title: Improving antioxidant status in aged mice by 50% ethanol extract from red bean fermented by Bacillus subtilis.
abstract: BACKGROUND
  The purpose of this study was to investigate the effects of 50% ethanol extracts from red bean non-fermented (RBE) and fermented by Bacillus subtilis (RBNE) on the antioxidant status of aged ICR mouse.
  
  
  RESULTS
  Compared to 2-month-old ICR mouse, the plasma total antioxidant status (TAS) in 12-month-old ICR mouse decreased about 57%, while malondialdehyde (MDA) levels in the liver and brain of 12-month-old ICR mouse increased 56% and 30%, respectively. Orally administration of RBE or RBNE could completely recover the changes of MDA and plasma TAS levels due to the aging process. Vitamin E contents declined 88% in the liver and 74% in the brain of aged ICR mouse. At a level of 0.3 or 0.6 g kg(-1) body weight, RBNE raised vitamin E content in the liver and brain; however, RBE showed no significant influence. All antioxidant enzymes activities in the liver and brain of aged ICR mouse decreased compared to those activities in 2-month-old ICR mouse. RBNE could significantly enhance the superoxide dismutase activity in the brain of aged ICR mouse.
  
  
  CONCLUSIONS
  Oral administration of RBE or RBNE could improve antioxidant status in aged ICR mouse. Fermentation by Bacillus subtilis could enhance the antioxidant properties of red bean.
authors: Department of Food and Nutrition, Providence University, Shalu, Taichung City, Taiwan, Republic of China.
authors: Su-Tze
authors: ST
authors: Chou
authors: Yun-Chin
authors: YC
authors: Chung
authors: Hsin-Yi
authors: HY
authors: Peng
authors: Cheng-Kuang
authors: CK
authors: Hsu
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001412:Bacillus subtilis; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D007887:Fabaceae / Q000382:microbiology*; D005285:Fermentation; D005516:Food Microbiology; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D028321:Plant Preparations / Q000494:pharmacology; D012639:Seeds / Q000382:microbiology; D013482:Superoxide Dismutase / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D028321:Plant Preparations; D014810:Vitamin E; D008315:Malondialdehyde; D013482:Superoxide Dismutase