title: Extracellular acidosis modulates the endocytosis and maturation of macrophages.
abstract: Extracellular acidosis is involved in various pathological situations of central nervous system and the effects are largely mediated by acid sensing ion channels (ASICs). However, it remains unclear whether extracellular acidosis affects immune cells. Macrophages are immune cells that play important role in immune reactions. In this study we investigated the impact of extracellular acidosis on the function of bone marrow derived macrophages (BMMs). The results showed that extracellular acidosis upregulated the endocytosis, surface molecular expression and interleukin-10 secretion of BMMs, in which the expression of ASIC1 and ASIC3 was detected. Notably, extracellular acidosis stimulated endocytosis and upregulation of surface molecules expression in BMMs could be abolished by amiloride, a blocker of ASICs, and nonsteroid anti-inflammatory drugs. Our findings provide new insight into the role of extracellular acidosis in the regulation of immune function and suggest ASICs as new targets for the modulation of immune response.
authors: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
authors: Xiaoling
authors: X
authors: Kong
authors: Xianying
authors: X
authors: Tang
authors: Wenjiao
authors: W
authors: Du
authors: Jing
authors: J
authors: Tong
authors: Yutao
authors: Y
authors: Yan
authors: Fang
authors: F
authors: Zheng
authors: Min
authors: M
authors: Fang
authors: Feili
authors: F
authors: Gong
authors: Zheng
authors: Z
authors: Tan
mesh_terms: D062646:Acid Sensing Ion Channel Blockers / Q000494:pharmacology; D062565:Acid Sensing Ion Channels / Q000187:drug effects / Q000378:metabolism; D000138:Acidosis / Q000378:metabolism*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002478:Cells, Cultured; D004705:Endocytosis / Q000276:immunology*; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: C568266:ASIC1 protein, human; C112231:ASIC3 protein, human; D062646:Acid Sensing Ion Channel Blockers; D062565:Acid Sensing Ion Channels; D016753:Interleukin-10; D000584:Amiloride