title: Herbaspirillum psychrotolerans sp. nov., a member of the family Oxalobacteraceae from a glacier forefield.
abstract: A novel psychrotolerant, Gram-negative, shiny white, curved-rod-shaped, facultatively anaerobic bacterium PB1(T) was isolated from a soil sample collected from a glacier forefield of the Larsemann Hills, East Antarctica. Isolate PB1(T) has catalase and low urease activity and hydrolyses gelatin and starch. Strain PB1(T) is able to grow between -5 °C and 30 °C with optimum growth at 14-20 °C. Glycerol, dl-arabinose, d-xylose, d-galactose, d-fructose, d-lyxose, d-fucose and potassium gluconate are used as sole carbon sources. The major quinone is ubiquinone Q-8. The major fatty acids (>10%) for PB1(T) are C(16:0) (19.1%), C(16:1)ω7cis (44.6%) and C(18:1)ω7cis (16.2%). The major polyamines are putrescine [54.9 µmol (g dry weight)(-1)] and 2-hydroxy putrescine [18.5 µmol (g dry weight)(-1)]. DNA G+C content is 62.5 mol%. Strain PB1(T) is phylogenetically related to species of the genus Herbaspirillum, with highest 16S rRNA gene sequence similarities to Herbaspirillum canariense (97.3%), Herbaspirillum aurantiacum (97.2%), Herbaspirillum soli (97.2%) and Herbaspirillum frisingense (97.0%). The DNA-DNA relatedness values were below 30% between PB1(T) and the type strains of Herbaspirillum canariense, Herbaspirillum aurantiacum and Herbaspirillum soli. The different geographical origin of strain PB1(T) from its closest phylogenetic relatives resulted in different phenotypic and genotypic specifications, whereby strain PB(T) represents a novel species of the genus Herbaspirillum, for which the name Herbaspirillum psychrotolerans is proposed. The type strain is PB1(T) (DSM 26001(T) =LMG 27282(T)).
authors: Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Germany.
authors: Felizitas
authors: F
authors: Bajerski
authors: University of Tromsø, Department for Arctic and Marine Biology, Tromsø, Norway.
authors: Lars
authors: L
authors: Ganzert
authors: Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Section 4.3, Organic Geochemistry, Germany.
authors: Kai
authors: K
authors: Mangelsdorf
authors: Rheinische Friedrich-Wilhelms-Universität Bonn, Institute of Nutrition and Food Sciences, Department of Food Microbiology and Hygiene, Bonn, Germany.
authors: André
authors: A
authors: Lipski
authors: University of Veterinary Medicine Vienna, Division for Clinical Microbiology and Infectious Biology, Vienna, Austria.
authors: Hans-Jürgen
authors: HJ
authors: Busse
authors: Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Germany.
authors: Lisa
authors: L
authors: Padur
authors: Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Section 4.5 Geomicrobiology, Potsdam, Germany.|Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Germany.
authors: Dirk
authors: D
authors: Wagner
mesh_terms: D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D042803:Herbaspirillum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8