title: Loss of the major Type I arginine methyltransferase PRMT1 causes substrate scavenging by other PRMTs.
abstract: Arginine methylation is a common posttranslational modification that is found on both histone and non-histone proteins. Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation. Here, we show that with the loss of PRMT1 activity, there are major increases in global MMA and SDMA levels, as detected by type-specific antibodies. Amino acid analysis confirms that MMA and SDMA levels accumulate when ADMA levels are reduced. These findings reveal the dynamic interplay between different arginine methylation types in the cells, and that the pre-existence of the dominant ADMA mark can block the occurrence of SDMA and MMA marks on the same substrate. This study provides clear evidence of competition for different arginine methylation types on the same substrates.
authors: Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX 78957, USA.
authors: Surbhi
authors: S
authors: Dhar
authors: Vidyasiri
authors: V
authors: Vemulapalli
authors: Alexander N
authors: AN
authors: Patananan
authors: Grace L
authors: GL
authors: Huang
authors: Alessandra
authors: A
authors: Di Lorenzo
authors: Stephane
authors: S
authors: Richard
authors: Michael J
authors: MJ
authors: Comb
authors: Ailan
authors: A
authors: Guo
authors: Steven G
authors: SG
authors: Clarke
authors: Mark T
authors: MT
authors: Bedford
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D055785:Gene Knockdown Techniques; D006801:Humans; D008745:Methylation; D011484:Protein-Arginine N-Methyltransferases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D012097:Repressor Proteins; C018524:N,N-dimethylarginine; D001120:Arginine; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases