title: A novel role for Arabidopsis CBL1 in affecting plant responses to glucose and gibberellin during germination and seedling development.
abstract: Glucose and phytohormones such as abscisic acid (ABA), ethylene, and gibberellin (GA) coordinately regulate germination and seedling development. However, there is still inadequate evidence to link their molecular roles in affecting plant responses. Calcium acts as a second messenger in a diverse range of signal transduction pathways. As calcium sensors unique to plants, calcineurin B-like (CBL) proteins are well known to modulate abiotic stress responses. In this study, it was found that CBL1 was induced by glucose in Arabidopsis. Loss-of-function mutant cbl1 exhibited hypersensitivity to glucose and paclobutrazol, a GA biosynthetic inhibitor. Several sugar-responsive and GA biosynthetic gene expressions were altered in the cbl1 mutant. CBL1 protein physically interacted with AKINβ1, the regulatory β subunit of the SnRK1 complex which has a central role in sugar signaling. Our results indicate a novel role for CBL1 in modulating responses to glucose and GA signals.
authors: Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, China.
authors: Zhi-Yong
authors: ZY
authors: Li
authors: Zhao-Shi
authors: ZS
authors: Xu
authors: Yang
authors: Y
authors: Chen
authors: Guang-Yuan
authors: GY
authors: He
authors: Guang-Xiao
authors: GX
authors: Yang
authors: Ming
authors: M
authors: Chen
authors: Lian-Cheng
authors: LC
authors: Li
authors: You-Zhi
authors: YZ
authors: Ma
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D018525:Germination / Q000187:drug effects* / Q000235:genetics; D005875:Gibberellins / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D009154:Mutation / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology
chemical_list: C516466:AKINbeta1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D005875:Gibberellins; D014230:Triazoles; C483419:calcineurin B-like protein 1, Arabidopsis; C053370:paclobutrazol; D005947:Glucose