title: A standardized bamboo leaf extract inhibits monocyte adhesion to endothelial cells by modulating vascular cell adhesion protein-1.
abstract: Bamboo leaves (Phyllostachys pubescens Mazel ex J. Houz (Poacea)) have a long history of food and medical applications in Asia, including Japan and Korea. They have been used as a traditional medicine for centuries. We investigated the mechanism of anti-inflammatory activity of a bamboo leaf extract (BLE) on tumor necrosis factor-alpha (TNF-α)-induced monocyte adhesion in human umbilical vein endothelial cells (HUVECs). Exposure of HUVECs to BLE did not inhibit cell viability or cause morphological changes at concentrations ranging from 1 µg/ml to 1 mg/ml. Treatment with 0.1 mg/ml BLE caused 63% inhibition of monocyte adhesion in TNF-α-activated HUVECs, which was associated with 38.4% suppression of vascular cell adhesion molecule-1 expression. Furthermore, TNF-α-induced reactive oxygen species generation was decreased to 47.9% in BLE treated TNF-α-activated HUVECs. BLE (0.05 mg/ml) also caused about 50% inhibition of interleukin-6 secretion from lipopolysaccharide-stimulated monocyte. The results indicate that BLE may be clinically useful as an anti-inflammatory or anti-oxidant for human cardiovascular disease including atherosclerosis.
authors: Infectious Signaling Network Research Center and Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-gu, Daejeon 301-747, Korea.
authors: Sunga
authors: S
authors: Choi
authors: Myoung Soo
authors: MS
authors: Park
authors: Yu Ran
authors: YR
authors: Lee
authors: Young Chul
authors: YC
authors: Lee
authors: Tae Woo
authors: TW
authors: Kim
authors: Seon-Gil
authors: SG
authors: Do
authors: Dong Seon
authors: DS
authors: Kim
authors: Byeong Hwa
authors: BH
authors: Jeon
keywords: Bamboo leaf extracts; anti-inflammation; endothelial cells; interleukin-6; reactive oxygen species