title: Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish.
abstract: Semaphorin4d (SEMA4D), also known as CD100, an oligodendrocyte secreted R-Ras GTPase-activating protein (GAP), affecting axonal growth is involved in a range of processes including cell adhesion, motility, angiogenesis, immune responses and tumour progression. However, its actual physiological mechanisms and its role in development remain unclear. This study has focused on the role of sema4d in the development and expression patterns in zebrafish embryos and the effect of its suppression on development using sema4d-specific antisense morpholino-oligonucleotides. In this study the knockdown of sema4d, expressed at all developmental stages, lead to defects in the hindbrain and trunk structure of zebrafish embryos. In addition, these phenotypes appeared to be associated with the abnormal expression of three hindbrain rhombomere boundary markers, wnt1, epha4a and foxb1.2, and two myogenic regulatory factors, myod and myog. Further, a notable increase of cell apoptosis appeared in the sema4d knockdown embryos, while no obvious reduction in cell proliferation was observed. Collectively, these data suggest that sema4d plays an important role in the development of the hindbrain and skeletal muscle.
authors: Cancer Biology Research Centre, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China.
authors: Jie
authors: J
authors: Yang
authors: Zhen
authors: Z
authors: Zeng
authors: Juncheng
authors: J
authors: Wei
authors: Lijun
authors: L
authors: Jiang
authors: Quanfu
authors: Q
authors: Ma
authors: Mingfu
authors: M
authors: Wu
authors: Xiaoyuan
authors: X
authors: Huang
authors: Shuangmei
authors: S
authors: Ye
authors: Ye
authors: Y
authors: Li
authors: Ding
authors: D
authors: Ma
authors: Qinglei
authors: Q
authors: Gao
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D018482:Muscle, Skeletal / Q000196:embryology* / Q000473:pathology; D009218:Myosins / Q000235:genetics / Q000378:metabolism; D012249:Rhombencephalon / Q000002:abnormalities / Q000196:embryology* / Q000473:pathology; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; C093224:CD100 antigen; D039961:Semaphorins; D029961:Zebrafish Proteins; D009218:Myosins