title: Thinopyrum 7Ai-1-derived small chromatin with Barley Yellow Dwarf Virus (BYDV) resistance gene integrated into the wheat genome with retrotransposon.
abstract: Thinopyrum intermedium is a useful source of resistance genes for Barley Yellow Dwarf Virus (BYDV), one of the most damaging wheat diseases. In this study, wheat/Th. intermedium translocation lines with a BYDV resistance gene were developed using the Th. intermedium 7Ai- 1 chromosome. Genomic in situ hybridization (GISH), using a Th. intermedium total genomic DNA probe, enabled detection of 7Ai-1-derived small chromatins containing a BYDV resistance gene, which were translocated onto the end of wheat chromosomes in the lines Y95011 and Y960843. Random amplified polymorphic DNA (RAPD) analyses using 120 random 10-mer primers were conducted to compare the BYDV-resistant translocation lines with susceptible lines. Two primers amplified the DNA fragments specific to the resistant line that would be useful as molecular markers to identify 7Ai-1-derived BYDV resistance chromatin in the wheat genome. Additionally, the isolated Th. intermedium-specific retrotransposon-like sequence pTi28 can be used to identify Th. intermedium chromatin transferred to the wheat genome.
authors: Molecular Genetics Laboratory, Faculty of Agriculture Tottori University, Japan.
authors: Y-Z
authors: YZ
authors: Ma
authors: M
authors: M
authors: Tomita
mesh_terms: D002843:Chromatin; D032461:Chromosomes, Plant; D017931:DNA Primers; D018014:Gene Transfer Techniques; D005819:Genetic Markers / Q000276:immunology; D018745:Genome, Plant; D017403:In Situ Hybridization; D017793:Luteovirus / Q000276:immunology*; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D018626:Retroelements; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000821:virology
chemical_list: D002843:Chromatin; D017931:DNA Primers; D005819:Genetic Markers; D018626:Retroelements