title: Identification of a vibration regime favorable for bone healing and muscle in estrogen-deficient rats.
abstract: Numerous whole-body vibration (WBV) devices of various forces are available on the market, although their influence on the musculoskeletal system is not yet understood. The effect of different WBVs on bone healing and muscle function was evaluated in rats ovariectomized at 3 months of age. 2 months after ovariectomy, bilateral metaphyseal tibia osteotomy and T-plate osteosynthesis were performed. Rats were divided into groups: intact, OVX, and OVX exposed to vertical WBVs of 35, 50, 70, or 90 Hz (experiment 1) or horizontal WBVs of 30, 50, 70, or 90 Hz (experiment 2) 5 days after osteotomy (0.5 mm, 15 min/day for 30 days). The tibia and gastrocnemius and soleus muscles were collected. Vertical vibrations (>35 Hz) improved cortical and callus densities, enlarged callus area and width, suppressed the tartrate-resistant acid phosphatase gene, enhanced citrate synthase activity, accelerated osteotomy bridging (35 and 50 Hz), upregulated the osteocalcin (Oc) gene (70 Hz), and increased relative muscle weight (50 Hz). Horizontal vibrations reduced cortical width (<90 Hz) and callus density (30 Hz), enhanced alkaline phosphatase (Alp) gene expression (50 Hz), decreased the size of oxidative fibers (35 and 70 Hz), and increased capillary density (70, 90 Hz). Biomechanical data; serum Oc, Alp, and creatine kinase activities; body weight; and food intake did not change after WBVs. Vertical WBVs of 35 and 50 Hz produced more favorable results than the higher frequencies. Horizontal WBV showed no positive or negative effects. Further studies are needed to elucidate the effects of WBV on different physiological systems, and precautions must be taken when implementing WBV in the treatment of patients.
authors: Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Goettingen, Robert-Koch 40, 37075, Goettingen, Germany. komrakova@yahoo.com
authors: Marina
authors: M
authors: Komrakova
authors: Stephan
authors: S
authors: Sehmisch
authors: Mohammad
authors: M
authors: Tezval
authors: Jan
authors: J
authors: Ammon
authors: Peggy
authors: P
authors: Lieberwirth
authors: Cordula
authors: C
authors: Sauerhoff
authors: Lukas
authors: L
authors: Trautmann
authors: Michael
authors: M
authors: Wicke
authors: Christian
authors: C
authors: Dullin
authors: Klaus M
authors: KM
authors: Stuermer
authors: Ewa K
authors: EK
authors: Stuermer
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D005260:Female; D005593:Fracture Fixation, Internal; D017102:Fracture Healing / Q000502:physiology; D050723:Fractures, Bone / Q000534:rehabilitation*; D018482:Muscle, Skeletal / Q000502:physiology*; D010027:Osteotomy; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000502:physiology* / Q000601:surgery; D014732:Vibration / Q000627:therapeutic use*