title: α-Glucosidase and α-amylase inhibitory activity of Senna surattensis.
abstract: In this study, we investigated the inhibitory effects of ethanolic extract of the leaves of Senna surattensis (EESS) on α-glucosidase and α-amylase. We also studied the in vitro antidiabetic activity of S. surattensis using the glucose uptake by isolated rat hemidiaphragm model. In vitro studies using mammalian α-glucosidase extracted from the small intestine homogenate of mouse showed that the extract was found to be more effective in inhibiting the activities of maltase [half maximal inhibitory concentration (IC(50)): 209.15 μg/mL] and sucrase (IC(50): 366.44 μg/mL) when compared with the control group (acarbose). The extract of S. surattensis were further quantified with respect to porcine pancreatic α-amylase inhibition using the chromogenic 3,5-dinitrosalicylic acid method. Interestingly, S. surattensis was also found to exhibit α-amylase (IC(50): 123.95 μg/mL) inhibitory activity. The glucose uptake in the rat hemidiaphragm was significantly (p < 0.01) increased by EESS (220.95 ± 5.4 mg/g/30 minute) when compared with the control group. The total polyphenolic content of EESS was found to be 98 μg pyrocatechol/mg of the extract. These results suggest that EESS inhibited carbohydrate digestive enzymes and increased the peripheral uptake of glucose. This study endorses the use of this plant for further studies to determine their potential for managing type II diabetes.
authors: Department of Pharmaceutical Technology, Division of Pharmacognosy, Faculty of Engineering and Technology, Jadavpur University, Kolkata, India. thilagampharma@gmail.com
authors: Ellappan
authors: E
authors: Thilagam
authors: Balasubramaian
authors: B
authors: Parimaladevi
authors: Chidambaram
authors: C
authors: Kumarappan
authors: Subhash Chandra
authors: SC
authors: Mandal
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000008:administration & dosage* / Q000032:analysis; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000032:analysis; D029846:Senna Plant / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases