title: The lecticans of mammalian brain perineural net are O-mannosylated.
abstract: O-Mannosylation is an important protein modification in brain. During the last years, a few mammalian proteins have been identified as targets of the protein-O-mannosyltransferases 1 and 2. However, these still cannot explain the high content of O-mannosyl glycans in brain and the strong brain involvement of congenital muscular dystrophies caused by POMT mutations (Walker-Warburg syndrome, dystroglycanopathies). By fractionating and analyzing the glycoproteome of mouse and calf brain lysates, we could show that proteins of the perineural net, the lecticans, are O-mannosylated, indicating that major components of neuronal extracellular matrix are O-mannosylated in mammalian brain. This finding corresponds with the high content of O-mannosyl glycans in brain as well as with the brain involvement of dystroglycanopathies. In contrast, the lectican neurocan is not O-mannosylated when recombinantly expressed in EBNA-293 cells, revealing the possibility of different control mechanisms for the initiation of O-mannosylation in different cell types.
authors: Institute of Biochemistry II, Medical Faculty, University of Cologne , Köln, Germany.
authors: Sandra
authors: S
authors: Pacharra
authors: Franz-Georg
authors: FG
authors: Hanisch
authors: Martina
authors: M
authors: Mühlenhoff
authors: Andreas
authors: A
authors: Faissner
authors: Uwe
authors: U
authors: Rauch
authors: Isabelle
authors: I
authors: Breloy
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002417:Cattle; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D009415:Nerve Net / Q000378:metabolism*; D058580:Neurocan; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D006023:Glycoproteins; C539091:Ncan protein, rat; D058580:Neurocan; D011134:Polysaccharides; D011994:Recombinant Proteins