title: Microwave irradiation as an alternative method for disinfection of denture base acrylic resins.
abstract: OBJECTIVE
  This study evaluated the effect of microwave irradiation as an alternative method for disinfection of different types of denture base acrylic resins.
  
  
  METHODS
  Twenty-four samples for each conventional, microwaved and characterized heat-cured acrylic resin were made and subjected to sterilization with ethylene oxide for the groups: 1) irradiated samples; 2) non-irradiated samples; and 3) samples without yeast. Each group was subdivided according to inoculation with C. albicans, C. dubliniensis and C. tropicalis. The samples were inoculated with 100 µL of inoculum of each species of Candida and later placed in an incubator at 37 °C for 1 hr to perform the first adhesion. After this time, each well was supplemented with sterile media and the plate was once again taken to a stove for incubation at 37 °C for 6 hr. The samples were immersed in 100 mL of sterile water and irradiated with microwave at 650 W for 3 min. Control samples were considered as the non-irradiated group. After incubation for 48 hr, irradiated and non-irradiated samples were subjected to a digital colony counter.
  
  
  RESULTS
  Control group (non-irradiated) showed microbial growth for resins and the means of ufc/mL were without statistically significant differences. Microwave irradiated samples (experimental group) promoted no viable colonies for all Candida species and types of acrylic resins. The means of ufc/mL were without statistically significant differences.
  
  
  CONCLUSIONS
  Microwave irradiation was an effective method for disinfection of the acrylic resins inoculated with C. albicans, C. dubliniensis and C. tropicalis.
authors: Piracicaba Dental School, UNICAMP, Piracicaba, Brazil.
authors: M
authors: M
authors: Silva
authors: R
authors: R
authors: Consani
authors: J
authors: J
authors: Sardi
authors: M
authors: M
authors: Mesquita
authors: A
authors: A
authors: Macêdo
authors: J
authors: J
authors: Takahashi
mesh_terms: D000180:Acrylic Resins / Q000528:radiation effects*; D002175:Candida / Q000254:growth & development / Q000528:radiation effects*; D002176:Candida albicans / Q000254:growth & development / Q000528:radiation effects; D041022:Candida tropicalis / Q000254:growth & development / Q000528:radiation effects; D002177:Candidiasis / Q000517:prevention & control; D017266:Dental Prosthesis; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination; D005027:Ethylene Oxide; D006358:Hot Temperature; D006801:Humans; D008872:Microwaves; D009894:Opportunistic Infections / Q000517:prevention & control; D016459:Prosthesis-Related Infections / Q000517:prevention & control
chemical_list: D000180:Acrylic Resins; D005027:Ethylene Oxide