title: Atomic force microscopy of chronic lymphatic leukaemia cells activation induced by Staphylococcus aureus.
abstract: Activation of lymphatic cells is associated with changes in morphology, ultrastructure and adhesion force. We have investigated the activation efficiency of Staphylococcus aureus (SAC) on B-cell chronic lymphatic leukaemia (B-CLL) cells using atomic force microscopy (AFM), and found changes in the above properties. Cell viability and proliferation were measured using Cell Counting Kit-8 (CCK-8) and enzyme-linked immunosorbent assay (ELISA). AFM clearly showed that the volume and nuclear-cytoplasm ratio of cells increased significantly with activated time. It also showed that pseudopodia and immunological synapses began to appear at 24 h. In the activation process, nano-structures of the cell surface became aggregated, and adhesion increased. In conclusion, the results indicate a close relationship between membrane reconstruction and multiplication process of B-CLL cells.
authors: Department of Chemistry, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China.
authors: Shisong
authors: S
authors: Dong
authors: Qiulan
authors: Q
authors: Wang
authors: Shaoyang
authors: S
authors: Sun
authors: Yuanwei
authors: Y
authors: Liang
authors: Jinhuan
authors: J
authors: Jiang
authors: Li
authors: L
authors: Liu
authors: Anbin
authors: A
authors: Hu
authors: Jiye
authors: J
authors: Cai
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D002462:Cell Membrane / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D016207:Cytokines / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology; D008213:Lymphocyte Activation; D018625:Microscopy, Atomic Force; D013211:Staphylococcus aureus / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D016207:Cytokines
keywords: B-cell chronic lymphatic leukaemia; Staphylococcus aureus; atomic force microscopy; proliferation