title: Optimized templates for bottom-up growth of high-performance integrated biomolecular detectors.
abstract: Electrochemical deposition of metals represents an important approach in the bottom-up fabrication of nanostructures and microstructures. We have used this approach to generate high-performance chip-based biosensors using silicon as a platform for the generation of sensor arrays. Here, we explore the applicability of different materials to support the electrodeposition and identify the parameters that are essential for robust sensor growth. We show that inexpensive materials can be used as templates for electrodeposition, and demonstrate that these low-cost sensors exhibit clinically-relevant levels of sensitivity and specificity. In particular, we prove herein that the glass-based sensors successfully detect E. coli in urine, when present at the 100 cfu μL(-1) levels found typically in samples of patients with urinary tract infections.
authors: Department of Chemistry, Faculty of Arts and Sciences, University of Toronto, Toronto, ON, Canada M5S 3M2.
authors: Brian
authors: B
authors: Lam
authors: Richard D
authors: RD
authors: Holmes
authors: Jagotamoy
authors: J
authors: Das
authors: Mahla
authors: M
authors: Poudineh
authors: Andrew
authors: A
authors: Sage
authors: Edward H
authors: EH
authors: Sargent
authors: Shana O
authors: SO
authors: Kelley
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D012329:RNA, Bacterial / Q000652:urine; D012825:Silicon / Q000737:chemistry; D014552:Urinary Tract Infections / Q000382:microbiology / Q000652:urine
chemical_list: D020135:Peptide Nucleic Acids; D012329:RNA, Bacterial; D012825:Silicon