title: The role of interactions between bacterial chaperone, aspartate aminotransferase, and viral protein during virus infection in high temperature environment: the interactions between bacterium and virus proteins.
abstract: BACKGROUND
  The life cycle of a bacteriophage has tightly programmed steps to help virus infect its host through the interactions between the bacteriophage and its host proteins. However, bacteriophage-host protein interactions in high temperature environment remain poorly understood. To address this issue, the protein interaction between the thermophilic bacteriophage GVE2 and its host thermophilic Geobacillus sp. E263 from a deep-sea hydrothermal vent was characterized.
  
  
  RESULTS
  This investigation showed that the host's aspartate aminotransferase (AST), chaperone GroEL, and viral capsid protein VP371 formed a linearly interacted complex. The results indicated that the VP371-GroEL-AST complex were up-regulated and co-localized in the GVE2 infection of Geobacillus sp. E263.
  
  
  CONCLUSIONS
  As reported, the VP371 is a capsid protein of GVE2 and the host AST is essential for the GVE2 infection. Therefore, our study revealed that the phage could use the anti-stress system of its host to protect the virus reproduction in a high-temperature environment for the first time.
authors: Zhejiang University, Hangzhou, The People's Republic of China.
authors: Yanjiang
authors: Y
authors: Chen
authors: Dahai
authors: D
authors: Wei
authors: Yiqian
authors: Y
authors: Wang
authors: Xiaobo
authors: X
authors: Zhang
mesh_terms: D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001435:Bacteriophages / Q000254:growth & development*; D036022:Capsid Proteins / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D056506:Geobacillus / Q000821:virology*; D006790:Host-Parasite Interactions; D060086:Hydrothermal Vents / Q000382:microbiology / Q000821:virology; D025941:Protein Interaction Mapping; D012623:Seawater / Q000382:microbiology / Q000821:virology; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D036022:Capsid Proteins; D018834:Chaperonin 60; D001219:Aspartate Aminotransferases