title: Ethanol production from D-lactic acid by lactic acid-assimilating Saccharomyces cerevisiae NAM34-4C.
abstract: The lactic acid-assimilating yeast Saccharomyces cerevisiae NAM34-4C grew rapidly in minimal D-lactate medium (pH 3.5) at 35°C, compared with minimal L-lactate medium. A laboratory strain, S. cerevisiae S288C, did not grow in either medium at pH 3.5. Strain NAM34-4C produced remarkably high levels of ethanol in YPDL medium at pH 3.5, but not at pH 5.5, when D-lactate was provided as the carbon source. Optimal cultivation conditions for ethanol production from D-lactate by strain NAM34-4C were as follows: shaking speed, 60 rpm; initial pH, 3.0; cultivation temperature, 35°C; yeast extract, 5 g/L; peptone, 10 g/L; and D-lactate, 30 g/L. Under these conditions, strain NAM34-4C produced 2.7 g/L ethanol, which is 18% of the theoretical maximal yield (0.51 3 initial D-lactate concentration).
authors: Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors: Makoto
authors: M
authors: Wakamatsu
authors: Tatsunori
authors: T
authors: Tani
authors: Hisataka
authors: H
authors: Taguchi
authors: Masayoshi
authors: M
authors: Matsuoka
authors: Kenji
authors: K
authors: Kida
authors: Takashi
authors: T
authors: Akamatsu
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D000431:Ethanol