title: An efficient approach to identify Ginkgo biloba cultivars  by using random amplified polymorphic DNA markers with a manual cultivar  identification diagram strategy.
abstract: Cultivar identification is a key step to avoid the  formation of homonyms and synonyms of Ginkgo biloba. In this study, a  new approach based on combinational utilization of polymorphic bands produced  from 6 different random amplified polymorphic DNA (RAPD) primers was developed  for identifying 42 Ginkgo cultivars, and a manual cultivar  identification diagram that consisted of polymorphic bands produced from  different RAPD primers was reported. To check the reliability and efficiency of  the cultivar identification diagram, 5 randomly chosen cultivars were further  tested, and the workability of the diagram was verified. This new approach will  be very helpful for Ginkgo cultivar discrimination and protection, and  will also be beneficial for the nursery industry for early identification of Ginkgo seedlings.
authors: College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, China.
authors: G P
authors: GP
authors: Li
authors: C Q
authors: CQ
authors: Zhang
authors: F L
authors: FL
authors: Cao
mesh_terms: D017931:DNA Primers / Q000737:chemistry; D018744:DNA, Plant / Q000737:chemistry* / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D020441:Ginkgo biloba / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers