title: Efficient chemical synthesis of human complement protein C3a.
abstract: We report the total chemical synthesis of human C3a by one-pot native chemical ligation of three unprotected peptide segments, followed by efficient in vitro folding that yielded the anaphylatoxin C3a in high yield and excellent purity. Synthetic C3a was fully active and its crystal structure at 2.1 Å resolution showed 3 helices and a C-terminal turn motif.
authors: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia.
authors: Artin
authors: A
authors: Ghassemian
authors: Ching-I Anderson
authors: CI
authors: Wang
authors: Mei-Kwan
authors: MK
authors: Yau
authors: Robert C
authors: RC
authors: Reid
authors: Richard J
authors: RJ
authors: Lewis
authors: David P
authors: DP
authors: Fairlie
authors: Paul F
authors: PF
authors: Alewood
authors: Thomas
authors: T
authors: Durek
mesh_terms: D002118:Calcium / Q000378:metabolism; D015926:Complement C3a / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D017510:Protein Folding; D017434:Protein Structure, Tertiary
chemical_list: D010455:Peptides; D015926:Complement C3a; D002118:Calcium