title: Effects of soil organic matter and ageing on remediation of diesel-contaminated soil.
abstract: Bioremediation of diesel-contaminated soil was investigated for the effects of soil organic matter (SOM) and ageing time in two sets of experiments (Batch I and II, respectively). This study examined degradation efficiency in soil artificially contaminated with diesel oil (maximum total petroleum hydrocarbons (TPH) concentration of 9000 mg/kg soil). Batch I data showed that the values of the first-order degradation rate, k, were relatively high in the low-SOM soil batches. The quantity of SOM negatively correlated with the TPH degradation rates and with the total TPH degradation efficiency (%). Introduction of rhamnolipid to the soil proved to be a useful solution to resolve the problem of the residual TPH in the soil with high SOM. In Batch II, the k values decreased with the length of ageing time: 0.0245, 0.0128 and 0.0090 l/d in samples ST0 (freshly contaminated), ST38 (aged for 38 days) and ST101 (aged for 101 days), respectively. The TPH degradation efficiency (%) also decreased along with the ageing time. The research also applied molecular technology to analyse the bacterial community dynamics during the bioremediation course. Multivariate statistics based on terminal-restriction fragment length data indicated: 1) the soils with different SOM resulted in separate bacterial community structures, 2) ageing time created a variety of bacterial communities, 3) the bacterial community dynamics was associated with the hydrocarbon consumption. The SOM content in soils affected the TPH degradation rate and efficiency and the bacterial community structures. Aged soil is more difficult to remediate than freshly contaminated soil, and the resulting bacterial community was less dynamic and showed a lack of succession.
authors: Department of Safety Health and Environmental Engineering, Chung Hwa University of Medical Technology, No. 89 Wenhua 1st Street, Rende Shiang District, Tainan City 71703, Taiwan, ROC. graceliu@mail.hwai.edu.tw
authors: Pao-Wen Grace
authors: PW
authors: Liu
authors: Sih-Yu
authors: SY
authors: Wang
authors: Shen-Gzhi
authors: SG
authors: Huang
authors: Ming-Zhi
authors: MZ
authors: Wang
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D017931:DNA Primers; D052918:Environmental Restoration and Remediation; D005742:Gasoline; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012989:Soil Pollutants / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D005742:Gasoline; D012989:Soil Pollutants