title: Identification of quantitative trait loci for resistance to Verticillium wilt and yield parameters in hop (Humulus lupulus L.).
abstract: Verticillium wilt (VW) can cause substantial yield loss in hop particularly with the outbreaks of the lethal strain of Verticillium albo-atrum. To elucidate genetic control of VW resistance in hop, an F1 mapping population derived from a cross of cultivar Wye Target, with the predicted genetic basis of resistance, and susceptible male breeding line BL2/1 was developed to assess wilting symptoms and to perform QTL mapping. The genetic linkage map, constructed with 203 markers of various types using a pseudo-testcross strategy, formed ten major linkage groups (LG) of the maternal and paternal maps, covering 552.98 and 441.1 cM, respectively. A significant QTL for VW resistance was detected at LOD 7 on a single chromosomal region on LG03 of both parental maps, accounting for 24.2-26.0 % of the phenotypic variance. QTL analysis for alpha-acid content and yield parameters was also performed on this map. QTLs for these traits were also detected and confirmed our previously detected QTLs in a different pedigree and environment. The work provides the basis for exploration of QTL flanking markers for possible use in marker-assisted selection.
authors: University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors: Jernej
authors: J
authors: Jakse
authors: Andreja
authors: A
authors: Cerenak
authors: Sebastjan
authors: S
authors: Radisek
authors: Zlatko
authors: Z
authors: Satovic
authors: Zlata
authors: Z
authors: Luthar
authors: Branka
authors: B
authors: Javornik
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001947:Breeding / Q000379:methods; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D027582:Humulus / Q000235:genetics* / Q000254:growth & development* / Q000382:microbiology; D008126:Lod Score; D010935:Plant Diseases / Q000382:microbiology*; D040641:Quantitative Trait Loci / Q000235:genetics*; D020172:Verticillium
chemical_list: D017931:DNA Primers; D005819:Genetic Markers