title: In situ preparation of gold nanoparticle-loaded lysozyme-dextran nanogels and applications for cell imaging and drug delivery.
abstract: An effective, green, and facile approach to synthesize gold nanoparticle-loaded protein-polysaccharide nanogels was developed in this study. Biocompatible gold nanoparticle-loaded lysozyme-dextran (Au@Lys-Dex) nanogels were produced using lysozyme-dextran nanogels as reducing and stabilizing agents. Lysozyme-dextran nanogels have a size of about 200 nm and a structure of lysozyme core and dextran shell. At pH around 4, AuCl4(-) ions are attracted and locally enriched by lysozyme due to the electrostatic and coordination interactions. When the solution is under UV irradiation, the AuCl4(-) ions are reduced to gold nanoparticles in situ by solvated electrons and reactive radicals produced from aromatic amino acid residues in the lysozyme. The produced gold nanoparticles with a size of about 8 nm are trapped inside the nanogels and the Au@Lys-Dex nanogels are well dispersible by virtue of the dextran shell. Antitumor drug, doxorubicin, can be loaded effectively inside Au@Lys-Dex nanogels via diffusion. In vitro study demonstrates the doxorubicin loaded Au@Lys-Dex nanogels have the same antitumor activity as free doxorubicin. The nanogels can be used as a contrasting agent in optical cell imaging, in which direct visual images of the subcellular distributions of the gold nanoparticles and the released doxorubicin are presented synchronously. The dual functional drug loaded Au@Lys-Dex nanogels are a promising system for simultaneous drug delivery and biomedical imaging.
authors: State Key Laboratory of Molecular Engineering of Polymer and Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors: Huanxin
authors: H
authors: Cai
authors: Ping
authors: P
authors: Yao
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation; D003911:Dextrans / Q000008:administration & dosage / Q000737:chemistry*; D003952:Diagnostic Imaging; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D016503:Drug Delivery Systems; D005782:Gels / Q000138:chemical synthesis / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009113:Muramidase / Q000008:administration & dosage / Q000737:chemistry*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D011094:Polyethyleneimine / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D003911:Dextrans; D004337:Drug Carriers; D005782:Gels; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D006046:Gold; D004317:Doxorubicin; D011094:Polyethyleneimine; D009113:Muramidase