title: Apple polyphenols suppress antigen presentation of ovalbumin by THP-1-derived dendritic cells.
abstract: Apple polyphenol extract (AP) and procyanidin contained in AP were investigated for their immunomodulatory effects using THP-1-derived human dendritic cells (TDDCs). The expression levels of HLA-DR (MHC class II) and CD86 (costimulatory molecule) were measured as an indicator of antigen presentation in TDDCs. A significant decrease in HLA-DR expression was observed in the AP and fractionated procyanidin-treated cells in the presence of ovalbumin (OVA), but no effect on CD86 expression was observed. The uptake of OVA was not inhibited by AP treatment, and the gene expression of membrane-associated RING-CH ubiquitin E3 ligase, MARCH1, was up-regulated by AP treatment. It can therefore be presumed that AP suppresses HLA-DR expression via the ubiquitin-proteasome pathway. Furthermore, the up-regulation of IL-12 and TNF-α was found in the procyanidin trimers-treated cells in the presence of OVA. These results suggest that apple polyphenols would be an effective factor for the development of immunomodulatory agents with suppressive effects of antigen presentation.
authors: Department of Bioscience and Biotechnology, Shinshu University, 8304 Minamiminowamura, Ina, Nagano 399-4598, Japan.
authors: Shigeru
authors: S
authors: Katayama
authors: Takuya
authors: T
authors: Kukita
authors: Eri
authors: E
authors: Ishikawa
authors: Syohei
authors: S
authors: Nakashima
authors: Susumu
authors: S
authors: Masuda
authors: Tomomasa
authors: T
authors: Kanda
authors: Hiroshi
authors: H
authors: Akiyama
authors: Reiko
authors: R
authors: Teshima
authors: Soichiro
authors: S
authors: Nakamura
mesh_terms: D017951:Antigen Presentation / Q000187:drug effects*; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D015536:Down-Regulation; D006801:Humans; D018664:Interleukin-12 / Q000276:immunology; D027845:Malus / Q000737:chemistry*; D010047:Ovalbumin / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D010047:Ovalbumin