title: [Effects of carbon disulfide exposure during peri-implantation on estrogen receptor-α expression in uterus and serum level of estrogen in pregnant mice].
abstract: OBJECTIVE
  To evaluate the effects of carbon disulfide (CS(2)) exposure during peri-implantation on the estrogen receptor-α (ER-α) expression in the uterus and serum level of estradiol (E(2)) in pregnant mice, and to explore the mechanism of embryotoxicity of CS(2).
  
  
  METHODS
  Healthy female mice were exposed to a single dose of CS(2) (631.4 mg/kg) or olive oil (solvent control) on gestational day (GD)3, GD4, GD5, or GD6. At different time points after exposure, the serum E(2) levels of the pregnant mice were measured by enzyme-linked immunosorbent assay, and the expression levels of ER-α in the uterus were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot.
  
  
  RESULTS
  Compared with the control group, the GD3, GD4, GD5, and GD6 exposure groups showed significantly decreased serum E(2) levels on day 7 of gestation (P < 0.05); the GD3 and GD5 exposure groups showed significantly decreased serum E(2) levels on day 6 of gestation (P < 0.05). The expression level of ER-α in the GD 4 exposure group was 23.6% lower than that in the control group on day 5 of gestation, and the expression level of ER-α in the GD 5 exposure group was 72.9% lower than that in the control group on day 6 of gestation (P < 0.05); the GD 3 and GD 6 exposure groups showed lower expression levels of ER-α than the control group at any time point, but no significant difference was found (P > 0.05).
  
  
  CONCLUSIONS
  CS(2) exposure during peri-implantation can reduce the ER-α expression in the uterus and the serum level of E(2) in pregnant mice, which may be one of the mechanisms of embryotoxicity of CS(2).
authors: Institute of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China.
authors: Bing-zhen
authors: BZ
authors: Zhang
authors: Yan-ling
authors: YL
authors: Wu
authors: Bing-qin
authors: BQ
authors: Dai
authors: Chun-hui
authors: CH
authors: Li
authors: Liu
authors: L
authors: Yang
authors: Zhi-ping
authors: ZP
authors: Wang
mesh_terms: D000818:Animals; D002246:Carbon Disulfide / Q000633:toxicity*; D010064:Embryo Implantation; D004958:Estradiol / Q000097:blood*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; D004958:Estradiol; D002246:Carbon Disulfide