title: The "Observer Effect" in genome-wide surveys of protein-RNA interactions.
abstract: Recent technological advances have spurred genome-wide studies that afford insights into ribonucleoprotein biology and transcript regulation on an unprecedented scale. Here we review techniques currently used to obtain genome-wide profiles of RNA-protein interactions in living cells. We highlight recent studies of the mRNA-bound proteome and address pitfalls inherent in such investigations.
authors: Department of Chemistry, Rollins College, Winter Park, FL 32789, USA.
authors: Kasandra J
authors: KJ
authors: Riley
authors: Joan A
authors: JA
authors: Steitz
mesh_terms: D000818:Animals; D016477:Artifacts; D002458:Cell Fractionation; D047369:Chromatin Immunoprecipitation; D016678:Genome; D006801:Humans; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000032:analysis*
chemical_list: D016601:RNA-Binding Proteins