title: Influence of poly(n-isopropylacrylamide)-CNT-polyaniline three-dimensional electrospun microfabric scaffolds on cell growth and viability.
abstract: This study investigates the effect on: (1) the bulk surface and (2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacrylamide)-CNT-polyaniline on growth and viability of cells. The poly(N-isopropylacrylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non-woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 μm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacrylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacrylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N-isopropylacrylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N-isopropylacrylamide). The results suggest that poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering.
authors: Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Linköping University, S-58183 Linköping, Sweden; International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan. ashutosh.tiwari@liu.se.
authors: Ashutosh
authors: A
authors: Tiwari
authors: Yashpal
authors: Y
authors: Sharma
authors: Shinya
authors: S
authors: Hattori
authors: Dohiko
authors: D
authors: Terada
authors: Ashok K
authors: AK
authors: Sharma
authors: Anthony P F
authors: AP
authors: Turner
authors: Hisatoshi
authors: H
authors: Kobayashi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival; D003094:Collagen; D004338:Drug Combinations; D055664:Electrochemical Techniques / Q000379:methods; D005347:Fibroblasts / Q000166:cytology; D007739:L Cells; D007797:Laminin; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D011509:Proteoglycans; D013696:Temperature; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D000814:Aniline Compounds; D004338:Drug Combinations; D007797:Laminin; D011108:Polymers; D011509:Proteoglycans; C416807:polyaniline; C059485:matrigel; C052970:poly-N-isopropylacrylamide; D003094:Collagen