title: Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics.
abstract: Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 °C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.
authors: School of Chemistry, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, United Kingdom. A.J.Wilson@leeds.ac.uk
authors: Andrew J
authors: AJ
authors: Wilson
authors: James R
authors: JR
authors: Ault
authors: Maria H
authors: MH
authors: Filby
authors: Hazel I A
authors: HI
authors: Philips
authors: Alison E
authors: AE
authors: Ashcroft
authors: Nicholas C
authors: NC
authors: Fletcher
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D045304:Cytochromes c / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006321:Heart; D006736:Horses; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D055550:Protein Stability; D012428:Ruthenium / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D009942:Organometallic Compounds; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D045304:Cytochromes c