title: Stable isotope labeling in zebrafish allows in vivo monitoring of cardiac morphogenesis.
abstract: Quantitative proteomics is an important tool to study biological processes, but so far it has been challenging to apply to zebrafish. Here, we describe a large scale quantitative analysis of the zebrafish proteome using a combination of stable isotope labeling and liquid chromatography-mass spectrometry (LC-MS). Proteins derived from the fully labeled fish were used as a standard to quantify changes during embryonic heart development. LC-MS-assisted analysis of the proteome of activated leukocyte cell adhesion molecule zebrafish morphants revealed a down-regulation of components of the network required for cell adhesion and maintenance of cell shape as well as secondary changes due to arrest of cellular differentiation. Quantitative proteomics in zebrafish using the stable isotope-labeling technique provides an unprecedented resource to study developmental processes in zebrafish.
authors: §Max-Planck-Institute for Heart and Lung Research, Ludwigstrasse 43, 61231 Bad Nauheim, Germany.
authors: Anne
authors: A
authors: Konzer
authors: Aaron
authors: A
authors: Ruhs
authors: Helene
authors: H
authors: Braun
authors: Benno
authors: B
authors: Jungblut
authors: Thomas
authors: T
authors: Braun
authors: Marcus
authors: M
authors: Krüger
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D048430:Cell Shape; D002853:Chromatography, Liquid; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D007553:Isotope Labeling; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D013058:Mass Spectrometry; D009024:Morphogenesis / Q000235:genetics*; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D029941:Fish Proteins; D020543:Proteome