title: The immune environment of paediatric solid malignancies: evidence from an immunohistochemical study of clinical cases.
abstract: Childhood malignancies are relatively poorly studied in terms of tumour/host interaction. Using tissue arrays of childhood cancers, we analysed immunohistochemical staining for CD68, CD3 and FOXP3 to evaluate infiltration of myeloid cells, lymphocytes and regulatory T cells. Staining for phosphorylated STAT3 was performed in a subset. The majority of paediatric tumours demonstrated a marked infiltration of CD68+ myeloid cells but, with the exception of neuroblastoma, most showed only sparse infiltration of CD3+/ FOXP3- cells. There was evidence for activation of STAT3 in pPNET (50%), ependymoma (45%) and undifferentiated sarcoma (38%), but it was rarely activated in other tumours.
authors: UCL Institute of Child Health, London, UK. j.anderson@ucl.ac.uk
authors: John R
authors: JR
authors: Apps
authors: Fyeza
authors: F
authors: Hasan
authors: Oliver
authors: O
authors: Campus
authors: Sam
authors: S
authors: Behjati
authors: Thomas S
authors: TS
authors: Jacques
authors: Neil
authors: N
authors: J Sebire
authors: John
authors: J
authors: Anderson
mesh_terms: D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000473:pathology; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D046888:Tissue Array Analysis; D059016:Tumor Microenvironment / Q000276:immunology*