title: First detection of Waddlia chondrophila in Africa using SYBR Green real-time PCR on veterinary samples.
abstract: Waddlia chondrophila is a strict intracellular microorganism belonging to the order Chlamydiales that has been isolated twice from aborted bovine fetuses, once in USA and once in Germany. This bacterium is now considered as an abortigenic agent in cattle. However, no information is available regarding the presence of this bacterium in Africa. Given the low sensitivity of cell culture to recover such an obligate intracellular bacterium, molecular-based diagnostic approaches are warranted. This report describes the development of a quantitative SYBR Green real-time PCR assay targeting the recA gene of W. chondrophila. Analytical sensitivity was 10 copies of control plasmid DNA per reaction. No cross-amplification was observed when testing pathogens that can cause abortion in cattle. The PCR exhibited a good intra-run and inter-run reproducibility. This real-time PCR was then applied to 150 vaginal swabs taken from Tunisian cows that have aborted. Twelve samples revealed to be Waddlia positive, suggesting a possible role of this bacterium in this setting. This new real-time PCR assay represents a diagnostic tool that may be used to further study the prevalence of Waddlia infection.
authors: Unité de recherche Toxicologie - Microbiologie Environnementale et Santé (UR11ES70), Faculté des Sciences de Sfax, Université de Sfax, Sfax, Tunisia.
authors: Mohamed
authors: M
authors: Barkallah
authors: Imen
authors: I
authors: Fendri
authors: Amina
authors: A
authors: Dhieb
authors: Yaakoub
authors: Y
authors: Gharbi
authors: Gilbert
authors: G
authors: Greub
authors: Radhouane
authors: R
authors: Gdoura
mesh_terms: D037881:Aborted Fetus; D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002695:Chlamydiales / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014416:Tunisia