title: Comparison of autogenic and allogenic bone marrow derived mesenchymal stem cells for repair of segmental bone defects in rabbits.
abstract: Autogenic and allogenic bone marrow derived mesenchymal stem cells (BM-MSCs) were compared for repair of bone gap defect in rabbits. BM-MSCs were isolated from bone marrow aspirates and cultured in vitro for allogenic and autogenic transplantation. A 5mm segmental defect was created in mid-diaphysis of the radius bone. The defect was filled with hydroxyapatite alone, hydroxyapatite with autogeneic BM-MSCs and hydroxyapatite with allogenic BM-MSCs in groups A, B and C, respectively. On an average 3.45×10(6) cells were implanted at each defect site. Complete bridging of bone gap with newly formed bone was faster in both treatment groups as compared to control group. Histologically, increased osteogenesis, early and better reorganization of cancellous bone and more bone marrow formation were discernible in treatment groups as compared to control group. It was concluded that in vitro culture expanded allogenic and autogenic BM-MSCs induce similar, but faster and better healing as compared to control.
authors: Division of Surgery, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors: Rahul Kumar
authors: RK
authors: Udehiya
authors: Amarpal
authors: H P
authors: HP
authors: Aithal
authors: P
authors: P
authors: Kinjavdekar
authors: A M
authors: AM
authors: Pawde
authors: Rajendra
authors: R
authors: Singh
authors: G
authors: G
authors: Taru Sharma
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D016026:Bone Marrow Transplantation / Q000379:methods / Q000662:veterinary*; D001842:Bone and Bones / Q000601:surgery*; D018483:Diaphyses / Q000601:surgery; D017886:Durapatite / Q000627:therapeutic use; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods / Q000662:veterinary*; D010027:Osteotomy / Q000379:methods / Q000662:veterinary; D011817:Rabbits; D011884:Radius / Q000601:surgery; D014182:Transplantation, Autologous / Q000379:methods / Q000662:veterinary; D014184:Transplantation, Homologous / Q000379:methods / Q000662:veterinary
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite