title: Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus.
abstract: Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.
authors: Laboratory Science Division, International Vaccine Institute, Seoul 151-919, Republic of Korea.
authors: Byoung-Shik
authors: BS
authors: Shim
authors: Jung-Ah
authors: JA
authors: Choi
authors: Ho-Hyun
authors: HH
authors: Song
authors: Sung-Moo
authors: SM
authors: Park
authors: In Su
authors: IS
authors: Cheon
authors: Ji-Eun
authors: JE
authors: Jang
authors: Sun Je
authors: SJ
authors: Woo
authors: Chung Hwan
authors: CH
authors: Cho
authors: Min-Suk
authors: MS
authors: Song
authors: Hyemi
authors: H
authors: Kim
authors: Kyung Joo
authors: KJ
authors: Song
authors: Jae Myun
authors: JM
authors: Lee
authors: Suhng Wook
authors: SW
authors: Kim
authors: Dae Sub
authors: DS
authors: Song
authors: Young Ki
authors: YK
authors: Choi
authors: Jae-Ouk
authors: JO
authors: Kim
authors: Huan Huu
authors: HH
authors: Nguyen
authors: Dong Wook
authors: DW
authors: Kim
authors: Young Yil
authors: YY
authors: Bahk
authors: Cheol-Heui
authors: CH
authors: Yun
authors: Man Ki
authors: MK
authors: Song
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000235:genetics; D000286:Administration, Sublingual; D000818:Animals; D057134:Antibodies, Neutralizing / Q000032:analysis / Q000097:blood; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D002772:Cholera Toxin / Q000008:administration & dosage / Q000235:genetics; D004195:Disease Models, Animal; D058501:Enzyme-Linked Immunospot Assay; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D018928:Immunity, Mucosal; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D044967:Serum / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D019562:Viral Load
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic; D002772:Cholera Toxin