title: Peptide microarrays for profiling of serine/threonine kinase activity of recombinant kinases and lysates of cells and tissue samples.
abstract: Peptide microarray technology can be used to identify substrates for recombinant kinases, to measure kinase activity and changes thereof in cell lysates and lysates from fresh frozen (tumor) tissue. The effect of kinase inhibitors on the kinase activities in relevant tissues can be investigated as well. The method for performing experiments on dynamic peptide microarrays with real-time readout is described, as well as the influence of assay parameters and suggestions for optimization of experiments.
authors: PamGene International BV, 's-Hertogenbosch, The Netherlands.
authors: Riet
authors: R
authors: Hilhorst
authors: Liesbeth
authors: L
authors: Houkes
authors: Monique
authors: M
authors: Mommersteeg
authors: Joyce
authors: J
authors: Musch
authors: Adriënne
authors: A
authors: van den Berg
authors: Rob
authors: R
authors: Ruijtenbeek
mesh_terms: D000818:Animals; D002457:Cell Extracts; D002458:Cell Fractionation; D057075:Enzyme Assays; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D007700:Kinetics; D061986:MCF-7 Cells; D010446:Peptide Fragments / Q000737:chemistry*; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000379:methods*; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D002457:Cell Extracts; D055767:Immobilized Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D017346:Protein Serine-Threonine Kinases