title: Low-level laser therapy (LLLT) (660nm) alters gene expression during muscle healing in rats.
abstract: BACKGROUND
  The effects of LLLT were studied during muscle regeneration through gene expression.
  
  
  METHODS
  It was evaluated 10 and 50J/cm(2) doses during 7, 14 and 21days post cryoinjury, through histopathological analysis and mRNA MyoD, Myogenin, Vegf and Cox-2 expression.
  
  
  RESULTS
  Irradiated groups presented less inflammatory process than control group after 14 and 21days. Cox-2 levels were downregulated in all irradiated groups after 7, 14 and 21days. On day 7, both treated groups had a downregulation of Vegf levels, and an upregulation after 14 and 21days, mainly with 50J/cm(2). The MyoD levels were upregulated with high dose in all periods and with low dose after 21days. Myogenin expression was downregulated in both treated groups after 7days, and was upregulated with 10J/cm(2) after 21days.
  
  
  CONCLUSIONS
  These responses suggest that LLLT can improve the skeletal muscle regeneration through the gene expression stimulation.
authors: Department of Physiotherapy, Federal University of São Carlos - UFSCAR, Rodovia Washington Luís (SP-310), km 235, 13565-905 São Carlos, SP, Brazil. nataliacrod@yahoo.com.br
authors: Natalia Camargo
authors: NC
authors: Rodrigues
authors: Roberta
authors: R
authors: Brunelli
authors: Heloísa Sobreiro Selistre
authors: HS
authors: de Araújo
authors: Nivaldo Antonio
authors: NA
authors: Parizotto
authors: Ana Claudia Muniz
authors: AC
authors: Renno
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics; D005786:Gene Expression Regulation / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology* / Q000528:radiation effects*; D017570:MyoD Protein / Q000235:genetics; D018008:Myogenin / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000528:radiation effects*; D013977:Tibia; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D017570:MyoD Protein; D018008:Myogenin; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D051546:Cyclooxygenase 2