title: Protein expression in the salivary glands of dengue-infected Aedes aegypti mosquitoes and blood-feeding success.
abstract: Mosquito salivary glands (SG) play an essential role in food digestion and pathogen transmission. The function of the salivary components during infection is poorly understood. In this study, female Aedes aegypti mosquitoes were infected with dengue virus serotype 2 (DENV-2) via an artificial membrane feeding apparatus. The mosquito SGs were examined for DENV-2 infection for 14 days post-infection (dpi). The amount of dengue virus increased throughout the 14 dpi. Three different meals were provided for the Ae. aegypti mosquitoes. SG protein expression was compared among sugar-fed (SF), blood-fed (BF), and dengue-infected blood-fed (DF) mosquitoes using SDS-PAGE coupled with densitometric analysis. The SG of SF mosquitoes had fewer protein bands than those of BF and DF mosquitoes. The major SG proteins seen among BF and DF mosquitoes had molecular weights of 12-15, 25-30, 35-40, 45-50, 55-60 kDa and 61-67 kDa. We compared the SG protein band expression profiles in BF and DF mosquitoes. Two bands (35-40 and 61-67 kDa) were expressed more by DF mosquitoes and 3 different bands (25-30, 45-50, and 55-60 kDa) were expressed more by BF mosquitoes. These SG proteins may have some role in facilitating blood-feeding and dengue infection. We speculate these specific SG proteins in dengue-infected mosquitoes may increase the chance of blood-feeding and virus transmission by infected mosquitoes. These results may be useful for designing additional tools to investigate the interaction between Ae. aegypti SG and the dengue virus.
authors: Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors: Ladawan
authors: L
authors: Wasinpiyamongkol
authors: Sirilaksana
authors: S
authors: Patramool
authors: Supatra
authors: S
authors: Thongrungkiat
authors: Pannamas
authors: P
authors: Maneekan
authors: Suntaree
authors: S
authors: Sangmukdanan
authors: Dorothée
authors: D
authors: Missé
authors: Natthanej
authors: N
authors: Luplertlop
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D004261:DNA Replication / Q000502:physiology; D003715:Dengue / Q000635:transmission* / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D011499:Protein Processing, Post-Translational; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000821:virology; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D013785:Thailand; D014779:Virus Replication / Q000502:physiology
chemical_list: D012471:Salivary Proteins and Peptides