title: Potato signal molecules that activate pectate lyase synthesis in Pectobacterium atrosepticum SCRI1043.
abstract: A new type of plant-derived signal molecules that activate extracellular pectate lyase activity in phytopathogenic bacterium Pectobacterium atrosepticum SCRI1043 was revealed. These compounds were characterized and partially purified by means of several approaches including RT-PCR analysis, luminescence bioassay and HPLC fractionation. They were smaller than 1 kDa, thermoresistant, nonproteinaceous, hydrophilic, and slightly negatively charged molecules. Using gene expression analysis and bacterial biosensor assay the mode of activity of revealed compounds was studied. The possibility of their action through quorum sensing- and KdgR-mediated pathways was analyzed.
authors: Kazan Research Center, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, PO Box 30, Kazan, 420111, Russia. tarasova@mail.knc.ru
authors: Nadezhda
authors: N
authors: Tarasova
authors: Vladimir
authors: V
authors: Gorshkov
authors: Olga
authors: O
authors: Petrova
authors: Yuri
authors: Y
authors: Gogolev
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D020869:Gene Expression Profiling; D044043:Pectobacterium / Q000187:drug effects* / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000096:biosynthesis*; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011133:Polysaccharide-Lyases; C023911:pectate lyase