title: A new generation of food-borne pathogen detection based on ribosomal RNA.
abstract: Listeria and Salmonella detection assays for food and environmental surfaces that target ribosomal RNA (rRNA) have been developed. The large number of rRNA molecules in bacteria enabled the development of molecular assays that use enrichment times as short as 12 hours for Salmonella and 24 hours for Listeria. These assays run on a fully automated molecular pathogen detection system, which provides walk-away capability and produces 300 assay results in eight hours.
authors: Roka Bioscience Inc., San Diego, CA, USA. klivezey@rokabio.com
authors: Kristin
authors: K
authors: Livezey
authors: Shannon
authors: S
authors: Kaplan
authors: Michele
authors: M
authors: Wisniewski
authors: Michael M
authors: MM
authors: Becker
mesh_terms: D005516:Food Microbiology / Q000379:methods*; D005517:Foodborne Diseases / Q000382:microbiology*; D008087:Listeria / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis; D012335:RNA, Ribosomal; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal