title: [Effects of serum anti-sperm antibodies positive on the Fas/Fas-L apoptosis pathway in the testis tissue and testicular germ cells of pubertal male rats].
abstract: OBJECTIVE
  To establish a rat model of anti-sperm antibody (AsAb)-mediated immune infertility, and investigate the effects of serum AsAb positive on the Fas/Fas-L apoptosis pathway in testis tissue and testicular germ cells of pubertal male rats.
  
  
  METHODS
  Thirty 5-week-old Wistar male rats were included in this study, 10 killed for preparation of sperm suspension, 10 as normal controls, and the other 10 made models of AsAb-positive immune infertility (experimental group). Four weeks after modeling, the testes of the rats were harvested for observation of the changes in the testis tissue under the light microscope and detection of the expressions of Fas, Fas-L and Caspase-3 proteins by immunohistochemistry.
  
  
  RESULTS
  Compared with the control group, the experimental group showed obvious apoptotic changes in the testis tissue and remarkably increased expressions (OD value) of Fas (161.87 +/- 5.37 vs 176.97 +/- 4.58), Fas-L (150.27 +/- 8.65 vs 187.52 +/- 7.76) and Caspase-3 (120.37 +/- 6.76 vs 157.65 +/- 7.38) (P < 0.01).
  
  
  CONCLUSIONS
  Serum AsAb affected the infertility of pubertal male rats, and its mechanisms might be associated with up-regulated expression of Fas, Fas-L and Caspase-3 proteins in the Fas/Fas-L apoptotic pathway.
authors: Department of Urology, The First Affiliated Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China.
authors: Er-Lei
authors: EL
authors: Zhi
authors: Ji-Xiu
authors: JX
authors: Xu
authors: Jing-Qi
authors: JQ
authors: Wang
authors: Ning
authors: N
authors: Xu
authors: Peng
authors: P
authors: Ji
authors: Zhuo
authors: Z
authors: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D001323:Autoantibodies / Q000276:immunology*; D053148:Caspase 3 / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism*; D005854:Germ Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D007248:Infertility, Male; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism
chemical_list: D001323:Autoantibodies; D053222:Fas Ligand Protein; C491898:Fas protein, rat; C505662:Faslg protein, rat; D019014:fas Receptor; C505410:Casp3 protein, rat; D053148:Caspase 3