title: High throughput LC-MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine in human plasma.
abstract: A selective and sensitive high performance liquid chromatography-tandem mass spectrometry method has been developed and validated for simultaneous determination of zidovudine (ZDV), lamivudine (3TC) and nevirapine (NVP) in human plasma. After Solid phase extraction (SPE), analytes and ISTDs were run on Peerless Basic C18 column with an injection volume of 3μL and run time of 3.0min. An isocratic mobile phase of 0.1% formic acid in water:methanol (15:85, v/v) was used with positive mass spectrometric detection. The method was validated over a concentration range of 5-1500ng/mL for ZDV and 3TC and over the concentration range of 10-3000ng/mL for NVP. The intraday and interday precision and accuracy across four validation runs were ranged from 1.6 to 10.1% and 93.8 to 110.8% respectively.
authors: Department of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, India; Axis Clinicals Limited, 1-121/1, Miyapur, Hyderabad 500 049, India.
authors: Valluru Rajani
authors: VR
authors: Kumar
authors: B Phani Bhushana
authors: BP
authors: Reddy
authors: B Ravi
authors: BR
authors: Kumar
authors: K
authors: K
authors: Sreekanth
authors: Kilaru Naveen
authors: KN
authors: Babu
mesh_terms: D019380:Anti-HIV Agents / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D019259:Lamivudine / Q000097:blood*; D008297:Male; D019829:Nevirapine / Q000097:blood*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015215:Zidovudine / Q000097:blood*
chemical_list: D019380:Anti-HIV Agents; D019259:Lamivudine; D015215:Zidovudine; D019829:Nevirapine