title: Stabilization of Cox1p intermediates by the Cox14p-Coa3p complex.
abstract: Cox14p and Coa3p have been shown to regulate translation of the mitochondrial COX1 mRNA and to be required for assembly of cytochrome oxidase. We present evidence that Cox14p and Coa3p stabilize previously identified Cox1p intermediates and that in the absence of either protein, Cox1p aggregates with itself and other mitochondrial gene products, including cytochrome b, Var1p and Cox2p. Our evidence suggests that Cox1p assembly intermediates are in close proximity to other mitochondrially translated proteins and that an important function of Cox14p and Coa3p is to prevent Cox1 from entering into unproductive aggregation pathways.
authors: Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
authors: Gavin P
authors: GP
authors: McStay
authors: Chen Hsien
authors: CH
authors: Su
authors: Alexander
authors: A
authors: Tzagoloff
mesh_terms: D015153:Blotting, Western; D045303:Cytochromes b / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C094357:COX14 protein, S cerevisiae; C553333:Coa3 protein, S cerevisiae; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C026383:VAR1 protein, S cerevisiae; D045303:Cytochromes b; C117108:cytochrome C oxidase subunit II; C526453:Cox1 protein, S cerevisiae; D003576:Electron Transport Complex IV