title: Topical treatment of the buccal mucosa and wounded skin in rats with a triamcinolone acetonide-loaded hydrogel prepared using an electron beam.
abstract: In this study, a triamcinolone acetonide-loaded hydrogel was prepared by electron beam irradiation and evaluated for use as a buccal mucoadhesive drug delivery system. A poloxamer was modified to have vinyl end groups for preparation of the hydrogel via an irradiation cross-linking reaction. Carbopol was introduced to improve the mucoadhesive properties of the hydrogel. The in vitro release of triamcinolone acetonide from the hydrogel was examined at 37 °C. To investigate the topical therapeutic effect of triamcinolone acetonide on wounded rat skin and buccal mucosa, the appearance and histological changes were evaluated for 15 days after treatment with saline, triamcinolone acetonide solution, triamcinolone acetonide hydrogel, and blank hydrogel, respectively. Triamcinolone acetonide was released constantly from the gel formulation at 37 °C and reach 100% at about 48 h. After 15 days, in the skin of the group treated with the triamcinolone acetonide-loaded hydrogel, the wound was almost completely free of crust and a number of skin appendages, including hair follicles, had formed at the margins of the tissue. Moreover, the inflammatory response in the buccal mucosa was milder than that in the other groups, and the wound surface was completely covered with regenerating, hyperkeratotic, thickened epithelial cells. Our results indicate that the triamcinolone-acetonide hydrogel showed sustained drug release behavior, while causing no significant histopathological changes in buccal and skin tissues. Therefore, this hydrogel system may be a powerful means of drug delivery for buccal administration with controlled release and no tissue irritation.
authors: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Soon Gil
authors: SG
authors: Choi
authors: Eun Jung
authors: EJ
authors: Baek
authors: Enkhzaya
authors: E
authors: Davaa
authors: Young-Chang
authors: YC
authors: Nho
authors: Youn-Mook
authors: YM
authors: Lim
authors: Jong-Seok
authors: JS
authors: Park
authors: Hui-Jeong
authors: HJ
authors: Gwon
authors: Kang Moo
authors: KM
authors: Huh
authors: Jeong-Sook
authors: JS
authors: Park
mesh_terms: D000278:Administration, Buccal; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D004583:Electrons; D020100:Hydrogels; D009061:Mouth Mucosa / Q000033:anatomy & histology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000033:anatomy & histology / Q000187:drug effects* / Q000293:injuries; D014222:Triamcinolone Acetonide / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D020100:Hydrogels; D014222:Triamcinolone Acetonide