title: Dissecting glycosylation steps in lobophorin biosynthesis implies an iterative glycosyltransferase.
abstract: The identification of a lobophorin biosynthetic gene cluster from the deep-sea derived Streptomyces sp. SCSIO 01127 reveals a paradigm of three glycosyltransferases (GTs) LobG1-LobG3 being responsible for appending four sugars. Characterization of five differentially glycosylated metabolites from three GT gene-inactivation mutants allowed the assignment of GT functions and the implication of LobG3 as an iterative GT to attach two digitoxoses.
authors: CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
authors: Sumei
authors: S
authors: Li
authors: Ji
authors: J
authors: Xiao
authors: Yiguang
authors: Y
authors: Zhu
authors: Guangtao
authors: G
authors: Zhang
authors: Chunfang
authors: C
authors: Yang
authors: Haibo
authors: H
authors: Zhang
authors: Liang
authors: L
authors: Ma
authors: Changsheng
authors: C
authors: Zhang
mesh_terms: D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D006601:Hexoses / Q000737:chemistry; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D005810:Multigene Family; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics
chemical_list: D006601:Hexoses; D018942:Macrolides; C121297:lobophorin A; C020615:digitoxose; D016695:Glycosyltransferases