title: Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization.
abstract: Members of the KLFs family of transcription factors play roles in maternal endometrium development during embryo implantation. However, the specific role of KLF12 in endometrium development has not yet been described. In this study, we showed that KLF12 expression in human endometrial stromal cells (HESCs) was significantly decreased after decidualization stimulated by 8-Br-cAMP and medroxyprogesterone acetate (MPA). The adenovirus-mediated overexpression of KLF12 in HESCs significantly repressed the expression and secretion of decidualization biomarker genes and their products decidual prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1) induced by 8-Br-cAMP and MPA. Moreover, CHIP and luciferase reporter assays demonstrated that KLF12 bound to a CAGTGGG element within the decidual prolactin promoter and decreased decidual PRL promoter (dPRL/-2000Luc) activation in a sequence-specific manner. Taken together, these findings suggest KLF12 is a negative regulator of human endometrial stromal cell decidualization.
authors: Reproductive Medicine Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, People's Republic of China.
authors: Xiaoyue
authors: X
authors: Shen
authors: Yali
authors: Y
authors: Hu
authors: Yue
authors: Y
authors: Jiang
authors: Hongyu
authors: H
authors: Liu
authors: Lihua
authors: L
authors: Zhu
authors: Xiaoyan
authors: X
authors: Jin
authors: Huizhi
authors: H
authors: Shan
authors: Xin
authors: X
authors: Zhen
authors: Lihua
authors: L
authors: Sun
authors: Guijun
authors: G
authors: Yan
authors: Haixiang
authors: H
authors: Sun
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites / Q000187:drug effects; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005819:Genetic Markers; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D011388:Prolactin / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D005819:Genetic Markers; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; C436492:KLF12 protein, human; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011388:Prolactin; D017258:Medroxyprogesterone Acetate