title: Trends in the prevalence of diarrheagenic Escherichia coli among hospitalized diarrheal patients in Kolkata, India.
abstract: BACKGROUND
  To analyse the trends in the prevalence of different pathogroups of diarrheagenic Escherichia coli (DEC) among hospitalized acute diarrheal patients.
  
  
  RESULTS
  From the active surveillance of diarrheal disease at the Infectious Diseases Hospital, Kolkata, 3826 stool specimens collected during 2008-2011 were screened for DEC and other enteric pathogens. PCR was used in the detection of enterotoxigenic, enteropathogenic and enteroaggregative E. coli and 10 major colonization factor antigens (CFs) of enterotoxigenic E. coli. The relationship between DEC infected patient's age group and clinical symptoms were also investigated. Multiplex PCR assay showed that the prevalence of EAEC was most common (5.7%) followed by ETEC (4.2%) and EPEC (1.8%). In diarrheal children >2 year of age, EAEC and EPEC were detected significantly (p = 0.000 and 0.007, respectively). In children >2 to 5 and >5 to 14 years, ETEC was significantly associated with diarrhea (p = 0.000 each). EAEC was significantly associated with diarrheal patients with age groups >14 to 30 and >30 to 50 years (p = 0.001, and p = 0.009, respectively). Clinical symptoms such as vomiting, abdominal pain, watery diarrhea, were recorded in patients infected with ETEC. Dehydration status was severe among patients infected by ST-ETEC (19%) and EPEC (15%). CS6 was frequently detected (37%) among ETEC.
  
  
  CONCLUSIONS
  Hospital based surveillance reviled that specific pathogroups of DEC are important to certain age groups and among ETEC, CS6 was predominant.
authors: Division of Bacteriology, National, Collaborative Research Centre of Okayama University for Infectious Diseases in India, West Bengal, India.
authors: Sanjucta
authors: S
authors: Dutta
authors: Sucharita
authors: S
authors: Guin
authors: Santanu
authors: S
authors: Ghosh
authors: Gururaja P
authors: GP
authors: Pazhani
authors: Krishnan
authors: K
authors: Rajendran
authors: Mihir K
authors: MK
authors: Bhattacharya
authors: Yoshifumi
authors: Y
authors: Takeda
authors: G Balakrish
authors: GB
authors: Nair
authors: Thandavarayan
authors: T
authors: Ramamurthy
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000175:diagnosis* / Q000453:epidemiology*; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000453:epidemiology*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics; D036781:Fimbriae Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D016133:Polymerase Chain Reaction
chemical_list: D029968:Escherichia coli Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins