title: Biobutanol production from rice straw by a non acetone producing Clostridium sporogenes BE01.
abstract: Biobutanol from lignocellulosic biomass has gained much attention due to several advantages over bioethanol. Though microbial production of butanol through ABE fermentation is an established technology, the use of lignocellulosic biomass as feedstock presents several challenges. In the present study, biobutanol production from enzymatic hydrolysate of acid pretreated rice straw was evaluated using Clostridium sporogenes BE01. This strain gave a butanol yield of 3.43 g/l and a total solvent yield of 5.32 g/l in rice straw hydrolysate supplemented with calcium carbonate and yeast extract. Hydrolysate was analyzed for the level of inhibitors such as acetic acid, formic acid and furfurals which affect the growth of the organism and in turn ABE fermentation. Methods for preconditioning the hydrolysate to remove toxic end products were done so as to improve the fermentation efficiency. Conditions of ABE fermentation were fine tuned resulting in an enhanced biobutanol reaching 5.52 g/l.
authors: Centre for Biofuels, Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate PO, Thiruvananthapuram, India.
authors: Lalitha Devi
authors: LD
authors: Gottumukkala
authors: Binod
authors: B
authors: Parameswaran
authors: Sajna Kuttavan
authors: SK
authors: Valappil
authors: Kuttiraja
authors: K
authors: Mathiyazhakan
authors: Ashok
authors: A
authors: Pandey
authors: Rajeev Kumar
authors: RK
authors: Sukumaran
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*
chemical_list: D056804:Biofuels; D000440:Butanols; C036909:lignocellulose; D008031:Lignin
keywords: ABE fermentation; Biobutanol; Clostridium sporogenes; Detoxification; Rice straw hydrolysate