title: Non-terminal animal model of post-traumatic osteoarthritis induced by acute joint injury.
abstract: OBJECTIVE
  Develop a non-terminal animal model of acute joint injury that demonstrates clinical and morphological evidence of early post-traumatic osteoarthritis (PTOA).
  
  
  METHODS
  An osteochondral (OC) fragment was created arthroscopically in one metacarpophalangeal (MCP) joint of 11 horses and the contralateral joint was sham operated. Eleven additional horses served as unoperated controls. Every 2 weeks, force plate analysis, flexion response, joint circumference, and synovial effusion scores were recorded. At weeks 0 and 16, radiographs (all horses) and arthroscopic videos (OC injured and sham joints) were graded. At week 16, synovium and cartilage biopsies were taken arthroscopically from OC injured and sham joints for histologic evaluation and the OC fragment was removed.
  
  
  RESULTS
  OC fragments were successfully created and horses were free of clinical lameness after fragment removal. Forelimb gait asymmetry was observed at week 2 (P = 0.0012), while joint circumference (P < 0.0001) and effusion scores (P < 0.0001) were increased in injured limbs compared to baseline from weeks 2 to 16. Positive flexion response of injured limbs was noted at multiple time points. Capsular enthesophytes were seen radiographically in injured limbs. Articular cartilage damage was demonstrated arthroscopically as mild wear-lines and histologically as superficial zone chondrocyte death accompanied by mild proliferation. Synovial hyperemia and fibrosis were present at the site of OC injury.
  
  
  CONCLUSIONS
  Acute OC injury to the MCP joint resulted in clinical, imaging, and histologic changes in cartilage and synovium characteristic of early PTOA. This model will be useful for defining biomarkers of early osteoarthritis and for monitoring response to therapy and surgery.
authors: Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, United States.
authors: M K
authors: MK
authors: Boyce
authors: T N
authors: TN
authors: Trumble
authors: C S
authors: CS
authors: Carlson
authors: D M
authors: DM
authors: Groschen
authors: K A
authors: KA
authors: Merritt
authors: M P
authors: MP
authors: Brown
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D001182:Arthroscopy; D002358:Cartilage, Articular / Q000473:pathology; D005122:Exudates and Transudates; D005260:Female; D005552:Forelimb / Q000473:pathology; D005684:Gait; D006736:Horses; D007596:Joints / Q000293:injuries*; D008297:Male; D010003:Osteoarthritis / Q000000981:diagnostic imaging / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D011859:Radiography; D013583:Synovial Membrane / Q000473:pathology