title: Magnetic bead protocol for culturing Mycobacterium tuberculosis from sputum specimens.
abstract: A magnetic bead protocol and a standard centrifugation protocol yielded Mycobacterium tuberculosis in 40/50 sputum specimens in 12 ± 1 days and 11 ± 2 days, respectively (P > 0.05 by Student's t test). Manipulation took 35 ± 5 min and 45 ± 10 min, respectively (P < 0.05). The magnetic bead protocol could advantageously replace centrifugation for culturing M. tuberculosis from sputum.
authors: Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, Inserm 1095, Marseille, France.
authors: Ramzi
authors: R
authors: Ghodbane
authors: Michel
authors: M
authors: Drancourt
mesh_terms: D001431:Bacteriological Techniques; D002498:Centrifugation; D003470:Culture Media; D006801:Humans; D018189:Immunomagnetic Separation; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D003470:Culture Media