title: An improved system for the evaluation of antiviral compounds against herpes simplex virus type 2.
abstract: Infection with herpes simplex virus type 2 (HSV-2) can result in lesions in reproductive organs, along with long-term latency. In this work, a non-lethal strain of HSV-2 which was isolated clinically was used to infect female mice intravaginally. Body weight, vulval lesions, histological examination of vaginal tissue, and viral load were monitored and used as indices for evaluating antiviral drugs against HSV-2 infection. The results indicated that mice infected with HSV-2 exhibited significant reduction in body weight, serious vulval lesions, massive lymphocyte invasion of vaginal tissue, and approximately 10⁴ copies/μl of HSV-2 were found in vaginal and uterine tissues. Aciclovir (ACV) treatment inhibited loss in body weight, genital pathology and virus replication (reduced to 10⁰·³ copies/μl) effectively. The study provides a simple, reproducible and feasible animal model for anti-HSV-2 drugs evaluation and HSV-2 vaccine research.
authors: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors: Shiyu
authors: S
authors: Yin
authors: Yongdong
authors: Y
authors: Li
authors: Han
authors: H
authors: Xia
authors: Jiuru
authors: J
authors: Zhao
authors: Zhong
authors: Z
authors: Zhang
authors: Shuang
authors: S
authors: Tang
authors: Zheng
authors: Z
authors: Kou
authors: Jing
authors: J
authors: Chen
authors: Jingfeng
authors: J
authors: Yu
authors: Zhaojun
authors: Z
authors: Fan
authors: Tianxian
authors: T
authors: Li
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage* / Q000494:pharmacology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D006558:Herpes Genitalis / Q000188:drug therapy* / Q000473:pathology*; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D016896:Treatment Outcome
chemical_list: D000998:Antiviral Agents