title: In vitro α-glucosidase inhibition and antioxidative potential of an endophyte species (Streptomyces sp. loyola UGC) isolated from Datura stramonium L.
abstract: Endophytic actinomycetes isolated from Datura stramonium L. was evaluated for its effects against in vitro α-glucosidase inhibition, antioxidant, and free radical scavenging activities. Based on microbial cultural characteristic and 16S rRNA sequencing, it was identified as Streptomyces sp. loyola UGC. The methanolic extract of endophytic actinomycetes (MeEA) shows remarkable inhibition of α-glucosidase (IC50 730.21 ± 1.33 μg/ml), scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC50 435.31 ± 1.79 μg/ml), hydroxyl radical (IC50 350.21 ± 1.02 μg/ml), nitric oxide scavenging (IC50 800.12 ± 1.05 μg/ml), superoxide anion radical (IC50 220.31 ± 1.47 μg/ml), as well as a high and dose-dependent reducing power. The MeEA also showed a strong suppressive effect on rat liver lipid peroxidation. Antioxidants of β-carotene linoleate model system revels significantly lower than BHA. The total phenolic content of the extract was 176 mg of catechol equivalents/gram extract. Perusal of this study indicates MeEA can be used as natural resource of α-glucosidase inhibitor and antioxidants.
authors: Department of Plant Biology and Biotechnology, Loyola College, Chennai 600 034, India.
authors: I V S
authors: IV
authors: Nimal Christhudas
authors: P
authors: P
authors: Praveen Kumar
authors: P
authors: P
authors: Agastian
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D013286:Datura stramonium / Q000382:microbiology*; D060026:Endophytes / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D065089:Glycoside Hydrolase Inhibitors; D012336:RNA, Ribosomal, 16S