title: [Genotypic and phenotypic analysis of hemolysis in foodborne Staphylococcus aureus].
abstract: OBJECTIVE
  To establish a multiplex PCR method for detecting genes of (alpha-hemolysin (hla), beta-hemolysin (hlb), hemolysin and 16S rDNA, and to learn the distribution of three hemolysin genes and the characteristics of hemolytic phenotype in 148 foodborne Staphylococcus aureus strains, and to classify the strains with cluster analysis.
  
  
  METHODS
  The multiplex PCR method was established and used to detect the genes of alpha-hemolysin, beta-hemolysin, hemolysin and 16S rDNA. The blood agar method was used to detect the characteristics of hemolytic phenotype. The experiment data was analyed with SPSS16.0.
  
  
  RESULTS
  131 strains were positive for hla gene (88.51%), 90 hlb gene (60.81%), 28 hemolysin gene (18.92%). 131 strains had the characteristics of hemolysis (88.51%), while the hemolysis were negative in 17 strains (11.49%). With the clustering factors of the hemolysin genotype and hemolytic phenotype, 148 strains were classified into 12 types from type A to type L with 100% similarity. Among them, type A contained 58 strains (39.19%), type B 37 (25.00%), type C 18 (12.16%).
  
  
  CONCLUSIONS
  This multiplex PCR method is fast, convenient and specific, and could be used for high-throughput screening of hemolysin genes in S. aureus. Most of the foodborne Staphylococcus aureus strains carrying the hla gene mainly belong to type A and type B.
authors: Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050019, China.
authors: Peinan
authors: P
authors: Wei
authors: Guoping
authors: G
authors: Lü
authors: Baohong
authors: B
authors: Xu
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D016000:Cluster Analysis; D005516:Food Microbiology; D005838:Genotype; D006460:Hemolysin Proteins / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction; D010641:Phenotype; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin; D013108:Sphingomyelin Phosphodiesterase; C029878:hlb protein, Staphylococcus aureus