title: Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme.
abstract: We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in the Gd(III)-nitroxide pulse double electron-electron resonance (DEER or PELDOR) experiment is reported. A modification of the flip angle of the pump pulse in the Gd(III)-nitroxide DEER experiment is proposed to optimize sensitivity.
authors: Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland.
authors: Luca
authors: L
authors: Garbuio
authors: Enrica
authors: E
authors: Bordignon
authors: Evan K
authors: EK
authors: Brooks
authors: Wayne L
authors: WL
authors: Hubbell
authors: Gunnar
authors: G
authors: Jeschke
authors: Maxim
authors: M
authors: Yulikov
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology*; D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D005682:Gadolinium / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009589:Nitrogen Oxides / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D006573:Heterocyclic Compounds, 1-Ring; D009589:Nitrogen Oxides; D013113:Spin Labels; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D005682:Gadolinium; D009113:Muramidase; C039900:nitroxyl