title: Thin-layer chromatography-densitometry analysis of dimethoxyphenylbutadiene content in Zingiber cassumunar rhizomes.
abstract: Zingiber cassumunar (Zingiberaceae) has been widely used as a traditional medicine in southeast Asia, especially Thailand, for treatment of asthma, inflammation, and joint pain. (E)-4-(3,4-dimethoxyphenyl)butadiene, or DMPBD, a major component in the rhizome, has been reported to be an active anti-inflammatory agent. This research developed a TLC-densitometry method for the simultaneous quantification of DMPBD in the rhizome extracts of four varieties of Z. cassumunar. DMPBD (dimethoxyphenylbutadiene) was found in the range of 0.90 +/- 0.01 to 1.61 +/- 0.05% (w/w). [corrected] The method was validated for linearity, precision, accuracy, robustness, LOD, and LOQ. The linearity was indicated by a good regression coefficient 0.9940 and covered the range of 130 to 703 ng/band. Intraday and interday precision (RSD) were less than 2%. Accuracy of the method was evaluated by a recovery study conducted at three different levels; the average recovery was 103.1%. The LOD and LOQ were 10 and 40 ng, respectively. The proposed TLC-densitometry method was simple, precise, specific, sensitive, and inexpensive. This method can be used for routine QC of raw materials of Z. cassumunar rhizomes, extracts, and its products, especially Prasaplai, a Thai traditional medicine for relieving dysmenorrhea.
authors: Rangsit University, Faculty of Oriental Medicine, 52/347 Pahonyothin Rd, Pathumthani 12000, Thailand. pywgs@mahidol.ac.th
authors: Prasan
authors: P
authors: Tangyuenyongwatana
authors: Vichien
authors: V
authors: Keeratinijakal
authors: Wandee
authors: W
authors: Gritsanapani
mesh_terms: D002070:Butadienes / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D007202:Indicators and Reagents; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012996:Solutions; D013785:Thailand; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D002070:Butadienes; D007202:Indicators and Reagents; D010936:Plant Extracts; D012996:Solutions