title: Isolation and retrieval of circulating tumor cells using centrifugal forces.
abstract: Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5-88 CTCs per mL). The presence of CD133⁺ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.
authors: Department of Electrical Engineering & Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors: Han Wei
authors: HW
authors: Hou
authors: Majid Ebrahimi
authors: ME
authors: Warkiani
authors: Bee Luan
authors: BL
authors: Khoo
authors: Zi Rui
authors: ZR
authors: Li
authors: Ross A
authors: RA
authors: Soo
authors: Daniel Shao-Weng
authors: DS
authors: Tan
authors: Wan-Teck
authors: WT
authors: Lim
authors: Jongyoon
authors: J
authors: Han
authors: Ali Asgar S
authors: AA
authors: Bhagat
authors: Chwee Teck
authors: CT
authors: Lim
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D007633:Keratins; D017493:Leukocyte Common Antigens