title: The relationship of metalloproteinase gene polymorphisms and lung cancer.
abstract: BACKGROUND
  We analyzed the relationship between matrix metalloproteinase (MMP)-2, -7, and -13 gene expression and polymorphisms and disease susceptibility and prognosis in patients who had undergone surgery for non-small-cell lung cancers.
  
  
  METHODS
  The study group consisted of 132 patients who had undergone radical surgery for non-small-cell lung cancers. The control group consisted of 80 healthy volunteers. We isolated deoxyribonuclease samples for use in analyzing gene polymorphisms from pathology blocks for the patient group and from blood samples for the control group. We identified MMP gene polymorphisms with polymerase chain reaction and restriction fragment length polymorphisms. Results were compared with those of the control group to evaluate disease susceptibility, correlation with other clinical parameters, and with survival and prognosis by using appropriate statistical methods.
  
  
  RESULTS
  When we compared polymorphisms pertaining to MMP genes in healthy controls and lung tumor DNA, we observed a decrease in the MMP-2 (-735) polymorphism GG genotype and increases in the MMP-13 (A77G) polymorphism AG and GG genotypes (P = 0.008, P = 0.047, and P = 0.047, respectively). For the MMP-7 (-181) polymorphism, the genotype did not differ significantly for disease susceptibility. Median overall survival time was 25.5 mo in the MMP-13 AA/AG genotypes and 9.3 mo in the GG genotype.
  
  
  CONCLUSIONS
  Decreases in the MMP-2 (-735) polymorphism GG genotype and increases in the MMP-13 (A77G) polymorphism AG and GG genotypes increase the risk for lung cancer. Furthermore, the presence of the MMP-13 (A77G) polymorphism GG genotype is an unfavorable prognostic factor.
authors: Department of Thoracic Surgery, Gaziantep University, Gaziantep, Turkey. sanli@gantep.edu.tr
authors: Maruf
authors: M
authors: Sanli
authors: Erkan
authors: E
authors: Akar
authors: Sacide
authors: S
authors: Pehlivan
authors: Kemal
authors: K
authors: Bakır
authors: Bulent
authors: B
authors: Tuncozgur
authors: Ahmet Feridun
authors: AF
authors: Isik
authors: Mustafa
authors: M
authors: Pehlivan
authors: Levent
authors: L
authors: Elbeyli
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000235:genetics*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020783:Matrix Metalloproteinase 7 / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D012189:Retrospective Studies; D012307:Risk Factors; D015996:Survival Rate; D046888:Tissue Array Analysis
chemical_list: D053509:Matrix Metalloproteinase 13; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2
keywords: Expression; Lung cancer; Matrix metalloproteinases; Polymorphism