title: Multiphoton microscopic imaging of esophagus during the early phase of tumor progression.
abstract: Esophageal cancer is one of the most common cancer and leading cause of cancer death worldwide. Multiphoton microscopy (MPM) has become a novel optical tool of choice for imaging tissue architecture and cellular morphology based on two-photon excited fluorescence and second harmonic generation. In this study, we used MPM to image microstructure of human normal esophagus, carcinoma in situ, and early invasive carcinoma in order to investigate the morphological change of tissue structure during the early phase of tumor progression. The diagnostic features such as the appearance of cancerous cells, the absence of the basement membrane were extracted to distinguish between normal and cancerous esophagus tissue. The infiltration depth during tumor progression was determined by the appearance of cancerous cells. The significant change of layer structure between cancerous tissue and normal esophagus was described. We also quantitatively described the differences of morphology between normal and cancerous cells. These results correlated well with the corresponding histological findings. With the advancement of clinically miniaturized MPM and the multi-photon probe, combining MPM with standard endoscopy will therefore allow us to make a real-time in vivo diagnosis of early esophageal cancer at the cellular level.
authors: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
authors: Jian
authors: J
authors: Xu
authors: Deyong
authors: D
authors: Kang
authors: Meifang
authors: M
authors: Xu
authors: Shuangmu
authors: S
authors: Zhuo
authors: Xiaoqin
authors: X
authors: Zhu
authors: Jianxin
authors: J
authors: Chen
mesh_terms: D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D004947:Esophagus / Q000473:pathology*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*
keywords: basement membrane (BM); cancerous cell; depth of infiltration; early phase of esophageal tumor; multiphoton microscopy (MPM)