title: Preparation of ZnFe2O4 nanoparticles in the template of silk-fibroin peptide and their neuro-cytocompability in PC12 cells.
abstract: There is an urgency to prepare non-neurotoxic nanoparticles (NPs) as MRI contrast agent. We prepared ZnFe2O4 NPs with monocrystal structure in the template of silk-fibroin (SF) peptide at room temperature. Analyses of their crystallite size and NPs diameter after the growth of 1 d and 8 d indicated that SF peptide could promote nucleation, growth and dispersion of NPs. Results of EDS, Raman and TG proved that there was 4 wt% of the chemical composition of SF in as-prepared NPs. A possible growth mechanism of ZnFe2O4 NPs in the template of SF is proposed. The saturation magnetizations of NPs with SF (SF-NPs) were about 15 and 30 emu/g for 1 and 8 d, respectively. SF-NPs obviously promoted the viability of PC12 cells at NPs concentration of 0.0625 mg/mL in 2 d and 5 d of co-culture. Analysis of neurite length indicated no significant decreases of the experimental groups at different SF-NPs concentrations (from 0.0625 to 0.5 mg/mL) after 5 d cell co-culture, because SF peptide coating could slow the release of iron/zinc ions from NPs. Furthermore, SF-NPs did not destroy the organelles, karyotheca and the neurite using the observation in the cell ultrathin sections. Based on their good magnetic property and neuro-cytocompatibility, the potential application of ZnFe2O4 NPs with SF as MRI contrast agents would be envisioned.
authors: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
authors: Juan
authors: J
authors: Liu
authors: Min
authors: M
authors: Deng
authors: Zhongbing
authors: Z
authors: Huang
authors: Guangfu
authors: G
authors: Yin
authors: Xiaoming
authors: X
authors: Liao
authors: Jianwen
authors: J
authors: Gu
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005290:Ferric Compounds / Q000737:chemistry*; D005349:Fibroins / Q000494:pharmacology*; D008903:Minerals / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016501:Neurites / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*; D016716:PC12 Cells; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D013059:Spectrum Analysis, Raman; D013997:Time Factors; D014961:X-Ray Diffraction; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D008903:Minerals; D010087:Oxides; D010455:Peptides; D017967:Zinc Compounds; D005349:Fibroins; D015032:Zinc