title: Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator.
abstract: Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds up to video-rate, but it sacrifices chemical specificity in samples with overlapping bands compared to broadband (multiplex) excitation. We develop a rapidly tunable picosecond optical parametric oscillator with an electro-optical tunable Lyot filter, and demonstrate multicolor SRS microscopy with synchronized line-by-line wavelength tuning to avoid spectral artifacts due to sample movement. We show sensitive imaging of three different kinds of polymer beads and live HeLa cells with moving intracellular lipid droplets.
authors: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors: Lingjie
authors: L
authors: Kong
authors: Minbiao
authors: M
authors: Ji
authors: Gary R
authors: GR
authors: Holtom
authors: Dan
authors: D
authors: Fu
authors: Christian W
authors: CW
authors: Freudiger
authors: X Sunney
authors: XS
authors: Xie
mesh_terms: D016477:Artifacts; D003952:Diagnostic Imaging / Q000379:methods; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008853:Microscopy / Q000379:methods*; D009991:Oscillometry / Q000379:methods*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014227:Triazines / Q000737:chemistry
chemical_list: D008055:Lipids; D011137:Polystyrenes; D014227:Triazines; D019904:Polymethyl Methacrylate; C011907:melamine