title: Cell-scale dynamic recycling and cortical flow of the actin-myosin cytoskeleton for rapid cell migration.
abstract: Actin and myosin II play major roles in cell migration. Whereas pseudopod extension by actin polymerization has been intensively researched, less attention has been paid to how the rest of the actin cytoskeleton such as the actin cortex contributes to cell migration. In this study, cortical actin and myosin II filaments were simultaneously observed in migrating Dictyostelium cells under total internal reflection fluorescence microscopy. The cortical actin and myosin II filaments remained stationary with respect to the substratum as the cells advanced. However, fluorescence recovery after photobleaching experiments and direct observation of filaments showed that they rapidly turned over. When the cells were detached from the substratum, the actin and myosin filaments displayed a vigorous retrograde flow. Thus, when the cells migrate on the substratum, the cortical cytoskeleton firmly holds the substratum to generate the motive force instead. The present studies also demonstrate how myosin II localizes to the rear region of the migrating cells. The observed dynamic turnover of actin and myosin II filaments contributes to the recycling of their subunits across the whole cell and enables rapid reorganization of the cytoskeleton.
authors: Department of Functional Molecular Biology, Graduate School of Medicine, Yamaguchi University ,  Yamaguchi 753-8512 ,  Japan.
authors: Shigehiko
authors: S
authors: Yumura
authors: Go
authors: G
authors: Itoh
authors: Yumi
authors: Y
authors: Kikuta
authors: Takeomi
authors: T
authors: Kikuchi
authors: Toshiko
authors: T
authors: Kitanishi-Yumura
authors: Masatsune
authors: M
authors: Tsujioka
keywords: Actin; GFP; Myosin; Retrograde flow; TIRF