title: Effects of adenosine extract from Pholiota adiposa (Fr.) quel on mRNA expressions of superoxide dismutase and immunomodulatory cytokines.
abstract: Pholiota adiposa is a kind of edible mushroom which has long been known for its health care applications. To reveal the exact mechanism of its protective functions in humans, in this study we isolated and identified the active compound PB3 of P. adiposa for the first time by a combination of chromatography techniques, including NKA macroporous resin and Sephadex G-15. PB3, with molecular mass of 267.2 Da and molecular formula of C₁₀H₁₃N₅O₄ discovered by mass spectrum (MS) was identified to be adenosine. Mice were injected intraperitoneally with purified fraction PB3. Seven days after injection, we found a 1.5-fold increase of IL10 at the mRNA level, while a down regulated expression of IL-2, IL-6 and IFN-γ to 49.0%, 56.9% and 73.4%, respectively, was detected in spleen by real-time quantitative PCR. What's more, SOD expression level was significantly increased by 1.6-fold compared to control. Fraction PB3 displayed anti-inflammatory potency and heightened SOD activity on the transcriptional level, which could be considered of further pharmaceutical or medication value.
authors: Department of Microbiology, College of Life Science, Nankai University, Tianjin 300071, China.
authors: Chang Rong
authors: CR
authors: Wang
authors: Wen Tao
authors: WT
authors: Qiao
authors: Ye Ni
authors: YN
authors: Zhang
authors: Fang
authors: F
authors: Liu
mesh_terms: D000241:Adenosine / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D007155:Immunologic Factors / Q000235:genetics* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D055368:Pholiota / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000241:Adenosine