title: Oligomeric procyanidins of lotus seedpod inhibits the formation of advanced glycation end-products by scavenging reactive carbonyls.
abstract: It has been reported that oligomeric procyanidins of lotus seedpod (LSOPC) is effective in the alleviation of Alzheimer's disease and diabetes through its antioxidant and insulin-potentiating activities. This study investigated the anti-glycative activity of LSOPC in a bovine serum albumin (BSA)-glucose model. The level of glycation and conformational alterations were assessed by specific fluorescence, Congo red binding assay and circular dichroism. The results show that LSOPC has a significant anti-glycative activity in vitro and it can also effectively protect the secondary structure of BSA during glycation. LSOPC or catechin (a major constituent unit of LSOPC), were used to react with methylglyoxal. The structures of their carbonyl adducts were tentatively identified using HPLC-MS(2). Their capacity to scavenge methylglyoxal suggested carbonyl scavenging as a major mechanism of antiglycation. Therefore, LSOPC could be helpful to prevent AGEs-associated diseases, and with the potential to be used as functional food ingredients.
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China. qianwill2007@163.com
authors: Qian
authors: Q
authors: Wu
authors: Hengye
authors: H
authors: Chen
authors: Zhejuan
authors: Z
authors: Lv
authors: Shuyi
authors: S
authors: Li
authors: Bei
authors: B
authors: Hu
authors: Yafei
authors: Y
authors: Guan
authors: Bijun
authors: B
authors: Xie
authors: Zhida
authors: Z
authors: Sun
mesh_terms: D016166:Free Radical Scavengers / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors / Q000737:chemistry*; D000070116:Lotus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D044945:Proanthocyanidins