title: Cell type- and isotype-specific expression and regulation of β-tubulins in primary olfactory ensheathing cells and Schwann cells in vitro.
abstract: Olfactory ensheathing cells (OECs) and Schwann cells (SCs) are closely-related cell types with regeneration-promoting properties. Comparative gene expression analysis is particularly relevant since it may explain cell type-specific effects and guide the use of each cell type into special clinical applications. In the present study, we focused on β-tubulin isotype expression in primary adult canine glia as a translational large animal model. β-tubulins so far have been studied mainly in non-neuronal tumors and implied in tumorigenic growth. We show here that primary OECs and SCs expressed βII-V isotype mRNA. Interestingly, βIII-tubulin mRNA and protein expression was high in OECs and low in SCs, while fibroblast growth factor-2 (FGF-2) induced its down-regulation in both cell types to the same extent. This was in contrast to βV-tubulin mRNA which was similarly expressed in both cell types and unaltered by FGF-2. Immunocytochemical analysis revealed that OEC cultures contained a higher percentage of βIII-tubulin-positive cells compared to SC cultures. Addition of FGF-2 reduced the number of βIII-tubulin-positive cells in both cultures and significantly increased the percentage of cells with a multipolar morphology. Taken together, we demonstrate cell type-specific expression (βIII) and isotype-specific regulation (βIII, βV) of β-tubulin isotypes in OECs and SCs. While differential expression of βIII-tubulin in primary glial cell types with identical proliferative behaviour argues for novel functions unrelated to tumorigenic growth, strong βIII-tubulin expression in OECs may help to explain the specific properties of this glial cell type.
authors: Department of Functional and Applied Anatomy, Center of Anatomy, Hannover Medical School, Carl-Neuberg-Str.1, 30625 Hannover, Germany.
authors: Mohamed
authors: M
authors: Omar
authors: Florian
authors: F
authors: Hansmann
authors: Robert
authors: R
authors: Kreutzer
authors: Mihaela
authors: M
authors: Kreutzer
authors: Gudrun
authors: G
authors: Brandes
authors: Konstantin
authors: K
authors: Wewetzer
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D066298:In Vitro Techniques; D009833:Olfactory Pathways / Q000166:cytology*; D012583:Schwann Cells / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D014404:Tubulin; D016222:Fibroblast Growth Factor 2