title: Characterization of vitamin B₁₂compounds in the wild edible mushrooms black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius).
abstract: This study determined the vitamin B₁₂ content of six wild edible mushrooms which are consumed by European vegetarians. Zero or trace levels (0.01-0.09 µg/100 g dry weight) of vitamin B₁₂ were determined in porcini mushrooms (Boletus spp.), parasol mushrooms (Macrolepiota procera), oyster mushrooms (Pleurotus ostreatus), and black morels (Morchella conica). By contrast, black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius) mushrooms contained considerable levels (1.09-2.65 µg/100 g dry weight) of vitamin B₁₂. To determine whether C. cornucopioides or C. cibarius contained vitamin B₁₂ or other corrinoid compounds that are inactive in humans, we purified a corrinoid compound using an immunoaffinity column and identified it as vitamin B₁₂ based on LC/ESI-MS/MS chromatograms.
authors: School of Agricultural, Biological and Environmental Sciences, Tottori University, Tottori, Japan. watanabe@muses.tottori-u.ac.jp
authors: Fumio
authors: F
authors: Watanabe
authors: Joachi
authors: J
authors: Schwarz
authors: Shigeo
authors: S
authors: Takenaka
authors: Emi
authors: E
authors: Miyamoto
authors: Noriharu
authors: N
authors: Ohishi
authors: Esther
authors: E
authors: Nelle
authors: Rahel
authors: R
authors: Hochstrasser
authors: Yukinori
authors: Y
authors: Yabuta
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000145:classification; D002853:Chromatography, Liquid; D053719:Tandem Mass Spectrometry; D014805:Vitamin B 12 / Q000032:analysis*
chemical_list: D014805:Vitamin B 12