title: MALDI MS imaging analysis of apolipoprotein E and lysyl oxidase-like 1 in human lens capsules affected by pseudoexfoliation syndrome.
abstract: Pseudoexfoliation (PEX) syndrome is an age-related systemic disease of the extracellular matrix, characterized by the presence of amyloid-like fibrillar deposits on the anterior lens capsule. The pathological deposits (PEX material) can obstruct aqueous outflow leading to increased intraocular pressure that in turn can result in glaucoma. PEX syndrome is the most common risk factor for glaucoma. In our previous work, we reported a protocol for the analysis of human lens capsules by MALDI MS imaging. Here, we extend our previous work applying the developed protocol to the analysis of human lens capsules affected by PEX syndrome. We focus our investigation on known components of the PEX material, namely lysyl oxidase-like 1 (LOXL1) and apolipoprotein E (APOE). Our results show that LOXL1 is more abundant in the deposits in the iris region and, alternatively APOE is concentrated in the PEX material accumulated in the pupillary area of the anterior lens capsule. Furthermore, we identify potentially relevant post-translational modifications which may have an important role in promoting the cross-linking processes in PEX syndrome and stabilize aggregate structures within the proteinaceous PEX material.
  
  
  CONCLUSIONS
  This paper is about the identification and localization of apolipoprotein E and lysyl oxidase-like 1 in human lens capsules affected by PEX syndrome by MALDI MS imaging. With this study we expand the clinical application of MALDI MSI toward the use of non-sectioned tissue samples analyzed after in situ enzymatic digestion and advance the knowledge regarding a common pathology like PEX syndrome.
authors: Mawson Institute, University of South Australia, Mawson Lakes SA 5095, Australia. maurizio.ronci@unisa.edu.au
authors: Maurizio
authors: M
authors: Ronci
authors: Shiwani
authors: S
authors: Sharma
authors: Sarah
authors: S
authors: Martin
authors: Jamie E
authors: JE
authors: Craig
authors: Nicolas H
authors: NH
authors: Voelcker
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D001057:Apolipoproteins E / Q000032:analysis / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000379:methods*; D017889:Exfoliation Syndrome / Q000378:metabolism / Q000473:pathology; D005136:Eye Proteins / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000378:metabolism / Q000473:pathology; D008297:Male; D010944:Plants; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001057:Apolipoproteins E; D005136:Eye Proteins; D000594:Amino Acid Oxidoreductases; C445313:LOXL1 protein, human