title: Evolution of mir-92a underlies natural morphological variation in Drosophila melanogaster.
abstract: Identifying the genetic mechanisms underlying phenotypic change is essential to understanding how gene regulatory networks and ultimately the genotype-to-phenotype map evolve. It is recognized that microRNAs (miRNAs) have the potential to facilitate evolutionary change [1-3]; however, there are no known examples of natural morphological variation caused by evolutionary changes in miRNA expression. Therefore, the contribution of miRNAs to evolutionary change remains unknown [1, 4]. Drosophila melanogaster subgroup species display a portion of trichome-free cuticle on the femur of the second leg called the "naked valley." It was previously shown that Ultrabithorax (Ubx) is involved in naked valley variation between D. melanogaster and D. simulans [5, 6]. However, naked valley size also varies among populations of D. melanogaster, ranging from 1,000 up to 30,000 μm(2). We investigated the genetic basis of intraspecific differences in the naked valley in D. melanogaster and found that neither Ubx nor shavenbaby (svb) [7, 8] contributes to this morphological difference. Instead, we show that changes in mir-92a expression underlie the evolution of naked valley size in D. melanogaster through repression of shavenoid (sha) [9]. Therefore, our results reveal a novel mechanism for morphological evolution and suggest that modulation of the expression of miRNAs potentially plays a prominent role in generating organismal diversity.
authors: Department of Biological and Medical Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, UK.
authors: Saad
authors: S
authors: Arif
authors: Sophie
authors: S
authors: Murat
authors: Isabel
authors: I
authors: Almudi
authors: Maria D S
authors: MD
authors: Nunes
authors: Diane
authors: D
authors: Bortolamiol-Becet
authors: Naomi S
authors: NS
authors: McGregor
authors: James M S
authors: JM
authors: Currie
authors: Harri
authors: H
authors: Hughes
authors: Matthew
authors: M
authors: Ronshaugen
authors: Élio
authors: É
authors: Sucena
authors: Eric C
authors: EC
authors: Lai
authors: Christian
authors: C
authors: Schlötterer
authors: Alistair P
authors: AP
authors: McGregor
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019143:Evolution, Molecular; D005121:Extremities / Q000033:anatomy & histology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011679:Pupa / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D040641:Quantitative Trait Loci
chemical_list: C576274:MIRN92 microRNA, Drosophila; D035683:MicroRNAs