title: Towards a 3D culture of mouse ovarian follicles.
abstract: The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tightly connected follicle cells that mediate the action of external signals and nourish the gamete during its maturation. Thus, the maintenance of follicle organization during the whole growth process is crucial for the correct acquisition of developmental competence. In recent years, much attention has been given to in vitro culture systems capable of maintaining follicle architecture. With the aim of providing a quick reference guide, in this review we will summarize the techniques developed for the 3D culture of mouse follicles.
authors: Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie Lazzaro Spallanzani, Università degli Studi di Pavia, Pavia, Italy.
authors: Martina
authors: M
authors: Belli
authors: Giulia
authors: G
authors: Vigone
authors: Valeria
authors: V
authors: Merico
authors: Carlo Alberto
authors: CA
authors: Redi
authors: Maurizio
authors: M
authors: Zuccotti
authors: Silvia
authors: S
authors: Garagna
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007797:Laminin; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D011509:Proteoglycans
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen