title: Fluorescence Cross-correlation Spectroscopy (FCCS) to Observe Dimerization of Transcription Factors in Living Cells.
abstract: Fluorescence cross-correlation spectroscopy (FCCS) is an established spectroscopic method to observe the interaction between the different fluorescent molecules. Using FCCS, researchers can assess the interaction of target molecules in the aqueous condition, and can apply the technique in cultured cells. Here, we describe the method of FCCS to demonstrate direct observation of dimerization between transcription factors in a living cell.
authors: Laboratory of Functional Biology, Tokushima Bunri University, Sanuki, Kagawa, Japan.
authors: Hisayo
authors: H
authors: Sadamoto
authors: Hideki
authors: H
authors: Muto
mesh_terms: D000465:Algorithms; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis; D018613:Microscopy, Confocal / Q000379:methods; D011485:Protein Binding; D055503:Protein Multimerization; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis; D059010:Single-Cell Analysis / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D000097573:Red Fluorescent Protein
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D000097573:Red Fluorescent Protein; C115163:CREB1 protein, human; C485184:enhanced green fluorescent protein