title: Development of a subcritical fluid extraction and GC-MS validation method for polychlorinated biphenyls (PCBs) in marine samples.
abstract: This paper describes a new procedure for extracting polychlorinated biphenyls (PCBs) from marine samples using subcritical 1,1,1,2-tetrafluoroethane (R134a). The extraction procedure was optimized at temperatures varying from 20 to 70°C and pressures ranging from 3 to 15 MPa. The volume of the co-solvent was then optimized using 1,1,1,2-tetrafluoroethane (R134a) as the subcritical phase. PCBs were characterized by GC-MS using the optimized conditions of 3 MPa, 30°C, and a co-solvent volume of 6 mL. The average yields of PCBs from subcritical fluid extraction of spiked oyster samples were measured and found to be greater than 90%, with relative standard deviations (RSD) of less than 10%. Detection limits of this method were in the range of 0.045-0.108 ng/g of dry mass. The method was compared to Soxhlet extraction and then applied for monitoring PCBs in oysters from Qingdao, Shandong, China.
authors: Department of Food Science and Engineering, Ocean University of China, Qingdao, China.
authors: Kai
authors: K
authors: Jia
authors: Xiaomei
authors: X
authors: Feng
authors: Kun
authors: K
authors: Liu
authors: Yuqian
authors: Y
authors: Han
authors: Yong
authors: Y
authors: Xue
authors: Changhu
authors: C
authors: Xue
mesh_terms: D000818:Animals; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D010125:Ostreidae / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000302:isolation & purification*; D012044:Regression Analysis; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis; D014874:Water Pollutants, Chemical
chemical_list: D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls