title: Turn-on, fluorescent nuclear stains with live cell compatibility.
abstract: DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.
authors: Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33146, USA.
authors: Demar R G
authors: DR
authors: Pitter
authors: Jens
authors: J
authors: Wigenius
authors: Adrienne S
authors: AS
authors: Brown
authors: James D
authors: JD
authors: Baker
authors: Fredrik
authors: F
authors: Westerlund
authors: James N
authors: JN
authors: Wilson
mesh_terms: D001562:Benzimidazoles; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D007211:Indoles; D007834:Lasers; D008956:Models, Chemical; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D001562:Benzimidazoles; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA; C017807:bisbenzimide ethoxide trihydrochloride