title: Female serum immunoglobulins G, A, E and their immunological reactions to seminal fluid antigens.
abstract: One in five couples of reproductive age has           been diagnosed with infertility. Some diagnoses indicate           an immunological basis for this disorder. Female           immune infertility may be caused by iso-immunization           by seminal components. We focused on the characterization           of seminal proteins to illustrate the IgG,           IgA and IgE immune responses of 31 infertile women.           The biochemical characterization was performed           by one-dimensional sodium dodecyl sulphate polyacrylamide           gel electrophoresis and isoelectric focusing,           both of which were followed by immunoblotting           analyses. IgG mainly recognized the antigens with           relative molecular masses (Mr) 95 and 183 kDa and           isoelectric points ranging from 6.9 to 7.0. The immunodominant           antigens recognized by IgA had the Mr           of 35 kDa and isoelectric points ranging from 6.2 to           7.2. The reactivity of IgE was not confirmed within           our group of patients. The seminal IgG- and IgA           -binding patterns were analysed immunochemically           to determine the characteristics of possible seminal           proteins associated with female immune infertility.
authors: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic. andrea.brazdova@vscht.cz
authors: A
authors: A
authors: Brázdová
authors: J
authors: J
authors: Zídková
authors: H
authors: H
authors: Senechal
authors: G
authors: G
authors: Peltre
authors: J
authors: J
authors: Cibulka
authors: Z
authors: Z
authors: Ulčová-Gallová
mesh_terms: D000328:Adult; D000941:Antigens / Q000276:immunology*; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007247:Infertility, Female / Q000276:immunology; D007525:Isoelectric Focusing; D012661:Semen / Q000276:immunology*
chemical_list: D000941:Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007073:Immunoglobulin E