title: Design and selection of soy breads used for evaluating isoflavone bioavailability in clinical trials.
abstract: To modulate isoflavone aglycone composition within a soy functional food, soy ingredients were processed and evaluated in a soy bread system intended for clinical trials. A soy flour/soy milk mixture (SM) was boiled, fermented, steamed, or roasted prior to dough preparation. The isoflavone compositions of five processed SM and their corresponding breads combined with and without β-glucosidase-rich almonds were examined using HPLC. Isoflavone malonyl-glucosides (>80%) were converted into acetyl and simple glucoside forms (substrates more favorable for β-glucosidase) in steamed and roasted SM. Their corresponding breads had isoflavones predominately as aglycones (∼75%) with soy-almond bread with steamed SM being more consumer acceptable than roasted. Isoflavone composition in soy bread was stable during frozen storage and toasting. A suitable glycoside-rich soy bread (31.6 ± 2.1 mg aglycone equiv/slice) using unprocessed SM and an aglycone-rich soy-almond bread (31.1 ± 1.9 mg aglycone equiv/slice) using steamed SM were developed to evaluate fundamental questions of isoflavone bioavailability in clinical trials.
authors: Department of Food Science and Technology, The Ohio State University, 110 Parker Building, 2015 Fyffe Court, Columbus, Ohio 43210, USA.
authors: Jennifer H
authors: JH
authors: Ahn-Jarvis
authors: Kenneth M
authors: KM
authors: Riedl
authors: Steven J
authors: SJ
authors: Schwartz
authors: Yael
authors: Y
authors: Vodovotz
mesh_terms: D001682:Biological Availability; D001939:Bread / Q000032:analysis*; D004355:Drug Stability; D005511:Food Handling; D005519:Food Preservation; D005615:Freezing; D006358:Hot Temperature; D006801:Humans; D007529:Isoflavones / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D027861:Prunus / Q000201:enzymology; D012639:Seeds / Q000201:enzymology; D012677:Sensation; D045730:Soy Foods / Q000032:analysis*; D041942:Soy Milk; D013227:Steam; D001617:beta-Glucosidase / Q000032:analysis / Q000378:metabolism
chemical_list: D007529:Isoflavones; D013227:Steam; D001617:beta-Glucosidase