title: [Microbiological characterization of non-O1 Vibrio cholerae isolated in Cuba].
abstract: The study of 422 non-01 Vibrio cholerae strains from nine provinces, 9 of them isolated from a water-borne disease outbreak, was performed. All the strains exhibited antimicrobial susceptibility and virulence factors. The nine strains from the outbreak were subjected to a DNA macrorestriction study based on the pulsed field electrophoresis technique. For the first time in Cuba and the Caribbean. The circulation of atypical non-01 V cholerae strains (resistent to vibriostatic compound 0129 and trimethoprim/sulfamethoxazole). The behavior of antimicrobial susceptibility evinced for the first time the circulation of two different resistence patterns in Cuba (ampicilline, trimethoprim/ sulfamethoxazole, sulfonamide and tetracycline, trimethoprim/ sulfamethoxazole, sulfonamide). The frequency of trimethoprim/ sulfamethoxazole-resistent strains was similar during the whole period of study. However, resistance to ampicilline decreased whereas resistance to tetracycline increased. The main found virulence factors were gelatinase, hemolysine, elastase and adherence to Hep-2 cells. On the other hand, the outbreak strains showed higher percentages than the others due to the presence of heat-liable toxin and fimbriae. The results of the molecular and epidemiological studies allowed giving a speedy and accurate response that explained the etiology of the first food-borne disease outbreak.
authors: Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba. laura@ipk.sld.cu
authors: Laura
authors: L
authors: Bravo Fariñas
authors: Anabel
authors: A
authors: Fernández
authors: María M
authors: MM
authors: Ramírez
authors: Alina
authors: A
authors: Llop
authors: Gerardo
authors: G
authors: Martínez
authors: Raquel I
authors: RI
authors: Hernández
authors: Luis E
authors: LE
authors: Cabrera
authors: Luis
authors: L
authors: Morier
authors: Jorge
authors: J
authors: Fraga
authors: Fidel A
authors: FA
authors: Núñez
authors: Adalberto
authors: A
authors: Aguila
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis; D002460:Cell Line; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology; D002772:Cholera Toxin / Q000032:analysis; D003462:Cuba / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D006801:Humans; D044163:Vibrio cholerae non-O1 / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D014774:Virulence; D014871:Water Microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002772:Cholera Toxin