title: Cloning and Expression of Leishmania infantum LPG3 Gene by the Lizard Leishmania Expression System.
abstract: BACKGROUND
  Various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. In the present study, we used a new protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host, for the expression of LPG3 gene from Leishmania infantum (L.infantum).
  
  
  METHODS
  The LPG3 gene was cloned in the expression cassette for integration into the small subunit of the ribosomal RNA locus of Lizard Leishmania genome by electroporation. Expression of the recombinant LPG3 protein was confirmed by western blotting and immunofluorescence staining.
  
  
  RESULTS
  Western blotting confirmed the expression and production of rLPG3 protein. Immunofluoresence analysis also revealed the staining throughout the cytoplasm of transfected parasites, indicating that the protein has been expressed.
  
  
  CONCLUSIONS
  These results demonstrate that Leishmania cells can be suggested an expression system for the production of recombinant LPG3 (rLPG3) to further research in vaccine designing against leishmaniasis.
authors: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Leila
authors: L
authors: Pirdel
authors: Ahmad Zavaran
authors: AZ
authors: Hosseini
authors: Bahram
authors: B
authors: Kazemi
authors: Manoochehr
authors: M
authors: Rasouli
authors: Mojgan
authors: M
authors: Bandehpour
authors: Sara
authors: S
authors: Soudi
keywords: Leishmania; Leishmania infantum; Recombinant proteins; Vaccines