title: Determination of ginsenoside Rc in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.
abstract: Ginsenoside Rc (GRc) is a potential pharmacologically active ingredient isolated from ginseng (Panax ginseng C.A. Meyer, Araliaceae). A simple, rapid and sensitive method for determination of GRc in rat plasma was developed based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte and internal standard (IS), ginsenoside Rb(1) (GRb(1)), were extracted from plasma with n-butanol and chromatographied on a C(18) column eluted with a mobile phase of methanol and water containing 0.1% formic acid. The detection was performed by positive ion electrospray ionization in selective reaction monitoring mode (SRM), monitoring the transitions m/z 1101.6→789.3 and m/z 1131.7→364.7 for GRc and IS, respectively. The assay was linear over the concentration range of 5-5000 ng/mL with a limit of quantitation (LOQ) of 5 ng/mL. The accuracy was between 86.7% and 114.9%, and the precision was less than 9.7%. This method was successfully applied to investigate the pharmacokinetic study of GRc in rats after intravenous (2mg/kg) and oral (20mg/kg) administration, and the result showed that the ginsenoside was poorly absorbed with an absolute bioavailability being approximately 0.17%.
authors: Tasly R&D Institute, Tianjin Tasly Group Co. Ltd., Tianjin 300410, China.
authors: Yang
authors: Y
authors: Chu
authors: Hong-chao
authors: HC
authors: Zhang
authors: Shu-ming
authors: SM
authors: Li
authors: Jun-mei
authors: JM
authors: Wang
authors: Xiang-yang
authors: XY
authors: Wang
authors: Wei
authors: W
authors: Li
authors: Lan-lan
authors: LL
authors: Zhang
authors: Xiao-hui
authors: XH
authors: Ma
authors: Shui-ping
authors: SP
authors: Zhou
authors: Yong-hong
authors: YH
authors: Zhu
authors: Chang-xiao
authors: CX
authors: Liu
mesh_terms: D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides; C044462:ginsenoside Rc