title: Expression and regulation of miR-17a and miR-430b in zebrafish ovarian follicles.
abstract: MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression and control many developmental and physiological processes. Oocyte maturation in fish is mainly regulated by luteinizing hormone (LH) and maturation-inducing hormone (MIH). In addition, growth factors, including members of the transforming growth factor β (TGF-β) superfamily, have also been shown to play important roles in regulating oocyte maturation. In this study, we determined the expression and regulation of two miRNAs, miR-17a and miR-430b, which potentially target signalling molecules in the TGF-β pathway, in zebrafish ovarian follicles. Using real-time PCR, we observed that miR-17a and miR-430b levels in follicular cells were significantly lower in late vitellogenic and full grown follicles than in early vitellogenic follicles. Treatment with a LH analog, human chorionic gonadotropin, significantly down-regulated miR-17a and miR-430b expression in follicular cells but had no effect on their expression in oocytes. Forskolin also inhibited follicular cell miR-430b expression; however, no significant changes in miR-17a levels were observed after Forskolin treatment. Finally, MIH did not affect the expression of these miRNAs either in follicular cells or oocytes at the time points tested. These findings suggest that miR-17a and miR-430b may be involved in the regulation of follicle development and oocyte maturation in zebrafish.
authors: Department of Biology, York University, Toronto, Canada.
authors: Rina
authors: R
authors: Abramov
authors: Guodong
authors: G
authors: Fu
authors: Yong
authors: Y
authors: Zhang
authors: Chun
authors: C
authors: Peng
mesh_terms: D000818:Animals; D005260:Female; D006062:Gonadotropins / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*
chemical_list: D006062:Gonadotropins; D035683:MicroRNAs; C557527:Mirn430 microRNA, zebrafish