title: Osteopontin promoter polymorphisms are associated with susceptibility to gastric cancer.
abstract: OBJECTIVE
  To evaluate the significance of osteopontin (OPN) genotypes in the susceptibility to gastric cancer.
  
  
  BACKGROUND
  The expression of OPN has been correlated with development, invasiveness, metastasis, and survival of gastric cancer, but the role of polymorphisms in the OPN promoter has not been investigated.
  
  
  METHODS
  We enrolled 146 gastric cancer patients and 128 controls. DNA was extracted from peripheral blood leucocytes. Single-nucleotide polymorphisms (SNPs) in the OPN promoter (-66, -156, -443, -616, -1748, and -1776) were analyzed by pyrosequencing and direct sequencing methods. Logistic regression analyses were used to evaluate the associations between SNPs and development of gastric cancer.
  
  
  RESULTS
  SNP -443 C/C and -616 T/T of the OPN promoter were significantly associated with gastric cancer [odds ratio (OR)=2.88; 95% confidence interval (CI), 1.16-7.12 and OR=1.95; 95% CI, 1.35-2.82, respectively]. Analysis of the combined effect of OPN promoter SNPs revealed that the combination of SNP -443 (T/C or C/C) and SNP -616 (T/T or T/G) had the most significant association with gastric cancer (OR=3.95; 95% CI, 1.58-9.90).
  
  
  CONCLUSIONS
  Our results suggest that polymorphisms in the OPN promoter are associated with the development of gastric cancer, and the combination of SNP -443 (T/C or C/C) and -616 (T/T or T/G) most significantly increases susceptibility to gastric cancer.
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.
authors: Teng-Yu
authors: TY
authors: Lee
authors: Jaw-Town
authors: JT
authors: Lin
authors: Cheng-Chung
authors: CC
authors: Wu
authors: Cheng-Chan
authors: CC
authors: Yu
authors: Ming-Shiang
authors: MS
authors: Wu
authors: Tso-Ching
authors: TC
authors: Lee
authors: Hsiao-Ping
authors: HP
authors: Chen
authors: Chun-Ying
authors: CY
authors: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011446:Prospective Studies; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D053495:Osteopontin