title: Reversible protein affinity-labelling using bromomaleimide-based reagents.
abstract: Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will be of general interest in chemical biology and proteomics.
authors: Department of Chemistry, University College London, 20 Gordon Street, London, WC1H OAJ, UK.
authors: Ramiz I
authors: RI
authors: Nathani
authors: Vijay
authors: V
authors: Chudasama
authors: Chris P
authors: CP
authors: Ryan
authors: Paul R
authors: PR
authors: Moody
authors: Rachel E
authors: RE
authors: Morgan
authors: Richard J
authors: RJ
authors: Fitzmaurice
authors: Mark E B
authors: ME
authors: Smith
authors: James R
authors: JR
authors: Baker
authors: Stephen
authors: S
authors: Caddick
mesh_terms: D000345:Affinity Labels / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008301:Maleimides / Q000737:chemistry*; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D019809:Streptavidin / Q000737:chemistry*; D013696:Temperature
chemical_list: D000345:Affinity Labels; D008301:Maleimides; D001710:Biotin; D019809:Streptavidin