title: Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells.
abstract: A simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this trans-complementation system, it was found that nearly all of the capsid-coding sequence in the viral RNA was dispensable for the generation of pseudoinfectious dengue virus particles in mosquito cells.
authors: Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10700, Thailand.
authors: Sutha
authors: S
authors: Sangiambut
authors: Amporn
authors: A
authors: Suphatrakul
authors: Rungtawan
authors: R
authors: Sriburi
authors: Poonsook
authors: P
authors: Keelapang
authors: Chunya
authors: C
authors: Puttikhunt
authors: Watchara
authors: W
authors: Kasinrerk
authors: Prida
authors: P
authors: Malasit
authors: Nopporn
authors: N
authors: Sittisombut
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D009033:Culicidae; D003716:Dengue Virus / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D014162:Transfection; D019562:Viral Load; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins