title: Acute kidney injury reduces phagocytic and microbicidal capacities of alveolar macrophages.
abstract: OBJECTIVE
  Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. The present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI.
  
  
  METHODS
  The alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia.
  
  
  RESULTS
  Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. The killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-γ (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI.
  
  
  CONCLUSIONS
  Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA.
authors: Laboratório de Imunologia Clínica e Experimental, Disciplina de Nefrologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors: Reinaldo C
authors: RC
authors: Silva
authors: Maristella A
authors: MA
authors: Landgraf
authors: Matheus
authors: M
authors: Corrêa-Costa
authors: Patricia
authors: P
authors: Semedo
authors: Marcos A
authors: MA
authors: Cenedeze
authors: Alvaro
authors: A
authors: Pacheco-Silva
authors: Richardt G
authors: RG
authors: Landgraf
authors: Niels O S
authors: NO
authors: Câmara
mesh_terms: D058186:Acute Kidney Injury / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000502:physiology; D016676:Macrophages, Alveolar / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D010587:Phagocytosis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D018932:Chemokine CCL2; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma; D010047:Ovalbumin; D051547:Heme Oxygenase-1