title: Photonic crystal biosensor based on optical surface waves.
abstract: A label-free biosensor device based on registration of photonic crystal surface waves is described. Angular interrogation of the optical surface wave resonance is used to detect changes in the thickness of an adsorbed layer, while an additional simultaneous detection of the critical angle of total internal reflection provides independent data of the liquid refractive index. The abilities of the device are demonstrated by measuring of biotin molecule binding to a streptavidin monolayer, and by measuring association and dissociation kinetics of immunoglobulin G proteins. Additionally, deposition of PSS / PAH polyelectrolytes is recorded in situ resulting calculation of PSS and PAH monolayer thicknesses separately.
authors: Institute of Spectroscopy, Russian Academy of Sciences, Fizicheskaya, 5, Troitsk, Moscow Region, 142190, Russia. konopsky@gmail.com
authors: Valery N
authors: VN
authors: Konopsky
authors: Tanya
authors: T
authors: Karakouz
authors: Elena V
authors: EV
authors: Alieva
authors: Chiara
authors: C
authors: Vicario
authors: Sergey K
authors: SK
authors: Sekatskii
authors: Giovanni
authors: G
authors: Dietler
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D001710:Biotin / Q000378:metabolism; D019921:Biotinylation; D003460:Crystallization; D004573:Electrolytes / Q000737:chemistry; D055767:Immobilized Proteins / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D008024:Ligands; D051379:Mice; D055095:Optics and Photonics / Q000295:instrumentation*; D011817:Rabbits; D012815:Signal Processing, Computer-Assisted; D019809:Streptavidin / Q000378:metabolism; D013499:Surface Properties
chemical_list: D004573:Electrolytes; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D008024:Ligands; D001710:Biotin; D019809:Streptavidin