title: Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo.
abstract: In this study the protective effect of phlorotannins, including phloroglucinol, eckol, dieckol, eckstolonol and triphloroethol A, isolated from brown algae Ecklonia cava was investigated against AAPH-induced oxidative stress toxicity in zebrafish embryos. Zebrafish embryos were exposed to AAPH and compared with other groups that were co-exposed with phlorotannins until 2-days post-fertilisation. All phlorotannins scavenged intracellular ROS and prevented lipid peroxidation and reduced AAPH-induced cell death in zebrafish embryos. Negative changes in morphological phenomena, such as pericardial oedema, yolk sac oedema, and growth retardation in zebrafish embryos exposed to AAPH were not observed in groups exposed to phlorotannins. These results clearly indicate that phlorotannins possess prominent antioxidant activity against AAPH-mediated toxicity and might be potential therapeutic agents for treating or preventing several diseases implicated with oxidative stress. This study provides a useful tool for examining the protective effect of antioxidants against AAPH-induced oxidative stress in an alternative in vivo model.
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Min-Cheol
authors: MC
authors: Kang
authors: Seon Heui
authors: SH
authors: Cha
authors: W A J P
authors: WA
authors: Wijesinghe
authors: Sung-Myung
authors: SM
authors: Kang
authors: Seung-Hong
authors: SH
authors: Lee
authors: Eun-A
authors: EA
authors: Kim
authors: Choon Bok
authors: CB
authors: Song
authors: You-Jin
authors: YJ
authors: Jeon
mesh_terms: D000578:Amidines / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D004147:Dioxins / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D000459:Phaeophyceae / Q000737:chemistry*; D010696:Phloroglucinol / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*
chemical_list: D000578:Amidines; D000975:Antioxidants; D001572:Benzofurans; D004147:Dioxins; D020011:Protective Agents; C503840:dieckol; C046728:2,2'-azobis(2-amidinopropane); C060311:eckol; D010696:Phloroglucinol