title: Speed-stability paradox in DNA-scanning by zinc-finger proteins.
abstract: Extensive contact with DNA via multiple zinc fingers allows highly specific DNA-binding of zinc-finger-class transcription factors, but can also slow the target search process. Here we introduce recent insights into how zinc-finger proteins can rapidly scan DNA. Potential application of the new knowledge to the zinc-finger-based technology is also discussed.
authors: Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, USA. j.iwahara@utmb.edu
authors: Junji
authors: J
authors: Iwahara
authors: Yaakov
authors: Y
authors: Levy
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D006801:Humans; D059372:Nucleotide Motifs; D011485:Protein Binding; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D014157:Transcription Factors; D004247:DNA
keywords: DNA-scanning; dynamics; gene control; kinetics; target search; zinc-finger nucleases; zinc-finger transcription factors