title: A new mouse model of inducible, chronic retinal ganglion cell dysfunction not associated with cell death.
abstract: OBJECTIVE
  To develop a mouse model of inducible, chronic retinal ganglion cell (RGC) dysfunction not associated with cell death.
  
  
  METHODS
  Eighteen C57BL/6J mice were longitudinally tested with pattern electroretinogram (PERG) and spectral-domain optical coherence tomography (OCT) before and after aspiration of the contralateral superior colliculus (SC), which removed terminals of optic tract axons and the superficial layers of the SC. At the 4-month end points, retinas were harvested for Brn3b immunostaining and BDNF immunoblotting.
  
  
  RESULTS
  The PERG lost approximately 60% of its baseline amplitude (P < 0.01) within the first day after lesion, and remained at a reduced level over 4 months. At the end point, the density of Brn3b-positive RGCs was normal, but their nucleus size was reduced by approximately 24% (P < 001). OCT measurements showed thinning of the inner, but not outer, retina by approximately 9% (P < 0.01) starting 10 to 20 days after lesion. Retinal nerve fiber layer thickness was unchanged. At the end point, retinal homogenates showed a substantial overexpression of BDNF protein level.
  
  
  CONCLUSIONS
  Mechanical SC lesion in adult mice results in a rapid, chronic loss of RGC electrical responsiveness that is followed by cell shrinkage but not cell death. The SC-lesion mouse represents a new, inducible model that allows investigating stages and mechanisms of RGC dysfunction without the confounding effects of cell death that are common in the existing models of optic neuropathies and optic nerve lesions.
authors: Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.
authors: Xu
authors: X
authors: Yang
authors: Tsung-Han
authors: TH
authors: Chou
authors: Marco
authors: M
authors: Ruggeri
authors: Vittorio
authors: V
authors: Porciatti
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D016923:Cell Death / Q000502:physiology*; D002908:Chronic Disease; D004195:Disease Models, Animal; D004596:Electroretinography; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D012164:Retinal Diseases / Q000473:pathology / Q000503:physiopathology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D013477:Superior Colliculi / Q000293:injuries / Q000502:physiology; D041623:Tomography, Optical Coherence