title: Efficient expansion of mesenchymal stromal cells in a disposable fixed bed culture system.
abstract: The need for efficient and reliable technologies for clinical-scale expansion of mesenchymal stromal cells (MSC) has led to the use of disposable bioreactors and culture systems. Here, we evaluate the expansion of cord blood-derived MSC in a disposable fixed bed culture system. Starting from an initial cell density of 6.0 × 10(7) cells, after 7 days of culture, it was possible to produce of 4.2(±0.8) × 10(8) cells, which represents a fold increase of 7.0 (±1.4). After enzymatic retrieval from Fibra-Cell disks, the cells were able to maintain their potential for differentiation into adipocytes and osteocytes and were positive for many markers common to MSC (CD73, CD90, and CD105). The results obtained in this study demonstrate that MSC can be efficiently expanded in the culture system. This novel approach presents several advantages over the current expansion systems, based on culture flasks or microcarrier-based spinner flasks and represents a key element for MSC cellular therapy according to GMP compliant clinical-scale production system.
authors: Hemotherapy Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, CEP 14051-140, Ribeirão Preto-SP, Brazil.
authors: Amanda
authors: A
authors: Mizukami
authors: Maristela D
authors: MD
authors: Orellana
authors: Sâmia R
authors: SR
authors: Caruso
authors: Karen
authors: K
authors: de Lima Prata
authors: Dimas T
authors: DT
authors: Covas
authors: Kamilla
authors: K
authors: Swiech
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D019149:Bioreactors; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D003470:Culture Media / Q000378:metabolism; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D015415:Biomarkers; D003470:Culture Media