title: eIF4GI facilitates the MicroRNA-mediated gene silencing.
abstract: MicroRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene silencing by binding to complementary target mRNAs and recruiting the miRNA-containing ribonucleoprotein complexes to the mRNAs. However, the molecular basis of this silencing is unclear. Here, we show that human Ago2 associates with the cap-binding protein complex and this association is mediated by human eIF4GI, a scaffold protein required for the translation initiation. Using a cap photo-crosslinking method, we show that Ago2 closely associates with the cap structure. Taken together, these data suggest that eIF4GI participates in the miRNA-mediated post-transcriptional gene silencing by promoting the association of Ago2 with the cap-binding complex.
authors: Molecular Virology Laboratory, POSTECH Biotech Center, Department of Life Science, Pohang University of Science and Technology, Pohang, Korea.
authors: Incheol
authors: I
authors: Ryu
authors: Ji Hoon
authors: JH
authors: Park
authors: Sihyeon
authors: S
authors: An
authors: Oh Sung
authors: OS
authors: Kwon
authors: Sung Key
authors: SK
authors: Jang
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism; D002460:Cell Line; D039603:Eukaryotic Initiation Factor-4G / Q000378:metabolism*; D005786:Gene Expression Regulation; D023061:Gene Order; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D039502:Nuclear Cap-Binding Protein Complex / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000378:metabolism; D034622:RNA Interference
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; C113920:EIF4G1 protein, human; D039603:Eukaryotic Initiation Factor-4G; D035683:MicroRNAs; D039502:Nuclear Cap-Binding Protein Complex; D012315:RNA Caps