title: Using FRET to monitor protein-induced DNA bending: the TBP-TATA complex as a model system.
abstract: Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as transcription. TATA-binding protein (TBP) is one example of a protein that elicits a conformational change in DNA; TBP binds and sharply bends its recognition sequence, which is thought to facilitate the recruitment of other protein factors. Here we describe the use of fluorescence resonance energy transfer (FRET) to evaluate DNA bending using TBP as a model system. FRET is a useful technique to measure changes in DNA conformation due to protein binding because small changes in the distance between two fluorophores (2-10 nm) translate into large changes in energy transfer.
authors: Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, USA.
authors: Rebecca H
authors: RH
authors: Blair
authors: James A
authors: JA
authors: Goodrich
authors: Jennifer F
authors: JF
authors: Kugel
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D002021:Buffers; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D016385:TATA Box; D035181:TATA-Box Binding Protein / Q000737:chemistry*
chemical_list: D002021:Buffers; D005456:Fluorescent Dyes; D035181:TATA-Box Binding Protein; C509817:TBP protein, human; D004247:DNA