title: Paeonol suppresses lipopolysaccharide-induced inflammatory cytokines in macrophage cells and protects mice from lethal endotoxin shock.
abstract: Paeonol (2'-hydroxy-4'-methoxyacetophenone) is the main phenolic compound of the radix of Paeonia suffruticosa which has been used as traditional Chinese medicine. In this study, we primarily investigated the anti-inflammatory effects and the underlying mechanisms of paeonol in RAW macrophage cells; and based on these effects, we assessed the protective effects of paeonol on lipopolysaccharide-induced endotoxemia in mice. The in vitro study showed that paeonol regulated the production of TNF-α, IL-1β, IL-6, and IL-10 via inactivation of IκBα, ERK1/2, JNK, and p38 MAPK. In mouse model of lipopolysaccharide-induced endotoxemia, pro- and anti-inflammatory cytokines are significantly regulated, and thus the survival rates of lipolysaccharide-challenged mice are improved by paeonol (150, 200, or 250 mg/kg). Therefore, paeonol has a beneficial activity against lipopolysaccharide-induced inflammation in RAW 264.7 cell and mouse models.
authors: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China.
authors: Na
authors: N
authors: Chen
authors: Dianfeng
authors: D
authors: Liu
authors: Lanan Wassy
authors: LW
authors: Soromou
authors: Jingjing
authors: J
authors: Sun
authors: Weiting
authors: W
authors: Zhong
authors: Weixiao
authors: W
authors: Guo
authors: Meixia
authors: M
authors: Huo
authors: Hongyu
authors: H
authors: Li
authors: Shuang
authors: S
authors: Guan
authors: Zhenwen
authors: Z
authors: Chen
authors: Haihua
authors: H
authors: Feng
mesh_terms: D000098:Acetophenones / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000097:blood / Q000276:immunology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008810:Mice, Inbred C57BL; D029596:Paeonia / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012772:Shock, Septic / Q000097:blood / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000098:Acetophenones; D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D008070:Lipopolysaccharides; C482199:lipopolysaccharide, E coli O55-B5; C013638:paeonol
keywords: cytokines; lipopolysaccharide; paeonol; pathway; survival