title: Purification and identification of lipolysis-stimulating peptides derived from enzymatic hydrolysis of soy protein.
abstract: The aim of this study was to purify and identify lipolysis-stimulating peptides derived from Flavourzyme®-soy protein isolate (SPI) hydrolysate (F-SPIH). Glycerol release was employed as a marker for lipolysis in 3T3-L1 adipocytes. A higher glycerol release represents a better lipolysis-stimulating activity. The peptide fraction with highest glycerol release obtained from F-SPIH fractionated by sequential ultrafiltration membranes was further purified using gel filtration chromatography and two steps of reverse-phase high-performance liquid chromatography. The peptides were identified using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Three lipolysis-stimulating peptides were obtained, and the amino acid sequences were ILL, LLL and VHVV, respectively. The in vitro effect of gastrointestinal proteases on lipolysis-stimulating activity of synthetic ILL, LLL and VHVV, respectively, was also investigated. The result suggested that the gastrointestinal protease did not affect lipolysis-stimulating activity of the three novel peptides, which reveals their potential to act as anti-obesity ingredients.
authors: Department of Culinary Arts, Chung Chou University of Science and Technology, 6 lane 3 Sec. 3 Shanchiao Rd., Yuanlin Changhua 51003, Taiwan, ROC.
authors: May-June
authors: MJ
authors: Tsou
authors: Fuh-Juin
authors: FJ
authors: Kao
authors: Hsi-Chi
authors: HC
authors: Lu
authors: Hao-Chun
authors: HC
authors: Kao
authors: Wen-Dee
authors: WD
authors: Chiang
mesh_terms: D000818:Animals; D006868:Hydrolysis; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D041681:NIH 3T3 Cells; D010447:Peptide Hydrolases / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000494:pharmacology; D030262:Soybean Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D011492:Protein Hydrolysates; D030262:Soybean Proteins; D010447:Peptide Hydrolases