title: Controlled delivery of low-dose bone morphogenetic protein-2 using heparin-conjugated fibrin in the posterolateral lumbar fusion of rabbits.
abstract: The heparin-conjugated fibrin (HCF) system has been developed to deliver bone morphogenetic proteins (BMPs) for a long-term period and thus enhance bone regeneration. In the present study, we tested the effectiveness of the delivery system for spinal fusion with a very low dose of BMP-2. A total of 15 rabbits underwent posterolateral lumbar spine, divided into three groups. The control group received only collagen sponges without BMP-2, another group (BMP-only group) received collagen sponges loaded with BMP-2 (10 μg each side), and the last group (BMP/HCF group) received collagen sponges filled with HCF loaded with BMP-2 (10 μg each side). All animals were euthanized 8 weeks after surgery, and the fusion was assessed by radiographs, manual palpation, computed tomography scan, and mechanical testing. No case in the BMP/HCF group or in the control group achieved solid fusion, while all cases in BMP-only group showed evidence of solid fusion. BMP/HCF group had significantly lower fusion rate and tensile strength than BMP-only group at the dose of 10 μg of BMP-2. The HCF long-term delivery system with the low dose of BMP-2 (10 μg) is ineffective for the induction of lumbar posterolateral fusion in the rabbits.
authors: Department of Orthopaedic Surgery, Dongguk University Ilsan Hospital, Goyang, Korea.
authors: Ki-Hyoung
authors: KH
authors: Koo
authors: Jong-Min
authors: JM
authors: Lee
authors: Jung-Min
authors: JM
authors: Ahn
authors: Byung-Soo
authors: BS
authors: Kim
authors: Wan-Geun
authors: WG
authors: La
authors: Chang-Sung
authors: CS
authors: Kim
authors: Gun-Il
authors: GI
authors: Im
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000737:chemistry; D001861:Bone Regeneration / Q000187:drug effects*; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005337:Fibrin / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology / Q000601:surgery*; D008297:Male; D010173:Palpation; D011817:Rabbits; D013123:Spinal Fusion; D015503:Surgical Sponges; D013718:Tensile Strength; D013997:Time Factors; D014057:Tomography, X-Ray Computed
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005337:Fibrin; D006493:Heparin