title: Non-enzymatic hydrolysis of RNA in workers of the ant Nylanderia pubens.
abstract: During preparation of total RNA from Nylanderia pubens (Forel) (Hymenoptera: Formicidae) workers for use in expression library construction, severe RNA degradation consistently occurred. This degradation was masked by spectrophotometric analysis but clearly evident by microfluidic-based assay. Although not specifically identified, the degrading entity was endogenous and localized to the abdomen (terminal abdominal segments) of adult ants. RNA degradation was not observed in preparations of larvae, non-melanized pupae, or eggs. Various RNase and protease inhibitors had no protective effect. However, the metal chelating agent ethylenediaminetetraacetic acid prevented RNA degradation and provides insight into the occurrence.
authors: USDA-ARS Center for Medical, Agricultural and Veterinary Entomology, 1600 SW 23rd Drive, Gainesville, FL 32604, USA. Steven.Valles@ars.usda.gov
authors: Steven M
authors: SM
authors: Valles
authors: Charles A
authors: CA
authors: Strong
authors: Eileen A
authors: EA
authors: Buss
authors: David H
authors: DH
authors: Oi
mesh_terms: D000818:Animals; D001000:Ants / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005260:Female; D006868:Hydrolysis; D007814:Larva / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D046210:Microfluidic Analytical Techniques; D010063:Ovum / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011679:Pupa / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D013053:Spectrophotometry
chemical_list: D012313:RNA