title: Fermentation approach for enhancing 1-butanol production using engineered butanologenic Escherichia coli.
abstract: In this study, engineered butanologenic Escherichia coli T5 constructed by the OGAB method was used for 1-butanol production. The results showed the feasibility of the artificial butanologenic operon, (Promoter Pr)-thil-crt-bcd-etfB-etfA-hbd-adhe1-adhe, where the 1-butanol titer, specific BuOH yield, and BuOH yield were 4.50 mg/L, 4.50 mg-BuOH/g cell, and 0.35 mg-BuOH/g-glucose, respectively. Fermentation conditions of anaerobic, low initial concentrations of carbon sources, low oxidation state of carbon source, pH of 6, addition of glutathione and citrate, had been shown for efficiently improving the 1-butanol production. The premise behind these fermentation approaches can be categorized into two lines of reasoning, either elevated the availability of acetyl-CoA or lowered the intracellular redox state. By comparing the fermentation conditions tested in this study, pH has been shown to be the most efficiency strategies for 1-butanol production while the replacement of glucose with glycerol provides the highest improvement in butanol yield.
authors: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors: Shang-Kai
authors: SK
authors: Chen
authors: Wei-Chih
authors: WC
authors: Chin
authors: Kenji
authors: K
authors: Tsuge
authors: Chieh-Chen
authors: CC
authors: Huang
authors: Si-Yu
authors: SY
authors: Li
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000693:Anaerobiosis; D057005:Bioengineering / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D000105:Acetyl Coenzyme A; D020001:1-Butanol; D005990:Glycerol
keywords: 1-Butanol; E. coli; Fermentation approach; OGAB