title: Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion.
abstract: Gene therapy is a very promising technology for treatment of liver diseases. Minicircle DNA (MCDNA) is a versatile gene vector, which possesses excellent features in bio-safety and duration of transgene expression. However, its application has been hampered by the lack of an efficient gene delivery system. In this study, we developed a magnetic resonance imaging (MRI) visible nanoparticle to monitor MCDNA gene delivery and explore the potential of gene therapy in vivo. The nanoparticle was constructed via self-assembly of stearic acid modified low molecular polyethyleneimine (stPEI) and superparamagnetic iron oxide (SPIO) nanocrystals. The multiple SPIO nanocrystals with a controlled clustering structure in the nanoparticles were designed to achieve high MRI sensitivity. Furthermore, the MCDNA was combined with the nanoparticles via electronic interaction. Through intrabiliary infusion, the stPEI-SPIO/MCDNA nanoparticles were efficiently delivered to liver that was visualized by MRI in vivo and confirmed by histology. Moreover, the MCDNA nanoparticles exhibited non-cytotoxicity with no obvious inflammation in the transfection sites. These results indicate that stPEI-SPIO nanoparticle is able to serve as both an efficient DNA vector delivery system and a sensitive agent for MRI visualization.
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, PR China.
authors: Lin
authors: L
authors: Gao
authors: Lisi
authors: L
authors: Xie
authors: Xiaojing
authors: X
authors: Long
authors: Zhiyong
authors: Z
authors: Wang
authors: Cheng-Yi
authors: CY
authors: He
authors: Zhi-Ying
authors: ZY
authors: Chen
authors: Lei
authors: L
authors: Zhang
authors: Xiang
authors: X
authors: Nan
authors: Hulong
authors: H
authors: Lei
authors: Xin
authors: X
authors: Liu
authors: Gang
authors: G
authors: Liu
authors: Jian
authors: J
authors: Lu
authors: Bensheng
authors: B
authors: Qiu
mesh_terms: D000818:Animals; D001659:Biliary Tract / Q000378:metabolism*; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry*; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D015316:Genetic Therapy; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D004247:DNA