title: Molecular characterization of an IL-1β gene from ayu, Plecoglossus altivelis.
abstract: IL-1β plays a crucial role as a prototypical proinflammatory cytokine in immune responses and has been shown to affect macrophage functions. However, the effects of putative IL-1β homologs on fish macrophages are still less known. Here, we cloned the full-length cDNA sequence of IL-1β (aIL-1β) gene from ayu, Plecoglossus altivelis. Phylogenetic analysis indicated that aIL-1β was closest to that of Atlantic salmon (Salmo salar). Real-time quantitative PCR (RT-qPCR) revealed that aIL-1β transcript was mainly expressed in spleen, head kidney and gill, and dramatically increased in various tissues after Listonella anguillarum infection. Subsequently, aIL-1β was prokaryotic expressed and purified to prepare anti-aIL-1β antibody. After L. anguillarum challenge, the aIL-1β mRNA and protein levels were significantly up-regulated in ayu monocytes/macrophages. Moreover, aIL-1β neutralization did not change phagocytic capability, but reduced bacterial killing capability in ayu head kidney-derived monocytes/macrophages. Therefore, aIL-1β may play an important role in immune response of ayu, especially, contributing to bacterial killing of monocytes/macrophages.
authors: School of Marine Sciences, Ningbo University, Fenghua Road 818, Jiangbei District, Ningbo 315211, China.
authors: Xin-Jiang
authors: XJ
authors: Lu
authors: Jiong
authors: J
authors: Chen
authors: Yu-Qing
authors: YQ
authors: He
authors: Yu-Hong
authors: YH
authors: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007274:Injections, Intraperitoneal; D053583:Interleukin-1beta / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D053526:Listonella / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D036441:Osmeriformes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D053583:Interleukin-1beta; D012333:RNA, Messenger