title: Modulation of expression of p16 and her2 in rat breast tissues of mammary hyperplasia model by external use of rupifang extract.
abstract: OBJECTIVE
  To observe the effect of Rupifang Extract in external use on expression of proto-oncogenes her2 and tumor suppression genes p16 in rat breast tissues of mammary hyperplasia model. To explore the mechanisms of Rupifang Extract in external use for preventing and treating mammary hyperplasia.
  
  
  METHODS
  Thirty virginal female Wistar rats were randomized into 5 groups, 6 in each, A: blank control group; B: model group; C: the low dose group of Rupifang; D: the middle dose group of Rupifang; and E: The high dose group of Rupifang. The mammary hyperplasia rat models were produced by injecting estradiol benzoate and progesterone and irritating by tail nipping. Drug intervention was also launched during the model formation. After 30 days, the expression of her2 and p16 in breast tissues of rats in each group were detected by the SP immunohistochemical method.
  
  
  RESULTS
  Compared with Blank control group, the expression of her2 in breast tissues in Model group was higher, and the expression of p16 was lower (P < 0.05 or P < 0.01). After intervention with Rupifang Extract, compared with Model group, the expression of her2 in breast tissues in Rupifang groups was lower, and the expression of p16 higher (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  The mechanisms of Rupifang Extract in external application for preventing and treating mammary hyperplasia may be reducing the expression of proto-oncogenes her2 and increasing the expression of tumor suppression genes p16.
authors: The School Outpatient Department, First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
authors: Guijuan
authors: G
authors: Zhang
authors: Dehui
authors: D
authors: Li
authors: Hui
authors: H
authors: Guo
authors: Yi
authors: Y
authors: Ma
authors: Rui
authors: R
authors: Liao
authors: Bizhu
authors: B
authors: Tan
authors: Suyi
authors: S
authors: Zhang
authors: Yubin
authors: Y
authors: Liu
authors: Min
authors: M
authors: Ma
authors: Xiaoting
authors: X
authors: Zeng
authors: Zhuolong
authors: Z
authors: Peng
authors: Si
authors: S
authors: Huang
authors: Yinghui
authors: Y
authors: Zheng
mesh_terms: D000818:Animals; D001941:Breast Diseases / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006965:Hyperplasia; D042361:Mammary Glands, Human / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004365:Drugs, Chinese Herbal; D018719:Erb-b2 Receptor Tyrosine Kinases