title: Efficiency of pH-sensitive fusogenic polymer-modified liposomes as a vaccine carrier.
abstract: The usefulness of pH-sensitive fusogenic polymer-(succinylated poly(glycidol)-(SucPG-) modified liposomes as a vaccine carrier in the induction of immune responses was evaluated. Mice were intraperitoneally immunized with ovalbumin- (OVA-) containing SucPG-modified liposomes. After immunization, significant OVA-specific antibodies were detected in the serum. When sera were analyzed for isotype distribution, OVA-specific IgG1 antibody responses were noted in mice immunized with OVA-containing polymer-unmodified liposomes, whereas immunization with OVA-containing SucPG-modified liposomes resulted in the induction of OVA-specific IgG1, IgG2a, and IgG3 Ab responses. In spleen lymphocytes from mice immunized with OVA-containing SucPG-modified liposomes, both IFN-γ-(Th1-type-) and IL-4-(Th2 type-) specific mRNA were detected. Moreover, substantial production of IFN-γ and IL-4 was demonstrated in spleen cells from OVA-containing SucPG-modified liposomes in vitro. These results suggest that the pH-sensitive fusogenic polymer-(SucPG-) modified liposomes would serve effectively as an antigen delivery vehicle for inducing Th1 and Th2 immune responses.
authors: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan. swatarai@vet.osakafu-u.ac.jp
authors: Shinobu
authors: S
authors: Watarai
authors: Tana
authors: T
authors: Iwase
authors: Tomoko
authors: T
authors: Tajima
authors: Eiji
authors: E
authors: Yuba
authors: Kenji
authors: K
authors: Kono
mesh_terms: D000818:Animals; D004337:Drug Carriers; D005260:Female; D006863:Hydrogen-Ion Concentration; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D011409:Propylene Glycols / Q000737:chemistry / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013386:Succinates / Q000737:chemistry / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D004337:Drug Carriers; D007074:Immunoglobulin G; D008081:Liposomes; D011409:Propylene Glycols; D013386:Succinates; D014612:Vaccines; C502907:polyglycidol; D015847:Interleukin-4; D007371:Interferon-gamma; D010047:Ovalbumin