title: Sophoricoside isolated from Sophora japonica ameliorates contact dermatitis by inhibiting NF-κB signaling in B cells.
abstract: Sophoricoside (SOPH) is an isoflavone isolated from Sophora japonica (Leguminosae). In this study, the inhibitory effect of SOPH on contact dermatitis was investigated. At dosages of 3 and 10 mg/kg, SOPH ameliorated 2,4-dinitrochlorobenzene-induced acute and chronic contact dermatitis by 50-70%. As cellular targets, SOPH mainly affected the functions of B cells rather than T cells, macrophages and dendritic cells. As signaling targets, SOPH inhibited the phosphorylation and degradation of IκBα/β and the nuclear translocation of NF-κB p65 in B cells, but not in dendritic cells and macrophages. SOPH did not affect the phosphorylation of ERK, p38, and JNK MAPKs, in B cells, dendritic cells, and macrophages. Taken together, these results suggest that SOPH ameliorates contact dermatitis by inhibiting mainly NF-κB signaling in B cells.
authors: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
authors: Hong Kyung
authors: HK
authors: Lee
authors: Hyung Sook
authors: HS
authors: Kim
authors: Yeon Jin
authors: YJ
authors: Kim
authors: Ji Sung
authors: JS
authors: Kim
authors: Yoon Soo
authors: YS
authors: Park
authors: Jong Soon
authors: JS
authors: Kang
authors: Dong Yeon
authors: DY
authors: Yuk
authors: Jin Tae
authors: JT
authors: Hong
authors: Youngsoo
authors: Y
authors: Kim
authors: Sang-Bae
authors: SB
authors: Han
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D001578:Benzopyrans / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D003877:Dermatitis, Contact / Q000188:drug therapy* / Q000276:immunology; D004137:Dinitrochlorobenzene / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D029909:Sophora / Q000737:chemistry*
chemical_list: D001578:Benzopyrans; D004137:Dinitrochlorobenzene; D016328:NF-kappa B; C120642:sophoricoside