title: Trichothecene genotypes of Fusarium graminearum from wheat in Uruguay.
abstract: Gibberella zeae (Schwein.) Petch (anamorph F. graminearum Schwabe) is the primary causal agent of FHB of wheat in Uruguay. In the last decade, F. graminearum has produced destructive epidemics on wheat in Uruguay, causing yield losses and price discounts due to reduced seed quality. Strains of F. graminearum clade usually express one of three strain-specific profiles of trichothecene metabolites: nivalenol and its acetylated derivatives (NIV chemotype), deoxynivalenol and 3-acetyldeoxynivalenol (3-AcDON chemotype), or deoxynivalenol and 15-acetyldeoxynivalenol (15-AcDON chemotype). A multiplex PCR assay of Tri3, Tri5, and Tri7 was used to determine the trichothecene genotype of 111 strains of F. graminearum collected during 2003 and 2009 growing seasons from fields located in the major wheat production area of Uruguay. The result showed that all except one of the isolates were of DON genotype, with the remainder of NIV genotype in years 2003 and 2009. All strains with the DON genotype were also of the 15-AcDON genotype in 2003 and nearly all (45/50) in 2009. No DON/3-AcDON genotypes were found in either growing season. No potential shifts in the populations were found in the trichothecene genotypes between 2003 and the 2009 epidemic FHB harvest seasons. This study provides the first data on trichothecene genotypes of F. graminearum strains isolated from wheat in Uruguay and add to the current regional knowledge of trichothecene genotypes.
authors: Laboratorio de Micología, Facultad de Ciencias-Facultad de Ingeniería, UdelaR, Julio Herrera y Reissig 565, 11200 Montevideo, Uruguay. dpan@fing.edu.uy
authors: Dinorah
authors: D
authors: Pan
authors: Natalia
authors: N
authors: Calero
authors: Ana
authors: A
authors: Mionetto
authors: Lina
authors: L
authors: Bettucci
mesh_terms: D005516:Food Microbiology; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification; D005800:Genes, Fungal / Q000235:genetics; D005838:Genotype; D060885:Multiplex Polymerase Chain Reaction; D012621:Seasons; D014255:Trichothecenes / Q000235:genetics*; D014908:Triticum / Q000382:microbiology*; D014583:Uruguay
chemical_list: D014255:Trichothecenes