title: Effect of 4-methylpyrazole on antioxidant enzyme status and lipid peroxidation in the liver of rats after exposure to ethylene glycol and ethyl alcohol.
abstract: BACKGROUND
  The aim of the conducted studies was to evaluate the effect of 4-methylpyrazole, increasingly used in detoxifying treatments after ethylene glycol poisoning, on the activity of some antioxidant enzymes and lipid peroxidation formation in the liver of rats after experimental co-exposure to ethylene glycol and ethyl alcohol.
  
  
  METHODS
  The trials were conducted on adult male Wistar rats. Ethylene glycol (EG) at the dose of 3.83 g/kg bw and ethyl alcohol (EA) at the dose of 1 g/kg bw were administered po, and 4-methylpyrazole (4-MP) at the dose of 0.01 g/kg bw was administered ip. Parameters of antioxidant balance were evaluated in hepatic cytosol, including the activity of the following enzymes: glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation level (TBARS).
  
  
  RESULTS
  The results suggest that evaluation of the effects of administrated 4-MP after co-exposure to EG and EA in the liver revealed statistically significant changes on antioxidant enzyme system and malondialdehyde formation.
  
  
  CONCLUSIONS
  The changes in biomarkers activity indicate a greater production of free radicals which exceeds the capability of antioxidant system, appearing with oxidative stress in the group of animals treated by 4-MP combined with EG and EA.
authors: Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, PL 60-631 Poznań, Poland. karina.sommerfeld@gmail.com
authors: Karina
authors: K
authors: Sommerfeld
authors: Barbara
authors: B
authors: Zielińska-Psuja
authors: Jędrzej
authors: J
authors: Przystanowicz
authors: Joanna
authors: J
authors: Kowalówka-Zawieja
authors: Jerzy
authors: J
authors: Orłowski
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D004798:Enzymes / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D019855:Ethylene Glycol / Q000008:administration & dosage / Q000633:toxicity*; D000077604:Fomepizole; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011720:Pyrazoles / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D004798:Enzymes; D011720:Pyrazoles; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; D008315:Malondialdehyde; D000077604:Fomepizole; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D019855:Ethylene Glycol