title: Wessim: a whole-exome sequencing simulator based on in silico exome capture.
abstract: CONCLUSIONS
  We propose a targeted re-sequencing simulator Wessim that generates synthetic exome sequencing reads from a given sample genome. Wessim emulates conventional exome capture technologies, including Agilent's SureSelect and NimbleGen's SeqCap, to generate DNA fragments from genomic target regions. The target regions can be either specified by genomic coordinates or inferred from in silico probe hybridization. Coupled with existing next-generation sequencing simulators, Wessim generates a realistic artificial exome sequencing data, which is essential for developing and evaluating exome-targeted variant callers.
  
  
  BACKGROUND
  Source code and the packaged version of Wessim with manuals are available at http://sak042.github.com/Wessim/.
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors: Department of Computer Science and Engineering and Department of Electrical and Computer Engineering, University of California at San Diego, La Jolla, CA 92093, USA. sak042@cs.ucsd.edu
authors: Sangwoo
authors: S
authors: Kim
authors: Kyowon
authors: K
authors: Jeong
authors: Vineet
authors: V
authors: Bafna
mesh_terms: D003198:Computer Simulation; D059472:Exome; D015894:Genome, Human; D023281:Genomics / Q000379:methods; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software