title: Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase.
abstract: The Tat machinery translocates fully-folded and oligomeric substrates. The passage of large, bulky cargos across an ion-tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre-SufI as a base. Four (of 17) cargos transported with significant (>20% of wild-type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability.
authors: Department of Molecular and Cellular Medicine, College of Medicine, The Texas A&M Health Science Center, 1114 TAMU, College Station, TX 77843, USA.
authors: Neal
authors: N
authors: Whitaker
authors: Umesh
authors: U
authors: Bageshwar
authors: Siegfried M
authors: SM
authors: Musser
mesh_terms: D001692:Biological Transport; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D003545:Cysteine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008970:Molecular Weight; D009154:Mutation; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D011498:Protein Precursors; C473632:SufI protein, E coli; C499697:TatA protein, E coli; C473116:TatB protein, E coli; C461379:TatC protein, E coli; D049452:Green Fluorescent Proteins; D003545:Cysteine