title: Comparative expression profile of orphan receptor tyrosine kinase ROR1 in Iranian patients with lymphoid and myeloid leukemias.
abstract: It has recently been shown that ROR1, a member of the receptor tyrosine kinase family, is overexpressed in leukemic B cells of Chronic Lymphocytic Leukemia (CLL) and a subset of Acute Lymphoblastic Leukemia (ALL). In this comparative study the expression profile of ROR1 mRNA was investigated in Iranian patients with CLL and Acute Myelogenous Leukemia (AML) and the results were compared with those previously reported in our Iranian ALL patients. RT-PCR was performed on bone marrow and/or peripheral blood samples of 84 CLL and 12 AML patients. CLL samples were classified into immunoglobulin heavy chain variable region (IGHV) gene mutated (n = 55) and unmutated (n = 29) and also indolent (n = 42) and progressive (n = 39) subtypes. ROR1 expression was identified in 94% of our CLL patients, but none of the AML patients expressed ROR1. No significant differences were observed between different CLL subtypes for ROR1 expression. Taken together the present data and our previous results on ROR1 expression in ALL, our findings propose ROR1 as a tumor-associated antigen overexpressed in a large proportion of lymphoid (CLL and ALL), but not myeloid (AML) leukemias. Expression of ROR1 seems to be associated to lineage and differentiation stages of leukemic cells with a potential implication for immunotherapy.
authors: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mahdi
authors: M
authors: Shabani
authors: Hossein
authors: H
authors: Asgarian Omran
authors: Mohammad Hojjat
authors: MH
authors: Farsangi
authors: Parvaneh
authors: P
authors: Vossough
authors: Ramazan A
authors: RA
authors: Sharifian
authors: Gholam R
authors: GR
authors: Toughe
authors: Seyed Mohsen
authors: SM
authors: Razavi
authors: Jalal
authors: J
authors: Khoshnoodi
authors: Mahmood
authors: M
authors: Jeddi-Tehrani
authors: Hodjatallah
authors: H
authors: Rabbani
authors: Fazel
authors: F
authors: Shokri
keywords: Acute myelogenous leukemia; Chronic lymphocytic leukemia; ROR1; RT-PCR