title: Spectroscopic and theoretical study on electronically modified chromophores in LOV domains: 8-bromo- and 8-trifluoromethyl-substituted flavins.
abstract: Two chemically synthesized flavin derivatives, 8-trifluoromethyl- and 8-bromoriboflavin (8-CF(3)RF and 8-BrRF), were photochemically characterized in H(2)O and studied spectroscopically after incorporation into the LOV domain of the blue light photoreceptor YtvA from Bacillus subtilis. The spectroscopic studies were paralleled by high-level quantum chemical calculations. In solution, 8-BrRF showed a remarkably high triplet quantum yield (0.97, parent compound riboflavin, RF: 0.6) and a small fluorescence quantum yield (0.07, RF: 0.27). For 8-CF(3)RF, the triplet yield was 0.12, and the fluorescence quantum yield was 0.7. The high triplet yield of 8-BrRF is due to the bromine heavy atom effect causing a stronger spin-orbit coupling. Theoretical calculations reveal that the decreased triplet yield of 8-CF(3)RF is due to a smaller charge transfer and a less favorable energetic position of T(2), required for intersystem crossing from S(1) to T(1), as an effect of the electron-withdrawing CF(3) group. The reconstitution of the LOV domain with the new flavins resulted in the typical LOV photochemistry, consisting of triplet state formation and covalent binding of the chromophore, followed by a thermal recovery of the parent state, albeit with different kinetics and photophysical properties.
authors: Max Planck Institute for Chemical Energy Conversion, Postfach 10135, 45410 Mülheim an der Ruhr, Germany.
authors: Madina
authors: M
authors: Mansurova
authors: Julian
authors: J
authors: Simon
authors: Susanne
authors: S
authors: Salzmann
authors: Christel M
authors: CM
authors: Marian
authors: Wolfgang
authors: W
authors: Gärtner
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001965:Bromides / Q000737:chemistry; D004583:Electrons; D005415:Flavins / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry; D008962:Models, Theoretical; D020283:Photoreceptors, Microbial / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011789:Quantum Theory; D012256:Riboflavin / Q000031:analogs & derivatives / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D001965:Bromides; D005415:Flavins; D020283:Photoreceptors, Microbial; D005461:Fluorine; C002508:8-bromo-8-demethylriboflavin; D012256:Riboflavin