title: Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1.
abstract: RNA interference (RNAi) is a biological phenomenon that silences the expression of genes of interest. Passive double-stranded RNA (dsRNA) uptake has been uniquely observed in Caenorhabditis elegans due to the expression of systemic RNAi defective-1 (SID-1). We report that ectopic expression of CeSID-1 endows the Sf9 cells with a capacity for soaking RNAi. Soaking the Sf9-SID1 with dsRNA corresponding to either exogenous or endogenous target genes induced a significant decrease in the amount of mRNA or protein. These results enabled us to modify the target proteins of baculovirus expression vector system in both quantities and posttranslational modifications. The current low-cost and high-efficiency RNAi system is useful for high-throughput gene function analysis and mass production of recombinant protein.
authors: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki Higashi-ku, Fukuoka 812-8581, Japan.
authors: Jian
authors: J
authors: Xu
authors: Yudai
authors: Y
authors: Nagata
authors: Hiroaki
authors: H
authors: Mon
authors: Zhiqing
authors: Z
authors: Li
authors: Li
authors: L
authors: Zhu
authors: Kazuhiro
authors: K
authors: Iiyama
authors: Takahiro
authors: T
authors: Kusakabe
authors: Jae Man
authors: JM
authors: Lee
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000254:growth & development*; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000235:genetics*; D020868:Gene Silencing; D005822:Genetic Vectors; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D061987:Sf9 Cells; D018411:Spodoptera
chemical_list: D029742:Caenorhabditis elegans Proteins; D008565:Membrane Proteins; C452203:SID-1 protein, C elegans