title: Rapid and accurate identification of poliovirus strains used for vaccine production.
abstract: In the context of eradication of poliomyelitis the World Health Organization stimulates the development of inactivated polio vaccines based on attenuated virus strains. In addition to vaccine development, tests have to be designed to assess the vaccine quality. An important test is the identification test for poliovirus strains that are used for the vaccine production. A rapid and accurate PCR method with fluorescent probes has been developed to identify unequivocally the vaccine-specific poliovirus strains, such as Mahoney, MEF-1, Saukett H, Sabin type 1, Sabin type 2 and Sabin type 3.
authors: National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven, The Netherlands.
authors: Olaf E M
authors: OE
authors: Nijst
authors: Justin J
authors: JJ
authors: Mouthaan
authors: Dirk R
authors: DR
authors: Mekkes
authors: Edin
authors: E
authors: Jusic
authors: Harrie G A M
authors: HG
authors: van der Avoort
authors: Bernard
authors: B
authors: Metz
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D006801:Humans; D011051:Poliomyelitis / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D017955:Poliovirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology*; D011055:Poliovirus Vaccine, Oral / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D011054:Poliovirus Vaccine, Inactivated; D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral