title: Dodecaploid H1 embryonic stem cells abolished pluripotency in L15F10 medium both with and without leukemia inhibitory factor.
abstract: Two lines of dodecaploid H1 embryonic stem cells, 12H1 and 12H1(-) cells (mouse-originated cells), were established through polyploidization of two hexaploid H1 cells, 6H1 and 6H1(-) cells, which were cultured in L15F10 (7:3) medium with and without leukemia inhibitory factor (LIF), respectively. The G1, S, and G2/M phase fractions of 12H1 and 12H1(-) cells were almost the same as those of 6H1 and 6H1(-) cells, respectively, but the doubling time of cell proliferation was prolonged, suggesting that cell death occurred in 12H1 and 12H1(-)cells. The cell volumes of 12H1 and 12H1(-) cells were about double those of 6H1 and 6H1(-) cells, respectively. 12H1 and 12H1(-) cells showed near-negative activity of alkaline phosphatase and no ability to form teratocarcinomas in mouse abdomen, suggesting that 12H1 and 12H1(-) cells lost pluripotency. The DNA contents of 12H1 and 12H1(-) cells decayed in long-term culturing, suggesting that 12H1 and 12H1(-) cells were DNA-unstable. Possible explanations for the lost pluripotency and for the DNA decay in 12H1 and 12H1(-) cells are presented.
authors: Division of Cell Medicine, Research Institute of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa, 920-0293, Japan. fujikawa@kanazawa-med.ac.jp
authors: Kohzaburo
authors: K
authors: Fujikawa-Yamamoto
authors: Minoru
authors: M
authors: Miyagoshi
authors: Hiroko
authors: H
authors: Yamagishi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D063646:Carcinogenesis; D002453:Cell Cycle; D016923:Cell Death; D049109:Cell Proliferation; D048429:Cell Size; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004261:DNA Replication; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009930:Organic Chemicals; D011123:Polyploidy
chemical_list: C095240:L15 culture medium; D053605:Leukemia Inhibitory Factor; D009930:Organic Chemicals; D004247:DNA; D000469:Alkaline Phosphatase