title: A transgenic animal with antiviral properties that might inhibit multiple stages of infection.
abstract: Bombyx mori nucleopolyhedrovirus (BmNPV) is the primary pathogen of silkworms, causing severe economic losses in sericulture. To create antiviral silkworm strains, we constructed a transgenic vector in which the dsRNA for five tandem BmNPV genes was controlled by the BmNPV hr3 enhancer and IE1 promoter. The antivirus gene Bmlipase-1 was driven by B. mori midgut-specific promoter P2. Transgenic strains (SW-H) were generated via embryo microinjection using the practical silkworm strain SW. After infection with a high dose of BmNPV, the survival rates of SW-H and non-transgenic SW were 64% and 13%, respectively. SW-H could be the first transgenic animal that is highly antiviral and that might inhibit the virus at multiple stages of infection.
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
authors: Liang
authors: L
authors: Jiang
authors: Ping
authors: P
authors: Zhao
authors: Tingcai
authors: T
authors: Cheng
authors: Qiang
authors: Q
authors: Sun
authors: Zhengwen
authors: Z
authors: Peng
authors: Yinghui
authors: Y
authors: Dang
authors: Xiangwei
authors: X
authors: Wu
authors: Genhong
authors: G
authors: Wang
authors: Shengkai
authors: S
authors: Jin
authors: Ping
authors: P
authors: Lin
authors: Qingyou
authors: Q
authors: Xia
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000821:virology*; D012831:Bombyx / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000821:virology*; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology; D017924:Nucleopolyhedroviruses / Q000502:physiology*
chemical_list: D019476:Insect Proteins