title: Approach for plasmonic based DNA sensing: amplification of the wavelength shift and simultaneous detection of the plasmon modes of gold nanostructures.
abstract: In this article, the detection of DNA hybridization taking advantage of the plasmonic properties of gold nanostructures is described. The approach is based on the amplification of the wavelength shift of a multilayered localized surface plasmon resonance (LSPR) sensor interface upon hybridization with gold nanorods and nanostars-labeled DNA. The amplification results in a significant decrease of the limit of detection from ≈40 nM as observed for unlabeled DNA to 0.2 nM for labeled DNA molecules. Furthermore, the plasmonic band, characteristic of the labeled DNA, is different from that of the LSPR interface. Indeed, next to the plasmon band at around 550 nm, being in resonance with the plasmon band of the LSPR interface, additional plasmonic peaks at 439 nm for gold nanostar-labeled DNA and 797 nm for gold nanorod-labeled DNA are observed, which were used as plasmonic signatures for successful hybridization.
authors: Laboratoire de Réactivité de Surfaces, UMR CNRS 7197, Université Pierre & Marie Curie-Paris VI, Ivry-sur-Seine, France. jolanda.spadavecchia@upmc.fr
authors: Jolanda
authors: J
authors: Spadavecchia
authors: Alexandre
authors: A
authors: Barras
authors: Joel
authors: J
authors: Lyskawa
authors: Patrice
authors: P
authors: Woisel
authors: William
authors: W
authors: Laure
authors: Claire-Marie
authors: CM
authors: Pradier
authors: Rabah
authors: R
authors: Boukherroub
authors: Sabine
authors: S
authors: Szunerits
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D015342:DNA Probes; D006046:Gold; D004247:DNA