title: Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells.
abstract: The development of molecules specific for M. tuberculosis-infected cells has important implications, as these tools may facilitate understanding of the mechanisms regulating host pathogen interactions in vivo. In addition, development of new tools capable to targeting M. tuberculosis-infected cells may have potential applications to diagnosis, treatment, and prevention of tuberculosis (TB). Due to the lack of CD1b polymorphism, M. tuberculosis lipid-CD1b complexes could be considered as universal tuberculosis infection markers. The aim of the present study was to display on the PIII surface protein of m13 phage, a human αβ single-chain T-cell receptor molecule specific for CD1b:2-stearoyl-3-hydroxyphthioceranoyl-2´-sulfate-α-α´-D-trehalose (Ac₂SGL) which is a complex presented by human cells infected with M. tuberculosis. The results showed the pIII fusion particle was successfully displayed on the phage surface. The study of the recognition of the recombinant phage in ELISA and immunohistochemistry showed the recognition of CD1b:Ac₂SGL complexes and cells in human lung tissue from a tuberculosis patient respectively, suggesting the specific recognition of the lipid-CD1b complex.
authors: Finlay Institute, Havana, Cuba.
authors: Frank
authors: F
authors: Camacho
authors: Jim
authors: J
authors: Huggett
authors: Louise
authors: L
authors: Kim
authors: Juan F
authors: JF
authors: Infante
authors: Marco
authors: M
authors: Lepore
authors: Viviana
authors: V
authors: Perez
authors: María E
authors: ME
authors: Sarmiento
authors: Graham
authors: G
authors: Rook
authors: Armando
authors: A
authors: Acosta
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology*; D018949:Antigens, CD1 / Q000276:immunology*; D017104:Bacteriophage M13; D002460:Cell Line; D061505:Cell Surface Display Techniques; D019673:Genes, T-Cell Receptor alpha; D019674:Genes, T-Cell Receptor beta; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D008168:Lung / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology*; D014764:Viral Proteins
chemical_list: D000942:Antigens, Bacterial; D018949:Antigens, CD1; C419394:CD1b antigen; D006017:Glycolipids; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C011117:sulfoglycolipids