title: [Inductive effect of zinc oxide nanoparticles on interleukin 8 gene expression in human bronchial epithelial cells and its regulatory mechanism].
abstract: OBJECTIVE
  To clarify the effect of zinc oxide nanoparticles (ZnO-NPs) (30 nm in diameter) on the interleukin 8 (IL-8) gene expression in human bronchial epithelial cells (BEAS-2B) and its regulatory mechanism.
  
  
  METHODS
  BEAS-2B cells were used in the study. The MTT assay was employed to evaluate the damage to BEAS-2B cells by ZnO-NPs. RT-PCR and ELISA were used to measure the mRNA and protein expression levels of IL-8 in the BEAS-2B cells exposed to ZnO-NPs. The IL-8 mRNA decay assay was used to determine the effect of ZnO-NPs on IL-8 mRNA stability.
  
  
  RESULTS
  Exposure to ZnO-NPs significantly increased the level of IL-8 mRNA in BEAS-2B cells and the level of IL-8 protein in supernatant medium. The transcription inhibitor significantly reduced the mRNA expression of IL-8 induced by ZnO-NPs. ZnO-NPs significantly delayed IL-8 mRNA degradation in the BEAS-2B cells that were pretreated with actinomycin D for terminating IL-8 mRNA synthesis.
  
  
  CONCLUSIONS
  ZnO-NPs can increase the mRNA and protein expression levels of IL-8 and IL-8 mRNA stability in BEAS-2B cells.
authors: Department of Ocupational and Environmental health, Collage of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors: Yang
authors: Y
authors: Lu
authors: Lei
authors: L
authors: Xu
authors: Zhen
authors: Z
authors: Yan
authors: Yi-ming
authors: YM
authors: Wu
authors: Wei-dong
authors: WD
authors: Wu
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D053758:Nanoparticles; D020871:RNA Stability / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D015034:Zinc Oxide / Q000009:adverse effects*
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D012333:RNA, Messenger; D015034:Zinc Oxide