title: [Immunomodulating effect of an Ixodes persulcatus (Ixodidae) tick salivary gland extract on BALB/c mice lymphocytes in an in vitro system].
abstract: The immunomodulating effect of the components of an Ixodes persulcatus (Ixodidae) tick salivary gland extract (SGE) on BALB/c mice lymphocytes was evaluated. SGE of partially engorged ticks at a concentration of 50 microg/ml causes the maximum suppression ofT- and B-lymphocyte subpopulations. SGE of hungry ticks at the same concentration induces the suppression of only CD69+ T cells and TLR-2+ B cells, but produces no suppressive effect on CD69+ B lymphocytes, TLR-2+ T lymphocytes, and TLR-4+ T and B lymphocytes. SGE shows different effects on the synthesis of IFN-gamma and IL-4 by T helper cells. SGE of hungry ticks stimulated the increase of IFN-gamma and IL-4 synthesis by 4.7 and 2.6 times, respectively, as compared to the control. The findings may be of value in studying the pathogenesis of transmissible infections and in designing the vaccines based on tick gland components.
authors: E V
authors: EV
authors: Zyrina
authors: V V
authors: VV
authors: Firstova
authors: A V
authors: AV
authors: Shtannikov
authors: G M
authors: GM
authors: Titareva
authors: V P
authors: VP
authors: Gutova
authors: I S
authors: IS
authors: Vasil'eva
authors: S F
authors: SF
authors: Biketov
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D015870:Gene Expression; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology; D018884:Ixodes / Q000737:chemistry*; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012469:Salivary Glands / Q000737:chemistry*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014020:Tissue Extracts / Q000276:immunology / Q000494:pharmacology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007155:Immunologic Factors; D007371:Interferon-gamma; D016753:Interleukin-10; D015847:Interleukin-4; D037181:Lectins, C-Type; D014020:Tissue Extracts; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D000099192:CD69 Antigens; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse