title: Upper airway inflammation exacerbates bronchial hyperreactivity in mouse models of rhinosinusitis and allergic asthma.
abstract: BACKGROUND
  Recent studies have suggested that upper airway inflammation has a strong impact on lower airway diseases. The purpose of this study was to assess whether nasal inflammation could exacerbate allergic asthma in a mouse model.
  
  
  METHODS
  Mice were assigned to 4 groups: control (Cont), either rhinosinusitis (R) or allergic asthma (A) alone, and both rhinosinusitis and allergic asthma (R&A). Mice underwent induction of nasal inflammation (R and R&A) or sham surgery (Cont and A) on day 1. Mice in the A and R&A groups were sensitized to ovalbumin on days 1, 7, and 14, followed by aerosol challenge on days 18 to 20, whereas in the Cont and R groups only saline was administered. All mice were assessed for airway hyperresponsiveness (AHR) and were euthanized on day 21. The sera, bronchoalveolar lavage fluids (BALFs), and nasal and lung tissues were collected for further analyses.
  
  
  RESULTS
  Histology findings confirmed upper and lower airway inflammation in experimental mice. Significantly increased AHR and total serum immunoglobulin E (IgE) were observed in the R&A group when compared with those of the Cont, R, and A groups. Responses to IgG2a induction were also found in sera and BALFs from mice with rhinosinusitis (R and R&A). Higher levels of interleukin 4 (IL-4) and IL-13, and increased eosinophilic inflammation were detected in BALFs and lung tissues from the experimental groups when compared with those from the Cont group.
  
  
  CONCLUSIONS
  Our results confirm that upper airway inflammation could exacerbate allergic asthma, and provide support to the concept of "one airway, one disease.
authors: Department of Otolaryngology, Taichung Veterans General Hospital, Taichung, Taiwan.
authors: Kai-Li
authors: KL
authors: Liang
authors: Rong-San
authors: RS
authors: Jiang
authors: Ren-Ching
authors: RC
authors: Wang
authors: Malcolm
authors: M
authors: Koo
authors: Shyh-Chang
authors: SC
authors: Chen
authors: Wan-Chun
authors: WC
authors: Huang
authors: Yueh-Chiao
authors: YC
authors: Yeh
mesh_terms: D000485:Allergens / Q000008:administration & dosage; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D018793:Interleukin-13 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009296:Nasal Cavity / Q000473:pathology; D010047:Ovalbumin / Q000008:administration & dosage; D012220:Rhinitis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D012852:Sinusitis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000485:Allergens; D007074:Immunoglobulin G; D018793:Interleukin-13; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: allergen; allergic rhinitis; asthma; bronchial hyperreactivity; mouse; ovalbumin; rhinosinusitis