title: Analysis of genomic aberrations associated with the clinicopathological parameters of rectal cancer by array‑based comparative genomic hybridization.
abstract: The aim of the present study was to screen and identify the chromosomal aberrations that are correlated with clinicopathological characteristics of rectal cancer using array-based comparative genomic hybridization (array-CGH). Forty-eight fresh frozen tumor tissues of rectal carcinoma were analyzed by array-CGH. The results showed that most frequent gains included 8q24.3, 20q11.21-q13.32, 20q13.33 and losses in 8p23.3-p12, 17p13.1-p12 and 18q11.2-q23 were noted. Fourteen amplifications and seven homozygous deletions were identified in the rectal cancer samples. Losses of 4p16.1-p15.31, 8p21.1-p12 and gains of 7p12.3-p12.1 and 13q33.1-q34 were associated with positive lymph node status and advanced clinical stage (stages III and IV). The 17q24.2-25.3 gain was more frequent in patients with distant metastasis. Integrated analysis indicated that overexpression of PDP1, TRIB1, C13orf27, FOXA2, PMEPA1 and PHACTR3 was associated with gains, and underexpression of FHOD, SMAD4 and BCL2 was associated with losses. Pathway enrichment analysis showed that pathways of nitrogen metabolism, oxidative phosphorylation, cell cycle, maturity onset diabetes of young, cytokine-cytokine receptor interaction, MAPK signaling pathway and dentatorubropallidoluysian atrophy were influenced by copy number changes.
authors: Department of Abdominal Surgical Oncology, Cancer Hospital and Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
authors: Jian-Wei
authors: JW
authors: Liang
authors: Zhi-Zhou
authors: ZZ
authors: Shi
authors: Tong-Tong
authors: TT
authors: Zhang
authors: Jia-Jie
authors: JJ
authors: Hao
authors: Zheng
authors: Z
authors: Wang
authors: Xiao-Min
authors: XM
authors: Wang
authors: Hai
authors: H
authors: Yang
authors: Ming-Rong
authors: MR
authors: Wang
authors: Zhi-Xiang
authors: ZX
authors: Zhou
authors: Yu
authors: Y
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D001284:Atrophy / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization / Q000379:methods; D016207:Cytokines / Q000235:genetics; D056915:DNA Copy Number Variations; D005260:Female; D018628:Gene Dosage; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism; D010766:Phosphorylation; D012004:Rectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015398:Signal Transduction / Q000235:genetics; D055815:Young Adult
chemical_list: D016207:Cytokines; D020928:Mitogen-Activated Protein Kinases; D009584:Nitrogen