title: Purification and identification of antihypertensive peptides from fermented buckwheat sprouts.
abstract: Buckwheat (Fagopyrum esculentum) is rich in antihypertensive compounds. This study investigated the effect of lactic-fermented buckwheat sprouts (neo-FBS) on level, identification, and potency of blood pressure-lowering (BPL) compounds. A single oral dose of 1.0 mg/kg body weight buckwheat sprouts (BS) in spontaneously hypertensive rats did not show significant BPL activity, whereas neo-FBS significantly decreased blood pressure. HPLC of neo-FBS identified two peaks absent in the profile of BS. The peak exhibiting potent BPL activity was fractionated, and six peptides (DVWY, FDART, FQ, VAE, VVG, and WTFR) and tyrosine were identified by LC-MS/MS and Edman degradation. Single oral dose administration of the peptides revealed significant BPL effect of all the peptides, with the most potent being DVWY, FQ, and VVG. DVWY, VAE, and WTFR are novel. This study demonstrates that lactic fermentation of BS produces new, highly potent antihypertensive peptides and increases active compounds GABA and tyrosine already present in BS.
authors: Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
authors: Masahiro
authors: M
authors: Koyama
authors: Kyoko
authors: K
authors: Naramoto
authors: Takero
authors: T
authors: Nakajima
authors: Toshifumi
authors: T
authors: Aoyama
authors: Masanori
authors: M
authors: Watanabe
authors: Kozo
authors: K
authors: Nakamura
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000302:isolation & purification*; D001794:Blood Pressure / Q000187:drug effects; D019612:Fagopyrum / Q000737:chemistry*; D005285:Fermentation; D048191:Lactiplantibacillus plantarum / Q000378:metabolism; D008297:Male; D010455:Peptides / Q000008:administration & dosage / Q000302:isolation & purification*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D010940:Plant Proteins / Q000008:administration & dosage / Q000302:isolation & purification; D018520:Plant Shoots / Q000737:chemistry*; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D000959:Antihypertensive Agents; D010455:Peptides; D010936:Plant Extracts; D010940:Plant Proteins