title: Impaired fluidity and oxidizability of HDL hydrophobic core and amphipathic surface in dyslipidemic men.
abstract: OBJECTIVE
  To examine and compare the composition, fluidity and oxidizability of HDL hydrophobic core and amphipathic surface of two groups of adult males (25kg/m²<BMI<30kg/m²), the former mixed dyslipidemic patients (MD) and the latter age- and BMI-matched healthy controls.
  
  
  RESULTS
  Pyrenyl-cholesteryl ester and pyrenyl-phosphatidylcholine, respectively incorporated in HDL core or surface were used for measuring both 2,2'-azobis-2-methyl-propanimidamide-dihydrochloride-induced peroxidation kinetics and fluidities of these regions. In comparison with the controls, MD HDL showed: a) higher free cholesterol to phospholipid ratio in surface and triacylglycerols to cholesteryl ester ratio in the core, b) higher malondialdehyde levels and lower alpha-tocopherol and beta-carotene to neutral lipid ratios, c) a more rigid surface and more fluid core, d) dramatically decreased lag-time and increased propagation rate of peroxidation kinetic in the core, but only an increased propagation rate on the surface.
  
  
  CONCLUSIONS
  These results suggest that better knowledge of the physical-chemical properties and oxidizability of HDL core and surface could contribute to better understanding of the mechanisms connecting HDL alteration to increased risk of CDV in MD.
authors: Department of Biomedical and Clinical Sciences L. Sacco-Faculty of Medicine and Surgery-University of Milan, Via G. B. Grassi, 74 - 20157 Milan, Italy. roberta.cazzola@unimi.it
authors: Roberta
authors: R
authors: Cazzola
authors: Erica
authors: E
authors: Cassani
authors: Michela
authors: M
authors: Barichella
authors: Benvenuto
authors: B
authors: Cestaro
mesh_terms: D000328:Adult; D015992:Body Mass Index; D002318:Cardiovascular Diseases / Q000453:epidemiology* / Q000209:etiology; D055598:Chemical Phenomena; D050171:Dyslipidemias / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007558:Italy / Q000453:epidemiology; D007700:Kinetics; D015227:Lipid Peroxidation; D008075:Lipoproteins, HDL / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008560:Membrane Fluidity; D008875:Middle Aged; D009765:Obesity / Q000150:complications*; D050177:Overweight / Q000150:complications*; D010084:Oxidation-Reduction; D012306:Risk; D013499:Surface Properties
chemical_list: D008075:Lipoproteins, HDL; D008315:Malondialdehyde