title: Laccase production by Coriolopsis caperata RCK2011: optimization under solid state fermentation by Taguchi DOE methodology.
abstract: Laccase production by Coriolopsis caperata RCK2011 under solid state fermentation was optimized following Taguchi design of experiment. An orthogonal array layout of L18 (2(1) × 3(7)) was constructed using Qualitek-4 software with eight most influensive factors on laccase production. At individual level pH contributed higher influence, whereas, corn steep liquor (CSL) accounted for more than 50% of the severity index with biotin and KH2PO4 at the interactive level. The optimum conditions derived were; temperature 30°C, pH 5.0, wheat bran 5.0 g, inoculum size 0.5 ml (fungal cell mass = 0.015 g dry wt.), biotin 0.5% w/v, KH2PO4 0.013% w/v, CSL 0.1% v/v and 0.5 mM xylidine as an inducer. The validation experiments using optimized conditions confirmed an improvement in enzyme production by 58.01%. The laccase production to the level of 1623.55 Ugds(-1) indicates that the fungus C. caperata RCK2011 has the commercial potential for laccase.
authors: Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi-110021, India.
authors: Preeti
authors: P
authors: Nandal
authors: Sreenivas Rao
authors: SR
authors: Ravella
authors: Ramesh Chander
authors: RC
authors: Kuhad
mesh_terms: D000704:Analysis of Variance; D019149:Bioreactors; D005285:Fermentation; D042845:Laccase / Q000096:biosynthesis*; D013149:Spiroplasma / Q000254:growth & development / Q000378:metabolism*
chemical_list: D042845:Laccase