title: The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity.
abstract: N-Glycan from the fission yeast Schizosaccharomyces pombe contains outer-chain pyruvic acid 4,6-ketal-linked galactose (PvGal). Here, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, reported to be essential for biosynthesis of PvGal. When p-nitrophenyl-β-Gal (pNP-β-Gal) was used as a substrate, the structure of the recombinant Pvg1p product was determined to be pNP-PvGal by one- and two-dimensional NMR spectroscopy. The recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to β-linked galactose.
authors: Department of Bioscience & Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan.
authors: Ken-ichi
authors: K
authors: Yoritsune
authors: Tomohiko
authors: T
authors: Matsuzawa
authors: Takao
authors: T
authors: Ohashi
authors: Kaoru
authors: K
authors: Takegawa
mesh_terms: D019880:Aldehyde-Ketone Transferases / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002473:Cell Wall / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009154:Mutation; D010728:Phosphoenolpyruvate / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D014166:Transferases
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D029702:Schizosaccharomyces pombe Proteins; D010728:Phosphoenolpyruvate; D019289:Pyruvic Acid; D014166:Transferases; D019880:Aldehyde-Ketone Transferases; C581217:Pvg1 protein, S pombe; D005700:Galactosyltransferases; D005690:Galactose