title: [Development of human embryonic stem cell model for toxicity evaluation].
abstract: The current international standard for toxicity screening of biomedical devices and materials recommend the use of immortalized cell lines because of their  homogeneous morphologies and infinite proliferation which provide good reproducibility for in vitro cytotoxicity screening. However, most of the widely  used immortalized cell lines are derived from animals and may not be representative of normal human cell behavior in vivo, in particular in terms of the  cytotoxic and genotoxic response. Therefore, It is vital to develop a model for toxicity evaluation. In our studies, two Chinese human embryonic stem cell  (hESC) lines as toxicity model were established. hESC derived tissue/organ cell model for tissue/organ specific toxicity evaluation were developed. The  efficiency and accuracy of using hESC model for cytoxicity, embryotoxicity and genotoxicity evaluation were confirmed. The results indicated that hESCs  might be good tools for toxicity testing and biosafety evaluation in vitro.
authors: Peking University School and Hospital of Stomatology, Beijing, China.
authors: Guang-yan
authors: GY
authors: Yu
authors: Tong
authors: T
authors: Cao
authors: Hong-wei
authors: HW
authors: Ouyang
authors: Shuang-qing
authors: SQ
authors: Peng
authors: Xu-liang
authors: XL
authors: Deng
authors: Sheng-lin
authors: SL
authors: Li
authors: He
authors: H
authors: Liu
authors: Xiao-hui
authors: XH
authors: Zou
authors: Xin
authors: X
authors: Fu
authors: Hui
authors: H
authors: Peng
authors: Xiao-ying
authors: XY
authors: Wang
authors: Yuan
authors: Y
authors: Zhan
mesh_terms: D044466:Asian People; D018929:Cell Culture Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D018675:Toxicity Tests / Q000379:methods*