title: Physicochemical characterization of Acidiphilium sp. biofilms.
abstract: The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins.
authors: Bioctalysis, Instituto de Catálisis y Petroleoquímica, CSIC, 2 Marie Curie, 28049 Madrid, Spain.
authors: Moustafa
authors: M
authors: Malki
authors: Santiago
authors: S
authors: Casado
authors: María Francisca
authors: MF
authors: López
authors: Renaud
authors: R
authors: Caillard
authors: F Javier
authors: FJ
authors: Palomares
authors: José A
authors: JA
authors: Martín Gago
authors: Cristina
authors: C
authors: Vaz-Domínguez
authors: Angel
authors: A
authors: Cuesta
authors: Ricardo
authors: R
authors: Amils
authors: Víctor M
authors: VM
authors: Fernández
authors: Marisela
authors: M
authors: Velez
authors: Antonio L
authors: AL
authors: De Lacey
authors: David
authors: D
authors: Olea
mesh_terms: D041801:Acidiphilium / Q000502:physiology*; D018441:Biofilms; D006108:Graphite / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D056951:Photoelectron Spectroscopy
chemical_list: D006108:Graphite