title: Efficient pro-survival/angiogenic miRNA delivery by an MRI-detectable nanomaterial.
abstract: Herein, we report the use of biodegradable nanoparticles (NPs) containing perfluoro-1,5-crown ether (PFCE), a fluorine-based compound (NP170-PFCE) with the capacity to track cells in vivo by magnetic ressonance imaging (MRI) and efficiently release miRNA. NP170-PFCE complexed with miRNAs accumulate whitin the cell's endolysosomal compartment and interact with higher frequency with argonaute2 (Ago2) and GW182 proteins, which are involved in the biological action of miRNAs, than commercial complexes formed by commercial reagents and miRNA, which in turn accumulate in the cell cytoplasm. The release of miRNA132 (miR132) from the NPs increased 3-fold the survival of endothelial cells (ECs) transplanted in vivo and 3.5-fold the blood perfusion in ischemic limbs relatively to control.
authors: CNC, Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors: Renata S M
authors: RS
authors: Gomes
authors: Ricardo Pires
authors: RP
authors: das Neves
authors: Lowri
authors: L
authors: Cochlin
authors: Ana
authors: A
authors: Lima
authors: Rui
authors: R
authors: Carvalho
authors: Petra
authors: P
authors: Korpisalo
authors: Galina
authors: G
authors: Dragneva
authors: Mikko
authors: M
authors: Turunen
authors: Timmo
authors: T
authors: Liimatainen
authors: Kieran
authors: K
authors: Clarke
authors: Seppo
authors: S
authors: Ylä-Herttuala
authors: Carolyn
authors: C
authors: Carr
authors: Lino
authors: L
authors: Ferreira
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D058948:Cell Tracking / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology; D006614:Hindlimb / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000473:pathology* / Q000628:therapy*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D035683:MicroRNAs / Q000008:administration & dosage* / Q000235:genetics*; D053769:Nanocapsules / Q000737:chemistry / Q000627:therapeutic use*; D014162:Transfection / Q000379:methods
chemical_list: D043925:Angiogenesis Inducing Agents; D035683:MicroRNAs; D053769:Nanocapsules