title: Splenic marginal zone lymphoma: comprehensive analysis of gene expression and miRNA profiling.
abstract: Splenic marginal zone lymphoma is a small B-cell neoplasm whose molecular pathogenesis is still essentially unknown and whose differentiation from other small B-cell lymphomas is hampered by the lack of specific markers. We have analyzed the gene expression and miRNA profiles of 31 splenic marginal zone lymphoma cases. For comparison, 7 spleens with reactive lymphoid hyperplasia, 10 spleens infiltrated by chronic lymphocytic leukemia, 12 spleens with follicular lymphoma, 6 spleens infiltrated by mantle cell lymphoma and 15 lymph nodes infiltrated by nodal marginal zone lymphoma were included. The results were validated by qRT-PCR in an independent series including 77 paraffin-embedded splenic marginal zone lymphomas. The splenic marginal zone lymphoma miRNA signature had deregulated expression of 51 miRNAs. The most highly overexpressed miRNAs were miR-155, miR-21, miR-34a, miR-193b and miR-100, while the most repressed miRNAs were miR-377, miR-27b, miR-145, miR-376a and miR-424. MiRNAs located in 14q32-31 were underexpressed in splenic marginal zone lymphoma compared with reactive lymphoid tissues and other B-cell lymphomas. Finally, the gene expression data were integrated with the miRNA profile to identify functional relationships between genes and deregulated miRNAs. Our study reveals miRNAs that are deregulated in splenic marginal zone lymphoma and identifies new candidate diagnostic molecules for splenic marginal zone lymphoma.
authors: Genetics Department, Virgen de la Salud Hospital, Toledo, CP 45004 Spain. aarribas@sescam.jccm.es
authors: Alberto J
authors: AJ
authors: Arribas
authors: Cristina
authors: C
authors: Gómez-Abad
authors: Margarita
authors: M
authors: Sánchez-Beato
authors: Nerea
authors: N
authors: Martinez
authors: Lorena
authors: L
authors: Dilisio
authors: Felipe
authors: F
authors: Casado
authors: Miguel A
authors: MA
authors: Cruz
authors: Patrocinio
authors: P
authors: Algara
authors: Miguel A
authors: MA
authors: Piris
authors: Manuela
authors: M
authors: Mollejo
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D013160:Splenic Neoplasms / Q000235:genetics*; D059467:Transcriptome
chemical_list: D035683:MicroRNAs