title: Characterization of the complete mitochondrial genome of Bombyx mori strain H9 (Lepidoptera: Bombycidae).
abstract: The complete mitochondrial genome (mitogenome) of Bombyx mori strain H9 (Lepidoptera: Bombycidae) is 15,670base pairs (bp) in length, encoding 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and a control region. The nucleotide composition of the genome is highly A+T biased, accounting for 81.31%, with a slightly positive AT skewness (0.059). The arrangement of 13 PCGs is similar to that of other sequenced lepidopterans. All the PCGs are initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which is proposed by the TTAG sequence as observed in other lepidopterans. Unlike the other PCGs, the cox1 and cytochrome c oxidase subunit 2 (cox2) genes have incomplete stop codons consisting of just a T. All tRNAs have typical structures of insect mitochondrial tRNAs, which is different from other sequenced lepidopterans. The structure of A+T-rich region is similar to that of other sequenced lepidopterans, including non-repetitive sequences, the ATAGA binding domain, a 18bp poly-T stretch and a poly-A element upstream of transfer RNA M (trnM) gene. Phylogenetic analysis shows that the domesticated silkmoth B. mori originated from the Chinese Bombyx mandarina.
authors: College of Life Science, Anhui Agricultural University, 130 Changjiang West Road 230036, PR China.
authors: Li-Shang
authors: LS
authors: Dai
authors: Bao-Jian
authors: BJ
authors: Zhu
authors: Qiu-Ning
authors: QN
authors: Liu
authors: Guo-Qing
authors: GQ
authors: Wei
authors: Chao-Liang
authors: CL
authors: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D018388:Codon, Terminator; D004272:DNA, Mitochondrial / Q000235:genetics; D019143:Evolution, Molecular; D049750:Genome, Insect; D054629:Genome, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018388:Codon, Terminator; D004272:DNA, Mitochondrial; D012343:RNA, Transfer