title: Choline-sensing carbon paste electrode containing polyaniline (pani)-silicon dioxide composite-modified choline oxidase.
abstract: In this study, a novel carbon paste electrode (CPE) was prepared using the salt form of polyaniline (pani)-silicon dioxide composite that is sensitive to choline. Choline oxidase (ChO) enzyme was immobilized to modified carbon paste electrode (MCPE) by cross-linking with glutaraldehyde. Determination of choline was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated, and the optimum parameters were found to be 6.0 and 60°C, respectively. The linear working range of the electrode was 5.0 × 10(-7)-1.0 × 10(-5) M, R(2) = 0.922. The storage stability and operation stability of the enzyme electrode were also studied.
authors: Department of Chemistry, Faculty of Sciences, Gazi University , Ankara , Türkiye.
authors: Merve
authors: M
authors: Özdemir
authors: Halit
authors: H
authors: Arslan
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D002138:Calibration; D002244:Carbon / Q000737:chemistry; D002794:Choline / Q000032:analysis*; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry; D013696:Temperature
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D018030:Silver Compounds; C416807:polyaniline; D002244:Carbon; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; C037548:silver chloride; D002794:Choline; D005976:Glutaral