title: Purification and characterization of anti-HIV-1 protein from Canna indica L. leaves.
abstract: A novel 10 kDa protein with anti-HIV-1 reverse transcriptase (RT) inhibitory activity was isolated from leaves of Canna indica L. using a combination of native-PAGE and ammonium sulfate precipitation. HIV-1 and RT inhibitory activity was measured using a syncytium forming (deltaTat/Rev) MC99 virus in Tat/Rev transfected 1A2 cell line and ELISA technique, respectively. Edman N-terminal and internal amino acid sequence (using LC-MS-MS) determination revealed the 10 kDa Canna indica L. leaf protein as a putative plastocyanin. This is the first report of a plant plastocyanin with HIV-1 RT inhibitory property.
authors: Department of Pharmaceutical Science, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors: Apanchanid
authors: A
authors: Thepouyporn
authors: Chalobon
authors: C
authors: Yoosook
authors: Wongsatit
authors: W
authors: Chuakul
authors: Krit
authors: K
authors: Thirapanmethee
authors: Chanita
authors: C
authors: Napaswad
authors: Chanpen
authors: C
authors: Wiwat
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D015497:HIV-1 / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010970:Plastocyanin / Q000737:chemistry / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D019862:Zingiberales
chemical_list: D019380:Anti-HIV Agents; D010936:Plant Extracts; D010970:Plastocyanin; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase