title: Probabilistic models for CRISPR spacer content evolution.
abstract: BACKGROUND
  The CRISPR/Cas system is known to act as an adaptive and heritable immune system in Eubacteria and Archaea. Immunity is encoded in an array of spacer sequences. Each spacer can provide specific immunity to invasive elements that carry the same or a similar sequence. Even in closely related strains, spacer content is very dynamic and evolves quickly. Standard models of nucleotide evolution cannot be applied to quantify its rate of change since processes other than single nucleotide changes determine its evolution.
  
  
  METHODS
  We present probabilistic models that are specific for spacer content evolution. They account for the different processes of insertion and deletion. Insertions can be constrained to occur on one end only or are allowed to occur throughout the array. One deletion event can affect one spacer or a whole fragment of adjacent spacers. Parameters of the underlying models are estimated for a pair of arrays by maximum likelihood using explicit ancestor enumeration.
  
  
  RESULTS
  Simulations show that parameters are well estimated on average under the models presented here. There is a bias in the rate estimation when including fragment deletions. The models also estimate times between pairs of strains. But with increasing time, spacer overlap goes to zero, and thus there is an upper bound on the distance that can be estimated. Spacer content similarities are displayed in a distance based phylogeny using the estimated times.We use the presented models to analyze different Yersinia pestis data sets and find that the results among them are largely congruent. The models also capture the variation in diversity of spacers among the data sets. A comparison of spacer-based phylogenies and Cas gene phylogenies shows that they resolve very different time scales for this data set.
  
  
  CONCLUSIONS
  The simulations and data analyses show that the presented models are useful for quantifying spacer content evolution and for displaying spacer content similarities of closely related strains in a phylogeny. This allows for comparisons of different CRISPR arrays or for comparisons between CRISPR arrays and nucleotide substitution rates.
authors: IST Austria (Institute of Science and Technology Austria), Klosterneuburg, Austria. anne.kupczok@ist.ac.at
authors: Anne
authors: A
authors: Kupczok
authors: Jonathan P
authors: JP
authors: Bollback
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000276:immunology; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D003198:Computer Simulation; D019143:Evolution, Molecular; D015233:Models, Statistical; D010802:Phylogeny