title: Protective effect of the roots extract of Platycodon grandiflorum on bile duct ligation-induced hepatic fibrosis in rats.
abstract: The aim of the present study was to evaluate the protective effect of aqueous extract from Platycodon grandiflorum (BC703) on bile duct ligation (BDL)-induced hepatic fibrosis in rats. BDL rats were divided into three groups, which orally received distilled water or BC703 (10 or 50 mg/kg/day) for consecutive 28 days. Antifibrotic effects of BC703 on BDL-induced hepatic fibrosis in rats were estimated by assessing serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), blood urea nitrogen (BUN), transforming growth factor-beta 1 (TGF-β1) and hepatic levels of malondialdehyde (MDA), glutathione (GSH), total superoxide dismutase (SOD) and nitric oxide (NO). The biochemical observations were supplemented by histopathological examination of liver samples stained with hematoxylin and eosin and Masson's trichrome stain. ALT, AST, TBIL and BUN were elevated in the group treated with BDL alone than in the sham-operated group. These elevations were significantly decreased by BC703 treatment. Hepatic GSH and SOD levels, depressed by BDL, were also increased in the BC703 group. In addition, increases in hepatic MDA and NO levels in the BDL-induced cholestasis were attenuated by BC703 treatment. Furthermore, BC703 treatment significantly reduced the serum level of fibrogenic cytokine, TGF-β1. Histopathological studies further substantiated the protective effect of BC703 on BDL-induced hepatic fibrosis in rat. BC703 may have beneficial effects not only on hepatic fibrosis by cholestasis but also on hepatic fibrosis development in patients with chronic hepatic disease.
authors: 1B&C Biopharm, Advanced Institutes of Convergence Technology, Suwon, Gyeonggi-Do, South Korea.
authors: J-H
authors: JH
authors: Lim
authors: T-W
authors: TW
authors: Kim
authors: I-B
authors: IB
authors: Song
authors: S-J
authors: SJ
authors: Park
authors: M-S
authors: MS
authors: Kim
authors: E-S
authors: ES
authors: Cho
authors: J-Y
authors: JY
authors: Jung
authors: H-Y
authors: HY
authors: Son
authors: J-W
authors: JW
authors: Kim
authors: H-I
authors: HI
authors: Yun
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000601:surgery; D002779:Cholestasis / Q000188:drug therapy / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008026:Ligation; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots; D031238:Platycodon; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000097:blood
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D053773:Transforming Growth Factor beta1; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Bile duct ligation; Platycodon grandiflorum; cholestasis; hepatic fibrosis