title: Protective role of AQP3 in UVA-induced NHSFs apoptosis via Bcl2 up-regulation.
abstract: Aquaporin-3 (AQP3), a water/glycerol-transporting protein that facilitates water, urea, and glycerol transport, can inhibit arsenite-induced apoptosis by up-regulating Bcl-2. However, whether it has a protective role in ultraviolet A (UVA)-induced apoptosis in normal human skin fibroblasts is not known. In this study, we demonstrate that mild UVA treatment fails to induce oxidative cell stress and apoptosis in normal human skin fibroblasts (NHSFs) overexpressing AQP3. After severe UVA irradiation, there was an increase in oxidative cell stress and apoptosis when AQP3 levels decreased. We also found that silencing AQP3 sensitized NHSFs to low-dose UVA. Overexpressing AQP3 was protective against high-dose UVA-induced oxidative stress and apoptosis. Besides, we observed that Bcl-2 may be involved in UVA-induced apoptosis. Our findings suggested that the water/glycerol-transporting protein AQP3 plays a role in resistance to UVA-induced apoptosis.
authors: Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors: Hongfu
authors: H
authors: Xie
authors: Fangfen
authors: F
authors: Liu
authors: Leishan
authors: L
authors: Liu
authors: Jian
authors: J
authors: Dan
authors: Ying
authors: Y
authors: Luo
authors: Yuxin
authors: Y
authors: Yi
authors: Xiang
authors: X
authors: Chen
authors: Ji
authors: J
authors: Li
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002648:Child; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000528:radiation effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D034622:RNA Interference; D011836:Radiation Tolerance; D015398:Signal Transduction / Q000528:radiation effects; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014162:Transfection; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: C496027:AQP3 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051400:Aquaporin 3