title: [Alpha-adrenergic regulation of two calcium signal pathways in adipocytes].
abstract: Using selective receptor's agonist and antagonists we show that mouse white fat cells express alpha1A-, alpha2-adrenergic receptors, which activation with noradrenaline is capable of causing calcium responses different by formation mechanism. Adipocyte's calcium responses to alpha1-adrenoreceptor agonists are caused by alpha1A-type adrenoreceptor and suppressed by inhibitors of PLC-dependent pathway. Calcium responses to alpha2-adrenoreceptors agonists are realized only in the presence of more than 200 microM of L-arginine and suppressed by inhibitors of NOS-PKG-RyR pathway. The incubation of cells with L-arginine creates conditions for switching on the signal pathway with participation of eNOS --> NO --> sGC --> cGMP --> PKG --> CD38 --> RyR --> Ca2+ and for switching of the PLC - IP3R-dependent pathway. Adipocyte's calcium response to L-arginine represents a sharp impulse of the big amplitude and is mediated by alpha2-adrenoreceptors. L-arginine activating alpha2-adrenoreceptors and being the substrate of eNOS, realizes two functions in this pathway.
authors: L P
authors: LP
authors: Dolgacheva
authors: E A
authors: EA
authors: Turovskiĭ
authors: M V
authors: MV
authors: Turovskaia
authors: V P
authors: VP
authors: Zinchenko
authors: V V
authors: VV
authors: Dynnik
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D052436:Adipose Tissue, White / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000322:Adrenergic Agonists / Q000494:pharmacology*; D018674:Adrenergic Antagonists / Q000494:pharmacology*; D000818:Animals; D001120:Arginine / Q000378:metabolism / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D061251:Primary Cell Culture; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism*; D013764:Tetrahydronaphthalenes / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: C094643:A 61603; D000322:Adrenergic Agonists; D018674:Adrenergic Antagonists; D004791:Enzyme Inhibitors; D007093:Imidazoles; D018340:Receptors, Adrenergic, alpha-1; D018341:Receptors, Adrenergic, alpha-2; D013764:Tetrahydronaphthalenes; D010656:Phenylephrine; D009569:Nitric Oxide; D001120:Arginine; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse; D010738:Type C Phospholipases; D002118:Calcium; D009638:Norepinephrine