title: A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode.
abstract: Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of "territories" of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.
authors: IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan, Italy.
authors: Hanako
authors: H
authors: Tsushima
authors: Maria Grazia
authors: MG
authors: Malabarba
authors: Stefano
authors: S
authors: Confalonieri
authors: Francesca
authors: F
authors: Senic-Matuglia
authors: Lisette G G C
authors: LG
authors: Verhoef
authors: Cristina
authors: C
authors: Bartocci
authors: Giovanni
authors: G
authors: D'Ario
authors: Andrea
authors: A
authors: Cocito
authors: Pier Paolo
authors: PP
authors: Di Fiore
authors: Anna Elisabetta
authors: AE
authors: Salcini
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry* / Q000378:metabolism*; D005786:Gene Expression Regulation; D017434:Protein Structure, Tertiary; D015203:Reproducibility of Results; D009435:Synaptic Transmission; D020798:Two-Hybrid System Techniques
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D029742:Caenorhabditis elegans Proteins; C530059:Ehs-1 protein, C elegans