title: Microarray expression profile of long noncoding RNAs in human osteosarcoma.
abstract: Long noncoding RNAs (lncRNAs) are pervasively transcribed and have a critical role in genome regulation. Alterations in the expression of several lncRNAs have been observed in some types of cancers; however, the contributions of lncRNAs to osteosarcoma remain unknown. Here, we describe the expression profile of lncRNAs in osteosarcomas compared with paired adjacent noncancerous tissue using microarray analysis. In our study, 25,733 lncRNAs were expressed in osteosarcoma; 403 lncRNAs were consistently over-regulated and 798 lncRNAs were consistently under-regulated in all samples analyzed (⩾2.0-fold, p<0.05). Quantitative real-time polymerase chain reaction (PCR) was used to validate six over-regulated and four under-regulated lncRNAs. Bioinformatic analysis (gene ontology analysis, pathway analysis and network analysis) was used for further research. Pathway analysis indicated that 32 pathways corresponded to under-regulated transcripts and that 34 pathways corresponded to over-regulated transcripts (p-value cut-off is 0.05). Our results are the first to reveal differentially expressed lncRNAs in osteosarcoma and suggest that lncRNAs may be novel candidate biomarkers for the diagnosis of osteosarcoma and potential targets for therapy.
authors: Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha 410010, PR China.
authors: Jin-Ping
authors: JP
authors: Li
authors: Li-Hong
authors: LH
authors: Liu
authors: Jie
authors: J
authors: Li
authors: You
authors: Y
authors: Chen
authors: Xiao-Wei
authors: XW
authors: Jiang
authors: Yu-Rong
authors: YR
authors: Ouyang
authors: Yin-Qi
authors: YQ
authors: Liu
authors: Hui
authors: H
authors: Zhong
authors: Hui
authors: H
authors: Li
authors: Tao
authors: T
authors: Xiao
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012516:Osteosarcoma / Q000235:genetics*; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger