title: Evaluation of selenite effects on selenoproteins and cytokinome in human hepatoma cell lines.
abstract: The need to explore new alternative therapeutic strategies and chemoprevention methods for hepatocellular carcinoma is growing significantly. Selenium is a trace element that plays a critical role in physiological processes, and is used in cancer chemoprevention. The aim of this work was to test in vitro the effect of sodium selenite on the human hepatoma cell lines, HepG2 and Huh7, to assess its effect on the expression of GPX1, SELK and SELENBP1 and also to evaluate its action on inflammation determinants such as cytokines. Our results show that: (i) the increase observed for the GPX1 and SELK expression is correlated with an increase in the sodium selenite concentration, also evidencing an inverse association between the levels of these two proteins and SELENBP1; (ii) the selenium concentrations evaluated in protein extracts increase in proportional way with the selenite concentrations used in the treatment, suggesting that other selenoproteins can also be modulated and should be evaluated in further studies, and (iii) some cytokines, VEGF and three pro-inflammatory cytokines, i.e., IL-6, IL-8, and IL-17, decreased with an increasing selenite concentration. Finally, interactomic studies show that GPX1 and SELK, and the four pro-inflammatory cytokines are functionally correlated evidencing a putative anti-inflammatory role for the selenite.
authors: Cancer Research Center, "Pascale Foundation" National Cancer Institute, Mercogliano (AV) 83013, Italy.
authors: Fabiola
authors: F
authors: Rusolo
authors: Biagio
authors: B
authors: Pucci
authors: Giovanni
authors: G
authors: Colonna
authors: Francesca
authors: F
authors: Capone
authors: Eliana
authors: E
authors: Guerriero
authors: Maria Rita
authors: MR
authors: Milone
authors: Melissa
authors: M
authors: Nazzaro
authors: Maria Grazia
authors: MG
authors: Volpe
authors: Gianni
authors: G
authors: Di Bernardo
authors: Giuseppe
authors: G
authors: Castello
authors: Susan
authors: S
authors: Costantini
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D051137:Selenium-Binding Proteins / Q000378:metabolism; D051140:Selenoproteins / Q000378:metabolism*; D018038:Sodium Selenite / Q000494:pharmacology*; D000094765:Glutathione Peroxidase GPX1
chemical_list: D016207:Cytokines; C495175:SELENBP1 protein, human; D051137:Selenium-Binding Proteins; D051140:Selenoproteins; D005979:Glutathione Peroxidase; D018038:Sodium Selenite; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human