title: Marine actinobacterial mediated gold nanoparticles synthesis and their antimalarial activity.
abstract: Streptomyces sp LK-3 (JF710608) mediated Gold nanoparticles (Au-N-LK3) were found within the size range of 5-50 nm. Au-N-LK3 treatment in Plasmodium berghei ANKA (PbA) infected mice delayed the parasitemia rise (~6%) compared to PbA infection on 8 days post infection. Survivability of mice increases to ~85% in Au-N-LK3 treated mice in contrast to in PbA (~50%) infected mice in 8 dpi with respect to control. During Au-N-LK3 treatment in PbA infection, histomorphological analysis revealed as such no change in spleen and liver tissue during 8 dpi. Our results confirmed up-regulation of TGF-β and down-regulation of TNF-α in tissue and serum level in PbA infected Au-N-LK3 treated mice compared to PbA infection. No significant changes were found in the hatchability of Artemia embryos upto 8 mg. The results obtained suggest that the Au-N-LK3 possess anti-malarial activity and could be considered as a potential source for anti-malarial drug development.
  
  
  BACKGROUND
  These investigators present a method of marine actinobacteria mediated synthesis of gold nanoparticles, resulting in nanoparticles that possess anti-malarial activity and could be considered in future anti-malarial drug development.
authors: Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors: L
authors: L
authors: Karthik
authors: Gaurav
authors: G
authors: Kumar
authors: Tarun
authors: T
authors: Keswani
authors: Arindam
authors: A
authors: Bhattacharyya
authors: B Palakshi
authors: BP
authors: Reddy
authors: K V Bhaskara
authors: KV
authors: Rao
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology* / Q000627:therapeutic use; D016923:Cell Death / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D008099:Liver / Q000187:drug effects / Q000469:parasitology / Q000473:pathology; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D010962:Plasmodium berghei / Q000187:drug effects*; D012623:Seawater / Q000382:microbiology*; D013154:Spleen / Q000187:drug effects / Q000469:parasitology / Q000473:pathology; D013302:Streptomyces / Q000302:isolation & purification*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D000962:Antimalarials; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D006046:Gold
keywords: Cytokines; Gold nanoparticles; Plasmodium berghei ANKA; Streptomyces sp LK-3