title: 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH).
abstract: We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from  chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with  low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of  Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.
authors: Department of Genetics, Northeastern Biomedical Research Center, The Mexican Social Security Institute (IMSS), 64720 Monterrey, Mexico. elvacortes@cibinmty.net.
authors: Elva I
authors: EI
authors: Cortés-Gutiérrez
authors: Brenda L
authors: BL
authors: Ortíz-Hernández
authors: Martha I
authors: MI
authors: Dávila-Rodríguez
authors: Ricardo M
authors: RM
authors: Cerda-Flores
authors: José Luis
authors: JL
authors: Fernández
authors: Carmen
authors: C
authors: López-Fernández
authors: Jaime
authors: J
authors: Gosálvez