title: Poly-(ethylene glycol) modified gelatin nanoparticles for sustained delivery of the anti-inflammatory drug Ibuprofen-Sodium: an in vitro and in vivo analysis.
abstract: The limited bioavailability and rapid clearance of the anti-inflammatory drug Ibuprofen Sodium (IbS) necessitates repeated drug administration. To address this, injectable IbS loaded PEGylated gelatin nanoparticles (PIG NPs) of size ~200nm and entrapment efficiency ~70%, providing sustained release in vitro were prepared by a modified two-step desolvation process. The developed nanomedicine, containing a range of IbS concentrations up to 1mg/mL proved to be non-toxic, hemocompatible and non-immunogenic, when tested through various in vitro assays and was reaffirmed by in vivo cytokine analysis. HPLC analysis of intravenously administered PIG NPs showed a sustained release of IbS for ~4days with improved bioavailability and pharmacokinetics when compared to bare IbS and IbS-loaded non-PEGylated GNPs. Histological analysis of liver and kidney revealed tissue integrity as in the control, indicating biocompatibility of PIG NPs. The results demonstrate improved plasma half-life of IbS when encapsulated within nanogelatin, thereby aiding reduction in its frequency of administration.
  
  
  BACKGROUND
  In this preclinical study, improved plasma half-life of ibuprofen sodium was demonstrated when encapsulated within PEGylated gelatin nanoparticles of ~200 nm size, expected to lead to reduced frequency of administration in future clinical applications.
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India.
authors: Dhanya
authors: D
authors: Narayanan
authors: Geena
authors: G
authors: M G
authors: Lakshmi
authors: L
authors: H
authors: Manzoor
authors: M
authors: Koyakutty
authors: Shantikumar
authors: S
authors: Nair
authors: Deepthy
authors: D
authors: Menon
mesh_terms: D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems; D005780:Gelatin / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D007052:Ibuprofen / Q000008:administration & dosage* / Q000737:chemistry; D007249:Inflammation / Q000188:drug therapy*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D011092:Polyethylene Glycols; D005780:Gelatin; D007052:Ibuprofen
keywords: Gelatin nanoparticles; Hemocompatibility; Ibuprofen Sodium; Pharmacokinetics; Poly(ethylene glycol)