title: [Effects of fatty acids on proliferation and differentiation of myoblast].
abstract: OBJECTIVE
  To explore the effects of different fatty acids on the proliferation and differentiation of myoblast.
  
  
  METHODS
  C2C12, L6, 3T3-L1 cells were treated with different concentrations (200, 100, 50, 25 and 12.5 micromol/L) of palmitic acid (PA), oleic acid (OA) or linolenic acid (LA) for different time (2, 4, 6, 8 days), the cells proliferation were tested by MTT assay. In order to observe the effects of fatty acids on differentiation of myoblasts, C2C12 and L6 cells were treated with PA, OA or LA of 25 micromol/L at the 2 days before and 0, 2, 4 days after induced differentiation, then the oil red Ostaining, Giemsa staining and creatine kinase (CK) assay were used to determine the effects of fatty acids on cell differentiation or tans-differentiation.
  
  
  RESULTS
  The inhibitory effect of the three kinds of fatty acids on cell proliferation and their time-dependent effects and dose-dependent effects were significantly different with changes of fatty acids and cell lines. PA and OA had more significant inhibition of cell proliferation than LA, and the inhibitory effects of OA showed no significant dose dependent for the three cell lines, while LA of below 100 micromol/L showed no significant time dependent for C2C12 and L6 myoblasts. Compared with 3T3-L1, cell proliferation of C2C12 and L6 was more sensitive to inhibitory effects of fatty acids. Fatty acid exposure afer differentiation had no significant effects on differention of C2C12 and L6 myoblasts, while fatty acid exposure at 2 days before differentiation remarkably affected differentiation of the myoblasts. LA exposure could increase multinucleated myotubes and creatine kinase, and promote skeletal differentiaon of myoblasts. Conversely, PA or OA exposure induced the intracellular accumulation of lipid droplets and decreased multinucleated myotubes and creatine kinase, inhibited differentiation to skeletal cells and promoted the tans-differentiation to adipocytes of myoblast.
  
  
  CONCLUSIONS
  Fatty acids exposure affect the proliferation and differentiation of in vitro cultured myoblasts, and its function was related to fatty acid composition, cell type and exposure time.
authors: School of Bioengineering of Xihua University, Key Laboratory of Food Biotechnology of Sichuan Provincial University, Chengdu 610039, China. 418284396@qq.com
authors: Guangfeng
authors: G
authors: Zhang
authors: Xianggui
authors: X
authors: Chen
authors: Lin
authors: L
authors: Lin
authors: Chunjuan
authors: C
authors: Wen
authors: Su
authors: S
authors: Rao
mesh_terms: D017667:Adipocytes / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D054338:Cell Transdifferentiation / Q000187:drug effects*; D005227:Fatty Acids / Q000494:pharmacology*; D006801:Humans; D032342:Muscle Cells / Q000166:cytology; D032446:Myoblasts / Q000166:cytology*; D019301:Oleic Acid / Q000494:pharmacology; D019308:Palmitic Acid / Q000494:pharmacology; D017962:alpha-Linolenic Acid / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D017962:alpha-Linolenic Acid; D019301:Oleic Acid; D019308:Palmitic Acid