title: Optimization of hydrolysis conditions for the production of glucomanno-oligosaccharides from konjac using β-mannanase by response surface methodology.
abstract: Glucomanno-oligosaccharides (GMO), usually produced from hydrolysis of konjac tubers with a high content of glucomannan, have a positive effect on Bifidobacterium as well as a variety of other physiological activities. Response surface methodology (RSM) was employed to optimize the hydrolysis time, hydrolysis temperature, pH and enzyme to substrate ratio (E/S) to obtain a high GMO yield from konjac tubers. From the signal-factor experiments, it was concluded that the change in the direct reducing sugar (DRS) is consistent with total reducing sugar (TRS) but contrary to the degree of polymerization (DP). DRS was used as an indicator of the content of GMO in the RSM study. The optimum RSM operating conditions were: reaction time of 3.4 h, reaction temperature of 41.0°C, pH of 7.1 and E/S of 0.49. The results suggested that the enzymatic hydrolysis was enhanced by temperature, pH and incubation time. Model validation showed good agreement between experimental results and the predicted responses.
authors: Key Laboratory of Functional Dairy Science of Beijing and Ministry of Education, College of Food Science & Nutritional Engieering, China Agricultural University, Beijing, 100083, China.
authors: Junfan
authors: J
authors: Chen
authors: Desheng
authors: D
authors: Liu
authors: Bo
authors: B
authors: Shi
authors: Hai
authors: H
authors: Wang
authors: Yongqiang
authors: Y
authors: Cheng
authors: Wenjing
authors: W
authors: Zhang
mesh_terms: D031056:Amorphophallus / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D035281:Plant Tubers / Q000737:chemistry; D058105:Polymerization; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors; D044902:beta-Mannosidase / Q000737:chemistry*
chemical_list: D008351:Mannans; D009844:Oligosaccharides; C022901:(1-6)-alpha-glucomannan; D044902:beta-Mannosidase