title: M tuberculosis in the adjuvant modulates time of appearance of CNS-specific effector T cells in the spleen through a polymorphic site of TLR2.
abstract: DC deliver information regulating trafficking of effector T cells along T-cell priming. However, the role of pathogen-derived motives in the regulation of movement of T cells has not been studied. We hereinafter report that amount of M tuberculosis in the adjuvant modulates relocation of PLP139-151 specific T cells. In the presence of a low dose of M tuberculosis in the adjuvant, T cells (detected by CDR3 BV-BJ spectratyping, the so-called "immunoscope") mostly reach the spleen by day 28 after immunization ("late relocation") in the SJL strain, whereas T cells reach the spleen by d 14 with a high dose of M tuberculosis ("early relocation"). The C57Bl/6 background confers a dominant "early relocation" phenotype to F1 (SJL×C57Bl/6) mice, allowing early relocation of T cells in the presence of low dose M tuberculosis. A single non-synonymous polymorphism of TLR2 is responsible for "early/late" relocation phenotype. Egress of T lymphocytes is regulated by TLR2 expressed on T cells. Thus, pathogens engaging TLR2 on T cells regulate directly T-cell trafficking, and polymorphisms of TLR2 condition T-cell trafficking upon a limiting concentration of ligand.
authors: Institute of General Pathology, Università Cattolica del S. Cuore, Rome, Italy.
authors: Chiara
authors: C
authors: Nicolò
authors: Gabriele
authors: G
authors: Di Sante
authors: Annabella
authors: A
authors: Procoli
authors: Giuseppe
authors: G
authors: Migliara
authors: Alessia
authors: A
authors: Piermattei
authors: Mariagrazia
authors: M
authors: Valentini
authors: Giovanni
authors: G
authors: Delogu
authors: Achille
authors: A
authors: Cittadini
authors: Gabriela
authors: G
authors: Constantin
authors: Francesco
authors: F
authors: Ria
mesh_terms: D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology; D001483:Base Sequence; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D002490:Central Nervous System / Q000276:immunology*; D005260:Female; D005838:Genotype; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide; D013154:Spleen / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D051195:Toll-Like Receptor 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D051195:Toll-Like Receptor 2