title: Molecular biological identification of malassezia yeasts using pyrosequencing.
abstract: BACKGROUND
  A Pyrosequencing assay has been used in identification of fungal species such as Candida or Aspergillus and diagnosis of pathogenic bacteria such as Helicobacter pylori but there has been no report on successful isolation and identification of Malassezia yeasts using the pyrosequencing method.
  
  
  OBJECTIVE
  Examine the applicability and plausibility of the pyrosequencing method in identification of the Malassezia species.
  
  
  METHODS
  At internal transcribed spacer (ITS) sites 1 and 2, three primers were developed using Pyrosequencing Assay Design Software (Biotage AB). Pyrosequencing was performed on 11 standard strains and 83 genomic DNA samples obtained from 66 healthy controls aged from 1 to 80.
  
  
  RESULTS
  The eleven Malassezia standard species and 83 genomic DNA samples were successfully identified using the pyrosequencing assay.
  
  
  CONCLUSIONS
  The pyrosequencing method is a new tool for analysis of Malassezia yeasts, and its precision and rapidity suggests its clinical applicability.
authors: Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
authors: Ji Young
authors: JY
authors: Kim
authors: Hyung Jin
authors: HJ
authors: Hahn
authors: Yong Beom
authors: YB
authors: Choe
authors: Yang Won
authors: YW
authors: Lee
authors: Kyu Joong
authors: KJ
authors: Ahn
authors: Kee Chan
authors: KC
authors: Moon
keywords: Malassezia yeasts; Pyrosequencing assay