title: Interactions between subunits a and b in the rotary ATP synthase as determined by cross-linking.
abstract: The interaction of the membrane traversing stator subunits a and b of the rotary ATP synthase was probed by substitution of a single Cys into each subunit with subsequent Cu(2+) catalyzed cross-linking. Extensive interaction between the transmembrane (TM) region of one b subunit and TM2 of subunit a was indicated by cross-linking with 6 Cys pairs introduced into these regions. Additional disulfide cross-linking was observed between the N-terminus of subunit b and the periplasmic loop connecting TM4 and TM5 of subunit a. Finally, benzophenone-4-maleimide derivatized Cys in the 2-3 periplasmic loop of subunit a were shown to cross-link with the periplasmic N-terminal region of subunit b. These experiments help to define the juxtaposition of subunits b and a in the ATP synthase.
authors: Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275-0376, USA.
authors: Jessica
authors: J
authors: DeLeon-Rangel
authors: Robert R
authors: RR
authors: Ishmukhametov
authors: Warren
authors: W
authors: Jiang
authors: Robert H
authors: RH
authors: Fillingame
authors: Steven B
authors: SB
authors: Vik
mesh_terms: D025181:ATP Synthetase Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D025243:Bacterial Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001577:Benzophenones / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites / Q000235:genetics; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D008301:Maleimides / Q000737:chemistry / Q000494:pharmacology; D008958:Models, Molecular; D009154:Mutation; D011485:Protein Binding / Q000187:drug effects; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary
chemical_list: D001577:Benzophenones; D003432:Cross-Linking Reagents; D004220:Disulfides; D029968:Escherichia coli Proteins; D008301:Maleimides; C046109:benzophenone-4-maleimide; D025181:ATP Synthetase Complexes; C516212:ATP synthase b subunit, E coli; D025243:Bacterial Proton-Translocating ATPases; C074428:atpB protein, E coli; D003545:Cysteine