title: A (S)TEM gas cell holder with localized laser heating for in situ experiments.
abstract: The advent of aberration correction for transmission electron microscopy has transformed atomic resolution imaging into a nearly routine technique for structural analysis. Now an emerging frontier in electron microscopy is the development of in situ capabilities to observe reactions at atomic resolution in real time and within realistic environments. Here we present a new in situ gas cell holder that is designed for compatibility with a wide variety of sample type (i.e., dimpled 3-mm discs, standard mesh grids, various types of focused ion beam lamellae attached to half grids). Its capabilities include localized heating and precise control of the gas pressure and composition while simultaneously allowing atomic resolution imaging at ambient pressure. The results show that 0.25-nm lattice fringes are directly visible for nanoparticles imaged at ambient pressure with gas path lengths up to 20 μm. Additionally, we quantitatively demonstrate that while the attainable contrast and resolution decrease with increasing pressure and gas path length, resolutions better than 0.2 nm should be accessible at ambient pressure with gas path lengths less than the 15 μm utilized for these experiments.
authors: Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA. smehraeen@gmail.com
authors: Shareghe
authors: S
authors: Mehraeen
authors: Joseph T
authors: JT
authors: McKeown
authors: Pushkarraj V
authors: PV
authors: Deshmukh
authors: James E
authors: JE
authors: Evans
authors: Patricia
authors: P
authors: Abellan
authors: Pinghong
authors: P
authors: Xu
authors: Bryan W
authors: BW
authors: Reed
authors: Mitra L
authors: ML
authors: Taheri
authors: Paul E
authors: PE
authors: Fischione
authors: Nigel D
authors: ND
authors: Browning
mesh_terms: D005740:Gases; D006361:Heating; D007834:Lasers; D007854:Lead / Q000032:analysis; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation* / Q000379:methods*; D053758:Nanoparticles / Q000032:analysis*; D010087:Oxides / Q000032:analysis; D014025:Titanium / Q000032:analysis
chemical_list: D005740:Gases; D010087:Oxides; C009495:titanium dioxide; D007854:Lead; C047365:lead oxide; D014025:Titanium