title: The effect of α-tocopherol, sweet chestnut wood extract and their combination on oxidative stress in vivo and the oxidative stability of meat in broilers.
abstract: 1. This study examined the effect of α-tocopherol (α-T), sweet chestnut wood extract (SCW) and their combination on oxidative stress in vivo and oxidative stability of meat in broilers given diets rich in PUFA. 2. A total of 60 male broilers were individually caged and divided into 6 groups of 10. The C-PALM group received a diet with 7·5% palm fat and the other 5 groups with 7·5% linseed oil. The linseed oil groups were either un-supplemented (C-LIN) or supplemented with α-T or/and SCW as follows: αT-85 (C-LIN diet + 68 IU vit E as all-rac-α-T/kg), αT-200 (C-LIN diet + 183 IU vit E as all-rac-α-T/kg), SCW (C-LIN diet + 3 g SCW/kg) and αT-SCW (C-LIN diet + 68 IU vit E as all-rac-α-T/kg + 3 g SCW/kg). Different parameters of oxidative stress were measured. 3. Linseed oil induced DNA fragmentation and malondialdehyde (MDA) formation, while α-T reduced both parameters, and SCW reduced the DNA damage. A combination (αT-SCW) also reduced plasma MDA. Larger antioxidant capacity of lipid soluble compounds were recorded in groups αT-85, αT-200 and αT-SCW than in the controls but there were no differences between these groups in antioxidant enzymes and total antioxidant status. A combination (αT-SCW) increased tocopherol concentrations in breast muscle and in comparison to the C-LIN group MDA concentrations were reduced in groups αT-85, αT-200 and αT-SCW. 4. It can be concluded that neither of the α-T concentrations were able to prevent all the negative effects of lipid oxidation in vivo and only high concentrations of α-T improved the stability of meat. With the exception of DNA damage, SCW had no impact on in vivo and in vitro measured markers of oxidative stress but may have a sparing or regenerating effect on α-T.
authors: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Domžale, Slovenia.
authors: M
authors: M
authors: Voljč
authors: A
authors: A
authors: Levart
authors: S
authors: S
authors: Zgur
authors: J
authors: J
authors: Salobir
mesh_terms: D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002645:Chickens; D053938:DNA Fragmentation; D004032:Diet; D019587:Dietary Supplements; D004553:Electric Conductivity; D029962:Fagaceae / Q000737:chemistry; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008043:Linseed Oil / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008460:Meat; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D024505:Tocopherols / Q000097:blood / Q000378:metabolism; D014934:Wood; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010936:Plant Extracts; D010938:Plant Oils; D008315:Malondialdehyde; D008043:Linseed Oil; D024502:alpha-Tocopherol; D024505:Tocopherols