title: Long-lasting airway inflammation associated with equid herpesvirus-2 in experimentally challenged horses.
abstract: The aim of this trial was to investigate the putative involvement of equid herpesvirus 2 (EHV-2) in airway inflammation of adult horses. Six horses received corticosteroid treatment, before either mock infection (n=2) or EHV-2 strain LK4 inoculation (n=4). These four horses were also submitted to immunosuppression 84 days post inoculation. EHV-2 was detected by quantitative PCR in respiratory samples up to respectively 21 days and 14 days. Nested PCR, cloning and sequencing allowed the detection of five different 'field' strains throughout the trial. Neutrophils proportions were transiently increased in respiratory fluids; neutrophilia being significantly associated with concomitant EHV-2 detection. The laboratory findings reproduced in this trial were compatible with sub-clinical lower airway inflammation and suggest that EHV-2 infection should be suspected when investigating poorly-performing horses.
authors: Frank Duncombe Laboratory, 14053 Caen Cedex 4, France; University of Caen Basse-Normandie, SF 4206, ICORE, EA 4655 U2RM, 14032 Caen, France. guillaume.fortier@calvados.fr
authors: Guillaume
authors: G
authors: Fortier
authors: Eric
authors: E
authors: Richard
authors: Erika
authors: E
authors: Hue
authors: Christine
authors: C
authors: Fortier
authors: Stéphane
authors: S
authors: Pronost
authors: Didier
authors: D
authors: Pottier
authors: Laurent
authors: L
authors: Lemaitre
authors: Pierre
authors: P
authors: Lekeux
authors: Kerstin
authors: K
authors: Borchers
authors: Etienne
authors: E
authors: Thiry
mesh_terms: D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000821:virology; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases / Q000473:pathology / Q000821:virology*; D006736:Horses; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007249:Inflammation / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012141:Respiratory Tract Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D018144:Rhadinovirus; D014412:Tumor Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology
chemical_list: D000305:Adrenal Cortex Hormones; D007166:Immunosuppressive Agents
keywords: Equid herpesvirus 2; Horse; Inflammatory airway disease; Tracheal inflammation