title: A folding-based approach for the luminescent detection of a short RNA hairpin.
abstract: We report the rational design of a 20-mer basic peptide, derived from the transcriptional antitermination protein N of bacteriophage P22, equipped with a luminescent DOTA[Tb(3+)] macrocyclic complex and a sensitizing tryptophan antenna. Folding of this peptide into an α helical conformation, which occurs upon binding to its target boxB RNA hairpin, results in a large increase in luminescence emission. Therefore, the peptide construct works as a highly sensitive and selective probe for this RNA hairpin.
authors: Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares, Departamento de Química Orgánica and Unidad Asociada al CSIC, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors: Cristina
authors: C
authors: Penas
authors: Elena
authors: E
authors: Pazos
authors: José L
authors: JL
authors: Mascareñas
authors: M Eugenio
authors: ME
authors: Vázquez
mesh_terms: D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D049449:Luminescence; D008163:Luminescent Measurements; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D017510:Protein Folding; D034741:RNA, Small Interfering / Q000032:analysis*; D013725:Terbium / Q000737:chemistry*
chemical_list: D006573:Heterocyclic Compounds, 1-Ring; D010455:Peptides; D034741:RNA, Small Interfering; D013725:Terbium; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid