted and saline control mice. These results suggest that cBMCs will specifically accumulate in injured liver tissue and that BMC transplantation may have the potential to repair liver deficiency.
authors: Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252-5201, Japan.
authors: Takashi
authors: T
authors: Kato
authors: Masaharu
authors: M
authors: Hisasue
authors: Kazuhito
authors: K
authors: Segawa
authors: Ayumi
authors: A
authors: Fujimoto
authors: Eri
authors: E
authors: Makiishi
authors: Sakurako
authors: S
authors: Neo
authors: Kyohei
authors: K
authors: Yasuno
authors: Ryosuke
authors: R
authors: Kobayashi
authors: Ryo
authors: R
authors: Tsuchiya
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D004285:Dogs; D005260:Female; D024282:Genes, sry / Q000235:genetics; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D014183:Transplantation, Heterologous / Q000379:methods*
chemical_list: D002251:Carbon Tetrachloride