title: Effects of anesthetic isoflurane and desflurane on human cerebrospinal fluid Aβ and τ level.
abstract: BACKGROUND
  Accumulation of β-amyloid protein (Aβ) and tau protein is the main feature of Alzheimer disease neuropathogenesis. Anesthetic isoflurane, but not desflurane, may increase Aβ levels in vitro and in animals. Therefore, we set out to determine the effects of isoflurane and desflurane on cerebrospinal fluid (CSF) levels of Aβ and tau in humans.
  
  
  METHODS
  The participants were assigned into spinal anesthesia (N=35), spinal plus desflurane anesthesia (N=33), or spinal plus isoflurane anesthesia (N=38) group by randomization using computer-generated lists. Pre- and postoperative human CSF samples were obtained through an inserted spinal catheter. The levels of Aβ (Aβ40 and Aβ42) and total tau in the CSF were determined.
  
  
  RESULTS
  Here, we show that isoflurane, but not desflurane, was associated with an increase in human CSF Aβ40 levels (from 10.90 to 12.41 ng/ml) 24 h after the surgery under anesthesia compared to spinal anesthesia (from 11.59 to 11.08 ng/ml), P=0.022. Desflurane, but not isoflurane, was associated with a decrease in Aβ42 levels 2 h after the surgery under anesthesia (from 0.39 to 0.35 ng/ml) compared to spinal anesthesia (from 0.43 to 0.44 ng/ml), P=0.006. Isoflurane and desflurane did not significantly affect the tau levels in human CSF.
  
  
  CONCLUSIONS
  These studies have established a system to study the effects of anesthetics on human biomarkers associated with Alzheimer disease and cognitive dysfunction. These findings have suggested that isoflurane and desflurane may have different effects on human CSF Aβ levels.
authors: Department of Anesthesia, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
authors: Bin
authors: B
authors: Zhang
authors: Ming
authors: M
authors: Tian
authors: Hui
authors: H
authors: Zheng
authors: Yu
authors: Y
authors: Zhen
authors: Yun
authors: Y
authors: Yue
authors: Tianzuo
authors: T
authors: Li
authors: Shuren
authors: S
authors: Li
authors: Edward R
authors: ER
authors: Marcantonio
authors: Zhongcong
authors: Z
authors: Xie
mesh_terms: D000005:Abdomen / Q000601:surgery; D000368:Aged; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D000769:Anesthesia, Inhalation; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D016063:Blood Loss, Surgical / Q000706:statistics & numerical data; D000077335:Desflurane; D005260:Female; D006801:Humans; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D019637:Orthopedic Procedures; D010446:Peptide Fragments / Q000134:cerebrospinal fluid; D012959:Socioeconomic Factors; D013514:Surgical Procedures, Operative; D016875:tau Proteins / Q000134:cerebrospinal fluid*
chemical_list: D016229:Amyloid beta-Peptides; D018685:Anesthetics, Inhalation; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); D016875:tau Proteins; D000077335:Desflurane; D007530:Isoflurane