title: Recombinant streptavidin-C3bot for delivery of proteins into macrophages.
abstract: We demonstrated previously that monocytes and macrophages are target cells for the Rho-modifying Clostridium botulinum C3 ADP-ribosyltransferase. Here, we report the construction, expression and characterization of a recombinant streptavidin-C3 fusion protein which allows for delivery of biotin-labelled molecules into the cytosol of macrophages via enzymatically inactive C3bot1E174Q. The enzyme domain of diphtheria toxin was used as cargo to demonstrate proof of principle. This transport system could represent an attractive tool for experimental monocyte/macrophage pharmacology.
authors: Institute of Pharmacology and Toxicology, University of Ulm Medical Center, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
authors: Hannes
authors: H
authors: Christow
authors: Maren
authors: M
authors: Lillich
authors: Alexander
authors: A
authors: Sold
authors: Jörg
authors: J
authors: Fahrer
authors: Holger
authors: H
authors: Barth
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D001710:Biotin; D019921:Biotinylation; D001905:Botulinum Toxins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D016503:Drug Delivery Systems; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D019809:Streptavidin
chemical_list: D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins; D001710:Biotin; D019809:Streptavidin; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins
keywords: Biotin; Cellular transport; Clostridium botulinum C3 transferase; Fusion protein; Macrophages; Streptavidin