title: N-glycoproteome of E14.Tg2a mouse embryonic stem cells.
abstract: E14.Tg2a mouse embryonic stem (mES) cells are a widely used host in gene trap and gene targeting techniques. Molecular characterization of host cells will provide background information for a better understanding of functions of the knockout genes. Using a highly selective glycopeptide-capture approach but ordinary liquid chromatography coupled mass spectrometry (LC-MS), we characterized the N-glycoproteins of E14.Tg2a cells and analyzed the close relationship between the obtained N-glycoproteome and cell-surface proteomes. Our results provide a global view of cell surface protein molecular properties, in which receptors seem to be much more diverse but lower in abundance than transporters on average. In addition, our results provide a systematic view of the E14.Tg2a N-glycosylation, from which we discovered some striking patterns, including an evolutionarily preserved and maybe functionally selected complementarity between N-glycosylation and the transmembrane structure in protein sequences. We also observed an environmentally influenced N-glycosylation pattern among glycoenzymes and extracellular matrix proteins. We hope that the acquired information enhances our molecular understanding of mES E14.Tg2a as well as the biological roles played by N-glycosylation in cell biology in general.
authors: Institute for Systems Biology, Seattle, Washington, United States of America. bingyun_sun@sfu.ca
authors: Bingyun
authors: B
authors: Sun
authors: Li
authors: L
authors: Ma
authors: Xiaowei
authors: X
authors: Yan
authors: Denis
authors: D
authors: Lee
authors: Vinita
authors: V
authors: Alexander
authors: Laura J
authors: LJ
authors: Hohmann
authors: Cynthia
authors: C
authors: Lorang
authors: Lalangi
authors: L
authors: Chandrasena
authors: Qiang
authors: Q
authors: Tian
authors: Leroy
authors: L
authors: Hood
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006020:Glycopeptides / Q000032:analysis* / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006031:Glycosylation; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins; D008565:Membrane Proteins; D020543:Proteome