title: [Expression of aldo-keto reductase family 1 member B10 in gastric cancer tissues and its clinical significance].
abstract: OBJECTIVE
  To investigate the association of AKR1B10 expression in gastric cancer tissues with clinicopathologic features and prognosis of gastric cancer patients.
  
  
  METHODS
  Real-time polymerase chain reaction (RT-PCR) was conducted to detect AKR1B10 mRNA expression in gastric cancer and adjacent gastric mucosa tissues (n=36). AKR1B10 protein expression was measured by immunohistochemistry in primary gastric cancer tissues (n=100) and non-tumorous gastric mucosa tissues (n=70).
  
  
  RESULTS
  RT-PCR results confirmed that AKR1B10 was significantly down-regulated in gastric cancer tissues compared with that in paired adjacent mucosa [8.3% (3/36) vs. 91.7% (33/36), P=0.000]. Immunohistochemistry revealed that the percentage of AKR1B10 positive specimens in gastric carcinoma was lower than that in normal specimens [33.0% (33/100) vs. 92.9% (65/70), P=0.000]. The frequencies of positive AKR1B10 in patients was significantly correlated with tumor size (P=0.000), invasive depth (P=0.004), lymph node metastasis (P=0.028), distant metastasis (P=0.031) and TNM stages (P=0.000). The 5-year survival rate of positive AKR1B10 group was significantly higher as compared to negative group (60.6% vs. 32.8%, P<0.01).
  
  
  CONCLUSIONS
  The down-regulation of AKR1B10 expression in gastric cancer may be associated with the progress of gastric cancer is suggestive of poor prognosis.
authors: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors: Hai-bo
authors: HB
authors: Yao
authors: Yuan
authors: Y
authors: Xu
authors: Le-gao
authors: LG
authors: Chen
authors: Tian-pei
authors: TP
authors: Guan
authors: Ying-Yu
authors: YY
authors: Ma
authors: Hou-quan
authors: HQ
authors: Tao
authors: Qin-shu
authors: QS
authors: Shao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism*; D000074408:Aldo-Keto Reductases; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; C120471:AKR1B10 protein, human; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase