title: Evaluation of the antihypertensive properties of yellow passion fruit pulp (Passiflora edulis Sims f. flavicarpa Deg.) in spontaneously hypertensive rats.
abstract: Various species of the genus Passiflora have been extensively used in traditional medicine as sedatives, anxiolytics, diuretics and analgesics. In the present study, after the identification and quantification of phytochemical compounds from yellow passion fruit pulp by liquid chromatography-photodiode array-mass spectrometry (HPLC-PDA-MS/MS), its antihypertensive effect was investigated on spontaneously hypertensive rats. Additionally, the renal function, evaluated by kidney/body weight, serum creatinine, proteinuria, urinary flow, reduced glutathione (GSH) levels and thiobarbituric acid-reactive substances (TBARS) and mutagenicity in bone marrow cells were assessed to evaluate the safety of passion fruit consumption. Yellow passion fruit pulp (5, 6 or 8 g/kg b.w.) was administered by gavage once a day for 5 consecutive days. HLPC-PDA-MS/MS analysis revealed that yellow passion fruit pulp contains phenolic compounds, ascorbic acid, carotenoids and flavonoids. The highest dose of passion fruit pulp significantly reduced the systolic blood pressure, increased the GSH levels and decreased TBARS. There were no changes in renal function parameters or the frequency of micronuclei in bone marrow cells. In conclusion, the antihypertensive effect of yellow passion fruit pulp, at least in part, might be due to the enhancement of the antioxidant status. The exact mechanisms responsible by this effect need further investigation.
authors: Departamento de Alimentos e Nutrição, Faculdade de Ciência Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, SP, Brazil.
authors: Eliziane Mieko
authors: EM
authors: Konta
authors: Mara Ribeiro
authors: MR
authors: Almeida
authors: Cátia Lira
authors: CL
authors: do Amaral
authors: Joana Darc Castania
authors: JD
authors: Darin
authors: Veridiana V
authors: VV
authors: de Rosso
authors: Adriana Zerlotti
authors: AZ
authors: Mercadante
authors: Lusânia Maria Greggi
authors: LM
authors: Antunes
authors: Maria Lourdes Pires
authors: ML
authors: Bianchi
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000737:chemistry; D001794:Blood Pressure / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D002338:Carotenoids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000097:blood; D005419:Flavonoids / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D006973:Hypertension / Q000188:drug therapy*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D029598:Passiflora / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000959:Antihypertensive Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D017392:Thiobarbituric Acid Reactive Substances; D002338:Carotenoids; D003404:Creatinine; D005978:Glutathione; D001205:Ascorbic Acid
keywords: carotenoids; hypertension; micronuclei; passion fruit; phenolic compounds; renal function