title: Deciphering complement receptor type 1 interactions with recognition proteins of the lectin complement pathway.
abstract: Complement receptor type 1 (CR1) is a membrane receptor expressed on a wide range of cells. It is involved in immune complex clearance, phagocytosis, and complement regulation. Its ectodomain is composed of 30 complement control protein (CCP) modules, organized into four long homologous repeats (A-D). In addition to its main ligands C3b and C4b, CR1 was reported to interact with C1q and mannan-binding lectin (MBL) likely through its C-terminal region (CCP22-30). To decipher the interaction of human CR1 with the recognition proteins of the lectin complement pathway, a recombinant fragment encompassing CCP22-30 was expressed in eukaryotic cells, and its interaction with human MBL and ficolins was investigated using surface plasmon resonance spectroscopy. MBL and L-ficolin were shown to interact with immobilized soluble CR1 and CR1 CCP22-30 with apparent dissociation constants in the nanomolar range, indicative of high affinity. The binding site for CR1 was located at or near the MBL-associated serine protease (MASP) binding site in the collagen stalks of MBL and L-ficolin, as shown by competition experiments with MASP-3. Accordingly, the mutation of an MBL conserved lysine residue essential for MASP binding (K55) abolished binding to soluble CR1 and CCP22-30. The CR1 binding site for MBL/ficolins was mapped to CCP24-25 of long homologous repeat D using deletion mutants. In conclusion, we show that ficolins are new CR1 ligands and propose that MBL/L-ficolin binding involves major ionic interactions between conserved lysine residues of their collagen stalks and surface exposed acidic residues located in CR1 CCP24 and/or CCP25.
authors: Commissariat à l'Energie Atomique, Institut de Biologie Structurale Jean-Pierre Ebel, 38027 Grenoble Cedex 1, France.
authors: Mickaël
authors: M
authors: Jacquet
authors: Monique
authors: M
authors: Lacroix
authors: Sarah
authors: S
authors: Ancelet
authors: Evelyne
authors: E
authors: Gout
authors: Christine
authors: C
authors: Gaboriaud
authors: Nicole M
authors: NM
authors: Thielens
authors: Véronique
authors: V
authors: Rossi
mesh_terms: D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism; D037582:Complement Pathway, Mannose-Binding Lectin / Q000235:genetics; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D011951:Receptors, Complement / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000097604:Ficolins
chemical_list: D002352:Carrier Proteins; D037102:Lectins; D037601:Mannose-Binding Lectin; D050606:Mannose-Binding Protein-Associated Serine Proteases; D011951:Receptors, Complement; D011994:Recombinant Proteins; D000097604:Ficolins