title: A rapid Western blotting protocol for the Xenopus oocyte.
abstract: Often experimentalists require a quantitative assessment of the levels of heterologously expressed proteins to best interpret changed Ca(2+) signaling patterns. Here, we detail a rapid and convenient western blotting method for individual Xenopus oocytes. The method exploits recently introduced rapid blotting systems, commercially available from Invitrogen (iBlot) or Bio-Rad (Trans-Blot Turbo). The key advantage is speed: from live cell to transferred membrane in <1 h. Therefore, oocytes can be conveniently processed for western blotting to assess relative expression levels, even after a long day of Ca(2+) imaging experiments.
authors: Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.
authors: Yaping
authors: Y
authors: Lin-Moshier
authors: Jonathan S
authors: JS
authors: Marchant
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D009865:Oocytes / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis*; D014981:Xenopus
chemical_list: D011994:Recombinant Proteins