title: NFP, a LysM protein controlling Nod factor perception, also intervenes in Medicago truncatula resistance to pathogens.
abstract: Plant LysM proteins control the perception of microbial-derived N-acetylglucosamine compounds for the establishment of symbiosis or activation of plant immunity. This raises questions about how plants, and notably legumes, can differentiate friends and foes using similar molecular actors and whether any receptors can intervene in both symbiosis and resistance. To study this question, nfp and lyk3 LysM-receptor like kinase mutants of Medicago truncatula that are affected in the early steps of nodulation, were analysed following inoculation with Aphanomyces euteiches, a root oomycete. The role of NFP in this interaction was further analysed by overexpression of NFP and by transcriptome analyses. nfp, but not lyk3, mutants were significantly more susceptible than wildtype plants to A. euteiches, whereas NFP overexpression increased resistance. Transcriptome analyses on A. euteiches inoculation showed that mutation in the NFP gene led to significant changes in the expression of c. 500 genes, notably involved in cell dynamic processes previously associated with resistance to pathogen penetration. nfp mutants also showed an increased susceptibility to the fungus Colletotrichum trifolii. These results demonstrate that NFP intervenes in M. truncatula immunity, suggesting an unsuspected role for NFP in the perception of pathogenic signals.
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.|CNRS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.
authors: Thomas
authors: T
authors: Rey
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.|CNRS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.
authors: Amaury
authors: A
authors: Nars
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.|CNRS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.
authors: Maxime
authors: M
authors: Bonhomme
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.|CNRS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.
authors: Arnaud
authors: A
authors: Bottin
authors: Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165, Université d'Evry Val d'Essonne, ERL CNRS 8196, CP 5708, F-91057, Evry Cedex, France.
authors: Stéphanie
authors: S
authors: Huguet
authors: Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165, Université d'Evry Val d'Essonne, ERL CNRS 8196, CP 5708, F-91057, Evry Cedex, France.
authors: Sandrine
authors: S
authors: Balzergue
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.
authors: Marie-Françoise
authors: MF
authors: Jardinaud
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.
authors: Jean-Jacques
authors: JJ
authors: Bono
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.
authors: Julie
authors: J
authors: Cullimore
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.|CNRS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.
authors: Bernard
authors: B
authors: Dumas
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.
authors: Clare
authors: C
authors: Gough
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.|CNRS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France.
authors: Christophe
authors: C
authors: Jacquet
mesh_terms: D044744:Aphanomyces / Q000472:pathogenicity / Q000502:physiology; D020231:Colletotrichum / Q000472:pathogenicity*; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D046913:Medicago truncatula / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis / Q000502:physiology
chemical_list: D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases