title: Inhibitory activity of Filipendula ulmaria constituents on recombinant human histidine decarboxylase.
abstract: Histidine decarboxylase (HDC) catalyses the formation of histamine, a bioactive amine. Agents that control HDC activity are beneficial for treating histamine-mediated symptoms, such as allergies and stomach ulceration. We searched for inhibitors of HDC from the ethyl acetate extract of the petal of Filipendula ulmaria, also called meadowsweet. Rugosin D, rugosin A, rugosin A methyl ester (a novel compound), and tellimagrandin II were the main components; these 4 ellagitannins exhibited a non-competitive type of inhibition, with K(i) values of approximately 0.35-1 μM. These K(i) values are nearly equal to that of histidine methyl ester (K(i)=0.46 μM), an existing substrate analogue inhibitor. Our results show that food products contain potent HDC inhibitors and that these active food constituents might be useful for designing clinically available HDC inhibitors.
authors: School of Human Science and Environment, University of Hyogo, 1-1-12 Shinzaike honmachi, Himeji, Hyogo 670-0092, Japan. nitta@shse.u-hyogo.ac.jp
authors: Yoko
authors: Y
authors: Nitta
authors: Hiroe
authors: H
authors: Kikuzaki
authors: Toshiaki
authors: T
authors: Azuma
authors: Yuan
authors: Y
authors: Ye
authors: Motoyoshi
authors: M
authors: Sakaue
authors: Yoshiki
authors: Y
authors: Higuchi
authors: Hirohumi
authors: H
authors: Komori
authors: Hiroshi
authors: H
authors: Ueno
mesh_terms: D004791:Enzyme Inhibitors / Q000737:chemistry*; D031984:Filipendula / Q000737:chemistry*; D006640:Histidine Decarboxylase / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D006640:Histidine Decarboxylase