title: Concentrations of clindamycin hydrochloride in homogenates of normal dog skin when administered at two oral dosage regimens.
abstract: BACKGROUND
  Clindamycin is frequently used for the treatment of bacterial pyoderma.
  
  
  OBJECTIVE
  To compare the pharmacokinetics of clindamycin in whole skin homogenates of normal dogs when administered orally at two dosage regimens (5.5 mg/kg BW, twice daily and 11 mg/kg BW, once daily).
  
  
  METHODS
  Skin biopsies were obtained from six laboratory beagles before, 3, 6 and 12 h after the first and the fifth dose of clindamycin at the former regimen, as well as before, 3, 6, 12 and 24 h after the first and third dose at the latter regimen. Tissue was homogenized and clindamycin concentrations were measured by reverse-phase high-performance liquid chromatography coupled with mass spectrometry. Results were analyzed using Student's t-test at a level of significance of 0.05.
  
  
  RESULTS
  Maximal concentration and area under the concentration-time curve, but not their relevant dose-normalized values, were higher at the dosage regimen of 11 mg/kg BW, once daily than at 5.5 mg/kg BW twice daily.
  
  
  CONCLUSIONS
  The pharmacokinetic profile of clindamycin is at least equal, if not better, when this antimicrobial is administered at 11 mg/kg BW, once daily.
authors: Department of Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
authors: Manolis N
authors: MN
authors: Saridomichelakis
authors: Labrini V
authors: LV
authors: Athanasiou
authors: Manolis K
authors: MK
authors: Chatzis
authors: Michel
authors: M
authors: Salame
authors: Vassilis
authors: V
authors: Katsoudas
authors: Ioannis S
authors: IS
authors: Pappas
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D002981:Clindamycin / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D018592:Cross-Over Studies; D004285:Dogs / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D012867:Skin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin