title: Large outbreak of cholera caused by El Tor variant Vibrio cholerae O1 in the eastern coast of Odisha, India during 2009.
abstract: A large outbreak of cholera reported during April-July 2009 in the Kendrapada district of Odisha, India was investigated. Forty-one rectal swabs and 41 water samples, collected from diarrhoeal patients and from different villages were bacteriologically analysed for the isolation of bacterial enteriopathogens, antibiogram profile and detection of various toxic genes. The bacteriological analysis of rectal swabs and environmental water samples revealed the presence of V. cholerae O1 Ogawa biotype El Tor. The V. cholerae strains were resistant to ciprofloxacin, co-trimoxazole, chloramphenicol, streptomycin, ampicillin, furazolidone and nalidixic acid. The multiplex polymerase chain reaction (PCR) assay on V. cholerae strains revealed the presence of ctxA and tcpA genes. The mismatch amplification of mutation assay (MAMA) PCR on clinical and environmental isolates of V. cholerae revealed that the strains were El Tor biotype, which harboured the ctxB gene of the classical strain. The random amplified polymorphic DNA PCR analysis and pulsed-field gel electrophoresis results indicated that the V. cholerae isolates belonged to the same clone. This investigation gives a warning that the El Tor variant of V. cholerae has spread to the coastal district causing a large outbreak that requires close monitoring and surveillance on diarrhoeal outbreaks in Odisha.
authors: Microbiology Division, Regional Medical Research Centre, ICMR, Odisha, India.
authors: B B
authors: BB
authors: Pal
authors: H K
authors: HK
authors: Khuntia
authors: S K
authors: SK
authors: Samal
authors: A S
authors: AS
authors: Kerketta
authors: S K
authors: SK
authors: Kar
authors: M
authors: M
authors: Karmakar
authors: B
authors: B
authors: Pattnaik
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002771:Cholera / Q000453:epidemiology*; D002772:Cholera Toxin / Q000235:genetics; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012007:Rectum / Q000382:microbiology; D041241:Vibrio cholerae O1 / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D002772:Cholera Toxin