title: Methylation analysis of tumour suppressor genes in ovarian cancer using MS-MLPA.
abstract: Epigenetic changes are considered to be a           frequent event during tumour development. Hypermethylation           of promoter CpG islands represents an           alternative mechanism for inactivation of tumour           suppressor genes, DNA repair genes, cell cycle regulators           and transcription factors. The aim of this           study was to investigate promoter methylation of           specific genes in ovarian cancer by comparison with           normal ovarian tissue. To search for epigenetic events           we used methylation-specific multiplex ligation-dependent           probe amplification (MS-MLPA) to compare           the methylation status of 69 tissue samples of           ovarian cancer with 40 control samples. Using a 15%           cut-off for methylation, we observed significantly           higher methylation in genes MGMT, PAX5, CDH13,           WT1, THBS1, GATA5 in the ovarian cancer group,           while in the ESR1 gene we observed significantly           higher methylation in the control group compared           with the ovarian cancer group. These findings could           potentially be used in screening of ovarian cancer           and may have implications for future chemotherapy           based on epigenetic changes.
authors: Department of Clinical Biochemistry and Diagnostics, Faculty Hospital, Faculty of Medicine Hradec Králové, Charles University-Prague, Hradec Králové, Czech Republic. ChmelarovaMarcela@seznam.cz
authors: M
authors: M
authors: Chmelařová
authors: E
authors: E
authors: Křepinská
authors: J
authors: J
authors: Spaček
authors: J
authors: J
authors: Laco
authors: J
authors: J
authors: Nekvindová
authors: V
authors: V
authors: Palička
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D052138:Genes, Neoplasm / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D011401:Promoter Regions, Genetic