title: Kinetic and anion inhibition studies of a β-carbonic anhydrase (FbiCA 1) from the C4 plant Flaveria bidentis.
abstract: Several β-carbonic anhydrases (CAs, EC 4.2.1.1) are present in all land plants examined thus far. Here we report the first detailed biochemical characterization of one such isoform, FbiCA 1, from the C4 plant Flaveria bidentis, which was cloned, purified and characterized as recombinant protein. FbiCA 1 has an interesting CO2 hydrase catalytic activity (kcat of 1.2×10(5) and kcat/Km of 7.5×10(6)M(-1)×s(-1)) and was moderately inhibited by most simple/complex inorganic anions. Potent FbiCA 1 inhibitors were also detected, such as trithiocarbonate, diethyldithiocarbamate, sulfamide, sulfamic acid, phenylboronic acid and phenylarsonic acid (KIs in the range of 4-60μM). Such inhibitors may be used as tools to better understand the role of various β-CA isoforms in photosynthesis.
authors: Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Napoli, Italy. marmonti@unina.it
authors: Simona Maria
authors: SM
authors: Monti
authors: Giuseppina
authors: G
authors: De Simone
authors: Nina A
authors: NA
authors: Dathan
authors: Martha
authors: M
authors: Ludwig
authors: Daniela
authors: D
authors: Vullo
authors: Andrea
authors: A
authors: Scozzafava
authors: Clemente
authors: C
authors: Capasso
authors: Claudiu T
authors: CT
authors: Supuran
mesh_terms: D000595:Amino Acid Sequence; D000838:Anions / Q000737:chemistry* / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry* / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D037142:Flaveria / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000838:Anions; D002257:Carbonic Anhydrase Inhibitors; D020033:Protein Isoforms; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases