title: A ratiometric fluorescent probe for hydrophobic proteins in aqueous solution based on aggregation-induced emission.
abstract: A novel fluorescent probe 1 is reported here with ratiometric response to hydrophobic proteins (casein) or proteins with hydrophobic pockets (BSA, HSA) through hydrophobic interaction. Probe 1 underwent deprotonation in aqueous solution at pH 7.4 and emitted blue fluorescence at 436 nm. Upon the addition of BSA, HSA or casein, the aggregation-induced emission fluorescence of 1 at 518 nm was turned on. The fluorescence intensity ratio, I518/I436 was linearly related to the concentrations of these proteins. The detection limits for BSA, HSA and casein based on IUPAC (CDL = 3Sb m(-1)) were 16.2 μg mL(-1), 10.5 μg mL(-1) and 5.7 μg mL(-1), respectively.
authors: Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors: Lu
authors: L
authors: Peng
authors: Ruirui
authors: R
authors: Wei
authors: Kai
authors: K
authors: Li
authors: Zhaojuan
authors: Z
authors: Zhou
authors: Panshu
authors: P
authors: Song
authors: Aijun
authors: A
authors: Tong
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007097:Imines / Q000138:chemical synthesis / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C584500:2,2',4,4'-tetrahydroxybenzophenone azine; D002364:Caseins; D005456:Fluorescent Dyes; D006834:Hydrazines; D007097:Imines; D012709:Serum Albumin; D012710:Serum Albumin, Bovine