title: The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile.
abstract: We have examined the polar lipids of Clostridium psychrophilum, a recently characterized psychrophilic Clostridium isolated from an Antarctic microbial mat. Lipids were extracted from cells grown near the optimal growth temperature (+5°C) and at -5°C, and analyzed by two-dimensional thin layer chromatography and liquid chromatography coupled with mass spectrometry. The major phospholipids of this species are: cardiolipin, phosphatidylethanolamine, and phosphatidylglycerol. Phosphatidylserine and lyso-phosphatidylethanolamine were found as minor components. The most abundant glycolipids are a monoglycosyldiradylglycerol (MGDRG) and a diglycosyldiradylglycerol (DGDRG). The latter was only seen in cells grown at -5°C. An ethanolamine-phosphate derivative of N-acetylglucosaminyldiradylglycerol was seen in cells grown at -5°C and an ethanolamine-phosphate derivative of MGDRG was found in cells grown at +5°C. All lipids were present in both the all acyl and plasmalogen (alk-1'-enyl acyl) forms with the exception of PS and MGDRG, which were predominantly in the diacyl form. The significance of lipid changes at the two growth temperatures is discussed.
authors: Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
authors: Ziqiang
authors: Z
authors: Guan
authors: Bing
authors: B
authors: Tian
authors: Amedea
authors: A
authors: Perfumo
authors: Howard
authors: H
authors: Goldfine
mesh_terms: D002308:Cardiolipins / Q000032:analysis; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000032:analysis; D010718:Phosphatidylserines / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
chemical_list: D002308:Cardiolipins; D008055:Lipids; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; C483858:phosphatidylethanolamine