title: Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice.
abstract: This study aimed to demonstrate protective activities of the narirutin fraction from peels of Citrus unshiu against ethanol-induced hepatic damage through an animal study. Citrus narirutin fraction (CNF), contained 75% of narirutin, was obtained by an ultra-sonicated extraction and further purification. ICR mice were divided into four groups; normaldiet control, ethanol control (6.5g ethanol/kg), low-CNF (ethanol+150mg CNF/kg) and high-CNF (ethanol+300mg CNF/kg) groups. Consumption of alcohol for 8weeks induced severe liver damage with increases in prognostic indicators such as aspartate transaminase, alanine transaminase in serum whereas co-administration of CNF suppressed their increases. Excessive accumulations in liver TG and TC in ethanol control group were also suppressed by co-administration of CNF. Co-administration of CNF maintained SOD activity, GSH and malondialdehyde levels close to those of the normal diet group. Chronic consumption of alcohol also stimulated abrupt increases in pro-inflammatory cytokines such as nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver otherwise co-administration of CNF effectively suppressed production of these cytokines dose-dependently. These results indicate that co-administration of CNF with alcohol can alleviate alcohol induced liver damage through preventing lipid formation, protecting antioxidant system and suppressing productions of pro-inflammatory cytokines.
authors: Functional Materials Research Group, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea.
authors: Ho-Young
authors: HY
authors: Park
authors: Sang Keun
authors: SK
authors: Ha
authors: Hyojin
authors: H
authors: Eom
authors: Inwook
authors: I
authors: Choi
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002957:Citrus / Q000737:chemistry*; D004187:Disaccharides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D053583:Interleukin-1beta / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects; D008108:Liver Diseases, Alcoholic / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000096:biosynthesis; D009929:Organ Size / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D004187:Disaccharides; D044950:Flavanones; D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C500601:narirutin; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione