title: Nonlabens arenilitoris sp. nov., a member of the family Flavobacteriaceae isolated from seashore sand.
abstract: A Gram-staining-negative, non-spore-forming, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated M-M3(T), was isolated from marine sand on the southern coast of South Korea and subjected to a polyphasic study. Strain M-M3(T) was observed to grow optimally at 25-30 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M3(T) fell within the clade comprising Nonlabens species, joining the type strain of Nonlabens ulvanivorans, with which it exhibited the highest 16S rRNA gene sequence similarity value of 99.4 %. Sequence similarity to the type strains of the other Nonlabens species was 93.0-95.2 % and <92.2 % to those of other species used in the phylogenetic analysis. Strain M-M3(T) was found to contain MK-6 as the predominant menaquinone and iso-C15:0 and iso-C17:0 3-OH as the major fatty acids. The major polar lipids were determined to be phosphatidylethanolamine, five unidentified lipids and one unidentified aminolipid. The DNA G+C content of strain M-M3(T) was determined to be 38.2 mol% and its DNA-DNA relatedness values with N. ulvanivorans DSM 22727(T) was 42 ± 6.2 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain M-M3(T) is separate from other Nonlabens species. On the basis of the data presented, strain M-M3(T) is considered to represent a novel species of the genus Nonlabens, for which the name Nonlabens arenilitoris sp. nov. is proposed. The type strain is M-M3(T) (=KCTC 32109(T) = CCUG 62919(T)).
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sooyeon
authors: S
authors: Park
authors: Chul-Hyung
authors: CH
authors: Kang
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D000332:Aerobiosis; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; D012822:Silicon Dioxide