title: Preparation of an immunoaffinity column with amino-silica gel microparticles and its application in sample cleanup for aflatoxin detection in agri-products.
abstract: This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G₁, B₁, G₂ and B₂ were 0.03, 0.07, 0.05 and 0.09 μg·kg⁻¹, and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg⁻¹, respectively. The recoveries of aflatoxins in samples were 90.1%-104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg⁻¹.
authors: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors: Fei
authors: F
authors: Ma
authors: Ran
authors: R
authors: Chen
authors: Peiwu
authors: P
authors: Li
authors: Qi
authors: Q
authors: Zhang
authors: Wen
authors: W
authors: Zhang
authors: Xiaofeng
authors: X
authors: Hu
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000383:Agriculture; D000588:Amines / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D057230:Limit of Detection; D008863:Microspheres; D009821:Oils / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D058428:Silica Gel / Q000737:chemistry*; D012996:Solutions; D012997:Solvents; D019265:Spectroscopy, Near-Infrared; D013662:Tea / Q000737:chemistry
chemical_list: D000348:Aflatoxins; D000588:Amines; D000906:Antibodies; D009821:Oils; D012996:Solutions; D012997:Solvents; D013662:Tea; D058428:Silica Gel