title: In vivo genotoxicity and oxidative stress evaluation of an ethanolic extract from piquiá (Caryocar villosum) pulp.
abstract: In this study, the ethanolic extract obtained from piquiá pulp was assessed for genotoxicity and oxidative stress by employing the micronucleus test in bone marrow and peripheral blood cells in addition to comet, thiobarbituric-acid-reactive substances (TBARS), and reduced glutathione assays in the liver, kidney, and heart. Additionally, phytochemical analyses were performed to identify and quantify the chemical constituents of the piquiá extract. Wistar rats were treated by gavage with an ethanolic extract from piquiá pulp (75 mg/kg body weight) for 14 days, and 24 h prior to euthanasia, they received an injection of saline or doxorubicin (15 mg/kg body weight, intraperoneally). The results demonstrated that piquiá extract at the tested dose was genotoxic but not mutagenic, and it increased the TBARS levels in the heart. Further studies are required to fully elucidate how the properties of ethanolic extract of piquiá pulp can affect human health.
authors: Department of Clinical Analysis, Toxicology, and Food Science, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. mararibio2005@yahoo.com.br
authors: Mara Ribeiro
authors: MR
authors: Almeida
authors: Alexandre Ferro
authors: AF
authors: Aissa
authors: Tarsila Daysy
authors: TD
authors: Ursula Hermogenes Gomes
authors: Joana D'Arc Castania
authors: JD
authors: Darin
authors: Renan Campos
authors: RC
authors: Chisté
authors: Adriana Zerlotti
authors: AZ
authors: Mercadante
authors: Lusânia Maria Greggi
authors: LM
authors: Antunes
authors: Maria Lourdes Pires
authors: ML
authors: Bianchi
mesh_terms: D000818:Animals; D002417:Cattle; D020552:Comet Assay; D020448:Ericales / Q000009:adverse effects*; D005638:Fruit / Q000009:adverse effects*; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000009:adverse effects*; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D010936:Plant Extracts / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D009153:Mutagens; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione