title: A straightforward immunoassay applicable to a wide range of antibodies based on surface enhanced fluorescence.
abstract: A straightforward immunoassay based on surface enhanced fluorescence (SEF) has been demonstrated using a fluorescent immune substrate and antibody functionalized-silver nanoparticles. Unlike the conventional SEF-based immunoassay, which usually uses the dye-labeled antibodies and the metallic nanostructured-substrates, the presented immune system does not need the antibodies to be labeled with dye molecules. Thus, this immunoassay can be easily applied to the detection of a wide range of target antigens, which is of great importance for its practical application. The experimental results show that this immunoassay has a good specificity as well as the capacity of quantitative detection. Basically, the surface density of the immuno-adsorbed silver nanoparticles increases with the increased amount of target antigens, resulting in a fluorescence enhancement up to around 7 fold. The dose-responsive performance of the immunoassay has been investigated and the limit of detection (LOD) is 1 ng/mL. Due to its simple preparation method and the wide range of detectable antigens, this presented immunoassay is expected to be helpful for extending the SEF-based application.
authors: Advanced Photonics Center, Southeast University, Nanjing, People's Republic of China.
authors: Ruohu
authors: R
authors: Zhang
authors: Zhuyuan
authors: Z
authors: Wang
authors: Chunyuan
authors: C
authors: Song
authors: Jing
authors: J
authors: Yang
authors: Yiping
authors: Y
authors: Cui
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013499:Surface Properties
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes