title: M14K and M38K malignant pleural mesothelioma cell lines preserve the same claudin-based phenotype in vivo.
abstract: BACKGROUND
  Malignant pleural mesothelioma (MPM) is an aggressive neoplasm with few treatment options. Reliable tumour cell lines for mesothelioma research are rare. Claudins seem to be attractive targets for cancer therapy.
  
  
  OBJECTIVE
  To establish a claudin-based MPM phenotype and to verify whether it is preserved in vitro and in vivo.
  
  
  METHODS
  Claudin-3 and -4 expression was examined by immunohistochemistry and immunoblotting in MPM (n=15) and lung adenocarcinoma (n=5) specimens. Claudin-3, -4 and -15 expression was also assessed in MPM versus adenocarcinoma cell lines and in MPM versus adenocarcinoma-derived tumour xenografts mouse models.
  
  
  RESULTS
  A defined MPM phenotype was established: M14K and M38K cell lines highly express Claudin-15 and calretinin but not claudin-3 nor claudin-4. Similar results were obtained in xenograft mouse models.
  
  
  CONCLUSIONS
  M14K and M38K cell lines, whether in vitro or in an animal model are representative models and appropriate in exploring new therapeutic strategies in MPM that may target claudins.
authors: Department of Biology, Kouba High School, Algiers, Algeria.
authors: Siham
authors: S
authors: Chaouche-Mazouni
authors: Marie-Christine
authors: MC
authors: Copin
authors: Philippe
authors: P
authors: Lassalle
authors: Nemcha
authors: N
authors: Lebaili
authors: Alexis
authors: A
authors: Cortot
authors: Arnaud
authors: A
authors: Scherpereel
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D064032:Calbindin 2; D045744:Cell Line, Tumor; D057167:Claudins / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008654:Mesothelioma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D000086002:Mesothelioma, Malignant; D051379:Mice; D016513:Mice, SCID; D058990:Molecular Targeted Therapy; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D010997:Pleural Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: C576764:CALB2 protein, human; C576511:Calb2 protein, mouse; D064032:Calbindin 2; D057167:Claudins; D064030:S100 Calcium Binding Protein G