title: Faecal excretion dynamic during subacute oral exposure to different Pb species in Rattus norvegicus.
abstract: Faecal excretion is a basic means of detoxification upon ingestion of Pb-contaminated feed. In order to determine a time course of Pb elimination after oral exposure to two different forms of this heavy metal (lead acetate vs. phyto-bound Pb), a feeding study was carried out in experimental rats using the Pb phyto-hyperaccumulator Pistia stratiotes as a model diet. The effect of starvation on Pb excretion was further studied in rats that were fed plant material. Twelve Pb doses (7 μg Pb/1 g BW) were administered orally over a 5-week period. Faeces samples were collected 24 and 72 h post-exposure. Inductively coupled plasma optical emission spectrometry and electrothermal absorption spectrometry methods were used for determination of heavy metal concentrations. Up to 53 % of ingested Pb was rapidly eliminated from the exposed rats via faeces within 24 h after exposure. Faecal excretion in exposed rats differed significantly when compared to that of the control group. Fasting before exposure reduced Pb excretion by up to 50 %. Faecal excretions of both examined Pb forms exhibited almost identical patterns. Considerable differences were revealed concerning total excretion levels; lead acetate was excreted in amount greater extent than those of phytobound Pb. Results of our study suggest that Pb forms occurring in the P. stratiotes tissues are absorbed through the gastrointestinal tract to a greater extent than Pb from lead acetate. Therefore, higher portions of ingested Pb can be available for potential accumulation in tissues of exposed subjects.
authors: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, Prague 6, 165-21, Czech Republic.
authors: Zuzana
authors: Z
authors: Cadková
authors: Jiřina
authors: J
authors: Száková
authors: Daniela
authors: D
authors: Miholová
authors: Petr
authors: P
authors: Válek
authors: Zuzana
authors: Z
authors: Pacáková
authors: Jaroslav
authors: J
authors: Vadlejch
authors: Iva
authors: I
authors: Langrová
authors: Ivana
authors: I
authors: Jankovská
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005243:Feces / Q000737:chemistry*; D007854:Lead / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009942:Organometallic Compounds; D007854:Lead; C008261:lead acetate