title: Identification, cloning and characterization of BmP41, a common antigenic protein of Babesia microti.
abstract: Babesia microti is a rodent tick-borne blood parasite and the major causative agent of emerging human babesiosis. Here, we identified a candidate of common antigenic protein BmP41 of B. microti by serological screening of cDNA library of human-pathogenic Gray strain with antisera against rodent Munich strain. Immunofluorescent antibody test using mouse anti-recombinant BmP41 (rBmP41) serum revealed that native BmP41 was expressed in each of the developmental stages of B. microti merozoites. An enzyme-linked immunosorbent assay (ELISA) using rBmP41 detected specific antibodies in sera from hamsters infected with B. microti Gray strain and mice infected with B. microti Munich strain. Taken together, BmP41 could be a promising universal serological marker for diagnosis of human babesiosis.
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors: Tatsunori
authors: T
authors: Masatani
authors: Hideo
authors: H
authors: Ooka
authors: Mohamad A
authors: MA
authors: Terkawi
authors: Shinuo
authors: S
authors: Cao
authors: Yuzi
authors: Y
authors: Luo
authors: Masahito
authors: M
authors: Asada
authors: Kei
authors: K
authors: Hayashi
authors: Yoshifumi
authors: Y
authors: Nishikawa
authors: Xuenan
authors: X
authors: Xuan
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D015703:Antigens, CD / Q000235:genetics*; D041001:Babesia microti / Q000235:genetics*; D001404:Babesiosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology*; D015415:Biomarkers / Q000097:blood*; D015153:Blotting, Western; D000071178:CD48 Antigen; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000235:genetics; D051379:Mice
chemical_list: D000913:Antibodies, Protozoan; D015703:Antigens, CD; D015415:Biomarkers; D000071178:CD48 Antigen; D017931:DNA Primers; D018076:DNA, Complementary; D007106:Immune Sera