title: Flavanols from Japanese quince (Chaenomeles japonica) fruit inhibit human prostate and breast cancer cell line invasiveness and cause favorable changes in Bax/Bcl-2 mRNA ratio.
abstract: Polyphenols are natural compounds of high structural diversity which translates into a very wide spectrum of biological activities, including chemoprevention. Here we report that a Japanese quince fruit flavanol preparation (JQFFP) caused favorable changes in Bax/Bcl-2 mRNA ratio, which rendered normal and cancer cells more resistant and more sensitive, respectively, to apoptosis. DU145 human prostate cancer cells were characterized by the most advantageous Bax/Bcl-2 ratio. The growth and invasiveness of MDA-MB-231 human breast cancer cells were strongly suppressed by JQFFP, which was accompanied with a decrease in MMP-9 activity and stimulation of TIMP-1 expression. Importantly, JQFFP did not decrease normal human prostate PNT1A cell number, whereas Bax/Bcl-2 ratio decreased which implies increased resistance to apoptosis. In conclusion, JQFFP exhibited a potent antiproliferative effect against cancer cells, inhibited their invasiveness, and decreased expression level of several genes involved in apoptosis, angiogenesis, and metastasis.
authors: Department of Medical Enzymology, Medical University of Lodz, Lodz, Poland. urszula.lewandowska@umed.lodz.pl
authors: Urszula
authors: U
authors: Lewandowska
authors: Karolina
authors: K
authors: Szewczyk
authors: Katarzyna
authors: K
authors: Owczarek
authors: Zbigniew
authors: Z
authors: Hrabec
authors: Anna
authors: A
authors: Podsędek
authors: Maria
authors: M
authors: Koziołkiewicz
authors: Elżbieta
authors: E
authors: Hrabec
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000235:genetics; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D027824:Rosaceae / Q000737:chemistry*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; D005419:Flavonoids; D010636:Phenols; D019253:Proto-Oncogene Proteins c-bcl-2; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D051028:bcl-2-Associated X Protein; D020780:Matrix Metalloproteinase 9