title: Effects of pulsed infra-red low level-laser irradiation on mast cells number and degranulation in open skin wound healing of healthy and streptozotocin-induced diabetic rats.
abstract: BACKGROUND
  Low-level laser therapy (LLLT) has been reported to be capable of changing mast cell numbers and degranulation in experimental wounds.
  
  
  OBJECTIVE
  We conducted a study on the influence of pulsed LLLT on mast cells in wounds of non-diabetic and diabetic rats.
  
  
  METHODS
  Thirty-six rats were randomly divided into non-diabetic and diabetic groups. Type 1 diabetes milletes (DM) was induced in rats of the diabetic group by administration of streptozotocin (STZ). We inflicted two wounds in each rat. The first wound in both non-diabetic and diabetic groups was treated with an 890-nm laser, having pulse frequency of 80 Hz and energy density of 0.2 J/cm(2). Mast cell numbers and degranulation of all subgroups were assessed at 4, 7, and 15 days after the infliction of the wounds.
  
  
  CONCLUSIONS
  According to the paired t-test, the total number of laser-treated mast cells was significantly higher than that of the placebos in the non-diabetic groups on days 4 and 15. The total number of laser-treated mast cells was significantly higher than that of the placebos in the diabetic groups on days 4 and 15. The number of granulated mast cells was significantly higher than that of degranulated mast cells for all laser-treated mast cells and placebo mast cells of the non-diabetic and diabetic groups.
authors: Anatomy and Cell Biology Department, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors: Fateme Fadaie
authors: FF
authors: Fathabadie
authors: Mohammad
authors: M
authors: Bayat
authors: Abdolah
authors: A
authors: Amini
authors: Maryam
authors: M
authors: Bayat
authors: Fatemealsadat
authors: F
authors: Rezaie
mesh_terms: D000818:Animals; D002452:Cell Count; D015550:Cell Degranulation; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000503:physiopathology*; D007259:Infrared Rays; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008407:Mast Cells / Q000502:physiology / Q000528:radiation effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000528:radiation effects*; D013311:Streptozocin; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D013311:Streptozocin