title: Suppression of LPS-induced inflammatory responses by inflexanin B in BV2 microglial cells.
abstract: Microglia are a type of resident macrophage that functions as an inflammation modulator in the central nervous system. Over-activation of microglia by a range of stimuli disrupts the physiological homeostasis of the brain, and induces inflammatory response and degenerative processes, such as those implicated in neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. Therefore, we investigated the possible anti-inflammatory mechanisms of inflexanin B in murine microglial BV2 cells. Lipopolysaccharide (LPS) activated BV2 cells and induced the production of pro-inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), and cytokines (interleukins-1β and -6, and tumour necrosis factor α). The LPS-induced production of pro-inflammatory mediators was associated with the enhancement of nuclear factor-kappaB (NF-κB) nuclear translocation and the activation of mitogen-activated protein kinase (MAPK) including ERK1/2 and JNK. Conversely, pretreatment of cells with inflexanin B (10 and 20 μg/mL) significantly reduced the production of pro-inflammatory mediators. This was accompanied with the reduced nuclear translocation of NF-κB and reduced activation of MAPKs. These results suggest that inflexanin B attenuated the LPS-induced inflammatory process by inhibiting the activation of NF-κB and MAPKs.
authors: Environmental Toxico-Genomic & Proteomic Center, College of Medicine, Korea University, Seoul 136-701, Korea.
authors: Ji-Youn
authors: JY
authors: Lim
authors: Donggeun
authors: D
authors: Sul
authors: Bang Yeon
authors: BY
authors: Hwang
authors: Kwang Woo
authors: KW
authors: Hwang
authors: Ki-Yeol
authors: KY
authors: Yoo
authors: So-Young
authors: SY
authors: Park
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D045786:Diterpenes, Kaurane / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D024202:Electrophoretic Mobility Shift Assay; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000276:immunology / Q000378:metabolism*; D037602:Isodon / Q000737:chemistry; D008070:Lipopolysaccharides / Q000633:toxicity*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D015394:Molecular Structure; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D035261:Plant Components, Aerial / Q000737:chemistry; D021381:Protein Transport
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D045786:Diterpenes, Kaurane; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; C583089:inflexanin B; D009569:Nitric Oxide; D015232:Dinoprostone