title: Dynamics of focal adhesions and reorganization of F-actin in VEGF-stimulated NSCs under varying differentiation states.
abstract: Precise migration of neural stem/progenitor cells (NSCs) is crucially important for neurogenesis and repair in the nervous system. However, the detailed mechanisms are not clear. Our previous results showed that NSCs in varying differentiation states possess different migratory ability to vascular endothelial growth factor (VEGF). In this study, we demonstrate the different dynamics of focal adhesions (FAs) and reorganization of F-actin in NSCs during spreading and migration stimulated by VEGF. We found that the migrating NSCs of 0.5 and 1 day differentiation possess more FAs at leading edge than cells of other states. Moreover, the phosphorylation of focal adhesion kinase (FAK) and paxillin in NSCs correlates closely with their differentiation states. VEGF promotes FA formation with broad lamellipodium generation at the leading edge in chemotaxing cells of 0, 0.5, and 1 day differentiation, but not in cells of 3 days differentiation. Furthermore, cells of 1 day differentiation show a maximal asymmetry of FAs between lamella and cell rear, orchestrating cell polarization and directional migration. Time-lapse video analysis shows that the disassembly of FAs and the cell tail detachment in NSCs of 1 day differentiation are more rapid, along with the concurrent enlarged size of FAs at the leading edge, leading to the most effective chemotactic response to VEGF. Collectively, these results indicate that the dynamics of FAs and reorganization of F-actin in NSCs that undergo directional migration correlate closely with their differentiation states, contributing to the different chemotactic responses of these cells to VEGF.
authors: Department of Cell Biology, Medical College of Soochow University, Jiangsu Key Laboratory of Stem Cell Research, Ren Ai Road 199, Suzhou Industrial Park, Suzhou 215123, China.
authors: Jingya
authors: J
authors: Lyu
authors: Ya'nan
authors: Y
authors: Hu
authors: Xiaojing
authors: X
authors: Xu
authors: Huanxiang
authors: H
authors: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D002633:Chemotaxis / Q000187:drug effects*; D022001:Focal Adhesions / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D000199:Actins; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat