title: Cadinene sesquiterpenes from Eupatorium adenophorum and their antifungal activity.
abstract: Bioactive constituents of Eupatorium adenophorum were investigated for antifungal activity. A structure-antifungal activity relationship of cadinene sesquiterpenes was predicted by evaluating individual derivatives. Cadinene derivatives were extracted from leaves of Eupatorium adenophorum using ethyl acetate. Five cadinene sesquiterpenes were isolated by column chromatography and Preparative Thin Layer Chromatography. Bioactivity of these cadinene sesquiterpenes were evaluated in vitro against four phytopathogenic fungi using poison food technique. Purified sesquiterpenes were spectroscopically elucidated as cadinan-3-ene-2,7-dione (1), 7-hydroxycadinan-3-ene-2-one (2), 5,6-dihydroxycadinan-3-ene-2,7-dione (3), cadinan-3,6-diene-2,7-dione (4) and 2-acetyl-cadinan-3,6-diene-7-one (5). Antifungal evaluation of these compounds against pathogenic fungi was found to be selective. Compound 1 was highly inhibitory towards S. rolfsii (ED50 181.60 ± 0.58 μgmL(-1)) and R. solani (ED50 189.74 ± 1.03 μgmL(-1)). Availability of plant material and significant antifungal activity makes the plant a potential source of antifungal agent and that can be exploited for the development of a natural fungicide.
authors: Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi, India. chem.aditi@gmail.com
authors: Aditi
authors: A
authors: Kundu
authors: Supradip
authors: S
authors: Saha
authors: Suresh
authors: S
authors: Walia
authors: Najam A
authors: NA
authors: Shakil
authors: Jitendra
authors: J
authors: Kumar
authors: Kannepalli
authors: K
authors: Annapurna
mesh_terms: D036603:Ageratina / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects*; D005659:Fungicides, Industrial / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D010935:Plant Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D005659:Fungicides, Industrial; D010936:Plant Extracts; D012717:Sesquiterpenes