title: Determination of buprenorphine, fentanyl and LSD in whole blood by UPLC-MS-MS.
abstract: A sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method has been developed and validated for the quantification of buprenorphine, fentanyl and lysergic acid diethylamide (LSD) in whole blood. Sample preparation was performed by liquid-liquid extraction (LLE) with methyl tert-butyl ether. UPLC-MS-MS analysis was performed with a mobile phase consisting of ammonium formate (pH 10.2) and methanol. Positive electrospray ionization MS-MS detection was performed with two multiple reaction monitoring transitions for each of the analytes and the deuterium labeled internal standards. Limit of detection values of buprenorphine, fentanyl and LSD were 0.28, 0.044 and 0.0097 ng/mL and limit of quantification values were 0.94, 0.14 and 0.036 ng/mL, respectively. Most phospholipids were removed during LLE. No or only minor matrix effects were observed. The method has been routinely used at the Norwegian Institute of Public Health since September 2011 for qualitative and quantitative detections of buprenorphine, fentanyl and/or LSD in more than 400 whole blood samples with two replicates per sample.
authors: Division of Forensic Medicine and Drug Abuse Research, Norwegian Institute of Public Health, Oslo, Norway. thomas.berg@fhi.no
authors: Thomas
authors: T
authors: Berg
authors: Benedicte
authors: B
authors: Jørgenrud
authors: Dag Helge
authors: DH
authors: Strand
mesh_terms: D000641:Ammonia / Q000737:chemistry; D000701:Analgesics, Opioid / Q000097:blood*; D002047:Buprenorphine / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000592:standards; D004355:Drug Stability; D005283:Fentanyl / Q000097:blood*; D053593:Forensic Toxicology / Q000379:methods* / Q000592:standards; D005561:Formates / Q000737:chemistry; D006213:Hallucinogens / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D008238:Lysergic Acid Diethylamide / Q000097:blood*; D008738:Methyl Ethers / Q000737:chemistry; D009664:Norway; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D015813:Substance Abuse Detection / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D000701:Analgesics, Opioid; D005561:Formates; D006213:Hallucinogens; D008738:Methyl Ethers; C030544:formic acid; C043243:methyl tert-butyl ether; D002047:Buprenorphine; D000641:Ammonia; D008238:Lysergic Acid Diethylamide; D005283:Fentanyl