title: Determination of disk diffusion and MIC quality control guidelines for GSK2251052: a novel boron-containing antibacterial.
abstract: GSK2251052 is a boron-containing antimicrobial agent in clinical development for the treatment of serious Gram-negative bacterial infections. These GSK2251052 quality control (QC) studies were performed to establish ranges for control strains (broth microdilution [BMD] MIC and disk diffusion zones) as follows: Pseudomonas aeruginosa ATCC 27853 (2-8 μg/mL and 15-24 mm), Escherichia coli ATCC 25922 (0.5-2 μg/mL and 23-30 mm), Haemophilus influenzae ATCC 49247 (0.25-1 μg/mL and 22-31 mm), Streptococcus pneumoniae ATCC 49619 (0.25-1 μg/mL and 19-28 mm), Bacteroides fragilis ATCC 25285 (1-4 μg/mL [BMD] and 1-4 μg/mL [agar dilution]), and Bacteroides thetaiotaomicron ATCC 29741 (1-8 μg/mL [BMD] and 2-8 μg/mL [agar dilution]). These ranges, approved by Clinical and Laboratory Standards Institute, will be crucial in accurately evaluating GSK2251052 in vitro potency.
authors: JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317, USA.
authors: James E
authors: JE
authors: Ross
authors: Nicole
authors: N
authors: Scangarella-Oman
authors: Ronald N
authors: RN
authors: Jones
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards*; D011786:Quality Control
chemical_list: D000900:Anti-Bacterial Agents