title: The delivery of thrombi-specific nanoparticles incorporating oligonucleotides into injured cerebrovascular endothelium.
abstract: In acute vascular events, the endothelium derived tissue factor (TF) is the trigger of the coagulation cascade. In this study, EGFP-EGF1 protein-conjugated PEG-PLGA nanoparticle was employed as a TF targeting vehicle, the NF-κB decoy oligonucleotides (ODNs) was incorporated into it and the resulting EGF1-EGFP-NP-ODNs were evaluated as a vector for therapy of cortex infarction. At 2 h after transfection of TF expressed rat brain capillary endothelial cell, EGF1-EGFP-NP-ODNs was more efficiently internalized and located in the cytoplasm than NP-ODNs. At 4 h and 6 h after administration, ODNs were present in the nuclei and obviously inhibited the TF expression. At 6 h after i.v. administration in vivo, most EGF1-EGFP-NP were accumulated in the embolism vessels, distributed in the damaged endothelial cells and lowered the TF expression. At 24 h after i.v. administration, MR imaging of cortex infarcts were predominantly dwindled.
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China.
authors: Wei
authors: W
authors: Shi
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China.
authors: Heng
authors: H
authors: Mei
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China.
authors: Jun
authors: J
authors: Deng
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China.
authors: Chen
authors: C
authors: Chen
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China.
authors: Huafang
authors: H
authors: Wang
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China.
authors: Tao
authors: T
authors: Guo
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China.
authors: Bo
authors: B
authors: Zhang
authors: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 200032, PR China.
authors: Zhiqing
authors: Z
authors: Pang
authors: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 200032, PR China.
authors: Xinguo
authors: X
authors: Jiang
authors: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, Hubei 430071, PR China.
authors: Xuxia
authors: X
authors: Wang
authors: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, Hubei 430071, PR China.
authors: Hao
authors: H
authors: Lei
authors: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China; Targeted Biotherapy Key Laboratory of Ministry of Education, Wuhan, Hubei 430022, PR China. Electronic address: dr_huyu@126.com.
authors: Yu
authors: Y
authors: Hu
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D016503:Drug Delivery Systems; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D005453:Fluorescence; D005786:Gene Expression Regulation / Q000187:drug effects; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D009841:Oligonucleotides / Q000494:pharmacology*; D010316:Particle Size; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D055672:Static Electricity; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism; D013927:Thrombosis / Q000378:metabolism / Q000473:pathology*
chemical_list: D009841:Oligonucleotides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D013925:Thromboplastin