title: MUC1 and Toll-like receptor-2 expression in burning mouth syndrome and oral lichen planus.
abstract: OBJECTIVE
  Expression levels of MUC1 and TLR-2 were evaluated in burning mouth syndrome (BMS) patients and compared with those of controls and oral lichen planus (OLP) patients. The relationships between the expression levels of MUC1 and TLR-2 and levels of salivary pro-inflammatory cytokines were also investigated.
  
  
  METHODS
  Ten female BMS and ten female OLP patients were included. Ten female age-matched volunteers served as controls. RNA was isolated from stimulated whole saliva samples. Real-time PCR was used to quantify MUC1 and TLR-2 mRNA levels relative to β-actin and GAPDH mRNA levels. The clarified supernatants of saliva samples were used to measure IL-1β, IL-6, IL-8, and TNF-α levels. The level of blood contamination in saliva samples was also determined.
  
  
  RESULTS
  There were significant increases in MUC1 transcripts in BMS patients compared with OLP patients (1.766-fold) as well as controls (1.840-fold). There was no significant difference in TLR-2 expression among the groups. The OLP patients showed significantly higher levels of IL-6 and blood contamination in saliva than other groups. The levels of MUC1 or TLR-2 expression did not correlate significantly with the levels of cytokines or blood contamination in saliva.
  
  
  CONCLUSIONS
  MUC1 may play a role in the development and/or progression of BMS.
authors: Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Yunkeun-Dong 28, Chongro-Ku, Seoul 110-749, Republic of Korea. hkho@snu.ac.kr
authors: Hong-Seop
authors: HS
authors: Kho
authors: Ji-Youn
authors: JY
authors: Chang
authors: Yoon-Young
authors: YY
authors: Kim
authors: Yongdae
authors: Y
authors: Kim
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000378:metabolism*; D002054:Burning Mouth Syndrome / Q000378:metabolism*; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D017676:Lichen Planus, Oral / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000378:metabolism*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; C434325:MUC-1 monoclonal antibody; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2