title: The first Toll receptor from the triangle-shell pearl mussel Hyriopsis cumingii.
abstract: Toll receptor was first discovered in Drosophila and has an important function in the innate immunity of invertebrates. In this study, the Toll receptor HcToll1 from Hyriopsis cumingii with a full length of 3810 bp consisting of a 3687 bp ORF that encodes a total of 1228 amino acids protein was selected for further study. The HcToll1 protein consisted of a signal peptide, 17 LRR domains, 2 LRRCT domains, 1 LRRNT domain, 1 TM domain, and 1 TIR domain. Phylogenetic analysis results showed that HcToll1 was clustered in one group together with other mollusca tolls. RT-PCR analysis results showed that HcToll1 was expressed in all tested tissues such as hemocytes, hepatopancreas, gills, and mantle. qRT-PCR analysis results showed that HcToll1 expression was increased by the presence of Escherichia coli, Vibrio anguillarum, Staphyloccocus aureus, and White Spot Syndrome Virus (WSSV). Over-expression of HcTIR could up-regulate expression of drosomycin gene in Drosophila S2 cells. The results of our study indicated that HcToll1 is a functional Toll and it has an important function in the generation of innate immune responses of H. cumingii against microbial challenge.
authors: Jiangsu Key Laboratory for Biodiversity & Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. renqian0402@126.com
authors: Qian
authors: Q
authors: Ren
authors: Xue
authors: X
authors: Zhong
authors: Shao-Wu
authors: SW
authors: Yin
authors: Fang-Yuan
authors: FY
authors: Hao
authors: Kai-Min
authors: KM
authors: Hui
authors: Zhao
authors: Z
authors: Zhang
authors: Chi-Yu
authors: CY
authors: Zhang
authors: Xiao-Qiang
authors: XQ
authors: Yu
authors: Wen
authors: W
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012313:RNA / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000502:physiology; D051194:Toll-Like Receptor 1 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D049876:Unionidae / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D014733:Vibrio / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D051194:Toll-Like Receptor 1; D012313:RNA