title: Localization and functional properties of two galectin-1 proteins in Atlantic cod (Gadus morhua) mucosal tissues.
abstract: Galectin-1 is a β-galactoside binding lectin with multiple immune functions in higher vertebrates. We report the characterization of two galectin-1 proteins from Atlantic cod, with emphasis on mucosal tissues. Tissue distribution of these two ≈14kDa galectin-1 proteins (Codgal1-1 and Codgal1-2) was ascertained by western blotting of one dimensional (1D) and two dimensional (2DE) gels. The two galectin-1 proteins were differentially localized in the mucosal tissues of cod. Codgal1-1 was predominantly localized in the basal cells of skin and this protein was present in all the early developmental stages examined, indicating a likely involvement in developmental processes. The two lectins were also localized in the adherent macrophage-like cells (MLC) from cod head kidney and results gathered indicate their possible secretion during Francisella noatunensis infection, suggesting that they are active components of immune defence. Lactose affinity chromatography coupled with gel filtration co-purified the two cod galectin-1 proteins, which hemagglutinated horse red blood cells in a lactose inhibitable manner. They also could bind and agglutinate both Gram-positive and Gram-negative bacteria. This study suggests multiple functional roles for galectin-1, especially in development and innate immune response of Atlantic cod.
authors: Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø, Norway.
authors: Binoy
authors: B
authors: Rajan
authors: Viswanath
authors: V
authors: Kiron
authors: Jorge M O
authors: JM
authors: Fernandes
authors: Monica F
authors: MF
authors: Brinchmann
mesh_terms: D048409:Aeromonas salmonicida / Q000187:drug effects; D000371:Agglutination; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D048389:Gadus morhua / Q000254:growth & development / Q000378:metabolism*; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D008264:Macrophages / Q000378:metabolism; D016982:Micrococcus luteus / Q000187:drug effects; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000378:metabolism; D009928:Organ Specificity; D058642:Planococcus Bacteria / Q000187:drug effects; D012867:Skin / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D037483:Galectin 1