title: Carbon tetrachloride-induced lipid peroxidation and hyperglycemia in rat: a novel study.
abstract: Launaea procumbens methanol extract (LPME) was evaluated against carbon tetrachloride (CCl4)-induced pancreatic oxidative damage and hyperglycemia in rats. Various doses of the extract were administered to rats after 48 h of CCl4 treatment (3 ml/kg bodyweight (bw); intraperitoneally, 20% CCl4/olive oil) twice a week for 4 weeks. Coadministration of various concentrations of LPME (100, 150 and 200 mg/kg) ameliorated the toxicity of CCl4 and reversed the serum level of enzymes (amylase and lipase), glucose and hormone (insulin). The extract was able to reduce thiobarbituric acid reactive substance but increased the glutathione contents in pancreatic tissue. Depletion of antioxidant enzyme activities (catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase and glutathione-S-reductase) and DNA damages induced with CCl4 were restored by LPME supplement. It is suggested that LPME effectively protects the liver against the CCl4-induced oxidative damage in rats, possibly through antioxidant and/or free radical scavenging effects of flavonoids and phenolic compounds in the extract.
authors: Faculty of Biological Sciences, Department of Biotechnology, University of Science and Technology Bannu, Khyber Pakhtunkhwa, Pakistan Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan rahmatgul_81@yahoo.com.
authors: Rahmat Ali
authors: RA
authors: Khan
authors: Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan.
authors: Muhammad Rashid
authors: MR
authors: Khan
authors: Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan.
authors: Sumaira
authors: S
authors: Sahreen
authors: Faculty of Biological Sciences, Department of Biotechnology, University of Science and Technology Bannu, Khyber Pakhtunkhwa, Pakistan.
authors: Mushtaq
authors: M
authors: Ahmed
authors: Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan.
authors: Naseer Ali
authors: NA
authors: Shah
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D019659:Asteraceae; D001786:Blood Glucose; D002251:Carbon Tetrachloride / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D004249:DNA Damage / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006943:Hyperglycemia / Q000139:chemically induced / Q000517:prevention & control*; D007328:Insulin / Q000097:blood; D008049:Lipase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010182:Pancreatic Diseases / Q000139:chemically induced / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D010936:Plant Extracts; D020011:Protective Agents; D002251:Carbon Tetrachloride; D008049:Lipase; D000681:Amylases
keywords: Carbon tetrachloride; DNA damages; Launaea procumbens; insulin