title: Induction of heat shock gene expression in RAT1 primary fibroblast cells by ELF electric fields.
abstract: Recent studies have demonstrated that the Ku70 gene fragment can be placed in the anti-sense orientation under the control of a heat-inducible heat shock protein 70 (HSP70) promoter and activated through heat shock exposure. This results in attenuation of the Ku70 protein expression, inhibiting cellular repair processes, and sensitizing the transfected cells to exposures such as the ionizing radiation exposures used clinically. However, achieving the tissue temperatures necessary to thermally induce the HSP70 response presents significant limitations to the clinical application of this strategy. Previous findings suggest an alternative approach to inducing a heat shock response, specifically through the use of extremely low frequency (ELF) electrical field stimulation. To further pursue this approach, we investigated HSP70 responses in transfected rat primary fibroblast (RAT1) cells exposed to 10 Hz electric fields at intensities of 20-500 V/m. We confirmed that low frequency electric fields can induce HSP70 heat shock expression, with peak responses obtained at 8 h following a 2 h field exposure. However, the approximate threefold increase in expression is substantially lower than that obtained using thermal stimulation, raising questions of the clinical utility of the response.
authors: Biomedical Physics and Engineering, Medical Physics Department, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
authors: Paul
authors: P
authors: Frisch
authors: Gloria C
authors: GC
authors: Li
authors: Kenneth
authors: K
authors: McLeod
authors: Craig B
authors: CB
authors: Laramee
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000235:genetics; D018929:Cell Culture Techniques; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D004574:Electromagnetic Fields; D005347:Fibroblasts / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000528:radiation effects*; D000072200:Ku Autoantigen; D008156:Luciferases; D049408:Luminescent Agents; D011401:Promoter Regions, Genetic / Q000235:genetics; D011829:Radiation Dosage; D051381:Rats; D013997:Time Factors; D015533:Transcriptional Activation / Q000528:radiation effects; D014162:Transfection
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D049408:Luminescent Agents; D008156:Luciferases; C000606511:Xrcc6 protein, rat; D000072200:Ku Autoantigen