title: Cytotoxic and antimalarial amaryllidaceae alkaloids from the bulbs of Lycoris radiata.
abstract: Phytochemical investigation of the 80% ethanol extract of the bulbs of  Lycoris radiata resulted in the isolation of five new Amaryllidaceae alkaloids: (+)-5,6-dehydrolycorine (1), (+)-3α,6β-diacetyl-bulbispermine (2), (+)-3α-hydroxy-6β-acetyl- bulbispermine (3), (+)-8,9-methylenedioxylhomolycorine-N-oxide (5), and 5,6-dihydro-5- methyl-2-hydroxyphenanthridine (7), together with two known compounds, (+)-3α-methoxy- 6β-acetylbulbispermine (4) and (+)-homolycorine- N-oxide (6). Structural elucidation of all the compounds were performed by spectral methods such as 1D and 2D (1H-1H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. Alkaloid 1 showed potent cytotoxicity against astrocytoma and glioma cell lines (CCF-STTG1, CHG-5, SHG-44, and U251), as well as HL-60, SMMC-7721, and W480 cell lines with IC(50) values of 9.4-11.6 μM. Additonally, compound 1 exhibited antimalarial activity with IC(50) values of 2.3 μM for D-6 strain and 1.9 μM for W-2 strain of  Plasmodium falciparum.
authors: Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Bin
authors: B
authors: Hao
authors: Shu-Fang
authors: SF
authors: Shen
authors: Qing-Jie
authors: QJ
authors: Zhao
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D018922:HL-60 Cells; D006801:Humans; D031426:Lycoris / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects
chemical_list: D047151:Amaryllidaceae Alkaloids; D000962:Antimalarials; D010936:Plant Extracts