title: A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules.
abstract: Based on the consideration that compound 1 with a -PO3H2 group can be hydrolyzed into compound 3 which shows low solubility in aqueous solutions and thus aggregation can occur easily, a new fluorescence turn-on assay has been constructed for alkaline phosphatase (ALP) with compound 1. ALP at concentrations as low as 18 mU mL(-1) can be assayed with compound 1. Moreover, compound 1 has been successfully applied for ALP assay in living cells. Also, compound 1 is useful for screening inhibitors of ALP.
authors: Beijing National Laboratory for Molecular Sciences, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Xinggui
authors: X
authors: Gu
authors: Guanxin
authors: G
authors: Zhang
authors: Zhuo
authors: Z
authors: Wang
authors: Wenwen
authors: W
authors: Liu
authors: Le
authors: L
authors: Xiao
authors: Deqing
authors: D
authors: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors* / Q000737:chemistry; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000032:analysis*; D005030:Ethylenes / Q000737:chemistry; D005470:Fluorometry; D006367:HeLa Cells; D006801:Humans
chemical_list: D004791:Enzyme Inhibitors; D005030:Ethylenes; C036216:ethylene; D000469:Alkaline Phosphatase