title: Cross-species amplification of selected  zebrafish, central stoneroller, and finescale dace microsatellites in lake minnow populations.
abstract: Fifteen sets of PCR primers designed for the amplification  of microsatellite loci from Danio rerio and Phoxinus neogaeus  Campostoma DNA that have been proven applicable for molecular studies of  several species of cyprinids were tested for amplification of microsatellites  from lake minnow DNA. The samples were taken from 298 fish inhabiting 6  distinct populations located in Poland, and DNA was extracted from dried fin  clips. There were 6 sets of primer loci that were identified as useful for  amplification of microsatellites from lake minnow DNA, which were polymorphic  and could be applied in population genetics of this species. Three other sets  of primers provided PCR products with a considerable number of stutter bands  obscuring the identity of true microsatellite alleles. The amplification of  lake minnow microsatellites using the 6 remaining primer sets was unsuccessful.
authors: Department of Environmental Biotechnology, University of Warmia and Mazury, Olsztyn, Poland. kaczmarczyk@uwm.edu.pl
authors: D
authors: D
authors: Kaczmarczyk
mesh_terms: D000483:Alleles; D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005828:Genetics, Population; D060106:Lakes; D018895:Microsatellite Repeats / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D011044:Poland; D013045:Species Specificity; D015027:Zebrafish / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004247:DNA