title: High frequency shoots regeneration for mass multiplication of Phyllanthus fraternus Webster--an important antiviral and hepatoprotective plant.
abstract: An efficient, rapid, and highly reproducible regeneration protocol was successfully developed for Phyllanthus fraternus from the field-derived mature nodal segments. The explants induced multiple shoots on cytokinin containing medium. The highest frequency (99 %) and maximum number of shoots (19.75) were induced on Murashige and Skoog's (MS) medium supplemented with 2.22 μM 6-benzylaminopurine after 3-4 weeks of culture initiation. The elongated shoots were rooted on MS medium supplemented with indol-3-butyric acid (IBA) or α-naphthalene acetic acid. Pulse treatment of microshoots promoted significant increase in the percentage of rooting and number of root regeneration per shoot. The highest rooting (100 %) and maximum number of roots (8.75) per shoot was obtained when shoots were dipped in IBA solution (0.98 mM) for 5 min and further subcultured on MS basal medium. Plantlets were successfully acclimatized and established in soil. Regenerated plants were grown normally in the field without showing any morphological variations. This cost-effective protocol will help the mass multiplication of P. fraternus for commercial propagation and high biomass production of this valuable medicinal plant.
authors: Department of Botany, MMV, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India. ru4004@gmail.com
authors: Richa
authors: R
authors: Upadhyay
authors: Kavindra Nath
authors: KN
authors: Tiwari
authors: Karuna
authors: K
authors: Singh
mesh_terms: D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D003583:Cytokinins / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D029803:Phyllanthus / Q000187:drug effects / Q000254:growth & development*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D012038:Regeneration / Q000187:drug effects
chemical_list: D003470:Culture Media; D003583:Cytokinins; D007211:Indoles; D009280:Naphthaleneacetic Acids; C034182:1-naphthaleneacetic acid; C008122:indol-3-yl pyruvic acid