title: Montmorillonite clay based polyurethane nanocomposite as substrate for retinal pigment epithelial cell growth.
abstract: The subretinal transplantation of retinal pigment epithelial cells (RPE cells) grown on polymeric supports may have interest in retinal diseases affecting RPE cells. In this study, montmorillonite based polyurethane nanocomposite (PU-NC) was investigated as substrate for human RPE cell growth (ARPE-19 cells). The ARPE-19 cells were seeded on the PU-NC, and cell viability, proliferation and differentiation were investigated. The results indicated that ARPE-19 cells attached, proliferated onto the PU-NC, and expressed occludin. The in vivo ocular biocompatibility of the PU-NC was assessed by using the HET-CAM; and through its implantation under the retina. The direct application of the nanocomposite onto the CAM did not compromise the vascular tissue in the CAM surface, suggesting no ocular irritancy of the PU-NC film. The nanocomposite did not elicit any inflammatory response when implanted into the eye of rats. The PU-NC may have potential application as a substrate for RPE cell transplantation.
authors: School of Pharmacy, Federal University of São João Del Rei, Av. Sebastião Gonçalves Coelho 400, Chanadour, Divinópolis, Minas Gerais 35500-296, Brazil. giselersilva@ufsj.edu.br
authors: Gisele Rodrigues
authors: GR
authors: Da Silva
authors: Armando
authors: A
authors: Da Silva-Cunha
authors: Lorena Carla
authors: LC
authors: Vieira
authors: Lívia Mara
authors: LM
authors: Silva
authors: Eliane
authors: E
authors: Ayres
authors: Rodrigo Lambert
authors: RL
authors: Oréfice
authors: Silvia Ligório
authors: SL
authors: Fialho
authors: Juliana Barbosa
authors: JB
authors: Saliba
authors: Francine
authors: F
authors: Behar-Cohen
mesh_terms: D000538:Aluminum Silicates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001546:Bentonite / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D000077215:Clay; D005260:Female; D006801:Humans; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry; D011140:Polyurethanes / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D011914:Rats, Inbred BN; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000538:Aluminum Silicates; D011140:Polyurethanes; D001546:Bentonite; D000077215:Clay