title: Influence of a diet rich in resistant starch on the degradation of non-starch polysaccharides in the large intestine of pigs.
abstract: To investigate the effect of resistant starch to the degradation of other non-starch polysaccharides (NSPs) in the large intestine of pigs, two groups of pigs were fed either a diet containing digestible starch (DS) or a diet containing resistant starch (RS). Both diets contained NSPs from wheat and barley. Digesta from different parts of the large intestine were collected and analysed for sugar composition and carbohydrate-degrading-enzyme activities. Resistant starch, as well as β-glucans and soluble arabinoxylan, was utilised mainly in the caecum. The utilisation of β-glucans and soluble arabinoxylan in the caecum was higher in DS-fed pigs than in RS-fed pigs. Analyses on carbohydrate-degrading-enzyme activities demonstrated that microbial enzyme production was stimulated according to the diet composition, and the enzyme profile throughout the large intestine of RS-fed pigs indicated that the presence of resistant starch shifted the utilisation of NSPs to more distal parts of the colon.
authors: Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703HD Wageningen, The Netherlands.
authors: Melliana C
authors: MC
authors: Jonathan
authors: Daniëlle
authors: D
authors: Haenen
authors: Carol
authors: C
authors: Souza da Silva
authors: Guido
authors: G
authors: Bosch
authors: Henk A
authors: HA
authors: Schols
authors: Harry
authors: H
authors: Gruppen
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D006026:Glycoside Hydrolases / Q000378:metabolism; D001467:Hordeum / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism* / Q000382:microbiology; D054892:Metagenome; D010368:Pectins / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D013213:Starch / Q000378:metabolism*; D013552:Swine; D014908:Triticum / Q000378:metabolism; D014990:Xylans / Q000378:metabolism; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D004040:Dietary Carbohydrates; D020543:Proteome; D014990:Xylans; D047071:beta-Glucans; D010368:Pectins; D013213:Starch; C085118:arabinoxylan; D006026:Glycoside Hydrolases