title: Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria.
abstract: Lactic acid bacteria (LAB) produce homopolysaccharides (HoPS) and heteropolysaccharides (HePS) with potential functional properties. In this work, we have performed a comparative analysis of production and purification trials of these biopolymers from bacterial culture supernatants. LAB strains belonging to four different genera, both natural as well as recombinant, were used as model systems for the production of HoPS and HePS. Two well characterized strains carrying the gft gene were used for β-glucan production, Pediococcus parvulus 2.6 (P. parvulus 2.6) isolated from cider, and the recombinant strain Lactococcus lactis NZ9000[pGTF] (L. lactis NZ9000[pGTF]). In addition, another cider isolate, Lactobacillus suebicus CUPV225 (L. suebicus CUPV225), and Leuconostoc mesenteroides RTF10 (L. mesenteroides RTF10), isolated from meat products were included in the study. Chemical analysis of the EPS revealed that L. mesenteroides produces a dextran, L. suebicus a complex heteropolysaccharide, and the β-glucan producing-strains the expected 2-substituted (1,3)-β-glucan.
authors: Department of Molecular Microbiology and Infection Biology, Centro de Investigaciones Biológicas, Spanish Council for Scientific Research (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain. sikelia@cib.csic.es
authors: Sara
authors: S
authors: Notararigo
authors: Montserrat
authors: M
authors: Nácher-Vázquez
authors: Idoia
authors: I
authors: Ibarburu
authors: Ma Laura
authors: ML
authors: Werning
authors: Pilar Fernández
authors: PF
authors: de Palencia
authors: Ma Teresa
authors: MT
authors: Dueñas
authors: Rosa
authors: R
authors: Aznar
authors: Paloma
authors: P
authors: López
authors: Alicia
authors: A
authors: Prieto
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media / Q000737:chemistry; D003911:Dextrans / Q000096:biosynthesis; D005285:Fermentation; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D007934:Leuconostoc / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D008745:Methylation; D046529:Microscopy, Electron, Transmission; D010376:Pediococcus / Q000737:chemistry / Q000235:genetics; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D013055:Spectrophotometry, Infrared; D047071:beta-Glucans / Q000737:chemistry
chemical_list: D003470:Culture Media; D003911:Dextrans; D011135:Polysaccharides, Bacterial; D047071:beta-Glucans