title: Zizyphus oenophlia: a potent substrate for lactic acid production.
abstract: The objective of the present investigation deals with optimization of L-lactic acid production from Zizyphus oenophlia, a potential low cost substrate. Central composite design (CCD) based on response surface methodology (RSM) was employed as a statistical tool to investigate the effect of substrate: media ratio, temperature, pH and inoculum volume on lactic acid production. Under optimum condition, the experimental yield was 95.09%, which matched well with the predictive yield of 95.45%. HPLC and circular dichroism (CD) analysis of the fermented product was carried out to confirm the presence of lactic acid in its L (+) seriospecificity respectively. For application of lactic acid in biotechnological sector polymerization studies were carried out. The PLA upon recovery through direct polycondensation reaction resulted in the yield of 85%.
authors: Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, India.
authors: Moumita
authors: M
authors: Bishai
authors: Swarnalok
authors: S
authors: De
authors: Basudam
authors: B
authors: Adhikari
authors: Rintu
authors: R
authors: Banerjee
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis* / Q000737:chemistry; D007778:Lactobacillus / Q000378:metabolism; D008970:Molecular Weight; D011091:Polyesters; D011108:Polymers; D013237:Stereoisomerism; D014783:Viscosity; D031957:Ziziphus / Q000378:metabolism*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)