title: Evaluation of mucoadhesive carrier adjuvant: toward an oral anthrax vaccine.
abstract: The aim of present study was to evaluate the potential of mucoadhesive alginate-coated chitosan microparticles (A-CHMp) for oral vaccine against anthrax. The zeta potential of A-CHMp was -29.7 mV, and alginate coating could prevent the burst release of antigen in simulated gastric fluid. The results indicated that A-CHMp was mucoadhesive in nature and transported it to the peyer's patch upon oral delivery. The immunization studies indicated that A-CHMp resulted in the induction of potent systemic and mucosal immune responses, whereas alum-adjuvanted rPA could induce only systemic immune response. Thus, A-CHMp represents a promising acid carrier adjuvant for oral immunization against anthrax.
authors: Department of Pharmaceutical Sciences, Drug Delivery Research Laboratory, Dr. H. S. Gour University , Sagar, (M.P.) , India.
authors: Sharad
authors: S
authors: Mangal
authors: Dilip
authors: D
authors: Pawar
authors: Udita
authors: U
authors: Agrawal
authors: Arvind K
authors: AK
authors: Jain
authors: Suresh P
authors: SP
authors: Vyas
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry; D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry; D000818:Animals; D000881:Anthrax / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000008:administration & dosage* / Q000276:immunology; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001408:Bacillus anthracis / Q000737:chemistry / Q000276:immunology*; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000276:immunology; D040761:Biomimetic Materials / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D005260:Female; D005750:Gastric Juice / Q000737:chemistry; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010581:Peyer's Patches; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D000464:Alginates; D022122:Anthrax Vaccines; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C030325:anthrax toxin; D048271:Chitosan