title: Rat bone marrow stem cells isolation and culture as a bone formative experimental system.
abstract: Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe pioneering experiment of tissue engineering in Bosnia and Herzegovina, of the isolation and differentiation rat bone marrow stromal cells in to the osteoblast cells lineages. Rat bone marrow stromal cells were isolated by method described by Maniatopulos using their plastic adherence capatibility. The cells obtained by plastic adherence were cultured and serially passaged in the osteoinductive medium to differentiate into the osteocytes. Bone marrow samples from rats long bones used for isolation of stromal cells (BMSCs). Under determinate culture conditions BMSCs were differentiated in osteogenic cell lines detected by Alizarin red staining three weeks after isolation. BMSCs as autologue cells model showed high osteogenetic potential and calcification capatibility in vitro. In future should be used as alternative method for bone transplantation in Regenerative Medicine.
authors: Clinic for Maxillofacial Surgery, Clinical University Center, H. Sabanovica 1, 71000 Sarajevo, Bosnia and Herzegovina. amersmajilagic@hotmail.com
authors: Amer
authors: A
authors: Smajilagić
authors: Mufida
authors: M
authors: Aljičević
authors: Amira
authors: A
authors: Redžić
authors: Selma
authors: S
authors: Filipović
authors: Alena
authors: A
authors: Lagumdžija
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology; D001861:Bone Regeneration; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology*; D051381:Rats
chemical_list: D055419:Bone Morphogenetic Protein 7; D001205:Ascorbic Acid