title: [Effect of aluminum trichloride on abnormal phosphorylation of tau protein in SH-SY5Y cells].
abstract: OBJECTIVE
  To investigate the effect of aluminum trichloride on the abnormal phosphorylation of tau protein in SH-SY5Y cells.
  
  
  METHODS
  SH-SY5Y cells were assigned to control group and aluminum trichloride exposure groups (200, 400, and 800 µmol/L Al(3+)). The cell morphology was observed after 48 hours of exposure; the cell viability was measured by CCK-8 assay; total protein was extracted from the cells, and the expression of phospho-tau (p-tau) 181, 231, 262, and 396 and tau 5 was measured by Western blot.
  
  
  RESULTS
  As the Al(3+) concentration rose, the number of living SH-SY5Y cells decreased, and the synapses of the cells retracted. The viability of cells exposed to 800 µmol/L Al(3+) was significantly lower than that of the control group (P < 0.05). The 200, 400, and 800 µmol/L Al(3+) exposure groups showed significantly higher expression of p-tau 181, 231, and 396 and tau5 than the control group (P < 0.05), and the 800 µmol/L Al(3+) exposure group showed significantly higher expression of p-tau 262 than the control group (P < 0.05).
  
  
  CONCLUSIONS
  Under the present experimental conditions, aluminum trichloride has toxic effect on SH-SY5Y cells and can lead to abnormal expression of p-tau 181, 231, and 396 and tau 5 at low Al(3+) concentration.
authors: Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Hao
authors: H
authors: Wang
authors: Xiao-ting
authors: XT
authors: Lu
authors: Zhi-jian
authors: ZJ
authors: Jia
authors: Qiao
authors: Q
authors: Niu
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002712:Chlorides / Q000633:toxicity*; D006801:Humans; D010766:Phosphorylation; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D016875:tau Proteins; D000077410:Aluminum Chloride