title: Tenogenic differentiation of equine adipose-tissue-derived stem cells under the influence of tensile strain, growth differentiation factors and various oxygen tensions.
abstract: Mesenchymal stem cells have become extremely interesting for regenerative medicine and tissue engineering in the horse. Stem cell therapy has been proven to be a powerful and successful instrument, in particular for the healing of tendon lesions. We pre-differentiated equine adipose-tissue-derived stem cells (ASCs) in a collagen I gel scaffold by applying tensile strain, growth differentiation factors (GDFs) and various oxygen tensions in order to determine the optimal conditions for in vitro differentiation toward the tenogenic lineage. We compared the influence of 3% versus 21% oxygen tension, the use of GDF 5, GDF 6 and GDF 7 and the application of uniaxial tensile strain versus no mechanical stimulation on differentiation results as evaluated by cell morphology and by the expression of the tendon-relevant genes collagen I, collagen III, cartilage oligomeric matrix protein and scleraxis. The best results were obtained with an oxygen tension of 21%, tensile stimulation and supplementation with GDF 5 or GDF 7. This approach raises the hope that the in vivo application of pre-differentiated stem cells will improve healing and recovery time in comparison with treatment involving undifferentiated stem cells.
authors: Institute of Veterinary Anatomy, Histology, and Embryology, Justus-Liebig University of Giessen, Frankfurterstrasse 98, 35392, Giessen, Germany. Oksana.Raabe@vetmed.uni-giessen.de
authors: Oksana
authors: O
authors: Raabe
authors: K
authors: K
authors: Shell
authors: D
authors: D
authors: Fietz
authors: C
authors: C
authors: Freitag
authors: A
authors: A
authors: Ohrndorf
authors: H J
authors: HJ
authors: Christ
authors: S
authors: S
authors: Wenisch
authors: S
authors: S
authors: Arnhold
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019149:Bioreactors; D002450:Cell Communication / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D024042:Collagen Type I / Q000494:pharmacology; D005782:Gels; D005786:Gene Expression Regulation / Q000187:drug effects; D055412:Growth Differentiation Factors / Q000494:pharmacology*; D006736:Horses; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D013710:Tendons / Q000166:cytology*; D013718:Tensile Strength / Q000187:drug effects*; D054457:Tissue Scaffolds
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D005782:Gels; D055412:Growth Differentiation Factors; D012333:RNA, Messenger; D010100:Oxygen