title: Comparing the diagnostic values of circulating microRNAs and cardiac troponin T in patients with acute myocardial infarction.
abstract: OBJECTIVE
  Recent studies have shown that circulating microRNAs might be useful, novel biomarkers for the diagnosis of acute myocardial infarction. The aims of this study were to evaluate the expression of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) in patients with acute myocardial infarction and to compare the diagnostic values of these miRNAs with that of cardiac troponin T.
  
  
  METHODS
  Sixty-seven plasma samples obtained from patients with acute myocardial infarction and 32 plasma specimens collected from healthy volunteers were analyzed in this study. The levels of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) were measured by quantitative reverse transcription-polymerase chain reaction, and the concentrations of plasma cardiac troponin T were measured using electrochemiluminescence-based methods and an Elecsys 2010 Immunoassay Analyzer.
  
  
  RESULTS
  The levels of plasma miR-1, -133a, -208b, and -499 were significantly higher in acute myocardial infarction patients (all p<0.001) than in healthy volunteers. The expression of the cardiac-specific miRNAs in acute myocardial infarction patients decreased to close to the baseline levels at the time of hospital discharge (all p>0.05). There were no correlations between the levels of the four circulating miRNAs and the clinical characteristics of the study population (all p>0.05). Furthermore, receiver operating characteristic curve analyses showed that the four plasma miRNAs were not superior to cardiac troponin T for the diagnosis of acute myocardial infarction (all p>0.05).
  
  
  CONCLUSIONS
  Our results demonstrate that circulating miR-1, -133a, -208b, and -499 may be useful biomarkers in acute myocardial infarction patients but that these miRNAs are not superior to cardiac troponin T for the diagnosis of acute myocardial infarction.
authors: Sun Yat-Sen University, The First Affiliated Hospital, Department of Emergency, Guangzhou, People's Republic of China.
authors: Ying-Qing
authors: YQ
authors: Li
authors: Mei-Fen
authors: MF
authors: Zhang
authors: Hong-Yan
authors: HY
authors: Wen
authors: Chun-Lin
authors: CL
authors: Hu
authors: Rong
authors: R
authors: Liu
authors: Hong-Yan
authors: HY
authors: Wei
authors: Chen-Mu
authors: CM
authors: Ai
authors: Gang
authors: G
authors: Wang
authors: Xiao-Xing
authors: XX
authors: Liao
authors: Xin
authors: X
authors: Li
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D004812:Epidemiologic Methods; D005260:Female; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000235:genetics; D011237:Predictive Value of Tests; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020107:Troponin T / Q000097:blood*
chemical_list: D015415:Biomarkers; C548234:MIRN133 microRNA, human; C550906:MIRN208 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs; D020107:Troponin T