title: A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae.
abstract: The physiological roles of the thioredoxin isozymes in the yeast Saccharomyces cerevisiae were investigated using a novel FRET-based redox probe, Redoxfluor. After establishing responsiveness of the probe toward thioredoxin, we followed the fluorescence signal of Redoxfluor expressed in the yeast and found that one of the thioredoxin isozymes, Trx2, was required for maintaining the redox status when stationary culture of the organism was exposed to starvation and mild-heat stresses. The failure to maintain redox balance under the tested condition preceded decreased viability of the trx2 mutants, indicating the functional importance of the cytoplasmic thioredoxin in adaptation to environmental changes.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo, Kyoto 606-8502, Japan.
authors: Masahide
authors: M
authors: Oku
authors: Jun
authors: J
authors: Hoseki
authors: Yayoi
authors: Y
authors: Ichiki
authors: Yasuyoshi
authors: Y
authors: Sakai
mesh_terms: D015374:Biosensing Techniques; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013217:Starvation; D013312:Stress, Physiological; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D007527:Isoenzymes; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C516110:TRX2 protein, S cerevisiae; D013879:Thioredoxins