title: Cholesterol suppresses adipocytic differentiation of mouse adipose-derived stromal cells via PPARγ2 signaling.
abstract: The effects of cholesterol on cell proliferation and adipocytic differentiation have been evaluated for the first time in mouse adipose-derived stromal cells (mASCs). Cholesterol loading by using cholesterol:methyl- β-cyclodextrin (Chol:MβCD) complexes promoted cellular levels of free cholesterol (FC) and cholesteryl ester (CE), induced high cell proliferation of mASCs dose-dependently. Compared with control cells, cholesterol-treated mASCs showed an impaired differentiation process in both dose- and time-dependent manners, based on reduced oil red O-stained lipid droplets, SREBP-1, PPARγ2 and aP2 expression levels. The involvement of SREBP-1-mediated PPARγ2 in the effects of cholesterol on mASC adipogenesis was elucidated. These results point to cholesterol as a modulator of adipogenesis, which separate cholesterol itself from other components of modified lipoproteins.
authors: College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China. hfli1228@163.com
authors: Haifang
authors: H
authors: Li
authors: Han
authors: H
authors: Li
authors: Hengjun
authors: H
authors: Guo
authors: Feihan
authors: F
authors: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology*; D002788:Cholesterol Esters / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D012995:Solubility; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000378:metabolism
chemical_list: D002788:Cholesterol Esters; D047495:PPAR gamma; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D014867:Water; D002784:Cholesterol