title: Assessment of burden of virus agents in an urban sewage treatment plant in Rio de Janeiro, Brazil.
abstract: Sewage discharge is considered to be the main source of virus contamination in aquatic environments. There is no correlation between the presence of viruses and the presence of fecal coliforms in water; therefore virological markers are needed when monitoring contamination. This study investigates DNA and RNA virus concentrations in wastewater and evaluates a potential virus marker of human contamination. Influent and effluent samples were collected twice a month throughout a 1-year period. Viruses were detected using quantitative polymerase chain reaction protocols; nucleotide sequencing was carried out for virus genotyping. Human adenovirus (HAdV) and polyomavirus JC (JCPyV) were the most prevalent viruses found in influent samples (100%) with a virus load that ranged from 10(6) to 10(5) genome copies per liter (gc l(-1)). Norovirus genogroup II (NoV GII) and human astrovirus (HAstV) were less prevalent, and ranged from 10(4) to 10(3)gc l(-1). Quantitative data on virus profiles in wastewaters stress the high level of rotavirus species A environmental dissemination and address the potential of HAdV as a useful virological marker of virus contamination in aquatic environments. This study corroborates other studies performed in developed countries on DNA viruses as good markers of human fecal contamination.
authors: Laboratório de Virologia Comparada e Ambiental, Pavilhão Hélio & Peggy Pereira, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil, 4365-Manguinhos 21040-360 Rio de Janeiro, RJ, Brazil. tuliomf@ioc.fiocruz.br
authors: Tulio Machado
authors: TM
authors: Fumian
authors: Carmen Baur
authors: CB
authors: Vieira
authors: José Paulo Gagliardi
authors: JP
authors: Leite
authors: Marize Pereira
authors: MP
authors: Miagostovich
mesh_terms: D001938:Brazil; D002947:Cities; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012722:Sewage / Q000821:virology*; D014780:Viruses / Q000145:classification* / Q000302:isolation & purification*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology / Q000592:standards*; D018508:Water Purification / Q000379:methods
chemical_list: D012722:Sewage