title: In vivo effects of tailored laminin-332 α3 conjugated scaffolds enhances wound healing: a histomorphometric analysis.
abstract: Surface modification techniques have been used to develop biomimetic scaffolds by incorporating cell adhesion peptides. In our previous work, we have shown the tethering of laminin-332 α3 chain to type I collagen scaffold using microbial transglutaminase (mTGase), promotes cell adhesion, migration, and proliferation. In this study, we evaluated the wound healing properties of tailored laminin-332 α3 chain (peptide A: PPFLMLLKGSTR) tethered to a type I collagen scaffold using mTGase by incorporating transglutaminase substrate peptide sequences containing either glutamine (peptide B: PPFLMLLKGSTREAQQIVM) or lysine (peptide C: PPFLMLLKGSTRKKKKG) in rat full-thickness wound model at two different time points (7 and 21 days). Histological evaluations were assessed for wound closure, epithelialization, angiogenesis, inflammatory, fibroblastic cellular infiltrations, and quantified using stereological methods (p < 0.05). Peptide A and B tethered to collagen scaffold using mTGase stimulated neovascularization, decreased the inflammatory cell infiltration and prominently enhanced the fibroblast proliferation which significantly accelerated the wound healing process. We conclude that surface modification by incorporating motif of laminin-332 α3 chain (peptide A: PPFLMLLK GSTR) domain and transglutaminase substrate to the laminin-332 α3 chain (peptide B: PPFLMLLKGSTREAQQIVM) using mTGase may be a potential candidate for tissue engineering applications and skin regeneration.
authors: National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom.
authors: Gopinath
authors: G
authors: Damodaran
authors: William H C
authors: WH
authors: Tiong
authors: Russell
authors: R
authors: Collighan
authors: Martin
authors: M
authors: Griffin
authors: Harshad
authors: H
authors: Navsaria
authors: Abhay
authors: A
authors: Pandit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000494:pharmacology*; D048429:Cell Size / Q000187:drug effects; D024022:Fibrillar Collagens / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*; D011503:Transglutaminases / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D024022:Fibrillar Collagens; D011503:Transglutaminases; D000097794:Kalinin
keywords: collagen; cross-linking; laminin-332 derived peptides; microbial transglutaminase; wound healing