title: Analysis of p300 occupancy at the early stage of stem cell differentiation by chromatin immunoprecipitation.
abstract: Chromatin immunoprecipitation (ChIP) is an invaluable method to study the specific interaction of regulatory proteins with genomic DNA. Since its first development, it has been modified extensively to make it applicable to many different cell types and experimental systems. The cross-linking of regulatory proteins to genomic DNA requires monolayer cells or single cell suspensions. Here, we describe a ChIP protocol using embryoid bodies formed at the early stage differentiation of pluripotent stem cells, which we have used to determine long-range p300-dependent regulatory elements of myogenic-specific genes.
authors: Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, ON, Canada.
authors: Melanie
authors: M
authors: Le May
authors: Qiao
authors: Q
authors: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D058732:Embryoid Bodies / Q000502:physiology; D005557:Formaldehyde / Q000737:chemistry; D006801:Humans; D011485:Protein Binding; D013010:Sonication; D013234:Stem Cells / Q000502:physiology*; D050880:p300-CBP Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D005557:Formaldehyde; D004247:DNA; D050880:p300-CBP Transcription Factors