title: Antiplatelet activity of a novel formula composed of malic acid, succinic acid and citric acid from Cornus officinalis fruit.
abstract: The present study investigated the antiplatelet activity of a novel formula composed by malic acid, succinic acid and citric acid with a ratio of 3:2:2. The IC50 and inhibition of platelet aggregation induced by various agonists as well as platelet adhesion were evaluated in vitro. Of note, the IC50 for the formula inhibiting adenosine diphosphate (ADP)-induced platelet aggregation was 0.185 mg/mL. Meanwhile, the formula showed more potent inhibitory effect on platelet aggregation induced by ADP and thrombin than the single component at same concentration (0.37 mg/mL). Moreover, the formula could prevent platelet adhesion significantly without influence on platelet viability.
authors: Department of clinic pharmacology, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210029, P.R. China.
authors: Qi-Chun
authors: QC
authors: Zhang
authors: Yue
authors: Y
authors: Zhao
authors: Hui-Min
authors: HM
authors: Bian
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D019343:Citric Acid / Q000494:pharmacology*; D029768:Cornus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008293:Malates / Q000494:pharmacology*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011817:Rabbits; D019802:Succinic Acid / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology
chemical_list: D008293:Malates; D010975:Platelet Aggregation Inhibitors; D019343:Citric Acid; D000244:Adenosine Diphosphate; C030298:malic acid; D019802:Succinic Acid; D013917:Thrombin
keywords: antiplatelet activity; citric acid; formula; malic acid; succinic acid