title: Intraneural OX7-saporin for neuroma-in-continuity in a rat model.
abstract: We employed 54 rats to devise a model of neuroma-in-continuity and explore the effect of the immunotoxin OX7-saporin on the neuroma. The left common peroneal, tibial or sciatic nerves were crushed by one 10-s application of a micro-artery forceps. At 3 and 6 weeks, the nerve was cut distal to the site of nerve crush, and retrograde fluorescent labeling was done. Pressure microinjection of 2 μl of natural saline or 2 μl of the immunotoxin conjugate OX7-saporin was done at the nerve stump 2 days later. Sacrifice was done after 3 weeks. In all control and saline-injection nerve specimens, gross observation and histology showed a neuroma-in-continuity. In 19 of the 24 OX7-saporin nerve specimens, gross observation showed a narrowed area at the site of nerve crush. Histology showed inhibition of neuroma-in-continuity formation. Fluorescent microscopy showed ablation of the labeled neurons in the dorsal root ganglia corresponding to the OX7-saporin subgroups.
authors: First Department of Orthopaedics, Athens University Medical School, 41 Ventouri Street, Holargos, Athens, Greece. afm@otenet.gr
authors: Andreas F
authors: AF
authors: Mavrogenis
authors: Kitty
authors: K
authors: Pavlakis
authors: Anna
authors: A
authors: Stamatoukou
authors: Panayiotis J
authors: PJ
authors: Papagelopoulos
authors: Stamatis
authors: S
authors: Theoharis
authors: Zijie
authors: Z
authors: Zetahang
authors: Panayotis N
authors: PN
authors: Soucacos
authors: Aristides B
authors: AB
authors: Zoubos
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000187:drug effects; D018796:Immunoconjugates / Q000008:administration & dosage / Q000627:therapeutic use*; D000922:Immunotoxins / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D008845:Microinjections / Q000379:methods; D008856:Microscopy, Fluorescence; D009409:Nerve Crush; D059348:Peripheral Nerve Injuries / Q000188:drug therapy* / Q000473:pathology; D010543:Peroneal Nerve / Q000293:injuries / Q000473:pathology; D051381:Rats; D054789:Ribosome Inactivating Proteins, Type 1 / Q000008:administration & dosage / Q000627:therapeutic use*; D000076985:Saporins; D012584:Sciatic Nerve / Q000293:injuries / Q000473:pathology; D013979:Tibial Nerve / Q000293:injuries / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D000922:Immunotoxins; C085346:OX7-saporin; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins