title: Loop-mediated isothermal amplification assay targeting the femA gene for rapid detection of Staphylococcus aureus from clinical and food samples.
abstract: In this study, a loop-mediated isothermal amplification (LAMP) method to rapidly detect Staphylococcus aureus strains was developed and evaluated by extensively applying a large number of S. aureus isolates from clinical and food samples. Six primers were specially designed for recognizing eight distinct sequences on the species-specific femA gene of S. aureus. The detection limits were 100 fg DNA/tube and 10(4) CFU/ml. The LAMP assay was applied to 432 S. aureus strains isolated from 118 clinical and 314 food samples. Total detection rates for the LAMP and polymerase chain reaction assays were 98.4% (306/311) and 89.4% (278/311), respectively.
authors: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, China.
authors: Xihong
authors: X
authors: Zhao
authors: Yanmei
authors: Y
authors: Li
authors: Myoungsu
authors: M
authors: Park
authors: Jun
authors: J
authors: Wang
authors: Youhong
authors: Y
authors: Zhang
authors: Xiaowei
authors: X
authors: He
authors: Fereidoun
authors: F
authors: Forghani
authors: Li
authors: L
authors: Wang
authors: Guangchao
authors: G
authors: Yu
authors: Deog-Hwan
authors: DH
authors: Oh
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005516:Food Microbiology / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; C096686:FemA protein, Bacteria