title: Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters.
abstract: Metabolic adaptations and changes in the expression of nutrient transporters are known to accompany tumorigenic processes. Nevertheless, in the context of solid tumors, studies of metabolism are hindered by a paucity of tools allowing the identification of cell surface transporters on individual cells. Here, we developed a method for the dissociation of human breast cancer tumor xenografts combined with quantification of cell surface markers, including metabolite transporters. The expression profiles of four relevant nutrient transporters for cancer cells' metabolism, Glut1, ASCT2, PiT1 and PiT2 (participating to glucose, glutamine and inorganic phosphate, respectively), as detected by new retroviral envelope glycoprotein-derived ligands, were distinctive of each tumor, unveiling underlying differences in metabolic pathways. Our tumor dissociation procedure and nutrient transporter profiling technology provides opportunities for future basic research, clinical diagnosis, prognosis and evaluation of therapeutic responses, as well as for drug discovery and development.
authors: Institut de Génétique Moléculaire de Montpellier IGMM, CNRS, Montpellier, France.
authors: Vincent
authors: V
authors: Petit
authors: Gérald
authors: G
authors: Massonnet
authors: Zofia
authors: Z
authors: Maciorowski
authors: Jawida
authors: J
authors: Touhami
authors: Aurélie
authors: A
authors: Thuleau
authors: Fariba
authors: F
authors: Némati
authors: Julie
authors: J
authors: Laval
authors: Sophie
authors: S
authors: Château-Joubert
authors: Jean-Luc
authors: JL
authors: Servely
authors: David
authors: D
authors: Vallerand
authors: Jean-Jacques
authors: JJ
authors: Fontaine
authors: Naomi
authors: N
authors: Taylor
authors: Jean-Luc
authors: JL
authors: Battini
authors: Marc
authors: M
authors: Sitbon
authors: Didier
authors: D
authors: Decaudin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D026901:Membrane Transport Proteins / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D015203:Reproducibility of Results; D014183:Transplantation, Heterologous
chemical_list: D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D026901:Membrane Transport Proteins