title: [Detection of oral fungal flora in patients with primary Sjogren's syndrome by real-time PCR].
abstract: OBJECTIVE
  To compare the quantity of the main important Candida species in the oral cavity between the patients with primary Sjogren's syndrome (pSS) and no dry mouth healthy controls,and to explore the discriminative species of fungi between the two groups.
  
  
  METHODS
  In this study, 25 pSS patients from Department of Oral Medicine of Peking University School and Hospital of Stomatology were enrolled, with 25 residents from a community in Beijing as the control group. All the participants were examined for the oral mucosa status, and 5 Candida DNA loads were compared between the two groups by use of the FQ-PCR technique.
  
  
  RESULTS
  Candida albicans was the dominant Candida in both of the groups. In addition, we found that the species of Candida tropicalis and Parapsilosis were more detected in the pSS group by use of the FQ-PCR technique. Quantitative of Candida tropicalis and Candida parapsilosis in pSS group (10.6 ± 1.07, 9.47 ± 4.86) was significantly higher than that in healthy controls group (8.30 ± 3.82, 5.24 ± 6.05), the difference was statistically significant (P <0.05). Quantitative of fluconazole-sensitive strains in pSS group (12.21 ± 0.82) compared with which in healthy controls group (11.53 ± 0.81) was significantly increased (P <0.01).
  
  
  CONCLUSIONS
  Candida albicans was the dominant Candida in both of the groups. Candida tropicalis and Candida parapsilosis were more detected in the pSS group.
authors: Department of Oral Medicine, Peking University School, Beijing, China.
authors: Ning
authors: N
authors: Ding
authors: Zhi-min
authors: ZM
authors: Yan
authors: Hong
authors: H
authors: Hua
mesh_terms: D000368:Aged; D002175:Candida / Q000145:classification / Q000302:isolation & purification; D016022:Case-Control Studies; D005260:Female; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology*; D012859:Sjogren's Syndrome / Q000382:microbiology*