title: Oxidative decarboxylation of mandelic acid derivative by recombinant Escherichia coli: a novel method of ethyl vanillin synthesis.
abstract: The benzoylformate decarboxylase gene (mdlC) from Pseudomonas putida was expressed in Escherichia coli BL21(DE3). The recombinant strain together with E. coli/pET30a-mdlB converted (S)-3-ethoxy-4-hydroxymandelic acid (S-EMA) into ethyl vanillin without ethyl vanillin degradation. 4 g ethyl vanillin/l was obtained from 10 g EMA/l within 12 h at 30 °C. This is the first report on the biotransformation of (S)-EMA to ethyl vanillin.
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
authors: Xiao-Xia
authors: XX
authors: Pan
authors: Jing-Jing
authors: JJ
authors: Li
authors: Mei-Gui
authors: MG
authors: Wang
authors: Wen-Sen
authors: WS
authors: He
authors: Cheng-Sheng
authors: CS
authors: Jia
authors: Xiao-Ming
authors: XM
authors: Zhang
authors: Biao
authors: B
authors: Feng
authors: Da-Li
authors: DL
authors: Li
authors: Zeng
authors: Z
authors: Zeng
mesh_terms: D001547:Benzaldehydes / Q000378:metabolism*; D001711:Biotransformation; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D003653:Decarboxylation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008333:Mandelic Acids / Q000378:metabolism*; D060847:Metabolic Engineering; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001547:Benzaldehydes; D008333:Mandelic Acids; D011994:Recombinant Proteins; D002262:Carboxy-Lyases; C019778:benzoylformate decarboxylase; C037938:mandelic acid; C045941:ethyl vanillin