title: Quantification of vitamin D3 and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.).
abstract: Changes in vitamin D(3) and its metabolites were investigated following UVB- and heat-treatment in the leaves of Solanum glaucophyllum Desf., Solanum lycopersicum L. and Capsicum annuum L. The analytical method used was a sensitive and selective liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method including Diels-Alder derivatisation. Vitamin D(3) and 25-hydroxy vitamin D(3) were found in the leaves of all plants after UVB-treatment. S. glaucophyllum had the highest content, 200 ng vitamin D(3)/g dry weight and 31 ng 25-hydroxy vitamin D(3)/g dry weight, and was the only plant that also contained 1,25 dihydroxy vitamin D(3) in both free (32 ng/g dry weight) and glycosylated form (17 ng/g dry weight).
authors: Division of Food Chemistry, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark. riba@food.dtu.dk
authors: Rie Bak
authors: RB
authors: Jäpelt
authors: Daniele
authors: D
authors: Silvestro
authors: Jørn
authors: J
authors: Smedsgaard
authors: Poul Erik
authors: PE
authors: Jensen
authors: Jette
authors: J
authors: Jakobsen
mesh_terms: D002212:Capsicum / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D002762:Cholecalciferol / Q000032:analysis* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D036061:Solanum glaucophyllum / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D002762:Cholecalciferol