title: Endothelin-1-induced down-regulation of NaV1.7 expression in adrenal chromaffin cells: attenuation of catecholamine secretion and tau dephosphorylation.
abstract: Endothelin-1 and voltage-dependent sodium channels are involved in control and suppression of neuropathological factors, which contribute to sculpting the neuronal network. We previously demonstrated that veratridine-induced NaV1.7 sodium channel activation caused intracellular calcium elevation, catecholamine secretion and tau dephosphorylation in adrenal chromaffin cells. The aim of this study was to examine whether endothelin-1 could modulate NaV1.7. Our results indicated that endothelin-1 decreased the protein level of NaV1.7 and the veratridine-induced increase in intracellular calcium. In addition, it also abolished the veratridine-induced dephosphorylation of tau and the phosphorylation of glycogen synthase kinase-3β and extracellular signal-regulated kinase. These findings suggest that the endothelin-1-induced down-regulation of NaV1.7 diminishes NaV1.7-related catecholamine secretion and dephosphorylation of tau.
authors: Department of Pharmacology, Miyazaki Medical College, University of Miyazaki, Miyazaki, Japan. tnemoto@fc.miyazaki-u.ac.jp
authors: Takayuki
authors: T
authors: Nemoto
authors: Toshihiko
authors: T
authors: Yanagita
authors: Toyoaki
authors: T
authors: Maruta
authors: Chihiro
authors: C
authors: Sugita
authors: Shinya
authors: S
authors: Satoh
authors: Tasuku
authors: T
authors: Kanai
authors: Akihiko
authors: A
authors: Wada
authors: Manabu
authors: M
authors: Murakami
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D019332:Endothelin-1 / Q000494:pharmacology*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D007150:Immunohistochemistry; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D062556:NAV1.7 Voltage-Gated Sodium Channel / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D013997:Time Factors; D014701:Veratridine / Q000494:pharmacology; D014966:Xanthenes; D016875:tau Proteins / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D002395:Catecholamines; D019332:Endothelin-1; C409648:Fluo 4; D062556:NAV1.7 Voltage-Gated Sodium Channel; D014966:Xanthenes; D016875:tau Proteins; D014701:Veratridine; D000071679:Glycogen Synthase Kinase 3 beta; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D038362:Glycogen Synthase Kinase 3; D002118:Calcium