title: An anti-tumor protein produced by Trichinella spiralis induces apoptosis in human hepatoma H7402 cells.
abstract: Trichinella spiralis infection confers effective resistance to tumor cell expansion. In this study, a T7 phage cDNA display library was constructed to express genes encoded by T. spiralis. Organic phase multi-cell screening was used to sort through candidate proteins in a transfected human chronic myeloid leukemia cell line (K562) and a human hepatoma cell line (H7402) using the display library. The protein encoded by the A200711 gene was identified and analyzed using protein analysis software. To test the antitumor effects of A200711, variations in cell proliferation and apoptosis were monitored after recombinant pEGFP-N1-A200711 was transfected into H7402 cells. The results show that the expressed target gene successfully induced apoptosis in H7402 cells as measured by Hoechst-PI staining, MTT assay (p<0.05). This study warrants further investigation into the therapeutic use of A200711 for anti-hepatocellular carcinomas.
authors: Key Lab for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
authors: X L
authors: XL
authors: Wang
authors: M Y
authors: MY
authors: Liu
authors: S M
authors: SM
authors: Sun
authors: X L
authors: XL
authors: Liu
authors: L
authors: L
authors: Yu
authors: X R
authors: XR
authors: Wang
authors: L X
authors: LX
authors: Chu
authors: B
authors: B
authors: Rosenthal
authors: H N
authors: HN
authors: Shi
authors: P
authors: P
authors: Boireau
authors: F
authors: F
authors: Wang
authors: Y
authors: Y
authors: Zhao
authors: X P
authors: XP
authors: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D061505:Cell Surface Display Techniques; D019295:Computational Biology; D015723:Gene Library; D049452:Green Fluorescent Proteins; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D011993:Recombinant Fusion Proteins; D017160:Trichinella spiralis / Q000235:genetics* / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins