title: Proteins of duck influenza virus responsible for acquisition of pathogenicity in chickens.
abstract: Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus by 3 consecutive passages in chickens from low pathogenic strain rgVac1-P0. Comparison of amino acid sequences of the virus proteins and experimental infection of chickens with a series of recombinant viruses demonstrated that in addition to the HA, each of the PA, NP, M1, and M2 of Vac1-P3 are responsible for the acquisition of pathogenicity in chickens. These 4 proteins of Vac1-P3 synergistically contributed to efficient virus replication in chickens.
authors: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Sapporo, Hokkaido 060-0818, Japan.
authors: Naoki
authors: N
authors: Yamamoto
authors: Kosuke
authors: K
authors: Soda
authors: Yoshihiro
authors: Y
authors: Sakoda
authors: Masatoshi
authors: M
authors: Okamatsu
authors: Hiroshi
authors: H
authors: Kida
mesh_terms: D000818:Animals; D002645:Chickens; D004372:Ducks; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D057916:Influenza A Virus, H7N1 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000473:pathology / Q000821:virology*; D016865:Reassortant Viruses / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D037521:Virulence Factors