title: [Significance of miR-155, miR-34a and miR-30a expression in diffuse large B-cell lymphoma].
abstract: OBJECTIVE
  To determine the expressions of miR-155, miR-34a and miR-30a in diffuse large B-cell lymphoma (DLBCL) and to explore their potential correlation with clinicopathological characteristics.
  
  
  METHODS
  The expression level of miR-155, miR-34a and miR-30a in 46 DLBCL samples were determined with TaqMan real-time polymerase chain reaction. Interphase fluorescence in situ hybridization (I-FISH) was performed to detect MYC and p53 genes' status, and immunohistochemistry (Envision method) was used to evaluate the expression of CD3, CD10, CD20, BCL-6 and MUM-1 in DLBCL. The DLBCLs were classified into germinal center B cell-like (GCB) and non germinal center B cell-like (non-GCB) subtypes according to Hans' criteria.
  
  
  RESULTS
  Compared with normal controls, miR-155 expression level was significantly higher in DLBCL. The expression level of miR-155 in non-GCB type was higher than that in GCB type. It was shown that the patients with MYC rearrangement had lower expression level of miR-155 than the negative controls. Compared with p53 normal group, the expression level of miR-34a was significantly lower in p53 deletion group. It was also shown that the patients with BCL-6 protein expression had lower expression of miR-30a compared with the negative group.
  
  
  CONCLUSIONS
  miR-155 expression level is different in normal controls, DLBCL and patients with subtype DLBCL. It therefore has a diagnosis value for DLBCL. miR-34a is of great prognostic significance. miR-155, miR-34a and miR-30a may be potential therapy targets for DLBCL.
authors: Department of Hematology, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, People's Republic of China.
authors: Guan-xing
authors: GX
authors: Sun
authors: Xiang-shan
authors: XS
authors: Cao
authors: Qing
authors: Q
authors: Li
authors: Zhilin
authors: Z
authors: Wang
authors: Jing
authors: J
authors: Peng
authors: Chang-qing
authors: CQ
authors: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C524942:MIRN155 microRNA, human; C546086:MIRN30b microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53