title: The role of fascin in migration and invasion of urothelial carcinoma of the bladder.
abstract: BACKGROUND
  To investigate the roles of fascin in migration and invasiveness in bladder urothelial carcinoma.
  
  
  METHODS
  Immunohistochemical detection of fascin in urothelial carcinoma samples and inhibition the expression of fascin in the urothelial carcinoma cell line were performed, then the differences in cell behaviors before and after silencing of the fascin gene were tested.
  
  
  RESULTS
  In our study, we found that overexpression of fascin was more frequent in urothelial carcinoma tissues (p < 0.001). Fascin expression was positively correlated with histological grade (p = 0.024) and pT stage (p < 0.001). After transfection of fascin shRNA, the expressions of fascin in 5637 cells and BIU87 cells were efficiently decreased according to real-time RT-PCR and Western blot analysis. When fascin was inhibited, a significant decrease in migration and invasion, and increase in adhesion were observed in 5637 cells and BIU87 cells. However, there was no significant change in the proliferation of 5637 cells or BIU87 cells with or without inhibition of the fascin gene.
  
  
  CONCLUSIONS
  Fascin expression can be used as a predictor for transformation and progression of urothelial carcinoma, and reduction of fascin levels may represent a novel therapeutic strategy for urothelial carcinoma of the bladder.
authors: Department of Urology, The First Affiliated Hospital of China Medical University, Shenyang, PR China. bijianbin@yahoo.com
authors: Jian-bin
authors: JB
authors: Bi
authors: Yuyan
authors: Y
authors: Zhu
authors: Xue-lei
authors: XL
authors: Chen
authors: Meng
authors: M
authors: Yu
authors: Yu-xi
authors: YX
authors: Zhang
authors: Bing-xun
authors: BX
authors: Li
authors: Jia-wei
authors: JW
authors: Sun
authors: Hai-lin
authors: HL
authors: Shen
authors: Chui-ze
authors: CZ
authors: Kong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D002352:Carrier Proteins / Q000502:physiology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008840:Microfilament Proteins / Q000502:physiology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D019459:Urothelium / Q000473:pathology*
chemical_list: D002352:Carrier Proteins; C502658:FSCN1 protein, human; D008840:Microfilament Proteins; D034741:RNA, Small Interfering