title: Isolation and characterization of 8 microsatellite  loci from Chrysophyllum gonocarpum  (Sapotaceae).
abstract: Chrysophyllum gonocarpum is a tropical tree species that is very important in  the recovery of heterogeneous forests and of degraded areas of permanent  preservation. We identified microsatellite loci for C. gonocarpum to  assess the genetic variability and the patterns of the population structure of  the species. We isolated 8 microsatellite primers by using CT- and GT-enriched  genomic libraries. We detected 2-4 alleles with 2.9 alleles per locus on  average, by polymerase chain reaction. Test for cross-amplification showed that  some loci were successfully amplified in 2 other Chrysophyllum species.  The microsatellites can be used to assess the genetic diversity and population  structure of C. gonocarpum. Some primer pairs can be amplified in C.  marginatum and C. splendens.
authors: Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors: L A
authors: LA
authors: Rodrigues
authors: E A
authors: EA
authors: Ruas
authors: N C
authors: NC
authors: Signori
authors: B F
authors: BF
authors: Costa
authors: D C L
authors: DC
authors: Farias-Missão
authors: B G
authors: BG
authors: Vieira
authors: A R O
authors: AR
authors: Conson
authors: C L
authors: CL
authors: Chaves
authors: J
authors: J
authors: Delfini
authors: C F
authors: CF
authors: Ruas
authors: P M
authors: PM
authors: Ruas
mesh_terms: D000483:Alleles; D018744:DNA, Plant / Q000235:genetics*; D014644:Genetic Variation; D015698:Genomic Library; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D029632:Sapotaceae / Q000235:genetics*
chemical_list: D018744:DNA, Plant