title: PKCα and PKCβ cooperate functionally in CD3-induced de novo IL-2 mRNA transcription.
abstract: The physiological functions of PKCα and PKCθ isotypes downstream of the antigen receptor have been defined in CD3(+) T cells. In contrast, no function of the second conventional PKC member, PKCβ, has been described yet in T cell antigen receptor signalling. To investigate the hypothesis that both conventional PKCα and PKCβ isotypes may have overlapping functions in T cell activation signalling, we generated mice that lacked the genes for both isotypes. We found that PKCα(-/-)/β(-/-) animals are viable, live normal life spans and display normal T cell development. However, these animals possess additive defects in T cell responses in comparison to animals that carry single mutations in these genes. Our studies demonstrate that the activities of PKCα and PKCβ converge to regulate IL-2 cytokine responses in anti-CD3 stimulated primary mouse T cells. Here, we present genetic evidence that PKCα and PKCβ cooperate in IL-2 transcriptional transactivation in primary mouse T cells independently of the actions of PKCθ.
authors: Christina
authors: C
authors: Lutz-Nicoladoni
authors: Tyrolean Cancer Reserach Institute (TKFI), Austria.
authors: Lutz-Nicoladoni
authors: LN
authors: Christina
authors: Nikolaus
authors: N
authors: Thuille
authors: Thuille
authors: T
authors: Nikolaus
authors: Katarzyna
authors: K
authors: Wachowicz
authors: Wachowicz
authors: W
authors: Katarzyna
authors: Thomas
authors: T
authors: Gruber
authors: Gruber
authors: G
authors: Thomas
authors: Michael
authors: M
authors: Leitges
authors: Leitges
authors: L
authors: Michael
authors: Gottfried
authors: G
authors: Baier
authors: Baier
authors: B
authors: Gottfried
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000276:immunology* / Q000378:metabolism; D007156:Immunologic Memory; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000378:metabolism; D010641:Phenotype; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D017252:CD3 Complex; D007376:Interleukin-2; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D018808:Transcription Factor AP-1; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha