title: Chromobacterium violaceum: important insights for virulence and biotechnological potential by exoproteomic studies.
abstract: Chromobacterium violaceum is a beta-proteobacterium with high biotechnological potential, found in tropical environments. This bacterium causes opportunistic infections in both humans and animals, that can spread throughout several tissues, quickly leading to the death of the host. Genomic studies identified potential mechanisms of pathogenicity but no further studies were done to confirm the expression of these systems. In this study 36 unique protein entries were identified in databank from a two-dimensional profile of C. violaceum secreted proteins. Chromobacterium violaceum exoproteomic preliminary studies confirmed the production of proteins identified as virulence factors (such as a collagenase, flagellum proteins, metallopeptidases, and toxins), allowing us to better understand its pathogenicity mechanisms. Biotechnologically interesting proteins (such as chitinase and chitosanase) were also identified among the secreted proteins, as well as proteins involved in the transport and capture of amino acids, carbohydrates, and oxidative stress protection. Overall, the secreted proteins identified provide us important insights on pathogenicity mechanisms, biotechnological potential, and environment adaptation of C. violaceum.
authors: Núcleo de Medicina Tropical, Universidade Federal do Pará, Av. Gen. Deodoro 92, Belém 66055-240, Brazil. alecip@gmail.com
authors: Alessandra
authors: A
authors: Ciprandi
authors: Wanderson Marques
authors: WM
authors: da Silva
authors: Agenor Valadares
authors: AV
authors: Santos
authors: Adriano Monteiro
authors: AM
authors: de Castro Pimenta
authors: Marta Sofia Peixe
authors: MS
authors: Carepo
authors: Maria Paula Cruz
authors: MP
authors: Schneider
authors: Vasco
authors: V
authors: Azevedo
authors: Artur
authors: A
authors: Silva
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D001709:Biotechnology / Q000379:methods*; D002861:Chromobacterium / Q000737:chemistry* / Q000302:isolation & purification / Q000472:pathogenicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D004783:Environmental Microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods*; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D037521:Virulence Factors / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors