title: Inactivation of Escherichia coli O157:H7 on cattle hides by caprylic acid and β-resorcylic acid.
abstract: Two naturally occurring, generally recognized as safe compounds, namely, caprylic acid (CA) (1%) and b -resorcylic acid (BR) (1%), and their combination, applied at 23 and 60°C were evaluated for their antimicrobial effects against Escherichia coli O157:H7 on cattle hides  in the presence and absence of bovine feces. Fresh cleaned cattle hides were cut into pieces (5 cm(2)), air dried, and inoculated with a five-strain mixture of nalidixic acid-resistant (50 μg/ml) E. coli O157:H7 (∼8.0 log CFU). The hide samples were air dried under  a biosafety hood for 2 h and sprayed with 95% ethanol, 1% CA, 1% BR, or a mixture of 1% CA and 1% BR at 23 or 60°C. The hide samples were kept at 23°C, and E. coli O157:H7 populations were determined at 2 and 5 min after treatment. Both CA and BR were effective in decreasing  E. coli O157:H7 populations on hides by 3 to 4 log CFU/cm(2) (P < 0.05). Sterile bovine feces had no effect on the decontaminating property of CA and BR on cattle hides (P > 0.05). Results of this study indicate that CA and BR could potentially be used to  decontaminate cattle hides, but follow-up research under slaughterhouse conditions is warranted.
authors: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors: Sangeetha Ananda
authors: SA
authors: Baskaran
authors: Varunkumar
authors: V
authors: Bhattaram
authors: Indu
authors: I
authors: Upadhyaya
authors: Abhinav
authors: A
authors: Upadhyay
authors: Anup
authors: A
authors: Kollanoor-Johny
authors: David
authors: D
authors: Schreiber
authors: Kumar
authors: K
authors: Venkitanarayanan
mesh_terms: D000003:Abattoirs; D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D002417:Cattle / Q000382:microbiology*; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D019453:Escherichia coli O157 / Q000187:drug effects*; D005243:Feces / Q000382:microbiology; D062385:Hydroxybenzoates / Q000494:pharmacology*; D013696:Temperature
chemical_list: D002210:Caprylates; D004202:Disinfectants; D062385:Hydroxybenzoates; C017704:beta-resorcylic acid; C031492:octanoic acid