title: The contribution of TNF-α in the amygdala to anxiety in mice with persistent inflammatory pain.
abstract: Tumor necrosis factor alpha (TNF-α) is known for its role in inflammation and pain, which are strongly associated with mood disorders such as anxiety and depression. The amygdala is a forebrain structure that modulates anxiety. However, little is known about the role of TNF-α in the development of anxiety in animals with chronic pain. In the present study, we examined TNF-α expression in the basolateral amygdala (BLA) following injection of complete Freund's adjuvant (CFA) into the hind paw of mice to induce inflammation. We also determined the effects of TNF-α expression on the development of anxiety in these mice. TNF-α expression was increased in the BLA during the chronic phase of CFA-induced peripheral inflammation. The local infusion of TNF-α-neutralizing antibody infliximab in the BLA reversed anxiety-like behaviors in mice with persistent inflammatory pain. In vitro slice recordings revealed that TNF-α significantly enhanced AMPA-receptor-mediated glutamatergic excitatory synaptic transmission and inhibited GABAA-receptor-mediated inhibitory synaptic transmission in the BLA. Our findings, therefore, provide strong evidence that TNF-α contributes to the development of anxiety in mice with persistent inflammatory pain.
authors: Institute of Osteosarcoma, Tangdu Hospital, Fourth Military Medical University, 1 Xinsi Road, Xi'an, Shaanxi 710038, China.
authors: Jun
authors: J
authors: Chen
authors: Yang
authors: Y
authors: Song
authors: Jing
authors: J
authors: Yang
authors: Yong
authors: Y
authors: Zhang
authors: Peng
authors: P
authors: Zhao
authors: Xue-Jun
authors: XJ
authors: Zhu
authors: Hai-Chuan
authors: HC
authors: Su
mesh_terms: D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D001007:Anxiety / Q000378:metabolism / Q000503:physiopathology / Q000523:psychology*; D059350:Chronic Pain / Q000139:chemically induced / Q000276:immunology / Q000523:psychology*; D005106:Exploratory Behavior; D005620:Freund's Adjuvant; D066298:In Vitro Techniques; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000503:physiopathology; D000069285:Infliximab; D008297:Male; D018782:Maze Learning; D051379:Mice; D008810:Mice, Inbred C57BL; D009435:Synaptic Transmission; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D014409:Tumor Necrosis Factor-alpha; D005620:Freund's Adjuvant; D000069285:Infliximab