title: The effect of subcutaneous mesenchymal stem cell injection on statis zone and apoptosis in an experimental burn model.
abstract: BACKGROUND
  In an acute burn injury the zone of stasis is initially vital but may progress to coagulation necrosis with time. In this study, salvage of the zone of stasis was aimed at by subcutaneous mesenchymal stem cell injection.
  
  
  METHODS
  Mesenchymal stem cells were obtained from the bone marrow of Sprague-Dawley rats (n = 10). Twenty Sprague-Dawley rats received thermal injury on the back according to the previously described "comb burn" model. Thirty minutes after the burn injury, mesenchymal stem cells were injected subcutaneously to the stasis zone of the experimental group (n = 10). Animals in the control group (n = 10) were given the same amount of saline without mesenchymal stem cells. Animals in the sham group (n = 6) did not receive any thermal trauma. Seventy-two hours after the burn injury, scintigraphic examination was applied to determine average vital tissue at the stasis zone. Thereafter, skin samples were assessed by immunohistochemistry assay for apoptosis count. The blood samples drawn before and 72 hours after the burn injury were analyzed to determine systemic cytokine levels.
  
  
  RESULTS
  The apoptosis count of the control group was found to be significantly higher than that of the experimental group. Vital tissue percentage of the stasis zone was significantly higher for the experimental group than for the control group. The cytokine levels did not reveal any statistically significant difference between the groups.
  
  
  CONCLUSIONS
  Apoptosis count and scintigraphic results of this study confirm that mesenchymal stem cell treatment has a statistically significant benefit for the survival of the stasis zone in acute burn.
authors: Istanbul, Turkey From the Departments of Plastic Reconstructive and Aesthetic Surgery and Burn Unit, Infectious Disease and Clinical Microbiology, Pathology, and Nuclear Medicine, Gulhane Military Medical Academy, Haydarpasa Training Hospital, and the Department of Genetics and Bioengineering, Yeditepe University.
authors: Sinan
authors: S
authors: Öksüz
authors: Ersin
authors: E
authors: Ülkür
authors: Oral
authors: O
authors: Öncül
authors: Gamze Torun
authors: GT
authors: Köse
authors: Zafer
authors: Z
authors: Küçükodac
authors: Muammer
authors: M
authors: Urhan
mesh_terms: D000818:Animals; D017209:Apoptosis; D002056:Burns / Q000473:pathology* / Q000601:surgery*; D004195:Disease Models, Animal; D007279:Injections, Subcutaneous; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley