title: Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein.
abstract: We review progress in our laboratories toward developing in vivo glucose sensors for diabetes that are based on fluorescence labeling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labeling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for        in vivo sensing. A prototype fiber-optic-based glucose sensor using this technology is being tested.
authors: Diabetes Research Group, King’s College London School of Medicine, Guy’s Hospital, London, United Kingdom. john.pickup@kcl.ac.uk
authors: John C
authors: JC
authors: Pickup
authors: Faaizah
authors: F
authors: Khan
authors: Zheng-Liang
authors: ZL
authors: Zhi
authors: Jonathan
authors: J
authors: Coulter
authors: David J S
authors: DJ
authors: Birch
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation* / Q000379:methods*; D002135:Calcium-Binding Proteins; D003920:Diabetes Mellitus / Q000097:blood*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005947:Glucose; D006801:Humans; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins
chemical_list: D001786:Blood Glucose; D002135:Calcium-Binding Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; C023550:galactose-binding protein; D005947:Glucose