title: Enzymatic but not compensated Jaffe methods reach the desirable specifications of NKDEP at normal levels of creatinine. Results of the French multicentric evaluation.
abstract: The French Society of Clinical Biochemistry conducted this study to compare the accuracy and performances of the best creatinine enzymatic assays and the compensated Jaffe methods from the same manufacturers. Creatinine was measured in 3 serum pools with creatinine levels of 35.9±0.9 μmol/L, 74.4±1.4 μmol/L, and 97.9±1.7 μmol/L (IDMS determination). The performances of the assays (total error that includes the contribution of bias and imprecision) were evaluated using Monte-Carlo simulations and compared against desirable NKDEP criteria. The enzymatic assays always fell within the desirable total Error of 7.6%. By contrast, this requirement was never obtained for the compensated Jaffe methods at the critical level of 74.4±1.4 μmol/L. Only the compensated Jaffe creatinine on Olympus analyzer reached this specification at 35.9±0.9 and 97.9±1.7 μmol/L levels. This study demonstrates that, despite substantial improvement regarding traceability to the IDMS reference method and precision, compensated Jaffe creatinine methods, by contrast to enzymatic ones, do not reach the desirable specifications of NKDEP at normal levels of creatinine.
authors: Biochimie, CHU Bichat, APHP, Paris, France.
authors: Anne
authors: A
authors: Boutten
authors: Anne-Sophie
authors: AS
authors: Bargnoux
authors: Marie-Christine
authors: MC
authors: Carlier
authors: Pierre
authors: P
authors: Delanaye
authors: Eric
authors: E
authors: Rozet
authors: Vincent
authors: V
authors: Delatour
authors: Etienne
authors: E
authors: Cavalier
authors: Anne-Marie
authors: AM
authors: Hanser
authors: Marc
authors: M
authors: Froissart
authors: Jean-Paul
authors: JP
authors: Cristol
authors: Laurence
authors: L
authors: Piéroni
mesh_terms: D003404:Creatinine / Q000097:blood* / Q000378:metabolism; D057075:Enzyme Assays; D005602:France; D006801:Humans; D007674:Kidney Diseases / Q000097:blood / Q000175:diagnosis*; D009313:National Health Programs; D012680:Sensitivity and Specificity
chemical_list: D003404:Creatinine