title: [Use of Amplicor HIV-1 DNA test v.1.5 in diagnosis of perinatal infection from HIV-1 in Cuba].
abstract: BACKGROUND
  HIV-1 proviral DNA amplification is the preferential method for HIV diagnosis infection in infants and it has been used in Cuba since 1992. AMPLICOR HIV-1 DNA kit is an in vitro qualitative assay for the detection of HIV-1 proviral DNA in the whole blood.
  
  
  OBJECTIVE
  this paper showed the results of the use of this kit for the first time in Cuba for the perinatal diagnosis of HIV-1 infection.
  
  
  METHODS
  three hundred forty six whole blood samples from children of HIV seropositive women were analyzed by the AMPLICOR HIV-1 DNA kit in the period 2005-2007.
  
  
  RESULTS
  among the tested samples, six were positive, and 340 negative.
  
  
  CONCLUSIONS
  the assay was reproducible under the Cuban conditions and the achieved results made the diagnosis and follow up of children of HIV-1 seropositive mothers possible.
authors: Laboratorio de Investigaciones del SIDA (LISIDA), La Habana, Cuba. lisida@infomed.sld.cu
authors: Liuber Y
authors: LY
authors: Machado Zaldivar
authors: Madeline
authors: M
authors: Blanco de Armas
authors: Ana Luisa
authors: AL
authors: Lubián Caballero
authors: Héctor M
authors: HM
authors: Díaz Torres
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods*; D003462:Cuba; D004279:DNA, Viral / Q000032:analysis*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1; D006801:Humans; D007231:Infant, Newborn
chemical_list: D004279:DNA, Viral