title: Resveratrol affects protein kinase C activity and promotes apoptosis in human colon carcinoma cells.
abstract: BACKGROUND
  Resveratrol has been reported to have potential chemopreventive and apoptosis-inducing properties in a variety of tumor cell lines.
  
  
  OBJECTIVE
  In this study, to investigate the effects of resveratrol on protein kinase C (PKC) activity and apoptosis in human colon carcinoma cells, we used HT-29 cells and examined the PKCα and ERK1/2 signaling pathways.
  
  
  METHODS
  To test the effects of resveratrol on the growth of HT- 29 cells, the cells were exposed to varying concentrations and assessed with the the MTT cell-viability assay. Fluorescence-activated cell sorter (FACS) analysis was applied to determine the effects of resveratrol on cell apoptosis. Western blotting was performed to determine the protein levels of PKCα and ERK1/2. In inhibition experiments, HT-29 cells were treated with Go?6976 or PD98059 for 30 min, followed by exposure to 200 μM resveratrol for 72 h.
  
  
  RESULTS
  Resveratrol had a significant inhibitory effect on HT-29 cell growth. FACS revealed that resveratrol induced apoptosis. Western blotting showed that e phosphorylation of PKCα and ERK1/2 was significantly increased in response to resveratrol treatment. Pre-treatment with PKCα and ERK1/2 inhibitors (Go?6976 and PD98059) promoted apoptosis.
  
  
  CONCLUSIONS
  Resveratrol has significant anti-proliferative effects on the colon cancer cell line HT-29. The PKC- ERK1/2 signaling pathway can partially mediate resveratrol-induced apoptosis of HT-29 cells.
authors: Department of Anesthesia, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors: Jie-Yu
authors: JY
authors: Fang
authors: Zhi-Hua
authors: ZH
authors: Li
authors: Qiang
authors: Q
authors: Li
authors: Wen-Sheng
authors: WS
authors: Huang
authors: Liang
authors: L
authors: Kang
authors: Jian-Ping
authors: JP
authors: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms; D019073:HT29 Cells; D006801:Humans