title: Preparation and characterization of an extracellular polysaccharide produced by the deep-sea fungus Penicillium griseofulvum.
abstract: The deep-sea fungus Penicillium griseofulvum produces an extracellular polysaccharide, Ps1-1, when grown in potato dextrose-agar medium. Ps1-1 was isolated from the fermented broth using ethanol precipitation, anion-exchange and size-exclusion chromatography. Ps1-1 is a galactomannan with a molecular weight of about 20 kDa, and a molar ratio of mannose and glucose of 1.1:1.0. On the basis of one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) and mass spectroscopic analyses, Ps1-1 is composed of a long chain of galactofuranan and a mannose core. The galactofuranan chain consists of (1→5)-linked β-galactofuranose, with additional branches at C-6 consisting of (1→)-linked β-galactofuranose residues and phosphate esters. The mannan core is composed of (1→6)-linked α-mannopyranose substituted at C-2 by (1→)-linked α-mannopyranose residues, disaccharide and trisaccharide units of (1→2)-linked α-mannopyranose. The investigation demonstrated that Ps1-1 was a galactofuranose-containing galactomannan differing from previously described extracellular polysaccharides.
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors: Yin
authors: Y
authors: Chen
authors: Wenjun
authors: W
authors: Mao
authors: Baofeng
authors: B
authors: Wang
authors: Lina
authors: L
authors: Zhou
authors: Qianqun
authors: Q
authors: Gu
authors: Yanli
authors: Y
authors: Chen
authors: Chunqi
authors: C
authors: Zhao
authors: Na
authors: N
authors: Li
authors: Chunyan
authors: C
authors: Wang
authors: Jimiao
authors: J
authors: Shan
authors: Mengxia
authors: M
authors: Yan
authors: Cong
authors: C
authors: Lin
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D062610:Fungal Polysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D005690:Galactose / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000096:biosynthesis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D010407:Penicillium / Q000378:metabolism*
chemical_list: D062610:Fungal Polysaccharides; D008351:Mannans; C012990:galactomannan; D005690:Galactose