title: Chemo-enzymatic saccharification and bioethanol fermentation of lipid-extracted residual biomass of the microalga, Dunaliella tertiolecta.
abstract: Chemo-enzymatic saccharification and bioethanol fermentation of the residual biomass of Dunaliella tertiolecta after lipid extraction for biodiesel production were investigated. HCl-catalyzed saccharification of the residual biomass at 121 °C for 15 min produced reducing sugars with a yield of 29.5% (w/w) based on the residual biomass dry weight. Various enzymes were evaluated for their ability to saccharify the residual biomass. Enzymatic saccharification using AMG 300 L produced 21.0 mg/mL of reducing sugar with a yield of 42.0% (w/w) based on the residual biomass at pH 5.5 and 55 °C. Bioethanol was produced from the enzymatic saccharification products without additional pretreatment by Saccharomyces cerevisiae with yields of 0.14 g ethanol/g residual biomass and 0.44 g ethanol/g glucose produced from the residual biomass. The waste residual biomass generated during microalgal biodiesel production could be used for the production of bioethanol to improve the economic feasibility of microalgal biorefinery.
authors: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors: Ok Kyung
authors: OK
authors: Lee
authors: A Leum
authors: AL
authors: Kim
authors: Dong Ho
authors: DH
authors: Seong
authors: Choul Gyun
authors: CG
authors: Lee
authors: Yeon Tae
authors: YT
authors: Jung
authors: Jin Won
authors: JW
authors: Lee
authors: Eun Yeol
authors: EY
authors: Lee
mesh_terms: D056804:Biofuels; D018533:Biomass; D002241:Carbohydrates / Q000096:biosynthesis*; D002480:Cellulase / Q000378:metabolism; D000460:Chlorophyta / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000302:isolation & purification; D009097:Multienzyme Complexes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013696:Temperature
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D008055:Lipids; D009097:Multienzyme Complexes; C578134:Viscozyme L, Aspergillus; D000431:Ethanol; D005087:Glucan 1,4-alpha-Glucosidase; D002480:Cellulase