title: Analyzing arthropods for the presence of bacteria.
abstract: Bacteria within arthropods can be identified using culture-independent methods. This unit describes protocols for surface sterilization of arthropods, DNA extraction of whole bodies and tissues, touchdown PCR amplification using 16S rDNA general bacteria primers, and profiling the bacterial community using denaturing gradient gel electrophoresis.
authors: Department of Entomology, University of Kentucky, Lexington, Kentucky, USA.
authors: Elizabeth S
authors: ES
authors: Andrews
mesh_terms: D000818:Animals; D001181:Arthropods / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D056186:Metagenomics / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S