title: The effects of Caulerpa microphysa enzyme-digested extracts on ACE-inhibitory activity and in vitro anti-tumour properties.
abstract: The aim of this study was to determine the ACE inhibitory activity and its anti-cancer properties of Caulerpa microphysa extracts. C. microphysa samples were digested with Flavourzyme, Alcalase, and pepsin. The ACE inhibitory activity of enzyme-digested C. microphysa decreased in the order of digestion with pepsin>Flavourzyme>Alcalase; that is, pepsin-extracted samples had significantly higher activity than the other enzyme extractions. To test its anti-tumour effects in vitro C. microphysa pepsin-digested extracts were applied to BALB/c mice with transplanted myelomonocytic leukaemia (WEHI-3) and Human promyelocytic leukaemia (HL-60) cell lines. The growth of both cell lines was inhibited, and extracts induced DNA damage, evaluated with a comet assay. The data demonstrate that C. microphysa pepsin-digested extract had the ability to anti-tumour effects. Further application as a health food is worthy of investigation.
authors: Penghu Marine Biology Research Center, Fisheries Research Institute, COA, EY, No. 8, Xinggang N. St., Magung City, Penghu County 880, Taiwan, ROC.
authors: Hui-Chiu
authors: HC
authors: Lin
authors: Su-Tze
authors: ST
authors: Chou
authors: Mei-Yu
authors: MY
authors: Chuang
authors: Tzu-Yen
authors: TY
authors: Liao
authors: Wann-Sheng
authors: WS
authors: Tsai
authors: Tsai-Hsin
authors: TH
authors: Chiu
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D046068:Caulerpa / Q000737:chemistry*; D045744:Cell Line, Tumor; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004063:Digestion; D010450:Endopeptidases / Q000737:chemistry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D010434:Pepsin A / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012083:Renin / Q000378:metabolism; D013381:Subtilisins / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D010450:Endopeptidases; C412470:flavourzyme; D013381:Subtilisins; D010434:Pepsin A; D012083:Renin