title: [Diversity and enzyme-producing activity of culturable halophilic bacteria in Daishan Saltern of East China].
abstract: Soil and saline water samples were collected from the Daishan Saltern of East China, and the halophilic bacteria were isolated and cultured by using selective media, aimed to investigate the diversity and enzyme-producing activity of culturable halophilic bacteria in saltern environment. A total of 181 strains were isolated by culture-dependent method. Specific primers were used to amplify the 16S rRNA gene of bacteria and archaea. The operation taxonomy units (OTUs) were determined by ARDRA method, and the representative strain of each OTU was sequenced. The phylogenetic position of all the isolated strains was determined by 16S rRNA sequencing. The results showed that the isolated 181 strains displayed 21 operational taxonomic units (OTUs), of which, 12 OTUs belonged to halophilic bacteria, and the others belonged to halophilic archaea. Phylogenetic analysis indicated that there were 7 genera presented among the halophilic bacteria group, and 4 genera presented among the halophilic archaea group. The dominant halophilic strains were of Halomonas and Haloarcula, with 46.8% in halophilic bacteria and 49.1% in halophilic archaea group, respectively. Enzyme-producing analysis indicated that most strains displayed enzyme-producing activity, including the activities of producing amylase, proteinase and lipase, and the dominant strains capable of enzyme-producing were of Haloarcula. Our results showed that in the environment of Daishan Saltern, there existed a higher diversity of halophilic bacteria, being a source sink for screening enzyme-producing bacterial strains.
authors: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China. sdyangdandan@163.com
authors: Dan-Dan
authors: DD
authors: Yang
authors: Qian
authors: Q
authors: Li
authors: Jing-Jing
authors: JJ
authors: Huang
authors: Min
authors: M
authors: Chen
mesh_terms: D000681:Amylases / Q000378:metabolism; D044822:Biodiversity; D002681:China; D046508:Culture Techniques; D019613:Haloarcula / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D006216:Halobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D008049:Lipase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D012965:Sodium Chloride; D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases