title: Ani-survivin DNAzymes inhibit cell proliferation and migration in breast cancer cell line MCF-7.
abstract: Survivin, a new member of the inhibitor of apoptosis protein (IAP) family, both inhibits apoptosis and regulates the cell cycle. It is overexpressed in breast tumor tissues. In this study, we designed two survivin specific DNAzymes (DRz1 and DRz2) targeting survivin mRNA. The results showed that DRz1 could decrease the expression of survivin by nearly 60%. Furthermore, DRz1 significantly inhibited cell proliferation, induced apoptosis and inhibited migration in MCF-7 cells. In addition, down-regulation of survivin expression was associated with increased caspase-3 and -9 activities in MCF-7 cells after 24 h transfection. In our experiments, the efficacy of DRz1 to influence survivin levels and associated effects were better than DRz2. Survivin-DRz1 might have anti-tumorigenic activity and may potentially provide the basis for a novel therapeutic intervention in breast cancer treatment.
authors: Beihua University, Jilin, China.
authors: Min
authors: M
authors: Zhang
authors: Yi-Fu
authors: YF
authors: Sun
authors: Su
authors: S
authors: Luo
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000178:diet therapy* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D021881:DNA, Catalytic / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors*; D061986:MCF-7 Cells; D012333:RNA, Messenger / Q000235:genetics; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D021881:DNA, Catalytic; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin; D053148:Caspase 3; D053453:Caspase 9