title: DksA2, a zinc-independent structural analog of the transcription factor DksA.
abstract: Transcription factor DksA contains a four-Cys Zn(2 +)-finger motif thought to be responsible for structural integrity and the relative disposition of its domains. Pseudomonas aeruginosa encodes an additional DksA paralog (DksA2) that is expressed selectively under Zn(2+) limitation. Although DksA2 does not bind Zn(2+), it complements the Escherichia coli dksA deletion and has similar effects on transcription in vitro. In this study, structural and biochemical analyses reveal that DksA2 has a similar fold, domain structure and RNA polymerase binding properties to those of the E. coli DksA despite the lack of the stabilizing metal ion.
authors: Department of Microbiology, The Ohio State University, Columbus, OH, USA.
authors: Ran
authors: R
authors: Furman
authors: Tapan
authors: T
authors: Biswas
authors: Eric M
authors: EM
authors: Danhart
authors: Mark P
authors: MP
authors: Foster
authors: Oleg V
authors: OV
authors: Tsodikov
authors: Irina
authors: I
authors: Artsimovitch
mesh_terms: D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040681:Structural Homology, Protein; D014158:Transcription, Genetic; D015032:Zinc / Q000737:chemistry / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; C090751:dksA protein, E coli; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit; D015032:Zinc; D003545:Cysteine