title: Molecular characterization of three GnRH receptor paralogs in the European eel, Anguilla anguilla: tissue-distribution and changes in transcript abundance during artificially induced sexual development.
abstract: Gonadotropin-releasing hormone receptor (GnRH-R) activation stimulates synthesis and release of gonadotropins in the vertebrate pituitary and also mediates other processes both in the brain and in peripheral tissues. To better understand the differential function of multiple GnRH-R paralogs, three GnRH-R genes (gnrhr1a, 1b, and 2) were isolated and characterized in the European eel. All three gnrhr genes were expressed in the brain and pituitary of pre-pubertal eels, and also in several peripheral tissues, notably gills and kidneys. During hormonally induced sexual maturation, pituitary expression of gnrhr1a (female) and gnrhr2 (male and female) was up-regulated in parallel with gonad development. In the brain, a clear regulation during maturation was seen only for gnrhr2 in the midbrain, with highest levels recorded during early vitellogenesis. These data suggest that GnRH-R2 is the likely hypophysiotropic GnRH-R in male eel, while both GnRH-R1a and GnRH-R2 seems to play this role in female eels.
authors: Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Valencia, Spain.
authors: David S
authors: DS
authors: Peñaranda
authors: Ilaria
authors: I
authors: Mazzeo
authors: Jon
authors: J
authors: Hildahl
authors: Victor
authors: V
authors: Gallego
authors: Rasoul
authors: R
authors: Nourizadeh-Lillabadi
authors: Luz
authors: L
authors: Pérez
authors: Juan F
authors: JF
authors: Asturiano
authors: Finn-Arne
authors: FA
authors: Weltzien
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005880:Gills / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011966:Receptors, LHRH / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D012741:Sexual Maturation / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger; D011966:Receptors, LHRH