title: [p16 gene CpG methylation in cervical squamous cell carcinoma and HPV16 infection in Uyghur women].
abstract: OBJECTIVE
  To determine the correlation between p16 gene CpG methylation sites in the promoter region and HPV16 infection in cervical squamous cell carcinoma in Xinjiang Uyghur women.
  
  
  METHODS
  MALDI-TOF MS was used quantitatively to analyze p16 gene promotor methylation status of CpG islands in 20 cervix squamous cell carcinomas and 20 corresponding non-cancerous tissues in Uyghur women. HPV16 infection was detected by polymerase chain reaction (PCR) in both groups.
  
  
  RESULTS
  Among the 16 CpG sites in the p16 gene promoter region, CpG1-2 and CpG 6 sites were different between the 2 groups, and the levels of CpG1-2 and CpG6 methylation sites in the cervical squamous cell carcinoma were higher than those in the control group. The presence of HPV16 infection was significantly different between the cervix squamous cell carcinoma tissue and non-cancerous tissues (P<0.05). There was no significant correlation between p16 gene CpG methylation sites and HPV16 infection of cervical squamous cell carcinoma in Uyghur women.
  
  
  CONCLUSIONS
  P16 gene CpG 1-2, CpG 6 hypermethylation and HPV16, which are independent of one another, play an important role in cervical squamous cell carcinogenesis in Uyghur women.
authors: Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.
authors: Min
authors: M
authors: Yuan
authors: Ailing
authors: A
authors: Li
authors: Lili
authors: L
authors: Yao
authors: Guqun
authors: G
authors: Shen
authors: Jingxin
authors: J
authors: Cheng
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D002681:China / Q000208:ethnology; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D019942:Genes, p16; D052162:Human papillomavirus 16; D006801:Humans; D030361:Papillomavirus Infections / Q000150:complications*; D011401:Promoter Regions, Genetic; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology