title: Nox2 modification of LDL is essential for optimal apolipoprotein B-mediated control of agr type III Staphylococcus aureus quorum-sensing.
abstract: Staphylococcus aureus contains an autoinducing quorum-sensing system encoded within the agr operon that coordinates expression of virulence genes required for invasive infection. Allelic variation within agr has generated four agr specific groups, agr I-IV, each of which secretes a distinct autoinducing peptide pheromone (AIP1-4) that drives agr signaling. Because agr signaling mediates a phenotypic change in this pathogen from an adherent colonizing phenotype to one associated with considerable tissue injury and invasiveness, we postulated that a significant contribution to host defense against tissue damaging and invasive infections could be provided by innate immune mechanisms that antagonize agr signaling. We determined whether two host defense factors that inhibit AIP1-induced agrI signaling, Nox2 and apolipoprotein B (apoB), also contribute to innate control of AIP3-induced agrIII signaling. We hypothesized that apoB and Nox2 would function differently against AIP3, which differs from AIP1 in amino acid sequence and length. Here we show that unlike AIP1, AIP3 is resistant to direct oxidant inactivation by Nox2 characteristic ROS. Rather, the contribution of Nox2 to defense against agrIII signaling is through oxidation of LDL. ApoB in the context of oxLDL, and not LDL, provides optimal host defense against S. aureus agrIII infection by binding the secreted signaling peptide, AIP3, and preventing expression of the agr-driven virulence factors which mediate invasive infection. ApoB within the context of oxLDL also binds AIP 1-4 and oxLDL antagonizes agr signaling by all four agr alleles. Our results suggest that Nox2-mediated oxidation of LDL facilitates a conformational change in apoB to one sufficient for binding and sequestration of all four AIPs, demonstrating the interdependence of apoB and Nox2 in host defense against agr signaling. These data reveal a novel role for oxLDL in host defense against S. aureus quorum-sensing signaling.
authors: Department of Pharmaceutical Sciences, University of New Mexico College of Pharmacy, Albuquerque, New Mexico, USA. phall@salud.unm.edu
authors: Pamela R
authors: PR
authors: Hall
authors: Bradley O
authors: BO
authors: Elmore
authors: Cynthia H
authors: CH
authors: Spang
authors: Susan M
authors: SM
authors: Alexander
authors: Brett C
authors: BC
authors: Manifold-Wheeler
authors: Moriah J
authors: MJ
authors: Castleman
authors: Seth M
authors: SM
authors: Daly
authors: M Michal
authors: MM
authors: Peterson
authors: Erin K
authors: EK
authors: Sully
authors: Jon K
authors: JK
authors: Femling
authors: Michael
authors: M
authors: Otto
authors: Alexander R
authors: AR
authors: Horswill
authors: Graham S
authors: GS
authors: Timmins
authors: Hattie D
authors: HD
authors: Gresham
mesh_terms: D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D007113:Immunity, Innate; D007118:Immunoassay; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000502:physiology*; D053038:Quorum Sensing / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011973:Receptors, LDL / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D013211:Staphylococcus aureus / Q000502:physiology*; D020349:Surface Plasmon Resonance; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: C095698:Agr protein, Staphylococcus aureus; D001055:Apolipoproteins B; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011973:Receptors, LDL; D015534:Trans-Activators; C488842:Cybb protein, mouse; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases