title: Analysis of the K+ current in human CD4+ T lymphocytes in hypercholesterolemic state.
abstract: Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca(2+)-dependent activation pathway. In the present experiments we studied the proliferation capacity and functional changes of Kv1.3 channels in T cells from healthy and hypercholestaeremic patients. By means of CFSE-assay (carboxyfluorescein succinimidyl ester) we showed that spontaneous activation rate of lymphocytes in hypercholesterolemia was elevated and the antiCD3/antiCD28 co-stimulation was less effective as compared to the healthy group. Using whole-cell patch-clamping we obtained that the activation and deactivation kinetics of Kv1.3 channels were faster in hypercholesterolemic state but no change in other parameters of Kv1.3 were found (inactivation kinetics, steady-state activation, expression level). We suppose that incorporation of oxLDL species via its raft-rupturing effect can modify proliferative rate of T cells as well as the gating of Kv1.3 channels.
authors: 1st Department of Internal Medicine, University of Debrecen, Medical and Health Science Center, Debrecen, Hungary.
authors: Sándor
authors: S
authors: Somodi
authors: András
authors: A
authors: Balajthy
authors: Orsolya
authors: O
authors: Szilágyi
authors: Zoltán
authors: Z
authors: Pethő
authors: Mariann
authors: M
authors: Harangi
authors: György
authors: G
authors: Paragh
authors: György
authors: G
authors: Panyi
authors: Péter
authors: P
authors: Hajdu
mesh_terms: D000328:Adult; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D005452:Fluoresceins; D006801:Humans; D006937:Hypercholesterolemia / Q000276:immunology / Q000378:metabolism*; D015640:Ion Channel Gating / Q000502:physiology; D051664:Kv1.3 Potassium Channel / Q000096:biosynthesis / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008875:Middle Aged; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism*; D013388:Succinimides
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D018106:CD28 Antigens; D017252:CD3 Complex; D005452:Fluoresceins; D051664:Kv1.3 Potassium Channel; D013388:Succinimides; D011188:Potassium; D002118:Calcium