title: Soil microbial diversity, site conditions, shelter forest land, saline water drip-irrigation, drift desert.
abstract: Soil microbes in forest land are crucial to soil development in extreme areas. In this study, methods of conventional culture, PLFA and PCR-DGGE were utilized to analyze soil microbial quantity, fatty acids and microbial DNA segments of soils subjected to different site conditions in the Tarim Desert Highway forest land. The main results were as follows: the soil microbial amount, diversity indexes of fatty acid and DNA segment differed significantly among sites with different conditions (F < F0.05 ). Specifically, the values were higher in the middle and base of dunes than the top part of dunes and hardened flat sand, but all values for dunes were higher than for drift sand. Bacteria was dominant in the soil microbial community (>84%), followed by actinomycetes and then fungi (<0.05%). Vertical differences in the soil microbial diversity were insignificant at 0-35 cm. Correlation analysis indicated that the forest trees grew better as the soil microbial diversity index increased. Therefore, construction of the Tarim Desert Highway shelter-forest promoted soil biological development; however, for enhancing sand control efficiency and promoting sand development, we should consider the effects of site condition in the construction and regeneration of shelter-forest ecological projects.
authors: Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
authors: Zhengzhong
authors: Z
authors: Jin
authors: Jiaqiang
authors: J
authors: Lei
authors: Shengyu
authors: S
authors: Li
authors: Xinwen
authors: X
authors: Xu
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003889:Desert Climate; D005227:Fatty Acids / Q000737:chemistry; D065928:Forests; D005658:Fungi / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D014644:Genetic Variation; D010802:Phylogeny; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014197:Trees
chemical_list: D005227:Fatty Acids; D012987:Soil
keywords: DNA segment; Fatty acid; Microbial community; Site conditions; The Tarim Desert Highway shelter-forest