title: SNAP-tag as a tool for surface immobilization.
abstract: SNAP-tag technology has been an important tool for protein study for more than a decade and in the meanwhile has found a number of applications beyond the field of molecular biology and protein purification. Based on covalent interaction of SNAP-tag, 20 kDA mutant of DNA repair protein and benzylguanine, it enables irreversible and controllable protein modification. In this mini review, recent developments in the use of SNAP-tag for the design of protein arrays and nanoparticle biofunctionalization are presented and discussed. A short overview of other applications that paved the way to surface modifications is also given with emphasis on fluorescent labeling through the use of SNAP-tag fusion proteins. Finally, the future of the SNAP-tag methodology for surface patterning and 3D structural scaffolding is addressed.
authors: Karlsruhe Institute of Technology (KIT), DFG-Center for Functional Nanostructures (CFN), Wolfgang Gaede Str. 1, 76131 Karlsruhe, Germany.
authors: Sinem
authors: S
authors: Engin
authors: Dagmar
authors: D
authors: Fichtner
authors: Doris
authors: D
authors: Wedlich
authors: Ljiljana
authors: L
authors: Fruk
mesh_terms: D055767:Immobilized Proteins / Q000737:chemistry*; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles; D013499:Surface Properties
chemical_list: D055767:Immobilized Proteins