title: Transcriptome responses to combinations of stresses in Arabidopsis.
abstract: Biotic and abiotic stresses limit agricultural yields, and plants are often simultaneously exposed to multiple stresses. Combinations of stresses such as heat and drought or cold and high light intensity have profound effects on crop performance and yields. Thus, delineation of the regulatory networks and metabolic pathways responding to single and multiple concurrent stresses is required for breeding and engineering crop stress tolerance. Many studies have described transcriptome changes in response to single stresses. However, exposure of plants to a combination of stress factors may require agonistic or antagonistic responses or responses potentially unrelated to responses to the corresponding single stresses. To analyze such responses, we initially compared transcriptome changes in 10 Arabidopsis (Arabidopsis thaliana) ecotypes using cold, heat, high-light, salt, and flagellin treatments as single stress factors as well as their double combinations. This revealed that some 61% of the transcriptome changes in response to double stresses were not predic from the responses to single stress treatments. It also showed that plants prioritized between potentially antagonistic responses for only 5% to 10% of the responding transcripts. This indicates that plants have evolved to cope with combinations of stresses and, therefore, may be bred to endure them. In addition, using a subset of this data from the Columbia and Landsberg erecta ecotypes, we have delineated coexpression network modules responding to single and combined stresses.
authors: Center for Biological Sequence Analysis , Technical University of Denmark, Lyngby, Denmark.
authors: Simon
authors: S
authors: Rasmussen
authors: Pankaj
authors: P
authors: Barah
authors: Maria Cristina
authors: MC
authors: Suarez-Rodriguez
authors: Simon
authors: S
authors: Bressendorff
authors: Pia
authors: P
authors: Friis
authors: Paolo
authors: P
authors: Costantino
authors: Atle M
authors: AM
authors: Bones
authors: Henrik Bjørn
authors: HB
authors: Nielsen
authors: John
authors: J
authors: Mundy
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects; D016000:Cluster Analysis; D003080:Cold Temperature; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D053263:Gene Regulatory Networks / Q000235:genetics; D008027:Light; D058977:Molecular Sequence Annotation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics* / Q000528:radiation effects; D059467:Transcriptome / Q000235:genetics* / Q000528:radiation effects
chemical_list: D012333:RNA, Messenger