title: Global approaches for telling time: omics and the Arabidopsis circadian clock.
abstract: The circadian clock is an endogenous timer that anticipates and synchronizes biological processes to the environment. Traditional genetic approaches identified the underlying principles and genetic components, but new discoveries have been greatly impeded by the embedded redundancies that confer necessary robustness to the clock architecture. To overcome this, global (omic) techniques have provided a new depth of information about the Arabidopsis clock. Our understanding of the factors, regulation, and mechanistic connectivity between clock genes and with output processes has substantially broadened through genomic (cDNA libraries, yeast one-hybrid, protein binding microarrays, and ChIP-seq), transcriptomic (microarrays, RNA-seq), proteomic (mass spectrometry and chemical libraries), and metabolomic (mass spectrometry) approaches. This evolution in research will undoubtedly enhance our understanding of how the circadian clock optimizes growth and fitness.
authors: Section of Cell and Developmental Biology and Center for Chronobiology, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, United States. bchow@ucsd.edu
authors: Brenda Y
authors: BY
authors: Chow
authors: Steve A
authors: SA
authors: Kay
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D057906:Circadian Clocks / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D020411:Oligonucleotide Array Sequence Analysis; D040081:Protein Array Analysis; D025941:Protein Interaction Mapping; D040901:Proteomics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors