title: Alteration of α-tocopherol-associated protein (TAP) expression in human breast epithelial cells during breast cancer development.
abstract: Breast cancer is the most common malignancy among women and has an age-specific incidence profile. Over the last decade, many studies have demonstrated the anticancer activity of α-tocopherol, the main and most active form of natural vitamin E. α-Tocopherol-associated protein (TAP) was found to be one of the major α-tocopherol binding proteins in human serum and in liver, brain, and prostate tissues. In this study, we found that reduced TAP expression was significantly correlated with Her2/neu receptor expression, breast cancer stage and nodal stage in paired normal and cancerous breast tissue samples from 93 patients using real-time PCR analysis. A cell viability assay showed that α-tocopheryl succinate (α-TOS), a synthetic derivative of α-tocopherol, enhanced the cells' sensitivity to doxorubicin and resulted in a reduction in cell viability in breast cancers. Taken together, these data suggest that the use of vitamin E or its analogue as a dietary supplement may be beneficial for the treatment of cancer.
authors: Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors: Ka-Wai
authors: KW
authors: Tam
authors: Chi-Tang
authors: CT
authors: Ho
authors: Wen-Jui
authors: WJ
authors: Lee
authors: Shih-Hsin
authors: SH
authors: Tu
authors: Ching-Shui
authors: CS
authors: Huang
authors: Ching-Shyang
authors: CS
authors: Chen
authors: Chia-Hwa
authors: CH
authors: Lee
authors: Chih-Hsiung
authors: CH
authors: Wu
authors: Yuan-Soon
authors: YS
authors: Ho
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D019587:Dietary Supplements / Q000032:analysis; D018450:Disease Progression; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage; D024502:alpha-Tocopherol / Q000008:administration & dosage
chemical_list: D002352:Carrier Proteins; D008074:Lipoproteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D015534:Trans-Activators; D014810:Vitamin E; D024502:alpha-Tocopherol; C432422:SEC14L2 protein, human