title: Characterization of neuron-like cells derived from canine bone marrow stromal cells.
abstract: Regenerative therapy using bone marrow stromal cells (BMSCs) has begun to be clinically applied in humans and dogs for neurological disorders such as spinal cord injury. Under appropriate conditions in vitro, BMSCs differentiate into neuronal cells, which may improve the effects of regenerative therapy. In this study, we evaluated canine neuron-like cells (NLCs) derived from BMSCs. We speculated on their suitability for neuro-transplantation from the point of view of their morphological features, long-term viability, abundant availability, and ability to be subcultured. Canine NLCs were differentiated as follows: third-passage BMSCs were maintained in pre-induction medium containing 2-mercaptoethanol and dimethylsulfoxide for 5 h, and then cells were transferred to neuronal induction medium containing fetal bovine serum, basic fibroblast growth factor, epidermal growth factor, dibutyryl cyclic AMP, and isobutylmethylxanthine for 7 or 14 days. Canine NLCs fulfilled the transplantation criteria and expressed markers of both immature neurons (nestin, 84.7 %) and mature neuronal cells (microtubule-associated protein-2, 95.7 %; βIII-tubulin protein, 12.9 %; glial fibrillary acidic protein, 9.2 %). These results suggest that canine BMSCs can be induced to differentiate into neuronal cells and may be suitable for neuro-transplantation. This study may provide information for improving cellular therapy for neurological diseases.
authors: Department of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan. m003tm@yamaguchi-u.ac.jp
authors: Yasutaka
authors: Y
authors: Oda
authors: Kenji
authors: K
authors: Tani
authors: Toshitaka
authors: T
authors: Kanei
authors: Tomoya
authors: T
authors: Haraguchi
authors: Kazuhito
authors: K
authors: Itamoto
authors: Hiroshi
authors: H
authors: Nakazawa
authors: Yasuho
authors: Y
authors: Taura
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000662:veterinary; D002454:Cell Differentiation / Q000502:physiology; D004285:Dogs / Q000033:anatomy & histology* / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000662:veterinary; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D044968:Regenerative Medicine / Q000379:methods; D014404:Tubulin / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D064231:Nestin; D014404:Tubulin