title: Effect of the steroid K-canrenoate on hsp70 expression and tissue damage in transgenic Drosophila melanogaster (hsp70-lacZ) Bg9.
abstract: In the present study the effect of 0.1, 0.2, 0.4, 0.8, and 1.0 µL/mL of the steroid K-canrenoate was evaluated in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9) for 6, 24, and 48 hours of duration. The treatment of 0.1, 0.2, and 0.4 µL/mL of K-canrenoate did not induce the activity of hsp70 significantly compared to the control. The treatments of 0.8 and 1.0 µL/mL of K-canrenoate not only caused tissue damage but also induced a significant increase in the expression of hsp70 for the different durations of exposure. The results of the present study suggest that the K-canrenoate at 0.8 and 1.0 µL/mL is cytotoxic and caused tissue damage in the third instar larvae of transgenic D. melanogaster (hsp70-lacZ) Bg(9).
authors: Drosophila Transgenics Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, U.P., 202002, India. yasir_hasansiddique@rediffmail.com
authors: Yasir Hasan
authors: YH
authors: Siddique
authors: Gulshan
authors: G
authors: Ara
authors: Mohammad
authors: M
authors: Afzal
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D002191:Canrenoic Acid / Q000494:pharmacology*; D004396:Coloring Agents / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology*; D013054:Spectrophotometry, Atomic; D013312:Stress, Physiological; D013997:Time Factors; D014018:Tissue Distribution; D014343:Trypan Blue / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004396:Coloring Agents; D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D000451:Mineralocorticoid Receptor Antagonists; D002191:Canrenoic Acid; D001616:beta-Galactosidase; D014343:Trypan Blue