title: Inhibitory effect of lignin during cellulose bioconversion: the effect of lignin chemistry on non-productive enzyme adsorption.
abstract: The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.
authors: VTT Technical Research Centre of Finland, VTT, Finland. jenni.rahikainen@vtt.fi
authors: Jenni L
authors: JL
authors: Rahikainen
authors: Raquel
authors: R
authors: Martin-Sampedro
authors: Harri
authors: H
authors: Heikkinen
authors: Stella
authors: S
authors: Rovio
authors: Kaisa
authors: K
authors: Marjamaa
authors: Tarja
authors: T
authors: Tamminen
authors: Orlando J
authors: OJ
authors: Rojas
authors: Kristiina
authors: K
authors: Kruus
mesh_terms: D000327:Adsorption / Q000187:drug effects; D018533:Biomass; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D017665:Hydroxyl Radical / Q000378:metabolism; D008031:Lignin / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008970:Molecular Weight; D011485:Protein Binding / Q000187:drug effects; D058525:Quartz Crystal Microbalance Techniques; D014242:Trichoderma / Q000201:enzymology
chemical_list: D017665:Hydroxyl Radical; D002482:Cellulose; D008031:Lignin; D002480:Cellulase; C109691:microcrystalline cellulose