title: Development of a new UPLC-MSMS method for the determination of temozolomide in mice: application to plasma pharmacokinetics and brain distribution study.
abstract: A sensitive and accurate liquid chromatography method with mass spectrometry detection was developed and validated for the quantification of temozolomide in mouse plasma and brain. Theophyllin was used as the internal standard. A single-step protein precipitation was used for plasma and brain sample preparation. The method was validated with respect to selectivity, extraction recovery, linearity, intra- and inter-day precision and accuracy, limit of quantification and stability. The method has a limit of quantification of 50 ng/mL for temozolomide in plasma and 125 ng/g in brain. This method was used successfully to perform brain and plasma pharmacokinetic studies of temozolomide in mice after intraperitoneal administration.
authors: Clinical Pharmacy Department - EA 4123, College of Pharmacy, Paris 11 University, 5 rue Jean Baptiste Clement, 92296, Chatenay Malabry, France. lauriane.goldwirt@u-psud.fr
authors: Lauriane
authors: L
authors: Goldwirt
authors: Noel
authors: N
authors: Zahr
authors: Robert
authors: R
authors: Farinotti
authors: Christine
authors: C
authors: Fernandez
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D016018:Least-Squares Analysis; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077204:Temozolomide; D014018:Tissue Distribution
chemical_list: D003606:Dacarbazine; D000077204:Temozolomide