title: Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs.
abstract: Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 °C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.
authors: Department of Orthopaedics, University Medical Center, Utrecht, The Netherlands.
authors: Wouter
authors: W
authors: Schuurman
authors: Peter A
authors: PA
authors: Levett
authors: Michiel W
authors: MW
authors: Pot
authors: Paul René
authors: PR
authors: van Weeren
authors: Wouter J A
authors: WJ
authors: Dhert
authors: Dietmar W
authors: DW
authors: Hutmacher
authors: Ferry P W
authors: FP
authors: Melchels
authors: Travis J
authors: TJ
authors: Klein
authors: Jos
authors: J
authors: Malda
mesh_terms: D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D062028:Bioprinting; D002356:Cartilage / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D019245:Compressive Strength / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism; D006736:Horses; D006820:Hyaluronic Acid / Q000494:pharmacology; D020100:Hydrogels / Q000494:pharmacology*; D007150:Immunohistochemistry; D008422:Materials Testing; D055595:Mechanical Phenomena / Q000187:drug effects; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D000178:Acrylamides; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D006025:Glycosaminoglycans; D020100:Hydrogels; D005780:Gelatin; D006820:Hyaluronic Acid; D004247:DNA; C045985:methacrylamide