title: A novel reusable PAni-PVA-Amylase film: activity and analysis.
abstract: Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25°C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe(2+) inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity.
authors: Department of Molecular Biology and Biotechnology, Tezpur (Central) University, Tezpur, P.O.-Napaam-784028, Sonitpur, Assam, India.
authors: Susmita
authors: S
authors: Singh
authors: Jyoti Prasad
authors: JP
authors: Saikia
authors: Alak Kumar
authors: AK
authors: Buragohain
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D017743:Equipment Reuse; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D000516:alpha-Amylases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; C416807:polyaniline; D011142:Polyvinyl Alcohol; D000516:alpha-Amylases; D005976:Glutaral