title: Acyl spermidines in inflorescence extracts of elder (Sambucus nigra L., Adoxaceae) and elderflower drinks.
abstract: LC-UV-MS analyses of inflorescence extracts of Sambucus nigra L. (elder, Adoxaceae) revealed the presence of numerous acyl spermidines, with isomers of N,N-diferuloylspermidine and N-acetyl-N,N-diferuloylspermidine being most abundant. Pollen was the main source of the acyl spermidines in the inflorescence. Three of the major acyl spermidines were isolated and their structures determined by NMR spectroscopy as N⁵,N¹⁰-di-(E,E)-feruloylspermidine and the new compounds N¹-acetyl-N⁵,N¹⁰-di-(Z,E)-feruloylspermidine and N¹-acetyl-N⁵,N¹⁰-di-(E,E)-feruloylspermidine. An isomer of N,N,N-triferuloylspermidine was also obtained and identified as N¹,N⁵,N¹⁰-tri-(E,E,E)-feruloylspermidine. In addition to stereoisomers of the isolated acyl spermidines, other acyl spermidines detected by the positive ion LC-UV-MS were isomers of N-caffeoyl-N,N-diferuloylspermidine, N-coumaroyl-N,N-diferuloylspermidine, N-caffeoyl-N-feruloylspermidine, N-coumaroyl-N-feruloylspermidine, N-acetyl-N-caffeoyl-N-feruloylspermidine, and N-acetyl-N-coumaroyl-N-feruloylspermidine. Analysis of commercial elderflower drinks showed that acyl spermidines were persistent in these processed elderflower products. Examination of inflorescence extracts from Sambucus canadensis L. (American elder) revealed the presence of acyl spermidines that were different from those of S. nigra.
authors: Royal Botanic Gardens, Kew, Richmond, Surrey, United Kingdom.
authors: Geoffrey C
authors: GC
authors: Kite
authors: Sonny
authors: S
authors: Larsson
authors: Nigel C
authors: NC
authors: Veitch
authors: Elaine A
authors: EA
authors: Porter
authors: Ning
authors: N
authors: Ding
authors: Monique S J
authors: MS
authors: Simmonds
mesh_terms: D000107:Acetylation; D000215:Acylation; D001628:Beverages / Q000032:analysis*; D056626:Inflorescence / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry; D027924:Sambucus nigra / Q000737:chemistry*; D013095:Spermidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D006113:United Kingdom
chemical_list: D010936:Plant Extracts; D013095:Spermidine