title: Salicylic acid suppresses jasmonic acid signaling downstream of SCFCOI1-JAZ by targeting GCC promoter motifs via transcription factor ORA59.
abstract: Antagonism between the defense hormones salicylic acid (SA) and jasmonic acid (JA) plays a central role in the modulation of the plant immune signaling network, but the molecular mechanisms underlying this phenomenon are largely unknown. Here, we demonstrate that suppression of the JA pathway by SA functions downstream of the E3 ubiquitin-ligase Skip-Cullin-F-box complex SCF(COI1), which targets JASMONATE ZIM-domain transcriptional repressor proteins (JAZs) for proteasome-mediated degradation. In addition, neither the stability nor the JA-induced degradation of JAZs was affected by SA. In silico promoter analysis of the SA/JA crosstalk transcriptome revealed that the 1-kb promoter regions of JA-responsive genes that are suppressed by SA are significantly enriched in the JA-responsive GCC-box motifs. Using GCC:GUS lines carrying four copies of the GCC-box fused to the β-glucuronidase reporter gene, we showed that the GCC-box motif is sufficient for SA-mediated suppression of JA-responsive gene expression. Using plants overexpressing the GCC-box binding APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors ERF1 or ORA59, we found that SA strongly reduces the accumulation of ORA59 but not that of ERF1. Collectively, these data indicate that the SA pathway inhibits JA signaling downstream of the SCF(COI1)-JAZ complex by targeting GCC-box motifs in JA-responsive promoters via a negative effect on the transcriptional activator ORA59.
authors: Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, 3508 TB Utrecht, The Netherlands.
authors: Dieuwertje
authors: D
authors: Van der Does
authors: Antonio
authors: A
authors: Leon-Reyes
authors: Annemart
authors: A
authors: Koornneef
authors: Marcel C
authors: MC
authors: Van Verk
authors: Nicole
authors: N
authors: Rodenburg
authors: Laurens
authors: L
authors: Pauwels
authors: Alain
authors: A
authors: Goossens
authors: Ana P
authors: AP
authors: Körbes
authors: Johan
authors: J
authors: Memelink
authors: Tita
authors: T
authors: Ritsema
authors: Saskia C M
authors: SC
authors: Van Wees
authors: Corné M J
authors: CM
authors: Pieterse
mesh_terms: D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D059372:Nucleotide Motifs; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C499534:APETALA2 protein, Arabidopsis; D000085:Acetates; D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D018398:Homeodomain Proteins; C525049:JAS1 protein, Arabidopsis; D009687:Nuclear Proteins; C531343:ORA59 protein, Arabidopsis; D054883:Oxylipins; D010454:Peptide Termination Factors; D014157:Transcription Factors; C494978:eukaryotic release factor 1, Arabidopsis; C011006:jasmonic acid; C072239:methyl jasmonate; D020156:Salicylic Acid