title: Antioxidant activity of ethanolic fractions of Polish propolis.
abstract: There is a great variation in the chemical composition of propolis of different origins. Likewise, the method of its extraction has significant impact on the content of biologically active compounds. Here we compared methods of propolis extraction for optimal antioxidant activities which were measured by means of beta-carotene discolouration, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS*+) radical cation decolouration assays. In the extracts, the contents of polyphenols and flavonoids were measured, and phenolic acids were identified and quantified by HPLC. A three-step extraction allowed obtaining large amounts of phenolic acids from propolis. The propolis fractions obtained had antioxidant properties comparable to those of alpha-tocopherol and butylated hydroxytoluene. Therefore, they may be used as effective natural antioxidants.
authors: Department of Food and Nutrition, Medical University of Silesia, The School of Pharmacy and Division of Laboratory Medicine, Jedności 8, 41-200 Sosnowiec, Poland. katedrazywnosci@sum.edu.pl
authors: Anna M
authors: AM
authors: Kurek-Górecka
authors: Andrzej
authors: A
authors: Sobczakb
authors: Anna
authors: A
authors: Rzepecka-Stojkod
authors: Michał T
authors: MT
authors: Górecki
authors: Maria
authors: M
authors: Wardas
authors: Katarzyna
authors: K
authors: Pawłowska-Góral
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D000431:Ethanol; D011429:Propolis