title: An ion-pair principle for enantioseparations of basic analytes by nonaqueous capillary electrophoresis using the di-n-butyl L-tartrate-boric acid complex as chiral selector.
abstract: A chiral recognition mechanism of ion-pair principle has been proposed in this study. It rationalized the enantioseparations of some basic analytes using the complex of di-n-butyl l-tartrate and boric acid as the chiral selector in methanolic background electrolytes (BGEs) by nonaqueous capillary electrophoresis (NACE). An approach of mass spectrometer (MS) directly confirmed that triethylamine promoted the formation of negatively charged di-n-butyl l-tartrate-boric acid complex chiral counter ion with a complex ratio of 2:1. And the negatively charged counter ion was the real chiral selector in the ion-pair principle enantioseparations. It was assumed that triethylamine should play its role by adjusting the apparent acidity (pH*) of the running buffer to a higher value. Consequently, the effects of various basic electrolytes including inorganic and organic ones on the enantioseparations in NACE were investigated. The results showed that most of the basic electrolytes tested were favorable for the enantioseparations of basic analytes using di-n-butyl l-tartrate-boric acid complex as the chiral ion-pair selector.
authors: National Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors: Li-Juan
authors: LJ
authors: Wang
authors: Xiu-Feng
authors: XF
authors: Liu
authors: Qie-Nan
authors: QN
authors: Lu
authors: Geng-Liang
authors: GL
authors: Yang
authors: Xing-Guo
authors: XG
authors: Chen
mesh_terms: D000588:Amines / Q000032:analysis / Q000302:isolation & purification*; D064753:Ammonium Hydroxide; D001888:Boric Acids / Q000737:chemistry*; D002021:Buffers; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry; D008956:Models, Chemical; D012972:Sodium Hydroxide / Q000737:chemistry; D013237:Stereoisomerism; D013644:Tartrates / Q000737:chemistry*
chemical_list: D000588:Amines; D001888:Boric Acids; D002021:Buffers; D004573:Electrolytes; D006878:Hydroxides; D013644:Tartrates; D064753:Ammonium Hydroxide; D012972:Sodium Hydroxide; C032688:boric acid