title: Transplantation of oligodendrocyte precursor cells improves locomotion deficits in rats with spinal cord irradiation injury.
abstract: Demyelination contributes to the functional impairment of irradiation injured spinal cord. One potential therapeutic strategy involves replacing the myelin-forming cells. Here, we asked whether transplantation of Olig2(+)-GFP(+)-oligodendrocyte precursor cells (OPCs), which are derived from Olig2-GFP-mouse embryonic stem cells (mESCs), could enhance remyelination and functional recovery after spinal cord irradiation injury. We differentiated Olig2-GFP-mESCs into purified Olig2(+)-GFP(+)-OPCs and transplanted them into the rats' cervical 4-5 dorsal spinal cord level at 4 months after irradiation injury. Eight weeks after transplantation, the Olig2(+)-GFP(+)-OPCs survived and integrated into the injured spinal cord. Immunofluorescence analysis showed that the grafted Olig2(+)-GFP(+)-OPCs primarily differentiated into adenomatous polyposis coli (APC(+)) oligodendrocytes (54.6±10.5%). The staining with luxol fast blue, hematoxylin & eosin (LFB/H&E) and electron microscopy demonstrated that the engrafted Olig2(+)-GFP(+)-OPCs attenuated the demyelination resulted from the irradiation. More importantly, the recovery of forelimb locomotor function was enhanced in animals receiving grafts of Olig2(+)-GFP(+)-OPCs. We concluded that OPC transplantation is a feasible therapy to repair the irradiated lesions in the central nervous system (CNS).
authors: Department of Anatomy, Histology and Embryology, Shanghai Medical College, Fudan University, Shanghai, China.
authors: Yan
authors: Y
authors: Sun
authors: Chong-Chong
authors: CC
authors: Xu
authors: Jin
authors: J
authors: Li
authors: Xi-Yin
authors: XY
authors: Guan
authors: Lu
authors: L
authors: Gao
authors: Li-Xiang
authors: LX
authors: Ma
authors: Rui-Xi
authors: RX
authors: Li
authors: Yu-Wen
authors: YW
authors: Peng
authors: Guo-Pei
authors: GP
authors: Zhu
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology / Q000648:ultrastructure; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D048430:Cell Shape; D002470:Cell Survival; D003711:Demyelinating Diseases / Q000150:complications / Q000503:physiopathology / Q000628:therapy; D005260:Female; D005552:Forelimb / Q000503:physiopathology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008124:Locomotion / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology / Q000637:transplantation*; D011832:Radiation Injuries / Q000150:complications / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000473:pathology / Q000528:radiation effects; D013119:Spinal Cord Injuries / Q000150:complications / Q000503:physiopathology* / Q000628:therapy*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D049452:Green Fluorescent Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; C512773:Olig2 protein, mouse