title: [Effect of quercetin on invasion, migration, proliferation and cell cycle of glioma U87 cells].
abstract: OBJECTIVE
  To explore the effect of quercetin on the invasion, migration, proliferation and cell cycle of glioma U87 cells.
  
  
  METHODS
  Glioma U87 cells were treated with 50, 100, or 150 µmol/L quercetin (Q(50), Q(100) and Q(150) groups, respectively) or with DMSO (Q(0) group). Transwell in vitro invasion and migration assays, Click-iT Edu test and flow cytometry were performed to evaluate the effect of quercetin on the invasion, migration, proliferation and cell cycle of U87 cells.
  
  
  RESULTS
  After 36 h of quercetin treatment, the cells in Q(50), Q(100) and Q(150) groups showed invasive cell percentages (relative to Q(0) group) of 52.08%, 24.63%, and 13.13%, respectively (P<0.05). After quercetin treatment for 12 h, the migrating cell percentages (relative to Q(0) group) in Q(50), Q(100) and Q(150) groups were 49.46%, 26.78%, and 14.56%, respectively (P<0.05). After 24 h of quercetin treatment, the cell proliferation ratios in Q(0), Q(50), Q(100) and Q(150) groups were 25.21%, 18.38%, 16.74% and 15.24%; the cell percentages in phase G0/Gl were 71.14%, 72.71%, 69.29%, and 66.47%, phase S were 25.32%, 22.48%, 21.96%, and 23.32%, and phase G(2)/M were 3.53%, 4.80%, 8.75%, and 10.25% in the 4 groups, respectively, showing a significant difference between groups Q(100), Q(150) and group Q(0) in phase G(2)/M cell percentages (P<0.05).
  
  
  CONCLUSIONS
  Quercetin can significantly inhibit the invasion, migration and proliferation of glioma U87 cells by blocking the cell cycle progression.
authors: Southern Medical University, Guangzhou, China. yuanzhaohu@qq.com
authors: Zhaohu
authors: Z
authors: Yuan
authors: Ziyou
authors: Z
authors: Hu
authors: Lanlan
authors: L
authors: Zhang
authors: Xiaohui
authors: X
authors: Yan
authors: Huili
authors: H
authors: Wang
authors: Bingyi
authors: B
authors: Wu
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D011794:Quercetin