title: Gene expression of tumor necrosis factor receptor associated-factor (TRAF)-1 and TRAF-2 in inflammatory bowel disease.
abstract: OBJECTIVE
  This study aimed to investigate the expression of tumor necrosis factor receptor-associated factor (TRAF)-1 and TRAF-2 in patients with inflammatory bowel disease (IBD).
  
  
  METHODS
  Immunostaining, western blot and real-time polymerase chain reaction (PCR) were used to detect the expression of TRAF-1 and TRAF-2 in colonic mucosa of IBD patients and control. Furthermore, serum protein levels of TRAF-1 and TRAF-2 were measured by ELISA and the receiver operating characteristic (ROC) curve was used to determine their diagnostic value.
  
  
  RESULTS
  The expression of TRAF-1 and TRAF-2 was significantly higher in inflamed and non-inflamed tissues of IBD patients than those in control (P < 0.05). Moreover, inflamed tissues had higher TRAF-1 and TRAF-2 expression than non-inflamed tissues (P < 0.05). Both TRAF-1 and TRAF-2 were shown to have a fair to excellent value in the differentiation of control and IBD patients with the area under the ROC curve (AUROC) of 0.680-1.000 (P < 0.001).
  
  
  CONCLUSIONS
  The activation of TRAF-1 and TRAF-2 may be early events in the pathogenesis of IBD and their functions are not quite the same.
authors: Division of Gastroenterology and Hepatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Disease, Key Laboratory of Gastroenterology & Hepatology, Ministry of Health-Shanghai Jiao Tong University, Shanghai, China.
authors: Yu Qi
authors: YQ
authors: Qiao
authors: Jun
authors: J
authors: Shen
authors: Yan
authors: Y
authors: Gu
authors: Jin Lu
authors: JL
authors: Tong
authors: Xi Tao
authors: XT
authors: Xu
authors: Mei Lan
authors: ML
authors: Huang
authors: Zhi Hua
authors: ZH
authors: Ran
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism; D003424:Crohn Disease / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D047990:TNF Receptor-Associated Factor 1 / Q000096:biosynthesis* / Q000235:genetics; D047992:TNF Receptor-Associated Factor 2 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2