title: Reference ranges for lymphocyte subsets among healthy Hong Kong Chinese adults by single-platform flow cytometry.
abstract: Race, age, sex, and environmental conditions have significant impacts on lymphocyte subset values. It is important to establish the local reference ranges from healthy and non-HIV-positive adults in the local population for clinical decision making. In this study, the reference ranges for lymphocyte subsets among Chinese adults were established by analysis by single-platform flow cytometry of the lymphocyte compositions of 273 healthy adult blood donors between 17 and 59 years of age. The 95% reference ranges for CD3(+) T cells, CD3(+) CD4(+) T helper cells, and CD3(+) CD8(+) T suppressor cells are 723 to 2,271 cells/μl, 396 to 1,309 cells/μl, and 224 to 1,014 cells/μl, respectively. The 95% reference ranges for CD19(+) B cells and CD56(+) NK cells are 118 to 645 cells/μl and 61 to 607 cells/μl, respectively. Significant gender and age differences in the lymphocyte subsets have been demonstrated. Our results have also shown that the T-lymphocyte compositions in Hong Kong Chinese were comparable to those of other Asian populations but were different from those of Caucasians.
authors: Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, China. sws_wong@yahoo.com.hk
authors: W S
authors: WS
authors: Wong
authors: A W I
authors: AW
authors: Lo
authors: L P
authors: LP
authors: Siu
authors: J N S
authors: JN
authors: Leung
authors: S P
authors: SP
authors: Tu
authors: S W
authors: SW
authors: Tai
authors: S C
authors: SC
authors: Lam
authors: K F
authors: KF
authors: Wong
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D044466:Asian People; D001782:Blood Donors; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006723:Hong Kong; D006801:Humans; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012737:Sex Factors; D055815:Young Adult