title: Effects of coenzyme Q(10) on LDL oxidation in vitro.
abstract: Coenzyme Q(10), a lipid soluble benzoquinone, has excellent antioxidant and membrane stabilizing properties in the cardiac tissue. The present study quantitatively investigated the effects of coenzyme Q(10) on in vitro LDL oxidation. The formation of conjugated dienes, malondialdehyde (MDA) and electrophoretic mobility were monitored as markers of the oxidation of LDL, respectively. Coenzyme Q(10) showed a decrease the formation of conjugated dienes and MDA, respectively, against oxidation in vitro. Coenzyme Q(10) also reduced electrophoretic mobility of the oxidized human LDL. Thus, results showed that the coenzyme Q(10) exhibits strong antioxidant activity in CuSO(4)-mediated oxidation of LDL (P<0.05) in vitro. The inhibitory effects of the coenzyme Q(10) on LDL oxidation were dose-dependent at concentrations ranging from 20 nM to 300 nM. Moreover, the effects of coenzyme Q(10) on LDL oxidation were compared with vitamin E and vitamin C. This study showed that coenzyme Q(10) is a potent antioxidant to protect LDL against oxidation in vitro and may be a good alternative to reduce the risk of atherosclerosis and coronary heart disease and other free radical associated health problems.
authors: Department of Biochemistry, Lorestan University of Medical Sciences, Khoramabad, Iran. hassan_a46@yahoo.com
authors: Hassan
authors: H
authors: Ahmadvand
authors: Hiroshi
authors: H
authors: Mabuchi
authors: Atsushi
authors: A
authors: Nohara
authors: Junji
authors: J
authors: Kobayahi
authors: Masa-aki
authors: MA
authors: Kawashiri
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D019327:Copper Sulfate / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D014451:Ubiquinone / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D014451:Ubiquinone; D014810:Vitamin E; D008315:Malondialdehyde; D019327:Copper Sulfate; D001205:Ascorbic Acid