title: [Three dimensional bioprinting technology of human dental pulp cells mixtures].
abstract: OBJECTIVE
  To explore the three dimensional(3D)bioprinting technology, using human dental pulp cells (hDPCs) mixture as bioink and to lay initial  foundations for the application of the 3D bioprinting technology in tooth regeneration.
  
  
  METHODS
  Imageware 11.0 computer software was used to aid the  design of the 3D biological printing blueprint. Sodium alginate-gelatin hydrosol was prepared and mixed with in vitro isolated hDPCs. The mixture  contained 20 g/L sodium alginate and 80 g/L gelatin with cell density of 1×10(6)/mL. The bioprinting of hDPCs mixture was carried out according to certain  parameters; the 3D constructs obtained by printing were examined; the viability of hDPCs after printing by staining the constructs with calcein-AM and  propidium iodide dye and scanning of laser scanning confocal microscope was evaluated. The in vitro constructs obtained by the bioprinting were  cultured, and the proliferation of hDPCs in the constructs detected.
  
  
  RESULTS
  By using Imageware 11.0 software, the 3D constructs with the grid structure  composed of the accumulation of staggered cylindrical microfilament layers were obtained. According to certain parameters, the hDPCs-sodium alginate-gelatin blends were printed by the 3D bioprinting technology. The self-defined shape and dimension of 3D constructs with the cell survival rate of 87%± 2% were constructed. The hDPCs could proliferate in 3D constructs after printing.
  
  
  CONCLUSIONS
  In this study, the 3D bioprinting of hDPCs mixtures was  realized, thus laying initial foundations for the application of the 3D bioprinting technology in tooth regeneration.
authors: Peking University School, Beijing, China.
authors: Shi-hua
authors: SH
authors: Xue
authors: Pei-jun
authors: PJ
authors: Lv
authors: Yong
authors: Y
authors: Wang
authors: Yu
authors: Y
authors: Zhao
authors: Ting
authors: T
authors: Zhang
mesh_terms: D000464:Alginates / Q000737:chemistry; D062028:Bioprinting / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D017076:Computer-Aided Design; D003782:Dental Pulp / Q000166:cytology*; D005780:Gelatin; D048091:Guided Tissue Regeneration; D006801:Humans; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000502:physiology
chemical_list: D000464:Alginates; D005780:Gelatin