title: Screening of high α-arbutin producing strains and production of α-arbutin by fermentation.
abstract: A mutant Xanthomonas maltophilia BT-112 with high α-anomer-selective glycosylation activity was screened by a series of mutation methods including UV light, N-methyl-N-nitro-N-nitroso-guanidine treatment and quick neutron mutation. The α-arbutin titer increased 15-folds compared with the parent strain. The optimal conditions for culture medium and the operational conditions for lab-scale fermenter were investigated. Under optimized conditions, the maximal hydroquinone (HQ) tolerance of cells and yield of α-arbutin were 120 mM and 30.6 g/l, respectively. The molar conversion yield of α-arbutin based on the amount of HQ supplied reached 93.6 %. The product was identified as α-arbutin by (13)C NMR and (1)H NMR analysis. In conclusion, the results in this work provide a one-step and cost-effective method for the large-scale production of α-arbutin.
authors: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. liuchunqiao010@yahoo.com.cn
authors: Chun-Qiao
authors: CQ
authors: Liu
authors: Li
authors: L
authors: Deng
authors: Peng
authors: P
authors: Zhang
authors: Shu-Rong
authors: SR
authors: Zhang
authors: Luo
authors: L
authors: Liu
authors: Tao
authors: T
authors: Xu
authors: Fang
authors: F
authors: Wang
authors: Tian-Wei
authors: TW
authors: Tan
mesh_terms: D001104:Arbutin / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D006031:Glycosylation; D020615:Stenotrophomonas maltophilia / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D001104:Arbutin