title: Emergence and characterisation of pandemic H1N1 influenza viruses in Hungarian swine herds.
abstract: In 2010, two novel porcine H1N1 influenza viruses were isolated from pigs with influenza-like illness in Hungarian swine herds. Sequence and phylogenetic analysis of these strains revealed that they shared molecular features with the pandemic H1N1 influenza virus strains, which emerged globally during 2009. The PB2, HA and NA genes contained unique amino acid changes compared to the available new H1N1 influenza virus sequences of pig origin. Furthermore, the investigated strains could be separated with respect to parallel amino acid substitutions affecting the polymerase genes (PB2, PB1 and PA) and the nucleoprotein (NP) gene, supporting the proposed complementarities between these proteins, all required for the viral fitness. Molecular characterisation of two Hungarian human pandemic H1N1 isolates was also performed, so that we could compare contemporaneous strains of different host species origins. Shared molecular motifs in various genes of animal and human influenza strains suggested that the Hungarian porcine strains could have originated from humans through direct interspecies transmission. This study is among the few that support the natural human-to-pig transmission of the pandemic H1N1 influenza virus.
authors: Central Agricultural Office Veterinary Diagnostic Directorate Tábornok u. 2 H-1149 Budapest Hungary.
authors: Adám
authors: A
authors: Bálint
authors: István
authors: I
authors: Kiss
authors: Krisztián
authors: K
authors: Bányai
authors: Imre
authors: I
authors: Biksi
authors: Katalin
authors: K
authors: Szentpáli-Gavallér
authors: Tibor
authors: T
authors: Magyar
authors: István
authors: I
authors: Jankovics
authors: Mónika
authors: M
authors: Rózsa
authors: Bálint
authors: B
authors: Szalai
authors: Mária
authors: M
authors: Takács
authors: Adám György
authors: AG
authors: Tóth
authors: Adám
authors: A
authors: Dán
mesh_terms: D000818:Animals; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D009976:Orthomyxoviridae Infections; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins