title: Enhanced transcutaneous immunization via dissolving microneedle array loaded with liposome encapsulated antigen and adjuvant.
abstract: Transcutaneous immunization (TCI) with dissolving microneedle arrays (DMAs) is a promising vaccine administration method. In this work, we developed a TCI device consisting of dissolving polyvinylpyrrolidone (PVP) microneedles array, where in the tips are loaded with antigen and adjuvant encapsulated in liposomes. The microneedles could effectively be inserted into the skin and completely dissolve within 3 min. As a test-case, we selected ovalbumin (OVA) as a model antigen, CpG OND as adjuvant and cationic liposome (Lip) as a microparticulate vehicle for co-deliver antigens and adjuvant. Mice were immunized transcutaneously with DMAs containing OVA, OVA-CpG OND, OVA encapsulated in Lip, OVA-CpG OND encapsulated in Lip and conventional intramuscular injection (IM) with OVA solution, respectively. The results show that the anti-OVA IgG antibody level in the group immunized with the DMA containing OVA-CpG OND encapsulated in Lip was significantly higher than that of the other groups. Furthermore, it significantly increased the level of IgG2a (P<0.05) and achieved the shift of immune type from predominate Th2 type to a balance Th1/Th2 type. In conclusion, the DMA TCI device can effectively deliver the Lip encapsulating CpG OND-OVA into skin, enhancing the immune response and change the immune type.
authors: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Lei
authors: L
authors: Guo
authors: Jianmin
authors: J
authors: Chen
authors: Yuqin
authors: Y
authors: Qiu
authors: Suohui
authors: S
authors: Zhang
authors: Bai
authors: B
authors: Xu
authors: Yunhua
authors: Y
authors: Gao
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000279:Administration, Cutaneous; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D007114:Immunization / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D008845:Microinjections; D009339:Needles; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology; D010316:Particle Size; D012995:Solubility; D013552:Swine
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D010047:Ovalbumin