title: Porcine skeletal muscle differentially expressed gene ATP5B: molecular characterization, expression patterns, and association analysis with meat quality traits.
abstract: The 2-DE/MS-based proteomics approach was used to investigate the differences of porcine skeletal muscle, and ATP5B was identified as one differential expression protein. In the present study, ATP5B gene was further cloned by RT-PCR, the sequence was analyzed using the bioinformatics method, and the mRNA expression was detected by qRT-PCR. Sequence analysis showed that the porcine ATP5B gene contains an ORF encoding 528-amino-acid residues with 49 and 166 nucleotides in the 5' and 3' UTRs, respectively. The mRNA of ATP5B was widely expressed in all 14 tissues tested, but especially highly expressed in parorchis and fat. The expression pattern of ATP5B was similar in Large White and Meishan breeds, showing that the expression was upregulated by 3 days after birth and downregulated during postnatal development of skeletal muscle. Comparing the two breeds, the mRNA abundance of ATP5B in Large White was more highly expressed than in Meishan at all developmental stages (P < 0.05). Moreover, a synonymous mutation, G75A in exon 8, was identified and association analysis with the traits of meat quality showed that it was significantly associated with the RLF, FMP, IFR, IMF, and IMW (P < 0.05). These results suggested that ATP5B probably plays a key role in porcine skeletal muscle development and may provide further insight into the molecular mechanisms responsible for breed-specific differences in meat quality.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors: Haixia
authors: H
authors: Xu
authors: Yongjie
authors: Y
authors: Xu
authors: Xiaojuan
authors: X
authors: Liang
authors: Yanbo
authors: Y
authors: Wang
authors: Fangfang
authors: F
authors: Jin
authors: Dengying
authors: D
authors: Liu
authors: Yun
authors: Y
authors: Ma
authors: Hongyu
authors: H
authors: Yuan
authors: Xinqiang
authors: X
authors: Song
authors: Wenxian
authors: W
authors: Zeng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001947:Breeding; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005787:Gene Frequency; D008297:Male; D008460:Meat; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development
chemical_list: D012333:RNA, Messenger; D025261:Mitochondrial Proton-Translocating ATPases