title: A new haplotype variability in complement C6 is marginally associated with resistance to Aeromonas hydrophila in grass carp.
abstract: Aeromonas hydrophila, a widespread bacterium in the aquatic environment, causes haemorrhagic septicemia in fish. In the last decade, the disease has caused mass mortality and tremendous economic loss in cultured grass carp in the mainland China. The complement component C6 is a constituent of a biochemical cascade that serves as a major effector of the human innate and adaptor immunity, and eliminates infected cells. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the C6 gene and to assess their association with A. hydrophila resistance in grass carp. A resource population consisting of 186 susceptible and 191 resistant grass carp was constructed. The gcC6 genomic sequence is composed of 9292 bp, containing 18 exons and 17 introns. The promoter sequence of gcC6 gene contained several consensus sequences for hepatic-specific transcription factors. We sequenced a total of 9744 bp of the C6 gene from a diverse population of grass carp and identified 8 SNPs that were genotyped in the resource population. Statistical analysis revealed a lack of association between any individual SNPs and resistance to A. hydrophila in grass carp. The SNPs 1214G>A, 1380G>C, 2095A>C and 2167T>C were linked together (r(2) > 0.8). The haplotype GCCC generated with these four SNPs was associated marginally with resistance to A. hydrophila in grass carp. These findings suggest a lack of strong association of the C6 polymorphisms with the A. hydrophila resistance in grass carp.
authors: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.
authors: Yubang
authors: Y
authors: Shen
authors: Junbin
authors: J
authors: Zhang
authors: Xiaoyan
authors: X
authors: Xu
authors: Jianjun
authors: J
authors: Fu
authors: Jiale
authors: J
authors: Li
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002347:Carps; D002681:China; D003001:Cloning, Molecular; D003183:Complement C6 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis; D060467:Disease Resistance; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D006239:Haplotypes; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000662:veterinary
chemical_list: D003183:Complement C6; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger