title: Comprehensive analysis of ß-lactam antibiotics including penicillins, cephalosporins, and carbapenems in poultry muscle using liquid chromatography coupled to tandem mass spectrometry.
abstract: A comprehensive method for the quantitative residue analysis of trace levels of 22 ß-lactam antibiotics, including penicillins, cephalosporins, and carbapenems, in poultry muscle by liquid chromatography in combination with tandem mass spectrometric detection is reported. The samples analyzed for ß-lactam residues are hydrolyzed using piperidine in order to improve compound stability and to include the total residue content of the cephalosporin ceftifour. The reaction procedure was optimized using a full experimental design. Following detailed isotope labeling, tandem mass spectrometry studies and exact mass measurements using high-resolution mass spectrometry reaction schemes could be proposed for all ß-lactams studied. The main reaction occurring is the hydrolysis of the ß-lactam ring under formation of the piperidine substituted amide. For some ß-lactams, multiple isobaric hydrolysis reaction products are obtained, in accordance with expectations, but this did not hamper quantitative analysis. The final method was fully validated as a quantitative confirmatory residue analysis method according to Commission Decision 2002/657/EC and showed satisfactory quantitative performance for all compounds with trueness between 80 and 110% and within-laboratory reproducibility below 22% at target level, except for biapenem. For biapenem, the method proved to be suitable for qualitative analysis only.
authors: RIKILT, Wageningen University and Research Centre, Akkermaalsbos 2, 6708WB, P.O. Box 230, 6700AE, Wageningen, The Netherlands, bjorn.berendsen@wur.nl.
authors: Bjorn J A
authors: BJ
authors: Berendsen
authors: Henk W
authors: HW
authors: Gerritsen
authors: Robin S
authors: RS
authors: Wegh
authors: Steven
authors: S
authors: Lameris
authors: Ralph
authors: R
authors: van Sebille
authors: Alida A M
authors: AA
authors: Stolker
authors: Michel W F
authors: MW
authors: Nielen
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D015780:Carbapenems / Q000737:chemistry*; D002511:Cephalosporins / Q000032:analysis / Q000737:chemistry; D002853:Chromatography, Liquid; D057230:Limit of Detection; D018482:Muscle, Skeletal / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis / Q000737:chemistry; D011200:Poultry; D053719:Tandem Mass Spectrometry; D047090:beta-Lactams / Q000032:analysis / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D015780:Carbapenems; D002511:Cephalosporins; D010406:Penicillins; D047090:beta-Lactams; C053503:ceftiofur