title: Caveolin-1 up-regulates integrin α2,6-sialylation to promote integrin α5β1-dependent hepatocarcinoma cell adhesion.
abstract: The alterations of integrin glycosylation play a crucial role in tumor metastasis. Our previous studies indicated that caveolin-1 promoted the expression of the key α2,6-sialytransferase ST6Gal-I and fibronectin-mediated adhesion of mouse hepatocarcinoma cell. Herein, we investigated the role of α2,6-sialylated α5-integrin in the adhesion of mouse hepatocarcinoma H22 cell. We demonstrated that caveolin-1 up-regulated cell surface α2,6-linked sialic acid via stimulating ST6Gal-I transcription. Cell surface α2,6-sialylation was required for integrin α5β1-dependent cell adhesion to fibronectin, and an increase in α2,6-linked sialic acid on α5-subunit facilitated fibronectin-mediated focal adhesion kinase phosphorylations, suggesting that α2,6-sialylated α5-subunit promoted integrin α5β1-dependent cell adhesion.
authors: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116044, Liaoning Province, China.
authors: Shengjin
authors: S
authors: Yu
authors: Jianhui
authors: J
authors: Fan
authors: Linhua
authors: L
authors: Liu
authors: Lijun
authors: L
authors: Zhang
authors: Shujing
authors: S
authors: Wang
authors: Jianing
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051242:Caveolin 1 / Q000235:genetics* / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D005353:Fibronectins / Q000737:chemistry; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006031:Glycosylation; D039421:Integrin alpha2 / Q000235:genetics* / Q000378:metabolism; D039081:Integrin alpha5beta1 / Q000235:genetics* / Q000378:metabolism; D039503:Integrin alpha6 / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008156:Luciferases; D051379:Mice; D010766:Phosphorylation; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D051242:Caveolin 1; D005353:Fibronectins; D039421:Integrin alpha2; D039081:Integrin alpha5beta1; D039503:Integrin alpha6; D012794:Sialic Acids; D008156:Luciferases; D012799:Sialyltransferases; D051417:Focal Adhesion Kinase 1; C496051:Ptk2 protein, mouse; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase