title: An old method facing a new challenge: re-visiting housekeeping proteins as internal reference control for neuroscience research.
abstract: The study of specific target protein expression is often performed by western blotting, a commonly used method to measure the protein expression in neuroscience research by specific antibodies. Housekeeping proteins are used as an internal control for protein loading as well as reference in the western blotting analysis. This practice is based on the belief that such housekeeping genes are considered to be ubiquitously and constitutively expressed in every tissue and produce the minimal essential transcripts necessary for normal cellular function. The most commonly used housekeeping proteins are β-actin, β-tubulin, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). However, recent studies have shown significant variation in some housekeeping genes both at the mRNA and protein levels in various neuropathological events, such as spinal cord injury and Alzheimer's diseases. Changes of housekeeping genes are also induced by non-neuronal diseases in various tissues. Therefore, these discoveries raise a potential concern regarding whether using a housekeeping protein as an internal standard for target protein analysis is an appropriate practice. This minireview will focus on (I) the effects of neuronal and non-neuronal diseases, experimental condition, and tissue-specific roles on alteration of housekeeping genes, and (II) alternative internal standards for gene and protein expression analysis.
authors: Center for Hormone Advanced Science and Education, Roskamp Institute, Sarasota, FL 34243, USA. rli@rfdn.org
authors: Rena
authors: R
authors: Li
authors: Yong
authors: Y
authors: Shen
mesh_terms: D000199:Actins / Q000032:analysis / Q000235:genetics; D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D020043:Genes, Essential; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D009488:Neurosciences / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000235:genetics*; D014404:Tubulin / Q000032:analysis / Q000235:genetics
chemical_list: D000199:Actins; D011506:Proteins; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases