title: Differentiation of bovine spermatogonial stem cells into osteoblasts.
abstract: Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show their multipotentiality. In the present study, bovine SSCs were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were identified by Vimentin and Oct-4 immunocytochemical staining method, respectively. In order to differentiate SSCs into osteoblasts, we used consecutive inducer media without separation of the colonies. We characterized osteoblasts using Alizarin red staining.
authors: Department of Clinical Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Babak
authors: B
authors: Qasemi-Panahi
authors: Parviz
authors: P
authors: Tajik
authors: Mansoureh
authors: M
authors: Movahedin
authors: Gholamali
authors: G
authors: Moghaddam
authors: Younes
authors: Y
authors: Barzgar
authors: Hamed
authors: H
authors: Heidari-Vala
keywords: Differentiation; Osteoblast; Spermatogonial stem cell