title: TWEAK enhances E-selectin and ICAM-1 expression, and may contribute to the development of cutaneous vasculitis.
abstract: Our previous work indicated that TWEAK is associated with various types of cutaneous vasculitis (CV). Herein, we investigate the effects of TWEAK on vascular injury and adhesion molecule expression in CV mice. We showed that TWEAK priming in mice induced a local CV. Furthermore, TWEAK priming also increased the extravasation of FITC-BSA, myeloperoxidase activity and the expression of E-selectin and ICAM-1. Conversely, TWEAK blockade ameliorated the LPS-induced vascular damage, leukocyte infiltrates and adhesion molecules expression in LPS-induced CV. In addition, TWEAK treatment of HDMECs up-regulated E-selectin and ICAM-1 expression at both mRNA and protein levels. TWEAK also enhanced the adhesion of PMNs to HDMECs. Finally, western blot data revealed that TWEAK can induce phosphorylation of p38, JNK and ERK in HDMECs. These data suggest that TWEAK acted as an inducer of E-selectin and ICAM-1 expression in CV mice and HDMECs, may contribute to the development of CV.
authors: Department of Dermatovenereology, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
authors: Tao
authors: T
authors: Chen
authors: Zai-pei
authors: ZP
authors: Guo
authors: Li
authors: L
authors: Li
authors: Meng-meng
authors: MM
authors: Li
authors: Ting-ting
authors: TT
authors: Wang
authors: Rui-zhen
authors: RZ
authors: Jia
authors: Na
authors: N
authors: Cao
authors: Jing-yi
authors: JY
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D000074049:Cytokine TWEAK; D019040:E-Selectin / Q000235:genetics*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood; D000074050:TWEAK Receptor; D048069:Tumor Necrosis Factors / Q000097:blood / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects; D018366:Vasculitis, Leukocytoclastic, Cutaneous / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: D000074049:Cytokine TWEAK; D019040:E-Selectin; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D018124:Receptors, Tumor Necrosis Factor; D000074050:TWEAK Receptor; C491645:Tnfsf12 protein, mouse; D048069:Tumor Necrosis Factors; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase