title: Cyto-genotoxic and oxidative effects of a continuous UV-C treatment of liquid egg products.
abstract: UV-C treatment of food is a promising non-thermal processing technology to improve food safety and preservation. Most of the chemical constituents of food absorb UV-C light that can lead to chemical modifications and quality changes. This work investigated the effects of UV-C treatment of liquid egg products on lipid, protein oxidations and potential cyto- and genotoxic effects on intestinal epithelial cells in vitro. Egg preparations (egg white, yolk, liquid whole egg) were treated with UV-C (254 nm, volumetric doses between 0 and 115,619 J L(-1)) using a commercial UV-C processing unit equipped with a Dean Flow reactor. UV-C treatment at high doses (from 32,181 J L(-1), about 2 times higher than that needed to inactivate 5 log of relevant microorganisms) showed an increased lipid oxidation in egg yolk and slight effects in liquid whole eggs; this was confirmed by slightly but not statistically significant increased peroxide values. UV-C induced also slight protein damage, characterised by the total sulfhydryl group reduction. These UV-C-induced oxidative modifications in egg preparations however did not cause any increase in the cyto- or genotoxic (DNA strand breaks) effects in intestinal Caco-2 cells.
authors: Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Insvestigaciones Cientificas, Department of Conservation and Quality, Avda. Agustín Escardino 7, 46980 Paterna, Spain. poli.souza@iata.csic.es
authors: Poliana
authors: P
authors: Mendes de Souza
authors: Karlis
authors: K
authors: Briviba
authors: Alexandra
authors: A
authors: Müller
authors: Avelina
authors: A
authors: Fernández
authors: Mario
authors: M
authors: Stahl
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development / Q000528:radiation effects; D018938:Caco-2 Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D020552:Comet Assay; D003603:Cytotoxins; D053960:DNA Breaks / Q000187:drug effects; D004527:Egg Proteins / Q000737:chemistry* / Q000633:toxicity; D004531:Eggs / Q000032:analysis* / Q000528:radiation effects*; D005260:Female; D005514:Food Irradiation / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000737:chemistry; D010084:Oxidation-Reduction; D014466:Ultraviolet Rays
chemical_list: D003603:Cytotoxins; D004527:Egg Proteins; D008055:Lipids