title: Chemical constituents from a soil-derived actinomycete, Actinomadura miaoliensis BCRC 16873, and their inhibitory activities on lipopolysaccharide-induced tumor necrosis factor production.
abstract: An investigation on the secondary metabolites from the BuOH extract of the fermentation broth of the thermotolerant polyester-degrading actinomycete Actinomadura miaoliensis BCRC 16873 was carried out. One previously undescribed α-pyrone (=pyran-2-one) derivative, designated as miaolienone (1), and a new butanolide, miaolinolide (2), together with 13 known compounds, 3-15, were obtained. Their structures were established on the basis of extensive 1D- and 2D-NMR analyses in combination with HR-MS experiments. In addition, the isolated compounds 1-15 were evaluated for the inhibitory effects of the isolates on the production of tumor necrosis factor (TNF-α) induced by lipopolysaccharide (LPS). Among the isolates, 1 and 2 significantly inhibited TNF-α production in U937 cells in vitro, and the IC(50) values were 0.59 and 0.76 μM, respectively. Compounds 3-5 displayed moderate inhibitory activities on LPS-induced TNF-α production.
authors: Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan.
authors: Min
authors: M
authors: Tseng
authors: Yung-Shun
authors: YS
authors: Su
authors: Ming-Jen
authors: MJ
authors: Cheng
authors: Ta-Wei
authors: TW
authors: Liu
authors: Ih-Sheng
authors: IS
authors: Chen
authors: Ming-Der
authors: MD
authors: Wu
authors: Hsun-Shuo
authors: HS
authors: Chang
authors: Gwo-Fang
authors: GF
authors: Yuan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology; D000192:Actinomycetales / Q000737:chemistry*; D002460:Cell Line; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012988:Soil Microbiology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000276:immunology
chemical_list: C582589:3-(hydroxymethyl)-6-(1-hydroxy-1-methyltridecyl)-4-methoxy-2H-pyran-2-one; D008070:Lipopolysaccharides; D011753:Pyrones; D014409:Tumor Necrosis Factor-alpha; C582590:dihydro-4-hydroxy-3-icosylidene-5-methylidenefuran-2(3H)-one; D015107:4-Butyrolactone