title: Subcellular control of Rac-GTPase signalling by magnetogenetic manipulation inside living cells.
abstract: Many cell functions rely on the coordinated activity of signalling pathways at a subcellular scale. However, there are few tools capable of probing and perturbing signalling networks with a spatial resolution matching the intracellular dimensions of their activity patterns. Here we present a generic magnetogenetic approach based on the self-assembly of signalling complexes on the surface of functionalized magnetic nanoparticles inside living cells. The nanoparticles act as nanoscopic hot spots that can be displaced by magnetic forces and trigger signal transduction pathways that bring about a cell response. We applied this strategy to Rho-GTPases, a set of molecular switches known to regulate cell morphology via complex spatiotemporal patterns of activity. We demonstrate that the nanoparticle-mediated activation of signalling pathways leads to local remodelling of the actin cytoskeleton and to morphological changes.
authors: Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie, Ecole normale supérieure, Université Pierre et Marie Curie-Paris 6, Paris, France.
authors: F
authors: F
authors: Etoc
authors: D
authors: D
authors: Lisse
authors: Y
authors: Y
authors: Bellaiche
authors: J
authors: J
authors: Piehler
authors: M
authors: M
authors: Coppey
authors: M
authors: M
authors: Dahan
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000378:metabolism; D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D015398:Signal Transduction; D020744:rac GTP-Binding Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000199:Actins; D058185:Magnetite Nanoparticles; D020744:rac GTP-Binding Proteins