title: Description of Xenorhabdus khoisanae sp. nov., the symbiont of the entomopathogenic nematode Steinernema khoisanae.
abstract: Bacterial strain SF87(T), and additional strains SF80, SF362 and 106-C, isolated from the nematode Steinernema khoisanae, are non-bioluminescent Gram-reaction-negative bacteria that share many of the carbohydrate fermentation reactions recorded for the type strains of recognized Xenorhabdus species. Based on 16S rRNA gene sequence data, strain SF87(T) is shown to be closely related (98% similarity) to Xenorhabdus hominickii DSM 17903(T). Nucleotide sequences of strain SF87 obtained from the recA, dnaN, gltX, gyrB and infB genes showed 96-97% similarity with Xenorhabdus miraniensis DSM 17902(T). However, strain SF87 shares only 52.7% DNA-DNA relatedness with the type strain of X. miraniensis, confirming that it belongs to a different species. Strains SF87(T), SF80, SF362 and 106-C are phenotypically similar to X. miraniensis and X. beddingii, except that they do not produce acid from aesculin. These strains are thus considered to represent a novel species of the genus Xenorhabdus, for which the name Xenorhabdus khoisanae sp. nov. is proposed. The type strain is SF87(T) ( =DSM 25463(T) =ATCC BAA-2406(T)).
authors: Department of Conservation Ecology and Entomology, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
authors: Tiarin
authors: T
authors: Ferreira
authors: Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
authors: Carol A
authors: CA
authors: van Reenen
authors: Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
authors: Akihito
authors: A
authors: Endo
authors: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstrasse 7B, 38124 Braunschweig, Germany.
authors: Cathrin
authors: C
authors: Spröer
authors: Department of Conservation Ecology and Entomology, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
authors: Antoinette P
authors: AP
authors: Malan
authors: Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
authors: Leon M T
authors: LMT
authors: Dicks
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017168:Rhabditida / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013019:South Africa; D020593:Xenorhabdus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S