title: Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature.
abstract: Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.
authors: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors: Jan
authors: J
authors: Kern
authors: Roberto
authors: R
authors: Alonso-Mori
authors: Rosalie
authors: R
authors: Tran
authors: Johan
authors: J
authors: Hattne
authors: Richard J
authors: RJ
authors: Gildea
authors: Nathaniel
authors: N
authors: Echols
authors: Carina
authors: C
authors: Glöckner
authors: Julia
authors: J
authors: Hellmich
authors: Hartawan
authors: H
authors: Laksmono
authors: Raymond G
authors: RG
authors: Sierra
authors: Benedikt
authors: B
authors: Lassalle-Kaiser
authors: Sergey
authors: S
authors: Koroidov
authors: Alyssa
authors: A
authors: Lampe
authors: Guangye
authors: G
authors: Han
authors: Sheraz
authors: S
authors: Gul
authors: Dörte
authors: D
authors: Difiore
authors: Despina
authors: D
authors: Milathianaki
authors: Alan R
authors: AR
authors: Fry
authors: Alan
authors: A
authors: Miahnahri
authors: Donald W
authors: DW
authors: Schafer
authors: Marc
authors: M
authors: Messerschmidt
authors: M Marvin
authors: MM
authors: Seibert
authors: Jason E
authors: JE
authors: Koglin
authors: Dimosthenis
authors: D
authors: Sokaras
authors: Tsu-Chien
authors: TC
authors: Weng
authors: Jonas
authors: J
authors: Sellberg
authors: Matthew J
authors: MJ
authors: Latimer
authors: Ralf W
authors: RW
authors: Grosse-Kunstleve
authors: Petrus H
authors: PH
authors: Zwart
authors: William E
authors: WE
authors: White
authors: Pieter
authors: P
authors: Glatzel
authors: Paul D
authors: PD
authors: Adams
authors: Michael J
authors: MJ
authors: Bogan
authors: Garth J
authors: GJ
authors: Williams
authors: Sébastien
authors: S
authors: Boutet
authors: Johannes
authors: J
authors: Messinger
authors: Athina
authors: A
authors: Zouni
authors: Nicholas K
authors: NK
authors: Sauter
authors: Vittal K
authors: VK
authors: Yachandra
authors: Uwe
authors: U
authors: Bergmann
authors: Junko
authors: J
authors: Yano
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods; D000458:Cyanobacteria / Q000201:enzymology; D004583:Electrons; D008027:Light; D017895:Manganese Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000528:radiation effects; D011487:Protein Conformation; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D013696:Temperature; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D017895:Manganese Compounds; D010087:Oxides; D045332:Photosystem II Protein Complex; D014867:Water