title: Expression and characterization of the nitrile reductase queF from E. coli.
abstract: The expression and characterization of a nitrile reductase from Escherichia coli K-12 (EcoNR), a newly discovered enzyme class, is described. This enzyme has a potential application for an alternative nitrile reduction pathway. The enzyme activity towards its natural substrate, preQ(0), was demonstrated and optimal working conditions were found to be at 37°C and at pH 7 with Tris buffer.
authors: Biokatalyse & Organische Chemie, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft, The Netherlands.
authors: Kathrin
authors: K
authors: Moeller
authors: Giang-Son
authors: GS
authors: Nguyen
authors: Frank
authors: F
authors: Hollmann
authors: Ulf
authors: U
authors: Hanefeld
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D020134:Catalytic Domain; D004795:Enzyme Stability; D048168:Escherichia coli K12 / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D009704:Nucleoside Q / Q000096:biosynthesis; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D011744:Pyrimidinones / Q000378:metabolism; D011758:Pyrroles / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: C096876:7-(aminomethyl)-7-deazaguanine; D029968:Escherichia coli Proteins; D011744:Pyrimidinones; D011758:Pyrroles; D011993:Recombinant Fusion Proteins; D009249:NADP; D009704:Nucleoside Q; D010088:Oxidoreductases; C582776:QueF protein, E coli