title: In vitro fertilization and development of porcine oocytes matured in follicular fluid.
abstract: This study was conducted to assess the fertilization and development of porcine oocytes matured in a solo follicular fluid (pFF) using different in vitro culture systems and insemination periods. Cumulus-oocyte complexes (COCs), follicular cells (FCs), and pFF were collected from the follicles of ovaries. The pFF was used as a maturation medium (MpFF) after supplementation with follicle stimulating hormone (FSH) and antibiotics. The COCs were matured in a 15 ml test tube containing 3.5 ml of MpFF with FCs (5.2 × 10(6) cells/ml; rotating culture system) or 2 ml of MpFF without FCs in a 35-mm petri dish (static culture system) for 44 to 48 h. After maturation culture, oocytes were co-incubated with frozen-thawed spermatozoa for 5 h and then cultured for 7 days. The total mean rates of sperm penetration, normal fertilization, male pronucleus (MPN) formation, cleavage, and development to the blastocyst stage of oocytes after insemination were significantly higher (P<0.01) in the rotating culture system than in the static culture system. In conclusion, compared with the static culture system, the rotating culture system is adequate for the production of developmentally competent porcine oocytes when MpFF is used as a maturation medium.
authors: NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan.
authors: Budiyanto
authors: B
authors: Agung
authors: Takeshige
authors: T
authors: Otoi
authors: Dai-ichiro
authors: D
authors: Fuchimoto
authors: Shoichiro
authors: S
authors: Senbon
authors: Akira
authors: A
authors: Onishi
authors: Takashi
authors: T
authors: Nagai
mesh_terms: D000818:Animals; D003470:Culture Media; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D015571:Follicular Fluid; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D008297:Male; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development; D013552:Swine
chemical_list: D003470:Culture Media