title: Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse.
abstract: Identifying the building blocks of mammalian tissues is a precondition for understanding their function. In particular, global and quantitative analysis of the proteome of mammalian tissues would point to tissue-specific mechanisms and place the function of each protein in a whole-organism perspective. We performed proteomic analyses of 28 mouse tissues using high-resolution mass spectrometry and used a mix of mouse tissues labeled via stable isotope labeling with amino acids in cell culture as a "spike-in" internal standard for accurate protein quantification across these tissues. We identified a total of 7,349 proteins and quantified 6,974 of them. Bioinformatic data analysis showed that physiologically related tissues clustered together and that highly expressed proteins represented the characteristic tissue functions. Tissue specialization was reflected prominently in the proteomic profiles and is apparent already in their hundred most abundant proteins. The proportion of strictly tissue-specific proteins appeared to be small. However, even proteins with household functions, such as those in ribosomes and spliceosomes, can have dramatic expression differences among tissues. We describe a computational framework with which to correlate proteome profiles with physiological functions of the tissue. Our data will be useful to the broad scientific community as an initial atlas of protein expression of a mammalian species.
authors: Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
authors: Tamar
authors: T
authors: Geiger
authors: Ana
authors: A
authors: Velic
authors: Boris
authors: B
authors: Macek
authors: Emma
authors: E
authors: Lundberg
authors: Caroline
authors: C
authors: Kampf
authors: Nagarjuna
authors: N
authors: Nagaraj
authors: Mathias
authors: M
authors: Uhlen
authors: Juergen
authors: J
authors: Cox
authors: Matthias
authors: M
authors: Mann
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D015870:Gene Expression; D020869:Gene Expression Profiling; D007553:Isotope Labeling; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D010449:Peptide Mapping; D020543:Proteome / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D000596:Amino Acids; D020543:Proteome