title: Different assembly of acid and salt tolerance response in two dairy Listeria monocytogenes wild strains.
abstract: A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v] NaCl), a proteomic approach was used. The ATR and OTR of two L. monocytogenes cheese dairy isolates (strain T8, serotype 4b and A9, serotype 1/2b or 3b) were determined. The proteomes of the adapted and non-adapted cultures were evaluated by 2-DE. One strain displayed an ATR, but not an OTR and the other displayed an OTR, but not an ATR. The ATR positive strain showed the over-production of proteins related with protein synthesis, protein folding, attainment of reduction power, ribose production and cell wall. In contrast, in the OTR-positive-strain proteins related with glycolysis, general stress and detoxification were identified.
authors: Faculdade de Ciências e de Tecnologia (FCT), Centro de Biomedicina Molecular e Estrutural (IBB), Universidade do Algarve, IBB-CBME, Campus de Gambelas, 8000-117 Faro, Portugal.
authors: Jessie
authors: J
authors: Melo
authors: Peter William
authors: PW
authors: Andrew
authors: Maria Leonor
authors: ML
authors: Faleiro
mesh_terms: D000143:Acids; D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000032:analysis; D002611:Cheese / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D020543:Proteome / Q000032:analysis; D055049:Salt Tolerance
chemical_list: D000143:Acids; D001426:Bacterial Proteins; D020543:Proteome