title: [Effects of dibutyl phthalate and monobutyl phthalate on testosterone secretion and insulin-like factor 3 expression of Leydig tumor cells in mice].
abstract: OBJECTIVE
  To observe the effects of dibutyl phthalate (DBP) and monobutyl phthalate (MBP) on the mRNA and protein expression of insulin-like factor 3 (INSL3) in the Leydig tumor cells (MA-10) of mice and the level of testosterone secreted from MA-10 cells.
  
  
  METHODS
  The MA-10 cells of mice, used as a cellular model, were exposed to DBP and MBP. The content of testosterone in the supernatant medium was measured by enzyme-linked immunosorbent assay; the mRNA and protein expression levels of INSL3 in MA-10 cells were measured by quantitative PCR and Western Blot.
  
  
  RESULTS
  Compared with the control group, MA-10 cells showed increased synthesis of testosterone when exposed to low concentrations of DBP and MBP (10(-9) ∼ 10(-6) mol/L) and inhibited synthesis of testosterone when exposed to high concentrations of DBP and MBP (10(-3) mol/L), and the typical two-way effects became more significant as the time went one and the concentrations increased (P < 0.05). Compared with the control group, MA-10 cells showed significantly lower mRNA and protein expression levels of INSL3 when exposed to 10(-6) and 10(-4) mol/L DBP (P < 0.05); MA-10 cells showed increased protein expression of INSL3 when exposed to 10(-7) mol/L MBP, and the mRNA and protein expression levels of INSL3 decreased as the concentration of MBP increased.
  
  
  CONCLUSIONS
  DBP and MBP can inhibit the secretion of testosterone from MA-10 cells at high concentrations, but stimulate the secretion of testosterone at low concentrations. Both DBP and MBP have inhibitory effects on the mRNA and protein expression of INSL3 in MA-10 cells.
authors: School of Public Health, Tianjin Medical University, Tianjin 300070, China.
authors: Qing-hong
authors: QH
authors: Zhou
authors: Xi
authors: X
authors: Chen
authors: Ling
authors: L
authors: Leng
authors: Jing-shu
authors: JS
authors: Zhang
authors: Nai-jun
authors: NJ
authors: Tang
mesh_terms: D000818:Animals; D002460:Cell Line; D003993:Dibutyl Phthalate / Q000633:toxicity*; D007328:Insulin / Q000378:metabolism*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010795:Phthalic Acids / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
chemical_list: D007328:Insulin; C084797:Leydig insulin-like protein; D010795:Phthalic Acids; D011506:Proteins; D003993:Dibutyl Phthalate; D013739:Testosterone; C028577:monobutyl phthalate