title: Anti-mycobacterial activity of marine fungus-derived 4-deoxybostrycin and nigrosporin.
abstract: 4-Deoxybostrycin is a natural anthraquinone compound isolated from the Mangrove endophytic fungus Nigrospora sp. collected from the South China Sea. Nigrosporin is the deoxy-derivative of 4-deoxybostrycin. They were tested against mycobacteria, especially Mycobacterium tuberculosis. In the Kirby-Bauer disk diffusion susceptibility test, they both had inhibition zone sizes of over 25 mm. The results of the absolute concentration susceptibility test suggested that they had inhibitory effects against mycobacteria. Moreover, 4-deoxybostrycin exhibited good inhibition which was even better than that of first  line anti-tuberculosis (TB) drugs against some clinical multidrug-resistant (MDR)  M. tuberculosis strains. The gene expression profile of M. tuberculosis H37Rv after treatment with 4-deoxybostrycin was compared with untreated bacteria. One hundred and nineteen out of 3,875 genes were significantly different in M. tuberculosis exposed to 4-deoxybostrycin from control. There were 46 functionally known genes which are involved in metabolism, information storage and processing and cellular processes. The differential expressions of six genes were further confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). The present study provides a useful experiment basis for exploitation of correlative new drugs against TB and for finding out new targets of anti-mycobacterial therapy.
authors: Department of Microbiology, Gangdong Provincial Research Centre for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong, China.
authors: Cong
authors: C
authors: Wang
authors: Juan
authors: J
authors: Wang
authors: Yuhong
authors: Y
authors: Huang
authors: Hong
authors: H
authors: Chen
authors: Yan
authors: Y
authors: Li
authors: Lili
authors: L
authors: Zhong
authors: Yi
authors: Y
authors: Chen
authors: Shengping
authors: S
authors: Chen
authors: Jun
authors: J
authors: Wang
authors: Juling
authors: J
authors: Kang
authors: Yi
authors: Y
authors: Peng
authors: Bin
authors: B
authors: Yang
authors: Yongcheng
authors: Y
authors: Lin
authors: Zhigang
authors: Z
authors: She
authors: Xiaomin
authors: X
authors: Lai
mesh_terms: D000880:Anthraquinones / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D005658:Fungi / Q000737:chemistry*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012623:Seawater / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D000995:Antitubercular Agents; C582135:nigrosporin; C034288:4-deoxybostrycin