title: Regulation of virus-induced inflammatory response by β-carotene in RAW264.7 cells.
abstract: Carotenoids are effective antioxidants, which can quench singlet oxygen, suppress lipid peroxidation, and prevent oxidative damage. Both Pseudorabies virus (PRV) and human Herpes simplex virus (HSV) are DNA viruses, and their pathogenesis and immunobiology are similar. However, PRV does not infect humans. Therefore, PRV was used to infect murine macrophages (RAW264.7 cells), to mimic HSV-induced inflammation. Meanwhile, the influence of β-carotene on PRV-induced inflammation was also investigated. Results indicated that β-carotene inhibited (p<0.05) NO, IL-1β, IL-6, and MCP-1 production in PRV-infected RAW264.7 cells. β-Carotene also suppressed (p<0.05) NF-κB (p50 and p65), phosphorylation of extracellular-signal-related kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) expression. It could be concluded that the anti-inflammatory effect of β-carotene is mainly through a suppression of cytokine expression in PRV-induced inflammation, which results from NF-κB inactivation. β-Carotene can be considered a potential anti-inflammatory agent for DNA-virus infection.
authors: Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
authors: Hui-Wen
authors: HW
authors: Lin
authors: Tien-Jye
authors: TJ
authors: Chang
authors: Deng-Jye
authors: DJ
authors: Yang
authors: Yi-Chen
authors: YC
authors: Chen
authors: Meilin
authors: M
authors: Wang
authors: Yuan-Yen
authors: YY
authors: Chang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D002460:Cell Line; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000276:immunology; D053583:Interleukin-1beta / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D048031:JNK Mitogen-Activated Protein Kinases / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000276:immunology; D011557:Pseudorabies / Q000188:drug therapy / Q000276:immunology* / Q000821:virology; D019207:beta Carotene / Q000008:administration & dosage*; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D019207:beta Carotene; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases