title: Biosynthesis of 9-methylstreptimidone involves a new decarboxylative step for polyketide terminal diene formation.
abstract: 9-Methylstreptimidone is a glutarimide antibiotic showing antiviral, antifungal, and antitumor activities. Genome scanning, bioinformatics analysis, and gene inactivation experiments reveal a gene cluster responsible for the biosynthesis of 9-methylstreptimidone in Streptomyces himastatinicus. The unveiled machinery features both acyltransferase- and thioesterase-less iterative use of module 5 as well as a branching module for glutarimide generation. Impressively, inactivation of smdK leads to a new carboxylate analogue unveiling a new mechanism for polyketide terminal diene formation.
authors: Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
authors: Bo
authors: B
authors: Wang
authors: Yongxiang
authors: Y
authors: Song
authors: Minghe
authors: M
authors: Luo
authors: Qi
authors: Q
authors: Chen
authors: Junying
authors: J
authors: Ma
authors: Hongbo
authors: H
authors: Huang
authors: Jianhua
authors: J
authors: Ju
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D005810:Multigene Family; D010881:Piperidones / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011090:Polyenes / Q000138:chemical synthesis / Q000737:chemistry*; D061065:Polyketides / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D010881:Piperidones; D011090:Polyenes; D061065:Polyketides; C008497:9-methylstreptimidone; C007864:glutarimide