title: Proteomic analysis of Pseudomonas putida reveals an organic solvent tolerance-related gene mmsB.
abstract: Organic solvents are toxic to most microorganisms. However, some organic-solvent-tolerant (OST) bacteria tolerate the destructive effects of organic solvent through various accommodative mechanisms. In this work, we developed an OST adapted strain Pseudomonas putida JUCT1 that could grow in the presence of 60% (v/v) cyclohexane. Two-dimensional gel electrophoresis was used to compare and analyze the total cellular protein of P. putida JUCT1 growing with or without 60% (v/v) cyclohexane. Under different solvent conditions, five high-abundance protein spots whose intensity values show over 60% discrepancies were identified by MALDI-TOF/TOF spectra. Specifically, they are arginine deiminase, carbon-nitrogen hydrolase family putative hydrolase, 3-hydroxyisobutyrate dehydrogenase, protein chain elongation factor EF-Ts, and isochorismatase superfamily hydrolase. The corresponding genes of the latter three proteins, mmsB, tsf, and PSEEN0851, were separately expressed in Escherichia coli to evaluate their effect on OST properties of the host strain. In the presence of 4% (v/v) cyclohexane, E. coli harboring mmsB could grow to 1.70 OD(660), whereas cell growth of E. coli JM109 (the control) was completely inhibited by 2% (v/v) cyclohexane. Transformants carrying tsf or PSEEN0851 also showed an increased resistance to cyclohexane and other organic solvents compared with the control. Of these three genes, mmsB exhibited the most prominent effect on increasing OST of E. coli. Less oxidation product of cyclohexane was detected because mmsB transformants might help keep a lower intracellular cyclohexane level. This study demonstrates a feasible approach for elucidating OST mechanisms of microorganisms, and provides molecular basis to construct organic-solvent-tolerant strains for industrial applications.
authors: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, PR China. yni@jiangnan.edu.cn
authors: Ye
authors: Y
authors: Ni
authors: Liang
authors: L
authors: Song
authors: Xiaohong
authors: X
authors: Qian
authors: Zhihao
authors: Z
authors: Sun
mesh_terms: D000220:Adaptation, Biological; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003510:Cyclohexanes / Q000494:pharmacology; D004361:Drug Tolerance / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D010084:Oxidation-Reduction; D020543:Proteome; D040901:Proteomics / Q000379:methods; D016958:Pseudomonas putida / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D003510:Cyclohexanes; D020543:Proteome; D012997:Solvents; C506365:Cyclohexane