title: Tenderization effect of cold-adapted collagenolytic protease MCP-01 on beef meat at low temperature and its mechanism.
abstract: The enzymes currently used to increase meat tenderness are all mesophilic or thermophilic proteases. This study provides insight into the tenderization effect and the mechanism of a cold-adapted collagenolytic enzyme MCP-01 on beef meat at low temperatures. MCP-01 (10 U of caseinolytic activity) reduced the meat shear force by 23% and increased the relative myofibrillar fragmentation index of the meat by 91.7% at 4 °C, and it also kept the fresh colour and moisture of the meat. Compared to the commercially used tenderizers papain and bromelain, MCP-01 showed a unique tenderization mechanism. MCP-01 had a strong selectivity for degrading collagen at 4 °C, showed a distinct digestion pattern on the myofibrillar proteins, and had a different disruption pattern on the muscle fibres under scanning electron micrograph. These results suggest that the cold-adapted collagenolytic protease MCP-01 may be promising for use as a meat tenderizer at low and moderate temperatures.
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China.
authors: Guo-Yan
authors: GY
authors: Zhao
authors: Ming-Yang
authors: MY
authors: Zhou
authors: Hui-Lin
authors: HL
authors: Zhao
authors: Xiu-Lan
authors: XL
authors: Chen
authors: Bin-Bin
authors: BB
authors: Xie
authors: Xi-Ying
authors: XY
authors: Zhang
authors: Hai-Lun
authors: HL
authors: He
authors: Bai-Cheng
authors: BC
authors: Zhou
authors: Yu-Zhong
authors: YZ
authors: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D003094:Collagen / Q000737:chemistry*; D004338:Drug Combinations; D010450:Endopeptidases / Q000737:chemistry*; D005511:Food Handling / Q000295:instrumentation / Q000379:methods*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010206:Papain / Q000737:chemistry*; D012982:Sodium, Dietary
chemical_list: D004338:Drug Combinations; D012982:Sodium, Dietary; C032508:meat tenderizer; D003094:Collagen; D010450:Endopeptidases; C477696:protease MCP-01, Pseudoaltermonas; D010206:Papain