title: [Identification of herba rabdosiae serrae from different plant resources by RAPD method].
abstract: OBJECTIVE
  To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis.
  
  
  METHODS
  The mini spin columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the polymer chain reaction (PCR) was done and the results were analysed by electro-pharoses.
  
  
  RESULTS
  12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map.
  
  
  CONCLUSIONS
  RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae.
authors: Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520, China.
authors: Xiao-lu
authors: XL
authors: Mo
authors: Qing-qian
authors: QQ
authors: Zeng
authors: Shan-shan
authors: SS
authors: Huang
authors: Yue-wen
authors: YW
authors: Cai
authors: Yu-sheng
authors: YS
authors: Wang
authors: Zhen
authors: Z
authors: Yan
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D005819:Genetic Markers; D037602:Isodon / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers