title: Comparative study of PCL-HAp and PCL-bioglass composite scaffolds for bone tissue engineering.
abstract: The aim of this work is to compare the effect of hydroxyapatite (HAp) or bioglass (BG) nanoparticles in a polycaprolactone composite scaffold aimed to bone regeneration. To allow a comparison of the influence of both types of fillers, scaffolds made of PCL or composites containing up to 20 % by weight HAp or BG were obtained. Scaffolds showed acceptable mechanical properties for its use and high interconnected porosity apt for cellular colonization. To study the effect of the different materials on pre-osteoblast cells differentiation, samples with 5 % mineral reinforcement, were cultured for up to 28 days in osteogenic medium. Cells proliferated in all scaffolds. Nevertheless, differentiation levels for the selected markers were higher in pure PCL scaffolds than in the composites; inclusion of bioactive particles showed no positive effects on cell differentiation. In osteogenic culture conditions, the presence of bioactive particles is thus not necessary in order to observe good differentiation.
authors: Center for Biomaterials and Tissue Engineering, Universidad Politécnica de Valencia, Edificio 8E, Esc F, Nivel 1, Camino de Vera s/n, 46022 Valencia, Spain. joaroroc@upvnet.upv.es
authors: Joaquín
authors: J
authors: Ródenas-Rochina
authors: José Luis Gómez
authors: JL
authors: Ribelles
authors: Myriam
authors: M
authors: Lebourg
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002516:Ceramics / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D019245:Compressive Strength; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D055119:Elastic Modulus; D008422:Materials Testing; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite