title: Effect of Pseudomonas aeruginosa elastase B on level and activity of immune proteins/peptides of Galleria mellonella hemolymph.
abstract: Abstract   Susceptibility of proteins and peptides present in immune hemolymph of Galleria mellonella Fabricius (Lepidoptera: Pyralidae) larvae to proteolytic degradation by purified elastase B of Pseudomonas aeruginosa was studied. Results showed that apoLp-III protein was gradually digested by elastase B in vitro. Additionally, polipeptides with molecular mass 6.5 and 4 kDa were degraded after treatment with the studied enzyme. The lack of these peptides and the decrease in anti-Escherichia coli activity could indicate that inducible antimicrobial peptides were digested by elastase B. On the contrary, no change in the lysosome activity level was observed in immune hemolymph incubated with elastase B. Thus, elastase B might contribute to the pathogenesis of P. aeruginosa.
authors: Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. mariola.andrejko@wp.pl
authors: Mariola
authors: M
authors: Andrejko
authors: Magdalena
authors: M
authors: Mizerska-Dudka
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000633:toxicity*; D004926:Escherichia coli / Q000276:immunology; D006458:Hemolymph / Q000378:metabolism; D056724:Immunity, Humoral; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000276:immunology; D008666:Metalloendopeptidases / Q000633:toxicity*; D009036:Moths / Q000254:growth & development / Q000276:immunology*; D059748:Proteolysis; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D001053:Apolipoproteins; D001426:Bacterial Proteins; D019476:Insect Proteins; C042470:apolipophorin III; D008666:Metalloendopeptidases; C043859:pseudolysin, Pseudomonas aeruginosa