title: Expression of Phosphoinositide-specific phospholipase C enzymes in human osteosarcoma cell lines.
abstract: The definition of the number and nature of signal transduction pathways networking in the pathogenesis of osteosarcoma raised great interest. Intracellular calcium ions are important second messengers implicated in the control of cell death. The calcium concentration is regulated by signal transduction pathways, including the Phosphoinositides (PI) signaling. Phosphatydil inositol (4,5) bisphosphate (PIP2) is critical for many cellular activities. The levels of PIP2 are regulated by means of Phosphoinositide-specific Phospholipase C (PI-PLC) family of enzymes. We delineated the panel of expression of PI-PLC enzymes in four human osteosarcoma cell lines. In MG-63 cell line, PI-PLC β1, β2, β3, β4, γ1, γ2, δ1, δ3 and ε resulted expressed. In 143B cell line, PI-PLC β1, β2, β3, β4, γ1, γ2, δ1, δ3 and ε were expressed. In SaOS-2 cell line, PI-PLC β1, β3, β4, γ1, γ2, δ1, δ3, ε and η1. In Hs888 cell line, PI-PLC β1, β3, β4, γ1, δ1, δ3, δ4, ε and η1 the administration of U-73122 to cultures briefly modifies the levels of PI-PLC transcripts. The obtained complete expression panel of PI-PLC isoforms will be a useful tool for further functional studies about the role of the PI signal transduction pathway in osteosarcoma.
authors: Department of Sensitive Organs, Policlinic Umberto I, Faculty of Medicine and Odontoiatry, Sapienza University of Rome, viale del Policlinico 155, Rome, 00185, Italy, ritalovasco@hotmail.it.
authors: Vincenza Rita
authors: VR
authors: Lo Vasco
authors: Martina
authors: M
authors: Leopizzi
authors: Caterina
authors: C
authors: Chiappetta
authors: Chiara
authors: C
authors: Puggioni
authors: Claudio
authors: C
authors: Di Cristofano
authors: Carlo
authors: C
authors: Della Rocca