title: miR-154 inhibits prostate cancer cell proliferation by targeting CCND2.
abstract: BACKGROUND
  Research has shown reduced expression levels of miR-154 in prostate cancer (CaP). However, the function and molecular mechanisms of miR-154 in this cancer type remains unknown.
  
  
  OBJECTIVE
  The aims of this study were to examine the functional significance of miR-154 in CaP cells and to identify the novel molecular targets regulated by miR-154.
  
  
  METHODS
  miR-154 expression significantly decreased in primary CaP samples compared with nonmalignant samples measured by quantitative reverse transcription polymerase chain reaction. Restoration of miR-154 lowered the potential of CaP cell lines to grow and proliferate in vitro evaluated by CCK-8 assay, colony formation assay, and flow cytometry. miR-154 down-regulated the expression of CCND2 by binding to its 3'-untranslated region by luciferase reporter assay.
  
  
  CONCLUSIONS
  miR-154 plays a prominent role in CaP proliferation by suppressing CCND2, and it may provide a new approach to the treatment of CaP.
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Chen
authors: C
authors: Zhu
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Pengfei
authors: P
authors: Shao
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Meiling
authors: M
authors: Bao
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Pu
authors: P
authors: Li
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Hai
authors: H
authors: Zhou
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Hongzhou
authors: H
authors: Cai
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Qiang
authors: Q
authors: Cao
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Liangjun
authors: L
authors: Tao
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Xiaoxin
authors: X
authors: Meng
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Xiaobing
authors: X
authors: Ju
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Chao
authors: C
authors: Qin
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address: lijie203076@yahoo.com.
authors: Jie
authors: J
authors: Li
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address: drcjyin@gmail.com.
authors: Changjun
authors: C
authors: Yin
mesh_terms: D000368:Aged; D002453:Cell Cycle; D049108:Cell Enlargement; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056742:Cyclin D2 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: C080336:CCND2 protein, human; D056742:Cyclin D2; C561122:MIRN154 microRNA, human; D035683:MicroRNAs
keywords: CCND2; Prostate cancer; miR-154