title: Dendrimers functionalized with membrane-interacting peptides for viral inhibition.
abstract: This contribution reports the synthesis of a poly(amide)-based dendrimer functionalized at the termini with a membrane-interacting peptide derived from the herpes simplex virus (HSV) type 1 glycoprotein H, namely gH625-644. This peptide has been shown to interact with model membranes and to inhibit viral infectivity. The peptidodendrimer inhibits both HSV-1 and HSV-2 at a very early stage of the entry process, most likely through an interaction with the viral envelope glycoproteins; thus, preventing the virus from coming into close contact with cellular membranes, a prerequisite of viral internalization. The 50% inhibitory concentration was 100 and 300 nM against HSV-1 and HSV-2 respectively, with no evidence of cell toxicity at these concentrations. These results show that the functionalization of a dendrimer with the peptide sequence derived from an HSV glycoprotein shows promising inhibitory activity towards viruses of the Herpesviridae family.
authors: Dipartimento di Farmacia, Università di Napoli Federico II, and DFM Scarl, Napoli, Italia.
authors: Rossella
authors: R
authors: Tarallo
authors: Tom P
authors: TP
authors: Carberry
authors: Annarita
authors: A
authors: Falanga
authors: Mariateresa
authors: M
authors: Vitiello
authors: Stefania
authors: S
authors: Galdiero
authors: Massimiliano
authors: M
authors: Galdiero
authors: Marcus
authors: M
authors: Weck
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002522:Chlorocebus aethiops; D050091:Dendrimers / Q000737:chemistry* / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000494:pharmacology; D053586:Virus Internalization / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D050091:Dendrimers; D010455:Peptides; D014759:Viral Envelope Proteins; C044200:glycoprotein H, herpes simplex virus type 1
keywords: antiviral activity; membranotropic peptides; peptidodendrimer