title: Bridging HIV-1 cellular latency and clinical long-term non-progressor: an interactomic view.
abstract: Development of an effective HIV management is enticed by the fact that long-term non-progressors (LTNP) restrict viral replication spontaneously, but is hindered by HIV-1 latency. Given that the most overlapping characteristics found between HIV-1 LTNP and latency, detailed analysis of the difference would disclose the essentials of latency. In this study, microarray data from our previous study was combined with HIV-1 latency and LTNP data obtained from NCBI GEO database. Principal variance component analysis and hierarchical clustering verified the removal of batch effect across platform. The analysis revealed a total of 456 differential expressed genes with >2-fold change and B-statistic >0. Bayesian inference was used to reconstitute the transcriptional network of HIV-1 latency or LTNP, respectively. Gene regulation was reprogrammed under different disease condition. By network interference, KPNA2 and ATP5G3 were identified as the hubs in latency network which mediate nuclear export and RNA processing. These data offer comparative insights into HIV-1 latency, which will facilitate the understanding of the genetic basis of HIV-1 latency in vivo and serve as a clue for future treatment dealing with key targets in HIV-1 latency.
authors: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases, The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University, Hangzhou, China.
authors: Jin
authors: J
authors: Yang
authors: Zongxing
authors: Z
authors: Yang
authors: Hangjun
authors: H
authors: Lv
authors: Yi
authors: Y
authors: Lou
authors: Juan
authors: J
authors: Wang
authors: Nanping
authors: N
authors: Wu
mesh_terms: D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000502:physiology; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D006801:Humans; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D017735:Virus Latency / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
chemical_list: D014764:Viral Proteins