title: Cross-linked tyrosinase aggregates for elimination of phenolic compounds from wastewater.
abstract: A novel, practical and inexpensive immobilization method has been developed for mushroom tyrosinase to be used for enzymatic treatment of phenolic wastewater. Catalyzed by the enzyme immobilized in the form of cross-linked enzyme aggregates (CLEAs), phenolic compounds such as phenol, p-cresol, p-chlorophenol and bisphenol A can be efficiently eliminated, with a complete conversion obtained within 0.5-3h, superior to other processes catalyzed by the same enzyme which is either free or immobilized with traditional carrier-bound immobilization methods. The effects of reaction time, pH, enzyme dosage and initial concentration of the phenol solution were examined. The sequence of dephenolization rate (p-cresol>p-chlorophenol>phenol) was in accordance with the substrate selectivity of the enzyme. The reusability of the CLEAs has been tested in a batch reactor for each phenol. In a continuous stirred tank reactor, the CLEAs encapsulated into calcium alginate gels were effective for removing phenol for at least 26h. The toxicity of the phenol-containing solution was remarkably diminished after treatment with the tyrosinase CLEAs, as demonstrated by the Hydra sinensis test.
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China.
authors: Da-You
authors: DY
authors: Xu
authors: Zhen
authors: Z
authors: Yang
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000464:Alginates / Q000737:chemistry; D000818:Animals; D055162:Biocatalysis; D052918:Environmental Restoration and Remediation; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006829:Hydra / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D013696:Temperature; D013997:Time Factors; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D014442:Monophenol Monooxygenase; D010636:Phenols; D062065:Wastewater