title: Altered pregnancy outcomes in mice following treatment with the hyperglycaemia mimetic, glucosamine, during the periconception period.
abstract: Exposure of cumulus-oocyte complexes to the hyperglycaemia mimetic, glucosamine, during in vitro maturation impairs embryo development, potentially through upregulation of the hexosamine biosynthesis pathway. This study examined the effects of in vivo periconception glucosamine exposure on reproductive outcomes in young healthy mice, and further assessed the effects in overweight mice fed a high-fat diet. Eight-week-old mice received daily glucosamine injections (20 or 400mgkg(-1)) for 3-6 days before and 1 day after mating (periconception). Outcomes were assessed at Day 18 of gestation. Glucosamine treatment reduced litter size independent of dose. A high-fat diet (21% fat) for 11 weeks before and during pregnancy reduced fetal size. No additional effects of periconception glucosamine (20mgkg(-1)) on pregnancy outcomes were observed in fat-fed mice. In 16-week-old mice fed the control diet, glucosamine treatment reduced fetal weight and increased congenital abnormalities, but did not alter litter size. As differing effects of glucosamine were observed in 8-week-old and 16-week-old mice, maternal age effects were assessed. Periconception glucosamine at 8 weeks reduced litter size, whereas glucosamine at 16 weeks reduced fetal size. Thus, in vivo periconception glucosamine exposure perturbs reproductive outcomes in mice, with the nature of the outcomes dependent upon maternal age.
authors: The Robinson Institute, The Research Centre for Reproductive Health, School of Paediatrics and Reproductive Health, The University of Adelaide, Adelaide, SA 5005, Australia.
authors: Cheryl J
authors: CJ
authors: Schelbach
authors: Rebecca L
authors: RL
authors: Robker
authors: Brenton D
authors: BD
authors: Bennett
authors: Ashley D
authors: AD
authors: Gauld
authors: Jeremy G
authors: JG
authors: Thompson
authors: Karen L
authors: KL
authors: Kind
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D049628:Body Size / Q000187:drug effects*; D059305:Diet, High-Fat / Q000009:adverse effects; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005865:Gestational Age; D005944:Glucosamine / Q000494:pharmacology*; D005951:Glucose Tolerance Test; D059471:In Vitro Oocyte Maturation Techniques; D007328:Insulin / Q000097:blood; D008098:Litter Size / Q000187:drug effects*; D008423:Maternal Age; D051379:Mice; D008810:Mice, Inbred C57BL; D050177:Overweight / Q000503:physiopathology*; D011247:Pregnancy; D011256:Pregnancy Outcome
chemical_list: D007328:Insulin; D005944:Glucosamine