title: Oral glucocorticoids diminish the efficacy of allergen-specific immunotherapy in experimental feline asthma.
abstract: Allergen-specific rush immunotherapy (RIT) shows promise in treating asthma; however, pet cats will likely require at least initial concurrent glucocorticoids (GCs) to control serious clinical signs. How the immunosuppressive effects of GCs would impact RIT in cats is unknown. The hypothesis of this study was that oral, but not inhaled GCs will diminish the efficacy of RIT in experimental feline asthma. Cats (n=6/group) were sensitized using Bermuda grass allergen (BGA) and randomized to receive BGA-specific RIT for 9 months with an oral GC (prednisolone 10mg daily), inhaled GC (fluticasone 220 μg twice daily), or placebo administered for the first 6 months. Bronchoalveolar lavage fluid (BALF) percent eosinophils and other immunological assays were performed. Eosinophilic airway inflammation was suppressed in all groups at month 6 of RIT (group mean ± SD, 5 ± 2%, 13 ± 4%, and 7 ± 2% for oral GC, inhaled GC, and placebo, respectively; P=0.291). BALF percent eosinophils significantly increased over time only in oral GC/RIT cats between months 6 and 9 (P=0.031). Placebo/RIT cats had significant decreases over time in BGA-specific serum IgE (P=0.031). Concentration of interleukin (IL)-5 in BALF significantly increased over time in inhaled GC/RIT cats (P=0.031). No significant differences were found between groups at month 6 or over time in each group for BGA-specific lymphocyte blastogenesis, percent blood T regulatory cells, or number of IL-10-producing cells. Given the significant increase of airway eosinophilia over time in RIT cats initially treated with an oral GC, inhaled GCs might be better for dampening eosinophilic inflammation until RIT normalizes the dysregulated immune system.
authors: Comparative Internal Medicine Laboratory, Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA.
authors: Chee-hoon
authors: CH
authors: Chang
authors: Leah A
authors: LA
authors: Cohn
authors: Amy E
authors: AE
authors: Declue
authors: Hong
authors: H
authors: Liu
authors: Carol R
authors: CR
authors: Reinero
mesh_terms: D000284:Administration, Oral; D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000139:chemically induced* / Q000188:drug therapy; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002371:Cat Diseases / Q000139:chemically induced* / Q000188:drug therapy; D002415:Cats; D031744:Cynodon / Q000276:immunology; D003888:Desensitization, Immunologic / Q000662:veterinary*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005938:Glucocorticoids / Q000008:administration & dosage / Q000494:pharmacology*; D007073:Immunoglobulin E; D015848:Interleukin-5 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes; D008297:Male
chemical_list: D000485:Allergens; D005938:Glucocorticoids; D015848:Interleukin-5; D007073:Immunoglobulin E
keywords: Allergen-specific rush immunotherapy; Asthma; Bermuda grass allergen; Bronchoalveolar lavage fluid; Cat; Eosinophilic airway inflammation