title: Acute in vivo performance evaluation of the fluorescence affinity sensor in the intravascular and interstitial space in Swine.
abstract: OBJECTIVE
  We assessed and compared the performance levels of a fiber-coupled fluorescence affinity sensor (FAS) for glucose detection in the intradermal tissue and intravascular bed during glucose clamping and insulin administration in a large animal model.
  
  
  METHODS
  The FAS (BioTex Inc., Houston, TX) was implanted in interstitial tissue and in the intravenous space in nondiabetic, anesthetized pigs over 6-7 h. For intradermal assessment, a needle-type FAS was implanted in the upper back using a hypodermic needle. For intravenous assessment, the FAS was inserted through a catheter into the femoral artery and vein. Blood glucose changes were induced by infusion of dextrose and insulin through a catheterized ear or jugular vein.
  
  
  RESULTS
  Based on retrospective analysis, the mean absolute relative error (MARE) of the sensor in blood and interstitial tissue was 11.9% [standard deviation (SD) = ± 9.6%] and 23.8% (SD = ± 19.4%), respectively. When excluding data sets from sensors that were affected by exogenous insulin, the MARE for those sensors tested in interstitial tissue was reduced to 16.3% (SD = ± 12.5%).
  
  
  CONCLUSIONS
  The study demonstrated that the performance level of the FAS device implanted in interstitial tissue and blood can be very high. However, under certain circumstances, exogenous insulin caused the glucose concentration in interstitial tissue to be lower than in blood, which resulted in an overall lower level of accuracy of the FAS device. How significant this physiological effect is in insulin-treated persons with diabetes remains to be seen. In contrast, the level of accuracy of the FAS device in blood was very high because of high mass transfer conditions in blood. While the use of the FAS in both body sites will need further validation, its application in critically ill patients looks particularly promising.
authors: BioTex Inc., Houston, Texas 77054, USA. ralph@biotexmedical.com
authors: Ralph
authors: R
authors: Dutt-Ballerstadt
authors: Colton
authors: C
authors: Evans
authors: Arun P
authors: AP
authors: Pillai
authors: Ashok
authors: A
authors: Gowda
authors: Roger
authors: R
authors: McNichols
authors: Jesse
authors: J
authors: Rios
authors: William
authors: W
authors: Cohn
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D003920:Diabetes Mellitus / Q000097:blood; D005453:Fluorescence; D023421:Models, Animal; D012867:Skin / Q000737:chemistry; D013552:Swine
chemical_list: D001786:Blood Glucose