title: Association of microRNA-181c expression with the progression and prognosis of human gastric carcinoma.
abstract: OBJECTIVE
  The relationship of miR-181c, a potential tumor regulatory factor, with gastric cancer is not well studied. We have investigated this relationship in our study.
  
  
  METHODS
  Paraffin-embedded tissue specimens from 103 gastric cancer patients were subjected to total RNA extraction. Reverse transcription real-time fluorescence quantitative PCR was used to detect miR-181c expression. Its relative expression was correlated with the patients’ clinicopathological features. Also, the 5-year survival rate and median survival time were correlated with the miR-181c expression level.
  
  
  RESULTS
  miR-181c expression was significantly and directly correlated with the degree of tumor differentiation, invasive depth and clinical stage. Moreover, lymph node metastasis was significantly related with higher miR-181c expression. However, miR-181c expression was not significantly correlated with gender, age, tumor location and distant metastasis. The 5-year overall survival rate and median survival time were significantly greater in patients with low expression of miR-181c.
  
  
  CONCLUSIONS
  miR-181c expression level was significantly related to several clinicopathological features of gastric cancer; therefore, miR-181c probably plays a role in its development and progression. This relationship needs to be studied in detail, so that the potential use of miR-181c as a marker for gastric cancer and therapeutic target can be explored.
authors: Meihua
authors: M
authors: Cui
authors: Lin
authors: L
authors: Yue
authors: Yi
authors: Y
authors: Fu
authors: Weidong
authors: W
authors: Yu
authors: Xiaolin
authors: X
authors: Hou
authors: Xiuru
authors: X
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D008875:Middle Aged; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*
chemical_list: C540193:MIrn181 microRNA, human; D035683:MicroRNAs