title: Age-dependent reduction of ghrelin- and motilin-induced contractile activity in the chicken gastrointestinal tract.
abstract: Ghrelin is an endogenous ligand for growth hormone secretagogue-receptor 1a (GHS-R1a) and stimulates gastrointestinal (GI) motility in the chicken. Since ghrelin stimulates GH release, which regulates growth, it might be interesting to compare ghrelin-induced responses in GI tract of different-aged chickens. Motilin is a ghrelin-related gut peptide that induces strong contraction in the small intestine. Aim of this study was to clarify age-dependent changes in ghrelin- and motilin-induced contractions of the chicken GI tract and expression of their receptor mRNAs. Chicken ghrelin caused contraction of the crop and proventriculus. Ghrelin-induced contraction in the proventriculus decreased gradually up to 100 days after hatching, but the responses to ghrelin in the crop were the same during the growth period. GHS-R1a mRNA expression in the crop tended to increase, but that in the proventriculus decreased depending on the age. Chicken motilin caused contraction of the chicken GI tract. Atropine decreased the responses to motilin in the proventriculus but not in the ileum. Motilin-induced contraction in the proventriculus but not that in the ileum decreased depending on post-hatching days. On the other hand, motilin receptor mRNA expression in every region of the GI tract decreased with age, but the decrease was more marked in the proventriculus than in the ileum. In conclusion, ghrelin- and motilin-induced GI contractions selectively decreased in the chicken proventriculus depending on post-hatching days, probably due to the age-related decrease in respective receptors expression. The results suggest an age-related contribution of ghrelin and motilin to the regulation of chicken GI motility.
authors: Department of Veterinary Science, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. tko-kita@rakuno.ac.jp
authors: Takio
authors: T
authors: Kitazawa
authors: Akiko
authors: A
authors: Yoshida
authors: Takuya
authors: T
authors: Tamano
authors: Hiroki
authors: H
authors: Teraoka
authors: Hiroyuki
authors: H
authors: Kaiya
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002645:Chickens; D041981:Gastrointestinal Tract / Q000187:drug effects*; D054439:Ghrelin / Q000494:pharmacology*; D009037:Motilin / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects*; D010084:Oxidation-Reduction
chemical_list: D054439:Ghrelin; D009037:Motilin