title: First evidence of the possible implication of the 11-deoxycorticosterone (DOC) in immune activity of Eurasian perch (Perca fluviatilis, L.): comparison with cortisol.
abstract: Cortisol, the main corticosteroid in fish, is frequently described as a modulator of fish immune system. Moreover, 11-deoxycorticosterone (DOC) was shown to bind and transcriptionally activate the mineralocorticoid receptor and may act as a mineralocorticoid in fish. Immune modulations induced by intraperitoneal injections of these two corticosteroids were assessed in Eurasian perch juveniles. Cortisol and DOC were injected at 0.8 mg kg(-1) and 0.08 mg kg(-1) body weight respectively. Cortisol increased plasma lysozyme activity 72 h post-injection, C-type lysozyme expression in spleen from 1 to 72 h post-injection, and favoured blood neutrophils at the expense of a mixture of lymphocytes and thrombocytes. Moreover, 6 h after injection, cortisol reduced expression levels of the pro-inflammatory cytokine TNF-α in spleen. DOC had no effects on the immune variables measured in plasma, but increased expression levels of C-type lysozyme and apolipoprotein A1 mRNA in both gills and spleen. Meanwhile, DOC stimulated its putative signalling pathway by increasing expression of mineralocorticoid receptor and 11β-hydroxysteroid dehydrogenase-2 in spleen. These results confirmed the role of cortisol as an innate, short term immune stimulator. For the first time, DOC is described as a possible immune stimulator in fish.
authors: Research Unit in Environmental and Evolutionary Biology (URBE), University of Namur (FUNDP), Rue de Bruxelles 61, B-5000 Namur, Belgium. cedric.mathieu@fundp.ac.be
authors: Cédric
authors: C
authors: Mathieu
authors: Sylvain
authors: S
authors: Milla
authors: S N M
authors: SN
authors: Mandiki
authors: Jessica
authors: J
authors: Douxfils
authors: Caroline
authors: C
authors: Douny
authors: Marie-Louise
authors: ML
authors: Scippo
authors: Edwin
authors: E
authors: De Pauw
authors: Patrick
authors: P
authors: Kestemont
mesh_terms: D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003900:Desoxycorticosterone / Q000008:administration & dosage / Q000494:pharmacology*; D029941:Fish Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D009113:Muramidase / Q000097:blood; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D010471:Perches / Q000097:blood / Q000235:genetics* / Q000276:immunology; D018161:Receptors, Mineralocorticoid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D016632:Apolipoprotein A-I; D018076:DNA, Complementary; D029941:Fish Proteins; D018161:Receptors, Mineralocorticoid; D014409:Tumor Necrosis Factor-alpha; D003900:Desoxycorticosterone; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2; D009113:Muramidase; D006854:Hydrocortisone