title: A β-glucan from the alkaline extract of a somatic hybrid (PfloVv5FB) of Pleurotus florida and Volvariella volvacea: structural characterization and study of immunoactivation.
abstract: A water soluble polysaccharide isolated from the alkaline extract of the somatic hybrid mushroom (PfloVv5FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains was found to contain d-glucose only. Structural investigation was carried out using acid hydrolysis, methylation analysis; periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC). On the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This polysaccharide exhibited strong immunoactivation of macrophages, splenocytes as well as thymocytes.
authors: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Kankan K
authors: KK
authors: Maity
authors: Sukesh
authors: S
authors: Patra
authors: Biswajit
authors: B
authors: Dey
authors: Sanjoy K
authors: SK
authors: Bhunia
authors: Soumitra
authors: S
authors: Mandal
authors: Birendra
authors: B
authors: Bahera
authors: Tapas K
authors: TK
authors: Maiti
authors: Samir R
authors: SR
authors: Sikdar
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002240:Carbohydrate Sequence; D006824:Hybridization, Genetic; D006863:Hydrogen-Ion Concentration; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000737:chemistry* / Q000235:genetics; D013154:Spleen / Q000276:immunology; D060168:Thymocytes / Q000187:drug effects / Q000276:immunology; D055367:Volvariella / Q000737:chemistry* / Q000235:genetics; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D047071:beta-Glucans