title: Prediction and characterization of the linear IgE epitopes for the major soybean allergen β-conglycinin using immunoinformatics tools.
abstract: The α subunit of β-conglycinin is a major allergen in soybean. The objective of this study was to predict and identify the linear immunoglobulin (Ig)E epitopes of the soybean α subunit of β-conglycinin. Three immunoinformatics tools were used to predict the potential epitopes and were confirmed by dot-blot inhibition using sera from soybean allergic subjects. As a result, 15 peptides were predicted and assembled by solid-phase synthesis. Eleven epitopes were identified by the dot-blot inhibition test. Moreover, peptide 3 had IgE binding capability with all sera(5/5) tested, while peptide 1, 4, 6, 8 and12 could bind to 4/5 of the sera samples. Secondary structure prediction of peptide 3 and circular dichroism test validated that the structure of peptide 3 was a random coil.
authors: State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Wuxi, Jiangsu 214122, China. sxlzzz@jiangnan.edu.cn
authors: Xiulan
authors: X
authors: Sun
authors: Xiaohong
authors: X
authors: Shan
authors: Zihe
authors: Z
authors: Yan
authors: Yinzhi
authors: Y
authors: Zhang
authors: Lu
authors: L
authors: Guan
mesh_terms: D000328:Adult; D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005260:Female; D005916:Globulins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D015203:Reproducibility of Results; D055314:Seed Storage Proteins / Q000737:chemistry / Q000276:immunology*; D060327:Solid-Phase Synthesis Techniques; D030262:Soybean Proteins / Q000737:chemistry / Q000276:immunology*; D013025:Glycine max / Q000737:chemistry / Q000276:immunology*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D000939:Epitopes; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D007073:Immunoglobulin E