title: Phagomagnetic separation and electrochemical magneto-genosensing of pathogenic bacteria.
abstract: This paper addresses the use of bacteriophages immobilized on magnetic particles for the biorecognition of the pathogenic bacteria, followed by electrochemical magneto-genosensing of the bacteria. The P22 bacteriophage specific to Salmonella (serotypes A, B, and D1) is used as a model. The bacteria are captured and preconcentrated by the bacteriophage-modified magnetic particles through the host interaction with high specificity and efficiency. DNA amplification of the captured bacteria is then performed by double-tagging polymerase chain reaction (PCR). Further detection of the double-tagged amplicon is achieved by electrochemical magneto-genosensing. The strategy is able to detect in 4 h as low as 3 CFU mL(-1) of Salmonella in Luria-Bertani (LB) media. This approach is compared with conventional culture methods and PCR-based assay, as well as with immunological screening assays for bacteria detection, highlighting the outstanding stability and cost-efficient and animal-free production of bacteriophages as biorecognition element in biosensing devices.
authors: Departament de Química, and Unitat de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors: Susana
authors: S
authors: Liébana
authors: Denis A
authors: DA
authors: Spricigo
authors: María Pilar
authors: MP
authors: Cortés
authors: Jordi
authors: J
authors: Barbé
authors: Montserrat
authors: M
authors: Llagostera
authors: Salvador
authors: S
authors: Alegret
authors: María Isabel
authors: MI
authors: Pividori
mesh_terms: D017100:Bacteriophage P22 / Q000737:chemistry* / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D060306:Magnetometry / Q000379:methods; D012483:Salmonella Phages / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D058185:Magnetite Nanoparticles