title: Studying the protein expression in human B lymphoblastoid cells exposed to 1.8-GHz (GSM) radiofrequency radiation (RFR) with protein microarray.
abstract: In the present study, the protein microarray was used to investigate the protein expression in human B-cell lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation (RFR) at the specific absorption rate (SAR) of 2.0 W/kg for 24 h. The differential expression of 27 proteins was found, which were related to DNA damage repair, apoptosis, oncogenesis, cell cycle and proliferation (ratio >1.5-fold, P<0.05). The results validated with Western blot assay indicated that the expression of RPA32 was significantly down-regulated (P<0.05) while the expression of p73 was significantly up-regulated in RFR exposure group (P<0.05). Because of the crucial roles of those proteins in DNA repair and cell apoptosis, the results of present investigation may explain the biological effects of RFR on DNA damage/repair and cell apoptosis.
authors: Department of Environmental and Occupational Health, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang, PR China.
authors: Chen
authors: C
authors: Zhijian
authors: Li
authors: L
authors: Xiaoxue
authors: Zheng
authors: Z
authors: Wei
authors: Lu
authors: L
authors: Yezhen
authors: Lou
authors: L
authors: Jianlin
authors: Lu
authors: L
authors: Deqiang
authors: Chen
authors: C
authors: Shijie
authors: Jin
authors: J
authors: Lifen
authors: He
authors: H
authors: Jiliang
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000528:radiation effects; D015536:Down-Regulation / Q000528:radiation effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism / Q000528:radiation effects; D009687:Nuclear Proteins / Q000378:metabolism / Q000528:radiation effects; D054448:Precursor Cells, B-Lymphoid / Q000378:metabolism* / Q000528:radiation effects*; D040081:Protein Array Analysis; D011506:Proteins / Q000378:metabolism* / Q000528:radiation effects*; D011846:Radio Waves / Q000009:adverse effects*; D051716:Replication Protein A / Q000378:metabolism / Q000528:radiation effects; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000378:metabolism / Q000528:radiation effects; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D004268:DNA-Binding Proteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D011506:Proteins; D051716:Replication Protein A; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; C516794:RPA2 protein, human