title: Prognostic relationship of metabolic profile obtained of melanoma B16F10.
abstract: Melanoma is a type of cancer that reaches more people in the world, characterized by genetic mutations that trigger the growth of disorganized cells. The diagnosis of skin tumors by invasive techniques has become a risk to the patients, so the search for new non-invasive techniques has been the subject of research in recent years. The objective of this work is to propose a non-invasive method prognosis based on the identification of specific biomarkers of the cancer, known as metabolomics analysis. For this study, we used B16F10 melanoma tumor cells and metabolic profiles were obtained at three time-periods by (1)HNMR and comparison with the cell cycle, apoptosis pathways and proliferation index. The metabolic profiles show the relationship between the metabolites found with energy metabolism, pathways of apoptosis and proliferation, which showed increases in proportion during growth and progression. Were found 29 metabolites, of which the differentially expressed are: lactate, aspartate, glycerol, lipids, alanine, myo-inositol, phosphocholine, choline, acetate, creatine and taurine. Choline and creatine are closely related with tumor progression, and are inversely expressed in later stages of tumor growth, which demonstrates the ability to be markers of tumor progression or monitoring the pharmacological efficacy when combined with other therapies. We conclude that the metabolome appeared as effective non-invasive technique predicts, besides providing possible biomarkers of melanoma.
authors: Biochemistry and Biophysics Laboratory, Butantan Institute, São Paulo, Brazil.
authors: Thiago Antonio
authors: TA
authors: Fedele
authors: Alvaro Carlos
authors: AC
authors: Galdos-Riveros
authors: Homero
authors: H
authors: Jose de Farias e Melo
authors: Alviclér
authors: A
authors: Magalhães
authors: Durvanei Augusto
authors: DA
authors: Maria
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002794:Choline / Q000235:genetics / Q000378:metabolism; D003401:Creatine / Q000235:genetics / Q000378:metabolism; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D018450:Disease Progression; D004734:Energy Metabolism; D005260:Female; D008546:Melanoma, Experimental / Q000235:genetics* / Q000378:metabolism*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis / Q000235:genetics / Q000378:metabolism; D011379:Prognosis; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C530105:Ccnd1 protein, mouse; D019938:Cyclin D1; C505409:Casp3 protein, mouse; D053148:Caspase 3; D003401:Creatine; D002794:Choline