title: Some characteristics of IL-5-producing T cells in mouse liver induced by Schistosoma japonicum infection.
abstract: Schistosome infection could cause significant liver damage in animal; Th2 cells play an important role in the progress of this disease. In our study, C57BL/6 mice were infected by Schistosoma japonicum and lymphocytes were isolated from the liver to detect some characteristics of interleukin-5 (IL-5)-producing T cells by different methods. The results revealed that S. japonicum infection could induce a large amount of IL-5 in mouse liver T cells by the means of fluorescent bead immunoassay and RT-PCR. Although, mouse liver contained many T cell subsets, such as Th cells, Tc cells, NKT cells, and γδ T cells. Fluorescence activated cell sorting results indicated that Th cells were the main source of IL-5 in the T cell population after phorbol 12-myristate 13-acetate and ionomycin stimulation. Moreover, the percentage of IL-5-producing Th cells continued to increase from 4 to 8 weeks after S. japonicum infection, which differed from the changes of IFN-γ(+) Th1 cells, IL-4(+) Th2 cells, and IL-17A(+) Th17 cells during S. japonicum infection. Additionally, cytokines co-expression results demonstrated that 36.2 % of IL-5(+) Th cells could express IL-4, and 10 % of it could produce IFN-γ or IL-17A. Collectively, these findings implied that IL-5-producing Th cells posses some properties which differ from other cytokines secreting Th cells.
authors: Department of Functional Experiment Centre, Guangzhou Medical University, 510182, Guangzhou, China. xhyhj1977020@sina.com
authors: Hongyan
authors: H
authors: Xie
authors: Dianhui
authors: D
authors: Chen
authors: Xueping
authors: X
authors: Luo
authors: Zhiyan
authors: Z
authors: Gao
authors: Huilong
authors: H
authors: Fang
authors: Jun
authors: J
authors: Huang
mesh_terms: D000818:Animals; D007371:Interferon-gamma / Q000096:biosynthesis; D020381:Interleukin-17 / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis*; D008099:Liver / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D012549:Schistosoma japonicum / Q000276:immunology / Q000472:pathogenicity*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000469:parasitology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D015848:Interleukin-5; D015847:Interleukin-4; D007371:Interferon-gamma