title: Dexamethasone alters epithelium proliferation and survival and suppresses Wnt/β-catenin signaling in developing cleft palate.
abstract: Dexamethasone (Dex) contributes to a cleft palate, but the cellular and molecular mechanisms responsible for the deleterious effect on the developing palate are unclear. Wnt signaling is a causal mechanism of Dex-induced osteoporosis, so this study was conducted to determine whether Dex-induced cleft palate may result from altered Wnt signaling. Administration of Dex to mice completely inhibited canonical Wnt/β-catenin signaling and altered cell proliferation and apoptosis of the craniofacial epithelium in developing embryos. Thus, downregulated Wnt/β-catenin signaling was associated with Dex-induced cleft palate. Moreover, altered cell fate by Dex responsible for small palates, delaying shelf elevation and unfused palates was a crucial mechanism in cleft palate. Our findings help in elucidating the mechanisms of Dex-induced cleft palate.
authors: Department of Plastic and Cosmetic Surgery, Nan Fang Hospital, Guangzhou 510515, People's Republic of China.
authors: Xiao
authors: X
authors: Hu
authors: Jian Hua
authors: JH
authors: Gao
authors: Yun Jun
authors: YJ
authors: Liao
authors: Shi Jie
authors: SJ
authors: Tang
authors: Feng
authors: F
authors: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002972:Cleft Palate / Q000139:chemically induced / Q000196:embryology / Q000473:pathology*; D003907:Dexamethasone / Q000009:adverse effects*; D015536:Down-Regulation; D047108:Embryonic Development / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects* / Q000196:embryology; D005260:Female; D020287:In Situ Nick-End Labeling; D051379:Mice; D008855:Microscopy, Electron, Scanning; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D051176:beta Catenin; D003907:Dexamethasone