title: The effect of the DLTIDDSYWYRI motif of the human laminin α2 chain on implant osseointegration.
abstract: Considerable effort has been directed towards replacing lost teeth using tissue-engineering methods such as titanium implants. A number of studies have tried to modify bioinert titanium surfaces by coating them with functionally bioactive molecules for faster and stronger osseointegration than pure titanium surfaces. Recently, peptides have been recognized as valuable scientific tools in the field of tissue-engineering. The DLTIDDSYWYRI motif of the human laminin-2 α2 chain has been previously reported to promote the attachment of various cell types; however, the in vivo effects of the DLTIDDSYWYRI motif on new bone formation have not yet been studied. To examine whether a laminin-2-derived peptide can promote osseointegration by accelerating new bone formation in vivo, we applied titanium implants coated with the DLTIDDSYWYRI motif in a rabbit tibia model. The application of the DLTIDDSYWYRI motif-treated implant to tibia wounds enhanced collagen deposition and alkaline phosphatase expression. It significantly promoted implant osseointegration compared with treatment with scrambled peptide-treated implants by increasing the bone-to-implant contact ratio and bone area. These findings support the hypothesis that the DLTIDDSYWYRI motif acts as an effective osseointegration accelerator by enhancing new bone formation.
authors: Department of Oral Biochemistry and Program in Cancer and Developmental Biology, Dental Research Institute, Seoul National University School of Dentistry, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea. Electronic address: kang1978@snu.ac.kr.
authors: Hyun Ki
authors: HK
authors: Kang
authors: Department of Oral Biochemistry and Program in Cancer and Developmental Biology, Dental Research Institute, Seoul National University School of Dentistry, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea. Electronic address: genibok@hanmail.net.
authors: O Bok
authors: OB
authors: Kim
authors: Department of Oral and Maxillofacial Surgery, Seoul National University School of Dentistry, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea. Electronic address: snowmanonmars@gmail.com.
authors: Seung-Ki
authors: SK
authors: Min
authors: Department of Oral Biochemistry and Program in Cancer and Developmental Biology, Dental Research Institute, Seoul National University School of Dentistry, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea. Electronic address: jsy1618@snu.ac.kr.
authors: Sung Youn
authors: SY
authors: Jung
authors: Department of Oral Biochemistry and Program in Cancer and Developmental Biology, Dental Research Institute, Seoul National University School of Dentistry, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea. Electronic address: jdahyun@naver.com.
authors: Da Hyun
authors: DH
authors: Jang
authors: Department of Dentistry, St. Vincent Hospital, Catholic University of Korea, Ji-Dong, Paldal-Ku, Suwon 442-723, Republic of Korea. Electronic address: tega95@naver.com.
authors: Taek-Ka
authors: TK
authors: Kwon
authors: Department of Oral Biochemistry and Program in Cancer and Developmental Biology, Dental Research Institute, Seoul National University School of Dentistry, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea. Electronic address: bmmin@snu.ac.kr.
authors: Byung-Moo
authors: BM
authors: Min
authors: Department of Prosthodontics and Dental Research Institute, Seoul National University School of Dentistry, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea. Electronic address: pros53@snu.ac.kr.
authors: In-Sung
authors: IS
authors: Yeo
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D020007:Implants, Experimental; D007797:Laminin / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D016348:Osseointegration / Q000187:drug effects*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D007797:Laminin; D010455:Peptides; C102645:laminin alpha 2; D003094:Collagen; D014025:Titanium; D000469:Alkaline Phosphatase