title: Visualizing brain inflammation with a shingled-leg radio-frequency head probe for 19F/1H MRI.
abstract: Magnetic resonance imaging (MRI) provides the opportunity of tracking cells in vivo. Major challenges in dissecting cells from the recipient tissue and signal sensitivity constraints albeit exist. In this study, we aimed to tackle these limitations in order to study inflammation in autoimmune encephalomyelitis. We constructed a very small dual-tunable radio frequency (RF) birdcage probe tailored for (19)F (fluorine) and (1)H (proton) MR mouse neuroimaging. The novel design eliminated the need for extra electrical components on the probe structure and afforded a uniform -field as well as good SNR. We employed fluorescently-tagged (19)F nanoparticles and could study the dynamics of inflammatory cells between CNS and lymphatic system during development of encephalomyelitis, even within regions of the brain that are otherwise not easily visualized by conventional probes. (19)F/(1)H MR Neuroimaging will allow us to study the nature of immune cell infiltration during brain inflammation over an extensive period of time.
authors: Berlin Ultrahigh Field Facility (BUFF), Max Delbrück Center for Molecular Medicine, Germany. helmar.waiczies@mdc-berlin.de
authors: Helmar
authors: H
authors: Waiczies
authors: Stefano
authors: S
authors: Lepore
authors: Susanne
authors: S
authors: Drechsler
authors: Fatimunnisa
authors: F
authors: Qadri
authors: Bettina
authors: B
authors: Purfürst
authors: Karl
authors: K
authors: Sydow
authors: Margitta
authors: M
authors: Dathe
authors: André
authors: A
authors: Kühne
authors: Tomasz
authors: T
authors: Lindel
authors: Werner
authors: W
authors: Hoffmann
authors: Andreas
authors: A
authors: Pohlmann
authors: Thoralf
authors: T
authors: Niendorf
authors: Sonia
authors: S
authors: Waiczies
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000503:physiopathology*; D004867:Equipment Design; D005461:Fluorine; D008279:Magnetic Resonance Imaging / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D053758:Nanoparticles; D059906:Neuroimaging / Q000295:instrumentation* / Q000379:methods; D011522:Protons
chemical_list: D011522:Protons; D005461:Fluorine