title: [Analysis of bone tissues by using fluorescent imaging].
abstract: Rapid development of fluorescent imaging techniques enables us to understand cellular dynamics in vivo . We have originally established an advanced imaging system for visualizing live bone tissues with intravital multiphoton microscopy. By means of the system we have recently succeeded in visualization of mature osteoclasts in live bones and revealed that RANKL regulates bone-resorptive functions of mature osteoclasts in vivo . We also developed new image analysis software for tracking morphological changes of mature osteoclasts and generated pH-sensing chemical fluorescent probes for detecting bone resorption at local sites on the bone surface. Here we show the latest data and the detailed methodology of intravital imaging of bone tissues, and also discuss its further application.
authors: Osaka University, Japan Science and Technology, CREST, Japan.
authors: Junichi
authors: J
authors: Kikuta
authors: Masaru
authors: M
authors: Ishii
mesh_terms: D000818:Animals; D001862:Bone Resorption; D001842:Bone and Bones / Q000166:cytology*; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D010010:Osteoclasts / Q000166:cytology*; D053245:RANK Ligand / Q000378:metabolism
chemical_list: D053245:RANK Ligand