title: Effect of bushenyisui formula on brain tissue apoptosis and Bcl-2 in beta-amyloid protein-induced Alzheimer's disease rat models.
abstract: OBJECTIVE
  To investigate the effect of Bushenyisui Formula on cell apoptosis and positive B cell lymphoma (Bcl-2) in the Brain of rat models of Alzheimer's disease (AD) induced by beta-amyloid protein (Abeta) and the mechanism underlying the effect.
  
  
  METHODS
  Total of 40 SD rats, 20 females and 20 males, were randomly assigned to 4 groups, controlled group (A), model group (B), conventional treatment group (C) and Bushenyisui Formula treatment (BYFT) group (D), 10 rats in each group. Abeta 1-42 was injected into the bilateral hippocampus of the rats in group B, C and D to create the models of AD. Sham operation was performed on the rats of group A in the same way by injecting equal volume of 0.9% sodium chloride solution into their bilateral hippocampus. 5 days after operation, Bushenyisui Formula was intraperitoneally administered at a dose of 450 mg/kg to the rats of group D (QD) for 20 days. Equal volume of 0.9% sodium chloride solution was intraperitoneally injected into the rats of group B with the same procedure. C suspension (20 mg/mL) was intraperitoneally injected into the rats of group B with the same procedure. The number of apoptotic cells in Brain and the positive Bcl-2 were counted. The changes of learning and memory abilities were evaluated using Y-maze.
  
  
  RESULTS
  Right after the establishment of the models, group B, C and D compared to group A respectively, the outcomes of Y-maze were significantly different from that of group A, which suggested obvious learning and memory disorder in those groups (P < 0.01). After treatment, the times of electronic shocks of group C and D were significantly less than that of group B (P < 0.05), and the numbers of apoptotic cells and positive Bcl-2 were significantly different from those of group B, apoptotic sells' number of group C and D smaller than that of group B and the number of positive Bcl-2 greater than that of group B.
  
  
  CONCLUSIONS
  Bushenyisui Formula could increase the number of Bcl-2 in brain, which improved the function of nervous system pertaining to learning and memory abilities.
authors: Department of Hospital Office, Third Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450008, China. sanfuyuan@163.com
authors: Shuke
authors: S
authors: Cui
authors: Yan
authors: Y
authors: Sun
authors: Chengzang
authors: C
authors: Liu
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C545487:APP protein, human; D016564:Amyloid beta-Protein Precursor; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2