title: Effect of the combinations between pea proteins and soluble fibres on cholesterolaemia and cholesterol metabolism in rats.
abstract: Many functional foods and dietary supplements have been reported to be beneficial for the management of dyslipidaemia, one of the major risk factors for CVD. Soluble fibres and legume proteins are known to be a safe and practical approach for cholesterol reduction. The present study aimed at investigating the hypocholesterolaemic effect of the combinations of these bioactive vegetable ingredients and their possible effects on the expression of genes regulating cholesterol homeostasis. A total of six groups of twelve rats each were fed, for 28 d, Nath's hypercholesterolaemic diets, differing in protein and fibre sources, being, respectively, casein and cellulose (control), pea proteins and cellulose (pea), casein and oat fibres (oat), casein and apple pectin (pectin), pea proteins and oat fibres (pea+oat) and pea proteins and apple pectin (pea+pectin). Administration of each vegetable-containing diet was associated with lower total cholesterol concentrations compared with the control. The combinations (pea+oat and pea+pectin) were more efficacious than fibres alone in modulating cholesterolaemia ( - 53 and - 54%, respectively, at 28 d; P< 0·005). In rats fed the diets containing oat fibres or apple pectin, alone or in combination with pea proteins, a lower hepatic cholesterol content (P< 0·005) and higher hepatic mRNA concentrations of CYP7A1 and NTCP were found when compared with the control rats (P< 0·05). In summary, the dietary combinations of pea proteins and oat fibres or apple pectin are extremely effective in lowering plasma cholesterol concentrations in rats and affect cellular cholesterol homeostasis by up-regulating genes involved in hepatic cholesterol turnover.
authors: Department of Pharmacological Sciences, Università degli Studi di Milano, via Balzaretti 9, 20133 Milan, Italy.
authors: Cinzia
authors: C
authors: Parolini
authors: Stefano
authors: S
authors: Manzini
authors: Marco
authors: M
authors: Busnelli
authors: Elena
authors: E
authors: Rigamonti
authors: Marta
authors: M
authors: Marchesi
authors: Erika
authors: E
authors: Diani
authors: Cesare R
authors: CR
authors: Sirtori
authors: Giulia
authors: G
authors: Chiesa
mesh_terms: D000818:Animals; D018554:Avena / Q000737:chemistry; D001647:Bile Acids and Salts / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000517:prevention & control; D002364:Caseins / Q000627:therapeutic use; D002482:Cellulose / Q000627:therapeutic use; D002784:Cholesterol / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism*; D050171:Dyslipidemias / Q000178:diet therapy* / Q000378:metabolism*; D006706:Homeostasis; D008099:Liver / Q000378:metabolism; D008297:Male; D027845:Malus / Q000737:chemistry; D018532:Pisum sativum / Q000737:chemistry*; D010368:Pectins / Q000627:therapeutic use; D010940:Plant Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012307:Risk Factors; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D002364:Caseins; D004043:Dietary Fiber; D010940:Plant Proteins; D010368:Pectins; D002482:Cellulose; D002784:Cholesterol