title: Deorphanization and target validation of cross-tick species conserved novel Amblyomma americanum tick saliva protein.
abstract: We previously identified a cross-tick species conserved tick feeding stimuli responsive Amblyomma americanum (Aam) AV422 gene. This study demonstrates that AamAV422 belongs to a novel group of arthropod proteins that is characterized by 14 cysteine amino acid residues: C(23)-X7/9-C(33)-X23/24-C(58)-X8-C(67)-X7-C(75)-X23-C(99)-X15-C(115)-X10-C(126)-X24/25/33-C(150)C(151)-X7-C(159)-X8-C(168)-X23/24-C(192)-X9/10-C(202) predicted to form seven disulfide bonds. We show that AamAV422 protein is a ubiquitously expressed protein that is injected into the host within the first 24h of the tick attaching onto the host as revealed by Western blotting analyses of recombinant (r)AamAV422, tick saliva and dissected tick organ protein extracts using antibodies to 24 and 48 h tick saliva proteins. Native AamAV422 is apparently involved with mediating tick anti-hemostasis and anti-complement functions in that rAamAV422 delayed plasma clotting time in a dose responsive manner by up to ≈ 160 s, prevented platelet aggregation by up to ≈ 16% and caused ≈ 24% reduction in production of terminal complement complexes. Target validation analysis revealed that rAamAV422 is a potential candidate for a cocktail or multivalent tick vaccine preparation in that RNA interference (RNAi)-mediated silencing of AamAV422 mRNA caused a statistically significant (≈ 44%) reduction in tick engorgement weights, which is proxy for amounts of ingested blood. We speculate that AamAV422 is a potential target antigen for development of the highly desired universal tick vaccine in that consistent with high conservation among ticks, antibodies to 24h Ixodes scapularis tick saliva proteins specifically bound rAamAV422. We discuss data in this study in the context of advancing the biology of tick feeding physiology and discovery of potential target antigens for tick vaccine development.
authors: Texas A & M University AgriLife Research, Department of Entomology, 2475 TAMU, College Station, TX 77843, USA. a-mulenga@tamu.edu
authors: Albert
authors: A
authors: Mulenga
authors: Tae Kwon
authors: TK
authors: Kim
authors: Adriana Mércia Guaratini
authors: AM
authors: Ibelli
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D003165:Complement System Proteins / Q000378:metabolism; D017124:Conserved Sequence; D005247:Feeding Behavior; D026863:Ixodidae / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D012471:Salivary Proteins and Peptides; D003165:Complement System Proteins