title: Erythrocyte membrane-cloaked polymeric nanoparticles for controlled drug loading and release.
abstract: OBJECTIVE
  Polymeric nanoparticles (NPs) cloaked by red blood cell membrane (RBCm) confer the combined advantage of both long circulation lifetime and controlled drug release. The authors carried out studies to gain a better understanding of the drug loading, drug-release kinetics and cell-based efficacy of RBCm-cloaked NPs.
  
  
  METHODS
  Two strategies for loading doxorubicin into the RBCm-cloaked NPs were compared: physical encapsulation and chemical conjugation. In vitro efficacy was examined using the acute myeloid leukemia cell line, Kasumi-1.
  
  
  RESULTS
  It was found that the chemical conjugation strategy resulted in a more sustained drug release profile, and that the RBCm cloak provided a barrier, retarding the outward diffusion of encapsulated drug molecules. It was also demonstrated that RBCm-cloaked NPs exhibit higher toxicity in comparison with free doxorubicin.
  
  
  CONCLUSIONS
  These results indicate that the RBCm-cloaked NPs hold great promise to become a valuable drug-delivery platform for the treatment of various diseases such as blood cancers.
authors: Department of Nano Engineering, University of California, San Diego, 9500 Gilman Drive, MC-0448, La Jolla, CA 92093-0448, USA.
authors: Santosh
authors: S
authors: Aryal
authors: Che-Ming J
authors: CM
authors: Hu
authors: Ronnie H
authors: RH
authors: Fang
authors: Diana
authors: D
authors: Dehaini
authors: Cody
authors: C
authors: Carpenter
authors: Dong-Er
authors: DE
authors: Zhang
authors: Liangfang
authors: L
authors: Zhang
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003692:Delayed-Action Preparations / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000473:pathology; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D011108:Polymers; D004317:Doxorubicin