title: Microbial community signature of high-solid content methanogenic ecosystems.
abstract: In this study, changes in bacterial and archaeal communities involved in anaerobic digestion processes operated with high solid contents were investigated. Batch tests were performed within a range of total solids (TS) of 10-35%. Between 10% and 25% TS, high methanogenic activity was observed and no overall specific structure of active bacterial communities was found. At 30% and 35%, methanogenesis was inhibited as a consequence of volatile fatty acids accumulation. Here, a specific bacterial signature was observed with three main dominant bacteria related to Clostridium sp., known for their ability to grow at low pH. Additionally, archaeal community was gradually impacted by TS content. Three archaeal community structures were observed with a gradual shift from Methanobacterium sp. to Methanosarcina sp., according to the TS content. Overall, several species were identified as biomarkers of methanogenesis inhibition, since bacterial and archaeal communities were highly specific at high TS contents.
authors: INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France.
authors: Amel
authors: A
authors: Abbassi-Guendouz
authors: Eric
authors: E
authors: Trably
authors: Jérôme
authors: J
authors: Hamelin
authors: Claire
authors: C
authors: Dumas
authors: Jean Philippe
authors: JP
authors: Steyer
authors: Jean-Philippe
authors: JP
authors: Delgenès
authors: Renaud
authors: R
authors: Escudié
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D016172:DNA Fingerprinting; D017753:Ecosystem; D019075:Electrophoresis, Capillary; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D008697:Methane