title: Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites.
abstract: Angiogenesis is essential for tissue regeneration and repair. A growing body of evidence shows that the use of bioactive glasses (BG) in biomaterial-based tissue engineering (TE) strategies may improve angiogenesis and induce increased vascularization in TE constructs. This work investigated the effect of adding nano-sized BG particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (20-30 nm) BG particles of nominally 45S5 Bioglass® composition were used to prepare composite films, which were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vivo angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen films stimulated angiogenesis by increasing by 41 % the number of blood vessels branch points. In contrast, composite films containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides the first evidence that addition of a limited concentration of n-BG (10 wt%) to collagen films induces an early angiogenic response making selected collagen/n-BG composites attractive matrices for tissue engineering and regenerative medicine.
authors: Interdisciplinary Materials Group-IESIING-UCASAL, INTECIN UBA-CONICET, A4400EDD, Salta, Argentina.
authors: Gabriela E
authors: GE
authors: Vargas
authors: Luis A
authors: LA
authors: Haro Durand
authors: Vanesa
authors: V
authors: Cadena
authors: Marcela
authors: M
authors: Romero
authors: Rosa Vera
authors: RV
authors: Mesones
authors: Mirza
authors: M
authors: Mačković
authors: Stefanie
authors: S
authors: Spallek
authors: Erdmann
authors: E
authors: Spiecker
authors: Aldo R
authors: AR
authors: Boccaccini
authors: Alejandro A
authors: AA
authors: Gorustovich
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology; D003370:Coturnix / Q000196:embryology; D004625:Embryo, Nonmammalian; D005898:Glass / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010316:Particle Size; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; C425330:bioactive glass 45S5; D003094:Collagen