title: Identification of a novel polyomavirus from vervet monkeys in Zambia.
abstract: To examine polyomavirus (PyV) infection in wildlife, we investigated the presence of PyVs in Zambia with permission from the Zambia Wildlife Authority. We analysed 200 DNA samples from the spleens and kidneys (n = 100 each) of yellow baboons and vervet monkeys (VMs) (n = 50 each). We detected seven PyV genome fragments in 200 DNA samples using a nested broad-spectrum PCR method, and identified five full-length viral genomes using an inverse PCR method. Phylogenetic analysis of virally encoded proteins revealed that four PyVs were closely related to either African green monkey PyV or simian agent 12. Only one virus detected from a VM spleen was found to be related, with relatively low nucleotide sequence identity (74 %), to the chimpanzee PyV, which shares 48 % nucleotide sequence identity with the human Merkel cell PyV identified from Merkel cell carcinoma. The obtained entire genome of this virus was 5157 bp and had large T- and small t-antigens, and VP1 and VP2 ORFs. This virus was tentatively named vervet monkey PyV 1 (VmPyV1) as a novel PyV. Comparison with other PyVs revealed that VmPyV1, like chimpanzee PyV, had a longer VP1 ORF. To examine whether the VmPyV1 genome could produce viral proteins in cultured cells, the whole genome was transfected into HEK293T cells. We detected VP1 protein expression in the transfected HEK293T cells by immunocytochemical and immunoblot analyses. Thus, we identified a novel PyV genome from VM spleen.
authors: Global COE Program, Hokkaido University, N18, W9, Kita-ku, Sapporo 060-0818, Japan.|Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors: Hiroki
authors: H
authors: Yamaguchi
authors: Global COE Program, Hokkaido University, N18, W9, Kita-ku, Sapporo 060-0818, Japan.|Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors: Shintaro
authors: S
authors: Kobayashi
authors: Hokudai Center for Zoonosis Control in Zambia, PO Box 32379, Lusaka, Zambia.
authors: Akihiro
authors: A
authors: Ishii
authors: Hokudai Center for Zoonosis Control in Zambia, PO Box 32379, Lusaka, Zambia.
authors: Hirohito
authors: H
authors: Ogawa
authors: Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors: Ichiro
authors: I
authors: Nakamura
authors: Department of Paraclinical Studies, School of Veterinary and Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors: Ladslav
authors: L
authors: Moonga
authors: Department of Paraclinical Studies, School of Veterinary and Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors: Bernard M
authors: BM
authors: Hang'ombe
authors: Department of Disease Control, School of Veterinary and Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors: Aaron S
authors: AS
authors: Mweene
authors: Hokudai Center for Zoonosis Control in Zambia, PO Box 32379, Lusaka, Zambia.
authors: Yuka
authors: Y
authors: Thomas
authors: Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors: Takashi
authors: T
authors: Kimura
authors: Global COE Program, Hokkaido University, N18, W9, Kita-ku, Sapporo 060-0818, Japan.|Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors: Hirofumi
authors: H
authors: Sawa
authors: Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors: Yasuko
authors: Y
authors: Orba
mesh_terms: D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops / Q000821:virology*; D016679:Genome, Viral; D057809:HEK293 Cells; D006801:Humans; D007668:Kidney / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D027601:Polyomavirus Infections / Q000662:veterinary* / Q000821:virology; D018419:Primate Diseases / Q000821:virology*; D013154:Spleen / Q000821:virology; D015024:Zambia