title: Prostate cancer-specific thermo-responsive polymer-coated iron oxide nanoparticles.
abstract: Thermo-responsive poly(N-isopropylacrylamide-acrylamide-allylamine)-coated magnetic nanoparticles (PMNPs) were developed and conjugated with prostate cancer-specific R11 peptides for active targeting and imaging of prostate cancer. The stable nanoparticles with an average diameter of 100 nm and surface charge of -27.0 mV, had a lower critical solution temperature of 40 °C. Magnetic characterization showed that the nanoparticles can be recruited using a magnetic field and possess superparamagnetic behavior even after R11 conjugation. In vitro cell studies demonstrated that R11-conjugated PMNPs (R11-PMNPs) were compatible with human dermal fibroblasts and normal prostate epithelial cells to all tested concentrations up to 500 μg/ml after 24 h of incubation. Moreover, the nanoparticles were taken up by prostate cancer cells (PC3 and LNCaP) in a dose-dependent manner, which was higher in case of R11-PMNPs than PMNPs. Further, in vivo biodistribution of the nanoparticles showed significantly more R11-PMNPs accumulation in tumors than other vital organs unlike PMNPs without R11 conjugation. Moreover, R11-PMNPs decreased 30% magnetic resonance T2 signal intensity in tumors in vivo compared to 0% decrease with PMNPs. These results indicate great potential of R11-PMPs as platform technology to target and monitor prostate cancers for diagnostic and therapeutic applications.
authors: Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA. aniket.wadajkar@mavs.uta.edu
authors: Aniket S
authors: AS
authors: Wadajkar
authors: Jyothi U
authors: JU
authors: Menon
authors: Yuh-Shyan
authors: YS
authors: Tsai
authors: Crystal
authors: C
authors: Gore
authors: Timothy
authors: T
authors: Dobin
authors: Leah
authors: L
authors: Gandee
authors: Kim
authors: K
authors: Kangasniemi
authors: Masaya
authors: M
authors: Takahashi
authors: Bikash
authors: B
authors: Manandhar
authors: Jung-Mo
authors: JM
authors: Ahn
authors: Jer-Tsong
authors: JT
authors: Hsieh
authors: Kytai T
authors: KT
authors: Nguyen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D011108:Polymers / Q000737:chemistry* / Q000627:therapeutic use*; D011471:Prostatic Neoplasms / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D005290:Ferric Compounds; D011108:Polymers; C000499:ferric oxide