title: Human papillomavirus detection from human immunodeficiency virus-infected Colombian women's paired urine and cervical samples.
abstract: Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance.
authors: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
authors: Marina
authors: M
authors: Munoz
authors: Milena
authors: M
authors: Camargo
authors: Sara C
authors: SC
authors: Soto-De Leon
authors: Ricardo
authors: R
authors: Sanchez
authors: Diana
authors: D
authors: Parra
authors: Andrea C
authors: AC
authors: Pineda
authors: Otto
authors: O
authors: Sussmann
authors: Antonio
authors: A
authors: Perez-Prados
authors: Manuel E
authors: ME
authors: Patarroyo
authors: Manuel A
authors: MA
authors: Patarroyo
mesh_terms: D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000821:virology*; D060085:Coinfection / Q000175:diagnosis / Q000652:urine / Q000821:virology; D003105:Colombia; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000175:diagnosis / Q000652:urine / Q000821:virology*; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D058952:Human papillomavirus 31 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000652:urine / Q000821:virology*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D002583:Uterine Cervical Neoplasms / Q000517:prevention & control / Q000652:urine / Q000821:virology; D014626:Vaginal Smears; D055815:Young Adult
chemical_list: D004279:DNA, Viral