title: RET/PTC and PAX8/PPARγ chromosomal rearrangements in post-Chernobyl thyroid cancer and their association with iodine-131 radiation dose and other characteristics.
abstract: BACKGROUND
  Childhood exposure to iodine-131 from the 1986 nuclear accident in Chernobyl, Ukraine, led to a sharp increase in papillary thyroid carcinoma (PTC) incidence in regions surrounding the reactor. Data concerning the association between genetic mutations in PTCs and individual radiation doses are limited.
  
  
  METHODS
  Mutational analysis was performed on 62 PTCs diagnosed in a Ukrainian cohort of patients who were < 18 years old in 1986 and received 0.008 to 8.6 Gy of (131) I to the thyroid. Associations between mutation types and (131) I dose and other characteristics were explored.
  
  
  RESULTS
  RET/PTC (ret proto-oncogene/papillary thyroid carcinoma) rearrangements were most common (35%), followed by BRAF (15%) and RAS (8%) point mutations. Two tumors carrying PAX8/PPARγ (paired box 8/peroxisome proliferator-activated receptor gamma) rearrangement were identified. A significant negative association with (131) I dose for BRAF and RAS point mutations and a significant concave association with (131) I dose, with an inflection point at 1.6 Gy and odds ratio of 2.1, based on a linear-quadratic model for RET/PTC and PAX8/PPARγ rearrangements were found. The trends with dose were significantly different between tumors with point mutations and rearrangements. Compared with point mutations, rearrangements were associated with residence in the relatively iodine-deficient Zhytomyr region, younger age at exposure or surgery, and male sex.
  
  
  CONCLUSIONS
  These results provide the first demonstration of PAX8/PPARγ rearrangements in post-Chernobyl tumors and show different associations for point mutations and chromosomal rearrangements with (131) I dose and other factors. These data support the relationship between chromosomal rearrangements, but not point mutations, and (131) I exposure and point to a possible role of iodine deficiency in generation of RET/PTC rearrangements in these patients.
authors: Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
authors: Rebecca J
authors: RJ
authors: Leeman-Neill
authors: Alina V
authors: AV
authors: Brenner
authors: Mark P
authors: MP
authors: Little
authors: Tetiana I
authors: TI
authors: Bogdanova
authors: Maureen
authors: M
authors: Hatch
authors: Liudmyla Y
authors: LY
authors: Zurnadzy
authors: Kiyohiko
authors: K
authors: Mabuchi
authors: Mykola D
authors: MD
authors: Tronko
authors: Yuri E
authors: YE
authors: Nikiforov
mesh_terms: D000293:Adolescent; D000328:Adult; D002277:Carcinoma / Q000209:etiology* / Q000235:genetics / Q000601:surgery; D002291:Carcinoma, Papillary; D047852:Chernobyl Nuclear Accident; D002648:Child; D004252:DNA Mutational Analysis; D003677:Deficiency Diseases / Q000150:complications; D005260:Female; D015321:Gene Rearrangement / Q000528:radiation effects*; D006801:Humans; D007455:Iodine / Q000172:deficiency*; D007457:Iodine Radioisotopes / Q000633:toxicity*; D008297:Male; D015999:Multivariate Analysis; D016017:Odds Ratio; D000072636:PAX8 Transcription Factor; D047495:PPAR gamma / Q000235:genetics*; D051761:Paired Box Transcription Factors / Q000235:genetics*; D017354:Point Mutation; D000090063:Proto-Oncogene Mas; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics*; D011832:Radiation Injuries / Q000150:complications* / Q000209:etiology; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000209:etiology* / Q000235:genetics / Q000601:surgery; D013997:Time Factors; D014455:Ukraine / Q000453:epidemiology
chemical_list: D007457:Iodine Radioisotopes; C000715470:MAS1 protein, human; D000072636:PAX8 Transcription Factor; C083325:PAX8 protein, human; D047495:PPAR gamma; D051761:Paired Box Transcription Factors; D000090063:Proto-Oncogene Mas; D007455:Iodine; D051096:Proto-Oncogene Proteins c-ret; C099282:RET protein, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf