title: The Arabidopsis thaliana ortholog of a purported maize cholinesterase gene encodes a GDSL-lipase.
abstract: Acetylcholinesterase is an enzyme that is intimately associated with regulation of synaptic transmission in the cholinergic nervous system and in neuromuscular junctions of animals. However the presence of cholinesterase activity has been described also in non-metazoan organisms such as slime molds, fungi and plants. More recently, a gene purportedly encoding for acetylcholinesterase was cloned from maize. We have cloned the Arabidopsis thaliana homolog of the Zea mays gene, At3g26430, and studied its biochemical properties. Our results indicate that the protein encoded by the gene exhibited lipase activity with preference to long chain substrates but did not hydrolyze choline esters. The At3g26430 protein belongs to the SGNH clan of serine hydrolases, and more specifically to the GDS(L) lipase family.
authors: School of Life Sciences and The Biodesign Institute, Arizona State University, P.O. Box 874501, Tempe, AZ 85287-4501, USA.
authors: Mrinalini
authors: M
authors: Muralidharan
authors: Kristina
authors: K
authors: Buss
authors: Katherine E
authors: KE
authors: Larrimore
authors: Nicholas A
authors: NA
authors: Segerson
authors: Latha
authors: L
authors: Kannan
authors: Tsafrir S
authors: TS
authors: Mor
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002802:Cholinesterases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods; D004789:Enzyme Activation; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006868:Hydrolysis; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D016415:Sequence Alignment; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018749:RNA, Plant; D008049:Lipase; D002802:Cholinesterases