title: Developmental potential of cloned goat embryos from an SSEA3(+) subpopulation of skin fibroblasts.
abstract: Previous studies have demonstrated that skin stem cells expressing the pluripotency marker stage-specific embryonic antigen 3 (SSEA3) are easier to reprogram into induced pluripotent stem cells (iPSCs) than skin fibroblasts. Furthermore, it is widely speculated that the undifferentiated state may make stem cells more efficient donor cells for somatic cell nuclear transfer (SCNT). In this study, we isolated SSEA3(+) cells from goat skin fibroblast cells (SFCs) using fluorescence-activated cell sorting (FACS) and examined expression of pluripotency markers and in vitro development of cloned embryos following SCNT. Results showed that cell clusters from SSEA3(+) cells were consistently positive for alkaline phosphatase staining and pluripotency markers, Nanog, Oct4, Sox2, and SSEA3. The cleavage rate of cloned embryos derived from SSEA3(+) cells did not differ compared with SFCs (70.5±0.8% and 68.4±2.1%, respectively), but was significantly higher compared with SSEA3(-) cells (64.9±1.6%, p<0.05). The blastocyst rate was significantly increased in the SSEA3(+) cell group compared with the SFC and SSEA3(-) cell groups (30.3±1.2% vs. 21.2±0.9 and 19.0±1.0%, respectively, p<0.05). The quality of cloned blastocysts from SSEA3(+) cells was higher compared with SFCs and SSEA3(-) cells, based on total cell number and number of apoptotic cells per blastocyst. These findings suggest that using SSEA3(+) cells as donors for SCNT is beneficial for enhancing in vitro development and quality of cloned goat embryos.
authors: College of Veterinary Medicine, Northwest A&F University, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi Province, PR China.
authors: Jun
authors: J
authors: Liu
authors: Zhongcai
authors: Z
authors: Yang
authors: Mingning
authors: M
authors: Qiu
authors: Yan
authors: Y
authors: Luo
authors: Meijun
authors: M
authors: Pang
authors: Yongyan
authors: Y
authors: Wu
authors: Yong
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002455:Cell Division; D019976:Cloning, Organism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006041:Goats; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D055746:Stage-Specific Embryonic Antigens; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D055746:Stage-Specific Embryonic Antigens; D014157:Transcription Factors; C052924:stage-specific embryonic antigen-3