title: Live-cell analysis of plant reproduction: live-cell imaging, optical manipulation, and advanced microscopy technologies.
abstract: Sexual reproduction ensures propagation of species and enhances genetic diversity within populations. In flowering plants, sexual reproduction requires complicated and multi-step cell-to-cell communications among male and female cells. However, the confined nature of plant reproduction processes, which occur in the female reproductive organs and several cell layers of the pistil, limits our ability to observe these events in vivo. In this review, we discuss recent live-cell imaging in in vitro systems and the optical manipulation techniques that are used to capture the dynamic mechanisms representing molecular and cellular communications in sexual plant reproduction.
authors: Division of Biological Science, Graduate School of Science, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan. kuri@bio.nagoya-u.ac.jp
authors: Daisuke
authors: D
authors: Kurihara
authors: Yuki
authors: Y
authors: Hamamura
authors: Tetsuya
authors: T
authors: Higashiyama
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D002450:Cell Communication; D005306:Fertilization; D035264:Flowers; D017343:Genes, Plant; D014644:Genetic Variation; D019684:Magnoliopsida / Q000235:genetics / Q000502:physiology; D008853:Microscopy / Q000379:methods*; D055095:Optics and Photonics; D010944:Plants; D053205:Pollen Tube / Q000378:metabolism*; D015398:Signal Transduction