title: Markers of macrophage infiltration and measures of lipolysis in human abdominal adipose tissues.
abstract: OBJECTIVE
  We tested the hypothesis that high lipolytic responsiveness is related to increased expression of ATM genes in human adipose tissues.
  
  
  METHODS
  Omental (OM) and subcutaneous (SC) fat samples were obtained surgically in 46 women (age: 47.2 ± 4.7 years, BMI: 26.9 ± 5.2 kg/m(2) ). Body composition and fat distribution were measured using dual energy X-ray absorptiometry and computed tomography. Lipolysis was measured by glycerol release in mature adipocytes isolated by collagenase digestion under basal-, isoproterenol (10(-5) M)-, and forskolin (10(-5) M)-stimulated conditions. Quantification of macrophage gene mRNA expression (CD11b, CD11c, and CD68) in whole adipose tissue was performed using real-time RT-PCR.
  
  
  RESULTS
  SC CD68 mRNA abundance was positively associated with isoproterenol-stimulated lipolysis (r = 0.36, P < 0.05). This association remained significant after adjustment for total body fat mass (r = 0.34, P ≤ 0.05). In the OM depot, CD11b mRNA abundance was positively associated with isoproterenol-stimulated lipolysis (r = 0.42, P ≤ 0.005). This association remained significant after adjustment for total body fat mass (r = 0.41, P ≤ 0.01). In subgroup analyses, high lipolytic rates in SC adipocytes were related to increased whole tissue expression of CD68 and CD11b in this compartment, independent of adiposity and fat cell size (P ≤ 0.001 and P ≤ 0.05). High lipolytic rates in OM adipocytes were related to increased whole tissue OM expression of CD11b, independent of adiposity and fat cell size (P ≤ 0.05).
  
  
  CONCLUSIONS
  High adipocyte lipolytic responsiveness is related to increased expression of ATM markers in the corresponding compartment, independent of adiposity and fat cell size.
authors: Endocrinology and Genomics, Laval University Medical Center, 2705 Laurier Blvd. (T3-67), Québec G1V 4G2, Canada; Department of Nutrition, Laval University, Pavillon Paul Comtois, Québec G1K 7P4, Canada.
authors: Andréanne
authors: A
authors: Michaud
authors: Mélissa
authors: M
authors: Pelletier
authors: Suzanne
authors: S
authors: Noël
authors: Céline
authors: C
authors: Bouchard
authors: André
authors: A
authors: Tchernof
mesh_terms: D050153:Abdominal Fat / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D018845:CD11 Antigens / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects / Q000235:genetics*; D008264:Macrophages / Q000502:physiology*; D008875:Middle Aged; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D064007:Ataxia Telangiectasia Mutated Proteins; D015415:Biomarkers; D018845:CD11 Antigens; D005990:Glycerol; D007545:Isoproterenol; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C576324:ATM protein, human