title: Panton-Valentine leukocidin positive sequence type 80 methicillin-resistant Staphylococcus aureus carrying a staphylococcal cassette chromosome mec type IVc is dominant in neonates and children in an Algiers hospital.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is a major antimicrobial drug-resistant pathogen causing serious infections. It was first detected in healthcare settings, but in recent years it has also become disseminated in the community. Children and young adults are most susceptible to infection by community-acquired (CA) MRSA strains. In this study 25 MRSA isolates implicated in infections of neonates and children admitted to an Algiers hospital during an 18 month period were characterized by molecular methods including staphylococcal cassette chromosome (SCC) mec typing, PCR amplification of pvl genes, pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Fifteen out of 25 isolates were from hospital-acquired infections. Twenty-four isolates carried SCCmec type IVc and belonged to the sequence type (ST) 80, one isolate carried SCCmec type II and was ST 39. Twenty-two out of 24 ST80-MRSA-IVc isolates carried pvl genes. Our results suggest that the Panton-Valentine leukocidin positive ST80- MRSA-IVc is the dominant MRSA clone causing disease in neonates and children in Algiers.
authors: Laboratory of Applied Microbiology, Faculty of Natural Sciences, A/MIRA University, Bejaia Algeria. djo.ferhat@gmail.com
authors: Ferhat
authors: F
authors: Djoudi
authors: Celestino
authors: C
authors: Bonura
authors: Said
authors: S
authors: Benallaoua
authors: Abdelaziz
authors: A
authors: Touati
authors: Djamila
authors: D
authors: Touati
authors: Aurora
authors: A
authors: Aleo
authors: Cinzia
authors: C
authors: Cala
authors: Teresa
authors: T
authors: Fasciana
authors: Caterina
authors: C
authors: Mammina
mesh_terms: D000293:Adolescent; D000462:Algeria; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000188:drug therapy / Q000382:microbiology; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D005098:Exotoxins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D007956:Leukocidins / Q000235:genetics* / Q000302:isolation & purification; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000235:genetics* / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin