title: CGH protocols: chronic lymphocytic leukemia.
abstract: Array-based comparative genomic hybridization (aCGH) is a powerful assay to identify copy number abnormalities underlying the pathogenesis of cancer. aCGH has become the gold standard for whole genome copy number analysis in medium and large cohorts in clinical and research laboratories. Identifying the best workflow is critical to achieving the optimal performance for this assay. Here we describe the aCGH protocol used by our group in the study of B-chronic lymphocytic leukemia (CLL). We also describe some initial applications of aCGH in association with clinical outcome for CLL.
authors: Mayo Clinic Arizona, Scottsdale, AZ, USA. braggio.esteban@mayo.edu
authors: Esteban
authors: E
authors: Braggio
authors: Rafael
authors: R
authors: Fonseca
authors: Neil E
authors: NE
authors: Kay
mesh_terms: D000818:Animals; D055028:Comparative Genomic Hybridization / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000235:genetics*; D053938:DNA Fragmentation; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004247:DNA