title: Microarray analysis of the inflammatory and immune responses in head kidney turbot leucocytes treated with resveratrol.
abstract: A DNA oligo-microarray enriched in genes and involved in inflammatory and immune responses was used to evaluate the effects of resveratrol on gene expression in turbot head kidney leucocytes. Leucocytes were cultured for 3, 6 and 24 h, in the presence or absence of resveratrol, or were stimulated with the membrane fraction of the parasite Philasterides dicentrarchi or with the membrane plus resveratrol. Gene expression changed considerably in control cells, and several of the regulated genes were related to inflammatory and immune responses and to the cytoskeleton. Similar changes in gene expression occurred in control cells and in cells stimulated with P. dicentrarchi membrane fraction. Treatment with resveratrol induced changes in the expression (mostly down-regulation) of several genes involved in immune responses and inflammation. Thus, the down-regulation of the transcription factor PU.1, pentraxin-multidomain protein, heme oxygenase 1, S100 calcium-binding protein A-16 (S100A16) and the signal transducer and activator of transcription 4 was observed after all three incubation times. The down-regulation of the suppressor of cytokine signalling 3a, LPS-induced tumour necrosis alpha, hepcidin, metallothionein, TLR8 and the calcium dependent lectin A was observed after 3 and 6 h. Resveratrol also decreased the expression of CCL20, IL-8, apolipoprotein E and glutathione S-transferase after incubation for 6 and 24 h, and of TNF-α after incubation for 3 and 24 h. Resveratrol also induced strong regulation of several cytoskeleton-related genes. The use of the turbot oligo-microarray enabled us to discover genes whose expression was not previously suspected of being modulated by this polyphenol.
authors: Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors: Berta
authors: B
authors: Domínguez
authors: Belén G
authors: BG
authors: Pardo
authors: Manuel
authors: M
authors: Noia
authors: Adrián
authors: A
authors: Millán
authors: Antonio
authors: A
authors: Gómez-Tato
authors: Paulino
authors: P
authors: Martínez
authors: José
authors: J
authors: Leiro
authors: Jesús
authors: J
authors: Lamas
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002478:Cells, Cultured; D016798:Ciliophora / Q000276:immunology*; D005412:Flatfishes / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D060226:Head Kidney / Q000187:drug effects* / Q000276:immunology; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007109:Immunity / Q000187:drug effects; D007249:Inflammation / Q000235:genetics; D018836:Inflammation Mediators / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D046228:Microarray Analysis; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D000077185:Resveratrol; D009418:S100 Proteins / Q000378:metabolism; D050797:STAT4 Transcription Factor / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*; D015534:Trans-Activators / Q000378:metabolism; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D000953:Antigens, Protozoan; D051547:Heme Oxygenase-1; D018836:Inflammation Mediators; D011518:Proto-Oncogene Proteins; D000077185:Resveratrol; D009418:S100 Proteins; D050797:STAT4 Transcription Factor; D013267:Stilbenes; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1