title: Chondroitinase injection improves keloid pathology by reorganizing the extracellular matrix with regenerated elastic fibers.
abstract: Keloids are a proliferative fibrotic disease characterized by abnormal accumulation of extracellular matrix in the dermis. Keloid lesions lack skin plasticity due to deficiencies in elastic fiber formation in the extracellular matrix. The loss of elastic fiber is caused by excessive accumulation of chondroitin sulfate (CS), a sulfated glycosaminoglycan. However, there is no radical cure for keloids. Using a model system, we show herein that treatment of keloid tissues with chondroitinase ABC, an enzyme that specifically digests CS, improves clinical features of keloids. Keloid tissues obtained from patients were grafted on nude mice, and chondroitinase ABC was injected into the grafted keloid tissues. Chondroitinase ABC treatment significantly reduced the volume of keloid implants concomitant with recovery of elastic fiber formation. These results suggest that chondroitinase ABC injection is an effective therapy for keloid.
authors: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors: Toshihiro
authors: T
authors: Ishiko
authors: Motoko
authors: M
authors: Naitoh
authors: Hiroshi
authors: H
authors: Kubota
authors: Satoko
authors: S
authors: Yamawaki
authors: Mika
authors: M
authors: Ikeda
authors: Katsuhiro
authors: K
authors: Yoshikawa
authors: Hiroshi
authors: H
authors: Fujita
authors: Hiroaki
authors: H
authors: Yamaguchi
authors: Yasuhiro
authors: Y
authors: Kurahashi
authors: Shigehiko
authors: S
authors: Suzuki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D019765:Chondroitin ABC Lyase / Q000008:administration & dosage*; D004353:Drug Evaluation, Preclinical; D004547:Elastic Tissue / Q000473:pathology; D005109:Extracellular Matrix / Q000187:drug effects; D005260:Female; D006801:Humans; D015552:Injections, Intralesional; D007627:Keloid / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012038:Regeneration / Q000187:drug effects
chemical_list: D019765:Chondroitin ABC Lyase