title: Patient-derived models of human breast cancer: protocols for in vitro and in vivo applications in tumor biology and translational medicine.
abstract: Research models that replicate the diverse genetic and molecular landscape of breast cancer are critical for developing the next-generation therapeutic entities that can target specific cancer subtypes. Patient-derived tumorgrafts, generated by transplanting primary human tumor samples into immune-compromised mice, are a valuable method to model the clinical diversity of breast cancer in mice, and are a potential resource in personalized medicine. Primary tumorgrafts also enable in vivo testing of therapeutics and make possible the use of patient cancer tissue for in vitro screens. Described in this unit are a variety of protocols including tissue collection, biospecimen tracking, tissue processing, transplantation, and three-dimensional culturing of xenografted tissue, which enable use of bona fide uncultured human tissue in designing and validating cancer therapies.
authors: Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA.
authors: Yoko S
authors: YS
authors: DeRose
authors: Keith M
authors: KM
authors: Gligorich
authors: Guoying
authors: G
authors: Wang
authors: Ann
authors: A
authors: Georgelas
authors: Paulette
authors: P
authors: Bowman
authors: Samir J
authors: SJ
authors: Courdy
authors: Alana L
authors: AL
authors: Welm
authors: Bryan E
authors: BE
authors: Welm
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D013048:Specimen Handling; D057170:Translational Research, Biomedical / Q000379:methods*; D014183:Transplantation, Heterologous