title: Chitinase A from Stenotrophomonas maltophilia shows transglycosylation and antifungal activities.
abstract: Stenotrophomonas maltophilia chitinase (StmChiA and StmChiB) genes were cloned and expressed as soluble proteins of 70.5 and 41.6 kDa in Escherichia coli. Ni-NTA affinity purified StmChiA and StmChiB were optimally active at pH 5.0 and 7.0, respectively and exhibited broad range pH activity. StmChiA and StmChiB had an optimum temperature of 40°C and are stable up to 50 and 40°C, respectively. Hydrolytic activity on chitooligosaccharides indicated that StmChiA was an endo-acting enzyme releasing chitobiose and StmChiB was both exo/endo-acting enzyme with the release of GlcNAc as the final product. StmChiA showed higher preference to β-chitin and exhibited transglycosylation on even chain length tetra- and hexameric substrates. StmChiA, and not StmChiB, was active on chitinous polymers and showed antifungal activity against Fusarium oxysporum.
authors: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors: Katta
authors: K
authors: Suma
authors: Appa Rao
authors: AR
authors: Podile
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004795:Enzyme Stability / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006031:Glycosylation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein; D012995:Solubility; D020615:Stenotrophomonas maltophilia / Q000201:enzymology*; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D000935:Antifungal Agents; D002686:Chitin; D002688:Chitinases