title: Purinoceptor-mediated, capsaicin-resistant excitatory effect of allyl isothiocyanate on neurons of the guinea-pig small intestine.
abstract: Allyl isothiocyanate (AITC; 200 μM) caused atropine- and tetrodotoxin-sensitive longitudinal muscle contraction on the guinea-pig small intestine. The response was not influenced by hexamethonium, a functional blockade of capsaicin-sensitive neurons or by antagonists acting at TRPV1 or TRPA1, but was abolished by the P2 purinoceptor antagonist PPADS (50 μM). It is concluded that cholinergic motoneurons are activated by a purinergic mechanism in the course of the AITC response, independently of capsaicin-sensitive processes or even TRPA1.
authors: Department of Pharmacology and Pharmacotherapy, University Medical School of Pécs, Pécs, Hungary. Lorand.Bartho@aok.pte.hu
authors: Lorand
authors: L
authors: Bartho
authors: Elin
authors: E
authors: Nordtveit
authors: Veronika
authors: V
authors: Szombati
authors: Rita
authors: R
authors: Benko
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D018678:Cholinergic Agents / Q000008:administration & dosage / Q000009:adverse effects; D006168:Guinea Pigs; D018738:Hexamethonium / Q000494:pharmacology; D007421:Intestine, Small / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D017879:Isothiocyanates / Q000008:administration & dosage* / Q000009:adverse effects*; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000378:metabolism; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D050051:Transient Receptor Potential Channels / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D018678:Cholinergic Agents; D017879:Isothiocyanates; D018048:Receptors, Purinergic P2; D050916:TRPV Cation Channels; D050051:Transient Receptor Potential Channels; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; D018738:Hexamethonium; D013779:Tetrodotoxin; D011732:Pyridoxal Phosphate; D001285:Atropine; C004471:allyl isothiocyanate; D002211:Capsaicin