title: 1H NMR-based metabolic fingerprinting of urine metabolites after consumption of lingonberries (Vaccinium vitis-idaea) with a high-fat meal.
abstract: The use of NMR metabolomics in clinical trials is growing; however, reports of postprandial experiments in humans are scarce. The present study investigated whether consumption of lingonberries as a supplement to an oil-rich meal modifies the postprandial fingerprints of human urine. Urine samples were analysed by (1)H NMR, and untargeted multivariate analysis was applied to the data for comprehensive fingerprinting. A clear separation of postprandial lingonberry meal samples was revealed. To evaluate statistical differences, a targeted approach was applied for the informative spectral areas. Significantly (p<0.05) increased levels of polyphenol metabolites, hippuric acid and 4-hydroxyhippuric acid, and decreased creatinine and dimethylamine levels were the major explanations for the grouping of the postprandial samples after the different meals. Thus, inclusion of polyphenol-rich lingonberry powder in a rapeseed oil-rich meal modifies the metabolic profile of urine which may be used to reveal both consumption of berries and health-promoting changes in the common metabolism.
authors: Department of Biochemistry and Food Chemistry, University of Turku, FI-20014 Turku, Finland.
authors: Henna-Maria
authors: HM
authors: Lehtonen
authors: Anni
authors: A
authors: Lindstedt
authors: Riikka
authors: R
authors: Järvinen
authors: Jari
authors: J
authors: Sinkkonen
authors: Gonçalo
authors: G
authors: Graça
authors: Matti
authors: M
authors: Viitanen
authors: Heikki
authors: H
authors: Kallio
authors: Ana M
authors: AM
authors: Gil
mesh_terms: D004041:Dietary Fats / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D019518:Postprandial Period; D014556:Urine / Q000737:chemistry*; D029801:Vaccinium vitis-idaea / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004041:Dietary Fats