title: An insight into the proteome of the saliva of the argasid tick Ornithodoros moubata reveals important differences in saliva protein composition between the sexes.
abstract: Tick saliva contains pharmacologically active molecules that allow these parasites to obtain a blood meal from the host and facilitate host infection by tick-borne pathogens. Recent transcriptomic and proteomic analyses of the salivary glands of several tick species have provided data sets that are invaluable for a better understanding of tick sialomes and tick-host-pathogen relationships. Here we performed a proteomic study of the saliva from the argasid tick Ornithodoros moubata. Saliva samples from female and male specimens were analyzed separately by LC-MS/MS before and after their equalization to facilitate the identification of the less abundant proteins. We report the array of 193 proteins identified in the saliva of O. moubata showing: (i) the broad and complex composition of the saliva of this tick, in good agreement with the complexity of the argasid and ixodid sialomes described previously; (ii) a notable difference in the saliva proteomes of females and males, since only 10 of the proteins identified appeared to be shared by both sexes; and (iii) the presence in the salivary fluid of a wide range of proteins known to be housekeeping/intracellular, which could be secreted in unconventional ways, including exosome secretion.
authors: Parasitología, IRNASA (CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
authors: Verónica
authors: V
authors: Díaz-Martín
authors: Parasitología, IRNASA (CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
authors: Raúl
authors: R
authors: Manzano-Román
authors: Sección de Proteómica, Universidad de Valencia, Dr. Moliner, 50, 46100 Burjassot, Valencia, Spain.
authors: Luz
authors: L
authors: Valero
authors: Parasitología, IRNASA (CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
authors: Ana
authors: A
authors: Oleaga
authors: Area de Parasitología, Facultad de Farmacia, Universidad de Salamanca, 37007 Salamanca, Spain.
authors: Antonio
authors: A
authors: Encinas-Grandes
authors: Parasitología, IRNASA (CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain. Electronic address: ricardo.perez@irnasa.csic.es.
authors: Ricardo
authors: R
authors: Pérez-Sánchez
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D055354:Exosomes / Q000378:metabolism; D020224:Expressed Sequence Tags; D005260:Female; D008297:Male; D026861:Ornithodoros / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011994:Recombinant Proteins / Q000378:metabolism; D012463:Saliva / Q000378:metabolism*; D012737:Sex Factors; D053719:Tandem Mass Spectrometry
chemical_list: D000199:Actins; D020543:Proteome; D011994:Recombinant Proteins; D010751:Phosphopyruvate Hydratase