title: Cross-reactivity of the anti-human D-dimer monoclonal antibody 1C9-6F10 to canine fibrin degradation products.
abstract: The cross-reactivity of the anti-human D-dimer antibody 1C9-6F10 to the canine D-dimer was evaluated by western blotting using purified canine fibrinogen, fibrin degradation products (XDPs) and plasma from a dog with suspected disseminated intravascular coagulation (DIC). The antibody cross-reacted with canine XDPs and plasma from the dog with suspected DIC. A latex agglutination assay using 1C9-6F10 measured the canine XDPs in dogs. In conclusion, the antibody 1C9-6F10 cross-reacted to the canine D-fragment with high specificity, but lower affinity compared with the human D-dimer. The latex agglutination assay using the 1C9-6F10 antibody was used to measure canine plasma D-dimer concentrations.
authors: Laboratory of Diagnostic Imaging, Department of Veterinary Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
authors: Naoki
authors: N
authors: Miura
authors: Makoto
authors: M
authors: Furukawa
authors: Yasuo
authors: Y
authors: Magari
authors: Yasuyuki
authors: Y
authors: Momoi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western / Q000662:veterinary; D003429:Cross Reactions / Q000276:immunology; D004211:Disseminated Intravascular Coagulation / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology*; D006801:Humans; D007841:Latex Fixation Tests / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D005338:Fibrin Fibrinogen Degradation Products