title: MiR-125b reduces porcine reproductive and respiratory syndrome virus replication by negatively regulating the NF-κB pathway.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus that has been devastating the swine industry worldwide since the late 1980s. To investigate the impact of cellular microRNAs (miRNAs) on the replication of PRRSV, we screened 10 highly conserved miRNAs implicated in innate immunity or antiviral function and identified miR-125b as an inhibitor of PRRSV replication. Virus titer and western blot assays demonstrated that miR-125b reduced PRRSV replication and viral gene expression in a dose-dependent manner in both MARC-145 cell line and primary porcine alveolar macrophages. Mechanistically, miR-125b did not target the PRRSV genome. Rather, it inhibited activation of NF-κB, which we found to be required for PRRSV replication. PRRSV, in turn, down-regulated miR-125b expression post-infection to promote viral replication. Collectively, miR-125b is an antiviral host factor against PRRSV, but it is subject to manipulation by PRRSV. Our study reveals an example of manipulation of a cellular miRNA by an arterivirus to re-orchestrate host gene expression for viral propagation and sheds new light on targeting host factors to develop effective control measures for PRRS.
authors: Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors: Dang
authors: D
authors: Wang
authors: Lu
authors: L
authors: Cao
authors: Zheng
authors: Z
authors: Xu
authors: Liurong
authors: L
authors: Fang
authors: Yao
authors: Y
authors: Zhong
authors: Quangang
authors: Q
authors: Chen
authors: Rui
authors: R
authors: Luo
authors: Huanchun
authors: H
authors: Chen
authors: Kui
authors: K
authors: Li
authors: Shaobo
authors: S
authors: Xiao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D023061:Gene Order; D016679:Genome, Viral; D000882:Haplorhini; D007372:Interferons / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000821:virology; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D016328:NF-kappa B / Q000378:metabolism*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D015398:Signal Transduction; D013552:Swine; D014779:Virus Replication
chemical_list: D035683:MicroRNAs; D016328:NF-kappa B; D007372:Interferons