title: Aza-BODIPY: improved synthesis and interaction with soluble Aβ1-42 oligomers.
abstract: Dye-binding assays that are used to evaluate anti-aggregation ability of small molecule inhibitors towards amyloids are known to be prone to false-positive effects due to spectral overlaps between the dye and the inhibitor. Aza-BODIPY dye, which has both excitation and emission maxima above 600nm, exhibits a significant increase in its fluorescence intensity in the presence of soluble oligomers of Aβ1-42. These results indicate that aza-BODIPY could serve as a near-IR probe for detecting conformational changes of Aβ1-42 soluble oligomers in vitro, and it should eliminate false-positive effects that are associated with currently utilized thioflavin T-based dyes. In addition, a facile synthesis of aza-BODIPY has been developed, which might further expand the applications of this dye.
authors: Department of Chemistry, Texas Christian University, Fort Worth, TX 76129, USA.
authors: Laramie P
authors: LP
authors: Jameson
authors: Sergei V
authors: SV
authors: Dzyuba
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D001372:Aza Compounds / Q000737:chemistry* / Q000378:metabolism; D001896:Boron Compounds / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D005456:Fluorescent Dyes / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D016229:Amyloid beta-Peptides; D001372:Aza Compounds; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D011108:Polymers; C075222:amyloid beta-protein (1-42)