title: Activation profile of CXCL8-stimulated neutrophils and aging.
abstract: Neutrophils are pivotal effector cells of innate immunity representing the first line of defense against aggression. They are the first cells to arrive at the site of the aggression, where they can directly eliminate the invading microorganisms. Their activation and recruitment into peripheral tissues is indispensable for host defense. With aging, there are alterations of the receptor by driven functions of human neutrophils as a decrease in the functional changes in signaling elicited by specific receptors, as CXCR1. We investigated the activation of neutrophils from elderly after the cells were cultivated with CXCL8. Although, CXCL8 induced elastase (ELA) secretion, data showed neither myeloperoxidase (MPO) activity nor production of IL-6, IL-10, GM-CSF by neutrophils from elderly compared with young individuals. On the other hand, in the presence of only LPS or LPS associated with CXCL8 neutrophils from elderly individuals, there were significant levels of IL-6, IL-10, GM-CSF but not MPO. These results indicate that neutrophils from elderly do not respond to CXCL8 stimulus, but they are activated by LPS to produce cytokines. However, MPO activity from elderly individuals was not different in the presence or absence of LPS and CXCL8.
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors: Thalita Marcato
authors: TM
authors: Dalboni
authors: Aneli Eiko
authors: AE
authors: Abe
authors: Carine Ervolino
authors: CE
authors: de Oliveira
authors: Vanessa Soares
authors: VS
authors: Lara
authors: Ana Paula
authors: AP
authors: Campanelli
authors: Carlos Teodoro
authors: CT
authors: Gasparoto
authors: Thaís Helena
authors: TH
authors: Gasparoto
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D008875:Middle Aged; D018375:Neutrophil Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D010196:Pancreatic Elastase / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D055815:Young Adult
chemical_list: D016209:Interleukin-8; D009195:Peroxidase; D010196:Pancreatic Elastase