title: Identifying specific protein-DNA interactions using SILAC-based quantitative proteomics.
abstract: A comprehensive identification of protein-DNA interactions that drive processes such as transcription and replication, both in prokaryotic and eukaryotic organisms, remains a major technical challenge. In this chapter, we present a SILAC-based DNA affinity purification method that can be used to identify specific interactions between proteins and functional DNA elements in an unbiased manner.
authors: Department of Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Cornelia G
authors: CG
authors: Spruijt
authors: H Irem
authors: HI
authors: Baymaz
authors: Michiel
authors: M
authors: Vermeulen
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry; D002846:Chromatography, Affinity; D004247:DNA / Q000302:isolation & purification*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry; D011485:Protein Binding; D040901:Proteomics; D020539:Sequence Analysis, Protein
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA