title: Vitamin D and 25-hydroxyvitamin D determination in meats by LC-IT-MS.
abstract: This paper reports a method for the rapid, sensitive and simultaneous analysis of vitamin D (Vit D) and 25-hydroxyvitamin D (25OH-Vit D) in meats. Samples were saponified and underwent solid phase extraction with analysis by normal phase liquid chromatography (LC) with ion trap mass spectroscopy (IT-MS), using positive polarity atmospheric pressure chemical ionisation (APCI). Limits of detection (LOD) and quantification (LOQ) for Vit D and 25OH-Vit D were 0.03 and 0.05 μg/100g respectively. Deuterium labelled Vit D and 25OH-Vit D internal standards were added as surrogates prior to saponification, correcting for extraction inefficiencies and potential MS matrix enhancement or suppression effects. Recoveries using internal/surrogate standard correction ranged from 80% to 100% for all vitamers. Measurement uncertainty ranged from 6% to 15% for all vitamers in this method. This process required only 7.5 g of sample per extraction and a batch of 28 extractions could be completed in six hours.
authors: National Measurement Institute (NMI), 1/153 Bertie Street, Port Melbourne, Victoria 3207, Australia. norbert.strobel@measurement.gov.au
authors: Norbert
authors: N
authors: Strobel
authors: Saman
authors: S
authors: Buddhadasa
authors: Paul
authors: P
authors: Adorno
authors: Katherine
authors: K
authors: Stockham
authors: Heather
authors: H
authors: Greenfield
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D052616:Solid Phase Extraction; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D014807:Vitamin D; C104450:25-hydroxyvitamin D