title: Tumor necrosis factor-α gene polymorphism relationship to seminal variables in infertile men.
abstract: OBJECTIVE
  To assess the tumor necrosis factor (TNF)-α gene polymorphism relationship with seminal variables in fertile men (N) and those with asthenozoospermia (A), asthenoteratozoospermia (AT), and oligoasthenoteratozoospermia (OAT).
  
  
  METHODS
  A total of 50 infertile men without a female factor who were attending a fertility clinic and 48 fertile men were randomly screened for semen analysis, analysis of the TNF-α promoter region for polymorphism, seminal caspase-9, acrosin activity, α-glucosidase, and reproductive hormones.
  
  
  RESULTS
  The TNF-α GG genotype was present in 83.9%, 72.7%, 66.7%, and 59.5%, the TNF-α AA genotype in 3.2%, 6.8%, 10.4%, and 11.9%, and TNF-α AG genotype in 12.9%, 20.5%, 22.9%, and 28.6% in the N, A, AT, OAT groups, respectively. The occurrence of A allele was significantly greater among infertile patients than among fertile controls (21.6% vs 9.7%; odds ratio 0.388, 95% confidence interval 0.2 to 0.75, P = .005). Men with the TNF-α AA genotype demonstrated a significant decrease in the sperm count, sperm motility, normal sperm morphology, acrosin activity, and seminal α-glucosidase and a significant increase in seminal caspase-9 compared with those with the TNF-α GG genotype.
  
  
  CONCLUSIONS
  This single nucleotide polymorphism in the TNF-α(-308) gene was associated with significantly increased seminal caspase-9 and a significantly decreased sperm count, sperm motility, normal sperm morphology, acrosin activity, and seminal α-glucosidase.
authors: Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors: Adel
authors: A
authors: Zalata
authors: Amany
authors: A
authors: Atwa
authors: Abd
authors: A
authors: El-Naser Badawy
authors: Amal
authors: A
authors: Aziz
authors: Rizk
authors: R
authors: El-Baz
authors: Samir
authors: S
authors: Elhanbly
authors: Taymour
authors: T
authors: Mostafa
mesh_terms: D005298:Fertility / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D011110:Polymorphism, Genetic; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha