title: Genetic structure of the aphid, Chaetosiphon fragaefolii, and its role as a vector of the Strawberry Yellow Edge Virus to a native strawberry, Fragaria chiloensis in Chile.
abstract: The monoecious anholocyclical aphid, Chaetosiphon fragaefolii (Cockerell) (Homoptera: Aphididae), was collected on a native strawberry, Fragaria chiloensis (L.) Duchesne (Rosales: Rosaceae) from different sites in Chile. The presence of this aphid was recorded during two consecutive years. F. chiloensis plants were collected from seven natural and cultivated growing areas in central and southern Chile. Aphids were genotyped by cross-species amplification of four microsatellite loci from other aphid species. In addition, the aphid borne virus Strawberry mild yellow edge virus was confirmed in F. chiloensis plants by double-antibody sandwich ELISA and RT-PCR. Genetic variability and structure of the aphid populations was assessed from the geo-referenced individuals through AMOVA and a Bayesian assignment test. The presence of C. fragaefolii, during the two-year study was detected in only four of the seven sites (Curepto, Contulmo, Chilián and Cucao). Genetic variation among these populations reached 19% of the total variance. When assigning the individuals to groups, these were separated in three genetic clusters geographically disjunct. Of the seven sampled sites, six were positive for the virus by RT-PCR, and five by double-antibody sandwich ELISA . The incidence of the virus ranged from 0-100%. Presence of the virus corresponded with the presence of the aphid in all but two sites (Chilian and Vilches). The greatest incidence of Strawberry mild yellow edge virus was related to the abundance of aphids. On the other hand, sequences of the coat protein gene of the different virus samples did not show correspondence with either the genetic groups of the aphids or the sampling sites. The genetic structure of aphids could suggest that dispersal is mainly through human activities, and the spread to natural areas has not yet occurred on a great scale.
authors: Laboratories de Interacciones Insecto-Planta, Instituto de Biología y Biotecnología Vegetal, Universidad de Talca, 2 Norte 685, Talca, Chile. blavandero@utalca.cl
authors: Blas
authors: B
authors: Lavandero
authors: Pamela
authors: P
authors: Rojas
authors: Claudio C
authors: CC
authors: Ramirez
authors: Marcela
authors: M
authors: Salazar
authors: Peter D S
authors: PD
authors: Caligari
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001042:Aphids / Q000235:genetics* / Q000502:physiology / Q000821:virology*; D001499:Bayes Theorem; D002677:Chile; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020387:Food Chain; D031985:Fragaria / Q000254:growth & development / Q000821:virology*; D051456:Gene Flow; D017344:Genes, Insect; D014644:Genetic Variation; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction; D017863:Potexvirus / Q000235:genetics*; D012621:Seasons