title: Ni(II)-NTA modified poly(ethylene imine) glycopolymers: physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides.
abstract: Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)-nitrilo(triacetic acid)-modified poly(ethylene imine)-maltose (Ni-NTA-DG) is realized and evaluated as complexation agent against His-tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5-6.0, and also up to 50 mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte-derived immature dendritic cells. In summary, Ni-NTA-DG represents a promising (delivery) platform for forthcoming in vitro applications.
authors: Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, D-01069 Dresden, Germany; Organische Chemie der Polymere, Technische Universität Dresden, D-01062 Dresden, Germany.
authors: Nicole
authors: N
authors: Hauptmann
authors: Marjorie
authors: M
authors: Pion
authors: María-Ángeles
authors: MÁ
authors: Muñoz-Fernández
authors: Hartmut
authors: H
authors: Komber
authors: Carsten
authors: C
authors: Werner
authors: Brigitte
authors: B
authors: Voit
authors: Dietmar
authors: D
authors: Appelhans
mesh_terms: D000595:Amino Acid Sequence; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D055598:Chemical Phenomena; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006678:HIV / Q000737:chemistry*; D006801:Humans; D007097:Imines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D008027:Light; D008320:Maltose / Q000737:chemistry / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009532:Nickel / Q000494:pharmacology*; D009571:Nitrilotriacetic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011095:Polyethylenes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D012542:Scattering, Radiation
chemical_list: D007097:Imines; D010455:Peptides; D011095:Polyethylenes; C505405:poly(ethylene imine); D008320:Maltose; D009532:Nickel; D009571:Nitrilotriacetic Acid