title: The dominant roles of ICAM-1-encoding gene in DNA vaccination against Japanese encephalitis virus are the activation of dendritic cells and enhancement of cellular immunity.
abstract: We investigated the cellular immune responses elicited by a plasmid DNA vaccine encoding prM-E protein from the Japanese encephalitis (JE) virus (JEV) with or without various forms of intercellular adhesion molecule (ICAM)-1 gene to maximize the immune responses evoked by the JE DNA vaccine. We observed that co-immunization with the construct containing murine ICAM-1 gene (pICAM-1) resulted in a significant increase in the percentage of CD4(+)T cells, high level of JEV-specific cytotoxic T lymphocyte response, and high production of T helper 1 (Th1)-type cytokines in splenic T cells. Furthermore, the co-expression of ICAM-1 and DNA immunogens was found to be more effective in generating T cell-mediated immune responses than those induced by immunization with pJME in combination with pICAM-1. Our results suggested that ICAM-1 enhanced T cell receptor signaling and activated Th1 immune responses in the JEV model system by increasing the induction of CD4(+)Th1 cell subset and activating dendritic cells.
authors: Department of Infectious Diseases, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors: Yong-Zhen
authors: YZ
authors: Zhai
authors: Yan
authors: Y
authors: Zhou
authors: Li
authors: L
authors: Ma
authors: Guo-He
authors: GH
authors: Feng
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003713:Dendritic Cells / Q000276:immunology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology; D004672:Encephalitis, Japanese / Q000276:immunology / Q000517:prevention & control; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D022321:Japanese Encephalitis Vaccines / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D022321:Japanese Encephalitis Vaccines; D011948:Receptors, Antigen, T-Cell; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D018799:Intercellular Adhesion Molecule-1