title: Production, purification and partial characterization of four lipases from a thermophile isolated from Deception Island.
abstract: Four lipases were purified from ID17, a thermophilic bacterium belonging to Geobacillus genus isolated from Deception Island, Antarctica. Lipase activity was detected by opacity test and p-nitrophenyl laurate methods. Lipase production was better in a medium containing tryptone as the carbon and nitrogen source, without non-ionic detergents and pH 7.5. Proteins were ultrafiltered from supernatant and separated using anion exchange and size exclusion chromatography resulting in four distinct fractions with lipase activity (called Lip1-4). Purified lipases showed an optimal pH at 9.0, 9.5, 10.0 and 8.0 and temperature at 65, 70, 75 and 80 °C for Lip1-4, respectively. Lip1 and Lip2 showed higher activity using p-nitrophenol decanoate as substrate, whereas Lip3 and Lip4 prefer p-nitrophenol laurate. Based on their molecular weight Lip1 and Lip2 are trimeric and pentameric proteins, respectively, whereas Lip3 and Lip4 are monomeric proteins. Lip1 was exceptionally thermostable maintaining 70 % of its activity after incubating it at 70 °C for 8 h. Based on their characteristics, the four lipases obtained from ID17 are good candidates to understand the mechanisms of lipase stability and to be used in different types of industrial applications.
authors: Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago, Chile. pmunoz@bioscience.cl
authors: Patricio A
authors: PA
authors: Muñoz
authors: Daniela N
authors: DN
authors: Correa-Llantén
authors: Jenny M
authors: JM
authors: Blamey
mesh_terms: D000864:Antarctic Regions; D056506:Geobacillus / Q000737:chemistry / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007848:Laurates / Q000378:metabolism; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism; D055503:Protein Multimerization; D013379:Substrate Specificity
chemical_list: D007848:Laurates; D009596:Nitrophenols; C050014:4-nitrophenyl laurate; D008049:Lipase; C024836:4-nitrophenol