title: Identification of radioresistance-associated proteins in human nasopharyngeal carcinoma cell lines by proteomic analysis.
abstract: OBJECTIVE
  Radiotherapy is the mainstay of treatment modality for human nasopharyngeal carcinoma (NPC), but in some cases, the disease is radioresistant. This study aimed to identify proteins potentially responsible for radioresistance of NPC by a proteomic approach.
  
  
  METHODS
  The radioresistant human NPC cell line CNE-2R and its parental cell line CNE-2 were analyzed by two-dimensional gel electrophoresis and matrix-assisted laser desorption/time-of-flight mass spectrometry analysis. The candidate proteins were validated by Western blot analysis.
  
  
  RESULTS
  In total, 16 differentially expressed proteins were identified; among them, Nm23 H1 was significantly increased, while Annexin A3 was significantly downregulated in CNE-2R cells, compared to CNE-2 cells.
  
  
  CONCLUSIONS
  Our findings provide a platform for further characterization of the proteins implicated in radioresistance and suggest that these proteins could be exploited as a biomarker for predicting the NPC response to radiotherapy in the clinic.
authors: Department of Radiation Oncology, Cancer Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Regional Cancer Hospital, Nanning, PR China.
authors: Ling
authors: L
authors: Li
authors: Shiting
authors: S
authors: Huang
authors: Xiaodong
authors: X
authors: Zhu
authors: Zhirui
authors: Z
authors: Zhou
authors: Yan
authors: Y
authors: Liu
authors: Song
authors: S
authors: Qu
authors: Ya
authors: Y
authors: Guo
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002277:Carcinoma; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D011836:Radiation Tolerance; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome