title: Characterization and quantification of racemic and meso-ethylenediamine-N,N'-bis(2-hydroxy-5-sulfophenylacetic) acid/iron (III) by ion-pair ultra-high performance liquid chromatography coupled with diode array detector and electrospray tandem mass spectrometry.
abstract: EDDHSA/Fe is a promising substitute of EDDHA/Fe to fight iron chlorosis. o,o-EDDHSA structure contains two chiral carbons giving the racemic and meso couples of stereoisomers. Ion-pair HPLC and UHPLC-UV/Vis-ESI-MS/MS methods were developed for the determination of racemic and meso-o,o-EDDHSA/Fe in commercial samples of chelates. The lack of a commercial EDDHSA standard was overcome by sulfonation of a commercial available o,o-EDDHA standard and subsequent quantification by (1)H-NMR. Assignment of configurations was carried out starting from racemic and meso-o,o-EDDHA/Fe by direct sulfonation to give the corresponding o,o-EDDHSA/Fe isomers. The performances of these methods were assessed in terms of intra and inter-day precision, linearity and selectivity. The high selectivity and lower detection limit (nanomolar) of the UHPLC-ESI-MS/MS method could allow to deepen the knowledge relative to meso and rac-o,o-EDDHSA/Fe interactions with plants, its fate in different soil conditions, its mobility and other environmental aspects.
authors: Valagro SpA, Via Cagliari 1, 66041 Atessa, Italy. a.biasone@valagro.com
authors: Alessandro
authors: A
authors: Biasone
authors: Giusto
authors: G
authors: Cianci
authors: Donata
authors: D
authors: Di Tommaso
authors: Alberto
authors: A
authors: Piaggesi
authors: Emilio
authors: E
authors: Tagliavini
authors: Paola
authors: P
authors: Galletti
authors: Fabio
authors: F
authors: Moretti
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000144:Acids, Acyclic / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006851:Hydrochloric Acid / Q000737:chemistry; D007477:Ions / Q000032:analysis / Q000737:chemistry; D007501:Iron / Q000032:analysis* / Q000737:chemistry*; D057230:Limit of Detection; D010648:Phenylacetates / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000097:Acetonitriles; D000144:Acids, Acyclic; D007477:Ions; C501683:N,N'-ethylenediamine-di-(2-hidroxy-5-sulfophenylacetic) acid; D010648:Phenylacetates; D007501:Iron; D006851:Hydrochloric Acid; C032159:acetonitrile