title: Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue.
abstract: Three alkyne-containing pyrrolysine derivatives were synthesized and genetically encoded into proteins by a mutant PylRS-tRNA pair with high efficiencies. With these alkyne handles, site-specific dual labeling of proteins can be achieved via a bioorthogonal thiol-yne ligation reaction.
authors: School of Medical Engineering, Hefei University of Technology, Hefei 230009, P. R. China. ymli@hfut.edu.cn
authors: Yiming
authors: Y
authors: Li
authors: Man
authors: M
authors: Pan
authors: Yitong
authors: Y
authors: Li
authors: Yichao
authors: Y
authors: Huang
authors: Qingxiang
authors: Q
authors: Guo
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics*; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D013194:Staining and Labeling / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000480:Alkynes; D029968:Escherichia coli Proteins; D013438:Sulfhydryl Compounds; C084501:hdeA protein, E coli; D000604:Amino Acyl-tRNA Synthetases; C456839:pyrrolysine; D008239:Lysine