title: Canonical transient receptor potential channel subtype 3-mediated hair cell Ca(2+) entry regulates sound transduction and auditory neurotransmission.
abstract: The physiological significance of canonical transient receptor potential (TRPC) ion channels in sensory systems is rapidly emerging. Heterologous expression studies show that TRPC3 is a significant Ca(2+) entry pathway, with dual activation via G protein-coupled receptor (GPCR)-phospholipase C-diacylglycerol second messenger signaling, and through negative feedback, whereby a fall in cytosolic Ca(2+) releases Ca(2+) -calmodulin channel block. We hypothesised that the latter process contributes to cochlear hair cell cytosolic Ca(2+) homeostasis. Confocal microfluorimetry with the Ca(2+) indicator Fluo-4 acetoxymethylester showed that, when cytosolic Ca(2+) was depleted, Ca(2+) re-entry was significantly impaired in mature TRPC3(-/-) inner and outer hair cells. The impact of this disrupted Ca(2+) homeostasis on sound transduction was assessed with the use of distortion product otoacoustic emissions (DPOAEs), which constitute a direct measure of the outer hair cell transduction that underlies hearing sensitivity and frequency selectivity. TRPC3(-/-) mice showed significantly stronger DPOAE (2f1  - f2 ) growth functions than wild-type (WT) littermates within the frequency range of best hearing acuity. This translated to hyperacusis (decreased threshold) measured by the auditory brainstem response (ABR). TRPC3(-/-) and WT mice did not differ in the levels of temporary and permanent threshold shift arising from noise exposure, indicating that potential GPCR signaling via TRPC3 is not pronounced. Overall, these data suggest that the Ca(2+) set-point in the hair cell, and hence membrane conductance, is modulated by TRPC3s through their function as a negative feedback-regulated Ca(2+) entry pathway. This TPRC3-regulated Ca(2+) homeostasis shapes the sound transduction input-output function and auditory neurotransmission.
authors: Translational Neuroscience Facility and Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney 2052, NSW, Australia.
authors: Ann Chi Yan
authors: AC
authors: Wong
authors: Lutz
authors: L
authors: Birnbaumer
authors: Gary D
authors: GD
authors: Housley
mesh_terms: D000200:Action Potentials; D000818:Animals; D001306:Auditory Pathways / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D001933:Brain Stem / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D003600:Cytosol / Q000378:metabolism; D025461:Feedback, Physiological; D006198:Hair Cells, Auditory / Q000378:metabolism* / Q000502:physiology; D006309:Hearing; D006706:Homeostasis; D051379:Mice; D008822:Mice, Transgenic; D012684:Sensory Thresholds; D015398:Signal Transduction; D013016:Sound; D009435:Synaptic Transmission; D050052:TRPC Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D002118:Calcium