title: [Design and evaluation of DAVIH VIH-2].
abstract: The results of the design and evaluation of DAVIH VIH-2 diagnosing system, an indirect Elisa for screening of HIV-2 antibodies, which uses a HIV-2 glycoprotein gp36 synthetic peptide in its solid phase, were exposed. In the system evaluation using WHO reference panels, 100% sensitivity, 99,81% specificity, 99,81% efficacy and very good concordance level (kappa = 0.978) were attained. Serum samples of 959 individuals with undetermined or negative results to the HIV-1 antibodies confirmation (DAVIH blot) were evaluated by the DAVIH VIH-2 system. Twenty four samples were reactive, six of which had confirmed HIV-2 antibodies. These results allowed recommending the introduction of this diagnostic kit in the HIV infection diagnosing algorithm in Cuba.
authors: Laboratorio de Investigaciones del Sida (LISIDA), La Habana, Cuba.. cicdc@infomed.sld.cu
authors: Dayamí
authors: D
authors: Martín Alfonso
authors: Eladio
authors: E
authors: Silva Cabrera
authors: María T
authors: MT
authors: Pérez Guevara
authors: Dervel F
authors: DF
authors: Díaz Herrera
authors: Kenia
authors: K
authors: Romero Martínez
authors: Héctor M
authors: HM
authors: Díaz Torres
authors: Ana L
authors: AL
authors: Lubián Caballero
authors: Nancy
authors: N
authors: Ruiz Gutiérrez
authors: Eva
authors: E
authors: Ortiz Losada
mesh_terms: D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000097:blood; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D010446:Peptide Fragments