title: Expression of Brachyury gene is a significant prognostic factor for primary lung carcinoma.
abstract: BACKGROUND
  The clinical significance of Brachyury expression and its relationship to epithelial-mesenchymal transition in primary lung carcinoma is unclear.
  
  
  METHODS
  Expression of Brachyury mRNA was investigated in 104 surgically resected primary lung carcinoma tissues. Immunohistochemical analysis of Brachyury transcription factor, Slug, E-cadherin, IL-8, N-cadherin, and Ki67 was performed in 67 of 104 cases, and their expression was correlated to prognoses and clinicopathological factors.
  
  
  RESULTS
  Brachyury mRNA expression in primary lung carcinoma tissues was a significant predictor of poor prognosis for 5-year disease-free survival and overall survival rates and was significantly correlated to vascular invasion, lymphatic permeation, histological grade, pathologic T stage, and pathologic N stage (P < 0.05). Brachyury mRNA expression was significantly inversely correlated to E-cadherin expression (P = 0.0252) and positively correlated to IL-8 protein (P = 0.0241) and to Slug protein (P = 0.0243) in adenocarcinoma tissues.
  
  
  CONCLUSIONS
  A positive association between Brachyury and Slug and IL-8, and a negative association with E-cadherin may lead to invasiveness and metastasis in primary lung carcinoma. Brachyury mRNA expression is a significant predictor of poor prognosis in primary lung carcinoma.
authors: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Kyushu, Japan, aharo@surg2.med.kyushu-u.ac.jp.
authors: Akira
authors: A
authors: Haro
authors: Tokujiro
authors: T
authors: Yano
authors: Mikihiro
authors: M
authors: Kohno
authors: Tsukihisa
authors: T
authors: Yoshida
authors: Takaomi
authors: T
authors: Koga
authors: Tatsuro
authors: T
authors: Okamoto
authors: Mitsuhiro
authors: M
authors: Takenoyama
authors: Yoshihiko
authors: Y
authors: Maehara
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005326:Fetal Proteins / Q000235:genetics* / Q000378:metabolism; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism; D000098908:Brachyury Protein
chemical_list: D014408:Biomarkers, Tumor; D005326:Fetal Proteins; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098908:Brachyury Protein