title: Synthesis, characterization and bioevaluation of technetium-99m labeled N-(2-Hydroxybenzyl)-2-amino-2-deoxy-D-glucose as a tumor imaging agent.
abstract: N-(2-Hydroxybenzyl)-2-amino-2-deoxy-D-glucose (NHADG) was synthesized by conjugation of salicylaldehyde to glucosamine. The obtained compound was well characterized via different analytical techniques. Labeling of the synthesized compound with technetium-99m ((99m)Tc) in pertechnetate form ((99m)Tc O4-) was carried out via chelation reaction in the presence of stannous chloride dihydrate. Maximum radiochemical yield of (99m)Tc-NHADG complex (99%) was obtained by using 1 mg NHADG, 200 μg SnCl2.2H2O, at pH 9.5 and reaction time of 15 min. The radiochemical purity of the (99m)Tc-NHADG complex was measured by instant thin layer chromatography (ITLC) and paper chromatography (PC), without any notable decomposition at room temperature over a period of 4h. The biological evaluation results show that the (99m)Tc labeled NHADG conjugate is able to specifically target mammary carcinoma in mice models, thus highlighting its potential as an effective (99m)Tc labeled glucose-derived agent for tumor imaging.
authors: Institute of Nuclear Medicine & Oncology, Lahore, Pakistan.
authors: Qaisar
authors: Q
authors: Nadeem
authors: Irfanullah
authors: I
authors: Khan
authors: Muhammad
authors: M
authors: Javed
authors: Zaid
authors: Z
authors: Mahmood
authors: Ume-Kalsoom
authors: UK
authors: Dar
authors: Muhammad
authors: M
authors: Ali
authors: Syed Waqar
authors: SW
authors: Hyder
authors: Sohail
authors: S
authors: Murad
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D007553:Isotope Labeling; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D013667:Technetium; D013696:Temperature; D017971:Tin Compounds / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: C495579:N-(2'-hydroxybenzyl)-2-amino-2-deoxyglucose; D019275:Radiopharmaceuticals; D017971:Tin Compounds; C023599:stannous chloride; D013667:Technetium; D005944:Glucosamine