title: [Histone acetylation modulates influenza virus replicative intermediate dsRNA-induced interleukin-6 expression in A549 cells].
abstract: OBJECTIVE
  To investigate the role of histone acetylation in regulating influenza virus replicative intermediate double-stranded RNA (dsRNA)-induced interleukin-6 (IL-6) expression in A549 cells.
  
  
  METHODS
  A549 cells were treated with influenza virus replicative intermediate dsRNA, histone deacetylase (HDAC) inhibitor trichostatin A (TSA), or HADC small interfering RNA (siRNA). The changes in the cellular IL-6 promoter activities were detected by dual-luciferase assay, and IL-6 mRNA and protein expressions in the cells were determined using real-time RT-PCR and ELISA, respectively.
  
  
  RESULTS
  Influenza virus replicative intermediate dsRNA obviously up-regulated IL-6 expression in the cells. HDAC inhibitor TSA significantly enhanced the activity of IL-6 promoter and increased IL-6 mRNA expression in A549 cells, and HDAC3 may play an important role in this process. HDAC inhibitor TSA and DNMT inhibitor DAC showed no synergic effect in regulating IL-6 expression.
  
  
  CONCLUSIONS
  Influenza virus replicative intermediate dsRNA-induced IL-6 expression in A549 cells is regulated by histone acetylation.
authors: Department of Life Science, Bengbu Medical College, Bengbu, China. bktang.bbmc@gmail.com
authors: Bikui
authors: B
authors: Tang
authors: Shouwei
authors: S
authors: Wu
authors: Mingjie
authors: M
authors: Hu
authors: Xiaofen
authors: X
authors: Liu
mesh_terms: D000107:Acetylation; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012330:RNA, Double-Stranded; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; C508600:IL6 protein, human; D015850:Interleukin-6; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral