title: [Impact of high-fat diet induced obesity on glucose absorption in small intestinal mucose in rats].
abstract: OBJECTIVE
  To investigate whether high-fat diet induced obesity was associated with variation of glucose absorption in small intestinal mucosa of rats.
  
  
  METHODS
  46 male SD rats were randomly divided into high-fat diet group (n = 31) and control group (n = 15), fed with high-fat diet and normal diet for 24 weeks, respectively. After 24 weeks, the rats were divided into obese (n = 16) and obesity-resistant group (n = 10) according to their body weight. Rats' body weight, abdominal fat weight, plasma glucose level, maltase, sucrase activity in small intestinal mucosa were measured. SGLT-1 expression in intestinal mucosa was detected by immunohistochemistry, RT-PCR and Western blot.
  
  
  RESULTS
  Mean body weight, abdominal fat weight, fast plasma glucose levels, maltase activities and SGLT-1 protein expression in intestinal mucosa of obese rats were significantly higher than those in the control and obesity-resistant rats (P < 0.05). Sucrase activities in intestinal mucosa showed no statistical difference among the three groups (P > 0.05). The SGLT-1 mRNA expression in obese group was increased by 12.5% and 23% when compare with the control and obesity-resistant group, respectively. But the difference was not statistical significant (P > 0.05).
  
  
  CONCLUSIONS
  High-fat diet induced obesity was associated with the increased intestinal maltase activity and expression of SGLT-1 in rats, the key molecule in glucose absorption.
authors: Division of Peptides Related with Human Disease, West China Hospital, Sichuan University, Sichuan University, Chengdu 610041, China. cdqww@yahoo.cn
authors: Wei
authors: W
authors: Huang
authors: Rui
authors: R
authors: Liu
authors: Wei
authors: W
authors: Guo
authors: Na
authors: N
authors: Wei
authors: Ou
authors: O
authors: Qiang
authors: Xian
authors: X
authors: Li
authors: Yan
authors: Y
authors: Ou
authors: Chengwei
authors: C
authors: Tang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004041:Dietary Fats / Q000008:administration & dosage*; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics / Q000378:metabolism; D013393:Sucrase / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D012333:RNA, Messenger; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D000520:alpha-Glucosidases; D013393:Sucrase; D005947:Glucose