title: Drug delivery and tissue engineering applications of biocompatible pectin-chitin/nano CaCO3 composite scaffolds.
abstract: In this work, we have developed a nanocomposite scaffold using a mixture of pectin, chitin and nano CaCO3 using the technique of lyophilization, with an intended use towards biomedical applications such as tissue engineering and drug delivery. The prepared composite scaffold was characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). In addition, swelling, degradation and biomineralization capability of the composite scaffold was evaluated. The developed composite scaffold showed controlled swelling and degradation in comparison with the control scaffold. Cytocompatibility evaluation of the scaffold was tested on NIH3T3, L929 and human dermal fibroblast (HDF) cells, showed negligible toxicity towards cells. Cell attachment and proliferation studies were also conducted using these cells, which showed that cells attached onto the scaffolds and started to proliferate after 48 h of incubation. Further, drug delivery through the scaffold was examined using a bisphosphonate called Fosamax. These results suggest that the developed composite scaffold possess the essential requisites for their application in the fields of tissue engineering and drug delivery.
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi-682041, India.
authors: P T Sudheesh
authors: PT
authors: Kumar
authors: C
authors: C
authors: Ramya
authors: R
authors: R
authors: Jayakumar
authors: Shanti kumar V
authors: Sk
authors: Nair
authors: Vinoth-Kumar
authors: VK
authors: Lakshmanan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002119:Calcium Carbonate / Q000737:chemistry*; D002460:Cell Line; D002686:Chitin / Q000737:chemistry*; D016503:Drug Delivery Systems; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010368:Pectins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D002686:Chitin; D010368:Pectins; D002119:Calcium Carbonate