title: Neuroprotective effect of a caffeic acid derivative from Abacopteris penangiana.
abstract: BACKGROUND
  A new caffeic acid derivative, named (7'Z)-3-O-(3, 4-dihydroxyphenylethenyl)-caffeic acid (CADP), extracted from Abacopteris penangiana (Hook.) Ching.
  
  
  OBJECTIVE
  To elucidate the neuroprotective effect of CADP against H₂O₂-induced cytotoxicity in PC12 cells and D-galactose (D-gal)-induced neurotoxicity in mice brain.
  
  
  METHODS
  CADP was isolated from the methanol extract of the rhizomes of A. penangiana. In vitro, the protective effect of CADP (0.1-10 μM) against H₂O₂-induced oxidative damage on PC12 cells was investigated by a MTT assay. In vivo, behavioral tests and antioxidant enzymes measurements were performed to investigate the protective effect of intraperitoneal (i.p.) injection of CADP (5 or 10 mg/kg/day) for 2 weeks on D-gal-induced neurotoxicity in mice.
  
  
  RESULTS
  The results showed that CADP significantly attenuated cell toxicity in a dose-dependent manner, and the EC₅₀ value of CADP was 0.83 ± 0.02 μM. In vivo, it was found that CADP significantly improved the behavioral performance of D-gal-treated mice in both Morris water maze (MWM) test and step-down avoidance test. As compared with model group, CADP (5, 10 mg/kg/day) attenuated the decrease in superoxide dismutase (SOD) activities by 40.5 and 75.4%, respectively; attenuated the decrease in glutathione peroxidase (GPx) activities by 53.8 and 73.2%, respectively; attenuated the decrease in catalase activities by 12.0 and 53.3%, respectively; reduced the increased levels of malondialdehyde (MDA) by 38.6 and 79.9%, respectively.
  
  
  CONCLUSIONS
  The results suggested that CADP has significant neuroprotective effects which can be attributed to inhibiting the generation of free radical and enhancing the activity of endogenous antioxidant enzymes.
authors: Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors: Wei
authors: W
authors: Fu
authors: Guang
authors: G
authors: Du
authors: Dong
authors: D
authors: Liu
authors: Jin-Lan
authors: JL
authors: Ruan
mesh_terms: D000375:Aging; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D002109:Caffeic Acids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D002681:China; D020834:Ethnopharmacology; D029624:Ferns / Q000737:chemistry*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D018697:Nootropic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D016716:PC12 Cells; D011897:Random Allocation; D051381:Rats; D027343:Rhizome / Q000737:chemistry
chemical_list: D002109:Caffeic Acids; D018696:Neuroprotective Agents; D018697:Nootropic Agents; C548196:caffeic acid 3,4-dihydroxyphenethyl ester; D010088:Oxidoreductases