title: Membrane interaction between Span 80 vesicle and phospholipid vesicle (liposome): Span 80 vesicle can perturb and hemifuse with liposomal membrane.
abstract: We have focused on the interaction between the Span 80 vesicle and phospholipid vesicle (liposome). The Span 80 vesicle was mixed with a phospholipid vesicle (liposome), used as a simplified model of plasma membrane. From calcein leakage experiments, it was revealed that the interaction between the Span 80 vesicles and the liposomes could perturb the liposome membrane. In the experiments based on fluorescence resonance energy transfer, the lipid mixing between Span 80 vesicle and liposome was observed. The above phenomena were also supported by the fluorescence spectroscopic analysis using laurdan, resulting in the variation in the membrane properties of liposome after its mixing with Span 80 vesicle. These results suggest that the Span 80 vesicles can easily interact and hemifuse with the liposome membrane, which depend on the membrane properties of the Span 80 vesicle, "flexible" and "molecular structure" of the components.
authors: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
authors: Keita
authors: K
authors: Hayashi
authors: Tsuyoshi
authors: T
authors: Tatsui
authors: Toshinori
authors: T
authors: Shimanouchi
authors: Hiroshi
authors: H
authors: Umakoshi
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D006601:Hexoses / Q000737:chemistry*; D008081:Liposomes; D008561:Membrane Fusion; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D006601:Hexoses; D008081:Liposomes; D010743:Phospholipids; C018665:sorbitan monooleate