title: Genome-wide survey of interindividual differences of RNA stability in human lymphoblastoid cell lines.
abstract: The extent to which RNA stability differs between individuals and its contribution to the interindividual expression variation remain unknown. We conducted a genome-wide analysis of RNA stability in seven human HapMap lymphoblastoid cell lines (LCLs) and analyzed the effect of DNA sequence variation on RNA half-life differences. Twenty-six percent of the expressed genes exhibited RNA half-life differences between LCLs at a false discovery rate (FDR) < 0.05, which accounted for ~ 37% of the gene expression differences between individuals. Nonsense polymorphisms were associated with reduced RNA half-lives. In genes presenting interindividual RNA half-life differences, higher coding GC3 contents (G and C percentages at the third-codon positions) were correlated with increased RNA half-life. Consistently, G and C alleles of single nucleotide polymorphisms (SNPs) in protein coding sequences were associated with enhanced RNA stability. These results suggest widespread interindividual differences in RNA stability related to DNA sequence and composition variation.
authors: Center for Psychiatric Genetics, NorthShore University HealthSystem Research Institute. Evanston, Illinois 60201, USA. jduan@uchicago.edu
authors: Jubao
authors: J
authors: Duan
authors: Jianxin
authors: J
authors: Shi
authors: Xijin
authors: X
authors: Ge
authors: Lars
authors: L
authors: Dölken
authors: Winton
authors: W
authors: Moy
authors: Deli
authors: D
authors: He
authors: Sandra
authors: S
authors: Shi
authors: Alan R
authors: AR
authors: Sanders
authors: Jeff
authors: J
authors: Ross
authors: Pablo V
authors: PV
authors: Gejman
mesh_terms: D000328:Adult; D000483:Alleles; D000704:Analysis of Variance; D001482:Base Composition; D001483:Base Sequence; D018389:Codon, Nonsense; D019295:Computational Biology; D005260:Female; D014644:Genetic Variation; D015894:Genome, Human; D005838:Genotype; D006207:Half-Life; D060148:HapMap Project; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability
chemical_list: D018389:Codon, Nonsense; D012313:RNA