title: Isolation and identification of antioxidative peptides from pilot-scale black-bone silky fowl (Gallus gallus domesticus Brisson) muscle oligopeptides.
abstract: BACKGROUND
  A pilot-scale production was developed to produce oligopeptide powder from black-bone silky fowl (Gallus gallus domesticus Brisson) muscle (BSFP) by two-step enzymatic hydrolysis and multistage separation. The resultant BSFP was assessed for antioxidant activities against four free radicals (hydroxyl, 1,1-dipheny-2-picrylhydrazyl (DPPH), superoxide and peroxyl) and against the peroxidation of linoleic acid in a lipid peroxidation model system. After separation by reversed-phase high-performance liquid chromatography (RP-HPLC), five major fractions of BSFP were tested for DPPH radical scavenging activity and subjected to mass spectrometry to identify the active peptides.
  
  
  RESULTS
  BSFP showed potential antioxidant activity in four assay systems. Three RP-HPLC fractions produced higher antioxidant effect than BSFP, with Fraction 4 showing the strongest activity. A total of 18 peptides were identified, and two peptides - Leu-Trp-Arg and Asn-Met - had strong scavenging activity, with IC50 values of 2.28 ± 0.05 and 4.65 ± 0.09 mg mL(-1) , respectively. Asn-Met is a novel antioxidative peptide that has not been previously reported.
  
  
  CONCLUSIONS
  The results showed that the pilot-scale production of BSFP was a practical way to produce peptides with high value and potential antioxidant activity. BSFP and its antioxidative peptides can be a source of natural antioxidant and used as a food additive.
authors: China National Research Institute of Food and Fermentation Industries, Beijing, 100027, People's Republic of China.
authors: Wenying
authors: W
authors: Liu
authors: Ruizeng
authors: R
authors: Gu
authors: Feng
authors: F
authors: Lin
authors: Jun
authors: J
authors: Lu
authors: Weixue
authors: W
authors: Yi
authors: Yong
authors: Y
authors: Ma
authors: Zhe
authors: Z
authors: Dong
authors: Muyi
authors: M
authors: Cai
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D015227:Lipid Peroxidation; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D010865:Pilot Projects; D011208:Powders; D013481:Superoxides
chemical_list: D000975:Antioxidants; D009842:Oligopeptides; D011208:Powders; D013481:Superoxides
keywords: antioxidant peptide; black-bone silky fowl; enzymatic hydrolysis; low-molecular-weight peptides; pilot-scale production