title: Immunosensor based on immobilization of antigenic peptide NS5A-1 from HCV and silk fibroin in nanostructured films.
abstract: The peptide NS5A-1 (PPLLESWKDPDYVPPWHG), derived from hepatitis C virus (HCV) NS5A protein, was immobilized into layer-by-layer (LbL) silk fibroin (SF) films. Deposition was monitored by UV-vis absorption measurements at each bilayer deposited. The interaction SF/peptide film induced secondary structure in NS5A-1 as indicated by fluorescence and circular dichroism (CD) measurements. Voltammetric sensor (SF/NS5A-1) properties were observed when the composite film was tested in the presence of anti-HCV. The peptide-silk fibroin interaction studied here showed new architectures for immunosensors based on antigenic peptides and SF as a suitable immobilization matrix.
authors: Institute of Chemistry, São Paulo State University, UNESP, CP355-Araraquara-SP, 14801-970 Brazil. marli.moraes@gmail.com
authors: Marli L
authors: ML
authors: Moraes
authors: Lais R
authors: LR
authors: Lima
authors: Robson R
authors: RR
authors: Silva
authors: Mauricio
authors: M
authors: Cavicchioli
authors: Sidney J L
authors: SJ
authors: Ribeiro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D005349:Fibroins / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000737:chemistry*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D000941:Antigens; D005349:Fibroins; D055767:Immobilized Proteins; D010446:Peptide Fragments; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase