title: Scanning ion conductance microscopy measurement of paracellular channel conductance in tight junctions.
abstract: Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental technique, potentiometric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular transport processes at high spatial resolution. The technique is validated for well-defined polymer membranes and then employed to study wild type and claudin-deficient mutants of Madin-Darby Canine Kidney strain II (MDCKII) cells. Paracellular permeabilities conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent conductance at subcellular levels.
authors: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, United States.
authors: Chiao-Chen
authors: CC
authors: Chen
authors: Yi
authors: Y
authors: Zhou
authors: Celeste A
authors: CA
authors: Morris
authors: Jianghui
authors: J
authors: Hou
authors: Lane A
authors: LA
authors: Baker
mesh_terms: D000818:Animals; D002460:Cell Line; D062446:Claudin-2 / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004848:Epithelium / Q000378:metabolism*; D004867:Equipment Design; D017136:Ion Transport; D007477:Ions / Q000378:metabolism*; D008853:Microscopy / Q000295:instrumentation*; D010539:Permeability; D019108:Tight Junctions / Q000378:metabolism*
chemical_list: D062446:Claudin-2; D007477:Ions