title: Iris Nertschinskia ethanol extract differentially induces cytotoxicity in human breast cancer cells depending on AKT1/2 activity.
abstract: Recently, we reported that an ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line. However, the detailed mechanisms were not fully explored. Here, we demonstrate another aspect of the activity of I. nertschinskia in breast cancer cells. We compared the response to an ethanol extract of I. nertschinskia in two different human breast cancer cell lines, Hs578Tand MDA-MB231, respectively with relatively low and high AKT1/2 activity by trypan blue exclusion assay and FACS analysis. Knockdown of endogenous AKT1 or AKT2 in breast cancer cells by RNA interference determined the sensitivity to I. nertschinskia ethanol extract compared to control cells. The I. nertschinskia ethanol extract induced cell death in a manner that depended on the level of phosphorylated AKT1/2 protein and was associated with a significant increase in the sub-G1 cell population, indicative of apoptosis. Our results indicate that an ethanol extract of I. nertschinskia differentially induces cell death in breast cancer cells depending on their level of phosphorylated AKT1/2.
authors: Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center, Seoul, Republic of Korea.
authors: Jae-Sik
authors: JS
authors: Shin
authors: Hyung-Gun
authors: HG
authors: Maeng
authors: Seung-Woo
authors: SW
authors: Hong
authors: Jai-Hee
authors: JH
authors: Moon
authors: Jin-Sun
authors: JS
authors: Kim
authors: Young-Ah
authors: YA
authors: Suh
authors: Eun-Sung
authors: ES
authors: Kim
authors: Young-Min
authors: YM
authors: Lee
authors: Ye-Seul
authors: YS
authors: Kim
authors: Eun-Kyung
authors: EK
authors: Choi
authors: Inki
authors: I
authors: Kim
authors: Sok-Young
authors: SY
authors: Lee
authors: Dong-Hyung
authors: DH
authors: Cho
authors: Nam-Joo
authors: NJ
authors: Hong
authors: Tae-Won
authors: TW
authors: Kim
authors: Dong-Hoon
authors: DH
authors: Jin
authors: Wang Jae
authors: WJ
authors: Lee
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D000431:Ethanol / Q000737:chemistry*; D005260:Female; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D031323:Iris Plant / Q000737:chemistry*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D000431:Ethanol; C494918:AKT1 protein, human; C494921:AKT2 protein, human; D051057:Proto-Oncogene Proteins c-akt