title: HPLC-MTT assay: anticancer activity of aqueous garlic extract is from allicin.
abstract: A strategy using reversed-phase high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), mass spectrometry (MS), nuclear magnetic resonance (NMR), chemical synthesis, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay to identify allicin as the active anticancer compound in aqueous garlic extract (AGE) is described. Changing the pH of AGE from 7.0 to 5.0 eliminated interfering molecules and enabled a clean HPLC separation of the constituents in AGE. MTT assay of the HPLC fractions identified an active fraction. Further analysis by TLC, MS, and NMR verified the active HPLC fraction as allicin. Chemically synthesized allicin was used to provide further confirmation. The results clearly identify the active compound in AGE as allicin.
authors: Illinois Mathematics and Science Academy, Aurora, IL 60506, USA.
authors: Jenny
authors: J
authors: Lee
authors: Shalini
authors: S
authors: Gupta
authors: Jin-Sheng
authors: JS
authors: Huang
authors: Lasanthi P
authors: LP
authors: Jayathilaka
authors: Bao-Shiang
authors: BS
authors: Lee
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology; D004396:Coloring Agents; D004220:Disulfides; D005737:Garlic / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D013441:Sulfinic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D004220:Disulfides; D010936:Plant Extracts; D013441:Sulfinic Acids; D013778:Tetrazolium Salts; D013844:Thiazoles; C006452:allicin; C022616:thiazolyl blue