title: Immunohistochemical characterization of type II pneumocyte proliferation after challenge with type I porcine reproductive and respiratory syndrome virus.
abstract: The aim of this study was to characterize histologically and immunohistochemically the lung lesions developing in growing pigs, 10 and 21 days after experimental challenge with a field strain of porcine reproductive and respiratory syndrome virus (PRRSV). Lung lesions were scored for (1) pneumocyte hypertrophy and hyperplasia, (2) septal mononuclear infiltration, (3) intra-alveolar necrotic debris, (4) intra-alveolar inflammatory cell accumulation and (5) perivascular inflammatory cell accumulation. Immunohistochemistry was performed using antibodies specific for cytokeratin, Ki67, thyroid transcription factor (TTF)-1, the myelomonocytic marker MAC387 and PRRSV. Anti-TTF-1 identified type II pneumocytes and there was marked proliferation of these cells compared with control lung (P <0.05). Anti-cytokeratin labelled type I and II pneumocytes as well as bronchial epithelial cells; however, this labelling was not suitable for cell counting purposes. There was a correlation between lesion severity and the number of cells expressing Ki67 (P <0.05).
authors: Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent István University, Budapest, Hungary. balka.gyula@aotk.szie.hu
authors: G
authors: G
authors: Balka
authors: A
authors: A
authors: Ladinig
authors: M
authors: M
authors: Ritzmann
authors: A
authors: A
authors: Saalmüller
authors: W
authors: W
authors: Gerner
authors: T
authors: T
authors: Käser
authors: C
authors: C
authors: Jakab
authors: M
authors: M
authors: Rusvai
authors: H
authors: H
authors: Weißenböck
mesh_terms: D056809:Alveolar Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000473:pathology; D019316:Porcine respiratory and reproductive syndrome virus; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
keywords: PRRSV; TTF-1; pig; type II pneumocyte