title: FAS and FAS-L genotype and expression in patients with recurrent pregnancy loss.
abstract: We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case-control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules.
authors: Obstetrics Department, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Priscilla Chamelete Andrade
authors: PC
authors: Banzato
authors: Silvia
authors: S
authors: Daher
authors: Evelyn
authors: E
authors: Traina
authors: Maria Regina
authors: MR
authors: Torloni
authors: Bárbara Yasmin
authors: BY
authors: Gueuvoghlanian-Silva
authors: Renata Fiorini
authors: RF
authors: Puccini
authors: Karen Priscilla Tezotto
authors: KP
authors: Pendeloski
authors: Rosiane
authors: R
authors: Mattar
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D001938:Brazil; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D010641:Phenotype; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult; D019014:fas Receptor / Q000235:genetics*
chemical_list: C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D012333:RNA, Messenger; D019014:fas Receptor
keywords: FAS; FAS-L; apoptosis; polymorphism; recurrent pregnancy loss