title: Repairing cartilage defects with bone marrow mesenchymal stem cells induced by CDMP and TGF-β1.
abstract: The aim of this study was to explore the ability for chondrogenic differentiation of bone marrow mesenchymal stems cells (BMSCs) induced by either cartilage-derived morphogenetic protein 1 (CDMP-1) alone or in the presence of transforming growth factor-β1 (TGF-β1) in vivo and in vitro. BMSCs and poly-lactic acid/glycolic acid copolymer (PLGA) scaffold were analyzed for chondrogenic capacity induced by CDMP-1 and TGF-β1 in vivo and in vitro. Chondrogenic differentiation of BMSCs into chondrocytes using a high density pellet culture system was tested, whether they could be maintained in 3-D PLGA scaffold instead of pellet culture remains to be explored. Under the culture of high-density cell suspension and PLGA frame, BMSCs were observed the ability to repair cartilage defects by either CDMP-1 alone or in the presence of TGF-β1 in vitro. Then the cell-scaffold complex was implanted into animals for 4 and 8 weeks for in vivo test. The content of collagen type II and proteoglycan appeared to increase over time in the constructs of the induced groups (CDMP in the presence of TGF-β1), CDMP group and TGF group. However, the construct of the control group did not express them during the whole culture time. At 4 and 8 weeks, the collagen type II expression of the induced group was higher than the sum of TGF group and CDMP group by SSPS17.0 analysis. BMSCs and PLGA complex induced by CDMP-1 and TGF- β1 can repair cartilage defects more effectively than that induced by CDMP-1 or TGF-β1 only.
authors: Department of Surgery, The First Affiliated Hospital of Liaoning Medical College, Jinzhou City, 121001, Liaoning Province, China.
authors: Gang
authors: G
authors: Wu
authors: Ying
authors: Y
authors: Cui
authors: Linxiang
authors: L
authors: Ma
authors: Xinyu
authors: X
authors: Pan
authors: Xuefeng
authors: X
authors: Wang
authors: Ben
authors: B
authors: Zhang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002356:Cartilage / Q000293:injuries*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D020219:Chondrogenesis; D024043:Collagen Type II / Q000096:biosynthesis; D055428:Growth Differentiation Factor 5 / Q000494:pharmacology*; D048091:Guided Tissue Regeneration / Q000379:methods*; D019344:Lactic Acid / Q000494:pharmacology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D011100:Polyglycolic Acid / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: D024043:Collagen Type II; D055428:Growth Differentiation Factor 5; D053773:Transforming Growth Factor beta1; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid