title: [Primary study on protective effect of mulberry extracts on Abeta25-35-induced PC12 cells injury].
abstract: OBJECTIVE
  To investigate the neuroprotective effect of mulberry extracts (ME) against Abeta25-35-induced injury in cultured PC12 cells and explore the possible mechanisms involved.
  
  
  METHODS
  PC12 cells at logarithmic growth phase were divided into the normal control group, Abeta25-35 group (20 micromol/L for 24h) and ME pretreating groups at the concentrations of 25, 50, 100, 200, 400, 800 microg/ml. Cell viability was determined by MTT assay. On that basis, PC12 cells were divided into the normal control group, ME group (200 microg/ml ME incubation alone for 24h), Abeta25-35 group (20 micromol/L for 24h) and ME plus Abeta25-35 group (after pretreatment with 200 microg/ml ME for 24h, cells were exposed to 201 micromol/L Abeta25-35 for another 24h). Intracellular reactive oxidative species (ROS) was measured by using the fluorescent DCFH-DA and the apoptotic cells were observed by Hoechst33342 staining method.
  
  
  RESULTS
  (1) The results showed that exposure of PC12 cells to Abeta22-35 (20 micromol/L) for 24h induced notably neuronal injury (P < 0.05) as compared with the control group, while pretreatment with 100 or 200 microg/ml ME almost completely reversed Abeta25-35 induced neuronal injury (P < 0.05). (2) There were no remarkable changes in ROS levels and apoptosis rate of ME group as compared with the control group (P > 0.05). Compared with the control group, exposure to 20 micromol/L Abeta25-35 for 24h resulted in a significant decrease in cell viability (P < 0.05) while increase in ROS levels and cell apoptosis rate (P < 0.05). But pretreatment of PC12 cells with 200 microg/ml ME could counteract ROS formation and inhibit apoptosis (P < 0.05).
  
  
  CONCLUSIONS
  These results suggest that mulberry extracts rich in phenolics and anthocyanins could alleviate Abeta25-35-induced injury in PC12 cells, which might be associated with the antioxidative and antiapoptosis effects.
authors: Department of Nutrition and Food Hygiene, School of Public Health, Guangxi Medical University, Nanning 530021, China. nannanxjwstu@163.com
authors: Nan
authors: N
authors: Song
authors: Wei
authors: W
authors: Pang
authors: Hongpeng
authors: H
authors: Yang
authors: Long
authors: L
authors: Tan
authors: Jingming
authors: J
authors: Fu
authors: Haiqiang
authors: H
authors: Li
authors: Yugang
authors: Y
authors: Jiang
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D031625:Morus / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C074636:amyloid beta-protein (25-35)