title: Comparison of natural and recombinant tissue factor proteins: new insights.
abstract: Tissue factor (TF), an initiator of blood coagulation in vivo, is expressed in a variety of cells. Sufficient natural TF has been isolated to clone and express recombinant proteins ranging from full-length TF to its extracellular domain. Because of the limited availability of natural TF, recombinant proteins have been used as surrogates. Despite the differences in their post-translational modifications, it has been accepted that membrane-anchored recombinant TFs are quite similar to the natural TF. Recent studies, however, have shown that post-translational modifications play an important role in TF-triggered thrombin generation.
authors: Department of Biochemistry, University of Vermont, Burlington, VT 05405, USA. sbutenas@uvm.edu
authors: Saulius
authors: S
authors: Butenas
mesh_terms: D001777:Blood Coagulation / Q000235:genetics / Q000502:physiology; D015942:Factor VIIa / Q000378:metabolism*; D006801:Humans; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D013917:Thrombin / Q000378:metabolism*; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D013925:Thromboplastin; D015942:Factor VIIa; D013917:Thrombin