title: Y-box binding protein-1 regulates cell proliferation and is associated with clinical outcomes of osteosarcoma.
abstract: BACKGROUND
  Prognosis of osteosarcoma (OS) with distant metastasis and local recurrence is still poor. Y-box binding protein-1 (YB-1) is a multifunctional protein that can act as a regulator of transcription and translation and its high expression of YB-1 protein was observed in OS, however, the role of YB-1 in OS remains unclear.
  
  
  METHODS
  Y-box binding protein-1 expression in OS cells was inhibited by specific small interfering RNAs to YB-1 (si-YB-1). The effects of si-YB-1 in cell proliferation and cell cycle transition in OS cells were analysed in vitro and in vivo. The association of nuclear expression of YB-1 and clinical prognosis was also investigated by immunohistochemistry.
  
  
  RESULTS
  Proliferation of OS cell was suppressed by si-YB-1 in vivo and in vitro. The expression of cyclin D1 and cyclin A were also decreased by si-YB-1. In addition, si-YB-1 induced G1/S arrest with decreased cyclin D1 and cyclin A in OS cell lines. Direct binding of YB-1 in OS cell lines was also observed. Finally, the nuclear expression of YB-1 was significantly related to the poorer overall survival in OS patients.
  
  
  CONCLUSIONS
  Y-box binding protein-1 would regulate cell cycle progression at G1/S and tumour growth in human OS cells in vitro and in vivo. Nuclear expression of YB-1 was closely associated with the prognosis of OS, thus, YB-1 simultaneously could be a potent molecular target and prognostic biomarker for OS.
authors: Department of Orthopaedic Surgery, Kyushu University, Fukuoka, Japan.
authors: Y
authors: Y
authors: Fujiwara-Okada
authors: Y
authors: Y
authors: Matsumoto
authors: J
authors: J
authors: Fukushi
authors: N
authors: N
authors: Setsu
authors: S
authors: S
authors: Matsuura
authors: S
authors: S
authors: Kamura
authors: T
authors: T
authors: Fujiwara
authors: K
authors: K
authors: Iida
authors: M
authors: M
authors: Hatano
authors: A
authors: A
authors: Nabeshima
authors: H
authors: H
authors: Yamada
authors: M
authors: M
authors: Ono
authors: Y
authors: Y
authors: Oda
authors: Y
authors: Y
authors: Iwamoto
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000401:mortality; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002648:Child; D019925:Cyclin A / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D012516:Osteosarcoma / Q000378:metabolism* / Q000401:mortality; D011379:Prognosis; D034741:RNA, Small Interfering / Q000494:pharmacology; D051840:Y-Box-Binding Protein 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D019925:Cyclin A; D034741:RNA, Small Interfering; D051840:Y-Box-Binding Protein 1; D019938:Cyclin D1