title: The group 10 allergen of Dermatophagoides farinae (Acari: Pyroglyphidae): cDNA cloning, sequence analysis, and expression in Escherichia coli BL21.
abstract: Dermatophagoides farinae Hughes, American house dust mite, is highly allergenic, producing symptoms in people worldwide. Identifying and cloning the allergens in this species may enable better diagnostic and therapeutic approaches. Here, we cloned, sequenced, and expressed the full-length cDNA encoding D. farinae group 10 allergen (Der f 10) isolated from dust mites in China. Bioinformatic analysis indicated that the 888 bp sequence encoded a cytoskeleton protein 295 amino acids long, with a molecular weight of approximately equal 34 kDa. Sequence alignment with the group 10 allergens of Pyroglyphidae, Acaridae, and Glycyphagidae families revealed that the group 10 allergen from D. farinae is 95% similar to D. pteronyssinus Trouessart and Psoroptes ovis (Hering). These findings lay the groundwork for future studies, including large-scale production of recombinant Der f 10 allergen for diagnostic and therapeutic agents.
authors: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Jiefangnan Road 263, Yancheng 224006, Jiangsu Yancheng 224006, P. R. China. ybcui1975@hotmail.com
authors: Yubao
authors: Y
authors: Cui
authors: Ying
authors: Y
authors: Zhou
authors: Yungang
authors: Y
authors: Wang
authors: Guifang
authors: G
authors: Ma
authors: Li
authors: L
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D040001:Dermatophagoides farinae / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins; D017422:Sequence Analysis, DNA
chemical_list: D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D011994:Recombinant Proteins