title: The ubiquitin-fold modifier 1 (Ufm1) cascade of Caenorhabditis elegans.
abstract: Ufm1 (ubiquitin-fold modifier 1) is the most recently identified member of the ubiquitin-like protein family. We characterized the Ufm1 cascade of the model organism Caenorhabditis elegans in terms of function and analyzed interactions of the involved proteins in vitro and in vivo. Furthermore, we investigated the phenotypes of the deletion mutants uba5(ok3364) (activating enzyme of Ufm1) and ufc1(tm4888) (conjugating enzyme of Ufm1). The viable deletion mutants showed a decrease in reproduction, development, life span, and a reduced survival under heavy metal stress. However, an increased survival rate under pathogenic, oxidative, heat, and endoplasmic reticulum stress was observed. We propose that the Ufm1 cascade negatively regulates the IRE1-mediated unfolded protein response.
authors: Department of Molecular Physiology, Institute for Animal Physiology, University of Muenster, Schlossplatz 8, 48143 Muenster, Germany.
authors: Patrick
authors: P
authors: Hertel
authors: Jens
authors: J
authors: Daniel
authors: Dirk
authors: D
authors: Stegehake
authors: Hannah
authors: H
authors: Vaupel
authors: Sareetha
authors: S
authors: Kailayangiri
authors: Clio
authors: C
authors: Gruel
authors: Christian
authors: C
authors: Woltersdorf
authors: Eva
authors: E
authors: Liebau
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D018869:Heat-Shock Response / Q000502:physiology; D008136:Longevity / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology; D012098:Reproduction / Q000502:physiology; D056811:Unfolded Protein Response / Q000502:physiology
chemical_list: D029742:Caenorhabditis elegans Proteins