title: Quercetin induces apoptosis via the mitochondrial pathway in KB and KBv200 cells.
abstract: In this study, anticancer activities of six compounds of flavonoids were investigated in human epidermoid carcinoma KB and KBv200 cells. Among these compounds, quercetin and acacetin showed strong inhibition of cell growth in KB and KBv200 cells. IC50 values of quercetin against KB and KBv200 cells were 17.84 ± 4.14 and 18.94 ± 4.75 μM, respectively. The IC50 values of acacetin against KB and KBv200 cells were 41.33 ± 6.05 and 49.04 ± 3.64 μM. The IC50 values of apigenin, kaempferol, kaempferol 3-O-rhamnoside, and quercetin 3-O-rhamnoside were more than 100 μM. Furthermore, quercetin was found to induce apoptosis in KB and KBv200 cells via the mitochondrial pathway, including a decrease of the reactive oxygen species level, loss of mitochondrial membrane potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly (ADP-ribose) polymerase. The apoptosis induced by quercetin was not related to the regulation of Bcl-2 or Bax in KB and KBv200 cells.
authors: School of Chinese Medicine, Hong Kong Baptist University , Kowloon Tong, Kowloon, Hong Kong.
authors: Jian-ye
authors: JY
authors: Zhang
authors: Tao
authors: T
authors: Yi
authors: Jing
authors: J
authors: Liu
authors: Zhong-zhen
authors: ZZ
authors: Zhao
authors: Hu-biao
authors: HB
authors: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000648:ultrastructure*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology*; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D045304:Cytochromes c; D011794:Quercetin