title: Identifying the viral genes encoding envelope glycoproteins for differentiation of Cyprinid herpesvirus 3 isolates.
abstract: Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies.
authors: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea. yellowjeeeun@hanmail.net
authors: Jee Eun
authors: JE
authors: Han
authors: Ji Hyung
authors: JH
authors: Kim
authors: Tristan
authors: T
authors: Renault
authors: Casiano
authors: C
authors: Choresca
authors: Sang Phil
authors: SP
authors: Shin
authors: Jin Woo
authors: JW
authors: Jun
authors: Se Chang
authors: SC
authors: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D006564:Herpesviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins