title: IL-10 reduces levels of apoptosis in Toxoplasma gondii-infected trophoblasts.
abstract: BACKGROUND
  To analyze the effects of IL-10 on the HLA-G expression and the apoptosis of trophoblasts infected with Toxoplasma gondii.
  
  
  METHODS
  T. gondii-infected or uninfected human trophoblasts and immortalized human placental BeWo cells were cultured with or without human IL-10. Uninfected and infected cells without IL-10 cells served as controls. HLA-G expression was measured by real-time PCR and flow cytometry, respectively. Cells apoptosis were analyzed by flow cytometry. Apoptosis associated moleculars were measured by real-time PCR and Western bolt.
  
  
  RESULTS
  HLA-G expression was increased in the infected trophoblasts and BeWo cells compared to uninfected cells. Treatment of infected cells with IL-10 decreased HLA-G expression compared to infected cells while no change in treatment of uninfected cells compared with uninfected cells. Levels of apoptosis and apoptosis associated caspase-3 and caspase-8 decreased and c-FLIP levels increased in treated infected cells with IL-10 compared to infected cells and no difference in IL-10 treated uninfected cells compared to uninfected cells.
  
  
  CONCLUSIONS
  IL-10 regulates HLA-G expression in T. gondii-infected trophoblasts. IL-10 treatment of infected trophoblasts reduced levels of apoptosis. This may contribute to the improvement in pregnancy outcomes when women infected with T. gondii treated with IL-10.
authors: Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, People's Republic of China.
authors: Mingdong
authors: M
authors: Zhao
authors: Ruijin
authors: R
authors: Zhang
authors: Xiaoyan
authors: X
authors: Xu
authors: Yang
authors: Y
authors: Liu
authors: Haixia
authors: H
authors: Zhang
authors: Xiaoyu
authors: X
authors: Zhai
authors: Xuemei
authors: X
authors: Hu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D059951:HLA-G Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology* / Q000494:pharmacology; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014122:Toxoplasma / Q000276:immunology* / Q000502:physiology; D014327:Trophoblasts / Q000187:drug effects / Q000276:immunology* / Q000469:parasitology
chemical_list: D051017:Apoptosis Regulatory Proteins; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; D059951:HLA-G Antigens; D016753:Interleukin-10; D053148:Caspase 3; D053181:Caspase 8