title: Staphylococcus aureus proteins differentially produced in ewe gangrenous mastitis or ewe milk.
abstract: Despite being one of the main pathogens involved in ruminant mastitis, little is known about what proteins Staphylococcus aureus does express, in vivo, during the infection. Here, two S. aureus strains were isolated from curds formed within the udder of two ewes suffering from gangrenous mastitis. Protein samples were prepared from cell fractions and were analyzed using 1D-LC MS/MS. Results were compared to 1D-LC MS/MS analysis of the same S. aureus strains grown in ewe milk. A total of 365 proteins were identified. Most of them were related to cellular metabolism, cellular division and stress response. Half of the proteins were found in both conditions but a substantial number were specifically found in in vivo conditions and gave indications about the active metabolic status and the stresses encountered by S. aureus within the cistern during a gangrenous mastitis.
authors: INRA, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France.
authors: Caroline
authors: C
authors: Le Maréchal
authors: Julien
authors: J
authors: Jardin
authors: Valérie
authors: V
authors: Briard-Bion
authors: Lucie
authors: L
authors: Rault
authors: Nadia
authors: N
authors: Berkova
authors: Eric
authors: E
authors: Vautor
authors: Richard
authors: R
authors: Thiéry
authors: Sergine
authors: S
authors: Even
authors: Yves
authors: Y
authors: Le Loir
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D005260:Female; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary*; D008892:Milk / Q000382:microbiology; D020543:Proteome / Q000032:analysis; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification*; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome