title: Enhanced maintenance of rat islets of Langerhans on laminin-coated  electrospun nanofibrillar matrix in vitro.
abstract: Understanding the extracellular matrix (ECM) effect on pancreatic β cells is critical to optimise the derivation of functional β cells for transplantation and understand mechanisms that control islet neogenesis and glucose homeostasis. We assessed the effect of natural ECMs [collagen I, collagen IV, laminin and fibronectin (FN)] on rat islets of Langerhans' morphology, adhesion, viability, functionality and islet specific genes expression after 7 days in vitro culture. However, we could not detect a significant difference on the other parameters in these ECMs and islets interaction. To examine islets interactions, we used a synthetic three dimensional surface composed of electrospun polyamide nanofibres. Laminin-coated nanofibrillar surfaces, but not laminin or nanosurface alone, induced comparable expression of the Ins1 and Ins2 genes in adult β cells. Using a glucose challenge test, a marked response of insulin secretion by islets occurred that were cultured on laminin-coated nanofibrillar surfaces.We contend that the reestablishment of cellular interactions by the combination of nanomaterials and natural ECMs can be useful in maintaining in vitro islet functions.
authors: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR,
authors: Mozhdeh
authors: M
authors: Sojoodi
authors: Ali
authors: A
authors: Farrokhi
authors: Azadeh
authors: A
authors: Moradmand
authors: Hossein
authors: H
authors: Baharvand
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D055664:Electrochemical Techniques; D015870:Gene Expression; D007515:Islets of Langerhans / Q000166:cytology / Q000502:physiology*; D007783:Lactones; D007797:Laminin / Q000737:chemistry*; D008297:Male; D057139:Nanofibers / Q000737:chemistry*; D017208:Rats, Wistar; D046509:Tissue Culture Techniques
chemical_list: D020099:Coated Materials, Biocompatible; C118715:LL Z1640-2; D007783:Lactones; D007797:Laminin