title: A sensitive liquid chromatographic-mass spectrometric method for simultaneous quantification of six iridoid glycosides from Zhi-zi-chi Decoction in rat plasma and its application to a pharmacokinetic study.
abstract: A sensitive liquid chromatographic-mass spectrometric (LC-MS) method was developed and validated for simultaneous determination of geniposide, geniposidic acid, scandoside methyl ester, gardenoside, deacetyl asperulosidic acid methyl ester and genipin-1-β-gentiobioside after oral administration of Zhi-zi-chi Decoction in rat plasma. The six iridoid glycosides were extracted from plasma samples by protein precipitation, and then separated on an Apollo C18 column (250 mm × 4.6mm, 5 μm) through the application of a gradient elution. The analytes were monitored in positive electrospray ionization by selected ion monitoring mode (SIM). The lower limits of quantitation (LLOQ) of the six analytes were all lower than 6 ng/mL. The accuracy (relative error, RE%) was between -7.0% and 9.9%, while the intra- and inter-day precisions (relative standard deviation, RSD%) were less than 6.3% and 9.8% for the six analytes, respectively. The developed method was successfully applied to a comparative pharmacokinetic study of the six iridoids in rat plasma after oral administration of Zhi-zi-chi Decoction and Gardenia jasminoides extract.
authors: The Engineering Laboratory of National and Local Union of Quality Control for Traditional Chinese Medicine, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors: Kankan
authors: K
authors: Qu
authors: Jinna
authors: J
authors: Dai
authors: Longshan
authors: L
authors: Zhao
authors: Yanan
authors: Y
authors: Lu
authors: Bin
authors: B
authors: Li
authors: Xu
authors: X
authors: Zhao
authors: Pengyi
authors: P
authors: Hou
authors: Yuanting
authors: Y
authors: Zhang
authors: Kaishun
authors: K
authors: Bi
authors: Xiaohui
authors: X
authors: Chen
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057889:Iridoid Glycosides / Q000097:blood* / Q000493:pharmacokinetics; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats
chemical_list: D057889:Iridoid Glycosides