title: Imaging tumor cell movement in vivo.
abstract: This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.
authors: Albert Einstein College of Medicine, Bronx, New York.|These authors contributed equally to this work.
authors: David
authors: D
authors: Entenberg
authors: Albert Einstein College of Medicine, Bronx, New York.|These authors contributed equally to this work.
authors: Dmitriy
authors: D
authors: Kedrin
authors: Albert Einstein College of Medicine, Bronx, New York.
authors: Jeffrey
authors: J
authors: Wyckoff
authors: London Research Institute, London, United Kingdom.
authors: Erik
authors: E
authors: Sahai
authors: Albert Einstein College of Medicine, Bronx, New York.
authors: John
authors: J
authors: Condeelis
authors: Albert Einstein College of Medicine, Bronx, New York.
authors: Jeffrey E
authors: JE
authors: Segall
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D008264:Macrophages / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D051381:Rats