title: Novel description of mcl-PHA biosynthesis by Pseudomonas chlororaphis from animal-derived waste.
abstract: A novel description of mcl-PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (μmax.) of 0.08 h(-1), 0.10 h(-1) and 0.13 h(-1), respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum.
authors: Graz University of Technology, Institute of Biotechnology and Biochemical Engineering, Petersgasse 12, 8010, Graz, Austria.
authors: Alexander
authors: A
authors: Muhr
authors: Eva Maria
authors: EM
authors: Rechberger
authors: Anna
authors: A
authors: Salerno
authors: Angelika
authors: A
authors: Reiterer
authors: Karin
authors: K
authors: Malli
authors: Katharina
authors: K
authors: Strohmeier
authors: Sigurd
authors: S
authors: Schober
authors: Martin
authors: M
authors: Mittelbach
authors: Martin
authors: M
authors: Koller
mesh_terms: D056804:Biofuels; D002210:Caprylates / Q000737:chemistry / Q000378:metabolism; D005223:Fats / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism*
chemical_list: D056804:Biofuels; D002210:Caprylates; D005223:Fats; D054813:Polyhydroxyalkanoates; C101940:3-hydroxyoctanoic acid; C013698:tallow