title: Localized micro-scale disruption of cells using laser-generated focused ultrasound.
abstract: We utilize laser-generated focused ultrasound (LGFU) to create targeted mechanical disturbance on a few cells. The LGFU is transmitted through an optoacoustic lens that converts laser pulses into focused ultrasound. The tight focusing (<100 µm) and high peak pressure of the LGFU produces cavitational disturbances at a localized spot with micro-jetting and secondary shock-waves arising from micro-bubble collapse. We demonstrate that LGFU can be used as a non-contact, non-ionizing, high-precision tool to selectively detach a single cell from its culture substrate. Furthermore, we explore the possibility of biomolecule delivery in a small population of cells targeted by LGFU at pressure amplitudes below and above the cavitation threshold. We experimentally confirm that cavitational disruption is required for delivery of propidium iodide, a membrane-impermeable nucleic acid-binding dye, into cells.
authors: Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Hyoung Won
authors: HW
authors: Baac
authors: John
authors: J
authors: Frampton
authors: Jong G
authors: JG
authors: Ok
authors: Shuichi
authors: S
authors: Takayama
authors: L Jay
authors: LJ
authors: Guo
mesh_terms: D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002477:Cells / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D055595:Mechanical Phenomena; D014465:Ultrasonics
keywords: cavitation; cell detachment; drug delivery; laser-generated focused ultrasound