title: Redox-proteomic analysis of doxorubicin resistance-induced altered thiol activity in uterine carcinoma.
abstract: Doxorubicin is an anticancer drug used in a wide range of cancer therapies; however, doxorubicin-induced drug resistance is one of the most serious obstacles of cancer chemotherapy. Recent studies have indicated that reduced oxidative stress levels in cancer cells induce drug resistance. However, the redox-modifications of resistance - associated cellular targets are largely unknown. Thus, the current study employed cysteine-labeling based two-dimensional differential gel electrophoresis (2D-DIGE) combined with MALDI-TOF mass spectrometry (MALDI-TOF MS) to analyze the effect of doxorubicin resistance on redox regulation in uterine cancer and showed 33 spots that were significantly changed in thiol reactivity. These proteins involve cytoskeleton regulation, signal transduction, redox-regulation, glycolysis, and cell-cycle regulation. The current work shows that the redox 2D-DIGE-based proteomic strategy provides a rapid method to study the molecular mechanisms of doxorubicin-induced drug resistance in uterine cancer. The identified targets may be used to further evaluate their roles in drug-resistance formation and for possible diagnostic or therapeutic applications.
authors: Department of Medical Science and Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan.
authors: Szu-Ting
authors: ST
authors: Lin
authors: Yi-Wen
authors: YW
authors: Lo
authors: Shing-Jyh
authors: SJ
authors: Chang
authors: Wen-Ching
authors: WC
authors: Wang
authors: Margaret Dah-Tsyr
authors: MD
authors: Chang
authors: Ping-Chiang
authors: PC
authors: Lyu
authors: Yi-Wen
authors: YW
authors: Chen
authors: Hsiu-Chuan
authors: HC
authors: Chou
authors: Hong-Lin
authors: HL
authors: Chan
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000627:therapeutic use*; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D010084:Oxidation-Reduction; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D004317:Doxorubicin