title: Ezrin gene expression and protein production in the CD44(+) subpopulation of SCC-9 cells in a malignant oral cancer cell line in vitro.
abstract: OBJECTIVE
  The primary objective of this study was to examine ezrin in the CD44(+) subpopulation of SCC-9 cells in a malignant oral cancer cell line.
  
  
  METHODS
  The CD44(+) subpopulation of SCC-9 cells was sorted using CD44 fluorescence-activated cell sorting flow cytometry. Expressions of the ABCG2, CD133, Oct-4, BMI-1, an ezrin genes and proteins were detected by immunofluorescence and real-time polymerase chain reaction, and their differential expressions in the CD44(+) and CD44(-) cell populations and embryonic stem cells were compared. The location of the ezrin protein expressed in SCC-9 cells was determined using laser scanning confocal microscopy.
  
  
  RESULTS
  The expression of ezrin in the CD44(+) subpopulation was 38 times higher than in the CD44(-) subpopulation based on real-time polymerase chain reaction, and levels of ABCG2, CD133, Oct-4, and BMI-1 in the CD44(+) subpopulation were higher than those in the CD44(-) subpopulation (P < .05).
  
  
  CONCLUSIONS
  The overexpression of ezrin in the CD44(+) subpopulation of SCC-9 cells might affect the functions of CD44(+) tumor cells, which contribute to the metastatic behavior of malignant oral squamous cell carcinoma.
authors: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, China.
authors: Guomin
authors: G
authors: Wu
authors: Xiumei
authors: X
authors: Sun
authors: Hongyan
authors: H
authors: Yuan
authors: Min
authors: M
authors: Hu
mesh_terms: D000071916:AC133 Antigen; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D015703:Antigens, CD / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D018613:Microscopy, Confocal; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000737:chemistry; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010455:Peptides / Q000235:genetics; D063150:Polycomb Repressive Complex 1 / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000099149:Ezrin
chemical_list: D000071916:AC133 Antigen; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D015703:Antigens, CD; D003598:Cytoskeletal Proteins; D006023:Glycoproteins; D009363:Neoplasm Proteins; D050814:Octamer Transcription Factor-3; D010455:Peptides; D063150:Polycomb Repressive Complex 1; D000099149:Ezrin; C116614:ABCG2 protein, human; C069077:BMI1 protein, human; C000606023:PROM1 protein, human