title: miR-153 regulates SNAP-25, synaptic transmission, and neuronal development.
abstract: SNAP-25 is a core component of the trimeric SNARE complex mediating vesicle exocytosis during membrane addition for neuronal growth, neuropeptide/growth factor secretion, and neurotransmitter release during synaptic transmission. Here, we report a novel microRNA mechanism of SNAP-25 regulation controlling motor neuron development, neurosecretion, synaptic activity, and movement in zebrafish. Loss of miR-153 causes overexpression of SNAP-25 and consequent hyperactive movement in early zebrafish embryos. Conversely, overexpression of miR-153 causes SNAP-25 down regulation resulting in near complete paralysis, mimicking the effects of treatment with Botulinum neurotoxin. miR-153-dependent changes in synaptic activity at the neuromuscular junction are consistent with the observed movement defects. Underlying the movement defects, perturbation of miR-153 function causes dramatic developmental changes in motor neuron patterning and branching. Together, our results indicate that precise control of SNAP-25 expression by miR-153 is critically important for proper neuronal patterning as well as neurotransmission.
authors: Department of Biological Sciences, Vanderbilt University and Medical School, Nashville, Tennessee, United States of America.
authors: Chunyao
authors: C
authors: Wei
authors: Elizabeth J
authors: EJ
authors: Thatcher
authors: Abigail F
authors: AF
authors: Olena
authors: Diana J
authors: DJ
authors: Cha
authors: Ana L
authors: AL
authors: Perdigoto
authors: Andrew F
authors: AF
authors: Marshall
authors: Bruce D
authors: BD
authors: Carter
authors: Kendal
authors: K
authors: Broadie
authors: James G
authors: JG
authors: Patton
mesh_terms: D000818:Animals; D001483:Base Sequence; D005089:Exocytosis / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009046:Motor Neurons / Q000166:cytology*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology*; D050825:Synaptosomal-Associated Protein 25 / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology
chemical_list: D035683:MicroRNAs; D050825:Synaptosomal-Associated Protein 25; D049452:Green Fluorescent Proteins