title: Calibration of a digital in-line holographic microscopy system: depth of focus and bioprocess analysis.
abstract: Digital in-line holographic microscopy (DIHM) allows access to both intensity and phase information with conventional microscopic lateral resolutions. Such imaging techniques can, however, be used to increase the depth of focus compared to conventional compound microscopes. We present a simple DIHM capable of imaging weakly scattering 10 μm diameter microspheres as well as Hs578T cells over a depth of 1 mm; i.e., we demonstrate an increase by a factor of 100 over the depth of focus of a conventional microscope.
authors: The Callan Institute, Department of Electronic Engineering, National University of Ireland Maynooth, Maynooth, Co. Kildare, Ireland.
authors: James P
authors: JP
authors: Ryle
authors: Susan
authors: S
authors: McDonnell
authors: Brian
authors: B
authors: Glennon
authors: John T
authors: JT
authors: Sheridan
mesh_terms: D000465:Algorithms; D001702:Biopharmaceutics / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D045744:Cell Line, Tumor; D004345:Drug Industry / Q000379:methods; D004867:Equipment Design; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics; D012542:Scattering, Radiation