title: Evaluation of pyroptosis in macrophages using cytosolic delivery of purified flagellin.
abstract: Pyroptosis is a molecularly controlled form of cell death that exhibits some features of apoptosis as well of necrosis. Pyroptosis is induced by inflammasome-activated caspase-1 or caspase-11 (caspase-4 in humans), as a result of distinct pathogenic or damage stimuli. Although pyroptosis displays some morphological and biochemical features of apoptosis, it has an inflammatory outcome due to the loss of plasma membrane integrity and the consequent release of intracellular contents, reminiscent to necrosis. Here, we use cytosolic delivery of purified flagellin as an experimental tool to trigger pyroptosis and describe potential methods to study this form of cell death. Finally, we discuss the advantages and limitations of these methods.
authors: Centro de Terapia Celular e Molecular (CTC-Mol), Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Silvia L
authors: SL
authors: Lage
authors: Gustavo P
authors: GP
authors: Amarante-Mendes
authors: Karina R
authors: KR
authors: Bortoluci
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001412:Bacillus subtilis / Q000737:chemistry; D020170:Caspase 1 / Q000172:deficiency / Q000235:genetics; D003600:Cytosol / Q000378:metabolism* / Q000648:ultrastructure; D004789:Enzyme Activation; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005408:Flagellin / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009336:Necrosis / Q000235:genetics / Q000473:pathology; D021381:Protein Transport; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015398:Signal Transduction
chemical_list: D005229:Fatty Acids, Monounsaturated; D058847:Inflammasomes; D000644:Quaternary Ammonium Compounds; D005408:Flagellin; D020170:Caspase 1; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane