title: Spontaneous ultra fast synthesis of gold nanoparticles using Punica granatum for cancer targeted drug delivery.
abstract: Rapid synthesis of mono-dispersed gold nanoparticles through economically feasible green chemistry approach is highly desirable. In this study, we have developed a method to synthesize mono-dispersed gold nanoparticles (PAuNPs) by mixing gold solution with fruit peel extract of Punica granutum without using any surfactant or external energy. In this method, physiologically stable, biocompatible PAuNPs were formed within 60s. Casein, being a biocompatible polymer, is used to couple the prepared PAuNPs for functionalization of folic acid, which is highly expressed in cancer cells. These functionalized PAuNPs could be used for targeted drug delivery for cancer with enhanced therapeutic efficacy and minimal side effects. PAuNPs were characterized by UV, IR, TEM, Particle size analyzer and zeta potential measurement. In vitro stability of the PAuNPs was also analyzed. Hemocompatibility of PAuNPs was evaluated in human blood samples and found that the particles were hemocompatible. The toxicity of the PAuNPs, 5-Fu and 5Fu@PAuNPs was analyzed in zebrafish embryos. The in vitro cytotoxicity of free 5-Fu, 5Fu@PAuNPs-Fa was investigated against MCF-7 cells (breast cancer) and observed that the amount of 5-Fu required to achieve 50% of growth of inhibition (Ic50) was much lower when compared to free 5-Fu.
authors: Department of Biochemistry, Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai 600020, India.
authors: Moorthy
authors: M
authors: Ganeshkumar
authors: Muniram
authors: M
authors: Sathishkumar
authors: Thangavel
authors: T
authors: Ponrasu
authors: Murugan Girija
authors: MG
authors: Dinesh
authors: Lonchin
authors: L
authors: Suguna
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D001672:Biocompatible Materials; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000473:pathology*; D053938:DNA Fragmentation; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D029561:Lythraceae / Q000737:chemistry*; D061986:MCF-7 Cells; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000008:administration & dosage*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001672:Biocompatible Materials; D010936:Plant Extracts; D006046:Gold