title: Determination of ethyl carbamate in cachaça produced from copper stills by HPLC.
abstract: Ethyl carbamate (EC) is a common substance in fermented foods and drinks, and its quantification is important because of its carcinogenic nature and its usually presence in alcoholic beverages. The present work involved the development and validation of an analytical method for the evaluation of EC in cachaça by HPLC-FLD after previous derivatization with xanthydrol. The method presented a mean recovery of 94.88%, an intra-day precision of 4.19% (30.0 μgL(-1)) and 3.32% (75.0 μgL(-1)), a coefficient of determination (r(2)) equal to 0.9985, and limits of detection and quantification equal to 6.39 and 21.32 μgL(-1), respectively. The results show that the analytical method is accurate, reproducible and linear over the concentration range from 5.0 to 160 μg of EC per litre. The method was applied to the analysis of EC in cachaça, the analyses being rapid and efficient.
authors: Departamento de Ciência dos Alimentos, Universidade Federal de Lavras, Campus Universitário CP 3037, CEP 37200-000 Lavras, MG, Brazil.
authors: Ana Maria
authors: AM
authors: de Resende Machado
authors: M das Graças
authors: Md
authors: Cardoso
authors: Adelir Aparecida
authors: AA
authors: Saczk
authors: Jeancarlo Pereira
authors: JP
authors: dos Anjos
authors: Lidiany Mendonça
authors: LM
authors: Zacaroni
authors: Haroldo Silveira
authors: HS
authors: Dórea
authors: David Lee
authors: DL
authors: Nelson
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003300:Copper / Q000737:chemistry* / Q000633:toxicity; D057168:Distillation / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis; D014520:Urethane / Q000032:analysis*
chemical_list: D002273:Carcinogens; D014520:Urethane; D003300:Copper