title: A two-dimensional proteome map of the aflatoxigenic fungus Aspergillus flavus.
abstract: The filamentous fungus Aspergillus flavus is an opportunistic soil-borne pathogen that produces aflatoxins, the most potent naturally occurring carcinogenic compounds known. This work represents the first gel-based profiling analysis of A. flavus proteome and establishes a 2D proteome map. Using 2DE and MALDI-TOF-MS/MS, we identified 538 mycelial proteins of the aflatoxigenic strain NRRL 3357, the majority of which were functionally annotated as related to various cellular metabolic and biosynthetic processes. Additionally, a few enzymes from the aflatoxin synthesis pathway were also identified.
authors: Mississippi State Chemical Laboratory, Mississippi State University, Mississippi State, MS, USA.
authors: Olga
authors: O
authors: Pechanova
authors: Tibor
authors: T
authors: Pechan
authors: Jose M
authors: JM
authors: Rodriguez
authors: W Paul
authors: WP
authors: Williams
authors: Ashli E
authors: AE
authors: Brown
mesh_terms: D000348:Aflatoxins / Q000378:metabolism; D001231:Aspergillus flavus / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005656:Fungal Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000348:Aflatoxins; D005656:Fungal Proteins; D020543:Proteome