title: Haloferax mediterranei GlnK proteins are post-translationally modified by uridylylation.
abstract: In this work we report for the first time a post-translational modification of PII homologues from the Archaea Domain. Haloferax mediterranei is the first haloarchaea whose PII proteins have been studied, it possesses two of them (GlnK1 and GlnK2 ), both encoded adjacent to a gene for the ammonia transporter Amt. An approach based on 2DE, anti-GlnK immunoblot and peptide mass fingerprint (MALDI-TOF-MS) of the reactive spots showed that GlnK proteins in H. mediterranei are post-translationally uridylylated. A third spot with lower pI suggests the existence of a non-descript post-translational modification in this protein family.
authors: Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain.
authors: Laia
authors: L
authors: Pedro-Roig
authors: Mónica
authors: M
authors: Camacho
authors: María José
authors: MJ
authors: Bonete
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000032:analysis / Q000378:metabolism*; D019623:Haloferax mediterranei / Q000378:metabolism*; D015151:Immunoblotting / Q000379:methods; D008969:Molecular Sequence Data; D051922:PII Nitrogen Regulatory Proteins / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D019843:Archaeal Proteins; D051922:PII Nitrogen Regulatory Proteins