title: Sustained levels of FGF2 maintain undifferentiated stem cell cultures with biweekly feeding.
abstract: An essential aspect of stem cell culture is the successful maintenance of the undifferentiated state. Many types of stem cells are FGF2 dependent, and pluripotent stem cells are maintained by replacing FGF2-containing media daily, while tissue-specific stem cells are typically fed every 3rd day. Frequent feeding, however, results in significant variation in growth factor levels due to FGF2 instability, which limits effective maintenance due to spontaneous differentiation. We report that stabilization of FGF2 levels using controlled release PLGA microspheres improves expression of stem cell markers, increases stem cell numbers and decreases spontaneous differentiation. The controlled release FGF2 additive reduces the frequency of media changes needed to maintain stem cell cultures, so that human embryonic stem cells and induced pluripotent stem cells can be maintained successfully with biweekly feedings.
authors: Neural Stem Cell Institute, Rensselaer, New York, USA.
authors: Steven
authors: S
authors: Lotz
authors: Susan
authors: S
authors: Goderie
authors: Nicolas
authors: N
authors: Tokas
authors: Sarah E
authors: SE
authors: Hirsch
authors: Faizzan
authors: F
authors: Ahmad
authors: Barbara
authors: B
authors: Corneo
authors: Sheila
authors: S
authors: Le
authors: Akhilesh
authors: A
authors: Banerjee
authors: Ravi S
authors: RS
authors: Kane
authors: Jeffrey H
authors: JH
authors: Stern
authors: Sally
authors: S
authors: Temple
authors: Christopher A
authors: CA
authors: Fasano
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D019344:Lactic Acid; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D008863:Microspheres; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology
chemical_list: D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D020928:Mitogen-Activated Protein Kinases