title: Composition analysis and immuno-modulatory effect of okra (Abelmoschus esculentus L.) extract.
abstract: The aim of this study was to analyse the composition of okra (Abelmoschus esculentus L.) extract and investigate the effect of A. esculentus L. polysaccharides (AE-PS) on the maturation and function of dendritic cells (DCs) derived from rat bone marrow hematopoietic cells (BMHCs) in vitro. BMHC-derived immature DCs (BMHC-imDCs) were extracted from rats and treated with AE-PS. The hydrolysed okra extract contained 0.6% β-1, 3-D-glucan. AE-PS induced the presence of polymorphic nuclei and elongated protrusion in the BHMC-imDCs, indicating DC activation. Treatment with 100 μg/mL of AE-PS increased the MHC class II and CD80/86 expression levels by 41% and 42%, respectively. Treated cells had reduced endocytosis activity. The secretion of IL-12 and IFN-γ increased significantly by 120% and 75%, respectively, when treated with 100 μg/mL of AE-PS. Moreover, IL-10 production was reduced by 66%. In conclusion, AE-PS exhibits stimulatory effects on rat dendritic cells and promotes the secretion of T(H)1 cytokines.
authors: Department of Food Science, National Pingtung University of Science and Technology, No. 1 Shuehfu Rd., Neipu, Pingtung 91201, Taiwan, ROC. ssheu@mail.npust.edu.tw
authors: Shyang-Chwen
authors: SC
authors: Sheu
authors: Mei-Huei
authors: MH
authors: Lai
mesh_terms: D028483:Abelmoschus / Q000737:chemistry*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D016753:Interleukin-10 / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides; D016753:Interleukin-10; D018664:Interleukin-12