title: Multi-class method for determination of veterinary drug residues and other contaminants in infant formula by ultra performance liquid chromatography-tandem mass spectrometry.
abstract: A rapid, simple and generic analytical method which was able to simultaneously determine 220 undesirable chemical residues in infant formula had been developed. The method comprised of extraction with acetonitrile, clean-up by low temperature and water precipitation, and analysis by ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS-MS) using multiple reaction monitoring (MRM) mode. Most fat materials in acetonitrile extract were eliminated by low temperature clean-up. The water precipitation, providing a necessary and supplementary cleanup, could avoid losses of hydrophobic analytes (avermectins, ionophores). Average recoveries for spiked infant formula were in the range from 57% to 147% with associated RSD values between 1% and 28%. For over 80% of the analytes, the recoveries were between 70% and 120% with RSD values in the range of 1-15%. The limits of quantification (LOQs) were from 0.01 to 5 μg/kg, which were usually sufficient to verify the compliance of products with legal tolerances. Application of this method in routine monitoring programs would imply a drastic reduction of both effort and time.
authors: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
authors: Jia
authors: J
authors: Zhan
authors: Ying-ying
authors: YY
authors: Zhong
authors: Xue-jun
authors: XJ
authors: Yu
authors: Jin-feng
authors: JF
authors: Peng
authors: Shubing
authors: S
authors: Chen
authors: Ju-yi
authors: JY
authors: Yin
authors: Jia-Jie
authors: JJ
authors: Zhang
authors: Yan
authors: Y
authors: Zhu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D041943:Infant Formula / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D019155:Veterinary Drugs