title: In vitro studies on α-glucosidase inhibition, antioxidant and free radical scavenging activities of Hedyotis biflora L.
abstract: Aim of this study was to evaluate the in vitro α-glucosidase inhibition and antioxidant activity of hexane, ethyl acetate and methanol extracts of Hedyotis biflora L. (Rubiaceae). In in vitro α-glucosidase inhibition and antioxidant activity, the methanol extract showed potent effect compared to hexane and ethyl acetate extracts. The methanol extract of H. biflora (HBMe) showed 50% α-glucosidase inhibition at the concentration of 480.20 ± 2.37 μg/ml. The total phenolic content of HBMe was 206.81 ± 1.11 mg of catechol equivalents/g extract. HBMe showed great scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC(50) 520.21 ± 1.02 μg/ml), hydroxyl (IC(50) 510.21 ± 1.51 μg/ml), nitric oxide (IC(50) 690.20 ± 2.13 μg/ml) and superoxide (IC(50) 510.31 ± 1.45 μg/ml) radicals, as well as high reducing power. HBMe also showed a strong suppressive effect on lipid peroxidation. Using the β-carotene method, the scavenging values of HBMe was significantly lower than BHT, and metal chelating ability of HBMe also showed a strong inhibition effect when compared to the reference standard. The active compound ursolic acid from HBMe was identified using various spectroscopical studies. The results obtained in this study clearly indicate that HBMe has a significant potential to use as a natural α-glucosidase inhibition, antioxidant agent.
authors: Department of Plant Biology and Biotechnology, Loyola College, Chennai 600 034, India.
authors: I V S
authors: IV
authors: Nimal Christhudas
authors: P
authors: P
authors: Praveen Kumar
authors: Christudas
authors: C
authors: Sunil
authors: S
authors: S
authors: Vajravijayan
authors: R
authors: R
authors: Lakshmi Sundaram
authors: S
authors: S
authors: Jenifer Siril
authors: P
authors: P
authors: Agastian
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors; D032064:Hedyotis / Q000737:chemistry*; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats
chemical_list: D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts