title: Complete Genome Sequence of Paenibacillus strain Y4.12MC10, a Novel Paenibacillus lautus strain Isolated from Obsidian Hot Spring in Yellowstone National Park.
abstract: Paenibacillus sp.Y412MC10 was one of a number of organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The isolate was initially classified as a Geobacillus sp. Y412MC10 based on its isolation conditions and similarity to other organisms isolated from hot springs at Yellowstone National Park. Comparison of 16 S rRNA sequences within the Bacillales indicated that Geobacillus sp.Y412MC10 clustered with Paenibacillus species, and the organism was most closely related to Paenibacillus lautus. Lucigen Corp. prepared genomic DNA and the genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute. The genome sequence was deposited at the NCBI in October 2009 (NC_013406). The genome of Paenibacillus sp. Y412MC10 consists of one circular chromosome of 7,121,665 bp with an average G+C content of 51.2%. Comparison to other Paenibacillus species shows the organism lacks nitrogen fixation, antibiotic production and social interaction genes reported in other paenibacilli. The Y412MC10 genome shows a high level of synteny and homology to the draft sequence of Paenibacillus sp. HGF5, an organism from the Human Microbiome Project (HMP) Reference Genomes. This, combined with genomic CAZyme analysis, suggests an intestinal, rather than environmental origin for Y412MC10.
authors: Lucigen Corporation, Middleton, Wisconsin ; DOE Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Wisconsin.
authors: David A
authors: DA
authors: Mead
authors: Susan
authors: S
authors: Lucas
authors: Alex
authors: A
authors: Copeland
authors: Alla
authors: A
authors: Lapidus
authors: Jan-Feng
authors: JF
authors: Cheng
authors: David C
authors: DC
authors: Bruce
authors: Lynne A
authors: LA
authors: Goodwin
authors: Sam
authors: S
authors: Pitluck
authors: Olga
authors: O
authors: Chertkov
authors: Xiaojing
authors: X
authors: Zhang
authors: John C
authors: JC
authors: Detter
authors: Cliff S
authors: CS
authors: Han
authors: Roxanne
authors: R
authors: Tapia
authors: Miriam
authors: M
authors: Land
authors: Loren J
authors: LJ
authors: Hauser
authors: Yun-Juan
authors: YJ
authors: Chang
authors: Nikos C
authors: NC
authors: Kyrpides
authors: Natalia N
authors: NN
authors: Ivanova
authors: Galina
authors: G
authors: Ovchinnikova
authors: Tanja
authors: T
authors: Woyke
authors: Catherine
authors: C
authors: Brumm
authors: Rebecca
authors: R
authors: Hochstein
authors: Thomas
authors: T
authors: Schoenfeld
authors: Phillip
authors: P
authors: Brumm
keywords: Geobacillus sp. Y412MC10; Obsidian Hot Spring; Paenibacillus sp. Y412MC10