title: Evaluation of FT-NIR and ATR-FTIR spectroscopy techniques for determination of minor odd- and branched-chain saturated and trans unsaturated milk fatty acids.
abstract: Determination of nutritionally important trans MUFA, CLA, and OBCFA milk fatty acids (often present in amounts lower than 1.0 g/100 g of total fat) using fast and nondestructive analytical methods would enhance their use as diagnostic tools in dairy herd and human health management. Here, PLS regression using ATR/FTIR spectra indicated potential for determination of trans-11 C18:1 and trans-12 C18:1 (Rcv² ≥ 0.80), and trans-9 C18:1 in very minor concentration (Rcv² > 0.82), as well as anteiso C15:0 (Rcv² = 0.57) and iso C17:0 (Rcv² = 0.61). Furthermore, the main cis-9,trans-11 CLA isomer was predicted well despite the high trans MUFA concentration. Differentiation between the CLA and the trans MUFA signals was evident (based on specific cis/trans bands), and branched-chain saturated fatty acid methyl esters revealed specific iso and anteiso ATR/FTIR absorbance bands. None of the minor FA PLS results with FT-NIR showed interesting potential, except satisfactory predictions for trans-9 C18:1 and cis-9,trans-11 CLA. Overall, ATR/FTIR resulted in better calibrations and provided more specific information for determination of minor milk fatty acids.
authors: Laboratory for Animal Nutrition and Animal Product Quality (LANUPRO), Faculty of Bioscience Engineering, Ghent University, Melle, Belgium.
authors: Ivan
authors: I
authors: Stefanov
authors: Vincent
authors: V
authors: Baeten
authors: Ouissam
authors: O
authors: Abbas
authors: Bruno
authors: B
authors: Vlaeminck
authors: Bernard
authors: B
authors: De Baets
authors: Veerle
authors: V
authors: Fievez
mesh_terms: D000818:Animals; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000032:analysis* / Q000737:chemistry; D005513:Food Inspection / Q000379:methods*; D005583:Fourier Analysis; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000032:analysis / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D019265:Spectroscopy, Near-Infrared; D044242:Trans Fatty Acids / Q000032:analysis* / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D044243:Linoleic Acids, Conjugated; D044242:Trans Fatty Acids