title: Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity.
abstract: The purpose of this study was to investigate the neuroprotective effect of a water-soluble formulation of coenzyme Q10 (WS-CoQ10) in PC12 cells exposed to cisplatin, a chemotherapeutic agent with a dose-limiting factor due to neurotoxicity. In the cytokinesis-block micronucleus cytome assay (CBMN Cyt), WS-CoQ10 (at concentrations of 0.1, 0.5 and 1.0μgmL(-1)) protected PC12 cells from cisplatin-induced DNA damage (0.1μgmL(-1)), reducing the frequency of micronuclei (MNi) and nuclear buds (NBUDs). WS-CoQ10 did not alter the mRNA expression levels of Tp53 (at a concentration of 1.0μgmL(-1)) and exhibited neuroprotective activity by stimulating cisplatin-inhibited neurite outgrowth in nerve growth factor (NGF)-differentiated PC12 cells (at a concentration of 0.1μgmL(-1)). In conclusion, WS-CoQ10 protected the PC12 cells from cisplatin-induced DNA damage and neurotoxicity. Moreover, the neuroprotective effects of WS-CoQ10 suggest a possible application in chemotherapeutic protocols.
authors: Department of Clinical, Toxicological and Bromatological Analyses, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil. carlasm@fcfrp.usp.br
authors: Carla
authors: C
authors: da Silva Machado
authors: Leonardo Meneghin
authors: LM
authors: Mendonça
authors: Vinicius de Paula
authors: VP
authors: Venancio
authors: Maria Lourdes Pires
authors: ML
authors: Bianchi
authors: Lusânia Maria Greggi
authors: LM
authors: Antunes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002454:Cell Differentiation / Q000187:drug effects; D002945:Cisplatin / Q000633:toxicity*; D020552:Comet Assay; D016207:Cytokines / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology; D016716:PC12 Cells; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D014451:Ubiquinone; D020932:Nerve Growth Factor; C024989:coenzyme Q10; D005978:Glutathione; D002945:Cisplatin; D019803:Glutathione Disulfide