title: Chemical characterisation and determination of sensory attributes of hydrolysates produced by enzymatic hydrolysis of whey proteins following a novel integrative process.
abstract: The overall aim of this work was to characterise the major angiotensin-converting enzyme (ACE) inhibitory peptides produced by enzymatic hydrolysis of whey proteins, through the application of a novel integrative process. This process consisted of the combination of adsorption and microfiltration within a stirred cell unit for the selective immobilisation of β-lactoglobulin and casein-derived peptides (CDP) from whey. The adsorbed proteins were hydrolysed in situ, which resulted in the separation of peptide products from the substrate and fractionation of peptides. Two different hydrolysates were produced: (i) from CDP (IC(50)=287 μg/mL) and (ii) from β-lactoglobulin (IC(50)=128 μg/mL). The well-known antihypertensive peptide IPP and several novel peptides that have structural similarities with reported ACE inhibitory peptides were identified and characterised in both hydrolysates. Furthermore, the hydrolysates were assessed for bitterness. No significant difference was found between the bitterness of the control (milk with no hydrolysate) and hydrolysate samples at different concentrations (at, below and above the IC(50)).
authors: Department of Food and Nutrition Sciences, The University of Reading, Reading, United Kingdom.
authors: Fisseha Tesfay
authors: FT
authors: Welderufael
authors: Trevor
authors: T
authors: Gibson
authors: Lisa
authors: L
authors: Methven
authors: Paula
authors: P
authors: Jauregi
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D000959:Antihypertensive Agents / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry; D008894:Milk Proteins / Q000737:chemistry*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification; D011492:Protein Hydrolysates / Q000737:chemistry*; D013649:Taste; D000067816:Whey Proteins
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D008894:Milk Proteins; D010455:Peptides; D011492:Protein Hydrolysates; D000067816:Whey Proteins