title: [Oral fluid and urine as alternative samples in confirmatory testing of HIV-1 infection].
abstract: The objective of this report was to evaluate oral fluid and urine as optional samples for HIV-1 confirmatory test using DAVIH-BLOT system (Laboratorios DAVIH, La Habana, Cuba), for which they were compared with their corresponding serum samples in a group of 125 individuals. In band pattern analysis, predominant antibodies in positives oral fluid and urine samples against p34, p68, gp41, gp120, were no different from their corresponding sera according to the proportion comparison test (p < 0,001). Relative sensitivity and specificity of this system were 100% in oral fluid and 98, 75% and 100% in urine samples respectively. These results support optional use of oral fluid and urine that, with slight modifications in the diagnostic system, can be applied for HIV-1 antibody confirmation testing.
authors: Laboratorio de Investigaciones Del Sida (LISIDA). cicdc@infomed.sld.cu
authors: Otto
authors: O
authors: Cruz Sui
authors: María Teresa
authors: MT
authors: Pérez Guevara
authors: Ana Luisa
authors: AL
authors: Lubián Caballero
authors: Lucy
authors: L
authors: Montano Tamayo
authors: Eladio
authors: E
authors: Silva Cabrera
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods*; D000918:Antibody Specificity; D015153:Blotting, Western / Q000379:methods*; D015483:HIV Antibodies / Q000032:analysis* / Q000652:urine; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis* / Q000378:metabolism / Q000652:urine; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D012463:Saliva / Q000276:immunology*; D012680:Sensitivity and Specificity; D016482:Urinalysis
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D007074:Immunoglobulin G