title: The conserved set of host proteins incorporated into HIV-1 virions suggests a common egress pathway in multiple cell types.
abstract: HIV-1 incorporates a large array of host proteins into virions. Determining the host protein composition in HIV virions has technical difficulties, including copurification of microvesicles. We developed an alternative purification technique using cholesterol that differentially modulates the density of virions and microvesicles (density modification, DM) allowing for high-yield virion purification that is essential for tandem mass spectrometric and quantitative proteomic (iTRAQ) analysis. DM purified virions were analyzed using iTRAQ and validated against Optiprep (60% iodixanol) purified virions. We were able to characterize host protein incorporation in DM-purified HIV particles derived from CD4+ T-cell lines; we compared this data set to a reprocessed data set of monocyte-derived macrophages (MDM) derived HIV-1 using the same bioinformatics pipeline. Seventy-nine clustered proteins were shared between the MDM derived and T-cell derived data set. These clusters included an extensive collection of actin isoforms, HLA proteins, chaperones, and a handful of other proteins, many of which have previously been documented to interact with viral proteins. Other proteins of note were ERM proteins, the dynamin domain containing protein EH4, a phosphodiesterase, and cyclophilin A. As these proteins are incorporated in virions produced in both cell types, we hypothesize that these proteins may have direct interactions with viral proteins or may be important in the viral life cycle. Additionally, identified common set proteins are predicted to interact with >1000 related human proteins. Many of these secondary interacting proteins are reported to be incorporated into virions, including ERM proteins and adhesion molecules. Thus, only a few direct interactions between host and viral proteins may dictate the host protein composition in virions. Ultimately, interaction and expression differences in host proteins between cell types may drive virion phenotypic diversity, despite conserved viral protein-host protein interactions between cell types.
authors: Graduate Program in Immunology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors: Michael E
authors: ME
authors: Linde
authors: David R
authors: DR
authors: Colquhoun
authors: Ceereena
authors: C
authors: Ubaida Mohien
authors: Thomas
authors: T
authors: Kole
authors: Veronica
authors: V
authors: Aquino
authors: Robert
authors: R
authors: Cotter
authors: Nathan
authors: N
authors: Edwards
authors: James E K
authors: JE
authors: Hildreth
authors: David R
authors: DR
authors: Graham
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002784:Cholesterol / Q000737:chemistry; D016000:Cluster Analysis; D015497:HIV-1 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D020543:Proteome; D002784:Cholesterol