title: Preparation and characterizations of polyclonal antibodies against STAR protein QKI7b.
abstract: Quaking (QKI) proteins are important regulators of RNA metabolism and cellular signal transduction. Recent studies have shown that isoforms of QKI proteins, which include QKI5/6/7/7b in human cells, play important roles in the development of neurological diseases and human cancers. In comparison with QKI5/6/7, however, there are little data on QKI7b due to lack of specific antibodies. Here, we reported the preparation and initial characterizations of polyclonal antibodies against human QKI7b. Utilizing a chemically synthesized C-terminal peptide fragment of human QKI7b, we raised two preparations of rabbit antiserum. We found that these antibodies were able to recognize human QKI7b, but not QKI5/6/7. Our immunofluorescence staining showed that in LO2 hepatocytes, QKI7b localizes predominantly in the perinuclear cytoplasm and less abundantly in the nucleus. In clinical samples, we showed that like QKI5/6/7 proteins, QKI7b protein was also significantly downregulated in most human colorectal cancer tissues. These antibodies, therefore, might be useful in future functional studies of QKI7b.
authors: School of Biomedical Sciences, Huaqiao University, Quanzhou, China, 362021.
authors: Qiuyan
authors: Q
authors: Liu
authors: Yu
authors: Y
authors: Luo
authors: Xiaotong
authors: X
authors: Li
authors: Li
authors: L
authors: Yuan
authors: Ruian
authors: R
authors: Xu
authors: James Y
authors: JY
authors: Yang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D015179:Colorectal Neoplasms / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006433:Hemocyanins / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D016601:RNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000906:Antibodies; C490832:QKI protein, human; D016601:RNA-Binding Proteins; D006433:Hemocyanins