title: Intermittent cyclic mechanical tension-induced down-regulation of ectonucleotide pyrophosphatase phosphodiesterase 1 gene expression is mainly dependent on TGF-β1 in end-plate chondrocytes.
abstract: OBJECTIVE
  To investigate the relationship between ectonucleotide pyrophosphatase phosphodiesterase-1(ENPP-1) expression and transforming growth factor beta 1 (TGF-β1) of end-plate chondrocytes after stimulation with intermittent cyclic mechanical tension (ICMT) by using an FX-4000T Flexercell Tension Plus unit.
  
  
  METHODS
  Rat end-plate chondrocytes were cultured and ICMT (strain at 0.5 Hz sinusoidal curve at 10% elongation) applied for 7 days for 4 h/day and cultured for a further 2 days. End-plate chondrocytes were also exposed to 10 ng/mL of TGF-β1. Then, using small interfering RNA technology, small interfering TGF-β1 (siTGF-β1) was transfected. Expression of ENPP-1 and TGF-β1 was measured by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and western blotting.
  
  
  RESULTS
  Expression of both ENPP-1 and TGF-β1 was up-regulated after ICMT. Both RT-PCR and western blot showed that ENPP-1 expression decreases with siRNA TGF-β1 after 3% elongation 40 min, and cultured for an additional 2 days.
  
  
  CONCLUSIONS
  It was found that down-regulation of ENPP-1 gene expression induced by ICMT is likely dependent on TGF-β1 in end-plate chondrocytes.
authors: Department of Spine Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, China. xuhg@medmail.com.cn
authors: Hong-guang
authors: HG
authors: Xu
authors: Zi-rui
authors: ZR
authors: Li
authors: Hong
authors: H
authors: Wang
authors: Ping
authors: P
authors: Liu
authors: Sheng-nan
authors: SN
authors: Xiang
authors: Chuang-dong
authors: CD
authors: Wang
authors: Xiao-ling
authors: XL
authors: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D006132:Growth Plate / Q000187:drug effects / Q000378:metabolism*; D008159:Lumbar Vertebrae / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D034741:RNA, Small Interfering; D053773:Transforming Growth Factor beta1; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases