title: Apoptosis induced by NS1 gene of Canine Parvovirus-2 is caspase dependent and p53 independent.
abstract: Apoptosis is programmed cell death that normally occurs during development and aging in multicellular animals. Apoptosis also occurs as a defense mechanism against disease or harmful external agents. It can be initiated by a variety of stimuli including viruses and viral proteins. Canine parvovirus type 2 (CPV-2) that causes acute disease in dogs has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. Though non structural protein 1 (NS1) of many parvoviruses has been found to be apoptotic, no report on the apoptotic potential of NS1 of CPV-2 (CPV-2.NS1) exists. In this study, we evaluated the apoptotic potential of CPV-2.NS1 in HeLa cells. CPV-2.NS1 has been found to induce apoptosis which was evident through characteristic DNA fragmentation, increase in hypodiploid cell count, phosphatidyl serine translocation and activation of caspase-3. Increase in caspase-3 activity and no change in p53 activity with time in CPV-2.NS1 expressing HeLa cells showed the induction of apoptosis to be caspase dependent and p53 independent.
authors: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors: Lovleen
authors: L
authors: Saxena
authors: G Ravi
authors: GR
authors: Kumar
authors: Shikha
authors: S
authors: Saxena
authors: Uttara
authors: U
authors: Chaturvedi
authors: Aditya Prasad
authors: AP
authors: Sahoo
authors: Lakshya Veer
authors: LV
authors: Singh
authors: Lakshman
authors: L
authors: Santra
authors: S K
authors: SK
authors: Palia
authors: G S
authors: GS
authors: Desai
authors: A K
authors: AK
authors: Tiwari
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D053938:DNA Fragmentation; D004847:Epithelial Cells / Q000502:physiology / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D017993:Parvovirus, Canine / Q000472:pathogenicity*; D010718:Phosphatidylserines / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D010718:Phosphatidylserines; D016159:Tumor Suppressor Protein p53; D017361:Viral Nonstructural Proteins; D037521:Virulence Factors; D053148:Caspase 3