title: Neuroinflammation and complexes of 17β-hydroxysteroid dehydrogenase type 10--amyloid β in Alzheimer's disease.
abstract: Multifunctional mitochondrial enzyme 17β-hydroxysteroid dehydrogenase type 10 plays a role in the development of Alzheimer's disease. However, changes in its expression in the brain or cerebrospinal fluid are not fully specific for this type of dementia. Our previous study revealed that complexes of the enzyme and amyloid β in cerebrospinal fluid could serve as a more specific biomarker of Alzheimer's disease than either the enzyme or amyloid β individually when compared to autoimmune multiple sclerosis. In this study, enzyme-linked immunosorbent assay and the surface plasmon resonance biosensor method were used to analyse cerebrospinal fluid of patients with various neuroinflammatory diseases. Significant differences in the levels of the total enzyme, complexes, amyloid β 1-42 and total τ/phospho-τ were found in Alzheimer's disease patients while differences in complexes, total amyloid β and amyloid β 1- 42 were observed in patients with neuroinflammatory diseases (except for multiple sclerosis) when compared to non-neuroinflammatory controls. The interactions of the enzyme with amyloid β appeared to depend strongly on neuroinflammation-sensitive amyloid β. Our data demonstrated that oligomerisation/aggregation of intracellular amyloid β peptides was important in Alzheimer's disease while extracellular amyloid β could play a role in neuroinflammatory diseases. Phospho-τ is currently the best biomarker of Alzheimer's disease.
authors: Prague Psychiatric Centre, Ústavní 91, 181 03 Prague 8, Czech Republic. kristofikova@pcp.lf3.cuni.cz
authors: Zdena
authors: Z
authors: Krištofiková
authors: Daniela
authors: D
authors: Řípová
authors: Aleš
authors: A
authors: Bartoš
authors: Markéta
authors: M
authors: Bocková
authors: Kateřina
authors: K
authors: Hegnerová
authors: Jan
authors: J
authors: Řičný
authors: Linda
authors: L
authors: Čechová
authors: Monika
authors: M
authors: Vrajová
authors: Jiří
authors: J
authors: Homola
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000134:cerebrospinal fluid*; D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000150:complications; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000150:complications; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation / Q000134:cerebrospinal fluid* / Q000150:complications; D008297:Male; D010446:Peptide Fragments / Q000134:cerebrospinal fluid*; D010523:Peripheral Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000150:complications; D018709:Statistics, Nonparametric; D020349:Surface Plasmon Resonance; D014652:Vascular Diseases / Q000134:cerebrospinal fluid / Q000150:complications
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase