title: Clustering subspecies of Aeromonas salmonicida using IS630 typing.
abstract: BACKGROUND
  The insertion element IS630 found in Aeromonas salmonicida belongs to the IS630-Tc1-mariner superfamily of transposons. It is present in multiple copies and represents approximately half of the IS present in the genome of A. salmonicida subsp. salmonicida A449.
  
  
  RESULTS
  By using High Copy Number IS630 Restriction Fragment Length Polymorphism (HCN-IS630-RFLP), strains of various subspecies of Aeromonas salmonicida showed conserved or clustering patterns, thus allowing their differentiation from each other. Fingerprints of A. salmonicida subsp. salmonicida showed the highest homogeneity while 'atypical' A. salmonicida strains were more heterogeneous. IS630 typing also differentiated A. salmonicida from other Aeromonas species. The copy number of IS630 in Aeromonas salmonicida ranges from 8 to 35 and is much lower in other Aeromonas species.
  
  
  CONCLUSIONS
  HCN-IS630-RFLP is a powerful tool for subtyping of A. salmonicida. The high stability of IS630 insertions in A. salmonicida subsp. salmonicida indicates that it might have played a role in pathoadaptation of A. salmonicida which has reached an optimal configuration in the highly virulent and specific fish pathogen A. salmonicida subsp. salmonicida.
authors: Institute of Veterinary Bacteriology, University of Bern, Länggassstrasse 122, Bern, Switzerland.
authors: Nicole
authors: N
authors: Studer
authors: Joachim
authors: J
authors: Frey
authors: Philippe
authors: P
authors: Vanden Bergh
mesh_terms: D048409:Aeromonas salmonicida / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D004251:DNA Transposable Elements; D005838:Genotype; D058889:Molecular Typing / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004251:DNA Transposable Elements