title: H3K4me3 interactions with TAF3 regulate preinitiation complex assembly and selective gene activation.
abstract: Histone modifications regulate chromatin-dependent processes, yet the mechanisms by which they contribute to specific outcomes remain unclear. H3K4me3 is a prominent histone mark that is associated with active genes and promotes transcription through interactions with effector proteins that include initiation factor TFIID. We demonstrate that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that (1) H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex (PIC) formation; (2) H3K4me3, through TAF3 interactions, can act either independently or cooperatively with the TATA box to direct PIC formation and transcription; and (3) H3K4me3-TAF3/TFIID interactions regulate gene-selective functions of p53 in response to genotoxic stress. Our findings indicate a mechanism by which H3K4me3 directs PIC assembly for the rapid induction of specific p53 target genes.
authors: Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA.
authors: Shannon M
authors: SM
authors: Lauberth
authors: Takahiro
authors: T
authors: Nakayama
authors: Xiaolin
authors: X
authors: Wu
authors: Andrea L
authors: AL
authors: Ferris
authors: Zhanyun
authors: Z
authors: Tang
authors: Stephen H
authors: SH
authors: Hughes
authors: Robert G
authors: RG
authors: Roeder
mesh_terms: D045744:Cell Line, Tumor; D042421:Histone Code; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D016385:TATA Box; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID / Q000378:metabolism*; D061785:Transcription Initiation, Genetic; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D050880:p300-CBP Transcription Factors / Q000378:metabolism
chemical_list: D006657:Histones; C432689:TAF3 protein, human; D035381:TATA-Binding Protein Associated Factors; C495901:TP53 protein, human; D035362:Transcription Factor TFIID; D016159:Tumor Suppressor Protein p53; D050880:p300-CBP Transcription Factors; D008239:Lysine