title: Expression of annexin-A1 and galectin-1 anti-inflammatory proteins and mRNA in chronic gastritis and gastric cancer.
abstract: OBJECTIVE
  The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection.
  
  
  METHODS
  We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay.
  
  
  RESULTS
  High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26  ±  2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38  ±  4.77). However, LGALS1 mRNA levels were high (mean RQ = 2.44  ±  3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43 ± 3.13; P < 0.01). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins.
  
  
  CONCLUSIONS
  These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis.
authors: Department of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors: Yvana Cristina
authors: YC
authors: Jorge
authors: Mayra Mioto
authors: MM
authors: Mataruco
authors: Leandro Pires
authors: LP
authors: Araújo
authors: Ana Flávia Teixeira
authors: AF
authors: Rossi
authors: Juliana Garcia
authors: JG
authors: de Oliveira
authors: Marina Curado
authors: MC
authors: Valsechi
authors: Alaor
authors: A
authors: Caetano
authors: Kenji
authors: K
authors: Miyazaki
authors: Célia Sebastiana de Jesus
authors: CS
authors: Fazzio
authors: Jorge Alberto
authors: JA
authors: Thomé
authors: Paula
authors: P
authors: Rahal
authors: Sonia Maria
authors: SM
authors: Oliani
authors: Ana Elizabete
authors: AE
authors: Silva
mesh_terms: D000328:Adult; D000368:Aged; D017305:Annexin A1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism*; D005756:Gastritis / Q000235:genetics / Q000378:metabolism*; D016481:Helicobacter Infections / Q000503:physiopathology; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D017305:Annexin A1; D037483:Galectin 1; D012333:RNA, Messenger