title: Taenia saginata metacestode antigenic fractions obtained by ion-exchange chromatography: potential source of immunodominant markers applicable in the immunodiagnosis of human neurocysticercosis.
abstract: The aim of this study was to fractionate and partially characterize fractions obtained from the total saline extract (SE) of Taenia saginata metacestodes after ion-exchange procedure in carboxymethyl sepharose (CM) and diethylaminoethyl sepharose (DEAE) resins, as a source of antigenic markers applicable in the immunodiagnosis of neurocysticercosis (NCC). For IgG detection by enzyme-linked immunosorbent assay (ELISA) and immunoblotting, 140 serum samples were analyzed: 45 from patients with NCC (G1), 50 from patients with other parasitic infections (G2), and 45 from healthy individuals. Sensitivity (Se), specificity (Sp), area under curve (AUC), and likelihood ratios (LR) were calculated. CM S2 and DEAE S2 fractions provided high diagnostic values (Se 88.8% and 93.4%; Sp 93.7% and 92.6%; AUC 0.965 and 0.987; LR+ 14.07 and 12.67; LR- 0.11 and 0.07, respectively). In conclusion, CM S2 and DEAE S2 fractions are important sources of specific peptides, with high efficiency to diagnose NCC.
authors: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, 38400-902, Uberlândia, MG, Brazil.
authors: Daniela da Silva
authors: Dda S
authors: Nunes
authors: Henrique Tomaz
authors: HT
authors: Gonzaga
authors: Vanessa da Silva
authors: Vda S
authors: Ribeiro
authors: Jair Pereira
authors: JP
authors: da Cunha
authors: Julia Maria
authors: JM
authors: Costa-Cruz
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood*; D019540:Area Under Curve; D015415:Biomarkers; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007159:Immunologic Tests / Q000379:methods*; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000737:chemistry; D041202:Taenia saginata / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015415:Biomarkers; D012685:Sepharose