title: ESI-MS/MS identification of a bradykinin-potentiating peptide from Amazon Bothrops atrox snake venom using a hybrid Qq-oaTOF mass spectrometer.
abstract: A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12.
authors: Center of Biomolecules Study Applied to Health, Fiocruz Rondônia, Oswaldo Cruz Foundation, Porto Velho, RO, Brazil. antonio.dnabrasil@gmail.com
authors: Antonio
authors: A
authors: Coutinho-Neto
authors: Cleópatra A S
authors: CA
authors: Caldeira
authors: Gustavo H M F
authors: GH
authors: Souza
authors: Kayena D
authors: KD
authors: Zaqueo
authors: Anderson M
authors: AM
authors: Kayano
authors: Rodrigo S
authors: RS
authors: Silva
authors: Juliana P
authors: JP
authors: Zuliani
authors: Andreimar M
authors: AM
authors: Soares
authors: Rodrigo G
authors: RG
authors: Stábeli
authors: Leonardo A
authors: LA
authors: Calderon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017837:Bothrops; D001920:Bradykinin; D003435:Crotalid Venoms / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D014757:Viper Venoms / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D003435:Crotalid Venoms; D009842:Oligopeptides; D010446:Peptide Fragments; D014757:Viper Venoms; C582054:pyroglutamyl-lysyl-tryptophyl-prolyl-arginyl-prolyl-glycyl-prolyl-glutamyl-isoleucyl-prolyl-proline; D001920:Bradykinin