title: Halopenitus malekzadehii sp. nov., an extremely halophilic archaeon isolated from a salt lake.
abstract: Strain CC65(T), a novel extremely halophilic archaeon, was isolated from a brine sample of a salt lake in Iran. The novel strain was light yellow-pigmented, non-motile, pleomorphic and required at least 1.7 M NaCl and 0.02 M MgCl₂ for growth. Optimal growth was achieved at 3.5 M NaCl and 0.4 M MgCl₂. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, while it was able to grow over a pH and a temperature range of pH 6.5-9.0 and 30-50 °C, respectively. Analysis of 16S rRNA gene sequence revealed that strain CC65(T) clustered with the sole member of the genus Halopenitus, Halopenitus persicus DC30(T) with a sequence similarity of 98.0%. The polar lipid profile of strain CC65(T) consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. An unidentified glycolipid and two minor phospholipids were also observed. The only quinone present was MK-8(II-H₂). The DNA G+C content of strain CC65(T) was 63.8 mol%. On the basis of the biochemical and physiological characteristics, as well as DNA-DNA hybridization (44% with Halopenitus persicus IBRC 10041(T)), strain CC65(T) is classified as a novel species of the genus Halopenitus, for which the name Halopenitus malekzadehii sp. nov. is proposed. The type strain is CC65(T) ( = IBRC-M 10418(T) =KCTC 4045(T)).
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.|Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors: Mohammad Ali
authors: MA
authors: Amoozegar
authors: Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors: Ali
authors: A
authors: Makhdoumi-Kakhki
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.|Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors: Maliheh
authors: M
authors: Mehrshad
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.
authors: Seyed Abolhassan Shahzadeh
authors: SAS
authors: Fazeli
authors: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors: Antonio
authors: A
authors: Ventosa
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007492:Iran; D060106:Lakes / Q000382:microbiology; D015636:Magnesium Chloride; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012492:Salts; C017082:brine; D015636:Magnesium Chloride; D024482:Vitamin K 2; D012965:Sodium Chloride; C024223:vitamin MK 8