title: Xylosylation as an effective means for reducing yeast growth inhibition by 2-phenylethanol.
abstract: Microbial bioproduction processes of 2-phenylethanol, an important rose-like flavor and fragrance compound that occurs naturally in the essential oils of many flowers and plants, are hindered by the growth inhibition it exerts towards the producing microorganism, mainly yeast. We show here for the first time that glycosylation of 2-phenylethanol with xylose increased the inhibitory concentration inducing 50% decrease of the yeast Saccharomyces cerevisiae strain BY4741 growth rate (IC50 ) from 14 mM (1.71 g/L) 2-phenylethanol (2PE) to 100 mM (25.5 g/L) 2-phenylethyl β-D-xylopyranoside (X-2PE). More interestingly, the IC10 was only 3 mM (0.37 g/L) for 2PE and 86 mM (21.9 g/L) for X-2PE. Xylosylation of 2-phenylethanol can offer therefore an effective means for reducing yeast growth inhibition in microbial bioproduction processes of this important flavor and fragrance compound.
authors: UniversitédeToulouse; INSA, UPS, INP; LISBP, Toulouse, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, Toulouse, France; CNRS, UMR5504, Toulouse, France.
authors: Haojun
authors: H
authors: Zhang
authors: Régis
authors: R
authors: Fauré
authors: Jean M
authors: JM
authors: François
authors: Philippe J
authors: PJ
authors: Blanc
authors: Gustavo M
authors: GM
authors: de Billerbeck
mesh_terms: D001709:Biotechnology / Q000379:methods; D006031:Glycosylation; D006131:Growth Inhibitors / Q000737:chemistry / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D010626:Phenylethyl Alcohol / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D014994:Xylose
chemical_list: D006131:Growth Inhibitors; D014994:Xylose; D010626:Phenylethyl Alcohol
keywords: Flavors; Fragrances; Glycosylation; Growth inhibition; Phenylethanol; Saccharomyces cerevisiae