title: DNA delivery via cationic solid lipid nanoparticles (SLNs).
abstract: In recent years the use of solid lipid nanoparticles (SLNs) as transport systems for the delivery of drugs and biomolecules has become particularly important. The use of cationic SLNs developed by the technique of microemulsion, which are complexed with DNA in order to study their application as non-viral vectors in gene therapy, is reported. The nanoparticles are characterized by scanning electron microscopy and transmission electron microscopy (SEM and TEM), atomic force microscopy (AFM) and differential scanning calorimetry (DSC). Furthermore, the process of lyophilization of the samples and their stability was studied. The nanoparticles obtained presented a particle size of 340 nm with a positive surface charge of 44 mV and the capability of forming lipoplexes with DNA plasmids was stated.
authors: Faculty of Pharmacy, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. carolinacarrillo@ub.edu
authors: Carolina
authors: C
authors: Carrillo
authors: Noemí
authors: N
authors: Sánchez-Hernández
authors: Encarna
authors: E
authors: García-Montoya
authors: Pilar
authors: P
authors: Pérez-Lozano
authors: Josep M
authors: JM
authors: Suñé-Negre
authors: Josep R
authors: JR
authors: Ticó
authors: Carlos
authors: C
authors: Suñé
authors: Montserrat
authors: M
authors: Miñarro
mesh_terms: D000588:Amines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004075:Diglycerides / Q000737:chemistry*; D005612:Freeze Drying; D018014:Gene Transfer Techniques; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010957:Plasmids; D013499:Surface Properties
chemical_list: D000588:Amines; D004075:Diglycerides; D004247:DNA; C009317:stearylamine; C009032:precirol