title: Binding of DC-SIGN to the hemagglutinin of influenza A viruses supports virus replication in DC-SIGN expressing cells.
abstract: Dendritic cells express lectins receptors, like DC-SIGN, which allow these cells to sense glycans that are present on various bacterial and viral pathogens. Interaction of DC-SIGN with carbohydrate moieties induces maturation of dendritic cells and promotes endocytosis of pathogens which is an important property of these professional antigen presenting cells. Uptake of pathogens by dendritic cells may lead to cross-presentation of antigens or infection of these cells, which ultimately results in activation of virus-specific T cells in draining lymph nodes. Little is known about the interaction of DC-SIGN with influenza A viruses. Here we show that a virus with a non-functional receptor binding site in its hemagglutinin, can replicate in cells expressing DC-SIGN. Also in the absence of sialic acids, which is the receptor for influenza A viruses, these viruses replicate in DC-SIGN expressing cells including human dendritic cells. Furthermore, the efficiency of DC-SIGN mediated infection is dependent on the extent of glycosylation of the viral hemagglutinin.
authors: Erasmus Medical Centre, Rotterdam, The Netherlands.
authors: Marine L B
authors: ML
authors: Hillaire
authors: Nella J
authors: NJ
authors: Nieuwkoop
authors: Adrianus C M
authors: AC
authors: Boon
authors: Gerrie
authors: G
authors: de Mutsert
authors: Stella E
authors: SE
authors: Vogelzang-van Trierum
authors: Ron A M
authors: RA
authors: Fouchier
authors: Albert D M E
authors: AD
authors: Osterhaus
authors: Guus F
authors: GF
authors: Rimmelzwaan
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D004285:Dogs; D005786:Gene Expression Regulation; D006031:Glycosylation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology; D053122:Influenza A Virus, H3N2 Subtype / Q000502:physiology; D009980:Influenza A virus / Q000502:physiology*; D007700:Kinetics; D037181:Lectins, C-Type / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014779:Virus Replication; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin