title: Determination of Raddeanin A in rat plasma by liquid chromatography-tandem mass spectrometry: application to a pharmacokinetic study.
abstract: A simple, rapid and sensitive LC-MS/MS analysis method was developed and validated for the determination of Raddeanin A (RA) in rat plasma. Protein precipitation with three volumes of methanol as the precipitation reagent was used as the sample preparation method. The analysis process was performed on a Thermo Syncronis C18 column with the mobile phase of methanol-water (containing 5mM ammonium formate, pH 2.2) (85:15, v/v). RA and glycyrrhetinic acid (internal standard) were monitored under negative electrospray ionization in multiple reaction monitoring (MRM) mode. Retention time of RA and IS were 2.1 min and 3.5 min, respectively. The limit of detection was 5 ng/mL and the linear range was 50-50,000 ng/mL. The intra-day and inter-day precision was 1.87-2.94% and 3.25-5.36%, and the intra-day and inter-day accuracy ranged from 5.9% to 10.5% and 5.6% to 11.1%, respectively. The absolute recovery was above 90.3%. The method has been successfully translated to the pharmacokinetic study of RA in rats after intravenous and intraperitoneal administration (0.75 mg/kg).
authors: Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors: Xin
authors: X
authors: Luan
authors: Ying-Yun
authors: YY
authors: Guan
authors: Chao
authors: C
authors: Wang
authors: Mei
authors: M
authors: Zhao
authors: Qin
authors: Q
authors: Lu
authors: Ya-Bin
authors: YB
authors: Tang
authors: Ya-Rong
authors: YR
authors: Liu
authors: De-Hong
authors: DH
authors: Yu
authors: Xiao-Lin
authors: XL
authors: Wang
authors: Hong
authors: H
authors: Qi
authors: Chao
authors: C
authors: Fang
authors: Hong-Zhuan
authors: HZ
authors: Chen
mesh_terms: D031883:Anemone / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012503:Saponins; D009828:Oleanolic Acid