title: The history and advances of reversible terminators used in new generations of sequencing technology.
abstract: DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the "REAP" method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application.
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. chenfei@big.ac.cn
authors: Fei
authors: F
authors: Chen
authors: Mengxing
authors: M
authors: Dong
authors: Meng
authors: M
authors: Ge
authors: Lingxiang
authors: L
authors: Zhu
authors: Lufeng
authors: L
authors: Ren
authors: Guocheng
authors: G
authors: Liu
authors: Rong
authors: R
authors: Mu
mesh_terms: D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics / Q000379:methods; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D017422:Sequence Analysis, DNA / Q000191:economics / Q000266:history / Q000379:methods*
chemical_list: D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase