title: Immune signalling responses in intestinal epithelial cells exposed to pathogenic Escherichia coli and lactic acid-producing probiotics.
abstract: Enterohaemorrhagic Escherichia coli O157:H7 and adherent-invasive Escherichia coli are two groups of enteric bacterial pathogens associated with haemorrhagic colitis and Crohn's Disease, respectively. Bacterial contact with host epithelial cells stimulates an immediate innate immune response designed to combat infection. In this study, immune responses of human epithelial cells to pathogens, either alone or in combination with probiotic bacteria were studied. Industrially prepared Lactobacillus helveticus strain R0052 was first examined by microarray analysis and then compared to broth-grown strains of R0052 and Lactobacillus rhamnosus strain GG using quantitative realt-time polymerase chain reaction. Results showed host immune activation responses increased following pathogen exposure, which were differentially ameliorated using probiotics depending on both the preparation of probiotics employed and conditions of exposure. These findings provide additional support for the concept that specific probiotic strains serve as a promising option for use in preventing the risk of enteric bacterial infections.
authors: Department of Laboratory Medicine and Pathobiology, University of Toronto, 27 King's College Circle, Toronto, ON M5S 1A1, Canada.
authors: N K
authors: NK
authors: Ho
authors: S P
authors: SP
authors: Hawley
authors: J C
authors: JC
authors: Ossa
authors: O
authors: O
authors: Mathieu
authors: T A
authors: TA
authors: Tompkins
authors: K C
authors: KC
authors: Johnson-Henry
authors: P M
authors: PM
authors: Sherman
mesh_terms: D018938:Caco-2 Cells; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology*; D004926:Escherichia coli / Q000276:immunology*; D020869:Gene Expression Profiling; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D052198:Lactobacillus helveticus / Q000276:immunology*; D052201:Lacticaseibacillus rhamnosus / Q000276:immunology*; D046228:Microarray Analysis; D019936:Probiotics / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D007155:Immunologic Factors