title: Cellulose-ethylenediaminetetraacetic acid conjugates protect mammalian cells from bacterial cells.
abstract: Cellulose-ethylenediaminetetraacetic acid (EDTA) conjugates were synthesized by the esterification of cellulose with ethylenediaminetetraacetic dianhydride (EDTAD). The new materials provided potent antimicrobial activities against Staphylococcus aureus (S. aureus, Gram-positive bacteria) and Pseudomonas aeruginosa (P. aeruginosa, Gram-negative bacteria), and inhibited the formation of bacterial biofilms. The biocompatibility of the new cellulose-EDTA conjugates was evaluated with mouse skin fibroblasts for up to 14 days. SEM observation and DNA content analysis suggested that the new materials sustained the viability of fibroblast cells. Moreover, in mouse skin fibroblast-bacteria co-culture systems, the new cellulose-EDTA conjugates prevented bacterial biofilm formation and protected the mammalian cells from the bacterial cells for at least one day.
authors: Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01842, USA.
authors: Jie
authors: J
authors: Luo
authors: Wei
authors: W
authors: Lv
authors: Ying
authors: Y
authors: Deng
authors: Yuyu
authors: Y
authors: Sun
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry / Q000494:pharmacology*; D004492:Edetic Acid / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000382:microbiology; D051379:Mice; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012867:Skin / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D002482:Cellulose; D004492:Edetic Acid