title: Wine extracts from Sardinian grape varieties attenuate membrane oxidative damage in Caco-2 cell monolayers.
abstract: One of the most important sites of polyphenol action seems to be in the gastrointestinal system before absorption. We investigated the ability of three wine phenolic extracts, obtained from grape varieties grown in Sardinia, Cannonau (red), Vermentino and Malvasia (white), to exert an antioxidant action against tert-butyl hydroperoxide (TBH)-induced oxidative damage to Caco-2 cell monolayers as a model system of the human intestine. TBH treatment caused the disruption of epithelial integrity, measured as transepithelial electrical resistance, and markers of the peroxidation process of membrane lipids, MDA, fatty acid hydroperoxides and 7-ketocholesterol. All wine extracts were able to counteract the oxidising action of TBH and, in spite of the differences in phenolic composition, exerted a comparable activity. Our findings point out a direct antioxidant action of the wine extracts on enterocytes exposed to oxidising species and further support the opinion that total phenolic content is not essential for antioxidant activity.
authors: Dipartimento di Biologia Sperimentale, Sezione di Patologia Sperimentale, Università degli Studi di Cagliari, Cittadella Universitaria SS 554, 09042 Monserrato, Cagliari, Italy. mdeiana@unica.it
authors: Monica
authors: M
authors: Deiana
authors: Debora
authors: D
authors: Loru
authors: Alessandra
authors: A
authors: Incani
authors: Antonella
authors: A
authors: Rosa
authors: Angela
authors: A
authors: Atzeri
authors: Maria Paola
authors: MP
authors: Melis
authors: Barbara
authors: B
authors: Cabboi
authors: Laurent
authors: L
authors: Hollecker
authors: Maria Barbara
authors: MB
authors: Pinna
authors: Francesca
authors: F
authors: Argiolas
authors: Mariano
authors: M
authors: Murru
authors: Maria Assunta
authors: MA
authors: Dessì
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D018938:Caco-2 Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols