title: Reagentless D-sorbitol biosensor based on D-sorbitol dehydrogenase immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite.
abstract: A reagentless D-sorbitol biosensor based on NAD-dependent D-sorbitol dehydrogenase (DSDH) immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite has been developed. It was prepared by durably immobilizing the NAD(+) cofactor with DSDH in a sol-gel thin film on the surface of carbon nanotubes functionalized with poly(methylene green). This device enables selective determination of D-sorbitol at 0.2 V with a sensitivity of 8.7 μA mmol(-1) L cm(-2) and a detection limit of 0.11 mmol L(-1). Moreover, this biosensor has excellent operational stability upon continuous use in hydrodynamic conditions.
authors: Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, UMR 7564, CNRS-Université de Lorraine and CNRS, 405, rue de Vandoeuvre, 54600 Villers-lès-Nancy, France.
authors: Zhijie
authors: Z
authors: Wang
authors: Mathieu
authors: M
authors: Etienne
authors: Veronika
authors: V
authors: Urbanova
authors: Gert-Wieland
authors: GW
authors: Kohring
authors: Alain
authors: A
authors: Walcarius
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D007064:L-Iditol 2-Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D057230:Limit of Detection; D008751:Methylene Blue / Q000031:analogs & derivatives* / Q000737:chemistry; D009243:NAD / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry*; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry; D013012:Sorbitol / Q000032:analysis* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011108:Polymers; D009243:NAD; D013012:Sorbitol; D007064:L-Iditol 2-Dehydrogenase; C028673:methylene green; D008751:Methylene Blue