title: MiR-106a is an independent prognostic marker in patients with glioblastoma.
abstract: BACKGROUND
  Very little is known regarding correlation of micro RNA (miR)-106a with clinical outcomes of patients with glioblastoma multiforme (GBM). This study determined whether miR-106a could be used as an independent prognostic biomarker in those patients.
  
  
  METHODS
  A total of 156 GBM patients were divided into 2 cohorts. In the first cohort, matched fresh frozen and formalin-fixed paraffin-embedded (FFPE) samples were collected from 24 GBM patients, while in the second cohort, only FFPE samples were collected from 132 GBM patients. MiR-106a expression levels were examined by quantitative real-time PCR in the 2 cohorts and further validated by in situ hybridization assay in the second cohort. The correlation between miR-106a expression levels and overall survival was evaluated in the second cohort of 114 GBM patients available for follow-up by a log-rank test and a multivariate Cox proportional hazards model.
  
  
  RESULTS
  Our data showed a very good correlation of miR-106a or U6 expression between fresh frozen and FFPE GBM specimens, with Pearson's correlation coefficients of 0.849 and 0.823, respectively (P < .001). Their expression levels in archival FFPE samples were quite stable for at least 7 years when stored at room temperature. Multivariate analysis revealed that the expression level of miR-106a was an independent and significant predictor of overall survival in GBM patients (P = .011).
  
  
  CONCLUSIONS
  MiR-106a expression was relatively abundant and stable in a large cohort of archival FFPE GBM specimens and could be used as an independent prognostic biomarker in those patients. Thus, miR-106a can be used to predict prognosis and treatment response in individual GBM patients.
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, People's Republic of China. guangsz@hotmail.com
authors: Shiguang
authors: S
authors: Zhao
authors: Guang
authors: G
authors: Yang
authors: Yulong
authors: Y
authors: Mu
authors: Dayong
authors: D
authors: Han
authors: Chen
authors: C
authors: Shi
authors: Xin
authors: X
authors: Chen
authors: Yifan
authors: Y
authors: Deng
authors: Daming
authors: D
authors: Zhang
authors: Lu
authors: L
authors: Wang
authors: Yaohua
authors: Y
authors: Liu
authors: Xu
authors: X
authors: Hou
authors: Chunlei
authors: C
authors: Wang
authors: Jianing
authors: J
authors: Wu
authors: Huailei
authors: H
authors: Liu
authors: Ligang
authors: L
authors: Wang
authors: Guang
authors: G
authors: Zhang
authors: Jiping
authors: J
authors: Qi
authors: Xiang
authors: X
authors: Fang
authors: Changbin
authors: C
authors: Shi
authors: Jing
authors: J
authors: Ai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality; D002648:Child; D005260:Female; D005909:Glioblastoma / Q000235:genetics* / Q000401:mortality; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: FFPE; glioblastoma; miR-106a; prognosis; prognostic marker