title: Microbial synthesis of poly-3-hydroxybutyrate and its application as targeted drug delivery vehicle.
abstract: Arsenic trioxide loaded biocompatible PHB-PVA(1) nanoparticles (<100 nm in size) with folate functionalized surface were synthesized using poly-[(R)-3-hydroxybutyric acid] (PHB) produced by Bacillus firmus NII 0830. Folate functionalization was carried using dicyclohexyl carbodiimide (DCC) as a catalyst and 10-bromodecanol as a linker to conjugate glutamic acid terminal of folate with the hydroxylate groups present on the surface of PHBA-PVA(2) nanotrojans. The effect of fabrication parameters on shape, size distribution and PDI of the PHB nanoparticles were also investigated. It was observed that increase in sonication time and polyvinyl alcohol (PVA) concentration greatly reduced the size of nanoparticles. The drug release studies on arsenic trioxide incorporated PHB-PVA nanoparticles were conducted at physiological pH and temperature. FOL-PHBA-PVA(3) nanoparticles showed greater extent of cytotoxicity towards murine fibrosarcoma L929 cells than PHBA-PVA nanoparticles alone without conjugated folate, indicating the significance of folate as ligand for specific targeting of FR+ cancer cells.
authors: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Thiruvananthapuram, Kerala, India.
authors: Avanthi
authors: A
authors: Althuri
authors: Jincy
authors: J
authors: Mathew
authors: Raveendran
authors: R
authors: Sindhu
authors: Rintu
authors: R
authors: Banerjee
authors: Ashok
authors: A
authors: Pandey
authors: Parameswaran
authors: P
authors: Binod
mesh_terms: D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000008:administration & dosage / Q000378:metabolism*; D001407:Bacillus / Q000378:metabolism*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D058975:Folate Receptor 1 / Q000378:metabolism; D005492:Folic Acid / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000008:administration & dosage / Q000378:metabolism*; D010316:Particle Size; D011091:Polyesters / Q000378:metabolism*; D011142:Polyvinyl Alcohol; D000094122:Polyhydroxybutyrates
chemical_list: D000077237:Arsenic Trioxide; D001152:Arsenicals; D058975:Folate Receptor 1; D005492:Folic Acid; D006885:Hydroxybutyrates; D010087:Oxides; D011091:Polyesters; D011142:Polyvinyl Alcohol; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Arsenic trioxide loaded polyhydroxybutyrate–polyvinyl alcohol copolymer nanoparticles; Folate conjugated nanoparticles; Folate receptor positive; Polyhydroxybutyrate