title: Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1.
abstract: The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4-DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the (1)H, (15)N and (13)C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins.
authors: School of Molecular Bioscience, University of Sydney, Sydney, NSW, 2006, Australia.
authors: Soumya
authors: S
authors: Joseph
authors: Ann H Y
authors: AH
authors: Kwan
authors: Joel P
authors: JP
authors: Mackay
authors: Liza
authors: L
authors: Cubeddu
authors: Jacqueline M
authors: JM
authors: Matthews
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins; D006801:Humans; D060588:LIM Domain Proteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; C548809:Deaf1 protein, mouse; D060588:LIM Domain Proteins; C491976:Lmo4 protein, mouse; D014157:Transcription Factors