title: Molecular characterization of virulent Leptospira interrogans serogroup icterohaemorrhagiae isolated from Cavia aperea.
abstract: Leptospirosis is a worldwide zoonotic infection caused by pathogenic Leptospira. Synanthropic rodents are recognized carriers of leptospires; however, the role of wild rodents in the epidemiology of the disease is still incipient. In this work, we describe Leptospira strain isolated from Cavia aperea (Brazilian guinea pig). The isolated strain was characterized by partial rpoB gene sequencing, variable-number tandem-repeats and histopathological analysis. The strain was identified as Leptospira interrogans, serogroup Icterohaemorrhagiae and caused clinical signs of leptospirosis in the hamster model, attesting to its virulence. In conclusion, these findings could be useful for elucidating the epidemiological role of C. aperea in leptospirosis.
authors: Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Campus Universitário Capão do Leão, CP 354, CEP: 96010-900 Pelotas, RS, Brazil. leonardogmonte@hotmail.com
authors: Leonardo G
authors: LG
authors: Monte
authors: Sérgio
authors: S
authors: Jorge
authors: Marina A
authors: MA
authors: Xavier
authors: Fernanda M A
authors: FM
authors: Leal
authors: Marta G
authors: MG
authors: Amaral
authors: Fabiana K
authors: FK
authors: Seixas
authors: Odir A
authors: OA
authors: Dellagostin
authors: Cláudia P
authors: CP
authors: Hartleben
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D006224:Cricetinae; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005819:Genetic Markers; D006168:Guinea Pigs / Q000382:microbiology*; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D041383:Leptospira interrogans serovar icterohaemorrhagiae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D007922:Leptospirosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008647:Mesocricetus; D018598:Minisatellite Repeats / Q000235:genetics; D012376:Rodent Diseases / Q000453:epidemiology / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014774:Virulence; D015047:Zoonoses
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005819:Genetic Markers