title: Immunophenotypic profile of nucleated erythroid progenitors during maturation in regenerating bone marrow.
abstract: This study introduces an in-depth flow cytometric method for the analysis of nucleated erythroid progenitors during bone marrow regeneration. Initial immunophenotypic analysis with the conventional erythroid-associated markers CD36, CD71 and CD235a was supplemented with the analysis of additional markers, including CD105, CD117, CD45, CD38 and cell-scattered light characteristics. Our data show that the expression of CD105 and CD117 is critical for the distinction between four phenotypically different developmental stages of nucleated erythroid progenitors: pro-erythroblasts, basophilic erythroblasts, polychromatophilic erythroblasts and orthochromatophilic erythroblasts. CD105 antigen expression was specifically associated with pro-erythroblasts and basophilic erythroblasts, whereas CD117 was expressed at the earliest pro-erythroblast stage. Both antigens were progressively lost throughout the course of differentiation. These data allow for the identification of aberrant erythroid development in acute erythroid leukemia or myelodysplastic syndrome.
authors: Cancer Research Institute.
authors: Michaela
authors: M
authors: Fajtova
authors: Anna
authors: A
authors: Kovarikova
authors: Peter
authors: P
authors: Svec
authors: Esko
authors: E
authors: Kankuri
authors: Jan
authors: J
authors: Sedlak
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D002454:Cell Differentiation; D004912:Erythrocytes / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism
chemical_list: D015703:Antigens, CD