title: Vesicle coats: structure, function, and general principles of assembly.
abstract: The transport of proteins and lipids between distinct cellular compartments is conducted by coated vesicles. These vesicles are formed by the self-assembly of coat proteins on a membrane, leading to collection of the vesicle cargo and membrane bending to form a bud. Scission at the bud neck releases the vesicle. X-ray crystallography and electron microscopy (EM) have recently generated models of isolated coat components and assembled coats. Here, we review these data to present a structural overview of the three main coats: clathrin, COPII, and COPI. The three coats have similar function, common ancestry, and structural similarities, but exhibit fundamental differences in structure and assembly. We describe the implications of structural similarities and differences for understanding the function, assembly principles, and evolution of vesicle coats.
authors: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors: Marco
authors: M
authors: Faini
authors: Rainer
authors: R
authors: Beck
authors: Felix T
authors: FT
authors: Wieland
authors: John A G
authors: JA
authors: Briggs
mesh_terms: D002966:Clathrin / Q000737:chemistry* / Q000378:metabolism; D020755:Coat Protein Complex I / Q000737:chemistry* / Q000378:metabolism; D018699:Coated Vesicles / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D020558:GTP Phosphohydrolases / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008958:Models, Molecular; D021381:Protein Transport; D033921:Vesicular Transport Proteins / Q000378:metabolism
chemical_list: D002966:Clathrin; D020755:Coat Protein Complex I; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; D020558:GTP Phosphohydrolases