title: Improved protocol for recovery of bacterial DNA from water filters: Sonication and backflushing of commercial syringe filters.
abstract: Extraction of bacterial DNA from water filters is frequently used in aquatic microbial ecology, e.g., for sequencing-based diversity studies. Here, we describe an improved size-class filtration and bacterial DNA extraction protocol using commercial "syringe filters", involving sonication- and backflushing-steps and DNA-binding magnetic beads. Importantly, the sonication-step improved DNA recovery up to 10-fold. The DNA extraction-turnaround time is 4h, with a hands-on time of about 2h.
authors: Department of Biology, University of Konstanz,Konstanz, Germany.
authors: Anna Isabella
authors: AI
authors: Kesberg
authors: David
authors: D
authors: Schleheck
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification*; D005374:Filtration; D008280:Magnetics; D008851:Micropore Filters; D008863:Microspheres; D013010:Sonication; D013048:Specimen Handling / Q000379:methods*; D013594:Syringes; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial