title: Marked by association: techniques for proximity-dependent labeling of proteins in eukaryotic cells.
abstract: Various methods have been established for the purpose of identifying and characterizing protein-protein interactions (PPIs). This diverse toolbox provides researchers with options to overcome challenges specific to the nature of the proteins under investigation. Among these techniques is a category based on proximity-dependent labeling of proteins in living cells. These can be further partitioned into either hypothesis-based or unbiased screening methods, each with its own advantages and limitations. Approaches in which proteins of interest are fused to either modifying enzymes or receptor sequences allow for hypothesis-based testing of protein proximity. Protein crosslinking and BioID (proximity-dependent biotin identification) permit unbiased screening of protein proximity for a protein of interest. Here, we evaluate these approaches and their applications in living eukaryotic cells.
authors: Children's Health Research Center, Sanford Research/USD, North 60th St. East, Sioux Falls, SD, 57104, USA, kyle.roux@sanfordhealth.org.
authors: Kyle J
authors: KJ
authors: Roux
mesh_terms: D005057:Eukaryotic Cells / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D011506:Proteins