title: A dual mode pulsed electro-magnetic cell stimulator produces acceleration of myogenic differentiation.
abstract: This paper presents the design and test of a dual-mode electric and magnetic biological stimulator (EM-Stim). The stimulator generates pulsing electric and magnetic fields at programmable rates and intensities. While electric and magnetic stimulators have been reported before, this is the first device that combines both modalities. The ability of the dual stimulation to target bone and muscle tissue simultaneously has the potential to improve the therapeutic treatment of osteoporosis and sarcopenia. The device is fully programmable, portable and easy to use, and can run from a battery or a power supply. The device can generate magnetic fields of up to 1.6 mT and output voltages of +/- 40 V. The EM-Stim accelerated myogenic differentiation of myoblasts into myotubes as evidenced by morphometric, gene expression, and protein content analyses. Currently, there are many patents concerned with the application of single electrical or magnetic stimulation, but none that combine both simultaneously. However, we applied for and obtained a provisional patent for new device to fully explore its therapeutic potential in pre-clinical models.
authors: Muscle Biology Research Group, Schools of Nursing & Medicine, University of Missouri Kansas City, 2464 Charlotte Street, Kansas City, MO 64108. brottom@umkc.edu.
authors: Walter D
authors: WD
authors: Leon-Salas
authors: Hatem
authors: H
authors: Rizk
authors: Chenglin
authors: C
authors: Mo
authors: Noah
authors: N
authors: Weisleder
authors: Leticia
authors: L
authors: Brotto
authors: Eduardo
authors: E
authors: Abreu
authors: Marco
authors: M
authors: Brotto
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002460:Cell Line; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods*; D004558:Electric Stimulation / Q000295:instrumentation*; D004574:Electromagnetic Fields; D006801:Humans; D051379:Mice; D024510:Muscle Development / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology*; D010330:Patents as Topic