title: Influence of bacteriophage preparations on intracellular killing of bacteria by human phagocytes in vitro.
abstract: Bacteriophages are viruses that infect bacteria. It was shown that bacteriophage therapy is an effective method of combating bacterial infections, including infections caused by antibiotic-resistant bacterial strains. One of the main obstacles to widespread use of phage preparations is limited knowledge regarding the influence of bacteriophages on human organisms. In our study, we evaluated whether application of phage preparations impair bactericidal activities of human phagocytes (granulocytes and monocytes). In our study, we used preparations of phages T2 and T4 specific to Escherichia coli and A3 phage specific to Staphylococcus aureus. We found that bacteriophage preparations do not influence intracellular killing of bacteria by human phagocytes. The effect is irrespective of phage preparation type (lysate, purified phage preparation), phage titer of the preparation, and whether bacteria phagocytosed by phagocyte cells are sensitive or insensitive to phage (bacteriophages homologous and heterologous to bacteria). Although the results of our study are preliminary, they support previous data indicating safety of therapeutic application of phages.
authors: Laboratory of Bacteriophages, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. aneta.kurzepa@up.wroc.pl
authors: Aneta
authors: A
authors: Kurzepa-Skaradzinska
authors: Marzanna
authors: M
authors: Lusiak-Szelachowska
authors: Grzegorz
authors: G
authors: Skaradzinski
authors: Ewa
authors: E
authors: Jonczyk-Matysiak
authors: Beata
authors: B
authors: Weber-Dabrowska
authors: Maciej
authors: M
authors: Zaczek
authors: Tomasz
authors: T
authors: Maj
authors: Anna
authors: A
authors: Slawek
authors: Waldemar
authors: W
authors: Rymowicz
authors: Marlena
authors: M
authors: Klak
authors: Ryszard
authors: R
authors: Miedzybrodzki
authors: Andrzej
authors: A
authors: Gorski
mesh_terms: D017122:Bacteriophage T4 / Q000502:physiology*; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D050296:Microbial Viability; D010586:Phagocytes / Q000276:immunology*; D013204:Staphylococcus Phages / Q000502:physiology*; D013211:Staphylococcus aureus / Q000276:immunology*