title: In silico identification of common epitopes from pathogenic mycobacteria.
abstract: An in silico study was carried out to identify antigens for their possible collective use as vaccine candidates against diseases caused by different classes of pathogenic mycobacteria with significant clinical relevance. The genome sequences of the relevant causative agents were used in order to search for orthologous genes among them. Bioinformatics tools permitted us to identify several conserved sequences with 100% identity with no possibility of cross-reactivity to the normal flora and human proteins. Nine different proteins were characterized using the strain H37Rv as reference and taking into account their functional category, their in vivo expression and subcellular location. T and B cell epitopes were identified in the selected sequences. Theoretical prediction of population coverage was calculated for individual epitopes as well as their combinations. Several identical sequences, belonging to six proteins containing T and B cell epitopes which are not present in selected microorganisms of the normal microbial flora or in human proteins were obtained.
authors: Finlay Institute, Ave. 27, No. 19805, La Lisa, La Habana, Cuba.
authors: Bárbara
authors: B
authors: de la Caridad Addine Ramírez
authors: Reynel
authors: R
authors: Marrón
authors: Rommel
authors: R
authors: Calero
authors: Mayelin
authors: M
authors: Mirabal
authors: Juan Carlos
authors: JC
authors: Ramírez
authors: María E
authors: ME
authors: Sarmiento
authors: Mohd Nor
authors: MN
authors: Norazmi
authors: Armando
authors: A
authors: Acosta
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D001428:Bacterial Vaccines; D001483:Base Sequence; D019295:Computational Biology; D003198:Computer Simulation; D017124:Conserved Sequence; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D016680:Genome, Bacterial; D006801:Humans; D009161:Mycobacterium / Q000235:genetics / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D000939:Epitopes; C072681:Mycobacterium tuberculosis antigens