title: Multipotent adult progenitor cell-loaded demineralized bone matrix for bone tissue engineering.
abstract: Multipotent adult progenitor cells (MAPCs) from bone marrow have been shown to be capable of forming bone, cartilage and other connective tissues. In addition, MAPCs differentiate into lineages that are different from their germ layers of origin. Previous studies showed the ability of MAPCs to improve cardiac function and control allogenic-reactive responses associated with acute graft versus host disease. In the current study, we evaluated the ability of MAPCs to produce bone matrix on demineralized bone allograft substrates. Specifically, MAPCs expressed alkaline phosphatase, produced extracellular matrix proteins and deposited calcium-containing mineral on demineralized bone matrices. Furthermore, the addition of MAPCs on demineralized bone matrix (DBM) scaffolds enhanced osteoinductivity of the carrier in a rat ectopic pouch model. These results demonstrated the potential of MAPCs as a new approach for bone repair in tissue-engineering applications.
authors: Biotechnology Development Department, RTI Biologics, Alachua, FL, USA.
authors: Peter
authors: P
authors: Supronowicz
authors: Biotechnology Development Department, RTI Biologics, Alachua, FL, USA.
authors: Elise
authors: E
authors: Gill
authors: Biotechnology Development Department, RTI Biologics, Alachua, FL, USA.
authors: Angelica
authors: A
authors: Trujillo
authors: Biotechnology Development Department, RTI Biologics, Alachua, FL, USA.
authors: Taili
authors: T
authors: Thula
authors: Sports Medicine Group, RTI Biologics, Alachua, FL, USA.
authors: Rasa
authors: R
authors: Zhukauskas
authors: Athersys, Inc., Cleveland, OH, USA.
authors: Robert
authors: R
authors: Perry
authors: Biotechnology Development Department, RTI Biologics, Alachua, FL, USA.
authors: Ronald R
authors: RR
authors: Cobb
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000378:metabolism*; D001842:Bone and Bones / Q000502:physiology*; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D049109:Cell Proliferation; D002828:Choristoma / Q000473:pathology; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D016348:Osseointegration; D010012:Osteogenesis; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015415:Biomarkers; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: bone; mineralization; multipotent adult progenitor cells; tissue engineering