title: Mechanical testing of hydrogels in cartilage tissue engineering: beyond the compressive modulus.
abstract: Injuries to articular cartilage result in significant pain to patients and high medical costs. Unfortunately, cartilage repair strategies have been notoriously unreliable and/or complex. Biomaterial-based tissue-engineering strategies offer great promise, including the use of hydrogels to regenerate articular cartilage. Mechanical integrity is arguably the most important functional outcome of engineered cartilage, although mechanical testing of hydrogel-based constructs to date has focused primarily on deformation rather than failure properties. In addition to deformation testing, as the field of cartilage tissue engineering matures, this community will benefit from the addition of mechanical failure testing to outcome analyses, given the crucial clinical importance of the success of engineered constructs. However, there is a tremendous disparity in the methods used to evaluate mechanical failure of hydrogels and articular cartilage. In an effort to bridge the gap in mechanical testing methods of articular cartilage and hydrogels in cartilage regeneration, this review classifies the different toughness measurements for each. The urgency for identifying the common ground between these two disparate fields is high, as mechanical failure is ready to stand alongside stiffness as a functional design requirement. In comparing toughness measurement methods between hydrogels and cartilage, we recommend that the best option for evaluating mechanical failure of hydrogel-based constructs for cartilage tissue engineering may be tensile testing based on the single edge notch test, in part because specimen preparation is more straightforward and a related American Society for Testing and Materials (ASTM) standard can be adopted in a fracture mechanics context.
authors: Chemical and Petroleum Engineering, University of Kansas, Lawrence, Kansas 66045, USA.
authors: Yinghua
authors: Y
authors: Xiao
authors: Elizabeth A
authors: EA
authors: Friis
authors: Stevin H
authors: SH
authors: Gehrke
authors: Michael S
authors: MS
authors: Detamore
mesh_terms: D000818:Animals; D002358:Cartilage, Articular; D006801:Humans; D020100:Hydrogels; D008422:Materials Testing / Q000379:methods* / Q000592:standards; D012038:Regeneration; D044968:Regenerative Medicine / Q000295:instrumentation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods* / Q000592:standards; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels