title: Advancing nematode barcoding: a primer cocktail for the cytochrome c oxidase subunit I gene from vertebrate parasitic nematodes.
abstract: Although nematodes are one of the most diverse metazoan phyla, species identification through morphology is difficult. Several genetic markers have been used for their identification, but most do not provide species-level resolution in all groups, and those that do lack primer sets effective across the phylum, precluding high-throughput processing. This study describes a cocktail of three novel primer pairs that overcome this limitation by recovering cytochrome c oxidase I (COI) barcodes from diverse nematode lineages parasitic on vertebrates, including members of three orders and eight families. Its effectiveness across a broad range of nematodes enables high-throughput processing.
authors: Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors: Sean W J
authors: SW
authors: Prosser
authors: Maria G
authors: MG
authors: Velarde-Aguilar
authors: Virginia
authors: V
authors: León-Règagnon
authors: Paul D N
authors: PD
authors: Hebert
mesh_terms: D000818:Animals; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000145:classification / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D009348:Nematoda / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D014714:Vertebrates / Q000469:parasitology
chemical_list: D003576:Electron Transport Complex IV
keywords: barcoding; identification; nematodes; primers