title: Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia.
abstract: OBJECTIVE
  Idiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study.
  
  
  METHODS
  Sperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.
  
  
  RESULTS
  Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent.
  
  
  CONCLUSIONS
  These experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia.
authors: Department of Andrology, Liuzhou Hospital of Traditional Chinese Medicine, Liuzhou, 545001, China.
authors: Shulin
authors: S
authors: Shen
authors: Jinzi
authors: J
authors: Wang
authors: Jihong
authors: J
authors: Liang
authors: Dalin
authors: D
authors: He
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D053627:Asthenozoospermia / Q000235:genetics* / Q000503:physiopathology*; D016022:Case-Control Studies; D000091342:Endoplasmic Reticulum Chaperone BiP; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics* / Q000502:physiology; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000235:genetics / Q000378:metabolism; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D000071617:Protein Deglycase DJ-1; D040901:Proteomics; D000072260:RNA Splicing Factors; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013081:Sperm Motility / Q000235:genetics* / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D000072260:RNA Splicing Factors; D011993:Recombinant Fusion Proteins; C518957:SPANXB1 protein, human; D014157:Transcription Factors; C417244:XAB2 protein, human; C410468:complement activation blocker 2, human; D005408:Flagellin; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D010735:Phosphoglycerate Kinase; C104317:phosphoglycerate kinase, testis specific; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1