title: Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf].
abstract: The effects of iron (Fe) deficiency on the low-molecular-weight organic acid (LMWOA) metabolism have been investigated in Carrizo citrange (CC) [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.] roots. Major LMWOAs found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with Fe deficiency. The activities of several enzymes involved in the LMWOA metabolism were also assessed in roots. In the cytosolic fraction, the activities of malate dehydrogenase (cMDH) and phosphoenolpyruvate carboxylase (PEPC) enzymes were 132 and 100% higher in Fe-deficient conditions, whereas the activity of pyruvate kinase was 31% lower and the activity of malic enzyme (ME) did not change. In the mitochondrial fraction, the activities of fumarase, MDH and citrate synthase enzymes were 158, 117 and 53% higher, respectively, in Fe-deficient extracts when compared with Fe-sufficient controls, whereas no significant differences between treatments were found for aconitase (ACO) activity. The expression of their corresponding genes in roots of Fe-deficient plants was higher than that measured in Fe-sufficient controls, except for ACO and ME. Also, dicarboxylate-tricarboxylate carrier (DTC) expression was significantly increased in Fe-deficient roots. In conclusion, Fe deficiency in CC seedlings causes a reprogramming of the carbon metabolism that involves an increase of anaplerotic fixation of carbon via PEPC and MDH activities in the cytosol and a shift of the Krebs cycle in the mitochondria towards a non-cyclic mode, as previously described in herbaceous species. In this scheme, DTC could play an important role shuttling both malate and reducing equivalents between the cytosol and the mitochondria. As a result of this metabolic switch malate and citrate concentrations in roots, xylem sap and root exudates increase.
authors: Department of Citriculture and Vegetal Production, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain. martinez_mru@gva.es
authors: Mary-Rus
authors: MR
authors: Martínez-Cuenca
authors: Domingo J
authors: DJ
authors: Iglesias
authors: Manuel
authors: M
authors: Talón
authors: Javier
authors: J
authors: Abadía
authors: Ana-Flor
authors: AF
authors: López-Millán
authors: Eduardo
authors: E
authors: Primo-Millo
authors: Francisco
authors: F
authors: Legaz
mesh_terms: D000154:Aconitate Hydratase / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism; D002957:Citrus / Q000737:chemistry / Q000201:enzymology / Q000502:physiology*; D003600:Cytosol / Q000201:enzymology; D007501:Iron / Q000032:analysis; D000090463:Iron Deficiencies; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008928:Mitochondria / Q000201:enzymology; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism; D053147:Plant Exudates / Q000737:chemistry; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry / Q000201:enzymology / Q000502:physiology*; D032104:Poncirus / Q000737:chemistry / Q000201:enzymology / Q000502:physiology*; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D052584:Xylem / Q000201:enzymology / Q000235:genetics / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D008293:Malates; D053147:Plant Exudates; D018749:RNA, Plant; C020723:citrate-binding transport protein; D019343:Citric Acid; D002244:Carbon; C030298:malic acid; D007501:Iron; D008291:Malate Dehydrogenase; D011770:Pyruvate Kinase; D010730:Phosphoenolpyruvate Carboxylase; D000154:Aconitate Hydratase