title: Chromatin immunoprecipitation of adult murine cardiomyocytes.
abstract: This unit describes a streamlined two-step protocol for the isolation of adult murine cardiomyocytes with subsequent Chromatin ImmunoPrecipitation (ChIP). Isolation and culturing of cardiomyocytes is a delicate process and the protocol presented here optimizes the combination of cardiomyocyte isolation with ChIP. ChIP is an invaluable method for analyzing molecular interactions occurring between a specific protein (or its post-translationally modified form) and a region of genomic DNA. ChIP has become a widely used technique in the last decade since several groundbreaking studies have focused attention on epigenetics and have identified many epigenetic regulatory mechanisms. However, epigenetics within cardiovascular biology is a new area of focus for many investigators, and we have optimized a method for performing ChIP in adult murine cardiomyocytes, as we feel this will be an important aid to both the cardiovascular field and for the development of cell- and tissue-specific ChIP.
authors: Department of Cardiology, Mount Sinai School of Medicine, New York, New York.
authors: P
authors: P
authors: Bolli
authors: Departments of Oncological Sciences and Dermatology, Mount Sinai School of Medicine, New York, New York.
authors: C
authors: C
authors: Vardabasso
authors: Departments of Oncological Sciences and Dermatology, Mount Sinai School of Medicine, New York, New York.
authors: E
authors: E
authors: Bernstein
authors: Department of Cardiology, Mount Sinai School of Medicine, New York, New York.
authors: H W
authors: HW
authors: Chaudhry
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D051379:Mice; D032383:Myocytes, Cardiac / Q000378:metabolism*