title: Do MAO A and MAO B utilize the same mechanism for the C-H bond cleavage step in catalysis? Evidence suggesting differing mechanisms.
abstract: The detailed molecular mechanism proposed for the MAO-catalyzed oxidation of amines has been controversial with the basic assumption that both MAO A and MAO B follow the same pathway for the C-H bond cleavage step. Using the mechanistic approach of investigation of electronic effects of various benzylamine ring substituents in experiments at pH 9.0, human MAO A exhibits a kinetic behavior characteristic of an H(+) abstraction, while human MAO B exhibits kinetic properties characteristic of a H(-) abstraction. These results lead to the conclusion that the assumption that MAO A and MAO B follow identical mechanisms is incorrect.
authors: Department of Biochemistry, Emory University Rollins Research Center, 1510 Clifton Road, Atlanta, GA 30322, USA. rorru@emory.edu
authors: R
authors: R
authors: Orru
authors: M
authors: M
authors: Aldeco
authors: D E
authors: DE
authors: Edmondson
mesh_terms: D000818:Animals; D001596:Benzylamines / Q000378:metabolism; D002384:Catalysis; D006801:Humans; D006865:Hydrogenation; D007700:Kinetics; D008995:Monoamine Oxidase / Q000737:chemistry* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001596:Benzylamines; C030796:benzylamine; D008995:Monoamine Oxidase