title: Adjuvanticity of a recombinant calreticulin fragment in assisting anti-β-glucan IgG responses in T cell-deficient mice.
abstract: Polysaccharide-encapsulated fungi are the chief source of diseases in immunocompromised hosts such as those infected with human immunodeficiency virus or neutropenia patients. Currently available polysaccharide-protein conjugate vaccines are mainly T cell dependent and are usually ineffective in weakened immune systems. In this study, laminarin, a well-characterized β-1,3-glucan, was conjugated with a prokaryotically expressed recombinant fragment (amino acids [aa] 39 to 272) of calreticulin (rCRT/39-272), which exhibits extraordinarily potent immunogenicity and adjuvanticity in experimental animals. The resultant conjugate reserves the immunostimulatory effect of rCRT/39-272 on naïve murine B cells and is capable of eliciting anti-β-glucan IgG (mostly IgG1) responses in not only BALB/c mice but also athymic nude mice. Laminarin-CRT-induced mouse antibodies (Abs) are able to bind with Candida albicans and inhibit its growth in vitro. In addition, vaccination with laminarin-CRT partially protects mice from lethal C. albicans challenge. These results imply that rCRT/39-272 could be used as an ideal carrier or adjuvant for carbohydrate vaccines aimed at inducing or boosting IgG responses to fungal infections in immunodeficient hosts.
authors: Department of Immunology, Peking University Health Science Center, Beijing, China.
authors: Wei-Ji
authors: WJ
authors: Li
authors: Kai
authors: K
authors: Long
authors: Hong-Liang
authors: HL
authors: Dong
authors: Xiao-Ming
authors: XM
authors: Gao
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D037282:Calreticulin / Q000235:genetics / Q000378:metabolism*; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology; D002177:Candidiasis / Q000276:immunology / Q000517:prevention & control; D005260:Female; D005657:Fungal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005936:Glucans; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011134:Polysaccharides / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016019:Survival Analysis; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000908:Antibodies, Fungal; D037282:Calreticulin; D005657:Fungal Vaccines; D005936:Glucans; D007074:Immunoglobulin G; D011134:Polysaccharides; D011994:Recombinant Proteins; D018074:Vaccines, Conjugate; D014614:Vaccines, Synthetic; C008247:laminaran