title: Cytotoxic, genotoxic and antigenotoxic potencies of oligorutins.
abstract: Rutin has been enzymatically oligomerized by laccase from Trametes versicolor. Five fractions of oligomers were obtained from the monomers having high solubility in water, which can reach 351-times that of rutin. Cytotoxicity of rutin and oligorutin fractions was evaluated towards K562 cells. Oligorutin fractions showed a lower antiproliferative effect compared with its monomer. The genotoxic potential of rutin and oligorutin fractions was assessed, at the limit of the solubility of each molecule, using the comet test. None of the tested concentrations of either rutin or oligorutin fractions has showed a genotoxic effect. Similarly, the antigenotoxic effect of these flavonoids was tested using the same assay. The obtained results showed a higher ability of oligorutin fractions to reduce the genotoxicity induced by hydrogen peroxide compared with monomeric rutin.
authors: Laboratoire d'Ingénierie des Biomolécules, ENSAIA-INPL, Université de Lorraine, Vandoeuvre-lès-Nancy, France.
authors: G B
authors: GB
authors: Rhouma
authors: L
authors: L
authors: Chebil
authors: N
authors: N
authors: Mustapha
authors: M
authors: M
authors: Krifa
authors: K
authors: K
authors: Ghedira
authors: M
authors: M
authors: Ghoul
authors: L
authors: L
authors: Chékir-Ghédira
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D020552:Comet Assay; D003603:Cytotoxins / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D020014:K562 Cells; D042845:Laccase / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000494:pharmacology*; D012431:Rutin / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins; D011108:Polymers; D012431:Rutin; D006861:Hydrogen Peroxide; D042845:Laccase
keywords: Oligorutin; comet assay; cytotoxicity; solubility