title: ASBEL, an ANA/BTG3 antisense transcript required for tumorigenicity of ovarian carcinoma.
abstract: Mammalian genomes encode numerous antisense non-coding RNAs, which are assumed to be involved in the regulation of the sense gene expression. However, the mechanisms of their action and involvement in the development of diseases have not been well elucidated. The ANA/BTG3 protein is an antiproliferative protein whose expression is downregulated in prostate and lung cancers. Here we show that an antisense transcript of the ANA/BTG3 gene, termed ASBEL, negatively regulates the levels of ANA/BTG3 protein, but not of ANA/BTG3 mRNA and is required for proliferation and tumorigenicity of ovarian clear cell carcinoma. We further show that knockdown of ANA/BTG3 rescues growth inhibition caused by ASBEL knockdown. Moreover, we demonstrate that ASBEL forms duplexes with ANA/BTG3 mRNA in the nucleus and suppresses its cytoplasmic transportation. Our findings illustrate a novel function for an antisense transcript that critically promotes tumorigenesis by suppressing translation of the sense gene by inhibiting its cytoplasmic transportation.
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors: Satoshi
authors: S
authors: Yanagida
authors: Kenzui
authors: K
authors: Taniue
authors: Hironobu
authors: H
authors: Sugimasa
authors: Emiko
authors: E
authors: Nasu
authors: Yasuko
authors: Y
authors: Takeda
authors: Mana
authors: M
authors: Kobayashi
authors: Tadashi
authors: T
authors: Yamamoto
authors: Aikou
authors: A
authors: Okamoto
authors: Tetsu
authors: T
authors: Akiyama
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001692:Biological Transport; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D023061:Gene Order; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D016372:RNA, Antisense; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C424522:BTG3 protein, human; D018797:Cell Cycle Proteins; D011506:Proteins; D016372:RNA, Antisense; D022661:RNA, Untranslated