title: The clearance time of infused hematopoietic stem cell from the blood circulation.
abstract: There is no detailed information about the clearance time of infused hematopoietic stem cell (HSC) from the blood circulation in humans. In this prospective study, peripheral blood CD34+ cell counts were detected during the 4days period following autologous HSC transplantation in 20 patients by means of flow cytometry. The median CD34+ cells were at the highest level in the first hour and decreased below pre-infusion values on the first day after HSC infusion. By nonparametric analysis, positive correlation was found between CD34+ cell levels at the first hour and the post-thaw CD34+ cell dose (r=0.57, p=0.01). An inverse correlation was determined between CD34+ cell levels at the first hour and neutrophil engraftment (r=-0.54, p=0.01). Compared with the patients having CD34+ cell count of ⩾2μL(-1) in the first hour following HSC infusion, the patients having CD34+ cell count of <2μL(-1) had delayed both neutrophil (20 vs. 12, p=0.008) and platelet (47 vs. 11, p=0.01) engraftments. Our results indicated that infused HSCs were removed from the blood circulation within 1day. In addition, CD34+ cell levels at the first hour may be used as an important indicator to predict the delay of neutrophil and platelet engraftments.
authors: Ege University Medical School, Department of Hematology, Izmir, Turkey. ayhan.donmez@ege.edu.tr
authors: Ayhan
authors: A
authors: Donmez
authors: Fatma
authors: F
authors: Ozsan
authors: Bahar
authors: B
authors: Arik
authors: Nese
authors: N
authors: Ozkayin
authors: Seckin
authors: S
authors: Cagirgan
authors: Sevgi
authors: S
authors: Mir
authors: Filiz
authors: F
authors: Vural
authors: Murat
authors: M
authors: Tombuloglu
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006689:Hodgkin Disease / Q000097:blood / Q000628:therapy; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000628:therapy; D036102:Peripheral Blood Stem Cell Transplantation; D010976:Platelet Count; D011446:Prospective Studies; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34