title: TALEN-mediated precise genome modification by homologous recombination in zebrafish.
abstract: We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator-like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.
authors: Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing, China.
authors: Yao
authors: Y
authors: Zu
authors: Xiangjun
authors: X
authors: Tong
authors: Zhanxiang
authors: Z
authors: Wang
authors: Da
authors: D
authors: Liu
authors: Ruochuan
authors: R
authors: Pan
authors: Zhe
authors: Z
authors: Li
authors: Yingying
authors: Y
authors: Hu
authors: Zhou
authors: Z
authors: Luo
authors: Peng
authors: P
authors: Huang
authors: Qian
authors: Q
authors: Wu
authors: Zuoyan
authors: Z
authors: Zhu
authors: Bo
authors: B
authors: Zhang
authors: Shuo
authors: S
authors: Lin
mesh_terms: D000818:Animals; D001483:Base Sequence; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018390:Gene Targeting / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D023281:Genomics; D012313:RNA; D011995:Recombination, Genetic / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D012313:RNA; D014446:Tyrosine 3-Monooxygenase; D003851:Deoxyribonucleases