title: Enhanced biotransformation of 1,3-dichloro-2-propanol to epichlorohydrin via resin-based in situ product removal process.
abstract: Biotransformation of 1,3-dichloro-2-propanol (DCP) to epichlorohydrin (ECH) by the whole cells of recombinant Escherichia coli expressing halohydrin dehalogenase was limited by product inhibition. To solve this problem and improve the ECH yield, a biotransformation strategy using resin-based in situ product removal (ISPR) was investigated. Seven macroporous resins were examined to adsorb ECH: resin HZD-9 was the best. When 10 % (w/v) HZD-9 was added to batch biotransformation, 53.3 mM ECH was obtained with a molar yield of 88.3 %. The supplement of the HZD-9 increased the ECH volumetric productivity from 0.5 to 2.8 mmol/l min compared to without addition of resin. In fed-batch biotransformation, this approach increased ECH from 31 to 87 mM. These results provide a promising basis for the biosynthesis of ECH.
authors: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China. zousp@zjut.edu.cn
authors: Shu-Ping
authors: SP
authors: Zou
authors: Er-Hong
authors: EH
authors: Du
authors: Zhong-Ce
authors: ZC
authors: Hu
authors: Yu-Guo
authors: YG
authors: Zheng
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism; D004811:Epichlorohydrin / Q000302:isolation & purification* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000517:alpha-Chlorohydrin / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D011108:Polymers; D011994:Recombinant Proteins; D004811:Epichlorohydrin; C033489:1,3-dichloro-2-propanol; D000517:alpha-Chlorohydrin; D006867:Hydrolases; C430848:halohydrin dehalogenase