title: A novel near-infrared fluorescent probe for selectively sensing nitroreductase (NTR) in an aqueous medium.
abstract: A novel near-infrared (NIR) fluorescent probe based on cyanine dyes was designed and synthesized to detect nitroreductase (NTR) activity. Upon reaction with NTR, the nitro-group of p-nitrobenzyl moiety was reduced to amino-group and therefore renews the π-electron conjugation of the dye, resulting in the intense NIR fluorescent emission (708 nm). This redox-induced NIR-fluorescence probe has been successfully applied to the detection of NTR activity under physiological conditions with high selectivity.
authors: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, PR China.
authors: Youmin
authors: Y
authors: Shi
authors: Sichun
authors: S
authors: Zhang
authors: Xinrong
authors: X
authors: Zhang
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007700:Kinetics; D009601:Nitroreductases / Q000032:analysis*; D019265:Spectroscopy, Near-Infrared / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D009601:Nitroreductases