title: A study on the suitability of inactivated Trichinella spiralis larvae for proficiency samples.
abstract: The consumption of raw or undercooked Trichinella infected meat, especially pork and horse meat, can have important implications for public health. Therefore each animal carcass from a Trichinella susceptible species intended for human consumption must be examined for Trichinella. Laboratories carrying out testing of official control samples must undergo a quality assurance program and should regularly participate in proficiency testing schemes. To date, Trichinella proficiency samples are prepared with live larvae, which, as a level 2 pathogen, require specific shipping and disinfection procedures. Therefore, the suitability of using inactivated Trichinella larvae as proficiency samples was tested. We found that Trichinella larvae treated with 2% formaldehyde for 24h had lost their infectivity and showed a comparable recovery rate to naïve larvae after artificial digestion, albeit with a prolonged sedimentation time.
authors: Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, 12277 Berlin, Germany. anne.mayer-scholl@bfr.bund.de
authors: A
authors: A
authors: Mayer-Scholl
authors: S
authors: S
authors: Reckinger
authors: K
authors: K
authors: Nöckler
mesh_terms: D000818:Animals; D003257:Consumer Product Safety; D004063:Digestion; D005506:Food Contamination / Q000032:analysis*; D005513:Food Inspection / Q000379:methods* / Q000592:standards; D016266:Food Parasitology; D005557:Formaldehyde; D006801:Humans; D059021:Laboratory Proficiency Testing; D007814:Larva; D008460:Meat / Q000469:parasitology*; D011786:Quality Control; D013552:Swine; D017160:Trichinella spiralis / Q000254:growth & development / Q000302:isolation & purification*; D014235:Trichinellosis / Q000469:parasitology*
chemical_list: D005557:Formaldehyde