title: Polymers for the cell-specific immobilisation of megakaryocytic cell lines.
abstract: Primary human megakaryocytes, the precursor cells of platelets, are difficult to collect and cultivate. Polymers that enrich these cells without affecting their regulation or function are of interest for basic research as well as for cord blood transplantation purposes since co-transplantation of enriched megakaryocyte concentrates increase the success of stem cell therapy. Herein, polymer microarrays were used for the discovery of substrates for MEG-01 cells, with five polymers identified which selectively bound cells of the megakaryocytic lineage. Flow cytometry and miRNA profiling revealed that immobilisation had only a minor effect on the cellular maturation status, making the identified substrates potential candidates for concentrating megakaryocytes from patients prior to transplantation.
authors: School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh EH93JJ, UK.
authors: Anne
authors: A
authors: Hansen
authors: Samuel
authors: S
authors: Corless
authors: Alexander
authors: A
authors: Cleland
authors: Juraj
authors: J
authors: Petrik
authors: Nick
authors: N
authors: Gilbert
authors: Mark
authors: M
authors: Bradley
mesh_terms: D000179:Acrylates / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D018914:Cells, Immobilized / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D013058:Mass Spectrometry; D008533:Megakaryocytes / Q000378:metabolism*; D046228:Microarray Analysis; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011140:Polyurethanes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000179:Acrylates; D011140:Polyurethanes