title: Cyclobacterium caenipelagi sp. nov., isolated from a tidal flat sediment, and emended description of the genus Cyclobacterium.
abstract: A Gram-staining-negative, aerobic, non-flagellated, non-gliding and ring-like or horseshoe-shaped bacterial strain, designated HD-17(T), was isolated from a tidal flat sediment in the Korean peninsula. Strain HD-17(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HD-17(T) fell within the clade comprising species of the genus Cyclobacterium. Strain HD-17(T) exhibited 16S rRNA gene sequence similarity values of 93.8-98.8% to the type strains of species of the genus Cyclobacterium. Strain HD-17(T) contained MK-7 as the predominant menaquinone and iso-C(15:0), summed feature 3 (C(16:1)ω6c and/or C(16:1)ω7c), anteiso-C(15:0) and iso-C(15:1) G as the major fatty acids. The major polar lipids of strain HD-17(T) were phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain HD-17(T) was 43.8 mol% and its DNA-DNA relatedness values with Cyclobacterium amurskyense KCTC 12363(T), Cyclobacterium qasimii KCTC 23011(T) and Cyclobacterium marinum KCTC 2917(T) were 10.4, 7.6 and 5.3%, respectively. The phylogenetic and genetic distinctiveness and several differentiating phenotypic properties revealed that strain HD-17(T) was separate from other species of the genus Cyclobacterium. On the basis of the data presented, strain HD-17(T) represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium caenipelagi sp. nov. is proposed. The type strain is HD-17(T) ( = KCTC 32178(T) =CCUG 63247(T)). An emended description of the genus Cyclobacterium is also provided.
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.|University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea.
authors: Yong-Taek
authors: YT
authors: Jung
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.|University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea.
authors: Jung-Sook
authors: JS
authors: Lee
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7