title: Catalytic characterization of human microsomal glutathione S-transferase 2: identification of rate-limiting steps.
abstract: Microsomal glutathione S-transferase 2 (MGST2) is a 17 kDa trimeric integral membrane protein homologous to leukotriene C4 synthase (LTC4S). MGST2 has been suggested to catalyze the biosynthesis of the pro-inflammatory mediator leukotriene C4 (LTC4) in cells devoid of LTC4S. A detailed biochemical study of MGST2 is critical for the understanding of its cellular function and potential role as an LTC4-producing enzyme. Here we have characterized the substrate specificity and catalytic properties of purified MGST2 by steady-state and pre-steady-state kinetic experiments. In comparison with LTC4S, which has a catalytic efficiency of 8.7 × 10(5) M(-1) s(-1), MGST2, with a catalytic efficiency of 1.8 × 10(4) M(-1) s(-1), is considerably less efficient in producing LTC4. However, the two enzymes display a similar KM(LTA4) of 30-40 μM. While LTC4S has one activated glutathione (GSH) (forming a thiolate) per enzyme monomer, the MGST2 trimer seems to display only third-of-the-sites reactivity for thiolate activation, which in part would explain its lower catalytic efficiency. Furthermore, MGST2 displays GSH-dependent peroxidase activity of ∼0.2 μmol min(-1) mg(-1) toward several lipid hydroperoxides. MGST2, but not LTC4S, is efficient in catalyzing conjugation of the electrophilic substrate 1-chloro-2,4-dinitrobenzene (CDNB) and the lipid peroxidation product 4-hydroxy-2-nonenal with GSH. Using stopped-flow pre-steady-state kinetics, we have characterized the full catalytic reaction of MGST2 with CDNB and GSH as substrates, showing an initial rapid equilibrium binding of GSH followed by thiolate formation. Burst kinetics for the CDNB-GSH conjugation step was observed only at low GSH concentrations (thiolate anion formation becoming rate-limiting under these conditions). Product release is rapid and does not limit the overall reaction. Therefore, in general, the chemical conjugation step is rate-limiting for MGST2 at physiological GSH concentrations. MGST2 and LTC4S exhibit distinct catalytic and mechanistic properties, reflecting adaptation to broad and specific physiological functions, respectively.
authors: Department of Medical Biochemistry and Biophysics, Chemistry II, Karolinska Institutet , Stockholm, Sweden.
authors: Shabbir
authors: S
authors: Ahmad
authors: Damian
authors: D
authors: Niegowski
authors: Anders
authors: A
authors: Wetterholm
authors: Jesper Z
authors: JZ
authors: Haeggström
authors: Ralf
authors: R
authors: Morgenstern
authors: Agnes
authors: A
authors: Rinaldo-Matthis
mesh_terms: D002384:Catalysis; D020134:Catalytic Domain; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D017997:Leukotriene C4 / Q000096:biosynthesis; D011522:Protons; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D011522:Protons; D013438:Sulfhydryl Compounds; D017997:Leukotriene C4; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase; D005978:Glutathione