title: [In vitro interferon gamma production in mice vaccinated with 'Mycobacterium habana' against Mycobacterium tuberculosis antigens].
abstract: BACKGROUND
  development of new antituberculosis vaccines requires the characterization of the cell-mediated immune responses induced by mycobacterial antigens.
  
  
  OBJECTIVE
  to determine the immunogenic potential of 'Mycobacterium habana' TMC-5135 when using subcutaneous vaccine in Balb/c mice.
  
  
  METHODS
  in this study, Balb/c mice were inoculated subcutaneously with live 'Mycobacterium habana' TMC-5135. The production of IFN gamma in cell suspensions obtained from the lungs, the spleen and the lymph nodes after stimulation with mycobacterial antigens Ag85b or culture filtrate antigens (CFA) was recorded.
  
  
  RESULTS
  the production of IFN gamma after stimulation with CFA and Ag85b was higher in mice vaccinated with 'M. habana' than in animals immunized with BCG.
  
  
  CONCLUSIONS
  these results encourage new research on 'M. habana' as vaccinal candidate against tuberculosis.
authors: Centro Colaborador OPS/OMS, Instituto de Medicina Tropical "Pedro Kourí", La Habana, Cuba. iliana@ipk.sld.cu
authors: Iliana
authors: I
authors: Valdés Hernández
authors: C Ernesto
authors: CE
authors: Montoro Cardoso
authors: Rogelio
authors: R
authors: Hernández-Pando
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009170:Nontuberculous Mycobacteria / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D032581:Tuberculosis Vaccines; C072681:Mycobacterium tuberculosis antigens; D007371:Interferon-gamma