title: Reduced expression of TRIF in chronic HBV infected Iranian patients.
abstract: OBJECTIVE
  TRIF is one of the main intracellular adaptor proteins required for TLR3 and 4 signaling. Abnormal gene expression of TRIF may lead to abrogated immune responses against viral infections including hepatitis B infection. The aim of this study was to identify the mRNA levels of TRIF in PBMCs isolated from chronic HBV (CHB) infected patients.
  
  
  METHODS
  mRNA was isolated from 63 CHB patients and 60 healthy controls and transcript levels of TRIF were examined in parallel with beta-actin (as housekeeping gene) using Real-Time PCR techniques.
  
  
  RESULTS
  Our results demonstrated that expression of TRIF was significantly decreased in PBMCs isolated from CHB patients when compared to healthy controls.
  
  
  CONCLUSIONS
  Based on the results reported here, it seems that CHB patients are unable to express appropriate levels of the TRIF gene, which may attenuate TLR3 and 4 signaling subsequent to HBV infection. Our results suggest a possible mechanism, which may explain why hepatitis B infection is stable in CHB patients.
authors: Physiology and Pharmacology Research center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors: Fatemeh
authors: F
authors: Ayoobi
authors: Gholamhossein
authors: G
authors: Hassanshahi
authors: Nahid
authors: N
authors: Zainodini
authors: Hossein
authors: H
authors: Khorramdelazad
authors: Mohammad Kazemi
authors: MK
authors: Arababadi
authors: Derek
authors: D
authors: Kennedy
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D016022:Case-Control Studies; D004279:DNA, Viral / Q000097:blood; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000378:metabolism*; D006801:Humans; D007492:Iran; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger; C470409:TICAM1 protein, human; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin
keywords: HBV; Hepatitis B virus; IRAK1; IRF3; Interferon regulatory transcription factor 3; Interleukin-1 receptor associated kinase-1; MYD88; NF-κB; Nuclear factor kappa-light-chain-enhancer of activated B cells; PAMP; PBMC; TIR-domain-containing adapter-inducing interferon-β; TLR; TNF receptor associated factor; TRAF6; TRIF; Toll Like Receptor; myeloid differentiation primary response; pathogen associated molecular patterns; peripheral blood mononuclear cell