title: WormFarm: a quantitative control and measurement device toward automated Caenorhabditis elegans aging analysis.
abstract: Caenorhabditis elegans is a leading model organism for studying the basic mechanisms of aging. Progress has been limited, however, by the lack of an automated system for quantitative analysis of longevity and mean lifespan. To address this barrier, we developed 'WormFarm', an integrated microfluidic device for culturing nematodes. Cohorts of 30-50 animals are maintained throughout their lifespan in each of eight separate chambers on a single WormFarm polydimethylsiloxane chip. Design features allow for automated removal of progeny and efficient control of environmental conditions. In addition, we have developed computational algorithms for automated analysis of video footage to quantitate survival and other phenotypes, such as body size and motility. As proof-of-principle, we show here that WormFarm successfully recapitulates survival data obtained from a standard plate-based assay for both RNAi-mediated and dietary-induced changes in lifespan. Further, using a fluorescent reporter in conjunction with WormFarm, we report an age-associated decrease in fluorescent intensity of GFP in transgenic worms expressing GFP tagged with a mitochondrial import signal under the control of the myo-3 promoter. This marker may therefore serve as a useful biomarker of biological age and aging rate.
authors: CAS Key Laboratory for Computational Biology, CAS-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, China.
authors: Bo
authors: B
authors: Xian
authors: Jie
authors: J
authors: Shen
authors: Weiyang
authors: W
authors: Chen
authors: Na
authors: N
authors: Sun
authors: Nan
authors: N
authors: Qiao
authors: Dongqing
authors: D
authors: Jiang
authors: Tao
authors: T
authors: Yu
authors: Yongfan
authors: Y
authors: Men
authors: Zhijun
authors: Z
authors: Han
authors: Yuhong
authors: Y
authors: Pang
authors: Matt
authors: M
authors: Kaeberlein
authors: Yanyi
authors: Y
authors: Huang
authors: Jing-Dong J
authors: JD
authors: Han
mesh_terms: D000375:Aging / Q000235:genetics; D000465:Algorithms; D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers; D049628:Body Size; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D004129:Dimethylpolysiloxanes; D049452:Green Fluorescent Proteins / Q000235:genetics; D008124:Locomotion; D008136:Longevity / Q000235:genetics / Q000502:physiology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic; D034622:RNA Interference
chemical_list: D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; D004129:Dimethylpolysiloxanes; D049452:Green Fluorescent Proteins; C013830:baysilon; D018995:Myosin Heavy Chains