title: Effect of neuroprotective therapies (hypothermia and cyclosporine a) on dopamine-induced apoptosis in human neuronal SH-SY5Y cells.
abstract: BACKGROUND
  This study aimed to evaluate the effect of hypothermia and CyA on neuronal survival after induced injury in a neuronal model.
  
  
  METHODS
  Human neuroblastoma SH-SY5Y cells were seeded and allowed to grow. To determine whether lower temperatures protect from dopamine-induced apoptosis, cells were treated with dopamine at 100 µM, at 300 µM or without dopamine and incubated at 32 °C or 37 °C for 24 hours. To assess the effect of CyA, cells were pre-incubated with CyA at 37 °C and after dopamine was added.
  
  
  RESULTS
  After 24 hours of incubation at 37 °C, 100 µM and 300 µM dopamine induced 42% (SD = 21) and 58% (SD = 7.9) apoptotic SH-SY5 cells, respectively. In cultures at 32 °C dopamine-induced apoptosis could be reversed by hypothermia [7% (SD = 1.4) and 3.45% (SD = 1.1) for 100 µM and 300 µM, respectively], similar to levels obtained in non-treated cells [2.4% (SD = 1.5)]. Cyclosporine A treatment did not render the expected result, since CyA-pre-treated cells and SH-SY5Y cells showed higher levels of apoptosis than those observed with dopamine alone
  
  
  CONCLUSIONS
  Hypothermia has a marked protective effect against apoptotic cell death induced by dopamine in a human neuroblastic cell line. The neuroprotective effect of CyA described with other apoptotic cell death stimuli was not demonstrated with our experimental conditions.
authors: Department of Critical Care Medicine, University Hospital Marqués de Valdecilla-IFIMAV, Santander, Spain. gelesballesteros@yahoo.es
authors: María A
authors: MA
authors: Ballesteros
authors: María J
authors: MJ
authors: Marín
authors: María San
authors: MS
authors: Martín
authors: María I
authors: MI
authors: Rubio-Lopez
authors: Marcos
authors: M
authors: López-Hoyos
authors: Eduardo
authors: E
authors: Miñambres
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism*; D006801:Humans; D007035:Hypothermia; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D013030:Spain
chemical_list: D018696:Neuroprotective Agents; D016572:Cyclosporine; D004298:Dopamine