title: [Protective effect of hydrogen against hyperoxia-induced type II alveolar epithelial cell injury].
abstract: OBJECTIVE
  To investigate the protective effect of hydrogen against hyperoxia-induced oxidative stress injury in premature rat type II alveolar epithelial cells (AECs).
  
  
  METHODS
  The type II AECs isolated from premature rats were randomly divided into air (21% oxygen) control group, hyperoxia (95% oxygen) control group, air + hydrogen group, and hyperoxia+ hydrogen group. The cells with hydrogen treatment were cultured in the presence of rich hydrogen. After the corresponding exposure for 24 h, the cell morphology was observed microscopically. MTT assay was used to evaluated the cell proliferation ability, and JC-1 fluorescence probe was used to detect the mitochondrial membrane potential (δφ) changes of the type II AECs. The concentration of maleic dialdehyde (MDA) and superoxide dismutase (SOD) activity in the cell supernatant were detected using colorimetric method.
  
  
  RESULTS
  No significant differences were found in cell growth or measurements between air control and air + hydrogen groups. Compared with air control group, the cells exposed to hyperoxia showed significantly suppressed proliferation, reduced mitochondrial membrane potential, increased MDA content, and decreased SOD activity.  Intervention with hydrogen resulted in significantly increased cell proliferation and SOD activity and lowered MDA content, and restored the mitochondrial membrane potential in the cells with hyperoxia exposure (P<0.05).
  
  
  CONCLUSIONS
  Hydrogen can significantly reduce hyperoxia-induced oxidative stress injury in premature rat type II AECs, improve the cellular antioxidant capacity, stabilize the mitochondrial membrane potential, and reduce the inhibitory effect of hyperoxia on cell proliferation.
authors: Children's Hospital Affiliated to Chongqing Medical University, Chongqing, China. yaolankaoyan2008@163.com
authors: Lan
authors: L
authors: Yao
authors: Feng
authors: F
authors: Xu
authors: Chong
authors: C
authors: Luo
authors: Pan
authors: P
authors: Yu
authors: Xinxin
authors: X
authors: Dong
authors: Xuejun
authors: X
authors: Sun
authors: Chengjun
authors: C
authors: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D005260:Female; D006859:Hydrogen / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000009:adverse effects*; D011650:Pulmonary Alveoli / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D006859:Hydrogen; D013482:Superoxide Dismutase; D010100:Oxygen