title: Allogeneic mesenchymal stem cell therapy for bisphosphonate-related jaw osteonecrosis in Swine.
abstract: Bisphosphonates (BPs), which are used to treat a variety of clinical disorders, have the side effect of jawbone necrosis. Currently, there is no reliable treatment for BP-related osteonecrosis of the jaw (BRONJ) due to a lack of understanding of its pathogenesis. To investigate the pathogenesis of BRONJ and observe the treatment effect of bone marrow mesenchymal stem cell (BMMSC) transplantation, we established a preclinical animal model of BRONJ in miniature pigs (minipigs). After treatment with zoledronic acid, the clinical and radiographic manifestations of BRONJ could be observed in minipigs after first premolar extraction. The biological and immunological properties of BMMSCs were impaired in the BP-treated minipigs. Moreover, the ratio of Foxp3-positive regulatory T-cells (Tregs) in peripheral blood decreased, and interleukin (IL)-17 increased in the serum of BP-treated minipigs. After allogeneic BMMSC transplantation via intravenous infusion, mucosal healing and bone reconstruction were observed; IL-17 levels were reduced; and Tregs were elevated. In summary, we established a clinically relevant BRONJ model in minipigs and tested a promising allogeneic BMMSC-based therapy, which may have potential clinical applications for treating BRONJ.
authors: Molecular Laboratory for Gene Therapy & Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
authors: Yunsheng
authors: Y
authors: Li
authors: Junji
authors: J
authors: Xu
authors: Lisha
authors: L
authors: Mao
authors: Yi
authors: Y
authors: Liu
authors: Runtao
authors: R
authors: Gao
authors: Zongmei
authors: Z
authors: Zheng
authors: Wanjun
authors: W
authors: Chen
authors: Anh
authors: A
authors: Le
authors: Songtao
authors: S
authors: Shi
authors: Songlin
authors: S
authors: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D059266:Bisphosphonate-Associated Osteonecrosis of the Jaw / Q000000981:diagnostic imaging / Q000473:pathology* / Q000628:therapy*; D004164:Diphosphonates; D004195:Disease Models, Animal; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D007093:Imidazoles; D020381:Interleukin-17 / Q000097:blood; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation; D011859:Radiography; D013552:Swine; D013556:Swine, Miniature; D050378:T-Lymphocytes, Regulatory / Q000473:pathology*; D014184:Transplantation, Homologous; D000077211:Zoledronic Acid
chemical_list: D015415:Biomarkers; D004164:Diphosphonates; D051858:Forkhead Transcription Factors; D007093:Imidazoles; D020381:Interleukin-17; D000077211:Zoledronic Acid