title: Gene delivery into human cancer cells by cationic lipid-mediated magnetofection.
abstract: In this study, a combination of magnetic nanoparticles (MNPs) together with cationic lipid N,N-di-n-hexadecyl-N,N-dihydroxyethylammonium chloride formulated with colipid cholesterol, upon magnetofection, enhanced DNA uptake into human glioblastoma-astrocytoma, epithelial-like cell line U-87 MG, hepatocellular carcinoma Hep G2, cervical cancer HeLa and breast cancer MDA-MB-231 cells. Having confirmed this, we monitored uptake of plasmid DNA mediated by ternary magnetoplexes by fluorescence microscopy, flow cytometry and reporter gene expression assays in the presence and absence of a magnetic field. Our observations clearly indicate enhanced transfection efficiency in vitro, upon magnetofection, in the presence of serum as seen from β-Gal reporter gene expression. The observed activity in serum suggests the suitability of MNPs for in vivo applications. Further, we measured the transverse relaxation time (T2) and obtained T2-weighted MRI images of treated U-87 MG cells. T2 determined for MNP-VP-Me22 and MNP-VP-Et22 corresponds to 22.6±0.8 ms and 36.0±2.1 ms, respectively, as compared to 47±1.7 ms for control, suggesting their applicability in molecular imaging. Our results collectively highlight the potential of lipid-based approach to augment magnetic-field guided-gene delivery using MNPs and additionally towards developing intracellular molecular probes for magnetic resonance imaging.
authors: CSIR-Centre for Cellular and Molecular Biology (Council of Scientific and Industrial Research), Uppal Road, Hyderabad 500 007, Andhra Pradesh, India.
authors: Srinath
authors: S
authors: Govindarajan
authors: Kumiko
authors: K
authors: Kitaura
authors: Makoto
authors: M
authors: Takafuji
authors: Hirotaka
authors: H
authors: Ihara
authors: K S
authors: KS
authors: Varadarajan
authors: Anant B
authors: AB
authors: Patel
authors: Vijaya
authors: V
authors: Gopal
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D004983:Ethanolamines / Q000737:chemistry*; D006801:Humans; D008081:Liposomes; D060328:Magnetic Phenomena; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D004983:Ethanolamines; D008081:Liposomes; C400066:N,N-di-n-hexadecyl-N,N-dihydroxyethylammonium; D000644:Quaternary Ammonium Compounds; D004247:DNA