title: Immunogenicity and cross-reactivity against Mycobacterium tuberculosis of proteoliposomes derived from Mycobacterium bovis BCG.
abstract: The only currently available vaccine against tuberculosis (TB) is Mycobacterium bovis Bacille Calmette-Guerin (BCG), which has inconsistent efficacy to protect against the disease in adults. M. tuberculosis (MTB) cell wall components have been implicated in the pathogenicity of TB and therefore have been a prime target for the identification and characterization of cell wall proteins with potential application in vaccine development. In this regard, proteoliposomes (PLs) derived from mycobacteria containing lipids and cell wall proteins could be potential vaccine candidates against TB. In the present study PLs derived from BCG were prepared. These homogeneous population of spherical microparticles was then immunized into Balb/c mice. Sera of immunized animals showed high IgG response and strong cross-reactivity against different MTB antigens.These results showed that BCG PLs could be potential vaccine candidates against TB.
authors: Finlay Institute. Ave. 27 No. 19805, Havana, Cuba. AP. 16017, CP11600.
authors: Fátima
authors: F
authors: Reyes
authors: Yanely
authors: Y
authors: Tirado
authors: Alina
authors: A
authors: Puig
authors: Reinier
authors: R
authors: Borrero
authors: Giselle
authors: G
authors: Reyes
authors: Sonsire
authors: S
authors: Fernández
authors: José Luis
authors: JL
authors: Pérez
authors: Ramlah
authors: R
authors: Kadir
authors: Caridad
authors: C
authors: Zayas
authors: Mohd Nor
authors: MN
authors: Norazmi
authors: María E
authors: ME
authors: Sarmiento
authors: Armando
authors: A
authors: Acosta
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001500:BCG Vaccine / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D003429:Cross Reactions; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D008563:Membrane Lipids / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011510:Proteolipids / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D008563:Membrane Lipids; D011510:Proteolipids; C020485:proteoliposomes