title: Autofluorescence generation and elimination: a lesson from glutaraldehyde.
abstract: Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.
authors: Department of Chemistry Education, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul 151-742, South Korea.
authors: Kwahun
authors: K
authors: Lee
authors: Sungmoon
authors: S
authors: Choi
authors: Chun
authors: C
authors: Yang
authors: Hai-Chen
authors: HC
authors: Wu
authors: Junhua
authors: J
authors: Yu
mesh_terms: D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D005029:Ethylenediamines / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D000588:Amines; D002232:Carbocyanines; D005029:Ethylenediamines; D010455:Peptides; D011506:Proteins; C089187:cyanine dye 3; C031234:ethylenediamine; D005976:Glutaral