title: Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation.
abstract: We report intron chromosomal expression FISH (iceFISH), a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We find substantial differences in transcriptional frequency between genes on a translocated chromosome and the same genes in their normal chromosomal context in the same cell. Correlations between genes on a single chromosome pointed toward a cis chromosome-level transcriptional interaction spanning 14.3 megabases.
authors: Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors: Marshall J
authors: MJ
authors: Levesque
authors: Arjun
authors: A
authors: Raj
mesh_terms: D018929:Cell Culture Techniques; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D004247:DNA / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics*; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014178:Translocation, Genetic
chemical_list: D012333:RNA, Messenger; D004247:DNA