title: Preparation and characterization of chitosan/β-cyclodextrin nanoparticles containing plasmid DNA encoding interleukin-12.
abstract: Interleukin-12 (IL-12) as a cytokine has been proved to possess antitumor effects via stimulating the immune system. Non-viral gene delivery systems offer several advantages, including easiness in production, low cost, safety; low immunogenicity and can carry higher amounts of genetic material without limitation on their sizes.pUMVC3-hIL12 loaded Low Molecular Weight chitosan/β-cyclodextrin (LMW CS/CD) nanoparticles were prepared using ionotropic gelation method and characterized in terms of size, zeta potential, polydispersity index, morphology, loading efficiency and cytotoxicity against the CT-26 colon carcinoma cell line.All prepared particles were spherical in shape and nano-sized (171.3±2.165 nm, PdI: 0.231±0.014) and exhibited a positive zeta potential (34.3±1.55). The nanoparticles demonstrated good DNA encapsulation efficiencies (83.315%±2.067). Prepared pUMVC3-hIL12 loaded LMW CS/CD nanoparticles showed no cell toxicity in murine CT-26 colon carcinoma cells. At the concentration of 0.1 µg/ml of nanoparticles, the transfection ability was obviously higher than that of the naked DNA.LMW CS/CD-plasmid DNA nanoparticles encoding IL-12 prepared using ionotropic gelation method with no toxic effect on the tested cells can be considered as a basis for further gene delivery studies both in vitro and in vivo to enhance the expression of IL-12.
authors: Hematology and Oncology Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: M
authors: M
authors: Nahaei
authors: H
authors: H
authors: Valizadeh
authors: B
authors: B
authors: Baradaran
authors: M R
authors: MR
authors: Nahaei
authors: D
authors: D
authors: Asgari
authors: S
authors: S
authors: Hallaj-Nezhadi
authors: S
authors: S
authors: Dastmalchi
authors: F
authors: F
authors: Lotfipour
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000008:administration & dosage*; D004247:DNA / Q000008:administration & dosage*; D018664:Interleukin-12 / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight; D053758:Nanoparticles / Q000008:administration & dosage*; D010957:Plasmids; D014162:Transfection; D047392:beta-Cyclodextrins / Q000008:administration & dosage*
chemical_list: D047392:beta-Cyclodextrins; C571277:chitosan-beta-cyclodextrin; D018664:Interleukin-12; D004247:DNA; D048271:Chitosan