title: The corepressor activity of Alien is controlled by CREB-binding protein/p300.
abstract: The regulation of gene repression by corepressors is a controlled process. Surface-enhanced laser desorption ionization MS proteomic analysis and a yeast two-hybrid screen showed independently that the corepressor Alien interacts with the CREB-binding protein (CBP) coactivator. This interaction was further confirmed by coimmunoprecipitation and glutathione S-transferase pull-down experiments, suggesting that Alien interacts in vivo and in vitro with the histone acetyltransferase (HAT) coactivators CBP and its paralog p300. Acetylation detection experiments indicated that Alien is acetylated in vivo. Furthermore, Alien interacts with the central region of CBP/p300 containing the HAT domain and becomes acetylated in vitro. When an inhibitor of CBP/p300 HAT activity was employed, the Alien-mediated silencing was enhanced. Thus, these findings suggest crosstalk between corepressors and coactivators, and indicate fine-tuning of corepressor function by post-translational modification through corepressor acetylation.
  p300 binds to Alien &#x03B1; by pull down (View interaction) Alien &#x03B1; physically interacts with CBP by two hybrid (View interaction) Alien &#x03B1; physically interacts with MLK2 by two hybrid (View interaction) p300 acetylates Alien &#x03B1; by acetylation assay (View interaction) Alien &#x03B1; physically interacts with NAP1 by two hybrid (View interaction) Alien &#x03B1; physically interacts with TAFI68 by two hybrid (View interaction) Alien &#x03B1; physically interacts with CBP by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3) Alien &#x03B1; binds to CBP by pull down (View interaction).
authors: Institute for Human Genetics, Jena University Hospital, Germany.
authors: Maren
authors: M
authors: Eckey
authors: Florian
authors: F
authors: Kraft
authors: Robert
authors: R
authors: Kob
authors: Niko
authors: N
authors: Escher
authors: Mohammad
authors: M
authors: Asim
authors: Heike
authors: H
authors: Fischer
authors: Marie K
authors: MK
authors: Fritsche
authors: Christian
authors: C
authors: Melle
authors: Aria
authors: A
authors: Baniahmad
mesh_terms: D000107:Acetylation; D000075686:COP9 Signalosome Complex; D050882:CREB-Binding Protein / Q000502:physiology; D056970:Co-Repressor Proteins / Q000502:physiology*; D057809:HEK293 Cells; D051548:Histone Acetyltransferases / Q000378:metabolism; D006801:Humans; D012097:Repressor Proteins / Q000502:physiology*; D050880:p300-CBP Transcription Factors / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: C402762:COPS2 protein, human; D056970:Co-Repressor Proteins; D012097:Repressor Proteins; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; D051548:Histone Acetyltransferases; D050880:p300-CBP Transcription Factors; D000075686:COP9 Signalosome Complex