title: Anti-inflammatory properties of potato glycoalkaloids in stimulated Jurkat and Raw 264.7 mouse macrophages.
abstract: OBJECTIVE
  The potato glycoalkaloids, α-chaconine, α-solanine and solanidine, along with potato peel extracts were investigated for potential anti-inflammatory effects in vitro. Their potential to reduce two biomarkers of inflammation, cytokine and nitric oxide (NO) productions, were assessed in the stimulated Jurkat and macrophage models, respectively.
  
  
  METHODS
  Cytokine and nitric oxide productions were stimulated in Jurkat and Raw 264.7 macrophages with Concanavalin A (Con A; 25 μg/ml) and lipopolysaccaride (LPS; 1 μg/ml), respectively. Selective concentrations of glycoalkaloids and potato peel extracts were added simultaneously with Con A or LPS for 24h to investigate their potential to reduce inflammatory activity.
  
  
  RESULTS
  α-Chaconine and solanidine significantly reduced interleukin-2 (IL-2) and interleukin-8 (IL-8) productions in Con A-induced Jurkat cells. The potato peel extracts did not influence cytokine production. In LPS-stimulated Raw macrophages, α-solanine, solanidine and two potato peel extracts significantly reduced induced NO production.
  
  
  CONCLUSIONS
  Our findings suggest that sub-cytotoxic concentrations of potato glycoalkaloids and potato peel extracts possess anti-inflammatory effects in vitro and with further investigation may be useful in the prevention of anti-inflammatory diseases.
authors: School of Food and Nutritional Sciences, University College Cork, Cork, Ireland.
authors: Olivia M
authors: OM
authors: Kenny
authors: Catherine M
authors: CM
authors: McCarthy
authors: Nigel P
authors: NP
authors: Brunton
authors: Mohammad B
authors: MB
authors: Hossain
authors: Dilip K
authors: DK
authors: Rai
authors: Stuart G
authors: SG
authors: Collins
authors: Peter W
authors: PW
authors: Jones
authors: Anita R
authors: AR
authors: Maguire
authors: Nora M
authors: NM
authors: O'Brien
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D003208:Concanavalin A / Q000276:immunology; D004144:Diosgenin / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology; D019169:Jurkat Cells / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology*; D012992:Solanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D007376:Interleukin-2; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010936:Plant Extracts; C514919:alpha-solanine; D003208:Concanavalin A; D012992:Solanine; C011860:alpha-chaconine; D009569:Nitric Oxide; D004144:Diosgenin; C013255:solanidine