title: Lypopolysaccharide downregulates the expression of selected phospholipase C genes in cultured endothelial cells.
abstract: The signaling system of phosphoinositides (PI) is involved in a variety of cell and tissue functions, including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation, and cell and tissue polarity. Recently, PI and related molecules, such as the phosphoinositide-specific phospholipases C (PI-PLCs), main players in PI signaling were supposed to be involved in inflammation. Besides the control of calcium levels, PI-PLCs contribute to the regulation of phosphatydil-inositol bisphosphate metabolism, crucial in cytoskeletal organization. The expression of PI-PLCs is strictly tissue specific and evidences suggest that it varies under different conditions, such as tumor progression or cell activation. In a previous study, we obtained a complete panel of expression of PI-PLC isoforms in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for endothelial cells. In the present study, we analyzed the mRNA concentration of PI-PLCs in lipopolysaccharide (LPS)-treated HUVEC by using the multiliquid bioanalyzer methodology after 3, 6, 24, 48, and 72 h from LPS administration. Marked differences in the expression of most PI-PLC codifying genes were evident.
authors: Organi di Senso Department, Faculty of Medicina e Odontoiatria,, Sapienza University of Rome, viale del Policlinico 155, 00185 Rome, Italy. ritalovasco@hotmail.it
authors: V R
authors: VR
authors: Lo Vasco
authors: M
authors: M
authors: Leopizzi
authors: C
authors: C
authors: Chiappetta
authors: C
authors: C
authors: Puggioni
authors: C
authors: C
authors: Della Rocca
authors: P
authors: P
authors: Polonia
authors: R
authors: R
authors: Businaro
mesh_terms: D002460:Cell Line; D015536:Down-Regulation; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D061307:Human Umbilical Vein Endothelial Cells / Q000201:enzymology; D006801:Humans; D007249:Inflammation / Q000139:chemically induced; D008070:Lipopolysaccharides / Q000276:immunology*; D010716:Phosphatidylinositols / Q000276:immunology; D054801:Phosphoinositide Phospholipase C / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction
chemical_list: D008070:Lipopolysaccharides; D010716:Phosphatidylinositols; D012333:RNA, Messenger; D054801:Phosphoinositide Phospholipase C