title: Pharmacokinetics and residues of tetracycline in crucian carp muscle using capillary electrophoresis on-line coupled with electrochemiluminescence detection.
abstract: A novel and sensitive determination for tetracycline (TC) in crucian carp muscle was developed by using capillary electrophoresis coupled with electrochemiluminescence (ECL) detection. The conditions affected separation and detection were examined in detail. The linearity range of TC concentration was from 0.005 to 10 μg mL(-1) with a correlation coefficient of 0.9992. The detection limit of TC (S/N=3) was 1.8 ng mL(-1). The relative standard deviations of the ECL intensity and the migration time for eleven consecutive injections of 1.0 μg mL(-1) TC were 1.6% and 0.8%, respectively. The recovery of TC was 97.8% (n=5). After administration of 75 mg kg(-1) TC, the maximum concentration and peak time of TC in crucian carp muscle were 7.33 mg kg(-1) and 8h, respectively. After administration of TC, TC concentration demonstrated trivial variation in the period from 48 to 96 h.
authors: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. dengby16@163.com
authors: Biyang
authors: B
authors: Deng
authors: Quanxiu
authors: Q
authors: Xu
authors: Hua
authors: H
authors: Lu
authors: Li
authors: L
authors: Ye
authors: Yingzi
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000493:pharmacokinetics; D001331:Automation; D002347:Carps; D004350:Drug Residues / Q000032:analysis* / Q000493:pharmacokinetics; D019075:Electrophoresis, Capillary / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D008163:Luminescent Measurements / Q000379:methods*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D013752:Tetracycline / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline