title: Immunomodulatory effects of human umbilical cord Wharton's jelly-derived mesenchymal stem cells on differentiation, maturation and endocytosis of monocyte-derived dendritic cells.
abstract: The Wharton's jelly of the umbilical cord is believed to be a source of mesenchymal stem cells (MSCs) which can be therapeutically applied in degenerative diseases.In this study, we investigated the immunomodulatory effect of umbilical cord derived-mesenchymal stem cells (UC-MSCs) and bone marrow-derived-mesenchymal stem cells (BM-MSCs) on differentiation, maturation, and endocytosis of monocyte-derived dendritic cells in a transwell culture system under laboratory conditions. Monocytes were differentiated into immature dendritic cells (iDCs) in the presence of GM-CSF and IL-4 for 6 days and then differentiated into mature dendritic cells (mDCs) in the presence of TNF-α for 2 days. In every stage of differentiation, immature and mature dendritic cells were separately co-cultured with UC-MSCs and BM-MSCs. The findings showed that UC-MSCs and BM-MSCs inhibited strongly differentiation and maturation of dendritic cells at higher dilution ratios (1:1). The BM-MSCs and UC-MSCs showed more inhibitory effect on CD1a, CD83, CD86 expression, and dendritic cell endocytic activity, respectively. On the other hand, these cells severely up-regulated CD14 marker expression. We concluded that UC-MSCs and BM-MSCs could inhibit differentiation, maturation and endocytosis in monocyte-derived DCs through the secreted factors and free of any cell-cell contacts under laboratory conditions. As DCs are believed to be the main antigen presenting cells for naïve T cells in triggering immune responses, it would be logical that their inhibitory effect on differentiation, maturation and function can decrease or modulate immune and inflammatory responses.
authors: Molecular Immunology Research Center and Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran. nikbinbe@sina.tums.ac.ir.
authors: Mohsen
authors: M
authors: Saeidi
authors: Ahmad
authors: A
authors: Masoud
authors: Yadollah
authors: Y
authors: Shakiba
authors: Jamshid
authors: J
authors: Hadjati
authors: Mandana
authors: M
authors: Mohyeddin Bonab
authors: Mohammad Hossein
authors: MH
authors: Nicknam
authors: Mostafa
authors: M
authors: Latifpour
authors: Behrooz
authors: B
authors: Nikbin
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D015194:Diffusion Chambers, Culture; D004705:Endocytosis; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D056747:Immunomodulation; D015847:Interleukin-4 / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D010011:Osteocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D061251:Primary Cell Culture; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014470:Umbilical Cord / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D059631:Wharton Jelly / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
chemical_list: D015703:Antigens, CD; C508598:IL4 protein, human; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor