title: A simple and sensitive LC-MS/MS method for quantitation of peimine and its pharmacokinetic in beagle dogs.
abstract: A simple LC-MS/MS method was developed for determination and pharmacokinetic study of peimine in beagle dogs. The chromatographic separation was carried out on a Agilent Zobax SB C18 column with a mobile phase consisting of acetonitrile-10 mmol/L ammonium formate (35:65, v/v) at a flow rate of 0.3 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by MRM via electro spray ionization source with positive mode. The standard curve for peimine was linear (r>0.999) over the concentration range of 1-200 ng/mL with a lower limit of quantification of 1 ng/mL. The intra- and inter-day precision (relative standard deviation) values were not higher than 15% and the accuracy (relative error) was <5% at 3 quality control levels. This simple, fast and highly sensitive method was fully validated and successfully applied to a preclinical pharmacokinetic study of peimine in dogs after oral and intravenous administration.
authors: Department of thoracic surgery, Tumor Hospital, Liaoning Province, China.
authors: W
authors: W
authors: Wang
authors: J
authors: J
authors: Xu
authors: Y Y
authors: YY
authors: Liu
mesh_terms: D000818:Animals; D002595:Cevanes / Q000032:analysis* / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D008297:Male; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002595:Cevanes; C014242:verticine