title: [Adaptation of methods for quantification of the effect of esterase, acetylcholinesterase and glutathione-S-transferase in Blattella germanica (Dictyoptera: Blattellidae)].
abstract: The resistance mechanisms of Blatella germanica, one of the most important urban plagues worldwide since it is a mechanical vector that houses a number of highly harmful viruses, fungi, helmints and bacteria were studied. There are different control methods used against Blattella germnanica, with insecticides playing the leading role. Their uncontrolled application has caused the development of insecticice resistance in this species. This paper adapted biochemical methods to detect the enzymes esterase, acetylcholinesterase and glutathine-S-transferase as posible resistance mechanisms. To this end, all the parameters that allow finding out if a strain is susceptible or resistant to each mechanism were set.
authors: Instituto de Medicina Tropical Pedro Kourí. cristina.diaz@infomed.sld.cu
authors: Cristina
authors: C
authors: Diaz
authors: Yudelmis
authors: Y
authors: Alvarez
authors: Yaxier
authors: Y
authors: de Armas
authors: Juan A
authors: JA
authors: Bisset
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D000818:Animals; D001681:Biological Assay; D020048:Blattellidae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002863:Chromogenic Compounds; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004950:Esterases / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D005787:Gene Frequency; D017344:Genes, Insect; D005982:Glutathione Transferase / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D008658:Inactivation, Metabolic / Q000235:genetics / Q000502:physiology*; D019476:Insect Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D007305:Insecticide Resistance / Q000235:genetics / Q000502:physiology*; D007306:Insecticides / Q000145:classification / Q000493:pharmacokinetics*; D001074:Propoxur / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013379:Substrate Specificity
chemical_list: D002800:Cholinesterase Inhibitors; D002863:Chromogenic Compounds; D019476:Insect Proteins; D007306:Insecticides; D001074:Propoxur; D005982:Glutathione Transferase; D004950:Esterases; D000110:Acetylcholinesterase