title: Enzymatic removal of estrogenic activity of nonylphenol and octylphenol aqueous solutions by immobilized laccase from Trametes versicolor.
abstract: A fluidized bed reactor, filled with laccase-based beads, has been employed to bioremediate aqueous solutions polluted by endocrine disruptors belonging to the alkylphenols (APs) class. In particular Octylphenol and Nonylphenol have been studied. The catalytic activity of free and immobilized laccase from Trametes versicolor has been characterized as a function of pH, temperature and substrate concentration in the reaction medium. In view of practical applications for each substrate concentration the removal efficiency (RE), the time to halve the initial concentration (τ50), and the tc=0, i.e. the time to reach complete pollutant removal, have been calculated. The immobilized laccase exhibited a lower affinity for octylphenol (Km=1.11mM) than for Nonylphenol (Km=0.72mM), but all the other parameters of applicative interest resulted more significant for octylphenol. For example, the times to reach the complete removal of octylphenol compared to those for nonylphenol at the same concentration is shorter of about 15% (at low concentrations) up to 40% (at high concentrations). The study of cell proliferation with MPP89 cells, a human mesothelioma cell line, and the assay with the YES test indicated the loss of estrogenic activity of the APs solutions after laccase treatment.
authors: Institute of Genetics and Biophysics ABT, Via P. Castellino, 111, 80131 Naples, Italy.
authors: Maria
authors: M
authors: Catapane
authors: Carla
authors: C
authors: Nicolucci
authors: Ciro
authors: C
authors: Menale
authors: Luigi
authors: L
authors: Mita
authors: Sergio
authors: S
authors: Rossi
authors: Damiano G
authors: DG
authors: Mita
authors: Nadia
authors: N
authors: Diano
mesh_terms: D019149:Bioreactors; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D052244:Endocrine Disruptors / Q000737:chemistry; D047628:Estrogen Receptor alpha / Q000235:genetics; D004967:Estrogens / Q000737:chemistry*; D017930:Genes, Reporter; D006801:Humans; D042845:Laccase / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D020218:Response Elements; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D012996:Solutions; D055454:Trametes / Q000201:enzymology; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000494:pharmacology; D018508:Water Purification
chemical_list: C506487:ESR1 protein, human; D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D004967:Estrogens; D010636:Phenols; D012996:Solutions; D014874:Water Pollutants, Chemical; C474055:octylphenol; C025256:nonylphenol; D042845:Laccase