title: Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina.
abstract: A white rot fungus, identified as Armillaria gemina SKU2114 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose-degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase, and β-glucosidase activities of 146, 34, and 15 U/mL, respectively, and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1270, 0.16, 57, and 0.31 U/mL, respectively, when grown with rice straw as a carbon source. Among various plant biomasses tested for saccharification, aspen biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize the hydrolysis of aspen biomass to achieve the highest level of sugar production. A maximum saccharification yield of 62% (429 mg/g-substrate) was obtained using Populus tomentiglandulosa biomass after 48 h of hydrolysis. A. gemina was shown to be a good option for use in the production of reducing sugars from lignocellulosic biomass.
authors: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Sujit Sadashiv
authors: SS
authors: Jagtap
authors: Saurabh Sudha
authors: SS
authors: Dhiman
authors: Tae-Su
authors: TS
authors: Kim
authors: Jinglin
authors: J
authors: Li
authors: Jung-Kul
authors: JK
authors: Lee
authors: Yun Chan
authors: YC
authors: Kang
mesh_terms: D000468:Alkalies / Q000494:pharmacology; D055392:Armillaria / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002244:Carbon / Q000494:pharmacology; D002480:Cellulase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D032107:Populus / Q000737:chemistry*; D015203:Reproducibility of Results; D013379:Substrate Specificity / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000468:Alkalies; D013501:Surface-Active Agents; C036909:lignocellulose; D002244:Carbon; D008031:Lignin; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases