title: Two natural recombinant highly pathogenic porcine reproductive and respiratory syndrome viruses with different pathogenicities.
abstract: Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is a variant of type 2 PRRSV with high virulence. Genetic and pathogenic characteristics of HP-PRRSV vary rapidly during the evolution. In this study, we determined the complete genome of a HP-PRRSV isolate designated 10FUJ-2, which shared 98.34 % nucleotide identity with HP-PRRSV reference strain JXA1. Genomic analyses by phylogenetic tree and recombination detection program confirmed 10FUJ-2 to be a recombinant with 09JS and JXA1 as potential parental viruses. Furthermore, we identified that 10FUJ-2 has high virulence as similar as the parental viruses by animal challenge study. In addition, we found that SY0909 was also a recombination virus probably from JXA1 and NT0801, which has been reported to be low pathogenic. Recombination analysis also revealed that Glycoproteins GP2 to GP5 of HP-PRRSV might contain major virulence determinants. Identification of two natural recombinants with different virulence supports the notion that recombination is a driving force affecting HP-PRRSV pathogenicity and a common mechanism contributing to HP-PRRSV evolution.
authors: Veterinary Diagnostic Lab, China Animal Disease Control Center, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
authors: Nanhua
authors: N
authors: Chen
authors: Xiuling
authors: X
authors: Yu
authors: Lilin
authors: L
authors: Wang
authors: Jiajun
authors: J
authors: Wu
authors: Zhi
authors: Z
authors: Zhou
authors: Jianqiang
authors: J
authors: Ni
authors: Xiangdong
authors: X
authors: Li
authors: Xinyan
authors: X
authors: Zhai
authors: Kegong
authors: K
authors: Tian
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000473:pathology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D014764:Viral Proteins / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D006023:Glycoproteins; D012367:RNA, Viral; D014764:Viral Proteins; D037521:Virulence Factors