title: Venezuelan equine encephalitis virus replicon particle vaccine protects nonhuman primates from intramuscular and aerosol challenge with ebolavirus.
abstract: There are no vaccines or therapeutics currently approved for the prevention or treatment of ebolavirus infection. Previously, a replicon vaccine based on Venezuelan equine encephalitis virus (VEEV) demonstrated protective efficacy against Marburg virus in nonhuman primates. Here, we report the protective efficacy of Sudan virus (SUDV)- and Ebola virus (EBOV)-specific VEEV replicon particle (VRP) vaccines in nonhuman primates. VRP vaccines were developed to express the glycoprotein (GP) of either SUDV or EBOV. A single intramuscular vaccination of cynomolgus macaques with VRP expressing SUDV GP provided complete protection against intramuscular challenge with SUDV. Vaccination against SUDV and subsequent survival of SUDV challenge did not fully protect cynomolgus macaques against intramuscular EBOV back-challenge. However, a single simultaneous intramuscular vaccination with VRP expressing SUDV GP combined with VRP expressing EBOV GP did provide complete protection against intramuscular challenge with either SUDV or EBOV in cynomolgus macaques. Finally, intramuscular vaccination with VRP expressing SUDV GP completely protected cynomolgus macaques when challenged with aerosolized SUDV, although complete protection against aerosol challenge required two vaccinations with this vaccine.
authors: U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA.
authors: Andrew S
authors: AS
authors: Herbert
authors: Ana I
authors: AI
authors: Kuehne
authors: James F
authors: JF
authors: Barth
authors: Ramon A
authors: RA
authors: Ortiz
authors: Donald K
authors: DK
authors: Nichols
authors: Samantha E
authors: SE
authors: Zak
authors: Spencer W
authors: SW
authors: Stonier
authors: Majidat A
authors: MA
authors: Muhammad
authors: Russell R
authors: RR
authors: Bakken
authors: Laura I
authors: LI
authors: Prugar
authors: Gene G
authors: GG
authors: Olinger
authors: Jennifer L
authors: JL
authors: Groebner
authors: John S
authors: JS
authors: Lee
authors: William D
authors: WD
authors: Pratt
authors: Max
authors: M
authors: Custer
authors: Kurt I
authors: KI
authors: Kamrud
authors: Jonathan F
authors: JF
authors: Smith
authors: Mary Kate
authors: MK
authors: Hart
authors: John M
authors: JM
authors: Dye
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D029043:Ebolavirus / Q000235:genetics / Q000276:immunology*; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors / Q000235:genetics / Q000502:physiology; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D008252:Macaca fascicularis; D012093:Replicon; D014611:Vaccination; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines