title: Construction of an infectious cDNA clone of radish mosaic virus, a crucifer-infecting comovirus.
abstract: Radish mosaic virus (RaMV) is a crucifer-infecting comovirus that has been detected worldwide. Here, we report the successful construction of a full-length infectious cDNA clone of RaMV. The full-length cDNA clones corresponding to RNA1 and RNA2 of a Japanese isolate of RaMV were cloned into the pBlueScript plasmid or the binary vector pCAMBIA1301 downstream of the cauliflower mosaic virus 35S promoter. Mechanical inoculation or agroinoculation of Nicotiana benthamiana with these vectors resulted in systemic RaMV infections causing symptoms similar to those caused by the wild-type parental virus. The presence of progeny virus was verified by western blot analysis and electron microscopy.
authors: Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
authors: Ken
authors: K
authors: Komatsu
authors: Masayoshi
authors: M
authors: Hashimoto
authors: Yukari
authors: Y
authors: Okano
authors: Takuya
authors: T
authors: Keima
authors: Yugo
authors: Y
authors: Kitazawa
authors: Takamichi
authors: T
authors: Nijo
authors: Shuichiro
authors: S
authors: Takahashi
authors: Kensaku
authors: K
authors: Maejima
authors: Yasuyuki
authors: Y
authors: Yamaji
authors: Shigetou
authors: S
authors: Namba
mesh_terms: D015153:Blotting, Western; D017798:Comovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D018076:DNA, Complementary / Q000235:genetics*; D008854:Microscopy, Electron; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D031224:Raphanus / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral