title: Microarray analysis of differentially expressed transcripts in porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus as a model to study innate immune responses to enteric viruses.
abstract: The local intestinal mucosa, the largest mucosal immune system in animals, plays an important role in resistance against intestinal pathogen infection. However, the molecular antiviral mechanisms of the intestinal mucosa remain poorly understood. In this study, we screened and identified differentially expressed transcripts in (PSV) porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus using microarray analysis. A total of 2298 differentially expressed genes were screened at four time points during PSV infection. These genes were involved in numerous physical systems and molecular pathways, and particularly, some innate immune-associated pathways were significant. The results showed that large amounts of type I interferon were induced, and the related interferon effect pathway was activated when IPEC-J2 cells were infected with PSV. Three pathways of innate immune receptors, including Toll-like, NOD-like, and RIG-I-like receptors, were also activated. The antigen was then processed and presented through the MHCI and MHCII pathways. Interestingly, we found that the secretion network of IgA was activated in the early stage of PSV infection. Two exogenous and endogenous apoptosis pathways were also activated during PSV infection. The results revealed changes in gene transcription, particularly those of innate immune pathway genes that were associated with PSV infection in IPEC-J2 cells.
authors: Hi-tech Research and Development Base for Qinghai-Tibet Plateau Ecological Conservation and Stock Farming, Southwest University for Nationality, Chengdu, China.
authors: Daoliang
authors: D
authors: Lan
authors: Cheng
authors: C
authors: Tang
authors: Hua
authors: H
authors: Yue
authors: Huan
authors: H
authors: Sun
authors: Li
authors: L
authors: Cui
authors: Xiuguo
authors: X
authors: Hua
authors: Jian
authors: J
authors: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D046228:Microarray Analysis; D010849:Picornaviridae / Q000276:immunology* / Q000472:pathogenicity*; D015398:Signal Transduction; D013552:Swine; D013997:Time Factors