title: Catalase gene C-262T polymorphism: importance in ulcerative colitis.
abstract: OBJECTIVE
  Reactive oxygen species (ROS) are by-products of the cellular metabolism and have important roles in the normal physiology of the cell. However, when ROS production exceeds the antioxidant capacity, a state known as oxidative stress, damage to cellular macromolecules emerges. A crucial role in counteracting ROS is played by the enzyme catalase. A common polymorphism in the catalase (CAT) promoter region (C-262T) alters the expression as well as blood catalase levels, and leads to a number of human diseases. Ulcerative colitis (UC) is an inflammatory condition of the large bowel that is known to be influenced by oxidative stress. In this study, we aimed to evaluate the association of CAT C-262T polymorphism on the risk of UC.
  
  
  METHODS
  Samples were collected from 60 patients diagnosed with UC and 78 control subjects, and genotyped by allele-specific polymerase chain reaction.
  
  
  RESULTS
  We found that CAT C-262T genotype frequencies were significantly different between cases and controls (P = 0.002). Individuals carrying the -262C/T genotype had a greater risk for UC compared with C/C genotype (odds ratio, 4.88; 95% confidence interval, 1.73-13.75, P = 0.002).
  
  
  CONCLUSIONS
  This study indicates that CAT C-262T polymorphism may be associated with UC, and that the -262C/T genotype may be a risk factor for the disease. Further studies are needed to confirm the results.
authors: Department of Biology, Pardis International, University of Guilan, Rasht, Iran.
authors: Siamak
authors: S
authors: Khodayari
authors: Zivar
authors: Z
authors: Salehi
authors: Saba
authors: S
authors: Fakhrieh Asl
authors: Keyvan
authors: K
authors: Aminian
authors: Nadia
authors: N
authors: Mirzaei Gisomi
authors: Saeideh
authors: S
authors: Torabi Dalivandan
mesh_terms: D000328:Adult; D002374:Catalase / Q000097:blood / Q000235:genetics* / Q000502:physiology*; D003093:Colitis, Ulcerative / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012307:Risk Factors; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species; D002374:Catalase