title: Simultaneous determination of evodiamine and evodine in Beagle dog plasma using liquid chromatography tandem mass spectrometry.
abstract: A sensitive, rapid, and specific liquid chromatography/tandem mass spectrometry assay has been established and validated for the quantitation of evodiamine and evodine in Beagle dog plasma. Plasma samples of 0.2 ml were processed by liquid-liquid extraction with n-hexane/ethyl acetate (2:1, v/v). Chromatographic separations were done on a Symmetry C18 column (100 mm × 4.6 mm, ID, 5 μm) at 35°C with a linear gradient of methanol and 20 mM ammonium formate containing 0.2% formic acid. Evodiamine, evodine, and glibenclamide [internal standard (IS)] were ionized with an electrospray ionization source operated in positive ion mode. The MS/MS transitions were m/z 304.1 → 161.1 for evodiamine, m/z 471.2 → 425.1 for evodine, and m/z 494.1 → 369.1 for IS. Calibration curves were linear over the concentration range of 0.1-100 ng/ml for evodiamine and 0.5-500 ng/ml for evodine. The mean extraction recoveries were 88.10 ± 3.21% for evodiamine and 81.24 ± 4.07% for evodine. The intra- and inter-day precisions were less than 11.10% and 12.81%, and the accuracy was within ± 11.76% for both analytes. Evodiamine and evodine were stable during storage and analytical periods. The validated method has been successfully applied to a pharmacokinetic study of evodiamine and evodine in beagle dogs after oral administration.
authors: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Yuan-Yuan
authors: YY
authors: Xia
authors: Hai-Yu
authors: HY
authors: Xu
authors: You-You
authors: YY
authors: Cai
authors: Duan-Yun
authors: DY
authors: Si
authors: Chang-Xiao
authors: CX
authors: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D004285:Dogs; D005663:Furans / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D011799:Quinazolines / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005663:Furans; D006576:Heterocyclic Compounds, 4 or More Rings; D011799:Quinazolines; C473746:evodine; C049639:evodiamine