title: Human immunodeficiency viruses: propagation, quantification, and storage.
abstract: Described in this unit are basic protocols frequently used in the research of human immunodeficiency viruses (HIVs). Provided are methods for propagating and quantifying HIV, as well as recommendations for long-term storage. Background information about these methods is also provided and includes their advantages, disadvantages, and troubleshooting.
authors: Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors: Paul J
authors: PJ
authors: Peters
authors: Kathryn
authors: K
authors: Richards
authors: Paul
authors: P
authors: Clapham
mesh_terms: D002460:Cell Line; D015497:HIV-1 / Q000254:growth & development* / Q000302:isolation & purification*; D015498:HIV-2 / Q000254:growth & development* / Q000302:isolation & purification*; D006801:Humans; D011309:Preservation, Biological / Q000379:methods*; D019562:Viral Load; D014776:Virus Cultivation / Q000379:methods