title: Placental expression of microRNA-17 and -19b is down-regulated in early pregnancy loss.
abstract: OBJECTIVE
  First, to determine if microRNA-17 and -19b are expressed in villous samples at early stages of pregnancy. Second, to determine whether placental expressions of these microRNAs along with their main targets (PTEN, CREB-1, TGFβ-1 and TGFβ-RII) are altered in early pregnancy loss.
  
  
  METHODS
  Expression levels of microRNAs and mRNA targets in villous samples from early pregnancy loss (n=11) and matched normal cases (n=20) by gestational age were determined by RT-PCR.
  
  
  RESULTS
  Both microRNA-17 and -19b were expressed in all cases of normal pregnancy. They were significantly down-regulated (relative ratios: 0.35 and 0.34 respectively) in early pregnancy loss. Their main target, PTEN mRNA, was significantly up-regulated in early pregnancy loss (relative ratio: 2.6, 95%CI: 0.2-29.8). TGF-β1, CREB-1 and TGFβ-RII were not significantly different between the two groups.
  
  
  CONCLUSIONS
  microRNA-17 and -19b are expressed in early stages of pregnancy. They are down-regulated in villous samples from early pregnancy loss. We suggest that these main members of the microRNA-17-92 cluster might be involved in placental invasion and its dysregulation might also be related to other conditions characterized by defective placentation.
authors: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan. walterichard@hotmail.com
authors: Walter
authors: W
authors: Ventura
authors: Keiko
authors: K
authors: Koide
authors: Kyoko
authors: K
authors: Hori
authors: Junko
authors: J
authors: Yotsumoto
authors: Akihiko
authors: A
authors: Sekizawa
authors: Hiroshi
authors: H
authors: Saito
authors: Takashi
authors: T
authors: Okai
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D051059:PTEN Phosphohydrolase / Q000235:genetics; D010920:Placenta / Q000378:metabolism; D010929:Placentation / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C524941:MIRN17 microRNA, human; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human