title: Biodegradable porous sheet-like scaffolds for soft-tissue engineering using a combined particulate leaching of salt particles and magnetic sugar particles.
abstract: Scaffolds serving as artificial extracellular matrixes (ECMs) play a pivotal role in the process of tissue regeneration by providing optimal cellular environments for penetration, ingrowth, and vascularization. Stacks of sheet-like scaffold can be engineered to become artificial ECMs, suggesting a great potential for achieving complex 3-D tissue regeneration to support cell survival and growth. In this study, we proposed and investigated a combined particulate leaching of magnetic sugar particles (MSPs) and salt particles for the development of a sheet-like scaffold. MSPs were fabricated by encapsulating NdFeB particles inside sugar spheres and were controlled using magnetic fields as a porogen to control pore size, pore structure and pore density while fabricating the scaffold. We studied the influence of the strength of the magnetic fields in controlling the coating thickness of the unmagnetized MSPs during the fabrication of the sheet-like scaffolds. The experimental relationship between magnetic flux density and the thickness of the MSP layer was illustrated. Furthermore, we investigated the infiltration capacity of different concentrations of poly(L-lactide-co-ɛ-caprolactone) (PLCL) as a scaffold material on MSP clusters. Following polymer casting and removal of the sugar template, spherical pores were generated inside the scaffolds. Cultivation of NIH/3T3 fibroblasts on the fabricated scaffold proves that the proposed method can be applied in the cell sheet fabrication.
authors: Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. huchengz@gmail.com
authors: Chengzhi
authors: C
authors: Hu
authors: Carlos
authors: C
authors: Tercero
authors: Seiichi
authors: S
authors: Ikeda
authors: Masahiro
authors: M
authors: Nakajima
authors: Hirotaka
authors: H
authors: Tajima
authors: Yajing
authors: Y
authors: Shen
authors: Toshio
authors: T
authors: Fukuda
authors: Fumihito
authors: F
authors: Arai
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002448:Cell Adhesion; D060328:Magnetic Phenomena; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters; D016062:Porosity; D012492:Salts; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D002241:Carbohydrates; D011091:Polyesters; D012492:Salts; C062968:lactide-caprolactone copolymer