title: [3-nitrotyrosine determination as nitrosative stress marker and health attitudes of medical students considering exposure to environmental tobacco smoke].
abstract: Negative attitudes in health such as cigarette smoking and imbalanced diet play important role in pathogenesis of various diseases. Cigarette smoking constitutes one of the main sources of exposure to cancerogenic and procancerogenic xenobiotics among adults as well as among young people. Many studies have proven that cigarettes smokers more frequently follow less varied diet in comparison to non-smokers. Despite increasing knowledge of Poles regarding harmful effects of cigarettes smoking and numerous antinicotine campaigns, still high number of women and men smoke and the smoking percentage among young people remains high and has not decreased in the recent years. The ongoing research shows that free radicals -the man cause of exposure to oxidative stress- play the seminal role in pathogenesis of civilisation diseases and physiological cell aging processes. Reactive oxygen and nitrogen species present in cigarette smoke due to induced toxic compounds formation, are closely connected with observed increased risk of cancer, Chronic Obstructive Pulmonary Disease (COPD) and arteriosclerosis incidents. Malondialdehyde is one of the most studied product of lipid peroxidation and biomarker of oxidative stress. However, 3-nitrotyrosine is one of the most promising biomarkers regarding changes caused by oxidative stress in living organisms. The presence of 3-nitrotyrosine was observed in many diseases such as coronary artery disease, cancer and diabetes. The aim of the study was the evaluation of free radical processes increase related to tobacco smoke exposure and chosen diet habits by determination of 3-nitrotyrosine in plasma samples collected from the group of medicine students. In our investigation we used an author's questionnaire which served to estimate the exposure to tobacco smoke among medicine students. It took also into account the knowledge of the exposure to other xenobiotics and unhealthy habits/behaviours. The investigated group included 150 students of 1-st and 2-nd year of study at Faculty of Medicine and Dentistry Division in Zabrze, Silesian Medical University. 120 students provided blood samples for further analysis. In the study group 52 students were active smokers and the control group consisted of 68 non-smokers. 3-nitrotyrosine was determined with the use of ELISA technique. The mean concentration of 3-nitrotyrosine was higher in the group of smoking students in comparison to the control group. Diet habits also influenced the concentration of 3-nitrotyrosine. Eating food products of possibly high acrylamide level and drinking alcohol led in particular to observed increased concentrations of 3-nitrotyrosine in both students groups. The correlation between the food habits and the severity of nitrosative stress was also found.
authors: Katedra i Zakład Chemii, Wydział Lekarski z Oddziałem Lekarsko-Dentystycznym w Zabrzu, Slaski Uniwersytet Medyczny. mszumska@vp.pl
authors: Magdalena
authors: M
authors: Szumska
authors: Tomasz
authors: T
authors: Wielkoszyński
authors: Krystyna
authors: K
authors: Tyrpień
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000097:blood; D015415:Biomarkers / Q000097:blood; D004781:Environmental Exposure / Q000032:analysis / Q000706:statistics & numerical data; D004784:Environmental Monitoring; D005247:Feeding Behavior; D005260:Female; D007722:Health Knowledge, Attitudes, Practice; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D026361:Reactive Nitrogen Species / Q000032:analysis; D017382:Reactive Oxygen Species / Q000032:analysis; D012016:Reference Values; D012907:Smoking / Q000097:blood*; D013337:Students, Medical / Q000706:statistics & numerical data*; D011795:Surveys and Questionnaires; D014028:Tobacco Smoke Pollution / Q000032:analysis / Q000706:statistics & numerical data*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood; D055815:Young Adult
chemical_list: D015415:Biomarkers; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D014028:Tobacco Smoke Pollution; C002744:3-nitrotyrosine; D014443:Tyrosine