title: Determination of spinetoram and its metabolites in amaranth and parsley using QuEChERS-based extraction and liquid chromatography-tandem mass spectrometry.
abstract: In this study, a simultaneous method was developed for the determination of spinetoram (XDE-175-J and XDE-175-L) and its demethyl metabolites (N-demethyl-175-J and N-demethyl-175-L) and formyl metabolites (N-formyl-175-J and N-formyl-175-L) in the minor crops; amaranth and parsley. The method uses quick, easy, cheap, effective, rugged, and safe (QuEChERS)-based extraction. Afterwards, the analytes were quantified and confirmed via liquid chromatography-electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) in the positive ion mode using multiple reaction monitoring (MRM). Calibration curves were linear over the calibration ranges for all the analytes tested with r(2)>0.993. Limits of detection and quantitation were 0.01 and 0.03 mg/kg for all the tested analytes in amaranth and parsley, respectively. Recovery values, at spiking levels 0.05 and 0.25 mg/kg, ranged from 71.0% to 115.2% with relative standard deviations <15%, except for N-formyl-175-J in both amaranth and parsley. This method was applied to field-incurred samples and was shown to provide an adequate sensitivity and performance for the simultaneous determination of spinetoram and metabolites. To the best of our knowledge, this is the first time spinetoram and its metabolites were quantified using LC-MS/MS in minor crops.
authors: Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors: Ki Hun
authors: KH
authors: Park
authors: Jeong-Heui
authors: JH
authors: Choi
authors: A M
authors: AM
authors: Abd El-Aty
authors: Soon-Kil
authors: SK
authors: Cho
authors: Jong-Hyouk
authors: JH
authors: Park
authors: Bo Mi
authors: BM
authors: Kim
authors: Angel
authors: A
authors: Yang
authors: Tae Woong
authors: TW
authors: Na
authors: Musfiqur
authors: M
authors: Rahman
authors: Geon-Jae
authors: GJ
authors: Im
authors: Jae-Han
authors: JH
authors: Shim
mesh_terms: D027721:Amaranthus / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007306:Insecticides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018942:Macrolides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D028528:Petroselinum / Q000737:chemistry* / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007306:Insecticides; D018942:Macrolides; C548262:spinetoram