title: Nuclear magnetic resonance methods for metabolic fluxomics.
abstract: Fluxomics, through its core methodology of metabolic flux analysis (MFA), enables quantification of carbon traffic through cellular biochemical pathways. Isotope labeling experiments aid MFA by providing information on intracellular fluxes, especially through parallel and cyclic pathways. Nuclear magnetic resonance (NMR) and mass spectrometry (MS) are two complementary methods to measure abundances of isotopomers generated in these experiments. 2-D [(13)C, (1)H] heteronuclear correlation NMR spectra can detect (13)C isotopes coupled to protons and thus noninvasively separate molecules and atoms with a specific isotopic content from a mixture of molecular species. Furthermore, the fine structures of the peaks in these spectra can reveal scalar couplings between chemically bonded carbon atoms in the sample, from which isotopomer abundances can be quantified. This chapter introduces methods for NMR sample preparation and spectral acquisition of 2-D [(13)C, (1)H] correlation maps, followed by a detailed presentation of methods to process the spectra and quantify isotopomer abundances. We explain the use of the software NMRViewJ for spectral visualization and processing, as well as MATLAB scripts developed by us for peak extraction, deconvolution of overlapping peaklets, and isotopomer abundance quantification. Finally, we discuss the applications of NMR-derived isotopomer data toward quantitatively understanding metabolic pathways.
authors: Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD, USA.
authors: Shilpa
authors: S
authors: Nargund
authors: Max E
authors: ME
authors: Joffe
authors: Dennis
authors: D
authors: Tran
authors: Vitali
authors: V
authors: Tugarinov
authors: Ganesh
authors: G
authors: Sriram
mesh_terms: D000465:Algorithms; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism; D007553:Isotope Labeling; D060847:Metabolic Engineering; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012984:Software
chemical_list: D002247:Carbon Isotopes