title: [Determination in vivo of the role of esterase and glutathione transferase enzymes in pyrethroid resistance of Aedes aegypti (Diptera: Culicidae)].
abstract: An in vivo study of two synergists, that is, Triphenil phosphate -specific esterase inhibitor- and ethacrynic acid -specific gluthation transferase inhibitor- was performed to determine if these enzymes were responsible for pyrethroid resistance of Aedes aegypti. To this end, two insecticide resistant Aedes aegypti strains were used, one strain selected with temephos by six selection generations (SAN-F6) and the other strain with delmamethrin by 12 selection generations (SAN-F12), being both strains resistant to pyrethroid insecticices. Through the use of TPP and EA synergists, it was proved that esterase and gluthation-s-transferase (GST) enzymes were responsible for pryrethroid resistance of these strains. These results showed the existence of cross-resistance and multidrug resistance, which should be taken into account for insecticide use strategies aimed at vector control.
authors: Instituto de Medicina Tropical Pedro Kouri, Ciudad de la Habana, Cuba. mrodriguez@ipk.sld.cu
authors: María Magdalena
authors: MM
authors: Rodríguez
authors: Juan A
authors: JA
authors: Bisset
authors: Ditter
authors: D
authors: Fernández
mesh_terms: D000330:Aedes / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D018432:Drug Resistance, Multiple / Q000502:physiology; D004357:Drug Synergism; D004950:Esterases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D004976:Ethacrynic Acid / Q000494:pharmacology; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D008658:Inactivation, Metabolic; D019476:Insect Proteins / Q000032:analysis / Q000502:physiology*; D007303:Insect Vectors / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D007305:Insecticide Resistance / Q000502:physiology; D007306:Insecticides / Q000493:pharmacokinetics* / Q000494:pharmacology; D007814:Larva; D007928:Lethal Dose 50; D009570:Nitriles / Q000493:pharmacokinetics; D010755:Organophosphates / Q000494:pharmacology; D011722:Pyrethrins / Q000493:pharmacokinetics*; D013045:Species Specificity; D000002:Temefos / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D010755:Organophosphates; D011722:Pyrethrins; C017160:cypermethrin; C017180:decamethrin; D005982:Glutathione Transferase; D004950:Esterases; D004976:Ethacrynic Acid; D000002:Temefos; C052570:cyfluthrin; C037304:cyhalothrin; C005445:triphenyl phosphate