title: An alternate method for DNA and RNA extraction from clotted blood.
abstract: We developed an alternative method to extract DNA and  RNA from clotted blood for genomic and molecular investigations. A combination  of the TRIzol method and the QIAamp spin column were used to extract RNA from  frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood  Mini Kit was used for DNA extraction. Extracted DNA and RNA were adequate for  gene expression analysis and copy number variation (CNV) genotyping,  respectively. The purity of the extracted RNA and DNA was in the range of  1.8-2.0, determined by absorbance ratios of A(260):A(280).  Good DNA and RNA integrity were confirmed using gel electrophoresis and  automated electrophoresis. The extracted DNA was suitable for qPCR and  microarrays for CNV genotyping, while the extracted RNA was adequate for gene  analysis using RT-qPCR.
authors: Genomics Laboratory, Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia.
authors: Z
authors: Z
authors: Zakaria
authors: S H
authors: SH
authors: Umi
authors: S S
authors: SS
authors: Mokhtar
authors: U
authors: U
authors: Mokhtar
authors: M Z
authors: MZ
authors: Zaiharina
authors: A T A
authors: AT
authors: Aziz
authors: B P
authors: BP
authors: Hoh
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D004247:DNA / Q000097:blood* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D056915:DNA Copy Number Variations; D004586:Electrophoresis / Q000379:methods; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000097:blood* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012313:RNA; D004247:DNA