title: A practical and scalable manufacturing process for an anti-fungal agent, Nikkomycin Z.
abstract: A scalable and reliable manufacturing process for Nikkomycin Z HCl on a 170 g scale has been developed and optimized. The process is characterized by a 2.3 g/L fermentation yield, 79% purification yield, and >98% relative purity of the final product. This method is suitable for further scale up and cGMP production. The Streptomyces tendae ΔNikQ strain developed during the course of this study is superior to any previously reported strain in terms of higher yield and purity of Nikkomycin Z.
authors: Institute for Therapeutics Discovery and Development and Department of Medicinal Chemistry, University of Minnesota, Minneapolis, Minnesota 55414, United States ; BioTechnology Institute, University of Minnesota, Saint Paul, Minnesota 55108, United States.
authors: Christopher J
authors: CJ
authors: Stenland
authors: Lev G
authors: LG
authors: Lis
authors: Frederick J
authors: FJ
authors: Schendel
authors: Nicholas J
authors: NJ
authors: Hahn
authors: Mary A
authors: MA
authors: Smart
authors: Amy L
authors: AL
authors: Miller
authors: Marc G
authors: MG
authors: von Keitz
authors: Vadim J
authors: VJ
authors: Gurvich
keywords: Nikkomycin Z