title: [Study of detection of SPAD value in tomato leaves stressed by grey mold based on hyperspectral technique].
abstract: Hyperspectral imaging feature of chlorophyll content (SPAD) in tomato leaves stressed by grey mold was studied in the present paper. Hyperspectral imagings of healthy and infected tomato leaves were obtained by hyperspectral imaging system from 380 to 1 030 nm and diffuse spectral response of region of interest (ROI) from hyperspectral imaging was extracted by EN-VI software, then different preprocessing methods were used including smoothing and normalization etc. The partial least squares regress (PLSR) and principal component regress (PCR) models were developed for the prediction of SPAD value in tomato leaves based on normalization preprocessing method, then the back-propagation neural network (BPNN) and least squares-support vector machine (LS-SVM) models were built based on the four variables suggested by PLSR model. Among the four models, LS-SVM model was the best to predict SPAD value and the coefficient of determination (R2) was 0.901 8 with the root mean square error of prediction (RMSEP) of 2.599 2. It was demonstrated that chlorophyll content (SPAD) in healthy and infected tomato leaves can be effectively detected by the hyperspectral imaging technique.
authors: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. cqxie@zju.edu.cn
authors: Chuan-Qi
authors: CQ
authors: Xie
authors: Yong
authors: Y
authors: He
authors: Xiao-Li
authors: XL
authors: Li
authors: Fei
authors: F
authors: Liu
authors: Peng-Peng
authors: PP
authors: Du
authors: Lei
authors: L
authors: Feng
mesh_terms: D002734:Chlorophyll / Q000032:analysis*; D016018:Least-Squares Analysis; D018551:Solanum lycopersicum / Q000378:metabolism / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000737:chemistry* / Q000378:metabolism; D025341:Principal Component Analysis; D012044:Regression Analysis; D013057:Spectrum Analysis / Q000379:methods*; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D002734:Chlorophyll