title: Zymography methods for visualizing hydrolytic enzymes.
abstract: Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful, but often misinterpreted, tool yielding information on potential hydrolytic activities, enzyme forms and the locations of active enzymes. In this Review, zymography techniques are compared in terms of advantages, limitations and interpretations. With in gel zymography, enzyme forms are visualized according to their molecular weights. Proteolytic activities are localized in tissue sections with in situ zymography. In vivo zymography can pinpoint proteolytic activity to sites in an intact organism. Future development of novel substrate probes and improvement in detection and imaging methods will increase the applicability of zymography for (reverse) degradomics studies.
authors: Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, KU Leuven, Belgium.
authors: Jennifer
authors: J
authors: Vandooren
authors: Nathalie
authors: N
authors: Geurts
authors: Erik
authors: E
authors: Martens
authors: Philippe E
authors: PE
authors: Van den Steen
authors: Ghislain
authors: G
authors: Opdenakker
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D010447:Peptide Hydrolases