title: Fabrication of a thin-layer solid optical tissue phantom by a spin-coating method: pilot study.
abstract: Solid optical tissue phantoms (OTPs) have been widely used for many purposes. This study introduces a spin-coating method (SCM) to fabricate a thin-layer solid OTP (TSOTP) with epidermal thickness. TSOTPs are fabricated by controlling the spin speed (250 to 2500 rpm), absorber concentration (0.2% to 1.0%), and the number of layers. The results show that the thicknesses of the TSOTPs are homogeneous in the region of interest. The one-layer TSOTP achieves maximum and minimum thicknesses of 65±0.28 μm (250 rpm) and 5.1±0.17 μm (2500 rpm), respectively, decreasing exponentially as a function of the spin speed. The thicknesses of the multilayer TSOTPs increases as a function of the number of layers and are correlated strongly with the spin speed (R2≥0.95). The concentration of the OTP mixture does not directly affect the thickness of the TSOTP; however, the absorption coefficients exponentially increase as a function of absorber concentration (R2≥0.98). These results suggest that the SCM can be used to fabricate homogeneous TSOTPs with various thicknesses by controlling the spin speed and number of layers. Finally, a double-layer OTP that combines epidermal TSOTP and dermal OTP is manufactured as a preliminary study to investigate the practical feasibility of TSOTPs.
authors: Yonsei University, Department of Biomedical Engineering, 1 Yonseidae-gil, Wonju, Gangwon-do, 220-710, Republic of Korea.
authors: Yunjin
authors: Y
authors: Bae
authors: Taeyoon
authors: T
authors: Son
authors: Jihoon
authors: J
authors: Park
authors: Byungjo
authors: B
authors: Jung
mesh_terms: D004817:Epidermis / Q000033:anatomy & histology; D006801:Humans; D061848:Optical Imaging; D055620:Optical Phenomena; D019047:Phantoms, Imaging