title: BEST-TROSY experiments for time-efficient sequential resonance assignment of large disordered proteins.
abstract: The characterization of the conformational properties of intrinsically disordered proteins (IDPs), and their interaction modes with physiological partners has recently become a major research topic for understanding biological function on the molecular level. Although multidimensional NMR spectroscopy is the technique of choice for the study of IDPs at atomic resolution, the intrinsically low resolution, and the large peak intensity variations often observed in NMR spectra of IDPs call for resolution- and sensitivity-optimized pulse schemes. We present here a set of amide proton-detected 3D BEST-TROSY correlation experiments that yield the required sensitivity and spectral resolution for time-efficient sequential resonance assignment of large IDPs. In addition, we introduce two proline-edited 2D experiments that allow unambiguous identification of residues adjacent to proline that is one of the most abundant amino acids in IDPs. The performance of these experiments, and the advantages of BEST-TROSY pulse schemes are discussed and illustrated for two IDPs of similar length (~270 residues) but with different conformational sampling properties.
authors: Institut de Biologie Structurale, Université Grenoble 1, 41 Rue Jules Horowitz, 38027 Grenoble Cedex 1, France.
authors: Zsofia
authors: Z
authors: Solyom
authors: Melanie
authors: M
authors: Schwarten
authors: Leonhard
authors: L
authors: Geist
authors: Robert
authors: R
authors: Konrat
authors: Dieter
authors: D
authors: Willbold
authors: Bernhard
authors: B
authors: Brutscher
mesh_terms: D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
chemical_list: D011506:Proteins