title: Solution-state 2D NMR spectroscopy of plant cell walls enabled by a dimethylsulfoxide-d6/1-ethyl-3-methylimidazolium acetate solvent.
abstract: Lignocellulosic biomass is composed of the polysaccharides cellulose and hemicellulose and the polyphenol lignin. Many current methods for analyzing the structure of lignocelluloses involve a sequential extraction of the material and subsequent analysis of the resulting fractions, which is labor-intensive and time-consuming. The work presented here assesses the dissolution of whole lignocellulosic material, focusing on biomass derived from the perennial bioenergy grass Miscanthus. The solvent dimethylsulfoxide (DMSO)-d6 containing 1-ethyl-3-methylimidazolium acetate ([Emim]OAc) was able to dissolve lignocellulosic material completely and gave high-resolution 2D heteronuclear single quantum coherence (HSQC) NMR spectra of the entire array of wall polymers. Extrapolated time-zero HSQC was applied using DMSO-d6/[Emim]OAc-d14 and enabled quantitative analysis of structural traits of lignocellulose components.
authors: Energy Biosciences Institute, University of California, Berkeley, California 94720, United States.
authors: Kun
authors: K
authors: Cheng
authors: Hagit
authors: H
authors: Sorek
authors: Herbert
authors: H
authors: Zimmermann
authors: David E
authors: DE
authors: Wemmer
authors: Markus
authors: M
authors: Pauly
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D008031:Lignin / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D059828:Plant Cells / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D012997:Solvents; C036909:lignocellulose; D008031:Lignin; C518739:1-ethyl-3-methylimidazolium; D004121:Dimethyl Sulfoxide