title: Enhanced cytotoxicity for colon 26 cells using doxorubicin-loaded sorbitan monooleate (Span 80) vesicles.
abstract: Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicles (DVs) were thereafter added to Colon 26 cells. It was suggested, from the flow cytometric analysis and confocal laser microscopic observation, that DVs directly deliver DOX into the cytoplasm of Colon 26 cells. DVs showed the different delivery manner from the DOX-loaded liposomes (DLs). It is considered that the difference of delivery manner between DVs and DLs resulted in the difference of cytotoxicity (IC(50)); i.e. IC(50) values for DVs and DLs were 5 and > 30 μM, respectively. The results obtained herein would give the fundamental findings which can contribute to the improvement of formulation of conventional liposome-based carrier and its cytotoxicity.
authors: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Keita
authors: K
authors: Hayashi
authors: Tsuyoshi
authors: T
authors: Tatsui
authors: Toshinori
authors: T
authors: Shimanouchi
authors: Hiroshi
authors: H
authors: Umakoshi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy*; D004317:Doxorubicin / Q000494:pharmacology* / Q000627:therapeutic use; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006601:Hexoses / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D013778:Tetrazolium Salts; D013844:Thiazoles; D022161:Transport Vesicles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D006601:Hexoses; D008081:Liposomes; D013778:Tetrazolium Salts; D013844:Thiazoles; C018665:sorbitan monooleate; D004317:Doxorubicin; C022616:thiazolyl blue
keywords: Colon 26; Cytotoxicity; Span 80 vesicles