title: Immune reactivity of Brucella melitensis-vaccinated rabbit serum with recombinant Omp31 and DnaK proteins.
abstract: OBJECTIVE
  Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East.
  
  
  METHODS
  In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein (DnaK) and an outer membrane protein (Omp31) of B. melitensis were cloned, expressed in E.coli BL21 and then purified using Ni-NTA agarose. Immunized serum was prepared from a rabbit inoculated with attenuated B. melitensis.
  
  
  CONCLUSIONS
  It was proved that immunized serum contains antibodies against recombinant Omp31 (rOmp31) and DnaK (rDnaK) by Western blot and ELISA assays. The results may suggest the importance of these proteins as subunit vaccines against B. melitensis as well as targets for immunotherapy.
authors: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran,Iran.
authors: Amir
authors: A
authors: Ghasemi
authors: Mohammad Hossein
authors: MH
authors: Salari
authors: Amir Hassan
authors: AH
authors: Zarnani
authors: Mohammad Reza
authors: MR
authors: Pourmand
authors: Hojat
authors: H
authors: Ahmadi
authors: Abbas
authors: A
authors: Mirshafiey
authors: Mahmood
authors: M
authors: Jeddi-Tehrani
keywords: Brucella; Cloning; ELISA; Immune Reactivity; Protein Expression; Purification