title: Effects of oral deoxynivalenol exposure on immune-related parameters in lymphoid organs and serum of mice vaccinated with porcine parvovirus vaccine.
abstract: Mice were exposed to deoxynivalenol (DON) via drinking water at a concentration of 2 mg/L for 36 days. On day 8 of treatment, inactivated porcine parvovirus vaccine (PPV) was injected intraperitoneally. The relative and absolute weight of the spleen was significantly decreased in the DON-treated group (DON). Antibody titers to parvovirus in serum were 47.9 ± 2.4 in the vaccination group (Vac), but 15.2 ± 6.5 in the group treated with DON and vaccine (DON + Vac). The IgA and IgG was not different in the DON, Vac an,d DON + Vac groups. IgM was significantly lower only in the DON + Vac group. However IgE was significantly increased in the Vac and DON + Vac group, but no change was observed between the Vac and DON + Vac groups. The concentrations of IL-2, IL-4, GM-CSF, MCP-1 and Rantes in serum, and IL-1α in mesenteric lymph node and MIP-1β in spleen were significantly increased by DON treatment compared to control. The concentrations of IL-2, IL-5, IL-6, IL-9, IL-12, IL-13 and Rantes in thymus, of IL-2 in spleen, and of IL-1α, IL-1β, IL-3, IL-5, IL-10, IL-17, G-CSF, GM-CSF and MCP-1 in mesenteric lymph nodes were significantly decreased in mice compared to those in the Vac group, while concentrations of IL-1α, IL-2, IL-9, IL-13,G-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1α and TNF-α were significantly increased in serum compared to the Vac group. In conclusion, the results presented here indicate that exposure to DON at 2.0 mg/L via drinking water can disrupt the immune response in vaccinated mice by modulating cytokines and chemokines involved in their immune response to infectious disease.
authors: Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Byung-Kook
authors: BK
authors: Choi
authors: Sang-Hee
authors: SH
authors: Jeong
authors: Joon-Hyung
authors: JH
authors: Cho
authors: Hyo-Sook
authors: HS
authors: Shin
authors: Seong-Wan
authors: SW
authors: Son
authors: Young-Keun
authors: YK
authors: Yeo
authors: Hwan-Goo
authors: HG
authors: Kang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016207:Cytokines / Q000097:blood; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D008198:Lymph Nodes / Q000187:drug effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D028323:Parvovirus, Porcine / Q000276:immunology*; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects; D014255:Trichothecenes / Q000008:administration & dosage*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D007166:Immunosuppressive Agents; D014255:Trichothecenes; D014765:Viral Vaccines; C007262:deoxynivalenol