title: Imaging of magnetic microfield distortions allows sensitive single-cell detection.
abstract: Cell tracking with magnetic resonance imaging (MRI) is mostly performed using superparamagnetic iron oxide (SPIO) nanoparticle-labeled cells. However, negative contrast in T2*-weighted imaging is inherently problematic as a homogeneous background signal is required to visualize the negative signal. In a magnetic field, SPIO-labeled cells develop their own magnetization, distorting the main field. We show here a method to visualize these distortions and use them to identify single cells with increased sensitivity and certainty compared to T2* images. We labeled HeLa cells with SPIOs, suspended labeled cells in agarose to make phantoms, and performed high-resolution gradient-echo MRI. Phase images were processed to enhance the visibility of single cells. To quantify SPIO content, we generated a map of frequency differences. MRI of cell phantoms showed that single cells could be detected at concentrations ranging from 200 to 10,000 cells mL(-1). Postprocessing of the magnetic resonance phase images reveals characteristic microfield distortions, increasing dramatically the sensitivity of cell recognition, compared to unprocessed T2* images. Calculating frequency shifts and comparing microfield distortions to simulations permit estimation of the nanoparticle load of single cells. We expect the ability to detect and quantify the iron load of single cells to prove useful in studies of cell trafficking, especially in rare cell populations.
authors: Department of Radiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors: Randall L
authors: RL
authors: Lindquist
authors: Sebastian
authors: S
authors: Papazoglou
authors: Constantin
authors: C
authors: Scharlach
authors: Helmar
authors: H
authors: Waiczies
authors: Jörg
authors: J
authors: Schnorr
authors: Matthias
authors: M
authors: Taupitz
authors: Bernd
authors: B
authors: Hamm
authors: Eyk
authors: E
authors: Schellenberger
mesh_terms: D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*