title: Microwave-mediated reduction of disulfide bridges with supported (tris(2-carboxyethyl)phosphine) as resin-bound reducing agent.
abstract: We report on the synthesis and use of a new supported reagent consisting in tris(2-carboxyethyl)phosphine (TCEP) immobilized on hydrophilic PEG based resin beads. Used in conjunction with a 5 min microwave (MW) irradiation, "supported TCEP" reduced disulfide bridges in free thiols in peptides having two or more cysteine residues. Separation of reaction products from reducing agent was easily performed by simple filtration.
authors: Institut des Biomolécules Max Mousseron IBMM, UMR 5247, Université Montpellier 1, Université Montpellier 2, CNRS, 15 avenue Charles Flahault 34000 Montpellier, France.
authors: Guillaume
authors: G
authors: Miralles
authors: Pascal
authors: P
authors: Verdié
authors: Karine
authors: K
authors: Puget
authors: Amélie
authors: A
authors: Maurras
authors: Jean
authors: J
authors: Martinez
authors: Gilles
authors: G
authors: Subra
mesh_terms: D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry*; D008872:Microwaves; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D010720:Phosphines / Q000138:chemical synthesis / Q000737:chemistry*; D019163:Reducing Agents / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004220:Disulfides; D010455:Peptides; D010720:Phosphines; D019163:Reducing Agents; C080938:tris(2-carboxyethyl)phosphine