title: Pinacidil stimulates osteoblast function in osteoblastic MC3T3-E1 cells.
abstract: This study examined the effect of pinacidil, a nonselective adenosine triphosphate-sensitive potassium channel opener, on the function of osteoblastic MC3T3-E1 cells. Pinacidil caused a significant elevation of collagen synthesis, alkaline phosphatase activity, osteocalcin synthesis and mineralization in the cells (p < 0.05). Pinacidil significantly decreased the production of osteoclast differentiation inducing factors such as TNF-α, IL-6 and receptor activator of nuclear factor-κB ligand in the presence of antimycin A, which inhibits mitochondrial electron transport. Moreover, pinacidil prevented antimycin A-induced reactive oxygen species and nitrotyrosine production. These results demonstrate that pinacidil may have positive effects on skeletal structure.
authors: Research Institute of Endocrinology, Kyung Hee University Hospital, Seoul, Republic of Korea.
authors: Kwang Sik
authors: KS
authors: Suh
authors: Young Soon
authors: YS
authors: Lee
authors: Eun Mi
authors: EM
authors: Choi
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis; D016207:Cytokines / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D015640:Ion Channel Gating / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology*; D015675:Osteocalcin / Q000096:biosynthesis; D020110:Pinacidil / Q000494:pharmacology*; D015221:Potassium Channels / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D016207:Cytokines; D015221:Potassium Channels; D053245:RANK Ligand; D017382:Reactive Oxygen Species; C505689:Tnfsf11 protein, mouse; D015675:Osteocalcin; C002744:3-nitrotyrosine; D014443:Tyrosine; D000968:Antimycin A; D020110:Pinacidil; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium