title: Screening ADAMTS10 in dog populations supports Gly661Arg as the glaucoma-causing variant in beagles.
abstract: OBJECTIVE
  Previously, we mapped the disease locus in the beagle model of autosomal recessive primary open angle glaucoma (POAG) to a 4-Mb interval on chromosome 20, and identified a Gly661Arg variant in ADAMTS10 as the candidate disease-causing variant. The purpose of this study was to test the hypothesis that the Gly661Arg variant of ADAMTS10 causes glaucoma by genotyping dogs of various breeds affected and unaffected by primary glaucoma.
  
  
  METHODS
  Dogs of various breeds, affected or unaffected with primary glaucoma, were genotyped for the Gly661Arg variant of ADAMTS10, as well as 7 other nonsynonymous single nucleotide polymorphisms (SNPs) in other genes in the beagle POAG locus that segregate with disease. Alternate allele frequencies were calculated with 95% confidence intervals and comparisons made to expected allele frequency relative to disease prevalence or between cases and controls.
  
  
  RESULTS
  For the nonsynonymous SNPs other than the ADAMTS10 variant, control dogs were identified that were homozygous for the alternative alleles, ruling out those variants as causative. None of the nonsynonymous SNPs were found associated with primary glaucoma in American cocker spaniels. The Gly661Arg variant of ADAMTS10 was the only variant with minor allele frequency consistent with the prevalence of primary glaucoma in the general beagle population. The only dog found homozygous for the Gly661Arg variant of ADAMTS10 was an affected beagle, unrelated to the POAG colony.
  
  
  CONCLUSIONS
  These findings support the Gly661Arg mutation of ADAMTS10 as the likely cause of POAG in beagles.
authors: Vanderbilt Eye Institute, Vanderbilt University, Nashville, Tennessee 37232, USA.
authors: John
authors: J
authors: Kuchtey
authors: Jessica
authors: J
authors: Kunkel
authors: Douglas
authors: D
authors: Esson
authors: John S
authors: JS
authors: Sapienza
authors: Daniel A
authors: DA
authors: Ward
authors: Caryn E
authors: CE
authors: Plummer
authors: Kirk N
authors: KN
authors: Gelatt
authors: Rachel W
authors: RW
authors: Kuchtey
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000071096:ADAMTS Proteins; D000483:Alleles; D000818:Animals; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis; D004285:Dogs; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods*; D005838:Genotype; D005902:Glaucoma, Open-Angle / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D007429:Intraocular Pressure; D009154:Mutation; D020641:Polymorphism, Single Nucleotide
chemical_list: D004247:DNA; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; C494614:ADAMTS10 protein, human