title: Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice.
abstract: The purpose of this study was to analyze the influence of E- and P-selectins on the migratory pattern of magnetically labeled multipotent mesenchymal stromal cells (MSCs) in mice using magnetic resonance imaging (MRI). Murine MSCs were labeled with fluorescent iron oxide microparticles and carboxyfluorescein succinidyl ester (CFSE). Expression of common MSC markers, CD44, CD90, CD105, and Sca-1, as well as of selectin ligands, CD15s and CD162, was assessed using flow cytometry and immunocytochemistry. Labeled MSCs were injected into E-/P-selectin-deficient and wild-type mice applying doses of 5 × 10(4) cells intracardially, 1 × 10(6) cells intravenously, and 5 × 10(6) cells intraperitoneally. After cell administration, mice underwent MRI repeatedly and histologic evaluation after 7 days. The results demonstrate that magnetically labeled murine MSCs retain their expression of the above-mentioned surface markers and their ability to interact with P-selectin. Furthermore, MRI examinations and histologic analysis revealed that E-/P-selectin deficiency in mice significantly alters the distribution of labeled MSCs after cell injection via different routes.
authors: Department of Diagnostic and Interventional Radiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. k.peldschus@uke.uni-hamburg.de
authors: Kersten
authors: K
authors: Peldschus
authors: Johannes
authors: J
authors: Salamon
authors: Daniel
authors: D
authors: Wicklein
authors: Claudia
authors: C
authors: Lange
authors: Harald
authors: H
authors: Ittrich
authors: Gerhard
authors: G
authors: Adam
authors: Udo
authors: U
authors: Schumacher
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019040:E-Selectin / Q000378:metabolism*; D005434:Flow Cytometry; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D019007:P-Selectin / Q000378:metabolism*
chemical_list: D019040:E-Selectin; D019007:P-Selectin; D007501:Iron