title: A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis.
abstract: Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy® and a polymerase chain reaction (PCR) assay. Overall, 102 samples from HSCT patients and 128 from children obtained positivity rate of 18.6% and 14.1% respectively. PCR sensitivity was highest mainly on samples collected after five days of symptoms onset. A combination of both DFA and reverse transcriptase-PCR methods for HSCT high-risk patients is the best diagnostic flow for HRSV diagnosis among these patients.
authors: Disciplina de Infectologia, Laboratório de Virologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: Luciana Peniche
authors: LP
authors: Moreira
authors: Aripuanã Sakurada Aranha
authors: AS
authors: Watanabe
authors: Emerson
authors: E
authors: Carraro
authors: Ellen Ricci Monteiro
authors: ER
authors: da Silva
authors: Sandra Baltazar
authors: SB
authors: Guatura
authors: Celso
authors: C
authors: Granato
authors: Nancy Junqueira
authors: NJ
authors: Bellei
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D019085:Fluorescent Antibody Technique, Direct; D019337:Hematologic Neoplasms / Q000601:surgery; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000453:epidemiology; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D012367:RNA, Viral