title: Unique antigenic gene expression at different developmental stages of Trichinella pseudospiralis.
abstract: Parasite-induced and parasite-regulated larval capsule formation and host immunosuppression are two major characteristics that are unique in Trichinella spp. infections, but the molecule(s) and mechanism(s) that mediate these processes remain largely unknown. Trichinella pseudospiralis and Trichinella spiralis, are obviously different with respect to these two characteristics. A comparative study of these two species, in particular their antigen expression profiles at different developmental stages (the main molecules involved in the cross-talk or interaction between each parasite and its host), may help us better understand the parasite molecules and mechanisms involved. Here, we constructed cDNA libraries from T. pseudospiralis adults (Ad), newborn larvae (NBL) and muscle larvae (ML) mRNA and screened them with pig anti-T. pseudospiralis serum collected 26, 32 and 60 days post-infection (p.i.). The most abundant antigens were found to vary among life-cycle stages. Pyroglutamy peptidase 1-like and 6-phosphogluconolactonase-like genes predominated in the Ad stage and a serine protease (SS2-1-like gene) predominated in NBL similar to that observed in T. spiralis. Muscle larvae expressed proteasome activator complex subunit 3-like and 21 kDa excretory/secretory protein-like genes. This study indicated that parasites of two species may utilise different molecules and mechanisms for larvae capsule formation and host immunosuppression during their infections. Proteins of antigenic genes identified in this study may be also good candidates for diagnosis, treatment or vaccination for T. pseudospiralis infection, and also for the differential diagnosis of two species' infections.
authors: Key Laboratory for Zoonosis Research, Ministry of Education, Jilin University, Chinese Center for Disease Control and Prevention, China.
authors: X P
authors: XP
authors: Wu
authors: X L
authors: XL
authors: Liu
authors: X L
authors: XL
authors: Wang
authors: R
authors: R
authors: Blaga
authors: B Q
authors: BQ
authors: Fu
authors: P
authors: P
authors: Liu
authors: X
authors: X
authors: Bai
authors: Z J
authors: ZJ
authors: Wang
authors: B M
authors: BM
authors: Rosenthal
authors: H N
authors: HN
authors: Shi
authors: L
authors: L
authors: Sandrine
authors: I
authors: I
authors: Vallee
authors: P
authors: P
authors: Boireau
authors: F
authors: F
authors: Wang
authors: X N
authors: XN
authors: Zhou
authors: Y
authors: Y
authors: Zhao
authors: M Y
authors: MY
authors: Liu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000378:metabolism; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva; D008018:Life Cycle Stages / Q000235:genetics*; D051379:Mice; D009132:Muscles / Q000469:parasitology; D018510:RNA, Helminth / Q000235:genetics; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D014234:Trichinella / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology*
chemical_list: D000947:Antigens, Helminth; D018509:DNA, Helminth; D015801:Helminth Proteins; D018510:RNA, Helminth