title: On-line activity screening for radical scavengers from Baccharis chilco.
abstract: Baccharis plants have been used since ancient times in American traditional medicine. Baccharis chilco is a perennial shrub of temperate regions of South America that grows well in rainfall forests of Colombia. Neither chemical composition nor biological studies of this plant have ever been reported. Two caffeoylquinic acid (CQA) derivatives, 5-O-[(E)-caffeoyl]quinic acid (1) and 3,5-di-O-[(E)-caffeoyl]quinic acid (3), and rosmarinic acid (2) have been isolated from B. chilco growing wild in Colombia, using the on-line HPLC-DAD-DPPH radical-scavenging detection technique as guidance. In the course of the purification work, L-chiro-inositol (4) was also isolated. Structures of the four isolated compounds were determined by spectroscopic methods. Antioxidants 2 and 3 exhibited high antiradical activities evaluated by the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH(.)) assay, although somewhat lower than that of the reference compound ascorbic acid. The on-line HPLC-DAD-DPPH technique allowed a rapid pinpointing of antioxidants in the studied EtOH extract, and the facile guided isolation of the target molecules.
authors: Grupo de Investigación de Compuestos Heterocíclicos, Departamento de Química, Universidad del Valle, A.A. 25360 Cali, Valle del Cauca, Colombia.
authors: Juan C
authors: JC
authors: Argoti
authors: Pablo J
authors: PJ
authors: Linares-Palomino
authors: Sofía
authors: S
authors: Salido
authors: Bernardo
authors: B
authors: Ramírez
authors: Braulio
authors: B
authors: Insuasty
authors: Joaquín
authors: J
authors: Altarejos
mesh_terms: D031175:Baccharis / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002934:Cinnamates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D053630:Depsides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D007294:Inositol / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000097123:Rosmarinic Acid
chemical_list: D001713:Biphenyl Compounds; D002934:Cinnamates; D053630:Depsides; D016166:Free Radical Scavengers; D005609:Free Radicals; D007294:Inositol; D010851:Picrates; D010936:Plant Extracts; D011801:Quinic Acid; D000097123:Rosmarinic Acid; C472707:caffeoylquinic acid; C004931:1,1-diphenyl-2-picrylhydrazyl