title: Synthesis and characterization of bactericidal silver nanoparticles using cultural filtrate of simulated microgravity grown Klebsiella pneumoniae.
abstract: Silver nanoparticles were synthesized by biological method using cultural filtrate of Klebsiella pneumoniae cultured under simulated microgravity and silver nitrate solution as precursor. The nanoparticles exhibited typical plasmon absorption maximum of silver nanoparticles between 405 and 407 nm. Spherical silver nanoparticles were found to have size between 15 and 37 nm by TEM analysis. XRD pattern corresponding to planes (111), (200), (220) (311) revealed the crystalline nature of the biosynthesized silver nanoparticles. FTIR spectrum proposed stabilization of silver nanoparticles by the protein molecules present in the cultural filtrate. The silver nanoparticles exhibited high bactericidal activity against Salmonella enterica, Escherichia coli and moderate bactericidal activity against Streptococcus pyogenes.
authors: Department of Forest Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju, South Korea.
authors: Duraisamy
authors: D
authors: Kalpana
authors: Yang Soo
authors: YS
authors: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D003124:Colorimetry; D017077:Culture Media, Conditioned; D004926:Escherichia coli / Q000187:drug effects; D005374:Filtration; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D053758:Nanoparticles; D050901:Nitrate Reductase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010316:Particle Size; D019779:Salmonella enterica / Q000187:drug effects; D012834:Silver / Q000494:pharmacology*; D012835:Silver Nitrate / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013297:Streptococcus pyogenes / Q000187:drug effects; D013499:Surface Properties; D018474:Weightlessness Simulation
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D012834:Silver; D012835:Silver Nitrate; D050901:Nitrate Reductase