title: Single-molecule methods for studying gene regulation in vivo.
abstract: The recent emergence of new experimental tools employing sensitive fluorescence detection in vivo has made it possible to visualize various aspects of gene regulation at the single-molecule level in the native, intracellular context. In this review, we will first describe general considerations for in vivo, single-molecule fluorescence detection of DNA, mRNA, and protein molecules involved in gene regulation. We will then give an overview of the rapidly evolving suite of molecular tools available for observing gene regulation in vivo and discuss new insights they have brought into gene regulation.
authors: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21211, USA.
authors: Zach
authors: Z
authors: Hensel
authors: Jie
authors: J
authors: Xiao
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D005456:Fluorescent Dyes