title: Oxidative stress mediated-alterations of the microRNA expression profile in mouse hippocampal neurons.
abstract: Oxidative stress plays a critical role in the etiology and pathogenesis of neurodegenerative disorders, and the molecular mechanisms that control the neuron response to ROS have been extensively studied. However, the oxidative stress-effect on miRNA expression in hippocampal neurons has not been investigated, and little is known on the effect of ROS-modulated miRNAs on cell function. In this study, H(2)O(2) was used to stimulate the mouse primary hippocampal neurons to develop an oxidative stress cell model. The alterations of miRNAs expression were detected by microarray analysis and five miRNAs were validated by real-time RT-PCR. The bioinformatic analysis of deregulated miRNAs was performed to determine their potential roles in the pathogenesis of neurological disorders. We found that H(2)O(2) mediated a total of 101 deregulated miRNAs, which mainly took part in the regulation of the MAPK pathway. Among them, miR-135b and miR-708 were up-regulated significantly and their targets were predicted to be involved in DNA recombination, protein ubiquitination, protein autophosphorylation and development of neurons. These results demonstrated that oxidative stress alters the miRNA expression profile of hippocampal neurons, and the deregulated miRNAs might play a potential role in the pathogenesis of neurodegenerative diseases, such as Alzheimer's disease (AD).
authors: Central Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China. zhangqfdoctor@163.com.
authors: Shunjiang
authors: S
authors: Xu
authors: Rui
authors: R
authors: Zhang
authors: Jingya
authors: J
authors: Niu
authors: Dongsheng
authors: D
authors: Cui
authors: Bing
authors: B
authors: Xie
authors: Binggui
authors: B
authors: Zhang
authors: Kang
authors: K
authors: Lu
authors: Wenjun
authors: W
authors: Yu
authors: Xueyi
authors: X
authors: Wang
authors: Qingfu
authors: Q
authors: Zhang
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis*; D019636:Neurodegenerative Diseases / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D016877:Oxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide