title: Fengycin C produced by Bacillus subtilis EA-CB0015.
abstract: Bacillus subtilis EA-CB0015 was isolated from the phyllosphere of a banana plant and tested for its potential to produce bioactive compounds against Mycosphaerella fijiensis. Using a dual plate culture technique the cell-free supernatant of B. subtilis EA-CB0015 produced inhibition values of 89 ± 1%. The active compounds were purified by solid-phase extraction and HPLC, and their primary structures determined using mass spectrometry and amino acid analysis. A new fengycin isoform, fengycin C, with the amino acid sequence Glu-Orn-Tyr-Thr-Glu-Val-Pro-Gln-Thr-Ile was isolated. The peptidic moiety differs from fengycin B at position 9 and from fengycin A at positions 6 and 9. The β-hydroxy fatty acyl chain is connected to the N-terminal of the decapeptide and can be saturated or unsaturated, ranging from 14 to 18 carbons. The C-terminal residue of the peptidic moiety is linked to the tyrosine residue at position 3, forming the branching point of the acyl peptide and the eight-membered cyclic lactone.
authors: Grupo GIPAB, Departamento de Ingeniería de Procesos, Universidad EAFIT, Medellín, Colombia. vvilleg2@eafit.edu.co
authors: Valeska
authors: V
authors: Villegas-Escobar
authors: Isabel
authors: I
authors: Ceballos
authors: John J
authors: JJ
authors: Mira
authors: Luz Edith
authors: LE
authors: Argel
authors: Sergio
authors: S
authors: Orduz Peralta
authors: Magally
authors: M
authors: Romero-Tabarez
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects; D001412:Bacillus subtilis / Q000737:chemistry*; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D028521:Musa / Q000382:microbiology; D010456:Peptides, Cyclic / Q000737:chemistry; D020033:Protein Isoforms; D014443:Tyrosine / Q000737:chemistry / Q000235:genetics
chemical_list: D000935:Antifungal Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic; D020033:Protein Isoforms; C049972:fengycin; D014443:Tyrosine