title: Quantitative imaging of a therapeutic peptide in biological tissue sections by MALDI MS.
abstract: BACKGROUND
  Therapeutic peptides and proteins are being increasingly explored as potential therapeutic agents for molecular-targeted therapy, and the requirement for quantitative bioanalytical tools for such molecules has been discussed.
  
  
  RESULTS
  The distribution of octreotide, a synthetic octapeptide analog of somatostatin, in the liver and kidney of mice administered with the analog was clearly visualized by MALDI-Imaging MS (IMS). The developed MALDI-IMS analytical method successfully quantified the amount of octreotide on tissue sections (accuracy was 76-127%) and the 2,5-dihydroxybenzoic acid-based normalization method was effective.
  
  
  CONCLUSIONS
  The results of this study suggest that MALDI-IMS enables the quantification of an administered therapeutic peptide on biological tissue sections, as well as visualization of the in vivo distribution of the peptide.
authors: Drug Metabolism & Pharmacokinetics, Drug Developmental Research Laboratories, Shionogi & Co. Ltd., 3-1-1 Futaba, Toyonaka, Osaka 561-0825, Japan. nozomi.takai@shionogi.co.jp
authors: Nozomi
authors: N
authors: Takai
authors: Yukari
authors: Y
authors: Tanaka
authors: Ayahisa
authors: A
authors: Watanabe
authors: Hideo
authors: H
authors: Saji
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005780:Gelatin / Q000737:chemistry; D005841:Gentisates / Q000737:chemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015282:Octreotide / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005841:Gentisates; D010455:Peptides; D005780:Gelatin; D015282:Octreotide; C010925:2,5-dihydroxybenzoic acid