title: miR-29b suppresses CML cell proliferation and induces apoptosis via regulation of BCR/ABL1 protein.
abstract: MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally and are critical for many cellular pathways. Recent evidence has shown that aberrant miRNA expression profiles and unique miRNA signaling pathways are present in many cancers. Here, we demonstrate that miR-29b is markedly lower expressed in CML patient samples. Bioinformatics analysis reveals a conserved target site for miR-29b in the 3'-untranslated region (UTR) of ABL1. miR-29b significantly suppresses the activity of a luciferase reporter containing ABL1-3'UTR and this activity is not observed in cells transfected with mutated ABL1-3'UTR. Enforced expression of miR-29b in K562 cells inhibits cell growth and colony formation ability thereby inducing apoptosis through cleavage of procaspase 3 and PARP. Furthermore, K562 cells transfected with a siRNA targeting ABL1 show similar growth and apoptosis phenotypes as cells overexpression of miR-29b. Collectively, our results suggest that miR-29b may function as a tumor suppressor by targeting ABL1 and BCR/ABL1.
authors: Department of Clinical Hematology, Key Laboratory of Laboratory Medical Diagnostics Designated of Ministry of Education, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors: Yajuan
authors: Y
authors: Li
authors: Haixia
authors: H
authors: Wang
authors: Kun
authors: K
authors: Tao
authors: Qing
authors: Q
authors: Xiao
authors: Zhenglan
authors: Z
authors: Huang
authors: Liang
authors: L
authors: Zhong
authors: Weixi
authors: W
authors: Cao
authors: Jianping
authors: J
authors: Wen
authors: Wenli
authors: W
authors: Feng
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D049109:Cell Proliferation / Q000187:drug effects; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D016147:Genes, Tumor Suppressor / Q000502:physiology; D016313:Genes, abl / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051562:Proto-Oncogene Proteins c-bcr / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D016044:Fusion Proteins, bcr-abl; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr