title: Dispersion management in two-photon microscopy by using diffractive optical elements.
abstract: We demonstrate efficient generation of wide-field fluorescence signals in two-photon microscopy exploiting diffractive optical elements and short pulses by using a dispersion-compensated beam delivery optics module. Computer-generated holograms are codified onto a phase-only spatial light modulator, which allows for arbitrary single-shot patterning of the sample. Spatiotemporal shaping of the pulse is mandatory to overcome spatial chirp and pulse-front tilt effects that spread both in space and time the irradiance patterns, thus limiting not only the spatial resolution but also the signal-to-noise ratio in two-photon microscopy. By using a multipass amplifier delivering 30 fs, 0.8 mJ pulses at 1 kHz repetition rate, we experimentally demonstrated arbitrary single-shot fluorescence irradiance patterns in Rhodamine B.
authors: Institut de Noves Tecnologies de la Imatge (INIT), Universitat Jaume I, Castelló 12080, Spain.
authors: Jorge
authors: J
authors: Pérez-Vizcaíno
authors: Omel
authors: O
authors: Mendoza-Yero
authors: Gladys
authors: G
authors: Mínguez-Vega
authors: Raúl
authors: R
authors: Martínez-Cuenca
authors: Pedro
authors: P
authors: Andrés
authors: Jesús
authors: J
authors: Lancis
mesh_terms: D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D055620:Optical Phenomena