title: Mechanistic insights into the effect of nanoparticles on zebrafish hatch.
abstract: Aquatic organisms are susceptible to waterborne nanoparticles (NP) and there is only limited understanding of the mechanisms by which these emerging contaminants may affect biological processes. This study used silicon (nSi), cadmium selenide (nCdSe), silver (nAg) and zinc NPs (nZnO) as well as single-walled carbon nanotubes (SWCNT) to assess NP effects on zebrafish (Danio rerio) hatch. Exposure of 10 mg/L nAg and nCdSe delayed zebrafish hatch and 100 mg/L of nCdSe as well as 10 and 100 mg/L of uncoated nZnO completely inhibited hatch and the embryos died within the chorion. Both the morphology and the movement of the embryos were not affected, and it was determined that the main mechanism of hatch inhibition by NPs is likely through the interaction of NPs with the zebrafish hatching enzyme. Furthermore, it was concluded that the observed effects arose from the NPs themselves and not their dissolved metal components.
authors: Department of Biological Sciences, University of Alberta , Edmonton, AB , Canada.
authors: Kimberly Jessica
authors: KJ
authors: Ong
authors: Xinxin
authors: X
authors: Zhao
authors: Maria E
authors: ME
authors: Thistle
authors: Tyson J
authors: TJ
authors: Maccormack
authors: Rhett J
authors: RJ
authors: Clark
authors: Guibin
authors: G
authors: Ma
authors: Yadienka
authors: Y
authors: Martinez-Rubi
authors: Benoit
authors: B
authors: Simard
authors: Joachim Say Chye
authors: JS
authors: Loo
authors: Jonathan G C
authors: JG
authors: Veinot
authors: Greg G
authors: GG
authors: Goss
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D053768:Metal Nanoparticles / Q000633:toxicity*; D019216:Metals, Heavy / Q000633:toxicity*; D037742:Nanotubes, Carbon / Q000633:toxicity; D010447:Peptide Hydrolases / Q000378:metabolism; D012825:Silicon / Q000633:toxicity; D015027:Zebrafish / Q000502:physiology*
chemical_list: D019216:Metals, Heavy; D037742:Nanotubes, Carbon; D010447:Peptide Hydrolases; D012825:Silicon