title: Interleukin-6 -174 G/C promoter gene polymorphism in nasal polyposis and asthma.
abstract: BACKGROUND
  Interleukin-6 (IL-6) is an inflammatory mediator linked to nasal polyposis and asthma, with a single nucleotide poly- morphism -174 G/C that seems to promote an inflammatory status. We aimed to analyze the relationship between this poly-morhism and asthmatic nasal polyposis patients.
  
  
  METHODS
  Cross-sectional study to investigate IL-6 - 174 G/C genotypes of 45 nasal polyposis with asthma patients, 63 nasal polyposis-only patients, 45 asthma-only patients and 81 subjects without both diseases. Aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with specific primers followed by restriction enzyme analysis, classifying patients in GG, GC or CC genotype.
  
  
  RESULTS
  The GG genotype was the most frequent in all inflammatory groups. Less than 40% of controls presented with the GG ge- notype. There were significant differences between inflammatory groups and control group. No significant differences were seen when comparing inflammatory groups to each other, other than between nasal polyposis-only group and asthma-only group.
  
  
  CONCLUSIONS
  The IL-6 74 GG genotype was found more frequently in all inflammatory groups than in controls. This genotype could influence nasal polyposis and asthma, and seems to be more important in the latter.
authors: Department of Otorhinolaryngology, Sao Paulo Federal University, Sao Paulo, Brazil. edumacoto@uol.com.br
authors: E M
authors: EM
authors: Kosugi
authors: C M
authors: CM
authors: de Camargo-Kosugi
authors: E R
authors: ER
authors: Hirai
authors: J A
authors: JA
authors: Mendes-Neto
authors: L C
authors: LC
authors: Gregorio
authors: I D C
authors: ID
authors: Guerreiro-da-Silva
authors: L L M
authors: LL
authors: Weckx
mesh_terms: D000483:Alleles; D000704:Analysis of Variance; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D009298:Nasal Polyps / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D018709:Statistics, Nonparametric
chemical_list: D015850:Interleukin-6