title: Differential proteomic analysis in human cells subjected to ribosomal stress.
abstract: The biochemical phenotype of cells affected by ribosomal stress has not yet been studied in detail. Here we report a comparative proteomic analysis of cell lines silenced for the RPS19 gene versus cell lines transfected with scramble shRNA cells performed using the DIGE technology integrated to bioinformatics tools. Importantly, to achieve the broadest possible understanding of the outcome, we carried out two independent DIGE experiments using two different pH ranges, thus, allowing the identification of 106 proteins. Our data revealed the deregulation of proteins involved in cytoskeleton reorganization, PTMs, and translation process. A subset (26.9%) of these proteins is translated from transcripts that include internal ribosome entry site motifs. This supports the hypothesis that during ribosomal stress translation of specific messenger RNAs is altered.
authors: Fondazione SDN-IRCCS, Napoli, Italy. margherita.ruoppolo@unina.it
authors: Marianna
authors: M
authors: Caterino
authors: Claudia
authors: C
authors: Corbo
authors: Esther
authors: E
authors: Imperlini
authors: Marta
authors: M
authors: Armiraglio
authors: Elisa
authors: E
authors: Pavesi
authors: Anna
authors: A
authors: Aspesi
authors: Fabrizio
authors: F
authors: Loreni
authors: Irma
authors: I
authors: Dianzani
authors: Margherita
authors: M
authors: Ruoppolo
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D040901:Proteomics / Q000379:methods*; D034741:RNA, Small Interfering / Q000378:metabolism; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D034741:RNA, Small Interfering; D012269:Ribosomal Proteins; C040343:ribosomal protein S19