title: A Y2H-seq approach defines the human protein methyltransferase interactome.
abstract: To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.
authors: Otto-Warburg Laboratory, Max Planck Institute for Molecular Genetics (MPIMG), Berlin, Germany.
authors: Mareike
authors: M
authors: Weimann
authors: Arndt
authors: A
authors: Grossmann
authors: Jonathan
authors: J
authors: Woodsmith
authors: Ziya
authors: Z
authors: Özkan
authors: Petra
authors: P
authors: Birth
authors: David
authors: D
authors: Meierhofer
authors: Nouhad
authors: N
authors: Benlasfer
authors: Taras
authors: T
authors: Valovka
authors: Bernd
authors: B
authors: Timmermann
authors: Erich E
authors: EE
authors: Wanker
authors: Sascha
authors: S
authors: Sauer
authors: Ulrich
authors: U
authors: Stelzl
mesh_terms: D002853:Chromatography, Liquid; D004926:Escherichia coli; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D053719:Tandem Mass Spectrometry; D020798:Two-Hybrid System Techniques
chemical_list: D008780:Methyltransferases