title: DNA damage and oxidative stress in human B lymphoblastoid cells after combined exposure to hexavalent chromium and nickel compounds.
abstract: In the present study, human B lymphoblastoid cells were exposed to potassium dichromate and/or nickel chloride for 24h or 48h. The cell viability and DNA damage induced by these compounds was measured with the CCK-8 assay and Comet assay, respectively. In addition, the generation of reactive oxygen species (ROS) and the levels of malondialdehyde (MDA) were measured using commercially available kits. Our results indicated that potassium dichromate could decrease cell viability and induce DNA damage in human B lymphoblastoid cells in a time - and concentration - dependent manner, but the toxicity of nickel chloride was not so obvious at concentrations used in our study. The results of ROS showed that both two compounds could only induce weak elevation of ROS level, but MDA levels were significantly enhanced. Antagonistic effects of cytotoxicity were mainly found between Cr (VI) and Ni (II), and synergistic effects of DNA damage and oxidative stress were partially found between these two compounds. Moreover, there were good correlations between the results of comet assay and the results of oxidative stress assays. It is suggested that synergistic DNA damage induced by simultaneously exposure of hexavalent chromium and nickel compounds is possibly related to oxidative stress.
authors: Zhejiang Academy of Medical Sciences, Institute of Hygiene, Hangzhou 310013, Zhejiang, People's Republic of China. jianlinlou@163.com
authors: Jianlin
authors: J
authors: Lou
authors: Lingzhi
authors: L
authors: Jin
authors: Nanxiang
authors: N
authors: Wu
authors: Yufeng
authors: Y
authors: Tan
authors: Yang
authors: Y
authors: Song
authors: Ming
authors: M
authors: Gao
authors: Kecheng
authors: K
authors: Liu
authors: Xing
authors: X
authors: Zhang
authors: Jiliang
authors: J
authors: He
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002857:Chromium / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D009532:Nickel / Q000633:toxicity*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D002857:Chromium; D008315:Malondialdehyde; D009532:Nickel