title: Recombinant production of proteoglycans and their bioactive domains.
abstract: Proteoglycans are ubiquitous dynamic molecules that are made up of a protein core to which specific linear glycosylation structures, known as glycosaminoglycans, have been covalently coupled. They have roles in many biological and pathological processes, which have been shown to be dependent on events involving the protein component and/or the glycosaminoglycan chains. This review focuses on the literature describing the recombinant expression and production of proteoglycans known to be present in the extracellular, cell surface and intracellular environments with an emphasis on how the structure of the molecule relates to its biological function and how this relationship has been explored using recombinant DNA technology for clinical applications.
authors: Graduate School of Biomedical Engineering, The University of New South Wales, Sydney, NSW, Australia.
authors: Megan S
authors: MS
authors: Lord
authors: John M
authors: JM
authors: Whitelock
mesh_terms: D053674:Aggrecans / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D058578:Biglycan / Q000235:genetics / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism; D058575:Decorin / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D053667:Syndecans / Q000235:genetics / Q000378:metabolism*; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D053674:Aggrecans; D058578:Biglycan; D011508:Chondroitin Sulfate Proteoglycans; D058575:Decorin; D016023:Integrins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D053667:Syndecans; C508319:ACAN protein, human; C546884:BGN protein, human; C101390:CSPG4 protein, human; D000099216:Chondroitin Sulfate Proteoglycan 4; C546878:DCN protein, human