title: Proteomic and profile analysis of the proteins laced with aragonite and vaterite in the freshwater mussel Hyriopsis cumingii shell biominerals.
abstract: Hyriopsis cumingii (Lea, Unionidae), a freshwater bivalve species widely distributed in China and commercially exploited for freshwater pearl production, was chosen as the reference model to investigate the protein signature in the organic scaffold matching calcium carbonate crystallization mode. This study takes advantage of different calcium carbonate habits production by the organism: aragonite in shell and pearl and vaterite in alternative pearl formation. Amino acid global composition and proteomics analysis have been undertaken to study the amino acid imbalance with respect to biominerals and microstructures. Forty peptides sequences were obtained by proteomics, of which ten are shared by all the different samples, nine are laced with aragonite; another nine with vaterite and twelve are related to pearls. Bioinformatics analysis allowed the peptides to be matched to the deduced protein sequences from EST databases and allowed functional assignment (e.g. scaffolding, strain strength, chitin binding or carbonic anhydrase function) to the proteins found in the different materials. Such panel of motifs tailored in vaterite and aragonite habits produced in a freshwater mollusk gives food for thought about organic control of the biomineralization processes.
authors: UMR CNRS 5178, Biologie des Organismes Marins et Ecosystèmes MNHN, CP 26, 43 rue Cuvier, 75231 Paris Cedex 05, France. berland@mnhn.fr
authors: Sophie
authors: S
authors: Berland
authors: Yufei
authors: Y
authors: Ma
authors: Arul
authors: A
authors: Marie
authors: Jean-Pierre
authors: JP
authors: Andrieu
authors: Laurent
authors: L
authors: Bedouet
authors: Qingling
authors: Q
authors: Feng
mesh_terms: D000595:Amino Acid Sequence; D060105:Animal Shells / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D049872:Bivalvia / Q000737:chemistry* / Q000378:metabolism; D002119:Calcium Carbonate / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics
chemical_list: D011506:Proteins; D002119:Calcium Carbonate