title: Oncolytic vaccinia virus therapy of salivary gland carcinoma.
abstract: OBJECTIVE
  To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel of 5 human salivary gland carcinoma cell lines.
  
  
  METHODS
  The susceptibility of 5 salivary gland carcinoma cell lines to infection and oncolysis by GLV-1h68 was assessed in vitro and in vivo.
  
  
  RESULTS
  All 5 cell lines were susceptible to viral infection, transgene expression, and cytotoxic reactions. Three cell lines were exquisitely sensitive to infection by very low doses of GLV-1h68. Orthotopic parotid tumors exhibited more aggressive behavior compared with flank tumors. A single intratumoral injection of GLV-1h68 induced significant tumor regression without observed toxic effects in flank and parotid tumor models; controls demonstrated rapid tumor progression.
  
  
  CONCLUSIONS
  These promising results demonstrate significant oncolytic activity by an attenuated vaccinia virus for infecting and lysing salivary gland carcinomas, supporting future clinical trials.
authors: Departments of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
authors: Natalya
authors: N
authors: Chernichenko
authors: Gary
authors: G
authors: Linkov
authors: Pingdong
authors: P
authors: Li
authors: Richard L
authors: RL
authors: Bakst
authors: Chun-Hao
authors: CH
authors: Chen
authors: Shuangba
authors: S
authors: He
authors: Yong A
authors: YA
authors: Yu
authors: Nanhai G
authors: NG
authors: Chen
authors: Aladar A
authors: AA
authors: Szalay
authors: Yuman
authors: Y
authors: Fong
authors: Richard J
authors: RJ
authors: Wong
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000628:therapy*; D000818:Animals; D018277:Carcinoma, Mucoepidermoid / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D007267:Injections; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D050130:Oncolytic Virotherapy; D012468:Salivary Gland Neoplasms / Q000473:pathology / Q000628:therapy*; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication; D023041:Xenograft Model Antitumor Assays; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D008156:Luciferases; D001616:beta-Galactosidase