title: Characterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa  burmeisteri and cross-species amplification.
abstract: Twelve polymorphic tetranucleotide microsatellite loci  were isolated and characterized for the leaf-frog Phyllomedusa burmeisteri,  an endemic species of the Brazilian Atlantic forest. These loci were screened  in 25 individuals from two populations of the Minas Gerais State (Carangola and  Juiz de Fora). The number of alleles per locus ranged from 3 to 16 (mean = 8).  Observed and expected heterozygosities ranged from 0.25 to 0.92 and 0.56 to  0.92, respectively. Evidence for both the presence of null alleles and Hardy-  Weinberg equilibrium deviations were found in loci Phybu4, Phybu17, and  Phybu21. Genotypic disequilibrium for each pair of loci across populations was  not significant. Cross-species amplification was successful for 11 of the 12  developed loci for the sister-species, P. bahiana. These microsatellites  will be important for future fine-scale population structure analyses.
authors: CIBIO/UP, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal. brunestuliana@gmail.com
authors: T O
authors: TO
authors: Brunes
authors: M S
authors: MS
authors: van de Vliet
authors: S
authors: S
authors: Lopes
authors: J
authors: J
authors: Alexandrino
authors: C F B
authors: CF
authors: Haddad
authors: F
authors: F
authors: Sequeira
mesh_terms: D000483:Alleles; D000818:Animals; D001001:Anura / Q000235:genetics*; D001938:Brazil; D005838:Genotype; D006579:Heterozygote; D018895:Microsatellite Repeats; D018515:Plant Leaves; D011110:Polymorphism, Genetic; D014197:Trees