title: [Application of HPLC-UV method for aripiprazole determination in serum].
abstract: Aripiprazole is a new drug applied in schizophrenia treatment. There are not strict indications for aripiprazole therapeutic drug monitoring. Despite, serum aripiprazole measuring would help control the drug doses effectiveness. The drug monitoring can eliminate overdosing, adverse effects and let control proper drug ingestion. The aim of the paper was to develop a simple method for aripiprazole determination in serum for therapeutic drug monitoring. High performance liquid chromatography with spectrophotometric detection (HPLC-UV) was used. Resolution was performed on LC-8 column; moving phase was solution 0,025M trimethylammonium buffer: acetonitrile (62:38). Isocratic flow was 1,2 ml/min; internal standard (IS) was promazine; monitored wavelength was lambda=214 nm. The validation parameters were: limits of linearity (LOL) 100-800 ng/ml, limit of detection (LOD) 10 ng/ml, limit of quantity (LOQ) 100 ng/ml. Coefficient of variation (CV) describing accuracy and precision didn't cross 10%. The method was useful for therapeutic drug monitoring in serum of patients treated with aripiprazole.
authors: Zakład Chemii Analitycznej, Wydział Chemii, Uniwersytet Jagielloński, Kraków.
authors: Anna
authors: A
authors: Synowiec
authors: Ewa
authors: E
authors: Gomółka
authors: Tomasz
authors: T
authors: Zyss
authors: Andrzej
authors: A
authors: Zieba
authors: Ewa
authors: E
authors: Florek
authors: Wojciech
authors: W
authors: Piekoszewski
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood* / Q000627:therapeutic use; D000068180:Aripiprazole; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D010879:Piperazines / Q000097:blood* / Q000627:therapeutic use; D015363:Quinolones / Q000097:blood* / Q000627:therapeutic use; D012015:Reference Standards; D044967:Serum / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D014150:Antipsychotic Agents; D010879:Piperazines; D015363:Quinolones; D000068180:Aripiprazole