title: [Intestinal absorption of different combinations of active compounds from Gegenqinlian decoction by rat single pass intestinal perfusion in situ].
abstract: The aim is to study the intestinal absorption of different combinations of active compounds out of Gegenqinlian decoction. Rat single pass intestinal perfusion model with jugular vein cannulated was used. Samples were obtained continuously from the outlet perfusate and the mesenteric vein. The levels of puerarin, daidzin, liquilitin, baicalin, wogonoside, jatrorrhizine, berberine and palmatine were determined by LC-MS/MS and their permeability coefficients were calculated. The results showed that Glycyrrhiza could promote the absorption of the active ingredients in Pueraria which is the monarch herb; meanwhile, Pueraria also played a role in promoting the absorption of liquilitin. Based on the Gegenqinlian decoction and the different combinations experiments, the results concerning the absorption of baicalin and wogonoside were as follows. For baicalin, Pueraria and Glycyrrhiza could promote its absorption and the effect of Pueraria was more obvious. For wogonoside, Pueraria could also promote its absorption, while Glycyrrhiza played a opposite role. Pueraria and Glycyrrhiza both played a part in promoting the absorption of jateorhizine, berberine and palmatine, the effective compounds in Coptis.
authors: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. anruimw@126.com
authors: Rui
authors: R
authors: An
authors: Hua
authors: H
authors: Zhang
authors: Yi-Zhu
authors: YZ
authors: Zhang
authors: Ran-Chi
authors: RC
authors: Xu
authors: Xin-Hong
authors: XH
authors: Wang
mesh_terms: D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives / Q000378:metabolism; D001600:Berberine Alkaloids / Q000378:metabolism; D039323:Coptis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000493:pharmacokinetics*; D044950:Flavanones / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D006035:Glycyrrhiza / Q000737:chemistry; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D007529:Isoflavones / Q000378:metabolism; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry
chemical_list: D001600:Berberine Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D007529:Isoflavones; C542794:gegenqinlian; C055785:jatrorrhizine; D001599:Berberine; C038044:baicalin; C013908:daidzin; C473995:wogonoside; C005413:palmatine; C512196:liquiritin; C033607:puerarin