title: [Effect of corticotrophin-releasing hormone on connexin-43 phosphorylation and gap junction intercellular communication in human myometrial smooth muscle cells].
abstract: OBJECTIVE
  To determine the effect of human corticotrophin-releasing hormone (CRH) on the expression of connexin-43 phosphate (P-Cx43) in human myometrial smooth muscle cells (SMCs) and the function of cell gap junction intercellular communication in SMCs.
  
  
  METHODS
  Human non-conceive myometial SMCs were cultured with different concentrations of CRH (0, 5.85, 58.5, 585 and 5850 pmol/L). Western blot was used to test P-Cx43 and Cx43 non-phosphate (NP-Cx43) of protein expression. Cell scratch was used to test cell gap junction intercellular communication opening status in human myometrial SMCs.
  
  
  RESULTS
  Compared with the control group, the expression of P-Cx43 was higher in the CRH groups (P<0.01), and was concentration-dependent. There was no significant difference in NPCx43 between the control group and the CRH groups (P>0.05). The transmission of cell layers in the CRH groups was higher than that in the control group (P<0.01), and as the concentration of CRH increased, the time was concentration-dependent (P<0.01).
  
  
  CONCLUSIONS
  CRH can enhance the expression of P-Cx43 and the function of gap junction intercellular communication in the primary cultured myometrial SMCs.
authors: Department of Obstetrics and Gynecology, Second Xiangya Hospital, Changsha, China.
authors: Yun
authors: Y
authors: Hu
authors: Xin
authors: X
authors: He
authors: Yiling
authors: Y
authors: Ding
mesh_terms: D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D018031:Connexin 43 / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D005260:Female; D017629:Gap Junctions / Q000187:drug effects*; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D009215:Myometrium / Q000166:cytology*; D010766:Phosphorylation / Q000187:drug effects
chemical_list: D018031:Connexin 43; D003346:Corticotropin-Releasing Hormone