title: Production of Pentameric Cholera Toxin B Subunit in Escherichia coli.
abstract: Cholera toxin B subunit (CTB) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. Production of CTB has been carried out in the bacterial, plant, insect and yeast expression systems. In this study the expression of the CTB containing a 6XHis-tagged was performed by Escherichia coli (E.coli) M15. The yield of purified pentameric recombinant CTB was about 1 mg/l. Western blot analysis demonstrated that the recombinant CTB was antigenically active. In addition, GM1-ganglioside ELISA showed that recombinant CTB binds to GM1-gangelioside receptor, confirming disulfide bond formation and proper folding of the recombinant protein in E.coli. Overall, in regard to the vast applications of CTB in medicine, this bacterial expression system will be a fast, cost-effective and simple system for production of pentameric CTB and CTB conjugated proteins.
authors: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Farida
authors: F
authors: Dakterzada
authors: Ashraf Mohabati
authors: AM
authors: Mobarez
authors: Mehryar Habibi
authors: MH
authors: Roudkenar
authors: Mehdi
authors: M
authors: Forouzandeh
keywords: Cholera toxin B Subunit; Enzyme linked immunosorbent assay; Western blotting