title: Development and application of nanoparticles synthesized with folic acid conjugated soy protein.
abstract: In this study, soy protein isolate (SPI) was conjugated with folic acid (FA) to prepare nanoparticles for target-specific drug delivery. Successful conjugation was evidenced by UV spectrophotometry and primary amino group analysis. An increase in count rate by at least 142% was observed in FA-SPI nanoparticles compared to the nonconjugated ones, whereas the particle size was decreased upon FA conjugation. This was probably attributed to the substitution of positively charged lysine residues by the FA backbone. The ζ-potential ranged from -36 to -42 mV depending on the conjugation degree, indicating desirable dispersion stability. Curcumin as a model drug was encapsulated successfully into FA-SPI nanoparticles, evidenced by X-ray diffraction study. The highest encapsulation and loading efficiencies were around 92.7% and 5.4%, respectively, which were significantly higher (P < 0.05) than those with nonconjugated SPI nanoparticles. In addition, a faster and more complete release of curcumin was observed for FA-SPI nanoparticles in PBS/Tween 20 buffer. Cell culture study showed that conjugation of FA resulted in an increase in cellular uptake by at most 93% in Caco-2 cells. These results suggested that FA-SPI is a potential wall material for encapsulation and enhanced delivery of anticancer drugs.
authors: Department of Nutrition and Food Science, University of Maryland , 0112 Skinner Building, College Park, Maryland 20742, United States.
authors: Zi
authors: Z
authors: Teng
authors: Yangchao
authors: Y
authors: Luo
authors: Thomas
authors: T
authors: Wang
authors: Boce
authors: B
authors: Zhang
authors: Qin
authors: Q
authors: Wang
mesh_terms: D018938:Caco-2 Cells; D003474:Curcumin / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D030262:Soybean Proteins / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D030262:Soybean Proteins; D005492:Folic Acid; D003474:Curcumin