title: Highly efficient and regioselective acylation of pharmacologically interesting cordycepin catalyzed by lipase in the eco-friendly solvent 2-methyltetrahydrofuran.
abstract: A total of nine lipases and three proteases were tested for enzymatic regioselective acylation(s) of cordycepin with vinyl acetate in organic media. The highest conversion with better initial reaction rate was achieved with immobilized Candida antarctica lipase B (Novozym 435). An eco-friendly solvent 2-methyltetrahydrofuran (MeTHF) was thought to be the most suitable reaction medium. Novozym 435 was found to be a useful biocatalyst for the 25-g scale syntheses of cordycepin acetate (96.2% isolated yield), and the biocatalyst displayed excellent regioselectivity and high operational stability during the transformation. The 5'-substituted cordycepin derivative was the sole detectable product from each acylation reaction. Novozym 435 could be recycled for the synthesis of cordycepin derivative on a 25-g scale and 63% of its original activity was maintained after being reused for 7 batches. MeTHF could be considered as an eco-friendly solvent for the large scale use in biotransformation.
authors: College of Food Science & Technology, Nanjing Agricultural University, Nanjing 210095, PR China. zgchen@njau.edu.cn
authors: Zhi-Gang
authors: ZG
authors: Chen
authors: Dan-Ni
authors: DN
authors: Zhang
authors: Lin
authors: L
authors: Cao
authors: Yong-Bin
authors: YB
authors: Han
mesh_terms: D000215:Acylation; D055162:Biocatalysis; D003839:Deoxyadenosines / Q000737:chemistry* / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000378:metabolism*; D005663:Furans / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D012997:Solvents / Q000378:metabolism*; D013237:Stereoisomerism; D014753:Vinyl Compounds
chemical_list: D003839:Deoxyadenosines; D004224:Diterpenes; D005656:Fungal Proteins; D005663:Furans; D012997:Solvents; D014753:Vinyl Compounds; C030419:andrographolide; D008049:Lipase; C431349:lipase B, Candida antarctica; C550584:2-methyltetrahydrofuran; C058120:cordycepin; C011566:vinyl acetate