title: The Drosophila splicing factor PSI is phosphorylated by casein kinase II and tousled-like kinase.
abstract: Alternative splicing of pre-mRNA is a highly regulated process that allows cells to change their genetic informational output. These changes are mediated by protein factors that directly bind specific pre-mRNA sequences. Although much is known about how these splicing factors regulate pre-mRNA splicing events, comparatively little is known about the regulation of the splicing factors themselves. Here, we show that the Drosophila splicing factor P element Somatic Inhibitor (PSI) is phosphorylated at at least two different sites by at minimum two different kinases, casein kinase II (CK II) and tousled-like kinase (tlk). These phosphorylation events may be important for regulating protein-protein interactions involving PSI. Additionally, we show that PSI interacts with several proteins in Drosophila S2 tissue culture cells, the majority of which are splicing factors.
authors: Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.
authors: J Matthew
authors: JM
authors: Taliaferro
authors: Dhruv
authors: D
authors: Marwha
authors: Julie L
authors: JL
authors: Aspden
authors: Daniela
authors: D
authors: Mavrici
authors: Nathalie E
authors: NE
authors: Cheng
authors: Lori A
authors: LA
authors: Kohlstaedt
authors: Donald C
authors: DC
authors: Rio
mesh_terms: D000818:Animals; D047390:Casein Kinase II / Q000302:isolation & purification / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D013058:Mass Spectrometry; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C092281:PSI protein, Drosophila; D016601:RNA-Binding Proteins; C498930:tlk protein, Drosophila; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases