title: Generation and epitope mapping of a monoclonal antibody against nucleoprotein of Ebola virus.
abstract: Ebola virus (EBOV) causes highly lethal hemorrhagic fever in humans and nonhuman primates and has a significant impact on public health. The nucleoprotein (NP) of EBOV (EBOV-NP) plays a central role in virus replication and has been used as a target molecule for disease diagnosis. In this study, we generated a monoclonal antibody (MAb) against EBOV-NP and mapped the epitope motif required for recognition by the MAb. The MAb generated via immunization of mice with prokaryotically expressed recombinant NP of the Zaire Ebola virus (ZEBOV-NP) was specific to ZEBOV-NP and able to recognize ZEBOV-NP expressed in prokaryotic and eukaryotic cells. The MAb cross-reacted with the NP of the Reston Ebola virus (REBOV), the Cote-d'Ivoire Ebola virus (CIEBOV) and the Bundibugyo Ebola virus (BEBOV) but not with the NP of the Sudan Ebola virus (SEBOV) or the Marburg virus (MARV). The minimal epitope sequence required for recognition by the MAb was the motif PPLESD, which is located between amino acid residues 583 and 588 at the C-terminus of ZEBOV-NP and well conserved among all 16 strains of ZEBOV, CIEBOV and BEBOV deposited in GenBank. The epitope motif is conserved in four out of five strains of REBOV.
authors: Forestry and Biotechnology School, Zhejiang Agriculture and Forestry University, Lin'an 311300, Zhejiang, China. xiaoduwang@163.com
authors: Xiaodu
authors: X
authors: Wang
authors: Yang
authors: Y
authors: Liu
authors: Haoting
authors: H
authors: Wang
authors: Zixue
authors: Z
authors: Shi
authors: Fanfan
authors: F
authors: Zhao
authors: Jianchao
authors: J
authors: Wei
authors: Donghua
authors: D
authors: Shao
authors: Zhiyong
authors: Z
authors: Ma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D029043:Ebolavirus / Q000737:chemistry / Q000276:immunology*; D018604:Epitope Mapping / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009698:Nucleoproteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D009698:Nucleoproteins; D011994:Recombinant Proteins