title: ORGANIC-HIGH IONIC STRENGTH AQUEOUS SOLVENT SYSTEMS FOR SPIRAL COUNTER-CURRENT CHROMATOGRAPHY: GRAPHIC OPTIMIZATION OF PARTITION COEFFICIENT.
abstract: A new series of organic-high ionic strength aqueous two-phase solvents systems was designed for separation of highly polar compounds by spiral high-speed counter-current chromatography. A total of 21 solvent systems composed of 1-butanol-ethanol-saturated ammonium sulfate-water at various volume ratios are arranged according to an increasing order of polarity. Selection of the two-phase solvent system for a single compound or a multiple sample mixture can be achieved by two steps of partition coefficient measurements using a graphic method. The capability of the method is demonstrated by optimization of partition coefficient for seven highly polar samples including tartrazine (K=0.77), tryptophan (K=1.00), methyl green (K= 0.93), tyrosine (0.81), metanephrine (K=0.89), tyramine (K=0.98), and normetanephrine (K=0.96). Three sulfonic acid components in D&C Green No. 8 were successfully separated by HSCCC using the graphic selection of the two-phase solvent system.
authors: Bioseparation Technology Laboraotry, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Bldg. 10, Rm 8N230, DhBethesda, M.D., USA ; Institute of Materia Medica and Department of Pharmacology, North Sichuan Medical College, Nanchong, 63007, Sichuan Province, China.
authors: Yun
authors: Y
authors: Zeng
authors: Gang
authors: G
authors: Liu
authors: Ying
authors: Y
authors: Ma
authors: Xiaoyuan
authors: X
authors: Chen
authors: Yoichiro
authors: Y
authors: Ito
keywords: Catecholamines; Graphic optimization of partition coefficient; Organic-high ionic strength aqueous solvent system series; Spiral high-speed counter-current chromatography; Sulfonic acids; Zwitter ions