title: [Study on the processing technique of enzyme hydrolysis Eupolyphaga sinensis peptide and innunoregulatory effect].
abstract: OBJECTIVE
  Using papain to prepare polypeptide from Eupolyphaga sinensis, then study the immune function of polypeptide from Eupolyphaga sinensis in vivo.
  
  
  METHODS
  Used hydrolysis degree as index, pH value, enzyme dosage, thermometer reaction time were optimized. Studied the influence of polyeptide on the mice immune functions through mice immune organs index, phagocytic function and the level of IL-2.
  
  
  RESULTS
  The optimum enzymolysis condition was as follows: pH 8.0, enzyme 1%, temperature 55 degrees C, reaction time 4. 5 h. In vivo test of mice demonstraed that, Eupolyphaga sinensis could elevate index of thymus and spleen, enhance the phagocytic function of macrophage and promote the level of IL-2 in serum.
  
  
  CONCLUSIONS
  Eupolyphaga sinensis has immunoregulatory effect.
authors: Basic Medical Collage of Jiujiang University, Jiujiang 332000, China.
authors: Dan
authors: D
authors: Liu
authors: Xing-nuan
authors: XN
authors: Li
authors: Zhong-jun
authors: ZJ
authors: Qin
authors: Wei
authors: W
authors: He
authors: Yong
authors: Y
authors: Zhao
mesh_terms: D000818:Animals; D003058:Cockroaches / Q000737:chemistry*; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007376:Interleukin-2 / Q000097:blood; D051379:Mice; D010206:Papain / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
chemical_list: D007376:Interleukin-2; D010455:Peptides; D010206:Papain