title: [Visualization of the age-related changes in expressions of DNA methyltransferases in mouse oocytes using two-photon imaging system].
abstract: Quantum dot (QD) is widely used as fluorescent labeling dye for its strong anti-fluorescence quenching, high quantum yield, wide absorption spectra, and narrow emission spectra. In the present paper, QD 585-labeled DNA methyltransferases (Dnmts) and Hoechst 33342-labeled chromosomes were imaged simultaneously using two-photon imaging system. The authors' results showed that aging mouse oocytes may be not suitable for in-vitro maturation: both the localizations and expression levels of Dnmts in in-vitro matured oocytes of aging mice were changed, and these changes may be related to the abnormal DNA methylation modification.
authors: State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, China. tiann517@yahoo.cn
authors: Ning
authors: N
authors: Tian
authors: Lu
authors: L
authors: Zhang
authors: Jing-Hao
authors: JH
authors: Zheng
authors: Ying
authors: Y
authors: Li
authors: Wan-Yun
authors: WY
authors: Ma
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D005260:Female; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009865:Oocytes / Q000166:cytology / Q000201:enzymology*; D045663:Quantum Dots
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases