title: MicroRNA-25 functions as a potential tumor suppressor in colon cancer by targeting Smad7.
abstract: Because it is a member of the miR-106b~25 cluster, microRNA-25 (miR-25) is known to be dysregulated in human cancers. However, the expression and role of miR-25 in colon cancer remain unclear. In this study, miR-25 was found to be down-regulated in human colon cancer tissues when compared to those in matched, non-neoplastic mucosa tissues. Functional studies revealed that restoration of miR-25 expression inhibited cell proliferation and migration. In contrast, miR-25 inhibition could promote the proliferation and migratory ability of cells. Stable over-expression of miR-25 also suppressed the growth of colon cancer-cell xenografts in vivo. Furthermore, bioinformatic predictions and experimental validation were used to identify Smad7 as a direct target of miR-25. Functional reverse experiments indicated that the antitumor effects of miR-25 were probably mediated by its repression of Smad7. These results suggest that miR-25 may function as a tumor suppressor by targeting Smad7 in colon cancer. Thus, miR-25 may serve as a potential therapeutic agent or target for cancer therapy.
authors: Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Qiang
authors: Q
authors: Li
authors: Chaoxia
authors: C
authors: Zou
authors: Chendan
authors: C
authors: Zou
authors: Zhongjing
authors: Z
authors: Han
authors: Haifeng
authors: H
authors: Xiao
authors: Huiyan
authors: H
authors: Wei
authors: Wei
authors: W
authors: Wang
authors: Lei
authors: L
authors: Zhang
authors: Xueying
authors: X
authors: Zhang
authors: Qingchao
authors: Q
authors: Tang
authors: Chunjing
authors: C
authors: Zhang
authors: Ji
authors: J
authors: Tao
authors: Xishan
authors: X
authors: Wang
authors: Xu
authors: X
authors: Gao
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002465:Cell Movement; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008875:Middle Aged; D009368:Neoplasm Transplantation; D034622:RNA Interference; D051906:Smad7 Protein / Q000235:genetics* / Q000378:metabolism; D047368:Tumor Burden / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; C497806:SMAD7 protein, human; D051906:Smad7 Protein