title: [Establishment of a mouse model bearing spinal metastatic human prostate cancer using fluorescence-labeled PC-3 cells].
abstract: OBJECTIVE
  To establish a mouse model of spinal metastasis of human prostate cancer using fluorescence-labeled PC-3 cells to allow direct observation by in vivo imaging.
  
  
  METHODS
  PC-3 cells were infected with a lentivirus carrying green fluorescence protein (GFP) gene. The GFP-positive cell clone was expanded and prepared into cell suspension for injection into the inferior vena cava of nude mice. The tumor growth and metastasis in the mice was directly observed using an in vivo fluorescence imaging system. The tumor-bearing mice were sacrificed after 3 months for histological examination with HE staining.
  
  
  RESULTS
  The labeled cells showed stable GFP expression both in vitro and in vivo. One week after cell injection, green fluorescence signals were detected by the in vivo fluorescence imaging system in the lower back of the mice, and at 4 weeks, the fluorescent tumor mass increased with a bone metastasis rate of 19% (3/16). Dissection of the mice at 3 months revealed lumbar tumor infiltration in 3 mice, showing a consistent result with in vivo fluorescence imaging.
  
  
  CONCLUSIONS
  The nude mouse model of spinal bone metastasis of human prostate cancer established using GFP-labeled PC-3 cells facilitates further study of bone metastasis of prostate cancer.
authors: Department of Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning, China. cuimingxing1226@163.com
authors: Mingxing
authors: M
authors: Cui
authors: Xinli
authors: X
authors: Zhan
authors: Huijiang
authors: H
authors: Liu
authors: Juliang
authors: J
authors: He
authors: Ronghe
authors: R
authors: Gu
authors: Shengsheng
authors: S
authors: Huang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000473:pathology*; D013125:Spinal Neoplasms / Q000556:secondary*
chemical_list: D049452:Green Fluorescent Proteins