title: Rapid identification of Vibrio parahaemolyticus isolated from shellfish, sea water and sediments of the Khnifiss lagoon, Morocco, by MALDI-TOF mass spectrometry.
abstract: We establish the presence of Vibrio parahaemolyticus and deepen the comparison of isolates using MALDI-TOF MS for the typing of isolates originating from the Khnifiss lagoon (Morocco). Amongst 48 samples from sea water, sediment and shellfish isolated from different sites of Khnifiss lagoon, Morocco, we obtained 22 isolates of V. parahaemolyticus identified by Vitek 2™ System (bioMérieux) and MALDI Biotyper™ (Bruker Daltonics). All isolates were highly resistant to ampicillin and ticarcillin, moderately resistant to cefalotin, but sensitive to 16 other antimicrobials tested. MALDI-TOF MS was used to discriminate between closely related environmental strains of V. parahaemolyticus. A clustering and distribution based on MALDI-TOF spectra were generated using the BioTyper 1.1™ software. Despite low diversity in regard to the biochemical characteristics and antimicrobial resistance, the isolates evoke a larger biodiversity when analysed through mass spectra of abundant proteins. Different evaluations of a cut-off value showed that, when placed at a 10% threshold of the whole diversity, isolates differed by at least three mass peaks.
authors: Faculty of Sciences, Laboratory of Biotechnology & Valorisation of Natural Resources, University Ibn Zohr, Agadir, Morocco. eaudesyeux@yahoo.fr
authors: S M
authors: SM
authors: Malainine
authors: W
authors: W
authors: Moussaoui
authors: G
authors: G
authors: Prévost
authors: J-M
authors: JM
authors: Scheftel
authors: R
authors: R
authors: Mimouni
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D019015:Geologic Sediments / Q000382:microbiology*; D009018:Morocco; D010449:Peptide Mapping; D012623:Seawater / Q000382:microbiology*; D012758:Shellfish / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014736:Vibrio parahaemolyticus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins