title: Effect of thiol compounds on bleomycin-induced DNA and chromosome damage in human cells.
abstract: Non-protein thiols are considered radioprotectors, preventing DNA damage by ionizing radiation. As bleomycin (BLM) is a radiomimetic agent it was proposed that thiols may prevent DNA damage produced by this antibiotic. However, results obtained with thiols and BLM-combined treatments in living cells are contradictory. The goal of this work was to assess the influence of five non-protein thiols of different electrical charge and chemical composition, on the DNA damage, DNA repair, chromosomal aberrations and cell killing induced by BLM. We found that, at the chromosomal level and cell killing, Glutathione, β-Mercaptoethanol and cysteine showed a protective effect, while ditiothreitol and cysteamine increased them, whereas at the DNA level all thiols potentiated the DNA damage induced by BLM, most probably due to a reactivation of the BLM complex.
authors: Laboratorio de Citogenética y Mutagénesis, Instituto Multidisciplinario de Biología Celular IMBICE, CCT-CONICET La Plata-Comisiôn de Investigaciones Cientîficas de la Provinincia de Buenos Aires, La Plata, Argentina.
authors: Anabela
authors: A
authors: Mira
authors: Esteban M
authors: EM
authors: Gimenez
authors: Alejandro D
authors: AD
authors: Bolzán
authors: Martha S
authors: MS
authors: Bianchi
authors: Daniel M
authors: DM
authors: López-Larraza
mesh_terms: D001761:Bleomycin / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002460:Cell Line; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D006801:Humans; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D013438:Sulfhydryl Compounds; D001761:Bleomycin