title: In house development of (99m)Tc-Rhenium sulfide colloidal nanoparticles for sentinel lymph node detection.
abstract: In this study, rhenium sulfide colloidal nanoparticles were developed as radiopharmaceutical for sentinel lymph node detection. We directly used rhenium sulfide as a starting material for the preparation of colloidal nanoparticles. UV-visible spectrophotometry was used for characterization of in house developed colloidal particles. The size distribution of radioactive particles was studied by using membrane filtration method. The percentage of radiolabeled colloidal nanoparticles was determined by paper chromatography (PC). The study also includes in vitro stability, protein binding in human blood and bioevaluation in a rabbit model. The results indicate that 77.27 ± 3.26 % particles of size less than 20nm (suitable for lymphoscintigraphy) were radiolabeled. (99m)Tc labeled rhenium sulfide labeling efficacy with the radiometal is 98.5 ± 0.5%, which remains considerably stable beyond 5h at room temperature. Furthermore, it was observed that 70.2 ± 1.3% radiolabeled colloid complex showed binding with the blood protein. Bioevaluation results show the remarkable achievement of our radiopharmaceutical. The in house prepared (99m)Tc labeled rhenium sulfide colloidal nanoparticles reached the sentinel node within 15 min of post injection. These results indicate that (99m)Tc labeled rhenium sulfide colloid nanoparticles kit produced by a novel procedure seems of significant potential as a feasible candidate for further development to be used in clinical practice.
authors: Institute of Nuclear Medicine & Oncology, Lahore, Pakistan.
authors: Ume-Kalsoom
authors: UK
authors: Dar
authors: Irfanullah
authors: I
authors: Khan
authors: Muhammad
authors: M
authors: Javed
authors: Muhammad
authors: M
authors: Ali
authors: Syed Waqar
authors: SW
authors: Hyder
authors: Sohail
authors: S
authors: Murad
authors: Jamil
authors: J
authors: Anwar
mesh_terms: D000818:Animals; D004355:Drug Stability; D006801:Humans; D007271:Injections, Intradermal; D007553:Isotope Labeling; D008198:Lymph Nodes / Q000000981:diagnostic imaging* / Q000378:metabolism; D061305:Lymphoscintigraphy / Q000379:methods*; D053758:Nanoparticles; D010316:Particle Size; D011485:Protein Binding; D011817:Rabbits; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D012211:Rhenium / Q000008:administration & dosage / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet; D013671:Technetium Tc 99m Sulfur Colloid / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D013696:Temperature
chemical_list: D019275:Radiopharmaceuticals; C030406:technetium Tc rhenium-sulfur colloid; D013671:Technetium Tc 99m Sulfur Colloid; D012211:Rhenium