title: Microarray analysis of gene expression profile in resistant and susceptible Bombyx mori strains reveals resistance-related genes to nucleopolyhedrovirus.
abstract: To investigate the molecular mechanism of silkworm resistance to BmNPV infection, we constructed a near-isogenic line (BC8) with BmNPV resistance using highly resistant (NB) and highly susceptible parental strains (306). We investigated variations in the gene expression in the midguts of BmNPV-infected BC8 and 306 at 12 h pi using the microarray. 92 differentially expressed genes were identified. Real-time qPCR analysis confirmed that 10 genes were significantly up-regulated or down-regulated in the midguts of BC8 and NB compared to 306. To our knowledge, we first defined the role of the amino acid transporter and 26S proteasome in insect antiviral. However, serine protease was not completely consistent with data of reported previously in insect antiviral. The role of the 5 genes (Bm123, Bm122, COP β', aquaporin, glycoside hydrolases) was also demonstrated in insect antiviral. Our results provided new insights into the molecular mechanism of the Bombyx mori immune response against BmNPV infection.
authors: Institute of Life Sciences, Jiangsu University, China.
authors: Yang
authors: Y
authors: Zhou
authors: Lu
authors: L
authors: Gao
authors: Haifeng
authors: H
authors: Shi
authors: Hengchuan
authors: H
authors: Xia
authors: Li
authors: L
authors: Gao
authors: Chaoqun
authors: C
authors: Lian
authors: Liang
authors: L
authors: Chen
authors: Qin
authors: Q
authors: Yao
authors: Keping
authors: K
authors: Chen
authors: Xiaoyong
authors: X
authors: Liu
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017344:Genes, Insect; D017924:Nucleopolyhedroviruses; D020411:Oligonucleotide Array Sequence Analysis; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D057057:Serine Proteases / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D026905:Amino Acid Transport Systems; D057057:Serine Proteases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease