title: mRNA encoding WAVE-Arp2/3-associated proteins is co-localized with foci of active protein synthesis at the leading edge of MRC5 fibroblasts during cell migration.
abstract: During cell spreading, mammalian cells migrate using lamellipodia formed from a large dense branched actin network which produces the protrusive force required for leading edge advancement. The formation of lamellipodia is a dynamic process and is dependent on a variety of protein cofactors that mediate their local regulation, structural characteristics and dynamics. In the present study, we show that mRNAs encoding some structural and regulatory components of the WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex are localized to the leading edge of the cell and associated with sites of active translation. Furthermore, we demonstrate that steady-state levels of ArpC2 and Rac1 proteins increase at the leading edge during cell spreading, suggesting that localized protein synthesis has a pivotal role in controlling cell spreading and migration.
authors: Department of Biochemistry, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
authors: Mark
authors: M
authors: Willett
authors: Michele
authors: M
authors: Brocard
authors: Hilary J
authors: HJ
authors: Pollard
authors: Simon J
authors: SJ
authors: Morley
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000235:genetics*; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D006801:Humans; D056004:Molecular Dynamics Simulation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D012333:RNA, Messenger; C114864:WASF1 protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family