title: Altered melatonin secretion and circadian gene expression with increased proinflammatory cytokine expression in early-stage sepsis patients.
abstract: Inflammatory and immune responses, as well as melatonin secretion, are affected by circadian regulation. Abnormal circadian rhythm of melatonin release has been reported to be associated with the later stages of sepsis; however, its role in the early stages of sepsis is unclear. We studied 11 septic and 11 non-septic patients in our intensive care unit (ICU). Peripheral blood was drawn at 4-h intervals on the first day, beginning at 2:00 p.m., over a total period of 24 h. Plasma levels of melatonin, tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) were measured by radioimmunoassay or enzyme-linked immunosorbent assay (ELISA). Messenger RNA levels of circadian genes Cry-1 and Per-2 were analyzed using quantitative real-time PCR. Results show the circadian rhythm of melatonin secretion was altered in the early stages of sepsis. The melatonin secretion acrophase occurred earlier in septic patients at 6:00 p.m., compared with at 2:00 a.m. in non-septic ICU patients. Compared with the non-septic group, both Cry-1 and Per-2 expression were significantly decreased while TNF-α and IL-6 expression were significantly increased in septic patients [TNF-α, 64.1 (43.6-89.1) vs. 11.4 (10.4-12.5) ng/ml; IL-6, 41.2 (35.7-50.8) vs. 19.1 (16-136.7) ng/ml; median (range), both P=0.04]. The peak concentrations of TNF-α and IL-6 were shown to be in concordance with the rhythm of melatonin secretion. The circadian rhythm of melatonin secretion and circadian gene expression were altered in the early stages of sepsis, which likely led to the changes in pro-inflammatory cytokine release. These findings shed light on the potential link between circadian rhythm and the progression of early-stage sepsis.
authors: Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, P.R. China.
authors: Cai-Xia
authors: CX
authors: Li
authors: Dong-Dong
authors: DD
authors: Liang
authors: Guo-Hao
authors: GH
authors: Xie
authors: Bao-Li
authors: BL
authors: Cheng
authors: Qi-Xing
authors: QX
authors: Chen
authors: Shui-Jing
authors: SJ
authors: Wu
authors: Jun-Lu
authors: JL
authors: Wang
authors: William
authors: W
authors: Cho
authors: Xiang-Ming
authors: XM
authors: Fang
mesh_terms: D000328:Adult; D000368:Aged; D002940:Circadian Rhythm / Q000235:genetics*; D056931:Cryptochromes / Q000096:biosynthesis; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D008550:Melatonin / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D056950:Period Circadian Proteins / Q000096:biosynthesis; D018805:Sepsis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: C538963:CRY1 protein, human; D056931:Cryptochromes; D015850:Interleukin-6; C421902:PER2 protein, human; D056950:Period Circadian Proteins; D014409:Tumor Necrosis Factor-alpha; D008550:Melatonin