title: Marker-free dual-axis tilt series alignment.
abstract: Dual-axis tilt series in electron tomography are collected by successively tilting the object about two approximately orthogonal tilt axes. Here we report on the extension of marker-free image registration based on cross-correlation techniques to dual-axis tilt series. A simultaneous geometry refinement yields accurate parameters for the computation of the final reconstruction. Both, image registration and 3D-reconstruction operate on the combined data from the paired single axis series rather than computing individual single axis tomograms followed by a separate combination step. We show that with simultaneous registration and reconstruction of dual-axis tilt series, tomograms with higher resolution are obtained than by merging separately computed tomograms.
authors: Florida State University, Institute of Molecular Biophysics, Tallahassee, FL 32306, USA. winkler@sb.fsu.edu
authors: Hanspeter
authors: H
authors: Winkler
authors: Kenneth A
authors: KA
authors: Taylor
mesh_terms: D055032:Electron Microscope Tomography / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008968:Molecular Conformation; D012984:Software