title: An experimental porcine model of heterotopic renal autotransplantation.
abstract: OBJECTIVE
  The aim of the present study was to validate an experimental model of heterotopic renal allotransplantation. Such a model, more relevant to the human situation, has never been previously described.
  
  
  METHODS
  Pietrin pigs (40 to 50 kg) were used in the study. Through a midline incision, the left kidney was removed, washed, and preserved in a standard preservation solution (Celsior, Genzyme, France) for 20 hours at 4 °C. Heterotopic autotransplantation was performed into the right iliac fossa onto the external iliac vessels with an end-to-side anastomosis and a nonstented uretero-ureteral anastomosis was performed.
  
  
  RESULTS
  Twenty-five renal allotransplantations were performed over a 5-month time period. Mean operating time progressively decreased and stabilized after 15 procedures (mean ± SD: 78.2 ± 19 minutes and 187.4 ± 18 minutes for left nephrectomy and transplantation, respectively) as morbidity decreased concomitantly. Suturing times for end-to-side anastomosis of the renal artery and vein onto the external iliac artery and vein were 21.9 ± 7 minutes and 34 ± 8 minutes (mean ± SD), respectively. Ten pigs died before the end of the experiment.
  
  
  CONCLUSIONS
  We have developed and validated the first nonrodent animal model of heterotopic renal autotransplantation relevant to the human anatomy and physiology. The procedure was easy to learn and safe. This model could be used to teach junior surgeons renal transplantation techniques and could also be used as a model to study ischemia-reperfusion injury in renal transplantation.
authors: Department of Urology and Kidney Transplantation, Hôpital La Conception, AHPM, Aix- Marseille University, Marseille, France. alice.faure@ap-hm.fr
authors: A
authors: A
authors: Faure
authors: C
authors: C
authors: Maurin
authors: L
authors: L
authors: Bruzzese
authors: P H
authors: PH
authors: Rolland
authors: C
authors: C
authors: Coulange
authors: J
authors: J
authors: Pype
authors: V
authors: V
authors: Vidal
authors: G
authors: G
authors: Magalon
authors: E
authors: E
authors: Lechevallier
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D050377:Cold Ischemia; D007083:Iliac Artery / Q000601:surgery; D007084:Iliac Vein / Q000601:surgery; D016030:Kidney Transplantation / Q000009:adverse effects / Q000379:methods*; D023421:Models, Animal; D009392:Nephrectomy; D012077:Renal Artery / Q000601:surgery; D012082:Renal Veins / Q000601:surgery; D015203:Reproducibility of Results; D013552:Swine; D013997:Time Factors; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic; D014513:Ureter / Q000601:surgery