title: The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells.
abstract: NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells.
authors: Department of Advanced Nephrology and Regenerative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors: Osamu
authors: O
authors: Takase
authors: Masahiro
authors: M
authors: Yoshikawa
authors: Mana
authors: M
authors: Idei
authors: Junichi
authors: J
authors: Hirahashi
authors: Toshiro
authors: T
authors: Fujita
authors: Tsuyoshi
authors: T
authors: Takato
authors: Takayuki
authors: T
authors: Isagawa
authors: Genta
authors: G
authors: Nagae
authors: Hirofumi
authors: H
authors: Suemori
authors: Hiroyuki
authors: H
authors: Aburatani
authors: Keiichi
authors: K
authors: Hishikawa
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D015415:Biomarkers; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D016328:NF-kappa B; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D034741:RNA, Small Interfering; D051996:Transcription Factor RelA