title: Frequent expression of follicular dendritic cell markers in Hodgkin lymphoma and anaplastic large cell lymphoma.
abstract: OBJECTIVE
  Although the tumour cells of Hodgkin lymphoma (HL) are derived from mature B-cells, the lineage infidelity of Hodgkin/Reed-Sternberg cells (HRSs) often causes diagnostic problems. Recently introduced HRS markers are also positive for follicular dendritic cells (FDCs). We investigated the expression of several FDC markers in HL and anaplastic large cell lymphoma (ALCL) and evaluated their diagnostic efficacy.
  
  
  METHODS
  Eighty-five cases of HL and 52 cases of ALCL were included in this study. Immunohistochemistry was performed for glioma-associated homologue (GLI) 3, class III β-tubulin (TUBB3), fascin, clusterin, γ-synuclein, podoplanin, syntenin, CD21, CD35 and EGFR.
  
  
  RESULTS
  HRSs were diffusely positive for GLI3, fascin and TUBB3; the mean positivity rates per case were 94% for GLI3, 82% for fascin, 69% for TUBB3, 17% for clusterin, 17% for γ-synuclein and 14% for syntenin. Podoplanin, CD21, CD35 and EGFR were almost negative. However, the frequency of marker expression was not associated with the histologic subtype or the presence of Epstein-Barr virus (EBV). ALCL showed a similar pattern to HL, but the overall frequency of positivity was lower than that observed in HL. The mean positivity rates were 56% for GLI3, 62% for fascin, 58% for TUBB3 and 21% for clusterin. The other markers were nearly negative. Anaplastic large cell lymphoma kinase positivity did not affect the expression rates.
  
  
  CONCLUSIONS
  This study confirmed the frequent expression of FDC markers in HL and ALCL. Especially, GLI3, fascin and TUBB3 are the most sensitive markers. Further studies are required to evaluate the association between FDCs, HRSs and ALCL cells.
authors: Department of Pathology, National Cancer Center, Goyang, Korea.
authors: Soo Hee
authors: SH
authors: Kim
authors: Ji-young
authors: JY
authors: Choe
authors: Yoonkyong
authors: Y
authors: Jeon
authors: Jooryung
authors: J
authors: Huh
authors: Hye Ra
authors: HR
authors: Jung
authors: Yoo-Duk
authors: YD
authors: Choi
authors: Hyun-Jung
authors: HJ
authors: Kim
authors: Hee Jeong
authors: HJ
authors: Cha
authors: Weon Seo
authors: WS
authors: Park
authors: Ji Eun
authors: JE
authors: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000077548:Anaplastic Lymphoma Kinase; D014408:Biomarkers, Tumor / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002648:Child; D020566:Dendritic Cells, Follicular / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D008198:Lymph Nodes / Q000473:pathology; D017728:Lymphoma, Large-Cell, Anaplastic / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D046888:Tissue Array Analysis; D014404:Tubulin / Q000378:metabolism; D055815:Young Adult; D000074290:Zinc Finger Protein Gli3
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; C502658:FSCN1 protein, human; C065683:GLI3 protein, human; D051741:Kruppel-Like Transcription Factors; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C498585:TUBB3 protein, human; D014404:Tubulin; D000074290:Zinc Finger Protein Gli3; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases
keywords: Hematopathology; Immunohistochemistry; Lymphoma