title: The correlation between microvessel pathological changes of the endplate and degeneration of the intervertebral disc in diabetic rats.
abstract: In this study, the pathological microvessel changes to the endplate and the degeneration of the intervertebral disc of diabetic rats were examined in order to identify the possible mechanism by which diabetes mellitus (DM) induces degeneration of the intervertebral disc. A total of 30 Sprague-Dawley rats were randomly divided into two groups. DM was induced in one of the groups by streptozotocin (STZ) administration. The rats were sacrificed 4, 8 and 12 weeks later. Five rats from each group were sacrificed at each time interval and lumbar disc and endplate tissue were obtained from each rat. The histological changes, collagen expression, microvessel density (MVD) and apoptosis of the disc were investigated by different methods. The expression of collagen I in the diabetic DM group was higher compared to the control group at the three time points (P<0.01), in contrast to the expression of collagen II. The factor VIII-related antigen (FVIII RAg) was expressed in the control and DM groups, while its expression was relatively low in the DM group. The MVD of the DM group was smaller compared to that of the control group at the three time points (P<0.01). The apoptotic index (AI) in the diabetic group was significantly higher compared to that of the control group at the three time points (P<0.01). A negative correlation was observed between the MVD of the endplates and the notochordal cell AI in the two groups. Compared to the control group, the endplate MVD decreased and the cavity became smaller or disappeared in the diabetic rats. In conclusion, there was a negative correlation between MVD and degenerative changes of the intervertebral disc in diabetic rats.
authors: Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060; ; Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008,  P.R. China.
authors: Sen
authors: S
authors: Chen
authors: Meimei
authors: M
authors: Liao
authors: Jianping
authors: J
authors: Li
authors: Hao
authors: H
authors: Peng
authors: Min
authors: M
authors: Xiong
keywords: diabetes mellitus; intervertebral disc; microvessel