title: LGALS3BP regulates centriole biogenesis and centrosome hypertrophy in cancer cells.
abstract: Centrosome morphology and number are frequently deregulated in cancer cells. Here, to identify factors that are functionally relevant for centrosome abnormalities in cancer cells, we established a protein-interaction network around 23 centrosomal and cell-cycle regulatory proteins, selecting the interacting proteins that are deregulated in cancer for further studies. One of these components, LGALS3BP, is a centriole- and basal body-associated protein with a dual role, triggering centrosome hypertrophy when overexpressed and causing accumulation of centriolar substructures when downregulated. The cancer cell line SK-BR-3 that overexpresses LGALS3BP exhibits hypertrophic centrosomes, whereas in seminoma tissues with low expression of LGALS3BP, supernumerary centriole-like structures are present. Centrosome hypertrophy is reversed by depleting LGALS3BP in cells endogenously overexpressing this protein, supporting a direct role in centrosome aberration. We propose that LGALS3BP suppresses assembly of centriolar substructures, and when depleted, causes accumulation of centriolar complexes comprising CPAP, acetylated tubulin and centrin.
authors: Department of Vertebrate Genomics, Max-Planck Institute for Molecular Genetics, 14195 Berlin, Germany.
authors: Marie-Laure
authors: ML
authors: Fogeron
authors: Hannah
authors: H
authors: Müller
authors: Sophia
authors: S
authors: Schade
authors: Felix
authors: F
authors: Dreher
authors: Verena
authors: V
authors: Lehmann
authors: Anne
authors: A
authors: Kühnel
authors: Anne-Kathrin
authors: AK
authors: Scholz
authors: Karl
authors: K
authors: Kashofer
authors: Alexandra
authors: A
authors: Zerck
authors: Beatrix
authors: B
authors: Fauler
authors: Rudi
authors: R
authors: Lurz
authors: Ralf
authors: R
authors: Herwig
authors: Kurt
authors: K
authors: Zatloukal
authors: Hans
authors: H
authors: Lehrach
authors: Johan
authors: J
authors: Gobom
authors: Eckhard
authors: E
authors: Nordhoff
authors: Bodo M H
authors: BM
authors: Lange
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002502:Centrioles / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D002846:Chromatography, Affinity; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D006984:Hypertrophy; D008297:Male; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D060066:Protein Interaction Maps; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018239:Seminoma / Q000235:genetics / Q000473:pathology; D008941:Spindle Apparatus / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C081856:LGALS3BP protein, human; C492608:Lgals3bp protein, rat; D034741:RNA, Small Interfering; C490219:PLK4 protein, human; D017346:Protein Serine-Threonine Kinases