title: Quantitative approach to skin field cancerization using a nanoencapsulated photodynamic therapy agent: a pilot study.
abstract: BACKGROUND
  This paper introduces a new nanoformulation of 5-aminolevulinic acid (nano-ALA) as well as a novel quantitative approach towards evaluating field cancerization for actinic keratosis and/or skin photodamage. In this pilot study, we evaluated field cancerization using nano-ALA and methyl aminolevulinate (MAL), the latter being commercialized as Metvix(®).
  
  
  RESULTS
  Photodynamic therapy was used for the treatment of patients with selected skin lesions, whereas the fluorescence of the corresponding photosensitizer was used to evaluate the time evolution of field cancerization in a quantitative way. Field cancerization was quantified using newly developed color image segmentation software. Using photodynamic therapy as the precancer skin treatment and the approach introduced herein for evaluation of fluorescent area, we found that the half-life of field cancerization reduction was 43.3 days and 34.3 days for nano-ALA and MAL, respectively. We also found that nano-ALA targeted about 45% more skin lesion areas than MAL. Further, we found the mean reduction in area of skin field cancerization was about 10% greater for nano-ALA than for MAL.
  
  
  CONCLUSIONS
  Although preliminary, our findings indicate that the efficacy of nano-ALA in treating skin field cancerization is higher than that of MAL.
authors: University of Brasília, Institute of Biological Sciences, DF, Brazil ; Foundation for Teaching and Research on Health Sciences, Brasília, DF, Brazil.
authors: Simone K
authors: SK
authors: Passos
authors: Paulo En
authors: PE
authors: de Souza
authors: Priscila Kp
authors: PK
authors: Soares
authors: Danglades Rm
authors: DR
authors: Eid
authors: Fernando L
authors: FL
authors: Primo
authors: Antonio Cláudio
authors: AC
authors: Tedesco
authors: Zulmira Gm
authors: ZG
authors: Lacava
authors: Paulo C
authors: PC
authors: Morais
keywords: 5-aminolevulinic acid; actinic keratosis; field cancerization; nanoemulsion; photodynamic therapy (PDT); skin cancer