title: Carbonic anhydrase-related protein XI: structure of the gene in the greater false vampire bat (Megaderma lyra) compared with human and domestic pig.
abstract: Carbonic anhydrase-related protein XI (CA-RP XI) is a member of the α-carbonic anhydrase family (encoded by the gene CA-11), which has lost features of the active site required for enzymatic activity. Using PCR, we amplified CA-11 from genomic DNA of the bat Megaderma lyra. To elucidate the gene structure, we sequenced PCR products and compared their sequences with genomic and mRNA sequences known from human and domestic pig. We identified and sequenced eight introns in the bat CA-11. Five introns (introns 3-7) are located in identical or similar positions in other members of the vertebrate α-carbonic anhydrase gene family. Two 5' introns and one 3' intron are located in the regions of little or no sequence similarity with other members of the gene family. The low sequence similarity and additional introns suggest a separate evolutionary origin for the 5' and 3' portions of the CA-RP XI gene.
authors: Department of Biology, Xavier University of Louisiana, New Orleans, LA 70125, USA. cporter@xula.edu
authors: Calvin A
authors: CA
authors: Porter
authors: David
authors: D
authors: Hewett-Emmett
authors: Richard E
authors: RE
authors: Tashian
mesh_terms: D000818:Animals; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics*; D002685:Chiroptera / Q000235:genetics*; D005091:Exons / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D002256:Carbonic Anhydrases