title: Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature.
abstract: OBJECTIVE
  The aim of this work was to clarify the mechanism of monounsaturated fatty acid (MUFA) synthesis in Bradyrhizobium TAL1000 and the effect of high temperature on this process.
  
  
  RESULTS
  Bradyrhizobium TAL1000 was exposed to a high growth temperature and heat shock, and fatty acid composition and synthesis were tested. To determine the presence of a possible desaturase, a gene was identify and overexpressed in Escherichia coli. The desaturase expression was detected by RT-PCR and Western blotting. In B. TAL1000, an aerobic mechanism for MUFA synthesis was detected. Desaturation was decreased by high growth temperature and by heat shock. Two hours of exposure to 37°C were required for the change in MUFA levels. A potential ∆9 desaturase gene was identified and successfully expressed in E. coli. A high growth temperature and not heat shock reduced transcript and protein desaturase levels in rhizobial strain.
  
  
  CONCLUSIONS
  In B. TAL1000, the anaerobic MUFA biosynthetic pathway is supplemented by an aerobic mechanism mediated by desaturase and is down-regulated by temperature to maintain membrane fluidity under stressful conditions.
  
  
  CONCLUSIONS
  This knowledge will be useful for developing strategies to improve a sustainable practice of this bacterium under stress and to enhance the bioprocess for the inoculants' manufacture.
authors: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors: N S
authors: NS
authors: Paulucci
authors: D B
authors: DB
authors: Medeot
authors: M
authors: M
authors: Woelke
authors: M S
authors: MS
authors: Dardanelli
authors: M G
authors: MG
authors: de Lema
mesh_terms: D000332:Aerobiosis; D000595:Amino Acid Sequence; D010367:Arachis / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D020369:Bradyrhizobium / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics; D005227:Fatty Acids / Q000096:biosynthesis*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D008560:Membrane Fluidity; D008969:Molecular Sequence Data; D055170:Plant Root Nodulation; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D044943:Fatty Acid Desaturases