title: Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.
abstract: Microbial physiology plays a crucial role in whole-cell biotransformation, especially for redox reactions that depend on carbon and energy metabolism. In this study, regio- and enantio-selective proline hydroxylation with recombinant Escherichia coli expressing proline-4-hydroxylase (P4H) was investigated with respect to its interconnectivity to microbial physiology and metabolism. P4H production was found to depend on extracellular proline availability and on codon usage. Medium supplementation with proline did not alter p4h mRNA levels, indicating that P4H production depends on the availability of charged prolyl-tRNAs. Increasing the intracellular levels of soluble P4H did not result in an increase in resting cell activities above a certain threshold (depending on growth and assay temperature). Activities up to 5-fold higher were reached with permeabilized cells, confirming that host physiology and not the intracellular level of active P4H determines the achievable whole-cell proline hydroxylation activity. Metabolic flux analysis revealed that tricarboxylic acid cycle fluxes in growing biocatalytically active cells were significantly higher than proline hydroxylation rates. Remarkably, a catalysis-induced reduction of substrate uptake was observed, which correlated with reduced transcription of putA and putP, encoding proline dehydrogenase and the major proline transporter, respectively. These results provide evidence for a strong interference of catalytic activity with the regulation of proline uptake and metabolism. In terms of whole-cell biocatalyst efficiency, proline uptake and competition of P4H with proline catabolism are considered the most critical factors.
authors: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.
authors: Francesco
authors: F
authors: Falcioni
authors: Lars M
authors: LM
authors: Blank
authors: Oliver
authors: O
authors: Frick
authors: Andreas
authors: A
authors: Karau
authors: Bruno
authors: B
authors: Bühler
authors: Andreas
authors: A
authors: Schmid
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D001692:Biological Transport; D002244:Carbon / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000502:physiology; D015870:Gene Expression; D006900:Hydroxylation; D002479:Inclusion Bodies / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D011392:Proline / Q000378:metabolism*; D064828:Prolyl Hydroxylases / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D011994:Recombinant Proteins; D002244:Carbon; D011392:Proline; D064828:Prolyl Hydroxylases