title: Class IIa histone deacetylases and myocyte enhancer factor 2 proteins regulate the mesenchymal-to-epithelial transition of somatic cell reprogramming.
abstract: Class IIa histone deacetylases (HDACs) and myocyte enhancer factor 2 (MEF2) proteins compose a signaling module that orchestrates lineage specification during embryogenesis. We show here that this module also regulates the generation of mouse induced pluripotent stem cells by defined transcription factors. Class IIa HDACs and MEF2 proteins rise steadily during fibroblast reprogramming to induced pluripotent stem cells. MEF2 proteins tend to block the process by inducing the expression of Tgfβ cytokines, which impairs the necessary phase of mesenchymal-to-epithelial transition (MET). Conversely, class IIa HDACs endeavor to suppress the activity of MEF2 proteins, thus enhancing the MET and colony formation efficiency. Our work highlights an unexpected role for a developmental axis in somatic cell reprogramming and provides new insight into how the MET is regulated in this context.
authors: Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou 510530, China and.
authors: Qiang
authors: Q
authors: Zhuang
authors: Xiaobing
authors: X
authors: Qing
authors: Yue
authors: Y
authors: Ying
authors: Haitao
authors: H
authors: Wu
authors: Christina
authors: C
authors: Benda
authors: Jiao
authors: J
authors: Lin
authors: Zhijian
authors: Z
authors: Huang
authors: Longqi
authors: L
authors: Liu
authors: Yan
authors: Y
authors: Xu
authors: Xichen
authors: X
authors: Bao
authors: Baoming
authors: B
authors: Qin
authors: Duanqing
authors: D
authors: Pei
authors: Miguel A
authors: MA
authors: Esteban
mesh_terms: D000818:Animals; D054337:Cell Dedifferentiation / Q000187:drug effects / Q000502:physiology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects / Q000502:physiology*; D057809:HEK293 Cells; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D018006:Myogenic Regulatory Factors / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics
chemical_list: D056572:Histone Deacetylase Inhibitors; D018006:Myogenic Regulatory Factors; D016212:Transforming Growth Factor beta; D006655:Histone Deacetylases