title: The effect of pretreatment with substrates on the activity of immobilized pancreatic lipase.
abstract: Porcine pancreatic lipase was covalently immobilized on polyvinyl alcohol using adipoyl dichloride as a cross-linker. The effect of pre-treatment of lipase previously with various types of oils on immobilization efficiency was investigated. The increment in immobilization efficiency was observed after pre-treatment of lipases with oils. The highest immobilization efficiency obtained was 20% (v/v) for olive oil pre-treated lipase, which was 8 times higher than that of non-pretreated immobilized lipase. Immobilized lipase had better stability and had some advantages in comparison with free enzyme.
authors: Faculty of Science, Biochemistry Department, Ege University , Bornova/Izmir , Turkey.
authors: Guliz
authors: G
authors: Ak
authors: Yeser
authors: Y
authors: Aktuna
authors: Funda
authors: F
authors: Kartal
authors: Ali
authors: A
authors: Kilinc
mesh_terms: D000272:Adipates / Q000737:chemistry; D000818:Animals; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D000069463:Olive Oil; D010179:Pancreas / Q000201:enzymology*; D010938:Plant Oils / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013379:Substrate Specificity; D013552:Swine
chemical_list: D000272:Adipates; D004800:Enzymes, Immobilized; D000069463:Olive Oil; D010938:Plant Oils; C503083:adipoyl chloride; D011142:Polyvinyl Alcohol; D008049:Lipase