title: Structure of DNA coils in dilute and semidilute solutions.
abstract: We apply scanning fluorescence correlation spectroscopy to study the structure of individual DNA coils in dilute and semidilute solutions. In dilute solutions, over two decades in length, from 0.6 to 46 μm, DNA behave as ideal chains, in agreement with theoretical predictions and in disagreement with prior experiments. In semidilute solutions, up to very high densities, the structures of individual DNA coils are independent of concentration, unlike flexible coils that shrink with increasing density. Our experimental findings are consistent with the marginal solution theory of semiflexible polymers.
authors: Physics Department, Ben-Gurion University, Beer-Sheva 84105, Israel.
authors: Manish
authors: M
authors: Nepal
authors: Alon
authors: A
authors: Yaniv
authors: Eyal
authors: E
authors: Shafran
authors: Oleg
authors: O
authors: Krichevsky
mesh_terms: D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D012996:Solutions; D004247:DNA; D004996:Ethidium