title: Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells.
abstract: Dicer, a member of the RNase III family, is the key enzyme required for the biogenesis of microRNAs and small interfering RNAs. Recent evidence indicates that DICER1 expression levels vary among different solid tumors and decreased or increased DICER1 expression has been associated with aggressive cancers. In this study, we assessed DICER1 expression levels in acute myeloid leukemia (AML) and investigated its biological effects and transcriptional regulation in leukemia cell lines. We demonstrated that DICER1 was overexpressed in AML patients and leukemia cell lines by real-time quantitative PCR and western blot analysis. A functional assay demonstrated that the silencing of DICER1 inhibited cell proliferation and promoted apoptosis in leukemia cell lines. We also demonstrated that DICER1 was upregulated by the hematopoietic transcription factor, GATA1, through luciferase, electrophoretic mobility shift and chromatin immunoprecipitation assays. These data suggest that DICER1 plays an important role in AML and the finding that the upregulation of DICER1 is induced by GATA1 may provide a framework for the understanding of differential DICER1 expression levels in multiple types of cancer.
authors: Department of Medical Genetics, China Medical University, Shenyang, P.R. China. baiying3939@126.com
authors: Ying
authors: Y
authors: Bai
authors: Guang-Rong
authors: GR
authors: Qiu
authors: Fan
authors: F
authors: Zhou
authors: Li-Ying
authors: LY
authors: Gong
authors: Feng
authors: F
authors: Gao
authors: Kai-Lai
authors: KL
authors: Sun
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D016022:Case-Control Studies; D049109:Cell Proliferation; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015973:Gene Expression Regulation, Leukemic; D055785:Gene Knockdown Techniques; D006801:Humans; D020014:K562 Cells; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D020298:U937 Cells; D015854:Up-Regulation
chemical_list: D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D034741:RNA, Small Interfering; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases