title: Expression of proteins in serum, synovial fluid, synovial membrane, and articular cartilage samples obtained from dogs with stifle joint osteoarthritis secondary to cranial cruciate ligament disease and dogs without stifle joint arthritis.
abstract: OBJECTIVE
  To identify proteins with differential expression between healthy dogs and dogs with stifle joint osteoarthritis secondary to cranial cruciate ligament (CCL) disease.
  
  
  METHODS
  Serum and synovial fluid samples obtained from dogs with stifle joint osteoarthritis before (n = 10) and after (8) surgery and control dogs without osteoarthritis (9) and archived synovial membrane and articular cartilage samples obtained from dogs with stifle joint osteoarthritis (5) and dogs without arthritis (5).
  
  
  METHODS
  Serum and synovial fluid samples were analyzed via liquid chromatography-tandem mass spectrometry; results were compared against a nonredundant protein database. Expression of complement component 3 in archived tissue samples was determined via immunohistochemical methods.
  
  
  RESULTS
  No proteins had significantly different expression between serum samples of control dogs versus those of dogs with stifle joint osteoarthritis. Eleven proteins (complement component 3 precursor, complement factor I precursor, apolipoprotein B-100 precursor, serum paraoxonase and arylesterase 1, zinc-alpha-2-glycoprotein precursor, serum amyloid A, transthyretin precursor, retinol-binding protein 4 precursor, alpha-2-macroglobulin precursor, angiotensinogen precursor, and fibronectin 1 isoform 1 preproprotein) had significantly different expression (> 2.0-fold) between synovial fluid samples obtained before surgery from dogs with stifle joint osteoarthritis versus those obtained from control dogs. Complement component 3 was strongly expressed in all (5/5) synovial membrane samples of dogs with stifle joint osteoarthritis and weakly expressed in 3 of 5 synovial membrane samples of dogs without stifle joint arthritis.
  
  
  CONCLUSIONS
  Findings suggested that the complement system and proteins involved in lipid and cholesterol metabolism may have a role in stifle joint osteoarthritis, CCL disease, or both.
authors: Comparative Orthopaedic Laboratory, University of Missouri, Columbia, MO 65211, USA.
authors: Bridget C
authors: BC
authors: Garner
authors: Keiichi
authors: K
authors: Kuroki
authors: Aaron M
authors: AM
authors: Stoker
authors: Cristi R
authors: CR
authors: Cook
authors: James L
authors: JL
authors: Cook
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000378:metabolism*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007592:Joint Diseases / Q000097:blood / Q000378:metabolism* / Q000662:veterinary*; D008297:Male; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D040901:Proteomics / Q000379:methods; D013583:Synovial Membrane / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000662:veterinary