title: Excitation polarization provides structural resolution of individual non-blinking nano-objects.
abstract: We propose to combine the method of fluorescence intensity centroid localization with rotation of the plane of excitation polarization. Polarized light interacts selectively with differently oriented fluorophores; thus yielding topological information on the nanometer scale, without any need for fluorophore blinking. The method is applicable to photostable individual systems, when most of the traditional super-resolution methods fail. A theoretical study is supported by experiments on 30 nm long cyclodextrin-encapsulated single polyrotaxane conjugated polymer chains.
authors: Chemical Physics, Lund University, Box 124, 22100, Lund, Sweden.
authors: Daniel
authors: D
authors: Thomsson
authors: Giuseppe
authors: G
authors: Sforazzini
authors: Harry L
authors: HL
authors: Anderson
authors: Ivan G
authors: IG
authors: Scheblykin