title: Peyer's patch-mediated intestinal immune system modulating activity of pectic-type polysaccharide from peel of Citrus unshiu.
abstract: An intestinal immune system modulating polysaccharide (CUI-3IIb-3-2, 18kDa) was purified from Citrus unshiu peel. CUI-3IIb-3-2 mainly comprised GalA, GlcA, Ara, Gal and Rha, and it consisted of 4-linked GalA, terminal Araf, 4- or 5-linked/3,4- or 3,5-branched Ara, terminal Gal, and 2-linked/2,4-branched Rha. After CUI-3IIb-3-2 digestion by endo-α-d-(1→4)-polygalacturonase, its hydrolysate was fractionated into PG-1 and PG-2. Methylation analyses of PG-1 and PG-2 using base-catalysed β-elimination suggested that CUI-3IIb-3-2 be assumed as pectic-type polysaccharide. Since the activities of PG-1 and PG-2 were potently decreased, the whole polysaccharide structure of CUI-3IIb-3-2 would be essential to maintain the activity. Meanwhile, when CUI-3IIb was orally administered in mice, bone marrow cell proliferation and GM-CSF/IL-6 production from Peyer's patch cell were significantly higher (1.76- and 2.03/2.51-fold, respectively) than a saline. Therefore, a pectic-type polysaccharide from citrus peel could stimulate Peyer's patches and produce hematopoietic growth factors resulted in bone marrow cell proliferation.
authors: Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.
authors: Hyung-Joo
authors: HJ
authors: Suh
authors: Hyun-Seuk
authors: HS
authors: Yang
authors: Kyung-Soo
authors: KS
authors: Ra
authors: Dong-Ouk
authors: DO
authors: Noh
authors: Ki-Han
authors: KH
authors: Kwon
authors: Jong-Hyun
authors: JH
authors: Hwang
authors: Kwang-Won
authors: KW
authors: Yu
mesh_terms: D000818:Animals; D002957:Citrus / Q000737:chemistry*; D005260:Female; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010581:Peyer's Patches / Q000187:drug effects* / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D007155:Immunologic Factors; D010936:Plant Extracts; D010368:Pectins