title: Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay.
abstract: Naegleria spp. is a free-living amoeba that can be found in various aquatic environments. There are some Naegleria spp. that can cause fatal infections in animals and humans, and the most important source of infection is through direct water contact. In this study, a real-time quantitative PCR was developed to detect and quantify the Naegleria spp. in various environmental water samples. The water samples were taken from rivershed, water treatment plants, and thermal spring recreation areas. The total detection rate was 4.0% (7/176) for Naegleria spp. The percentages of samples containing Naegleria spp. from river water, raw drinking water, and thermal spring water were 0% (0/100), 10.7% (3/28) and 8.3% (4/48), respectively. The concentration of Naegleria spp. in detected positive raw drinking water and thermal spring water samples was in the range of 3.9-12.6 and 1.1-24.2 cells/L, respectively. The identified species included Naegleria australiensis, Naegleria lovaniensis, and Naegleria spitzbergeniensis. The presence of Naegleria spp. in various aquatic environments is considered a potential public health threat.
authors: Department of Surgery, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan, Republic of China.
authors: Po-Min
authors: PM
authors: Kao
authors: Min-Che
authors: MC
authors: Tung
authors: Bing-Mu
authors: BM
authors: Hsu
authors: Ming-Yuan
authors: MY
authors: Chou
authors: Hsiu-Wu
authors: HW
authors: Yang
authors: Cheng-Yu
authors: CY
authors: She
authors: Shu-Min
authors: SM
authors: Shen
mesh_terms: D009253:Naegleria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D059208:Parasite Load / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology