title: Potential of Fourier transform infrared spectroscopy (FT-IR) to differentiate environmental Aspergillus fungi species A. niger, A. ochraceus, and A. westerdijkiae using two different methodologies.
abstract: We assessed the ability of Fourier transform infrared spectroscopy (FT-IR) to differentiate three important and morphologically similar Aspergillus species: A. ochraceus and A. westerdijkiae, and A. niger. Fungi were processed by two methods, powdered mycelia and conidiospore-saline solution, and then recorded in a spectrometer. Second derivatives with nine points of smoothing were applied as spectra data pretreatment. Partial least squares regression was used for the species comparison models and a prediction test was used to evaluate the models. The powdered-mycelia methodology correctly identified 100% of the prediction test set to discriminate A. niger from A. ochraceus and A. westerdijkiae; in addition, it had a 86.6% success rate in discriminating A. ochraceus and A. westerdijkiae. This is the first time a study assessed the ability of FT-IR to differentiate A. niger, A. ochraceus, and A. westerdijkiae, and we believe this technique is very promising for classifying and distinguish fungi isolates.
authors: Basic Pathology Department, Federal University of Parana, Curitiba-PR 80010-180, Brazil.
authors: Sabina Moser
authors: SM
authors: Tralamazza
authors: Angela
authors: A
authors: Bozza
authors: João Guilherme Rodenbusch
authors: JG
authors: Destro
authors: Jaime Iván
authors: JI
authors: Rodríguez
authors: Patricia
authors: P
authors: do Rocio Dalzoto
authors: Ida Chapaval
authors: IC
authors: Pimentel
mesh_terms: D001230:Aspergillus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*; D016018:Least-Squares Analysis; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*