title: A cis-acting element present within the Gag open reading frame negatively impacts on the activity of the HIV-1 IRES.
abstract: Translation initiation from the human immunodeficiency virus type-1 (HIV-1) mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS)-1, a cis-acting regulatory element present within the gag open reading frame (ORF). In this study we evaluated the impact of the INS-1 on HIV-1 IRES-mediated translation initiation. Using heterologous bicistronic mRNAs, we show that the INS-1 negatively impact on HIV-1 IRES-driven translation in in vitro and in cell-based experiments. Additionally, our results show that the inhibitory effect of the INS-1 is not general to all IRESes since it does not hinder translation driven by the HCV IRES. The inhibition by the INS-1 was partially rescued in cells by the overexpression of the viral Rev protein or hnRNPA1.
authors: Laboratorio de Virología Molecular, Instituto Milenio de Inmunología e Inmunoterapia, Centro de Investigaciones Médicas, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors: Fernando
authors: F
authors: Valiente-Echeverría
authors: Maricarmen
authors: M
authors: Vallejos
authors: Anne
authors: A
authors: Monette
authors: Karla
authors: K
authors: Pino
authors: Alejandro
authors: A
authors: Letelier
authors: J Pablo
authors: JP
authors: Huidobro-Toro
authors: Andrew J
authors: AJ
authors: Mouland
authors: Marcelo
authors: M
authors: López-Lastra
mesh_terms: D015750:Genes, gag / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D016366:Open Reading Frames / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054321:rev Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D054321:rev Gene Products, Human Immunodeficiency Virus