title: Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum: a modification of the virus neutralization test.
abstract: To eliminate cytotoxic effects of colostrum on cells, a modified virus neutralization test (VNT) for the detection of Bovine viral diarrhea virus-specific neutralizing antibodies in colostrum was developed. The new test was compared to the World Organization for Animal Health-recommended VNT and the results evaluated. The agreement of the new test compared to the standard VNT was determined to be 98%, whereas sensitivity and specificity of the modified VNT compared to the standard VNT were 100%. Bovine viral diarrhea virus-specific antibodies were detected in 42 sera samples and 38 colostrum samples. The antibody titers in serum and colostrum showed a high correlation (n = 56, r = 0.9719, P < 0.001). The modified virus neutralization technique described herein succeeds in eliminating cytotoxic effects and can be readily applied for the detection of specific antibodies against other infectious agents in colostrum.
authors: Croatian Veterinary Institute, Zagreb, Croatia. bedekovic@veinst.hr
authors: Tomislav
authors: T
authors: Bedekovic
authors: Zeljko
authors: Z
authors: Mihaljevic
authors: Andreja
authors: A
authors: Jungic
authors: Nina
authors: N
authors: Lemo
authors: Ivana
authors: I
authors: Lojkic
authors: Zeljko
authors: Z
authors: Cvetnic
authors: Zeljko
authors: Z
authors: Cac
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D001908:Diarrhea Viruses, Bovine Viral / Q000737:chemistry*; D009500:Neutralization Tests / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral