title: Human papilloma virus 18 detection in oral squamous cell carcinoma and potentially malignant lesions using saliva samples.
abstract: BACKGROUND
  Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is one of the risk factors for developing oral cancer. For this study HPV18 was chosen as it is one of the high risk HPV types and may lead to carcinogenesis. However, prevalence of HPV18 infection in Oral Squamous Cell Carcinoma in Malaysia remains unclear.
  
  
  OBJECTIVE
  This study aimed to investigate the viral load of HPV18 DNA in OSCC and potentially malignant lesions using saliva samples.
  
  
  METHODS
  Genomic DNAs of thirty saliva samples of normal subjects and thirty saliva samples compromised of 16 samples from potentially malignant lesions and 14 of OSCC patients were amplified for HPV18 DNA using a nested polymerase chain reaction analysis. All PCR products were then analyzed using the Bioanalyzer to confirm presence of HPV18 DNA.
  
  
  RESULTS
  From thirty patients examined, only one of 30 (3.3%) cases was found to be positive for HPV18 in this study.
  
  
  CONCLUSIONS
  The finding of this study revealed that there is a low viral detection of HPV18 in Malaysian OSCC by using saliva samples, suggesting that prevalence of HPV18 may not be important in this group of Malaysian OSCC.
authors: Centre of Studies for Preclinical Science, Faculty of Dentistry, Universiti Teknologi MARA, Shah Alam, Malaysia. gootheah@salam.uitm.edu.my
authors: Khor
authors: K
authors: Goot-Heah
authors: Thong
authors: T
authors: Kwai-Lin
authors: Gabriele Ruth Anisah
authors: GR
authors: Froemming
authors: Mannil Thomas
authors: MT
authors: Abraham
authors: Nik Mohd Mazuan
authors: NM
authors: Nik Mohd Rosdy
authors: Rosnah Binti
authors: RB
authors: Zain
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology; D012463:Saliva / Q000737:chemistry
chemical_list: D004279:DNA, Viral