title: Use of neutral capillaries for the enantioseparation of N-benzoylated amino acids by capillary electrophoresis with bromobalhimycin as chiral selector.
abstract: In this study, the partial filling technique on both polycationic polymer hexadimethrine bromide (HDB) modified capillary and eCAP neutral capillary were systematically compared in order to enhance the enantioseparation ability of bromobalhimycin as CE additive. The separation conditions, such as pH, the plug length, and the concentration of bromobalhimycin, etc., were optimized in order to obtain satisfactory separations. As expected, for all tested 28 N-benzoylated amino acids, up to five times higher enantioresolutions were obtained on the eCAP neutral capillary compared to that on the polycationic polymer hexadimethrine bromide modified capillary. Moreover, 26 of 28 tested racemic compounds were almost baseline- resolved without observing any interference from the front of the plug of bromobalhimycin. Although the limitation of longer running time on the neutral capillary, it allows the use of higher content of bromobalhimycin in the running buffer without any interference on the detection of analytes when enantioseparations are more difficult to obtain.
authors: Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China.
authors: Yongbo
authors: Y
authors: Peng
authors: Tingting
authors: T
authors: Zhang
authors: Tingting
authors: T
authors: Wang
authors: Zhenghua
authors: Z
authors: Liu
authors: Jacques
authors: J
authors: Crommen
authors: Zhengjin
authors: Z
authors: Jiang
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006583:Hexadimethrine Bromide / Q000737:chemistry; D013237:Stereoisomerism; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D000596:Amino Acids; C584316:bromobalhimycin; D006583:Hexadimethrine Bromide; D014640:Vancomycin