title: Phylogenetic and phylogeographic analyses of porcine circovirus type 2 among pig farms in Vietnam.
abstract: This study demonstrated the prevalence of Porcine circovirus type 2 (PCV2) among pig farms in Vietnam. Analyses of the genome, capsid protein and phylogeny classified all 30 Vietnamese PCV2 strains as the PCV2b genotype, belonging to the clusters of 1A, 1B, 1C and recombinant forms. Each viral genome was 1767 nucleotides long and shared 96.0-100% nucleotide sequence identity. The amino acid substitutions in the capsid protein of the Vietnamese PCV2 strains were in immunodominant regions, and the majority of strains (24/30) contained a lysine extension at the C-terminus. Bayesian phylogeographic analysis revealed epidemic links of the PCV2 recombinant cluster within and among countries, which supports a circulating recombinant form of PCV2. Further analysis by the Jameson-Wolf antigenic index indicated antigenic alterations at important sites in the capsid protein (sites 131-133) among the recombinant cluster and the other clusters of PCV2b.
authors: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Hanoi University of Agriculture, Hanoi, Vietnam.
authors: T M L
authors: TM
authors: Huynh
authors: B H
authors: BH
authors: Nguyen
authors: V G
authors: VG
authors: Nguyen
authors: H A
authors: HA
authors: Dang
authors: T N
authors: TN
authors: Mai
authors: T H G
authors: TH
authors: Tran
authors: M H
authors: MH
authors: Ngo
authors: V T
authors: VT
authors: Le
authors: T N
authors: TN
authors: Vu
authors: T K C
authors: TK
authors: Ta
authors: V H
authors: VH
authors: Vo
authors: H K
authors: HK
authors: Kim
authors: B K
authors: BK
authors: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D036022:Capsid Proteins / Q000235:genetics; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral / Q000235:genetics; D005838:Genotype; D010802:Phylogeny; D058974:Phylogeography; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D014744:Vietnam / Q000453:epidemiology
chemical_list: D036022:Capsid Proteins
keywords: PCV2; Vietnam; phylogenetic; phylogeographic