title: [Analysis of the frequency of isolation and drug resistance of microorganisms isolated from the airways of adult CF patients treated in the Institute of Tuberculosis and Lung Disease during 2008-2011].
abstract: BACKGROUND
  Cystic fibrosis (CF) is the most common genetic autosomal recessive genetic disease. The most serious symptoms are observed in the lungs. Recurrent respiratory infections are the main causes of the hospitalizations and deaths of cystic fibrosis patients. Pathogens that commonly infect the airways of adult CF patients include Staphylococcus aureus and Pseudomonas aeruginosa. The aim of this study was to analyse the microorganisms cultured from the airways of adult CF patients and to test the antimicrobial resistance of the most frequently isolated bacteria.
  
  
  METHODS
  In this study, 1422 isolates of 89 CF patients were collected during a 4-year period. The microorganisms were cultured and identified according to standard microbiological procedures. Identification and drug susceptibility were performed in an automatic system Phoenix (BD), Vitek2Compact (bioMérieux), and disk-diffusion method by Kirby-Bauer.
  
  
  RESULTS
  Among the 1422 strains the most frequent pathogens were Pseudomonas aeruginosa (55.6%) and Staphylococcus aureus (37.8%). A total of 482 (61.0%) strains of 790 isolates of P. aeruginosa, were identified as P. aeruginosa mucoid phenotype. The isolates with mucoid phenotypes were more susceptible to antibiotics than non-mucoid. Eighty-six strains of S. aureus showed resistance to methicillin (MRSA), which accounted for 16.0% of all strains of S. aureus.
  
  
  CONCLUSIONS
  The analysis of microbiological materials from adult CF patients treated in IGiChP allowed the determination of the prevalence of potentially pathogenic microorganisms. The data obtained are consistent with the literature.
authors: Zakład Mikrobiologii, Instytut Gruźlicy i Chorób Płuc w Warszawie Kierownik. a.pacholczyk@igichp.edu.pl
authors: Agnieszka
authors: A
authors: Iwańska
authors: Joanna
authors: J
authors: Nowak
authors: Wojciech
authors: W
authors: Skorupa
authors: Ewa
authors: E
authors: Augustynowicz-Kopeć
mesh_terms: D000046:Academic Medical Centers; D000047:Academies and Institutes; D000328:Adult; D000368:Aged; D003550:Cystic Fibrosis / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006304:Health Status; D006801:Humans; D007297:Inpatients / Q000706:statistics & numerical data; D008297:Male; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification* / Q000472:pathogenicity; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000472:pathogenicity; D012189:Retrospective Studies