title: Maternal dietary creatine supplementation does not alter the capacity for creatine synthesis in the newborn spiny mouse.
abstract: We have previously reported that maternal creatine supplementation protects the neonate from hypoxic injury. Here, we investigated whether maternal creatine supplementation altered expression of the creatine synthesis enzymes (arginine:glycine amidinotransferase [AGAT], guanidinoaceteate methyltransferase [GAMT]) and the creatine transporter (solute carrier family 6 [neurotransmitter transporter, creatine] member 8: SLC6A8) in the term offspring. Pregnant spiny mice were fed a 5% creatine monohydrate diet from midgestation (day 20) to term (39 days). Placentas and neonatal kidney, liver, heart, and brain collected at 24 hours of age underwent quantitative polymerase chain reaction and Western blot analysis. Maternal creatine had no effect on the expression of AGAT and GAMT in neonatal kidney and liver, but mRNA expression of AGAT in brain tissues was significantly decreased in both male and female neonates born to mothers who were fed the creatine diet. SLC6A8 expression was not affected by maternal dietary creatine loading in any tissues. Maternal dietary creatine supplementation from midgestation in the spiny mouse did not alter the capacity for creatine synthesis or transport.
authors: The Ritchie Centre, Monash Institute of Medical Research, Monash University, Australia. hayley.dickinson@monash.edu
authors: Hayley
authors: H
authors: Dickinson
authors: Zoe J
authors: ZJ
authors: Ireland
authors: Domenic A
authors: DA
authors: Larosa
authors: Bree A
authors: BA
authors: O'Connell
authors: Stacey
authors: S
authors: Ellery
authors: Rod
authors: R
authors: Snow
authors: David W
authors: DW
authors: Walker
mesh_terms: D000579:Amidinotransferases / Q000235:genetics / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism; D003404:Creatinine / Q000008:administration & dosage* / Q000378:metabolism*; D019587:Dietary Supplements; D005260:Female; D005865:Gestational Age; D050937:Guanidinoacetate N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D051189:Murinae; D009206:Myocardium / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D039401:Prenatal Nutritional Physiological Phenomena; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C080679:creatine transporter; D003404:Creatinine; D050937:Guanidinoacetate N-Methyltransferase; D000579:Amidinotransferases; C020681:glycine amidinotransferase
keywords: SLC6A8; arginine:glycine amidinotransferase (AGAT); guanidinoaceteate methyltransferase (GAMT); neonate; placenta