title: Mapping and analysis of phosphorylation sites: a quick guide for cell biologists.
abstract: A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.
authors: Department of Cell Biology, Harvard Medical School, Boston, MA 02115-5701, USA.
authors: Noah
authors: N
authors: Dephoure
authors: Kathleen L
authors: KL
authors: Gould
authors: Steven P
authors: SP
authors: Gygi
authors: Douglas R
authors: DR
authors: Kellogg
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D002874:Chromosome Mapping; D030562:Databases, Protein; D010446:Peptide Fragments / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D010750:Phosphoproteins