title: Quality assessment of SPR sensor chips; case study on L1 chips.
abstract: Surface quality of the Surface Plasmon Resonance (SPR) chips is a major limiting issue in most SPR analyses, even more for supported lipid membranes experiments, where both the organization of the lipid matrix and the subsequent incorporation of the target molecule depend on the surface quality. A novel quantitative method to characterize the quality of SPR sensors chips is described for L1 chips subject to formation of lipid films, injection of membrane disrupting compounds, followed by appropriate regeneration procedures. The method consists in analysis of the SPR reflectivity curves for several standard solutions (e.g. PBS, HEPES or deionized water). This analysis reveals the decline of sensor surface as a function of the number of experimental cycles (consisting in biosensing assay and regeneration step) and enables active control of surface regeneration for enhanced reproducibility. We demonstrate that quantitative evaluation of the changes in reflectivity curves (shape of the SPR dip) and of the slope of the calibration curve provides a rapid and effective procedure for surface quality assessment. Whereas the method was tested on L1 SPR sensors chips, we stress on its amenability to assess the quality of other types of SPR chips, as well.
authors: International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101, Bucharest 6, Romania.
authors: Andreea
authors: A
authors: Olaru
authors: Mihaela
authors: M
authors: Gheorghiu
authors: Sorin
authors: S
authors: David
authors: Cristina
authors: C
authors: Polonschii
authors: Eugen
authors: E
authors: Gheorghiu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D008563:Membrane Lipids / Q000737:chemistry / Q000302:isolation & purification*; D008566:Membranes / Q000737:chemistry; D046208:Microchip Analytical Procedures; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D008563:Membrane Lipids