title: Rose Bengal staining - diagnostic aid for potentially malignant and malignant disorders: a pilot study.
abstract: BACKGROUND
  The word "Cancer" itself is sufficient to cause a fear in the minds of people. Early detection of oral potentially malignant and malignant disorders is still a diagnostic challenge for most of the clinicians. In the present study, we have evaluated the efficacy of Rose Bengal staining in the detection of oral premalignant and malignant lesions.
  
  
  OBJECTIVE
  The aim of the study was to evaluate the efficacy of the Rose Bengal staining for the identification of dysplastic areas clinically, and then correlating the findings with the histological grading of dysplasia.
  
  
  METHODS
  A total of 20 patients were selected for the study. Patients had been evaluated clinically and histopathologically along with the grading of dysplasia, and Rose Bengal stain was painted over the lesional mucosa with the patient's consent. Incisional biopsies were taken from the stained area of the Rose Bengal dye, and studied histopathologically. Grading of dysplasia and intensity of the Rose Bengal staining were found to be correlated. Chi square test was performed and a statistical significance of P<0.001 was observed. Statistical significance was defined as P<0.001.
  
  
  CONCLUSIONS
  Grading of dysplasia and the intensity of Rose Bengal staining were directly proportional to each other according to this study. P value was found to be significant. Thus Rose Bengal stain can be used as a diagnostic aid in the detection of oral potentially malignant and malignant disorders.
authors: Department of Oral Pathology & Microbiology, Desh Bhagat Dental College & Hospital, Sri Muktsar Sahib, Punjab, India.
authors: Nitasha
authors: N
authors: Mittal
authors: Sangeeta
authors: S
authors: Palaskar
authors: Madhu
authors: M
authors: Shankari
mesh_terms: D001114:Areca; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000473:pathology; D018289:Carcinoma, Verrucous / Q000175:diagnosis / Q000473:pathology; D002610:Cheek / Q000473:pathology; D055088:Early Detection of Cancer; D005456:Fluorescent Dyes; D006801:Humans; D007972:Leukoplakia, Oral / Q000175:diagnosis / Q000473:pathology; D009060:Mouth Floor / Q000473:pathology; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000473:pathology; D060787:Neoplasm Grading; D010865:Pilot Projects; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000473:pathology; D012395:Rose Bengal; D012680:Sensitivity and Specificity; D014030:Tobacco, Smokeless
chemical_list: D005456:Fluorescent Dyes; D012395:Rose Bengal