title: A possible role for CD8+ and non-CD8+ cell granzyme B in early small airway wall remodelling in centrilobular emphysema.
abstract: OBJECTIVE
  CD8(+) cell infiltration and apoptosis of airway epithelial cells are increased in chronic obstructive pulmonary disease (COPD). CD8(+) T cells induce apoptosis by releasing granzymes, which can also cause extracellular matrix degradation and remodelling. Granzyme B levels and T cells expressing granzyme B are increased in bronchoalveolar lavage fluid of COPD patients, which suggests that granzyme B may contribute to the pathogenesis of COPD. This study provides quantitation of granzyme B-positive cells in relation to CD8(+) cells in the small airway walls of emphysema.
  
  
  METHODS
  Antibodies against CD8 and granzyme B were used to identify CD8(+) and granzyme B(+) cells. Volume fraction (Vv) of CD8(+) and granzyme B(+) cells were quantitated by the point counting method in the small airways of 13 non-smoker control subjects and 46 emphysema patients (14 panlobular emphysema (PLE) and 32 centrilobular emphysema (CLE) lungs). Immunohistochemical detection of macrophage scavenger receptor was also performed in randomly selected cases.
  
  
  RESULTS
  The Vv of CD8(+) and granzyme B(+) cells in CLE was greater than those in control and PLE (both P < 0.001) subjects. The Vv of granzyme B(+) cells was greater than that of CD8(+) cells (P = 0.006), and not all CD8(+) cells were positive for granzyme B in CLE subjects. Monocytes expressing both granzyme B and macrophage scavenger receptor and granulocytes expressing granzyme B were identified.
  
  
  CONCLUSIONS
  Upregulation of granzyme B in CD8(+) and non-CD8(+) cells is an early phenomenon of small airway wall remodelling in centrilobular emphysema and suggests its possible role in the pathogenesis of COPD.
authors: Division of Pulmonary Medicine, Konkuk University Medical Center, Seoul, Republic of Korea. wdkim@kuh.ac.kr
authors: Won-Dong
authors: WD
authors: Kim
authors: Hyun-Sook
authors: HS
authors: Chi
authors: Kang-Hyeon
authors: KH
authors: Choe
authors: Yeon-Mok
authors: YM
authors: Oh
authors: Sang-Do
authors: SD
authors: Lee
authors: Kyu-Rae
authors: KR
authors: Kim
authors: Kwang-Ha
authors: KH
authors: Yoo
authors: David A
authors: DA
authors: Ngan
authors: W Mark
authors: WM
authors: Elliott
authors: David J
authors: DJ
authors: Granville
authors: Don D
authors: DD
authors: Sin
authors: James C
authors: JC
authors: Hogg
mesh_terms: D000328:Adult; D000368:Aged; D056151:Airway Remodeling / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D053804:Granzymes / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology / Q000503:physiopathology; D011656:Pulmonary Emphysema / Q000378:metabolism / Q000503:physiopathology*; D020545:Respiratory Mucosa / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology
chemical_list: D053804:Granzymes