title: Effects of a new microbial α-amylase inhibitor protein on Helicoverpa armigera larvae.
abstract: A new microbial α-amylase inhibitor gene was cloned and characterized. The encoded, recombinant, α-amylase inhibitor protein was induced and expressed by isopropyl β-d-1-thiogalactopyranoside (IPTG) in Escherichia coli M15 cells. The effects of the α-amylase inhibitor protein on Helicoverpa armigera larvae were studied. Compared to the control, the weight of H. armigera larvae fed the diet with recombinant α-amylase inhibitor protein added at a concentration of 20 μg/g was reduced by 49.8%. The total soluble protein of H. armigera larvae fed the diet with the α-amylase inhibitor protein added was also reduced by 36.8% compared to the control. The recombinant α-amylase inhibitor protein showed inhibition activity against α-amylase of H. armigera. These results suggested that this α-amylase inhibitor protein may be a promising bioinsecticide candidate for controlling H. armigera.
authors: The Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , No. 12 Zhongguancun South Street, 100081 Beijing, People's Republic of China.
authors: Fanrong
authors: F
authors: Zeng
authors: Xiaojing
authors: X
authors: Wang
authors: Jinjie
authors: J
authors: Cui
authors: Yan
authors: Y
authors: Ma
authors: Qiannan
authors: Q
authors: Li
mesh_terms: D000383:Agriculture / Q000379:methods; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D001483:Base Sequence; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004926:Escherichia coli; D015870:Gene Expression; D007306:Insecticides; D007814:Larva / Q000201:enzymology*; D009036:Moths / Q000201:enzymology*; D010572:Pest Control, Biological / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D013302:Streptomyces / Q000235:genetics; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D007306:Insecticides; D011994:Recombinant Proteins; D000516:alpha-Amylases