title: Production of Monoclonal Antibody against Human Nestin.
abstract: We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays.
authors: Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors: Reza
authors: R
authors: Hadavi
authors: Amir Hassan
authors: AH
authors: Zarnani
authors: Negah
authors: N
authors: Ahmadvand
authors: Ahmad Reza
authors: AR
authors: Mahmoudi
authors: Ali Ahmad
authors: AA
authors: Bayat
authors: Jafar
authors: J
authors: Mahmoudian
authors: Mohammad-Reza
authors: MR
authors: Sadeghi
authors: Haleh
authors: H
authors: Soltanghoraee
authors: Mohammad Mehdi
authors: MM
authors: Akhondi
authors: Majid
authors: M
authors: Tarahomi
authors: Mahmood
authors: M
authors: Jeddi-Tehrani
authors: Hodjattallah
authors: H
authors: Rabbani
keywords: Antibodies; Blotting; Immunohistochemistry; Monoclonal; Nestin; Peptides; Western