title: Bioproduction of prostaglandins in a transgenic liverwort, Marchantia polymorpha.
abstract: Prostaglandins are biologically active substances used in a wide range of medical treatments. Prostaglandins have been supplied mainly by chemical synthesis; nevertheless, the high cost of prostaglandin production remains a factor. To lower the cost of prostaglandin production, we attempted to produce prostaglandins using a liverwort, Marchantia polymorpha L., which accumulates arachidonic acid, which is known as a substrate of prostaglandins. Here we report the first bioproduction of prostaglandins in plant species by introducing a cyclooxygenase gene from a red alga, Gracilaria vermiculophylla into the liverwort. The transgenic liverworts accumulated prostaglandin F2α, prostaglandin E2 and prostaglandin D2 which were not detected in the wild-type liverwort. Moreover, we succeeded in drastically increasing the bioproduction of prostaglandins using an in vitro reaction system with the extracts of transgenic liverworts.
authors: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa, 921-8836, Japan, mtake@ishikawa-pu.ac.jp.
authors: Miho
authors: M
authors: Takemura
authors: Hirosuke
authors: H
authors: Kanamoto
authors: Shingo
authors: S
authors: Nagaya
authors: Kanji
authors: K
authors: Ohyama
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002853:Chromatography, Liquid; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D018014:Gene Transfer Techniques; D044724:Gracilaria / Q000201:enzymology; D047008:Marchantia / Q000737:chemistry / Q000235:genetics*; D015394:Molecular Structure; D030821:Plants, Genetically Modified / Q000737:chemistry*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D011453:Prostaglandins / Q000096:biosynthesis* / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry
chemical_list: D017931:DNA Primers; D011453:Prostaglandins; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases