title: Molecular mechanisms underlying the anti-obesity potential of prunetin, an O-methylated isoflavone.
abstract: Prunetin is an O-methylated isoflavone, which is a type of flavonoid. There are a limited number of reports detailing the biological activities of prunetin. Although an anti-inflammatory effect of prunetin has been reported in vitro, to our knowledge, there have been no reports on anti-adipogenic effects of prunetin in obese animals. The aims of this study were to determine whether prunetin suppresses high-fat diet (HFD)-induced adipogenesis in the liver and visceral adipose tissues of mice, and to explore the underlying mechanisms mediating the actions of prunetin. To this end, mice were fed a HFD for 10 weeks to induce obesity, and prunetin (10 μg/kg or 20 μg/kg) was administered in the last 3 weeks. Compared to saline-treated mice, mice treated with prunetin showed significantly reduced body weight gain, visceral fat pad weights, and plasma glucose levels. We found that prunetin significantly inhibited the HFD-induced upregulation of the expression of important adipogenic genes (PPARγ, C/EBPα, SREBP, aP2, LPL adiponectin, and leptin), and suppressed HFD-mediated increase in expression of lipid metabolism-related genes (SREBP, PPARγ, LXR, and HMG-CoA) in the liver tissues. Furthermore, prunetin induced expression of adiponectin receptors 1 and 2 (adipoR1, adipoR2), as well as that of AMP-activated protein kinase (AMPK) in the liver and adipose tissue. These results suggest that prunetin mediates anti-obesity/adipogenesis effects by suppressing obesity-related transcription through a feedback mechanism that regulates the expression of adiponectin, adipoR1, adipoR2, and AMPK.
authors: Department of Pharmacology, College of Oriental Medicine, Sangji University, Wonju-si, Gangwon-do 220-702, Republic of Korea.
authors: Tae-Gue
authors: TG
authors: Ahn
authors: Gabsik
authors: G
authors: Yang
authors: Heon-Myung
authors: HM
authors: Lee
authors: Myung-Dong
authors: MD
authors: Kim
authors: Ho-Young
authors: HY
authors: Choi
authors: Kyoung-Sik
authors: KS
authors: Park
authors: Sun-Dong
authors: SD
authors: Lee
authors: Yoon-Bum
authors: YB
authors: Kook
authors: Hyo-Jin
authors: HJ
authors: An
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D052242:Adiponectin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D025461:Feedback, Physiological; D005786:Gene Expression Regulation / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D054419:Receptors, Adiponectin / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D019440:Anti-Obesity Agents; D001786:Blood Glucose; D007529:Isoflavones; D054419:Receptors, Adiponectin; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; C083295:prunetin; D055372:AMP-Activated Protein Kinases