title: Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae).
abstract: Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (primer) ranged from 3 to 11 and expected heterozygosities varied from 0.309 to 0.884. The probability of locus identity was ~1.88 x 10(-4) and the probability of paternity exclusion was ~0.9367. The 5 microsatellite primer pairs may be suitable for population genetic studies such as parentage and fine-scale genetic analyses of this species.
authors: Laboratório de Genética & Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil tellesmpc@gmail.com.
authors: M P C
authors: MP
authors: Telles
authors: J B
authors: JB
authors: Silva
authors: L V
authors: LV
authors: Resende
authors: R P
authors: RP
authors: Vianello
authors: L J
authors: LJ
authors: Chaves
authors: T N
authors: TN
authors: Soares
authors: R G
authors: RG
authors: Collevatti
mesh_terms: D000483:Alleles; D018744:DNA, Plant / Q000235:genetics; D005828:Genetics, Population; D015698:Genomic Library; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D027842:Syzygium / Q000235:genetics*
chemical_list: D018744:DNA, Plant