title: Caffeic acid treatment alters the extracellular adenine nucleotide hydrolysis in platelets and lymphocytes of adult rats.
abstract: This study evaluated the effects of caffeic acid on ectonucleotidase activities such as NTPDase (nucleoside triphosphate diphosphohydrolase), Ecto-NPP (nucleotide pyrophosphatase/phosphodiesterase), 5'-nucleotidase and adenosine deaminase (ADA) in platelets and lymphocytes of rats, as well as in the profile of platelet aggregation. Animals were divided into five groups: I (control); II (oil); III (caffeic acid 10 mg/kg); IV (caffeic acid 50 mg/kg); and V (caffeic acid 100 mg/kg). Animals were treated with caffeic acid diluted in oil for 30 days. In platelets, caffeic acid decreased the ATP hydrolysis and increased ADP hydrolysis in groups III, IV and V when compared to control (P<0.05). The 5'-nucleotidase activity was decreased, while E-NPP and ADA activities were increased in platelets of rats of groups III, IV and V (P<0.05). Caffeic acid reduced significantly the platelet aggregation in the animals of groups III, IV and V in relation to group I (P<0.05). In lymphocytes, the NTPDase and ADA activities were increased in all groups treated with caffeic acid when compared to control (P<0.05). These findings demonstrated that the enzymes were altered in tissues by caffeic acid and this compound decreased the platelet aggregation suggesting that caffeic acid should be considered a potentially therapeutic agent in disorders related to the purinergic system.
authors: Programa de Pós-Graduação em Ciências Biológicas, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil.
authors: Javed
authors: J
authors: Anwar
authors: Roselia Maria
authors: RM
authors: Spanevello
authors: Victor Camera
authors: VC
authors: Pimentel
authors: Jessié
authors: J
authors: Gutierres
authors: Gustavo
authors: G
authors: Thomé
authors: Andreia
authors: A
authors: Cardoso
authors: Daniela
authors: D
authors: Zanini
authors: Caroline
authors: C
authors: Martins
authors: Heloisa Einloft
authors: HE
authors: Palma
authors: Margarete Dulce
authors: MD
authors: Bagatini
authors: Jucimara
authors: J
authors: Baldissarelli
authors: Roberta
authors: R
authors: Schmatz
authors: Cláudio Alberto Martins
authors: CA
authors: Leal
authors: Pauline
authors: P
authors: da Costa
authors: Vera Maria
authors: VM
authors: Morsch
authors: Maria Rosa Chitolina
authors: MR
authors: Schetinger
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D002109:Caffeic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D011755:Pyrophosphatases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000227:Adenine Nucleotides; D002109:Caffeic Acids; D010727:Phosphoric Diester Hydrolases; D000243:Adenosine Deaminase; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; C027201:ectoATPase; C021899:nucleotide pyrophosphatase; C040048:caffeic acid