title: The expression and clinical significance of microRNA-1258 and heparanase in human breast cancer.
abstract: OBJECTIVE
  To investigate the expression profile of miR-1258 and heparanase (HPSE) in breast cancer and to assess their clinicopathological significance.
  
  
  METHODS
  The expression levels of miR-1258 and HPSE were analyzed in normal, benign and malignant breast tissues. Their serum levels were evaluated in healthy women and in patients with benign and malignant breast disease. We studied the correlation between the expression of miR-1258 and HPSE and the clinical features presented by the patients.
  
  
  RESULTS
  MiR-1258 was down-regulated and HPSE was up-regulated in breast cancer, with a significant inverse correlation. A reduced miR-1258 expression and an elevated HPSE expression were associated with the lymph node status, late clinical stages, a short overall survival and a short relapse-free survival. In frozen fresh tissue samples, the miR-1258 levels in breast cancer with lymph node metastasis were significantly lower than that of breast cancer without lymph node metastasis and benign disease (BD). In contrast, the HPSE levels in breast cancer with lymph node metastasis were the highest. In serum samples, the miR-1258 levels in metastatic breast cancer (M1) were lower than that of primary breast cancer (M0) and BD. However, serum HPSE levels of M1 patients were significantly higher than that of M0 patients and BD patients.
  
  
  CONCLUSIONS
  MiR-1258 may play an important role in breast cancer development and progression by regulating the expression of HPSE, and they might be potential prognostic biomarkers for breast cancer.
authors: Department of Medical Oncology, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, China. doctortang2008@163.com
authors: Dabei
authors: D
authors: Tang
authors: Qingyuan
authors: Q
authors: Zhang
authors: Shu
authors: S
authors: Zhao
authors: Jincai
authors: J
authors: Wang
authors: Kangping
authors: K
authors: Lu
authors: Ying
authors: Y
authors: Song
authors: Ling
authors: L
authors: Zhao
authors: Xinmei
authors: X
authors: Kang
authors: Jingxuan
authors: J
authors: Wang
authors: Shanqi
authors: S
authors: Xu
authors: Lantian
authors: L
authors: Tian
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005966:Glucuronidase / Q000096:biosynthesis*; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D015996:Survival Rate; D000099175:Heparanase
chemical_list: D014408:Biomarkers, Tumor; D005966:Glucuronidase; D035683:MicroRNAs; D012334:RNA, Neoplasm; D000099175:Heparanase; C576996:MIRN1258 microRNA, human