title: Effect of contact lens wear on the release of tear mediators in keratoconus.
abstract: OBJECTIVE
  The release of different cytokines and mediators in tears of patients with keratoconus (KC) wearing contact lenses (CLs) may contribute to the pathology of KC.
  
  
  METHODS
  Cohort study was established in patients with KC wearing rigid gas permeable (RGP) CL (group I), patients with ametropia wearing silicone hydrogel (Si-Hi) CL (group II) and ametropic patients wearing RGP CL (group III).
  
  
  RESULTS
  Our findings indicate that before CL wear, the release of epidermal growth factor (EGF) and tissue-type plasminogen activator (t-PA) was attenuated, whereas matrix metalloproteinase (MMP)-9, interleukin (IL)-6, chemokine (C-C motif) ligand 5 (CCL5), IL-13, and plasminogen activator inhibitor (PAI)-1 were enhanced in KC compared with ametropes. An increasing linear trend over time was found for MMP-9, EGF, and CXCL8 in KC and MMP-9, MMP-13, IL-6, and CXCL8 in group III. Significant differences were observed in the linear trend over time between groups I and III for MMP-13 and tissue inhibitor of metalloproteinases (TIMP)-1; between groups I and II for MMP-9 and CXCL8; and between groups III and II for MMP-9, CXCL8, and MMP-13. In KC, the release of MMP-9 at week 6 and nerve growth factor (NGF) at 10 min was higher, but NGF at week 2 was lower than that in group II. The release of MMP-13 and NGF at week 2 and 6 were lower in the KC group as compared with group III, and similarly, with IL-6 and CXCL8 at week 2 and PAI at all time points.
  
  
  CONCLUSIONS
  Contact lens wear can influence the levels and dynamics of various mediators in the tears of patients with KC that might have an impact on the progression of the disease.
authors: Departments of Ophthalmology, Faculty of Medicine, School of Public Health, Medical and Health Sciences Centre, University of Debrecen, H-4012 Debrecen, Hungary.
authors: Mariann
authors: M
authors: Fodor
authors: Bence L
authors: BL
authors: Kolozsvári
authors: Goran
authors: G
authors: Petrovski
authors: Beáta A
authors: BA
authors: Kettesy
authors: Péter
authors: P
authors: Gogolák
authors: Éva
authors: É
authors: Rajnavölgyi
authors: Bernadett
authors: B
authors: Ujhelyi
authors: László
authors: L
authors: Módis
authors: Beáta É
authors: BÉ
authors: Petrovski
authors: Georgina Z
authors: GZ
authors: Szima
authors: András
authors: A
authors: Berta
authors: Andrea
authors: A
authors: Facskó
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015415:Biomarkers / Q000378:metabolism; D015331:Cohort Studies; D003263:Contact Lenses, Hydrophilic; D016207:Cytokines / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D007640:Keratoconus / Q000378:metabolism*; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D008875:Middle Aged; D013666:Tears / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; D005136:Eye Proteins; C042434:tear proteins; D020782:Matrix Metalloproteinases