title: Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli.
abstract: Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, a P3HP biosynthetic pathway from glycerol was constructed in recombinant Escherichia coli. The genes for glycerol dehydratase and its reactivating factor (dhaB123 and gdrAB, from Klebsiella pneumoniae), propionaldehyde dehydrogenase (pduP, from Salmonella typhimurium), and polyhydroxyalkanoate synthase (phaC1, from Cupriavidus necator) were cloned and expressed in E. coli. After culture condition optimization, the final engineered strain accumulated 10.1 g/L P3HP (46.4% of the cell dry weight) using glycerol and glucose as cosubstrates in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without addition of any expensive precursor.
authors: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors: Qi
authors: Q
authors: Wang
authors: Peng
authors: P
authors: Yang
authors: Changshui
authors: C
authors: Liu
authors: Yongchang
authors: Y
authors: Xue
authors: Mo
authors: M
authors: Xian
authors: Guang
authors: G
authors: Zhao
mesh_terms: D004926:Escherichia coli / Q000502:physiology*; D005990:Glycerol / Q000378:metabolism*; D011422:Propionates / Q000302:isolation & purification* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics
chemical_list: D011422:Propionates; D011994:Recombinant Proteins; D005990:Glycerol