title: Analysis of proteome profile in germinating soybean seed, and its comparison with rice showing the styles of reserves mobilization in different crops.
abstract: BACKGROUND
  Seed germination is a complex physiological process during which mobilization of nutrient reserves happens. In different crops, this event might be mediated by different regulatory and metabolic pathways. Proteome profiling has been proved to be an efficient way that can help us to construct these pathways. However, no such studies have been performed in soybean germinating seeds up to date.
  
  
  RESULTS
  Proteome profiling was conducted through one-dimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry strategy in the germinating seeds of soybean (glycine max). Comprehensive comparisons were also carried out between rice and soybean germinating seeds. 764 proteins belonging to 14 functional groups were identified and metabolism related proteins were the largest group. Deep analyses of the proteins and pathways showed that lipids were degraded through lipoxygenase dependent pathway and proteins were degraded through both protease and 26S proteosome system, and the lipoxygenase could also help to remove the reactive oxygen species during the rapid mobilization of reserves of soybean germinating seeds. The differences between rice and soybean germinating seeds proteome profiles indicate that each crop species has distinct mechanism for reserves mobilization during germination. Different reserves could be converted into starches before they are totally utilized during the germination in different crops seeds.
  
  
  CONCLUSIONS
  This study is the first comprehensive analysis of proteome profile in germinating soybean seeds to date. The data presented in this paper will improve our understanding of the physiological and biochemical status in the imbibed soybean seeds just prior to germination. Comparison of the protein profile with that of germinating rice seeds gives us new insights on mobilization of nutrient reserves during the germination of crops seeds.
authors: Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.
authors: Chao
authors: C
authors: Han
authors: Xiaojian
authors: X
authors: Yin
authors: Dongli
authors: D
authors: He
authors: Pingfang
authors: P
authors: Yang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism; D018525:Germination; D053858:Metabolic Networks and Pathways; D012275:Oryza / Q000254:growth & development / Q000378:metabolism*; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D013025:Glycine max / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013213:Starch / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D013213:Starch