title: The regulation of gene expression involved in TGF-β signaling by ZNF804A, a risk gene for schizophrenia.
abstract: ZNF804A has been implicated in susceptibility to schizophrenia by several genome-wide association studies (GWAS), follow-up association studies and meta-analyses. However, the biological functions of ZNF804A are not entirely understood. To identify the genes that are affected by ZNF804A, we manipulated the expression of the ZNF804A protein in HEK293 human embryonic kidney cell lines and performed a cDNA microarray analysis followed by qPCR. We found that ZNF804A-overexpression up-regulated four genes (ANKRD1, INHBE, PIK3AP1, and DDIT3) and down-regulated three genes (CLIC2, MGAM, and BIRC3). Furthermore, we confirmed that the expression of ANKRD1, PIK3AP1, INHBE and DDIT3 at the protein level was significantly increased by ZNF804A-overexpression. This is the first report to identify genes whose protein expressions are regulated by ZNF804A. ANKRD1, PIK3AP1, INHBE and DDIT3 are related to transforming growth factor-β (TGF-β) signaling, which plays a crucial role in cell growth and differentiation. On the other hand, recent studies have reported that TGF-β signaling is associated with schizophrenia. These results provide basis for a more progressive investigation of ZNF804A contributions to the susceptibility or pathophysiology of schizophrenia.
authors: Department of Molecular Neuropsychiatry, Osaka University Graduate School of Medicine, Suita, Osaka 5650871, Japan.
authors: Satomi
authors: S
authors: Umeda-Yano
authors: Ryota
authors: R
authors: Hashimoto
authors: Hidenaga
authors: H
authors: Yamamori
authors: Takeya
authors: T
authors: Okada
authors: Yuka
authors: Y
authors: Yasuda
authors: Kazutaka
authors: K
authors: Ohi
authors: Motoyuki
authors: M
authors: Fukumoto
authors: Akira
authors: A
authors: Ito
authors: Masatoshi
authors: M
authors: Takeda
mesh_terms: D002461:Cell Line, Transformed; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D018709:Statistics, Nonparametric; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*
chemical_list: D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C540527:ZNF804A protein, human