title: Novel linear and step-gradient counter-current chromatography for bio-guided isolation and purification of cytotoxic podophyllotoxins from Dysosma versipellis (Hance).
abstract: Dysosma versipellis (Hance) is a famous traditional Chinese medicine for the treatment of snakebite, weakness, condyloma accuminata, lymphadenopathy, and tumors for thousands of years. In this work, four podophyllotoxin-like lignans including 4'-demethylpodophyllotoxin (1), α-peltatin (2), podophyllotoxin (3), β-peltatin (4) as major cytotoxic principles of D. versipellis were successfully isolated and purified by several novel linear and step gradient counter-current chromatography methods using the systems of hexane/ethyl acetate/methanol/water (4:6:3:7 and 4:6:4:6, v/v/v/v). Compared with isocratic elution, linear and step-gradient elution can provide better resolution and save more time for the separation of photophyllotoxin and its congeners. Their cytotoxicities were further evaluated and their structures were validated by high-resolution electrospray TOF MS and nuclear magnetic resonance spectra. All components showed potent anticancer activity against human hepatoma cells HepG2.
authors: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou, China.
authors: Zhi
authors: Z
authors: Yang
authors: Xiaoman
authors: X
authors: Liu
authors: Kuiwu
authors: K
authors: Wang
authors: Xiaoji
authors: X
authors: Cao
authors: Shihua
authors: S
authors: Wu
mesh_terms: D028141:Berberidaceae / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D011034:Podophyllotoxin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014118:Toxins, Biological / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D014118:Toxins, Biological; D011034:Podophyllotoxin