title: Enhancement in statistical and image analysis for in situ µSXRF studies of elemental distribution and co-localization, using Dioscorea balcanica.
abstract: Synchrotron-based X-ray microfluorescence (µSXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e.g. single cells and tissues. Elements are mapped and quantified at sub-p.p.m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Košanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by µSXRF.
authors: DESY, Notkestrasse 85, Hamburg, Germany. tanja.ducic@desy.de,
authors: Tanja
authors: T
authors: Dučić
authors: Manuela
authors: M
authors: Borchert
authors: Aleksandar
authors: A
authors: Savić
authors: Aleksandar
authors: A
authors: Kalauzi
authors: Aleksandra
authors: A
authors: Mitrović
authors: Ksenija
authors: K
authors: Radotić
mesh_terms: D027742:Dioscorea / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D018547:Plant Stems / Q000737:chemistry*; D025341:Principal Component Analysis; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D014131:Trace Elements / Q000032:analysis*
chemical_list: D014131:Trace Elements
keywords: Dioscorea balcanica Košanin; X-ray fluorescence microscopy; image analysis; statistical analysis