title: [Determination of capsaicinoids and eugenol in waste-edible-oil by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry].
abstract: A method was developed for the determination of capsaicinoids (capsaicin, dihydrocapsaicin and synthetic capsaicin) and eugenol in waste-edible-oil extracted by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The capsaicinoids and eugenol in waste-edible-oil were extracted by methanol, and then separated by a SUPEL COSIL ABZ + Plus dC18 column (150 mm x4.6 mm, 5 microm). The analysis was performed by MS/MS with electrospray ionization in positive and negative ion modes with multiple reaction monitoring (MRM). The limits of detection for capsaicin, dihydrocapsaicin, synthetic capsaicin and eugenol were 0.02, 0.03, 0.03 and 0.6 microg/L, respectively. The good linear relationships were obtained in certain concentration ranges of capsaicinoids and eugenol. The relative standard deviations (RSDs, n=5) of same-worker and different-worker were less than 5%. The method is exclusive, sensitive and accurate, and can be used in waste-edible-oil determination.
authors: Material Evidence Identiication Center of Chongqing Public Security Bureau, Chongqing 400021, China. zhangzhong5123@126.com
authors: Zhong
authors: Z
authors: Zhang
authors: Fei
authors: F
authors: Ren
authors: Pan
authors: P
authors: Zhang
mesh_terms: D002211:Capsaicin / Q000031:analogs & derivatives / Q000032:analysis* / Q000302:isolation & purification; D002212:Capsicum / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D003296:Cooking; D005054:Eugenol / Q000032:analysis*; D059625:Liquid-Liquid Extraction / Q000379:methods; D009821:Oils / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009821:Oils; D005054:Eugenol; D002211:Capsaicin; C012906:dihydrocapsaicin