title: CdTe nanobioprobe based optoelectrochemical immunodetection of diabetic marker HbA1c.
abstract: Highly luminescent water soluble CdTe quantum dots (QDs) were synthesized and conjugated with anti-HbA1c antibody to generate specific nanobioprobe. A sandwich immunoassay model was employed using capture HbA1c antibody as a specific receptor molecule and the QD-labeled secondary antibody as a dual (fluorescence cum electrochemical) tracer to quantify the concentration of HbA1c. A linear increase in current was observed for HbA1c analytical standards with a R(2) value of 0.990 and coefficient of variance ~5%. A comparison between HPLC and dual immunoassay for clinical samples showed a correlation coefficient of 89% and 96% for fluorescence and electrochemical detection methods respectively. The QD-based immunoassay shows great promise for rapid reproducible and cost effective analysis of HbA1c in clinical samples.
authors: CSIR-Institute of Microbial Technology, Chandigarh 160036, India.
authors: Adity
authors: A
authors: Chopra
authors: Satish
authors: S
authors: Tuteja
authors: Naresh
authors: N
authors: Sachdeva
authors: K K
authors: KK
authors: Bhasin
authors: Vijayender
authors: V
authors: Bhalla
authors: C Raman
authors: CR
authors: Suri
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D019187:Cadmium Compounds / Q000737:chemistry*; D003920:Diabetes Mellitus / Q000175:diagnosis*; D055664:Electrochemical Techniques / Q000379:methods; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D018796:Immunoconjugates / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013691:Tellurium / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D019187:Cadmium Compounds; D006442:Glycated Hemoglobin; D018796:Immunoconjugates; D049408:Luminescent Agents; D013691:Tellurium; C517652:hemoglobin A1c protein, human; C028337:cadmium telluride