title: Protein-directed immobilization of phosphocholine ligands on a gold surface for multivalent C-reactive protein binding.
abstract: The preparation of a synthetic receptor for multivalent protein binding by a directed immobilization of bifunctional ligands was demonstrated using pentameric C-reactive protein (CRP) and a thiolated phosphocholine-containing ligand on a gold surface. CRP consisting of five identical, noncovalently linked subunits and having five phosphocholine-binding sites on the same face was complexed with 12-mercaptododecylphosphocholine. The complexes were reacted with a gold surface, which was blocked with BSA or 2-mercaptoethanol to avoid non-specific binding. CRP binding to the molecularly imprinted monolayer was investigated by surface plasmon resonance, exhibiting high sensitivity with a detection limit as low as 1 pM (0.12 ng/mL) and binding affinity (K(A) ~ 10(-7)-10(-9) M(-1)) comparable to that of immobilized anti- CRP.
authors: Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology, Daegu 711-873, Korea.
authors: Eunjoo
authors: E
authors: Kim
authors: Se Geun
authors: SG
authors: Lee
authors: Hyun-Chul
authors: HC
authors: Kim
authors: Sung Jun
authors: SJ
authors: Lee
authors: Chul Su
authors: CS
authors: Baek
authors: Sang Won
authors: SW
authors: Jeong
mesh_terms: D001665:Binding Sites; D002097:C-Reactive Protein / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008024:Ligands; D010767:Phosphorylcholine; D006046:Gold; D002097:C-Reactive Protein