title: Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine.
abstract: Tuberculosis (TB) is an air-born, transmissible disease, having an estimated 9.4 million new TB cases worldwide in 2009. Eventual control of this disease by developing a safe and efficient new vaccine able to detain its spread will have an enormous impact on public health policy. Selecting potential antigens to be included in a multi-epitope, minimal subunit-based, chemically-synthesized vaccine containing the minimum sequences needed for blocking mycobacterial interaction with host cells is a complex task due to the multiple mechanisms involved in M. tuberculosis infection and the mycobacterium's immune evasion mechanisms. Our methodology, described here takes into account a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-TB vaccine; it has been based on identifying short mycobacterial protein fragments using synthetic peptides having high affinity interaction with alveolar epithelial cells (A549) and monocyte-derived macrophages (U937) which are able to block the microorganism's entry to target cells in in vitro assays. This manuscript presents a review of the results obtained with some of the MTB H37Rv proteins studied to date, aimed at using these high activity binding peptides (HABPs) as platforms to be included in a minimal subunit-based, multiepitope, chemically-synthesized, antituberculosis vaccine.
authors: Fundación Instituto de Inmunología de Colombia (FIDIC) , Bogotá , Colombia .
authors: Marisol
authors: M
authors: Ocampo
authors: Manuel A
authors: MA
authors: Patarroyo
authors: Magnolia
authors: M
authors: Vanegas
authors: Martha P
authors: MP
authors: Alba
authors: Manuel E
authors: ME
authors: Patarroyo
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D008264:Macrophages / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000276:immunology* / Q000502:physiology; D032581:Tuberculosis Vaccines / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D032581:Tuberculosis Vaccines