title: Developing solid particulate vaccine adjuvants: surface bound antigen favours a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity.
abstract: This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.
authors: Medicines Research Unit, School of Life and Health Sciences, Aston University, Birmingham, UK, B4 7ET.
authors: Daniel J
authors: DJ
authors: Kirby
authors: Randip
authors: R
authors: Kaur
authors: Else M
authors: EM
authors: Agger
authors: Peter
authors: P
authors: Andersen
authors: Vincent W
authors: VW
authors: Bramwell
authors: Yvonne
authors: Y
authors: Perrie
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014612:Vaccines / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D000276:Adjuvants, Immunologic; C557979:Ag85B-ESAT-6 fusion antigen; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D016207:Cytokines; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D014612:Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid