title: Pyrvinium pamoate does not activate protein kinase CK1, but promotes Akt/PKB down-regulation and GSK3 activation.
abstract: It has been reported that pyrvinium pamoate (PyrPam), an FDA (U.S. Food and Drug Administration)-approved anthelminthic drug, is a potent inhibitor of Wnt signalling by a mechanism which implies the direct activation of protein kinase CK1α. In the present paper, we provide data ruling out any direct stimulatory effect of PyrPam on CK1, by showing that the catalytic activity of CK1α and those of its isoforms δ and γ1 are not significantly affected by PyrPam when tested with the aid of specific peptide and protein substrates. Accordingly, cell treatment with PyrPam has no significant effect on the phosphorylation of β-catenin Ser(45). By contrast, the phosphorylation of β-catenin Thr(41) is increased upon cell treatment with PyrPam, through a mechanism that implies the upstream dephosphorylation of Akt/PKB (protein kinase B) and of GSK3 (glycogen synthase kinase 3). It can be concluded from the present study that PyrPam is not a bona fide activator of CK1, its perturbation of cell signalling pathways being mediated by a complex mechanism initiated by a fall in Akt phosphorylation whose down-regulation promotes reduced phosphorylation and activation of GSK3. Consistent with this, lysates of cells treated with PyrPam display enhanced protein phosphorylation which is unaffected by CK1 inhibition, while disappearing upon inhibition of GSK3. Our data are consistent with the observation that PyrPam ultimately inhibits Wnt signalling despite its lack of efficacy on CK1.
authors: Department of Biomedical Sciences and CNR Institute of Neurosciences, University of Padova, Viale G. Colombo 3, 35131 Padova, Italy.
authors: Andrea
authors: A
authors: Venerando
authors: Cristina
authors: C
authors: Girardi
authors: Maria
authors: M
authors: Ruzzene
authors: Lorenzo A
authors: LA
authors: Pinna
mesh_terms: D000818:Animals; D047389:Casein Kinase I / Q000378:metabolism*; D006224:Cricetinae; D015536:Down-Regulation / Q000187:drug effects* / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011774:Pyrvinium Compounds / Q000494:pharmacology*
chemical_list: D011774:Pyrvinium Compounds; C024631:pyrvinium; D047389:Casein Kinase I; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3