title: Effect of tocopherol on biochemical blood parameters in pleuritis-induced rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin.
abstract: The aim of this study was to evaluate the effect of tocopherol on pleuritis-induced rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were treated with a single TCDD dose of 5 μg/kg body weight (b.w.) and then for 3 weeks they were daily supplemented with tocopherol at a dose of 30 mg/kg b.w. The inflammation was initiated by intrapleural injection of a single dose of 1% carrageenin solution in a volume of 0.15 ml. Changes in biochemical blood parameters were measured three times at the 24th, 72nd and 120th hour of pleuritis and the blood was collected from 20 animals of each group of rats (group with the control inflammation; group treated with TCDD and with control inflammation; group treated with TCDD, supplemented with tocopherol and with the inflammation). The following biochemical parameters were measured: tumor necrosis factor (TNF), interleukin-1 (IL-1), IL-2, IL-4, IL-6, procollagen, telopeptide, fibrinogen, cholesterol, urea, creatinine, aspartate aminotransferase (AspAT) and alanine aminotransferase (AlAT). Daily supplementation of tocopherol caused significant changes in the level of TNF, IL-1, IL-4, IL-6, urea, creatinine, AspAT and AlAT. According to the results of these studies, we suggest that tocopherol supplementation in high doses could act as a protective treatment to improve liver metabolism.
authors: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Poland i.calkosinski@wp.pl.
authors: Ireneusz
authors: I
authors: Całkosiński
authors: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Poland.
authors: Joanna
authors: J
authors: Rosińczuk-Tonderys
authors: Department of Medical Biochemistry, Wroclaw Medical University, Poland.
authors: Agnieszka
authors: A
authors: Bronowicka-Szydełko
authors: Department of Medical Biochemistry, Wroclaw Medical University, Poland.
authors: Katarzyna
authors: K
authors: Dzierzba
authors: Department of Medical Biochemistry, Wroclaw Medical University, Poland Department of Medical Biochemistry, Wroclaw Medical University, Poland.
authors: Justyna
authors: J
authors: Bazan
authors: Department of Conservative Dentistry and Pedodontics, Wroclaw Medical University, Poland.
authors: Maciej
authors: M
authors: Dobrzyński
authors: Department of Diagnostics Laboratory, 4th Military Academic Hospital in Wroclaw, Poland.
authors: Jacek
authors: J
authors: Majda
authors: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Poland Polish Academy of Sciences, Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
authors: Andrzej
authors: A
authors: Gamian
mesh_terms: D000818:Animals; D015415:Biomarkers; D002351:Carrageenan / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D018836:Inflammation Mediators / Q000378:metabolism*; D010998:Pleurisy / Q000139:chemically induced; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D051381:Rats; D024505:Tocopherols / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D000072317:Polychlorinated Dibenzodioxins; D002351:Carrageenan; D024505:Tocopherols
keywords: Biochemical parameters; dioxin; inflammation; oxidative stress; tocopherol