title: Blood group genotyping in multi-transfused patients.
abstract: BACKGROUND
  In chronically transfused patients, the classical hemagglutination assays may be inaccurate in defining the RBC phenotypes of the patients due to previous transfusions.
  
  
  METHODS
  DNA samples from 39 multi-transfused patients including thalassemia and sickle cell disease were used for red blood cell genotyping. The Rh-Type and KKD-Type (BAGene, BAG Healthcare) were used to determine the polymorphisms associated with antigen expression for RHD, RHCE and Kell, Kidd, Duffy blood group systems, respectively. Results were compared with previously determined phenotyping results for RhD, RhCcEe and Kell by hemagglutination method.
  
  
  RESULTS
  Nineteen out of the 37(51%) patients had discrepancies between genotyping and phenotyping results in a total of 25 alleles. In 12 patients, the discrepancies had the potential of alloimmunization.
  
  
  CONCLUSIONS
  Blood group genotyping has vital importance in transfusion management of chronically transfused patients especially if the patients were not phenotyped before starting the initial transfusions.
authors: Ankara University Medical School, Department of Hematology, Ankara, Turkey. sulemine@yahoo.com
authors: Sule Mine
authors: SM
authors: Bakanay
authors: Aysenur
authors: A
authors: Ozturk
authors: Talia
authors: T
authors: Ileri
authors: Elif
authors: E
authors: Ince
authors: Suzan
authors: S
authors: Yavasoglu
authors: Nejat
authors: N
authors: Akar
authors: Zumrut
authors: Z
authors: Uysal
authors: Onder
authors: O
authors: Arslan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001789:Blood Group Antigens / Q000097:blood / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001803:Blood Transfusion; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged
chemical_list: D001789:Blood Group Antigens