title: HSP60 predicts survival in advanced serous ovarian cancer.
abstract: OBJECTIVE
  Heat shock protein 60 (HSP60) plays an essential role in malignant cell survival. We evaluated the prognostic and treatment predictive value of HSP60 in advanced ovarian cancer.
  
  
  METHODS
  Fresh tumor samples were prospectively collected from 123 patients undergoing primary surgery for suspected advanced ovarian cancer. Of these, 57 fulfilled the eligibility criteria, that is, International Federation of Gynecology and Obstetrics stage IIC-IV, serous/endometrioid tumors, platinum-based chemotherapy, and specimens with 50% tumor cells or greater. Heat shock protein 60 mRNA and protein expression was determined by real-time polymerase chain reaction and immunohistochemistry. We estimated the association between HSP60 and overall survival (OS) and platinum-free interval (PFI) by Cox proportional hazards models and its relationship with treatment response by Fisher's exact test. Median follow-up was 60 months.
  
  
  RESULTS
  High HSP60 mRNA expression was associated with shorter OS (hazard ratio [HR], 3.4; 95% confidence interval [CI], 1.3-8.5) and PFI (HR, 3.3; 95% CI, 1.5-7.2). Likewise, high HSP60 protein expression was associated with shorter OS (HR, 3.2; 95% CI, 1.5-7.1) and PFI (HR, 2.6; 95% CI, 1.3-5.3). Median survival for patients with high HSP60 protein expression was 31 months compared with 55 months for low expression cases (P = 0.016). The impact on OS and PFI was even stronger in the subgroup of grade 3 serous tumors. All patients with low HSP60 levels responded to first-line chemotherapy.
  
  
  CONCLUSIONS
  Heat shock protein 60 may identify groups of advanced serous ovarian cancer with different prognosis and treatment response.
authors: Department of Oncology, Karolinska University Hospital, Sweden. elisabet.hjerpe@karolinska.se
authors: Elisabet
authors: E
authors: Hjerpe
authors: Suzanne
authors: S
authors: Egyhazi
authors: Joseph
authors: J
authors: Carlson
authors: Marianne Frostvik
authors: MF
authors: Stolt
authors: Kjell
authors: K
authors: Schedvins
authors: Hemming
authors: H
authors: Johansson
authors: Maria
authors: M
authors: Shoshan
authors: Elisabeth
authors: E
authors: Avall-Lundqvist
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D005185:Fallopian Tube Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D010534:Peritoneal Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D011379:Prognosis; D011446:Prospective Studies; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D018834:Chaperonin 60; C060293:HSPD1 protein, human; D024101:Mitochondrial Proteins; D012333:RNA, Messenger