title: Buffer-stable chitosan-polyglutamic acid hybrid nanoparticles for biomedical applications.
abstract: In spite of their attractive features, widespread biomedical applications of CS nanoparticles are yet to be realized due to their poor stability in physiological conditions, such as in buffer system at pH 7.4. Buffer-stable chitosan-based hybrid NPs (HNPs) are reported and characterized. Buffer stability is achieved by introducing polyglutamic acid to chitosan. The effect of PGA to CS molar ratio and crosslinking on HNP integrity, buffer stability, and biodegradability are studied. Preliminary in vitro studies are carried out to evaluate targeted uptake efficiency of folate conjugated HNPs. Successful demonstration of buffer stability and cancer cell targeting by HNPs achieves important milestones for chitosan-based nanoparticle technology.
authors: NanoScience Technology Center, Department of Chemistry, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, Florida 32826, USA.
authors: Astha
authors: A
authors: Malhotra
authors: Xiaolei
authors: X
authors: Zhang
authors: James
authors: J
authors: Turkson
authors: Swadeshmukul
authors: S
authors: Santra
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D020811:Biomedical Technology / Q000379:methods*; D002021:Buffers; D045744:Cell Line, Tumor; D048271:Chitosan / Q000494:pharmacology*; D006801:Humans; D008027:Light; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011099:Polyglutamic Acid / Q000494:pharmacology*; D012542:Scattering, Radiation; D012996:Solutions; D055672:Static Electricity
chemical_list: D001672:Biocompatible Materials; D002021:Buffers; D012996:Solutions; D011099:Polyglutamic Acid; D048271:Chitosan; D009113:Muramidase