title: Dissemination of blaVIM in Greece at the peak of the epidemic of 2005-2006: clonal expansion of Klebsiella pneumoniae clonal complex 147.
abstract: VIM-producing Klebsiella pneumoniae (n = 21) isolated from ten Greek hospitals during 2003-2007 were characterized with multilocus sequence typing (MLST), semi-automated repetitive sequence-based PCR (rep-PCR) (Diversilab), plasmid replicon typing, serotyping and screening for multiple resistance determinants. The isolates were selected to represent different strain clusters (defined by >80% similarity) according to pulsed-field gel electrophoresis. MLST identified three major clonal complexes (CCs); CC147 (n = 8), CC18 (n = 5) and CC14 (n = 3). Plasmid replicon typing showed that IncA/C and/or IncFIIK replicons were detected among isolates in each of the major CCs. Good concordance was observed between semi-automated-rep PCR genotyping and MLST.
authors: Clinical Microbiology L2:02, Karolinska Institutet, MTC, Karolinska University Hospital, Stockholm, Sweden.
authors: C M
authors: CM
authors: Hasan
authors: A
authors: A
authors: Turlej-Rogacka
authors: A C
authors: AC
authors: Vatopoulos
authors: P
authors: P
authors: Giakkoupi
authors: M
authors: M
authors: Maâtallah
authors: C G
authors: CG
authors: Giske
mesh_terms: D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D060005:Genotyping Techniques; D006115:Greece / Q000453:epidemiology; D049674:History, 21st Century; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D058885:Multilocus Sequence Typing; D010957:Plasmids; D012703:Serotyping; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases
keywords: AmpC; MLST; clonal complex; metallo-β-lactamase; plasmid replicon