title: The simultaneous occurrence of human norovirus and hepatitis E virus in a Norway rat (Rattus norvegicus).
abstract: Wild rats can be reservoirs and vectors for several human pathogens. An initial RT-PCR screening of the intestinal contents of Norway rats trapped in the sewer system of Copenhagen, Denmark, for caliciviruses revealed the presence of a human norovirus in one of 11 rodents. Subsequent phylogenetic analysis of the ~4.0-kb 3'-terminus of the norovirus genome resulted in the identification of a recombinant GI.b/GI.6 strain. The simultaneous detection of hepatitis E virus-like particles in the feces of this rat by transmission electron microscopy was confirmed by RT-PCR and sequence determination, resulting in the identification of a novel rat hepatitis E virus.
authors: Institute for Microbiology, Technische Universität Dresden, Dresden, Germany. sandro.wolf@tu-dresden.de
authors: Sandro
authors: S
authors: Wolf
authors: Jochen
authors: J
authors: Reetz
authors: Reimar
authors: R
authors: Johne
authors: Ann-Charlotte
authors: AC
authors: Heiberg
authors: Samuel
authors: S
authors: Petri
authors: Hanna
authors: H
authors: Kanig
authors: Rainer G
authors: RG
authors: Ulrich
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D060085:Coinfection / Q000662:veterinary* / Q000821:virology; D003718:Denmark; D005243:Feces / Q000821:virology; D005260:Female; D016752:Hepatitis E virus / Q000302:isolation & purification*; D008297:Male; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D029322:Norovirus / Q000302:isolation & purification*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012376:Rodent Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral