title: Conjugation of R-Phycoerythrin to a Polyclonal Antibody and F (ab')2 Fragment of a Polyclonal Antibody by Two Different Methods.
abstract: R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. Conjugation of antibodies to R-PE facilitates multiple fluorescent staining methods. In the present study polyclonal antibodies and polyclonal F(ab')2 fragment antibodies were conjugated to R-PE by two different methods. The efficiency of the methods was evaluated using Immunocytochemistry (ICC) and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). In the first conjugation method, PE was attached to SMCC linker followed by conjugation of antibody to PE-SMCC. In the second method, SH groups were added onto R-PE molecule, while the antibody was attached to SPDP linker. Then, the antibody-SPDP molecule was conjugated to R-PE. Our results showed that the two conjugation methods did not have any abrogative effects on the antibody binding activity.
authors: Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors: Jafar
authors: J
authors: Mahmoudian
authors: Mahmood
authors: M
authors: Jeddi-Tehrani
authors: Hodjattallah
authors: H
authors: Rabbani
authors: Ahmad Reza
authors: AR
authors: Mahmoudi
authors: Mohammad Mehdi
authors: MM
authors: Akhondi
authors: Amir Hassan
authors: AH
authors: Zarnani
authors: Leila Balaei
authors: LB
authors: Goli
authors: Mahdokht
authors: M
authors: Babaei
authors: Roya
authors: R
authors: Ghods
keywords: Antibody; Conjugation; Immunocytochemistry; Phycoerythrin