title: Elevated phospholipase A2 activities in plasma samples from multiple cancers.
abstract: Only in recent years have phospholipase A2 enzymes (PLA2s) emerged as cancer targets. In this work, we report the first detection of elevated PLA2 activities in plasma from patients with colorectal, lung, pancreatic, and bladder cancers as compared to healthy controls. Independent sets of clinical plasma samples were obtained from two different sites. The first set was from patients with colorectal cancer (CRC; n = 38) and healthy controls (n = 77). The second set was from patients with lung (n = 95), bladder (n = 31), or pancreatic cancers (n = 38), and healthy controls (n = 79). PLA2 activities were analyzed by a validated quantitative fluorescent assay method and subtype PLA2 activities were defined in the presence of selective inhibitors. The natural PLA2 activity, as well as each subtype of PLA2 activity was elevated in each cancer group as compared to healthy controls. PLA2 activities were increased in late stage vs. early stage cases in CRC. PLA2 activities were not influenced by sex, smoking, alcohol consumption, or body-mass index (BMI). Samples from the two independent sites confirmed the results. Plasma PLA2 activities had approximately 70% specificity and sensitivity to detect cancer. The marker and targeting values of PLA2 activity have been suggested.
authors: Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America.
authors: Hui
authors: H
authors: Cai
authors: Elena G
authors: EG
authors: Chiorean
authors: Michael V
authors: MV
authors: Chiorean
authors: Douglas K
authors: DK
authors: Rex
authors: Bruce W
authors: BW
authors: Robb
authors: Noah M
authors: NM
authors: Hahn
authors: Ziyue
authors: Z
authors: Liu
authors: Patrick J
authors: PJ
authors: Loehrer
authors: Marietta L
authors: ML
authors: Harrison
authors: Yan
authors: Y
authors: Xu
mesh_terms: D016022:Case-Control Studies; D004795:Enzyme Stability; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000201:enzymology*; D010190:Pancreatic Neoplasms / Q000097:blood / Q000201:enzymology*; D054467:Phospholipases A2 / Q000097:blood*; D015203:Reproducibility of Results; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000201:enzymology*
chemical_list: D054467:Phospholipases A2