title: Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling.
abstract: Cancer metastasis becomes an initial cause of cancer death in human population. In many cancers, it has been shown that the high levels of matrix metalloproteinase (MMP)-2 and/or MMP-9 are associated with the invasive phenotypes of cancer cells. In this study, we investigated the effects of cantharidin, a derivative of blister beetles which is one of the traditional Chinese medicines, on the adhesion, migration, and invasion of human bladder cancer TSGH-8301 cells. Cantharidin effectively suppressed TSGH-8301 cell adhesion, migration, and invasion in a concentration-dependent manner. Results from Western blotting, RT-PCR, and gelatin zymography assays indicated that cantharidin blocked the protein levels, gene expression (mRNA), and activities of MMP-2 and -9 in TSGH-8301 cells. Cantharidin also significantly suppressed the protein expressions of p-p38 and p-JNK1/2 in TSGH-8301 cells. Taken together, cantharidin was suggested to present antimetastatic potential via suppressing the levels of MMP-2 and MMP-9 expression that might be mediated by targeting the p38 and JNK1/2 MAPKs pathway in TSGH-8301 human bladder cancer cells.
authors: Department of Physiology, China Medical University, Taichung 404, Taiwan.
authors: Yi-Ping
authors: YP
authors: Huang
authors: Chien-Hang
authors: CH
authors: Ni
authors: Chi-Cheng
authors: CC
authors: Lu
authors: Jo-Hua
authors: JH
authors: Chiang
authors: Jai-Sing
authors: JS
authors: Yang
authors: Yang-Ching
authors: YC
authors: Ko
authors: Jing-Pin
authors: JP
authors: Lin
authors: Jehn-Hwa
authors: JH
authors: Kuo
authors: Shu-Jen
authors: SJ
authors: Chang
authors: Jing-Gung
authors: JG
authors: Chung