title: COG complexes form spatial landmarks for distinct SNARE complexes.
abstract: Vesicular tethers and SNAREs (soluble N-ethylmalemide-sensitive fusion attachment protein receptors) are two key protein components of the intracellular membrane-trafficking machinery. The conserved oligomeric Golgi (COG) complex has been implicated in the tethering of retrograde intra-Golgi vesicles. Here, using yeast two-hybrid and co-immunoprecipitation approaches, we show that three COG subunits, namely COG4, 6 and 8, are capable of interacting with defined Golgi SNAREs, namely STX5, STX6, STX16, GS27 and SNAP29. Comparative analysis of COG8-STX16 and COG4-STX5 interactions by a COG-based mitochondrial relocalization assay reveals that the COG8 and COG4 proteins initiate the formation of two different tethering platforms that can facilitate the redirection of two populations of Golgi transport intermediates to the mitochondrial vicinity. Our results uncover a role for COG sub-complexes in defining the specificity of vesicular sorting within the Golgi.
authors: Department of Physiology and Biophysics, UAMS, Little Rock, Arkansas 72205, USA.
authors: Rose
authors: R
authors: Willett
authors: Tetyana
authors: T
authors: Kudlyk
authors: Irina
authors: I
authors: Pokrovskaya
authors: Robert
authors: R
authors: Schönherr
authors: Daniel
authors: D
authors: Ungar
authors: Rainer
authors: R
authors: Duden
authors: Vladimir
authors: V
authors: Lupashin
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002522:Chlorocebus aethiops; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D050600:SNARE Proteins / Q000737:chemistry / Q000378:metabolism*; D022621:Shiga Toxin / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism / Q000648:ultrastructure; D014709:Vero Cells
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D015415:Biomarkers; D046912:Multiprotein Complexes; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; D049452:Green Fluorescent Proteins; D022621:Shiga Toxin