title: Wheat resistome in response to barley yellow dwarf virus infection.
abstract: Barley yellow dwarf virus (BYDV) caused one of the most serious virus diseases of wheat (Triticum aestivum) worldwide. The wheat-Thinopyrum intermedium translocation line YW642 carries BYDV resistance gene Bdv2. To explore resistant wheat resistome in response to BYDV infection, we used Affymetrix GeneChip® Wheat Genome Arrays to analyze transcriptomes of YW642 and its susceptible parent Zhong8601 at 12 and 72 h postinoculation with BYDV compared to mock-inoculated controls. Relative to mock-inoculated control, 335 defense-related transcripts were upregulated in BYDV-inoculated YW642, among which 70 were upregulated only in BYDV-inoculated YW642 but not in BYDV-inoculated Zhong8601 through clustering analysis. Additional analysis using BYDV-inoculated Zhong8601 as reference revealed that 59 defense-related transcripts were upregulated in BYDV-inoculated YW642. Comparison of these upregulated defense transcripts obtained via the two analysis ways indicated that 19 overlapped defense-related transcripts were highly expressed in BYDV-inoculated YW642 relative to BYDV-inoculated Zhong8601 and mock-inoculated YW642, which likely are significant factors in Bdv2-mediated defense response to BYDV. High expression of some resistance homologous genes, pathogen-associated molecular pattern-triggered immunity-related genes, ABC transporter genes, pathogenesis-related protein genes, and genes in reactive oxygen species, phospholipid signaling, and jasmonic acid-signaling may contribute to Bdv2-mediated defense response to BYDV.
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of the Agriculture Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors: Xindong
authors: X
authors: Wang
authors: Yan
authors: Y
authors: Liu
authors: Liang
authors: L
authors: Chen
authors: Dan
authors: D
authors: Zhao
authors: Xifeng
authors: X
authors: Wang
authors: Zengyan
authors: Z
authors: Zhang
mesh_terms: D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D017793:Luteovirus / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012333:RNA, Messenger