title: MCP-1, CCR2 and CCR5 polymorphisms in Tunisian patients with atopic asthma.
abstract: Chemokines and their receptors play an important role in the late inflammatory stage of asthma. In this study, we aimed to investigate polymorphisms of MCP-1 (CCL2), CCR2 and CCR5 which can affect qualitatively and/or quantitatively their production and thus influence both susceptibility and severity of asthma and its clinical and biological features.MCP-1 (A/G -2518), CCR2 (+/64I), CCR5 (G/A -59029) and CCR5 (∆32) polymorphisms were evaluated by PCR in 107 Tunisian patients with asthma and 169 healthy controls.No significant association was found between the four investigated polymorphisms and asthma. Nevertheless the haplotype MCP1*AG/CCR2*+/+ was significantly l ess frequent in patients (20.5%) compared to controls (32.5%) (p=0.03; OR=0.54; 95% CI: 0.29-0.98). Whereas no difference was observed in CCR2/CCR5 haplotypes between patients and controls. Analysis of polymorphisms with clinical and biological features showed that the concomitant presence of MCP-1*G/CCR2*64I alleles was less frequent in severe forms (4.34%) compared to moderate disease (12%) but the difference was not significant (p=0.27). No association was observed between the four polymorphisms and the presence of atopic rhinitis or atopic conjunctivitis and an elevated rate of serum IgE over 200 IU/ml.Additional effects of MCP-1 and its receptor CCR2 polymorphisms seem to be involved in disease susceptibility to asthma in Tunisian patients; nevertheless they could be protective against its severe forms.
authors: Laboratory of Research in Immunology of Renal Transplantation and Immunopathology (LR03SP01), Charles Nicolle Hospital, Tunis, El Manar University, Bd 9 Avril, 1006 Bab Saadoun, Tunis, Tunisia. gorgi.yousr@gmail.com.
authors: Tarak
authors: T
authors: Dhaouadi
authors: Imen
authors: I
authors: Sfar
authors: Hajer
authors: H
authors: Aounallah-Skhiri
authors: Saloua
authors: S
authors: Jendoubi-Ayed
authors: Hend
authors: H
authors: Bouacha
authors: Taieb
authors: T
authors: Ben Abdallah
authors: Yousr
authors: Y
authors: Gorgi
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics* / Q000276:immunology; D005260:Female; D060005:Genotyping Techniques; D006239:Haplotypes; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D054390:Receptors, CCR2 / Q000235:genetics* / Q000276:immunology; D019713:Receptors, CCR5 / Q000235:genetics* / Q000276:immunology; D012720:Severity of Illness Index; D014416:Tunisia
chemical_list: C111835:CCL2 protein, human; C515334:CCR2 protein, human; D018932:Chemokine CCL2; D054390:Receptors, CCR2; D019713:Receptors, CCR5