title: Viral load of human bocavirus-1 in stools from children with viral diarrhoea in Paraguay.
abstract: Since their discovery, four species of human bocavirus (HBoV) have been described in patients with respiratory and gastrointestinal diseases. However, a clear causal association between HBoV-1 and gastroenteritis has not been demonstrated. In this study, we describe the detection and quantification of HBoV-1 in stools from children with acute non-bacterial gastroenteritis using quantitative polymerase chain reaction. HBoV-1 genome was detected in 10.6% of stools with frequent association with rotavirus and norovirus. The median of HBoV-1 viral load was 1.88 × 104 genome/ml, lower than previously shown in secretions of patients with respiratory infections, without any obvious association between high viral load and presence of HBoV as single agent. Thus, although HBoV-1 was frequently detected in these patients, there is no clear causal association of this agent with diarrhoea. Indeed, HBoV-1 DNA in stools of patients with gastroenteritis without respiratory symptoms may be a remnant of previous infections or associated with prolonged shedding of virus in the respiratory or digestive tracts.
authors: Departamento de Biologia Celular e Molecular, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors: J L
authors: JL
authors: Proenca-Modena
authors: M
authors: M
authors: Martinez
authors: A A
authors: AA
authors: Amarilla
authors: E E
authors: EE
authors: Espínola
authors: M E
authors: ME
authors: Galeano
authors: N
authors: N
authors: Fariña
authors: G
authors: G
authors: Russomando
authors: V H
authors: VH
authors: Aquino
authors: G I
authors: GI
authors: Parra
authors: E
authors: E
authors: Arruda
mesh_terms: D002675:Child, Preschool; D060085:Coinfection / Q000821:virology; D003430:Cross-Sectional Studies; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000821:virology; D056661:Human bocavirus / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010239:Paraguay / Q000453:epidemiology; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D019562:Viral Load; D014777:Virus Diseases / Q000821:virology*