title: Effect of cordycepin on the expression of the inflammatory cytokines TNF-alpha, IL-6, and IL-17A in C57BL/6 mice.
abstract: Culture supernatants of splenocytes from C57BL/6 mice were exposed to 0.3, 1.0, and 3.0 microg/ml cordycepin plus 3.0 microg/ml lipopolysaccharide (LPS) to investigate the effects of cordycepin (3'-deoxyadenosine) on the production of inflammatory cytokines. Co-administration of 3.0 microg/ml cordycepin with LPS in cultured murine spleen cells significantly diminished the expression of the inflammatory cytokines tumor necrosis factor-alpha and interleukin-6 (IL-6) in a time-dependent manner. Expression of the inflammatory cytokine IL-17A was substantially down-regulated in a time- dependent manner at all cordycepin concentrations. These findings suggest that cordycepin down-regulates the immediate hypersensitivity reaction stimulated by LPS.
authors: Department of Biotechnology, Dong-A University, Busan, Korea.
authors: Min Jeong
authors: MJ
authors: Seo
authors: Min Jeong
authors: MJ
authors: Kim
authors: Hye Hyeon
authors: HH
authors: Lee
authors: Jeong Uck
authors: JU
authors: Park
authors: Byoung Won
authors: BW
authors: Kang
authors: Gi-Young
authors: GY
authors: Kim
authors: En Ju
authors: EJ
authors: Rhu
authors: Jung-In
authors: JI
authors: Kim
authors: Kwang Hyuk
authors: KH
authors: Kim
authors: Yong Kee
authors: YK
authors: Jeong
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003839:Deoxyadenosines / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000378:metabolism*; D020381:Interleukin-17 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D003839:Deoxyadenosines; D007166:Immunosuppressive Agents; D020381:Interleukin-17; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; C058120:cordycepin