title: Probiotics and colostrum/milk differentially affect neonatal humoral immune responses to oral rotavirus vaccine.
abstract: Breast milk (colostrum [col]/milk) components and gut commensals play important roles in neonatal immune maturation, establishment of gut homeostasis and immune responses to enteric pathogens and oral vaccines. We investigated the impact of colonization by probiotics, Lactobacillus rhamnosus GG (LGG) and Bifidobacterium lactis Bb12 (Bb12) with/without col/milk (mimicking breast/formula fed infants) on B lymphocyte responses to an attenuated (Att) human rotavirus (HRV) Wa strain vaccine in a neonatal gnotobiotic pig model. Col/milk did not affect probiotic colonization in AttHRV vaccinated pigs. However, unvaccinated pigs fed col/milk shed higher numbers of probiotic bacteria in feces than non-col/milk fed colonized controls. In AttHRV vaccinated pigs, col/milk feeding with probiotic treatment resulted in higher mean serum IgA HRV antibody titers and intestinal IgA antibody secreting cell (ASC) numbers compared to col/milk fed, non-colonized vaccinated pigs. In vaccinated pigs without col/milk, probiotic colonization did not affect IgA HRV antibody titers, but serum IgG HRV antibody titers and gut IgG ASC numbers were lower, suggesting that certain probiotics differentially impact HRV vaccine responses. Our findings suggest that col/milk components (soluble mediators) affect initial probiotic colonization, and together, they modulate neonatal antibody responses to oral AttHRV vaccine in complex ways.
authors: Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691-4096, USA. chattha.2@osu.edu
authors: Kuldeep S
authors: KS
authors: Chattha
authors: Anastasia N
authors: AN
authors: Vlasova
authors: Sukumar
authors: S
authors: Kandasamy
authors: Malak A
authors: MA
authors: Esseili
authors: Christine
authors: C
authors: Siegismund
authors: Gireesh
authors: G
authors: Rajashekara
authors: Linda J
authors: LJ
authors: Saif
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology* / Q000382:microbiology; D001402:B-Lymphocytes / Q000276:immunology; D001644:Bifidobacterium / Q000276:immunology / Q000502:physiology; D015169:Colony Count, Microbial; D003126:Colostrum / Q000737:chemistry / Q000276:immunology*; D005243:Feces / Q000382:microbiology; D005260:Female; D005856:Germ-Free Life; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D052201:Lacticaseibacillus rhamnosus / Q000276:immunology / Q000502:physiology; D008895:Milk, Human / Q000737:chemistry / Q000276:immunology*; D023421:Models, Animal; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control; D022243:Rotavirus Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D034421:Sus scrofa / Q000276:immunology / Q000382:microbiology; D016212:Transforming Growth Factor beta / Q000097:blood; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; D016212:Transforming Growth Factor beta; D014613:Vaccines, Attenuated