title: SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe⁺ implantation.
abstract: Ion implantation,  a new biophysically mutagenic technique, has shown great potential for crop  breeding. To reveal the mutation effect of low-energy ion implantation on  Baiyangdian red lotus, sequence-related amplified polymorphism markers were  used to amplify and detect the DNA sequence differences in mutants induced by  Fe(+) ion  implantation. A total of 121 primer combinations were tested in 6  mutants and a control. Seven primer combinations (me1 + em3, me1 + em14, me9 +  em3, me8 + em2, me6 + em1, me11 + em5, and me6 + em5) generated clear bands  with high polymorphism and good repeatability. The results showed that among  15,317 bases cloned, 146 bases in 6 mutants were different from those of the  wild type, showing a variation frequency of 0.95%. The types of base changes  included deletion, insertion, transversion, and transition. Adenine was more  sensitive to the irradiation than were the other bases. The results suggested  that mutational "hotspots" probably exists in lotus and are induced by  low-energy ion implantation.
authors: College of Life Science, Henan Normal University, Xinxiang, China. dcl75@yahoo.cn
authors: C L
authors: CL
authors: Deng
authors: R Y
authors: RY
authors: Qin
authors: J
authors: J
authors: Gao
authors: Y Y
authors: YY
authors: Jia
authors: Y X
authors: YX
authors: Ren
authors: W J
authors: WJ
authors: Gao
authors: L D
authors: LD
authors: Lu
mesh_terms: D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D007477:Ions / Q000737:chemistry; D058085:Iron Compounds / Q000737:chemistry*; D000070116:Lotus / Q000235:genetics*; D009154:Mutation; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D007477:Ions; D058085:Iron Compounds