title: Identification of candidate biomarkers using the Experion™ automated electrophoresis system in serum samples from ovarian cancer patients.
abstract: Ovarian cancer is the most common cause of disease-related death in women globally. Detection of ovarian cancer using new biomarkers is necessary for early diagnosis. To date, there have been no obvious biomarkers for ovarian cancer detection in the incipient stage. In this study, we discovered potential diagnostic serological biomarkers for ovarian cancer using the Experion™ automated electrophoresis system. Sera from 14 healthy women and 84 ovarian cancer patients at stages I- IV were applied to the Experion to compare the protein expression levels. To examine the protein expression pattern of Experion data, proteins in the samples were resolved using 10 and 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by silver staining. The candidate biomarkers elevated in ovarian cancer were purified and determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. α-2-macroglobulin (173.7 kDa), ceruloplasmin (147 kDa), inter-α-trypsin inhibitor family heavy chain-related protein (126 kDa), C-1 inhibitor (115.2 kDa) and hemoglobin α/β (14.4 kDa were overexpressed in the ovarian cancer sera. This study documents a novel way to measure ovarian cancer or cancer-related proteins for biomarkers using the Experion assay system, which should be easily adaptable for high-throughput diagnosis to establish databases of ovarian cancer for clinical applications.
authors: Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul 130-040, Republic of Korea.
authors: Ju Hee
authors: JH
authors: Kim
authors: Yong-Wan
authors: YW
authors: Kim
authors: In-Wook
authors: IW
authors: Kim
authors: Dong Chun
authors: DC
authors: Park
authors: Yong Wook
authors: YW
authors: Kim
authors: Keun-Ho
authors: KH
authors: Lee
authors: Chun Keun
authors: CK
authors: Jang
authors: Woong Shick
authors: WS
authors: Ahn
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000302:isolation & purification; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D000077216:Carcinoma, Ovarian Epithelial; D016022:Case-Control Studies; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood* / Q000175:diagnosis; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins