title: Amperometric biosensor based on reductive H2O2 detection using pentacyanoferrate-bound polymer for creatinine determination.
abstract: Pentacyanoferrate-bound poly(1-vinylimidazole) (PVI[Fe(CN)5]) was selected as a mediator for amperometric creatinine determination based on the reductive H2O2 detection. Creatinine amidohydrolase (CNH), creatine amidohydrolase (CRH), sarcosine oxidase (SOD), peroxidase (POD), and PVI[Fe(CN)5] were crosslinked with poly(ethylene glycol) diglycidyl ether (PEGDGE) on a glassy carbon (GC) electrode for a creatinine biosensor fabrication. Reduction current was monitored at -0.1V in the presence of creatinine and O2. It is revealed that PVI[Fe(CN)5] is suitable as a mediator for a bioelectrocatalytic reaction of POD, since PVI[Fe(CN)5] neither reacts with reactants nor works as an electron acceptor of SOD. The amounts of PVI[Fe(CN)5], PEGDGE, and enzymes were optimized toward creatinine detection. Nafion as a protecting film successfully prevented the enzyme layer from interferences. The detection limit and linear range in creatinine determination were 12μM and 12-500μM (R(2)=0.993), respectively, and the sensitivity was 11mAcm(-2)M(-1), which is applicable for urine creatinine tests. The results of the creatinine determination for four urine samples measured with this proposed method were compared with Jaffe method, and a good correlation was obtained between the results.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University Sakyo, Kyoto 606-8502, Japan.
authors: Chi-Hua
authors: CH
authors: Nieh
authors: Seiya
authors: S
authors: Tsujimura
authors: Osamu
authors: O
authors: Shirai
authors: Kenji
authors: K
authors: Kano
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000295:instrumentation; D003404:Creatinine / Q000032:analysis*; D004566:Electrodes; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D007093:Imidazoles / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism; D011145:Polyvinyls / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D007093:Imidazoles; D011145:Polyvinyls; C121008:pentacyanoferrate (III); C524090:poly(1-vinylimidazole); D003404:Creatinine; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D009195:Peroxidase