title: Nucleotide and phylogenetic analysis of human papillomavirus types 6 and 11 isolated from recurrent respiratory papillomatosis in Brazil.
abstract: There are few studies about the distribution of natural molecular variants of low-risk HPVs. Our aim was to evaluate the E6 early gene variability among HPV-6 and HPV-11 isolates detected in recurrent respiratory papillomatosis (RRP) samples obtained in a cohort of Brazilian patients. We also performed a phylogenetic analysis in order to compare nucleotide sequences identified in our study with previously reported isolates from different anatomic sites (laryngeal papillomas, genital warts, cervical cancer and anal swabs) obtained from other parts of the world to determine the phylogenetic relationships of variants detected in Brazil. The complete coding region of the E6 gene of 25 samples was cloned and sequenced: 18 isolates of HPV-6 (72%) and 7 isolates of HPV-11 (28%). A total of four different HPV-6 genomic variants and two HPV-11 genomic variants was identified. It was not possible to correlate specific variants with disease severity. Phylogenetic trees for both HPV types were constructed enclosing both E6 sequences detected in our study and formerly published sequences. In both phylogenetic trees, the sequences from Brazil did not group together. We could not establish a geographical association between HPV-6 or HPV-11 variants, unlike HPV-16 and HPV-18.
authors: UNESP - São Paulo State University, IBILCE, Institute of Bioscience, Language & Literature and Exact Science, Department of Biology, Rua Cristóvão Colombo 2265, Bairro Jardim Nazareth, CEP 15054-010, São José do Rio Preto, São Paulo, Brazil. renatapram@hotmail.com
authors: Renata Prandini Adum
authors: RP
authors: de Matos
authors: Laura
authors: L
authors: Sichero
authors: Isabela Mazuco
authors: IM
authors: Mansur
authors: Caroline Measso
authors: CM
authors: do Bonfim
authors: Cíntia
authors: C
authors: Bittar
authors: Rodrigo Lacerda
authors: RL
authors: Nogueira
authors: Daniel Salgado
authors: DS
authors: Küpper
authors: Fabiana Cardoso Pereira
authors: FC
authors: Valera
authors: Maurício Lacerda
authors: ML
authors: Nogueira
authors: Luisa Lina
authors: LL
authors: Villa
authors: Marilia Freitas
authors: MF
authors: Calmon
authors: Paula
authors: P
authors: Rahal
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D005260:Female; D052140:Human papillomavirus 11 / Q000145:classification / Q000235:genetics*; D052163:Human papillomavirus 6 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins