title: Amphisomal route of MHC class I cross-presentation in bacteria-infected dendritic cells.
abstract: Dendritic cells (DCs) are among the first professional APCs encountered by the obligate intracellular bacterium Chlamydia during infection. Using an established mouse bone marrow-derived DC line, we show that DCs control chlamydial infection in multiple small inclusions characterized by restricted bacterial growth, impaired cytosolic export of the virulence factor chlamydial protease-like activity factor, and interaction with guanylate-binding protein 1, a host cell factor involved in the initiation of autophagy. During maturation of infected DCs, chlamydial inclusions disintegrate, likely because they lack chlamydial protease-like activity factor-mediated protection. Released cytosolic Chlamydia are taken up by autophagosomes and colocalize with cathepsin-positive amphisomal vacuoles, to which peptide transporter TAP and upregulated MHC class I (MHC I) are recruited. Chlamydial Ags are subsequently generated through routes involving preprocessing in amphisomes via cathepsins and entry into the cytosol for further processing by the proteasome. Finally, bacterial peptides are reimported into the endosomal pathway for loading onto recycling MHC I. Thus, we unravel a novel pathway of MHC I-mediated cross-presentation that is initiated with a host cellular attack physically disrupting the parasitophorous vacuole, involves autophagy to collect cytosolic organisms into autophagosomes, and concludes with complex multistep antigenic processing in separate cellular compartments.
authors: Institute of Immunology, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institute, 17493 Greifswald-Isle of Riems, Germany.
authors: Dorothee
authors: D
authors: Fiegl
authors: Danny
authors: D
authors: Kägebein
authors: Elisabeth M
authors: EM
authors: Liebler-Tenorio
authors: Tanja
authors: T
authors: Weisser
authors: Mareen
authors: M
authors: Sens
authors: Melanie
authors: M
authors: Gutjahr
authors: Michael R
authors: MR
authors: Knittler
mesh_terms: D000818:Animals; D001343:Autophagy / Q000276:immunology; D001996:Bronchopneumonia / Q000276:immunology / Q000382:microbiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D002461:Cell Line, Transformed; D002691:Chlamydophila psittaci / Q000276:immunology* / Q000378:metabolism; D002522:Chlorocebus aethiops; D045142:Cross-Priming / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009956:Psittacosis / Q000276:immunology / Q000473:pathology
chemical_list: D015395:Histocompatibility Antigens Class I