title: Screening method for the detection of methamphetamine in hair using fluorescence polarization immunoassay.
abstract: A hair screening method has been developed for the detection of methamphetamine using an immunoassay analyzer (AxSYM) with a fluorescence polarization immunoassay (FPIA) technique. The method consisted of washing, cutting and digesting a hair sample (5 mg) with an enzymatic digestion solution. The digested hair sample was centrifuged, and then an aliquot of the supernatant was used to conduct the screening. The results obtained from FPIA, in most cases, showed concentrations above 70.0 ng/mL of methamphetamine for hair samples that contained 0.5 ng/mg of methamphetamine, determined by gas chromatography-mass spectrometry (GC-MS). The percent sensitivity, defined as the true positive rate of screened and confirmed results, and the percent specificity, defined as the true negative rate of screened and confirmed results, of the FPIA screening method were 100.0 and 96.7% (false positive rate of 3.3%), respectively, when the threshold level for FPIA analysis was set at 70.0 ng/mL (n = 60).The correlation coefficient (r) for the linear relationship between FPIA and GC-MS results was 0.91 in real hair samples. The recommended amount of hair sample was found to be 5.0 mg for FPIA screening analysis when the concentration of methamphetamine in hair samples determined by GC-MS was found to be more than 0.5 ng/mg. The method developed in this study was reliable and effective for the screening of methamphetamine in routine hair analysis.
authors: Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecution Service, Seocho-Gu, Seoul 137-730, Republic of Korea. saturn-jjc@spo.go.kr
authors: Jae Chul
authors: JC
authors: Cheong
authors: SungIll
authors: S
authors: Suh
authors: Beom Jun
authors: BJ
authors: Ko
authors: Jae Il
authors: JI
authors: Lee
authors: Jin Young
authors: JY
authors: Kim
authors: Yong Jun
authors: YJ
authors: Suh
authors: Moon Kyo
authors: MK
authors: In
mesh_terms: D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis; D008694:Methamphetamine / Q000032:analysis*; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection
chemical_list: D013287:Illicit Drugs; D008694:Methamphetamine