title: Global identification of miRNAs and targets in Populus euphratica under salt stress.
abstract: Populus euphratica, a typical hydro-halophyte, is ideal for studying salt stress responses in woody plants. MicroRNAs (miRNAs) are endogenous non-coding small RNAs that fulfilled an important post-transcriptional regulatory function. MiRNA may regulate tolerance to salt stress but this has not been widely studied in P. euphratica. In this investigation, the small RNAome, degradome and transcriptome were studied in salt stress treated P. euphratica by deep sequencing. Two hundred and eleven conserved miRNAs between Populus trichocarpa and P. euphratica have been found. In addition, 162 new miRNAs, belonging to 93 families, were identified in P. euphratica. Degradome sequencing experimentally verified 112 targets that belonged to 51 identified miRNAs, few of which were known previously in P. euphratica. Transcriptome profiling showed that expression of 15 miRNA-target pairs displayed reverse changing pattern under salt stress. Together, these results indicate that, in P. euphratica under salt stress, a large number of new miRNAs could be discovered, and both known and new miRNA were functionally cleaving to their target mRNA. Expression of miRNA and target were correspondingly induced by salt stress but that it was a complex process in P. euphratica.
authors: College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35, Tsinghua East Road, Beijing, 100083, People's Republic of China.
authors: Bosheng
authors: B
authors: Li
authors: Hui
authors: H
authors: Duan
authors: Jigang
authors: J
authors: Li
authors: Xing Wang
authors: XW
authors: Deng
authors: Weilun
authors: W
authors: Yin
authors: Xinli
authors: X
authors: Xia
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D032107:Populus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D059367:RNA Cleavage; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D012965:Sodium Chloride