title: Carotenoids and phenolic compounds from Solanum sessiliflorum, an unexploited Amazonian fruit, and their scavenging capacities against reactive oxygen and nitrogen species.
abstract: The composition of carotenoids and phenolic compounds from mana-cubiu (Solanum sessiliflorum), a fruit native to Amazonia, was determined by high-performance liquid chromatography coupled to diode array and mass spectrometry detectors (HPLC-DAD-MS(n)). The antioxidant capacities of the hydrophilic and carotenoid extracts against some reactive oxygen (ROO(•), H(2)O(2), HOCl, and HO(•)) and nitrogen (ONOO(-)) species were also determined. Seventeen carotenoids and three phenolic compounds were found in mana-cubiu. The major carotenoids were (all-E)-β-carotene (7.15 μg/g of dry weight) and (all-E)-lutein (2.41 μg/g of dry weight). The 5-caffeoylquinic acid (1351 μg/g of dry weight) was the major phenolic compound, representing more than 78% (w/w) of the total phenolic compounds. Moreover, two dihydrocaffeoyl spermidines were found in the hydrophilic extract. Both mana-cubiu extracts were able to scavenge all the tested reactive species. The carotenoid extract was shown to be a potent scavenger of peroxyl radical, while the hydrophilic extract was a potent hydrogen peroxide and hypochlorous acid scavenger.
authors: Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP) , 13083-862 Campinas-SP, Brazil.
authors: Eliseu
authors: E
authors: Rodrigues
authors: Lilian R B
authors: LR
authors: Mariutti
authors: Adriana Z
authors: AZ
authors: Mercadante
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry; D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006997:Hypochlorous Acid / Q000737:chemistry; D014975:Lutein / Q000032:analysis / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D030421:Peroxynitrous Acid / Q000737:chemistry; D019800:Phenol / Q000032:analysis* / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D026361:Reactive Nitrogen Species / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D032322:Solanum / Q000737:chemistry*; D019207:beta Carotene / Q000032:analysis / Q000737:chemistry
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010545:Peroxides; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; C472707:caffeoylquinic acid; D019207:beta Carotene; D011801:Quinic Acid; D030421:Peroxynitrous Acid; C049375:perhydroxyl radical; D019800:Phenol; D002338:Carotenoids; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D014975:Lutein