title: Cytoprotective effect of epigallocatechin-3-gallate against deoxynivalenol-induced toxicity through anti-oxidative and anti-inflammatory mechanisms in HT-29 cells.
abstract: Deoxynivalenol (DON) is a mycotoxin produced by Fusarium sp., and is known to elicit pro-inflammatory responses in the cell. The cells exposed to DON undergo apoptosis as a mechanism to shut down the inflammation. In this study, we tested the cytoprotective effect of the green tea polyphenol epigallocatechin 3-gallate (EGCG) on DON-induced toxicity in HT-29 cells. EGCG prevented DON-induced cytotoxicity to HT-29 cells in a dose-response manner. Even the lowest concentration (5 μM) of EGCG showed protection against the highest concentration of DON tried (3.38 μM=1000 ng/ml). Our study also demonstrates that IC20 value of DON in HT 29 cells were 250 ng/ml and pre-treatment with 20 μM EGCG yielded 99% cell viability. EGCG also protected against oxidative stress, up regulation of nuclear factor-kB (NF-κB), cyclooxygenase-2 (COX-2) and caspase-3 activated apoptosis. These results suggest that EGCG acts as cytoprotective agent against DON-induced toxicity.
authors: Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
authors: Palaniswamy
authors: P
authors: Kalaiselvi
authors: Krishnaswamy
authors: K
authors: Rajashree
authors: Lohanathan
authors: L
authors: Bharathi Priya
authors: Viswanadha Vijaya
authors: VV
authors: Padma
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D019610:Cytoprotection; D005670:Fusarium / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000494:pharmacology; D013662:Tea / Q000737:chemistry; D014255:Trichothecenes / Q000633:toxicity*; D015854:Up-Regulation
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016328:NF-kappa B; D059808:Polyphenols; D013662:Tea; D014255:Trichothecenes; D002392:Catechin; C045651:epigallocatechin gallate; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C505407:CASP3 protein, human; D053148:Caspase 3; C007262:deoxynivalenol