title: Autologous platelet-poor plasma decreases the bronchial stump necrosis in rat.
abstract: BACKGROUND
  Necrosis of the bronchial stump is a very important trigger for bronchopleural fistula. The administration of local autologous platelet-poor plasma (PPP) could protect the bronchial stump.
  
  
  METHODS
  Left pneumonectomy was performed in 25 Sprague-Dawley rats. Animals were randomly assigned to a control group (n=13) and PPP group (n=12). PPP was locally administered on the bronchial stump after pneumonectomy. We analyzed histologic changes in the bronchial stump and messenger RNA expression changes of genes involved in wound repair at 10 and 20 d.
  
  
  RESULTS
  Local PPP treatment produced a mass of fibrous tissue surrounding the bronchial stump and significantly decreased the presence of necrosis at 20 d. PPP increased the expression of insulin like growth factor 1 at 10 d although it did not reach statistical significance.
  
  
  CONCLUSIONS
  Our findings indicate that local PPP treatment of the bronchial stump after pneumonectomy decreased necrosis and could have a protective effect on the bronchial stump.
authors: Research Unit, Experimental Surgery, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain. norbesr@terra.es
authors: Norberto
authors: N
authors: Santana-Rodríguez
authors: Pedro
authors: P
authors: Llontop
authors: Bernardino
authors: B
authors: Clavo
authors: Rafael
authors: R
authors: Camacho
authors: Ardiel
authors: A
authors: Quintana
authors: María D
authors: MD
authors: Fiuza
authors: José Manuel
authors: JM
authors: García-Castellano
authors: Miguel A
authors: MA
authors: Ponce-González
authors: Keila
authors: K
authors: Zerecero
authors: Leandro
authors: L
authors: Fernández-Pérez
authors: Yanira
authors: Y
authors: Brito-Godoy
authors: José A
authors: JA
authors: Ruíz-Caballero
mesh_terms: D000818:Animals; D001804:Blood Transfusion, Autologous; D001980:Bronchi / Q000473:pathology*; D001983:Bronchial Fistula / Q000209:etiology / Q000517:prevention & control*; D015870:Gene Expression; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D009336:Necrosis / Q000209:etiology / Q000517:prevention & control; D010949:Plasma; D010995:Pleural Diseases / Q000209:etiology / Q000517:prevention & control*; D011013:Pneumonectomy / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: C548904:insulin-like growth factor-1, rat; D007334:Insulin-Like Growth Factor I