title: The molecular background of the differential UV absorbance of the human lens in the 240-400 nm range.
abstract: The ultraviolet (UV) absorption of various sections of the human lens was studied and compared with protein expression paralleling differential UV absorbance in anterior and posterior lenticular tissue. The UV absorbance of serial lens cryostat sections (60 μm) and that of lens capsules was determined using a Shimadzu scanning spectrophotometer, and the absorption coefficients were calculated. Two-dimensional gel electrophoresis was performed using two pooled lenticular protein extracts (anterior and posterior sections). Protein spots were quantified and significantly different spots were identified by mass spectrometry following in-gel digestion with trypsin and chymotrypsin. The UV-C and UV-B absorption of the human lens increased toward the posterior parts of the lens. The anterior and posterior lens capsules also effectively absorbed UV radiation. Levels of molecular chaperone proteins Beta-crystallin B2 (UniProtKB ID:P43320), A3 (UniProtKB ID:P05813) and of glyceraldehyde 3-phosphate dehydrogenase (UniProtKB ID:P04406) were significantly higher in the anterior part of the lens, whereas lens proteins Beta-crystallin B1 (UniProtKB ID:P53674) and Alpha-crystallin A chain (UniProtKB ID:P02489) were higher in the posterior sections. These results provide evidence that differential UV absorption in the anterior and posterior lens is accompanied by differential protein expression.
authors: Department of Ophthalmology, Albert Szent-Györgyi Clinical Centre, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors: Viktor
authors: V
authors: Pajer
authors: Akos
authors: A
authors: Tiboldi
authors: Narkhyun
authors: N
authors: Bae
authors: Kongzhao
authors: K
authors: Li
authors: Sung Ung
authors: SU
authors: Kang
authors: Béla
authors: B
authors: Hopp
authors: Lajos
authors: L
authors: Kolozsvári
authors: Gert
authors: G
authors: Lubec
authors: Antal
authors: A
authors: Nógrádi
mesh_terms: D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry*; D008875:Middle Aged; D013054:Spectrophotometry, Atomic; D014466:Ultraviolet Rays