title: Simultaneous determination of 2-naphthol and 1-hydroxypyrene in fish and shellfish contaminated with crude oil by gas chromatography-mass spectrometry.
abstract: This paper describes a gas chromatography-mass spectrometric method of l-hydroxypyrene (1-HOP) and 2-naphthol (2-NAP) in fish and shellfish. Alkali hydrolysis method in this study was chosen and optimized to the reaction condition for 90 min at 90°C in a 2.0M KOH solution. For five independent determinations at 0.2 and 1.0 μg/kg, the coefficient of variation was less than 5.1%. This method was used to assess the long-term influence of spilled crude oil on marine ecosystems and analyze fifty-two shellfish samples taken in the near of the accident region of the Hebei Spirit oil spill. 2-NAP and 1-HOP were detected in the mean concentration range of 0.09-12.42 and 0.03-0.06 μg/kg, respectively. 2-NAP was detected in a high concentration range in shellfishes gathered in 2 months after the accident and it decreased rapidly to 6 months after that. The results showed that 2-NAP might be an important biomarker in biota contaminated with crude oil.
authors: Department of Environmental Science, Kongju National University, Kongju 314-701, Republic of Korea.
authors: Hyun-Hee
authors: HH
authors: Lim
authors: Ho-Sang
authors: HS
authors: Shin
mesh_terms: D000818:Animals; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008974:Mollusca / Q000737:chemistry; D009284:Naphthols / Q000032:analysis*; D010578:Petroleum / Q000032:analysis*; D059392:Petroleum Pollution / Q000032:analysis; D011721:Pyrenes / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D012758:Shellfish / Q000032:analysis
chemical_list: D009284:Naphthols; D010578:Petroleum; D011721:Pyrenes; C033146:1-hydroxypyrene; C028405:2-naphthol