title: The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression.
abstract: Interactions between phytohormones play important roles in the regulation of plant growth and development, but knowledge of the networks controlling hormonal relationships, such as between oxylipins and auxins, is just emerging. Here, we report the transcriptional regulation of two Arabidopsis YUCCA genes, YUC8 and YUC9, by oxylipins. Similar to previously characterized YUCCA family members, we show that both YUC8 and YUC9 are involved in auxin biosynthesis, as demonstrated by the increased auxin contents and auxin-dependent phenotypes displayed by gain-of-function mutants as well as the significantly decreased indole-3-acetic acid (IAA) levels in yuc8 and yuc8/9 knockout lines. Gene expression data obtained by qPCR analysis and microscopic examination of promoter-reporter lines reveal an oxylipin-mediated regulation of YUC9 expression that is dependent on the COI1 signal transduction pathway. In support of these findings, the roots of the analyzed yuc knockout mutants displayed a reduced response to methyl jasmonate (MeJA). The similar response of the yuc8 and yuc9 mutants to MeJA in cotyledons and hypocotyls suggests functional overlap of YUC8 and YUC9 in aerial tissues, while their function in roots shows some specificity, probably in part related to different spatio-temporal expression patterns of the two genes. These results provide evidence for an intimate functional relationship between oxylipin signaling and auxin homeostasis.
authors: Department of Plant Physiology, Ruhr-University Bochum, Universitätsstraβe 150, 44780, Bochum, Germany.
authors: Mathias
authors: M
authors: Hentrich
authors: Christine
authors: C
authors: Böttcher
authors: Petra
authors: P
authors: Düchting
authors: Youfa
authors: Y
authors: Cheng
authors: Yunde
authors: Y
authors: Zhao
authors: Oliver
authors: O
authors: Berkowitz
authors: Josette
authors: J
authors: Masle
authors: Joaquín
authors: J
authors: Medina
authors: Stephan
authors: S
authors: Pollmann
mesh_terms: D000085:Acetates / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018548:Cotyledon / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D006706:Homeostasis; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010641:Phenotype; D035261:Plant Components, Aerial / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D030821:Plants, Genetically Modified; D015398:Signal Transduction
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; C030737:indoleacetic acid; C072239:methyl jasmonate; D006899:Mixed Function Oxygenases; C585335:YUCCA8 protein, Arabidopsis; C585336:YUCCA9 protein, Arabidopsis; D010105:Oxygenases; C422746:YUC protein, Arabidopsis