title: Synergistic action of Galleria mellonella apolipophorin III and lysozyme against Gram-negative bacteria.
abstract: Insect immune response relies on the humoral and cellular mechanisms of innate immunity. The key factors are the antimicrobial polypeptides that act in concert against invading pathogens. Several such components, e.g. apolipophorin III (apoLp-III), lysozyme, and anionic peptide 2, are present constitutively in the hemolymph of non-challenged Galleria mellonella larvae. In the present study, we demonstrate an evidence for a synergistic action of G. mellonella lysozyme and apoLp-III against Gram-negative bacteria, providing novel insights into the mode of action of these proteins in insect antimicrobial defense. It was found that the muramidase activity of G. mellonella lysozyme considerably increased in the presence of apoLp-III. Moreover, apoLp-III enhanced the permeabilizing activity of lysozyme toward Escherichia coli cells. As shown using non-denaturing PAGE, the proteins did not form intermolecular complexes in vivo and in vitro, indicating that the effect observed was not connected with the intermolecular interactions between the proteins. Analysis of AFM images of E. coli cells exposed to G. mellonella lysozyme and/or apoLp-III revealed evident alterations in the bacterial surface structure accompanied by the changes in their biophysical properties. The bacterial cells demonstrated significant differences in elasticity, reflected by Young's modulus, as well as in adhesive forces and roughness values in comparison to the control ones. The constitutive presence of these two defense molecules in G. mellonella hemolymph and the fact that apoLp-III enhances lysozyme muramidase and perforating activities indicate that they can be regarded as important antibacterial factors acting at the early stage of infection against Gram-negative as well as Gram-positive bacteria.
authors: Department of Immunobiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors: Agnieszka
authors: A
authors: Zdybicka-Barabas
authors: Sylwia
authors: S
authors: Stączek
authors: Paweł
authors: P
authors: Mak
authors: Krzysztof
authors: K
authors: Skrzypiec
authors: Ewaryst
authors: E
authors: Mendyk
authors: Małgorzata
authors: M
authors: Cytryńska
mesh_terms: D000268:Adhesiveness; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001053:Apolipoproteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D004357:Drug Synergism; D055119:Elastic Modulus; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006458:Hemolymph / Q000201:enzymology; D019476:Insect Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000201:enzymology; D018625:Microscopy, Atomic Force; D009036:Moths / Q000196:embryology / Q000201:enzymology* / Q000276:immunology; D009113:Muramidase / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001053:Apolipoproteins; D019476:Insect Proteins; C042470:apolipophorin III; D009113:Muramidase