title: NIR-emitting quantum dot-encoded microbeads through membrane emulsification for multiplexed immunoassays.
abstract: NIR-emitting CdSeTe/CdS/ZnS core/shell/shell QD-encoded microbeads are combined with common flow cytometry with one laser for multiplexed detection of hepatitis B virus (HBV). A facile one-pot synthetic route is developed to prepare CdSeTe/CdS/ZnS core/shell/shell QDs with high photoluminescence quantum yield and excellent stability in liquid paraffin, and a Shirasu porous glass (SPG) membrane emulsification technique is applied to incorporate the QDs into polystyrene-maleic anhydride (PSMA) microbeads to obtain highly fluorescent QD-encoded microbeads. The relatively wide NIR photoluminescence full width half maximum of the CdSeTe/CdS/ZnS QDs is used to develop a 'single wavelength' encoding method to obtain different optical codes by changing the wavelengh and emission intensity of the QDs incorporated into the microbeads. Moreover, a detection platform combining NIR-emitting CdSeTe/CdS/ZnS QD-encoded microbeads and Beckman Coulter FC 500 flow cytometry with one laser of 488 nm is successfully used to conduct a 2-plex hybridization assay for hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and a 3-plex hybridization assay for hepatitis B surface antibody (HBsAb), hepatitis B e antibody (HBeAb), and hepatitis B core antibody (HBcAb), which suggests the promising application of NIR QD-encoded microbeads for multiplex immunoassays.
authors: State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors: Xiebing
authors: X
authors: Wang
authors: Gang
authors: G
authors: Wang
authors: Wanwan
authors: W
authors: Li
authors: Bingxia
authors: B
authors: Zhao
authors: Bin
authors: B
authors: Xing
authors: Yuankui
authors: Y
authors: Leng
authors: Hongjing
authors: H
authors: Dou
authors: Kang
authors: K
authors: Sun
authors: Lisong
authors: L
authors: Shen
authors: Xiangliang
authors: X
authors: Yuan
authors: Jiyu
authors: J
authors: Li
authors: Kun
authors: K
authors: Sun
authors: Junsong
authors: J
authors: Han
authors: Huasheng
authors: H
authors: Xiao
authors: Yue
authors: Y
authors: Li
authors: Peng
authors: P
authors: Huang
authors: Xiaoyuan
authors: X
authors: Chen
mesh_terms: D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D045663:Quantum Dots
keywords: SPG membrane emulsification; immunoassays; microbeads; multiplexed detection; quantum dots