title: Regulation of ribosome biogenesis by nucleostemin 3 promotes local and systemic growth in Drosophila.
abstract: Nucleostemin 3 (NS3) is an evolutionarily conserved protein with profound roles in cell growth and viability. Here we analyze cell-autonomous and non-cell-autonomous growth control roles of NS3 in Drosophila and demonstrate its GTPase activity using genetic and biochemical assays. Two null alleles of ns3, and RNAi, demonstrate the necessity of NS3 for cell autonomous growth. A hypomorphic allele highlights the hypersensitivity of neurons to lowered NS3 function. We propose that NS3 is the functional ortholog of yeast and human Lsg1, which promotes release of the nuclear export adapter from the large ribosomal subunit. Release of the adapter and its recycling to the nucleus are essential for sustained production of ribosomes. The ribosome biogenesis role of NS3 is essential for proper rates of translation in all tissues and is necessary for functions of growth-promoting neurons.
authors: Department of Developmental Biology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA.
authors: Tom A
authors: TA
authors: Hartl
authors: Julie
authors: J
authors: Ni
authors: Jian
authors: J
authors: Cao
authors: Kaye L
authors: KL
authors: Suyama
authors: Stephanie
authors: S
authors: Patchett
authors: Cyril
authors: C
authors: Bussiere
authors: Dan Yi
authors: DY
authors: Gui
authors: Sheng
authors: S
authors: Tang
authors: Daniel D
authors: DD
authors: Kaplan
authors: Matthew
authors: M
authors: Fish
authors: Arlen W
authors: AW
authors: Johnson
authors: Matthew P
authors: MP
authors: Scott
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D004298:Dopamine / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D056426:Genetic Loci / Q000235:genetics; D006801:Humans; D007814:Larva / Q000166:cytology / Q000254:growth & development; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D029721:Drosophila Proteins; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C475026:LSG1 protein, S cerevisiae; C584153:nucleostemin 3, Drosophila; D004298:Dopamine