title: [Function of transporter genes fscTI and fscTII in the biosynthetic cluster of candicidin/FR-008].
abstract: OBJECTIVE To investigate function of transporter genes fscTI and fscTII in the biosynthetic gene cluster of candicidin/FR-008.
  
  
  METHODS
  We constructed a plasmid pJTU4137 for disruption of transporter genes fscTI and fscTII by conjugation and homologous recombinant. The transporter genes were also PCR amplified and cloned into the high-copy plasmid pJTU1278 for overexpression in strain ZYJ-6 derived from Streptomyces sp. FR-008.
  
  
  RESULTS
  The disruption mutant LX10 was unable to produce candicidin and its analogues. Overexpression of FscTI and FscTII in ZYJ-6 caused a 1.5-fold increase in FR-008-III production compared with the control.
  
  
  CONCLUSIONS
  We confirmed that fscTI and fscTII are function as ATP dependent ATP binding cassetle (ABC) transporters in the biosynthetic gene cluster of FR-008. Furthermore, a positive example was provided for improving antibiotic production in other polyene producing strains based on the results that overexpression of fscTI and fscTI increased candicidin production.
authors: State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China. myown625@sjtu.edu.cn
authors: Xuan
authors: X
authors: Lei
authors: Lingxin
authors: L
authors: Kong
authors: Chen
authors: C
authors: Zhang
authors: Delin
authors: D
authors: You
authors: Zixin
authors: Z
authors: Deng
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002174:Candicidin / Q000096:biosynthesis*; D005810:Multigene Family; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002174:Candicidin; D000255:Adenosine Triphosphate