title: A RAB family antigen of Angiostrongylus cantonensis induces a Th1-type immune response in vitro and in vivo.
abstract: A cDNA library was constructed from an Angiostrongylus cantonensis young adult and the encoded proteins were expressed in Escherichia coli. One reactive antigen, a RAB-2 protein, was selected using an immunoscreening technique. The expression of the Th1-type cytokine IFN-γ was elicited in mouse splenic cells that were co-cultured with the recombinant RAB-2 protein and in the sera of mice that were immunised with this protein and adjuvant (50 μg at 2-week intervals). In the A. cantonensis-infected groups, the mice were orally infected with 35 infective larvae, and a subset of the infected mice were immunised with the recombinant RAB-2 protein in adjuvant. Serum samples were collected every week for ELISA, and the pathological examinations were performed at 14 days post infection (dpi). An increase in IFN-γ expression was noted in the blood, and the brain sections revealed moderate eosinophilic meningitis in the immunised mice. The RAB-2 antigen of A. cantonensis induced a Th1-type immune response both in vitro and in vivo.
authors: School of Medicine, Fu Jen Catholic University, Taiwan.
authors: Chih-Cheng
authors: CC
authors: Chuang
authors: Chi-Wu
authors: CW
authors: Chen
authors: Kua-Eyre
authors: KE
authors: Su
authors: Yueh-Ting
authors: YT
authors: Tsai
authors: Wen-Yuan
authors: WY
authors: Du
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001700:Biomphalaria; D001921:Brain / Q000469:parasitology / Q000473:pathology; D002478:Cells, Cultured; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics / Q000378:metabolism; D018793:Interleukin-13 / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D015848:Interleukin-5 / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D017206:Strongylida Infections / Q000276:immunology* / Q000469:parasitology; D018417:Th1 Cells / Q000276:immunology*; D020693:rab2 GTP-Binding Protein / Q000235:genetics / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D018793:Interleukin-13; D015848:Interleukin-5; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma; D020693:rab2 GTP-Binding Protein