title: In vivo imaging of transplanted islets labeled with a novel cationic nanoparticle.
abstract: To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients.
authors: Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors: Koichi
authors: K
authors: Oishi
authors: Yoshitaka
authors: Y
authors: Miyamoto
authors: Hiroaki
authors: H
authors: Saito
authors: Katsutoshi
authors: K
authors: Murase
authors: Kenji
authors: K
authors: Ono
authors: Makoto
authors: M
authors: Sawada
authors: Masami
authors: M
authors: Watanabe
authors: Yasufumi
authors: Y
authors: Noguchi
authors: Toshiyoshi
authors: T
authors: Fujiwara
authors: Shuji
authors: S
authors: Hayashi
authors: Hirofumi
authors: H
authors: Noguchi
mesh_terms: D000818:Animals; D002412:Cations; D002460:Cell Line; D007515:Islets of Langerhans / Q000033:anatomy & histology*; D016381:Islets of Langerhans Transplantation; D008279:Magnetic Resonance Imaging; D051379:Mice; D053758:Nanoparticles
chemical_list: D002412:Cations