title: Preparation of rodent testis co-cultures.
abstract: Male reproductive development is a complex process that is sensitive to disruption by a range of toxicants. There is a great need for in vitro models that can evaluate potential male reproductive toxicants. The current unit presents a protocol for preparation of a three-dimensional in vitro model of male reproductive development that reduces the number of animals required for evaluation of toxicants. A Matrigel overlay provides a three-dimensional extracellular matrix that improves cell attachment, viability, and communication, and makes the model more reflective of in vivo environments.
authors: Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
authors: Susanna
authors: S
authors: Wegner
authors: Sungwoo
authors: S
authors: Hong
authors: Xiaozhong
authors: X
authors: Yu
authors: Elaine M
authors: EM
authors: Faustman
mesh_terms: D000826:Animal Testing Alternatives / Q000379:methods*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D007985:Leydig Cells / Q000166:cytology / Q000187:drug effects; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects; D013737:Testis / Q000166:cytology / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods*