title: Expression and role of the genes involved in the transport of bile acids in the liver and kidneys in mice.
abstract: BACKGROUND
  Bile acids are synthesized in the liver from cholesterol.
  
  
  METHODS
  This study investigated the impact and expression of different carriers of bile acid in the liver and kidneys. Eight-week-old male mice were used, which were fed for 15 days and divided into two groups: 15 mice fed with standard diet (control group) and another 15 mice fed with a rich diet of 5% cholesterol (second group). Bile acid dosage was based on their oxidation by 7α hydroxyl-steroid dehydrogenize. The mRNA expression was quantitatively analyzed by the real time of polymerase chain reaction (RT-PCR), and the expression of the renal carrier bile acid protein was analyzed by Western blot.
  
  
  CONCLUSIONS
  The expression of bile salt export pump involved in the uptake of bile acids in the basolateral membrane of hepatocytes revealed no differences between the two groups of mice. However, the expression of multidrug resistance-associated protein 2 was reduced in mice of the second group. Moreover, the expressions of organic anion transporting polypeptide 4, organic anion transporting polypeptide 1, and sodium taurocholate co-transporting polypeptide (Ntcp) involved in the uptake of bile acids in the apical pole of hepatocytes are suppressed in mice of the second group. The expression of multidrug resistance-associated protein 3 involved in the secretion of bile acids in the apical membrane of hepatocytes revealed no significant differences between the two groups. In mice of the second group, blood concentration of bile acids on the last day was increased. In those mice, the expression of intestinal bile acid transporter was reduced in the kidneys compared with the control mice.
authors: Departement de Physiologie Animale, Faculte des Sciences et Techniques, Universite d’Abomey Calavi, 01 BP, 4521 UAC-Cotonou, Republique du Benin. eattakpa@yahoo.fr
authors: Eugène S
authors: ES
authors: Attakpa
authors: Naguibou M
authors: NM
authors: Djibril
authors: Farid
authors: F
authors: Baba-Moussa
authors: Ganiou
authors: G
authors: Yessoufou
authors: Alphonse
authors: A
authors: Sezan
mesh_terms: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001647:Bile Acids and Salts / Q000235:genetics* / Q000378:metabolism*; D001693:Biological Transport, Active / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D017931:DNA Primers; D030541:Databases, Genetic; D004032:Diet; D015870:Gene Expression / Q000502:physiology*; D022781:Hepatocytes / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D027381:Liver-Specific Organic Anion Transporter 1; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis / Q000235:genetics; D029363:Organic Anion Transporters, Sodium-Dependent / Q000096:biosynthesis / Q000235:genetics; D029362:Organic Anion Transporters, Sodium-Independent / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D027981:Symporters / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; D001647:Bile Acids and Salts; D017931:DNA Primers; D027381:Liver-Specific Organic Anion Transporter 1; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D029363:Organic Anion Transporters, Sodium-Dependent; D029362:Organic Anion Transporters, Sodium-Independent; D027981:Symporters; C490549:Abcb11 protein, mouse; C498887:Slco1b2 protein, mouse; C071908:sodium-bile acid cotransporter