title: Spontaneous release of bacteriophage particles by Lactobacillus rhamnosus pen.
abstract: The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.
authors: Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Sciences in Lublin, 20-704 Lublin, Poland.
authors: Piotr
authors: P
authors: Jarocki
authors: Marcin
authors: M
authors: Podleśny
authors: Jarosław
authors: J
authors: Pawelec
authors: Agata
authors: A
authors: Malinowska
authors: Sylwia
authors: S
authors: Kowalczyk
authors: Zdzisław
authors: Z
authors: Targoński
mesh_terms: D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005814:Genes, Viral; D052201:Lacticaseibacillus rhamnosus / Q000821:virology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D039002:Prophages / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000032:analysis; D017376:Viral Tail Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D017376:Viral Tail Proteins