title: The persistence of biofilm-associated antibiotic resistance of Staphylococcus aureus isolated from clinical bovine mastitis cases in Australia.
abstract: The aim of this investigation was to determine the persistence of biofilm-associated antibiotic resistance developed by methicillin-sensitive Staphylococcus aureus (MSSA), of different capsular types, during biofilm formation. Because of superiority of the tissue culture plate (TCP) over the Congo Red Agar (CRA) method for measuring biofilm formation, it was used to determine the persistence of the antibiotic resistance developed by the isolates in biofilms. The antibiotic resistance was found to persist for 3-4 wk post-propagation as planktonic subcultures. Interestingly, some strains even developed resistance to vancomycin and/or teicoplanin. However, no association of either biofilm formation or persistent antibiotic resistance with the major capsular phenotype was observed. These observations highlight the potential significance of (a) determining the antibiograms of S. aureus subcultured from biofilms developed in vitro using the TCP method as well as from planktonic cultures for formulation of an optimal therapeutic strategy, and (b) continuing to identify predominant non-capsular antigens contributing to biofilm formation, regardless of the capsular phenotype for the development of an effective potentially broad-spectrum vaccine for prevention of bovine mastitis caused by S. aureus.
authors: School of Biomedical Sciences, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University Bentley, Perth, Western Australia, Australia, 6102.
authors: Charlene
authors: C
authors: Babra
authors: Jully G
authors: JG
authors: Tiwari
authors: Gerald
authors: G
authors: Pier
authors: Thi Ha
authors: TH
authors: Thein
authors: Raju
authors: R
authors: Sunagar
authors: Srinivasaiah
authors: S
authors: Sundareshan
authors: Shrikrishna
authors: S
authors: Isloor
authors: Nagendra R
authors: NR
authors: Hegde
authors: Sharon
authors: S
authors: de Wet
authors: Margaret
authors: M
authors: Deighton
authors: Justine
authors: J
authors: Gibson
authors: Paul
authors: P
authors: Costantino
authors: John
authors: J
authors: Wetherall
authors: Trilochan
authors: T
authors: Mukkur
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001315:Australia; D016667:Bacterial Capsules / Q000235:genetics; D001426:Bacterial Proteins; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002417:Cattle; D024881:Drug Resistance, Bacterial; D005838:Genotype; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C425726:Bap protein, Staphylococcus aureus