title: Development and validation of a sensitive LC-MS-MS assay for the quantification of betulonic acid in rat plasma.
abstract: A rapid, sensitive and reliable liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of betulonic acid in rat plasma. The plasma samples were prepared by protein precipitation. Chromatographic separation was achieved on an Agela MG-C18 analytical column (50 × 2.1 mm, 5 µm) using methanol-water-formic acid (80:20:0.1, v/v/v) as the mobile phase with a constant flow rate of 0.6 mL/min. Mass detection was conducted by electrospray ionization in positive ion multiple reaction monitoring mode. The calibration curves were linear over a concentration range of 3.00-3,000 ng/mL for the analyte. The intra-day and inter-day accuracy and precision were within the acceptable limits of ±15% at all concentrations. The method was successfully applied for the estimation of betulonic acid in rat plasma from a preclinical pharmacokinetics study.
authors: 1Department of Pharmacy, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.
authors: Jifeng
authors: J
authors: Gu
authors: Guoqiang
authors: G
authors: Ding
authors: Nianzu
authors: N
authors: Chen
authors: Zhen
authors: Z
authors: Zhang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D031317:Liquidambar / Q000737:chemistry; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000094062:Betulinic Acid
chemical_list: D009828:Oleanolic Acid; C118308:betulonic acid; D000094062:Betulinic Acid