title: Proteome analysis of the two-component SalK/SalR system in Epidemic Streptococcus suis serotype 2.
abstract: Streptococcus suis serotype 2 (SS2) is a major swine pathogen of significant commercial importance worldwide and an emerging zoonotic agent of human streptococcal toxic shock-like syndrome. We previously reported that the Chinese highly pathogenic SS2 strains specifically harbor an 89K pathogenicity island, and the SalK/SalR two-component system encoded within this island mediates a piglet-lethal phenotype. However, its regulatory mechanism remains obscure. In the present study, we performed a proteome analysis to identify the target proteins regulated by the SalK/SalR system. This expression profiling analysis reveals 14 down-regulated proteins and 1 up-regulated protein. In partial accord with our previous microarray analysis, 9 out of the 14 down-regulated genes are also repressed at the transcriptional level. From the 9 matched genes, 5 were selected and further subjected to gene knockout and experimental infection of mice. The results show that the selected 5 genes are potential virulence factors and participate to various extents in the pathogenesis of SS2 infection, and may contribute to the attenuation of virulence mediated by the SalK/SalR system.
authors: Department of Microbiology, Third Military Medical University, Chongqing 400038, China.
authors: Xiaodong
authors: X
authors: Shen
authors: Qiu
authors: Q
authors: Zhong
authors: Yan
authors: Y
authors: Zhao
authors: Supeng
authors: S
authors: Yin
authors: Tian
authors: T
authors: Chen
authors: Fuquan
authors: F
authors: Hu
authors: Ming
authors: M
authors: Li
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D044404:Genomic Islands; D051379:Mice; D020543:Proteome / Q000032:analysis*; D018085:Regulon; D013290:Streptococcal Infections / Q000382:microbiology / Q000473:pathology; D017011:Streptococcus suis / Q000737:chemistry* / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D014157:Transcription Factors; D037521:Virulence Factors