title: Daidzein affects steroidogenesis and oestrogen receptor expression in medium ovarian follicles of pigs.
abstract: Daidzein, a phytoestrogen present in soybean products used in swine feed, has been demonstrated to affect both reproductive and endocrine functions. The aims of this study were to examine the in vitro effects of daidzein on (1) progesterone (P4) and oestradiol (E2) secretion by porcine luteinised granulosa cells harvested from medium follicles, and (2) the mRNA and protein expression of oestrogen receptors α and β (ERα and ERβ) in these cells. The influence of E2 on P4 secretion and ERα and ERβ expression in the granulosa cells of pigs was also investigated. It was found that daidzein inhibited progesterone secretion by luteinised granulosa cells isolated from medium follicles. In contrast, E2 did not affect progesterone production by these cells. Moreover, daidzein did not alter the granulosal secretion of E2. Both daidzein and E2 decreased mRNA expression of ERα in the cells examined. The expression of ERβ mRNA was not affected by daidzein but was inhibited by E2. ERα protein was not detected while ERβ protein was found in the nuclei of the cells. Daidzein and E2 upregulated the expression of ERβ protein in the cells. In summary, the phytoestrogen daidzein directly affected the porcine ovary by inhibiting progesterone production and increasing ERβ protein expression. Daidzein-induced changes in follicular steroidogenesis and granulosal sensitivity to oestrogens may disturb reproductive processes in pigs.
authors: University of Warmia and Mazury Department of Animal Physiology Olsztyn Poland University of Warmia and Mazury Research and Educational Centre, Laboratory of Molecular Diagnostics Olsztyn Poland.
authors: Anna
authors: A
authors: Nynca
authors: Dominika
authors: D
authors: Słonina
authors: Olga
authors: O
authors: Jablońska
authors: Barbara
authors: B
authors: Kamińska
authors: Renata E
authors: RE
authors: Ciereszko
mesh_terms: D000818:Animals; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D005260:Female; D006080:Ovarian Follicle; D010053:Ovary; D011374:Progesterone; D011960:Receptors, Estrogen; D013552:Swine
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D011960:Receptors, Estrogen; D011374:Progesterone