title: Impacts of maternal selenium supply and nutritional plane on visceral tissues and intestinal biology in 180-day-old offspring in sheep.
abstract: Objectives were to investigate the effects of maternal Se supply and nutritional plane during gestation on offspring visceral tissues and indices of intestinal growth, vascularity, and function at 180 d of age. Rambouillet ewe lambs (n = 82, approximately 240 d of age; 52 ± 0.8 kg BW at breeding) were allocated to a 2 × 3 factorial arrangement of treatments. Treatments included dietary Se [adequate Se (ASe, 9.5 µg/kg BW) or high Se (HSe, 81.8 µg/kg BW)] initiated at breeding and nutritional plane [60% (restricted, RES), 100% (control, CON), and 140% (high, HI) of requirements] initiated at d 50 of gestation. Ewes were fed pelleted diets and housed individually indoors. At parturition, lambs were immediately removed and fed artificial colostrum for the first 20 h followed by ad libitum access to milk replacer. At 180 ± 2 d of age, lambs were euthanized and tissues were harvested. Birth weight was affected by nutritional treatments (P < 0.001), with decreased birth weight in RES and HI compared with CON. Offspring from RES and HI ewes had decreased (P = 0.07) blood volume compared with CON, and those born to HSe ewes had increased (P < 0.04) total visceral adiposity. Within offspring from CON ewes, those from HSe ewes had greater (P < 0.02) intestinal mass compared with ASe ewes. Within offspring from HSe ewes, both RES and HI had reduced (P ≤ 0.05) intestinal mass compared with CON. Jejunal capillary area density was greater (P = 0.08) in offspring from ewes fed HSe compared with ASe. In addition, area per capillary was greater (P ≤ 0.09) in CON compared with RES. Maternal nutritional plane tended (P ≤ 0.11) to alter total small intestinal vascularity, with lambs from CON being greater than RES. Expression of most mRNA for measured angiogenic factors and receptors was not altered (P ≤ 0.13) by maternal treatments; however, expression of glucagon-like peptide-2 (GLP-2) was decreased (P = 0.07) in offspring from RES compared with CON ewes. Offspring from ewes fed HI diets had increased (P = 0.08) jejunal mucosal maltase activity. In conclusion, maternal Se supply and nutritional plane during gestation resulted in measurable changes in offspring visceral tissues and intestinal biology, including perirenal fat, blood volume, intestinal mass, total jejunal crypt cell proliferation, area per capillary in jejunal villi, GLP-2 mRNA expression, and maltase activity at 180 d. Additional work is needed to determine impacts on intestinal function and nutrient uptake.
authors: Department of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108, USA.
authors: R D
authors: RD
authors: Yunusova
authors: T L
authors: TL
authors: Neville
authors: K A
authors: KA
authors: Vonnahme
authors: C J
authors: CJ
authors: Hammer
authors: J J
authors: JJ
authors: Reed
authors: J B
authors: JB
authors: Taylor
authors: D A
authors: DA
authors: Redmer
authors: L P
authors: LP
authors: Reynolds
authors: J S
authors: JS
authors: Caton
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D053767:Glucagon-Like Peptide 2 / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007422:Intestines / Q000033:anatomy & histology* / Q000187:drug effects / Q000201:enzymology; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D039382:Maternal Nutritional Physiological Phenomena; D009752:Nutritional Status; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D018027:Receptors, Glucagon / Q000235:genetics / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage / Q000378:metabolism*; D034561:Sheep, Domestic / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology*
chemical_list: D053767:Glucagon-Like Peptide 2; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D018027:Receptors, Glucagon; D017978:Receptors, Growth Factor; D012643:Selenium
keywords: developmental programming; glucagon-like peptide-2; intestinal growth; lambs; vascularization