title: Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection.
abstract: The application of a new rhodamine-based fluorescent probe, RH-NH2 3 and an acyl transfer promoter, PBu3, to Hela cells induced a time-dependent increase in fluorescence in the mitochondria, which was most likely due to acetylation of RH-NH2 3 with activated acetyl-CoA by the artificial reaction promoter in living cells.
authors: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors: Hirokazu
authors: H
authors: Komatsu
authors: Yutaka
authors: Y
authors: Shindo
authors: Shigehiro A
authors: SA
authors: Kawashima
authors: Kenzo
authors: K
authors: Yamatsugu
authors: Kotaro
authors: K
authors: Oka
authors: Motomu
authors: M
authors: Kanai
mesh_terms: D000105:Acetyl Coenzyme A / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects* / Q000378:metabolism*; D057054:Molecular Imaging; D012235:Rhodamines / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D000105:Acetyl Coenzyme A