title: Exploring novel ultrafine Eri silk bioscaffold for enzyme stabilisation in cellobiose hydrolysis.
abstract: The suitability of optimised ultrafine Eri silk microparticles as novel enzyme supports was studied for potential application in biofuel production. β-glucosidase (BGL) from Aspergillus niger was immobilised on Eri silk fibrion particles via an adsorption method resulting in a 62% immobilisation yield. Soluble and immobilised enzymes exhibited pH-optima at pH 4.0 and 5.0, respectively with optimum activity at 60°C. The Michaelis constant (K(M)) was 0.16 and 0.27 mM for soluble and immobilised BGL respectively. The immobilisation support has a protective effect on the enzyme by increasing rigidity; this is reflected by an increase in stability under thermal denaturation at 70°C. Immobilised enzyme retained more than 50% of initial activity for up to eight cycles. Maximum cellobiose hydrolysis by immobilised BGL was achieved at 20 h. Crystalline ultrafine Eri silk particles were found to be a promising viable, environmentally sound and stable matrix for binding BGL for cellobiose hydrolysis.
authors: Centre for Chemistry and Biotechnology (CCB), Geelong Technology Precinct, Deakin University, Geelong, Victoria 3217, Australia.
authors: Madan L
authors: ML
authors: Verma
authors: Rangam
authors: R
authors: Rajkhowa
authors: Xungai
authors: X
authors: Wang
authors: Colin J
authors: CJ
authors: Barrow
authors: Munish
authors: M
authors: Puri
mesh_terms: D000327:Adsorption; D001234:Aspergillus niger / Q000201:enzymology*; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002475:Cellobiose / Q000378:metabolism; D004795:Enzyme Stability / Q000502:physiology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D047011:Silk / Q000737:chemistry*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D047011:Silk; D002475:Cellobiose; D001617:beta-Glucosidase
keywords: Biofuel; Immobilisation; Lignocellulose; Nanobiotechnology; β-Gucosidase