title: Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells.
abstract: Ferredoxin 1 (FDX1; adrenodoxin) is an iron-sulfur protein that is involved in various metabolic processes, including steroid hormone synthesis in mammalian tissues. We investigated the transcriptional regulation of FDX1 in ovarian granulosa cells. Previously, we reported that the NR5A family, including steroidogenic factor-1 (SF-1) and liver receptor homolog-1 could induce differentiation of human mesenchymal stem cells (hMSCs) into steroidogenic cells. A ChIP assay showed that SF-1 could bind to the FDX1 promoter in differentiated hMSCs. Luciferase reporter assays showed that transcription of FDX1 was synergistically activated by the NR5A family and 8Br-cAMP treatment through two SF-1 binding sites and a CRE-like sequence in a human ovarian granulosa cell line, KGN. Knockdown of FDX1 attenuated progesterone production in KGN cells. These results indicate transcription of FDX1 is regulated by the NR5A family and cAMP signaling, and participates in steroid hormone production in ovarian granulosa cells.
authors: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
authors: Yoshitaka
authors: Y
authors: Imamichi
authors: Tetsuya
authors: T
authors: Mizutani
authors: Yunfeng
authors: Y
authors: Ju
authors: Takehiro
authors: T
authors: Matsumura
authors: Shinya
authors: S
authors: Kawabe
authors: Masafumi
authors: M
authors: Kanno
authors: Takashi
authors: T
authors: Yazawa
authors: Kaoru
authors: K
authors: Miyamoto
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000325:Adrenodoxin / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D000242:Cyclic AMP / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D005288:Ferredoxins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D015398:Signal Transduction; D054339:Steroidogenic Factor 1 / Q000187:drug effects / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005288:Ferredoxins; C417002:NR5A2 protein, rat; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D000325:Adrenodoxin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011374:Progesterone; D000242:Cyclic AMP