title: Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis.
abstract: It has been demonstrated that Mycobacterium tuberculosis can utilize hyaluronan (HA) as an alternative carbon resource; however, the gene responsible for HA utilization has not been identified. We overexpressed the soluble product of the Rv0394c gene from M. tuberculosis H37Rv in Escherichia coli and purified it using affinity chromatography and anion exchange chromatography. The hyaluronidase and chondrosulfatase activities of the purified recombinant protein Rv0394c (rRv0394c) were detected and quantitatively measured. Analysis of nucleotide and derived amino acid sequences of the Rv0394c gene revealed that homologs of this gene were conserved in pathogenic mycobacteria, but absent in non-pathogenic mycobacteria. In the current study, we provide novel identification and characterization of a gene encoding hyaluronidase and chondrosulfatase in M. tuberculosis.
authors: Division of Bacterial Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street , Nangang Dist., Harbin 15000, PR China.
authors: Xianglian
authors: X
authors: Yuan
authors: Liping
authors: L
authors: Chen
authors: Xiaoxia
authors: X
authors: Deng
authors: Jun
authors: J
authors: Cao
authors: Shenye
authors: S
authors: Yu
authors: Wang
authors: W
authors: Quankai
authors: Hai
authors: H
authors: Pang
authors: Siguo
authors: S
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D002811:Chondroitinsulfatases / Q000235:genetics*; D004926:Escherichia coli; D005798:Genes, Bacterial; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics / Q000472:pathogenicity; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014774:Virulence / Q000235:genetics
chemical_list: D002811:Chondroitinsulfatases; D006821:Hyaluronoglucosaminidase