title: Production of protein-rich fungal biomass in an airlift bioreactor using vinasse as substrate.
abstract: The potential for large-scale production of an edible fungus, Rhizopus oligosporus, on a liquid residue from sugar-to-ethanol production, vinasse, was investigated. An airlift bioreactor (2.5-L working volume) was used for cultivating the fungus on 75% (v/v) vinasse with nutrient supplementation (nitrogen and phosphorus) at 37°C and pH 5.0. Aeration rates were varied from 0.5, 1.0, 1.5 to 2.0 volume(air)/volume(liquid)/min (vvm). The fungal biomass yield depended on the aeration rate, and the highest fungal biomass obtained was 8.04±0.80 (g(biomass increase)/g(initial biomass)) at 1.5vvm. The observed reductions in organic content by 80% (as soluble chemical oxygen demand) suggest the potential of recycling treated effluent as process water for in-plant use or for land applications. The fungal biomass contained ~50% crude protein and the essential amino acids contents were comparable to commercial protein sources for aquatic feeds (fishmeal and soybean meal), with the exception of methionine and phenylalanine.
authors: Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI 96822, USA.
authors: Saoharit
authors: S
authors: Nitayavardhana
authors: Kerati
authors: K
authors: Issarapayup
authors: Prasert
authors: P
authors: Pavasant
authors: Samir Kumar
authors: SK
authors: Khanal
mesh_terms: D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism*; D009930:Organic Chemicals / Q000302:isolation & purification; D010100:Oxygen / Q000378:metabolism; D012233:Rhizopus / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry; D012995:Solubility; D014866:Waste Products / Q000032:analysis*
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D009930:Organic Chemicals; D014866:Waste Products; D010100:Oxygen