title: Proteomic analysis of the aqueous humor in patients with wet age-related macular degeneration.
abstract: OBJECTIVE
  A number of studies have shown that the levels of some proteins in the aqueous humor (AH) are altered and correlate with the mechanisms or prognosis of many eye diseases. To identify the possible mechanisms that lead to the development of wet age-related macular degeneration (AMD), a proteomic analysis of the AH composition from wet AMD patients was performed and compared with that from non-AMD cataract patients.
  
  
  METHODS
  Six wet AMD and six non-AMD cataract patients were enrolled. A proteomic approach which included two-dimensional electrophoresis coupled with MS and bioinformatics methods were used to identify AH proteins with altered expression in wet AMD compared with non-AMD patients. An ELISA was used to validate the proteomic results.
  
  
  RESULTS
  We separated 78 protein spots and identified 68 that were differently expressed in the wet AMD group and controls. Numerous proteins identified in this study are implicated in inflammation, apoptosis, angiogenesis, and oxidative stress.
  
  
  CONCLUSIONS
  The AH protein composition was significantly different between wet AMD and non-AMD patients. The proteins identified in this study may be potential biomarkers of wet AMD development and might play a role in the mechanisms of wet AMD.
authors: Department of Ophthalmology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, PR China.
authors: Jiaqi
authors: J
authors: Yao
authors: Xiaoyi
authors: X
authors: Liu
authors: Qin
authors: Q
authors: Yang
authors: Min
authors: M
authors: Zhuang
authors: Feng
authors: F
authors: Wang
authors: Xi
authors: X
authors: Chen
authors: Hui
authors: H
authors: Hang
authors: Weiwei
authors: W
authors: Zhang
authors: Qinghuai
authors: Q
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D016022:Case-Control Studies; D003459:Crystallins / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D063990:Gene Ontology; D006801:Humans; D054835:Lipocalin 1 / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D057135:Wet Macular Degeneration / Q000235:genetics / Q000378:metabolism*
chemical_list: C511246:CRYAA protein, human; D003459:Crystallins; C518443:LCN1 protein, human; D054835:Lipocalin 1
keywords: Aqueous humor; MALDI-TOF MS/MS; Wet age-related macular degeneration