title: Metabolomic changes in follicular fluid induced by soy isoflavones administered to rats from weaning until sexual maturity.
abstract: Female Wistar rats at 21 days of age were treated with one of three concentrations of soy isoflavones (SIF) (50, 100 or 200mg/kg body weight, orally, once per day) from weaning until sexual maturity (3 months) in order to evaluate the influence of SIF on ovarian follicle development. After treatment, the serum sex hormone levels and enumeration of ovarian follicles of the ovary were measured. The metabolic profile of follicular fluid was determined using HPLC-MS. Principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA) was used to identify differences in metabolites and reveal useful toxic biomarkers. The results indicated that modest doses of SIF affect ovarian follicle development, as demonstrated by decreased serum estradiol levels and increases in both ovarian follicle atresia and corpora lutea number in the ovary. SIF treatment-related metabolic alterations in follicular fluid were also found in the PCA and PLS-DA models. The 24 most significantly altered metabolites were identified, including primary sex hormones, amino acids, fatty acids and metabolites involved in energy metabolism. These findings may indicate that soy isoflavones affect ovarian follicle development by inducing metabolomic variations in the follicular fluid.
authors: Department of Nutrition and Health Care, School of Public Health, Fujian Medical University, Fuzhou, Fujian, PR China.
authors: Wenxiang
authors: W
authors: Wang
authors: Wenchang
authors: W
authors: Zhang
authors: Jin
authors: J
authors: Liu
authors: Yan
authors: Y
authors: Sun
authors: Yuchen
authors: Y
authors: Li
authors: Hong
authors: H
authors: Li
authors: Shihua
authors: S
authors: Xiao
authors: Xiaohua
authors: X
authors: Shen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005260:Female; D015571:Follicular Fluid / Q000187:drug effects* / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D013058:Mass Spectrometry; D055432:Metabolomics; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development; D010053:Ovary / Q000187:drug effects / Q000254:growth & development; D048789:Phytoestrogens / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max; D014599:Uterus / Q000187:drug effects / Q000254:growth & development
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens