title: Analysis of genetic diversity in Larix gmelinii (Pinaceae)  with RAPD and ISSR markers.
abstract: Dahurian larch (Larix gmelinii), a deciduous  conifer, is the northernmost tree, native to eastern Siberia and nearby regions  of China. We used growth traits and molecular markers to assess genetic  variation in different L. gmelinii growing regions; 105 individual  samples were collected from seven regions of the Qingshan Forestry Centre,  Heilongjiang Province, China. The greatest genetic regional variation was seen  in the Youhao area, based on coefficients of variation for tree height,  diameter and volume (14.73, 28.25, and 55.27%, respectively). Analysis using  molecular markers showed rich genetic diversity. The RAPD and ISSR methods both  indicated that most variation came from within populations. The seven regions  were divided into two groups (Daxing'an and Xiaoxing'an Mountain ranges) by  RAPD cluster analysis: Tianchi, Xiaojiuya, Yuanjiang, and Taiping regions were  placed in the first group at a genetic distance of 0.08; while the other  regions were in the second group. The correlation between RAPD markers and  geographical distance was significant, with a correlation coefficient of 0.752.
authors: State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.
authors: L
authors: L
authors: Zhang
authors: H G
authors: HG
authors: Zhang
authors: X F
authors: XF
authors: Li
mesh_terms: D015415:Biomarkers; D002681:China; D017343:Genes, Plant; D014644:Genetic Variation; D005843:Geography; D028221:Larix / Q000235:genetics*; D018895:Microsatellite Repeats; D028201:Pinaceae / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
chemical_list: D015415:Biomarkers