title: Reyranella soli sp. nov., isolated from forest soil, and emended description of the genus Reyranella Pagnier et al. 2011.
abstract: A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated KIS14-15(T), was isolated from forest soil of Baengnyeong Island in the Yellow Sea in Korea and its taxonomic position was investigated by using a polyphasic study. Strain KIS14-15(T) grew optimally at 30 °C, at pH 6.0-7.0 and without NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain KIS14-15(T) formed a cluster with the strains of Reyranella massiliensis with a bootstrap resampling value of 100%. Strain KIS14-15(T) exhibited 16S rRNA gene sequence similarity values of 98.0% to R. massiliensis 521(T) and of less than 89% to the type strains of other taxa. The mean level of DNA-DNA relatedness between strain KIS14-15(T) and R. massiliensis KACC 16548(T) was 21% (reciprocal, 24%). The predominant ubiquinone found in strain KIS14-15(T) and R. massiliensis KACC 16548(T) was ubiquinone-10 (Q-10). The predominant fatty acids of strain KIS14-15(T) and R. massiliensis KACC 16548(T) were C(18:1)ω7c, C(18:1) 2-OH and 11-methyl C(18:1)ω7c. Total polar lipids of strain KIS14-15(T) were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain KIS14-15(T) is distinguishable from R. massiliensis. On the basis of the data presented, strain KIS14-15(T) is considered to represent a novel species of the genus Reyranella, for which the name Reyranella soli sp. nov. is proposed. The type strain is KIS14-15(T) ( = KACC 13034(T) =NBRC 108950(T)).
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors: Soo-Jin
authors: SJ
authors: Kim
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors: Jae-Hyung
authors: JH
authors: Ahn
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors: Tae-Hyung
authors: TH
authors: Lee
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors: Hang-Yeon
authors: HY
authors: Weon
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors: Seung-Beom
authors: SB
authors: Hong
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors: Soon-Ja
authors: SJ
authors: Seok
authors: Institute of Microbial Ecology and Resources, Mokwon University, Daejeon 302-729, Republic of Korea.
authors: Kyung-Sook
authors: KS
authors: Whang
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors: Soon-Wo
authors: SW
authors: Kwon
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2