title: Diet composition modulates expression of sirtuins and renin-angiotensin system components in adipose tissue.
abstract: OBJECTIVE
  The aim of this study was to evaluate the expression of RAS components and SIRTs enzymes in the adipose tissue of mice fed diets with different macronutrient composition.
  
  
  METHODS
  The body weight, food intake, and energy intake (kcal) were evaluated. Blood parameters (insulin sensitivity, glucose tolerance, total cholesterol, HDL-C triglyceride, and glucose levels) were also assessed. Real-time PCR was performed in epididymal adipose tissue samples to analyze the expression of renin, angiotensinogen (AGT), angiotensin-converting enzyme 1 and 2 (ACE and ACE2), and SIRTs 1-7. Male FVB/N mice were divided into 5 groups (N = 10 each) that were fed with experimental diets for 60 days. Test diets were divided into standard (ST), AIN-93M, high glucose (HG), high protein (HP) and high lipid (HL).
  
  
  RESULTS
  The main results showed that HL diet treatment induced reduction in HDL-C and triglyceride plasma levels; increased ACE (Ang II marker) expression and decreased ACE2 (Ang-[1-7] catalyzer) expression in adipose tissue; and also increased SIRT4 expression.
  
  
  CONCLUSIONS
  Diets with high lipid content induced a degenerative state associated with deregulation of adipose tissue enzymes expression.
authors: Laboratory of Health Science, Postgraduate Program in Health Sciences, University Hospital-Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Brazil.
authors: Lucinéia
authors: L
authors: de Pinho
authors: João Marcus Oliveira
authors: JM
authors: Andrade
authors: Alanna
authors: A
authors: Paraíso
authors: Aristides Batista Maia
authors: AB
authors: Filho
authors: John D
authors: JD
authors: Feltenberger
authors: André Luiz Sena
authors: AL
authors: Guimarães
authors: Alfredo Mauricio Batista
authors: AM
authors: de Paula
authors: Antônio Prates
authors: AP
authors: Caldeira
authors: Ana Cristina
authors: AC
authors: de Carvalho Botelho
authors: Maria José
authors: MJ
authors: Campagnole-Santos
authors: Sérgio Henrique
authors: SH
authors: Sousa Santos
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000808:Angiotensinogen / Q000378:metabolism; D000818:Animals; D008076:Cholesterol, HDL / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage* / Q000097:blood; D004044:Dietary Proteins / Q000008:administration & dosage; D019422:Dietary Sucrose / Q000008:administration & dosage; D005947:Glucose / Q000008:administration & dosage; D008055:Lipids / Q000097:blood*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010446:Peptide Fragments / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012083:Renin / Q000378:metabolism; D012084:Renin-Angiotensin System; D037761:Sirtuins / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D004041:Dietary Fats; D004044:Dietary Proteins; D019422:Dietary Sucrose; D008055:Lipids; D010446:Peptide Fragments; D014280:Triglycerides; D000808:Angiotensinogen; D000804:Angiotensin II; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D012083:Renin; D037761:Sirtuins; C118790:angiotensin I (1-7); D005947:Glucose