title: [Experimental study on delta-tocotrienol inhibits the Wnt pathway in the colon cancer cell SW620].
abstract: OBJECTIVE
  Based on the antitumor function of the delta-tocotrienol, shed light on the relationship between the proliferation inhibition effect of delta-tocotrienol on human colon cancer cells SW620 with Wnt signal pathway.
  
  
  METHODS
  To adopt the MTT method, found the effects of diverse doses of delta-tocotrienol on colon cancer cells SW620. Detected the expressions level of the factors related to Wnt signal pathway via Western bloting and immunocytochemistry, to observe the influence of delta-tocotrienol on Wnt-1, beta-catenin, c-jun, cylin D1.
  
  
  RESULTS
  delta-tocotrienol has a significant proliferation inhibition effect on the colon cancer cells SW620,under delta-tocotrienol treated, SW620 cell inhibition rate is 70.43%. The IC50 is 15.18 micromol/L. The expression levels of wnt-1, beta-catenin c-jun and cylin D1 are downregulated (P < 0.05).
  
  
  CONCLUSIONS
  The proliferation inhibition effect of delta-tocotrienol on colon cancer cell may correlate with the ability of delta-tocotrienol to downregulate the expression levels of wnt-1, beta-catenin, c-jun, cylin D1 in the Wnt signal pathway.
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China. xiaoma3477@sina.cn
authors: M A
authors: MA
authors: Yue
authors: Ning
authors: N
authors: He
authors: Jing
authors: J
authors: Zhang
authors: Daming
authors: D
authors: Li
authors: Yinghua
authors: Y
authors: Liu
authors: Jingshu
authors: J
authors: Zhang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D019938:Cyclin D1 / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051155:Wnt1 Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C530104:CCND1 protein, human; C495270:CTNNB1 protein, human; D016755:Proto-Oncogene Proteins c-jun; C495247:WNT1 protein, human; D051155:Wnt1 Protein; D051176:beta Catenin; D019938:Cyclin D1; D014810:Vitamin E; C082097:tocotrienol, delta