title: Detection of Simkania negevensis in cell culture by using a monoclonal antibody.
abstract: In the present study, a monoclonal antibody (mAb), D5-14, raised in our laboratory against Chlamydia trachomatis LGV2 serotype, stained Simkania negevensis inclusions in S. negevensis-infected cells by using the immunofluorescence test. D5-14 mAb, reacting in immunoblot with an approximately 64-66-kDa protein of C. trachomatis LGV2 serotype, recognized a protein with the same molecular mass when tested with S. negevensis elementary bodies.
authors: University of Bologna, Bologna, Italy. manuela.donati@unibo.it
authors: Manuela
authors: M
authors: Donati
authors: Maria
authors: M
authors: Di Paolo
authors: Simone
authors: S
authors: Avanzi
authors: Antonietta
authors: A
authors: Di Francesco
authors: Natascia
authors: N
authors: Fiani
authors: Alison
authors: A
authors: Favaroni
authors: Martina
authors: M
authors: Caracciolo
authors: Roberto
authors: R
authors: Cevenini
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D018929:Cell Culture Techniques / Q000379:methods; D002692:Chlamydia trachomatis / Q000276:immunology*; D002695:Chlamydiales / Q000276:immunology / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal