title: Re-emergence of dengue virus type 3 in Canton, China, 2009-2010, associated with multiple introductions through different geographical routes.
abstract: BACKGROUND
  Endemic dengue virus type 3 (DENV-3) infections have not been reported in Canton, China, since 1980. In March 2009, DENV-3 was isolated for the second time, occurring about 30 years after the previous circulation. In August, 3 other cases emerged. One much larger outbreak occurred again in 2010. To address the origin and particularly to determine whether the outbreaks were caused by the same viral genotype, we investigated the epidemiological and molecular characteristics of the introduction, spread and genetic microevolution of DENV-3 involved.
  
  
  RESULTS
  Three imported cases (index-1,2,3) separately traveled back from Vietnam, India and Tanzania, resulted in 1, 3 and 60 secondary autochthonous cases, respectively. In autochthonous cases, 64.6% positive in IgM anti-DENV and 18.6% in IgG from a total of 48 submitted serum samples, accompanied by 7 DENV-3 isolates. With 99.8%, 99.7%, and 100% envelope gene nucleotidic identity, 09/GZ/1081 from index-1 and endemic strain (09/GZ/1483) belonged to genotype V; 09/GZ/10616 from index-2 and endemic strains (09/GZ/11144 and 09/GZ/11194) belonged to genotype III Clade-A; and 10/GZ/4898 from index-3 and all four 2010 endemic DENV-3 strains belonged to genotype III Clade-B, respectively.
  
  
  CONCLUSIONS
  Both epidemiological and phylogenetic analyses showed that the 2010 outbreak of dengue was not a reemergence of the 2009 strain. Introductions of different genotypes following more than one route were important contributory factors for the 2009-2010 dengue epidemics/outbreaks in Canton. These findings underscore the importance of early detection and case management of imported case in preventing large-scale dengue epidemics among indigenous peoples of Canton.
authors: Department of Primary Public Health, Canton Center for Disease Control and Prevention, Canton, Guangdong Province, People's Republic of China.
authors: Huiying
authors: H
authors: Liang
authors: Lei
authors: L
authors: Luo
authors: Zhicong
authors: Z
authors: Yang
authors: Biao
authors: B
authors: Di
authors: Zhijun
authors: Z
authors: Bai
authors: Peng
authors: P
authors: He
authors: Qinlong
authors: Q
authors: Jing
authors: Xueli
authors: X
authors: Zheng
mesh_terms: D002681:China / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005838:Genotype; D005843:Geography; D006801:Humans; D007194:India / Q000453:epidemiology; D010802:Phylogeny; D013636:Tanzania / Q000453:epidemiology; D014744:Vietnam / Q000453:epidemiology