title: Dma/RNF8 proteins are evolutionarily conserved E3 ubiquitin ligases that target septins.
abstract: The budding yeast proteins Dma1 and Dma2 are members of the unique FHA-RING domain protein family and are linked to mitotic regulation and septin organization by ill-defined mechanisms. We show that Dma2 has ubiquitin ligase activity, and that septins Shs1 and Cdc11 are likely direct in vivo targets. We further propose that human RNF8, rather than Chfr, is the mammalian Dma homolog. As in yeast, RNF8 localizes to the centrosomes and cell division sites and promotes ubiquitylation of the septin SEPT7, whose depletion increases cell division anomalies. Together, these findings reveal evolutionary and functional conservation of Dma proteins, and suggest that RNF8 maintains genome stability through independent, yet analogous, nuclear and cytoplasmic ubiquitylation activities.
authors: The Gurdon Institute, University of Cambridge, Cambridge, UK.
authors: Richard
authors: R
authors: Chahwan
authors: Serge
authors: S
authors: Gravel
authors: Takahiro
authors: T
authors: Matsusaka
authors: Stephen P
authors: SP
authors: Jackson
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D045744:Cell Line, Tumor; D018385:Centrosome / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D034622:RNA Interference; D034741:RNA, Small Interfering; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D058112:Septins / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D054875:Ubiquitination
chemical_list: C477369:CDC11 protein, S pombe; D018797:Cell Cycle Proteins; C493793:DMA2 protein, S cerevisiae; D004268:DNA-Binding Proteins; C103134:Dma1 protein, S pombe; D034741:RNA, Small Interfering; C486521:RNF8 protein, human; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D044767:Ubiquitin-Protein Ligases; C113158:SEPTIN7 protein, human; D058112:Septins
keywords: Chfr; Dma1; Dma2; E3 ubiquitin ligase; RNF8; cytokinesis; midbody; septin-ring; septins; ubiquitin