title: Amelioration of cognitive impairment and neurodegeneration by catechin hydrate in rat model of streptozotocin-induced experimental dementia of Alzheimer's type.
abstract: Alzheimer's disease (AD) is a progressive neurodegenerative disorder resulting in cognitive decline and enhancement of oxidative loads in the brain. Flavonoids have been considered to exert human health benefits by anti-oxidant and anti-inflammatory properties. The present study is aimed to elucidate the neuroprotective effect of catechin hydrate (CH), a natural flavanoid with potential antioxidant and anti-inflammatory properties, on intracerebroventricular streptozotocin (ICV-STZ) induced neuronal loss and memory impairment. To test this hypothesis, male Wistar rats were pretreated with CH (10 and 20mg/kgb wt) orally once daily for 21 days and then bilaterally injected with ICV-STZ (3mg/kgb wt), while sham group rats receive the same volume of vehicle. After 2 weeks of ICV-STZ infusion, rats were tested for cognitive performance using Morris water maze (MWM) test and then sacrifice for biochemical and histopathological assays. CH was found to be successful in upregulating the antioxidant status and prevented the memory loss. The expression of choline acetyl transferase (ChAT) was decreased in ICV-STZ group and CH pretreatment increases the expression of ChAT. Moreover, inflammatory mediators like TNF-α, IL-1β levels and expression of iNOS were significantly attenuated by CH pretreatment. The study suggests that CH is effective in preventing memory loss, ameliorating the oxidative stress and might be beneficial for the treatment of sporadic dementia of Alzheimer's type (SDAT).
authors: Neurotoxicology Laboratory, Department of Medical Elementology and Toxicology (Fund for the Improvement of Science and Technology Sponsored by DST and Special Assistance Programme Sponsored by UGC), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
authors: Md
authors: M
authors: Ejaz Ahmed
authors: Mohd Moshahid
authors: MM
authors: Khan
authors: Hayate
authors: H
authors: Javed
authors: Kumar
authors: K
authors: Vaibhav
authors: Andleeb
authors: A
authors: Khan
authors: Rizwana
authors: R
authors: Tabassum
authors: Mohammad
authors: M
authors: Ashafaq
authors: Farah
authors: F
authors: Islam
authors: Mohammed M
authors: MM
authors: Safhi
authors: Fakhrul
authors: F
authors: Islam
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000544:Alzheimer Disease / Q000188:drug therapy* / Q000473:pathology / Q000523:psychology; D000818:Animals; D001522:Behavior, Animal; D002392:Catechin / Q000627:therapeutic use*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003072:Cognition Disorders / Q000188:drug therapy*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D018782:Maze Learning; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D013311:Streptozocin; D002392:Catechin; D052247:Nitric Oxide Synthase Type II; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D005978:Glutathione