title: The immunomodulatory effect of bone marrow stromal cells (BMSCs) on interleukin (IL)-23/IL-17-mediated ischemic stroke in mice.
abstract: Ischemic cerebral infarction is a leading cause of death and disability, but the key inflammatory cytokines were not fully understood and no successful therapy has been established. We have used the methods of reverse transcriptase-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), western blot, and immunohistochemical staining to detect the dynamic changes of IL-23/IL-17 axis in brain infarction and BMSC treatment on the sixth day. BMSC transplantation could reduce the infarct size (P<0.05) and improve functional deficits (P<0.001) in brain infarction. The level of IL-23/IL-17 axis expression is higher (P<0.05) in pMCAO-operated group than that in sham operated group. BMSC treatment could reduce IL-23 expression (P<0.05) and attenuate IL-17 expression (P<0.01) both in serum and around infarct lesion. So we have drawn the conclusion that IL-23/IL-17 axis induces inflammation in the pathophysiological process of cerebral infarction. BMSC treatment plays therapeutic role by immunomodulating the expression of IL-23/IL-17 axis. These findings may help to understand the cytokines in cerebral infarction and open up a possible new way of immunological treatment.
authors: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 YouZheng Street, Harbin, 150001, HeiLongJiang, PR China.
authors: Shuainan
authors: S
authors: Ma
authors: Di
authors: D
authors: Zhong
authors: Hongping
authors: H
authors: Chen
authors: Yi
authors: Y
authors: Zheng
authors: Yanyan
authors: Y
authors: Sun
authors: Jian
authors: J
authors: Luo
authors: Hulun
authors: H
authors: Li
authors: Guozhong
authors: G
authors: Li
authors: Yanhong
authors: Y
authors: Yin
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002545:Brain Ischemia / Q000276:immunology / Q000378:metabolism* / Q000601:surgery; D056747:Immunomodulation / Q000502:physiology*; D020381:Interleukin-17 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D053759:Interleukin-23 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D020521:Stroke / Q000276:immunology / Q000378:metabolism* / Q000601:surgery
chemical_list: D020381:Interleukin-17; D053759:Interleukin-23