title: Expression of histone acetylases p300 and PCAF in pediatric astrocytomas.
abstract: OBJECTIVE
  The protein 300 (p300) and p300/CBP-binding protein-associated factor (PCAF) are enzymes with histone acetyltransferase (HAT) activity, a function that can become deregulated in different tumors and affect biological responses.
  
  
  METHODS
  Due to the lack of information on the deregulation of these HATs in pediatric tumors, this study evaluated the expression of both the mRNA and proteins of p300 and PCAF in 54 samples of pediatric astrocytomas embedded in paraffin.
  
  
  RESULTS
  PCAF was not expressed in normal brain tissue. In grade I tumors, the expression of p300 (1.1 ± 0.1) and PCAF (1.2 ± 0.11) was greater than those observed in grade III tumors: 0.72 ± 0.15 for p300 and 0.55 ± 0.11 for PCAF, and grade IV tumors: 0.74 ± 0.13 for p300 and 0.55 ± 0.13 for PCAF (p < 0.05). Immunohistochemical staining revealed the same tendency towards a decrease in the expression of the protein as the degree of clinical severity increased. Patients with recurrent grades I, III, and IV tumors had the highest levels of PCAF, compared to those who showed no recurrence (p < 0.05).
  
  
  CONCLUSIONS
  This work describes and confirms that these HATs play important roles in regulating genes and in the biological behavior of pediatric astrocytomas.
authors: Departamento de Patología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720, Mexico City, Mexico.
authors: Pilar
authors: P
authors: Eguía-Aguilar
authors: Departamento de Patología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720, Mexico City, Mexico.
authors: Mario
authors: M
authors: Solís-Paredes
authors: Departamento de Patología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720, Mexico City, Mexico.
authors: Paulina
authors: P
authors: Reyes-Cid
authors: Departamento de Patología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720, Mexico City, Mexico.
authors: Mario
authors: M
authors: Perezpeña-Diazconti
authors: Departamento de Neurocirugía, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720, Mexico City, Mexico.
authors: Fernando Chico-Ponce
authors: FC
authors: de León
authors: Departamento de Patología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720, Mexico City, Mexico.
authors: Stanislaw
authors: S
authors: Sadowinski-Pine
authors: Departamento de Patología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720, Mexico City, Mexico. rosco26@excite.com.
authors: Francisco
authors: F
authors: Arenas-Huertero
mesh_terms: D001254:Astrocytoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002648:Child; D050881:E1A-Associated p300 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D050880:p300-CBP Transcription Factors / Q000235:genetics* / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
chemical_list: D050881:E1A-Associated p300 Protein; D012333:RNA, Messenger; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C494360:EP300 protein, human
keywords: Chromatin; GBM; HAT; PCAF; Pediatric astrocytoma; Recurrent; p300