title: Lymphatic diamine oxidase secretion stimulated by fat absorption is linked with histamine release.
abstract: Diamine oxidase (DAO) is abundantly expressed in mammalian small intestine catalyzing the oxidative breakdown of polyamines and histamine. The aim of this study was to determine the relationship between stimulation of intestinal diamine oxidase secretion with intestinal fat absorption and histamine release. Conscious intestinal lymph fistula rats were used. The mesenteric lymph ducts were cannulated and intraduodenal tubes were installed for the infusion of Liposyn II 20% (an intralipid emulsion). Lymphatic DAO activity and protein secretion were analyzed by radiometric assay and Western blot, respectively. Lymphatic histamine concentration was measured by ELISA. Infusion of Liposyn II (4.43 kcal/3 ml) resulted in a ~3.5-fold increase in lymphatic DAO protein secretion and DAO activity, peaking at 1 h and lasting for 3 h. Liposyn II infusion also increased the lymphatic histamine release, a substrate for DAO. To determine the relationship of DAO release with histamine release, histamine was administered intraperitoneally (10 mg/kg) in fasting rats and resulted in a significant doubling in lymphatic DAO activity, supporting a link between histamine and DAO. In addition, ip administration of the histamine H4 receptor antagonist JNJ7777120 significantly reduced the Liposyn II-induced DAO output by 65.9%, whereas H(1) (pyrilamine maleate), H(2) (ranitidine), and H(3) (thioperamide maleate) receptor antagonists had little effect. We conclude that DAO secretion may contribute to the catabolism of histamine released during fat absorption and this is probably mediated through the histamine H(4) receptor.
authors: Department of Pathology and Laboratory Medicine, Metabolic Diseases Institute, University of Cincinnati, Cincinnati, OH, USA.
authors: Yong
authors: Y
authors: Ji
authors: Yasuhisa
authors: Y
authors: Sakata
authors: Xiaoming
authors: X
authors: Li
authors: Chao
authors: C
authors: Zhang
authors: Qing
authors: Q
authors: Yang
authors: Min
authors: M
authors: Xu
authors: Armin
authors: A
authors: Wollin
authors: Wolfgang
authors: W
authors: Langhans
authors: Patrick
authors: P
authors: Tso
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D004195:Disease Models, Animal; D004386:Duodenum / Q000378:metabolism / Q000473:pathology; D004655:Emulsions / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism*; D006633:Histamine Antagonists / Q000494:pharmacology; D007412:Intestinal Fistula / Q000378:metabolism* / Q000473:pathology; D008208:Lymphatic System / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D008297:Male; D010743:Phospholipids / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D011968:Receptors, Histamine / Q000378:metabolism*; D000074040:Receptors, Histamine H4; D012450:Safflower Oil / Q000494:pharmacology; D013024:Soybean Oil / Q000494:pharmacology
chemical_list: D004041:Dietary Fats; D004655:Emulsions; D005217:Fat Emulsions, Intravenous; D006633:Histamine Antagonists; C498828:Hrh4 protein, rat; D010743:Phospholipids; D043562:Receptors, G-Protein-Coupled; D011968:Receptors, Histamine; D000074040:Receptors, Histamine H4; C063336:safflower oil, soybean oil, phospholipid emulsion; D013024:Soybean Oil; D012450:Safflower Oil; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing)