title: Optical detection of nanoparticle-enhanced human papillomavirus genotyping microarrays.
abstract: In this study, we propose a new detection method of nanoparticle-enhanced human papillomavirus genotyping microarrays using a DVD optical pick-up with a photodiode. The HPV genotyping DNA chip was labeled using Au/Ag core-shell nanoparticles, prepared on a treatment glass substrate. Then, the bio information of the HPV genotyping target DNA was detected by measuring the difference of the optical signals between the DNA spots and the background parts for cervical cancer diagnosis. Moreover the approximate linear relationship between the concentration of the HPV genotyping target DNA and the optical signal depending on the density of Au/Ag core-shell nanoparticles was obtained by performing a spot finding algorithm. It is shown that the nanoparticle-labeled HPV genotyping target DNA can be measured and quantified by collecting the low-cost photodiode signal on the treatment glass chip, replacing high-cost fluorescence microarray scanners using a photomultiplier tube.
authors: Nanostorage Inc., #714 DMC Hi-Tech Industry Center, 1580, Sangam-Dong, Mapo-Ku, Seoul 121-270,  Korea.
authors: Xue Zhe
authors: XZ
authors: Li
authors: Sookyung
authors: S
authors: Kim
authors: Wonhyung
authors: W
authors: Cho
authors: Seung-Yop
authors: SY
authors: Lee
keywords: (170.3880) Medical and biological imaging; (170.3890) Medical optics instrumentation; (170.4580) Optical diagnostics for medicine