title: The identification of apolipoprotein C-I in rare minnow (Gobiocypris rarus) and its expression following cadmium exposure.
abstract: Cadmium (Cd) is a ubiquitous environment contaminant that has been detected in a variety of terrestrial and aquatic organisms. In our study, rare minnow were exposed to 0.4 mg/L Cd(2+) solution, fish tissues including gill, intestine, kidney, liver, muscle, and spleen were collected at 1, 6, 12, 24 and 48 h after exposure. Differential expression sequence tags (ESTs) were screened and an EST similar to Hemibarbus mylodon apolipoprotein C-I (ApoC-I) was identified. The ApoC-I cDNA had been obtained with the sequence of 583 bp, which can code 84 amino acids. The transcription expression patterns of ApoC-I were compared after Cd exposure with the real-time PCR in the above tissues. ApoC-I regulation was prominent in spleen, muscle, gill and liver and moderate in kidney and intestine. This suggests a complex transcriptional regulation of ApoC-I expression following Cd exposure.
authors: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province 430072, PR China.
authors: Chunling
authors: C
authors: Wang
authors: Futie
authors: F
authors: Zhang
authors: Wenxuan
authors: W
authors: Cao
authors: Jianwei
authors: J
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053302:Apolipoprotein C-I / Q000235:genetics*; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D003530:Cyprinidae; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D029941:Fish Proteins / Q000235:genetics*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D053302:Apolipoprotein C-I; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D002104:Cadmium