title: A detailed study of gold-nanoparticle loaded cells using X-ray based techniques for cell-tracking applications with single-cell sensitivity.
abstract: In the present study complementary high-resolution imaging techniques on different length scales are applied to elucidate a cellular loading protocol of gold nanoparticles and subsequently its impact on long term and high-resolution cell-tracking utilizing X-ray technology. Although demonstrated for malignant cell lines the results can be applied to non-malignant cell lines as well. In particular the accumulation of the gold marker per cell has been assessed quantitatively by virtue of electron microscopy, two-dimensional X-ray fluorescence imaging techniques and X-ray CT with micrometric and sub-micrometric resolution. Moreover, utilizing these techniques the three dimensional distribution of the incorporated nanoparticles, which are sequestered in lysosomes as a permanent marker, could be determined. The latter allowed elucidation of the gold partition during mitosis and the cell size, which subsequently enabled us to define the optimal instrument settings of a compact microCT system to visualize gold loaded cells. The results obtained demonstrate the feasibility of cell-tracking using X-ray CT with compact sources.
authors: Australian Synchrotron Company Ltd, 800, Blackburn Rd., Clayton, VIC 3168, Australia. alberto.asto@gmail.com
authors: Alberto
authors: A
authors: Astolfo
authors: Fulvia
authors: F
authors: Arfelli
authors: Elisabeth
authors: E
authors: Schültke
authors: Simon
authors: S
authors: James
authors: Lucia
authors: L
authors: Mancini
authors: Ralf-Hendrik
authors: RH
authors: Menk
mesh_terms: D000818:Animals; D048429:Cell Size; D058948:Cell Tracking / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D006046:Gold / Q000737:chemistry*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008938:Mitosis; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D059010:Single-Cell Analysis / Q000379:methods*; D014183:Transplantation, Heterologous; D055114:X-Ray Microtomography; D014965:X-Rays
chemical_list: D006046:Gold