title: Effect of fatty acids on the β-oxidation system and thioesterase of Lactococcus lactis subspecies lactis.
abstract: The influence of fatty acids on the β-oxidation system and thioesterase of Lactococcus lactis was investigated in this study. The results showed that fatty acids (C8:0-C16:0) significantly inhibited the growth of Lactococcus lactis, and laurate (C12:0) had the highest bactericidal effects. We detected the maximum activity of β-oxidation at different incubation times (8, 12, and 18h) to be 6.460, 7.751, and 8.203, respectively, and the maximum activity of thioesterase at different incubation times (8, 12, and 18h) to be 19.498, 27.180, and 12.800, respectively. Fatty acids were seen to induce the β-oxidation system and activity of thioesterase; decanoic acid (C10:0) and palmitic acid (C16:0) were also seen to induce the β-oxidation system of Lactococcus lactis, but the induced ability was significantly different. Octanoic acid (C8:0) and palmitic acid (C16:0) were seen to induce thioesterase activity in Lactococcus lactis. When 1mM palmitic acid (C16:0) was added to M17 broth, the activity of thioesterase increased 5-fold after 2min; however, adding octanoic acid (C8:0) changed the activity little. Evidence showed that the ability to induce the β-oxidation system and thioesterase activity was related to the fatty acids' chain lengths.
authors: School of Food Science and Engineering, Harbin Institute of Technology, 202 Haihe Road, Harbin, Heilongjiang 150090, China; College of Food Science, Northeast Agricultural University, 59 Gongbin Road, Harbin, Heilongjiang 150030, China.
authors: Liang
authors: L
authors: Li
authors: School of Food Science and Engineering, Harbin Institute of Technology, 202 Haihe Road, Harbin, Heilongjiang 150090, China. Electronic address: maying@hit.edu.cn.
authors: Ying
authors: Y
authors: Ma
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism*; D000900:Anti-Bacterial Agents; D003652:Decanoic Acids / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology; D007659:Ketones / Q000378:metabolism; D013294:Lactococcus lactis / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development; D007850:Lauric Acids / Q000494:pharmacology; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000494:pharmacology; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism*; D013329:Structure-Activity Relationship; D055549:Volatile Organic Compounds / Q000032:analysis / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003652:Decanoic Acids; D005227:Fatty Acids; D007659:Ketones; D007850:Lauric Acids; D055549:Volatile Organic Compounds; C030358:lauric acid; D019308:Palmitic Acid; C031071:decanoic acid; D015094:3-Hydroxyacyl CoA Dehydrogenases; D010170:Palmitoyl-CoA Hydrolase