title: HLA class II SNP interactions and the association with type 1 diabetes mellitus in Bengali speaking patients of Eastern India.
abstract: BACKGROUND
  Several studies have demonstrated a fundamental role for the HLA in the susceptibility of, or protection to, type 1 diabetes mellitus (T1DM). However, this has not been adequately studied in Asian Indian populations. To assess the frequency of HLA class II (DPA1, DPB1, DQA1, DQB1 and DRB1) associated to susceptibility or protection toT1DM in a Bengali population of India with diabetes.
  
  
  RESULTS
  Single nucleotide polymorphism study. The HLA genotyping was performed by a polymerase chain reaction followed by their HLA-DP, DQ, and DRB1 genotypes and haplotypes by sequencing method. The results are studied by Plink software. The χ2 tests were used for the inferential statistics. To our knowledge, this study is the first of a kind which has attempted to check the HLA association with T1DM by SNPs analysis. The study recruited 151 patients with T1DM and same number of ethno-linguistic, sex matched non-diabetic controls. The present study found a significant SNP rs7990 of HLA-DQA1 (p = 0.009) negative correlation, again indicating that risk from HLA is considerably more with T1DM.
  
  
  CONCLUSIONS
  This study demonstrates that the HLA class-II alleles play a major role in genetic basis of T1DM.
authors: Anthropological Survey of India, Kolkata, West Bengal 700016, India.
authors: Oindrila
authors: O
authors: Raha
authors: Biswanath
authors: B
authors: Sarkar
authors: Bhaskar V K S
authors: BV
authors: Lakkakula
authors: Veerraju
authors: V
authors: Pasumarthy
authors: Sudhakar
authors: S
authors: Godi
authors: Subhankar
authors: S
authors: Chowdhury
authors: Pradip
authors: P
authors: Raychaudhuri
authors: Raghavendra Rao
authors: RR
authors: Vadlamudi
mesh_terms: D000328:Adult; D000483:Alleles; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000473:pathology; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D006682:HLA-DP Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D059811:HLA-DRB1 Chains