title: Full sequence analysis and characterization of a human astrovirus type 1 isolate from South Korea.
abstract: Human astroviruses are recognized as an important cause of infantile gastroenteritis around the world. In South Korea, sporadic cases of HAstV infection have been reported since 2002. However, hitherto, there have been no studies reporting the whole genome sequence of an HAstV isolate from South Korea. Hence, we sequenced and analyzed the entire genome of an HAstV-1 strain (lhar) that was isolated in Seoul, South Korea. The whole-genome sequence analysis revealed 3 open reading frames comprising the whole genome: ORF1a (2,763 bp), ORF1b (1,548 bp), and ORF2 (2,364 bp). The lhar strain showed amino acid identities with 8 other reference strains of 87.6-98.7%, 94.2-98.8%, and 62.6-99.0% in the ORF1a, ORF1b, and ORF2 regions, respectively. The amino acid sequence of the capsid region encoded by ORF2 was compared with a total of 19 HAstV-1 strains and 8 HAstVs reference strains isolated in various countries. This revealed 1 amino acid substitution, at aa412 (Pro → Arg) in ORF2. This study, the first to report the full-length sequence of an HAstV isolated in South Korea, is meaningful in that it can be used as a full-length HAstV sequence standard for future comparison studies. It may also prove useful to the field of public health field by facilitating the diagnosis and the prediction of new emerging variants.
authors: Korea Zoonosis Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors: Sung-Geun
authors: SG
authors: Lee
authors: Lae-Hyung
authors: LH
authors: Kang
authors: Weon-Hwa
authors: WH
authors: Jheong
authors: Mi-Hwa
authors: MH
authors: Oh
authors: Gyu-Cheol
authors: GC
authors: Lee
authors: Sujeong
authors: S
authors: Park
authors: Soon-Young
authors: SY
authors: Paik
mesh_terms: D000595:Amino Acid Sequence; D019350:Astroviridae Infections / Q000821:virology; D016000:Cluster Analysis; D016679:Genome, Viral; D006801:Humans; D001257:Mamastrovirus / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins