title: Development and characterization of the replicon system of Japanese encephalitis live vaccine virus SA14-14-2.
abstract: BACKGROUND
  Viral self-replicating sub-genomic replicons represent a powerful tool for studying viral genome replication, antiviral screening and chimeric vaccine development. Many kinds of flavivirus replicons have been developed with broad applications.
  
  
  RESULTS
  The replicon system of JEV live vaccine strain SA14-14-2 was successfully developed in this study. Two kinds of replicons that express enhanced green fluorescent protein (EGFP) and Renilla luciferase (R.luc) were constructed under the control of SP6 promoter, respectively. Robust EGFP and R.luc signals could be detected in the replicon-transfected BHK-21 cells. Furthermore, the potential effects of selected amino acids in the C-terminal of envelope protein on replication were characterized using the replicon system.
  
  
  CONCLUSIONS
  Our results provide a useful platform not only for the study of JEV replication, but also for antiviral screening and chimeric vaccine development.
authors: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
authors: Shi-Hua
authors: SH
authors: Li
authors: Xiao-Feng
authors: XF
authors: Li
authors: Hui
authors: H
authors: Zhao
authors: Yong-Qiang
authors: YQ
authors: Deng
authors: Xue-Dong
authors: XD
authors: Yu
authors: Shun-Ya
authors: SY
authors: Zhu
authors: Tao
authors: T
authors: Jiang
authors: Qing
authors: Q
authors: Ye
authors: E-De
authors: ED
authors: Qin
authors: Cheng-Feng
authors: CF
authors: Qin
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology; D004672:Encephalitis, Japanese / Q000517:prevention & control / Q000821:virology*; D006801:Humans; D022321:Japanese Encephalitis Vaccines / Q000235:genetics* / Q000276:immunology; D012093:Replicon
chemical_list: D022321:Japanese Encephalitis Vaccines