title: Lipopolysaccharide-induced inflammation of bronchi and emphysematous changes of pulmonary parenchyma in miniature pigs (Sus scrofa domestica).
abstract: Lipopolysaccharide (LPS) is widely used to induce chronic obstructive pulmonary disease (COPD) in animal models. Rodents are most commonly used to model COPD, but their substantial anatomic and physiological differences from humans present a challenge in the research of COPD pathogenesis. The authors induced COPD in miniature pigs by intratracheal administration of LPS solution. They carried out bronchoalveolar lavage (BAL) and collected the fluid for analyses of white blood cells, cytokines and proteases and obtained lung tissues for histological assessment. Intratracheal administration of LPS caused bronchitis, obstruction of distal bronchi and damage of pulmonary alveoli, as well as increases in white blood cell counts and expression levels of cytokines and proteases. These results are consistent with the presentation of COPD in humans, making LPS administration in miniature pigs a valuable animal model for the research of pathogenesis and treatment of COPD.
authors: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China.
authors: Piren
authors: P
authors: Chen
authors: Jiafa
authors: J
authors: Hou
authors: Dezhong
authors: D
authors: Ding
authors: Xiuguo
authors: X
authors: Hua
authors: Zhibiao
authors: Z
authors: Yang
authors: Li
authors: L
authors: Cui
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology; D000818:Animals; D001980:Bronchi / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology*; D004195:Disease Models, Animal; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D015846:Monokines / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000276:immunology / Q000473:pathology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature
chemical_list: D008070:Lipopolysaccharides; D015846:Monokines; D012333:RNA, Messenger; C482199:lipopolysaccharide, E coli O55-B5; D010450:Endopeptidases