title: Surface initiated polymerization of a cationic monomer on inner surfaces of silica capillaries: analyte separation by capillary electrophoresis versus polyelectrolyte behavior.
abstract: [2-(Methacryloyl)oxyethyl]trimethylammonium chloride was successfully polymerized by surface-initiated atom transfer radical polymerization method on the inner surface of fused-silica capillaries resulting in a covalently bound poly([2-(methacryloyl)oxyethyl]trimethylammonium chloride) coating. The coated capillaries provided in capillary electrophoresis an excellent run-to-run repeatability, capillary-to-capillary and day-to-day reproducibility. The capillaries worked reliably over 1 month with EOF repeatability below 0.5%. The positively charged coated capillaries were successfully applied to the capillary electrophoretic separation of three standard proteins and five β-blockers with the separation efficiencies ranging from 132,000 to 303,000 plates/m, and from 82,000 to 189,000 plates/m, respectively. In addition, challenging high- and low-density lipoprotein particles could be separated. The hydrodynamic sizes of free polymer chains in buffers used in the capillary electrophoretic experiments were measured for the characterization of the coatings.
authors: Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland.
authors: Joanna
authors: J
authors: Witos
authors: Mikko
authors: M
authors: Karesoja
authors: Erno
authors: E
authors: Karjalainen
authors: Heikki
authors: H
authors: Tenhu
authors: Marja-Liisa
authors: ML
authors: Riekkola
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D002412:Cations / Q000737:chemistry*; D002645:Chickens; D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006736:Horses; D006801:Humans; D058105:Polymerization; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*; D013552:Swine
chemical_list: D000319:Adrenergic beta-Antagonists; D002412:Cations; D004573:Electrolytes; D011506:Proteins; D012822:Silicon Dioxide