title: Electrophoretically deposited reduced graphene oxide platform for food toxin detection.
abstract: Reduced graphene oxide (RGO) due to its excellent electrochemical properties and large surface area, has recently aroused much interest for electrochemical biosensing application. Here, the chemically active RGO has been synthesized and deposited onto an indium tin oxide (ITO) coated glass substrate by the electrophoretic deposition technique. This novel platform has been utilized for covalent attachment of the monoclonal antibodies of aflatoxin B1 (anti-AFB1) for food toxin (AFB1) detection. The electron microscopy, X-ray diffraction, and UV-visible studies reveal successful synthesis of reduced graphene oxide while the XPS and FTIR studies suggest its carboxylic functionalized nature. The electrochemical sensing results of the anti-AFB1/RGO/ITO based immunoelectrode obtained as a function of aflatoxin concentration show high sensitivity (68 μA ng(-1) mL cm(-2)) and improved detection limit (0.12 ng mL(-1)). The association constant (ka) for antigen-antibody interaction obtained as 5 × 10(-4) ng mL(-1) indicates high affinity of antibodies toward the antigen (AFB1).
authors: Department of Science and Technology, Center on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory, Dr. K.S. Krishnan Road, New Delhi-110012, India.
authors: Saurabh
authors: S
authors: Srivastava
authors: Vinod
authors: V
authors: Kumar
authors: Md Azahar
authors: MA
authors: Ali
authors: Pratima R
authors: PR
authors: Solanki
authors: Anchal
authors: A
authors: Srivastava
authors: Gajjala
authors: G
authors: Sumana
authors: Preeti Suman
authors: PS
authors: Saxena
authors: Amish G
authors: AG
authors: Joshi
authors: B D
authors: BD
authors: Malhotra
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D004586:Electrophoresis; D004595:Electroplating; D005506:Food Contamination / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D014961:X-Ray Diffraction
chemical_list: D000911:Antibodies, Monoclonal; D010087:Oxides; D006108:Graphite; D016604:Aflatoxin B1