title: Enantioselective separation of dansyl-DL-amino acids and some racemates on "click" functionalized native α-cyclodextrin based sub-2 μm columns.
abstract: The current work demonstrates that native α-cyclodextrin, anchored onto sub-2 μm silica particles via "click" reactions and packed into a 5 cm column, was found to be effective for the resolution of 11 pairs of dansyl-DL-amino acids (DAAs) using ultra-high performance liquid chromatography (UHPLC). All DAAs were completely or partially separated on the column and the resolution achieved for 7 pairs of DAAs was significantly greater than 1.5. It was found that the buffer type exerted a profound impact on the separation. The effects of analyte substituents adjacent to the chiral center of analytes as well as operation conditions with respect to the separation efficiency were discussed. Five racemic compounds with single or double rings also got resolved on this short α-CD column to some extent.
authors: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459.
authors: Feng
authors: F
authors: Ai
authors: Yong
authors: Y
authors: Wang
authors: Hui
authors: H
authors: Chen
authors: Yanhui
authors: Y
authors: Yang
authors: Timothy Thatt Yang
authors: TT
authors: Tan
authors: Siu-Choon
authors: SC
authors: Ng
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D057930:Click Chemistry; D003619:Dansyl Compounds / Q000032:analysis*; D013237:Stereoisomerism; D047391:alpha-Cyclodextrins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D003619:Dansyl Compounds; D047391:alpha-Cyclodextrins; C032613:alpha-cyclodextrin