title: Histone deacetylase class II and acetylated core histone immunohistochemistry in human brains with Huntington's disease.
abstract: OBJECTIVE
  Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder, caused by a CAG/polyglutamine repeat expansion, which is associated with a dysregulation of histone function and an impairment of protein transcription. Histone deacetylase (HDAC) inhibitors, such as vorinostat (SAHA), have shown promise as therapeutic agents. However, there have been few studies on the expression of HDACs and acetylated core histones (AcHs) in either normal animals or humans, or in HD patients or HD animal models. Therefore, we investigated the expression of HDACs and AcHs in HD brain by immunohistochemistry, and have compared findings with elderly control subjects and patients with frontotemporal lobar degeneration (FTLD) to determine whether any observed changes were specific for HD.
  
  
  CONCLUSIONS
  we show specific and significant losses of AcH2A, AcH2B, AcH3 and AcH4 expression from cells in the caudate nucleus and Purkinje cells of the cerebellum in HD compared to patients with FTLD and control subjects, while the level of HDAC 5 was increased in these cells.
authors: Wolfson Molecular Imaging Centre, School of Cancer and Enabling Sciences, University of Manchester, Manchester, UK.
authors: Hsin Hsien
authors: HH
authors: Yeh
authors: Daniel
authors: D
authors: Young
authors: Juri G
authors: JG
authors: Gelovani
authors: Andrew
authors: A
authors: Robinson
authors: Yvonne
authors: Y
authors: Davidson
authors: Karl
authors: K
authors: Herholz
authors: David M A
authors: DM
authors: Mann
mesh_terms: D000107:Acetylation; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000378:metabolism*; D005260:Female; D056464:Histone Deacetylase 2 / Q000032:analysis / Q000096:biosynthesis*; D006657:Histones / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
chemical_list: D006657:Histones; D056464:Histone Deacetylase 2