title: Battery-triggered ultrasensitive electrochemiluminescence detection on microfluidic paper-based immunodevice based on dual-signal amplification strategy.
abstract: Dual-signal amplification strategy for ultrasensitive electrochemiluminescence (ECL) multiplexed immunoassay on microfluidic paper-based analytical devices (μ-PADs) was demonstrated. This dual-signal amplification technique was achieved by employing graphene oxide-chitosan/gold nanoparticles (GCA) immunosensing platform and [4,4'-(2,5-dimethoxy-1,4-phenylene)bis(ethyne-2,1-diyl) dibenzoic acid] (P-acid) functionalized nanoporous silver (P-acid/NPS) signal amplification label. For further low-cost and disposable applications, battery-triggered constant-potential ECL (+1.0V for P-acid label (vs. Ag/AgCl auxiliary electrode)) was applied on this paper-based immunodevice with the aid of a home-made voltage-tunable power device, allowing the traditional electrochemical workstation to be abandoned. We found that two tumor markers could be sequentially detected in the linear ranges of 0.003-20 and 0.001-10ngmL(-1) with the detection limits down to 1.0 and 0.8pgmL(-1), respectively, by simply reversing the connection mode on two working electrodes. The results exhibited excellent precision and high sensitivity of such immunoassay, and it also demonstrated that this battery-triggered ECL paper-based immunodevice could provide a rapid, simple and simultaneous multiplex immunoassay with high throughput, low-cost and low detection limits for point-of-care testing.
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors: Weiping
authors: W
authors: Li
authors: Meng
authors: M
authors: Li
authors: Shenguang
authors: S
authors: Ge
authors: Mei
authors: M
authors: Yan
authors: Jiadong
authors: J
authors: Huang
authors: Jinghua
authors: J
authors: Yu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D010087:Oxides / Q000737:chemistry; D010209:Paper; D017430:Prostate-Specific Antigen / Q000032:analysis*; D012834:Silver / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D010087:Oxides; D012834:Silver; D006046:Gold; D006108:Graphite; D048271:Chitosan; D017430:Prostate-Specific Antigen