title: High-performance surface acoustic wave immunosensing system on a PEG/aptamer hybridized surface.
abstract: Label-free immunoassay systems have the advantages of procedural simplicity and a low construction cost of surfaces for immunosensing. When label-free immunoassay systems are considered, the nonspecific adsorption of unwanted materials should be eliminated unless it aids in the detection of error. PEG is well-known as a blocking agent for the prevention of the adsorption of nonspecific binding materials when coimmobilized with ligands for targets such as antibodies and oligonucleotides. The construction strategy for PEG/ligand coimmobilized surfaces is an important point in the preparation of a high-performance assays because the physiological condition of the ligand depends strongly on its interaction with the PEG chain. In this report, we investigate the interaction between thrombin and a thrombin-binding aptamer (TBA) on a PEG/TBA coimmobilized surface by using a shear horizontal surface acoustic wave (SAW) sensor. The thrombin-TBA binding property shows remarkable differences with changes in the PEG density and the distance from the gold surface to the aptamer.
authors: Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Japan.
authors: Yukichi
authors: Y
authors: Horiguchi
authors: Seigo
authors: S
authors: Miyachi
authors: Yukio
authors: Y
authors: Nagasaki
mesh_terms: D000162:Acoustics; D000327:Adsorption; D001483:Base Sequence; D015374:Biosensing Techniques; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D011092:Polyethylene Glycols