title: Creation of a completely helper cell-dependent recombinant morbillivirus.
abstract: We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P protein-deleted virus (P(-)) grew very inefficiently unless both of the viral accessory proteins (V and C) were also expressed. Growth of the virus was restricted to the P-expressing cell line. The P(-) virus grew more slowly than the parental virus and expressed much less viral protein in infected cells. The technique could be used to create virus-like particles for use as a vaccine or as antigen in immunological or serological assays.
authors: The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UK.
authors: Jana
authors: J
authors: Baron
authors: The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UK.
authors: Michael
authors: M
authors: Baron
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D017353:Gene Deletion; D005818:Genetic Engineering; D006801:Humans; D018103:Morbillivirus / Q000235:genetics* / Q000502:physiology; D018185:Morbillivirus Infections / Q000821:virology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism / Q000821:virology*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D010750:Phosphoproteins; D014764:Viral Proteins