title: Detection of ionic liquid stable cellulase produced by the marine bacterium Pseudoalteromonas sp. isolated from brown alga Sargassum polycystum C. Agardh.
abstract: An extracellular cellulase produced by marine bacterium Pseudoalteromonas sp. was studied for its activity and stability in six different ionic liquids (ILs) over a wide range of concentrations (1-20% v/v) and compared with aqueous medium as control. Enzyme showed its optimal activity at 45°C and at pH 5 in control. Although the activity varied with the type of IL and its concentration used, the activity measured at 5% (v/v) was maximum with [EMIM]Br followed by [EMIM]Ac, [BMIM]Cl, [C2MIM][CH3SO3], [BMIM][OTF] and [BMPL][OTF] with 115%, 104.7%, 102.2%, 98.33%, 93.84% and 92.67%, respectively, and >80% activity at 15% (v/v) in all ILs. The enzyme stability at 5% (v/v) IL concentration for 36h was superior to commercial cellulase. The cellulase activity enhanced by 1.35- to 1.72-fold over control when 5% (v/v) IL based reaction medium with algal biomass was used and thus showed potentials for saccharification of biomass in a single step process.
authors: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors: Nitin
authors: N
authors: Trivedi
authors: Vishal
authors: V
authors: Gupta
authors: C R K
authors: CR
authors: Reddy
authors: Bhavanath
authors: B
authors: Jha
mesh_terms: D018533:Biomass; D002384:Catalysis; D002480:Cellulase / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000378:metabolism; D031161:Pseudoalteromonas / Q000201:enzymology* / Q000235:genetics; D044647:Sargassum / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004275:DNA, Ribosomal; D052578:Ionic Liquids; D002480:Cellulase