title: VEGF receptor-1 involvement in pericyte loss induced by Escherichia coli in an in vitro model of blood brain barrier.
abstract: The key aspect of neonatal meningitis is related to the ability of pathogens to invade the blood-brain barrier (BBB) and to penetrate the central nervous system. In the present study we show that, in an in vitro model of BBB, on the basis of co-culturing primary bovine brain endothelial cells (BBEC) and primary bovine retinal pericytes (BRPC), Escherichia coli infection determines changes of transendothelial electrical resistance (TEER) and permeability (Pe) to sodium fluorescein. In the co-culture model, within BBEC, bacteria are able to stimulate cytosolic and Ca(2+)-independent phospholipase A2 (cPLA2 and iPLA2 ) enzyme activities. In supernatants of E. coli-stimulated co-cultures, an increase in prostaglandins (PGE2) and VEGF production in comparison with untreated co-cultures were found. Incubation with E. coli in presence of AACOCF3 or BEL caused a decrease of PGE2 and VEGF release. SEM and TEM images of BBEC and BRPC showed E. coli adhesion to BBEC and BRPC but only in BBEC the invasion occurs. VEGFR-1 but not VEGFR-2 blockade by the specific antibody reduced E. coli invasion in BBEC. In our model of BBB infection, a significant loss of BRPC was observed. Following VEGFR-1, but not VEGFR-2 blockade, or in presence of AACOCF3 or BEL, elevated TEER values, reduced permeability and BRPC loss were found. These data suggest that VEGFR-1 negatively regulates BRPC survival and its blockade protects the barrier integrity. PGs and VEGF could exert a biological effect on BBB, probably by BRPC coverage ablation, thus increasing BBB permeability. Our results show the role played by the BBEC as well as BRPC during a bacterial attack on BBB. A better understanding of the mechanisms by which E. coli enter the nervous system and how bacteria alter the communication between endothelial cells and pericytes may provide exciting new insight for clinical intervention.
authors: Department of Bio-medical Sciences, University of Catania, Catania, Italy.
authors: Mario
authors: M
authors: Salmeri
authors: Carla
authors: C
authors: Motta
authors: Carmelina D
authors: CD
authors: Anfuso
authors: Andrea
authors: A
authors: Amodeo
authors: Marina
authors: M
authors: Scalia
authors: Maria A
authors: MA
authors: Toscano
authors: Mario
authors: M
authors: Alberghina
authors: Gabriella
authors: G
authors: Lupo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000502:physiology; D001812:Blood-Brain Barrier / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology*; D002417:Cattle; D002463:Cell Membrane Permeability / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D017097:Electric Impedance; D004730:Endothelium, Vascular / Q000382:microbiology / Q000473:pathology*; D004926:Escherichia coli / Q000472:pathogenicity* / Q000502:physiology; D004927:Escherichia coli Infections / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D019793:Fluorescein; D005456:Fluorescent Dyes; D020286:Pericytes / Q000382:microbiology / Q000473:pathology*; D054467:Phospholipases A2 / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000187:drug effects / Q000276:immunology / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D040281:Vascular Endothelial Growth Factor Receptor-1; D054467:Phospholipases A2; D015232:Dinoprostone; D019793:Fluorescein