title: [Large-scale purification and acute toxicity of cowpea trypsin inhibitor].
abstract: OBJECTIVE
  To provide the acute toxicity data of cowpea trypsin inhibitor (CpTI) using recombinant protein purified from E. coli.
  
  
  METHODS
  Recombinant CpTI protein was expressed and purified from E. coli. Bacterial recombinant plasmid was transformed into E. coli and the transformed cells were induced with IPTG. The expressed CpTI protein was purified by hydrophobic interaction chromatography and anion exchange chromatography. Sixty mice, randomly assigned to 6 groups, were administrated 10.0, 4.64, 2.15 and 1.00 g/kg BW of CpTI or 5.00 g/kg BW of BSA control protein or sterile water respectively by oral gavage.
  
  
  RESULTS
  All animals survived with no significant change in body weight and food consumption throughout the study. Macroscopic necropsy examination on day 15 revealed no gross pathological lesions in any of the animals. The maximum tolerated dose (MTD) of CpTI was more than 10.0 g/kg body weight in mice.
  
  
  CONCLUSIONS
  No toxicity of CpTI protein was found in ICR mice model.
authors: Key Laboratory of Trace Element Nutrition MOH, Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors: Zhaolong
authors: Z
authors: Gong
authors: Qin
authors: Q
authors: Zhuo
authors: Min
authors: M
authors: Zhang
authors: Jinghuan
authors: J
authors: Wu
authors: Jianhua
authors: J
authors: Piao
authors: Xiaoguang
authors: X
authors: Yang
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity*; D023382:Toxicity Tests, Acute; D014361:Trypsin Inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity*
chemical_list: D011994:Recombinant Proteins; D014361:Trypsin Inhibitors