title: Optimal place of a foreign gene in the genome of viral haemorrhagic septicaemia virus (VHSV) for development of VHSV-based viral-vectored vaccines.
abstract: OBJECTIVE
  As the strength and duration of immune responses can be regulated by the antigen dose, higher expression of foreign antigens in the viral-vectored vaccines would be an important factor for inducing effective immune responses. The aim of this study was to determine the optimal insertion place of a foreign antigen gene in the genome of viral haemorrhagic septicaemia virus (VHSV) for development of VHSV-based viral-vectored vaccines.
  
  
  RESULTS
  Recombinant VHSVs (rVHSVs) harbouring the red fluorescent protein (RFP) gene between N and P (rVHSV-A-RFP), P and M (rVHSV-B-RFP), or M and G genes (rVHSV-C-RFP) in the genome were rescued by reverse genetics. Their replication ability and expression level of RFP were compared according to the inserted locations. The viral titres of each rVHSV were not significantly different. However, Epithelioma papulosum cyprini (EPC) cells infected with rVHSV-A-RFP or rVHSV-B-RFP showed clearly higher fluorescence than cells infected with rVHSV-C-RFP. There was no significant difference in RFP expression between cells infected with rVHSV-A-RFP and rVHSV-B-RFP.
  
  
  CONCLUSIONS
  The present results indicate that insertion of a foreign gene between N and P, or P and M genes of VHSV genome would be advantageous for development of VHSV-based viral-vectored vaccines.
  
  
  CONCLUSIONS
  The present work is the first report on the optimal location of a foreign gene in VHSV genome for high expression, and the locations identified in this study would be suitable for the development of VHSV-based viral-vectored vaccines.
authors: Department of Aquatic Life Medicine, Pukyong National University, Busan, Korea.
authors: M S
authors: MS
authors: Kim
authors: J S
authors: JS
authors: Park
authors: K H
authors: KH
authors: Kim
mesh_terms: D000941:Antigens / Q000235:genetics; D005822:Genetic Vectors; D016679:Genome, Viral; D008164:Luminescent Proteins / Q000235:genetics; D029141:Novirhabdovirus / Q000235:genetics*; D019444:Vaccines, DNA / Q000235:genetics*; D000097573:Red Fluorescent Protein
chemical_list: D000941:Antigens; D008164:Luminescent Proteins; D019444:Vaccines, DNA; D000097573:Red Fluorescent Protein