title: Effect of ketamine on the development of Lucilia sericata (Meigen) (Diptera: Calliphoridae) and preliminary pathological observation of larvae.
abstract: The estimation of postmortem interval (PMI) based on the growth patterns of necrophagous arthropods is the main mission of forensic entomology in practice. The larval development rates can be affected by various drugs or toxins, causing deviation in PMI estimate. Ketamine is a widely used anesthetic and recreational drug in Asia, which is rarely focused on in the previous entomotoxicological studies. The present work investigated the effect of ketamine on the development of Lucilia sericata (Meigen) (Diptera: Calliphoridae) by the measurement of body length and weight and the analysis of relationship between the ketamine effect and drug dosage or time interval, meanwhile the difference between ketamine effect on larval body length and weight was also analyzed. Additionally, the preliminary pathological observation of larvae was also employed for evaluating the drug effect in morphology. Significant differences were observed between control and treatment colonies of L. sericata at each life stage, and the effect of ketamine displayed a dosage-and-time-dependent manner, but no differences were noticed between the effects of ketamine on larval body length and weight, which provided a useful indication for larvae sample collection in practice. The pathological observation revealed that ketamine could promote the growth of trophocytes in fat body of L. sericata.
authors: Department of Pathology, The First Clinical Medical College of Fujian Medical University, No. 20 Chazhong Road, Fuzhou 350005, People's Republic of China. eianzou@gmail.com
authors: Yi
authors: Y
authors: Zou
authors: Ming
authors: M
authors: Huang
authors: Ruiting
authors: R
authors: Huang
authors: Xinwu
authors: X
authors: Wu
authors: Zhijie
authors: Z
authors: You
authors: Jieqiong
authors: J
authors: Lin
authors: Xiaoyan
authors: X
authors: Huang
authors: Xiaoting
authors: X
authors: Qiu
authors: Sheng
authors: S
authors: Zhang
mesh_terms: D000778:Anesthetics, Dissociative / Q000494:pharmacology*; D000818:Animals; D004175:Diptera / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D004772:Entomology; D005247:Feeding Behavior; D049429:Forensic Pathology; D008401:Gas Chromatography-Mass Spectrometry; D007649:Ketamine / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D016014:Linear Models; D011180:Postmortem Changes; D011817:Rabbits; D013997:Time Factors
chemical_list: D000778:Anesthetics, Dissociative; D007649:Ketamine