title: Simultaneous detection of oseltamivir- and amantadine-resistant influenza by oligonucleotide microarray visualization.
abstract: Presently, the resistance of Influenza A virus isolates causes great difficulty for the prevention and treatment of influenza A virus infection. It is important to establish a drug-resistance detection method for epidemiological study and personalized medicine in the clinical setting. Consequently, a cost-effective oligonucleotide microarray visualization method, which was based on quantum dot-catalyzed silver deposition, was developed and evaluated for the simultaneous detection of neuraminidase H275Y and E119V; matrix protein 2 V27A and S31N mutations of influenza A (H3N2), seasonal influenza A (H1N1), and 2009 influenza A (H1N1). Then, 307 clinical throat swab specimens were detected and the drug-resistance results showed that 100% (17/17) of influenza A (H3N2) and 100% (259/259) of 2009 influenza A (H1N1) samples were resistant to amantadine and susceptible to oseltamivir; and 100% (5/5) of seasonal influenza A (H1N1) samples were resistant to both amantadine and oseltamivir.
authors: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.
authors: Yingjie
authors: Y
authors: Zhang
authors: Qiqi
authors: Q
authors: Liu
authors: Dou
authors: D
authors: Wang
authors: Suhong
authors: S
authors: Chen
authors: Shengqi
authors: S
authors: Wang
mesh_terms: D000547:Amantadine / Q000494:pharmacology / Q000627:therapeutic use*; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001483:Base Sequence; D003362:Cost-Benefit Analysis; D017931:DNA Primers; D024882:Drug Resistance, Viral; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000235:genetics; D007251:Influenza, Human / Q000188:drug therapy* / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D053139:Oseltamivir / Q000494:pharmacology / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction
chemical_list: D000998:Antiviral Agents; D017931:DNA Primers; D053139:Oseltamivir; D000547:Amantadine