title: Pharmacokinetic study of multiple active constituents after oral gavage of Guizhi decoction in rats using a LC-MS/MS method.
abstract: Guizhi decoction (GZD) is a classic traditional Chinese medicine formula, clinically used for the treatment of influenza, common cold, and other pyretic conditions. A sensitive, specific, and validated liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to investigate the pharmacokinetic properties of cinnamic acid, hippuric acid, paeoniflorin, and glycyrrhetic acid in rat. After single dose oral administration of 7.9 g extract/kg body weight GZD in rats, plasma concentrations of cinnamic acid, hippuric acid, paeoniflorin, and glycyrrhetic acid were measured by LC-MS/MS. Pharmacokinetic parameters were calculated from the plasma concentration-time data. The values of AUC0-t, half-life (t 1/2), and C max were 7.2 ± 2.3 μg h/mL, 1.2 ± 0.3 h, and 9.2 ± 5.2 μg/mL for cinnamic acid, 53 ± 31 μg h/mL, 2.8 ± 2.0 h, and 17 ± 3 μg/mL for hippuric acid, 1.1 ± 0.5 μg h/mL, 1.9 ± 1.1 h, and 0.6 ± 0.3 μg/mL for paeoniflorin, and 11 ± 6 μg h/mL, 6.6 ± 2.5 h, and 0.9 ± 0.6 μg/mL for glycyrrhetic acid, respectively. The results would offer useful information for effective components of GZD in vivo.
authors: Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, People's Republic of China.
authors: Yingrong
authors: Y
authors: Chen
authors: Chenglu
authors: C
authors: Gao
authors: Yueming
authors: Y
authors: Ma
authors: Furong
authors: F
authors: Qiu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001565:Benzoates / Q000008:administration & dosage / Q000493:pharmacokinetics; D001952:Bridged-Ring Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000008:administration & dosage / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005615:Freezing; D005960:Glucosides / Q000008:administration & dosage / Q000493:pharmacokinetics; D006034:Glycyrrhetinic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics; D006207:Half-Life; D006626:Hippurates / Q000008:administration & dosage / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D039821:Monoterpenes; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D002934:Cinnamates; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006034:Glycyrrhetinic Acid; D006626:Hippurates; D007202:Indicators and Reagents; D039821:Monoterpenes; D010936:Plant Extracts; C528663:guizhi decoction; C029010:cinnamic acid; C015423:peoniflorin; D006027:Glycosides; C030514:hippuric acid