title: Pro-inflammatory effects and oxidative stress in lung macrophages and epithelial cells induced by ambient particulate matter.
abstract: The objective of this study was to compare the toxicological effects of different source-related ambient PM10 samples in regard to their chemical composition. In this context we investigated airborne PM from different sites in Aachen, Germany. For the toxicological investigation human alveolar epithelial cells (A549) and murine macrophages (RAW264.7) were exposed from 0 to 96 h to increasing PM concentrations (0-100 μg/ml) followed by analyses of cell viability, pro-inflammatory and oxidative stress responses. The chemical analysis of these particles indicated the presence of 21 elements, water-soluble ions and PAHs. The toxicological investigations of the PM10 samples demonstrated a concentration- and time-dependent decrease in cell viability and an increase in pro-inflammatory and oxidative stress markers.
authors: Institute of Hygiene and Environmental Medicine, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Human Technology Centre, RWTH Aachen University, Theaterplatz 14, 52056 Aachen, Germany. Electronic address: sabrina.michael@rwth-aachen.de.
authors: S
authors: S
authors: Michael
authors: M
authors: M
authors: Montag
authors: W
authors: W
authors: Dott
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D005858:Germany; D006801:Humans; D016676:Macrophages, Alveolar / Q000187:drug effects; D051379:Mice; D018384:Oxidative Stress; D052638:Particulate Matter / Q000032:analysis / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D011650:Pulmonary Alveoli / Q000187:drug effects
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons
keywords: A549; Al; Aluminum; Arsenic; As; CAT; COPD; CYP; Ca; Cadmium; Calcium; Catalase; Cd; Chemical composition; Chromium; Chronic Obstructive Pulmonary Disease; Copper; Cr; Cu; Cytochrome; Cytotoxicity; ELISA; Enzyme Linked Immunosorbent Assay; FBS; Fe; Fetal Bovine Serum; GPx; GRc; GSH; GSSG; Glutathione; Glutathione Disulphide; Glutathione Peroxidase; Glutathione Reductase; IL; Inflammatory response; Interleukin; Iron; K; LVS; Lead; Low Volume Sampler; Magnesium; Manganese; Mg; Mn; Murine macrophage cells; NADP(+)/NADPH; NF-(K)B; Na; Ni; Nickel; Nicotinamide Adenine Dinucleotide Phosphate; Nuclear Factor-kappa B; Oxidative stress; PAH; PBS; PM; Particulate Matter; Particulate matter; Pb; Phosphate Buffered Saline; Polycyclic Aromatic Hydrocarbons; Potassium; RAW264.7; RNS; ROS; Reactive Nitrogen Species; Reactive Oxygen Species; SEM; SOD; Scanning electron microscopy; Sodium; Superoxide-Dismutase; TC; TNF-α; Total Carbon; Toxicological effects; Tumor Necrosis Factor-α; Type II human alveolar basal epithelial cells; UFP; Ultrafine Particulate Matter; V; VOC; Vanadium; Volatile Organic Compounds; Zinc; Zn