title: Functional inhibition of UQCRB suppresses angiogenesis in zebrafish.
abstract: As a subunit of mitochondrial complex III, UQCRB plays an important role in complex III stability, electron transport, and cellular oxygen sensing. Herein, we report UQCRB function regarding angiogenesis in vivo with the zebrafish (Danio rerio). UQCRB knockdown inhibited angiogenesis in zebrafish leading to the suppression of VEGF expression. Moreover, the UQCRB-targeting small molecule terpestacin also inhibited angiogenesis and VEGF levels in zebrafish, supporting the role of UQCRB in angiogenesis. Collectively, UQCRB loss of function by either genetic and pharmacological means inhibited angiogenesis, indicating that UQCRB plays a key role in this process and can be a prognostic marker of angiogenesis- and mitochondria-related diseases.
authors: Chemical Genomics National Research Laboratory, Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science & Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
authors: Yoon Sun
authors: YS
authors: Cho
authors: Hye Jin
authors: HJ
authors: Jung
authors: Seung Hyeok
authors: SH
authors: Seok
authors: Alexander Y
authors: AY
authors: Payumo
authors: James K
authors: JK
authors: Chen
authors: Ho Jeong
authors: HJ
authors: Kwon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D060172:Morpholinos / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000235:genetics*; D011379:Prognosis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000502:physiology*
chemical_list: D001643:Bridged Bicyclo Compounds; D002352:Carrier Proteins; D060172:Morpholinos; D042461:Vascular Endothelial Growth Factor A; C027208:ubiquinone-binding proteins; C080890:terpestacin