title: Performance of the GenoType MTBDRplus assay directly on sputum specimens from Brazilian patients with tuberculosis treatment failure or relapse.
abstract: Rapid identification of drug resistance in clinical isolates of Mycobacterium tuberculosis is important in determining treatment for tuberculosis. The aim of this work was evaluate the performance of the GenoType MDRTBplus assay directly on sputum of patients who had treatment failure or relapse in a routine outpatient setting in southern Brazil.
authors: Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brazil. raquel368@gmail.com
authors: Raquel de Abreu
authors: Rde A
authors: Maschmann
authors: Fernanda
authors: F
authors: Sá Spies
authors: Luciana de Souza
authors: Lde S
authors: Nunes
authors: Andrezza Wolowski
authors: AW
authors: Ribeiro
authors: Taís Raquel Marcon
authors: TR
authors: Machado
authors: Arnaldo
authors: A
authors: Zaha
authors: Maria Lucia Rosa
authors: ML
authors: Rossetti
mesh_terms: D000904:Antibiotics, Antitubercular / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001938:Brazil; D002374:Catalase / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D007538:Isoniazid / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D025202:Molecular Diagnostic Techniques; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D010088:Oxidoreductases / Q000235:genetics; D012008:Recurrence; D012293:Rifampin / Q000627:therapeutic use; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology*; D017211:Treatment Failure; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000904:Antibiotics, Antitubercular; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C505805:rpoB protein, Mycobacterium tuberculosis; D010088:Oxidoreductases; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; C085257:InhA protein, Mycobacterium; D012321:DNA-Directed RNA Polymerases; D007538:Isoniazid; D012293:Rifampin