title: Phellinus baumii ethyl acetate extract alleviated collagen type II induced arthritis in DBA/1 mice.
abstract: Mushrooms have a long history of dietary benefits in Asia due to their health-promoting effects. Phellinus baumii, a wild mushroom, has been reported to have anti-platelet, anti-inflammatory, anti-obesity and free radical scavenging activities. However, its anti-rheumatoid arthritis (RA) property remains poorly understood. Hence, we investigated the protective effect of Phellinus baumii ethyl acetate extract (PBEAE) against bovine collagen type II induced arthritis (CIA) in DBA/1 mice. PBEAE (50 and 150 mg/kg) reduced the CIA score and leukocyte count in draining lymph nodes (DLNs) and inflamed joints. PBEAE also attenuated the expressions of CD3⁺ (T cells), CD19⁺ (B cells), CD4⁺ (T-helper), CD8⁺ (T-cytotoxic), MHC class II/CD11c⁺ (antigen-presenting cells), double positives (B220⁺/CD23⁺ and CD3⁺/CD69⁺: early lymphocyte activation markers) and CD4⁺/CD25⁺ (activated T-helper) leukocyte subpopulations in DLNs. Likewise, CD3⁺ and Gr-1⁺CD11b⁺ (neutrophil) counts in inflamed joints were also decreased. Furthermore, PBEAE reduced the serum levels of anti-collagen type immunoglobulin G, tumor necrosis factor-α and interleukin (IL)-1β and IL-6. Taken together, PBEAE impaired cellular recruitment to the inflamed joint and alleviated CIA, and thus could be considered as a potential agent against rheumatoid arthritis.
authors: Laboratory of Veterinary Physiology and Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors: Taddesse
authors: T
authors: Yayeh
authors: Whi Min
authors: WM
authors: Lee
authors: Dukhwan
authors: D
authors: Ko
authors: Seung-Choon
authors: SC
authors: Park
authors: Jae Youl
authors: JY
authors: Cho
authors: Hwa-Jin
authors: HJ
authors: Park
authors: In-Kyoung
authors: IK
authors: Lee
authors: Seung-Hyung
authors: SH
authors: Kim
authors: Seung-Bok
authors: SB
authors: Hong
authors: Suk
authors: S
authors: Kim
authors: Bong-Sik
authors: BS
authors: Yun
authors: Man Hee
authors: MH
authors: Rhee
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D001487:Basidiomycota; D002417:Cattle; D002457:Cell Extracts / Q000627:therapeutic use; D024043:Collagen Type II; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000085:Acetates; D000893:Anti-Inflammatory Agents; D002457:Cell Extracts; D024043:Collagen Type II; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C007650:ethyl acetate