title: The role of SNAP-tag in technical approaches.
abstract: Biosensors are used for a variety of applications in medicine and biology. A critical step during the development of such devices is the coordination of biological and technical requirements. The design of the device, as well as of the sample chamber and its functionalized surface is of great importance. Depending on the surface, the method of coupling of the desired receptor has to be adapted to guarantee functionality and biological activity during the measuring process. By using the SNAP-tag technology, a site-specific coupling of molecules with unaltered activity to a variety of O(6)-benzylguanine functionalized surfaces is possible, making it a versatile tool for the setup of biomedical devices.
authors: Department of Experimental Medicine and Immunotherapy, Institute of Applied Medical Engineering, Helmholtz Institute, RWTAachen University, Pauwelsstr. 20, 52074 Aachen, Germany. stein@hia.rwth-aachen.de
authors: Christoph
authors: C
authors: Stein
authors: Christiane
authors: C
authors: Puettmann
authors: Daniel
authors: D
authors: Haamann
authors: Martin
authors: M
authors: Moeller
authors: Doris
authors: D
authors: Klee
mesh_terms: D000818:Animals; D000906:Antibodies; D015374:Biosensing Techniques / Q000295:instrumentation*; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D011485:Protein Binding
chemical_list: D000906:Antibodies; D055767:Immobilized Proteins