title: Prospects and limits of the flow cytometric seed screen--insights from Potentilla sensu lato (Potentilleae, Rosaceae).
abstract: The flow cytometric seed screen allows for identification of reproductive modes of seed formation and inference of the ploidy of contributing gametes. However, the lack of a mathematical formalization to infer male/female genomic contributions, and the prerequisite of a binucleate female contribution to the endosperm limits its applicability. We evaluated this assumption combining a DNA-based progeny survey with a comparison of the cytology of reproductive pathways co-occurring within single individuals representing 14 Potentilleae species from six phylogenetic lineages. A numerical framework valid for sexual and pseudogamous taxa was developed, enabling quantification of female and male genomes contributing to embryo and endosperm independent of gametophyte origins, numbers of sperm involved and ploidy of parents. The inference strongly depended on accurate peak index estimation. The endosperm of Potentilleae species received a binucleate female contribution in five evolutionary lineages whereas endosperm formation remained uncertain in the Tormentillae. A modified flow cytometric seed screen protocol was developed to cope with low endosperm contents. Evolutionary conservation of a binucleate female contribution to the endosperm suggested wide applicability of flow cytometric seed screen - at least in the Potentilleae. However, alternative progeny surveys and precise embryo/endosperm ploidy estimates are required for a comprehensive understanding of the cytology of seed formation.
authors: Department of Pharmacognosy, Pharmacobotany, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria.
authors: Christoph
authors: C
authors: Dobeš
authors: Department of Pharmacognosy, Pharmacobotany, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria.
authors: Andrea
authors: A
authors: Lückl
authors: Department of Conservation Biology, Vegetation and Landscape Ecology, University of Vienna, Rennweg 14, A-1030, Vienna, Austria.|Vienna Institute for Nature Conservation & Analyses, Giessergasse 6/7, A-1090, Vienna, Austria.
authors: Karl
authors: K
authors: Hülber
authors: Department of Botany and Molecular Evolution, Senckenberg Research Institute & Biodiversity and Climate Research Centre (BiK-F), Senckenberganlage 25, D-60325, Frankfurt am Main, Germany.
authors: Juraj
authors: J
authors: Paule
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D005843:Geography; D055993:Germ Cells, Plant / Q000502:physiology; D010802:Phylogeny; D011003:Ploidies; D031987:Potentilla / Q000502:physiology*; D012098:Reproduction / Q000502:physiology; D036226:Seedlings / Q000502:physiology; D012639:Seeds / Q000502:physiology*