title: ECHS1 interacts with STAT3 and negatively regulates STAT3 signaling.
abstract: Signal transducer and activator of transcription 3 (STAT3) is a critical transcriptional factor in a variety of cellular processes, and is frequently over-activated in a range of human tumors. However, the processes that regulate STAT3 activation need to be further clarified. With a yeast two-hybrid screening, we identified enoyl-CoA hydratase short chain 1 (ECHS1) as a novel STAT3 binding protein. We further confirmed the interaction between STAT3 and ECHS1 by GST-pull down and co-immnunoprecipitation. Importantly, we found that ECHS1 specifically represses STAT3 activity and negatively regulates the expression of several target genes of STAT3 through inhibiting STAT3 phosphorylation. Therefore, our findings will provide new insights into the mechanism of STAT3 signaling regulation.
authors: Jilin University, Changchun 130000, China.
authors: Yan
authors: Y
authors: Chang
authors: Shao-Xin
authors: SX
authors: Wang
authors: Yu-Bo
authors: YB
authors: Wang
authors: Jie
authors: J
authors: Zhou
authors: Wei-Hua
authors: WH
authors: Li
authors: Na
authors: N
authors: Wang
authors: Di-Feng
authors: DF
authors: Fang
authors: Hui-Yan
authors: HY
authors: Li
authors: Ai-Ling
authors: AL
authors: Li
authors: Xue-Min
authors: XM
authors: Zhang
authors: Wei-Na
authors: WN
authors: Zhang
mesh_terms: D004746:Enoyl-CoA Hydratase / Q000235:genetics* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D015850:Interleukin-6 / Q000494:pharmacology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D050796:STAT3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D020798:Two-Hybrid System Techniques
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D007527:Isoenzymes; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D004815:Epidermal Growth Factor; D004746:Enoyl-CoA Hydratase