title: Assisted oocyte activation overcomes fertilization failure in globozoospermic patients regardless of the DPY19L2 status.
abstract: OBJECTIVE
  Does DPY19L2 status influence intracytoplasmic sperm injection (ICSI) outcomes with or without assisted oocyte activation (AOA)?
  
  
  CONCLUSIONS
  DPY19L2 mutations have no major impact on ICSI outcomes in globozoospermic patients.
  
  
  BACKGROUND
  Globozoospermia is a rare and severe teratozoospermia characterized by round-headed spermatozoa lacking an acrosome. Recently, it has been shown that DPY19L2 mutations can be found in a vast majority of, but not all, globozoospermic patients (66.7%). These patients suffer from primary infertility due to a sperm-related oocyte activation deficiency secondary to the absence of an acrosome that can be overcome by the application of AOA.
  
  
  METHODS
  Cohort study, retrospective, 34 patients, 83 cycles.
  
  
  METHODS
  Clinical and biologic data were collected from 29 patients mutated for DPY19L2 and 5 non-mutated patients. In total, 35 ICSI cycles using AOA and 48 conventional ICSI cycles were included in the analysis. Patients were divided into groups according to whether or not they were mutated for DPY19L2 and whether or not they received AOA.
  
  
  RESULTS
  Regardless of the presence of a DPY19L2 mutation, the fertilization rates with AOA are restored to normal when compared with conventional ICSI in our cohort of globozoospermic patients. Also, when performing ICSI plus AOA, both mutated and non-mutated cases have similar positive hCG rates, ongoing pregnancy rates and live birth rates per transfer. On the contrary, the fertilization rate in globozoospermic patients using conventional ICSI is correlated with the presence of a DPY19L2 mutation, with slightly better, although still very low, fertilization rates in patients carrying a DPY19L2 mutation. Nevertheless, when performing conventional ICSI, both mutated and non-mutated cases have similar very low positive hCG rates, ongoing pregnancy rates and live birth rates per transfer.
  
  
  CONCLUSIONS
  A limitation of this study is the low number of included non-mutated cases.
  
  
  CONCLUSIONS
  We propose a pathway for the clinical management of globozoospermic patients depending on the phenotype that includes several diagnostic and therapeutic steps.
  
  
  BACKGROUND
  None.
authors: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Institut National de Santé et de Recherche Médicale (INSERM) U964, Centre National de Recherche Scientifique (CNRS) UMR1704, Université de Strasbourg, 67404 Illkirch, France.
authors: P
authors: P
authors: Kuentz
authors: F
authors: F
authors: Vanden Meerschaut
authors: E
authors: E
authors: Elinati
authors: M H
authors: MH
authors: Nasr-Esfahani
authors: T
authors: T
authors: Gurgan
authors: N
authors: N
authors: Iqbal
authors: F
authors: F
authors: Carré-Pigeon
authors: F
authors: F
authors: Brugnon
authors: S A
authors: SA
authors: Gitlin
authors: J
authors: J
authors: Velez de la Calle
authors: Z
authors: Z
authors: Kilani
authors: P
authors: P
authors: De Sutter
authors: S
authors: S
authors: Viville
mesh_terms: D000177:Acrosome / Q000502:physiology; D002122:Calcium Chloride / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D018929:Cell Culture Techniques; D005260:Female; D005306:Fertilization / Q000502:physiology*; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013084:Sperm-Ovum Interactions
chemical_list: D061207:Calcium Ionophores; C511317:DPY19L2 protein, human; D008565:Membrane Proteins; D002122:Calcium Chloride