title: Antiseptic cyclodextrin-functionalized hydrogels and gauzes for loading and delivery of benzalkonium chloride.
abstract: Prevention and management of wound infections receive a lot of attention, since the presence of micro-organisms interferes with the wound-healing process. The aim of this work was to use cyclodextrins (CDs) to endow hydrogels and gauzes with the ability to take up antiseptics and sustain their delivery for several hours. Benzalkonium chloride (BzCl) can form inclusion complexes with cross-linked CDs that regulate the release through an affinity-driven mechanism. Grafting of CDs to cotton gauzes using citric acid as the linker, at 190 °C and for 15 min, led to grafting yields of about 148%, much larger than those obtained at 180 °C or with shorter reaction times. Microbiological tests revealed that the BzCl-loaded networks can inhibit the growth of Staphylococcus epidermidis and Escherichia coli both on agar plates and in liquid medium. Furthermore, the antiseptic-loaded gauzes were able to inhibit biofilm formation by Staphylococcus aureus RN1HG pMV158GFP when applied in early stages of biofilm formation and could reduce the number of living cells in preformed biofilms grown in a chronic wound biofilm model. These findings highlight the role of CDs as main components of hydrogels and gauzes for the efficient delivery of antiseptics.
authors: Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
authors: Maria José
authors: MJ
authors: Garcia-Fernandez
authors: Gilles
authors: G
authors: Brackman
authors: Tom
authors: T
authors: Coenye
authors: Angel
authors: A
authors: Concheiro
authors: Carmen
authors: C
authors: Alvarez-Lorenzo
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology*; D001458:Bandages; D001548:Benzalkonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D019343:Citric Acid / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D001548:Benzalkonium Compounds; D020100:Hydrogels; D047392:beta-Cyclodextrins; D049452:Green Fluorescent Proteins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D019343:Citric Acid