title: Role of Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mice in vitro.
abstract: In a previous study, we have identified a set of conserved spermatogenic genes whose expression is restricted to testis and ovary and that are developmentally regulated. One of these genes, the transcription factor Mael, has been reported to play an essential role in mouse spermatogenesis. Nevertheless, the role of Mael in mouse oogenesis has not been defined. In order to analyse the role of Mael in mouse oogenesis, the expression of this gene was blocked during early oogenesis in mouse in vitro using RNAi technology. In addition, the role of Mael during differentiation of embryonic stem cells (ESC) into germ cells in vitro was analysed. Results show that downregulation of Mael by a specific short interfering RNA disrupted fetal oocyte growth and differentiation in fetal ovary explants in culture and the expression of several germ-cell markers in ESC during their differentiation. These results suggest that there is an important role for Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mouse in vitro.
authors: Department of Health Sciences,Universidad Autónoma Metropolitana-Iztapalapa,México DF 09340,México.
authors: I
authors: I
authors: Bahena
authors: Feinberg School of Medicine,Northwestern University,Chicago IL 60611,USA.
authors: E
authors: E
authors: Xu
authors: Department of Health Sciences,Universidad Autónoma Metropolitana-Iztapalapa,México DF 09340,México.
authors: M
authors: M
authors: Betancourt
authors: Department of Health Sciences,Universidad Autónoma Metropolitana-Iztapalapa,México DF 09340,México.
authors: E
authors: E
authors: Casas
authors: Department of Health Sciences,Universidad Autónoma Metropolitana-Iztapalapa,México DF 09340,México.
authors: Y
authors: Y
authors: Ducolomb
authors: Department of Health Sciences,Universidad Autónoma Metropolitana-Iztapalapa,México DF 09340,México.
authors: C
authors: C
authors: González
authors: Departamento de Ciencias de la Salud,Universidad Autónoma Metropolitana-Iztapalapa,Av. San Rafael Atlixco # 186,Col Vicentina,México DF 09340,México.
authors: E
authors: E
authors: Bonilla
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D008815:Mice, Inbred Strains; D009865:Oocytes; D009866:Oogenesis / Q000235:genetics*; D010053:Ovary / Q000166:cytology / Q000196:embryology; D034622:RNA Interference; D034741:RNA, Small Interfering; D046509:Tissue Culture Techniques; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; C531147:maelstrom protein, mouse