title: Genotoxicity assessment of vaccine adjuvant squalene.
abstract: The genotoxic potential of the vaccine adjuvant Squalene was assessed by the chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and micronucleus (MNs) tests in human lymphocytes and comet assay in both human and rat lymphocytes. Five different concentrations of squalene (1250-20,000 μg/ml for human lymphocytes and 0.07-1.12 mg/kg for rat lymphocytes) were studied. Squalene did not affect the CAs and MN frequency, in all treatments in vitro. A significant increase in SCEs was observed in almost all concentrations at 24 h treatment. Squalene did not affect significantly the comet tail length (CTL) (except 2500 μg/ml) and comet tail intensity (CTI) at all treatments in vitro. In rats, squalene significantly increased and decreased CTL and CTI in some doses. Although there are increasing and reduction in the effect, squalene cannot be regarded as genotoxic in human lymphocytes. However, further in vivo studies are required to be sure on the effect.
authors: Faculty of Science, Department of Biology, Genetic Toxicology Laboratory, Gazi University, 06500 Teknikokullar, Ankara, Turkey. deniz@gazi.edu.tr
authors: D
authors: D
authors: Yüzbaşıoğlu
authors: F
authors: F
authors: Ünal
authors: F
authors: F
authors: Koç
authors: S
authors: S
authors: Öztemel
authors: H
authors: H
authors: Aksoy
authors: S
authors: S
authors: Mamur
authors: F
authors: F
authors: Demirtaş Korkmaz
mesh_terms: D000276:Adjuvants, Immunologic / Q000009:adverse effects*; D000328:Adult; D000818:Animals; D002869:Chromosome Aberrations / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D015162:Micronucleus Tests; D051381:Rats; D017208:Rats, Wistar; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013185:Squalene / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D000276:Adjuvants, Immunologic; D013185:Squalene