title: Response of MC3T3-E1 osteoblasts, L929 fibroblasts, and J774 macrophages to fluoride surface-modified AZ31 magnesium alloy.
abstract: The present work evaluates the biocompatibility of a fluoride surface-modified AZ31 magnesium alloy (AZ31HF) with different cell lines that coexist in the implant environment to test its potential use as a biodegradable and absorbable biomaterial for bone repair. A clear stimulation of cell proliferation and an enhancement of the mitochondrial respiratory activity were observed when mouse osteoblasts (MC3T3-E1), fibroblasts (L929), and macrophages (J774) cell lines were cultured with the modified alloy. No significant change in apoptosis or viability rates was observed when osteoblasts and fibroblasts cultures were grown in the presence of this alloy. A proteomic analysis of the MC3T3-E1 cell extracts cultured in the presence of AZ31HF showed an overexpression of proteins related with the mineralization process, which is a necessary step for bone repair. An increase in the lactate dehydrogenase activity was observed in the MC3T3-E1 and J774 cell cultures that could be a response of the oxidative stress produced by the presence of the material. This stress could be related to the increase observed in the respiratory mitochondrial activity or respiratory burst measured in theses cultures that indicate damage in the cell membranes and subsequently some cell death. Results reported here, for and against AZ31HF, should be taken into account when considering the potential use of this modified alloy in bone repair applications.
authors: Cell-Biomaterial Recognition Laboratory, Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), c/Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors: Rosa María
authors: RM
authors: Lozano
authors: Blanca Teresa
authors: BT
authors: Pérez-Maceda
authors: Mónica
authors: M
authors: Carboneras
authors: Edgar
authors: E
authors: Onofre-Bustamante
authors: María Cristina
authors: MC
authors: García-Alonso
authors: María Lorenza
authors: ML
authors: Escudero
mesh_terms: D000497:Alloys / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D019069:Cell Respiration / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005459:Fluorides / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010455:Peptides / Q000737:chemistry; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; C586533:Mg-Al-Zn-Mn-Si-Cu alloy; D010455:Peptides; D007770:L-Lactate Dehydrogenase; D008274:Magnesium; D005459:Fluorides
keywords: AZ31 magnesium fluoride; J774 macrophages; L929 fibroblasts; MC3T3-E1 osteoblasts