title: Phenotypic analysis of ovine antigen presenting cells loaded with nanoparticles migrating from the site of vaccination.
abstract: Virus-sized particulate adjuvants such as ISCOMs, polystyrene nanoparticles and virus-like particles have been shown to target dendritic cells, resulting in the activation of T and B cells in vivo. Using an ovine pseudo-afferent lymph cannulation model to capture APC that traffic from the site of injection to the local lymph node, we show that 40-50 nm nanoparticles are taken up at the site of injection by dendritic cells (DCs) migrating to the draining lymph node. These DCs can express CD11c, CD1b, CD5, MHC class II and CD8. Nanoparticles transported by DCs migrating from the site of injection to the local lymph node therefore needs to be considered as a new mechanism underlying the immunogenicity of virus-sized vaccine delivery systems.
authors: Centre for Animal Biotechnology, Faculty of Veterinary Science, University of Melbourne, Victoria 3010, Australia.
authors: Anita
authors: A
authors: Gamvrellis
authors: Katrina
authors: K
authors: Walsh
authors: Liliana
authors: L
authors: Tatarczuch
authors: Peter
authors: P
authors: Smooker
authors: Magdalena
authors: M
authors: Plebanski
authors: Jean-Pierre Y
authors: JP
authors: Scheerlinck
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D015870:Gene Expression; D016882:ISCOMs / Q000008:administration & dosage / Q000276:immunology; D016130:Immunophenotyping; D008196:Lymph / Q000166:cytology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D053758:Nanoparticles / Q000737:chemistry*; D010047:Ovalbumin / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D034561:Sheep, Domestic; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014611:Vaccination
chemical_list: D015703:Antigens, CD; D004337:Drug Carriers; D016882:ISCOMs; D010047:Ovalbumin