title: [Adaptation of an immunohistochemistry protocol for the detection of Leptospira spp. in samples of formaldehyde-fixed tissue].
abstract: The immunohistochemistry technique was evaluated in tissue samples fixed in formaldehyde saline solution 10 % and included in paraffin to be used as a lab method allowing to identify leptospires in tissues. Samples obtained from the experimental inoculation of 8 guinea pigs carriers of L. interrogans Pomona isolated from a clinical case were used. The disease was reproduced in a lab model. The histologic sections of the kidneys of the animals inoculated were subjected to histopathological studies, immunofluorescence, Warthin-Starry stain, and immunohistochemistry technique using formaldehyde-fixed samples. This technique proved to be an efficient tool for the diagnosis of leptospirosis.
authors: Instituto Nacional de Tecnología Agropecuaria, Argentina. fdelgado@cicv.inta.gov.ar
authors: Fernando
authors: F
authors: Delgado
authors: Bibiana
authors: B
authors: Brihuega
authors: Agustín
authors: A
authors: Venzano
authors: Daniel
authors: D
authors: Funes
authors: Francisco
authors: F
authors: Blanco Viera
authors: Carmelo
authors: C
authors: Auteri
authors: Graciela
authors: G
authors: Romero
authors: Félix
authors: F
authors: Capellino
authors: Luis
authors: L
authors: Sarmiento
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D004396:Coloring Agents; D005404:Fixatives / Q000494:pharmacology*; D019085:Fluorescent Antibody Technique, Direct; D005557:Formaldehyde / Q000494:pharmacology*; D006168:Guinea Pigs; D006416:Hematoxylin; D007124:Immunoenzyme Techniques / Q000379:methods*; D007684:Kidney Tubules / Q000187:drug effects / Q000382:microbiology* / Q000648:ultrastructure; D007919:Leptospira / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D007922:Leptospirosis / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D016612:Paraffin Embedding; D016622:Silver Staining; D013194:Staining and Labeling; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D004396:Coloring Agents; D005404:Fixatives; D005557:Formaldehyde; D006416:Hematoxylin