title: Establishment of cellobiose utilization for lipid production in Rhodococcus opacus PD630.
abstract: Rhodococcus opacus PD630, which is known for its ability to accumulate large amounts of triacylglycerols (TAG), was metabolically engineered, and a cellobiose utilization pathway was introduced. Activities of β-glucosidases were determined, and recombinant strains accumulated fatty acids up to 39.5 ± 5.7% (wt/wt) of cell dry mass from cellobiose.
authors: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Münster, Germany.
authors: Stephan
authors: S
authors: Hetzler
authors: Alexander
authors: A
authors: Steinbüchel
mesh_terms: D002475:Cellobiose / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D060847:Metabolic Engineering; D012240:Rhodococcus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008055:Lipids; D014280:Triglycerides; D002475:Cellobiose; D005959:Glucosidases