title: [Corn plant DNA methylation pattern changes upon fractional UV-C irradiation].
abstract: Relationship of changes of methylation pattern of functionally different parts of DNA and chromosomal aberration yield was studied at the conditions of the fractionating of UV-C irradiation. Combination of restriction analysis (Hpall, MspI, MboI enzymes) with the subsequent raising of PCR (internal transcribed space ITS1, 1TS4 and inter simple sequence repeat - ISSR, 14b primers) was used. The got results testify to the changes in methylation pattern of satellite and transcription active part of DNA atan irradiation in the mode of fractionating and depending on fraction time ranges. The role of the methylation DNA pattern change in development of radiation damage and induction of organism protective reactions was discussed.
authors: A P
authors: AP
authors: Kravets
authors: D A
authors: DA
authors: Sokolova
authors: G S
authors: GS
authors: Vengzhen
authors: D M
authors: DM
authors: Grodzinskiĭ
mesh_terms: D002869:Chromosome Aberrations; D032461:Chromosomes, Plant; D019175:DNA Methylation / Q000528:radiation effects; D017931:DNA Primers; D004262:DNA Restriction Enzymes; D021901:DNA, Intergenic; D018744:DNA, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000528:radiation effects*; D014158:Transcription, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays; D003313:Zea mays / Q000235:genetics / Q000528:radiation effects*
chemical_list: D017931:DNA Primers; D021901:DNA, Intergenic; D018744:DNA, Plant; D005819:Genetic Markers; D004262:DNA Restriction Enzymes