title: Topical vaccination with functionalized particles targeting dendritic cells.
abstract: Needle-free vaccination, for reasons of safety, economy, and convenience, is a central goal in vaccine development, but it also needs to meet the immunological requirements for efficient induction of prophylactic and therapeutic immune responses. Combining the principles of noninvasive delivery to dendritic cells (DCs) through skin and the immunological principles of cell-mediated immunity, we developed microparticle-based topical vaccines. We show here that the microparticles are efficient carriers for coordinated delivery of the essential vaccine constituents to DCs for cross-presentation of the antigens and stimulation of T-cell responses. When applied to the skin, the microparticles penetrate into hair follicles and target the resident DCs, the immunologically most potent cells and site for induction of efficient immune responses. The microparticle vaccine principle can be applied to different antigen formats such as peptides and proteins, or nucleic acids coding for the antigens.
authors: Department of Dermatology, Venerology and Allergology, Charité-Universitätsmedizin Berlin, Humboldt-Universität zu Berlin and Freie Universität Berlin, Berlin, Germany.
authors: Renato B
authors: RB
authors: Baleeiro
authors: Karl-Heinz
authors: KH
authors: Wiesmüller
authors: Yoran
authors: Y
authors: Reiter
authors: Barbara
authors: B
authors: Baude
authors: Lars
authors: L
authors: Dähne
authors: Alexa
authors: A
authors: Patzelt
authors: Jürgen
authors: J
authors: Lademann
authors: José A
authors: JA
authors: Barbuto
authors: Peter
authors: P
authors: Walden
mesh_terms: D000287:Administration, Topical; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000941:Antigens / Q000008:administration & dosage / Q000378:metabolism; D002470:Cell Survival; D055252:Cell-Derived Microparticles / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000378:metabolism; D011992:Endosomes / Q000276:immunology / Q000378:metabolism; D005260:Female; D018859:Hair Follicle / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D008297:Male; D010455:Peptides / Q000008:administration & dosage / Q000378:metabolism; D012822:Silicon Dioxide / Q000378:metabolism / Q000494:pharmacology; D012867:Skin / Q000276:immunology* / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014611:Vaccination / Q000379:methods*
chemical_list: D000941:Antigens; D010455:Peptides; D012822:Silicon Dioxide