title: Identifying health effects of exposure to trichloroacetamide using transcriptomics and metabonomics in mice ( Mus musculus ).
abstract: Microarray-based transcriptomics and one-dimensional proton nuclear magnetic resonance ((1)H NMR) based metabonomics approaches were employed to investigate the health effects of nitrogenous disinfection byproducts (N-DBPs) of trichloroacetamide (TCAcAm) on mice. Mice were exposed to TCAcAm at concentrations of 50, 500, and 5000 μg/L for 90 days, and hepatic transcriptome and serum metabonome and histopathological parameters were detected in comparison with those of control. TCAcAm esposures resulted in liver inflammation, weight loss (in 5000 ug/L TCAcAm group), and alterations in hepatic transcriptome and serum metabonome. Based on the differentially expressed genes and altered metabolites, several significant pathways were identified, which are associated with lipid, xenobiotics, amino acid and energy metabolism, and cell process. Moreover, integrative pathway analyses revealed that TCAcAm exposure in this study induced hepatotoxicity and cytotoxicity. These results also highlight the noninvasive prospect of transcriptomic and metabonomic approaches in evaluating the health risk of emerging N-DBPs.
authors: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, China.
authors: Yan
authors: Y
authors: Zhang
authors: Zongyao
authors: Z
authors: Zhang
authors: Yanping
authors: Y
authors: Zhao
authors: Shupei
authors: S
authors: Cheng
authors: Hongqiang
authors: H
authors: Ren
mesh_terms: D000081:Acetamides / Q000009:adverse effects*; D000818:Animals; D062845:Chloroacetates / Q000009:adverse effects*; D004202:Disinfectants / Q000009:adverse effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D051379:Mice; D044967:Serum / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects*; D015431:Weight Loss / Q000187:drug effects
chemical_list: D000081:Acetamides; D062845:Chloroacetates; D004202:Disinfectants; C009528:trichloroacetamide