title: Cyclic stretch augments production of neutrophil chemokines and matrix metalloproteinases-1 (MMP-1) from human decidual cells, and the production was reduced by progesterone.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the impact of mechanical stretch caused by uterine contraction and progesterone (P₄) on decidual cells (DC), neutrophil chemokines, and MMP-1 expression.
  
  
  METHODS
  DC were cultured, and cyclic stretch was applied using a computer-operated cell stretch system. Interleukin (IL)-8, growth-regulated oncogene (GRO) α, matrix metalloproteinase (MMP)-1, and mRNA and/or protein expression/activity was measured using RT-PCR and ELISA. Neutrophil chemotactic activity in conditioned media was evaluated using migration assays. The effect of P₄ was also studied.
  
  
  RESULTS
  Cyclic mechanical stretch increased IL-8, GROα, mRNA and protein, and MMP-1 production and activity level. Supernatant from stretched cells induced neutrophil chemotactic activity significantly. P₄ suppressed the effect of stretch.
  
  
  CONCLUSIONS
  The current study demonstrates that cyclic mechanical stretch stimulates the production of neutrophil chemokines and MMP-1 from human decidual cells, and the production was reduced by progesterone. These findings suggest that decidual cells are responding to mechanical and endocrine signals and induce biochemical factors and thereby contribute to the regulation of human labor.
authors: Obstetrics and Gynecology, The University of Tokyo, Japan.
authors: Yin
authors: Y
authors: Zhao
authors: Kaori
authors: K
authors: Koga
authors: Yutaka
authors: Y
authors: Osuga
authors: Gentaro
authors: G
authors: Izumi
authors: Masashi
authors: M
authors: Takamura
authors: Miyuki
authors: M
authors: Harada
authors: Tetsuya
authors: T
authors: Hirata
authors: Yasushi
authors: Y
authors: Hirota
authors: Osamu
authors: O
authors: Yoshino
authors: Satoshi
authors: S
authors: Inoue
authors: Tomoyuki
authors: T
authors: Fujii
authors: Shiro
authors: S
authors: Kozuma
mesh_terms: D002478:Cells, Cultured; D054360:Chemokine CXCL1 / Q000235:genetics / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003656:Decidua / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D040542:Mechanotransduction, Cellular / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D014590:Uterine Contraction / Q000276:immunology*
chemical_list: D054360:Chemokine CXCL1; D018925:Chemokines; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D012333:RNA, Messenger; D011374:Progesterone; D020781:Matrix Metalloproteinase 1