title: The zinc-finger domain was essential for porcine reproductive and respiratory syndrome virus nonstructural protein-1α to inhibit the production of interferon-β.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) has caused one of the most economically devastating and pandemic diseases of swine. Previous studies have documented that PRRSV nonstructural protein-1α (nsp1α) was an interferon antagonist, but the mechanism by which nsp1α inhibited the interferon (IFN)-β production was unclear. Here, by site-directed mutagenesis of the predicted zinc-coordinating residues of the zinc-finger (ZF) domain of nsp1α or by deletion of the ZF domain of nsp1α, we explored whether the ZF domain was required for nsp1α to disrupt the IFN-β production. The results showed that both mutagenesis of the predicted zinc-coordinating residues of the ZF domain and deletion of the ZF domain made nsp1α lose its interferon antagonism activity. In conclusion, our present work indicated that the ZF domain of nsp1α was necessary for nsp1α to inhibit the IFN-β induction.
authors: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Republic of China.
authors: Xibao
authors: X
authors: Shi
authors: Xiaozhuan
authors: X
authors: Zhang
authors: Fangyu
authors: F
authors: Wang
authors: Li
authors: L
authors: Wang
authors: Songlin
authors: S
authors: Qiao
authors: Junqing
authors: J
authors: Guo
authors: Chunhui
authors: C
authors: Luo
authors: Bo
authors: B
authors: Wan
authors: Ruiguang
authors: R
authors: Deng
authors: Gaiping
authors: G
authors: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D000882:Haplorhini; D016899:Interferon-beta / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed / Q000379:methods; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D017361:Viral Nonstructural Proteins; D016899:Interferon-beta; D015032:Zinc