title: Novel nanostructured biodegradable polymer matrices fabricated by phase separation techniques for tissue regeneration.
abstract: Biomimetic nanostructures have a wide range of applications. In particular, biodegradable polymer nanostructures that mimic the subtleties of extracellular matrix may provide favorable cell interactions. In this study, a co-solvent system was developed to configure a thermodynamically metastable biodegradable polymer solution, from which novel nanostructured matrices subsequently formed via wet phase separation (quaternary) or a combination with thermally induced phase separation. Three-dimensional (3D) nanostructured porous matrices were further fabricated by combination with particle-leaching (100-300μm glucose). The new co-solvent system may generate matrices with reproducible nanostructures from a variety of biodegradable polymers such as poly(d,l-lactide) (PLA), poly(ε-caprolactone) (PCL) and PCL-based polyurethane. In vitro cell culture experiments were performed with mouse pre-osteoblasts (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hBM-MSC) to evaluate the osteoinductive potential of PLA nanostructures. The results showed that nanofibrous (<100nm) membranes promoted the bone-related marker gene expression and matrix mineralization of MC3T3-E1 at 14days. Nanofibrous 3D matrices seeded with hBM-MSC without osteogenic induction supplements demonstrated a 2.5-fold increase in bone matrix deposition vs. the conventional microporous matrices after 14 and 21days. Antimicrobial nanofibers were further obtained by plasma-assisted coating of chitosan on PLA nanofibers. This study reveals a platform for fabricating novel biodegradable nanofibrous architecture, with potential in tissue regeneration.
authors: No. 1, Sec. 4, Roosevelt Road, Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan. shhsu@ntu.edu.tw
authors: S-H
authors: SH
authors: Hsu
authors: S
authors: S
authors: Huang
authors: Y-C
authors: YC
authors: Wang
authors: Y-C
authors: YC
authors: Kuo
mesh_terms: D016475:3T3 Cells; D020341:Absorbable Implants; D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry*; D054457:Tissue Scaffolds
chemical_list: D011108:Polymers