title: Expression of death receptor-3 in human breast cancer and its functional effects on breast cancer cells in vitro.
abstract: Death receptor-3 (DR3) plays controversial roles in cancer. Currently, DR3 is known to be a functional receptor of vascular endothelial growth inhibitor (VEGI). The role of DR3 in breast cancer remains unclear. The present study investigated DR3 expression in a clinical cohort of breast cancer patients and its role in breast cancer cells in vitro. The expression of DR3 was examined in a breast cancer cohort using quantitative PCR (Q-PCR) and immunohistochemistry (IHC) in comparison to the patients' data. In vitro function of DR3 was examined through the targeting of this molecule in MCF7 and MDA-MB-231 breast cancer cells using ribozyme transgene technology. Decreased DR3 expression was noted in breast cancer tissues compared to normal tissues and decreased expression of DR3 was generally associated with a poorer prognosis as well as a significantly shorter long-term survival (p=0.038). Targeting of DR3 in vitro in breast cancer cell lines resulted in impaired migratory rates compared to respective control cells. Collectively, these data suggest a complex role for DR3 in breast cancer development and progression.
authors: Metastasis and Angiogenesis Research Group, Institute of Cancer and Genetics, Cardiff University School of Medicine, Cardiff CF14 4XN, UK.
authors: Zhicheng
authors: Z
authors: Ge
authors: Andrew J
authors: AJ
authors: Sanders
authors: Lin
authors: L
authors: Ye
authors: Robert E
authors: RE
authors: Mansel
authors: Wen G
authors: WG
authors: Jiang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D066298:In Vitro Techniques; D061986:MCF-7 Cells; D009367:Neoplasm Staging; D011379:Prognosis; D053219:Receptors, Tumor Necrosis Factor, Member 25 / Q000235:genetics*; D015996:Survival Rate
chemical_list: D053219:Receptors, Tumor Necrosis Factor, Member 25