title: Voltage-gated membrane currents in neurons involved in odor information processing in snail procerebrum.
abstract: The procerebrum (PC) of the snail brain is a critical region for odor discrimination and odor learning. The morphological organization and physiological function of the PC has been intensively investigated in several gastropod species; however, the presence and distribution of ion channels in bursting and non-bursting cells has not yet been described. Therefore, the aim of our study was to identify the different ion channels present in PC neurons. Based on whole cell patch-clamp and immunohistochemical experiments, we show that Na(+)-, Ca(2+)-, and K(+)-dependent voltage-gated channels are differentially localized and expressed in the cells of the PC. Different Na-channel subtypes are present in large (10-15 μm) and small (5-8 μm) diameter neurons, which are thought to correspond to the bursting and non-bursting cells, respectively. Here, we show that the bursting neurons possess fast sodium current (I NaT) and NaV1.9-like channels and the non-bursting neurons possess slow sodium current (I NaT) and NaV1.8-like channels in addition to the L-type Ca(-), KV4.3 (A-type K-channel) and KV2.1 channels. We suggest that the bursting and/or non-bursting character of the PC neurons is at least partly determined by the battery of ion-channels present and their cellular and subcellular compartmentalization.
authors: Department of Experimental Zoology, Balaton Limnological Institute, Centre for Ecological Research, Hungarian Academy of Sciences, Klebelsberg Kuno Str. 3, 8237, Tihany, Hungary.
authors: Z
authors: Z
authors: Pirger
authors: I
authors: I
authors: Battonyai
authors: N
authors: N
authors: Krajcs
authors: K
authors: K
authors: Elekes
authors: T
authors: T
authors: Kiss
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D055592:Biophysical Phenomena / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000166:cytology*; D019256:Cadmium Chloride / Q000494:pharmacology; D004558:Electric Stimulation; D006372:Helix, Snails / Q000166:cytology / Q000502:physiology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D009812:Odorants; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000494:pharmacology; D011984:Sensory Receptor Cells / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D019789:Tetraethylammonium / Q000494:pharmacology
chemical_list: D007473:Ion Channels; D026902:Potassium Channel Blockers; D019789:Tetraethylammonium; D015761:4-Aminopyridine; D019256:Cadmium Chloride