title: The identification of a bacterial strain BGI-1 isolated from the intestinal flora of Blattella germanica, and its anti-entomopathogenic fungi activity.
abstract: A bacterial strain BGI-1 was isolated from the gut of German cockroaches (Blattella germanica L.) and was identified as Bacillus subtilis based on 16S rDNA sequence and morphological, physiological, and biochemical characters. The strain BGI-1 inhibited the growth of Beauveria bassiana; the diameter of the inhibition zone exceeded 30 mm. Vesicles were observed in B. bassiana hyphae on the edge of the inhibition zone. Fermentation of BGI-1 reduced the conidial germination rate by 12%. Further studies demonstrated that B. bassiana infections in German cockroaches orally treated with the extracts of BGI-1 fermentation were significantly weakened. Cumulative mortality rate was 49.5% in the treatment group at the 20 d, while that of the control group was 62.3%. The study intends to understand the relationship between the intestinal flora and the cockroach. Those microbes with anti-entomopathogenic fungi activity might contribute to resisting the infection of pathogenic fungi.
authors: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, 88 East Wenhua Road, Jinan 250014, People of Republic of China.
authors: Y H
authors: YH
authors: Huang
authors: X J
authors: XJ
authors: Wang
authors: F
authors: F
authors: Zhang
authors: X B
authors: XB
authors: Huo
authors: R S
authors: RS
authors: Fu
authors: J J
authors: JJ
authors: Liu
authors: W B
authors: WB
authors: Sun
authors: D M
authors: DM
authors: Kang
authors: X
authors: X
authors: Jing
mesh_terms: D000818:Animals; D000898:Antibiosis; D001412:Bacillus subtilis / Q000302:isolation & purification* / Q000502:physiology; D052982:Beauveria / Q000254:growth & development*; D020048:Blattellidae / Q000382:microbiology*; D005285:Fermentation; D008297:Male; D010802:Phylogeny; D013172:Spores, Fungal / Q000502:physiology