title: A rapid liquid chromatography tandem mass spectrometry-based method for measuring propranolol on dried blood spots.
abstract: Propranolol, a non-selective beta blocker drug, is used in young infants and newborns for treating several heart diseases; its pharmacokinetics has been extensively evaluated in adult patients using extrapolation to treat pediatric population. The purpose of the present study was to develop and validate a method to measure propranolol levels in dried blood spots. The analysis was performed by using liquid chromatography/tandem mass spectrometry operating in multiple reaction monitoring mode. The calibration curve in matrix was linear in the concentration range of 2.5-200 μg/L with correlation coefficient r=0.9996. Intra-day and inter-day precisions and biases were less than 8.0% (n=10) and 11.5% (n=10) respectively. The recoveries ranged from 94 to 100% and the matrix effect did not result in a severe signal suppression. Propranolol on dried blood spot showed a good stability at three different temperatures for one month. This paper describes a micromethod for measuring propranolol levels on dried blood spot, which determines a great advantage in neonates or young infants during pharmacokinetic studies because of less invasive sampling and small blood volume required.
authors: Newborn Screening, Biochemistry and Pharmacology Laboratories, Clinic of Pediatric Neurology, Meyer University Children's Hospital, Florence, Italy.
authors: Maria Luisa
authors: ML
authors: Della Bona
authors: Sabrina
authors: S
authors: Malvagia
authors: Fabio
authors: F
authors: Villanelli
authors: Elisa
authors: E
authors: Giocaliere
authors: Daniela
authors: D
authors: Ombrone
authors: Silvia
authors: S
authors: Funghini
authors: Luca
authors: L
authors: Filippi
authors: Giacomo
authors: G
authors: Cavallaro
authors: Paola
authors: P
authors: Bagnoli
authors: Renzo
authors: R
authors: Guerrini
authors: Giancarlo
authors: G
authors: la Marca
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011433:Propranolol / Q000097:blood*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000319:Adrenergic beta-Antagonists; D011433:Propranolol