title: Retrovirus-mediated transduction of a cytosine deaminase gene preserves the                     stemness of mesenchymal stem cells.
abstract: Human mesenchymal stem cells (MSCs) have emerged as attractive cellular vehicles                     to deliver therapeutic genes for ex-vivo therapy of diverse diseases;                     this is, in part, because they have the capability to migrate into tumor or                     lesion sites. Previously, we showed that MSCs could be utilized to deliver a                     bacterial cytosine deaminase (CD) suicide gene to brain tumors. Here we                     assessed whether transduction with a retroviral vector encoding CD gene                     altered the stem cell property of MSCs. MSCs were transduced at passage 1 and                     cultivated up to passage 11. We found that proliferation and differentiation                     potentials, chromosomal stability and surface antigenicity of MSCs were not                     altered by retroviral transduction. The results indicate that retroviral vectors                     can be safely utilized for delivery of suicide genes to MSCs for                         ex-vivo therapy. We also found that a single retroviral                     transduction was sufficient for sustainable expression up to passage 10. The                     persistent expression of the transduced gene indicates that transduced MSCs                     provide a tractable and manageable approach for potential use in allogeneic                     transplantation.
authors: Department of Anatomy, Ajou University School of Medicine, Suwon, South Korea.
authors: Jin Sung
authors: JS
authors: Park
authors: Da-Young
authors: DY
authors: Chang
authors: Ji-Hoi
authors: JH
authors: Kim
authors: Jin Hwa
authors: JH
authors: Jung
authors: JoonSeong
authors: J
authors: Park
authors: Se-Hyuk
authors: SH
authors: Kim
authors: Young-Don
authors: YD
authors: Lee
authors: Sung-Soo
authors: SS
authors: Kim
authors: Haeyoung
authors: H
authors: Suh-Kim
mesh_terms: D000293:Adolescent; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology; D002648:Child; D043525:Cytosine Deaminase / Q000235:genetics* / Q000627:therapeutic use; D005472:Fluorouracil / Q000494:pharmacology; D015316:Genetic Therapy; D042822:Genomic Instability / Q000187:drug effects; D006801:Humans; D059785:Karyotype; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009369:Neoplasms / Q000628:therapy; D012190:Retroviridae / Q000378:metabolism*; D013997:Time Factors; D014161:Transduction, Genetic
chemical_list: D043525:Cytosine Deaminase; D005472:Fluorouracil