title: Complexity of roles and regulation of the PMK1-MAPK pathway in mycelium development, conidiation and appressorium formation in Magnaporthe oryzae.
abstract: MST50, MST11, MST7, PMK1 and GAS1/GAS2 genes are the important components in the PMK1-MAPK signal transduction pathway in fungi. Mutants with deletion of these five genes of Magnaporthe oryzae, a pathogen of the rice blast, were constructed. A cDNA array containing 4108 unique genes of M. oryzae was developed and used to analyze the gene expression profiles of these mutants against the wild type to dissect the gene expression regulation networks responsible for conidiation and appressorium formation. With this approach, differentially regulated genes by these five components were identified. The vast majority of the regulated genes were mutant-specific, while only a small proportion were in common for all of the mutants, suggesting that each of these genes has its own regulon. Functional groups and expression patterns of the regulated genes showed that (1) gene members in the PMK1-MAPK pathway are associated with multiple signaling pathways; (2) the regulation of PMK1-mediated signaling pathways is very complex and likely involved in other signaling networks; (3) glucose metabolism and signals are required in mycelium development; and (4) appressorium formation likely shares the mechanisms responsible for sexual conjugation and meiosis, which is affected by carbohydrate metabolism.
authors: Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors: Qingchao
authors: Q
authors: Jin
authors: Chanyuan
authors: C
authors: Li
authors: Youzhi
authors: Y
authors: Li
authors: Jinjie
authors: J
authors: Shang
authors: Debao
authors: D
authors: Li
authors: Baoshan
authors: B
authors: Chen
authors: Haitao
authors: H
authors: Dong
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D020082:Magnaporthe / Q000254:growth & development / Q000472:pathogenicity; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D025282:Mycelium / Q000235:genetics / Q000254:growth & development*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D015398:Signal Transduction; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D020928:Mitogen-Activated Protein Kinases; C102726:Pmk1 protein, Magnaporthe grisea; D020929:Mitogen-Activated Protein Kinase Kinases