title: The effect of substrate and operational parameters on the abundance of sulphate-reducing bacteria in industrial anaerobic biogas digesters.
abstract: This study evaluated the effects of operational parameters and type of substrate on the abundance of sulphate-reducing bacteria in 25 industrial biogas digesters using qPCR targeting the functional dissimilatory sulphite reductase gene. The aim was to find clues for operational strategies minimizing the production of H2S. The results showed that the operation, considering strategies evaluated, only had scarce effect on the abundance, varying between 10(5) and 10(7) gene copies per ml. However, high ammonia levels and increasing concentration of sulphate resulted in significantly lower and higher levels of sulphate-reducing bacteria, respectively.
authors: Tekniska verken i Linköping AB (publ.), Department of Biogas R & D, PO Box 1500, SE-581 15 Linköping, Sweden. jan.moestedt@tekniskaverken.se
authors: Jan
authors: J
authors: Moestedt
authors: Sören
authors: S
authors: Nilsson Påledal
authors: Anna
authors: A
authors: Schnürer
mesh_terms: D000641:Ammonia / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors; D017931:DNA Primers / Q000235:genetics; D006862:Hydrogen Sulfide / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013431:Sulfates / Q000378:metabolism*; D050902:Sulfite Dehydrogenase / Q000235:genetics; D016999:Sulfur-Reducing Bacteria / Q000201:enzymology* / Q000235:genetics; D013548:Sweden
chemical_list: D056804:Biofuels; D017931:DNA Primers; D013431:Sulfates; D000641:Ammonia; D050902:Sulfite Dehydrogenase; D006862:Hydrogen Sulfide