title: Toll-like receptor-2 plays a fundamental role in periodontal bacteria-accelerated abdominal aortic aneurysms.
abstract: BACKGROUND
  Periodontopathic bacteria are detected at a high rate in specimens obtained from the aortic walls of patients with abdominal aortic aneurysm (AAA) and are involved in AAA development. The purpose of this study was to clarify the role of Toll-like receptors (TLRs), which are key receptors of virulence factors of many periodontal bacteria, on periodontopathic bacteria-accelerated AAA progression.
  
  
  RESULTS
  AAA was produced by peri-aortic application of 0.25 mol/L CaCl2, with NaCl used as a control. The mice were inoculated with live Porphyromonas (P.) gingivalis or vehicle once weekly. At 4 weeks after the application of CaCl2, the aortic diameter of the P. gingivalis-infected wild-type mice showed a significant increase in comparison with vehicle control mice (P<0.05). The P. gingivalis-infected TLR-2 deficient mice showed no statistical increase in aortic diameter over the same period. The aortic diameter of the P. gingivalis-infected TLR-4 deficient mice statistically increased. Immunohistochemically, the levels of matrix metalloproteinase-2 and -9 in the aneurysmal samples from wild-type mice were higher than in TLR-2 deficient mice.
  
  
  CONCLUSIONS
  P. gingivalis accelerated the progression of experimental AAA through TLR-2 signaling.
authors: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Norio
authors: N
authors: Aoyama
authors: Jun-ichi
authors: J
authors: Suzuki
authors: Masahito
authors: M
authors: Ogawa
authors: Ryo
authors: R
authors: Watanabe
authors: Naho
authors: N
authors: Kobayashi
authors: Tomoya
authors: T
authors: Hanatani
authors: Norihiko
authors: N
authors: Ashigaki
authors: Asuka
authors: A
authors: Sekinishi
authors: Yuichi
authors: Y
authors: Izumi
authors: Mitsuaki
authors: M
authors: Isobe
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016866:Bacteroidaceae Infections / Q000235:genetics / Q000276:immunology / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000276:immunology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D010519:Periodontium / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000276:immunology*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology
chemical_list: C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse