title: Quantitative proteomic analysis reveals the neuroprotective effects of huperzine A for amyloid beta treated neuroblastoma N2a cells.
abstract: Alzheimer's disease is a worldwide metabolic disease and an economically costly disease to society, so more medicines need to be developed to treat this disease. Huperzine A, a novel lycopodium alkaloid isolated from traditional Chinese medicine Huperzia serrata (Qian Ceng Ta), has been shown to possess multiple neuroprotective effects for Alzheimer's disease, but the precise pharmacological mechanism of huperzine A is unclear and needs to be further investigated. In this study, proteins from untreated N2a cells (Con group), cells preincubated with huperzine A followed by Aβ (1-42) oligomers treatment (HupA group) and cells treated with Aβ (1-42) oligomers (Aβ group) with five biological replicates in each cohort, were processed in a centrifugal proteomic reactor and quantified by label-free quantitation. A total of 2860 proteins were quantified with high confidence, and 198 proteins were significantly changed (with p-value < 0.05) between HupA and Aβ cohorts. The pathway and direct protein-protein interaction network analysis showed that huperzine A protects N2a cells against Aβ oligomer-induced cell death by downregulation of cellular tumor antigen p53 (Trp53) expression.
authors: CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors: Yimin
authors: Y
authors: Tao
authors: Li
authors: L
authors: Fang
authors: Yiming
authors: Y
authors: Yang
authors: Hualiang
authors: H
authors: Jiang
authors: Huaiyu
authors: H
authors: Yang
authors: Haiyan
authors: H
authors: Zhang
authors: Hu
authors: H
authors: Zhou
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000544:Alzheimer Disease / Q000188:drug therapy / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D030562:Databases, Protein; D015536:Down-Regulation / Q000187:drug effects; D051379:Mice; D009447:Neuroblastoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D060066:Protein Interaction Maps; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000470:Alkaloids; D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D012717:Sesquiterpenes; D016159:Tumor Suppressor Protein p53; C075222:amyloid beta-protein (1-42); C050426:huperzine A