title: Paracellular barrier and tight junction protein expression in the immortalized brain endothelial cell lines bEND.3, bEND.5 and mouse brain endothelial cell 4.
abstract: The blood-brain barrier (BBB) is formed by brain endothelial cells. Many immortalized brain endothelial cell lines have been established; these have been used as in vitro BBB models. The aim of the present study was to assess the paracellular barrier properties of the immortalized mouse brain endothelial cell lines bEND.3, bEND.5 cells, and mouse brain endothelial cell 4 (MBEC4), and those of the primary mouse brain endothelial cells pMBECs. bEND.3 cells showed low permeability to sodium fluorescein and obvious staining of tight junction proteins (claudin-5, occludin and ZO-1) similar to pMBECs; these barrier properties of MBEC4 and bEND.5 cells were low. In addition, bEND.3 cells expressed the highest level of claudin-5 among all cells. These results suggest that bEND.3 cells are a convenient and useful model for evaluating BBB function, especially the paracellular barrier.
authors: Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8–19–1 Nanakuma, Jonan-ku, Fukuoka 814–0180, Japan..
authors: Takuya
authors: T
authors: Watanabe
authors: Shinya
authors: S
authors: Dohgu
authors: Fuyuko
authors: F
authors: Takata
authors: Tsuyoshi
authors: T
authors: Nishioku
authors: Akio
authors: A
authors: Nakashima
authors: Koujiro
authors: K
authors: Futagami
authors: Atsushi
authors: A
authors: Yamauchi
authors: Yasufumi
authors: Y
authors: Kataoka
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply*; D002460:Cell Line; D002470:Cell Survival; D062507:Claudin-5 / Q000032:analysis; D042783:Endothelial Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D062793:Occludin / Q000032:analysis; D062725:Tight Junction Proteins / Q000032:analysis*; D062826:Zonula Occludens-1 Protein / Q000032:analysis
chemical_list: D062507:Claudin-5; C491511:Cldn5 protein, mouse; D062793:Occludin; C568986:Ocln protein, mouse; D062725:Tight Junction Proteins; C569043:Tjp1 protein, mouse; D062826:Zonula Occludens-1 Protein