title: C-C motif chemokine receptor 9 positive macrophages activate hepatic stellate cells and promote liver fibrosis in mice.
abstract: Chemokine receptors mediate migration of immune cells into the liver, thereby promoting liver inflammation. C-C motif chemokine receptor (CCR) 9(+) macrophages are crucial in the pathogenesis of acute liver inflammation, but the role and underlying mechanisms of this macrophage subset in chronic liver injury and subsequent liver fibrosis are not fully understood. We confirmed that tumor necrosis factor alpha (TNF-α)-producing CCR9(+) macrophages accumulated during the initiation of carbon tetrachloride (CCl4 )-induced liver injury, and CCR9 deficiency attenuated the degree of liver damage. Accumulation of CCR9(+) macrophages persisted prominently during the process of liver fibrosis induced by repetitive CCl4 or thioacetamide (TAA)/leptin administration. Increased CCR9 expression was also found on activated hepatic stellate cells (HSCs). Importantly, experimental liver fibrosis was significantly ameliorated in CCR9(-/-) mice compared with wild-type (WT) mice, assessed by α-smooth muscle actin (α-SMA) immunostain, Sirius red staining, and messenger RNA (mRNA) expression levels of α-SMA, collagen 1α1, transforming growth factor (TGF)-β1, and tissue inhibitor of metalloproteinase (TIMP)-1. Accumulated CD11b(+) macrophages in CCl4 -treated WT mice showed marked increases in TNF, NO synthase-2, and TGF-β1 mRNA expression compared with CCR9(-/-) mice, implying proinflammatory and profibrogenic properties. Hepatic CD11b(+) macrophages from CCl4 -treated WT mice (i.e., CCR9(+) macrophages), but not CD8(+) T lymphocytes or non-CD11b(+) cells, significantly activated HSCs in vitro compared with those from CCR9(-/-) mice. TNF-α or TGF-β1 antagonism attenuated CCR9(+) macrophage-induced HSC activation. Furthermore, C-C motif chemokine ligand (CCL) 25 mediated migration and, to a lesser extent, activation of HSCs in vitro.
  
  
  CONCLUSIONS
  Accumulated CD11b(+) macrophages are critical for activating HSCs through the CCR9/CCL25 axis and therefore promote liver fibrosis. CCR9 antagonism might be a novel therapeutic target for liver fibrosis.
authors: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
authors: Po-Sung
authors: PS
authors: Chu
authors: Nobuhiro
authors: N
authors: Nakamoto
authors: Hirotoshi
authors: H
authors: Ebinuma
authors: Shingo
authors: S
authors: Usui
authors: Keita
authors: K
authors: Saeki
authors: Atsuhiro
authors: A
authors: Matsumoto
authors: Yohei
authors: Y
authors: Mikami
authors: Kazuo
authors: K
authors: Sugiyama
authors: Kengo
authors: K
authors: Tomita
authors: Takanori
authors: T
authors: Kanai
authors: Hidetsugu
authors: H
authors: Saito
authors: Toshifumi
authors: T
authors: Hibi
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000097:blood*; D002252:Carbon Tetrachloride Poisoning; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000150:complications; D019742:Chemokines, CC / Q000502:physiology*; D004195:Disease Models, Animal; D055166:Hepatic Stellate Cells / Q000502:physiology*; D008103:Liver Cirrhosis / Q000209:etiology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D054388:Receptors, CCR / Q000172:deficiency / Q000502:physiology*
chemical_list: C119309:CC chemokine receptor 9; D039481:CD11b Antigen; C491541:Ccl25 protein, mouse; D019742:Chemokines, CC; D054388:Receptors, CCR