title: Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae).
abstract: A new member of short chain α-neurotoxic protein family from venom of the Mexican coral snake, Micrurus laticollaris, was characterized. This protein, named MlatA1, possesses 61 amino acids with 8 conserved cysteine residues, sharing 30-91% sequence identity with other fully sequenced Micrurus toxins. MlatA1 (LD50i.v. = 0.064 mg/kg) antagonizes with both fetal and adult nicotinic acetylcholine receptor (nAChR) as well as α-7 neuronal nAChR in a dose-dependent way. Specific rabbit anti-Mlat serum (titer higher than 18,000) does not show any protective ability against this toxin, nevertheless it was able to recognize protein bands in six out of twelve Micrurus venoms showing the existence of two distinct antigenic groups for α-neurotoxins in North American coral snakes species. The MlatA1 gene was cloned and used to produce recombinant toxin (rMlatA1) that was recognized by rabbit anti-native toxin but was depleted of toxic activity.
authors: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, CP. 62210 Cuernavaca, Morelos, Mexico.
authors: Alejandro
authors: A
authors: Carbajal-Saucedo
authors: Estuardo
authors: E
authors: López-Vera
authors: Melisa
authors: M
authors: Bénard-Valle
authors: Eric N
authors: EN
authors: Smith
authors: Fernando
authors: F
authors: Zamudio
authors: Adolfo R
authors: AR
authors: de Roodt
authors: Alejandro
authors: A
authors: Olvera-Rodríguez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004546:Elapid Venoms / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity; D017815:Elapidae / Q000502:physiology*; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D011817:Rabbits; D011978:Receptors, Nicotinic / Q000187:drug effects* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D021241:Spectrometry, Mass, Electrospray Ionization; D014982:Xenopus laevis
chemical_list: D004546:Elapid Venoms; D011978:Receptors, Nicotinic; D011993:Recombinant Fusion Proteins; C099850:alpha-neurotoxin, Micrurus