title: Hemagglutination mediated by the spike protein of cell-adapted bovine torovirus.
abstract: Bovine torovirus (BToV)-Aichi, recently isolated in cultured cells, showed hemagglutination (HA) activity, although the virus has a truncated hemagglutinin-esterase (HE) protein, judging from its gene structure, indicating the existence of another viral protein with HA activity. We examined whether the spike (S) protein possesses HA activity. A BToV antiserum used in this study, reactive to S but not to HE, inhibited HA activity. Furthermore, cells infected with BToV and those expressing S showed hemadsorption (HAD) activity, which was inhibited by the anti-BToV serum; however, HAD activity by expressed HE was not blocked. These data indicate that the S protein of BToV-Aichi is responsible for its HA activity.
authors: Laboratory of Virology and Viral Infections, Department of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyounan-chou, Musashino, Tokyo, Japan.
authors: Kozue
authors: K
authors: Shimabukuro
authors: Makoto
authors: M
authors: Ujike
authors: Toshihiro
authors: T
authors: Ito
authors: Hiroshi
authors: H
authors: Tsunemitsu
authors: Hitoshi
authors: H
authors: Oshitani
authors: Fumihiro
authors: F
authors: Taguchi
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000821:virology; D006384:Hemagglutination; D008562:Membrane Glycoproteins / Q000378:metabolism*; D064370:Spike Glycoprotein, Coronavirus; D017003:Torovirus / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D037521:Virulence Factors; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus