title: Biological approach to synthesize TiO2 nanoparticles using Aeromonas hydrophila and its antibacterial activity.
abstract: Nanosized materials have been an important subject in basic and applied sciences. A novel, low-cost, green and reproducible bacteria, Aeromonas hydrophila mediated biosynthesis of titanium dioxide nanoparticles (TiO2 NPs) was reported. The resulting nanoparticles were characterized by FTIR, XRD, AFM and FESEM with EDX. FTIR showed characteristic bands (1643 and 3430 cm(-1)) finds the role of carboxyl group OH stretching amine NH stretch in the formation of TiO2 NPs. The XRD spectrum confirmed that the synthesized TiO2 NPs were in the form of nanocrystals, as evidenced by the peaks at 2θ values of 27.47°, 31.77°, 36.11°, 41.25°, 54.39°, 56.64° and 69.54° were identified as 110, 100, 101, 111, 211, 220 and 301 reflections, respectively. The crystallite sizes were calculated using Scherrer's formula applied to the major intense peaks and found to be the size of 40.50 nm. The morphological characterization was analyzed by FESEM and the analysis showed the NPs smooth shaped, spherical and uneven. GC-MS analysis showed the main compounds found in A. hydrophila were uric acid (2.95%), glycyl-L-glutamic acid (6.90%), glycyl-L-proline (74.41%) and L-Leucyl-D-leucine (15.74%). The potential glycyl-L-proline could have played an important role as a capping agent. A possible mechanism for the biosynthesis of TiO2 NPs has been proposed. The antibacterial activity of the synthesized TiO2 NPs was assessed by well diffusion method toward A. hydrophila, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis and showed effective inhibitory activity against S. aureus (33 mm) and S. pyogenes (31 mm).
authors: Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Vellore 632 509, Tamil Nadu, India.
authors: Chidambaram
authors: C
authors: Jayaseelan
authors: Abdul Abdul
authors: AA
authors: Rahuman
authors: Selvaraj Mohana
authors: SM
authors: Roopan
authors: Arivarasan Vishnu
authors: AV
authors: Kirthi
authors: Jayachandran
authors: J
authors: Venkatesan
authors: Se-Kwon
authors: SK
authors: Kim
authors: Moorthy
authors: M
authors: Iyappan
authors: Chinnadurai
authors: C
authors: Siva
mesh_terms: D016980:Aeromonas hydrophila / Q000737:chemistry* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism; D055772:Green Chemistry Technology / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D004151:Dipeptides; C009495:titanium dioxide; C015248:glycylproline; D014025:Titanium