title: 5-Aza-2'-deoxycytidine may  influence the proliferation and apoptosis of cervical cancer cells via  demethylation in a dose- and time-dependent manner.
abstract: The  methylation of tumor suppressor genes has been shown to be involved in many  human cancers. 5-Aza-2'-deoxycytidine  (5-Aza-CdR) can reactivate the expression of methylated tumor suppressor genes.  In our study, 2 human cervical cancer cell lines, HeLa and SiHa, were treated  with different concentrations (20, 10, 5, and 2.5 μM) of 5-Aza-CdR for 24, 48, and 72  h. After incubation, cells were analyzed by  3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow  cytometry. The expression of RASSF1A and APAF-1 was detected by  RT-PCR. 5-Aza-CdR inhibited the growth of HeLa and SiHa cells at different  concentrations. The strongest inhibition and apoptosis rates were obtained  after incubation for 72 h (5.63 ± 1.38 and 8.24 ± 2.40%, respectively). No  significant difference in the expression of RASSF1A was found upon drug  treatment, while APAF-1 expression increased in HeLa cells after  treatment (0.790 ± 0.056%). Our results suggest that the tumor-suppressive  effect of 5-Aza-CdR may result from the reactivation of silenced APAF-1 through  demethylation.
authors: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, PR China.
authors: T T
authors: TT
authors: Yao
authors: S M
authors: SM
authors: Mo
authors: L Y
authors: LY
authors: Liu
authors: H W
authors: HW
authors: Lu
authors: M L
authors: ML
authors: Huang
authors: Z Q
authors: ZQ
authors: Lin
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D048708:Cell Growth Processes / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000187:drug effects*; D000077209:Decitabine; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D025521:Tumor Suppressor Proteins / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology
chemical_list: C506592:APAF1 protein, human; D053477:Apoptotic Protease-Activating Factor 1; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins; D000077209:Decitabine; D001374:Azacitidine