title: Semen analysis of Golden Retriever healthy dogs and those affected by muscular dystrophy.
abstract: This study aimed to characterise canine flow cytometry semen analysis, as well as seminal reactive oxygen species dosage using the Golden Retriever breed as model of study. Moreover, we searched for the influence of muscular dystrophy in Golden Retriever dogs on semen parameters. Thirty-seven semen samples were obtained from healthy Golden Retrievers (n = 15) and from muscular dystrophy affected dogs (n = 22). Sperm-rich fractions were analysed by standardised breeding soundness examination in addition to the assay of fluorescence assisted cell sorting for acrosome integrity, mitochondrial activity and DNA fragmentation. Volume of ejaculate, per cent of motile spermatozoa and vigour were similar between groups; there were no differences in the per cent of minor and major defects. Integrity of acrosomal membrane, mitochondrial potential and sperm DNA fragmentation had no significant differences between groups either. Animals from control group had higher concentration of spontaneous seminal oxidative species in comparison with affected animals. Dogs affected by dystrophy had seminal parameters similar to those observed in healthy dogs except for the lower concentration of oxidative species. Future studies aiming to establish reference values for canine seminal parameters should be considered preferably with distinction of breeds.
authors: Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors: M A
authors: MA
authors: Peres
authors: A M
authors: AM
authors: da Rocha
authors: C I
authors: CI
authors: Vannucchi
authors: C M
authors: CM
authors: Mendes
authors: P V
authors: PV
authors: Cavalcanti
authors: M
authors: M
authors: Nichi
authors: C E
authors: CE
authors: Ambrosio
authors: M A
authors: MA
authors: Miglino
authors: J A
authors: JA
authors: Visintin
authors: M E O
authors: ME
authors: D'Ávila Assumpção
mesh_terms: D000177:Acrosome / Q000378:metabolism; D000818:Animals; D016022:Case-Control Studies; D053938:DNA Fragmentation; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005434:Flow Cytometry; D008297:Male; D053078:Membrane Potential, Mitochondrial; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012661:Semen / Q000378:metabolism; D055101:Semen Analysis / Q000592:standards / Q000662:veterinary*; D013094:Spermatozoa / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species
keywords: Dog semen; GRMD; ROS; TBARS; flow cytometry