title: Mathematical modeling of poly[(R)-3-hydroxyalkanoate] synthesis by Cupriavidus necator DSM 545 on substrates stemming from biodiesel production.
abstract: Two low structured mathematical models for fed-batch production of polyhydroxybutyrate and poly[hydroxybutyrate-co-hydroxyvalerate] by Cupriavidus necator DSM 545 on renewable substrates (glycerol and fatty acid methyl esters-FAME) combined with glucose and valeric acid, were established. The models were used for development/optimization of feeding strategies of carbon and nitrogen sources concerning PHA content and polymer/copolymer composition. Glycerol/glucose fermentation featured a max. specific growth rate of 0.171 h(-1), a max. specific production rate of 0.038 h(-1) and a PHB content of 64.5%, whereas the FAME/valeric acid fermentation resulted in a max. specific growth rate of 0.046 h(-1), a max. specific production rate of 0.07 h(-1) and 63.6% PHBV content with 4.3% of 3-hydroxyvalerate (3HV) in PHBV. A strong inhibition of glycerol consumption by glucose was confirmed (inhibition constant ki,G=4.28×10(-4) g L(-1)). Applied concentration of FAME (10-12 g L(-1)) positively influenced on PHBV synthesis. HV/PHBV ratio depends on applied VA concentration.
authors: Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
authors: Ivna Vrana
authors: IV
authors: Špoljarić
authors: Markan
authors: M
authors: Lopar
authors: Martin
authors: M
authors: Koller
authors: Alexander
authors: A
authors: Muhr
authors: Anna
authors: A
authors: Salerno
authors: Angelika
authors: A
authors: Reiterer
authors: Karin
authors: K
authors: Malli
authors: Hannes
authors: H
authors: Angerer
authors: Katharina
authors: K
authors: Strohmeier
authors: Sigurd
authors: S
authors: Schober
authors: Martin
authors: M
authors: Mittelbach
authors: Predrag
authors: P
authors: Horvat
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000382:microbiology*; D003198:Computer Simulation; D020118:Cupriavidus necator / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004952:Esters / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007700:Kinetics; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009584:Nitrogen / Q000494:pharmacology; D010421:Pentanoic Acids / Q000494:pharmacology; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D015203:Reproducibility of Results
chemical_list: D056804:Biofuels; D004952:Esters; D010421:Pentanoic Acids; D054813:Polyhydroxyalkanoates; C038780:n-pentanoic acid; D005947:Glucose; D009584:Nitrogen; D005990:Glycerol