title: Structural features and bioremediation activity of an exopolysaccharide produced by a strain of Enterobacter ludwigii isolated in the Chernobyl exclusion zone.
abstract: The bacterium Enterobacter ludwigii Ez-185-17, member of the family Enterobacteriaceae, was isolated from the root nodules of plants harvested in the nuclear power region of Chernobyl. Under batch culture conditions, the bacteria produce a high-molecular-mass exopolysaccharide (EPS). After purification, the structure of this EPS was determined using a combinatory approach including monosaccharide composition (GC-FID, HPAEC-PAD) and branching structure determination (GC-MS), as well as 1D/2D NMR ((1)H, (13)C) and ESI-MS (HR, MS/MS) studies of oligosaccharides obtained from mild acid hydrolysis. The EPS was found to be a charged hexasaccharide with a repeating unit composed of d-galactose, d-glucose, l-fucose, d-glucuronic acid (2:1:2:1) and substituted with acyl and pyruvyl groups. The metal-binding properties of the exopolysaccharide were then investigated, and the results seem to indicate that the EPS decreased Cd sequestration in flax seeds.
authors: BIOPI, Université de Picardie Jules Verne, 33, Rue St Leu, F-80039 Amiens, France.
authors: Corinne
authors: C
authors: Pau-Roblot
authors: Michelle
authors: M
authors: Lequart-Pillon
authors: Ludovic
authors: L
authors: Apanga
authors: Serge
authors: S
authors: Pilard
authors: Josiane
authors: J
authors: Courtois
authors: Nathalie
authors: N
authors: Pawlicki-Jullian
mesh_terms: D001673:Biodegradation, Environmental; D002104:Cadmium / Q000737:chemistry; D047852:Chernobyl Nuclear Accident; D004754:Enterobacter / Q000737:chemistry* / Q000302:isolation & purification* / Q000502:physiology; D019597:Flax / Q000737:chemistry; D005643:Fucose / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D011135:Polysaccharides, Bacterial; D002104:Cadmium; D005643:Fucose; D020723:Glucuronic Acid; D005947:Glucose; D005690:Galactose