title: The enzyme thermistor--a realistic biosensor concept. A critical review.
abstract: This review describes principles and features of thermal biosensors and microbiosensors in flow injection analysis. Examples are given that illustrate the great versatility and excellent operational stability offered by thermal biosensors. The examples are mostly from work with the original type of enzyme thermistor operating with an enzyme column, but there will also be work described involving miniaturised devices including thermal lab-on-chip constructions and other types of sensing materials, such as MIPs (molecularly imprinted polymers) for both affinity and catalytic reactions. Several recently presented thermal biosensor concepts are reviewed including a thermal-electrochemical hybrid sensor for lactose based on immobilised cellobiose dehydrogenase. Another recent method is the determination of fructose using a fructose-6-phosphate kinase column. Operation with complex sample matrices such as blood, plasma and milk and how to avoid non-specific temperature effects are considered.
authors: Department of Biochemistry and Structural Biology, Lund University, PO Box 124, 22100 Lund, Sweden. maria.yakovleva@gmail.com
authors: Maria
authors: M
authors: Yakovleva
authors: Sunil
authors: S
authors: Bhand
authors: Bengt
authors: B
authors: Danielsson
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004798:Enzymes / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005632:Fructose / Q000032:analysis; D005636:Fructosephosphates / Q000378:metabolism; D007785:Lactose / Q000032:analysis; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry
chemical_list: D004798:Enzymes; D004800:Enzymes, Immobilized; D005636:Fructosephosphates; D011108:Polymers; D005632:Fructose; C027618:fructose-6-phosphate; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D007785:Lactose