title: Effects of salinomycin on human bone marrow-derived mesenchymal stem cells in vitro.
abstract: Various hypotheses on the origin of cancer stem cells (CSCs) exist, including that CSCs develop from transformed human bone marrow mesenchymal stem cells (hBMSC). Since the polyether antibiotic salinomycin selectively kills CSCs, the present study aims to elucidate the effects of salinomycin on normal hBMSC. The immunophenotype of hBMSC after salinomycin exposure was observed by flow cytometry. The multi-differentiation capacity of hBMSC was evaluated by Oil Red O and van Kossa staining. Cytotoxic effects of salinomycin were monitored by the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay. Furthermore, spheroid formation and migration capacity were assessed. There were no differences in the immunophenotype and multi-differentiation capacity of hBMSC induced by salinomycin treatment. Cytotoxic effects were observed at concentrations of 30 μM and above. Neither the migration capability nor the ability to form spheroids was affected. Essential functional properties of hBMSC were unaffected by salinomycin. However, dose-dependent cytotoxicity effects could be observed. Overall, low dose salinomycin showed no negative effects on hBMSC. Since mesenchymal stem cells from various sources respond differently, further in vitro studies are needed to clarify the effect of salinomycin on tissue-specific stem cells.
authors: Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Josef-Schneider Str. 11, 97080 Würzburg, Germany.
authors: A
authors: A
authors: Scherzed
authors: S
authors: S
authors: Hackenberg
authors: K
authors: K
authors: Froelich
authors: K
authors: K
authors: Rak
authors: A
authors: A
authors: Technau
authors: A
authors: A
authors: Radeloff
authors: U
authors: U
authors: Nöth
authors: C
authors: C
authors: Koehler
authors: R
authors: R
authors: Hagen
authors: N
authors: N
authors: Kleinsasser
mesh_terms: D001854:Bone Marrow Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D011714:Pyrans / Q000633:toxicity*; D000095662:Polyether Polyketides
chemical_list: D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides