title: A fully attenuated recombinant Salmonid alphavirus becomes pathogenic through a single amino acid change in the E2 glycoprotein.
abstract: A recombinant sleeping disease virus (rSDV) was previously shown to be totally attenuated and provide long-term protection in trout (C. Moriette, M. Leberre, A. Lamoureux, T. L. Lai, M. Brémont, J. Virol. 80:4088-4098, 2006). Sequence comparison of the rSDV to wild-type genomes exhibited a number of nucleotide changes. In the current study, we demonstrate that the virulent phenotype of SDV was essentially associated with two amino acid changes, V8A and M136T, in the E2 glycoprotein, with the V8A change mostly being involved in the acquisition of the virulent phenotype.
authors: Unité de Virologie et Immunologie Moléculaires, INRA, CRJ, Jouy en Josas, France.
authors: Emilie
authors: E
authors: Mérour
authors: Annie
authors: A
authors: Lamoureux
authors: Julie
authors: J
authors: Bernard
authors: Stéphane
authors: S
authors: Biacchesi
authors: Michel
authors: M
authors: Brémont
mesh_terms: D000524:Alphavirus / Q000235:genetics* / Q000472:pathogenicity*; D019943:Amino Acid Substitution; D000818:Animals; D004252:DNA Mutational Analysis; D004195:Disease Models, Animal; D005393:Fish Diseases / Q000821:virology; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D017422:Sequence Analysis, DNA; D016019:Survival Analysis; D014337:Trout; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins; D037521:Virulence Factors