title: [Association of tumor necrosis factor-alpha gene polymorphisms with Henoch-Schonlein purpura nephritis in children].
abstract: OBJECTIVE
  To study the relationship of tumor necrosis factor-alpha (TNF-α)-308G/A gene polymorphisms with Henoch-Schonlein purpura nephritis (HSPN) in children.
  
  
  METHODS
  Using the direct DNA sequencing method, polymorphisms in the TNF-α promoter region (-308) were genotyped in 110 Han children with Henoch-Schonlein purpura (HSP group), including 52 children with nephritis and 58 children without nephritis. Plasma TNF-α levels were measured using ELISA. Ninety ethnically matched healthy children were used as the control group.
  
  
  RESULTS
  There were no significant differences in the polymorphisms of TNF-α (-308G/A) between the HSP and control groups (P>0.05). The GA genotype (29% vs 10%) and A allele frequency (18% vs 7%) in HSP children with nephritis (HSPN) were more common than in those without nephritis (P<0.05). Plasma TNF-α levels in HSPN children with GA+AA genotype (7.1±2.3 pg/mL) were significantly higher than those with GG genotype (5.7±1.5 pg/mL) (P<0.05).
  
  
  CONCLUSIONS
  TNF-α-308GA genotype and A allele may contribute to the increased risk for the development of nephritis in children with HSP.
authors: Department of Pediatrics, North Sichuan Medical College, Nanchong, Sichuan, China. wjjsl2003@yahoo.com.cn
authors: Jian-Jun
authors: JJ
authors: Wang
authors: Yan-Ping
authors: YP
authors: Shi
authors: Yue
authors: Y
authors: Huang
authors: Chun
authors: C
authors: Wu
authors: Xu-Chang
authors: XC
authors: Li
mesh_terms: D000293:Adolescent; D002648:Child; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D011695:IgA Vasculitis / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha