title: Immunohistochemical staining, laser capture microdissection, and filter-aided sample preparation-assisted proteomic analysis of target cell populations within tissue samples.
abstract: An important problem involves isolating subpopulations of cells defined by protein markers in clinical tissue samples for proteomic studies. We describe a method termed Immunohistochemical staining, laser capture microdissection (LCM) and filter-aided sample preparation (FASP)-Assisted Proteomic analysis of Target cell populations within tissue samples (ILFAPT). The principle of ILFAPT is that a target cell population expressing a protein of interest can be lit up by immunohistochemical staining and isolated from tissue sections using LCM for FASP and proteomic analysis. Using this method, we isolated a small population of CD90(+) stem-like cells from glioblastoma multiforme tissue sections and identified 674 high-confidence (false discovery rate < 0.01) proteins from 32 nL of CD90(+) cells by LC-MS/MS using an Orbitrap Elite mass spectrometer. We further quantified the relative abundance of proteins identified from equal volumes of LCM-captured CD90(+) and CD90(-) cells, where 109 differentially expressed proteins were identified. The major group of these differentially expressed proteins was relevant to cell adhesion and cellular movement. This ILFAPT method has demonstrated the ability to provide in-depth proteome analysis of a very small specific cell population within tissues. It can be broadly applied to the study of target cell populations within clinical specimens.
authors: Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109-0656, USA.
authors: Jintang
authors: J
authors: He
authors: Jianhui
authors: J
authors: Zhu
authors: Yashu
authors: Y
authors: Liu
authors: Jing
authors: J
authors: Wu
authors: Song
authors: S
authors: Nie
authors: Jason A
authors: JA
authors: Heth
authors: Karin M
authors: KM
authors: Muraszko
authors: Xing
authors: X
authors: Fan
authors: David M
authors: DM
authors: Lubman
mesh_terms: D001921:Brain / Q000378:metabolism / Q000473:pathology*; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D005353:Fibronectins / Q000032:analysis / Q000378:metabolism; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D061346:Laser Capture Microdissection / Q000379:methods*; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D018800:Thy-1 Antigens / Q000032:analysis / Q000378:metabolism
chemical_list: D005353:Fibronectins; D020543:Proteome; D018800:Thy-1 Antigens