title: Ce(III)-induced rice mitochondrial permeability transition investigated by spectroscopic and microscopic studies.
abstract: Cerium has been widely used as fertilizer and feed additives in agriculture, but it might finally impair human health by food chain accumulation with its dosage increased in environmental and crops samples. To resolve the conflict, we investigated the effects of Ce(III) on isolated rice mitochondrial permeability transition (MPT) by examining mitochondrial swelling, transmembrane potential, membrane fluidity with spectroscopy, and observing the mitochondrial ultrastructure, meanwhile, the interaction site(s) and mechanism between Ce(III) and mitochondria were also studied. The results showed that the low level of Ce(III) had little effect on rice MPT, however, the higher level of Ce(III) could induce rice MPT, and the thiol (-SH) groups of membrane proteins (defined as "S" site) matched by Ce(III)-triggered rice MPT pore opening.
authors: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors: Cai-Fen
authors: CF
authors: Xia
authors: Jie
authors: J
authors: Zhao
authors: Jian-Cheng
authors: JC
authors: Jin
authors: Lian
authors: L
authors: Yuan
authors: Xin-You
authors: XY
authors: Chen
authors: Wu
authors: W
authors: Peng
authors: Feng-Lei
authors: FL
authors: Jiang
authors: Cai-Qin
authors: CQ
authors: Qin
authors: Jie
authors: J
authors: Dai
authors: Yi
authors: Y
authors: Liu
mesh_terms: D002563:Cerium / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D008933:Mitochondrial Swelling / Q000187:drug effects; D012275:Oryza / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D002563:Cerium