title: Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics.
abstract: We present a novel "Lab-on-DVD" system and demonstrate its capability for rapid and low-cost HIV diagnostics by counting CD4+ cells isolated from whole blood. We show that a commercial DVD drive can, with certain modifications, be turned into an improved DVD-based laser scanning microscope (DVD-LSM). The system consists of a multi-layered disposable polymer disc and a modified commercial DVD reader with rotational control for sample handling, temperature control for optimized bioassay, a photodiode array for detection, and software for signal processing and user interface - all the necessary components required for a truly integrated lab-on-a-chip system, with the capability to deliver high-resolution images down to 1 μm in size. Using discs modified with antibodies, we specifically captured CD4+ cells from whole blood, demonstrating single cell resolution imaging. The novel integrated DVD platform with sub-micron image resolution brings, for the first time, affordable cellular diagnostic testing to the point-of-care and should be readily applicable at resource-limited settings.
authors: Science for Life Laboratory, Div. of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, Stockholm, Sweden.
authors: Harisha
authors: H
authors: Ramachandraiah
authors: Mary
authors: M
authors: Amasia
authors: Jackie
authors: J
authors: Cole
authors: Paul
authors: P
authors: Sheard
authors: Simon
authors: S
authors: Pickhaver
authors: Chris
authors: C
authors: Walker
authors: Valtteri
authors: V
authors: Wirta
authors: Preben
authors: P
authors: Lexow
authors: Richard
authors: R
authors: Lione
authors: Aman
authors: A
authors: Russom
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D004581:Electronics; D005434:Flow Cytometry; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal; D019095:Point-of-Care Systems; D012984:Software; D013696:Temperature
chemical_list: D055910:Antibodies, Immobilized