title: Human perivascular stem cells show enhanced osteogenesis and vasculogenesis with Nel-like molecule I protein.
abstract: An ideal mesenchymal stem cell (MSC) source for bone tissue engineering has yet to be identified. Such an MSC population would be easily harvested in abundance, with minimal morbidity and with high purity. Our laboratories have identified perivascular stem cells (PSCs) as a candidate cell source. PSCs are readily isolatable through fluorescent-activated cell sorting from adipose tissue and have been previously shown to be indistinguishable from MSCs in the phenotype and differentiation potential. PSCs consist of two distinct cell populations: (1) pericytes (CD146+, CD34-, and CD45-), which surround capillaries and microvessels, and (2) adventitial cells (CD146-, CD34+, and CD45-), found within the tunica adventitia of large arteries and veins. We previously demonstrated the osteogenic potential of pericytes by examining pericytes derived from the human fetal pancreas, and illustrated their in vivo trophic and angiogenic effects. In the present study, we used an intramuscular ectopic bone model to develop the translational potential of our original findings using PSCs (as a combination of pericytes and adventitial cells) from human white adipose tissue. We evaluated human PSC (hPSC)-mediated bone formation and vascularization in vivo. We also examined the effects of hPSCs when combined with the novel craniosynostosis-associated protein, Nel-like molecule I (NELL-1). Implants consisting of the demineralized bone matrix putty combined with NELL-1 (3 μg/μL), hPSC (2.5×10(5) cells), or hPSC+NELL-1, were inserted in the bicep femoris of SCID mice. Bone growth was evaluated using microcomputed tomography, histology, and immunohistochemistry over 4 weeks. Results demonstrated the osteogenic potential of hPSCs and the additive effect of hPSC+NELL-1 on bone formation and vasculogenesis. Comparable osteogenesis was observed with NELL-1 as compared to the more commonly used bone morphogenetic protein-2. Next, hPSCs induced greater implant vascularization than the unsorted stromal vascular fraction from patient-matched samples. Finally, we observed an additive effect on implant vascularization with hPSC+NELL-1 by histomorphometry and immunohistochemistry, accompanied by in vitro elaboration of vasculogenic growth factors. These findings hold significant implications for the cell/protein combination therapy hPSC+NELL-1 in the development of strategies for vascularized bone regeneration.
authors: Dental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, UCLA, Los Angeles, California, USA.
authors: Asal
authors: A
authors: Askarinam
authors: Aaron W
authors: AW
authors: James
authors: Janette N
authors: JN
authors: Zara
authors: Raghav
authors: R
authors: Goyal
authors: Mirko
authors: M
authors: Corselli
authors: Angel
authors: A
authors: Pan
authors: Pei
authors: P
authors: Liang
authors: Le
authors: L
authors: Chang
authors: Todd
authors: T
authors: Rackohn
authors: David
authors: D
authors: Stoker
authors: Xinli
authors: X
authors: Zhang
authors: Kang
authors: K
authors: Ting
authors: Bruno
authors: B
authors: Péault
authors: Chia
authors: C
authors: Soo
mesh_terms: D000328:Adult; D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008297:Male; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D010012:Osteogenesis; D012756:Sheep; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D009419:Nerve Tissue Proteins