title: A novel manganese starvation-inducible expression system for Lactobacillus plantarum.
abstract: A novel expression system for Lactobacillus plantarum was developed. This system is based on the manganese starvation-inducible promoter from specific manganese transporter of L. plantarum NC8, which was cloned for the first time. The expression of a β-glucosidase from Pyrococcus furiosus (CelB) was achieved by cultivating L. plantarum NC8 at low manganese concentrations with MRS medium and the pmntH2-CelB expression vector. A CelB activity of 8.52 μkatoNPGal L(-1) was produced in a bioreactor (4 L). The advantages of the novel expression system are that no addition of an external inducing agent was required, and additionally, no further introduction of regulatory genes was necessary. The new promoter meets the general demands of a food-grade expression system.
authors: Institute of Food Science and Biotechnology, Department of Biotechnology, University of Hohenheim, Stuttgart, Germany.
authors: Nico
authors: N
authors: Böhmer
authors: Saskia
authors: S
authors: König
authors: Lutz
authors: L
authors: Fischer
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005516:Food Microbiology / Q000379:methods; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D060847:Metabolic Engineering; D008967:Molecular Biology / Q000379:methods*; D011401:Promoter Regions, Genetic; D020027:Pyrococcus furiosus / Q000201:enzymology / Q000235:genetics
chemical_list: D003470:Culture Media; D008345:Manganese; D005966:Glucuronidase