title: DOPE facilitates quaternized lipidoids (QLDs) for in vitro DNA delivery.
abstract: This paper describes the synthesis of a combinatorial library of quaternized lipidoids (QLDs) and an evaluation of their abilities to facilitate in vitro DNA delivery. The QLDs alone showed low efficiency for DNA delivery. By formulating liposomes with a neutral helper lipid, such as 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), the capability of QLDs for gene transfection is significantly enhanced due to the fusogenic properties of DOPE which facilitate endosomal escape and cargo delivery. We further optimized the liposome composition and DNA dose for gene transfection and investigated the structure-activity relationships of the lipidoid library in DNA delivery.
  
  
  BACKGROUND
  This paper describes the synthesis and evaluation of a combinatorial library of quaternized lipidoids to facilitate in vitro DNA delivery, which occurs at a low level but can be enhanced with DOPE. The authors also further optimized the liposome composition and DNA dose for delivery and investigated the structure-activity relationships of the lipidoid library.
authors: Department of Chemical and Biological Engineering, Tufts University, Medford, MA.
authors: Shuo
authors: S
authors: Sun
authors: Ming
authors: M
authors: Wang
authors: Kyle A
authors: KA
authors: Alberti
authors: Alex
authors: A
authors: Choy
authors: Qiaobing
authors: Q
authors: Xu
mesh_terms: D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000378:metabolism*; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D014162:Transfection
chemical_list: D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA
keywords: Helper lipid; Non-viral gene delivery; Quaternized lipidoids