title: Replacement of the heterologous 5' untranslated region allows preservation of the fully functional activities of type 2 porcine reproductive and respiratory syndrome virus.
abstract: The 5' untranslated region (UTR) is believed to be vital for the replication of porcine reproductive and respiratory syndrome virus (PRRSV), yet its functional mechanism remains largely unknown. In this study, to define the cis-acting elements for viral replication and infectivity, The 5' UTR swapping chimeric clones pTLV8 and pSHSP5 were constructed based on two different genotypes full-length infectious cDNA clone pAPRRS and pSHE backbones. Between them, vTLV8 could be rescued from pTLV8 and had similar virological properties to vAPRRS, including phenotypic characteristic and RNA synthesis level. However, pSHSP5 exhibited no evidence of infectivity. Taken together, the results presented here demonstrate that only the 5' UTR of type 1 PRRSV did not affect the infectivity and replication of type 2 PRRSV in vitro. The 5' UTR of type 2 PRRSV could be functionally replaced by its counterpart from type 1.
authors: Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Fei
authors: F
authors: Gao
authors: Huochun
authors: H
authors: Yao
authors: Jiaqi
authors: J
authors: Lu
authors: Zuzhang
authors: Z
authors: Wei
authors: Haihong
authors: H
authors: Zheng
authors: Jinshan
authors: J
authors: Zhuang
authors: Guangzhi
authors: G
authors: Tong
authors: Shishan
authors: S
authors: Yuan
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D016415:Sequence Alignment; D013552:Swine; D019562:Viral Load; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral