title: Pathogenesis of type 1 (European genotype) porcine reproductive and respiratory syndrome virus in male gonads of infected boar.
abstract: The objective of this study was to determine the pathogenesis of experimental infection with a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) by defining the sites of viral replication and apoptosis in male gonads from infected boars for a period of 21 days after intranasal inoculation. Microscopically, hypospermatogenesis and abundant germ cell depletion and death were observed in the testes. Such germ cell death occurs by apoptosis, as determined by a characteristic histological patterns and evidence of massive DNA fragment detected in situ terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) reaction. PRRSV was detected in the testicular tissue of infected boars only. Viral nucleic acid was localized in spermatogonia, spermatocytes and spermatids but not in the vesicular and bulbourethral gland. In serial sections, PRRSV-positive cells did not co-localized with apoptotic cells. TUNEL-positive apoptotic cells were more numerous than PRRSV-positive cells in testicular sections. The present study demonstrated that type 1 PRRSV infects the spermatogonia and their progeny, and induces apoptosis in these germ cells.
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors: K
authors: K
authors: Han
authors: H W
authors: HW
authors: Seo
authors: Y
authors: Y
authors: Oh
authors: I
authors: I
authors: Kang
authors: C
authors: C
authors: Park
authors: J H
authors: JH
authors: Han
authors: S-H
authors: SH
authors: Kim
authors: C
authors: C
authors: Chae
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D017403:In Situ Hybridization / Q000662:veterinary; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D008297:Male; D019318:Porcine Reproductive and Respiratory Syndrome / Q000209:etiology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000502:physiology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013093:Spermatogonia / Q000166:cytology / Q000821:virology*; D018709:Statistics, Nonparametric; D013552:Swine; D013737:Testis / Q000166:cytology / Q000821:virology*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral