title: Molecular diagnosis of populational variants of Anthonomus grandis (Coleoptera: Curculionidae) in North America.
abstract: The utility of the cytochrome oxidase I (COI) DNA sequence used for DNA barcoding and a Sequence Characterized Amplified Region for diagnosing boll weevil, Anthonomus grandis Boheman, variants was evaluated. Maximum likelihood analysis of COI DNA sequences from 154 weevils collected from the United States and Mexico supports previous evidence for limited gene flow between weevil populations on wild cotton and commercial cotton in northern Mexico and southern United States. The wild cotton populations represent a variant of the species called the thurberia weevil, which is not regarded as a significant pest. The 31 boll weevil COI haplotypes observed in the study form two distinct haplogroups (A and B) that are supported by five fixed nucleotide differences and a phylogenetic analysis. Although wild and commercial cotton populations are closely associated with specific haplogroups, there is not a fixed difference between the thurberia weevil variant and other populations. The Sequence Characterized Amplified Region marker generated a larger number of inconclusive results than the COI gene but also supported evidence of shared genotypes between wild and commercial cotton weevil populations. These methods provide additional markers that can assist in the identification of pest weevil populations but not definitively diagnose samples.
authors: Center for Plant Health Science and Technology, Mission Laboratory, USDA-APHIS, Moore Air Base, 22675 North Moorefield Rd., Edinburg, TX 78541, USA. mexfly@gmail.com
authors: Norman
authors: N
authors: Barr
authors: Raul
authors: R
authors: Ruiz-Arce
authors: Oscar
authors: O
authors: Obregón
authors: Rosita
authors: R
authors: De Leon
authors: Nelson
authors: N
authors: Foster
authors: Chris
authors: C
authors: Reuter
authors: Theodore
authors: T
authors: Boratynski
authors: Don
authors: D
authors: Vacek
mesh_terms: D000818:Animals; D001483:Base Sequence; D058893:DNA Barcoding, Taxonomic; D003576:Electron Transport Complex IV / Q000235:genetics; D050259:Genes, Mitochondrial; D003368:Gossypium; D006239:Haplotypes; D008800:Mexico; D008922:Mississippi; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D015206:Southwestern United States; D048889:Weevils / Q000145:classification* / Q000235:genetics
chemical_list: D003576:Electron Transport Complex IV