title: PTS1 peroxisomal import pathway plays shared and distinct roles to PTS2 pathway in development and pathogenicity of Magnaporthe oryzae.
abstract: Peroxisomes participate in various important metabolisms and are required in pathogenicity of fungal plant pathogens. Peroxisomal matrix proteins are imported from cytoplasm into peroxisomes through peroxisomal targeting signal 1 (PTS1) or peroxisomal targeting signal 2 (PTS2) import pathway. PEX5 and PEX7 genes participate in the two pathways respectively. The involvement of PEX7 mediated PTS2 import pathway in fungal pathogenicity has been documented, while that of PTS1 remains unclear. Through null mutant analysis of MoPEX5, the PEX5 homolog in Magnaporthe oryzae, we report the crucial roles of PTS1 pathway in the development and host infection in the rice blast fungus, and compared with those of PTS2. We found that MoPEX5 disruption specifically blocked the PTS1 pathway. Δmopex5 was unable to use lipids as sole carbon source and lost pathogenicity completely. Similar as Δmopex7, Δmopex5 exhibited significant reduction in lipid utilization and mobilization, appressorial turgor genesis and H(2)O(2) resistance. Additionally, Δmopex5 presented some distinct defects which were undetected in Δmopex7 in vegetative growth, conidial morphogenesis, appressorial morphogenesis and melanization. The results indicated that the PTS1 peroxisomal import pathway, in addition to PTS2, is required for fungal development and pathogenicity of the rice blast fungus, and also, as a main peroxisomal import pathway, played a more predominant role than PTS2.
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors: Jiaoyu
authors: J
authors: Wang
authors: Zhen
authors: Z
authors: Zhang
authors: Yanli
authors: Y
authors: Wang
authors: Ling
authors: L
authors: Li
authors: Rongyao
authors: R
authors: Chai
authors: Xueqin
authors: X
authors: Mao
authors: Hua
authors: H
authors: Jiang
authors: Haiping
authors: H
authors: Qiu
authors: Xinfa
authors: X
authors: Du
authors: Fucheng
authors: F
authors: Lin
authors: Guochang
authors: G
authors: Sun
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D020082:Magnaporthe / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000382:microbiology*; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000378:metabolism*; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology*; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013172:Spores, Fungal / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear; D006861:Hydrogen Peroxide