title: Isolation, identification and antimicrobial activity of propolis-associated fungi.
abstract: Propolis is a natural product widely known for its medicinal properties. In this work, fungi present on propolis samples were isolated, identified and tested for the production of antimicrobial metabolites. Twenty-two fungal isolates were obtained, some of which were identified as Alternaria alternata, Aspergillus flavus, Bipolaris hawaiiensis, Fusarium merismoides, Lasiodiplodia theobromae, Penicillium citrinum, Penicillium crustosum, Penicillium janthinellum, Penicillium purpurogenum, Pestalotiopsis palustris, Tetracoccosporium paxianum and Trichoderma koningii. These fungi were grown in liquid media to obtain crude extracts that were evaluated for their antibiotic activity against pathogenic bacteria, yeast and Cladosporium cladosporioides and A. flavus. The most active extract was obtained from L. theobromae (minimum inhibitory concentration = 64 μg/mL against Listeria monocitogenes). Some extracts showed to be more active than the positive control in the inhibition of Staphylococcus aureus and L. monocitogenes. Therefore, propolis is a promising source of fungi, which produces active agents against relevant food poisoning bacteria and crop-associated fungi.
authors: a Departamento de Química , ICEx, Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
authors: Giovanni Gontijo
authors: GG
authors: de Souza
authors: Ludwig Heinrich
authors: LH
authors: Pfenning
authors: Fabiana
authors: F
authors: de Moura
authors: Mírian
authors: M
authors: Salgado
authors: Jacqueline Aparecida
authors: JA
authors: Takahashi
mesh_terms: D000528:Alternaria / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry; D002963:Cladosporium / Q000187:drug effects; D005658:Fungi / Q000737:chemistry*; D005670:Fusarium / Q000737:chemistry; D010407:Penicillium / Q000737:chemistry; D011429:Propolis / Q000737:chemistry*; D013211:Staphylococcus aureus; D014242:Trichoderma / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D011429:Propolis