title: Effect of 830-nm diode laser irradiation on human sperm motility.
abstract: Sperm motility is known as an effective parameter in male fertility, and it depends on energy consumption. Low-level laser irradiation could increase energy supply to the cell by producing adenosine triphosphate. The purpose of this study is to evaluate how the low-level laser irradiation affects the human sperm motility. Fresh human semen specimens of asthenospermic patients were divided into four equal portions and irradiated by 830-nm GaAlAs laser irradiation with varying doses as: 0 (control), 4, 6 and 10 J/cm(2). At the times of 0, 30, 45 and 60 min following irradiation, sperm motilities are assessed by means of computer-aided sperm analysis in all samples. Two additional tests [HOS and sperm chromatin dispersion (SCD) tests] were also performed on the control and high irradiated groups as well. Sperm motility of the control groups significantly decreased after 30, 45 and 60 min of irradiation, while those of irradiated groups remained constant or slightly increased by passing of time. Significant increases have been observed in doses of 4 and 6 J/cm(2) at the times of 60 and 45 min, respectively. SCD test also revealed a non-significant difference. Our results showed that irradiating human sperms with low-level 830-nm diode laser can improve their progressive motility depending on both laser density and post-exposure time.
authors: Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran, r_salman_yazdi@yahoo.com.
authors: Reza
authors: R
authors: Salman Yazdi
authors: Simin
authors: S
authors: Bakhshi
authors: Firooz
authors: F
authors: Jannat Alipoor
authors: Mohammad Reza
authors: MR
authors: Akhoond
authors: Soheila
authors: S
authors: Borhani
authors: Faramarz
authors: F
authors: Farrahi
authors: Mehdi
authors: M
authors: Lotfi Panah
authors: Mohammad Ali
authors: MA
authors: Sadighi Gilani
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000503:physiopathology / Q000532:radiotherapy; D053938:DNA Fragmentation / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D009997:Osmotic Pressure; D013081:Sperm Motility / Q000528:radiation effects*