title: A rapid method to isolate soluble royal jelly proteins.
abstract: Soluble royal jelly (RJ) proteins (SRJPs) include the major RJ protein (MRJP) family, which contribute to the physiological actions of RJ. Although SRJPs are prepared using conventional methods involving dialysis and centrifugation, dialysis is a time-consuming process. We have therefore developed a simple method to isolate SRJPs from RJ. This new method produces 20-fold higher levels of SRJPs than that of the conventional procedure; hence, the levels obtained by the new and existing methods were compared. A 1-h ultracentrifugation separated SRJPs in the supernatant into upper, middle and lower layers. Each layer was analyzed by size-exclusion HPLC, SDS-PAGE and 2-DE. The upper and middle layers contained MRJP2 (52 kDa) and MRJP3 (60-70 kDa), while the lower layer contained MRJP1 (290 kDa). In nature, MRJP1 is a monomer and/or oligomer. When the lower layer was analyzed by Superose 12 HPLC, MRJP1 was predominantly an oligomer. Our MRJP isolation method reduces the procedure time by using ultracentrifugation without dialysis to obtain SRJPs and produces layers containing MRJP1 oligomers, MRJP2 and MRJP3.
authors: Division of Gastroenterologic and General Surgery, Department of Surgery, Asahikawa Medical University, Asahikawa, Japan.
authors: Reo
authors: R
authors: Nozaki
authors: Shogo
authors: S
authors: Tamura
authors: Aimi
authors: A
authors: Ito
authors: Takanori
authors: T
authors: Moriyama
authors: Kikuji
authors: K
authors: Yamaguchi
authors: Toru
authors: T
authors: Kono
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D014461:Ultracentrifugation / Q000379:methods*; D000099219:Royal Jelly
chemical_list: D005227:Fatty Acids; D019476:Insect Proteins; D000099219:Royal Jelly