title: Production of a sterilised decellularised tendon allograft for clinical use.
abstract: Application of a high-level decontamination or sterilisation procedure and cell removal technique to tendon allograft can reduce the concerns of disease transmission, immune reaction, and may improve remodelling of the graft after implantation. The decellularised matrix can also be used as a matrix for tendon tissue engineering. One such sterilisation factor, Peracetic acid (PAA) has the advantage of not producing harmful reaction residues. The aim of this study was to evaluate the effects of PAA treatment and a cell removal procedure on the production of tendon matrix. Human patellar tendons, thawed from frozen were treated respectively as: Group 1, control with no treatment; Group 2, sterilised with PAA (0.1 % (w/v) PAA for 3 h) Group 3, decellularised (incubation successively in hypotonic buffer, 0.1 % (w/v) sodium dodecyl sulphate, and a nuclease solution); Group 4, decellularised and PAA sterilised. Histological analysis showed that no cells were visible after the decellularisation treatment. The integrity of tendon structure was maintained after decellularisation and PAA sterilisation, however, the collagen waveform was slightly loosened. No contact cytotoxicity was found in any of the groups. Determination of de-natured collagen showed no significant increase when compared with the control. This suggested that the decellularisation and sterilisation processing procedures did not compromise the major properties of the tendon. The sterilised, decellularised tendon could be suitable for clinical use.
authors: Tissue Development Laboratory, NHS Blood and Transplant, Estuary Banks, Speke, Liverpool, L24 8RB, UK.
authors: Q
authors: Q
authors: Huang
authors: E
authors: E
authors: Ingham
authors: P
authors: P
authors: Rooney
authors: J N
authors: JN
authors: Kearney
mesh_terms: D064591:Allografts / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D001672:Biocompatible Materials / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D006801:Humans; D011489:Protein Denaturation / Q000187:drug effects; D012995:Solubility; D013242:Sterilization / Q000379:methods*; D013710:Tendons / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D003094:Collagen