title: Hydrophobically modified quaternized celluloses as new dynamic coatings in CE for basic protein separation.
abstract: Hydrophobically modified quaternized celluloses (HMQCs), containing the quaternary ammonium groups and hexadecyl groups, were homogeneously synthesized as novel dynamic coatings for CE. Compared with quaternized cellulose coating, HMQC coating is able to generate stronger reversed EOF (ca. 10% increase) and has better efficiency in suppressing protein adsorption. The effects of the polymer concentration, the degree of hydrophobic substitution, and the buffer pH on the EOF mobility as well as on the separation of basic proteins were investigated in detail. It was shown that the use of HMQC coating could obviously improve the separation performance of basic proteins within a broad pH range. Five basic proteins (lysozyme, ribonuclease A, cytochrome c, bovine pancreatic trypsin inhibitor, and chymotrypsinogen) could be completely separated at pH 8.0. The successful performance of HMQC was further demonstrated by the analyses of lysozyme in tear and urine samples.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, P. R. China.
authors: Lingguo
authors: L
authors: Zhao
authors: Jinping
authors: J
authors: Zhou
authors: Haitao
authors: H
authors: Zhou
authors: Qin
authors: Q
authors: Yang
authors: Ping
authors: P
authors: Zhou
mesh_terms: D000328:Adult; D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D009113:Muramidase / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013499:Surface Properties; D013666:Tears / Q000737:chemistry
chemical_list: D011506:Proteins; D002482:Cellulose; D009113:Muramidase