title: [Influence of pre-analytical storage conditions on four plasma coagulation molecular markers measured using a STACIA automatic coagulation analyzer].
abstract: We investigated the effects of specimen storage conditions on the analysis of the coagulation molecular markers, soluble fibrin (SF), thrombin-antithrombin complex (TAT), thrombomodulin (TM) and tissue plasminogen activator inhibitor-1 complex (total PAI-1: tPAI). Marker levels were measured using a STACIA automatic coagulation analyzer. Among these four markers in blood from healthy subjects, only tPAI increased gradually with time, and the differences were especially marked when blood samples were stored at room temperature. Patient blood samples were stored for 4 hours under three different conditions: whole blood storage on ice, storage on ice after centrifugation, and refrigerated storage after centrifugation. Analytical results were compared between the three sets of samples. There were no significant differences in TAT or TM after 4 hours' storage under the different conditions. However, SF was decreased in several samples. In 11 of 14 samples with >20 microg/ml SF, SF levels were reduced by >10 microg/ml when whole blood without centrifugation was stored on ice. tPAI levels increased slightly after storage for 4 hours under all three conditions. These results suggest that centrifugation followed by refrigeration is the optimal storage method for blood samples when all four markers are to be measured simultaneously in the same sample.
authors: Department of Clinical Laboratory, Fukuoka University Hospital, Fukuoka 814-0180, Japan. yaemasataka@fukuoka-u.ac.jp
authors: Masataka
authors: M
authors: Yae
authors: Machiko
authors: M
authors: Sueishi
authors: Yoko
authors: Y
authors: Mikami
authors: Misae
authors: M
authors: Kinoshita
authors: Sumiko
authors: S
authors: Hirano
authors: Kiyoko
authors: K
authors: Nakamura
authors: Yuko
authors: Y
authors: Futata
authors: Hironobu
authors: H
authors: Kawashima
authors: Kumiko
authors: K
authors: Ohkubo
authors: Hiroyasu
authors: H
authors: Ishikura
authors: Akira
authors: A
authors: Matsunaga
mesh_terms: D000990:Antithrombin III; D057205:Automation, Laboratory / Q000295:instrumentation*; D015415:Biomarkers / Q000097:blood; D001780:Blood Coagulation Tests / Q000295:instrumentation*; D005337:Fibrin / Q000032:analysis; D006801:Humans; D010447:Peptide Hydrolases / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D018180:Thrombomodulin / Q000097:blood; D013997:Time Factors
chemical_list: D015415:Biomarkers; D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human; D018180:Thrombomodulin; C046193:antithrombin III-protease complex; D000990:Antithrombin III; D005337:Fibrin; D010447:Peptide Hydrolases