title: Leukocyte infiltration in lung, muscle, and liver after limb compression in rats.
abstract: Muscle crush injury is associated with systemic manifestations known as crush syndrome. A systemic inflammatory response syndrome may be triggered by isolated crush injury. Using myeloperoxidase (MPO) activity and plasma fatty acid composition, we investigated the inflammatory response in distant organs after isolated limb compression in rats. Male Wistar rats were submitted to 1h of hind limb compression by a latex ribbon. Myeloperoxidase activity was measured in muscle, liver, and lung at progressive times (1, 2 or 4h) after bandage release. Plasma fatty acid composition was evaluated as an indirect measure of oxidative stress. The liver and hind limb muscles showed a transient increase in MPO activity. Pulmonary MPO activity, otherwise, increased progressively throughout the study and reached statistically significant values at 4h when compared to all other groups (p<0.05). Plasma levels of unsaturated fatty acids decreased gradually after decompression (p<0.05 compared to controls after 4h). Blunt traumatic muscle compression was associated with rapid and transient muscle and liver inflammatory cell infiltration but otherwise, polymorphonuclear cells showed progressive aggregation in lungs. The plasmatic unsaturated index decreased throughout the 4h after muscle release. We demonstrated that limb compression was associated with oxidative stress and distant inflammatory responses. Progressive inflammatory cell infiltration in lungs could be related with the delayed systemic adverse responses found after crush injury.
authors: School of Medicine, University of São Paulo (USP), SP, Brazil; Department of Biosciences, Federal University of São Paulo, UNIFESP, SP, Brazil. Electronic address: moralsgarbi@yahoo.com.br.
authors: Mauricio Wanderley Moral
authors: MW
authors: Sgarbi
authors: Bomfim A
authors: BA
authors: Silva
authors: Carmem Maldonado
authors: CM
authors: Peres
authors: Tatiana Carolina
authors: TC
authors: Alba
authors: Rui
authors: R
authors: Curi
authors: Francisco Garcia
authors: FG
authors: Soriano
authors: Daniel Araki
authors: DA
authors: Ribeiro
authors: Irineu Tadeu
authors: IT
authors: Velasco