title: Raf kinase inhibitor protein expression combined with peritoneal involvement and lymphovascular invasion predicts prognosis in Dukes' B colorectal cancer patients.
abstract: OBJECTIVE
  There is controversy regarding the use of adjuvant therapy in patients with Dukes' B colorectal cancer (CRC). New markers, identifying high-risk Dukes' B patients, are needed. Here, we examine the utility of Raf kinase inhibitor protein (RKIP) as such a marker and promoter methylation as a mechanism of RKIP down-regulation.
  
  
  RESULTS
  We used a tissue microarray of 220 patients with Dukes' B CRC to examine the effect of RKIP expression on survival. Pyrosequencing was used to assess RKIP promoter methylation status.RKIP expression correlated inversely with disease-specific survival in this cohort. In multivariate analysis, RKIP was found to be an independent prognostic indicator, along with peritoneal invasion and lymphovascular invasion (LVI). RKIP promoter hypermethylation was seen in only one of 29 tumours analysed by pyrosequencing.
  
  
  CONCLUSIONS
  Raf kinase inhibitor protein, peritoneal invasion and LVI provide independent prognostic information in this cohort of Dukes' B CRC patients.This demonstrates the potential utility of RKIP in identifying 'high-risk' Dukes' B patients. It is this high-risk group which is most likely to benefit from close postoperative monitoring and may derive the most benefit from adjuvant therapy.
authors: Beatson Institute for Cancer Research, Glasgow, UK.
authors: Brendan
authors: B
authors: Doyle
authors: Suzanne
authors: S
authors: Hagan
authors: Fahd
authors: F
authors: Al-Mulla
authors: Lucy
authors: L
authors: Scott
authors: Sharon
authors: S
authors: Harden
authors: Jim
authors: J
authors: Paul
authors: Hugh
authors: H
authors: Mulcahy
authors: Graeme I
authors: GI
authors: Murray
authors: Kieran
authors: K
authors: Sheahan
authors: Jacintha
authors: J
authors: O'Sullivan
authors: Walter
authors: W
authors: Kolch
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D019175:DNA Methylation / Q000235:genetics; D015536:Down-Regulation; D006801:Humans; D053208:Kaplan-Meier Estimate; D009367:Neoplasm Staging; D049488:Phosphatidylethanolamine Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; C511482:PEBP1 protein, human; D049488:Phosphatidylethanolamine Binding Protein