title: Anti-neutrophilic inflammatory secondary metabolites from the traditional Chinese medicine, Tiankuizi.
abstract: Through bioassay-guided fractionation, thirteen compounds (1-13) were isolated from the dry root of Semiaquilegia adoxoides, known as Tiankuizi in traditional Chinese medicine (TCM). Among these, four benzoic acid derivatives (1, 2, 4, 5), one 4,6-dimethoxy-5-methyl-2H-pyran-2-one (10) and one 1,2,3-propanetriol (13) were found for the first time in S. adoxoides. This is the first record of compound 10 from a natural source. 4-Hydroxybenzoic acid (1) and 3,4-dihyroxybenzoic acid (2) showed selective inhibition against elastase release and superoxide anion generation, with IC50 values of 3.20 and 6.21 microg/mL, respectively. Compound 1 had 7-fold better activity than the positive control against elastase release induced by human neutrophils. Overall, our studies demonstrated Tiankuizi (S. adoxoides) as a potential TCM and isolates 1 and 2 as promising lead compounds for neutrophilic inflammatory diseases.
authors: School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
authors: Chia-Lin
authors: CL
authors: Lee
authors: Tsong-Long
authors: TL
authors: Hwang
authors: Chieh-Yu
authors: CY
authors: Peng
authors: Chao-Jung
authors: CJ
authors: Chen
authors: Yuan-Shiun
authors: YS
authors: Chang
authors: Yang-Chang
authors: YC
authors: Wu
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology*; D001681:Biological Assay; D002470:Cell Survival; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D020556:Neutrophil Infiltration / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D053491:Proteinase Inhibitory Proteins, Secretory / Q000737:chemistry / Q000494:pharmacology; D031948:Semiaquilegia / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001565:Benzoates; D007202:Indicators and Reagents; D010936:Plant Extracts; D053491:Proteinase Inhibitory Proteins, Secretory; D013481:Superoxides; C578812:tiankuizi