title: Electrospun silk fibroin nanofibers in different diameters support neurite outgrowth and promote astrocyte migration.
abstract: Nerve tissue engineering has been one of the promising strategies for regenerative treatment in patients suffering from neural tissue loss, but considerable challenges remain before it is able to progress toward clinical application. It has been demonstrated that transplantation of cells in combination with physically or chemically modified biomaterials provides better environments for neurite outgrowth and further promotes axonal regeneration in animal models of spinal cord injury. In this study, neurons and astrocytes were incorporated into 400-nm, 800-nm, and 1200-nm electrospun Bombyx mori silk fibroin (SF) materials to investigate the effects of scaffold-diameter in regulating and directing cell behaviors. β-III-tubulin immunofluorescence analyses reveal that SF nanofibers with smaller diameters are more favorable to the development and maturation of subventricular zone-derived neurons than 1200-nm SF scaffolds. In addition, astrocytes exhibited well-arranged glial fibrillary acidic protein (GFAP) expression on SF scaffolds, and a significant increase in cell-spreading area was observed on 400-nm but not 1200-nm SF scaffolds. Moreover, a significantly enhanced migration efficiency of astrocytes grown on SF scaffolds was verified, which highlights the guiding roles of SF nanofibers to the migratory cells. Overall, our results may provide valuable information to develop effective tissue remodeling substrates and to optimize existing biomaterials for neural tissue engineering applications.
authors: Department of Cell Biology, Jiangsu Key Laboratory of Stem Cell Research, Medical College of Soochow University, Suzhou Industrial Park, Suzhou 215123, China.
authors: Jing
authors: J
authors: Qu
authors: Dan
authors: D
authors: Wang
authors: Huihui
authors: H
authors: Wang
authors: Yunhai
authors: Y
authors: Dong
authors: Feng
authors: F
authors: Zhang
authors: Baoqi
authors: B
authors: Zuo
authors: Huanxiang
authors: H
authors: Zhang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology* / Q000648:ultrastructure; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D005349:Fibroins / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology; D016501:Neurites / Q000502:physiology* / Q000648:ultrastructure; D009457:Neuroglia / Q000166:cytology / Q000502:physiology; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005349:Fibroins
keywords: astrocyte; diameter; growth behavior; neuron; silk fibroin