title: Continuous mode of carbon dioxide sequestration by C. sorokiniana and subsequent use of its biomass for hydrogen production by E. cloacae IIT-BT 08.
abstract: The present study investigated to find out the suitability of the CO2 sequestered algal biomass of Chlorella sorokiniana as substrate for the hydrogen production by Enterobacter cloacae IIT-BT 08. The maximum biomass productivity in continuous mode of operation in autotrophic condition was enhanced from 0.05 g L(-1) h(-1) in air to 0.11 g L(-1) h(-1) in 5% air-CO2 (v/v) gas mixture at an optimum dilution rate of 0.05 h(-1). Decrease in steady state biomass and productivity was less sensitive at higher dilution and found fitting with the model proposed by Eppley and Dyer (1965). Pretreated algal biomass of 10 g L(-1) with 2% (v/v) HCl-heat was found most suitable for hydrogen production yielding 9±2 mol H2 (kg COD reduced)(-1) and was found fitting with modified Gompertz equation. Further, hydrogen energy recovery in dark fermentation was significantly enhanced compared to earlier report of hydrogen production by biophotolysis of algae.
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors: Kanhaiya
authors: K
authors: Kumar
authors: Shantonu
authors: S
authors: Roy
authors: Debabrata
authors: D
authors: Das
mesh_terms: D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D057965:Carbon Sequestration / Q000502:physiology*; D002708:Chlorella / Q000378:metabolism* / Q000502:physiology; D002849:Chromatography, Gas; D016972:Enterobacter cloacae / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D013053:Spectrophotometry
chemical_list: D006859:Hydrogen
keywords: Algal biomass; Biohydrogen; CO(2) sequestration; Chlorella sorokiniana; E. cloacae IIT-BT 08