title: Cryopreservation of osteoblasts by use of a programmed freezer with a magnetic field.
abstract: In order to determine a suitable condition for osteoblasts cryopreservation, murine osteoblasts were freezed by programmed freezer with a magnetic field (CAS freezer). After 7 days cryopreservation at -150°, the number of survival cells immediately after thawing and the growth rate of cultured cells for 48 hours were examined. Gene and protein expression of alkaline phosphatase (ALP), osteopontin (OPN) and bone sialoprotein (BSP) were compared between cryopreserved and non-cryopreserved groups. As a result, a plunging temperature of -30°, a hold-time at -5° for 15 minutes and a 0.1 mT of magnetic field led to the largest survival and growth rate. Moreover, there was no significant difference in ALP, OPN and BSP mRNA and protein expression between cryopreserved and control groups. From these results, it was suggested that the CAS freezer is available for osteoblast cryopreservation and bone tissue banking can be established in the future.
authors: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors: H
authors: H
authors: Koseki
authors: M
authors: M
authors: Kaku
authors: T
authors: T
authors: Kawata
authors: S
authors: S
authors: Kojima
authors: H
authors: H
authors: Sumi
authors: H
authors: H
authors: Shikata
authors: M
authors: M
authors: Motokawa
authors: T
authors: T
authors: Fujita
authors: J
authors: J
authors: Ohtani
authors: K
authors: K
authors: Tanne
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D060526:Magnetic Fields; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D012886:Skull / Q000166:cytology
chemical_list: D058574:Integrin-Binding Sialoprotein; D053495:Osteopontin; D000469:Alkaline Phosphatase