title: Metabolism of profenofos to 4-bromo-2-chlorophenol, a specific and sensitive exposure biomarker.
abstract: Profenofos is a direct acting phosphorothioate organophosphorus (OP) pesticide capable of inhibiting β-esterases such as acetylcholinesterase, butyrylcholinesterase, and carboxylesterase. Profenofos is known to be detoxified to the biologically inactive metabolite, 4-bromo-2-chlorophenol (BCP); however, limited data are available regarding the use of urinary BCP as an exposure biomarker in humans. A pilot study conducted in Egyptian agriculture workers, demonstrated that urinary BCP levels prior to application (3.3-30.0 μg/g creatinine) were elevated to 34.5-3,566 μg/g creatinine during the time workers were applying profenofos to cotton fields. Subsequently, the in vitro enzymatic formation of BCP was examined using pooled human liver microsomes and recombinant human cytochrome P-450s (CYPs) incubated with profenofos. Of the nine human CYPs studied, only CYPs 3A4, 2B6, and 2C19 were able to metabolize profenofos to BCP. Kinetic studies indicated that CYP 2C19 has the lowest Km, 0.516 μM followed by 2B6 (Km=1.02 μM) and 3A4 (Km=18.9μM). The Vmax for BCP formation was 47.9, 25.1, and 19.2 nmol/min/nmol CYP for CYP2B6, 2C19, and 3A4, respectively. Intrinsic clearance (Vmax/Km) values of 48.8, 46.9, and 1.02 ml/min/nmol CYP 2C19, 2B6, and 3A4, respectively, indicate that CYP2C19 and CYP2B6 are primarily responsible for the detoxification of profenofos. These findings support the use of urinary BCP as a biomarker of exposure to profenofos in humans and suggest polymorphisms in CYP 2C19 and CYP 2B6 as potential biomarkers of susceptibility.
authors: Department of Pharmacology & Toxicology, State University of New York at Buffalo, Buffalo, NY, USA.
authors: Oswald A
authors: OA
authors: Dadson
authors: Corie A
authors: CA
authors: Ellison
authors: Steven T
authors: ST
authors: Singleton
authors: Lai-Har
authors: LH
authors: Chi
authors: Barbara P
authors: BP
authors: McGarrigle
authors: Pamela J
authors: PJ
authors: Lein
authors: Fayssal M
authors: FM
authors: Farahat
authors: Taghreed
authors: T
authors: Farahat
authors: James R
authors: JR
authors: Olson
mesh_terms: D000383:Agriculture; D015415:Biomarkers / Q000652:urine; D002733:Chlorophenols / Q000493:pharmacokinetics* / Q000652:urine; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004534:Egypt; D006801:Humans; D008658:Inactivation, Metabolic; D007306:Insecticides / Q000493:pharmacokinetics*; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D016273:Occupational Exposure / Q000032:analysis; D063086:Organothiophosphates / Q000493:pharmacokinetics*; D010865:Pilot Projects
chemical_list: D015415:Biomarkers; D002733:Chlorophenols; D007306:Insecticides; D063086:Organothiophosphates; C024273:profenofos; D003577:Cytochrome P-450 Enzyme System; C075205:4-bromo-2-chlorophenol