title: Detection of genetic diversity in Campylobacter jejuni isolated from a commercial turkey flock using flaA typing, MLST analysis and microarray assay.
abstract: Campylobacter is genetically highly diverse and undergoes frequent intraspecific recombination. Turkeys have been identified as an important reservoir for Campylobacter jejuni which is of public health significance. The assessment of the genetic diversity among Campylobacter population is critical for our understanding of the epidemiology of this bacterium. The genetic profiles were different according to the molecular typing methods used. The performance of established flaA genotyping, multilocus sequencing typing (MLST) and DNA microarray assay based on the ArrayTube™ technology was evaluated using 14 Campylobacter jejuni isolated from a commercial turkey flock. The flaA typing was performed using PCR-RFLP with restriction enzymes Sau3AI, AluI, a 'composite' flaA analysis of AluI and Sau3AI and DdeI. The 14 isolates were differentiated into 3, 5, 7 and 9 genotypes, respectively. Entire flaA gene and short variable region (SVR) sequences were analysed. Sequencing of the entire flaA provided 11 different genotypes. flaA-SVR sequence analysis detected 8 flaA alleles and 4 flaA peptides. One new flaA allele type (528) was identified. MLST analysis represented 10 different sequence types (STs) and 5 clonal complexes (CCs). The microarray assay recognised 14 different genotypes. The discriminatory indices were 0.560, 0.802, 0.857, and 0.912 for flaA-RFLP depending on the used enzymes, 0.890 for flaA-SVR, 0.967 for entire flaA sequencing, 0.945 for MLST and 1.00 for the DNA microarray assay. The flaA gene was genetically stable over 20 passages on blood agar. In conclusion, the different typing tools demonstrated a high level of genetic heterogeneity of Campylobacter jejuni in a turkey flock, indicating that a single flock can be infected by multiple genotypes within one rearing cycle. DNA microarray-based assays had the highest discriminatory power when compared with other genotyping tools.
authors: Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Jena, Germany. hosnyeladawy@yahoo.com
authors: Hosny
authors: H
authors: El-Adawy
authors: Helmut
authors: H
authors: Hotzel
authors: Herbert
authors: H
authors: Tomaso
authors: Heinrich
authors: H
authors: Neubauer
authors: Eduardo N
authors: EN
authors: Taboada
authors: Ralf
authors: R
authors: Ehricht
authors: Hafez M
authors: HM
authors: Hafez
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005408:Flagellin / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D058885:Multilocus Sequence Typing / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D014422:Turkeys / Q000382:microbiology*
chemical_list: D005408:Flagellin; C067138:flaA protein, bacteria