title: Lactobacillus plantarum passage through an oro-gastro-intestinal tract simulator: carrier matrix effect and transcriptional analysis of genes associated to stress and probiosis.
abstract: Dietary probiotics should reach the intestine viable and in high numbers; therefore, they should tolerate the stress associated to the gastro-intestinal (GI) environment. Indeed, all along the different GI sections, probiotics are challenged by several sources of stress, including low pH, bile and digestive enzymes. Bacterial cells are equipped with various defense mechanisms to allow survival in hostile environments. The food matrix used to deliver beneficial bacteria may contribute to their probiotic action, e.g. by enhancing survival to stress and gut colonization. In this study, the survival of the lactic acid bacterium Lactobacillus plantarum WCFS1, a model probiotic strain, was examined in a human oro-gastric-intestinal (OGI) in vitro system, using different carrier matrices to compare protective and buffering properties. Higher survival was observed in complex and/or nutrient-rich matrices, and when potential prebiotics were added. The molecular response of L. plantarum to the OGI transit was analyzed by studying the transcriptional levels of genes involved in stress response and probiosis. The OGI steps of higher mortality corresponded to greater induction of stress genes, thus implying their involvement in adaptation to the gut environment. Plantaricins were significantly upregulated all along the different OGI sections; adhesion genes were mainly induced by gastric environment.
authors: Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Foggia, Viale Pinto, 1, 71122 Foggia, Italy.
authors: P
authors: P
authors: Bove
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy; Promis Biotech srl, spin-off of UNIFG, Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors: P
authors: P
authors: Russo
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy; Promis Biotech srl, spin-off of UNIFG, Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors: V
authors: V
authors: Capozzi
authors: Dipartimento di Scienze Mediche di base, Neuroscienze ed Organi di Senso, Università degli Studi di Bari "Aldo Moro", Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy.
authors: A
authors: A
authors: Gallone
authors: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy. Electronic address: g.spano@unifg.it.
authors: G
authors: G
authors: Spano
authors: Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Foggia, Viale Pinto, 1, 71122 Foggia, Italy.
authors: D
authors: D
authors: Fiocco
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D019936:Probiotics / Q000378:metabolism; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins