title: Reduced expression of ZDHHC2 is associated with lymph node metastasis and poor prognosis in gastric adenocarcinoma.
abstract: BACKGROUND
  Zinc finger, DHHC-type containing 2 (ZDHHC2), originally named as reduced expression associated with metastasis protein (REAM), has been proposed as a putative tumor/metastasis suppressor gene and is often aberrantly decreased in human cancers. However ZDHHC2 expression pattern and its clinical significance have not yet been investigated in gastric adenocarcinoma.
  
  
  RESULTS
  Quantitative Real-Time PCR (qRT-PCR) and immunostaining were performed to detect ZDHHC2 expression in gastric adenocarcinoma, and then the correlation between ZDHHC2 expression and clinicpathologic parameters, and patient survival was analyzed. Compared to the adjacent normal tissues, ZDHHC2 expression was significantly reduced in gastric tumor tissues as shown by qRT-PCR and immunostaining. Low expression of ZDHHC2 was observed in 44.7% (211/472) of gastric adenocarcinoma patients, and was associated significantly with lymph node metastasis (p<0.001) and histological grade (p<0.001). Multivariate Cox regression analysis indicated that ZDHHC2 expression had a significant, independent predictive value for survival of gastric cancer patients (HR = 0.627, p = 0.001).
  
  
  CONCLUSIONS
  Our data suggest that reduced ZDHHC2 expression is associated with lymph node metastasis and independently predicts an unfavorable prognosis in gastric adenocarcinoma patients.
authors: State Key Laboratory of Oncology in South China and Department of Pathology, Sun Yat-Sen University Cancer Center, Guangzhou, PR China.
authors: Shu-Mei
authors: SM
authors: Yan
authors: Jian-Jun
authors: JJ
authors: Tang
authors: Chun-Yu
authors: CY
authors: Huang
authors: Shao-Yan
authors: SY
authors: Xi
authors: Ma-Yan
authors: MY
authors: Huang
authors: Jian-Zhong
authors: JZ
authors: Liang
authors: Yuan-Xue
authors: YX
authors: Jiang
authors: Yu-Hong
authors: YH
authors: Li
authors: Zhi-Wei
authors: ZW
authors: Zhou
authors: Ingemar
authors: I
authors: Ernberg
authors: Qiu-Liang
authors: QL
authors: Wu
authors: Zi-Ming
authors: ZM
authors: Du
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016019:Survival Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D025521:Tumor Suppressor Proteins; D000217:Acyltransferases; C532036:ZDHHC2 protein, human