title: Biomimetic enzyme nanocomplexes and their use as antidotes and preventive measures for alcohol intoxication.
abstract: Organisms have sophisticated subcellular compartments containing enzymes that function in tandem. These confined compartments ensure effective chemical transformation and transport of molecules, and the elimination of toxic metabolic wastes. Creating functional enzyme complexes that are confined in a similar way remains challenging. Here we show that two or more enzymes with complementary functions can be assembled and encapsulated within a thin polymer shell to form enzyme nanocomplexes. These nanocomplexes exhibit improved catalytic efficiency and enhanced stability when compared with free enzymes. Furthermore, the co-localized enzymes display complementary functions, whereby toxic intermediates generated by one enzyme can be promptly eliminated by another enzyme. We show that nanocomplexes containing alcohol oxidase and catalase could reduce blood alcohol levels in intoxicated mice, offering an alternative antidote and prophylactic for alcohol intoxication.
authors: Key Laboratory of Functional Polymer Materials, Ministry of Education, and Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
authors: Yang
authors: Y
authors: Liu
authors: Juanjuan
authors: J
authors: Du
authors: Ming
authors: M
authors: Yan
authors: Mo Yin
authors: MY
authors: Lau
authors: Jay
authors: J
authors: Hu
authors: Hui
authors: H
authors: Han
authors: Otto O
authors: OO
authors: Yang
authors: Sheng
authors: S
authors: Liang
authors: Wei
authors: W
authors: Wei
authors: Hui
authors: H
authors: Wang
authors: Jianmin
authors: J
authors: Li
authors: Xinyuan
authors: X
authors: Zhu
authors: Linqi
authors: L
authors: Shi
authors: Wei
authors: W
authors: Chen
authors: Cheng
authors: C
authors: Ji
authors: Yunfeng
authors: Y
authors: Lu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry; D000435:Alcoholic Intoxication / Q000188:drug therapy* / Q000473:pathology; D000438:Alcohols / Q000008:administration & dosage / Q000097:blood; D000818:Animals; D000931:Antidotes / Q000008:administration & dosage*; D032701:Biomimetics; D002374:Catalase / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry*; D051379:Mice
chemical_list: D000438:Alcohols; D000931:Antidotes; D046911:Macromolecular Substances; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D002374:Catalase