title: Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis.
abstract: Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e.g., C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8:0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids.
authors: Department of Plant Biology, Great Lakes Bioenergy Research Center Michigan State University, East Lansing, MI 48824, USA. henrik@msu.edu
authors: Henrik
authors: H
authors: Tjellström
authors: Merissa
authors: M
authors: Strawsine
authors: Jillian
authors: J
authors: Silva
authors: Edgar B
authors: EB
authors: Cahoon
authors: John B
authors: JB
authors: Ohlrogge
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D019730:Carbon-Sulfur Ligases / Q000235:genetics* / Q000378:metabolism; D031562:Cuphea / Q000201:enzymology / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D030821:Plants, Genetically Modified; D018087:Plastids / Q000201:enzymology / Q000235:genetics; D012639:Seeds / Q000235:genetics* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D005227:Fatty Acids; D014280:Triglycerides; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase; D019730:Carbon-Sulfur Ligases; C018749:long-chain-fatty-acid-(acyl-carrier-protein) ligase