title: Molecular cloning and characterization of cat, gpx1 and Cu/Zn-sod genes in pengze crucian carp (Carassius auratus var. Pengze) and antioxidant enzyme modulation induced by hexavalent chromium in juveniles.
abstract: Hexavalent chromium (Cr(6+)) is a common pollutant transient metal with high toxicity in the environment. The toxicological effects partly result from oxidative damage due to the production of excessive reactive oxygen species (ROS) in the reductive process of Cr(6+). To explore the influence of ROS induced directly by Cr(6+) on the oxidative stress generation and antioxidant system, the full length cDNAs of antioxidant-related genes cat, gpx1 and Cu/Zn-sod were successfully acquired from pengze crucian carp first and analyzed. Furthermore, the mRNA expression of the antioxidant genes encompassing catalase (cat), copper/zinc superoxide dismutase (Cu/Zn-sod) and glutathione peroxidase (gpx1), antioxidant enzyme activities of CAT, SOD, and GPx and total protein content were further studied in the gill, intestine and liver of pengze crucian carp (Carassius auratus var. Pengze) juveniles upon acute exposure to Cr(6+) at concentrations of 0.1, 1.0, 10 and 100 mg/L for 4 days. Differential significant changes of the antioxidant enzymes and gene expression were observed in different tissues. The findings contribute to better understanding the antioxidant mechanisms induced by Cr(6+) and selecting the organic-specific sensitive biomarkers to monitor the safety of the aquatic ecosystem.
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors: Meng
authors: M
authors: Li
authors: Yao
authors: Y
authors: Zheng
authors: Hongwei
authors: H
authors: Liang
authors: Linhu
authors: L
authors: Zou
authors: Jiejie
authors: J
authors: Sun
authors: Yingying
authors: Y
authors: Zhang
authors: Fang
authors: F
authors: Qin
authors: Shaozhen
authors: S
authors: Liu
authors: Zaizhao
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D002857:Chromium / Q000633:toxicity*; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D006054:Goldfish / Q000235:genetics* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D014874:Water Pollutants, Chemical; D002857:Chromium; C074702:chromium hexavalent ion; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase