title: Effect of allergen inhalation on airway oxidant stress, using exhaled breath condensate 8-isoprostane, in mild asthma.
abstract: OBJECTIVE
  Exhaled breath condensate (EBC) 8-isoprostane concentrations are increased in asthma, but it is not known if they acutely change following bronchoprovocation. The objective of this study was to evaluate EBC 8-isoprostane concentrations following allergen-induced bronchoprovocation in asthma.
  
  
  METHODS
  This comparison study included eight mild atopic asthmatics and six controls. Asthmatics were challenged with inhaled specific allergen, methacholine, and irrelevant allergen in random order. Controls were challenged with irrelevant allergen. EBCs collected at 0, 3, 6, 9, and 23 hours by the R-tube method were measured for 8-isoprostanes by ELISA technique. Repeated measures ANOVA technique was used for analysis.
  
  
  RESULTS
  EBC 8-isoprostane concentrations did not change following any inhalational challenge, as compared to baseline, in either asthmatics or controls.
  
  
  CONCLUSIONS
  EBC 8-isoprostane concentrations do not acutely change following bronchoprovocation in subjects with mild asthma.
authors: Department of Medicine, University of New Mexico Health Sciences Center School of Medicine, Albuquerque, NM, USA. asood@salud.unm.edu
authors: Akshay
authors: A
authors: Sood
authors: Clifford
authors: C
authors: Qualls
authors: JeanClare
authors: J
authors: Seagrave
authors: Jacob
authors: J
authors: McDonald
authors: Rugia
authors: R
authors: Shohreh
authors: Annalisa
authors: A
authors: Chiavaroli
authors: Mark
authors: M
authors: Schuyler
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000485:Allergens / Q000276:immunology*; D001249:Asthma / Q000378:metabolism*; D001944:Breath Tests; D001980:Bronchi / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress; D011863:Radioimmunoassay
chemical_list: D000485:Allergens; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost