title: 2-(Benzothiazol-2-yl)-phenyl-β-d-galactopyranoside derivatives as fluorescent pigment dyeing substrates and their application for the assay of β-d-galactosidase activities.
abstract: 2-(Benzothiazol-2-yl)-phenyl-β-d-galactopyranoside derivatives were synthesized as novel artificial fluorescent pigment dyeing substrates for β-d-galactosidase. The substrates, which exhibited non-fluorescence or weak fluorescence in solution phase, were smoothly hydrolyzed by β-d-galactosidase from Aspergillus oryzae and yielded a water-insoluble strong fluorescent pigment. The difference of fluorescent intensity exhibited a linear relationship with the amount of enzyme.
authors: Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Hiroshima International University, 5-1-1 Hirokoshingai, Kure, Hiroshima 737-0112, Japan. t-ootubo@ps.hirokoku-u.ac.jp
authors: Tadamune
authors: T
authors: Otsubo
authors: Akira
authors: A
authors: Minami
authors: Haruna
authors: H
authors: Fujii
authors: Risa
authors: R
authors: Taguchi
authors: Tadanobu
authors: T
authors: Takahashi
authors: Takashi
authors: T
authors: Suzuki
authors: Fumiteru
authors: F
authors: Teraoka
authors: Kiyoshi
authors: K
authors: Ikeda
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology; D052160:Benzothiazoles / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005697:Galactosides / Q000737:chemistry*; D015394:Molecular Structure; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D001616:beta-Galactosidase / Q000032:analysis* / Q000378:metabolism
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D005697:Galactosides; D001616:beta-Galactosidase