title: Cyclooxgenase-2 inhibiting perfluoropoly (ethylene glycol) ether theranostic nanoemulsions-in vitro study.
abstract: Cylcooxgenase-2 (COX-2) expressing macrophages, constituting a major portion of tumor mass, are involved in several pro-tumorigenic mechanisms. In addition, macrophages are actively recruited by the tumor and represent a viable target for anticancer therapy. COX-2 specific inhibitor, celecoxib, apart from its anticancer properties was shown to switch macrophage phenotype from tumor promoting to tumor suppressing. Celecoxib has low aqueous solubility, which may limit its tumor inhibiting effect. As opposed to oral administration, we propose that maximum anticancer effect may be achieved by nanoemulsion mediated intravenous delivery. Here we report multifunctional celecoxib nanoemulsions that can be imaged by both near-infrared fluorescence (NIRF) and (19)F magnetic resonance. Celecoxib loaded nanoemulsions showed a dose dependent uptake in mouse macrophages as measured by (19)F NMR and NIRF signal intensities of labeled cells. Dramatic inhibition of intracellular COX-2 enzyme was observed in activated macrophages upon nanoemulsion uptake. COX-2 enzyme inhibition was statistically equivalent between free drug and drug loaded nanoemulsion. However, nanoemulsion mediated drug delivery may be advantageous, helping to avoid systemic exposure to celecoxib and related side effects. Dual molecular imaging signatures of the presented nanoemulsions allow for future in vivo monitoring of the labeled macrophages and may help in examining the role of macrophage COX-2 inhibition in inflammation-cancer interactions. These features strongly support the future use of the presented nanoemulsions as anti-COX-2 theranostic nanomedicine with possible anticancer applications.
authors: Graduate School of Pharmaceutical Sciences, Mylan School of Pharmacy, Duquesne University, Pittsburgh, Pennsylvania, USA.
authors: Sravan Kumar
authors: SK
authors: Patel
authors: Yang
authors: Y
authors: Zhang
authors: John A
authors: JA
authors: Pollock
authors: Jelena M
authors: JM
authors: Janjic
mesh_terms: D000818:Animals; D000068579:Celecoxib; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D052246:Cyclooxygenase 2 Inhibitors / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D016503:Drug Delivery Systems; D004655:Emulsions; D004987:Ethers / Q000737:chemistry*; D019855:Ethylene Glycol / Q000737:chemistry*; D005466:Fluorocarbons / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D050997:Nanomedicine; D019906:Nuclear Magnetic Resonance, Biomolecular; D010316:Particle Size; D011720:Pyrazoles / Q000008:administration & dosage / Q000737:chemistry; D013449:Sulfonamides / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D004655:Emulsions; D004987:Ethers; D005466:Fluorocarbons; D011720:Pyrazoles; D013449:Sulfonamides; C078113:perfluoropolyether; D019855:Ethylene Glycol; D000068579:Celecoxib