title: Determination of epidemiology of clinically isolated Cryptococcus neoformans strains in Japan by multilocus sequence typing.
abstract: Cryptococcus neoformans and Cryptococcus gattii are the causative agents of cryptococcosis. Despite its importance, our knowledge of the epidemiology of cryptococcosis in Japan remains limited. To establish an epidemiological database on cryptococcosis in Japan, we determined the genetic variability of 44 Japanese clinical isolates of C. neoformans (var. grubii: serotype A) by multilocus sequence typing (MLST). The strains were clinically isolated from 1992 to 2011 in 5 different areas of Japan (the Hokkaido region [n = 1], Kanto region [n = 32], Chubu region [n = 1], Kansai region [n = 1], and Kyushu region [n = 9]). According to the method recommended by the International Society for Human and Animal Mycology cryptococcal genotyping working group, 36 isolates (82%) were identified as sequence type (ST)46. The remaining strains belonged to ST45 (n = 1) and ST47 (n = 1), and 6 isolates belonged to novel independent STs. There was little geographic difference in the ST population. Our present data are still limited; however, because most clinical isolates showed the same MLST profile in Japan, applying the current MLST scheme for Cryptococcus may at times be insufficient for investigating the infection route among outbreak cases. To solve this problem, it may be necessary to investigate other gene loci or develop a novel method with greater discriminatory power. However, in cases in which a strain belongs to a minor ST, our data may serve as useful epidemiological information in Japan.
authors: Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors: Takashi
authors: T
authors: Umeyama
authors: Hideaki
authors: H
authors: Ohno
authors: Fujihiko
authors: F
authors: Minamoto
authors: Taeko
authors: T
authors: Takagi
authors: Chiyoko
authors: C
authors: Tanamachi
authors: Koichi
authors: K
authors: Tanabe
authors: Yukihiro
authors: Y
authors: Kaneko
authors: Satoshi
authors: S
authors: Yamagoe
authors: Kazuma
authors: K
authors: Kishi
authors: Takeshi
authors: T
authors: Fujii
authors: Hiromu
authors: H
authors: Takemura
authors: Hiroshi
authors: H
authors: Watanabe
authors: Yoshitsugu
authors: Y
authors: Miyazaki
mesh_terms: D001483:Base Sequence; D003453:Cryptococcosis / Q000453:epidemiology / Q000382:microbiology*; D056285:Cryptococcus gattii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D016533:Mycological Typing Techniques; D010802:Phylogeny; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins