title: Matrix Gla protein is involved in crystal formation in kidney of hyperoxaluric rats.
abstract: BACKGROUND
  Matrix Gla protein (MGP) is a molecular determinant regulating vascular calcification of the extracellular matrix. However, it is still unclear how MGP may be involved in crystal formation in the kidney of hyperoxaluric rats.
  
  
  METHODS
  Male Sprague-Dawley rats were divided into the hyperoxaluric group and control group. Hyperoxaluric rats were administrated by 0.75% ethylene glycol (EG) for up to 8 weeks. Renal MGP expression was detected by the standard avidin-biotin complex (ABC) method. Renal crystal deposition was observed by a polarizing microscope. Total RNA and protein from the rat kidney tissue were extracted. The levels of MGP mRNA and protein expression were analyzed by the real-time polymerase chain reaction (RT-PCR) and Western blot.
  
  
  RESULTS
  Hyperoxaluria was induced successfully in rats. The MGP was polarly distributed, on the apical membrane of renal tubular epithelial cells, and was found in the ascending thick limbs of Henle's loop (cTAL) and the distal convoluted tubule (DCT) in hyperoxaluric rats, its expression however, was present in the medullary collecting duct (MCD) in stone-forming rats. Crystals with multilaminated structure formed in the injurious renal tubules with lack of MGP expression.MGP mRNA expression was significantly upregulated by the crystals' stimulations.
  
  
  CONCLUSIONS
  Our results suggested that the MGP was involved in crystals formation by the continuous expression, distributing it polarly in the renal tubular cells and binding directly to the crystals.
authors: School of Life Sciences, Liaoning University, Shenyang 110036, China.
authors: Xiuli
authors: X
authors: Lu
authors: Bing
authors: B
authors: Gao
authors: Takahiro
authors: T
authors: Yasui
authors: Yang
authors: Y
authors: Li
authors: Ting
authors: T
authors: Liu
authors: Xu
authors: X
authors: Mao
authors: Masahito
authors: M
authors: Hirose
authors: Yuhong
authors: Y
authors: Wu
authors: Daojun
authors: D
authors: Yu
authors: Qiwen
authors: Q
authors: Zhu
authors: Kenjiro
authors: K
authors: Kohri
authors: Chunling
authors: C
authors: Xiao
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D019855:Ethylene Glycol / Q000633:toxicity; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D006959:Hyperoxaluria / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007669:Kidney Calculi / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000097568:Matrix Gla Protein
chemical_list: D002135:Calcium-Binding Proteins; D019855:Ethylene Glycol; D016326:Extracellular Matrix Proteins; D000097568:Matrix Gla Protein