title: Comparison of the genome-wide DNA methylation profiles between fast-growing and slow-growing broilers.
abstract: BACKGROUND
  Growth traits are important in poultry production, however, little is known for its regulatory mechanism at epigenetic level. Therefore, in this study, we aim to compare DNA methylation profiles between fast- and slow-growing broilers in order to identify candidate genes for chicken growth. Methylated DNA immunoprecipitation-sequencing (MeDIP-seq) was used to investigate the genome-wide DNA methylation pattern in high and low tails of Recessive White Rock (WRR(h); WRR(l)) and that of Xinhua Chickens (XH(h); XH(l)) at 7 weeks of age. The results showed that the average methylation density was the lowest in CGIs followed by promoters. Within the gene body, the methylation density of introns was higher than that of UTRs and exons. Moreover, different methylation levels were observed in different repeat types with the highest in LINE/CR1. Methylated CGIs were prominently distributed in the intergenic regions and were enriched in the size ranging 200-300 bp. In total 13,294 methylated genes were found in four samples, including 4,085 differentially methylated genes of WRR(h) Vs. WRR(l), 5,599 of XH(h) Vs. XH(l), 4,204 of WRR(h) Vs. XH(h), as well as 7,301 of WRR(l) Vs. XH(l). Moreover, 132 differentially methylated genes related to growth and metabolism were observed in both inner contrasts (WRR(h) Vs. WRR(l) and XH(h) Vs. XH(l)), whereas 129 differentially methylated genes related to growth and metabolism were found in both across-breed contrasts (WRR(h) Vs. XH(h) and WRR(l) Vs. XH(l)). Further analysis showed that overall 75 genes exhibited altered DNA methylation in all four contrasts, which included some well-known growth factors of IGF1R, FGF12, FGF14, FGF18, FGFR2, and FGFR3. In addition, we validate the MeDIP-seq results by bisulfite sequencing in some regions.
  
  
  CONCLUSIONS
  This study revealed the global DNA methylation pattern of chicken muscle, and identified candidate genes that potentially regulate muscle development at 7 weeks of age at methylation level.
authors: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors: Yongsheng
authors: Y
authors: Hu
authors: Haiping
authors: H
authors: Xu
authors: Zhenhui
authors: Z
authors: Li
authors: Xuejuan
authors: X
authors: Zheng
authors: Xinzheng
authors: X
authors: Jia
authors: Qinghua
authors: Q
authors: Nie
authors: Xiquan
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D019295:Computational Biology; D018899:CpG Islands; D019175:DNA Methylation; D057890:Epigenomics; D005260:Female; D005786:Gene Expression Regulation; D016678:Genome; D055106:Genome-Wide Association Study; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D019655:Quantitative Trait, Heritable; D015203:Reproducibility of Results