title: Influence of a mutation in IFN-γ receptor 2 (IFNGR2) in human cells on the generation of Th17 cells in memory T cells.
abstract: The T cell subsets involved in inflammatory reactions are mainly the IFN-γ secreting Th1 cells and IL17-producing Th17 cells. Although Th17 cells are primed in the thymus, there is evidence that Th17 cells can be generated from effector memory CD4(+) T cells. Cytokines as IL-6, TGF-β, IL-21 and IL-23 involved in development of Th17 cells are well described. Here we analyzed the impact of a mutation in the IFN-γ receptor 2 (IFN-γR2) on the induction of Th17 cells. By isolation of T cells and monocytes of a patient with this mutation we could demonstrate an inhibitory role of IFN-γ signaling as IFN-γR2-deficient monocytes induce a higher percentage of IL-17(+) cells from both healthy and IFN-γR2-deficient CD4(+) T cells. This data confirm the interference of these two T helper subsets and points to a balance of Th1 and Th17 cells obtained by their own cytokine production and their interplay with APCs.
authors: Children's Hospital, University of Tuebingen, Tuebingen, Germany. ursula.holzer@med.uni-tuebingen.de
authors: Ursula
authors: U
authors: Holzer
authors: Katharina
authors: K
authors: Reinhardt
authors: Peter
authors: P
authors: Lang
authors: Rupert
authors: R
authors: Handgretinger
authors: Nadja
authors: N
authors: Fischer
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D015870:Gene Expression; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D020381:Interleukin-17 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009154:Mutation; D017471:Receptors, Interferon / Q000172:deficiency / Q000235:genetics*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism*; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D000096743:Interferon gamma Receptor
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor