title: [Determination of kinds of monoamine neurotransmitters in Alzheimer's blood plasma by high performance liquid chromatography].
abstract: OBJECTIVE
  A method detecting norepinephrine, epinephrine, dopamineand serotonin in Alzheimer's blood plasma by high performance liquid chromatography was developed.
  
  
  METHODS
  C18 column was used, the mobile phase was composed of methanol-water (0.1 mol/ml KH2PO4) (3:97), pH 3.7. The flow rate was 0.8 ml/min, fluorescence detection wave length lambdaex was 290 nm and lambdaem was 330 nm, column temperature was room temperature.
  
  
  RESULTS
  The detection limits were 0.02 nmol/ ml for NE, 0.01 nmol/ml for E, 0.04 nmol/ml for DA, and 0. 02 nmol/ml for 5-HT. RSD was in the range of 2.2% - 4.4%. The recoveries of all analytes were in the range of 82.9% - 93.6%. The linear range was 0.02 - 2.0 nmol/ml for NE, 0.01 - 3.3 nmol/ml for E, 0.04 - 3.6 nmol/ml for DA, and 0.02 - 1.6 nmol/ml for 5-HT. The related coefficient was in the range of 0.9977 - 0.9985.
  
  
  CONCLUSIONS
  The assay had been applied successfully to measure the contents of these four monoamines in blood plasma of the Alzheimer's patients.
authors: College of Public Health, Harbin Medical University, Harbin 150081, China. wxh19851003@sina.com
authors: Xinhong
authors: X
authors: Wang
authors: Xiaohong
authors: X
authors: Sun
authors: Xiuqin
authors: X
authors: Liu
authors: Weiwei
authors: W
authors: Ma
authors: Wei
authors: W
authors: Xie
authors: Chao
authors: C
authors: Wang
authors: Decai
authors: D
authors: Wang
mesh_terms: D000544:Alzheimer Disease / Q000097:blood*; D015306:Biogenic Monoamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000097:blood; D004837:Epinephrine / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D018377:Neurotransmitter Agents / Q000097:blood*; D009638:Norepinephrine / Q000097:blood
chemical_list: D015306:Biogenic Monoamines; D018377:Neurotransmitter Agents; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine