title: Integrin αPS3/βν-mediated phagocytosis of apoptotic cells and bacteria in Drosophila.
abstract: Integrins exert a variety of cellular functions as heterodimers of two transmembrane subunits named α and β. Integrin βν, a β-subunit of Drosophila integrin, is involved in the phagocytosis of apoptotic cells and bacteria. Here, we searched for an α-subunit that forms a complex and cooperates with βν. Examinations of RNAi-treated animals suggested that αPS3, but not any of four other α-subunits, is required for the effective phagocytosis of apoptotic cells in Drosophila embryos. The mutation of αPS3-encoding scb, deficiency, insertion of P-element, or alteration of nucleotide sequences, brought about a reduction in the level of phagocytosis. The defect in phagocytosis by deficiency was reverted by the forced expression of scb. Furthermore, flies in which the expression of both αPS3 and βν was inhibited by RNAi showed a level of phagocytosis almost equal to that observed in flies with RNAi for either subunit alone. A loss of αPS3 also decreased the activity of larval hemocytes in the phagocytosis of Staphylococcus aureus. Finally, a co-immunoprecipitation analysis using a Drosophila cell line treated with a chemical cross-linker suggested a physical association between αPS3 and βν. These results collectively indicated that integrin αPS3/βν serves as a receptor in the phagocytosis of apoptotic cells and bacteria by Drosophila phagocytes.
authors: Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa 920-1192, Japan.
authors: Saori
authors: S
authors: Nonaka
authors: Kaz
authors: K
authors: Nagaosa
authors: Toshinobu
authors: T
authors: Mori
authors: Akiko
authors: A
authors: Shiratsuchi
authors: Yoshinobu
authors: Y
authors: Nakanishi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism*; D039001:Integrin alpha Chains / Q000235:genetics / Q000378:metabolism*; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010587:Phagocytosis / Q000502:physiology*; D013211:Staphylococcus aureus
chemical_list: D029721:Drosophila Proteins; D039001:Integrin alpha Chains; D039641:Integrin beta Chains; C572269:alpha PS3 integrin, Drosophila