title: Changes in Ataxin-10 expression after sciatic nerve crush in adult rats.
abstract: Ataxin-10 is a cytoplasmic protein that belongs to the family of armadillo repeat proteins and the ataxin proteins are ubiquitously expressed in nervous tissue. A loss of Ataxin-10 in primary neuronal cells causes increased apoptosis of cerebellar neurons. Knockdown of ATXN10 with siRNA in HeLa cells results in cytokinesis defects-multinucleation. Because of the essential role of Ataxin-10 in nervous system and cellular cytokinesis, we investigated the spatiotemporal expression of Ataxin-10 in a rat sciatic nerve crush (SNC) model. After never injury, we observed that Ataxin-10 had a significant up-regulation from 3d, peaked at day 5 and then gradually decreased to the normal level at 4 weeks. At its peak expression, Ataxin-10 expressed mainly in Schwann cells and macrophages of the distal sciatic nerve segment from injury, but had few co-localizations in axons. Besides, the peak expression of Ataxin-10 was in parallel with proliferating cell nuclear antigen (PCNA), and Ataxin-10 co-labeled with PCNA. Thus, all of our findings suggested that Ataxin-10 may be involved in the pathophysiology of sciatic nerve after SNC.
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, People's Republic of China.
authors: Xinghai
authors: X
authors: Cheng
authors: Lin
authors: L
authors: Gan
authors: Jing
authors: J
authors: Zhao
authors: Minhao
authors: M
authors: Chen
authors: Yang
authors: Y
authors: Liu
authors: Youhua
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D000067736:Ataxin-10; D049109:Cell Proliferation; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D009409:Nerve Crush; D009419:Nerve Tissue Proteins / Q000235:genetics*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries*
chemical_list: D000067736:Ataxin-10; C496258:Atxn10 protein, rat; D009419:Nerve Tissue Proteins; D018809:Proliferating Cell Nuclear Antigen