title: Human complement receptor type 1/CD35 is an Epstein-Barr Virus receptor.
abstract: Epstein-Barr virus (EBV) attachment to primary B cells initiates virus entry. Although CD21 is the only known receptor for EBVgp350/220, a recent report documents EBV-infected B cells from a patient genetically deficient in CD21. On normal resting B cells, CD21 forms two membrane complexes: one with CD19 and another with CD35. Whereas the CD21/CD19 complex is widely retained on immortalized and B cell tumor lines, the related complement-regulatory protein CD35 is lost. To determine the role(s) of CD35 in initial infection, we transduced a CD21-negative pre-B cell and myeloid leukemia line with CD35, CD21, or both. Cells expressing CD35 alone bound gp350/220 and became latently infected when the fusion receptor HLA II was coexpressed. Temporal, biophysical, and structural characteristics of CD35-mediated infection were distinct from CD21. Identification of CD35 as an EBV receptor uncovers a salient role in primary infection, addresses unsettled questions of virus tropism, and underscores the importance of EBVgp350/220 for vaccine development.
authors: Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors: Javier G
authors: JG
authors: Ogembo
authors: Lakshmi
authors: L
authors: Kannan
authors: Ionita
authors: I
authors: Ghiran
authors: Anne
authors: A
authors: Nicholson-Weller
authors: Robert W
authors: RW
authors: Finberg
authors: George C
authors: GC
authors: Tsokos
authors: Joyce D
authors: JD
authors: Fingeroth
mesh_terms: D018941:Antigens, CD19 / Q000378:metabolism; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D009154:Mutation; D054448:Precursor Cells, B-Lymphoid / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D017463:Receptors, Complement 3b / Q000235:genetics / Q000378:metabolism*; D017464:Receptors, Complement 3d / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014162:Transfection; D014763:Viral Matrix Proteins / Q000378:metabolism; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D018941:Antigens, CD19; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D014763:Viral Matrix Proteins