title: Novel electrochemical biosensor based on functional composite nanofibers for sensitive detection of p53 tumor suppressor gene.
abstract: A novel electrochemical biosensor based on functional composite nanofibers for sensitive hybridization detection of p53 tumor suppressor using methylene blue (MB) as an electrochemical indicator is developed. The carboxylated multi-walled carbon nanotubes (MWNTs) doped nylon 6 (PA6) composite nanofibers (MWNTs-PA6) was prepared using electrospinning, which served as the nanosized backbone for pyrrole (Py) electropolymerization. The functional composite nanofibers (MWNTs-PA6-PPy) used as supporting scaffolds for ssDNA immobilization can dramatically increase the amount of DNA attachment and the hybridization sensitivity. The biosensor displayed good sensitivity and specificity. The target wild type p53 sequence (wtp53) can be detected as low as 50 fM and the discrimination is up to 57.5% between the wtp53 and the mutant type p53 sequence (mtp53). It holds promise for the early diagnosis of cancer development and monitoring of patient therapy.
authors: Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. wxy@seu.edu.cn
authors: Xiaoying
authors: X
authors: Wang
authors: Xiaobing
authors: X
authors: Wang
authors: Xiaoning
authors: X
authors: Wang
authors: Fentian
authors: F
authors: Chen
authors: Kehui
authors: K
authors: Zhu
authors: Qian
authors: Q
authors: Xu
authors: Meng
authors: M
authors: Tang
mesh_terms: D015374:Biosensing Techniques; D002209:Caprolactam / Q000031:analogs & derivatives / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry; D009154:Mutation; D057139:Nanofibers / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded; D061925:Immobilized Nucleic Acids; D037742:Nanotubes, Carbon; D011108:Polymers; D016159:Tumor Suppressor Protein p53; C009916:nylon 6; D002209:Caprolactam; D008751:Methylene Blue