title: Simultaneous determination of aflatoxin B(1) and ochratoxin A in licorice roots and fritillary bulbs by solid-phase extraction coupled with high-performance liquid chromatography-tandem mass spectrometry.
abstract: An effective method was developed for screening licorice roots and fritillary bulbs for contamination by aflatoxin B(1) and ochratoxin A using high-performance liquid chromatography connected to tandem mass spectrometry (HPLC-MS/MS). The samples were pre-concentrated and purified using solid-phase extraction, which provided satisfactory results. The separation was performed on a Waters Xbridge(TM) C18 column with a linear gradient of acetonitrile - water containing 5mM ammonium acetate. The MS spectrum was acquired in positive mode with both single quadrupole (Q1) and multiple reaction monitoring (MRM). The optimised method offered a good linear correlation (r(2)>0.9967), excellent precision (RSD<2.83%) and acceptable recovery (from 92.78 to 105.37%). The limits of detection (LOD) and the limits of quantification (LOQ) were less than 0.024 μg/kg and 0.095 μg/kg, respectively. The validated method was successfully applied to the rapid screening for AFB(1) and OTA in licorice roots and fritillary bulbs.
authors: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors: Lizhi
authors: L
authors: Wang
authors: Zhen
authors: Z
authors: Wang
authors: Weiwei
authors: W
authors: Gao
authors: Juan
authors: J
authors: Chen
authors: Meihua
authors: M
authors: Yang
authors: Ying
authors: Y
authors: Kuang
authors: Linfang
authors: L
authors: Huang
authors: Shilin
authors: S
authors: Chen
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D031410:Fritillaria / Q000737:chemistry*; D006035:Glycyrrhiza / Q000737:chemistry*; D057230:Limit of Detection; D009793:Ochratoxins / Q000032:analysis* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A; D016604:Aflatoxin B1