title: Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells cultured with umbilical cord blood-derived fibrin: a preliminary study.
abstract: This study examined the potential for osteogenesis via regenerative medicine using autologous tissues (umbilical cord (UC) and umbilical cord blood (UCB)) in nude mice. The study was designed to provide the three elements required for regenerative medicine (cell, scaffold, and growth factor) and autoserum for culture by means of autologous tissues. Mesenchymal stromal cells were obtained from UC (UC-MSCs). Fibrin, platelet-rich-plasma, and autoserum were obtained from UCB as scaffold, growth factor and serum for culture respectively. UC-MSCs were obtained from Wharton jelly and cultured with UCB-derived fibrin (UCB-fibrin) for 3-4 weeks to induce their differentiation into osteoblasts. They were implanted subcutaneously into the dorsum of male nude mice for 6 weeks prior to undergoing assessment. The assessments performed were haematoxylin and eosin, and alizarin red staining, immunohistochemical staining of human mitochondria, scanning electron microscopy, scanning electron microscopy with energy dispersive X-ray spectrometry and real-time reverse transcriptase-polymerase chain reaction to assess the expressions of osteoblast markers. Consequently, the differentiation of UC-MSCs into osteoblasts and the production of hydroxyapatite were verified. This study suggested the possible formation of bone tissue using biomedical materials obtained from UC and UCB.
authors: Department of Plastic and Aesthetic Surgery, School of Medicine, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: kyokobaba@kitasato-u.ac.jp.
authors: Kyoko
authors: K
authors: Baba
authors: Yasuharu
authors: Y
authors: Yamazaki
authors: Masashi
authors: M
authors: Ishiguro
authors: Kenichi
authors: K
authors: Kumazawa
authors: Kazuya
authors: K
authors: Aoyagi
authors: Shigehiro
authors: S
authors: Ikemoto
authors: Akira
authors: A
authors: Takeda
authors: Eiju
authors: E
authors: Uchinuma
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D003470:Culture Media; D005312:Fetal Blood / Q000166:cytology*; D005337:Fibrin / Q000502:physiology*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology*; D053657:Platelet-Rich Plasma / Q000502:physiology; D013052:Spectrometry, X-Ray Emission; D040521:Subcutaneous Tissue / Q000601:surgery; D054457:Tissue Scaffolds; D014470:Umbilical Cord / Q000166:cytology*; D059631:Wharton Jelly / Q000166:cytology; D055114:X-Ray Microtomography
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D003470:Culture Media; D015675:Osteocalcin; D005337:Fibrin; D000469:Alkaline Phosphatase
keywords: Autologous tissue; Fibrin; Mesenchymal stromal cell; Osteogenesis; Regenerative medicine; Umbilical cord blood