title: Analysis of dentin/enamel remineralization by a CPP-ACP paste: AFM and SEM study.
abstract: The aim of the present in vitro study was the evaluation of a CPP-ACP paste on preventing dentin/enamel erosion produced by a soft drink; Atomic Force Microscopy and Scanning Electron Microscopy were used. Eighty extracted human incisors free of caries were selected and divided into four groups (each divided in two subgroups); group 1a: intact dentin; group 1b: dentin + soft drink; group 2a: intact dentin + CCP-ACP paste; group 2b: dentin + soft drink + CCP-ACP paste; group 3a: intact enamel; group 3b: enamel + soft drink; group 4a: intact enamel + CCP-ACP paste; group 4b: enamel + soft drink + CCP-ACP paste. The CPP-ACP paste was applied for 3 min at 0, 8, 24, and 36 h. The surface of each dentin/enamel specimen was imaged by AFM (Rrms values were registered) and SEM. A statistical significant difference was recorded between groups 1b (dentin + soft drink) and 2b (dentin + soft drink + CCP-ACP paste) and between groups 3b (enamel + soft drink) and 4b (enamel + soft drink + CCP-ACP paste), suggesting that treatment of the specimens with the CPP-ACP paste had a protective effect on enamel demineralization, which was more evident for enamel specimens. Under the limitations of the present in vitro study, it can be concluded that the application of a CPP-ACP paste is effective on preventing dentin/enamel erosion produced by a soft drink.
authors: Department of Operative Dentistry, Policlinico "San Matteo", Pavia, Italy.
authors: Claudio
authors: C
authors: Poggio
authors: Marco
authors: M
authors: Lombardini
authors: Paolo
authors: P
authors: Vigorelli
authors: Matteo
authors: M
authors: Ceci
mesh_terms: D001628:Beverages; D002364:Caseins / Q000627:therapeutic use*; D003743:Dental Enamel / Q000187:drug effects* / Q000648:ultrastructure*; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure*; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D013499:Surface Properties / Q000187:drug effects*
chemical_list: D002364:Caseins; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex
keywords: AFM; CPP-ACP; SEM; dentin; enamel; surface erosion