title: Recurrent pregnancy loss, plasminogen activator inhibitor-1 (-675) 4G/5G polymorphism and antiphospholipid antibodies in Czech women.
abstract: OBJECTIVE
  This study compares the frequencies of plasminogen activator inhibitor-1 (-675) 4G/5G polymorphism and its relationship with eight antiphospholipid antibodies (aPLs) in serum of 157 patients with repeated pregnancy loss (RPL).
  
  
  METHODS
  PAI-1 (-675) 4G/5G polymorphism was determined using standard PCR-RFLP method. Enzyme-linked immunosorbent assay was used for the detection of aPLs against ph-serine, ph-ethanolamine, ph-inositol, ph-DL-glycerol, phosphatidic acid, annexin V, cardiolipin, and beta2-GPI. Allelic frequency and distribution of genotypes were calculated. The prevalence of the risk conferring 4G allele and 4G/4G homozygous genotype in patients and controls was compared, and the correlation between aPLs positivity and PAI-1 4G/4G genotype was tested by chi-square test.
  
  
  RESULTS
  Statistically highly significant correlation between RPL and PAI-1 (-675) 4G/4G genotype was found. No correlation between PAI-1 (-675) 4G/5G polymorphism and the presence of antiphospholipid antibodies in RPL patients was observed.
  
  
  CONCLUSIONS
  PAI-1 (-675) 4G/4G homozygous genotype increases the risk of RPL independently from the aPLs positivity.
authors: Institute of Medical Genetics, Medical Faculty, Charles University and Faculty Hospital, Pilsen, Czech Republic. subrt@fnplzen.cz
authors: Ivan
authors: I
authors: Subrt
authors: Zdenka
authors: Z
authors: Ulcova-Gallova
authors: Monika
authors: M
authors: Cerna
authors: Marketa
authors: M
authors: Hejnalova
authors: Jitka
authors: J
authors: Slovanova
authors: Katarina
authors: K
authors: Bibkova
authors: Zdenka
authors: Z
authors: Micanova
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D000328:Adult; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D018153:Czech Republic; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011110:Polymorphism, Genetic; D011247:Pregnancy; D044465:White People / Q000235:genetics
chemical_list: D017152:Antibodies, Antiphospholipid; D017395:Plasminogen Activator Inhibitor 1