title: Pumpless steady-flow microfluidic chip for cell culture.
abstract: The current research engineered a pumpless energy-efficient microfluidic perfusion cell culture chip that works by modifying the basic gravity-driven siphon flow using an intravenous (IV) infusion set as a conventional, inexpensive, and sterile tool. The IV set was modified to control the constant hydrostatic head difference, thereby maintaining the steady flow rate medium perfusion. The micro-bioreactor chip demonstrated flexibility in controlling a wide range of flow rates from 0.1 to 10ml/min, among which 1- and 5-ml/min flow rates were examined as suitable shear flows for long-term dermal fibroblast cell culture, paving the way for artificial skin development.
authors: Department of Bionanotechnology, Gachon University, Gyeonggi-do 461-701, Republic of Korea.
authors: Mohana
authors: M
authors: Marimuthu
authors: Sanghyo
authors: S
authors: Kim
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods