title: Detection of human cytomegalovirus in transbronchial biopsies from lung transplant recipients.
abstract: The role of human cytomegalovirus (HCMV) in lung transplantation (LT) and drawbacks related to viral quantification in bronchoalveolar lavage (BAL) underline the potential usefulness of investigating other specimens. Thirty-three LT recipients were prospectively studied by HCMV quantitative real time PCR on matched transbronchial biopsy (TBB), BAL, and whole blood specimens. Overall, 27/33 patients turned out HCMV-positive in at least one specimen: 7.1 %, 37.1 %, and 13.5 % of TBB, BAL, and blood samples, respectively. No significant association between HCMV on all types of specimens and acute rejection, lymphocytic bronchiolitis, bronchiolitis obliterans and bronchiolitis obliterans syndrome was found. HCMV pneumonia was associated to HCMV detection on TBB (p = 0.003) and whole blood (p = 0.008), not on BAL (p = 0.47). The highest mean viral load was detected in TBB from cases with HCMV pneumonia in comparison to all other cases, suggesting the potential use of HCMV investigation in TBB for evaluating posttransplant complications.
authors: Virology Unit, Azienda Ospedaliera Città della Salute e della Scienza di Torino, Hospital San Giovanni Battista, University Hospital San Giovanni Battista di Torino, via Santena 9, Turin, Italy. cristina.costa@unito.it
authors: Cristina
authors: C
authors: Costa
authors: Antonio
authors: A
authors: Curtoni
authors: Francesca
authors: F
authors: Sidoti
authors: Cinzia
authors: C
authors: Balloco
authors: Salvatore
authors: S
authors: Simeone
authors: Samantha
authors: S
authors: Mantovani
authors: Elsa
authors: E
authors: Piasentin Alessio
authors: Daniela
authors: D
authors: Libertucci
authors: Luisa
authors: L
authors: Delsedime
authors: Paolo
authors: P
authors: Solidoro
authors: Sergio
authors: S
authors: Baldi
authors: Rossana
authors: R
authors: Cavallo
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy / Q000379:methods*; D001769:Blood / Q000821:virology; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D019411:Clinical Laboratory Techniques / Q000379:methods; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology*; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008168:Lung / Q000821:virology*; D008297:Male; D008875:Middle Aged; D011024:Pneumonia, Viral / Q000175:diagnosis / Q000821:virology*; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D014180:Transplantation; D019562:Viral Load; D014773:Virology / Q000379:methods