title: Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing.
abstract: In eukaryotes, many genes are transcribed as precursor messenger RNAs (pre-mRNAs) that contain exons and introns, the latter of which must be removed and exons ligated to form the mature mRNAs. This process is called pre-mRNA splicing, which occurs in the nucleus. Although the chemistry of pre-mRNA splicing is identical to that of the self-splicing Group II introns, hundreds of proteins and five small nuclear RNAs (snRNAs), U1, U2, U4, U5, and U6, are essential for executing pre-mRNA splicing. Spliceosome, arguably the most complex cellular machine made up of all those proteins and snRNAs, is responsible for carrying out pre-mRNA splicing. In contrast to the transcription and the translation machineries, spliceosome is formed anew onto each pre-mRNA and undergoes a series of highly coordinated reconfigurations to form the catalytic center. This amazing process is orchestrated by a number of DExD/H-proteins that are the focus of this article, which aims to review the field in general and to project the exciting challenges and opportunities ahead. This article is part of a Special Issue entitled: The Biology of RNA helicases - Modulation for life.
authors: Genomics Research Center, Academia Sinica, Taipei, Taiwan. chang108@gate.sinica.edu.tw
authors: Tien-Hsien
authors: TH
authors: Chang
authors: Luh
authors: L
authors: Tung
authors: Fu-Lung
authors: FL
authors: Yeh
authors: Jui-Hui
authors: JH
authors: Chen
authors: Shang-Lin
authors: SL
authors: Chang
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D012326:RNA Splicing / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D015003:Yeasts / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D053487:DEAD-box RNA Helicases