title: Biocompatibility of BioAggregate and mineral trioxide aggregate on the liver and kidney.
abstract: OBJECTIVE
  To investigate and compare the systemic toxic effect of DiaRoot BioAggregate and grey ProRoot Mineral trioxide aggregate (MTA) on the liver and kidney after 7 and 30 days.
  
  
  METHODS
  Forty-two white albino rats were divided into two main groups. Group (1), considered the control group (n = 18), was further divided into two subgroups. The negative control subgroup (n = 6) received no treatment. The empty tube subgroup (n = 12) received empty sterile Teflon tubes. In Group (2), considered the experimental group (n = 24), the rats were divided equally into two subgroups. One subgroup received MTA, whilst the other received BioAggregate. The materials in the Teflon tubes were implanted subcutaneously in the dorsal side of the rats. Blood samples were taken to investigate the change of kidney and liver functions on day 7 and day 30. The liver and kidney organs were subjected to histopathological examination and calculation of the number of inflammatory cells. Data analysis was performed using one-way anova with post hoc multiple comparisons with the Tukey's test. Student's t-test was used to compare the changes in liver and kidney functions amongst the groups.
  
  
  RESULTS
  On day 7, a significantly more severe inflammatory reaction was observed in both experimental subgroups compared with the control (P < 0.05); the severity decreased after 30 days. The kidney functions were not affected after 7 days but had subsequently increased after 30 days (P < 0.001). Liver functions increased after 7 days and had decreased in the BioAggregate subgroup after 30 days, whilst in the MTA subgroup, a continuous increase in the level of liver function was observed.
  
  
  CONCLUSIONS
  Mineral trioxide aggregate had adverse effects on the liver and kidney that were significantly more severe than BioAggregate but with no permanent damage.
authors: Department of Conservative Dentistry, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia. dr.wafa_endo@hotmail.com
authors: W A
authors: WA
authors: Khalil
authors: N F
authors: NF
authors: Eid
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001672:Biocompatible Materials / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D002126:Calcium Hydroxide / Q000633:toxicity*; D003094:Collagen / Q000032:analysis; D003404:Creatinine / Q000097:blood; D004338:Drug Combinations; D006520:Hepatitis, Animal / Q000097:blood / Q000139:chemically induced; D006882:Hydroxyapatites / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007672:Kidney Cortex / Q000187:drug effects / Q000473:pathology; D007678:Kidney Glomerulus / Q000187:drug effects / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects / Q000473:pathology; D007728:Kupffer Cells / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009393:Nephritis / Q000097:blood / Q000139:chemically induced; D010087:Oxides / Q000633:toxicity*; D011169:Portal Vein / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D040521:Subcutaneous Tissue / Q000601:surgery; D013997:Time Factors; D014508:Urea / Q000097:blood
chemical_list: D017607:Aluminum Compounds; C557009:BioAggregate; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D006882:Hydroxyapatites; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D014508:Urea; D003094:Collagen; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002126:Calcium Hydroxide
keywords: BioAggregate; biocompatibility; effect on liver and kidney; grey MTA