title: Intercellular adhesion molecule-1 (ICAM-1, CD54) is increased in adhesive capsulitis.
abstract: BACKGROUND
  The purpose of this study was to investigate the presence of intercellular adhesion molecule-1 (ICAM-1) in shoulders with adhesive capsulitis ("frozen shoulder").
  
  
  METHODS
  Glenohumeral capsular tissue was obtained from twenty-six patients (seventeen with adhesive capsulitis and nine controls), and ICAM-1 was evaluated with use of oligonucleotide arrays, real-time reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemistry. ICAM-1 was also evaluated in synovial fluid with use of western blotting (six patients with adhesive capsulitis and two controls) and in peripheral blood with use of an enzyme-linked immunosorbent assay (ELISA) (thirty-two patients with adhesive capsulitis, twenty with diabetes mellitus, and fourteen controls). The effect of ICAM-1 treatment on gene expression of cytokines related to inflammation and fibrosis was evaluated in cultured normal human synovial cells.
  
  
  RESULTS
  The level of ICAM-1 was significantly greater in capsular tissue from the glenohumeral joint of patients with adhesive capsulitis compared with controls as measured by oligonucleotide array analysis (0.12 ± 0.01 compared with 0.09 ± 0.00 arbitrary units) (p = 0.001), real-time RT-PCR (1.70 ± 0.19 compared with 0.67 ± 0.24 arbitrary units) (p < 0.05), and immunohistochemical staining. ICAM-1 was also significantly increased in the synovial fluid of patients with adhesive capsulitis (1.70 ± 0.18 arbitrary units) compared with normal controls (0.48 ± 0.17) (p < 0.05) and in serum of patients with adhesive capsulitis (633.22 ± 59.14 ng/mL) and patients with diabetes mellitus (671.25 ± 27.08 ng/mL) compared with controls (359.86 ± 44.29 ng/mL) (p < 0.05). Gene expression of cytokines related to inflammation and fibrosis in synoviocytes cultured in vitro was greater after three days of treatment with ICAM-1 and with ICAM-1 with glucose compared with untreated cells.
  
  
  CONCLUSIONS
  ICAM-1 was increased in patients with adhesive capsulitis, similar to the increase that has been reported in patients with diabetes mellitus.
authors: Department of Orthopedic Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, South Korea. kysoos@catholic.ac.kr
authors: Yang-Soo
authors: YS
authors: Kim
authors: Jung-Man
authors: JM
authors: Kim
authors: Yun-Gyoung
authors: YG
authors: Lee
authors: Oak-Kee
authors: OK
authors: Hong
authors: Hyuk-Sang
authors: HS
authors: Kwon
authors: Jong-Hoon
authors: JH
authors: Ji
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002062:Bursitis / Q000378:metabolism*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D012785:Shoulder Joint / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D018799:Intercellular Adhesion Molecule-1