title: Overestimated accuracy of circular dichroism in determining protein secondary structure.
abstract: Circular dichroism (CD) is a spectroscopic technique widely used for estimating protein secondary structures in aqueous solution, but its accuracy has been doubted in recent work. In the present paper, the contents of nine globular proteins with known secondary structures were determined by CD spectroscopy and Fourier transform infrared spectroscopy (FTIR) in aqueous solution. A large deviation was found between the CD spectra and X-ray data, even when the experimental conditions were optimized. The content determined by FTIR was in good agreement with the X-ray crystallography data. Therefore, CD spectra are not recommended for directly calculating the content of a protein's secondary structure.
authors: Department of Chemistry, Fudan University, Shanghai, 200433, China.
authors: Kailei
authors: K
authors: Lin
authors: Huayan
authors: H
authors: Yang
authors: Zhengya
authors: Z
authors: Gao
authors: Feng
authors: F
authors: Li
authors: Shaoning
authors: S
authors: Yu
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002942:Circular Dichroism / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry; D006736:Horses; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D011817:Rabbits; D015203:Reproducibility of Results; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013696:Temperature; D014965:X-Rays
chemical_list: D006454:Hemoglobins; D009211:Myoglobin; D011506:Proteins; D012259:Ribonuclease, Pancreatic; D009113:Muramidase