title: Knockdown of hepatic aquaglyceroporin-9 alleviates high fat diet-induced non-alcoholic fatty liver disease in rats.
abstract: Non-alcoholic fatty liver disease (NAFLD) has emerged as a common public health problem that can progress to end-stage liver disease. Although the function of aquaglyceroporin-9 (AQP9) as a glycerol channel has been proven, the mechanism of AQP9 in the development of NAFLD is poorly understood. To investigate the effect of hepatic glycerol uptake inhibition, we utilized a lentivirus-associated RNA interference technique to knock down the expression of hepatic AQP9 in high fat diet (HFD)-induced NAFLD in rodents. Male Sprague-Dawley rats were fed with a standard chow diet or HFD for 8 weeks. Rats fed a HFD were separated into 3 groups: HFD, control and transfection group, which were injected with equivalent amounts of PBS, lentivirus-scramble and lentivirus-shRNA-AQP9 through the hepatic portal vein. The results showed a remarkable decrease in the expression of AQP9 mRNA and protein in liver for the transfection group. Furthermore, less hepatic steatosis occurred when compared to the HFD and control groups after 8weeks. Our results indicate a potential role for AQP9 in the pathogenesis of hepatic steatosis.
authors: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, PR China.
authors: Can
authors: C
authors: Cai
authors: Chuan
authors: C
authors: Wang
authors: Wen
authors: W
authors: Ji
authors: Bo
authors: B
authors: Liu
authors: Yujun
authors: Y
authors: Kang
authors: Zaimin
authors: Z
authors: Hu
authors: Zheng
authors: Z
authors: Jiang
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000235:genetics / Q000502:physiology*; D059305:Diet, High-Fat / Q000009:adverse effects*; D015536:Down-Regulation / Q000235:genetics; D005234:Fatty Liver / Q000209:etiology* / Q000235:genetics*; D005822:Genetic Vectors; D016086:Lentivirus; D008099:Liver / Q000378:metabolism; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C491637:Aqp9 protein, rat; D020346:Aquaporins; D012333:RNA, Messenger; D034741:RNA, Small Interfering