title: Quantification of L-ergothioneine in whole blood by hydrophilic interaction ultra-performance liquid chromatography and UV-detection.
abstract: A new hydrophilic interaction ultra-performance LC method was established for the whole blood measurement of L-ergothioneine. Chromatographic separation was achieved in a fairly short time, less than 4 min, on a 100 × 2.1 mm Acquity UPLC BEH HILIC 1.7 μm column with a mobile phase consisting of a mixture of 100 mmol/L ammonium acetate/ACN/water (5:85:10, v/v/v) that flowed isocratically at 0.250 mL/min. The LOD and the limit of quantification were 3.85 and 11.67 μmol/L, respectively. The method exhibited linearity in a concentration range of 15.63-1000 μmol/L (R(2) > 0.999). Mean recovery was 96.34% whereas intraassay and interassay precision were 1.52 and 1.82% RSD, respectively. On the whole, the developed method is simple, fast, precise, accurate, and sensitive and may be useful for routine analyses.
authors: Department of Biomedical Sciences, University of Sassari, Sassari, Italy. salvatore.sotgia@gmail.com
authors: Salvatore
authors: S
authors: Sotgia
authors: Angelo
authors: A
authors: Zinellu
authors: Gianfranco
authors: G
authors: Pintus
authors: Gerard Aime
authors: GA
authors: Pinna
authors: Luca
authors: L
authors: Deiana
authors: Ciriaco
authors: C
authors: Carru
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004880:Ergothioneine / Q000097:blood*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004880:Ergothioneine