title: Commensal bacteria-dependent select expression of CXCL2 contributes to periodontal tissue homeostasis.
abstract: The oral and intestinal host tissues both carry a heavy microbial burden. Although commensal bacteria contribute to healthy intestinal tissue structure and function, their contribution to oral health is poorly understood. A crucial component of periodontal health is the recruitment of neutrophils to periodontal tissue. To elucidate this process, gingival tissues of specific-pathogen-free and germ-free wild-type mice and CXCR2KO and MyD88KO mice were examined for quantitative analysis of neutrophils and CXCR2 chemoattractants (CXCL1, CXCL2). We show that the recruitment of neutrophils to the gingival tissue does not require commensal bacterial colonization but is entirely dependent on CXCR2 expression. Strikingly, however, commensal bacteria selectively upregulate the expression of CXCL2, but not CXCL1, in a MyD88-dependent way that correlates with increased neutrophil recruitment as compared with germ-free conditions. This is the first evidence that the selective use of chemokine receptor ligands contributes to neutrophil homing to healthy periodontal tissue.
authors: Department of Microbiology, University of Washington, Seattle, WA 98195, USA.
authors: Camille
authors: C
authors: Zenobia
authors: Xiao Long
authors: XL
authors: Luo
authors: Ahmed
authors: A
authors: Hashim
authors: Toshiharu
authors: T
authors: Abe
authors: Lijian
authors: L
authors: Jin
authors: Yucheng
authors: Y
authors: Chang
authors: Zhi Chao
authors: ZC
authors: Jin
authors: Jian Xun
authors: JX
authors: Sun
authors: George
authors: G
authors: Hajishengallis
authors: Mike A
authors: MA
authors: Curtis
authors: Richard P
authors: RP
authors: Darveau
mesh_terms: D000818:Animals; D001419:Bacteria / Q000472:pathogenicity*; D018407:Bacterial Physiological Phenomena; D054426:Chemokine CXCL2 / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D005881:Gingiva / Q000378:metabolism / Q000473:pathology; D006706:Homeostasis / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D053594:Myeloid Differentiation Factor 88 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000473:pathology; D010519:Periodontium / Q000378:metabolism* / Q000473:pathology; D023063:Receptors, Interleukin-8B / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D016207:Cytokines; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D023063:Receptors, Interleukin-8B