title: Immunoanalysis methods for the detection of dioxins and related chemicals.
abstract: With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. tiantianstacy@163.com
authors: Wenjing
authors: W
authors: Tian
authors: Heidi Qunhui
authors: HQ
authors: Xie
authors: Hualing
authors: H
authors: Fu
authors: Xinhui
authors: X
authors: Pei
authors: Bin
authors: B
authors: Zhao
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D004147:Dioxins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D018336:Receptors, Aryl Hydrocarbon / Q000737:chemistry / Q000276:immunology
chemical_list: D000906:Antibodies; D004147:Dioxins; D018336:Receptors, Aryl Hydrocarbon