title: Composition of artificial tear solution affects in vitro Pseudomonas aeruginosa biofilm formation on silicone hydrogel lens.
abstract: OBJECTIVE
  To investigate Pseudomonas aeruginosa biofilm formation on silicone hydrogel contact lens in various artificial tears.
  
  
  METHODS
  P. aeruginosa was cocultured with contact lenses (Senofilcon A, Acuvue Oasys Hydraclear plus(®); Johnson & Johnson, Jacksonville, FL) in artificial tears containing hyaluronic acid (HA) (0.3%, 0.1%, or 0.1%+benzalkonium chloride) or carboxymethylcellulose (0.5% or 0.3%+dextran). P. aeruginosa biofilm was stained with crystal violet (0.1%) after 48 h of incubation. The optical density of the dissolved biofilm was measured by a spectrophotometer at 570 nm and was compared.
  
  
  RESULTS
  P. aeruginosa biofilm formation in 3 solutions (0.3% HA, 0.1% HA+benzalkonium chloride and 0.3% carboxymethylcellulose+dextran) was significantly decreased compared to the other 2 solutions (0.1% HA and 0.5% carboxymethylcellulose) (P<0.01).
  
  
  CONCLUSIONS
  P. aeruginosa biofilm formation on silicone hydrogel contact lens was affected by the composition of artificial tears. A higher concentration of HA and addition of benzalkonium chloride or dextran in artificial tears were helpful to prevent P. aeruginosa biofilm formation.
authors: Department of Ophthalmology, Ilsan Hospital, Dongguk University, Koyang, Kyunggido, South Korea.
authors: Mukesh Kumar
authors: MK
authors: Yadav
authors: Roy S
authors: RS
authors: Chuck
authors: Choul Yong
authors: CY
authors: Park
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology; D001548:Benzalkonium Compounds / Q000737:chemistry / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry / Q000494:pharmacology; D003261:Contact Lenses / Q000382:microbiology*; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D009883:Ophthalmic Solutions / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D020034:Silicone Gels
chemical_list: D000891:Anti-Infective Agents, Local; D001548:Benzalkonium Compounds; D003911:Dextrans; D009883:Ophthalmic Solutions; D020034:Silicone Gels; D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium