title: Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs.
abstract: Cancer metastasis is a highly coordinated and dynamic multistep process in which cancer cells interact with a variety of host cells. Morphological studies have documented the association of circulating tumor cells with host platelets. Tumor cell-induced platelet aggregation (TCIPA) contributes significantly to hematogenous metastasis; however, the molecular mechanisms involved in breast cancer TCIPA are poorly characterized. In this study, MCF-7 metastatic human breast cancer cells induced dose-dependent aggregation of washed platelets. Four major platelet activation pathways, glycoprotein (GP)-Ib-IX, GPIIb/IIIa, thromboxane (TX)-A2 and adenosine diphosphate (ADP) were activated during TCIPA and were inhibited by their respective inhibitors, 7E3, SZ-1, aspirin and apyrase. Pretreatment of platelets with 7E3, SZ-1 or apyrase significantly inhibited TCIPA, while pretreatment with aspirin had no effect. Moreover, combined pretreatment of platelets with 7E3, SZ-1 and apyrase significantly inhibited TCIPA, compared to single inhibitors. Combinations of antiplatelet drugs may represent a promising strategy to prevent cancer metastasis.
authors: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006; ; Department of Oncology, Suzhou Xiangcheng People's Hospital, Suzhou 215131,  P.R. China ;
authors: Lian
authors: L
authors: Lian
authors: Wei
authors: W
authors: Li
authors: Zhen-Yu
authors: ZY
authors: Li
authors: Yi-Xiang
authors: YX
authors: Mao
authors: You-Tao
authors: YT
authors: Zhang
authors: Yi-Ming
authors: YM
authors: Zhao
authors: Kai
authors: K
authors: Chen
authors: Wei-Ming
authors: WM
authors: Duan
authors: Min
authors: M
authors: Tao
keywords: adenosine diphosphate; breast cancer; glycoprotein-IIb/IIIa; glycoprotein-Ib-IX; thromboxane A2; tumor cell-induced platelet aggregation