title: Transfection of mammalian cells using block copolypeptide vesicles.
abstract: An arginine-leucine block copolypeptide (R60 L20 ) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60 L20 vesicles also possess the ability to deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, the R60 L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity. This behavior is potentially due to its stronger interaction with DNA which subsequently provides better protection against anionic heparin.
authors: Department of Bioengineering, University of California, Los Angeles, CA 90095, USA.
authors: Victor Z
authors: VZ
authors: Sun
authors: Uh-Joo
authors: UJ
authors: Choe
authors: April R
authors: AR
authors: Rodriguez
authors: Howard
authors: H
authors: Dai
authors: Timothy J
authors: TJ
authors: Deming
authors: Daniel T
authors: DT
authors: Kamei
mesh_terms: D000818:Animals; D000838:Anions; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008027:Light; D008055:Lipids; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D010455:Peptides / Q000494:pharmacology*; D010957:Plasmids / Q000378:metabolism; D012542:Scattering, Radiation; D014162:Transfection / Q000379:methods*; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D000838:Anions; D015850:Interleukin-6; D008055:Lipids; C086724:Lipofectamine; D008164:Luminescent Proteins; D010455:Peptides; D053835:Unilamellar Liposomes; C413663:fluorescent protein 583; D004247:DNA; D003850:Deoxyribonuclease I