title: Cytokine response to acute running in recreationally-active and endurance-trained men.
abstract: To compare the cytokine response to exhaustive running in recreationally-active (RA) and endurance-trained (ET) men. Eleven RA men (VO2max 55 ± 7 mL·min(-1)·kg(-1)) and 10 ET men (VO₂max 68 ± 7 mL·min(-1)·kg(-1)) followed a controlled diet and refrained from volitional exercise for 8 days. On the fourth day, participants completed 60 min of treadmill running (65 % VO₂max), followed by intermittent running to exhaustion (70 % VO₂max). Fasting blood was obtained at baseline, after 20, 40 and 60 min of exercise, at the end of intermittent exercise, during 2 h of recovery and on four follow-up days (FU1-FU4). Tumour necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-1 receptor antagonist (IL-1ra) and creatine kinase (CK) were measured. Exercise increased the concentrations of all cytokines and CK, but there were no significant differences between groups. IL-1β increased (2.2-2.5-fold, P < 0.001) during exercise, while TNF-α was increased (1.6-2.0-fold, P < 0.001) during exercise and for 2 h post-exercise. IL-6 (71-84-fold, P < 0.001) and IL-1ra (52-64-fold, P < 0.001) were increased throughout exercise and up to FU1, peaking immediately after exercise and at 1.5-2 h post-exercise, respectively. CK concentrations were increased (P < 0.001) throughout exercise and up to FU4, peaking at FU1, but were not associated with changes in any cytokines. Exhaustive running resulted in modest and transient increases in TNF-α and IL-1β, and more marked and prolonged increases in IL-6 and IL-1ra, but improved training status did not affect this response. Increased CK might indicate either exercise-induced muscle cell disruption or increased cell permeability, although neither appears to have contributed to the increased cytokine concentrations.
authors: Human Sciences, QinetiQ Ltd, Room 2022, Building A5, QinetiQ Ltd, Cody Technology Park, Ively Road, GU14 0LX, Farnborough, Hampshire, UK. jscott1@qinetiq.com
authors: Jonathan P R
authors: JP
authors: Scott
authors: Craig
authors: C
authors: Sale
authors: Julie P
authors: JP
authors: Greeves
authors: Anna
authors: A
authors: Casey
authors: John
authors: J
authors: Dutton
authors: William D
authors: WD
authors: Fraser
mesh_terms: D000328:Adult; D015308:Anaerobic Threshold; D003402:Creatine Kinase / Q000097:blood; D016207:Cytokines / Q000097:blood*; D006801:Humans; D008297:Male; D012420:Running / Q000502:physiology*
chemical_list: D016207:Cytokines; D003402:Creatine Kinase