title: Quantum dots-based fluorescent probes for turn-on and turn-off sensing of butyrylcholinesterase.
abstract: A novel turn-on and turn-off sensor based on the fluorescence change of quantum dots (QDs) has been developed to detect the activity of butyrylcholinesterase (BChE). In the turn-on sensing system, we realized the detection of BChE with just one enzyme. A linear calibration plot of the activity of BChE was obtained in the wide amounts range from 10 to 1000 U/L and the detection limit was 10 U/L. In the turn-off sensing system, we realized the sensing of BChE with a wide linear relationship of 10-2000 U/L which was much wider than many other detection methods. We also studied the application in serum sample detection. The BChE sensor shows great performances. Furthermore, the turn-on and turn-off sensing mechanisms were studied extensively. These results showed that our strategy would most probably be applicable in assembling diagnostic micro-device for realizing the rapid clinical analysis of BChE.
authors: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Haidian District, Beijing, PR China.
authors: Zhenzhen
authors: Z
authors: Chen
authors: Xiangling
authors: X
authors: Ren
authors: Xianwei
authors: X
authors: Meng
authors: Longfei
authors: L
authors: Tan
authors: Dong
authors: D
authors: Chen
authors: Fangqiong
authors: F
authors: Tang
mesh_terms: D002091:Butyrylcholinesterase / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013864:Thioglycolates / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D013864:Thioglycolates; C017487:2-mercaptoacetate; D002091:Butyrylcholinesterase; C023884:thiolactic acid