title: Factors affecting mesenchymal stromal cells yield from bone marrow aspiration.
abstract: OBJECTIVE
  This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC).
  
  
  METHODS
  WE DETERMINED THE YIELD OF MSC FROM BONE MARROW UNDER DIFFERENT CLINICAL CONDITIONS BY COMPARING THE MSC COLONY NUMBERS FROM: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles.
  
  
  RESULTS
  During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.
  
  
  CONCLUSIONS
  The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.
authors: China Oxford Centre for International Health Research, Cardiovascular Institute & Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China ; Department of Paediatrics and Adolescent Medicine, the University of Hong Kong, HKSAR, China.
authors: Jing
authors: J
authors: Li
authors: Wilfred Hing-Sang
authors: WH
authors: Wong
authors: Shing
authors: S
authors: Chan
authors: James Chor-San
authors: JC
authors: Chim
authors: Kenneth Man-Chee
authors: KM
authors: Cheung
authors: Tsz-Leung
authors: TL
authors: Lee
authors: Wing-Yan
authors: WY
authors: Au
authors: Shau-Yin
authors: SY
authors: Ha
authors: Albert Kwok-Wei
authors: AK
authors: Lie
authors: Yu-Lung
authors: YL
authors: Lau
authors: Raymond Hin-Suen
authors: RH
authors: Liang
authors: Godfrey Chi-Fung
authors: GC
authors: Chan
keywords: Bone marrow aspiration; CFU-F; Leukemia; Mesenchymal stromal cell