title: Immobilization of Yarrowia lipolytica for aroma production from castor oil.
abstract: The main aim of this study was to compare different materials for Y. lipolytica immobilization that could be used in the production of γ-decalactone (a peach-like aroma) in order to prevent the toxic effect both of the substrate and the aroma upon the cells. Therefore, cells adsorption onto pieces of methyl polymethacrylate and of DupUM(®) was studied and further used in the biotransformation of castor oil into γ-decalactone. The highest aroma concentration was obtained with immobilized cells in DupUM(®), where reconsumption of the aroma by the cells was prevented, contrarily to what happens with free cells. This is a very promising result for γ-decalactone production, with potential to be used at an industrial level since the use of immobilized cells system will facilitate the conversion of a batch process into a continuous mode keeping high cell density and allowing easier recovery of metabolic products.
authors: IBB-Institute for Biotechnology and Bioengineering, Center of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors: Adelaide
authors: A
authors: Braga
authors: Isabel
authors: I
authors: Belo
mesh_terms: D001711:Biotransformation; D002368:Castor Oil / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism*; D007783:Lactones / Q000378:metabolism*; D025062:Yarrowia / Q000378:metabolism*
chemical_list: D007783:Lactones; C109757:decan-4-olide; D002368:Castor Oil