title: Hepatic growth hormone resistance after acute injury.
abstract: Severe injury and infection are often followed by accelerated protein catabolism and acute insulin resistance. This results in several effects that complicate and prolong recovery, including weakness, immobility, impaired wound healing, and organ dysfunction. Recent studies have demonstrated the development of GH resistance during severe inflammation, providing a potential mechanism for the protein loss that follows injury and infection. To understand this GH resistance, we recently developed a murine model of acute injury. Mice were subjected to soft-tissue injury, alone or combined with hemorrhage, and injected iv with GH 30, 60, or 90 minutes later. Hepatic GH signaling was measured via Western analysis. GH-induced signal transducer and activator of transcription 5 phosphorylation was decreased immediately after completion of the trauma procedure, and at 30 and 60 minutes, but further decreased by 90 minutes after trauma. Combined trauma and hemorrhage resulted in severely decreased GH-induced signal transducer and activator of transcription 5 phosphorylation compared with trauma alone, and this was true at all time points studied. Western analysis revealed an apparent decrease in the molecular weight of the hepatic GH receptor (GHR) after trauma and hemorrhage, but not trauma alone. Additional studies determined that the hemorrhage-induced decrease in receptor size was not due to changes in GHR N-linked glycosylation. These results suggest that GH sensitivity is rapidly impaired after acute injury and that trauma combined with hemorrhage results in a more severe form of GH resistance resulting from alteration or inactivation of hepatic GHR.
authors: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294-0019, USA.
authors: Ryan M
authors: RM
authors: Corrick
authors: Li
authors: L
authors: Li
authors: Stuart J
authors: SJ
authors: Frank
authors: Joseph L
authors: JL
authors: Messina
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D013006:Growth Hormone / Q000008:administration & dosage / Q000378:metabolism*; D006470:Hemorrhage / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011986:Receptors, Somatotropin / Q000378:metabolism; D050799:STAT5 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D017695:Soft Tissue Injuries / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002352:Carrier Proteins; D011986:Receptors, Somatotropin; D050799:STAT5 Transcription Factor; D013006:Growth Hormone; C051815:somatotropin-binding protein