title: Staphylococcus aureus phenol-soluble modulin peptides modulate dendritic cell functions and increase in vitro priming of regulatory T cells.
abstract: The major human pathogen Staphylococcus aureus has very efficient strategies to subvert the human immune system. Virulence of the emerging community-associated methicillin-resistant S. aureus depends on phenol-soluble modulin (PSM) peptide toxins, which are known to attract and lyse neutrophils. However, their influences on other immune cells remain elusive. In this study, we analyzed the impact of PSMs on dendritic cells (DCs) playing an essential role in linking innate and adaptive immunity. In human neutrophils, PSMs exert their function by binding to the formyl peptide receptor (FPR) 2. We show that mouse DCs express the FPR2 homolog mFPR2 as well as its paralog mFPR1 and that PSMs are chemoattractants for DCs at noncytotoxic concentrations. PSMs reduced clathrin-mediated endocytosis and inhibited TLR2 ligand-induced secretion of the proinflammatory cytokines TNF, IL-12, and IL-6, while inducing IL-10 secretion by DCs. As a consequence, treatment with PSMs impaired the capacity of DCs to induce activation and proliferation of CD4(+) T cells, characterized by reduced Th1 but increased frequency of FOXP3(+) regulatory T cells. These regulatory T cells secreted high amounts of IL-10, and their suppression capacity was dependent on IL-10 and TGF-β. Interestingly, the induction of tolerogenic DCs by PSMs appeared to be independent of mFPRs, as shown by experiments with mice lacking mFPR2 (mFPR2(-/-)) and the cognate G protein (p110γ(-/-)). Thus, PSMs from highly virulent pathogens affect DC functions, thereby modulating the adaptive immune response and probably increasing the tolerance toward the pathogen.
authors: Interfaculty Institute for Cell Biology, Eberhard Karls University, 72076 Tübingen, Germany.
authors: Jens
authors: J
authors: Schreiner
authors: Dorothee
authors: D
authors: Kretschmer
authors: Juliane
authors: J
authors: Klenk
authors: Michael
authors: M
authors: Otto
authors: Hans-Jörg
authors: HJ
authors: Bühring
authors: Stefan
authors: S
authors: Stevanovic
authors: Ji Ming
authors: JM
authors: Wang
authors: Sandra
authors: S
authors: Beer-Hammer
authors: Andreas
authors: A
authors: Peschel
authors: Stella E
authors: SE
authors: Autenrieth
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000276:immunology*; D002633:Chemotaxis / Q000276:immunology; D002966:Clathrin / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004705:Endocytosis / Q000276:immunology; D005260:Female; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D010455:Peptides / Q000276:immunology*; D044042:Receptors, Formyl Peptide / Q000378:metabolism; D013211:Staphylococcus aureus / Q000737:chemistry / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D002966:Clathrin; D016207:Cytokines; D010455:Peptides; D044042:Receptors, Formyl Peptide; D051195:Toll-Like Receptor 2; C025971:staphylococcal delta toxin