title: Double nanohole optical trapping: dynamics and protein-antibody co-trapping.
abstract: A double nanohole in a metal film can optically trap nanoparticles such as polystyrene/silica spheres, encapsulated quantum dots and up-converting nanoparticles. Here we study the dynamics of trapped particles, showing a skewed distribution and low roll-off frequency that are indicative of Kramers-hopping at the nanoscale. Numerical simulations of trapped particles show a double-well potential normally found in Kramers-hopping systems, as well as providing quantitative agreement with the overall trapping potential. In addition, we demonstrate co-trapping of bovine serum albumin (BSA) with anti-BSA by sequential delivery in a microfluidic channel. This co-trapping opens up exciting possibilities for the study of protein interactions at the single particle level.
authors: Department of Electrical and Computer Engineering, University of Victoria, Victoria, BC, Canada V8W 3P6.
authors: Ana
authors: A
authors: Zehtabi-Oskuie
authors: Hao
authors: H
authors: Jiang
authors: Bryce R
authors: BR
authors: Cyr
authors: Douglas W
authors: DW
authors: Rennehan
authors: Ahmed A
authors: AA
authors: Al-Balushi
authors: Reuven
authors: R
authors: Gordon
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D052898:Optical Tweezers; D011137:Polystyrenes / Q000737:chemistry; D060066:Protein Interaction Maps; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000906:Antibodies; D011137:Polystyrenes; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide