title: Shigella in Brazilian children with acute diarrhoea: prevalence, antimicrobial resistance and virulence genes.
abstract: Diarrhoeal disease is still considered a major cause of morbidity and mortality among children. Among diarrhoeagenic agents, Shigella should be highlighted due to its prevalence and the severity of the associated disease. Here, we assessed Shigella prevalence, drug susceptibility and virulence factors. Faeces from 157 children with diarrhoea who sought treatment at the Children's Hospital João Paulo II, a reference children´s hospital in Belo Horizonte, state of Minas Gerais, Brazil, were cultured and drug susceptibility of the Shigella isolates was determined by the disk diffusion technique. Shigella virulence markers were identified by polymerase chain reaction. The bacterium was recovered from 10.8% of the children (88.2% Shigella sonnei). The ipaH, iuc, sen and ial genes were detected in strains isolated from all shigellosis patients; set1A was only detected in Shigella flexneri. Additionally, patients were infected by Shigella strains of different ial, sat, sen and set1A genotypes. Compared to previous studies, we observed a marked shift in the distribution of species from S. flexneri to S. sonnei and high rates of trimethoprim/sulfamethoxazole resistance.
authors: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors: Mireille Ângela Bernardes
authors: MÂ
authors: Sousa
authors: Edilberto Nogueira
authors: EN
authors: Mendes
authors: Guilherme Birchal
authors: GB
authors: Collares
authors: Luciano Amedée
authors: LA
authors: Péret-Filho
authors: Francisco José
authors: FJ
authors: Penna
authors: Paula Prazeres
authors: PP
authors: Magalhães
mesh_terms: D000208:Acute Disease; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology* / Q000517:prevention & control; D052978:Disk Diffusion Antimicrobial Tests; D004405:Dysentery, Bacillary / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012760:Shigella / Q000145:classification / Q000187:drug effects / Q000472:pathogenicity*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors; D000667:Ampicillin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination