title: A translocation-defective telomerase with low levels of activity and processivity stabilizes short telomeres and confers immortalization.
abstract: Short, repetitive, G-rich telomeric sequences are synthesized by telomerase, a ribonucleoprotein consisting of telomerase reverse transcriptase (TERT) and an integrally associated RNA. Human TERT (hTERT) can repetitively reverse transcribe its RNA template, acting processively to add multiple telomeric repeats onto the same substrate. We investigated whether certain threshold levels of telomerase activity and processivity are required to maintain telomere function and immortalize human cells with limited lifespan. We assessed hTERT variants with mutations in motifs implicated in processivity and interaction with DNA, namely the insertion in fingers domain (V791Y), and the E primer grip motif (W930F). hTERT-W930F and hTERT-V791Y reconstitute reduced levels of DNA synthesis and processivity compared with wild-type telomerase. Of interest, hTERT-W930F is more defective in translocation than hTERT-V791Y. Nonetheless, hTERT-W930F, but not hTERT-V791Y, immortalizes limited-lifespan human cells. Both hTERT-W930F- and hTERT-V791Y-expressing cells harbor short telomeres, measured as signal free ends (SFEs), yet SFEs persist only in hTERT-V791Y cells, which undergo apoptosis, likely as a consequence of a defect in recruitment of hTERT-V791Y to telomeres. Our study is the first to demonstrate that low levels of DNA synthesis--on the order of 20% of wild-type telomerase levels--and extension of as few as three telomeric repeats are sufficient to maintain functional telomeres and immortalize limited-lifespan human cells.
authors: Department of Anatomy and Cell Biology, McGill University, Montréal, PQ H3A 2B2, Canada.
authors: Yasmin
authors: Y
authors: D'Souza
authors: Tsz Wai
authors: TW
authors: Chu
authors: Chantal
authors: C
authors: Autexier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D002474:Cell-Free System; D004247:DNA / Q000096:biosynthesis; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011817:Rabbits; D019098:Telomerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism; D059505:Telomere Homeostasis; D014178:Translocation, Genetic
chemical_list: D004247:DNA; C509186:TERT protein, human; D019098:Telomerase