title: Optimized system for cerebral perfusion monitoring in the rat stroke model of intraluminal middle cerebral artery occlusion.
abstract: The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal models of acute ischemic stroke allows to confirm successful arterial occlusion and exclude subarachnoid hemorrhage. Cerebral perfusion monitoring can also be used to study intracranial collateral circulation, which is emerging as a powerful determinant of stroke outcome and a possible therapeutic target. Despite a recognized role of Laser Doppler perfusion monitoring as part of the current guidelines for experimental cerebral ischemia, a number of technical difficulties exist that limit its widespread use. One of the major issues is obtaining a secure and prolonged attachment of a deep-penetration Laser Doppler probe to the animal skull. In this video, we show our optimized system for cerebral perfusion monitoring during transient middle cerebral artery occlusion by intraluminal filament in the rat. We developed in-house a simple method to obtain a custom made holder for twin-fibre (deep-penetration) Laser Doppler probes, which allow multi-site monitoring if needed. A continuous and prolonged monitoring of cerebral perfusion could easily be obtained over the intact skull.
authors: Department of Neuroscience and Biomedical Technologies, University of Milano Bicocca. simone.beretta@unimib.it
authors: Simone
authors: S
authors: Beretta
authors: Matteo
authors: M
authors: Riva
authors: Davide
authors: D
authors: Carone
authors: Elisa
authors: E
authors: Cuccione
authors: Giada
authors: G
authors: Padovano
authors: Virginia
authors: V
authors: Rodriguez Menendez
authors: Giovanni B
authors: GB
authors: Pappadá
authors: Alessandro
authors: A
authors: Versace
authors: Carlo
authors: C
authors: Giussani
authors: Erik P
authors: EP
authors: Sganzerla
authors: Carlo
authors: C
authors: Ferrarese
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000175:diagnosis*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D010477:Perfusion / Q000379:methods; D051381:Rats; D020521:Stroke / Q000175:diagnosis*