title: [First molecular detection of Rhodococcus equi in a HIV/AIDS patient in Cuba].
abstract: BACKGROUND
  Rhodococcus equi is recognized as an emerging pathogen that causes important morbidity and mortality among immunocompromised patients.
  
  
  OBJECTIVE
  to confirm the presence of R. equi in pleural fluid through the restriction fragment length polymorphism technique.
  
  
  METHODS
  the pleural fluid sample from one AIDS patient with respiratory symptoms was used. Microbiologic culture, staining tests, phenotypic and biochemical tests and restriction fragment length polymorphism technique for the diagnosis of microorganism were performed.
  
  
  RESULTS
  the staining technique along with the phenotypic and biochemical tests provided the presumptive diagnosis of R. equi infection, which was further confirmed by the molecular techniques.
  
  
  CONCLUSIONS
  this paper reported the molecular detection of R. equi from one HIV/aids patient for the first time in Cuba. The results suggested that the molecular biology techniques could be used in the diagnosis and identification of R. equi.
authors: Instituto de Medicina Tropical "Pedro Kourí", La Habana, Cuba.
authors: Daniel
authors: D
authors: Salazar Rodríguez
authors: Teresa
authors: T
authors: Migdalia Reyes
authors: Francisco
authors: F
authors: Rodríguez Delgado
authors: Francisco
authors: F
authors: Bandera Tirado
authors: Angélica
authors: A
authors: Reyes Pérez
authors: Vilma Z
authors: VZ
authors: Medina Almenares
authors: C Jacobus H
authors: CJ
authors: de Waard
authors: Yaxsier
authors: Y
authors: de Armas Rodríguez
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods; D003462:Cuba; D015658:HIV Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D016929:Rhodococcus equi / Q000302:isolation & purification*