title: Uropathogenic Escherichia coli causes cortical tubular necrotic cell death and the release of macrophage migration inhibitory factor.
abstract: The macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, is deregulated in acute kidney injury (AKI) through an unknown mechanism. In the present study, we used a previously described mouse model of ascending urinary tract infection in which uropathogenic Escherichia coli (UPEC) were transurethrally inoculated to induce kidney infections. Here, we show that urinary MIF was upregulated during AKI while MIF was abundantly expressed in the renal cortical tubules and that UPEC infection caused a decrease in tubular MIF. Infections with UPEC in vitro caused MIF release in a cell type-dependent manner, which was independent of receptor-mediated internalization, signal transduction, and transcription. Indeed, UPEC infection-induced necrotic cell death in vitro and in vivo correlated with extracellular acidification and processed MIF secretion. These data suggest that MIF is released by necrotic renal cortical tubular cells during UPEC infection.
authors: Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
authors: Ming-Yuan
authors: MY
authors: Hong
authors: Chin-Chung
authors: CC
authors: Tseng
authors: Chia-Chang
authors: CC
authors: Chuang
authors: Chia-Ling
authors: CL
authors: Chen
authors: Yu-Huei
authors: YH
authors: Lin
authors: Chia-Yuan
authors: CY
authors: Hsieh
authors: Yu-Tzu
authors: YT
authors: Chang
authors: Chung-Hsi
authors: CH
authors: Hsing
authors: Kwang-Yu
authors: KY
authors: Chang
authors: Chiou-Feng
authors: CF
authors: Lin
mesh_terms: D000143:Acids / Q000378:metabolism; D058186:Acute Kidney Injury / Q000382:microbiology / Q000473:pathology / Q000652:urine; D000818:Animals; D016923:Cell Death; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology* / Q000652:urine; D005260:Female; D006801:Humans; D007672:Kidney Cortex / Q000382:microbiology / Q000473:pathology* / Q000648:ultrastructure; D007684:Kidney Tubules / Q000382:microbiology / Q000473:pathology* / Q000648:ultrastructure; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism* / Q000652:urine; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis; D009928:Organ Specificity; D015398:Signal Transduction; D014158:Transcription, Genetic; D014552:Urinary Tract Infections / Q000382:microbiology / Q000473:pathology* / Q000652:urine; D056689:Uropathogenic Escherichia coli / Q000502:physiology*
chemical_list: D000143:Acids; D008263:Macrophage Migration-Inhibitory Factors