title: Reversible binding and quantification of heparin and chondroitin sulfate in water using redox-stable biferrocenylene SAMs.
abstract: A biferrocenylene (BFD) thiol conjugate was prepared and self-assembled on a gold surface. The resulting SAMs with their extraordinary stability in the monocationic state were used to detect and quantify heparin (3-300 × 10(-5) g L(-1) = 0.003-0.3 U mL(-1)) and chondroitin sulfate (3-250 × 10(-5) g L(-1)) in aqueous buffer by cyclic voltammetry, square wave voltammetry and surface plasmon resonance. As a proof of concept, heparin quantification was performed in a blood plasma sample. Moreover, controlled binding and release of heparin was realised by switching the BFD-SAM between the monocationic and neutral state.
authors: Center of Micro- and Nanochemistry and Engineering, Universität Siegen, Organische Chemie I, Adolf-Reichwein-Strasse 2, D-57068 Siegen, Germany.
authors: Kun
authors: K
authors: Chen
authors: Michael
authors: M
authors: Schmittel
mesh_terms: D002809:Chondroitin Sulfates / Q000032:analysis*; D055664:Electrochemical Techniques; D004563:Electrochemistry; D006046:Gold; D006493:Heparin / Q000032:analysis* / Q000097:blood*; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds; D020349:Surface Plasmon Resonance; D014867:Water / Q000032:analysis*
chemical_list: D013438:Sulfhydryl Compounds; D014867:Water; D006046:Gold; D006493:Heparin; D002809:Chondroitin Sulfates