title: Augmentation of PCR efficiency using highly thermostable gold nanoparticles synthesized from a thermophilic bacterium, Geobacillus stearothermophilus.
abstract: Biogenic gold nanoparticles were synthesized from the Geobacillus stearothermophilus cell-free extract. These nanoparticles were characterized by UV-vis spectroscopy, FTIR, TEM, and XRD. The gold nanoparticles showed an absorption maximum at 522 nm. The TEM micrograph revealed the formation of monodispersed particles. The high degree of stability of the nanoparticle solution could be attributed to the secretion of certain capping proteins by the bacterium in the reaction mixture which was confirmed by the FTIR and UV-vis spectrometric analyses. The heat transfer property of the gold nanoparticles in aqueous solution has been explored in the current study for augmenting the PCR efficiency. The highly thermostable biogenic gold nanoparticles effectively increased the yield, product specificity besides reducing the reaction time of the PCR.
authors: CAS in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India. girilal@gmail.com
authors: M
authors: M
authors: Girilal
authors: A
authors: A
authors: Mohammed Fayaz
authors: P
authors: P
authors: Mohan Balaji
authors: P T
authors: PT
authors: Kalaichelvan
mesh_terms: D001411:Geobacillus stearothermophilus / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance; D014961:X-Ray Diffraction
chemical_list: D006046:Gold