title: Exploring the dynamic core microbiome of plaque microbiota during head-and-neck radiotherapy using pyrosequencing.
abstract: Radiotherapy is the primary treatment modality used for patients with head-and-neck cancers, but inevitably causes microorganism-related oral complications. This study aims to explore the dynamic core microbiome of oral microbiota in supragingival plaque during the course of head-and-neck radiotherapy. Eight subjects aged 26 to 70 were recruited. Dental plaque samples were collected (over seven sampling time points for each patient) before and during radiotherapy. The V1-V3 hypervariable regions of bacterial 16S rRNA genes were amplified, and the high-throughput pyrosequencing was performed. A total of 140 genera belonging to 13 phyla were found. Four phyla (Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria) and 11 genera (Streptococcus, Actinomyces, Veillonella, Capnocytophaga, Derxia, Neisseria, Rothia, Prevotella, Granulicatella, Luteococcus, and Gemella) were found in all subjects, supporting the concept of a core microbiome. Temporal variation of these major cores in relative abundance were observed, as well as a negative correlation between the number of OTUs and radiation dose. Moreover, an optimized conceptual framework was proposed for defining a dynamic core microbiome in extreme conditions such as radiotherapy. This study presents a theoretical foundation for exploring a core microbiome of communities from time series data, and may help predict community responses to perturbation as caused by exposure to ionizing radiation.
authors: Department of Endodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Yue-jian
authors: YJ
authors: Hu
authors: Zi-yang
authors: ZY
authors: Shao
authors: Qian
authors: Q
authors: Wang
authors: Yun-tao
authors: YT
authors: Jiang
authors: Rui
authors: R
authors: Ma
authors: Zi-sheng
authors: ZS
authors: Tang
authors: Zheng
authors: Z
authors: Liu
authors: Jing-ping
authors: JP
authors: Liang
authors: Zheng-wei
authors: ZW
authors: Huang
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D004307:Dose-Response Relationship, Radiation; D014644:Genetic Variation; D006258:Head and Neck Neoplasms / Q000382:microbiology* / Q000532:radiotherapy*; D006801:Humans; D054892:Metagenome / Q000235:genetics*; D008875:Middle Aged; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature; D013997:Time Factors