title: Combined hemoglobin and fluorescence diffuse optical tomography for breast tumor diagnosis: a pilot study on time-domain methodology.
abstract: A combined time-domain fluorescence and hemoglobin diffuse optical tomography (DOT) system and the image reconstruction methods are proposed for enhancing the reliability of breast-dedicated optical measurement. The system equipped with two pulsed laser diodes at wavelengths of 780 nm and 830 nm that are specific to the peak excitation and emission of the FDA-approved ICG agent, and works with a 4-channel time-correlated single photon counting device to acquire the time-resolved distributions of the light re-emissions at 32 boundary sites of tissues in a tandem serial-to-parallel mode. The simultaneous reconstruction of the two optical (absorption and scattering) and two fluorescent (yield and lifetime) properties are achieved with the respective featured-data algorithms based on the generalized pulse spectrum technique. The performances of the methodology are experimentally assessed on breast-mimicking phantoms for hemoglobin- and fluorescence-DOT alone, as well as for fluorescence-guided hemoglobin-DOT. The results demonstrate the efficacy of improving the accuracy of hemoglobin-DOT based on a priori fluorescence localization.
authors: College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072,  China.
authors: Wei
authors: W
authors: Zhang
authors: Linhui
authors: L
authors: Wu
authors: Jiao
authors: J
authors: Li
authors: Xi
authors: X
authors: Yi
authors: Xin
authors: X
authors: Wang
authors: Yiming
authors: Y
authors: Lu
authors: Weiting
authors: W
authors: Chen
authors: Zhongxing
authors: Z
authors: Zhou
authors: Limin
authors: L
authors: Zhang
authors: Huijuan
authors: H
authors: Zhao
authors: Feng
authors: F
authors: Gao
keywords: (170.3010) Image reconstruction techniques; (170.3880) Medical and biological imaging; (170.6920) Time-resolved imaging; (170.6960) Tomography