title: Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio.
abstract: Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.
authors: School of Biomedical and Biological Sciences, University of Plymouth, Devon, PL4 8AA, UK.
authors: H C
authors: HC
authors: Reinardy
authors: E
authors: E
authors: Skippins
authors: T B
authors: TB
authors: Henry
authors: A N
authors: AN
authors: Jha
mesh_terms: D000818:Animals; D004249:DNA Damage; D006861:Hydrogen Peroxide / Q000009:adverse effects; D008297:Male; D013076:Sperm Count; D013094:Spermatozoa / Q000473:pathology*; D015027:Zebrafish
chemical_list: D006861:Hydrogen Peroxide