title: Effects of systemically transplanted allogeneic bone marrow multipotent mesenchymal stromal cells on rats' recovery after experimental polytrauma.
abstract: BACKGROUND
  The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells (BM MMSCs) on recovery after polytrauma and bone fracture repair.
  
  
  METHODS
  A total 27 Wistar-Kyoto rats were divided into three groups (n = 9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSC transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours 9 days after surgery, nine rats received allogeneic BM MMSCs (1 × 10(6) cells per kilogram) intravenously. The day before operation and at Days 3 and 10 after surgery as well as at the end of the experiment, blood analysis was carried out. At 10, 20, and 30 days after surgery the rats' locomotor activity was assessed in an open-field test. At Day 30, rats were euthanized, and macroscopic and histologic observations of rats' lower extremities was performed.
  
  
  RESULTS
  The treated animals gained weight faster regained their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios, as well as less marked muscle atrophy.Rat treatment with BM MMSC transplantation stimulated bone fracture healing-bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae.Red blood cell analysis results showed delayed recovery after hemorrhage in the rats receiving allogeneic BM MMSCs: restoration of red blood cell counts, hematocrit level, and hemoglobin level was slower in the untreated animals.
  
  
  CONCLUSIONS
  Allogeneic BM MMSC transplantation improved rats rehabilitation scores after experimental polytrauma.
authors: Institute of Innovative Biomedical Technology, University of Latvia, Riga, Latvia. guntra.krumina@gmail.com
authors: Guntra
authors: G
authors: Krumina
authors: Dmitry
authors: D
authors: Babarykin
authors: Zaiga
authors: Z
authors: Krumina
authors: Irina
authors: I
authors: Paegle
authors: Oleg
authors: O
authors: Suhorukov
authors: Dmitry
authors: D
authors: Vanags
authors: Galina
authors: G
authors: Makarenkova
authors: Sergey
authors: S
authors: Nikulshin
authors: Inese
authors: I
authors: Folkmane
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002146:Bony Callus / Q000637:transplantation; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009104:Multiple Trauma / Q000473:pathology / Q000601:surgery*; D051381:Rats; D011921:Rats, Inbred WKY; D020127:Recovery of Function / Q000502:physiology*; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology*