title: Adipogenic and osteogenic differentiation of Lin(-)CD271(+)Sca-1(+) adipose-derived stem cells.
abstract: Adipose-derived stem cells (ASCs) have been defined as cells that undergo sustained in vitro growth and have multilineage differentiation potential. However, the identity and purification of ASCs has proved elusive due to the lack of specific markers and poor understanding of their physiological roles. Here, we prospectively isolated and identified a restricted homogeneous subpopulation of ASCs (Lin(-)CD271(+)Sca-1(+)) from mouse adipose tissues on the basis of cell-surface markers. Individual ASCs generated colony-forming unit-fibroblast at a high frequency and could differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. Expansion of ASCs in a large quantity was feasible in medium supplemented with fibroblast growth factor-2 and leukemia inhibitory factor, without loss of adipogenic and osteogenic differentiation capacity. Moreover, we found that the transplanted ASCs can differentiate into adipocytes in adipogenic microenvironment in vivo and osteoblasts in osteogenic microenvironment in vivo. Thus we proved that Lin, CD271, and Sca-1 could be used as the specific markers to purify ASCs from adipose tissue. The method we established to identify ASCs as defined in vivo entities will help develop ASCs transplantation as a new therapeutic strategy for bone regeneration and adipose tissue regeneration in clinic.
authors: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
authors: Jingang
authors: J
authors: Xiao
authors: Xiaojuan
authors: X
authors: Yang
authors: Wei
authors: W
authors: Jing
authors: Weihua
authors: W
authors: Guo
authors: Qince
authors: Q
authors: Sun
authors: Yunfeng
authors: Y
authors: Lin
authors: Lei
authors: L
authors: Liu
authors: Wentong
authors: W
authors: Meng
authors: Weidong
authors: W
authors: Tian
mesh_terms: D050153:Abdominal Fat / Q000166:cytology; D000068816:Adapalene; D050156:Adipogenesis; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D001861:Bone Regeneration; D049109:Cell Proliferation; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000502:physiology; D005434:Flow Cytometry; D006882:Hydroxyapatites / Q000737:chemistry; D053605:Leukemia Inhibitory Factor / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009281:Naphthalenes / Q000378:metabolism*; D010012:Osteogenesis; D010641:Phenotype; D058985:Side-Population Cells / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000950:Antigens, Ly; D006882:Hydroxyapatites; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D009281:Naphthalenes; C074950:hydroxyapatite-beta tricalcium phosphate; D016222:Fibroblast Growth Factor 2; D000068816:Adapalene