title: Magnetic gold nanoparticles: synthesis, characterization and its application in the delivery of FITC into KG-1 cells.
abstract: In this article, we report a new method-a sonication method to disperse iron oxide nanoparticles into smaller nanoparticles and make gold ions absorb onto the surface or trapped in the micropores of the iron oxide nanoparticles using sonication action. By using quick reduction of ascorbic acid and post-HCI solution treatment, gold covered magnetic nanoparticles (mGNPs) with spherical morphology and uniform size were synthesized in a water solution. The size of the mGNPs was found to be 20-30 nm. Some ideal mGNPs possessed a core-shell structure. The mGNPs were non-cytotoxic and mGNP-fluorescein isothiocyanate (FITC) can enter KG-1 cells when driven by an external magnetic force, which was confirmed by confocal imaging. The confocal image also showed the FITC inside the KG-1 cells was near the nucleus. The fluorescein isothiocyanate (FITC) delivery efficiency is about 100% according to the flow cytometry results.
authors: Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta, T6G 2V4, Canada.
authors: Yuzhi
authors: Y
authors: Hao
authors: Steven
authors: S
authors: Song
authors: Xiaoyan
authors: X
authors: Yang
authors: James
authors: J
authors: Xing
authors: Jie
authors: J
authors: Chen
mesh_terms: D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
chemical_list: D006046:Gold; D016650:Fluorescein-5-isothiocyanate