title: Comparison of HPV DNA testing in cervical exfoliated cells and tissue biopsies among HIV-positive women in Kenya.
abstract: HIV-positive women are infected with human papillomavirus (HPV) (especially with multiple types), and develop cervical intraepithelial neoplasia (CIN) and cervical cancer more frequently than HIV-negative women. We compared HPV DNA prevalence obtained using a GP5+/6+ PCR assay in cervical exfoliated cells to that in biopsies among 468 HIV-positive women from Nairobi, Kenya. HPV prevalence was higher in cells than biopsies and the difference was greatest in 94 women with a combination normal cytology/normal biopsy (prevalence ratio, PR = 3.7; 95% confidence interval, CI: 2.4-5.7). PR diminished with the increase in lesion severity (PR in 58 women with high-grade squamous intraepithelial lesions (HSIL)/CIN2-3 = 1.1; 95% CI: 1.0-1.2). When HPV-positive, cells contained 2.0- to 4.6-fold more multiple infections than biopsies. Complete or partial agreement between cells and biopsies in the detection of individual HPV types was found in 91% of double HPV-positive pairs. The attribution of CIN2/3 to HPV16 and/or 18 would decrease from 37.6%, when the presence of these types in either cells or biopsies was counted, to 20.2% when it was based on the presence of HPV16 and/or 18 (and no other types) in biopsies. In conclusion, testing HPV on biopsies instead of cells results in decreased detection but not elimination of multiple infections in HIV-positive women. The proportion of CIN2/3 attributable to HPV16 and/or 18 among HIV-positive women, which already appeared to be lower than that in HIV-negative, would then further decrease. The meaning of HPV detection in cells and random biopsy from HIV-positive women with no cervical abnormalities remains unclear.
authors: International Agency for Research on Cancer, Lyon, France. devuysth@iarc.fr
authors: Hugo
authors: H
authors: De Vuyst
authors: Michael H
authors: MH
authors: Chung
authors: Iacopo
authors: I
authors: Baussano
authors: Nelly R
authors: NR
authors: Mugo
authors: Vanessa
authors: V
authors: Tenet
authors: Folkert J
authors: FJ
authors: van Kemenade
authors: Farzana S
authors: FS
authors: Rana
authors: Samah R
authors: SR
authors: Sakr
authors: Chris J L M
authors: CJ
authors: Meijer
authors: Peter J F
authors: PJ
authors: Snijders
authors: Silvia
authors: S
authors: Franceschi
mesh_terms: D000328:Adult; D001706:Biopsy; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D061346:Laser Capture Microdissection; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Africa; HIV; cervical exfoliated cells; cervical neoplasia; cervical tissue biopsy; human papillomavirus