title: SODs, DNA binding and cleavage studies of new Mn(III) complexes with 2-((3-(benzyloxy)pyridin-2-ylimino)methyl)phenol.
abstract: Newly synthesized ligand [2-((3-(benzyloxy)pyridin-2-ylimino)methyl)phenol] (Bpmp) react with manganese(II) to form mononuclear complexes [Mn(phen)(Bpmp)(CH3COO)(H2O)]·4H2O (1), (phen=1,10-phenanthroline) and [Mn(Bpmp)2(CH3COO)(H2O)]·5H2O (2). These complexes were characterized by elemental analysis, IR, (1)H NMR, Mass, UV-vis spectral studies. Molar conductance and thermogravimetric analysis of these complexes were also recorded. The in vitro SOD mimic activity of Mn(III) complexes were carried out and obtained with good result. The DNA-binding properties of the complexes 1 and 2 were investigated by UV-spectroscopy, fluorescence spectroscopy and viscosity measurements. The spectral results suggest that the complexes 1 and 2 can bind to Calf thymus DNA by intercalation mode. The cleavage properties of these complexes with super coiled pUC19 have been studied using the gel electrophoresis method, wherein both complexes 1 and 2 displayed chemical nuclease activity in the absence and presence of H2O2 via an oxidative mechanism. All the complexes inhibit the growth of both Gram positive and Gram negative bacteria to competent level. The MIC was determined by microtiter method.
authors: Department of Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India.
authors: L
authors: L
authors: Shivakumar
authors: K
authors: K
authors: Shivaprasad
authors: Hosakere D
authors: HD
authors: Revanasiddappa
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology; D008345:Manganese / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D009181:Mycoses / Q000188:drug therapy; D010618:Phenanthrolines / Q000737:chemistry* / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology; D011725:Pyridines / Q000737:chemistry / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D056831:Coordination Complexes; D007364:Intercalating Agents; D010618:Phenanthrolines; D010636:Phenols; D011725:Pyridines; D008345:Manganese; D004247:DNA; C427203:calf thymus DNA; D013482:Superoxide Dismutase; C025205:1,10-phenanthroline