title: Generation and characterization of KsprtTA and KsptTA transgenic mice.
abstract: The advent of technologies that allow tissue specific expression or ablation of genes has contributed enormously to our knowledge of the mechanism regulating organ development and maintenance in mice. The tetracycline inducible system allows reversible regulation of gene products upon administration of doxycycline. Here we describe the generation and activity of two transgenic lines expressing the cDNAs for the Tet responsive transcription factors rtTA and tTA (Tet-on and off) respectively under the control of an element that drives expression in the epithelium of the developing and adult kidney. Both lines show inducible and reversible activity in the embryonic and adult organ.
authors: Department of Internal Medicine (Nephrology), UT Southwestern Medical Center, Dallas, Texas 75390-9148, USA.
authors: Xinchao
authors: X
authors: Pan
authors: Erin V
authors: EV
authors: Small
authors: Peter
authors: P
authors: Igarashi
authors: Thomas J
authors: TJ
authors: Carroll
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009399:Nephrons / Q000378:metabolism*; D009924:Organ Culture Techniques; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation
chemical_list: D015820:Cadherins; C491946:Cdh16 protein, mouse; C045073:Cre recombinase; D019426:Integrases; D004318:Doxycycline