title: Myeloperoxidase activity and its corresponding mRNA expression as well as gene polymorphism in the population living in the coal-burning endemic fluorosis area in Guizhou of China.
abstract: The myeloperoxidase (MPO) activity and its corresponding mRNA expression as well as gene polymorphism were investigated in the population who live in the endemic fluorosis area. In the study, 150 people were selected from the coal-burning endemic fluorosis area and 150 normal persons from the non-fluorosis area in Guizhou province of China. The blood samples were collected from these people. The activity of MPO in the plasma was determined by spectrophotometer; the expression of MPO mRNA was measured by employing real-time polymerase chain reaction; DNAs were extracted from the leucocytes in blood and five SNP genotypes of MPO promoter gene detected by a multiplex genotyping method, adapter-ligation-mediated allele-specific amplification. The results showed that the MPO activity and its corresponding mRNA in blood were significantly increased in the population living in the area of fluorosis. The different genotype frequencies of MPO, including -1228G/A, -585T/C, -463G/A, and -163C/T, and the three haplotypes with higher frequencies, including -163C-463G-585T-1228G-1276T, -163C-463G-585T-1228G-1276C, and -163C-463G-585T-1228A-1276T, were significantly associated with fluorosis. The results indicated that the elevated activity of MPO induced by endemic fluorosis may be connected in mechanism to the stimulated expression of MPO mRNA and the changed gene polymorphism.
authors: Key Laboratory of Molecular Biology, Guiyang Medical College, Guiyang, People's Republic of China.
authors: Ting
authors: T
authors: Zhang
authors: Ke-Ren
authors: KR
authors: Shan
authors: Xi
authors: X
authors: Tu
authors: Yan
authors: Y
authors: He
authors: Jin-Jing
authors: JJ
authors: Pei
authors: Zhi-Zhong
authors: ZZ
authors: Guan
mesh_terms: D000393:Air Pollutants / Q000633:toxicity; D002681:China / Q000453:epidemiology; D003031:Coal; D019353:Endemic Diseases; D005461:Fluorine / Q000097:blood / Q000633:toxicity*; D009050:Fluorosis, Dental / Q000201:enzymology / Q000453:epidemiology / Q000209:etiology* / Q000235:genetics; D005506:Food Contamination / Q000032:analysis; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D009195:Peroxidase / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000393:Air Pollutants; D003031:Coal; D012333:RNA, Messenger; D005461:Fluorine; D009195:Peroxidase