title: Novel indium-111 labeled gastrin peptide analogues (MG-CL1-4): synthesis and quality control.
abstract: Radiolabeled neuropeptides are widely investigated to diagnose and therapy of tumors. These peptides get internalization after binding with particular receptors at the surface of cells and finally move to lysosome. Internalization into tumor cells helps in mapping the infected site. Minigastrin peptide analogues (MG-CL1-4) were synthesised and labeled with 111-In radioisotope under different sets of conditions for imaging CCk-2 receptor bearing tumors. Different parameters such as temperature (80-100°C), pH (4-12), incubation time (5-30 minutes) and dilution effect were investigated to get the maximum labeling yield and stability. The results indicated that MG-CL1-4 is successfully labeled with indium-111 at pH 4.5 with heating at 98°C for 15 minute. At these conditions i.e. heating, pH and incubation minimum oxidized and maximum labeling yield, more than 94 %, was obtained. The labeling stability was studied by incubating the radiolabeled complex for predefined time points in PBSA and blood serum. Results show that more than 90% radiolabeled MG-CL1-4 remained intact.
authors: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors: Syed Ali-Raza
authors: SA
authors: Naqvi
authors: Zulfiqar Ali
authors: ZA
authors: Khan
authors: Saeed Ahmad
authors: SA
authors: Nagra
authors: Muhammad
authors: M
authors: Yar
authors: Tauqir A
authors: TA
authors: Sherazi
authors: Sohail Shahzad
authors: SS
authors: Shahzad
authors: Syed Qaiser
authors: SQ
authors: Shah
authors: Nasir
authors: N
authors: Mahmood
authors: Malik Muhammad
authors: MM
authors: Ishfaq
authors: Stephen John
authors: SJ
authors: Mather
mesh_terms: D005755:Gastrins / Q000097:blood / Q000138:chemical synthesis* / Q000592:standards; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000592:standards; D010084:Oxidation-Reduction; D055550:Protein Stability; D011786:Quality Control; D013696:Temperature; D013997:Time Factors
chemical_list: D005755:Gastrins; D007205:Indium Radioisotopes; C008530:minigastrin