title: Astasia and pyrexia related to Borrelia garinii infection in two dogs in Hokkaido, Japan.
abstract: Two dogs that exhibited sudden astasia, anorexia and fever higher than 40°C were suspected of having Lyme disease in July 2011. Clinical symptoms gradually improved with antibiotic treatment in both cases. Polymerase chain reaction and sequence analysis revealed Borrelia garinii DNA fragments in the peripheral blood in the acute disease phase. Serological tests, including enzyme linked immunosorbent assay and Western blot analysis, showed an increased IgG antibody titer against Borrelia pathogens in one of the dogs. These findings suggested that diagnosis of the two dogs was Lyme disease related to B. garinii infection.
authors: Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan. inokuma@obihiro.ac.jp
authors: Hisashi
authors: H
authors: Inokuma
authors: Shigeki
authors: S
authors: Maetani
authors: Jyunji
authors: J
authors: Fujitsuka
authors: Ai
authors: A
authors: Takano
authors: Kozue
authors: K
authors: Sato
authors: Takashi
authors: T
authors: Fukui
authors: Toshiyuki
authors: T
authors: Masuzawa
authors: Hiroki
authors: H
authors: Kawabata
mesh_terms: D000667:Ampicillin / Q000627:therapeutic use; D000818:Animals; D001259:Ataxia / Q000209:etiology / Q000662:veterinary*; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D016000:Cluster Analysis; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005334:Fever / Q000209:etiology / Q000662:veterinary*; D007564:Japan; D008193:Lyme Disease / Q000150:complications / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D016896:Treatment Outcome
chemical_list: D000667:Ampicillin