title: Molecular cloning and characterization of major vault protein of Echinococcus multilocularis.
abstract: The cDNA clone coding a major vault protein (MVP)-like protein was derived from Echinococcus multilocularis cysts. MVP is a main component of vault particles, which are the largest cytoplasmic ribonucleoprotein particles in eukaryotic cells. We sequenced and characterized E. multilocularis MVP (EmMVP). The nucleotide sequence of the emmvp cDNA clone was 2607 bp in the full length open reading frame and its deduced amino acid sequence had several signature motifs which were specific to MVP families. Immunoblot analysis with mouse anti-EmMVP antiserum revealed that crude antigens of E. multilocularis included EmMVP protein. Furthermore, our results showed that the expression of EmMVP protein in an Sf9 insect cell line using a baculovirus vector directed the formation of particles that shared similar biochemical characteristics with other vault proteins and the distinct vault-like morphology when negatively stained and examined by electron microscopy.
authors: Hokkaido Institute of Public Health, Sapporo 060-0819, Japan. goto_a@iph.pref.hokkaido.jp
authors: Akiko
authors: A
authors: Goto
authors: Hirokazu
authors: H
authors: Kouguchi
authors: Kimiaki
authors: K
authors: Yamano
authors: Yukiharu
authors: Y
authors: Sawada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004443:Echinococcosis / Q000276:immunology; D048210:Echinococcus multilocularis / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D061987:Sf9 Cells; D020394:Vault Ribonucleoprotein Particles / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D011994:Recombinant Proteins; D020394:Vault Ribonucleoprotein Particles