title: [Nano-Se-chondroitin sulfate inhibits T-2 toxin-induced apoptosis of cultured chondrocytes from patients with Kashin-Beck disease].
abstract: OBJECTIVE
  To observe the effect of nano-Se-chondroitin sulfate on the growth and apoptosis of chondrocytes from patients with Kashin-Beck disease (KBD) exposed to T-2 toxin in vitro.
  
  
  METHODS
  Samples of the articular cartilage were obtained from 6 patients with grade II/III KBD diagnosed in line with the National Clinical Diagnostic Criteria of KBD (WS/T 207-2010) for chondrocyte separation and culture in vitro. The separated chondrocytes were treated with synthesized nano-Se-chondroitin sulfate particles and T-2 toxin, alone or in combination, and the cell growth and apoptosis were observed using MTT assay, HE staining and flow cytometry.
  
  
  RESULTS
  The synthesized nano-Se-chondroitin sulfate, with a selenium entrapment ratio of 10.1%, spontaneously formed nanoparticles in distilled water with sizes ranging from 30 to 200 nm. Fourier-transform infrared spectroscopy suggested a possible covalent bond that bound Nano-Se and chondroitin sulfate. Within the concentration range of 50-200 ng/ml, nano-Se-chondroitin sulfate significantly inhibited T-2 toxin-induced apoptosis of the cultured chondrocytes and reduced the early apoptosis rate to (8.64∓1.57)% (P<0.05).
  
  
  CONCLUSIONS
  Nano-Se-chondroitin sulfate can inhibit T-2 toxin-induced apoptosis of cultured chondrocytes from KBD patients in vitro, and serves as a promising candidate therapeutic agent for KBD.
authors: Xi'an Jiaotong University College of Medicine, Xi'an, China. bbbishop@126.com
authors: Jing
authors: J
authors: Han
authors: Xiong
authors: X
authors: Guo
authors: Cuiyan
authors: C
authors: Wu
authors: Chunyan
authors: C
authors: Li
authors: Shulan
authors: S
authors: He
authors: Chen
authors: C
authors: Duan
authors: Yujie
authors: Y
authors: Ning
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology*; D002809:Chondroitin Sulfates / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D057767:Kashin-Beck Disease / Q000473:pathology*; D008875:Middle Aged; D049329:Nanostructures; D013605:T-2 Toxin / Q000633:toxicity*
chemical_list: D002809:Chondroitin Sulfates; D013605:T-2 Toxin