title: Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions.
abstract: We demonstrate quantitative multicolor three-dimensional (3D) subdiffraction imaging of the structural arrangement of fluorescent protein fusions in living Caulobacter crescentus bacteria. Given single-molecule localization precisions of 20-40 nm, a flexible locally weighted image registration algorithm is critical to accurately combine the super-resolution data with <10 nm error. Surface-relief dielectric phase masks implement a double-helix response at two wavelengths to distinguish two different fluorescent labels and to quantitatively and precisely localize them relative to each other in 3D.
authors: Department of Chemistry, Stanford University, Stanford, California 94305, United States.
authors: Andreas
authors: A
authors: Gahlmann
authors: Jerod L
authors: JL
authors: Ptacin
authors: Ginni
authors: G
authors: Grover
authors: Sean
authors: S
authors: Quirin
authors: Lexy
authors: L
authors: von Diezmann
authors: Marissa K
authors: MK
authors: Lee
authors: Mikael P
authors: MP
authors: Backlund
authors: Lucy
authors: L
authors: Shapiro
authors: Rafael
authors: R
authors: Piestun
authors: W E
authors: WE
authors: Moerner
mesh_terms: D001426:Bacterial Proteins / Q000648:ultrastructure*; D016935:Caulobacter crescentus / Q000737:chemistry; D003116:Color
chemical_list: D001426:Bacterial Proteins