title: Production of hydrogen and volatile fatty acid by Enterobacter sp. T4384 using organic waste materials.
abstract: In a study of hydrogen-producing bacteria, strain T4384 was isolated from rice field samples in the Republic of Korea. The isolate was identified as Enterobacter sp. T4384 by phylogenetic analysis of 16S rRNA and rpoB gene sequences. Enterobacter sp. T4384 grew at a temperature range of 10-45 degrees C and at an initial pH range of 4.5-9.5. Strain T4384 produced hydrogen at 0-6% NaCl by using glucose, fructose, and mannose. In serum bottle cultures using a complete medium, Enterobacter sp. T4384 produced 1,098 ml/l H2, 4.0 g/l ethanol, and 1.0 g/l acetic acid. In a pH-regulated jar fermenter culture with the biogas removed, 2,202 ml/l H2, 6.2 g/l ethanol, and 1.0 g/l acetic acid were produced, and the lag-phase time was 4.8 h. Strain T4384 metabolized the hydrolysate of organic waste for the production of hydrogen and volatile fatty acid. The strain T4384 produced 947 ml/l H2, 3.2 g/l ethanol, and 0.2 g/l acetic acid from 6% (w/v) food waste hydrolysate; 738 ml/l H2, 4.2 g/l ethanol, and 0.8 g/l acetic acid from Miscanthus sinensis hydrolysate; and 805 ml/l H2, 5.0 g/l ethanol, and 0.7 g/l acetic acid from Sorghum bicolor hydrolysate.
authors: Energy Materials and Process,, Hanyang University, Seoul, Korea.
authors: Byung-Chun
authors: BC
authors: Kim
authors: Tushar R
authors: TR
authors: Deshpande
authors: Jongsik
authors: J
authors: Chun
authors: Sung Chul
authors: SC
authors: Yi
authors: Hyunook
authors: H
authors: Kim
authors: Youngsoon
authors: Y
authors: Um
authors: Byoung-In
authors: BI
authors: Sang
mesh_terms: D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004754:Enterobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature; D014866:Waste Products
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005232:Fatty Acids, Volatile; D009930:Organic Chemicals; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; D012965:Sodium Chloride; D006859:Hydrogen; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit