title: Analysis of F2-isoprostanes in plasma of pregnant women by HPLC-MS/MS using a column packed with core-shell particles.
abstract: Plasma F2-isoprostanes (F2-isoPs) are reliable biomarkers of oxidative stress. Several possible F2-isoPs are generated by the oxidation of arachidonic acid esterified in phospholipids. The separation of these isomers represents a technical challenge for rapid and selective determination. We have developed a HPLC-MS/MS method for the simultaneous determination of seven plasma F2-isoPs, namely 8-iso-15(R)-prostaglandin F2α (PGF2α), 8-iso-PGF2α, 15(R)-PGF2α, iPF2α-IV, iPF2α-VI, 5-iPF2α-VI, and (±)5-8,12-iso-iPF2α-VI. We have validated this method in plasma of pregnant women, a mild physiological oxidative stress known to increase F2-isoPs. Thus, plasma samples of women collected at the third trimester of pregnancy (n = 20) were subjected to alkaline hydrolysis followed by liquid-liquid extraction in order to extract total F2-isoPs. The F2-isoPs were separated within 16.5 min using a column packed with core-shell particles. The class VI isomers were the most abundant, accounting for 65% of the total level of all quantified F2-isoPs in plasma of pregnant women (P < 0.05). The 15(R)-PGF2α was the most abundant of the class III isomers quantified. This method allowed fast and selective separation of seven isomers from three different classes of F2-isoP regioisomers.
authors: Axe reproduction, santé de la mère et de l'enfant, et Centre de Recherche en Biologie de la Reproduction (CRBR), and Centre de recherche du CHU de Québec, Québec, Canada.
authors: Jessica
authors: J
authors: Larose
authors: Pierre
authors: P
authors: Julien
authors: Jean-François
authors: JF
authors: Bilodeau
mesh_terms: D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028441:F2-Isoprostanes / Q000097:blood / Q000145:classification / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007536:Isomerism; D018384:Oxidative Stress; D011247:Pregnancy; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes