title: Molecular-genetic variance of RH blood group system within human population of Bosnia and Herzegovina.
abstract: There are two major theories for inheritance of Rh blood group system: Fisher - Race theory and Wiener theory. Aim of this study was identifying frequency of RHDCE alleles in Bosnian - Herzegovinian population and introduction of this method in screening for Rh phenotype in B&H since this type of analysis was not used for blood typing in B&H before. Rh blood group was typed by Polymerase Chain Reaction, using the protocols and primers previously established by other authors, then carrying out electrophoresis in 2-3% agarose gel. Percentage of Rh positive individuals in our sample is 84.48%, while the percentage of Rh negative individuals is 15.52%. Inter-rater agreement statistic showed perfect agreement (K=1) between the results of Rh blood system detection based on serological and molecular-genetics methods. In conclusion, molecular - genetic methods are suitable for prenatal genotyping and specific cases while standard serological method is suitable for high-throughput of samples.
authors: Institute for Genetic Engineering and Biotechnology Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and Herzegovina. lejla.lasic@ingeb.ba
authors: Lejla
authors: L
authors: Lasić
authors: Naida
authors: N
authors: Lojo-Kadrić
authors: Elma
authors: E
authors: Silajdžić
authors: Lejla
authors: L
authors: Pojskić
authors: Rifat
authors: R
authors: Hadžiselimović
authors: Naris
authors: N
authors: Pojskić
mesh_terms: D017522:Bosnia and Herzegovina; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System