title: Detection of DENV-4 genotype I from mosquitoes collected in the city of Manaus, Brazil.
abstract: BACKGROUND
  Dengue epidemics have been reported in Brazil since 1981. In Manaus, a large city in the Amazon region, dengue is endemic with all four-virus serotypes (DENV-1, -2, -3, and -4) simultaneously causing human disease. In 2008, during a surveillance of dengue virus in mosquitoes in the district of Tancredo Neves in Manaus, 260 mosquitoes of Aedes genus were captured, identified and grouped into pools of 10 mosquitoes.
  
  
  RESULTS
  RNA extracts of mosquito pools were tested by a RT-Hemi-Nested-PCR for detection of flaviviruses. One amplicon of 222 bp, compatible with dengue virus serotype 4, was obtained from a pool of Aedes aegypti. The nucleotide sequence of the amplicon indicated that the mosquitoes were infected with DENV-4 of genotype I. This virus of Asian origin has been described in Manaus in 2008 infecting acute febrile illness patients.
  
  
  CONCLUSIONS
  This is the first report of dengue virus serotype 4 genotype I infecting Aedes aegypti in the Americas.
authors: Fundação Osvaldo Cruz, Av Teresina, 476 - Adrianópolis, Manaus, AM, Brazil. marioluisgf@yahoo.com.br
authors: Mario Luis Garcia de
authors: ML
authors: Figueiredo
authors: Helda L
authors: HL
authors: Alfonso
authors: Alberto Anastacio
authors: AA
authors: Amarilla
authors: Luiz Tadeu Moraes
authors: LT
authors: Figueiredo
authors: Victor Hugo
authors: VH
authors: Aquino
authors: Cristóvão Alves da
authors: CA
authors: Costa
authors: Sergio Luiz Bessa
authors: SL
authors: Luz
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral