title: [Effect of 4B3 monoclonal antibody on the experimental Cryptococcus neoformans infection].
abstract: BACKGROUND
  frequent relapses and high lethality of criptococcosis has encouraged the search for new therapeutic strategies.
  
  
  OBJECTIVE
  to evaluate the effect of the monoclonal antibody 4B3 on the cryptococcal infection in Balb/c mice.
  
  
  METHODS
  the kinetics in serum concentration of the studied monoclonal antibody was determined for intraperitoneal administration (500 microg) by quantitative sandwich ELISA. In order to assess its protective capability, were administered 500 microg of 4B3 and innoculated 2 x 10(22) cells/mL of Cryptococcus neoformans. The survival of mice was recorded and the yeast dissemination to the main target organs was evaluated. Macrophages P338.D1 cell lines were used to measure the effect of the monoclonal antibody 4B3 on the phagocytosis and lysis of the microorganism.
  
  
  RESULTS
  the used dose helped to keep high values (38 microg/m) of 4B3 in serum for at least 46 days. It was found that the monoclonal antibody does not give protection, which makes the microorganism dissemination possible, along with the reduction in the survival of mice. Finally, the phagocytosis test showed that 4B3 increased the phagocytic activity of macrophages without any fungicidal effect.
  
  
  CONCLUSIONS
  the monoclonal antibody 4B3 stimulates C. neoformans phagocytosis by macrophages without fungicidal effect, thus favoring yeast dissemination and decreasing the survival of mice due to cryptococcal infection
authors: Instituto de Medicina Tropical "Pedro Kourí", La Habana, Cuba. mtilnait@ipk.sld.cu
authors: María Teresa
authors: MT
authors: Illnait-Zaragozí
authors: René
authors: R
authors: Gato Armas
authors: Gerardo Félix
authors: GF
authors: Martínez Machín
authors: Anselmo
authors: A
authors: Otero González
authors: Jorge
authors: J
authors: Sarracent Pérez
authors: Hermis
authors: H
authors: Rodríguez Sánchez
authors: Carlos Manuel
authors: CM
authors: Fernández Andreu
authors: Iliana del Carmen
authors: Idel C
authors: Valdés Hernández
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D003453:Cryptococcosis; D003455:Cryptococcus neoformans / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal