title: Hwangdonia seohaensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a tidal flat sediment.
abstract: A Gram-staining-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, HD-3(T), was isolated from a tidal flat sediment of Hwangdo in the Yellow Sea, South Korea. Strain HD-3(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain HD-3(T) joined the cluster comprising the type strains of Jejuia pallidilutea and Hyunsoonleella jejuensis. Strain HD-3(T) exhibited 16S rRNA gene sequence similarity values of 93.8% to each of the type strains of J. pallidilutea and Hyunsoonleella jejuensis. Strain HD-3(T) contained MK-6 as the only menaquinone and iso-C(15:0), iso-C(15:1) G, iso-C(17:0) 3-OH and iso-C(15:0) 3-OH as the major fatty acids. The polar lipid profile of strain HD-3(T), which contained phosphatidylethanolamine and one unidentified lipid as major components and one unidentified phospholipid as a significant component, differed slightly from those of the type strains of J. pallidilutea and Hyunsoonleella jejuensis. The DNA G+C content of strain HD-3(T) was 42.3 mol%. Differential phenotypic and chemotaxonomic properties and phylogenetic data of strain HD-3(T) demonstrated that this strain is distinguishable from J. pallidilutea and Hyunsoonleella jejuensis. On the basis of the data presented, strain HD-3(T) is considered to represent a novel genus and species, for which the name Hwangdonia seohaensis gen. nov., sp. nov. is proposed. The type strain is HD-3(T) ( =KCTC 32177(T) =CCUG 63246(T)).
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea.
authors: Yong-Taek
authors: YT
authors: Jung
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.|University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea.
authors: Jung-Sook
authors: JS
authors: Lee
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6