title: Two-stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity.
abstract: A cultivation system in the two-stage photoautotrophic-photoheterotrophic/mixotrophic mode was adapted to maximize lipid productivity of two freshwater strains of Chlorella sp. grown in brewery wastewater (BWW). The endogenous Chlorella sp. isolated from BWW had a higher growth rate than wild-type Chlorella vulgaris (UTEX-265) while C. vulgaris (UTEX-265) had a higher maximal biomass and lipid contents than that of endogenous Chlorella sp., resulting in more than 90% of the inorganic nutrients in both total nitrogen (TN) and phosphorus (TP) was removed during the first stage in the two-stage photoautotrophic-photoheterotrophic mode in each Chlorella sp. The maximal biomass and lipid contents of C. vulgaris (UTEX-265) for single stage photoautotrophic cultivation were 1.5 g/L and 18%, respectively. Importantly, during two-stage photoautotrophic-photoheterotrophic cultivation for C. vulgaris (UTEX-265), the biomass was increased to 3.5 g/L, and the lipid productivity was increased from 31.1 to 108.0mg/L day.
authors: Advanced Biomass R&D Center, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Wasif
authors: W
authors: Farooq
authors: Young-Chul
authors: YC
authors: Lee
authors: Byung-Gon
authors: BG
authors: Ryu
authors: Byung-Hyuk
authors: BH
authors: Kim
authors: Hee-Sik
authors: HS
authors: Kim
authors: Yoon-E
authors: YE
authors: Choi
authors: Ji-Won
authors: JW
authors: Yang
mesh_terms: D001515:Beer / Q000382:microbiology*; D056804:Biofuels; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000254:growth & development* / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D013045:Species Specificity; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D056804:Biofuels; D008055:Lipids