title: The role of uncoupling protein 2 and 3 genes polymorphism and energy expenditure in obese Indonesian children.
abstract: OBJECTIVE
  Uncoupling protein (UCP) genes, which may contribute to energy metabolism in mitochondria, may be involved in the pathogenesis of obesity. We analyzed the differences in energy expenditure between single nucleotide polymorphisms (SNPs) UCP3-55C/T, UCP3 Y210Y, and UCP2 A55V among Indonesian children.
  
  
  METHODS
  The study included 76 schoolchildren (36 obese and 40 healthy; mean age, 12.8 years) in Semarang, Indonesia. Body composition was measured by bioelectrical impedance analysis; resting energy expenditure (REE) by indirect calorimetry; physical activity by uniaxial accelerometer; and total energy expenditure (TEE) by the equations extrapolated from REE and physical activity. UCP3-55C/T, UCP3 Y210Y, and UCP2 A55V were examined by restriction length fragment polymorphism analysis.
  
  
  RESULTS
  The TEE of the subjects with the T/T genotype at UCP3-55C/T after adjusting for fat-free mass (63.2±7.2 kcal/kg/day) and T/T at UCP2 A55V (62.8±5.6 kcal/kg/day) was lower than that of the subjects with the C/C and C/T genotypes (p<0.05). The REE of the subjects with these T/T genotypes tended to be lower than that of the subjects with C/C and C/T (p≥0.05). No significant differences in REE or TEE were found between the UCP3 Y210Y genotypes.
  
  
  CONCLUSIONS
  The subjects with the T/T genotypes of UCP3-55C/T or UCP2 A55V had lower TEE than those with other genotypes.
authors: Department of Pediatrics, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Dr. Sutomo 16-18 Semarang Central Java 50231, Semarang, Indonesia. maria_mexitalia@yahoo.com
authors: Maria
authors: M
authors: Mexitalia
authors: Taro
authors: T
authors: Yamauchi
authors: Agustini
authors: A
authors: Utari
authors: Damayanti R
authors: DR
authors: Sjarif
authors: Hertanto W
authors: HW
authors: Subagio
authors: Agustinus
authors: A
authors: Soemantri
authors: Takafumi
authors: T
authors: Ishida
mesh_terms: D000293:Adolescent; D002648:Child; D017097:Electric Impedance; D004734:Energy Metabolism / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007214:Indonesia; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics*; D009765:Obesity / Q000235:genetics* / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; C000604392:UCP3 protein, human; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3