title: Cloning of a novel feruloyl esterase gene from rumen microbial metagenome and enzyme characterization in synergism with endoxylanases.
abstract: A feruloyl esterase (FAE) gene was isolated from a rumen microbial metagenome, cloned into E. coli, and expressed in active form. The enzyme (RuFae2) was identified as a type C feruloyl esterase. The RuFae2 alone released ferulic acid from rice bran, wheat bran, wheat-insoluble arabinoxylan, corn fiber, switchgrass, and corn bran in the order of decreasing activity. Using a saturating amount of RuFae2 for 100 mg substrate, a maximum of 18.7 and 80.0 μg FA was released from 100 mg corn fiber and wheat-insoluble arabinoxylan, respectively. Addition of GH10 endoxylanase (EX) synergistically increased the release of FA with the highest level of 6.7-fold for wheat bran. The synergistic effect of adding GH11 EX was significantly smaller with all the substrates tested. The difference in the effect of the two EXs was further analyzed by comparing the rate in the release of FA with increasing EX concentration using wheat-insoluble arabinoxylan as the substrate.
authors: Western Regional Research Center, USDA-ARS, Albany, CA, USA. dominic.wong@ars.usda.gov
authors: Dominic W S
authors: DW
authors: Wong
authors: Victor J
authors: VJ
authors: Chan
authors: Hans
authors: H
authors: Liao
authors: Mary J
authors: MJ
authors: Zidwick
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D003001:Cloning, Molecular; D003373:Coumaric Acids / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D054892:Metagenome; D008969:Molecular Sequence Data; D012417:Rumen / Q000382:microbiology*; D014908:Triticum / Q000378:metabolism; D014990:Xylans / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism
chemical_list: D003373:Coumaric Acids; D004043:Dietary Fiber; D014990:Xylans; C085118:arabinoxylan; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D043364:Endo-1,4-beta Xylanases