title: Validated HPLC-Fl method for the analysis of S-adenosylmethionine and S-adenosylhomocysteine biomarkers in human blood.
abstract: The natural methyl donor group, S-adenosylmethionine and its product, S-adenosylhomocysteine play an important role in many biochemical reactions involving transmethylation reactions. These compounds can be used as biomarkers in incipient diagnosis of various pathological disorders therefore the validation of a suitable method to routinely analysis of these compounds is very important. In this paper, a high performance liquid chromatrography method for S-adenosylmethionine and S-adenosylhomocysteine measurement as fluorescent 1,N(6)-ethanoderivatives from biological samples was validated in terms of selectivity, linearity range of the response (R > 0.9993), detection limit (9 × 10(-9) and 4.4 × 10(-9) mol L(-1)), the limit of quantitation (9.7 × 10(-9) and 5.7 × 10(-9) mol L(-1)), precision, trueness and robustness. The method for quantification simultaneous of these compounds is rapid, sensitive and precise and appropriate for clinical analysis.
authors: Centre of Bioanalysis, National Institute for Biological Sciences, Bucharest, Romania.
authors: Camelia
authors: C
authors: Albu
authors: Simona Carmen
authors: SC
authors: Litescu
authors: Gabriel Lucian
authors: GL
authors: Radu
authors: Hassan Y
authors: HY
authors: Aboul-Enein
mesh_terms: D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D016014:Linear Models; D012435:S-Adenosylhomocysteine / Q000097:blood* / Q000737:chemistry; D012436:S-Adenosylmethionine / Q000097:blood* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D015415:Biomarkers; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine