title: Computational investigation of cancer-associated molecular mechanism in Aurora A (S155R) mutation.
abstract: Centrosomes are the key-regulating element of cell cycle progression. Aberrations in their functional mechanism lead to several cancer-related disorders. Aurora A protein is a centrosome-associated protein that regulates the centriole duplication and its abberations are associated with multiple cases of aneuploidy and cancer-related disorders. S155R mutation in Aurora A is reported to induce cancer like phenotype and disrupt its binding with TPX2 protein. In this study, we have demonstrated the structural consequences of Aurora A S155R mutation and the atomic changes that influenced the loss of TPX2-binding affinity. Docking and molecular dynamics simulation results suggested significant loss in atomic contacts between mutant Aurora A and TPX2 protein. Further, we observed a notable changes in conformation of mutant Aurora A-TPX2 docked complex as compared to the native. Loss of binding affinity rendered the TPX2 domain free which then induced unfolding in its coiled region and enabled the overall expansion of mutant complex as compared to the native. The significant outcomes obtained from this study will facilitate in future cancer researches and in developing the potent drug therapies.
authors: Bioinformatics Division, School of Bio Sciences and Technology, Vellore Institute of Technology University, Vellore, Tamil Nadu, India.
authors: Ambuj
authors: A
authors: Kumar
authors: Vidya
authors: V
authors: Rajendran
authors: Rao
authors: R
authors: Sethumadhavan
authors: Rituraj
authors: R
authors: Purohit
mesh_terms: D064096:Aurora Kinase A / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D009154:Mutation; D009369:Neoplasms / Q000201:enzymology* / Q000235:genetics*; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D008869:Microtubule-Associated Proteins; D064096:Aurora Kinase A