title: Determination of diosbulbin B in rat plasma and urine by LC-MS/MS and its application in pharmacokinetic and urinary excretion studies.
abstract: A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for determination of diosbulbin B in rat plasma and urine after oral administration. The detector was a Q-trap™ mass spectrometer with an electro-spray ionization interface operating in the multiple reaction monitoring mode. After extracted with methyl tert-butyl ether, diosbulbin B and busprione (internal standard, IS) were separated on an Agilent Zorbax C18 column (4.6mm×50mm, 3.5μm) using a gradient mobile phase consisting of water and methanol. Linearity was obtained over the concentration range of 5-5000ng/ml for diosbulbin B in rat plasma and urine. The lower limit of quantitation was 5.0ng/ml. The accuracy (relative error, RE) and precision (relative standard deviation, RSD) of disobulblin B in two biological matrices ranged from -8.2% to 1.4% RE and 1.9 to 10.1% RSD, respectively. The fully validated method was applied to a pharmacokinetic and urine excretion study for the first time. The main pharmacokinetic parameters Tmax, Cmax, T1/2, and Ke were 1.88±0.22h, 18.0±3.1ng/ml, 6.89±1.0h and 0.103±0.01l/h, respectively. A cumulative excretion of disobulbin B in rat urine was 2.69±0.43μg at 60h after dosing, accounting for 0.89% of the total dose.
authors: School of Life Science, Beijing Institute of Technology, Beijing 100081, China. lylyj2001@yahoo.com.cn
authors: Yujuan
authors: Y
authors: Li
authors: Yuan
authors: Y
authors: Tian
authors: Lin
authors: L
authors: Gan
authors: Yongzhi
authors: Y
authors: Li
authors: Bo
authors: B
authors: Chen
authors: Jinyuan
authors: J
authors: Qiao
mesh_terms: D000818:Animals; D002065:Buspirone / Q000097:blood / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C569454:diosbulbin B; D002065:Buspirone