title: Identification of cisplatin-resistance associated genes through proteomic analysis of human ovarian cancer cells and a cisplatin-resistant subline.
abstract: Chemoresistance to cancer therapy is a major obstacle to the effective treatment of human cancers with cisplatin (DDP), but the mechanisms of cisplatin-resistance are not clear. In this study, we established a cisplatin- resistant human ovarian cancer cell line (COC1/DDP) and identified differentially expressed proteins related to cisplatin resistance. The proteomic expression profiles in COC1 before and after DDP treatment were examined using 2-dimensional electrophoresis technology. Differentially expressed proteins were identified using matrix- assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and high performance liquid chromatography-electrospray tandem MS (NanoUPLC-ESI-MS/MS). 5 protein spots, for cytokeratin 9, keratin 1, deoxyuridine triphosphatase (dUTPase), aarF domain containing kinase 4 (ADCK 4) and cofilin1, were identified to be significantly changed in COC1/DDP compared with its parental cells. The expression of these five proteins was further validated by quantitative PCR and Western blotting, confirming the results of proteomic analysis. Further research on these proteins may help to identify novel resistant biomarkers or reveal the mechanism of cisplatin-resistance in human ovarian cancers.
authors: Department of Gynecology Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors: Jing
authors: J
authors: Zhou
authors: Yue-Hua
authors: YH
authors: Wei
authors: Mei-Yan
authors: MY
authors: Liao
authors: Yan
authors: Y
authors: Xiong
authors: Jie-Lan
authors: JL
authors: Li
authors: Hong-Bing
authors: HB
authors: Cai
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000627:therapeutic use*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D053556:Keratin-1 / Q000235:genetics; D053534:Keratin-9 / Q000235:genetics; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics; D040901:Proteomics / Q000379:methods
chemical_list: D051339:Actin Depolymerizing Factors; D000970:Antineoplastic Agents; D053556:Keratin-1; D053534:Keratin-9; C586096:COQ8B protein, human; D011494:Protein Kinases; D002945:Cisplatin