title: Transcriptional activity of an  ovarian-specific promoter from rat in dairy goat granulosa cells.
abstract: Ovarian-specific  promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary  DNA library of rat that has been thought to be specifically expressed in ovary.  To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from  rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding  for enhanced green fluorescent protein (EGFP) was used as a reporter to examine  the activity of OSP-1 in GCs. EGFP was successfully expressed in dairy goat GCs  transfected with pOSP-1-EGFP. Reverse transcriptase-polymerase chain reaction  analysis confirmed the tissue-specific transcription of EGFP messenger RNA in  dairy goat GCs transfected with pOSP-1-EGFP. We concluded that OSP-1 promoter  from rat can specifically drive foreign gene expression in dairy goat GCs.  Thus, we obtained a tissue-specific regulation element and provided a potential  tool for the research of regulation and development of the ovary in dairy  goats.
authors: Department of Animal Science and Technology, Northwest A&F University, Yangling, China.
authors: H-Y
authors: HY
authors: Liu
authors: M-M
authors: MM
authors: Yang
authors: Y-H
authors: YH
authors: Cui
authors: T
authors: T
authors: Ma
authors: X-Q
authors: XQ
authors: Liu
authors: L
authors: L
authors: Bai
authors: W-P
authors: WP
authors: Xiao
authors: H-B
authors: HB
authors: Zhao
authors: J-Y
authors: JY
authors: Peng
authors: X-B
authors: XB
authors: Hu
authors: B-Y
authors: BY
authors: Cao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D057167:Claudins / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D006107:Granulosa Cells / Q000378:metabolism / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D010053:Ovary / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D018626:Retroelements; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods
chemical_list: D057167:Claudins; C492353:Cldn11 protein, rat; D018076:DNA, Complementary; D012333:RNA, Messenger; D018626:Retroelements; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins