title: Effect of rice cell-derived human granulocyte-macrophage colony-stimulating factor on 5-fluorouracil-induced mucositis in hamsters.
abstract: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an important regulator of the maturation and function of cells in the granulocyte and macrophage lineages, and also plays a significant role in wound healing. In a previous study, we expressed human GM-CSF in rice cells (rice cell-derived human GM-CSF; rhGM-CSF). The purpose of the present study was to evaluate its effect on wound healing in oral mucositis. Oral mucositis was induced in Syrian hamster cheek pouches by 5-fluorouracil treatment and mechanical scratching. Ulcerated areas were treated from days 3 to 14 with an application of 200 µL saline, or of the same volume of a solution containing 0.04, 0.2, or 1 µg/mL rhGM-CSF. Treatment of hamsters with rhGM-CSF reduced the ulcerated areas of the oral mucosa, compared with the control. Early in the healing process, the mucositis tissue layer of the rhGM-CSF-treated group showed significantly decreased myeloperoxidase activity and increased numbers of proliferating cell nuclear antigen (PCNA)-positive cells. Treatment with rhGM-CSF also affected expression of inflammatory cytokines in the ulcerative mucosal tissue. These results demonstrate the efficacy of plant-produced rhGM-CSF in wound healing and have significant implications for the development of rhGM-CSF as a therapeutic agent for ulcerative oral mucositis.
authors: Department of Environmental & Health Chemistry, College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-gu, Seoul 156–756, South Korea.
authors: Jong Hoon
authors: JH
authors: Won
authors: Jung Eun
authors: JE
authors: Ji
authors: Kyong Hoon
authors: KH
authors: Ahn
authors: Seok Kyun
authors: SK
authors: Kim
authors: Jong Min
authors: JM
authors: Choi
authors: Hae Chan
authors: HC
authors: Ha
authors: Hye Mi
authors: HM
authors: Kim
authors: Chang Koo
authors: CK
authors: Yun
authors: Kyuboem
authors: K
authors: Han
authors: Dae Kyong
authors: DK
authors: Kim
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D006224:Cricetinae; D005472:Fluorouracil / Q000633:toxicity*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000235:genetics; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects / Q000201:enzymology; D012275:Oryza / Q000235:genetics; D009195:Peroxidase / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011994:Recombinant Proteins / Q000627:therapeutic use; D013280:Stomatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000964:Antimetabolites, Antineoplastic; D053583:Interleukin-1beta; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D009195:Peroxidase; D005472:Fluorouracil