title: DNA damage and cytotoxicity in adult subjects with prediabetes.
abstract: Prediabetes (intermediate hyperglycemia) is a high-risk state for diabetes that is defined by higher than normal glycemic levels that are below the level required for a diagnosis of diabetes. Prediabetes is characterized by oxidative stress, yet the associated DNA damage and cytotoxicity remain unknown to date. Therefore, we evaluated the relationship between glycemic alterations, DNA damage and cytotoxicity in the lymphocytes of individuals with pre-diabetes. Fasting plasma glucose (FPG) and glycated hemoglobin (A1C) levels were quantified and used as inclusion criteria. Anthropometric parameters were also evaluated. The cytokinesis-block micronucleus cytome assay (CBMN Cyt) was used to evaluate DNA damage and cytotoxicity. FPG correlated with A1C (r=0.562, p=0.002). Because A1C is the best predictor of diabetes complications, the association between A1C and the evaluated variables was assessed. The waist-hip ratio correlated with A1C (p<0.01). Regarding DNA damage, the frequency of nucleoplasmic bridges correlated with A1C (p<0.05). Both apoptosis and necrosis correlated with A1C (p<0.05). The overall frequency of DNA damage and cytotoxicity also correlated with A1C (p<0.01). Additional studies evaluating cell cycle and cell death patterns in prediabetes are necessary.
authors: University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil.
authors: Camila Schreiner
authors: CS
authors: Pereira
authors: Patrícia
authors: P
authors: Molz
authors: Roberta Passos
authors: RP
authors: Palazzo
authors: Thiago Aley Brites
authors: TA
authors: de Freitas
authors: Sharbel Weidner
authors: SW
authors: Maluf
authors: Jorge André
authors: JA
authors: Horta
authors: Daniel
authors: D
authors: Prá
authors: Silvia Isabel Rech
authors: SI
authors: Franke
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D004249:DNA Damage; D005215:Fasting / Q000097:blood; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D011236:Prediabetic State / Q000097:blood* / Q000235:genetics*; D049629:Waist-Hip Ratio
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human