title: Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells.
abstract: The uptake of calcium phosphate nanoparticles (diameter 120nm) with different charge by HeLa cells was studied by flow cytometry. The amount of uptaken nanoparticles increased with increasing concentration of nanoparticles in the cell culture medium. Several inhibitors of endocytosis and macropinocytosis were applied to elucidate the uptake mechanism of nanoparticles into HeLa cells: wortmannin, LY294002, nocodazole, chlorpromazine and nystatin. Wortmannin and LY294002 strongly reduced the uptake of anionic nanoparticles, which indicates macropinocytosis as uptake mechanism. For cationic nanoparticles, the uptake was reduced to a lesser extent, indicating a different uptake mechanism. The localization of nanoparticles inside the cells was investigated by conjugating them with the pH-sensitive dye SNARF-1. The nanoparticles were localized in lysosomes after 3h of incubation.
authors: Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaets str. 5-7, D-45117 Essen, Germany.
authors: Viktoriya
authors: V
authors: Sokolova
authors: Diana
authors: D
authors: Kozlova
authors: Torben
authors: T
authors: Knuschke
authors: Jan
authors: J
authors: Buer
authors: Astrid M
authors: AM
authors: Westendorf
authors: Matthias
authors: M
authors: Epple
mesh_terms: D000838:Anions; D002130:Calcium Phosphates / Q000737:chemistry*; D002412:Cations; D002462:Cell Membrane / Q000737:chemistry*; D004705:Endocytosis; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D022161:Transport Vesicles / Q000737:chemistry*
chemical_list: D000838:Anions; D002130:Calcium Phosphates; D002412:Cations; C020243:calcium phosphate