title: Association of methionine synthase and thymidylate synthase genetic polymorphisms with idiopathic recurrent pregnancy loss.
abstract: OBJECTIVE
  To investigate the association between one-carbon metabolism and recurrent pregnancy loss (RPL). One-carbon metabolism is important for maintaining pregnancy, and the enzymes codified by these genes are relevant to this metabolic pathway.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  An urban university-based hospital in South Korea.
  
  
  METHODS
  A cohort of 353 RPL patients (3.09 ± 1.65 pregnancy losses) and 226 control subjects.
  
  
  METHODS
  None.
  
  
  METHODS
  Genotyping was assessed by polymerase chain reaction-restriction fragment length polymorphism assay. We examined polymorphisms in four genes: methionine synthase (MTR); methionine synthase reductase (MTRR); methylenetetrahydrofolate dehydrogenase 1 (MTHFD1); and thymidylate synthase (TS).
  
  
  RESULTS
  The MTR 2756AA polymorphism was associated with RPL. Gene-gene interaction analysis revealed that the frequency of the MTR 2756A-TS 6-bp allele combination was significantly higher in RPL.
  
  
  CONCLUSIONS
  Based on these results, we propose that the MTR 2756AA genotype and MTR 2756A-TS 6-bp allele combination are possible predisposing factors for RPL development in Korean women.
authors: Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea.
authors: Ji Hyang
authors: JH
authors: Kim
authors: Young Joo
authors: YJ
authors: Jeon
authors: Bo Eun
authors: BE
authors: Lee
authors: Hojeong
authors: H
authors: Kang
authors: Ji Eun
authors: JE
authors: Shin
authors: Dong Hee
authors: DH
authors: Choi
authors: Woo Sik
authors: WS
authors: Lee
authors: Nam Keun
authors: NK
authors: Kim
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics*; D000026:Abortion, Habitual / Q000201:enzymology / Q000453:epidemiology* / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D056726:Genetic Association Studies / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011247:Pregnancy; D056910:Republic of Korea / Q000453:epidemiology; D013940:Thymidylate Synthase / Q000235:genetics*; D055815:Young Adult
chemical_list: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D013940:Thymidylate Synthase