title: Rapid degradation of poly(ADP-ribose) after injection into the mouse bloodstream.
abstract: Extensive DNA damage leads to the activation of poly(ADP-ribose) polymerase and subsequently to the formation of poly(ADP-ribose). When the damage is severe or leads to cell death, poly(ADP-ribose) may leak into the blood circulation. The metabolism of poly(ADP-ribose) in the bloodstream is not well understood. Thus, in the present study, the metabolism of P-labeled poly(ADP-ribose) was followed in mice after injection of this labeled compound into the tail vein. The results showed that 5 min after injection more than half of the radioactivity was concentrated in acid-soluble fractions, namely in low molecular weight compounds in the blood, liver, and kidneys. Most of this radioactivity was in the form of inorganic phosphate, detected 5 min post-injection in the blood, kidneys, and urine. By contrast, the metabolites ADP-ribose and phosphoribosyl-AMP were not detected in any of the tissues nor in blood or urine. Taken together, these findings suggest that once poly(ADP-ribose) enters the bloodstream it is rapidly degraded, thereby preventing its accumulation in the blood.
authors: Division of Genome Stability Research, National Cancer Center Research Institute, 5–1–1 Tsukiji, Chuo-ku, Tokyo 104–0045, Japan.
authors: Yasuhisa
authors: Y
authors: Okajima
authors: Tomoko
authors: T
authors: Yoshida
authors: Hiroaki
authors: H
authors: Fujimori
authors: Junhui
authors: J
authors: Wang
authors: Hiromi
authors: H
authors: Harada
authors: Ylenia
authors: Y
authors: Suzuki
authors: Hisanori
authors: H
authors: Suzuki
authors: Mitsuko
authors: M
authors: Masutani
mesh_terms: D000818:Animals; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011064:Poly Adenosine Diphosphate Ribose / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D011064:Poly Adenosine Diphosphate Ribose