title: Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis.
abstract: Cell polarity plays an important role in tissue morphogenesis; however, the mechanisms of polarity and their role in mammalian development are still poorly understood. We show here that membrane-associated guanylate kinase protein Dlg5 is required for proper branching morphogenesis and progenitor differentiation in mammalian lung. We found that during lung development Dlg5 functions as an apical-basal polarity protein, which is necessary for the apical maintenance of atypical protein kinase C (aPKC). These results identify Dlg5 as a regulator of apical polarity complexes and uncover the critical function of Dlg5 in branching morphogenesis and differentiation of lung progenitor cells.
authors: Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, C3-168, Seattle, WA 98109, USA. nechipot@ohsu.edu
authors: Tamilla
authors: T
authors: Nechiporuk
authors: Olga
authors: O
authors: Klezovitch
authors: Liem
authors: L
authors: Nguyen
authors: Valeri
authors: V
authors: Vasioukhin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D016764:Cell Polarity / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005455:Fluorescent Antibody Technique; D051528:Guanylate Kinases / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D046228:Microarray Analysis; D009024:Morphogenesis / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism*; D013234:Stem Cells / Q000502:physiology*
chemical_list: D017931:DNA Primers; D008565:Membrane Proteins; C115015:PKC-3 protein; D011493:Protein Kinase C; C523873:Dlg5 protein, mouse; D051528:Guanylate Kinases