title: Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.
abstract: A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity to ferrozine and cobalt to obtain a subset of 34 oxygen-responsive candidate genes including the known hypoxic gene activator, MGA2. With each mutant in this subset a plasmid based β-galactosidase assay was performed using the anoxic-inducible promoter from OLE1 gene, and 17 gene deletions were identified that inhibit induction under anaerobic conditions. Genetic interaction analysis for one of these mutants, the RNase-encoding POP2 gene, revealed synthetic sick interactions with a number of genes involved in oxygen sensing and response. Knockdown experiments for CNOT8, human homolog of POP2, reduced cell survival under low oxygen condition suggesting a similar function in human cells.
authors: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6, Canada.
authors: Bahram
authors: B
authors: Samanfar
authors: Katayoun
authors: K
authors: Omidi
authors: Mohsen
authors: M
authors: Hooshyar
authors: Ben
authors: B
authors: Laliberte
authors: Md
authors: M
authors: Alamgir
authors: Andrew J
authors: AJ
authors: Seal
authors: Eman
authors: E
authors: Ahmed-Muhsin
authors: Duber Frey
authors: DF
authors: Viteri
authors: Kamaleldin
authors: K
authors: Said
authors: Firoozeh
authors: F
authors: Chalabian
authors: Ardeshir
authors: A
authors: Golshani
authors: Gabriel
authors: G
authors: Wainer
authors: Daniel
authors: D
authors: Burnside
authors: Kristina
authors: K
authors: Shostak
authors: Magdalena
authors: M
authors: Bugno
authors: William G
authors: WG
authors: Willmore
authors: Myron L
authors: ML
authors: Smith
authors: Ashkan
authors: A
authors: Golshani
mesh_terms: D015687:Cell Hypoxia; D002460:Cell Line; D002470:Cell Survival / Q000235:genetics; D003035:Cobalt / Q000494:pharmacology; D044943:Fatty Acid Desaturases / Q000235:genetics; D005297:Ferrozine / Q000494:pharmacology; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D007502:Iron Chelating Agents / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D010100:Oxygen / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012260:Ribonucleases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013230:Stearoyl-CoA Desaturase; D014131:Trace Elements / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: C489780:CNOT8 protein, human; D007502:Iron Chelating Agents; C542266:MGA2 protein, S cerevisiae; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D014131:Trace Elements; D014157:Transcription Factors; D005297:Ferrozine; D003035:Cobalt; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase; D012260:Ribonucleases; C079009:POP2 protein, S cerevisiae; D001616:beta-Galactosidase; D010100:Oxygen