title: Isolation of microorganisms able to produce 1,3-propanediol and optimization of medium constituents for Klebsiella pneumoniae AJ4.
abstract: Microbial fermentation under anaerobic and microaerobic conditions has been used for the production of 1,3-propanediol (1,3-PD), a monomer used to produce polymers such as polytrimethylene terephthalate. In this study, we screened microorganisms using the high throughput screening method and isolated the Klebsiella pneumoniae AJ4 strain, which is able to produce 1,3-PD under aerobic conditions. To obtain the maximum 1,3-PD concentration from glycerol, the response surface methodology based on a central composite design was chosen to show the statistical significance of the effects of glycerol, peptone, and (NH(4))(2)SO(4) on 1,3-PD production by K. pneumoniae AJ4. The optimal culture medium factors for achieving maximum concentrations of 1,3-PD included glycerol, 108.5 g/L; peptone, 2.72 g/L; and (NH(4))(2)SO(4), 4.38 g/L. Under this optimum condition, the maximum concentration of 1,3-PD, 54.76 g/L, was predicted. A concentration of about 52.59 g/L 1,3-PD was obtained using the optimized medium during 26-h batch fermentation, a finding that agreed well with the predicted value.
authors: Department Molecular Science and Technology, Graduate School of Interdisciplinary Program, Ajou University, Suwon, 443-749, Korea.
authors: Eunsoo
authors: E
authors: Hong
authors: Sangyoung
authors: S
authors: Yoon
authors: Jinyoung
authors: J
authors: Kim
authors: Eumin
authors: E
authors: Kim
authors: Doosub
authors: D
authors: Kim
authors: Seunggyo
authors: S
authors: Rhie
authors: Yeon-woo
authors: YW
authors: Ryu
mesh_terms: D000332:Aerobiosis / Q000187:drug effects / Q000502:physiology; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000254:growth & development* / Q000302:isolation & purification*; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol