title: Thermophilic archaeal community succession and function change associated with the leaching rate in bioleaching of chalcopyrite.
abstract: The community succession and function change of thermophilic archaea Acidianus brierleyi, Metallosphaera sedula, Acidianus manzaensis and Sulfolobus metallicus were studied by denaturing gradient gel electrophoresis (DGGE) analysis of amplifying 16S rRNA genes fragments and real-time qPCR analysis of amplifying sulfur-oxidizing soxB gene associated with chalcopyrite bioleaching rate at different temperatures and initial pH values. The analysis results of the community succession indicated that temperature and initial pH value had a significant effect on the consortium, and S. metallicus was most sensitive to the environmental change, A. brierleyi showed the best adaptability and sulfur oxidation ability and predominated in various leaching systems. Meanwhile, the leaching rate of chalcopyrite closely related to the consortium function embodied by soxB gene, which could prove a desirable way for revealing microbial sulfur oxidation difference and tracking the function change of the consortium, and for optimizing the leaching parameters and improving the recovery of valuable metals.
authors: School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China.
authors: Wei
authors: W
authors: Zhu
authors: Jin-lan
authors: JL
authors: Xia
authors: Yi
authors: Y
authors: Yang
authors: Zhen-yuan
authors: ZY
authors: Nie
authors: An-an
authors: AA
authors: Peng
authors: Hong-chang
authors: HC
authors: Liu
authors: Guan-zhou
authors: GZ
authors: Qiu
mesh_terms: D001105:Archaea / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003300:Copper / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D058645:Denaturing Gradient Gel Electrophoresis; D019848:Gene Expression Regulation, Archaeal; D019766:Genes, Archaeal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C012819:chalcopyrite; D003300:Copper