title: A proteoliposome formulation derived from Bordetella pertussis induces protection in two murine challenge models.
abstract: Whooping cough remains a health problem despite high vaccination coverage. It has been recommended that development of new strategies provide long-lasting immunity. The aim of this work was to evaluate the potential of proteoliposomes (PL) extracted from Bordetella pertussis as a vaccine candidate against whooping cough. The size of the B. pertussis PL was estimated to be 96.7 ± 50.9 nm by Scanning Correlation Spectroscopy and the polydispersity index was 0.268. Western blots using monoclonal antibodies revealed the presence of pertussis toxin, pertactin, and fimbriae 3. The Limulus Amebocyte Lisate (LAL) assay showed endotoxin levels lower than those reported for whole cell pertussis licensed vaccines, while the Pyrogen Test indicated 75 ng/mL/Kg. The PL showed high protection capacity in mouse challenge models. There was 89.7% survival in the intracerebral challenge and total reduction of the number of CFU in the intranasal challenge. No significant differences (p > 0.05) were observed between mice immunized with B. pertussis PL and the Cuban DTwP vaccine, whichever challenge model used. These results encouraged us to continue the development of the B. pertussis PL as a component of a new combined vaccine formulated with tetanus and diphtheria toxoids or as a booster dose for adolescents and adults.
authors: Research & Development Vice-presidency, Finlay Institute, Havana, Cuba. sfernandez@finlay.edu.cu
authors: Sonsire
authors: S
authors: Fernández
authors: Esther M
authors: EM
authors: Fajardo
authors: Aleida
authors: A
authors: Mandiarote
authors: Gemma
authors: G
authors: Año
authors: Maria A
authors: MA
authors: Padrón
authors: Michel
authors: M
authors: Acosta
authors: Rubén A
authors: RA
authors: Cabrera
authors: Luis A
authors: LA
authors: Riverón
authors: Maydelis
authors: M
authors: Álvarez
authors: Kirenia
authors: K
authors: Blaín
authors: Mildrey
authors: M
authors: Fariñas
authors: Daniel
authors: D
authors: Cardoso
authors: Luis G
authors: LG
authors: García
authors: Concepción
authors: C
authors: Campa
authors: José L
authors: JL
authors: Pérez
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D005260:Female; D036781:Fimbriae Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin / Q000276:immunology; D011510:Proteolipids / Q000276:immunology*; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D011510:Proteolipids; D010566:Virulence Factors, Bordetella; C020485:proteoliposomes; D036781:Fimbriae Proteins; C068929:pertactin; D037342:Pertussis Toxin