title: Development of apple certified reference material for quantification of organophosphorus and pyrethroid pesticides.
abstract: An apple certified reference material for the analysis of pesticide residues was issued by the National Metrology Institute of Japan. Organophosphorus and pyrethroid pesticides were sprayed on apples, and these were used as raw materials of certified reference material. The harvested apples were cut into small pieces, freeze-dried, pulverized, sieved, placed into 200 brown glass bottles (3g each), and sterilized by γ-irradiation. Stability and homogeneity assessment was performed, and the relative uncertainties due to instability (for an expiry date of 32 months) and inhomogeneity were 10.3-25.0% and 4.0-6.8%, respectively. The characterization was carried out using multiple analytical methods to ensure the reliability of analytical results; the values of target pesticides were obtained by isotope dilution mass spectrometry. Certified values were 2.28 ± 0.82 mg/kg for diazinon, 3.14 ± 0.79 mg/kg for fenitrothion, 1.55 ± 0.81 mg/kg for cypermethrin, and 2.81 ± 0.70 mg/kg for permethrin.
authors: National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Umezono, Tsukuba, Ibaraki 305-8563, Japan. t-ootake@aist.go.jp
authors: Takamitsu
authors: T
authors: Otake
authors: Takashi
authors: T
authors: Yarita
authors: Yoshie
authors: Y
authors: Aoyagi
authors: Youko
authors: Y
authors: Kuroda
authors: Masahiko
authors: M
authors: Numata
authors: Hitoshi
authors: H
authors: Iwata
authors: Masatoshi
authors: M
authors: Watai
authors: Hitoshi
authors: H
authors: Mitsuda
authors: Takashi
authors: T
authors: Fujikawa
authors: Hidekazu
authors: H
authors: Ota
mesh_terms: D005506:Food Contamination / Q000032:analysis; D027845:Malus / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods / Q000592:standards*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*; D012015:Reference Standards
chemical_list: D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D011722:Pyrethrins