title: Differential expression of benzophenone synthase and chalcone synthase in Hypericum sampsonii.
abstract: cDNAs encoding Hypericum sampsonii benzophenone synthase (HsBPS) and chalcone synthase (HsCHS) were isolated and functionally characterized. Differential expressions of HsBPS and HsCHS were monitored using quantitative polymerase chain reaction (PCR). In the vegetative stage, HsBPS was highly expressed in the roots; its transcript level was approx. 100 times higher than that of HsCHS. Relatively high transcript amounts of HsBPS were also detected in older leaves, whereas the youngest leaves contained higher transcript amounts of HsCHS. In the reproductive stage, maximum HsCHS expression was detected in flowers, the transcript level being approx. 5 times higher than that of HsBPS. The inversed situation with a 10-fold difference in the expression levels was observed with fruits. High transcript amounts for both proteins were found in roots.
authors: Key Laboratory of Plant Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China.
authors: Lili
authors: L
authors: Huang
authors: Hong
authors: H
authors: Wang
authors: Hechun
authors: H
authors: Ye
authors: Zhigao
authors: Z
authors: Du
authors: Yansheng
authors: Y
authors: Zhang
authors: Ludger
authors: L
authors: Beerhues
authors: Benye
authors: B
authors: Liu
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis* / Q000235:genetics; D019735:Carbon-Carbon Ligases / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000187:drug effects / Q000235:genetics; D035264:Flowers / Q000201:enzymology; D018506:Gene Expression Regulation, Plant; D020902:Hypericum / Q000201:enzymology* / Q000235:genetics*; D007700:Kinetics; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000201:enzymology; D016133:Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D000217:Acyltransferases; C020141:flavanone synthetase; D019735:Carbon-Carbon Ligases; C098887:benzophenone synthase