title: In Silico identification of M. TB proteins with diagnostic potential.
abstract: TB, caused by Mycobacterium tuberculosis (MTB), is one of the major global infectious diseases. For the pandemic control, early diagnosis with sensitive and specific methods is fundamental. With the advent of bioinformatics' tools, the identification of several proteins involved in the pathogenesis of TB (TB) has been possible. In the present work, the MTB genome was explored to look for molecules with possible antigenic properties for their evaluation as part of new generation diagnostic kits based on the release of cytokines. Seven proteins from the MTB proteome and some of their combinations suited the computational test and the results suggested their potential use for the diagnosis of infection in the following population groups: Cuba, Mexico, Malaysia and sub-Saharan Africa. Our predictions were performed using public bioinformatics tools plus three computer programs, developed by our group, to facilitate information retrieval and processing.
authors: Finlay Institute, Havana, Cuba. rcalero@finlay.edu.cu
authors: Romel
authors: R
authors: Calero
authors: Mayelin
authors: M
authors: Mirabal
authors: Jesús
authors: J
authors: Bouza
authors: María V
authors: MV
authors: Guzmán
authors: Humberto
authors: H
authors: Carrillo
authors: Yamilé
authors: Y
authors: López
authors: Mohd Nor
authors: MN
authors: Norazmi
authors: Maria E
authors: ME
authors: Sarmiento
authors: Armando
authors: A
authors: Acosta
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D001483:Base Sequence; D019295:Computational Biology; D003198:Computer Simulation; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology*; D020543:Proteome; D017422:Sequence Analysis, DNA; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D020543:Proteome