title: Intracellular interaction of newly synthesized nerve growth factor and its receptors.
abstract: In autocrine cells, both a ligand and its receptors are synthesized in the same cell, but their intracellular interaction is not well known. We examined it using PC84 cells, a mutant PC12 cell line expressing nerve growth factor (NGF). We have already reported that the intracellular precursor of TrkA was phosphorylated and that MAP kinase was phosphorylated in PC84 cells. In this paper we found that the NGF receptors, TrkA and p75NTR, existed mainly as precursors, and most p75NTR localized inside PC84 cells. The phosphorylation of MAP kinase was also observed even when PC84 cells were incubated with anti-NGF antibody to block the extracellular interaction. These results suggest the possibility that newly synthesized NGF could interact intracellularly with the receptors in PC84 cells.
authors: Laboratory of Biochemistry, College of Pharmaceutical Sciences, Matsuyama University, Ehime 790-8578, Japan. hnomoto@cc.matsuyama-u.ac.jp
authors: Hiroshi
authors: H
authors: Nomoto
authors: Hisako
authors: H
authors: Notsu
authors: Yuka
authors: Y
authors: Kato
authors: Keigo
authors: K
authors: Ekinaga
authors: Shoei
authors: S
authors: Furukawa
mesh_terms: D000818:Animals; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D016716:PC12 Cells; D010766:Phosphorylation; D051381:Rats; D020917:Receptor, trkA / Q000096:biosynthesis / Q000378:metabolism*; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; C068313:Ngfr protein, rat; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020929:Mitogen-Activated Protein Kinase Kinases