title: Morphology and nanomechanics of sensory neurons growth cones following peripheral nerve injury.
abstract: A prior peripheral nerve injury in vivo, promotes a rapid elongated mode of sensory neurons neurite regrowth in vitro. This in vitro model of conditioned axotomy allows analysis of the cellular and molecular mechanisms leading to an improved neurite re-growth. Our differential interference contrast microscopy and immunocytochemistry results show that conditioned axotomy, induced by sciatic nerve injury, did not increase somatic size of adult lumbar sensory neurons from mice dorsal root ganglia sensory neurons but promoted the appearance of larger neurites and growth cones. Using atomic force microscopy on live neurons, we investigated whether membrane mechanical properties of growth cones of axotomized neurons were modified following sciatic nerve injury. Our data revealed that neurons having a regenerative growth were characterized by softer growth cones, compared to control neurons. The increase of the growth cone membrane elasticity suggests a modification in the ratio and the inner framework of the main structural proteins.
authors: Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, Montpellier, France.
authors: Marta
authors: M
authors: Martin
authors: Ouafa
authors: O
authors: Benzina
authors: Vivien
authors: V
authors: Szabo
authors: Attila-Gergely
authors: AG
authors: Végh
authors: Olivier
authors: O
authors: Lucas
authors: Thierry
authors: T
authors: Cloitre
authors: Frédérique
authors: F
authors: Scamps
authors: Csilla
authors: C
authors: Gergely
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D019771:Axotomy / Q000379:methods; D001696:Biomechanical Phenomena; D002478:Cells, Cultured; D004548:Elasticity; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D020439:Growth Cones / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D051379:Mice; D018625:Microscopy, Atomic Force; D008858:Microscopy, Phase-Contrast; D009416:Nerve Regeneration / Q000502:physiology; D059348:Peripheral Nerve Injuries / Q000503:physiopathology*; D012584:Sciatic Nerve / Q000293:injuries; D011984:Sensory Receptor Cells / Q000378:metabolism / Q000502:physiology*; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D014404:Tubulin; C462941:beta3 tubulin, mouse