title: AMPK phosphorylates GBF1 for mitotic Golgi disassembly.
abstract: In mammalian cells, the Golgi apparatus undergoes extensive fragmentation during mitosis; this is required not only for the partitioning of the complex but also for the process of mitosis. However, the molecular mechanism underlying the mitotic fragmentation of the Golgi is far from clear. Here, we show that AMP-activated protein kinase (AMPK) is phosphorylated and activated when cells enter mitosis. Activated AMPK phosphorylates GBF1, a guanine nucleotide exchange factor (GEF) for Arf-GTPases, disassociating GBF1 from the Golgi membrane and abolishing the action of GBF1 as an Arf1-GEF. We further demonstrate that the phosphorylation of AMPK and GBF1 is essential for Golgi disassembly and subsequent mitosis entry. These data suggest that AMPK-GBF1-Arf1 signaling is involved in the regulation of Golgi fragmentation during mitosis.
authors: Department of Biochemistry and Molecular Biology, Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
authors: Luna
authors: L
authors: Mao
authors: Ning
authors: N
authors: Li
authors: Yajuan
authors: Y
authors: Guo
authors: Xiaobin
authors: X
authors: Xu
authors: Luying
authors: L
authors: Gao
authors: Yinfeng
authors: Y
authors: Xu
authors: Linfu
authors: L
authors: Zhou
authors: Wei
authors: W
authors: Liu
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism*; D000091162:AMP-Activated Protein Kinase Kinases; D000818:Animals; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008099:Liver / Q000648:ultrastructure; D008938:Mitosis; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D015398:Signal Transduction / Q000235:genetics
chemical_list: C120445:GBF1 protein, human; D020662:Guanine Nucleotide Exchange Factors; D034741:RNA, Small Interfering; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases; D020823:ADP-Ribosylation Factor 1
keywords: AMPK; Arf1; GBF1; Golgi fragmentation; Mitosis