title: Green tea extract supplementation induces the lipolytic pathway, attenuates obesity, and reduces low-grade inflammation in mice fed a high-fat diet.
abstract: The aim of this study was to evaluate the effects of green tea Camellia sinensis extract on proinflammatory molecules and lipolytic protein levels in adipose tissue of diet-induced obese mice. Animals were randomized into four groups: CW (chow diet and water); CG (chow diet and water + green tea extract); HW (high-fat diet and water); HG (high-fat diet and water + green tea extract). The mice were fed ad libitum with chow or high-fat diet and concomitantly supplemented (oral gavage) with 400 mg/kg body weight/day of green tea extract (CG and HG, resp.). The treatments were performed for eight weeks. UPLC showed that in 10 mg/mL green tea extract, there were 15 μg/mg epigallocatechin, 95 μg/mg epigallocatechin gallate, 20.8 μg/mg epicatechin gallate, and 4.9 μg/mg gallocatechin gallate. Green tea administered concomitantly with a high-fat diet increased HSL, ABHD5, and perilipin in mesenteric adipose tissue, and this was associated with reduced body weight and adipose tissue gain. Further, we observed that green tea supplementation reduced inflammatory cytokine TNFα levels, as well as TLR4, MYD88, and TRAF6 proinflammatory signalling. Our results show that green tea increases the lipolytic pathway and reduces adipose tissue, and this may explain the attenuation of low-grade inflammation in obese mice.
authors: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, Brazil.
authors: Cláudio A
authors: CA
authors: Cunha
authors: Fábio S
authors: FS
authors: Lira
authors: José C
authors: JC
authors: Rosa Neto
authors: Gustavo D
authors: GD
authors: Pimentel
authors: Gabriel I H
authors: GI
authors: Souza
authors: Camila Morais Gonçalves
authors: CM
authors: da Silva
authors: Cláudio T
authors: CT
authors: de Souza
authors: Eliane B
authors: EB
authors: Ribeiro
authors: Alexandra Christine Helena Frankland
authors: AC
authors: Sawaya
authors: Cláudia M
authors: CM
authors: Oller do Nascimento
authors: Bruno
authors: B
authors: Rodrigues
authors: Patrícia
authors: P
authors: de Oliveira Carvalho
authors: Lila M
authors: LM
authors: Oyama
mesh_terms: D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D059305:Diet, High-Fat / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D016753:Interleukin-10 / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy*; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism; D013662:Tea / Q000737:chemistry*; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D052242:Adiponectin; D053594:Myeloid Differentiation Factor 88; D048029:TNF Receptor-Associated Factor 6; D013662:Tea; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C417940:gallocatechin gallate; D016753:Interleukin-10; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate