title: The effect of alloferon on the enhancement of NK cell cytotoxicity against cancer via the up-regulation of perforin/granzyme B secretion.
abstract: Alloferon is a novel immunomodulatory peptide originally isolated from infected insects. It has anti-viral and anti-tumor effects via the activation of NK cells. However, specific mechanisms leading to NK cell activation and anti-tumor responses yet to be clarified. In this study, we demonstrate that alloferon increases killing activity of NK cells to cancer cells via the up-regulation of the expression of NK-activating receptors, 2B4. In addition, the production of IFN-γ and TNF-α and granule exocytosis from NK cells against cancer cell were increased by alloferon. Lastly, the anti-tumor effect of alloferon was confirmed in vivo to demonstrate effective retardation of tumor growth in the human-to-mouse xenograft model. All taken together, these results suggest that alloferon has anti-tumor effects through up-regulation of NK-activating receptor 2B4 and the enhancement of granule exocytosis from NK cells.
authors: Department of Anatomy, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Seyeon
authors: S
authors: Bae
authors: Keunhee
authors: K
authors: Oh
authors: Hyemin
authors: H
authors: Kim
authors: Yejin
authors: Y
authors: Kim
authors: Hang-Rae
authors: HR
authors: Kim
authors: Young-Il
authors: YI
authors: Hwang
authors: Dong-Sup
authors: DS
authors: Lee
authors: Jae Seung
authors: JS
authors: Kang
authors: Wang Jae
authors: WJ
authors: Lee
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology; D053804:Granzymes / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D010455:Peptides / Q000494:pharmacology*; D054353:Perforin / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000276:immunology; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D000071176:Signaling Lymphocytic Activation Molecule Family; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays
chemical_list: C510535:Alloferon; D015703:Antigens, CD; C084093:CD244 protein, human; D010455:Peptides; D011971:Receptors, Immunologic; D000071176:Signaling Lymphocytic Activation Molecule Family; D014409:Tumor Necrosis Factor-alpha; D054353:Perforin; D007371:Interferon-gamma; D053804:Granzymes