title: Analytical determination of bioactive compounds as an indication of fruit quality.
abstract: The aim of this investigation was to determine the bioactive compounds in kiwifruit as an indication of quality after extraction using methanol and ethyl acetate. Using FTIR and three-dimensional fluorescence spectroscopy and electrospray ionization/MS, the contents of polyphenols, flavonoids, flavanols, and tannins, and the level of the antioxidant activity by 2, 2-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt, 1, 1-diphenyl-2-picrylhydrazyl, ferric-reducing/ antioxidant power, and cupric reducing antioxidant capacity assays were determined and compared. It was found that the methanol extracts of kiwifruit showed significantly higher amounts of bioactive acetate extracts. The cultivar Bidan, in comparison compounds and antioxidant activities than the ethyl with the classic Hayward, showed significantly higher bioactivity. For the first time, Bidan organic kiwifruit was analyzed for its antioxidant activities and compared with the widely consumed Hayward organic based on its bioactive compounds and fluorescence properties. Relatively high content of bioactive compounds and positive antioxidant and antiproliferative properties of kiwifruit determined by the advanced analytical methods justify its use as a source of valuable antioxidants. The methods used are applicable for bioactivity determination, in general, for any food products.
authors: Mokpo National University, Department of Horticultural Science, Muan, Jeonnam, South Korea.
authors: Yong Seo
authors: YS
authors: Park
authors: Buk-Gu
authors: BG
authors: Heo
authors: Kyung-Sik
authors: KS
authors: Ham
authors: Seong-Gook
authors: SG
authors: Kang
authors: Yang-Kyun
authors: YK
authors: Park
authors: Alina
authors: A
authors: Nemirovski
authors: Zeev
authors: Z
authors: Tashma
authors: Shela
authors: S
authors: Gorinstein
authors: Hanna
authors: H
authors: Leontowicz
authors: Maria
authors: M
authors: Leontowicz
mesh_terms: D029042:Actinidia / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry; D000975:Antioxidants / Q000032:analysis; D001205:Ascorbic Acid / Q000032:analysis; D052160:Benzothiazoles / Q000032:analysis; D001713:Biphenyl Compounds / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002839:Chromans / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D019327:Copper Sulfate / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D044948:Flavonols / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D007202:Indicators and Reagents; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis; D059808:Polyphenols / Q000032:analysis; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000032:analysis; D013634:Tannins / Q000032:analysis; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D002839:Chromans; D004396:Coloring Agents; D005290:Ferric Compounds; D005419:Flavonoids; D044948:Flavonols; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D013451:Sulfonic Acids; D013634:Tannins; D013778:Tetrazolium Salts; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; C022616:thiazolyl blue; D019327:Copper Sulfate; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid