title: IL-8 -251T/A and IL-12B 1188A/C polymorphisms are associated with gout in a Chinese male population.
abstract: OBJECTIVE
  Gout is caused by monosodium urate (MSU) crystal-induced inflammation of the joints and periarticular tissues. MSU crystals activate NALP3 and mediate interleukin (IL)-1β generation from its inactive pro-form, resulting in cellular activation and an IL-8-mediated neutrophil influx into the joint. IL-8 and IL-12 are important chemokines related to the initiation and amplification of acute and chronic inflammatory processes. In this study, we investigated whether the IL-8 -251T/A and IL-12 1188A/C polymorphisms are associated with susceptibility to gout in a Chinese Han male population.
  
  
  METHODS
  Overall, 387 patients with gout and 576 controls were included in this study. Genotyping was performed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). An association analysis was carried out using the χ2 test. A genotype-phenotype analysis was also conducted.
  
  
  RESULTS
  The T allele of IL-8 -251 was associated with risk of gout [p = 0.031 (odds ratio (OR) 1.229, 95% confidence interval (CI) 1.019-1.483]. There was a clear link between the IL-12 1188 AA and AC genotypic and A allelic frequencies between gout cases and controls (p < 0.001, df = 2 by genotype; p < 0.001, OR 1.404, 95% CI 1.165-1.691 by allele).
  
  
  CONCLUSIONS
  Our results suggest that the IL-8 -251T/A and IL-12B 1188A/C polymorphisms may be relevant host susceptibility factors for the development of gout.
authors: Shandong Provincial Key Laboratory of Metabolic Disease, Qingdao University, Qingdao, PR China.
authors: S
authors: S
authors: Liu
authors: C
authors: C
authors: Yin
authors: N
authors: N
authors: Chu
authors: L
authors: L
authors: Han
authors: C
authors: C
authors: Li
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers / Q000737:chemistry; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006073:Gout / Q000235:genetics*; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D011795:Surveys and Questionnaires
chemical_list: C508606:CXCL8 protein, human; D017931:DNA Primers; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40; D016209:Interleukin-8