title: Isoflavone determination in spontaneous legumes identified by DNA barcodes.
abstract: Isoflavones have been associated with several health protective effects. In this work spontaneous legume plants were screened as putative sources of dietary isoflavones. A molecular identification of the collected species was performed throughout DNA barcoding using ITS, rbcL, rpoC1 and matK sequences. The use of a multi-locus barcoding system complemented with basic morphological information allowed the unequivocal identification at the species level of 90% of the samples. The determination of isoflavone content was performed by high-performance liquid chromatography with diode-array detection. Total average contents in the studied species were significantly different, Ononis natrix and Cytisus scoparius possessing the highest total isoflavones content (396 and 273 mg kg(-1), respectively) and Lotus creticus, the lowest (20 mg kg(-1)). The correlation of total isoflavone content with the phylogeny of this set of plants as determined by the rpoC1 sequences was evaluated for the first time.
authors: REQUIMTE, Laboratory of Food and Water Science, Faculty of Pharmacy of University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. sara.cunha@ff.up.pt
authors: Sara C
authors: SC
authors: Cunha
authors: Miguel A
authors: MA
authors: Faria
authors: Tiago
authors: T
authors: Sousa
authors: Eugénia
authors: E
authors: Nunes
mesh_terms: D058893:DNA Barcoding, Taxonomic / Q000379:methods; D018744:DNA, Plant / Q000235:genetics; D007887:Fabaceae / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D007529:Isoflavones / Q000032:analysis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D014675:Vegetables / Q000737:chemistry* / Q000145:classification* / Q000235:genetics
chemical_list: D018744:DNA, Plant; D007529:Isoflavones