title: SMC1A knockdown induces growth suppression of human lung adenocarcinoma cells through G1/S cell cycle phase arrest and apoptosis pathways in vitro.
abstract: SMC1A (structural maintenance of chromosomes 1A), which encodes a structural subunit of the cohesin protein complex, is necessary for the process of sister chromatid cohesion during the cell cycle. Mutation and deregulation of SMC1A are highly relevant to diverse human diseases, including Cornelia de Lange syndrome and malignant carcinomas. In order to further investigate the role of SMC1A in the oncogenesis of lung cancer, SMC1A-specific short hairpin RNA (shRNA)-expressing lentivirus (Lv-shSMC1A) was constructed and used to infect A549 and H1299 cells. SMC1A mRNA and protein expression levels were downregulated in A549 and H1299 cells as demonstrated by real-time PCR and western blot assays. We found that SMC1A inhibition resulted in significantly impaired proliferation and colony formation as well as reduced invasiveness of tumor cells. Notably, Lv-shSMC1A-infected cancer cells exhibited a greater proportion of cells in the G0/G1 phase, but a lower proportion of S phase cells, compared to the parent or Lv-shCon infected cancer cells. Moreover, a greater proportion of sub-G1 apoptotic cells was observed in Lv-shSMC1A-infected cells. These results suggest that SMC1A is a novel proliferation regulator that promotes the growth of lung cancer cells, and that down-regulation of SMC1A expression induces growth suppression of A549 and H1299 cells via G1/S cell cycle phase arrest and apoptosis pathways. Therefore, SMC1A may serve as a new molecular target for lung cancer therapy.
authors: Department of Thoracic Surgery, The Second Hospital of Jilin University, Changchun 130041;
authors: Yi-Fan
authors: YF
authors: Zhang
authors: Rui
authors: R
authors: Jiang
authors: Jin-Dong
authors: JD
authors: Li
authors: Xing-Yi
authors: XY
authors: Zhang
authors: Peng
authors: P
authors: Zhao
authors: Miao
authors: M
authors: He
authors: Hou-Zhong
authors: HZ
authors: Zhang
authors: Li-Ping
authors: LP
authors: Sun
authors: Dong-Lei
authors: DL
authors: Shi
authors: Guang-Xin
authors: GX
authors: Zhang
authors: Mei
authors: M
authors: Sun
keywords: SMC1A; lung cancer; proliferation; shRNA