title: Ion-pairing reverse-phase high performance liquid chromatography method for simultaneous estimation of atenolol and indapamide in bulk and combined dosage form.
abstract: A sensitive, accurate, precise and validated ion-pairing reverse-phase liquid chromatographic method for the quantitative determination of atenolol and indapamide in bulk and tablet dosage form was developed. The proposed ion-pairing reverse-phase high performance liquid chromatography method utilises C(18) column with 5 μm, 150×4.6 mm i.d. column and mobile phase consisting of 0.1% w/v solution of octane sulphonic acid, sodium salt and methanol (55:45 v/v), (pH 2.8) and ultraviolet detection at 235 nm. A linearity range of 1-250 μg/ml and 1-25 μg/ml for atenolol and indapamide, respectively, was obtained. The mean recoveries are 100.48 and 99.82% for atenolol and indapamide, respectively. The method was validated as per International Conference on Harmonization guidelines.
authors: Department of Pharmaceutical Chemistry, Y. B. Chavan College of Pharmacy, Rauza Baugh, Aurangabad-431 001, India.
authors: K G
authors: KG
authors: Baheti
authors: N
authors: N
authors: Shah
authors: S
authors: S
authors: Shaikh
keywords: Atenolol; indapamide; ion-pairing; reverse-phase high performance liquid chromatography