title: [Tobacco--a producer of recombinant interleukins].
abstract: Interleukins are cytokines of highly pleiotropic activity and they have high potential for application in the treatment of cancer and autoimmune diseases. Trials of recombinant interleukin production in plants relate almost exclusively to tobacco, where through the transformation of the nuclear genome (agroinfection) monomeric (IL-2, IL-4, IL-13, IL-18), homodimeric (IL-10) and single-chain heterodimeric (IL-12) interleukins have been obtained. The expression of IL-10 as a homodimer in the chloroplast genome could not be reached. Expression of the given interleukin was obtained in the leaves, cell culture and culture of hairy roots of tobacco. Interleukins obtained in tobacco showed similar in vitro biological activity as commercial ILs produced mostly in E. coli. Glycosylated IL-13 obtained in tobacco was much more resistant to proteolytic digestion than commercial non-glycosylated IL-13; therefore in the case of sufficiently large production it could be suitable for oral administration in the treatment of type I diabetes.
authors: Katedra i Zakład Botaniki Farmaceutycznej i Biotechnologii Roślin, Uniwersytet Medyczny im. Karola Marcinkowskiego, 61-861 Poznań, ul. Sw. Marii Magdaleny 14. jbudzian@ump.edu.pl
authors: Jaromir
authors: J
authors: Budzianowski
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis; D018793:Interleukin-13 / Q000096:biosynthesis / Q000627:therapeutic use; D020382:Interleukin-18 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007378:Interleukins / Q000032:analysis* / Q000096:biosynthesis*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis* / Q000096:biosynthesis*; D014026:Nicotiana / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D018793:Interleukin-13; D020382:Interleukin-18; D007376:Interleukin-2; D007378:Interleukins; D011994:Recombinant Proteins; D018664:Interleukin-12; D015847:Interleukin-4