title: Antimicrobial activity of fibrinogen and fibrinogen-derived peptides--a novel link between coagulation and innate immunity.
abstract: Fibrinogen is a key player in the blood coagulation system, and is upon activation with thrombin converted into fibrin that subsequently forms a fibrin clot. In the present study, we investigated the role of fibrinogen in the early innate immune response. Here we show that the viability of fibrinogen-binding bacteria is affected in human plasma activated with thrombin. Moreover, we found that the peptide fragment GHR28 released from the β-chain of fibrinogen has antimicrobial activity against bacteria that bind fibrinogen to their surface, whereas non-binding strains are unaffected. Notably, bacterial killing was detected in Group A Streptococcus bacteria entrapped in a fibrin clot, suggesting that fibrinogen and coagulation is involved in the early innate immune system to quickly wall off and neutralise invading pathogens.
authors: Department of Clinical Sciences, Lund, Division of Infection Medicine, Lund University, Tornavägen 10, Lund, Sweden. Lisa.Pahlman@med.lu.se
authors: L I
authors: LI
authors: Påhlman
authors: M
authors: M
authors: Mörgelin
authors: G
authors: G
authors: Kasetty
authors: A I
authors: AI
authors: Olin
authors: A
authors: A
authors: Schmidtchen
authors: H
authors: H
authors: Herwald
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D001777:Blood Coagulation; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D050296:Microbial Viability; D010446:Peptide Fragments / Q000378:metabolism*; D013917:Thrombin / Q000378:metabolism; D013997:Time Factors
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D010446:Peptide Fragments; D005340:Fibrinogen; D013917:Thrombin