title: The TIE1 transcriptional repressor links TCP transcription factors with TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in Arabidopsis.
abstract: Leaf size and shape are mainly determined by coordinated cell division and differentiation in lamina. The CINCINNATA (CIN)-like TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors are key regulators of leaf development. However, the mechanisms that control TCP activities during leaf development are largely unknown. We identified the TCP Interactor containing EAR motif protein1 (TIE1), a novel transcriptional repressor, as a major modulator of TCP activities during leaf development. Overexpression of TIE1 leads to hyponastic and serrated leaves, whereas disruption of TIE1 causes epinastic leaves. TIE1 is expressed in young leaves and encodes a transcriptional repressor containing a C-terminal EAR motif, which mediates interactions with the TOPLESS (TPL)/TOPLESS-RELATED (TPR) corepressors. In addition, TIE1 physically interacts with CIN-like TCPs. We propose that TIE1 regulates leaf size and morphology by inhibiting the activities of TCPs through recruiting the TPL/TPR corepressors to form a tertiary complex at early stages of leaf development.
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, PR China.
authors: Qing
authors: Q
authors: Tao
authors: Dongshu
authors: D
authors: Guo
authors: Baoye
authors: B
authors: Wei
authors: Fan
authors: F
authors: Zhang
authors: Changxu
authors: C
authors: Pang
authors: Hao
authors: H
authors: Jiang
authors: Jinzhe
authors: J
authors: Zhang
authors: Tong
authors: T
authors: Wei
authors: Hongya
authors: H
authors: Gu
authors: Li-Jia
authors: LJ
authors: Qu
authors: Genji
authors: G
authors: Qin
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D056970:Co-Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D028861:Nuclear Pore Complex Proteins; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D025941:Protein Interaction Mapping / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D028861:Nuclear Pore Complex Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C585559:TCP10 protein, Arabidopsis; C585558:TIE1 protein, Arabidopsis; C524167:TPR protein, Arabidopsis; C101995:cycloidea protein, Antirrhinum; C511291:At1g15750 protein, Arabidopsis