title: Differentially regulated microRNAs during human sebaceous lipogenesis.
abstract: BACKGROUND
  MicroRNAs (miRNAs) regulate several aspects of the morphogenesis and homeostasis of the skin and its appendages, and miRNA deregulation has been shown to be associated or even causally related to several skin diseases.
  
  
  OBJECTIVE
  To evaluate the differential expression of miRNAs during sebaceous lipogenesis.
  
  
  METHODS
  Inhibition of global miRNA activity in human SZ95 sebaceous gland cells was achieved by transfection with siRNAs directed against the DICER transcript, encoding a key enzyme of miRNA biogenesis. Sebaceous lipogenesis was induced in SZ95 sebocytes by addition of linoleic acid (LA) and ciglitazone (CIG) and microarray-based miRNA expression profiles were obtained on an Agilent platform. The expression of selected miRNA candidates was measured by Taqman quantitative real-time RT-PCR. Increased activity of one validated miRNA was attained by transfecting SZ95 sebocytes with miR-574-3p mimics.
  
  
  RESULTS
  Downregulation of sebaceous lipogenesis was detected in DICER-impaired SZ95 sebocytes. Using microarrays, we identified twelve significantly upregulated and nine significantly downregulated miRNAs in LA- and CIG-treated SZ95 sebocytes as compared to non-treated cells. Validation of a subset of miRNA candidates by qRT-PCR confirmed upregulation of mIR-203 and miR-574-3p and downregulation of miR-7 during sebaceous lipogenesis. The two upregulated miRNAs have been previously implied in keratinocyte differentiation. Increased activity of miR-574-3p augmented lipogenesis in SZ95 sebocytes.
  
  
  CONCLUSIONS
  Global miRNA activity is essential for lipid synthesis in human SZ95 sebocytes. Individual miRNAs are likely to play a significant role during sebaceous lipogenesis.
authors: Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Munich, Germany. schnder@lmb.uni-muenchen.de
authors: Marlon R
authors: MR
authors: Schneider
authors: Anastazia
authors: A
authors: Samborski
authors: Stefan
authors: S
authors: Bauersachs
authors: Christos C
authors: CC
authors: Zouboulis
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D019787:Linoleic Acid / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology; D050155:Lipogenesis / Q000187:drug effects / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D043244:Ribonuclease III / Q000235:genetics / Q000502:physiology; D012627:Sebaceous Glands / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: C546703:MIRN203 microRNA, human; C558811:MIRN574 microRNA, human; D035683:MicroRNAs; D045162:Thiazolidinediones; D019787:Linoleic Acid; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases; C039671:ciglitazone