title: A highly efficient microfluidic nano biochip based on nanostructured nickel oxide.
abstract: We present results of the studies relating to fabrication of a microfluidic biosensor chip based on nickel oxide nanorods (NRs-NiO) that is capable of directly measuring the concentration of total cholesterol in human blood through electrochemical detection. Using this chip we demonstrate, with high reliability and in a time efficient manner, the detection of cholesterol present in buffer solutions at clinically relevant concentrations. The microfluidic channel has been fabricated onto a nickel oxide nanorod-based electrode co-immobilized with cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) that serves as the working electrode. Bare indium tin oxide served as the counter electrode. A Ag/AgCl wire introduced to the outlet of the microchannel acts as a reference electrode. The fabricated NiO nanorod-based electrode has been characterized using X-ray diffraction, Raman spectroscopy, HR-TEM, FT-IR, UV-visible spectroscopy and electrochemical techniques. The presented NRs-NiO based microfluidic sensor exhibits linearity in the range of 1.5-10.3 mM, a high sensitivity of 0.12 mA mM(-1) cm(-2) and a low value of 0.16 mM of the Michaelis-Menten constant (Km).
authors: Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
authors: Md Azahar
authors: MA
authors: Ali
authors: Pratima R
authors: PR
authors: Solanki
authors: Manoj K
authors: MK
authors: Patel
authors: Hemant
authors: H
authors: Dhayani
authors: Ved Varun
authors: VV
authors: Agrawal
authors: Renu
authors: R
authors: John
authors: Bansi D
authors: BD
authors: Malhotra
mesh_terms: D015374:Biosensing Techniques; D002789:Cholesterol Oxidase / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques; D043942:Nanotubes / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D002787:Sterol Esterase / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D009532:Nickel; C028007:nickel monoxide; D002789:Cholesterol Oxidase; D002787:Sterol Esterase