title: Biomimetic synthesis of fluorogenic quantum dots for ultrasensitive label-free detection of protease activities.
abstract: A biomimetic quantum dot synthesis-based strategy for ultrasensitive label-free detection of protease activities is reported. A dithiol peptide substrate can be activated by the protease through cleavage to form monothiol peptides, which then triggers QD growth and generates a photoluminescence signal readout. As low as 0.8 nM trypsin can be detected directly in buffer and serum and 4 pM trypsin can be detected via trypsinogen amplification with high signal to background ratios.
authors: The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou 215123, P. R. China.
authors: Xuewen
authors: X
authors: He
authors: Nan
authors: N
authors: Ma
mesh_terms: D000818:Animals; D032701:Biomimetics / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D002417:Cattle; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry; D010447:Peptide Hydrolases / Q000032:analysis*; D010455:Peptides / Q000378:metabolism; D045663:Quantum Dots / Q000737:chemistry*; D013194:Staining and Labeling; D013691:Tellurium / Q000737:chemistry; D014357:Trypsin / Q000032:analysis
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D010455:Peptides; D010447:Peptide Hydrolases; D014357:Trypsin; D013691:Tellurium; C028337:cadmium telluride
keywords: biomimetic synthesis; proteases; quantum dots; trypsin; ultrasensitive detection