title: [Subtypes of dengue virus serotypes 2, 3 and 4 isolated in Santander District, Colombia].
abstract: Virus serotypes 2, 3 and 4 that had circulated in Santander District, Colombia in the period 1998-2004 were analyzed. Identifying the subtype of a dengue virus serotype is a useful tool for surveillance of severe risk factors because the strain potential to cause hemorrhagic dengue makes the difference among them. Simultaneous sequence amplification technique known as restriction site specific-polymerase chain reaction (RSS-PCR) was used to determine the subtype by comparing the electrophoretic pattern of the local isolate to the reference virus. Virus serotype 2 corresponded to subtype A similar to the one isolated in Thailand (1996) and to the other isolated in Porto Rico (1986); virus serotypes 3 were of subtype C like the virus found in Sri Lanka (1990), Honduras (1995) and Porto Rico (2000); virus serotypes 4 were a variant of subtype B similar to a virus from Porto Rico (1987) and to another virus from Tahiti (1985). The study confirmed the presence in Colombia of dengue virus subtypes circulating now in the Americas.
authors: Centro de Investigaciones de Enfermedades Tropicales, Universidad Industrial de Santander, Bucaramanga, Colombia. fabiancortesm@yahoo.com
authors: Fabián M
authors: FM
authors: Cortés
authors: Sergio Y
authors: SY
authors: Gómez
authors: Raquel E
authors: RE
authors: Ocazionez
mesh_terms: D000330:Aedes / Q000166:cytology; D000818:Animals; D002478:Cells, Cultured; D003105:Colombia / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D014943:Global Health; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D019595:Severe Dengue / Q000821:virology; D014776:Virus Cultivation
chemical_list: D012367:RNA, Viral