title: Treatment of MPS I mice with microencapsulated cells overexpressing IDUA: effect of the prednisolone administration.
abstract: Cell encapsulation, although a promising strategy to deliver therapeutic products, is hampered by immune response against biomaterials. The aim of this article is to assess the effect of prednisolone on enzyme release by microencapsulated cells implanted in vivo. Recombinant cells encapsulated were implanted in the peritoneum of wild-type mice and mucopolysaccharidosis (MPS) I mice, with or without prednisolone. Later, microcapsules were recovered for histological and enzyme analysis. Blood was collected from MPS I mice. All animals receiving prednisolone had a smaller inflammatory infiltrate. In vitro, prednisolone increased the amount of enzyme released from the recovered capsules, but this was not accompanied by an increase in the amount of circulating enzyme in vivo after 15 days. However, in 7 days, prednisolone significantly increased the amount of enzyme detected in the serum. Although prednisolone improved enzyme release in vitro and in vivo after 7 days, it was unable to maintain this effect for a longer period.
authors: Post-Graduation Program on Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Valeska Lizzi
authors: VL
authors: Lagranha
authors: Talita Giacomet
authors: TG
authors: de Carvalho
authors: Roberto
authors: R
authors: Giugliani
authors: Ursula
authors: U
authors: Matte
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D002460:Cell Line; D018914:Cells, Immobilized / Q000637:transplantation; D006224:Cricetinae; D056947:Enzyme Replacement Therapy / Q000379:methods*; D005260:Female; D006801:Humans; D007068:Iduronidase / Q000096:biosynthesis / Q000235:genetics / Q000493:pharmacokinetics / Q000627:therapeutic use; D051379:Mice; D008059:Mucopolysaccharidosis I / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D011239:Prednisolone / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D011239:Prednisolone; D007068:Iduronidase