title: Effect of the PA-MSHA vaccine on septic serum-induced inflammatory response.
abstract: Sepsis is defined as a complex clinical syndrome caused by a serious infection followed by an amplified and deregulated inflammatory response. The complex syndrome is associated with a high rate of morbidity and mortality, despite substantial clinical advances. A vaccine derived from the outer membrane proteins of the Gram-negative bacteria Pseudomonas aeruginosa (PA-MSHA) has been demonstrated to exhibit immune modulatory properties. In the present study, the effect of the PA-MSHA vaccine on the inflammatory response induced by serum from septic patients in peripheral blood mononuclear cells was determined. It was observed that PA-MSHA pretreatment inhibits the production of septic serum-induced tumor necrosis factor-α. In addition, PA-MSHA treatment increases interleukin-10 levels and promotes the generation of CD4+CD25+Foxp3+ T cells. Thus, the results of the current study provide mechanistic insight relevant to the potential application of PA-MSHA in the treatment of sepsis.
authors: Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors: Xiang-Feng
authors: XF
authors: Liu
authors: Li
authors: L
authors: Wang
authors: Yi
authors: Y
authors: Qu
authors: De-Wu
authors: DW
authors: Zhong
authors: Xiong-Ying
authors: XY
authors: Miao
authors: Hong-Liang
authors: HL
authors: Yao
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D016032:Randomized Controlled Trials as Topic; D018805:Sepsis / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10