title: [Study on the extraction of mycelium polysaccharides from selenium-rich Morchella esculenta with neutral proteinase method].
abstract: OBJECTIVE
  To study the extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta with neutral proteinase for increasing of yield.
  
  
  METHODS
  On the basis of single factor tests, orthogonal experiment design was applied to analyze the influence of factors including enzyme dosage, enzymolysis time, enzymolysis temperature and material/liquid ratio on the extraction rate of the polysaccharides.
  
  
  RESULTS
  The optimal extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta was as follows: enzyme dosage was 1.5%, enzymolysis time was 2 h, enzymolysis temperature was 40 degrees C, and material/liquid ratio was 1:15. The extraction rate of polysaccharide was 11.26% under the extraction conditions.
  
  
  CONCLUSIONS
  The process is simple, stable and practicable, and can be used for the extraction of mycelium polysaccharides from selenium-rich Morchella esculenta.
authors: School of Life Science, Anhui Science and Technology University, Fengyang 233100, China. sunyujun320@yahoo.com.cn
authors: Yu-jun
authors: YJ
authors: Sun
authors: Zheng-peng
authors: ZP
authors: Li
authors: Zhong-you
authors: ZY
authors: Ma
authors: Song-hua
authors: SH
authors: Wang
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D010450:Endopeptidases / Q000008:administration & dosage / Q000378:metabolism*; D005285:Fermentation; D025282:Mycelium / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012643:Selenium; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D011134:Polysaccharides; D010450:Endopeptidases; D012643:Selenium