title: Production of high-purity neofructooligosaccharides by culture of Xanthophyllomyces dendrorhous.
abstract: Neofructooligosaccharides (neo-FOS) were produced in submerged cultures of Xanthophyllomyces dendrorhous. Among the various strains of X. dendrorhous that have intracellular (6)G-fructofuranosidase ((6)G-FFase), BCRC 21346 with high enzyme activity (3.60 U/mL) and BCRC 22367 with low enzyme activity (0.59 U/mL) were investigated in this work. Neo-FOS were generated in a 5-L jar fermenter at 20°C, 100rpm and 2vvm with the pH controlled at 6.9±0.1, using 250g/L of sucrose as the substrate. Through the catalytic action of X. dendrorhous(6)G-FFase on sucrose, monosaccharides as well as neo-FOS were produced. A portion of these monosaccharides was consumed by the yeast cells. However, the production of monosaccharides was low in concentration in culture with low (6)G-FFase activity, indicating they might be used up concurrently during the fermentation. Consequently, neo-FOS at a purity of up to 87.4% could be obtained.
authors: Department of Bioengineering, Tatung University, 104 Taipei, Taiwan. dcsheu@ttu.edu.tw
authors: Dey-Chyi
authors: DC
authors: Sheu
authors: Jan-Yi
authors: JY
authors: Chang
authors: Yan-Jen
authors: YJ
authors: Chen
authors: Cheng-Wei
authors: CW
authors: Lee
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005630:Fructans / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D009844:Oligosaccharides / Q000096:biosynthesis*; D056692:Prebiotics; D013045:Species Specificity
chemical_list: D005630:Fructans; D009844:Oligosaccharides; D056692:Prebiotics