title: Simultaneous quantification of flavonoids in blood plasma by a high-performance liquid chromatography method after oral administration of Blumea balsamifera leaf extracts in rats.
abstract: The leaves of Blumea balsamifera are used as a folk medicine in kidney stone diseases in South-East Asia. Phytochemical investigation revealed leaves contained a number of flavonoids. In view of these, the present work was aimed to quantify and preliminary pharmacokinetic investigation of five flavonoids viz. dihydroquercetin-7,4¢-dimethyl ether (I), dihydroquercetin-4¢-methyl ether (II), 5,7,3¢,5¢-tetrahydroxyflavanone (III), blumeatin (IV) and quercetin (V) in rat plasma following oral administration (0.5g/Kg) of B. balsamifera leaf extract in rats. Quantification was achieved by using a validated, reproducible high-performance liquid chromatographic method. The mean recoveries of I, II, III, IV and V were 90.6, 93.4, 93.5, 91.2 and 90.3% respectively. The limit of quantification was 25 ng/mL for I and IV, 10 ng/mL for II and III and 100 ng/mL for V respectively. The within day and day-to-day precision for all the compounds were < 10%. The validated HPLC method herein was applied for pharmacokinetic studies and the main pharmacokinetic parameters were: t1/2 (hr) 5.8, 4.3, 2.9, 5.7 and 7.3, Cmax (ng/mL) 594.9, 1542.9 1659.9, 208.9 and 3040.4; Tmax (hr) 4.7, 1.0, 1.0, 3.5 and 2.3; AUC0-oo (ng hr/mL) 5040, 5893, 9260, 1064 and 27233 for I, II, III, IV and V respectively. The developed method was suitable for pharmacokinetic studies and this preliminary study also revealed significant absorption after oral dosing in rats.
authors: Pharmaceutical & Medicinal Chemistry Department, Dubai Pharmacy College, Dubai, UAE.
authors: Fazilatun
authors: F
authors: Nessa
authors: Zhari
authors: Z
authors: Ismail
authors: Nornisah
authors: N
authors: Mohamed
authors: Sundram
authors: S
authors: Karupiah
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000008:administration & dosage* / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D006207:Half-Life; D007408:Intestinal Absorption; D057230:Limit of Detection; D008297:Male; D008657:Metabolic Clearance Rate; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D018515:Plant Leaves; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D012997:Solvents; D000432:Methanol