title: Effect of asbestos exposure on differentiation of cytotoxic T lymphocytes in mixed lymphocyte reaction of human peripheral blood mononuclear cells.
abstract: Asbestos fibers are associated with tumorigenicity, and are thought to cause mesothelioma. However, their effect on immune response remains unclear. We examined the effect of asbestos exposure on differentiation of cytotoxic T lymphocytes (CTLs) in mixed lymphocyte reactions (MLR) of human peripheral blood mononuclear cells (PBMCs) upon exposure to chrysotile B (CB) or crocidolite (CR) asbestos at 5 μg/ml for 7 days. Exposure to CB during MLR suppressed increases in the percentage and number of CD8⁺ T cells in response to allogenic cells. The cytotoxicity for allogenic targets decreased in PBMCs exposed to CB, but not CR, when compared with PBMCs without any exposure during MLR. Exposure to CB during MLR resulted in suppression of increases in granzyme B⁺ cells and IFN-γ⁺ cells. CB exposure also resulted in suppression of increases in CD45RO⁺ effector/memory cells and CD25⁺-activated cells in CD8⁺ lymphocytes, and a decrease in CD45RA⁺ cells. CB exposure suppressed the proliferation of CD8⁺ lymphocytes without an increase in annexin V⁺ apoptotic cells in CD8⁺ lymphocytes. Moreover, the production of IL-10, IFN-γ, and TNF-α, but not IL-2, decreased in the presence of CB. These results suggest that exposure to asbestos potentially suppresses the differentiation of cytotoxic T lymphocyte, accompanied by decreases in IFN-γ and TNF-α.
authors: Department of Hygiene, Kawasaki Medical School, Kurashiki, Japan.
authors: Naoko
authors: N
authors: Kumagai-Takei
authors: Yasumitsu
authors: Y
authors: Nishimura
authors: Megumi
authors: M
authors: Maeda
authors: Hiroaki
authors: H
authors: Hayashi
authors: Hidenori
authors: H
authors: Matsuzaki
authors: Suni
authors: S
authors: Lee
authors: Junichi
authors: J
authors: Hiratsuka
authors: Takemi
authors: T
authors: Otsuki
mesh_terms: D017209:Apoptosis; D017638:Asbestos, Crocidolite / Q000009:adverse effects*; D017632:Asbestos, Serpentine / Q000009:adverse effects; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D018655:Lymphocyte Count; D007959:Lymphocyte Culture Test, Mixed; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017632:Asbestos, Serpentine; D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha; D017638:Asbestos, Crocidolite; D007371:Interferon-gamma; C509213:GZMB protein, human; D053804:Granzymes