title: Determination of dioxin-like polychlorinated biphenyls in 1 mL whole blood using programmable temperature vaporization large volume injection coupled to gas chromatogram and high-resolution mass spectrometry.
abstract: A sensitive method based on programmable temperature vaporization large volume injection coupled to gas chromatogram and high-resolution mass spectrometry (PTV-GC-HRMS) has been developed for the determination of ultra trace levels of dioxin-like polychlorinated biphenyls (DL PCBs) in small amounts of human blood. Blood samples (1mL) were first extracted by column extraction and then purified with column chromatorgraphies. Final extracts (20μL) were introduced to the PTV injector under the solvent vent mode and detected by GC-HRMS (SIM mode). PTV parameters were observed by changing one factor at a time (practical conditions: vent flow: 50mLmin(-1), vent pressure: 0kPa and vent time: 0.1min), recoveries of most PCB congeners ranged from 55.1% to 108%, and method detection limits were in the range of 0.11-1.63pgg(-1).
authors: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center For Food Safety Risk Assessment, 7 Panjiayuan Nanli, 100021 Beijing, China.
authors: Haitao
authors: H
authors: Shen
authors: Rongfa
authors: R
authors: Guan
authors: Jingguang
authors: J
authors: Li
authors: Lei
authors: L
authors: Zhang
authors: Yiping
authors: Y
authors: Ren
authors: Xiaomin
authors: X
authors: Xu
authors: Yang
authors: Y
authors: Song
authors: Yunfeng
authors: Y
authors: Zhao
authors: Jianlong
authors: J
authors: Han
authors: Yongning
authors: Y
authors: Wu
mesh_terms: D002849:Chromatography, Gas / Q000295:instrumentation; D004147:Dioxins / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation; D011078:Polychlorinated Biphenyls / Q000097:blood*; D012680:Sensitivity and Specificity; D013696:Temperature; D014835:Volatilization
chemical_list: D004147:Dioxins; D011078:Polychlorinated Biphenyls