title: Macrophages are involved in the protective role of human umbilical cord-derived stromal cells in renal ischemia-reperfusion injury.
abstract: Administration of fibroblastic cells derived from a number of tissues (collectively called "mesenchymal stem cells") has been suggested to be beneficial for renal repair and mortality reduction in renal ischemia-reperfusion injury (IRI), but the underlying mechanism is not fully understood. In the present study, our objective was to investigate the involvement of macrophages in the therapeutic effect of human umbilical cord-derived stromal cells (hUCSCs) on renal IRI. Twenty-four hours after reperfusion, hUCSCs were injected intravenously and resulted in significant improvements in renal function, with a lower tubular injury score together with more proliferative and fewer apoptotic tubular cells in kidney tissue. Moreover, hUCSCs reduced the infiltration of macrophages into renal interstitium especially at 5 days post-reperfusion, while the proportion of anti-inflammatory M2 macrophages was markedly increased. HUCSCs also alleviated the local inflammatory response in kidneys. The absence of macrophages during the early phase of reperfusion enhanced the therapeutic effect of hUCSCs, whereas macrophage depletion during the late repair phase eliminated the renoprotective role of hUCSCs. In vitro, macrophages cocultured with hUCSCs were switched to the alternatively activated M2 phenotype. Our data indicate that hUCSCs are capable of promoting the M2 polarization of macrophages at injury sites, suggesting a new mechanism for hUCSC-mediated protection in renal IRI.
authors: School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors: Wei
authors: W
authors: Li
authors: Qiang
authors: Q
authors: Zhang
authors: Mei
authors: M
authors: Wang
authors: Huiyi
authors: H
authors: Wu
authors: Fei
authors: F
authors: Mao
authors: Bin
authors: B
authors: Zhang
authors: Runbi
authors: R
authors: Ji
authors: Shuo
authors: S
authors: Gao
authors: Zixuan
authors: Z
authors: Sun
authors: Wei
authors: W
authors: Zhu
authors: Hui
authors: H
authors: Qian
authors: Yongchang
authors: Y
authors: Chen
authors: Wenrong
authors: W
authors: Xu
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D058948:Cell Tracking; D018920:Coculture Techniques; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D015427:Reperfusion Injury / Q000628:therapy*; D017154:Stromal Cells / Q000166:cytology* / Q000637:transplantation; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D016207:Cytokines