title: Nuclear recruitment assay as a tool to validate transcription factor interactions in Mammalian cells.
abstract: Identification and verification of novel transcription factor interactions is an inherent step in the discovery of molecular mechanisms driving gene transcription and regulation. Co-immunoprecipitation and GST-pull down are often key techniques in the verification process. Despite wide applicability, their use may sometimes be restricted. We provide a detailed protocol for an intracellular immunofluorescence technique that may be used as an alternative or complimentary study for transcription factor interaction verification.
authors: Heart Failure Research Center, Academic Medical Center, section K2, Amsterdam, The Netherlands.
authors: C J J
authors: CJ
authors: Boogerd
authors: V M
authors: VM
authors: Christoffels
authors: P
authors: P
authors: Barnett
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D008322:Mammals; D025941:Protein Interaction Mapping / Q000379:methods*; D021381:Protein Transport; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors