title: Preparation of cell lines for single-cell analysis of transcriptional activation dynamics.
abstract: Imaging molecularly defined regions of chromatin in single living cells during transcriptional activation has the potential to provide new insight into gene regulatory mechanisms. Here, we describe a method for isolating cell lines with multi-copy arrays of reporter transgenes, which can be used for real-time high-resolution imaging of transcriptional activation dynamics in single cells.
authors: Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA, USA.
authors: Ilona U
authors: IU
authors: Rafalska-Metcalf
authors: Susan M
authors: SM
authors: Janicki
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D018929:Cell Culture Techniques; D002460:Cell Line; D017930:Genes, Reporter; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods*; D015533:Transcriptional Activation; D014162:Transfection; D019076:Transgenes
chemical_list: D002130:Calcium Phosphates; C020243:calcium phosphate