title: Broadly Neutralizing Antibody Responses in a Subset of HIV-1-Infected Individuals in Chennai, India.
abstract: Identification of broadly neutralizing antibodies (NAbs) generated during the course of HIV-1 infection is essential for effective HIV-1 vaccine design. The magnitude and breadth of neutralizing activity in the sera from 46 antiretroviral treatment-naive HIV-1 clade C-infected individuals was measured in a single round infection assay using TZM-bl cells and multisubtype panel of env-pseudotyped viruses. Higher levels of NAb response (NAb titer 500 to >40 000) were measured in these patients against tier 1 and tier 2 viruses. The average magnitude of the NAb responses of chronically infected individuals against heterologous viruses was consistently higher than the response observed from individuals with long-term nonprogressor ( P = .086). To conclude, high titers of HIV-1 cross-neutralizing activity were observed in the sera from a subset of HIV-1-infected individuals in Chennai, India. Additional studies of the epitopes recognized by these antibodies may facilitate the discovery of an effective vaccine immunogen.
authors: 1 YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India.
authors: Syed Iqbal
authors: SI
authors: Hussain
authors: 1 YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India.
authors: Nandagopal
authors: N
authors: Panneerselvam
authors: 1 YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India.
authors: Suniti
authors: S
authors: Solomon
authors: 1 YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India.|2 Johns Hopkins School of Medicine, Baltimore, MD, USA.
authors: Sunil S
authors: SS
authors: Solomon
authors: 3 Dartmouth College, Hanover, NH, USA.
authors: Kaavya
authors: K
authors: Adam
authors: 1 YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India.
authors: Ezhilarasi
authors: E
authors: Chandrasekaran
authors: 4 Duke University Medical Center, Durham, NC, USA.
authors: David C
authors: DC
authors: Montefiori
authors: 1 YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India.
authors: Balakrishnan
authors: B
authors: Pachamuthu
mesh_terms: D000328:Adult; D057134:Antibodies, Neutralizing / Q000097:blood*; D018791:CD4 Lymphocyte Count; D015331:Cohort Studies; D003429:Cross Reactions; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000453:epidemiology* / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests; D012367:RNA, Viral / Q000097:blood
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D012367:RNA, Viral
keywords: HIV-1 neutralization assay; HIV-1 pseudoviruses; LTNP; humoral response