title: Detection of pancreatic cancer using serum protein profiling.
abstract: BACKGROUND
  Currently, no suitable biomarkers for the early detection of pancreatic cancer (PC) are available. Proteins present in the serum could reflect a state of the disease. In this study, these profiles as a diagnostic marker for PC were evaluated.
  
  
  METHODS
  Serum samples were obtained from PC patients (n = 50 calibration set, n = 39 validation set) and healthy volunteers (n = 110 and n = 75 respectively) according to a uniform standardized collection and processing protocol. For peptide and protein isolation, automated solid-phase extraction (SPE) with Weak Cation Exchange (WCX) magnetic beads (MB) was performed using a 96-channel liquid handling platform. Protein profiles were obtained by mass spectrometry (MS) and evaluated by linear discriminant analysis with double cross-validation.
  
  
  RESULTS
  A discriminating profile for PC has been identified, with a sensitivity of 78% and a specificity of 89% in the calibration set with an area under the curve (AUC) of 90%. These results were validated with a sensitivity of 74% and a specificity of 91% (AUC 90%).
  
  
  CONCLUSIONS
  Serum profiles of healthy controls and PC can be discrimated between. Further research is warranted to evaluate specificity and whether this biosignature can be used for early detection in a high risk population.
authors: Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands.
authors: Berit
authors: B
authors: Velstra
authors: Bert A
authors: BA
authors: Bonsing
authors: Bart J
authors: BJ
authors: Mertens
authors: Yuri E M
authors: YE
authors: van der Burgt
authors: Anouck
authors: A
authors: Huijbers
authors: Hans
authors: H
authors: Vasen
authors: Wilma E
authors: WE
authors: Mesker
authors: André M
authors: AM
authors: Deelder
authors: Rob A E M
authors: RA
authors: Tollenaar
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000175:diagnosis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D011237:Predictive Value of Tests; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D002411:Cation Exchange Resins; D009363:Neoplasm Proteins