title: Reprogramming the phenylpropanoid metabolism in seeds of oilseed rape by suppressing the orthologs of reduced epidermal fluorescence1.
abstract: As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene reduced epidermal fluorescence1 (REF1) encoding a coniferaldehyde/sinapaldehyde dehydrogenase. The enzyme is involved in the formation of ferulate and sinapate from the corresponding aldehydes, thereby linking lignin and hydroxycinnamate biosynthesis as a potential branch-point enzyme. We used RNA interference to silence REF1 genes in seeds of oilseed rape. Nontargeted metabolite profiling showed that BnREF1-suppressing seeds produced a novel chemotype characterized by reduced levels of sinapate esters, the appearance of conjugated monolignols, dilignols, and trilignols, altered accumulation patterns of kaempferol glycosides, and changes in minor conjugates of caffeate, ferulate, and 5-hydroxyferulate. BnREF1 suppression affected the level of minor sinapate conjugates more severely than that of the major component sinapine. Mapping of the changed metabolites onto the phenylpropanoid metabolic network revealed partial redirection of metabolic sequences as a major impact of BnREF1 suppression.
authors: Department of Secondary Metabolism, Leibniz Institute of Plant Biochemistry, D-06120 Halle, Germany.
authors: Juliane
authors: J
authors: Mittasch
authors: Christoph
authors: C
authors: Böttcher
authors: Andrej
authors: A
authors: Frolov
authors: Dieter
authors: D
authors: Strack
authors: Carsten
authors: C
authors: Milkowski
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry*; D053898:Biosynthetic Pathways; D015139:Blotting, Southern; D029688:Brassica napus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002794:Choline / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003433:Crosses, Genetic; D004952:Esters / Q000737:chemistry / Q000378:metabolism; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D006720:Homozygote; D055442:Metabolome; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020005:Propanols / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D004952:Esters; D010940:Plant Proteins; D020005:Propanols; C005685:sinapine; C439395:1-phenylpropanol; D000444:Aldehyde Dehydrogenase; C487188:reduced epidermal fluorescence1 protein, Arabidopsis; D002794:Choline