title: Evaluation of biodegradable peptide carriers for light-directed targeting.
abstract: A promising strategy for increased intracellular delivery of nucleic acids with the benefit for targeting is photochemical internalization (PCI). PCI relies on the use of a photosensitizing compound that photochemically destroys membranes in the endocytic pathway after illumination, resulting in cytosolic transfer of endosomal content. PCI technology combined with biodegradable polyamino acid carriers and nucleic acids delivers effective targeting and improved biosafety. In an in vitro model system, we have evaluated various poly-l-lysine (PLL), poly-l-histidine (PLH), and poly-l-arginine (PLA) formulations for light-directed small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. We find that PLA formulations are suitable as siRNA and mRNA carriers in a strictly light-directed manner.
authors: Department of Tumor Biology, Institute for Cancer Research , The Norwegian Radium Hospital, Oslo, Norway .
authors: Jens Andreas Lindin
authors: JA
authors: Jørgensen
authors: Ane Sager
authors: AS
authors: Longva
authors: Eivind
authors: E
authors: Hovig
authors: Sigurd Leinæs
authors: SL
authors: Bøe
mesh_terms: D001120:Arginine / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism; D015316:Genetic Therapy; D006801:Humans; D008027:Light; D008239:Lysine / Q000008:administration & dosage / Q000737:chemistry; D008545:Melanoma / Q000188:drug therapy / Q000235:genetics; D058990:Molecular Targeted Therapy; D009696:Nucleic Acids / Q000008:administration & dosage / Q000737:chemistry; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry; D014162:Transfection
chemical_list: D009696:Nucleic Acids; D010455:Peptides; D017319:Photosensitizing Agents; D034741:RNA, Small Interfering; D001120:Arginine; D008239:Lysine