title: The effect of human chorionic gonadotropin treatment on recipient mouse germ cell proliferation following spermatogonial stem cell transplantation of neonatal donor mice.
abstract: Spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. The present study was designed to examine the effect of hCG treatment on germ cell proliferation following stem cell transplantation in mice. Spermatogonial stem cells were isolated from neonatal mice testes and characterized by alkaline phosphatase, immunoreactivity and morphological analysis. hCG was injected into normal and cell transplanted mice. We then evaluated the testosterone levels and cell number in normal mice. After that, cyclin B1 gene expression was investigated in transplanted mice. Different doses of busulfan were injected to investigate the effects of chemotherapy on morphological criteria and preparation of recipient mice for transplantation. In this report we show proliferative potential of spermatogonial stem cells after cytotoxic treatment, transplantation efficiency by semi-quantitative RT-PCR, and hCG effect on stem cell regeneration in normal mice and following cell transplantation. The results indicate that spermatogonial stem cells can proliferate after transplantation, and the efficiency of their transplantation depends on hormonal treatment. Therefore, hormonal treatment after stem cell transplantation will be a powerful avenue for increasing the efficiency of transplantation and fertility restoration.
authors: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran ; These authors equally contribute to this work.
authors: Mohammad Mehdi
authors: MM
authors: Akhondi
authors: Reza Akbarzadeh
authors: RA
authors: Najar
authors: Mahmood
authors: M
authors: Jeddi-Tehrani
authors: Mohammad-Reza
authors: MR
authors: Sadeghi
authors: Amir Hassan
authors: AH
authors: Zarnani
authors: Hodjattallah
authors: H
authors: Rabbani
authors: Sheida
authors: S
authors: Salehkhou
authors: Leila
authors: L
authors: Eini
authors: Fatemeh
authors: F
authors: Hoseinzadeh
authors: Mahnaz
authors: M
authors: Heidari
keywords: Busulfan; Cell proliferation; Transplantation; hCG