title: Behavior of bone marrow-derived cells following in vivo transplantation: differentiation into stromal cells with roles in organ maintenance.
abstract: After injection of green fluorescent protein-positive (GFP(+)) bone marrow (BM) cells into lethally irradiated wild-type mice, the organs of the recipient mice [BM transplantation (BMT) mice] were regenerated; however, irradiation of the cecum or spleen (only) blocked their regeneration with loss of injected BM cells. These results suggest that the donor cells first enter the BM and then migrate to the peripheral organs. The maintenance of epithelial structure and function is controlled by interactions between stromal cells and the epithelia; the organ is stable only if the stroma is functioning normally. In BMT mice, intestinal GFP(+) stromal cells were regenerated fairly rapidly although GFP(+) cells were observed only rarely in the intestinal epithelium even if it passes several weeks or months post BMT, indicating that BM-derived stromal cells play a pivotal role in epithelial renewal and are crucial for maintaining organ structure and function. BM-derived cells in the periphery possess a special key to return to the BM and then to migrate to various organs to become resident cells.
authors: Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, Japan. jm-otaguchi@kochi-u.ac.jp
authors: Osamu
authors: O
authors: Taguchi
authors: Kunio
authors: K
authors: Tsujimura
authors: Keiichi
authors: K
authors: Kontani
authors: Yosuke
authors: Y
authors: Harada
authors: Sachiyo
authors: S
authors: Nomura
authors: Hiroshi
authors: H
authors: Ikeda
authors: Akimichi
authors: A
authors: Morita
authors: Hideshi
authors: H
authors: Sugiura
authors: Norio
authors: N
authors: Hayashi
authors: Yasushi
authors: Y
authors: Yatabe
authors: Masao
authors: M
authors: Seto
authors: Masae
authors: M
authors: Tatematsu
authors: Toshitada
authors: T
authors: Takahashi
authors: Atsuki
authors: A
authors: Fukushima
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D004848:Epithelium / Q000473:pathology / Q000528:radiation effects; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D009928:Organ Specificity / Q000528:radiation effects; D010227:Parabiosis; D012038:Regeneration / Q000502:physiology* / Q000528:radiation effects; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors; D014965:X-Rays
chemical_list: D049452:Green Fluorescent Proteins; D017493:Leukocyte Common Antigens