title: The role of angiogenin in pT1-T2 tongue carcinoma neo-angiogenesis and cell proliferation: an exploratory study.
abstract: BACKGROUND
  Angiogenin (ANG) is a member of the ribonuclease superfamily and of medical interest largely because it supports the growth of primary and metastatic malignancies. This study is the first to investigate the potential role of ANG in tongue carcinoma neo-angiogenesis and cancer cell proliferation.
  
  
  METHODS
  Angiogenin expression (in carcinoma cells and endothelial intratumor vessel cells), CD105-assessed micro-vessel density (MVD), and MIB-1 expression were correlated with prognostic parameters in 28 primarily consecutively operated pT1-T2 tongue carcinomas (squamous cell carcinoma [SCC]). Whenever feasible, a computer-based image analysis system was used for the immunohistochemical reaction analysis.
  
  
  RESULTS
  No significant correlations emerged between ANG expression in the tongue carcinoma cells or endothelial intratumor vessel cells and tongue SCC recurrence rate or disease-free survival (DFS). ANG expression was also unrelated to CD105-assessed MVD or MIB-1 expression. Conversely, CD105-assessed MVD correlated directly with recurrence rate (P = 0.02) and DFS was significantly shorter in cases with CD105-assessed MVD >167 micro-vessels/mm(2) than in those with CD105-assessed MVD ≤167 micro-vessels/mm(2) (P = 0.042).
  
  
  CONCLUSIONS
  Our results support the hypothesis that CD105-assessed MVD would be a valuable parameter for predicting which patients with tongue SCC are at greatest risk of disease recurrence. Despite our study results, the role of ANG in tongue carcinoma warrants further investigation in larger series.
authors: Department of Neurosciences, Otolaryngology Section, University of Padova, Padova, Italy.
authors: Gino
authors: G
authors: Marioni
authors: Alberto
authors: A
authors: Staffieri
authors: Elena
authors: E
authors: Fasanaro
authors: Roberto
authors: R
authors: Stramare
authors: Luciano
authors: L
authors: Giacomelli
authors: Elena
authors: E
authors: Bernardi
authors: Matteo
authors: M
authors: Val
authors: Edoardo
authors: E
authors: Stellini
authors: Cosimo
authors: C
authors: de Filippis
authors: Stella
authors: S
authors: Blandamura
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000032:analysis / Q000494:pharmacology*; D015703:Antigens, CD / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000098:blood supply*; D049109:Cell Proliferation / Q000187:drug effects; D018572:Disease-Free Survival; D000071063:Endoglin; D042783:Endothelial Cells / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D005500:Follow-Up Studies; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D019394:Ki-67 Antigen / Q000032:analysis; D055806:Microvessels / Q000473:pathology; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000032:analysis; D012259:Ribonuclease, Pancreatic / Q000032:analysis / Q000502:physiology*; D014062:Tongue Neoplasms / Q000098:blood supply*; D016896:Treatment Outcome
chemical_list: D043925:Angiogenesis Inducing Agents; D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D019394:Ki-67 Antigen; D011956:Receptors, Cell Surface; C046366:angiogenin; D012259:Ribonuclease, Pancreatic
keywords: CD105; MIB-1; angiogenin; prognosis; tongue carcinoma