title: [Coal tar pitch smoke extract-induced pyroptosis in human bronchial epithelial cells].
abstract: OBJECTIVE
  To explore whether coal tar pitch smoke extract (CTP) induced pyroptosis in human bronchial epithelial cells (BEAS-2B).
  
  
  METHODS
  BEAS-2B cells were treated with different concentrations of CTP (1, 3 µg/ml) for 8h and 24 h, respectively. Lactic dehydrogenase (LDH) activity and interleukin-1 beta (IL-1β) levels in the supernatants of cell culture media were measured with LDH activity or human IL-1β ELISA kit, respectively. The activity of Caspase-1 was measured with Caspase-1 colorimetric assay kit.
  
  
  RESULTS
  The activity of caspase-1 in 1 and 3 µg/ml CTP groups were (9.29 ± 0.30) and (8.67 ± 0.59) µmol/ml respectively which were both significantly increased compared to that (7.42 ± 0.59) µmol/ml in the control group (P < 0.05) after 8 h exposure, but there was no significant difference in the activity of LDH and levels of IL-1β in the cell culture media among the CTP and control groups. 24 h after exposure, the activity of LDH in the CTP (1, 3 µg/ml) groups were (1323.03 ± 28.53) and (1148.45 ± 16.42) U/dl respectively which were significantly higher than that (1091.93 ± 26.64) U/dl in the control group (P < 0.05), and the levels of IL-1β in the CTP (1 and 3 µg/ml) groups were (125.37 ± 25.00) pg/ml and (92.04 ± 19.09) pg/ml respectively which were significantly higher than that (46.20 ± 14.43) pg/ml in the control group (P < 0.05), but there was no significant difference in the activity of Caspase-1 among CTP and control groups (P < 0.05).
  
  
  CONCLUSIONS
  CTP treatment induced early increase in caspase-1 activity followed by the increase in LDH activity and IL-1 levels, indicative of pyroptosis in human bronchial epithelial cells.
authors: Department of Occupational Health, Zhengzhou University, Zhengzhou, China.
authors: Jin-yan
authors: JY
authors: Song
authors: Ya-nan
authors: YN
authors: Feng
authors: Li-peng
authors: LP
authors: Du
authors: Wu
authors: W
authors: Yao
authors: Yi-ming
authors: YM
authors: Wu
authors: Wei-dong
authors: WD
authors: Wu
authors: Zhen
authors: Z
authors: Yan
mesh_terms: D017209:Apoptosis; D001980:Bronchi / Q000166:cytology; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D003033:Coal Tar / Q000009:adverse effects*; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D012906:Smoke / Q000009:adverse effects
chemical_list: D053583:Interleukin-1beta; D012906:Smoke; D003033:Coal Tar; D007770:L-Lactate Dehydrogenase; D020170:Caspase 1