title: Structural plasticity of climbing fibers and the growth-associated protein GAP-43.
abstract: Structural plasticity occurs physiologically or after brain damage to adapt or re-establish proper synaptic connections. This capacity depends on several intrinsic and extrinsic determinants that differ between neuron types. We reviewed the significant endogenous regenerative potential of the neurons of the inferior olive (IO) in the adult rodent brain and the structural remodeling of the terminal arbor of their axons, the climbing fiber (CF), under various experimental conditions, focusing on the growth-associated protein GAP-43. CFs undergo remarkable collateral sprouting in the presence of denervated Purkinje cells (PCs) that are available for new innervation. In addition, severed olivo-cerebellar axons regenerate across the white matter through a graft of embryonic Schwann cells. In contrast, CFs undergo a regressive modification when their target is deleted. In vivo knockdown of GAP-43 in olivary neurons, leads to the atrophy of their CFs and a reduction in the ability to sprout toward surrounding denervated PCs. These findings demonstrate that GAP-43 is essential for promoting denervation-induced sprouting and maintaining normal CF architecture.
authors: Department of Neurobiology, University of Chicago Chicago, IL, USA.
authors: Giorgio
authors: G
authors: Grasselli
authors: Piergiorgio
authors: P
authors: Strata
mesh_terms: D000818:Animals; D001284:Atrophy; D003714:Denervation; D019922:GAP-43 Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D009412:Nerve Fibers / Q000473:pathology / Q000502:physiology*; D009473:Neuronal Plasticity / Q000235:genetics / Q000502:physiology; D009847:Olivary Nucleus / Q000473:pathology / Q000502:physiology*; D011689:Purkinje Cells / Q000473:pathology / Q000502:physiology
chemical_list: D019922:GAP-43 Protein
keywords: GAP-43; atrophy; branching; climbing fiber; sprouting