title: A minimally invasive and simple screening test for detection of pancreatic ductal adenocarcinoma using biomarkers in duodenal juice.
abstract: OBJECTIVE
  The aim of this study was to establish a minimally invasive and simple screening test for detection of pancreatic ductal adenocarcinoma (PDAC) using duodenal juice (DJ).
  
  
  METHODS
  Duodenal juice was collected prospectively before endoscopic retrograde cholangiopancreatography in 46 patients. A protease inhibitor was not added to the samples collected during the initial 2.5 minutes but was added in the latter 2.5 minutes. Thereafter, secretin was administered intravenously, and DJ was subsequently collected for additional 10 minutes. The sensitivities of carcinoembryonic antigen (CEA), S100 calcium-binding protein P (S100P), and interleukin 8 in DJ and pancreatic juice were assessed.
  
  
  RESULTS
  There were 30 patients with PDAC and 16 with benign lesions. It was possible to collect an adequate amount of DJ without secretin administration. In the PDAC group, CEA concentrations in DJ were significantly higher than those in the benign group, even without the use of a protease inhibitor. S100P levels in DJ in the PDAC group were significantly higher than those in the benign group in the presence of the protease inhibitor.
  
  
  CONCLUSIONS
  Duodenal juice collection during routine upper endoscopy and assessments of CEA and S100P in DJ might become a useful screening test for detection of PDAC.
authors: Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors: Yasuhisa
authors: Y
authors: Mori
authors: Takao
authors: T
authors: Ohtsuka
authors: Hiroshi
authors: H
authors: Kono
authors: Yosuke
authors: Y
authors: Nagayoshi
authors: Noboru
authors: N
authors: Ideno
authors: Teppei
authors: T
authors: Aso
authors: Shingo
authors: S
authors: Kozono
authors: Kenoki
authors: K
authors: Ohuchida
authors: Shunichi
authors: S
authors: Takahata
authors: Masafumi
authors: M
authors: Nakamura
authors: Kazuhiro
authors: K
authors: Mizumoto
authors: Masao
authors: M
authors: Tanaka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism* / Q000473:pathology; D002760:Cholangiopancreatography, Endoscopic Retrograde; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007419:Intestinal Secretions / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D011480:Protease Inhibitors / Q000008:administration & dosage; D012372:ROC Curve; D012633:Secretin / Q000008:administration & dosage; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; C508606:CXCL8 protein, human; D002135:Calcium-Binding Proteins; D002272:Carcinoembryonic Antigen; D016209:Interleukin-8; D009363:Neoplasm Proteins; D011480:Protease Inhibitors; C103003:S100P protein, human; D012633:Secretin