title: HIV-1 envelope trimer has similar binding characteristics for carbohydrate-binding agents as monomeric gp120.
abstract: The native HIV-1 Env complex consists of a gp120/gp41 trimer, but surface plasmon resonance (SPR)-directed binding studies for gp120-binding agents were almost exclusively performed on monomeric gp120. SPR-directed binding kinetics of monomeric gp120 and trimeric gp140 were investigated for a broad variety of envelope (Env)-binding agents. Similar kinetics for carbohydrate-binding agents (CBAs), the antibody 2G12 and sCD4 were observed, irrespective of the oligomeric state of gp120 that either contain the native mixture of complex and high-mannose N-glycans or that contain exclusively oligomannose N-glycans. The generally comparable kinetic properties of CBA, 2G12 and sCD4 binding to monomeric gp120 and trimeric gp140 indicate that monomeric gp120 is a good surrogate molecule for native HIV-1 Env trimer to investigate the binding affinities of Env-binding compounds.
authors: Rega Institute for Medical Research, KU Leuven, Leuven, Belgium. bart.hoorelbeke@rega.kuleuven.be
authors: Bart
authors: B
authors: Hoorelbeke
authors: Thijs
authors: T
authors: van Montfort
authors: Jie
authors: J
authors: Xue
authors: Patricia J
authors: PJ
authors: LiWang
authors: Haruo
authors: H
authors: Tanaka
authors: Yasuhiro
authors: Y
authors: Igarashi
authors: Els J M
authors: EJ
authors: Van Damme
authors: Rogier W
authors: RW
authors: Sanders
authors: Jan
authors: J
authors: Balzarini
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001667:Binding, Competitive; D015153:Blotting, Western; D000080908:Broadly Neutralizing Antibodies; D057809:HEK293 Cells; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008358:Mannose / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D020349:Surface Plasmon Resonance; D054299:env Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C529157:2G12 monoclonal antibody; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000080908:Broadly Neutralizing Antibodies; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D037121:Plant Lectins; D011134:Polysaccharides; C424136:actinohivin protein, actinomycete; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1; D008358:Mannose