title: Determination of total folates in infant formula and adult nutritionals by trienzyme extraction and UPLC-MS/MS Quantitation: First Action 2011.06.
abstract: The method for "Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation" was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) to determine the total folates in infant formulas and adult nutritionals after trienzyme digestion. Deconjugation of the various folate polyglutamates to folate monoglutamates is achieved by using rat plasma conjugase after the sample digestion with protease and a-amylase during the trienzyme digestion process. This method shows linearity of folate concentrations in the range of 10-19 100 microg/100 g. Extension of the range to cover folate concentrations of 5-2 000 000 microg/100 g can be achieved with appropriate adjustment of the sample weight and SPE cleanup loading volume. The recoveries ranged from 94.10 to 101.34%.
authors: General Mills/Medallion Laboratories, 9000 Plymouth Ave North, Minneapolis, MN 55427, USA. John.Szpylka@silliker.com
authors: John
authors: J
authors: Szpylka
authors: Jon
authors: J
authors: DeVries
authors: Andrea
authors: A
authors: Cheney
authors: Steve
authors: S
authors: House
mesh_terms: D000465:Algorithms; D000681:Amylases / Q000737:chemistry; D000818:Animals; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000737:chemistry*; D005492:Folic Acid / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D007202:Indicators and Reagents; D007225:Infant Food / Q000032:analysis*; D013058:Mass Spectrometry; D010447:Peptide Hydrolases / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D052616:Solid Phase Extraction; D014815:Vitamins / Q000032:analysis*; D011623:gamma-Glutamyl Hydrolase / Q000097:blood
chemical_list: D002021:Buffers; D004798:Enzymes; D007202:Indicators and Reagents; D014815:Vitamins; D005492:Folic Acid; D000681:Amylases; D010447:Peptide Hydrolases; D011623:gamma-Glutamyl Hydrolase