title: Involvement of interleukin-17 during lymphocytic bronchiolitis in lung transplant patients.
abstract: BACKGROUND
  Interleukin-17 (IL-17) is involved in autoimmune and chronic pulmonary diseases and linked with neutrophilic inflammation. Azithromycin reduces and prevents broncholaveolar lavage (BAL) neutrophilia after lung transplantation (LTx). In this investigation we assessed the involvement of IL-17 in different post-transplant complications in human LTx biopsies.
  
  
  METHODS
  Immunohistochemical staining against IL-17A was performed on biopsies of LTx patients with either chronic rejection, acute A-grade rejection (A > 2B0), lymphocytic bronchiolitis (LB), infection, and stable patients. Biopsies of 7 patients with LB were stained before and after azithromycin treatment. IL-17+ cells were quantified as number per square millimeter of lamina propria. Double staining with CD4/CD8 was performed to determine the origin of IL-17.
  
  
  RESULTS
  In the LB group, biopsies showed a significant presence of IL-17+ cells/mm2 of lamina propria compared with the stable, acute A-grade/chronic rejection and infection groups (p < 0.0001). The number of IL-17+ cells on biopsy correlated with percent BAL (%BAL) neutrophilia (r = 0.34, p = 0.0056). Azithromycin reduced both %BAL neutrophilia and IL-17+ cells (both p = 0.016) in the LB group. CD8+ cells were the major source of IL-17.
  
  
  CONCLUSIONS
  IL-17+ / CD8+ cells are present in LB after LTx but not in acute A-grade/chronic rejection nor during infection. Moreover, azithromycin significantly decreased the number of IL-17+ cells in the airway wall, which may further explain its effect on BAL neutrophilia.
authors: Lung Transplant Unit, KU Leuven and University Hospital Gasthuisberg, Leuven, Belgium. stijn.verleden@med.kuleuven.be
authors: Stijn E
authors: SE
authors: Verleden
authors: Robin
authors: R
authors: Vos
authors: Elly
authors: E
authors: Vandermeulen
authors: David
authors: D
authors: Ruttens
authors: Annemie
authors: A
authors: Vaneylen
authors: Lieven J
authors: LJ
authors: Dupont
authors: Erik K
authors: EK
authors: Verbeken
authors: Geert M
authors: GM
authors: Verleden
authors: Dirk E
authors: DE
authors: Van Raemdonck
authors: Bart M
authors: BM
authors: Vanaudenaerde
mesh_terms: D000328:Adult; D017963:Azithromycin / Q000494:pharmacology; D001988:Bronchiolitis / Q000276:immunology*; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000502:physiology*; D016040:Lung Transplantation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects; D011183:Postoperative Complications / Q000276:immunology*; D012189:Retrospective Studies
chemical_list: D020381:Interleukin-17; D017963:Azithromycin