title: Nanostructured and thermoresponsive recombinant biopolymer-based microcapsules for the delivery of active molecules.
abstract: Multilayer capsules conceived at the nano- and microscales are receiving increasing interest due to their potential role as carriers of biomolecules for drug delivery and tissue engineering. Herein we report the construction of microcapsules by the sequential adsorption of chitosan and a biomimetic elastin-like recombinamer into nanostructured layers on inorganic microparticle templates. The release profile of bovine serum albumin, which was studied at 25 and 37 °C, shows higher retention and Fickian diffusion at physiological temperature. The self-assembled multilayers act as a barrier and allowed for sustained release over 14 days. The capsules studied are non-cytotoxic towards L929 cells, thereby suggesting multiple applications in the fields of biotechnology and bioengineering, where high control of the delivery of therapeutics and growth/differentiation factors is required.
  
  
  BACKGROUND
  In this paper, the construction of microcapsules by sequential adsorption of chitosan and a biomimetic, elastin-like recombinamer into nanostructured layers on inorganic microparticle templates is reported. The layers demonstrated sustained drug release over 14 days. These microcapsules are non-cytotoxic toward L929 cells, suggesting multiple applications where high control of drug or growth factor delivery is required.
authors: 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal; ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors: Rui R
authors: RR
authors: Costa
authors: Catarina A
authors: CA
authors: Custódio
authors: Francisco J
authors: FJ
authors: Arias
authors: José C
authors: JC
authors: Rodríguez-Cabello
authors: João F
authors: JF
authors: Mano
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D002119:Calcium Carbonate / Q000737:chemistry; D002214:Capsules / Q000737:chemistry*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival; D016503:Drug Delivery Systems / Q000379:methods*; D004549:Elastin / Q000737:chemistry; D007700:Kinetics; D051379:Mice; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011994:Recombinant Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013696:Temperature
chemical_list: D001704:Biopolymers; D002214:Capsules; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine; D004549:Elastin; D002119:Calcium Carbonate
keywords: Biomaterials; Drug delivery; Layer-by-layer; Protein encapsulation; Tissue engineering