title: Thermally responsive silicon nanowire arrays for native/denatured-protein separation.
abstract: We present our findings of the selective adsorption of native and denatured proteins onto thermally responsive, native-protein resistant poly(N-isopropylacrylamide) (PNIPAAm) decorated silicon nanowire arrays (SiNWAs). The PNIPAAm-SiNWAs surface, which shows very low levels of native-protein adsorption, favors the adsorption of denatured proteins. The amount of denatured-protein adsorption is higher at temperatures above the lower critical solution temperature (LCST) of PNIPAAm. Temperature cycling surrounding the LCST, which ensures against thermal denaturation of native proteins, further increases the amount of denatured-protein adsorption. Moreover, the PNIPAAm-SiNWAs surface is able to selectively adsorb denatured protein even from mixtures of different protein species; meanwhile, the amount of native proteins in solution is kept nearly at its original level. It is believed that these results will not only enrich current understanding of protein interactions with PNIPAAm-modified SiNWAs surfaces, but may also stimulate applications of PNIPAAm-SiNWAs surfaces for native/denatured protein separation.
authors: Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou, 215123, People's Republic of China.
authors: Hongwei
authors: H
authors: Wang
authors: Yanwei
authors: Y
authors: Wang
authors: Lin
authors: L
authors: Yuan
authors: Lei
authors: L
authors: Wang
authors: Weikang
authors: W
authors: Yang
authors: Zhaoqiang
authors: Z
authors: Wu
authors: Dan
authors: D
authors: Li
authors: Hong
authors: H
authors: Chen
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins; D000327:Adsorption; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009113:Muramidase / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D053770:Nanowires / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D011506:Proteins; C052970:poly-N-isopropylacrylamide; D009113:Muramidase; D012825:Silicon