title: Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes.
abstract: In nature, enzymes often form multienzyme complexes to enhance their catalytic efficiencies and, -moreover, evolve into genetically fused multidomain enzymes. Inspired by a natural fusion cytochrome P450 (P450) containing a monooxygenase domain and a reductase domain, we have developed a heterotrimeric protein-utilized method to form a multienzyme complex composed of a bacterial P450 and its catalytically essential two redox proteins. Three distinct proliferating cell nuclear antigens (PCNAs) from Sulfolobus solfataricus, each of which can be separately expressed, spontaneously form a heterotrimer. Fusion to the PCNAs enables complex formation of a bacterial P450 and two redox proteins through the self-assembling of the PCNAs and enhances the activity due to efficient electron transfer in the complex. This PCNA-mediated multienzyme complex formation will be available for other multienzyme reactions.
authors: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.
authors: Hidehiko
authors: H
authors: Hirakawa
authors: Teruyuki
authors: T
authors: Nagamune
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D009097:Multienzyme Complexes / Q000737:chemistry*; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry* / Q000235:genetics; D021122:Protein Subunits / Q000737:chemistry*; D048229:Sulfolobus solfataricus / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D018809:Proliferating Cell Nuclear Antigen; D021122:Protein Subunits; D003577:Cytochrome P-450 Enzyme System