title: miR-7641 modulates the expression of CXCL1 during endothelial differentiation derived from human embryonic stem cells.
abstract: MicroRNAs (miRNAs) are a class of small noncoding RNAs that negatively regulate gene expression through binding to 3' untranslated region. We identified and characterized the novel miRNA, miR-7641, in human mesenchymal stem cells. The expression of miR-7641 was downregulated during differentiation from human embryonic stem cells to endothelial cells. The CXCL1, a member of the CXC chemokine family, is known as promoting neovascularization by binding G-protein coupled receptors and is related to endothelial cells biogenesis such as angiogenesis, and it was predicted as target gene of miR-7641 by computerized analysis and the luciferase reporter assay. The miR-7641 significantly suppressed CXCL1 of transcriptional and post-translational levels. These data suggest that miR-7641 might be related with differentiation of human endothelial cells.
authors: Department of Pharmacy, College of Pharmacy, CHA University, Yatap, Bundang, Seongnam, 463-836, Gyeonggi, Republic of Korea.
authors: Jung Ki
authors: JK
authors: Yoo
authors: Ho Yong
authors: HY
authors: Jung
authors: Chang-Hyun
authors: CH
authors: Kim
authors: Woo Sung
authors: WS
authors: Son
authors: Jin Kyeoung
authors: JK
authors: Kim
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D054360:Chemokine CXCL1 / Q000096:biosynthesis* / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data
chemical_list: C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; C585418:MIRN7641 microRNA, human; D035683:MicroRNAs