title: Fluorescent triplex-forming DNA oligonucleotides labeled with a thiazole orange dimer unit.
abstract: Fluorescent probes for the detection of a double-stranded DNA were prepared by labeling a triplex-forming DNA oligonucleotide with a thiazole orange (TO) dimer unit. They belong to ECHO (exciton-controlled hybridization-sensitive fluorescent oligonucleotide) probes which we have previously reported. The excitonic interaction between the two TO molecules was expected to effectively suppress the background fluorescence of the probes. The applicability of the ECHO probes for the detection of double-stranded DNA was confirmed by examining the thermal stability and photophysical and kinetic properties of the DNA triplexes formed by the ECHO probes.
authors: Advanced Science Institute, RIKEN, Saitama, Japan. s-ikeda@riken.jp
authors: Shuji
authors: S
authors: Ikeda
authors: Hiroyuki
authors: H
authors: Yanagisawa
authors: Mizue
authors: M
authors: Yuki
authors: Akimitsu
authors: A
authors: Okamoto
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013816:Thermodynamics
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011804:Quinolines; C051802:triplex DNA; C051000:thiazole orange; D004247:DNA
keywords: DNA; duplex; excitonic interaction; fluorescence; probe; thiazole orange; triplex