title: The suppressive role of p38 MAPK in cellular vacuole formation.
abstract: Vacuolization of the cytoplasm is one of the dramatic and frequently observed phenomena in various cell types. Cellular vacuoles occur spontaneously or via a wide range of inductive stimuli, but the molecular mechanism involved in this process remains largely unknown. In this study, we investigated the role of the p38 and JNK pathways in the formation of cytoplasmic vacuoles. We found that p38 and JNK agonist anisomycin abolishes spontaneous cytoplasmic vacuolization of HepG2 cells through p38 activation, but not through JNK activation. Importantly, blocking the activity of p38 or suppression the expression of p38 elicits cytoplasmic vacuoles formation in various cancer cells. Furthermore, cytoplasmic vacuoles induced by p38 blocking are derived from the perinuclear region. These observations provide direct evidence for a role of p38 signaling in regulating the formation of cytoplasmic vacuoles.
authors: Department of Public Health of Luzhou Medical College, Luzhou, Sichuan, China.
authors: Run
authors: R
authors: Chen
authors: Chun-Yan
authors: CY
authors: Duan
authors: Shao-Kun
authors: SK
authors: Chen
authors: Chun-Yan
authors: CY
authors: Zhang
authors: Tao
authors: T
authors: He
authors: Hong
authors: H
authors: Li
authors: You-Ping
authors: YP
authors: Liu
authors: Rong-Yang
authors: RY
authors: Dai
mesh_terms: D000841:Anisomycin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004789:Enzyme Activation / Q000502:physiology; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D000841:Anisomycin; D048051:p38 Mitogen-Activated Protein Kinases