title: Nanoparticle-tethered NAD(+) with in situ cofactor regeneration.
abstract: A new and simple route for the preparation of immobilized NAD(+) on carboxyl-activated silica nanoparticles activated by γ-aminpropyltriethoxysilane and glutaric anhydride was developed. In addition, formate dehydrogenase, keto-reductase and the silica nanoparticle-attached NAD(+) were applied to catalyze the coupled reactions for production of L-lactate with the cofactor regenerated within the reaction cycle. As indicated by thermogravimetric analysis and FT-IR, the silica nanoparticles were successfully activated and the loading of carboxyl groups was 0.53 mmol g(-1) particle. The amount of immobilized NAD(+) on the support was 73 mg g(-1) particle. With 0.2 M pyruvate and 3 M formate, 0.16 M L-lactate was produced after the coupled reactions. The immobilized system showed excellent efficiency and stabilities in recycling, and it retained 60 % residual activity even after six reuses.
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China. liyouhui8888@126.com
authors: Youhui
authors: Y
authors: Li
authors: Hao
authors: H
authors: Liang
authors: Liwei
authors: L
authors: Sun
authors: Jianpeng
authors: J
authors: Wu
authors: Qipeng
authors: Q
authors: Yuan
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D053758:Nanoparticles; D011437:Propylamines; D019289:Pyruvic Acid / Q000378:metabolism; D012821:Silanes / Q000378:metabolism*; D012822:Silicon Dioxide
chemical_list: D003067:Coenzymes; D005561:Formates; D011437:Propylamines; D012821:Silanes; D009243:NAD; C030544:formic acid; D019344:Lactic Acid; D012822:Silicon Dioxide; D019289:Pyruvic Acid; D000429:Alcohol Oxidoreductases; D005560:Formate Dehydrogenases; C477625:amino-propyl-triethoxysilane