title: Involvement of Rab28 in NF-κB nuclear transport in endothelial cells.
abstract: Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic strain loading system was used to mimic the mechanical condition on vascular cells during hypertension in vitro. Immunofluorescence and co-immunoprecipitation (Co-IP) were used to identify distribution and interaction of Rab28 and nuclear factor kappa B (NF-κB). Rab28 expression was significantly increased in carotid arteries of hypertensive rats. High cyclic strain induced Rab28 expression of endothelial cells (ECs) through a paracrine control of vascular smooth muscles cells (VSMCs), which at least partly via angiotensin II (Ang II). Rab28 knockdown decreased proliferation of ECs, while increased apoptosis and migration. Immunofluorescence revealed that Ang II stimulated the co-translocation of Rab28 and NF-κB from cytoplasm into nucleus. Knockdown of Rab28 attenuated NF-κB activation. Co-IP of NF-κB p65 and Rab28 indicated their interaction. Our results revealed that Rab28, as a novel regulator of NF-κB nuclear transport, might participate in the disturbance of EC homeostasis.
authors: Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors: Jun
authors: J
authors: Jiang
authors: Ying-Xin
authors: YX
authors: Qi
authors: Ping
authors: P
authors: Zhang
authors: Wen-Tian
authors: WT
authors: Gu
authors: Zhi-Qiang
authors: ZQ
authors: Yan
authors: Bao-Rong
authors: BR
authors: Shen
authors: Qing-Ping
authors: QP
authors: Yao
authors: Han
authors: H
authors: Kong
authors: Shu
authors: S
authors: Chien
authors: Zong-Lai
authors: ZL
authors: Jiang
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D044140:Receptor, Angiotensin, Type 1; C503254:Rab28 protein, rat; D020691:rab GTP-Binding Proteins