title: Rational protein sequence diversification by multi-codon scanning mutagenesis.
abstract: A new method for protein sequence diversification is based on generating random codon mutations to an encoding DNA. This allows for the scanning of user-defined amino acid changes to any protein of interest, and is an alternative to traditional directed evolution strategies. This chapter describes the procedures required to apply this technology to any protein of interest. The resulting libraries can then be screened for new or improved protein function.
authors: Department of Chemistry, Virginia Commonwealth University, Richmond, VA, USA.
authors: Jia
authors: J
authors: Liu
authors: T Ashton
authors: TA
authors: Cropp
mesh_terms: D000595:Amino Acid Sequence; D003062:Codon; D004247:DNA / Q000235:genetics; D019020:Directed Molecular Evolution; D011506:Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D003062:Codon; D011506:Proteins; D004247:DNA