title: The PI(3)P interactome from a colon cancer cell.
abstract: A comprehensive analysis of the phosphoinositide interactome has been performed using an ω-amino analogue of phosphatidylinositol 3-phosphate (PI(3)P immobilised onto Affi-10 beads for use as an affinity absorbent for cytosolic, membrane and nuclear extracts from the LIM1215 colonic carcinoma cell line. Affinity/LC/MS/MS experiments allowed the identification of 681 proteins/protein complexes which interact with PI(3)P. Protein domain enrichment analysis identified proteins possessing PI(3)P (e.g., FYVE, PX, PH), PIP and PIP/phospholipid binding domains along with small GTPases, GTPase regulators, kinases and SH2/SH3 containing proteins. Functional and pathway enrichment analyses highlighted the major role of PI(3)P in endocytosis dynamics and vesicular trafficking, intracellular cell signalling regulation, cell division and cytokinesis.
  
  
  CONCLUSIONS
  This study provides an initial detailed assessment of the phosphatidylinositol 3-phosphate (PI(3)P) interactome, highlights the major role of PI(3)P in endocytosis dynamics and vesicular trafficking, cell intracellular regulation, signalling and cytokinesis and suggests potential PI(3)P specificity for further biochemical and biological characterisation.
authors: Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Post Office Box 2008 Royal Melbourne Hospital, Victoria 3050, Australia. Bruno.catimel@ludwig.edu.au
authors: Bruno
authors: B
authors: Catimel
authors: Eugene
authors: E
authors: Kapp
authors: Meng-Xin
authors: MX
authors: Yin
authors: Mark
authors: M
authors: Gregory
authors: Leon S-M
authors: LS
authors: Wong
authors: Melanie
authors: M
authors: Condron
authors: Nicole
authors: N
authors: Church
authors: Nadia
authors: N
authors: Kershaw
authors: Andrew B
authors: AB
authors: Holmes
authors: Antony W
authors: AW
authors: Burgess
mesh_terms: D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D018129:Phosphatidylinositol Phosphates