title: Evaluation of efficacy of 18 strains of entomopathogenic nematodes (Rhabditida) against Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) under laboratory conditions.
abstract: Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) is an important plant virus vector in grapevine crops in Brazil and other countries. The mealybug grows in roots and leaves of the grapes. Entomopathogenic nematodes (EPNs) are efficient control agents against insects associated to the soil and could be applied with the same equipment used for chemical insecticides. The aim of this study was to select effective EPNs for controlling P. citri females in laboratory conditions (25±1°C, UR 60±10%). We tested 17 native [Steinernema rarum (6 strains), Steinernema glaseri, Steinernema feltiae, Steinernema riobrave, Steinernema sp., Heterorhabditis bacteriophora (7 strains)] and only one exotic strain (Steinernema carpocapsae ALL). The bioassays were done on Petri dishes infested with females of P. citri, which were sprayed with EPNs juveniles. The strain with larger pathogenicity and virulence in laboratory was H. bacteriophora RS33 (from 69.0% to 92.2% of mortality), native of Rio Grande do Sul.
authors: Federal University of Alagoas, Chemistry Institute, Maceió, AL, Brazil.
authors: Carla Ruth de Carvalho
authors: CR
authors: Barbosa Negrisoli
authors: Aldomario Santo
authors: AS
authors: Negrisoli Júnior
authors: Marcos
authors: M
authors: Botton
authors: Mauro Silveira
authors: MS
authors: Garcia
authors: Daniel
authors: D
authors: Bernardi
mesh_terms: D000818:Animals; D001681:Biological Assay; D001938:Brazil; D005260:Female; D007303:Insect Vectors / Q000469:parasitology* / Q000821:virology; D007928:Lethal Dose 50; D010572:Pest Control, Biological / Q000379:methods*; D058643:Planococcus Insect / Q000469:parasitology*; D010935:Plant Diseases / Q000517:prevention & control / Q000821:virology; D018515:Plant Leaves / Q000469:parasitology; D018517:Plant Roots / Q000469:parasitology; D010942:Plant Viruses / Q000502:physiology; D011897:Random Allocation; D017168:Rhabditida / Q000472:pathogenicity*; D014774:Virulence; D027843:Vitis / Q000469:parasitology* / Q000821:virology