title: Bead-based padlock rolling circle amplification for single DNA molecule counting.
abstract: Padlock rolling circle amplification (RCA) is a powerful analytical method for ultrasensitive DNA detection. Although there are some advantages to bead-based RCA, a detailed study of the relationship between the bead material and the efficiency of bead-based RCA has not been reported. Here, we compared the reaction efficiencies of bead-based RCA performed on two types of bead material: agarose and polystyrene. Agarose was a more suitable material for on-bead RCA. The calibration curve showed linearity between 0.05 and 1 nM, and the limit of detection was 9 pM (9 amol) for Salmonella DNA determination.
authors: Department of Chemical and Biological Sciences, Japan Women's University, Bunkyou-ku, Tokyo, Japan. satouk@fc.jwu.ac.jp
authors: Kae
authors: K
authors: Sato
authors: Reina
authors: R
authors: Ishii
authors: Naoki
authors: N
authors: Sasaki
authors: Kiichi
authors: K
authors: Sato
authors: Mats
authors: M
authors: Nilsson
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D008863:Microspheres; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012475:Salmonella / Q000235:genetics
chemical_list: D004269:DNA, Bacterial