title: [Triiodothyronine does not increase osteogenic differentiation reduced by age in bone marrow mesenchymal stem cells of female rats].
abstract: OBJECTIVE
  To examine if triiodothyronine (T3) increases osteogenic differentiation in bone marrow mesenchymal stem cells (BMMSCs) of adult rats compared with young rats.
  
  
  METHODS
  BMMSCs were cultured in osteogenic medium and distributed into six groups: 1) BMMSCs of young rats; 2) BMMSCs of adult rats; 3, 4, 5 and 6) BMMSCs of adult rats with T3 (0.01, 1, 100 to 1000 nM). We analyzed alkaline phosphatase activity, dimethylthiazol (MTT) conversion, and collagen synthesis at 7, 14, and 21 days, and percentage of cells per field and number of mineralized nodules at 21 days of differentiation.
  
  
  RESULTS
  T3 reduced MTT conversion, alkaline phosphatase activity, collagen synthesis, and the synthesis of mineralizalized nodules in at least one of the doses and periods studied (p < 0.05). Values were lower when compared with young and adult rats BMMSCs (p < 0.05) without T3.
  
  
  CONCLUSIONS
  T3 has a negative effect on the factors involved in osteogenic differentiation of BMMSC from adult rats.
authors: Núcleo de Células-tronco e Terapia Celular, Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brasil.
authors: Jankerle Neves
authors: JN
authors: Boeloni
authors: Natalia Melo
authors: NM
authors: Ocarino
authors: Alfredo Miranda
authors: AM
authors: Goes
authors: Rogéria
authors: R
authors: Serakides
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D023421:Models, Animal; D010012:Osteogenesis / Q000187:drug effects*; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D013778:Tetrazolium Salts; D013844:Thiazoles; D014284:Triiodothyronine; D003094:Collagen; D000469:Alkaline Phosphatase; C022616:thiazolyl blue