title: Kinetics of IL-23 and IL-12 secretion in response to Toxoplasma gondii antigens from THP-1 monocytic cells.
abstract: IL-23 and IL-12 are structurally similar and critical for the generation of efficient cellular immune responses. Toxoplasma gondii induces a strong cell-mediated immune response. However, little is known about IL-23 secretion profiles in T. gondii-infected immune cells in connection with IL-12. We compared the patterns of IL-23 and IL-12 production by THP-1 human monocytic cells in response to stimulation with live or heat-killed T. gondii tachyzoites, or with equivalent quantities of either T. gondii excretory/secretory proteins (ESP) or soluble tachyzoite antigen (STAg). IL-23 and IL-12 were significantly increased from 6 hr after stimulation with T. gondii antigens, and their secretions were increased with parasite dose-dependent manner. IL-23 concentrations were significantly higher than those of IL-12 at the same multiplicity of infection. IL-23 secretion induced by live parasites was significantly higher than that by heat-killed parasites, ESP, or STAg, whereas IL-12 secretion by live parasite was similar to those of ESP or STAg. However, the lowest levels of both cytokines were at stimulation with heat-killed parasites. These data indicate that IL-23 secretion patterns by stimulation with various kinds of T. gondii antigens at THP-1 monocytic cells are similar to those of IL-12, even though the levels of IL-23 induction were significantly higher than those of IL-12. The detailed kinetics induced by each T. gondii antigen were different from each other.
authors: Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors: Juan-Hua
authors: JH
authors: Quan
authors: Wei
authors: W
authors: Zhou
authors: Guang-Ho
authors: GH
authors: Cha
authors: In-Wook
authors: IW
authors: Choi
authors: Dae-Whan
authors: DW
authors: Shin
authors: Young-Ha
authors: YH
authors: Lee
mesh_terms: D000953:Antigens, Protozoan / Q000276:immunology*; D002460:Cell Line; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism*; D053759:Interleukin-23 / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology* / Q000469:parasitology*; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D053759:Interleukin-23; D018664:Interleukin-12
keywords: IL-12; IL-23; THP-1 monocytic cell; Toxoplasma gondii