title: Sugarcane bagasse ozonolysis pretreatment: effect on enzymatic digestibility and inhibitory compound formation.
abstract: Sugarcane bagasse was pretreated with ozone to increase lignocellulosic material digestibility. Bagasse was ozonated in a fixed bed reactor at room temperature, and the effect of the two major parameters, ozone concentration and sample moisture, was studied. Acid insoluble and total lignin decreased whereas acid soluble lignin increased in all experiments. Pretreatment barely attacked carbohydrates, with cellulose and xylan recovery rates being >92%. Ozonolysis increased fermentable carbohydrate release considerably during enzymatic hydrolysis. Glucose and xylose yields increased from 6.64% and 2.05%, for raw bagasse, to 41.79% and 52.44% under the best experimental conditions. Only xylitol, lactic, formic and acetic acid degradation compounds were found, with neither furfural nor HMF (5-hydroxymethylfurfural) being detected. Washing detoxification provided inhibitor removal percentages above 85%, increasing glucose hydrolysis, but decreasing xylose yield by xylan solubilization. SEM analysis showed structural changes after ozonization and washing.
authors: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Valladolid, Spain. rtravaini@gmail.com
authors: Rodolfo
authors: R
authors: Travaini
authors: Marian Derly Morales
authors: MD
authors: Otero
authors: Mónica
authors: M
authors: Coca
authors: Roberto
authors: R
authors: Da-Silva
authors: Silvia
authors: S
authors: Bolado
mesh_terms: D000143:Acids / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism* / Q000648:ultrastructure; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D010126:Ozone / Q000494:pharmacology*; D031786:Saccharum / Q000737:chemistry*; D012995:Solubility; D013997:Time Factors
chemical_list: D000143:Acids; D010126:Ozone; C008046:5-hydroxymethylfurfural; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D005662:Furaldehyde; D002480:Cellulase; D005947:Glucose