title: MicroRNA (miRNA) expression profiling of peripheral blood samples in multiple myeloma patients using microarray.
abstract: MicroRNAs (miRNAs) are mostly located at cancer-associated genomic regions or in fragile sites, suggesting their important role in the pathogenesis of human cancers. Multiple myeloma (MM) is a cancer of plasma cells, the third most common cancer of the blood after lymphoma and leukaemia. There are several published reports on miRNAs in MM, however most used bone marrow rather than peripheral blood samples. The aim of this study is to characterise miRNA expression in normal and MM patients using peripheral blood samples as it is less invasive and is readily available from patients. Blood samples from 35 MM patients were analysed using the microarray method. We identified up-regulation of 36 miRNAs (57%) and down-regulation of 27 miRNAs (43%). We also identified the CCND2, HMGA2 and IGF1R genes were among the highly predictive target genes (P(CT) > 0.80) for most of the deregulated miRNAs. These genes are known to play important roles in MM as well as other cancers. Five miRNAs (let-7c, miR-16, miR- 449, miR-181a and miR-181b) were found to exhibit similar expression patterns (p < 0.05) in peripheral blood when compared to data obtained by using bone marrow aspirates from MM patients in other studies. In conclusion, our study has demonstrated that miRNAs are also present and differentially expressed in the peripheral blood of MM patients compared to controls and may potentially serve as candidate tumour biomarkers in MM. In particular, let-7c and miR-16 have been shown to be significantly expressed in the bone marrow.
authors: Molecular Diagnostics and Protein Unit, Specialised Diagnostics Centre, Institute for Medical Research, Kuala Lumpur. yusnita@imr.gov.my
authors: Yyusnita
authors: Norsiah
authors: I
authors: I
authors: Zakiah
authors: K M
authors: KM
authors: Chang
authors: V S
authors: VS
authors: Purushotaman
authors: Z
authors: Z
authors: Zubaidah
authors: R
authors: R
authors: Jamal
mesh_terms: D000328:Adult; D000368:Aged; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: C505362:MIRN16 microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human