title: A prime-boost vaccination of mice with attenuated Salmonella expressing a 30-mer peptide from the Trichinella spiralis gp43 antigen.
abstract: Protection against Trichinella infections has been achieved using various parasite antigens and adjuvants. Recently, we reported that immunization of mice with an attenuated Salmonella strain displaying a 30-mer peptide (residues 210-239) from the Trichinella spiralis gp43 antigen using the ShdA autotransporter induced partial protection against T. spiralis infection. To improve the efficacy of vaccination, we used the MisL autotransporter system to display the Ts30mer peptide on the surface of Salmonella enterica ser. Typhimurium in combination with a prime-boost vaccination strategy. This vector and immunization regimen induced superior protection against T. spiralis when compared to our previously reported approach. Data presented herein showed a significant reduction in adult worm and muscle larvae burdens, high IgG titers, and increased production of intestinal mucus with entrapped adult worms. This prime-boost vaccination scheme is a suitable strategy to elicit enhanced protective immunity against T. spiralis.
authors: Departamento de Genética y Biología Molecular, México, DF, Mexico.
authors: A M
authors: AM
authors: Castillo Alvarez
authors: A
authors: A
authors: Vaquero-Vera
authors: R
authors: R
authors: Fonseca-Liñán
authors: F
authors: F
authors: Ruiz-Pérez
authors: N
authors: N
authors: Villegas-Sepúlveda
authors: G
authors: G
authors: Ortega-Pierres
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D007422:Intestines / Q000469:parasitology; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000469:parasitology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012475:Salmonella / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D017160:Trichinella spiralis / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000517:prevention & control*; D014611:Vaccination
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000951:Antigens, Neoplasm; D011993:Recombinant Fusion Proteins; C107095:tumor-associated antigen, gp43