title: Activatable rotor for quantifying lysosomal viscosity in living cells.
abstract: We have developed Lyso-V, the first fluorescent probe of lysosomal viscosity. Because of its lysosome-actived fluorescence characteristics, Lyso-V has proved to be an ideal lysosomal tracer with high spatial and temporal resolution under laser confocal microscopy. More importantly, Lyso-V shows its practical applicability in real-time quantification of lysosomal viscosity changes in live cells through fluorescence lifetime imaging microscopy.
authors: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
authors: Lu
authors: L
authors: Wang
authors: Yi
authors: Y
authors: Xiao
authors: Wenming
authors: W
authors: Tian
authors: Liezheng
authors: L
authors: Deng
mesh_terms: D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D061986:MCF-7 Cells; D013050:Spectrometry, Fluorescence; D014783:Viscosity