title: Gene transfer and mutagenesis mediated by Sleeping Beauty transposon in Nile tilapia (Oreochromis niloticus).
abstract: The success of gene transfer has been demonstrated in many of vertebrate species, whereas the efficiency of producing transgenic animals remains pretty low due to the random integration of foreign genes into a recipient genome. The Sleeping Beauty (SB) transposon is able to improve the efficiency of gene transfer in zebrafish and mouse, but its activity in tilapia (Oreochromis niloticus) has yet to be characterized. Herein, we demonstrate the potential of using the SB transposon system as an effective tool for gene transfer and insertional mutagenesis in tilapia. A transgenic construct pT2/tiHsp70-SB11 was generated by subcloning the promoter of tilapia heat shock protein 70 (tiHsp70) gene, the SB11 transposase gene and the carp β-actin gene polyadenylation signal into the second generation of SB transposon. Transgenic tilapia was produced by microinjection of this construct with in vitro synthesized capped SB11 mRNA. SB11 transposon was detected in 28.89 % of founders, 12.9 % of F1 and 43.75 % of F2. Analysis of genomic sequences flanking integrated transposons indicates that this transgenic tilapia line carries two copies of SB transposon, which landed into two different endogenous genes. Induced expression of SB11 gene after heat shock was detected using reverse transcription PCR in F2 transgenic individuals. In addition, the Cre/loxP system was introduced to delete the SB11 cassette for stabilization of gene interruption and bio-safety. These findings suggest that the SB transposon system is active and can be used for efficient gene transfer and insertional mutagenesis in tilapia.
authors: The Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, 7 Donghu Rd, Wuhan, Hubei, 430072, People's Republic of China.
authors: Xiaozhen
authors: X
authors: He
authors: Jie
authors: J
authors: Li
authors: Yong
authors: Y
authors: Long
authors: Guili
authors: G
authors: Song
authors: Peiyong
authors: P
authors: Zhou
authors: Qiuxiang
authors: Q
authors: Liu
authors: Zuoyan
authors: Z
authors: Zhu
authors: Zongbin
authors: Z
authors: Cui
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D023681:Cichlids / Q000235:genetics*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D018014:Gene Transfer Techniques; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D007315:Insemination, Artificial; D008845:Microinjections; D016254:Mutagenesis, Insertional / Q000235:genetics*; D019895:Transposases / Q000235:genetics
chemical_list: D000199:Actins; D004251:DNA Transposable Elements; D018840:HSP70 Heat-Shock Proteins; D019895:Transposases