title: Effects of amylosucrase treatment on molecular structure and digestion resistance of pre-gelatinised rice and barley starches.
abstract: Structural modification of rice and barley starches with Neisseria polysaccharea amylosucrase (NpAS) was conducted, and relationship between structural characteristics and resistant starch (RS) contents of NpAS-treated starches was investigated. Pre-gelatinised rice and barley starches were treated with NpAS. NpAS-treated starches were characterised with respect to morphology, X-ray diffraction pattern, amylopectin branch-chain distribution, and RS content, and their structural characteristics were correlated to RS contents. Regardless of amylose contents of native starches, NpAS-treated (relative to native) starches possessed lower and higher proportions of shorter (DP 6-12) and intermediate (DP 13-36) amylopectin (AP) branch-chains, respectively. RS contents were higher for NpAS-treated starches relative to native starches, and maximum RS contents were obtained for NpAS-treated starches of waxy rice and barley genotypes. Amylose contents were not associated with RS contents of NpAS-treated starches. However, shorter and intermediate AP branch-chain portions were negatively and positively correlated to RS contents of NpAS-treated starches, respectively.
authors: Department of Food Science and Technology, Carbohydrate Bioproduct Research Center, Sejong University, Gunja-dong 98, Gwangjin-gu, Seoul 143-747, Republic of Korea.
authors: Bum-Su
authors: BS
authors: Kim
authors: Hyun-Seok
authors: HS
authors: Kim
authors: Jung-Sun
authors: JS
authors: Hong
authors: Kerry C
authors: KC
authors: Huber
authors: Jae-Hoon
authors: JH
authors: Shim
authors: Sang-Ho
authors: SH
authors: Yoo
mesh_terms: D000688:Amylose / Q000032:analysis; D001426:Bacterial Proteins / Q000737:chemistry*; D004063:Digestion; D005964:Glucosyltransferases / Q000737:chemistry*; D001467:Hordeum / Q000737:chemistry*; D015394:Molecular Structure; D009343:Neisseria / Q000201:enzymology; D012275:Oryza / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D013213:Starch; D000688:Amylose; D005964:Glucosyltransferases; C019700:amylosucrase