title: Characterization of flocculating agent from the self-flocculating microalga Scenedesmus obliquus AS-6-1 for efficient biomass harvest.
abstract: In the present work, the extracellular biopolymers from the self-flocculating microalga Scenedesmus obliquus AS-6-1 were studied. It was revealed that the self-flocculation of the microalgal cells was mediated by cell wall-associated polysaccharides with a molecular weight of 127.9 kDa. Sugar compositions analysis indicated that the monomers consist of glucose, mannose, galatose, rhamnose and fructose with the molar ratio of 8:5:3:2:1. Addition of 0.6 mg/L purified flocculating agent resulted in the fast flocculation of freely suspended cells of S. obliquus and Chlorella vulgaris. The flocculating activity is stable between pH 6 and 8 and at 20-60°C.
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Suo-Lian
authors: SL
authors: Guo
authors: Xin-Qing
authors: XQ
authors: Zhao
authors: Chun
authors: C
authors: Wan
authors: Zih-You
authors: ZY
authors: Huang
authors: Yu-Liang
authors: YL
authors: Yang
authors: Md Asraful
authors: MA
authors: Alam
authors: Shih-Hsin
authors: SH
authors: Ho
authors: Feng-Wu
authors: FW
authors: Bai
authors: Jo-Shu
authors: JS
authors: Chang
mesh_terms: D018533:Biomass; D001704:Biopolymers / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry; D005427:Flocculation / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D044444:Scenedesmus / Q000737:chemistry*; D013624:Taiwan; D013696:Temperature
chemical_list: D001704:Biopolymers; D011134:Polysaccharides
keywords: Cell flocculation; Flocculating agent; Microalgae harvesting; Polysaccharide; Scenedesmus obliquus