title: Reversal effect of rosmarinic acid on multidrug resistance in SGC7901/Adr cell.
abstract: Multidrug resistance (MDR) has been a major problem in cancer chemotherapy. In this study, the aim was to explore the reversal effect and its potential mechanism of rosmarinic acid (RA) on SGC7901/Adr cells. 3-(4,5-Dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to investigate the reversal index of RA in SGC7901/Adr cell line. The intracellular accumulation of adriamycin, rhodamine123 (Rh123), and the expression of P-glycoprotein (P-gp) were assayed by flow cytometry. The influence of RA on the transcription of MDR1 gene was determined by reverse transcription-polymerase chain reaction. The results showed that RA could reverse the MDR of SGC7901/Adr cells, increase the intracellular accumulation of Adr and Rh123, and decrease the transcription of MDR1 gene and the expression of P-gp in SGC7901/Adr cells. These results indicated that RA was a potential multidrug resistance-reversing agent and warranted further investigations.
authors: Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China.
authors: Fa-Rong
authors: FR
authors: Li
authors: Yan-Yan
authors: YY
authors: Fu
authors: Dan-Hong
authors: DH
authors: Jiang
authors: Zhen
authors: Z
authors: Wu
authors: Yang-Jing
authors: YJ
authors: Zhou
authors: Ling
authors: L
authors: Guo
authors: Zhong-Min
authors: ZM
authors: Dong
authors: Zhe-Zhi
authors: ZZ
authors: Wang
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000494:pharmacology; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics; D002934:Cinnamates / Q000494:pharmacology*; D053630:Depsides / Q000494:pharmacology*; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D013274:Stomach Neoplasms / Q000378:metabolism; D014750:Vincristine / Q000494:pharmacology; D000097123:Rosmarinic Acid
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002934:Cinnamates; D053630:Depsides; D004317:Doxorubicin; D014750:Vincristine; D000097123:Rosmarinic Acid