title: Fermentation of levoglucosan with oleaginous yeasts for lipid production.
abstract: This paper reports the production of lipids from non-hydrolyzed levoglucosan (LG) by oleaginous yeasts Rhodosporidium toruloides and Rhodotorula glutinis. Enzyme activity tests of LG kinases from both yeasts indicated that the phosphorylation pathway of LG to glucose-6-phosphate existed. The highest enzyme activity obtained for R. glutinis was 0.22 U/mg of protein. The highest cell mass and lipid production by R. glutinis were 6.8 and 2.7 g/L, respectively from pure LG, and 3.3 and 0.78 g/L from a pyrolytic LG aqueous phase detoxified by ethyl acetate extraction, rotary evaporation and activated carbon. This corresponded to a lipid yield of 13.5 wt.% for pure LG and only 3.9 wt.% for LG in pyrolysis oil.
authors: Biological Systems Engineering Department, Washington State University, Pullman, WA 99164-6120, USA.
authors: Jieni
authors: J
authors: Lian
authors: Manuel
authors: M
authors: Garcia-Perez
authors: Shulin
authors: S
authors: Chen
mesh_terms: D001487:Basidiomycota / Q000201:enzymology / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism; D006868:Hydrolysis; D008055:Lipids / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism; D028224:Pseudotsuga / Q000378:metabolism; D012248:Rhodotorula / Q000201:enzymology / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D008055:Lipids; C014989:1,6-anhydro-beta-glucopyranose; D010770:Phosphotransferases; D005947:Glucose