title: Activated human nasal epithelial cells modulate specific antibody response against bacterial or viral antigens.
abstract: Nasal mucosa is an immune responsive organ evidenced by eliciting both specific local secretory IgA and systemic IgG antibody responses with intra-nasal administration of antigens. Nevertheless, the role of nasal epithelial cells in modulating such responses is unclear. Human nasal epithelial cells (hNECs) obtained from sinus mucosa of patients with chronic rhinosinusitis were cultured in vitro and firstly were stimulated by Lactococcus lactis bacterium-like particles (BLPs) in order to examine their role on antibody production. Secondly, both antigens of immunodominant protein IDG60 from oral Streptococcus mutans and hemagglutinin (HA) from influenza virus were tested to evaluate the specific antibody response. Stimulated hNECs by BLPs exhibited a significant increase in the production of interleukin-6 (IL-6), and thymic stromal lymphopoietin (TSLP). Conditioned medium of stimulated hNECs has effects on enhancing the proliferation of CD4+ T cells together with interferon-γ and IL-5 production, increasing the costimulatory molecules on dendritic cells and augmenting the production of IDG60 specific IgA, HA specific IgG, IgA by human peripheral blood lymphocytes. Such production of antigen specific IgG and IgA is significantly counteracted in the presence of IL-6 and TSLP neutralizing antibodies. In conclusion, properly stimulated hNECs may impart immuno-modulatory effects on the antigen-specific antibody response at least through the production of IL-6 and TSLP.
authors: Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: Chiou-Yueh
authors: CY
authors: Yeh
authors: Te-Huei
authors: TH
authors: Yeh
authors: Chiau-Jing
authors: CJ
authors: Jung
authors: Pei-Lin
authors: PL
authors: Chen
authors: Huei-Ting
authors: HT
authors: Lien
authors: Jean-San
authors: JS
authors: Chia
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000276:immunology*; D002478:Cells, Cultured; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D005260:Female; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007155:Immunologic Factors / Q000276:immunology; D015848:Interleukin-5 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D012220:Rhinitis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D012852:Sinusitis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D001426:Bacterial Proteins; D006388:Hemagglutinins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007155:Immunologic Factors; D015848:Interleukin-5; D015850:Interleukin-6