title: Total flavan glycoside from Abacopteris penangiana rhizomes and its acid hydrolysate: characterisation and anti-benign prostatic hyperplasia potential.
abstract: This study was conducted to characterise the flavonoid components of total flavan glycoside from Abacopteris penangiana rhizomes (TFA) and its acid hydrolysate (AHT) through HPLC-DAD-ESI-MS/MS analysis, and to investigate the hypothesis that TFA and AHT exhibit anti-benign prostatic hyperplasia (BPH) potential in castrated rats with testosterone-induced BPH. HPLC-MS/MS analysis indicated that TFA is rich in flavan-4-ol glycosides and AHT mainly contains 3-deoxygenated anthocyanidin. After 4 weeks of administration, TFA and AHT successfully decreased the prostate index and prostate specific antigen plasma concentrations in the rats. Histoarchitectural improvement in the prostate gland was also observed. Reduced dihydrotestosterone, VEGF, bFGF, EGF, and KGF levels were observed both in TFA- and AHT-treated rats. Furthermore, the prostatic expression of Blc-2 was inhibited, whereas that of Bax and p53 was activated by TFA and AHT. In conclusion, TFA and AHT have anti-BPH properties. Hence, plants with flavan glycosides have potential use in the treatment of BPH.
authors: Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Han
authors: H
authors: Wei
authors: Guanghua
authors: G
authors: Wu
authors: Du
authors: D
authors: Shi
authors: Shanshan
authors: S
authors: Song
authors: Xuenong
authors: X
authors: Zhang
authors: Yongfang
authors: Y
authors: Lei
authors: Jinlan
authors: J
authors: Ruan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism; D029624:Ferns / Q000737:chemistry*; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry; D006027:Glycosides / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011470:Prostatic Hyperplasia / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; D004815:Epidermal Growth Factor