title: A modified yeast one-hybrid system for genome-wide identification of transcription factor binding sites.
abstract: The yeast one-hybrid system is a powerful genetic method to identify DNA-protein interactions, but there is a major limitation inherent to the system. Namely, frequency of false positives generated by yeast endogenous transcription factors has been thought to be higher than that of true positives by orders of magnitude. However, our modification efficiently can eliminate the false positives. When compared to the other methods for the analysis of DNA-protein interactions on a genome-wide scale, a modified yeast one-hybrid system offers several advantages including low initial and running cost, large-scale output, and easy handling.
authors: Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan.
authors: Kazuyuki
authors: K
authors: Yanai
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli; D015723:Gene Library; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: D014157:Transcription Factors; D004247:DNA