title: Geodermatophilus tzadiensis sp. nov., a UV radiation-resistant bacterium isolated from sand of the Saharan desert.
abstract: Three novel Gram-positive, aerobic, actinobacterial strains, CF5/2(T), CF5/1 and CF7/1, were isolated in 2007 during environmental screening of arid desert soil in the Sahara desert, Chad. Results from riboprinting, MALDI-TOF protein spectra and 16S rRNA sequence analysis confirmed that all three strains belonged to the same species. Phylogenetic analysis of 16S rRNA sequences with the strains' closest relatives indicated that they represented a distinct species. The three novel strains also shared a number of physiological and biochemical characteristics distinct from previously named Geodermatophilus species. The novel strains' peptidoglycan contained meso-diaminopimelic acid; their main phospholipids were phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and a small amount of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone. The major cellular fatty acids were the branched-chain saturated acids iso-C16:0 and iso-C15:0. Galactose was detected as diagnostic sugar. Based on these chemotaxonomic results, 16S rRNA gene sequence analysis and DNA-DNA hybridization between strain CF5/2(T) and the type strains of Geodermatophilus saharensis, Geodermatophilus arenarius, Geodermatophilus nigrescens, Geodermatophilus telluris and Geodermatophilus siccatus, the isolates CF5/2(T), CF5/1 and CF7/1 are proposed to represent a novel species, Geodermatophilus tzadiensis, with type strain CF5/2(T)=DSM 45416=MTCC 11411 and two reference strains, CF5/1 (DSM 45415) and CF7/1 (DSM 45420).
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124 Braunschweig, Germany.
authors: Maria del Carmen
authors: Mdel C
authors: Montero-Calasanz
authors: Markus
authors: M
authors: Göker
authors: William J
authors: WJ
authors: Broughton
authors: Arlette
authors: A
authors: Cattaneo
authors: Jocelyne
authors: J
authors: Favet
authors: Gabriele
authors: G
authors: Pötter
authors: Manfred
authors: M
authors: Rohde
authors: Cathrin
authors: C
authors: Spröer
authors: Peter
authors: P
authors: Schumann
authors: Hans-Peter
authors: HP
authors: Klenk
authors: Anna A
authors: AA
authors: Gorbushina
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects* / Q000648:ultrastructure; D000352:Africa, Northern; D015373:Bacterial Typing Techniques; D003889:Desert Climate; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011836:Radiation Tolerance; D012822:Silicon Dioxide; D014466:Ultraviolet Rays
chemical_list: D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide