title: A mechanism-based pharmacological evaluation of efficacy of Trigonella foenum graecum (fenugreek) seeds in regulation of dyslipidemia and oxidative stress in hyperlipidemic rats.
abstract: : Alcoholic extract of Trigonella foenum graecum seeds [fenugreek seed extract (FSE)] was studied in triton-induced and high-fat diet-induced hyperlipidemia to evaluate antidyslipidemic effect. Plasma cholesterol (26.19%) and triglycerides (36.6%) were found to be lowered by FSE maximum at a dose of 200 mg/kg body weight in triton-treated hyperlipidemic rats. Chronic feeding of FSE (200 mg/kg body weight) caused lowering in plasma and hepatic lipid levels by activating lecithin-cholesterol acyltransferase (47%), postheparin lipolytic activity (35%), triglyceride lipase (34%), lipoprotein lipase (20.8%), and increased excretion of fecal bile acids (36%-45%). The FSE shows potent antioxidant activity in both in vitro and in vivo systems. It inhibited generation of superoxide anion and hydroxyl free radicals in both enzymatic and nonenzymatic systems significantly at 200 µM concentration. Furthermore, FSE normalizes the activities of antioxidant enzymes, that is, superoxide dismutase and catalase, and reduces plasma lipid peroxidation (33.9%), hepatic 4-hydroxynonenal (27%), and isoprostanes (28%). Data of the present study demonstrated that the T. foenum graecum seed extract has both antidyslipidemic and antioxidant properties.
authors: Departments of Biochemistry and Toxicology, Council of Scientific and Industrial Research, Central Drug Research Institute, New Delhi, India. .
authors: Upma
authors: U
authors: Chaturvedi
authors: Atul
authors: A
authors: Shrivastava
authors: Smriti
authors: S
authors: Bhadauria
authors: Jitendra K
authors: JK
authors: Saxena
authors: Gitika
authors: G
authors: Bhatia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000517:prevention & control*; D006949:Hyperlipidemias / Q000517:prevention & control*; D066298:In Vitro Techniques; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012639:Seeds / Q000737:chemistry; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood; D029910:Trigonella / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001647:Bile Acids and Salts; D010936:Plant Extracts; D014280:Triglycerides; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D008071:Lipoprotein Lipase