title: Neurotoxic effects of lambda-cyhalothrin modulated by piperonyl butoxide in the brain of Oreochromis niloticus.
abstract: The objective of this research was to investigate the neurotoxic effects of pyrethroid pesticide lambda-cyhalothrin by the modulation of cytochrome P450 with piperonyl butoxide in the brain of juvenile Oreochromis niloticus. The fish were exposed to 0.48 μg L(-1) (1/6 of the 96-h LC50 ) lambda-cyhalothrin and 10 μg L(-1) piperonyl butoxide for 96 h and 15 days. tGSH, GSSG, TBARS contents, GPx, GR, GST, and AChE enzymes activities were determined by spectrophotometrical methods and Hsp70 content was analyzed by ELISA technique. Lambda-cyhalothrin had no significant effect on the components of GSH redox system, lipid peroxidation and Hsp70 levels but inhibited AChE activity. In the presence of piperonyl butoxide, lambda-cyhalothrin caused increases in tGSH, GSSG, TBARS and Hsp70 contents, GST activity, and decrease in AChE activity. Present results showed that in the presence of piperonyl butoxide, lambda-cyhalothrin caused neurotoxic effects by increasing oxidative stress. Adaptation to its oxidative stress effects may be supplied by GSH-related antioxidant system. Piperonyl butoxide revealed neurotoxic effect of lambda-cyhalothrin.
authors: Division of Science Education, Faculty of Education, Kahramanmaraş Sütçü İmam University, Avşar Campus, 46100, Kahramanmaraş, Turkey.
authors: Petek
authors: P
authors: Piner
authors: Nevin
authors: N
authors: Üner
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D023681:Cichlids / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004357:Drug Synergism; D005978:Glutathione / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D009570:Nitriles / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity*; D010882:Piperonyl Butoxide / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D009570:Nitriles; D010575:Pesticides; D011722:Pyrethrins; D017392:Thiobarbituric Acid Reactive Substances; D003577:Cytochrome P-450 Enzyme System; D005978:Glutathione; D010882:Piperonyl Butoxide; C037304:cyhalothrin
keywords: Lambda-cyhalothrin; Oreochromis niloticus; brain; oxidative stress; piperonyl butoxide