title: Fast and continuous preparation of high polymerization degree cellulose nanofibrils and their three-dimensional macroporous scaffold fabrication.
abstract: C6-carboxy-cellulose with a carboxylate content of 0.8 mmol g(-1) was obtained by oxidation of once-dried cellulose, using the 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)/NaClO/NaClO2 system at pH 6.8 and 60 °C for 16 h. This method, with the addition of reagents in the order TEMPO, NaClO and NaClO2, was 38 h faster than a previously published method. Individualized cellulose nanofibrils with a width of 3-5 nm and a length of several hundred nanometers were prepared by homogenizing the C6-carboxy-cellulose-water suspension. Macroporous cellulose nanofibril/poly(vinyl alcohol) scaffolds with interconnected large pores of 20-100 μm diameter and small pores of 2-10 μm diameter were fabricated. The cellulose nanofilaments formed nanofibrous structures on the surface of the PVA wall, which was similar to that of the collagen skeleton of the extracellular matrix. NIH/3T3 cells were cultured in the scaffolds for 4 weeks, SEM observation showed that the cells were anchored and clustered on the cellulose nanofilaments, forming spherical colonies. The extracellular matrix (ECM) was filled with mineralized particles.
authors: State Key Lab of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
authors: Jiankang
authors: J
authors: Song
authors: Aimin
authors: A
authors: Tang
authors: Tingting
authors: T
authors: Liu
authors: Jufang
authors: J
authors: Wang
mesh_terms: D000818:Animals; D002482:Cellulose / Q000737:chemistry* / Q000494:pharmacology; D002704:Chlorates / Q000737:chemistry; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D003497:Cyclic N-Oxides / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010472:Perchlorates / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D017670:Sodium Compounds / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002704:Chlorates; D003497:Cyclic N-Oxides; D010472:Perchlorates; D017670:Sodium Compounds; D011142:Polyvinyl Alcohol; D002482:Cellulose; D003094:Collagen; C031068:sodium perchlorate; C032706:sodium chlorate; C003959:TEMPO