title: Determination of robenidine residues in chicken muscle by high performance liquid chromatography with ultraviolet detection.
abstract: A simple, robust and reliable method for the determination of residual robenidine in chicken muscle using high performance liquid chromatography with ultraviolet (UV) detection was developed and validated according to the Codex Alimentarius Commission guidelines. Chicken muscle was extracted by acetonitrile/formic acid (98:2, v/v) and defatted with hexane. Analytes were isocratically separated on a Luna C18 column (4.6 × 150 mm, 5 μm) using 70 % methanol in water containing 0.1 % trifluoroacetic acid at a flow rate of 1.0 mL/min at 30 °C. UV detection was performed at 312 nm. The method was validated by assessing performance parameters including selectivity, linearity, limit of quantification (LOQ), precision, accuracy, recovery, stability and robustness. A calibration curve that was constructed over 0.05-0.5 μg/g showed correlation coefficients of more than 0.999. The intra- and inter-day precisions (as coefficient of variation) were 1.45-3.32 and 2.63-4.99 %, respectively. The intra- and inter-day accuracies were 99.4-105.3 and 98.3-101.6 %, respectively. The recoveries were in the range of 76.6-81.8 % and the LOQ was 0.05 μg/g. The developed method showed suitable performance for the determination of robenidine residues in chicken muscle.
authors: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul, 156-756, South Korea.
authors: Hyesun
authors: H
authors: Yeom
authors: Dong-Hyug
authors: DH
authors: Yang
authors: Joon Hyuk
authors: JH
authors: Suh
authors: Han Young
authors: HY
authors: Eom
authors: Unyong
authors: U
authors: Kim
authors: Junghyun
authors: J
authors: Kim
authors: Seul Gi
authors: SG
authors: Lee
authors: Hyun-Deok
authors: HD
authors: Cho
authors: Sang Beom
authors: SB
authors: Han
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D018482:Muscle, Skeletal / Q000737:chemistry*; D015203:Reproducibility of Results; D012370:Robenidine / Q000032:analysis* / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000592:standards
chemical_list: D012370:Robenidine