title: Noncovalent tagging proteins with paramagnetic lanthanide complexes for protein study.
abstract: The site-specific labeling of proteins with paramagnetic lanthanides offers unique opportunities for NMR spectroscopic analysis in structural biology. Herein, we report an interesting way of obtaining paramagnetic structural restraints by employing noncovalent interaction between a lanthanide metal complex, [Ln(L)3](n-) (L=derivative of dipicolinic acid, DPA), and a protein. These complexes formed by lanthanides and DPA derivatives, which have different substitution patterns on the DPA derivatives, produce diverse thermodynamic and paramagnetic properties when interacting with proteins. The binding affinity of [Ln(L)3](n-) with proteins, as well as the determined paramagnetic tensor, are tunable by changing the substituents on the ligands. These noncovalent interactions between [Ln(L)3](n-) and proteins offer great opportunities in the tagging of proteins with paramagnetic lanthanides. We expect that this method will be useful for obtaining multiple angles and distance restraints of proteins in structural biology.
authors: State Key Laboratory of Elemento-Organic Chemistry and College of Chemistry, Nankai University, Tianjin, PR China.
authors: Zhen
authors: Z
authors: Wei
authors: Yin
authors: Y
authors: Yang
authors: Qing-Feng
authors: QF
authors: Li
authors: Feng
authors: F
authors: Huang
authors: Hui-Hui
authors: HH
authors: Zuo
authors: Xun-Cheng
authors: XC
authors: Su
mesh_terms: D056831:Coordination Complexes; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008024:Ligands; D008956:Models, Chemical; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010848:Picolinic Acids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D056831:Coordination Complexes; D028581:Lanthanoid Series Elements; D008024:Ligands; D010848:Picolinic Acids; D011506:Proteins; C004860:dipicolinic acid