title: Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells.
abstract: In the present study, the antitumor effects of the nor‑Oleanane type triterpene saponin, Bigelovii A, isolated from Salicornia bigelovii Torr, were examined. Bigelovii A was demonstrated to inhibit HL‑60 human acute promyelocytic leukaemia cell growth with an IC50 value of 2.15 µg/ml. In addition, Bigelovii A promoted apoptosis in HL‑60 cells, as shown by apoptotic morphological changes and the hypodiploid cell assay. Apoptotic induction by Bigelovii A was associated with the downregulation of Bcl‑2, the upregulation of Bax and the activation of caspase‑3, as demonstrated by RT‑PCR and western blot analysis. In addition, a lactate dehydrogenase release test indicated that Bigelovii A may exhibit cytotoxic activity by the induction of cell membrane impairment. This study is the first to identify that Bigelovii A exhibits potential antitumor activity and induces marked apoptosis and membrane permeabilisation in HL‑60 cells. Bigelovii A may be a novel candidate for future cancer therapy.
authors: Jiangsu Center for Research and Development of Medicinal Plants, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu, PR China.
authors: Fuqin
authors: F
authors: Guan
authors: Haiting
authors: H
authors: Wang
authors: Yu
authors: Y
authors: Shan
authors: Dongmei
authors: D
authors: Zhang
authors: Youyi
authors: Y
authors: Zhao
authors: Yu
authors: Y
authors: Chen
authors: Qizhi
authors: Q
authors: Wang
authors: Ming
authors: M
authors: Wang
authors: Xu
authors: X
authors: Feng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D006690:Bisbenzimidazole / Q000378:metabolism; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004789:Enzyme Activation / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D012503:Saponins; D014315:Triterpenes; D051028:bcl-2-Associated X Protein; C575123:bigelovii A; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3; D006690:Bisbenzimidazole