title: Macrophage migration inhibitory factor is necessary for the lipo-oligosaccharide-induced response by modulation of Toll-like receptor 4 in monocytes from GBS patients.
abstract: Previous studies have suggested that macrophage migration inhibitory factor (MIF) may play a critical role in the pathogenesis of Guillain-Barre syndrome (GBS); however, its definite mechanism remains unknown. In this study, we prepared the monocytes from the peripheral blood mononuclear cells (PBMCs) of GBS patients and the controls. Lipo-oligosaccharide (LOS) from Campylobacter jejuni was used as the stimulus of the monocytes in vitro and siRNA-MIF was used to explore the roles of MIF in LOS-induced response. Significantly, silencing of MIF attenuated the LOS-induced up-regulation of Toll-like receptor 4 (TLR4) and translocation of NF-кB into the nucleus; we also observed the up-regulation of IL-12p40, TNF-α, IL-6, CXCL8 and CCL5 in GBS monocytes with LOS stimulus; and siRNA-MIF overrided the effects of LOS on the production of the TNF-α, IL-6, and CXCL8. Conclusively, our study provides evidences that MIF may participate in the pathogenesis of GBS by modulating the LOS-induced response through TLR4 signaling pathway.
authors: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors: Yu-Zhong
authors: YZ
authors: Wang
authors: Fa-Fa
authors: FF
authors: Tian
authors: Hao
authors: H
authors: Liu
authors: Wei
authors: W
authors: Zhang
authors: Jing
authors: J
authors: Li
authors: Bo
authors: B
authors: Xiao
authors: Wen-Bin
authors: WB
authors: Zhou
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D020275:Guillain-Barre Syndrome / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000037:antagonists & inhibitors / Q000502:physiology*; D008070:Lipopolysaccharides / Q000502:physiology*; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051197:Toll-Like Receptor 4 / Q000502:physiology*; D055815:Young Adult
chemical_list: D008070:Lipopolysaccharides; D008263:Macrophage Migration-Inhibitory Factors; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; C023023:lipid-linked oligosaccharides; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human