title: Comparison of different methods of intestinal obstruction in a rat model.
abstract: OBJECTIVE
  To investigate different methods of creating incomplete intestinal obstruction in a rat model and to compare their electrophysiologic, morphologic and histologic characteristics.
  
  
  METHODS
  Rat ileum was partially obstructed by the respective application of: braided silk (penetrated the mesentery and surrounded intestine); half ligation (penetrated directly and ligated 1/2 cross-section of the intestine); wide pipe (6 mm in width, surrounded the intestine); narrow pipe (2 mm in width, surrounded the intestine). A control was also included (no obstruction). Various behavioral and electrophysiologic variables, as well as morphologic and immunohistochemical observations were recorded by blinded investigators at different time points (12, 24, 48, 72 h), including daily general condition, ileal wet weight and circumference, macromorphous and micromorphous intestine, bowel movement capability in vivo and in vitro, slow wave and neural electrical activity, and the number of c-Kit positive interstitial cells of Cajal (ICC).
  
  
  RESULTS
  Despite being of a similar general condition, these methods resulted in different levels of obstruction in each group compared with the control at different time points (12, 24, 48, 72 h). However, these fields of the wide pipe rat showed significantly differences when compared with the other three obstructed groups at 12 to 72 h, including macroscopic and histological presentation, intestinal transit ratio and contractility, circumference and wet weight, amplitude and frequency of nerve electrical discharge and slow wave, and ICC numbers (all P < 0.01).
  
  
  CONCLUSIONS
  The wide pipe rat method is significantly more reliable and stable than the other methods of obstruction, demonstrating that use of the wide pipe method can be a useful model of incomplete intestinal obstruction.
authors: Department of Clinical Medicine, Chengdu Medical College, Chengdu 610081, Sichuan Province, China.
authors: Meng-Lang
authors: ML
authors: Yuan
authors: Zheng
authors: Z
authors: Yang
authors: Yu-Cheng
authors: YC
authors: Li
authors: Lan-Lan
authors: LL
authors: Shi
authors: Jia-Ling
authors: JL
authors: Guo
authors: Yu-Qin
authors: YQ
authors: Huang
authors: Xia
authors: X
authors: Kang
authors: Jing-Jing
authors: JJ
authors: Cheng
authors: Yang
authors: Y
authors: Chen
authors: Ting
authors: T
authors: Yu
authors: De-Qi
authors: DQ
authors: Cao
authors: Huan
authors: H
authors: Pang
authors: Xiao
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005772:Gastrointestinal Transit; D007077:Ileal Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007082:Ileum / Q000294:innervation / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D056885:Interstitial Cells of Cajal / Q000378:metabolism; D007415:Intestinal Obstruction / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008026:Ligation; D008297:Male; D016065:Myoelectric Complex, Migrating; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D015415:Biomarkers; D019009:Proto-Oncogene Proteins c-kit
keywords: Comparative study; Electrophysiology; Interstitial cells of Cajal; Intestinal obstruction; Model; Morphology