title: Real space flight travel is associated with ultrastructural changes, cytoskeletal disruption and premature senescence of HUVEC.
abstract: Microgravity, hypergravity, vibration, ionizing radiation and temperature fluctuations are major factors of outer space flight affecting human organs and tissues. There are several reports on the effect of space flight on different human cell types of mesenchymal origin while information regarding changes to vascular endothelial cells is scarce. Ultrastructural and cytophysiological features of macrovascular endothelial cells in outer space flight and their persistence during subsequent culturing were demonstrated in the present investigation. At the end of the space flight, endothelial cells displayed profound changes indicating cytoskeletal lesions and increased cell membrane permeability. Readapted cells of subsequent passages exhibited persisting cytoskeletal changes, decreased metabolism and cell growth indicating cellular senescence.
authors: Institute of Medical Molecular Biotechnology (IMMB), Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia.
authors: M Y
authors: MY
authors: Kapitonova
authors: S
authors: S
authors: Muid
authors: G R A
authors: GR
authors: Froemming
authors: W N W
authors: WN
authors: Yusoff
authors: S
authors: S
authors: Othman
authors: A M
authors: AM
authors: Ali
authors: H M
authors: HM
authors: Nawawi
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D003599:Cytoskeleton / Q000648:ultrastructure*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000502:physiology; D008855:Microscopy, Electron, Scanning / Q000379:methods; D013026:Space Flight; D013997:Time Factors; D014893:Weightlessness / Q000009:adverse effects*