title: Association of T1740C polymorphism  of L-FABP with meat quality  traits in Junmu No. 1 white swine.
abstract: This  study was designed to investigate a single nucleotide polymorphism in intron 1  of the liver fatty acid-binding protein (L-FABP) gene in 156 Junmu No. 1  white swine using PCR-single-strand conformational polymorphism. The  association between the polymorphism and meat quality traits was also studied.  The cloning and sequencing results indicated that the polymorphism in intron 1  was due to a T→C mutation at position 1740 of L-FABP, yielding three  genotypes (TT, TC, and CC). Association analysis revealed that the polymorphism  had a significant effect on marbling (P < 0.05): genotype CC had more  marbling than TC, and TC had more marbling than TT. The polymorphism also had a  highly significant effect on intramuscular fat content (P < 0.01). Genotypes  CC and TC had higher intramuscular fat content than TT; there was no  significant difference between CC and TC (P > 0.05). However, no significant  conclusions concerning other traits could be drawn. We tentatively conclude  that L-FABP is a candidate gene or a quantitative trait locus-linked  gene associated with meat quality traits.
authors: Laboratory Animal Center, Jilin University, Changchun, Jilin, China.
authors: Y H
authors: YH
authors: Zhang
authors: L S
authors: LS
authors: Dai
authors: T H
authors: TH
authors: Ma
authors: S Z
authors: SZ
authors: Wang
authors: J
authors: J
authors: Guo
authors: F J
authors: FJ
authors: Li
authors: S M
authors: SM
authors: Zhang
authors: B X
authors: BX
authors: Sun
authors: D F
authors: DF
authors: Liu
authors: Y
authors: Y
authors: Gao
authors: J B
authors: JB
authors: Zhang
mesh_terms: D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D063427:Food Quality; D005838:Genotype; D007438:Introns; D008460:Meat; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA / Q000379:methods; D013552:Swine / Q000235:genetics*
chemical_list: D050556:Fatty Acid-Binding Proteins