title: Mammalian two-hybrid assays for studies of interaction of p300 with transcription factors.
abstract: The two-hybrid system is a powerful genetic assay that allows the interaction between two proteins to be detected in vivo. It was originally described in 1989 and since then it has been one of the main techniques used to identify interactions between proteins from different cellular organisms. Here we describe the methods to study the interaction of p300 with other transcription factors, specifically between p300 and two transcription factors related to hypoxia and inflammation, HIF-1α and NF-κB-p65, respectively.
authors: Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors: Daniela B
authors: DB
authors: Mendonça
authors: Gustavo
authors: G
authors: Mendonça
authors: Lyndon F
authors: LF
authors: Cooper
mesh_terms: D015687:Cell Hypoxia; D002460:Cell Line; D003001:Cloning, Molecular; D004926:Escherichia coli; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007249:Inflammation; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D051996:Transcription Factor RelA / Q000378:metabolism*; D015533:Transcriptional Activation; D014162:Transfection; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D050880:p300-CBP Transcription Factors / Q000378:metabolism*
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C498143:RELA protein, human; D051996:Transcription Factor RelA; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly; D050880:p300-CBP Transcription Factors