title: Effect of sinapic acid against dimethylnitrosamine-induced hepatic fibrosis in rats.
abstract: Sinapic acid is a member of the phenylpropanoid family and is abundant in cereals, nuts, oil seeds, and berries. It exhibits a wide range of pharmacological properties. In this study, we investigated the hepatoprotective and antifibrotic effects of sinapic acid on dimethylnitrosamine (DMN)-induced chronic liver injury in rats. Sinapic acid remarkably prevented DMN-induced loss of body weight. This was accompanied by a significant increase in levels of serum alanine transaminase, aspartate transaminase, and liver malondialdehyde content. Furthermore, sinapic acid reduced hepatic hydroxyproline content, which correlated with a reduction in the expression of type I collagen mRNA and histological analysis of collagen in liver tissue. Additionally, the expression of hepatic fibrosis-related factors such as α-smooth muscle actin and transforming growth factor-β1 (TGF-β1), were reduced in rats treated with sinapic acid. Sinapic acid exhibited strong scavenging activity. In conclusion, we find that sinapic acid exhibits hepatoprotective and antifibrotic effects against DMN-induced liver injury, most likely due to its antioxidant activities of scavenging radicals, its capacity to suppress TGF-β1 and its ability to attenuate activation of hepatic stellate cells. This suggests that sinapic acid is a potentially useful agent for the protection against liver fibrosis and cirrhosis.
authors: College of Pharmacy, Pusan National University, Busan, 609-735, Korea. takeastep@korea.kr
authors: Dong-Su
authors: DS
authors: Shin
authors: Kung Wook
authors: KW
authors: Kim
authors: Hae Young
authors: HY
authors: Chung
authors: Sik
authors: S
authors: Yoon
authors: Jeon-Ok
authors: JO
authors: Moon
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D015153:Blotting, Western; D003373:Coumaric Acids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004128:Dimethylnitrosamine / Q000633:toxicity*; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D008111:Liver Function Tests; D008297:Male; D015394:Molecular Structure; D010851:Picrates / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology
chemical_list: D001713:Biphenyl Compounds; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D010851:Picrates; D051996:Transcription Factor RelA; D053773:Transforming Growth Factor beta1; C073734:sinapinic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D004128:Dimethylnitrosamine