title: Effect of You Gui Wan on mouse sperm fertilising ability in vivo and in vitro.
abstract: This study is to explore whether YGW has an impact on sperm fertilising ability in mice. Twenty male mice were randomly divided into two groups. In vivo experiments, one group of animals were orally administrated with YGW decoction and another group administered with saline for 14 days. Afterwards, the animals were mated with their female partners. Percentages of retrieved zygotes were then compared. In vitro experiments, in vitro fertilisation (IVF) assay, sperm acrosome reaction and acrosin activity were used to compare sperm fertilising ability between the two groups. The YGW-treated group had a significantly higher percentage of zygotes than the saline controls (P = 0.005). The IVF rates induced by spermatozoa from the herb-treated mice were also significantly higher than those from the control animals (P = 0.015). The sperm acrosin activity of the herb-treated group was significantly higher than that of the saline-treated group (P = 0.048), although there was no significant difference in testicular weight, sperm count and sperm motility. These data suggest that YGW decoction has a significant effect on normal sperm fertilising ability both in vivo and in vitro, which may be due to, at least in part, increments in the sperm acrosin activity.
authors: Department of Gynecology and Obstetrics, The Second Medical College/Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
authors: X-H
authors: XH
authors: Jiang
authors: S-M
authors: SM
authors: Yie
authors: X
authors: X
authors: Zhen
authors: Y-L
authors: YL
authors: Den
authors: X
authors: X
authors: Liang
authors: X
authors: X
authors: Hu
authors: L-M
authors: LM
authors: Li
authors: Q-J
authors: QJ
authors: Li
authors: S
authors: S
authors: Cao
authors: H
authors: H
authors: Lu
mesh_terms: D000176:Acrosin / Q000378:metabolism; D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005306:Fertilization / Q000187:drug effects* / Q000502:physiology; D005307:Fertilization in Vitro; D007248:Infertility, Male / Q000188:drug therapy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; C502768:you-gui-wan; D000176:Acrosin
keywords: Acrosin activity; You Gui Wan; acrosome reaction; sperm fertilising ability