title: Identification of Alternaria alternata mycotoxins by LC-SPE-NMR and their cytotoxic effects to soybean (Glycine max) cell suspension culture.
abstract: This present work describes the application of liquid chromatography-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC(50) values which range from 0.11 (± 0.02) to 4.69 (± 0.47) μM showed the high cytotoxicity of these compounds.
authors: Laboratório de Bioquímica Micromolecular de Micro-organismos, Departamento de Química, Universidade Federal de São Carlos-UFSCar, Rodovia Washington Luiz, Km 235, 676 São Carlos-SP, Brazil. gezimar@accert.com
authors: Gezimar D
authors: GD
authors: de Souza
authors: Axel
authors: A
authors: Mithöfer
authors: Cristina
authors: C
authors: Daolio
authors: Bernd
authors: B
authors: Schneider
authors: Edson
authors: E
authors: Rodrigues-Filho
mesh_terms: D000528:Alternaria / Q000737:chemistry*; D018929:Cell Culture Techniques; D002457:Cell Extracts / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D009183:Mycotoxins / Q000737:chemistry* / Q000633:toxicity; D019906:Nuclear Magnetic Resonance, Biomolecular; D052616:Solid Phase Extraction; D013025:Glycine max / Q000187:drug effects*
chemical_list: D002457:Cell Extracts; D009183:Mycotoxins