title: White adipose tissue overproduces the lipid-mobilizing factor zinc α2-glycoprotein in chronic kidney disease.
abstract: Chronic kidney disease (CKD) is frequently associated with protein-energy wasting, a recognized strong predictive factor of mortality. Zinc α2-glycoprotein (ZAG) is a new adipokine involved in body weight control through its lipid-mobilizing activity. Here we tested whether the uremic environment in CKD could alter ZAG production by white adipose tissue and contribute to CKD-associated metabolic disturbances. Compared with normal plasma, uremic plasma induced a significant increase in ZAG synthesis (124%), was associated with a significant increase in basal lipolysis (31%), and significantly blunted lipogenesis (-53%) in 3T3-L1 adipocytes in vitro. In 5/6 nephrectomized rats and mice in vivo, there was a significant decrease in white adipose tissue accretion (-44% and -43%, respectively) and a significantly higher white adipose tissue content of ZAG protein than in sham-operated, pair-fed control animals (498% and 106%, respectively). Subcutaneous white adipose tissue biopsies from patients with end-stage renal disease exhibited a higher content of ZAG (573%) than age-matched controls. Thus, the ZAG content is increased in white adipose tissue from patients or animal models with CKD. Overproduction of ZAG in CKD could be a major contributor to metabolic disturbances associated with CKD.
authors: Université de Lyon, Lyon, France.
authors: Caroline C
authors: CC
authors: Pelletier
authors: Laetitia
authors: L
authors: Koppe
authors: Marine L
authors: ML
authors: Croze
authors: Emilie
authors: E
authors: Kalbacher
authors: Roxane E
authors: RE
authors: Vella
authors: Fitsum
authors: F
authors: Guebre-Egziabher
authors: Alain
authors: A
authors: Géloën
authors: Lionel
authors: L
authors: Badet
authors: Denis
authors: D
authors: Fouque
authors: Christophe O
authors: CO
authors: Soulage
mesh_terms: D041721:3T3-L1 Cells; D054392:Adipokines; D052436:Adipose Tissue, White / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001706:Biopsy; D002352:Carrier Proteins / Q000097:blood*; D016022:Case-Control Studies; D004195:Disease Models, Animal; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D050155:Lipogenesis; D008066:Lipolysis; D008297:Male; D051379:Mice; D008875:Middle Aged; D010530:Peritoneal Dialysis; D051381:Rats; D017208:Rats, Wistar; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000628:therapy; D015854:Up-Regulation; D014511:Uremia / Q000097:blood; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D054392:Adipokines; D002352:Carrier Proteins; D006023:Glycoproteins; C488891:AZGP1 protein, human; D000090203:Zn-Alpha-2-Glycoprotein