title: Protective effect of paraoxonase 1 gene variant L55M in retinal vein occlusion.
abstract: OBJECTIVE
  To determine if the paraoxonase 1 L55M and paraoxonase 1 Q192R gene polymorphisms have an effect on the risk of having a retinal vein occlusion (RVO).
  
  
  METHODS
  This case-control prospective study included 120 patients with RVO and 84 control subjects. All subjects were screened for age, gender, hypertension, diabetes, body mass index, fibrinogen, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, total cholesterol, and very low-density lipoprotein. Subjects were also questioned about their smoking habits. Genomic DNA was extracted from peripheral leukocytes from EDTA anticoagulated blood. Genotyping of the paraoxonase 1 L55M and paraoxonase 1 Q192R polymorphisms was performed using real-time PCR.
  
  
  RESULTS
  The frequency of the paraoxonase 1 (PON1) 55 LL genotype was significantly lower in patients with RVO than in the control subjects (28% versus 55%; p = 0.005). Logistic regression analyses were also conducted. After adjusting for gender, diabetes, hypertension, plasma fibrinogen levels, and high-density lipoprotein cholesterol, the lower LL genotype was found to be an independent predictor of RVO (β = 1.755; odds ratio = 5.783; p < 0.001; 95% confidence interval = 2.579-12.967).
  
  
  CONCLUSIONS
  Subjects with a lower frequency PON1 55 LL genotype had a higher risk of RVO. These results indicate that paraoxonase gene polymorphisms may be a possible risk factor for RVO. We suggest that the LL genotype may have a protective role in the pathogenesis of RVO in the Turkish population.
authors: Gaziosmanpasa University Faculty of Medicine, Department of Ophthalmology, Tokat, Turkey. huseyin.ortak@hotmail.com
authors: Huseyin
authors: H
authors: Ortak
authors: Erkan
authors: E
authors: Söğüt
authors: Omer
authors: O
authors: Ateş
authors: Unal
authors: U
authors: Erkorkmaz
authors: Ismail
authors: I
authors: Benli
authors: Ali
authors: A
authors: Akbas
authors: Selim
authors: S
authors: Demir
authors: Hüseyin
authors: H
authors: Ozyurt
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution / Q000235:genetics; D043303:Aryldialkylphosphatase / Q000235:genetics*; D016022:Case-Control Studies; D005006:Ethnicity / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012170:Retinal Vein Occlusion / Q000201:enzymology* / Q000209:etiology / Q000235:genetics*; D012307:Risk Factors; D014421:Turkey / Q000208:ethnology
chemical_list: D043303:Aryldialkylphosphatase; C468406:PON1 protein, human