title: Investigation of the cumulative body burden of estrogen-3,4-quinone in breast cancer patients and controls using albumin adducts as biomarkers.
abstract: Both 17β-estradiol-2,3-quinone (E2-2,3-Q) and 17β-estradiol-3,4-quinone (E2-3,4-Q) are reactive metabolites of estrogen. Elevation of E2-3,4-Q to E2-2,3-Q ratio is thought to be an important indicator of estrogen-induced carcinogenesis. Our current study compared the cumulative body burden of these estrogen quinones in serum samples taken from Taiwanese women with breast cancer (n=152) vs healthy controls (n=75) by using albumin (Alb) adducts as biomarkers. Results clearly demonstrated the presence of cysteinyl adducts of E2-2,3-Q-4-S-Alb and E2-3,4-Q-2-S-Alb in all study population at levels ranging from 61.7-1330 to 66.6-1,590 pmol/g, respectively. Correlation coefficient between E2-2,3-Q-4-S-Alb and E2-3,4-Q-2-S-Alb was 0.610 for controls and 0.767 for breast cancer patients (p<0.001). We also noticed that in premenopausal subjects with body mass index (BMI) less than 27, background levels of E2-3,4-Q-2-S-Alb was inversely proportional to BMI with about 25% increase in E2-3,4-Q-2-S-Alb per 5 kg/m(2) decrease in BMI (p<0.001). In addition, we confirmed that mean levels of E2-3,4-Q-2-S-Alb in breast cancer patients were ∼5-fold greater than in those of controls (p<0.001). Overall, this evidence suggests that disparity in estrogen disposition and the subsequent elevation of cumulative body burden of E2-3,4-Q may play a role in the development of breast cancer.
authors: Comprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua, Taiwan.
authors: Che
authors: C
authors: Lin
authors: Dar-Ren
authors: DR
authors: Chen
authors: Wei-Chung
authors: WC
authors: Hsieh
authors: Wen-Fa
authors: WF
authors: Yu
authors: Ching-Chiuan
authors: CC
authors: Lin
authors: Mao-Huei
authors: MH
authors: Ko
authors: Chang-Hsin
authors: CH
authors: Juan
authors: Ben-Jei
authors: BJ
authors: Tsuang
authors: Po-Hsiung
authors: PH
authors: Lin
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001822:Body Burden; D015992:Body Mass Index; D001943:Breast Neoplasms / Q000097:blood*; D016022:Case-Control Studies; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006801:Humans; D016014:Linear Models; D008875:Middle Aged; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D000075462:Serum Albumin, Human; D013624:Taiwan; D015854:Up-Regulation
chemical_list: C422455:ALB protein, human; D015415:Biomarkers; D012709:Serum Albumin; C500988:estradiol-3,4-quinone; C049872:estradiol-2,3-O-quinone; D004958:Estradiol; D000075462:Serum Albumin, Human