title: Hrq1 functions independently of Sgs1 to preserve genome integrity in Saccharomyces cerevisiae.
abstract: Maintenance of genome stability in eukaryotes involves a number of conserved proteins, including RecQ helicases, which play multiple roles at various steps in homologous recombination and DNA repair pathways. Sgs1 has been described as the only RecQ helicase in lower eukaryotes. However, recent studies revealed the presence of a second RecQ helicase, Hrq1, which is most homologous to human RECQL4. Here we show that hrq1Δ mutation resulted in increased mitotic recombination and spontaneous mutation in Saccharomyces cerevisiae, and sgs1Δ mutation had additive effects on the phenotypes of hrq1Δ. We also observed that the hrq1Δ mutant was sensitive to 4-nitroquinoline 1-oxide and cisplatin, which was not complemented by overexpression of Sgs1. In addition, the hrq1Δ sgs1Δ double mutant displayed synthetic growth defect as well as a shortened chronological life span compared with the respective single mutants. Analysis of the type of age-dependent Can(r) mutations revealed that only point mutations were found in hrq1Δ, whereas significant numbers of gross deletion mutations were found in sgs1Δ. Our results suggest that Hrq1 is involved in recombination and DNA repair pathways in S. cerevisiae independent of Sgs1.
authors: Department of Biological Sciences, College of Natural Science, Inha University, Incheon 402-751, Republic of Korea.
authors: Do-Hee
authors: DH
authors: Choi
authors: Rina
authors: R
authors: Lee
authors: Sung-Hun
authors: SH
authors: Kwon
authors: Sung-Ho
authors: SH
authors: Bae
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000378:metabolism; D002945:Cisplatin / Q000378:metabolism; D004260:DNA Repair; D017353:Gene Deletion; D042822:Genomic Instability; D009153:Mutagens / Q000378:metabolism; D053484:RecQ Helicases / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D009153:Mutagens; D029701:Saccharomyces cerevisiae Proteins; D015112:4-Nitroquinoline-1-oxide; C579040:Hrq1 protein, S cerevisiae; C090472:SGS1 protein, S cerevisiae; D053484:RecQ Helicases; D002945:Cisplatin