title: Predicted peptides from non-structural proteins of porcine reproductive and respiratory syndrome virus are able to induce IFN-γ and IL-10.
abstract: This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-γ and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-γ responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide.  Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-γ responses on PHA-stimulated cells. Interestingly, the IFN-γ low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-γ responses as well as IL-10 inducing segments with inhibitory capabilities.
authors: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C (CIAD), Carretera a la Victoria Km 0.6, C.P. 83304 Hermosillo, Sonora, Mexico. alexel_25@hotmail.com
authors: Alexel
authors: A
authors: Burgara-Estrella
authors: Ivan
authors: I
authors: Díaz
authors: Irene M
authors: IM
authors: Rodríguez-Gómez
authors: Sabine E
authors: SE
authors: Essler
authors: Jesús
authors: J
authors: Hernández
authors: Enric
authors: E
authors: Mateu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism / Q000821:virology; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D017361:Viral Nonstructural Proteins; C438668:swine leukocyte antigen; D016753:Interleukin-10; D007371:Interferon-gamma