title: The low dose gamma ionising radiation impact upon cooperativity of androgen-specific proteins.
abstract: The paper deals with effects of the ionising radiation (γ-IR, 0.5 Gy) upon serum testosterone (T), characteristics of testosterone-binding globulin (TeBG) and androgen receptor (AR) in parallel with observation of androgen (A) responsive enzyme activity - hexokinase (HK). The interdependence or relationships of T-levels with parameters of the proteins that provide androgenic regulation are consequently analyzed in post-IR dynamics. The IR-stress adjustment data reveal expediency of TeBG- and AR-cooperativity measurements for more precise assessments of endocrine A-control at appropriate emergencies.
authors: Lab. of Biochemistry, Institute of Radiobiology, National Academy of Sciences of Belarus, Feduninskogo 4, Gomel 246007, Belarus.
authors: Gennady N
authors: GN
authors: Filchenkov
authors: Eugene H
authors: EH
authors: Popoff
authors: Alexander D
authors: AD
authors: Naumov
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006593:Hexokinase / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000097:blood*; D012738:Sex Hormone-Binding Globulin / Q000032:analysis; D013739:Testosterone / Q000097:blood
chemical_list: D011944:Receptors, Androgen; D012738:Sex Hormone-Binding Globulin; D013739:Testosterone; D006593:Hexokinase
keywords: (h)TeBG; 5α-dihydrotestosterone; A; ABP; AC; AR; ARC; Androgenic receptor; Androgens; B; B(max); B(nsp); B(t); ChNPP; Chernobyl nuclear power plant; DCC; DF; DHT; Hb; Hexokinase; IR; ISS; International Space Station; Ionising radiation; K(a); K(d); MF; MT; OF; PM; SIS; T; T(add); Testosterone-binding globulin; U; aa; adenylate cyclase; amino acids; androgen receptor; androgen receptor complex; androgen(s); androgen-binding protein of rat i.e. (r)TeBG; dextran-coated charcoal (1:9); dimerization factor; equilibrium association constant; equilibrium dissociation constant; external plasma(tic) membrane of cell; final concentration of [(3)H]-DHT added in incubation system; hemoglobin; human testosterone-binding globulin; ionising radiation; maximal B′ value calculated by approximation in Scatchard plot; methyltrienolone; monomerization factor; nonspecific binding of A; oligomerization factor; quantity of specifically bound A ([(3)H]-DHT by TeBG and [(3)H]-MT by RA); standard incubation system; testosterone; the Hill coefficient indicating a degree of cooperativity for A-binding by TeBG or AR (calculated in Hill plot, see Figs. 2 and 3); total binding of [(3)H]-A; unbound [(3)H]-A that removed by DCC after incubation; η(Hill)