title: GC/MS-based metabolomic analysis of cerebrospinal fluid (CSF) from glioma patients.
abstract: Metabolomics has recently undergone rapid development; however, metabolomic analysis in cerebrospinal fluid (CSF) is not a common practice. We analyzed the metabolite profiles of preoperative CSF samples from 32 patients with histologically confirmed glioma using gas chromatography/mass spectrometry (GC/MS). We assessed how alterations in the metabolite levels were related to the World Health Organization (WHO) tumor grades, tumor location, gadolinium enhancement on magnetic resonance imaging (MRI), and the isocitrate dehydrogenase (IDH) mutation status. Sixty-one metabolites were identified in the CSF from glioma patients using targeted, quantitative and non-targeted, semi-quantitative analysis. The citric and isocitric acid levels were significantly higher in the glioblastoma (GBM) samples than in the grades I-II and grade III glioma samples. In addition, the lactic and 2-aminopimelic acid levels were relatively higher in the GBM samples than in the grades I-II glioma samples. The CSF levels of the citric, isocitric, and lactic acids were significantly higher in grade I-III gliomas with mutant IDH than in those with wild-type IDH. The tumor location and enhancement obtained using MRI did not significantly affect the metabolite profiles. Higher CSF levels of lactic acid were statistically associated with a poorer prognosis in grades III-IV malignant gliomas. Our study suggests that the metabolomic analysis of CSF from glioma patients may be useful for predicting the glioma grade, metabolic state, and prognosis of gliomas.
authors: Department of Neurosurgery, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors: Satoshi
authors: S
authors: Nakamizo
authors: Takashi
authors: T
authors: Sasayama
authors: Masakazu
authors: M
authors: Shinohara
authors: Yasuhiro
authors: Y
authors: Irino
authors: Shin
authors: S
authors: Nishiumi
authors: Masamitsu
authors: M
authors: Nishihara
authors: Hirotomo
authors: H
authors: Tanaka
authors: Kazuhiro
authors: K
authors: Tanaka
authors: Katsu
authors: K
authors: Mizukawa
authors: Tomoo
authors: T
authors: Itoh
authors: Masaaki
authors: M
authors: Taniguchi
authors: Kohkichi
authors: K
authors: Hosoda
authors: Masaru
authors: M
authors: Yoshida
authors: Eiji
authors: E
authors: Kohmura
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid* / Q000473:pathology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005910:Glioma / Q000134:cerebrospinal fluid* / Q000473:pathology; D006801:Humans; D008297:Male; D055432:Metabolomics; D008875:Middle Aged; D060787:Neoplasm Grading; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor