title: Effects of low doses of bisphenol A on the metabolome of perinatally exposed CD-1 mice.
abstract: BACKGROUND
  Bisphenol A (BPA) is a well-known endocrine disruptor used to manufacture polycarbonate plastics and epoxy resins. Exposure of pregnant rodents to low doses of BPA results in pleiotropic effects in their offspring.
  
  
  OBJECTIVE
  We used metabolomics--a method for determining metabolic changes in response to nutritional, pharmacological, or toxic stimuli--to examine metabolic shifts induced in vivo by perinatal exposure to low doses of BPA in CD-1 mice.
  
  
  METHODS
  Male offspring born to pregnant CD-1 mice that were exposed to vehicle or to 0.025, 0.25, or 25 µg BPA/kg body weight/day, from gestation day 8 through day 16 of lactation, were examined on postnatal day (PND) 2 or PND21. Aqueous extracts of newborns (PND2, whole animal) and of livers, brains, and serum samples from PND21 pups were submitted to (1)H nuclear magnetic resonance spectroscopy. Data were analyzed using partial least squares discriminant analysis.
  
  
  RESULTS
  Examination of endogenous metabolic fingerprints revealed remarkable discrimination in whole extracts of the four PND2 newborn treatment groups, strongly suggesting changes in the global metabolism. Furthermore, statistical analyses of liver, serum, and brain samples collected on PND21 successfully discriminated among treatment groups. Variations in glucose, pyruvate, some amino acids, and neurotransmitters (γ-aminobutyric acid and glutamate) were identified.
  
  
  CONCLUSIONS
  Low doses of BPA disrupt global metabolism, including energy metabolism and brain function, in perinatally exposed CD-1 mouse pups. Metabolomics can be used to highlight the effects of low doses of endocrine disruptors by linking perinatal exposure to changes in global metabolism.
authors: Institut National de la Recherche Agronomique, UMR1331, TOXALIM (Research Centre in Food Toxicology), Toulouse, France.
authors: Nicolas J
authors: NJ
authors: Cabaton
authors: Cécile
authors: C
authors: Canlet
authors: Perinaaz R
authors: PR
authors: Wadia
authors: Marie
authors: M
authors: Tremblay-Franco
authors: Roselyne
authors: R
authors: Gautier
authors: Jérôme
authors: J
authors: Molina
authors: Carlos
authors: C
authors: Sonnenschein
authors: Jean-Pierre
authors: JP
authors: Cravedi
authors: Beverly S
authors: BS
authors: Rubin
authors: Ana M
authors: AM
authors: Soto
authors: Daniel
authors: D
authors: Zalko
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000187:drug effects*; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D051379:Mice; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D019344:Lactic Acid; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds