title: Serum microRNAs levels in primary focal segmental glomerulosclerosis.
abstract: BACKGROUND
  MicroRNAs (miRNAs, miRs) are involved in most physiological, developmental, and pathological processes. miR-192 and miR-205 are expressed preferentially in the renal cortex and closely relevant to the renal cell biology. In the present study, we aim to measure the serum levels of miR-192 and miR-205 and their correlation with clinicopathological data in patients with primary focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD).
  
  
  METHODS
  Fifty-six patients (35 male, 21 female) with idiopathic nephrotic syndrome (FSGS 30, MCD 26) and 20 healthy controls were enrolled in the study. We quantified the serum levels of miR-192 and miR-205 in patients with FSGS and MCD by RT-qPCR.
  
  
  RESULTS
  Patients with FSGS had higher serum levels of miR-192 and miR-205 than those with MCD (324.49 ± 42.74 fmol/l versus 90.19 ± 27.14 fmol/l, p < 0.01, 2.25 ± 0.69 fmol/l versus 0.60 ± 0.51 fmol/l, p < 0.01, respectively). The level of miR-192 was positively correlated with the proteinuria in patients with FSGS and MCD (r = 0.62, p < 0.001, r = 0.84, p < 0.001, respectively). Similarly, the level of miR-205 was positively correlated with the proteinuria in patients with FSGS (r = 0.54, p = 0.002). In addition, the serum level of miR-192 was significantly correlated with the degree of interstitial fibrosis in patients with FSGS (r = 0.342, p < 0.05).
  
  
  CONCLUSIONS
  miR-192 and miR-205 have the potential as markers to differentiate FSGS from MCD.
authors: Department of Pediatric Nephrology, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, Jiangsu Province, China.
authors: Xiaoyi
authors: X
authors: Cai
authors: Zhengkun
authors: Z
authors: Xia
authors: Chunni
authors: C
authors: Zhang
authors: Yang
authors: Y
authors: Luo
authors: Yuanfu
authors: Y
authors: Gao
authors: Zhongmin
authors: Z
authors: Fan
authors: Mengyuan
authors: M
authors: Liu
authors: Ying
authors: Y
authors: Zhang
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D002648:Child; D005260:Female; D005355:Fibrosis; D005919:Glomerular Filtration Rate; D005923:Glomerulosclerosis, Focal Segmental / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007677:Kidney Function Tests; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D009402:Nephrosis, Lipoid / Q000473:pathology; D016133:Polymerase Chain Reaction; D016896:Treatment Outcome
chemical_list: D000305:Adrenal Cortex Hormones; D035683:MicroRNAs; C546783:MIRN192 microRNA, human; C534425:MIR205, human