title: Cyanobactericidal effect of Streptomyces sp. HJC-D1 on Microcystis auruginosa.
abstract: An isolated strain Streptomyces sp. HJC-D1 was applied to inhibit the growth of cyanobacterium Microcystis aeruginosa FACHB-905. The effect of Streptomyces sp. HJC-D1 culture broth on the cell integrity and physiological characteristics of M. aeruginosa FACHB-905 was investigated using the ﬂow cytometry (FCM), enzyme activity and transmission electron microscopy (TEM) methods. Results showed that the growth of M. aeruginosa FACHB-905 was significantly inhibited, and the percentage of live cells depended on the culture broth concentration and exposure time. The activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) increased with exposure concentration and exposure time, and the significant increase of reactive oxygen species (ROS) led to the disruption of the subcellular structure of M. aeruginosa FACHB-905, and caused the increase of malondialdehyde (MDA). Furthermore, TEM observation suggested the presence of three stages (cell breakage, organelle release and cell death) for the cyanobactericidal process of Streptomyces sp. HJC-D1. Therefore, Streptomyces sp. HJC-D1 not only affected antioxidant enzyme activities and ROS level, but also destroyed the subcellular structure of M. aeruginosa FACHB-905, demonstrating excellent cyanobactericidal properties.
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, China.
authors: Yun
authors: Y
authors: Kong
authors: Xiangyang
authors: X
authors: Xu
authors: Liang
authors: L
authors: Zhu
mesh_terms: D050260:Carbohydrate Metabolism; D002374:Catalase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D050296:Microbial Viability; D046931:Microcystis / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013302:Streptomyces / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase