title: Direct electrochemistry and electrocatalytic behavior of hemoglobin entrapped in Ag@C nanocables/gold nanoparticles nanocomposites film.
abstract: Direct electrochemistry of hemoglobin (Hb) was successfully fabricated by immobilizing Hb on the nanocomposites containing of Ag@C nanocables and Au nanoparticles (AuNPs) modified glassy carbon electrode (GCE). The immobilized Hb retained its biological activity and shown high catalytic activities to the reduction of H2O2 by circular dicroism (CD) spectrum, fourier transform infrared (FT-IR) spectrum and cyclic voltammetry (CV). Experimental conditions such as scan rate and pH Value were studied and optimized. The results indicated that the resulting biosensor are linear to the concentrations of H2O2 in the ranges of 6.67 x 10(-7)-2.40 x 10(5) M, and the detection limit is 2.02 x 10(-7) M. The electrochemical biosensor has also high stability and good reproducibility.
authors: School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, China.
authors: Xiao-Wei
authors: XW
authors: Hu
authors: Chang-Jie
authors: CJ
authors: Mao
authors: Ji-Ming
authors: JM
authors: Song
authors: He-Lin
authors: HL
authors: Niu
authors: Sheng-Yi
authors: SY
authors: Zhang
authors: Rong-Jing
authors: RJ
authors: Cui
mesh_terms: D002384:Catalysis; D002942:Circular Dichroism; D004563:Electrochemistry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D006454:Hemoglobins; D006046:Gold; D006861:Hydrogen Peroxide