title: Phase-coded volume holographic gratings for spatial-spectral imaging filters.
abstract: We present a design of phase-contrast filters embedded in a three-dimensional pupil to form phase-coded volume holographic gratings (VHGs) for spatial-spectral imaging. The phase-coded VHG improves image contrast and results in strong filtering properties to acquire weak phase structures of an object. In addition, incorporated with in-plane angle multiplexing, the multiplexed phase-coded VHGs enable obtaining weak phase information from multiple depths of an object. We experimentally demonstrate the multiplexed phase-coded VHGs for spatial-spectral imaging to enhance unstained features of spatial-spectral images of human breast cancer cells.
authors: KLA-Tencor Corporation, Milpitas, California 95035, USA.
authors: Se Baek
authors: SB
authors: Oh
authors: Zhan-Qian John
authors: ZQ
authors: Lu
authors: Jui-Chang
authors: JC
authors: Tsai
authors: Hsi-Hsun
authors: HH
authors: Chen
authors: George
authors: G
authors: Barbastathis
authors: Yuan
authors: Y
authors: Luo
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology; D006696:Holography / Q000379:methods*; D006801:Humans; D057054:Molecular Imaging