title: Dung-shen (Codonopsis pilosula) attenuated the cardiac-impaired insulin-like growth factor II receptor pathway on myocardial cells.
abstract: Previous studies from our lab showed that increase in AngII in H9c2 cells causes elevated IGFII and IGFIIR through MEK and JNK, leading to rise in intracellular calcium, calcineurin activation by PLC-β3 via Gαq, insertion into mitochondrial membranes of Bad, and apoptosis via caspases 9 and 3. Codonopsis pilosula is traditionally used to lower blood pressure. The purpose of our study is to investigate if C. pilosula attenuates AngII plus Leu(27)-IGFII-induced calcium influx and apoptosis in H9c2 cardiomyoblasts. C. pilosula significantly attenuated AngII induced IGFIIR promoter activity. Leu(27)-IGFII was applied to enhance the AngII effect. C. pilosula also reversed Ca(2+) influx, MOMP and apoptosis increased by AngII plus Leu(27)-IGFII. Molecular markers in IGFIIR apoptotic pathway (IGFIIR, calcineurin, etc.) and IGFIIR-Gαq association were downregulated by C. pilosula. However, p-Bad(Ser136) and Bcl-2 were increased. Therefore, C. pilosula suppresses AngII plus Leu(27)-IGFII-induced IGFII/IGFIIR pathway in myocardial cells.
authors: Department of Emergency, China Medical University Beigang Hospital, Yunlin County, Taiwan.
authors: Kun-Hsi
authors: KH
authors: Tsai
authors: Nien-Hung
authors: NH
authors: Lee
authors: Guei-Ying
authors: GY
authors: Chen
authors: Wei-Syun
authors: WS
authors: Hu
authors: Chen-Yen
authors: CY
authors: Tsai
authors: Mu-Hsin
authors: MH
authors: Chang
authors: Gwo-Ping
authors: GP
authors: Jong
authors: Chia-Hua
authors: CH
authors: Kuo
authors: Bor-Show
authors: BS
authors: Tzang
authors: Fuu-Jen
authors: FJ
authors: Tsai
authors: Chang-Hai
authors: CH
authors: Tsai
authors: Chih-Yang
authors: CY
authors: Huang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D029745:Codonopsis / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017527:Receptor, IGF Type 2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D017527:Receptor, IGF Type 2; D002118:Calcium