title: The expression of cytoskeletal proteins in kidney specimens of children with primary focal segmental glomerulosclerosis.
abstract: Several studies have evaluated cytoskeletal proteins as prognostic factors for some types of nephrotic syndrome. However, studies concerning children with FSGS are scarce. This study was done to evaluate the glomerular, tubular, and interstitial expression of vimentin, desmin, and alpha smooth muscle actin (α-SMA) in kidney specimens of children with FSGS. Clinical and histologic data of 31 children with FSGS were reviewed. Thirty one formalin-fixed, paraffin-embedded kidney biopsy sections (3 μm) were selected for immunohistochemical staining. Double immunohistochemistry using a microwave-based two-color staining was applied. The mean age at onset in male and female was 56.3 ± 41.4 and 78.0 ± 60.4 months, respectively. The duration of follow-up was 46.3 ± 56.5 months. Interstitial fibrosis and tubular atrophy were reported in 42% and 54% of the patients, respectively. The latest evaluated mean blood pressure was significantly correlated with the expression of both vimentin and α-SMA in the interstitium (P < 0.05). However, we were not able to demonstrate any cytoskeletal protein expression as an independent predictor for renal survival. Further studies with larger sample size and longer follow-up periods are warranted to investigate the prognostic values of other histopathologic features in pediatrics with FSGS.
authors: Department of Pediatric Nephrology, Isfahan Kidney Diseases Research Center, Child Growth and Developement Research Center, Isfahan, Iran.
authors: A
authors: A
authors: Gheissari
authors: D
authors: D
authors: Taheri
authors: S
authors: S
authors: Mozafarpour
authors: H
authors: H
authors: Beigy
authors: P
authors: P
authors: Samanianpoor
authors: A
authors: A
authors: Merrikhi
authors: Z
authors: Z
authors: Farajzadegan
keywords: Alpha smooth muscle actin; children; desmin; focal segmental glomerulosclerosi; prognostic factor; vimentin