title: Kinetics of ozone inactivation of infectious prion protein.
abstract: The kinetics of ozone inactivation of infectious prion protein (PrP(Sc), scrapie 263K) was investigated in ozone-demand-free phosphate-buffered saline (PBS). Diluted infectious brain homogenates (IBH) (0.01%) were exposed to a predetermined ozone dose (10.8 ± 2.0 mg/liter) at three pHs (pH 4.4, 6.0, and 8.0) and two temperatures (4°C and 20°C). The inactivation of PrP(Sc) was quantified by determining the in vitro destruction of PrP(Sc) templating properties using the protein misfolding cyclic amplification (PMCA) assay and bioassay, which were shown to correlate well. The inactivation kinetics were characterized by both Chick-Watson (CW) and efficiency factor Hom (EFH) models. It was found that the EFH model fit the experimental data more appropriately. The efficacy of ozone inactivation of PrP(Sc) was both pH and temperature dependent. Based on the EFH model, CT (disinfectant concentration multiplied by contact time) values were determined for 2-log10, 3-log10, and 4-log10 inactivation at the conditions under which they were achieved. Our results indicated that ozone is effective for prion inactivation in ozone-demand-free water and may be applied for the inactivation of infectious prion in prion-contaminated water and wastewater.
authors: Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada.
authors: Ning
authors: N
authors: Ding
authors: Norman F
authors: NF
authors: Neumann
authors: Luke M
authors: LM
authors: Price
authors: Shannon L
authors: SL
authors: Braithwaite
authors: Aru
authors: A
authors: Balachandran
authors: Gordon
authors: G
authors: Mitchell
authors: Miodrag
authors: M
authors: Belosevic
authors: Mohamed
authors: M
authors: Gamal El-Din
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D006224:Cricetinae; D004202:Disinfectants; D006863:Hydrogen-Ion Concentration; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D018620:PrPSc Proteins / Q000737:chemistry / Q000187:drug effects*; D017510:Protein Folding; D012608:Scrapie / Q000378:metabolism
chemical_list: D004202:Disinfectants; D010083:Oxidants, Photochemical; D018620:PrPSc Proteins; D010126:Ozone