title: Flanking sequence determination and event-specific detection of genetically modified wheat B73-6-1.
abstract: In order to establish a specific identification method for genetically modified (GM) wheat, exogenous insert DNA and flanking sequence between exogenous fragment and recombinant chromosome of GM wheat B73-6-1 were successfully acquired by means of conventional polymerase chain reaction (PCR) and thermal asymmetric interlaced (TAIL)-PCR strategies. Newly acquired exogenous fragment covered the full-length sequence of transformed genes such as transformed plasmid and corresponding functional genes including marker uidA, herbicide-resistant bar, ubiquitin promoter, and high-molecular-weight gluten subunit. The flanking sequence between insert DNA revealed high similarity with Triticum turgidum A gene (GenBank: AY494981.1). A specific PCR detection method for GM wheat B73-6-1 was established on the basis of primers designed according to the flanking sequence. This specific PCR method was validated by GM wheat, GM corn, GM soybean, GM rice, and non-GM wheat. The specifically amplified target band was observed only in GM wheat B73-6-1. This method is of high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of GM wheat B73-6-1.
authors: Food Inspection Center, Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China.
authors: Junyi
authors: J
authors: Xu
authors: Jijuan
authors: J
authors: Cao
authors: Dongmei
authors: D
authors: Cao
authors: Tongtong
authors: T
authors: Zhao
authors: Xin
authors: X
authors: Huang
authors: Piqiao
authors: P
authors: Zhang
authors: Fengxia
authors: F
authors: Luan
mesh_terms: D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant