title: Bacterial contamination in ascitic fluid is associated with the development of clinically relevant pancreatic fistula after pancreatoduodenectomy.
abstract: OBJECTIVE
  Clinically relevant postoperative pancreatic fistula (POPF) after pancreatoduodenectomy is often accompanied by bacterial infection. To elucidate the mechanism of bacterial infection associated with POPF, we investigated the relationship between POPF and bacteria isolated from ascitic fluid and removed drains.
  
  
  METHODS
  Subjects were 101 patients who had undergone pancreatoduodenectomy. Microbial culture was performed using ascitic fluid obtained from drains. We also compared the isolated bacteria from removed drains on postoperative day (POD) 4 and after POD 7.
  
  
  RESULTS
  In 23 patients (22.8%), microbial cultures were already positive on POD 1, although purulent discharge was not observed. Among patients with grade B/C POPF, bacteria were detected on POD 1 in 53.8%; these isolated bacteria were the same as those isolated after POPF formation. In contrast, only 7.7% of patients with grade A POPF were positive on POD 1. The number of bacteria isolated from drains removed after POD 7 significantly increased compared with those isolated from drains removed on POD 4.
  
  
  CONCLUSIONS
  Bacterial contamination in ascitic fluid may be an initiating event that leads to the development of clinically relevant POPF. Therefore, it is important to perform both the administration of the appropriate antibiotics and early drain removal.
authors: Department of Surgery, Tokyo Medical University, Tokyo, Japan. naga@tokyo-med.ac.jp
authors: Yuichi
authors: Y
authors: Nagakawa
authors: Takaaki
authors: T
authors: Matsudo
authors: Yousuke
authors: Y
authors: Hijikata
authors: Satoru
authors: S
authors: Kikuchi
authors: Kyo
authors: K
authors: Bunso
authors: Yoshiaki
authors: Y
authors: Suzuki
authors: Kazuhiko
authors: K
authors: Kasuya
authors: Akihiko
authors: A
authors: Tsuchida
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001202:Ascitic Fluid / Q000382:microbiology*; D001424:Bacterial Infections / Q000209:etiology* / Q000382:microbiology*; D002761:Cholangitis / Q000150:complications; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010185:Pancreatic Fistula / Q000209:etiology* / Q000382:microbiology*; D010190:Pancreatic Neoplasms / Q000601:surgery; D016577:Pancreaticoduodenectomy / Q000009:adverse effects*; D011183:Postoperative Complications / Q000209:etiology* / Q000382:microbiology*; D012307:Risk Factors; D013997:Time Factors