title: IL-17 induces expression of vascular cell adhesion molecule through signalling pathway of NF-κB, but not Akt1 and TAK1 in vascular smooth muscle cells.
abstract: Interleukin-17 (IL-17) plays an important role in several autoimmune diseases. IL-17 can induce the expression of vascular cell adhesion molecule (VCAM-1) in aortic vascular smooth muscle cells (SMCs), which is important for the development of atherosclerosis. However, the signalling pathway of IL-17-induced VCAM-1 expression remains unclear. In this study, we reported that IL-17-induced expression of VCAM-1 in SMCs is dependent on NF-κB, but independent of Akt1 and TAK1. This is because knocking down Akt1 or TAK1 by siRNA did not reduce IL-17-induced activation of NF-κB and expression of VCAM-1, whereas knocking down NF-κB by siRNA markedly inhibited IL-17-mediated upregulation of VCAM-1 expression. In addition, IL-17-induced expression of VCAM-1 is partially dependent on activation of ERK1/2. Therefore, these signalling pathways of IL-17-mediated upregulation of VCAM-1 expression might be therapeutic targets for treatment of IL-17-mediated inflammation.
authors: Arkansas Biosciences Institute, Department of Biological Science, Arkansas State University, Jonesboro, AR 72467, USA.
authors: H
authors: H
authors: Zhang
authors: J
authors: J
authors: Chen
authors: X
authors: X
authors: Liu
authors: L
authors: L
authors: Awar
authors: A
authors: A
authors: McMickle
authors: F
authors: F
authors: Bai
authors: S
authors: S
authors: Nagarajan
authors: S
authors: S
authors: Yu
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D020868:Gene Silencing; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D020381:Interleukin-17 / Q000494:pharmacology*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D048008:TNF Receptor-Associated Factor 3 / Q000235:genetics / Q000378:metabolism; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics*; D000099151:MAP Kinase Kinase Kinase 7
chemical_list: D018799:Intercellular Adhesion Molecule-1; D020381:Interleukin-17; D020930:MAP Kinase Kinase Kinases; D016328:NF-kappa B; D051057:Proto-Oncogene Proteins c-akt; D034741:RNA, Small Interfering; D048008:TNF Receptor-Associated Factor 3; D051996:Transcription Factor RelA; D019010:Vascular Cell Adhesion Molecule-1; D000099151:MAP Kinase Kinase Kinase 7; C494918:AKT1 protein, human