title: Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA.
abstract: To search for potential non-viral nucleic acids carriers, a series of novel cationic polymers, multi-armed poly(aspartate-graft-oligoethylenimine) (MP-g-OEI) copolymers were designed and synthesized by grafting different types of oligoethylenimine (OEI) to a multi-armed poly(l-aspartic acid) backbone. The as-synthesized MP-g-OEI copolymers were characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance and gel permeation chromatography. These MP-g-OEI copolymers (MP423, MP600 and MP1800) exhibited good capacity in condensing nucleic acids (pDNA or siRNA) into nanosized particles (90-150nm) with positive surface charges. Gene transfection activity of the MP-g-OEI copolymers (especially MP1800) showed improved performance compared with PEI25k in both HeLa and CHO cell lines. The silencing efficiency of MP600/siRNA and MP1800/siRNA complexes showed a superior knockdown effect in CT26 and Huh-7 cell lines. Moreover, the MP-g-OEI copolymers exhibited much lower cytotoxicity than PEI25k. Flow cytometric analysis showed that MP-g-OEI copolymers could efficiently mediate the entry of nucleic acids into cells. These results suggest that MP-g-OEI copolymers may be potential non-viral gene carriers for the delivery of nucleic acids in future gene therapy.
authors: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: X
authors: X
authors: Dong
authors: L
authors: L
authors: Lin
authors: J
authors: J
authors: Chen
authors: H
authors: H
authors: Tian
authors: C
authors: C
authors: Xiao
authors: Z
authors: Z
authors: Guo
authors: Y
authors: Y
authors: Li
authors: Y
authors: Y
authors: Wei
authors: X
authors: X
authors: Chen
mesh_terms: D001388:Aziridines / Q000737:chemistry*; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D009374:Neoplasms, Experimental / Q000737:chemistry / Q000235:genetics*; D010455:Peptides / Q000737:chemistry*; D010957:Plasmids / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D001388:Aziridines; D004337:Drug Carriers; D010455:Peptides; D034741:RNA, Small Interfering; C017645:polyaspartate; C033132:aziridine