title: Effects of conjugated linoleic acids on prostaglandin secretion by bovine endometrial epithelial cells in vitro.
abstract: OBJECTIVE
  To determine the effects of 2 conjugated linoleic acid (CLA) isomers (cis-9, trans-11 and trans-10, cis-12) on synthesis of prostaglandin (PG) E(2) and F(2α) and expression of prostaglandin H synthase-2 (PGHS-2) of adult and fetal bovine endometrial epithelial cells in vitro.
  
  
  METHODS
  Primary cultures of endometrial epithelial cells obtained from 4 adult cows and 4 fetal bovine carcasses.
  
  
  METHODS
  Cells were exposed to 0, 50, 100, or 200μM cis-9, trans-11 or trans-10, cis-12 CLA isomers for 24 hours. Culture media collected before and after 6 hours of stimulation of cells with phorbol 12-myristate 13-acetate were assayed to detect PGE(2) and PGF(2α) via ELISA. After stimulation, cells were collected for western blot analysis to quantify PGHS-2.
  
  
  RESULTS
  Concentrations of PGF(2α) and PGE(2) were significantly lower in culture media of adult and fetal endometrial epithelial cells exposed to any concentration of either CLA than they were in media of cells not exposed to CLAs. The trans-10, cis-12 CLA isomer seemed to decrease PG production more markedly than did the cis-9, trans-11 CLA isomer. Most concentrations of both CLAs significantly reduced culture media PGE(2):PGF(2α) concentration ratios of cells. Exposure of cells to CLAs did not affect expression of PGHS-2 protein.
  
  
  CONCLUSIONS
  Results of this study indicated CLAs significantly decreased PGF(2α) and PGE(2) concentrations and PGE(2):PGF(2α) concentration ratios for cultures of adult and fetal endometrial epithelial cells with no apparent effect on PGHS-2 expression. Similar effects in cows could have effects on maternal recognition of pregnancy and immune function.
authors: Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad 91775-1163, Iran.
authors: Alireza
authors: A
authors: Heravi Moussavi
authors: W Ronald
authors: WR
authors: Butler
authors: Dale E
authors: DE
authors: Bauman
authors: Robert O
authors: RO
authors: Gilbert
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015237:Dinoprost / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D011247:Pregnancy
chemical_list: D044243:Linoleic Acids, Conjugated; D015237:Dinoprost; D051546:Cyclooxygenase 2; D015232:Dinoprostone