title: [Keratinocyte growth factor and its receptor in middle-ear cholesteatoma].
abstract: OBJECTIVE
  Etiology of middle ear cholesteatoma is far from beeing elicidated. Current concepts postulate that cholesteatoma is the result of disturbed wound healing process. Keratinocyte growth factor (KGF) plays important role in wound healing, so it seemed interesting to assess the expression of KGF and its receptor in the cholesteatoma.
  
  
  METHODS
  25 cholesteatoma and 7 retraction pockets specimens were obtained from the patients during middle ear surgery. The expression of KGF and FGFR2 was evaluated by immunohistochemical staining using polyclonal antibodies. The results were analyzed depending on clinical features such as: localization, number of anatomic sites involved and presence of purulent discharge.
  
  
  RESULTS
  KGF expression was detected in the cells of subepithelial tissue. It was observed in 4/7 (57%) of the specimens of retraction pockets and 11/25 (44%) of cholesteatoma specimens. FGFR2 expression was observed mainly in the cells of spinous layer of epithelium in 4/7 (57%) specimens of retraction pockets and 19/25 (76%) of specimens of cholesteatoma. Statistically significant (p=0.011) relationship between the presence of KGF expression in the subepithelial tissue and the presence of FGFR2 expression in the epithelium was found. There were no differences in the expression of KGF and FGFR2 between retraction pocket and cholesteatoma specimens. Also, no differences in the expression, depending on localization, extend of the cholesteatoma and presence of purulent discharge were observed.
  
  
  CONCLUSIONS
  KGF and its receptor seem to play an important role in paracrine regulation of cholesteatoma growth.
authors: Zakład Patomorfologii Ogólnej Uniwersytetu Medycznego w Białymstoku, Poland.
authors: Katarzyna
authors: K
authors: Guzińska-Ustymowicz
authors: Agnieszka
authors: A
authors: Kurzyna
authors: Krzysztof
authors: K
authors: Trzpis
authors: Andrzej
authors: A
authors: Kemona
authors: Elżbieta
authors: E
authors: Hassmann-Poznańska
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism*; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000378:metabolism*; D006801:Humans; D008297:Male; D019899:Paracrine Communication; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D051523:Fibroblast Growth Factor 7; D051497:Receptor, Fibroblast Growth Factor, Type 2