title: Micro-optical lens array for fluorescence detection in droplet-based microfluidics.
abstract: We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals. We show that the integration of microlenses and mirror surfaces in these devices results in an 8-fold increase in the fluorescence signal and in improved spatial resolution. Using an array of microlenses, massively parallel detection of droplets containing fluorescent dyes was achieved, leading to detection throughputs of about 2000 droplets per second and per lens, parallelized over 625 measurement points.
authors: Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Goettingen, Germany.
authors: Jiseok
authors: J
authors: Lim
authors: Philipp
authors: P
authors: Gruner
authors: Manfred
authors: M
authors: Konrad
authors: Jean-Christophe
authors: JC
authors: Baret
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D007909:Lenses; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009821:Oils / Q000737:chemistry; D055096:Optical Devices
chemical_list: D005456:Fluorescent Dyes; D009821:Oils