title: Periapical disease and bisphosphonates induce osteonecrosis of the jaws in mice.
abstract: Osteonecrosis of the jaw (ONJ) is a well-recognized complication of antiresorptive medications, such as bisphosphonates (BPs). Although ONJ is most common after tooth extractions in patients receiving high-dose BPs, many patients do not experience oral trauma. Animal models using tooth extractions and high BP doses recapitulate several clinical, radiographic, and histologic findings of ONJ. We and others have reported on rat models of ONJ using experimental dental disease in the absence of tooth extraction. These models emphasize the importance of dental infection/inflammation for ONJ development. Here, we extend our original report in the rat, and present a mouse model of ONJ in the presence of dental disease. Mice were injected with high dose zoledronic acid and pulpal exposure of mandibular molars was performed to induce periapical disease. After 8 weeks, quantitative and qualitative radiographic and histologic analyses of mouse mandibles were done. Periapical lesions were larger in vehicle-treated versus BP-treated mice. Importantly, radiographic features resembling clinical ONJ, including thickening of the lamina dura, periosteal bone deposition, and increased trabecular density, were seen in the drilled site of BP-treated animals. Histologically, osteonecrosis, periosteal thickening, periosteal bone apposition, epithelial migration, and bone exposure were present in the BP-treated animals in the presence of periapical disease. No difference in tartrate-resistant acid phosphatase (TRAP)+ cell numbers was observed, but round, detached, and removed from the bone surface cells were present in BP-treated animals. Although 88% of the BP-treated animals showed areas of osteonecrosis in the dental disease site, only 33% developed bone exposure, suggesting that osteonecrosis precedes bone exposure. Our data further emphasize the importance of dental disease in ONJ development, provide qualitative and quantitative measures of ONJ, and present a novel mouse ONJ model in the absence of tooth extraction that should be useful in further exploring ONJ pathophysiological mechanisms.
authors: Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, Los Angeles, CA, USA.
authors: Ben
authors: B
authors: Kang
authors: Simon
authors: S
authors: Cheong
authors: Thawinee
authors: T
authors: Chaichanasakul
authors: Olga
authors: O
authors: Bezouglaia
authors: Elisa
authors: E
authors: Atti
authors: Sarah M
authors: SM
authors: Dry
authors: Flavia Q
authors: FQ
authors: Pirih
authors: Tara L
authors: TL
authors: Aghaloo
authors: Sotirios
authors: S
authors: Tetradis
mesh_terms: D000818:Animals; D059266:Bisphosphonate-Associated Osteonecrosis of the Jaw / Q000473:pathology / Q000503:physiopathology; D050071:Bone Density Conservation Agents / Q000009:adverse effects* / Q000494:pharmacology; D004164:Diphosphonates / Q000009:adverse effects* / Q000494:pharmacology; D004195:Disease Models, Animal; D007093:Imidazoles / Q000009:adverse effects* / Q000494:pharmacology; D008297:Male; D008334:Mandible / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008963:Molar / Q000473:pathology / Q000503:physiopathology; D010483:Periapical Diseases / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D010521:Periosteum / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid