title: Array-CGH analysis of cutaneous anaplastic large cell lymphoma.
abstract: This chapter describes a study in which the pattern of numerical chromosomal alterations in cutaneous anaplastic large cell lymphoma (C-ALCL) tumor samples was defined using array-based comparative genomic hybridization (CGH). First, the array-based CGH technique applied is outlined in detail. Next, its application in the analysis of C-ALCL tumor specimens is described. This approach resulted in the identification of highly recurrent chromosomal alterations in C-ALCL that include gain of 7q31 and loss on 6q16-6q21 and 13q34, each affecting 45% of the patients. The pattern characteristic of C-ALCL differs markedly from chromosomal alterations observed in other CTCL such as mycosis fungoides and Sézary syndrome and yielded several candidate genes with potential relevance in the pathogenesis of C-ALCL.
authors: Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands.
authors: K
authors: K
authors: Szuhai
authors: R
authors: R
authors: van Doorn
authors: C P
authors: CP
authors: Tensen
authors: Van Kester
mesh_terms: D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D017728:Lymphoma, Large-Cell, Anaplastic / Q000235:genetics*; D016410:Lymphoma, T-Cell, Cutaneous / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012878:Skin Neoplasms / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004247:DNA