title: Recombinant Salmonella-based 4-1BBL vaccine enhances T cell immunity and inhibits the development of colorectal cancer in rats: in vivo effects of vaccine containing 4-1BBL.
abstract: BACKGROUND
  Immunotherapy with vaccines is attractive for the treatment of cancer. This study is aimed at determining the effect of recombinant Salmonella (SL3261)-based 4-1BB ligand (4-1BBL) vaccine on the development of colorectal cancers and the potential immune mechanisms in rats.
  
  
  RESULTS
  In comparison with that in the PBS group, similar levels of 4-1BBL expression, the frequency of T cells, IFN-γ responses, and comparable numbers of tumors were detected in the SL3261 and SL3261C groups of rats. In contrast, significantly fewer numbers of tumors, increased levels of 4-1BBL expression in the spleens and colorectal tissues, higher frequency of peripheral blood and splenic CD3+CD25+ T cells, and stronger splenic T cell IFN-γ responses were detected in the SL3261R group of rats.
  
  
  CONCLUSIONS
  Our results indicated that vaccination with recombinant attenuated Salmonella harboring the 4-1BBL gene efficiently enhanced T cell immunity and inhibited the development of carcinogen-induced colorectal cancers in rats.
authors: Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Jianxin
authors: J
authors: Ye
authors: Ling
authors: L
authors: Li
authors: Yuanting
authors: Y
authors: Zhang
authors: Xueguang
authors: X
authors: Zhang
authors: Daming
authors: D
authors: Ren
authors: Weichang
authors: W
authors: Chen
mesh_terms: D053330:4-1BB Ligand / Q000276:immunology*; D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D051381:Rats; D022562:Salmonella Vaccines / Q000008:administration & dosage*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage
chemical_list: D053330:4-1BB Ligand; D019496:Cancer Vaccines; D022562:Salmonella Vaccines; D014614:Vaccines, Synthetic