title: Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.
abstract: Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.
authors: Department of Environmental Microbiology, Helmholtz Centre for Environmental Research, Leipzig, Germany.
authors: Julia
authors: J
authors: Giebler
authors: Lukas Y
authors: LY
authors: Wick
authors: Michael
authors: M
authors: Schloter
authors: Hauke
authors: H
authors: Harms
authors: Antonis
authors: A
authors: Chatzinotas
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D042926:Cytochrome P-450 CYP4A / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil; D042926:Cytochrome P-450 CYP4A