title: Cell separation: Terminology and practical considerations.
abstract: Cell separation is a powerful tool in biological research. Increasing usage, particularly within the tissue engineering and regenerative medicine communities, means that researchers from a diverse range of backgrounds are utilising cell separation technologies. This review aims to offer potential solutions to cell sorting problems and to clarify common ambiguities in terminology and experimental design. The frequently used cell separation terms of 'purity', 'recovery' and 'viability' are discussed, and attempts are made to reach a consensus view of their sometimes ambiguous meanings. The importance of appropriate experimental design is considered, with aspects such as marker expression, tissue isolation and original cell population analysis discussed. Finally, specific technical issues such as cell clustering, dead cell removal and non-specific antibody binding are considered and potential solutions offered. The solutions offered may provide a starting point to improve the quality of cell separations achieved by both the novice and experienced researcher alike.
authors: Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UK.
authors: Matthew J
authors: MJ
authors: Tomlinson
authors: Sophie
authors: S
authors: Tomlinson
authors: Xuebin B
authors: XB
authors: Yang
authors: Jennifer
authors: J
authors: Kirkham
keywords: cell clusters; cell separation; cell sorting; dead cells; terminology; tissue preparation