title: Microsphere-based flow cytometric immunoassay for the determination of citrinin in red yeast rice.
abstract: The determination of citrinin (CIT) by a microsphere-based flow cytometric immunoassay (MFCI) has been developed. In the method, the carboxyl-modified microspheres were conjugated with CIT-Ovalbumin (OVA) antigen. CIT competed with the CIT-OVA antigen on the surface of the microspheres for the anti-CIT McAb. Under the optimised conditions, IC(50) value was 1.0 ng/mL and the limit of detection reached 0.005 ng/mL. The cross-reactivity was less than 0.01% against each of the four mycotoxins such as aflatoxin B(1) (AFB(1)), ochratoxin A (OTA), zearalenone (ZEA), deoxynilvalenol (DON). In the work, the MFCI could accurately determine CIT in the real red yeast rice. The systematic error was low with the coefficient of variation (CV) from 5.24% to 8.16% by the MFCI. The mean recovery of CIT from artificially contaminated red yeast rice was from 89% to 94%, with CV from 7.2% to 8.7%. The experimental data showed that the precision, sensitivity and specificity of the developed MFCI method for the determination of CIT were satisfactory.
authors: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, PR China.
authors: Yongning
authors: Y
authors: Li
authors: Haiyan
authors: H
authors: Wu
authors: Liangqia
authors: L
authors: Guo
authors: Yunquan
authors: Y
authors: Zheng
authors: Yanghao
authors: Y
authors: Guo
mesh_terms: D001688:Biological Products / Q000032:analysis*; D002953:Citrinin / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*
chemical_list: D001688:Biological Products; D009183:Mycotoxins; C117343:red yeast rice; D002953:Citrinin