title: Expression of TRAIL and its receptors DR5 and DcR2 in orthodontic tooth movement.
abstract: TRAIL is a transmembrane protein that induces apoptosis in various tissues including alveolar bone. Its in vitro expression can be activated by several methods, such as RANKL administration and cell scraping. Expression of TRAIL and its receptors DR5 and DcR2 was examined in osteoclast-like cells to analyze their effects on cell lifespan and to explore their role in orthodontic tooth movement.
  
  
  
  Osteoclast-like cells were differentiated from a mouse hematopoietic cell line by stimulation with RANKL for 24 h (T1), 72 h (T2) or 5 days (T3); some cultures were then scraped. Immunostaining for TRAIL, DR5 and DcR2 was evaluated by immunocytochemistry and Western blot analysis in control and treated cells.
  
  
  
  Significantly greater TRAIL expression was found in treated osteoclast-like cells at T1 and T3 both on immunocytochemistry and Western blotting. TRAIL expression peaked at T1 and T3 in correspondence with DcR2 and DR5 maxima, respectively.
  
  
  
  These data may contribute to a better understanding of the mechanisms regulating tooth movement and to improve the accuracy of orthodontic treatments.
authors: Department of Bio-Medical Sciences, Physiology Section, University of Catania, Catania, Italy.
authors: V
authors: V
authors: Cardile
authors: G
authors: G
authors: Musumeci
authors: E
authors: E
authors: Sicurezza
authors: S
authors: S
authors: Caggia
authors: M C
authors: MC
authors: Rusu
authors: R
authors: R
authors: Leonardi
authors: C
authors: C
authors: Loreto
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D009970:Orthodontics; D010010:Osteoclasts / Q000378:metabolism*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism*; D014087:Tooth Movement Techniques
chemical_list: D008562:Membrane Glycoproteins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand; C505640:Tnfrsf10b protein, mouse; C505654:Tnfsf10 protein, mouse