title: [Anti-heat shock protein 70 (anti - Hsp 70) antibodies in alcohol use disorder patients].
abstract: The expression of the most important chaperone protein - Hsp70 and autoimmunity directed against it is a risk factor of cardiovascular diseases, increased in subjects with alcohol use disorder (AUD). The aim of the study was to evaluate the level of anti-Hsp 70 protein antibodies (anti-Hsp 70) in sera of AUD patients during abstinence period. Material and methods. The study included 54 subjects with AUD diagnosed basing on DSM IV criteria. In the studied group clinimetric evaluation was performed, plasma lipids, basic transketolase activity in erythrocytes (TK), thiamine pyrophosphate (TPP) activation of transketolase and the level of anti-Hsp 70 antibodies were evaluated as well. Results. In AUD subjects anti-Hsp 70 level was decreased during abstinence period. During first month of abstinency it correlated negatively with total cholesterol concentration (rS=-0.8857, p=0.0188) and the percentage of TPP stimulation (rS=-0.5960, p<0.05), and during 6 months of abstinence with HDL cholesterol (rS=-0.6848, p=0.0289). After 1 year of abstinence anti-Hsp 70 correlated positively with basic TK activity (rS=0.9550, p=0.0008). Sex is an independent factor influencing anti-Hsp 70 level in AUD subjects (B=60.9469, p=0.0435). In multiple regression model including results of clinimetric evaluation and its effect on the level of anti-Hsp 70 antibodies in AUD patients during 1 month of abstinency anti-Hsp 70 correlated with TWEAK scale score (BETA=-1.4543, p=0.0144) and AUDIT score (BETA-=1.2255, p=0.0224). In 2-6 months of abstinency anti-Hsp 70 correlated with TWEAK score (BETA=1.1110, p=0.0418). After 1 year of abstinency anti-Hsp 70 correlated with AUDIT score (BETA=-1.2161, p=0.0210). Conclusion. The autoimmune reaction against Hsp 70 is decreased during abstinency in AUD patients. Its relation with plasma lipids and thiamine deficiency may lead to increased risk of cardiovascular disorders. TWEAK and AUDIT scoring seem to be most useful for clinimetric evaluation in the context of the role of anti-Hsp 70 antibodies.
authors: Zakład Neurochemii i Neuropatologii Katedra Neurologii, Uniwersytet Medyczny im. K. Marcinkowskiego w Poznaniu. slamic@yahoo.com
authors: Sławomir
authors: S
authors: Michalak
authors: Tomasz
authors: T
authors: Piorunek
authors: Danuta
authors: D
authors: Lenart-Jankowska
authors: Krystyna
authors: K
authors: Osztynowicz
authors: Wojciech
authors: W
authors: Kozubski
mesh_terms: D001323:Autoantibodies / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D002310:Cardiomyopathy, Alcoholic / Q000201:enzymology* / Q000276:immunology*; D002784:Cholesterol / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013695:Temperance; D013835:Thiamine Pyrophosphate / Q000097:blood; D014174:Transketolase / Q000097:blood
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D002784:Cholesterol; D014174:Transketolase; D013835:Thiamine Pyrophosphate