title: Stability of puroindoline peptides and effects on wheat rust.
abstract: Peptides modelled on the tryptophan rich domain of puroindolines and the related grain softness protein-1 have a broad range of antibacterial and antifungal activities. With the aims of further investigating the activities of these antimicrobial peptides we studied their activity against wheat rust diseases and environmental stability. PINA-based peptides were found to have high pH and thermal stability in addition to being stable over long periods at room temperature. These properties could make them excellent candidates as preservatives in food. PuroA, Pina-R39G and PuroB peptides adversely affected the morphology of the stripe rust spores (Puccinia striiformis f. sp. tritici), while PuroA and PuroB showed moderate inhibition of their germination. Additionally, GSP-5D reduced the germination of leaf rust spores (P. triticina). PuroA and PuroB sprayed onto stripe rust infected plants effected a moderate reduction in the number of stripe rust uredinia on wheat seedlings, as did PuroB sprayed onto the seedlings and allowed to coat the leaves for 5 day prior to spore infection. The results suggest that the presence of the PIN-based peptides may lower frequency of initial infection foci.
authors: Faculty of Life and Social Sciences, Environment and Biotechnology Centre, Swinburne University of Technology, PO Box 218, Melbourne, VIC 3122, Australia.
authors: Rebecca L
authors: RL
authors: Alfred
authors: Enzo A
authors: EA
authors: Palombo
authors: Joseph F
authors: JF
authors: Panozzo
authors: Harbans
authors: H
authors: Bariana
authors: Mrinal
authors: M
authors: Bhave
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001487:Basidiomycota / Q000187:drug effects* / Q000254:growth & development; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D010940:Plant Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development; D014908:Triticum / Q000737:chemistry / Q000382:microbiology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D010940:Plant Proteins; C082942:puroindoline protein, Triticum aestivum