title: Acute pancreatitis is associated with Ser608Leu iNOS polymorphism.
abstract: Acute pancreatitis is an initially localized           inflammation of the pancreatic gland. The precise           mechanisms by which aetiological factors induce acute           pancreatitis are not yet known, but when initiated,           common inflammatory pathways seem to be involved,           with cytokines being their components of major           importance. The inducible nitric oxide synthase gene           (iNOS) encodes an enzyme involved in the pathway           of reactive oxygen species and induced in response to           infection, cytokines. iNOS is capable of generating           large quantities of nitric oxide produced during inflammation.           The objective of this study was to investigate           the association between acute pancreatitis risk           and iNOS polymorphisms. The studied single-nucleotide           polymorphisms (SNPs) were Ser608Leu, resulting           in an amino acid substitution, and 1173C/T           and 954G/C, both in the gene promoter region that is           linked to increased enzyme expression, leading to           higher NO production. The genotypes for the three           SNPs were determined in 93 patients with acute pancreatitis           and 60 controls without pancreatitis or cancer           that were matched for age and gender. Data analysis           was done by conditional logistic regression. It           was found that the Ser608Leu polymorphism was           more frequent among cases with acute pancreatitis           compared to controls (OR = 2.88; 95% CI: 1.49-5.57;           P = 0.002), although no individually statistically significant           associations for the other SNPs studied were detected. We suggest that iNOS Ser608Leu can be           used as a marker to define the risk of acute pancreatitis.
authors: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Faculty of Medicine, Istanbul University, Istanbul, Turkey. gulozhan@istanbul.edu.tr
authors: G
authors: G
authors: Özhan
authors: F M
authors: FM
authors: Sari
authors: M
authors: M
authors: Vefai
authors: H T
authors: HT
authors: Yanar
authors: B
authors: B
authors: Alpertunga
mesh_terms: D000208:Acute Disease; D000368:Aged; D019943:Amino Acid Substitution / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D010195:Pancreatitis / Q000201:enzymology* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II