title: Recognition and capture of metastatic hepatocellular carcinoma cells using aptamer-conjugated quantum dots and magnetic particles.
abstract: Metastatic recurrence is the most important biological behavior of hepatocellular carcinoma (HCC) and the main cause of treatment failure. Early prediction of metastasis is currently impossible due to the lack of specific molecular probes to recognize metastatic HCC cells. Aptamers have recently emerged as promising potential molecular probes for biomedical applications. Two well-matched HCC cell lines including HCCLM9 with high metastatic potential and MHCC97-L with low metastatic potential, were used to select aptamers for HCC metastasis. With a whole-cell-SELEX strategy, in which HCCLM9 cells were used as target cells and MHCC97-L cells as subtractive cell, 6 potential aptamers had been generated. Detailed study on selected aptamer LY-1 revealed that it could bind metastatic HCC cells with high affinity and specificity, not only in cells culture and animal models of HCC metastasis, but also in clinical HCC specimens. Moreover, the aptamer LY-1 and magnetic particles conjugates could efficiently capture the HCC cells from complex mixture whole blood. These studies demonstrated that this HCC specific aptamer LY-1 could be a promising molecular probe to recognize metastatic HCC cells.
authors: Department of Oncology, Zhongnan Hospital of Wuhan University, No 169 Donghu Road, Wuchang District, Wuhan 430071, PR China.
authors: Fu-Bing
authors: FB
authors: Wang
authors: Yuan
authors: Y
authors: Rong
authors: Min
authors: M
authors: Fang
authors: Jing-Ping
authors: JP
authors: Yuan
authors: Chun-Wei
authors: CW
authors: Peng
authors: Shao-Ping
authors: SP
authors: Liu
authors: Yan
authors: Y
authors: Li
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D019921:Biotinylation; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D008280:Magnetics / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015335:Molecular Probes; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D045663:Quantum Dots; D052156:SELEX Aptamer Technique; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D023041:Xenograft Model Antitumor Assays
chemical_list: D052157:Aptamers, Nucleotide; D015335:Molecular Probes; D016650:Fluorescein-5-isothiocyanate