title: 5-Hydroxymethylfurfural induces ADH7 and ARI1 expression in tolerant industrial Saccharomyces cerevisiae strain P6H9 during bioethanol production.
abstract: The aims of this work were to obtain, by evolutionary engineering, an industrial strain of Saccharomyces cerevisiae tolerant to high concentrations of HMF and to determine the expression levels of genes previously described as responsible for this tolerance. Cells were grown under anaerobic and oxygen limited conditions, in the presence of glucose or sucrose as carbon sources. P6H9 strain presented high expression levels for genes ADH7 and ARI1 in presence of HMF. This tolerant strain also showed higher ethanol productivity, biomass formation and alcohol dehydrogenase activity comparing to sensitive strains. Results suggest that S. cerevisiae P6H9 strain presents potential to be used for second-generation ethanol production.
authors: Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Nicole Teixeira
authors: NT
authors: Sehnem
authors: Angela da Silva
authors: Ada S
authors: Machado
authors: Fernanda Cristina Bezerra
authors: FC
authors: Leite
authors: Will de Barros
authors: Wde B
authors: Pita
authors: Marcos Antonio
authors: MA
authors: de Morais
authors: Marco Antônio Záchia
authors: MA
authors: Ayub
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005818:Genetic Engineering; D007218:Industrial Microbiology; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D056804:Biofuels; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; C470097:ADH7 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; D000449:Aldehyde Reductase; C576707:Ari1 protein, S cerevisiae