title: Microsatellite markers for  genetic studies of the fall armyworm, Spodoptera frugiperda.
abstract: We developed six  microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera:  Noctuidae). The SSR loci were isolated with enriched genomic library protocol  by using native individuals as a genome source for markers. These loci were  characterized in 48 individuals and they were tested for the ability to  identify candidate migrants exchanged among the samples. The number of alleles  per locus ranged from 5 to 18 (10.8 on average). The observed polymorphism  information content ranged from 0.172 to 0.891. Beside the lower efficiency to  obtain SSR loci, the six microsatellites were polymorphic and sufficiently  discriminant for the genetic studies of S. frugiperda; it allowed us to  identify migrants with both NJ clustering and the Bayesian methods. These  markers will be useful for molecular ecology studies of this highly polyphagous  species in order to understand the processes that determine genetic  differentiation in the complex agro-ecosystems that it infests and improve  local integrated pest management practices.
authors: Laboratório de Resistência de Insetos a Táticas de Controle, Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors: V A C
authors: VA
authors: Pavinato
authors: S
authors: S
authors: Martinelli
authors: P F
authors: PF
authors: de Lima
authors: M I
authors: MI
authors: Zucchi
authors: C
authors: C
authors: Omoto
mesh_terms: D000483:Alleles; D000818:Animals; D001499:Bayes Theorem; D016678:Genome / Q000235:genetics; D015698:Genomic Library; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D018411:Spodoptera / Q000235:genetics*