title: Plasmid-based E6-specific siRNA and co-expression of wild-type p53 suppresses the growth of cervical cancer in vitro and in vivo.
abstract: The E6 protein of the oncogenic HPV-16 functions by interfering with the normal cell cycle control mechanisms, particularly those controlled by p53. In this study, we developed a dual expression plasmid that coexpressed-E6-specific siRNA and wild type p53, and to evaluate its effects on cervical cancer growth. We found that simultaneous expression of pSi-E6-P53 caused a robust suppression of tumor growth when compared to the controls either E6-specific siRNA or p53 alone. In conclusion, our findings demonstrate that a combined strategy of co-expressed E6-specific siRNA and p53 synergistically and more effectively suppressed cervical tumor growth when compared with single treatment.
authors: Prostate Diseases Prevention and Treatment Research Center and Department of Pathophysiology, Norman Bethune Medical School, Jilin University, Changchun 130021, PR China.
authors: Xin
authors: X
authors: Li
authors: Yang
authors: Y
authors: Li
authors: Jiadi
authors: J
authors: Hu
authors: Bo
authors: B
authors: Wang
authors: Lijing
authors: L
authors: Zhao
authors: Kun
authors: K
authors: Ji
authors: Baofeng
authors: B
authors: Guo
authors: Di
authors: D
authors: Yin
authors: Yanwei
authors: Y
authors: Du
authors: Dennis J
authors: DJ
authors: Kopecko
authors: Dhananjaya V
authors: DV
authors: Kalvakolanu
authors: Xuejian
authors: X
authors: Zhao
authors: Deqi
authors: D
authors: Xu
authors: Ling
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics; D014169:Transformation, Bacterial; D047368:Tumor Burden; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D051017:Apoptosis Regulatory Proteins; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53