title: Horizontal transmission of malignancy: in-vivo fusion of human lymphomas with hamster stroma produces tumors retaining human genes and lymphoid pathology.
abstract: We report the in-vivo fusion of two Hodgkin lymphomas with golden hamster cheek pouch cells, resulting in serially-transplanted (over 5-6 years) GW-532 and GW-584 heterosynkaryon tumor cells displaying both human and hamster DNA (by FISH), lymphoma-like morphology, aggressive metastasis, and retention of 7 human genes (CD74, CXCR4, CD19, CD20, CD71, CD79b, and VIM) out of 24 tested by PCR. The prevalence of B-cell restricted genes (CD19, CD20, and CD79b) suggests that this uniform population may be the clonal initiating (malignant) cells of Hodgkin lymphoma, despite their not showing translation to their respective proteins by immunohistochemical analysis. This is believed to be the first report of in-vivo cell-cell fusion of human lymphoma and rodent host cells, and may be a method to disclose genes regulating both organoid and metastasis signatures, suggesting that the horizontal transfer of tumor DNA to adjacent stromal cells may be implicated in tumor heterogeneity and progression. The B-cell gene signature of the hybrid xenografts suggests that Hodgkin lymphoma, or its initiating cells, is a B-cell malignancy.
authors: Center for Molecular Medicine and Immunology, Garden State Cancer Center, Morris Plains, NJ, USA. dmg.gscancer@att.net
authors: David M
authors: DM
authors: Goldenberg
authors: David V
authors: DV
authors: Gold
authors: Meiyu
authors: M
authors: Loo
authors: Donglin
authors: D
authors: Liu
authors: Chien-Hsing
authors: CH
authors: Chang
authors: Elaine S
authors: ES
authors: Jaffe
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002459:Cell Fusion / Q000379:methods; D045744:Cell Line, Tumor; D006224:Cricetinae; D004195:Disease Models, Animal; D018450:Disease Progression; D018014:Gene Transfer Techniques; D022761:Gene Transfer, Horizontal; D006689:Hodgkin Disease / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008647:Mesocricetus; D017154:Stromal Cells / Q000378:metabolism