title: A novel model for simultaneous study of neointestinal regeneration and intestinal adaptation.
abstract: The use of autologous grafts, fabricated from tissue-engineered neointestine, to enhance insufficient compensation of intestinal adaptation for severe short bowel syndrome is a compelling idea. Unfortunately, current approaches and knowledge for neointestinal regeneration, unlike intestinal adaptation, are still unsatisfactory. Thus, we have designed a novel model of intestinal adaptation with simultaneous neointestinal regeneration and evaluated its feasibility for future basic research and clinical application. Fifty male Sprague-Dawley rats weighing 250-350 g underwent this procedure and sacrificed at 4, 8, and 12 weeks postoperatively. Spatiotemporal analyses were carried out by gross, histology, and DNA/protein quantification. Three rats died of operative complications. In early experiments, the use of hard silicone stent as tissue scaffold in 11 rats was unsatisfactory for neointestinal regeneration. In later experiments, when a soft silastic tube was used, the success rate increased up to 90.9%. Further analyses revealed that no neointestine developed without donor intestine; regenerated lengths of mucosa and muscle were positively related to time postsurgery but independent of donor length with 0.5 or 1 cm. Other parameters of neointestinal regeneration or intestinal adaptation showed no relationship to both time postsurgery and donor length. In conclusion, this is a potentially important model for investigators searching for solutions to short bowel syndrome.
authors: Division of General Surgery, Chang Gung Memorial Hospital, Keelung, Taiwan. jowsc@adm.cgmh.org.tw
authors: Shyh-Chuan
authors: SC
authors: Jwo
authors: Shye-Jye
authors: SJ
authors: Tang
authors: Jim-Ray
authors: JR
authors: Chen
authors: Kun-Chun
authors: KC
authors: Chiang
authors: Ting-Shou
authors: TS
authors: Huang
authors: Huang-Yang
authors: HY
authors: Chen
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000473:pathology / Q000502:physiology* / Q000637:transplantation; D007422:Intestines / Q000473:pathology / Q000502:physiology* / Q000637:transplantation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D012778:Short Bowel Syndrome / Q000235:genetics / Q000473:pathology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials