title: Synthesis of branched polysaccharides with tunable degree of branching.
abstract: An in vitro enzyme-catalyzed tandem reaction using the enzymes phosphorylase b from rabbit muscle and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) to obtain branched polyglucans with tunable degree of branching (2% ÷ 13%) is presented. The tunable degree of branching is obtained by varying the reaction conditions such as pH value, the choice of reducing agent and its concentration and reaction time. Linear amylose is formed by the phosphorylase-catalyzed propagation of glucose-1-phosphate while Dg GBE introduces branching points on the α-(1→6) position by relocating short oligosaccharide chains. Our results show that the best way to obtain different degrees of branching with this set of enzymes is by regulation of the reaction time.
authors: Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
authors: Jelena
authors: J
authors: Ciric
authors: Katja
authors: K
authors: Loos
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000737:chemistry; D000818:Animals; D034301:Deinococcus / Q000201:enzymology; D004789:Enzyme Activation; D005936:Glucans / Q000138:chemical synthesis* / Q000737:chemistry; D005958:Glucosephosphates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000201:enzymology; D010763:Phosphorylase b / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D058105:Polymerization; D011817:Rabbits; D011198:Solanum tuberosum / Q000737:chemistry / Q000201:enzymology; D013997:Time Factors
chemical_list: D005936:Glucans; D005958:Glucosephosphates; D010940:Plant Proteins; C031146:maltoheptaose; C031590:glucose-1-phosphate; D010763:Phosphorylase b; D015061:1,4-alpha-Glucan Branching Enzyme