title: A sensor tip based on carbon nanotube-ink printed electrode for the dengue virus NS1 protein.
abstract: An immunosensor for the non-structural protein 1 (NS1) of the dengue virus based on carbon nanotube-screen printed electrodes (CNT-SPE) was successfully developed. A homogeneous mixture containing carboxylated carbon nanotubes was dispersed in carbon ink to prepare a screen printed working electrode. Anti-NS1 antibodies were covalently linked to CNT-SPE by an ethylenediamine film strategy. Amperometrical responses were generated at -0.5 V vs. Ag/AgCl by hydrogen peroxide reaction with peroxidase (HRP) conjugated to the anti-NS1. An excellent detection limit (in the order of 12 ng mL(-1)) and a sensitivity of 85.59 μA mM(-1)cm(-2) were achieved permitting dengue diagnostic according to the clinical range required. The matrix effect, as well as the performance of the assays, was successfully evaluated using spiked blood serum sample obtaining excellent recovery values in the results. Carbon nanotubes incorporated to the carbon ink improved the reproducibility and sensitivity of the CNT-SPE immunosensor. This point-of-care approach represents a great potential value for use in epidemic situations and can facilitate the early screening of patients in acute phase of dengue virus.
authors: Biomedical Engineering Laboratory, Federal University of Pernambuco, Campus da Saúde, Recife, Brazil.
authors: Ana Carolina M S
authors: AC
authors: Dias
authors: Sérgio L R
authors: SL
authors: Gomes-Filho
authors: Mízia M S
authors: MM
authors: Silva
authors: Rosa F
authors: RF
authors: Dutra
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D002244:Carbon / Q000737:chemistry*; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000302:isolation & purification*; D004566:Electrodes; D004867:Equipment Design; D005029:Ethylenediamines / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000097:blood
chemical_list: D055910:Antibodies, Immobilized; D005029:Ethylenediamines; D037742:Nanotubes, Carbon; D017361:Viral Nonstructural Proteins; C031234:ethylenediamine; D002244:Carbon