title: Comparative analysis of viral concentration methods for detecting the HAV genome using real-time RT-PCR amplification.
abstract: Hepatitis A is a major infectious disease epidemiologically associated with foodborne and waterborne outbreaks. Molecular detection using real-time RT-PCR to detect the hepatitis A virus (HAV) in contaminated vegetables can be hindered by low-virus recoveries during the concentration process and by natural PCR inhibitors in vegetables. This study evaluated three virus concentration methods from vegetables: polyethylene glycol (PEG) precipitation, ultrafiltration (UF), and immunomagnetic separation (IMS). UF was the most efficient concentration method, while PEG and IMS were very low for the recovery rate of HAV. These results demonstrate that UF is the most appropriate method for recovering HAV from contaminated vegetables and that this method combined with the real-time RT-PCR assay may be suitable for routine laboratory use.
authors: Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, 72-1, Nae-ri, Daeduck-myun, Ansung-si, Kyounggi-do, 456-756, South Korea.
authors: Kang Bum
authors: KB
authors: Lee
authors: Hyeokjin
authors: H
authors: Lee
authors: Sang-Do
authors: SD
authors: Ha
authors: Doo-Sung
authors: DS
authors: Cheon
authors: Changsun
authors: C
authors: Choi
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D004196:Disease Outbreaks; D005374:Filtration / Q000379:methods*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000821:virology; D016679:Genome, Viral; D006506:Hepatitis A / Q000821:virology*; D030041:Hepatitis A virus; D006801:Humans; D008280:Magnetics / Q000379:methods; D011092:Polyethylene Glycols; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014675:Vegetables / Q000821:virology*
chemical_list: D004279:DNA, Viral; D011092:Polyethylene Glycols