title: Application of microarrays for deciphering the structure and function of the human glycome.
abstract: Glycan structures were defined historically using multiple methods to determine composition, sequence, linkage, and anomericity of component monosaccharides. Such approaches have been replaced by more sensitive MS methods to profile or predict glycan structures, but these methods are limited in their ability to completely define glycan structures. Glycan-binding proteins, including lectins and antibodies, have been found to have exquisite binding specificities that can provide information about glycan structures. Here, we show glycan-binding proteins can be used along with MS to help define glycan linkages and other determinants in unknown glycans printed as shotgun glycan microarrays.
authors: Department of Biochemistry and Glycomics Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA. dfsmith@emory.edu
authors: David F
authors: DF
authors: Smith
authors: Richard D
authors: RD
authors: Cummings
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D054794:Glycomics; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D008895:Milk, Human / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
chemical_list: D011134:Polysaccharides