title: Postprandial metabolism of docosapentaenoic acid (DPA, 22:5n-3) and eicosapentaenoic acid (EPA, 20:5n-3) in humans.
abstract: The study of the metabolism of docosapentaenoic acid (DPA, 22:5n-3) in humans has been limited by the unavailability of pure DPA and the fact that DPA is found in combination with eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) in natural products. In this double blind cross over study, pure DPA and EPA were incorporated in meals served to healthy female volunteers. Mass spectrometric methods were used to study the chylomicron lipidomics. Plasma chylomicronemia was significantly reduced after the meal containing DPA compared with the meal containing EPA or olive oil only. Both EPA and DPA were incorporated into chylomicron TAGs, while there was less incorporation into chylomicron phospholipids. Lipidomic analysis of the chylomicron TAGs revealed the dynamic nature of chylomicron TAGs. The main TAG species that EPA and DPA were incorporated into were EPA/18:1/18:1, DPA/18:1/16:0 and DPA/18:1/18:1. There was very limited conversion of DPA and EPA to DHA and there were no increases in EPA levels during the 5h postprandial period after the DPA meal. In conclusion, EPA and DPA showed different metabolic fates, and DPA hindered the digestion, ingestion or incorporation into chylomicrons of the olive oil present in the meal.
authors: Department of Biochemistry and Food Chemistry, University of Turku, Turku 20014, Finland. kaisa.linderborg@utu.fi
authors: Kaisa M
authors: KM
authors: Linderborg
authors: Gunveen
authors: G
authors: Kaur
authors: Eliza
authors: E
authors: Miller
authors: Peter J
authors: PJ
authors: Meikle
authors: Amy E
authors: AE
authors: Larsen
authors: Jacquelyn M
authors: JM
authors: Weir
authors: Anu
authors: A
authors: Nuora
authors: Christopher K
authors: CK
authors: Barlow
authors: Heikki P
authors: HP
authors: Kallio
authors: David
authors: D
authors: Cameron-Smith
authors: Andrew J
authors: AJ
authors: Sinclair
mesh_terms: D000328:Adult; D002914:Chylomicrons / Q000378:metabolism; D018592:Cross-Over Studies; D004311:Double-Blind Method; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D019518:Postprandial Period; D055815:Young Adult
chemical_list: D002914:Chylomicrons; D005231:Fatty Acids, Unsaturated; D015118:Eicosapentaenoic Acid; C026219:docosapentaenoic acid