title: Biosynthesis of silver nanoparticles by the endophytic fungus Epicoccum nigrum and their activity against pathogenic fungi.
abstract: There is an enormous interest in developing safe, cost-effective and environmentally friendly technologies for nano-materials synthesis. In the present study, extracellular biosynthesis of silver nanoparticles was achieved by Epicoccum nigrum, an endophytic fungus isolated from the cambium of Phellodendron amurense. The reduction of the silver ions was monitored by UV-visible spectrophotometry, and the characterization of the Ag NPs was carried out by X-ray diffraction and transmission electron microscopy. The synthesized Ag NPs were exceptionally stable. It was found that an alkaline pH favored the formation of Ag NPs and elevated temperature accelerated the reduction process. Furthermore, the antifungal activity of the Ag NPs was assessed using a microdilution method. The biosynthesized Ag NPs showed considerable activity against the pathogenic fungi. The current research opens a new path for the green synthesis of Ag NPs and the process is easy to scale up for biomedical applications.
authors: Department of Pathogenobiology, Norman Bethune College of Medicine, Jilin University Mycology Research Center, Jilin University, 126 Xinmin Street, Changchun, 130021, People's Republic of China.
authors: Yongqing
authors: Y
authors: Qian
authors: Huimei
authors: H
authors: Yu
authors: Dan
authors: D
authors: He
authors: Hui
authors: H
authors: Yang
authors: Wanting
authors: W
authors: Wang
authors: Xue
authors: X
authors: Wan
authors: Li
authors: L
authors: Wang
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000378:metabolism*; D001483:Base Sequence; D002176:Candida albicans / Q000187:drug effects*; D003455:Cryptococcus neoformans / Q000187:drug effects*; D017931:DNA Primers; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction
chemical_list: D000935:Antifungal Agents; D017931:DNA Primers; D012834:Silver