title: Synthesis, crystal structures, DNA binding and cytotoxicity of two novel platinum(II) complexes containing 2-(hydroxymethyl)pyridine and pyridine-2-carboxylate ligands.
abstract: Two new platinum(II) complexes, trans-[Pt(2-mpy)(2)]·4H(2)O (1) and [PtCl(2-pyc)(2-hmpy)]·H(2)O (2), where 2-hmpy=2-(hydroxymethyl)pyridine, 2-mpy=deprotonated 2-hmpy and 2-pyc=pyridine-2-carboxylate, have been synthesized and characterized by elemental analysis, IR, NMR, and X-ray crystallography. The DNA binding affinities of these complexes for Fish Sperm DNA (FS-DNA) were investigated using fluorescence, viscosity, thermal denaturation and gel electrophoresis measurements. Fluorescence analysis indicates that complex 1 binds to DNA by a single intercalative mechanism, while complex 2 exhibits two types of interactions such as intercalation and covalent binding. Gel electrophoresis assay demonstrates ability of the complexes to cleavage the supercoiled pBR322 plasmid DNA. The in vitro cytotoxicities of both complexes were preliminarily evaluated and the cytotoxicity of complex 1 against the human lung cancer cells (H1299) is similar to oxaliplatin, but higher than transplatin and carboplatin.
authors: Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
authors: Ceyda
authors: C
authors: Icsel
authors: Veysel T
authors: VT
authors: Yilmaz
authors: Aysegul
authors: A
authors: Golcu
authors: Engin
authors: E
authors: Ulukaya
authors: Orhan
authors: O
authors: Buyukgungor
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005399:Fishes; D006801:Humans; D008024:Ligands; D008297:Male; D008958:Models, Molecular; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010848:Picolinic Acids / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry* / Q000187:drug effects; D011725:Pyridines / Q000737:chemistry*; D051381:Rats; D013094:Spermatozoa / Q000737:chemistry* / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D008024:Ligands; D009944:Organoplatinum Compounds; D010848:Picolinic Acids; D011725:Pyridines; D004247:DNA; C030614:picolinic acid