title: Cleavage-based signal amplification of RNA.
abstract: RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme and signal amplification. Cleavage-based signal amplification of RNA cannot be contaminated by genomic DNA and is suitable for the detection of both mRNA and microRNA targets, with high specificity and sensitivity. Moreover, the detection results can be reported in a colorimetric or real-time fluorometric way for different detection purposes.
authors: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors: Yongyun
authors: Y
authors: Zhao
authors: Li
authors: L
authors: Zhou
authors: Zhuo
authors: Z
authors: Tang
mesh_terms: D001483:Base Sequence; D003124:Colorimetry; D003199:Computer Systems; D021881:DNA, Catalytic / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005453:Fluorescence; D005470:Fluorometry; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D015203:Reproducibility of Results; D013379:Substrate Specificity
chemical_list: D021881:DNA, Catalytic; C034477:RNA primers; D012313:RNA; D004259:DNA-Directed DNA Polymerase