title: Risk factors for herds to test positive for Mycobacterium avium ssp. paratuberculosis-antibodies with a commercial milk enzyme-linked immunosorbent assay (ELISA) in Ontario and western Canada.
abstract: The objectives of this study were to identify risk factors associated with i) a Mycobacterium avium subsp. paratuberculosis (MAP)-antibody milk enzyme-linked immunosorbent assay (MAP milk ELISA)-positive herd status, and ii) the within-herd MAP milk ELISA-positive prevalence in Canadian dairy herds. This prospective cohort study was conducted between 2005 and 2009 on 226 herds in Ontario and western Canada, which participated in a voluntary risk assessment (RA)-based Johne's disease control program. Two MAP milk ELISA and risk assessments and a previsit survey were available per herd. The overall farm RA scores alone could not be used to predict whether a herd would test positive for MAP antibodies. However, the results of this study indicated that increasing the likelihood of exposing calves to MAP through certain management practices, as assessed with the RA, increased the likelihood of a herd being test-positive for MAP antibodies.
  
  
  
  The objectives of this study were to identify risk factors associated with i) aMycobacterium aviumsubsp.paratuberculosis(MAP)-antibody milk enzyme-linked immunosorbent assay (MAP milk ELISA)-positive herd status, and ii) the within-herd MAP milk ELISA-positive prevalence in Canadian dairy herds. This prospective cohort study was conducted between 2005 and 2009 on 226 herds in Ontario and western Canada, which participated in a voluntary risk assessment (RA)-based Johne’s disease control program. Two MAP milk ELISA and risk assessments and a previsit survey were available per herd. The overall farm RA scores alone could not be used to predict whether a herd would test positive for MAP antibodies. However, the results of this study indicated that increasing the likelihood of exposing calves to MAP through certain management practices, as assessed with the RA, increased the likelihood of a herd being test-positive for MAP antibodies.
authors: University of Minnesota, Department of Veterinary Population Medicine, St. Paul, Minnesota 55108, USA. sorge@umn.edu
authors: Ulrike S
authors: US
authors: Sorge
authors: Kerry
authors: K
authors: Lissemore
authors: Ann
authors: A
authors: Godkin
authors: Jocelyn
authors: J
authors: Jansen
authors: Steven
authors: S
authors: Hendrick
authors: Scott
authors: S
authors: Wells
authors: David F
authors: DF
authors: Kelton
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002170:Canada / Q000453:epidemiology; D002417:Cattle; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D008892:Milk / Q000276:immunology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D009864:Ontario / Q000453:epidemiology; D010283:Paratuberculosis / Q000175:diagnosis / Q000453:epidemiology*; D011446:Prospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial