title: Effect of the leptin receptor Q223R polymorphism on the host transcriptome following infection with Entamoeba histolytica.
abstract: Resistance to amebiasis is associated with a polymorphism in the leptin receptor. Previous studies demonstrated that humans with the ancestral Q223 leptin receptor allele were nearly four times less likely to be infected with Entamoeba histolytica than those carrying the mutant R223 allele. We hypothesized that the Q223 allele protected against E. histolytica via STAT3-mediated transcription of genes required for mucosal immunity. To test this, mice containing the humanized LEPR Q or R allele at codon 223 were intracecally infected with E. histolytica. Susceptibility to amebiasis was assessed, and cecal tissues were analyzed for changes in gene expression. By 72 h postchallenge, all Q223 mice had cleared E. histolytica, whereas 39% of 223R mice were infected. Thirty-seven genes were differentially expressed in response to infection at 72 h, including proinflammatory genes (CXCL2, S100A8/9, PLA2G7, ITBG2, and MMP9) and functions pertaining to the movement and activity of immune cells. A comparison at 12 h postchallenge of infected Q223 versus R223 mice identified a subset of differentially expressed genes, many of which were closely linked to leptin signaling. Further analyses indicated that the Q223 gene expression pattern was consistent with a suppressed apoptotic response to infection, while 223R showed increased cellular proliferation and recruitment. These studies are the first to illuminate the downstream effects of leptin receptor polymorphisms on intestinal infection by E. histolytica. As such, they are important for the insight that they provide into this previously uncharacterized mechanism of mucosal immunity.
authors: Department of Medicine, Division of Infectious Diseases and International Health, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
authors: Nicole M
authors: NM
authors: Mackey-Lawrence
authors: Xiaoti
authors: X
authors: Guo
authors: Daniel E
authors: DE
authors: Sturdevant
authors: Kimmo
authors: K
authors: Virtaneva
authors: Matthew M
authors: MM
authors: Hernandez
authors: Eric
authors: E
authors: Houpt
authors: Alan
authors: A
authors: Sher
authors: Stephen F
authors: SF
authors: Porcella
authors: William A
authors: WA
authors: Petri
mesh_terms: D000483:Alleles; D000818:Animals; D004195:Disease Models, Animal; D004748:Entamoeba histolytica; D004749:Entamoebiasis / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D051379:Mice; D008810:Mice, Inbred C57BL; D011110:Polymorphism, Genetic; D054411:Receptors, Leptin / Q000235:genetics*; D059467:Transcriptome
chemical_list: D054411:Receptors, Leptin