title: 3'-Deoxy-3'-[(18)F]-fluorothymidine ([(18)F]-FLT) transport in newly diagnosed glioma: correlation with nucleoside transporter expression, vascularization, and blood-brain barrier permeability.
abstract: 3'-Deoxy-3'-[(18)F]-fluorothymidine ([(18)F]-FLT), a marker of cellular proliferation, has been used in positron emission tomography (PET) examination of gliomas. The aim of this study was to investigate whether the uptake of [(18)F]-FLT in glioma correlates with messenger RNA (mRNA) levels of the equilibrative nucleoside transporter 1 (ENT1), microvascular density (assessed by CD34 immunohistochemistry), and the blood-brain barrier (BBB) breakdown. A total of 21 patients with newly diagnosed glioma were examined with [(18)F]-FLT PET. Tumor lesions were identified as areas of focally increased [(18)F]-FLT uptake, exceeding that of surrounding normal tissue. Dynamic analysis of [(18)F]-FLT PET revealed correlations between the phosphorylation rate constant k 3 and ENT1 expression; however there was no correlation between the kinetic parameters and CD34 score. There was a good correlation between the gadolinium (Gd) enhancement score (evaluating BBB breakdown) and ENT1 expression, CD34 score, and Ki-67 index. This preliminary study suggests that ENT1 expression might not reflect accumulation of [(18)F]-FLT in vivo due to BBB permeability in glioma.
authors: Department of Neurological Surgery, Kagawa University Faculty of Medicine, 1750-1 Ikenobe, Miki, Kagawa, 761-0173, Japan, ashinomi@med.kagawa-u.ac.jp.
authors: Aya
authors: A
authors: Shinomiya
authors: Keisuke
authors: K
authors: Miyake
authors: Masaki
authors: M
authors: Okada
authors: Takehiro
authors: T
authors: Nakamura
authors: Nobuyuki
authors: N
authors: Kawai
authors: Yoshio
authors: Y
authors: Kushida
authors: Reiji
authors: R
authors: Haba
authors: Nobuyuki
authors: N
authors: Kudomi
authors: Masaaki
authors: M
authors: Tokuda
authors: Takashi
authors: T
authors: Tamiya
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000378:metabolism*; D001932:Brain Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000378:metabolism*; D015224:Dideoxynucleosides / Q000378:metabolism*; D033721:Equilibrative Nucleoside Transporter 1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005462:Fluorine Radioisotopes / Q000378:metabolism*; D005910:Glioma / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D049268:Positron-Emission Tomography; D012333:RNA, Messenger / Q000378:metabolism; D019275:Radiopharmaceuticals / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D015224:Dideoxynucleosides; D033721:Equilibrative Nucleoside Transporter 1; D005462:Fluorine Radioisotopes; D012333:RNA, Messenger; D019275:Radiopharmaceuticals; C002854:alovudine