title: Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK.
abstract: An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes.
authors: Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin, People's Republic of China. lhhou@tju.edu.cn
authors: Lihua
authors: L
authors: Hou
authors: Xiaoyang
authors: X
authors: Li
authors: Cong
authors: C
authors: Wang
authors: Xiaohong
authors: X
authors: Cao
authors: Haiyong
authors: H
authors: Wang
mesh_terms: D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005800:Genes, Fungal; D010957:Plasmids / Q000235:genetics*; D011003:Ploidies; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C530845:HO protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific