title: Antigenic variation of H1N1, H1N2 and H3N2 swine influenza viruses in Japan and Vietnam.
abstract: The antigenicity of the influenza A virus hemagglutinin is responsible for vaccine efficacy in protecting pigs against swine influenza virus (SIV) infection. However, the antigenicity of SIV strains currently circulating in Japan and Vietnam has not been well characterized. We examined the antigenicity of classical H1 SIVs, pandemic A(H1N1)2009 (A(H1N1)pdm09) viruses, and seasonal human-lineage SIVs isolated in Japan and Vietnam. A hemagglutination inhibition (HI) assay was used to determine antigenic differences that differentiate the recent Japanese H1N2 and H3N2 SIVs from the H1N1 and H3N2 domestic vaccine strains. Minor antigenic variation between pig A(H1N1)pdm09 viruses was evident by HI assay using 13 mAbs raised against homologous virus. A Vietnamese H1N2 SIV, whose H1 gene originated from a human strain in the mid-2000s, reacted poorly with post-infection ferret serum against human vaccine strains from 2000-2010. These results provide useful information for selection of optimal strains for SIV vaccine production.
authors: Thailand-Japan Zoonotic Diseases Collaboration Center, Kasetklang, Chatuchak, Bangkok, 10900, Thailand.
authors: Nobuhiro
authors: N
authors: Takemae
authors: Tung
authors: T
authors: Nguyen
authors: Long Thanh
authors: LT
authors: Ngo
authors: Yasuaki
authors: Y
authors: Hiromoto
authors: Yuko
authors: Y
authors: Uchida
authors: Vu Phong
authors: VP
authors: Pham
authors: Tsutomu
authors: T
authors: Kageyama
authors: Shizuko
authors: S
authors: Kasuo
authors: Shinichi
authors: S
authors: Shimada
authors: Yasutaka
authors: Y
authors: Yamashita
authors: Kaoru
authors: K
authors: Goto
authors: Hideyuki
authors: H
authors: Kubo
authors: Vu Tri
authors: VT
authors: Le
authors: Hung
authors: H
authors: Van Vo
authors: Hoa Thi
authors: HT
authors: Do
authors: Dang Hoang
authors: DH
authors: Nguyen
authors: Tsuyoshi
authors: T
authors: Hayashi
authors: Aya
authors: A
authors: Matsuu
authors: Takehiko
authors: T
authors: Saito
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation / Q000235:genetics; D000956:Antigens, Viral / Q000235:genetics; D002642:Chick Embryo; D002645:Chickens; D004285:Dogs; D005289:Ferrets; D014644:Genetic Variation; D006168:Guinea Pigs; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D057221:Influenza A Virus, H1N2 Subtype / Q000235:genetics / Q000276:immunology*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000276:immunology*; D007564:Japan / Q000453:epidemiology; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012756:Sheep; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D014744:Vietnam / Q000453:epidemiology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral