title: The effect of granulocyte and monocyte adsorptive apheresis on serum cytokine levels in patients with ulcerative colitis.
abstract: Granulocyte and monocyte adsorptive apheresis (GMA) with an Adacolumn has been reported to be effective as induction therapy in ulcerative colitis (UC). However, the effects of GMA on serial changes in cytokine levels have not been well characterized. We therefore, investigated cytokine levels in UC patients before and after treatment with GMA. A total of 16 patients with active UC, 10 men, and six women, mean age, 42.6 years were included. Fourteen patients had total colitis and two patients had left-sided colitis. The study included nine patients with a chronic intermittent course, six with a chronic continuous course and one with a single episode. The duration of each GMA session was 60 min at a flow rate of 30 mL/min as per study protocol. Serum levels of 17 cytokines were determined simultaneously using a Bio-Plex suspension array system before and after treatment with GMA. Serum interleukin (IL)-10 and macrophage inflammatory protein-1β levels were increased significantly in UC patients after GMA treatment compared to pre-treatment levels (P < 0.05). In particular, GMA treatment caused a significant increase in serum IL-10 levels compared to pre-treatment in patients with total colitis or with a chronic intermittent UC course. In conclusion, this investigation showed that GMA was associated with a marked increase in serum level of the anti-inflammatory cytokine, IL-10. The rise in circulating IL-10 is interesting, and potentially a significant factor in the efficacy of GMA in patients with inflammatory bowel diseases.
authors: Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, Iwate Medical University, Morioka, Iwate 020-8505, Japan.
authors: Yosuke
authors: Y
authors: Toya
authors: Toshimi
authors: T
authors: Chiba
authors: Tomomi
authors: T
authors: Mizutani
authors: Kunihiko
authors: K
authors: Sato
authors: Satoshi
authors: S
authors: Kasugai
authors: Nozomi
authors: N
authors: Matsuda
authors: Shunsuke
authors: S
authors: Orikasa
authors: Sho
authors: S
authors: Shibata
authors: Yukito
authors: Y
authors: Abiko
authors: Risaburo
authors: R
authors: Akasaka
authors: Naoki
authors: N
authors: Yokoyama
authors: Shuhei
authors: S
authors: Oana
authors: Shigeru
authors: S
authors: Hirota
authors: Masaki
authors: M
authors: Endo
authors: Kazuyuki
authors: K
authors: Suzuki
mesh_terms: D000327:Adsorption; D000328:Adult; D001781:Blood Component Removal; D003093:Colitis, Ulcerative / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D009000:Monocytes / Q000378:metabolism*
chemical_list: D016207:Cytokines; D016753:Interleukin-10