title: Antidiabetic effect of Streblus asper in streptozotocin-induced diabetic rats.
abstract: BACKGROUND
  In the Indian traditional system of medicine, Streblus asper Lour (Moraceae) is prescribed for the treatment of diabetes mellitus.
  
  
  OBJECTIVE
  In the present study, α-amyrin acetate isolated from S. asper, and the petroleum ether extract of S. asper stem bark (PESA) was screened for their antidiabetic properties in streptozotocin (STZ)-induced diabetic rats.
  
  
  METHODS
  Successive Soxhlet extraction of the dried stem bark with petroleum ether and then with ethanol (95%) yielded petroleum ether and ethanol extracts, respectively, which were concentrated under reduced pressure. Hyperglycemia was induced in rats by STZ (50 mg/kg, b.w.). Twenty-four hours after STZ induction, respective groups of diabetic rats received PESA (100, 250 and 500 mg/kg, b.w.) and α-amyrin acetate (25, 50 and 75 mg/kg, b.w.) respectively, orally daily for 15 days. Glibenclamide (0.5 mg/kg, orally) served as a reference. Blood glucose levels were measured on every 5th day during the 15 days of treatment. The serum lipid profiles and biochemical parameters, viz., serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), insulin and glycosylated hemoglobin level, were measured.
  
  
  RESULTS
  PESA significantly (p < 0.01) normalized blood-glucose levels and serum biochemical parameters as compared with those of STZ controls. α-Amyrin acetate (75 mg/kg, b.w.) exhibited maximum glucose lowering effect (71.10%) in diabetic rats compared to the other dose (25, 50 mg/kg) at the end of the study. The protective effect was further confirmed by histopathological examination of the liver.
  
  
  CONCLUSIONS
  PESA and α-amyrin acetate demonstrated remarkable antidiabetic activity in STZ-induced diabetic rats.
authors: Department of Pharmaceutical Chemistry, Seemanta Institute of Pharmaceutical Sciences, Jharpokharia, Odisha, India. sanjay_karan21@rediffmail.com
authors: Sanjay Kumar
authors: SK
authors: Karan
authors: Arijit
authors: A
authors: Mondal
authors: Sagar Kumar
authors: SK
authors: Mishra
authors: Dilipkumar
authors: D
authors: Pal
authors: Kedar Kumar
authors: KK
authors: Rout
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry / Q000627:therapeutic use; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D020834:Ethnopharmacology; D006943:Hyperglycemia / Q000517:prevention & control; D006949:Hyperlipidemias / Q000150:complications / Q000517:prevention & control; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry / Q000627:therapeutic use*; D007194:India; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D029586:Moraceae / Q000737:chemistry*; D020556:Neutrophil Infiltration / Q000187:drug effects; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry / Q000627:therapeutic use*; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D013311:Streptozocin; D023382:Toxicity Tests, Acute
chemical_list: D000975:Antioxidants; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D013311:Streptozocin; D009828:Oleanolic Acid; C000265:beta-amyrin acetate