title: Association between EGF, TGF-β1 and TNF-α gene polymorphisms and hepatocellular carcinoma.
abstract: BACKGROUND
  Up to present, EGF 61*A/G, TGF-β1 -509*T/C and TNF-α -308*A/G gene polymorphisms have been analysed in other cancer entities than hepatocellular carcinoma (HCC). We here investigated the frequency of these gene polymorphisms among HCC patients.
  
  
  METHODS
  A total of 73 HCC patients and 117 cancer-free healthy people were recruited at the Surgical Department of Zhongshan Hospital. Genomic DNA was isolated from peripheral blood and gene polymorphisms were analyzed by PCR-RFLP.
  
  
  RESULTS
  The distribution of EGF 61*G/G homozygotes among HCC patients was more frequent than that in the control group (24.7% vs 11.1%, OR=2.618, 95%CI=1.195-5.738). In parallel, the frequency of the "G" allele in the HCC patient group was also higher than that in the control group (45.9% vs 33.3%, OR= 1.696, 95%CI=1.110-2.592). No difference could be found for the TGF-β1-509 and TNF-α -308 genotypes.
  
  
  CONCLUSIONS
  EGF 61*G/G genotype and G allele are significantly increased among patients with HCC. TGF-β1-509*T/C and TNF-α -308*A/G gene polymorphisms are not related to this cancer entity.
authors: Department of General Surgery, Binzhou Central Hospital, Binzhou Medical College, Bin Zhou, China.
authors: Hai-Zhou
authors: HZ
authors: Shi
authors: Peng
authors: P
authors: Ren
authors: Qing-Jun
authors: QJ
authors: Lu
authors: Marco
authors: M
authors: Niedrgethmnn
authors: Guo-Yang
authors: GY
authors: Wu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics; D004815:Epidermal Growth Factor / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor