title: Genetic variants in HLA-DP/DQ contribute to risk of cervical cancer: a two-stage study in Chinese women.
abstract: OBJECTIVE
  Human leukocyte antigens (HLA) play an important role in presenting virus antigens to immune cells that are responsible for the clearance of virus-infected cells and tumor cells. Herein, we evaluated whether genetic variants of HLA-DP and HLA-DQ are associated with cervical cancer risk.
  
  
  METHODS
  We genotyped four single nucleotide polymorphisms (SNPs) in HLA-DP (rs3077 and rs9277535) and HLA-DQ (rs2856718 and rs7453920) in a two-stage case-control study with a total of 2317 cervical cancer cases and 2109 cancer-free controls using TaqMan allelic discrimination assay.
  
  
  RESULTS
  We found consistently significant associations of HLA-DP rs3077 and rs9277535 with increased risks of cervical cancer (dominant genetic model: adjusted OR=1.51, 95% CI=1.32-1.71 for rs3077; adjusted OR=1.29, 95% CI=1.12-1.49 for rs9277535). When combining the effects of HLA-DP rs3077 and rs9277535, subjects carrying "≥1" variant alleles had a 1.55-fold increased risk of cervical cancer (95% CI=1.32-1.81), compared with those carrying "0" variant allele. And cervical cancer risk significantly increased with the increasing number of variant alleles of the two SNPs in a dose-dependent manner (P for trend=4.33×10(-10)). However, there were no significant associations for HLA-DQ rs2856718 and rs7453920 in our population.
  
  
  CONCLUSIONS
  These findings indicate that HLA-DP rs3077 and rs9277535 were candidate susceptibility markers for cervical cancer in Chinese females. Further validation studies with different ethnic background, biological function analyses and especially HPV typing together were needed.
authors: MOE Key Laboratory of Modern Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.
authors: Jie
authors: J
authors: Jiang
authors: Ni
authors: N
authors: Li
authors: Yan
authors: Y
authors: Shen
authors: Jibin
authors: J
authors: Liu
authors: Li
authors: L
authors: Liu
authors: Jiangbo
authors: J
authors: Du
authors: Yu
authors: Y
authors: Lei
authors: Yang
authors: Y
authors: Wen
authors: Lin
authors: L
authors: Yang
authors: Lanwei
authors: L
authors: Guo
authors: Kai
authors: K
authors: Zhang
authors: Fulin
authors: F
authors: Qiang
authors: Sumin
authors: S
authors: Wang
authors: Zhibin
authors: Z
authors: Hu
authors: Min
authors: M
authors: Dai
authors: Hongbing
authors: H
authors: Shen
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D002277:Carcinoma / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006682:HLA-DP Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008875:Middle Aged; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D002583:Uterine Cervical Neoplasms / Q000208:ethnology / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens