title: Protective effects of resveratrol on glutamate-induced damages in murine brain cultures.
abstract: Resveratrol interacts with the complex III of the respiratory chain, is a radical scavenger and also suppressor of radical formation in the mitochondria. It reduces the intracellular calcium levels in pre- and postsynaptic neurons and also may inhibit the pro-apoptotic factors in glutamate overflow that occurs, e.g. in excitotoxicity. In cell cultures, glutamate overflow leads to formation of free radicals and results in apoptosis. This increase of radical concentration is enhanced by influx of cations like iron or copper ions into the cell. In present study, the beneficial action of resveratrol was investigated in glutamate-affected dissociated cultures of mice mesencephalic primary cultures. On the 10th day in vitro, 5 mM of glutamate was administered for 15 min and the cultures were further maintained in medium containing 0, 0.01, 0.1 or 1 μM of resveratrol. Resveratrol reduced glutamate-induced damages. The number of dopaminergic neurons was increased and their morphology ameliorated when resveratrol followed glutamate treatment. A significant reduction of glutamate-induced radical formation in cultures treated with resveratrol corresponded with a considerable high antioxidative potential of this stilbene determined using the DPPH assay. In addition, ICP-OES was set up to measure the tissues' copper and iron contents in organotypic cortical cultures of glutamate treated (0 or 30 μM) slices and those in which resveratrol (0, 0.01, 0.1 or 1 μM) was co-administered. Levels of copper were dose-dependently increased, and also the concentration of iron was higher in resveratrol-treated organotypic cultures. The hypothesis that resveratrol has beneficial actions against glutamate damages was verified.
authors: Institute for Medical Biochemistry, University for Veterinary Medicine, Veterinaerplatz 1, 1210, Vienna, Austria. rudolf.moldzio@vu-wien.ac.at
authors: Rudolf
authors: R
authors: Moldzio
authors: Khaled
authors: K
authors: Radad
authors: Christopher
authors: C
authors: Krewenka
authors: Barbara
authors: B
authors: Kranner
authors: Johanna Catharina
authors: JC
authors: Duvigneau
authors: Wolf-Dieter
authors: WD
authors: Rausch
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D001921:Brain / Q000473:pathology*; D002478:Cells, Cultured; D004396:Coloring Agents; D003300:Copper / Q000378:metabolism; D004996:Ethidium / Q000031:analogs & derivatives; D018691:Excitatory Amino Acid Antagonists; D005260:Female; D005456:Fluorescent Dyes; D018698:Glutamic Acid / Q000187:drug effects* / Q000633:toxicity*; D007501:Iron / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D009924:Organ Culture Techniques; D010851:Picrates; D011247:Pregnancy; D011419:Propidium; D000077185:Resveratrol; D013054:Spectrophotometry, Atomic; D013267:Stilbenes / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004396:Coloring Agents; D018691:Excitatory Amino Acid Antagonists; D005456:Fluorescent Dyes; D010851:Picrates; D013267:Stilbenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C067883:dihydroethidium; D011419:Propidium; D018698:Glutamic Acid; D003300:Copper; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D014446:Tyrosine 3-Monooxygenase; D004996:Ethidium; C022616:thiazolyl blue; D000077185:Resveratrol