title: The antiviral effect of 7-hydroxyisoflavone against Enterovirus 71 in vitro.
abstract: Enterovirus 71 (EV71) is the major causative agent of hand foot and mouth disease. And EV71 causes epidemics worldwide, particularly in the Asia-Pacific region. Unfortunately, currently there is no approved vaccine or antiviral drug for EV71-induced disease prevention and therapy. In screening for anti-EV71 candidates, we found that 7-hydroxyisoflavone was active against EV71. 7-Hydroxyisoflavone exhibited strong antiviral activity against three different EV71 strains. The 50% inhibitory concentration range was between 3.25 and 4.92 μM by cytopathic effect assay. 7-Hydroxyisoflavone could reduce EV71 viral RNA and protein synthesis in a dose-dependent manner. Time course study showed that treatment of Vero cells with 7-hydroxyisoflavone at indicated times after EV71 inoculation (0-6 h) resulted in significant antiviral activity. Results showed that 7-hydroxyisoflavone acted at an early step of EV71 replication. 7-Hydroxyisoflavone also exhibited strong antiviral activity against coxsackievirus B2, B3, and B6. In short, 7-hydroxyisoflavone may be used as a lead compound for anti-EV71 drug development.
authors: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors: Hui-Qiang
authors: HQ
authors: Wang
authors: Shuai
authors: S
authors: Meng
authors: Zhuo-Rong
authors: ZR
authors: Li
authors: Zong-Gen
authors: ZG
authors: Peng
authors: Yan-Xing
authors: YX
authors: Han
authors: Shan-Shan
authors: SS
authors: Guo
authors: Xiao-Lan
authors: XL
authors: Cui
authors: Yu-Huan
authors: YH
authors: Li
authors: Jian-Dong
authors: JD
authors: Jiang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D004770:Enterovirus / Q000187:drug effects* / Q000235:genetics; D029822:Enterovirus B, Human / Q000187:drug effects*; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D012254:Ribavirin / Q000494:pharmacology; D013997:Time Factors; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: C582365:7-hydroxyisoflavone; D000998:Antiviral Agents; D007529:Isoflavones; D012254:Ribavirin