title: Drosophila CIAPIN1 homologue is required for follicle cell proliferation and survival.
abstract: BACKGROUND
  The conserved cytokine-induced apoptosis inhibitor-1 (CIAPIN1) gene has been implicated in several processes, such as apoptosis, cell division, angiogenesis and Fe/S protein biogenesis. In this study, we identified the Drosophila CIAPIN1 homologue (D-CIAPIN1) and studied its role in ovarian development.
  
  
  RESULTS
  We found that D-CIAPIN1 is conserved as it can complement the nonviability of the yeast CIAPIN1-deletion strain. Several D-CIAPIN1 alleles were identified, including one allele in which that codon encoding the highly conserved twin cysteine CX2 C motif is mutated, demonstrating for the first time the importance of this motif to protein function. We demonstrated D-CIAPIN1 is an essential gene required for ovarian development. We found that D-CIAPIN1 female mutants are sterile, containing rudimentary ovaries. We noted a decrease in follicle cell numbers in D-CIAPIN1 mutant egg chambers. We further demonstrated that the decrease in follicle cell numbers in D-CIAPIN1 mutants is due to a reduced mitotic index and enhanced cell death.
  
  
  CONCLUSIONS
  Our study reveals that D-CIAPIN1 is essential for egg chamber development and is required for follicle cell proliferation and survival.
authors: Department of Life Sciences, Ben-Gurion University, Beer-Sheva, Israel.
authors: Orly
authors: O
authors: Marzuk
authors: Gabriella
authors: G
authors: Peretz
authors: Anna
authors: A
authors: Bakhrat
authors: Uri
authors: U
authors: Abdu
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D016923:Cell Death; D049109:Cell Proliferation; D002470:Cell Survival; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000502:physiology*; D008938:Mitosis; D009154:Mutation; D006080:Ovarian Follicle / Q000196:embryology*; D019076:Transgenes
chemical_list: C585347:CIAPIN1 protein, Drosophila; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins