title: Expression of Eag1 K+ channel and ErbBs in human pituitary adenomas: cytoskeleton arrangement patterns in cultured cells.
abstract: Pituitary adenomas can invade surrounded tissue, but the mechanism remains elusive. Ether à go-go-1 (Eag1) potassium channel and epidermal growth factor receptors (ErbB1 and ErbB2) have been associated to invasive phenotypes or poor prognosis in cancer patients. However, cells arrange their cytoskeleton in order to acquire a successful migration pattern. We have studied ErbBs and Eag1 expression, and cytoskeleton arrangements in 11 human pituitary adenomas. Eag1, ErbB1 and ErbB2 expression were studied by immunochemistry in tissue and cultured cells. The cytoskeleton arrangement was analyzed in cultured cells by immunofluorescence. Normal pituitary tissue showed ErbB2 expression and Eag1 only in few cells. However, Eag1 and ErbB2 were expressed in all the tumors analyzed. ErbB1 expression was observed variable and did not show specificity for a tumor characteristic. Cultured cells from micro- and macro-adenomas clinically functional organize their cytoskeleton suggesting a mesenchymal pattern, and a round leucocyte/amoeboid pattern from invasive clinically silent adenoma. Pituitary tumors over-express EGF receptors and the ErbB2 repeated expression suggests is a characteristic of adenomas. Eag 1 was express, in different extent, and could be a therapeutic target. The cytoskeleton arrangements observed suggest that pituitary tumor cells acquire different patterns: mesenchymal, and leucocyte/amoeboid, the last observed in the invasive adenomas. Amoeboid migration pattern has been associated with high invasion capacity.
authors: Department of Embryology, Autonomous National University of Mexico, México, México.
authors: Margarita González
authors: MG
authors: del Pliego
authors: Elsa
authors: E
authors: Aguirre-Benítez
authors: Karina
authors: K
authors: Paisano-Cerón
authors: Irene
authors: I
authors: Valdovinos-Ramírez
authors: Carlos
authors: C
authors: Rangel-Morales
authors: Verónica
authors: V
authors: Rodríguez-Mata
authors: Carmen
authors: C
authors: Solano-Agama
authors: Dolores
authors: D
authors: Martín-Tapia
authors: María Teresa
authors: MT
authors: de la Vega
authors: Miguel
authors: M
authors: Saldoval-Balanzario
authors: Javier
authors: J
authors: Camacho
authors: María Eugenia
authors: ME
authors: Mendoza-Garrido
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors / Q000378:metabolism*; D051638:Ether-A-Go-Go Potassium Channels / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D066246:ErbB Receptors; D051638:Ether-A-Go-Go Potassium Channels; D018719:Erb-b2 Receptor Tyrosine Kinases; C496884:KCNH1 protein, human
keywords: Eag1; ErbBs; Pituitary adenomas; cell culture; cytoskeleton; invasion