title: Ethanol and dietary unsaturated fat (corn oil/linoleic acid enriched) cause intestinal inflammation and impaired intestinal barrier defense in mice chronically fed alcohol.
abstract: Alcohol and dietary fat both play an important role in alcohol-mediated multi-organ pathology, including gut and liver. In the present study we hypothesized that the combination of alcohol and dietary unsaturated fat (USF) would result in intestinal inflammatory stress and mucus layer alterations, thus contributing to disruption of intestinal barrier integrity. C57BL/6N mice were fed Lieber-DeCarli liquid diets containing EtOH and enriched in USF (corn oil/linoleic acid) or SF (medium chain triglycerides: beef tallow) for 8 weeks. Intestinal histology, morphometry, markers of inflammation, as well as levels of mucus protective factors were evaluated. Alcohol and dietary USF triggered an intestinal pro-inflammatory response, characterized by increase in Tnf-α, MCP1, and MPO activity. Further, alcohol and dietary USF, but not SF, resulted in alterations of the intestinal mucus layer, characterized by decreased expression of Muc2 in the ileum. A strong correlation was observed between down-regulation of the antimicrobial factor Cramp and increased Tnf-α mRNA. Therefore, dietary unsaturated fat (corn oil/LA enriched) is a significant contributing factor to EtOH-mediated intestinal inflammatory response and mucus layer alterations in rodents.
authors: Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA. I0kirp01@louisville.edu
authors: Irina A
authors: IA
authors: Kirpich
authors: Wenke
authors: W
authors: Feng
authors: Yuhua
authors: Y
authors: Wang
authors: Yanlong
authors: Y
authors: Liu
authors: Juliane I
authors: JI
authors: Beier
authors: Gavin E
authors: GE
authors: Arteel
authors: K Cameron
authors: KC
authors: Falkner
authors: Shirish S
authors: SS
authors: Barve
authors: Craig J
authors: CJ
authors: McClain
mesh_terms: D000818:Animals; D003314:Corn Oil / Q000008:administration & dosage / Q000633:toxicity*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000633:toxicity; D004751:Enteritis / Q000139:chemically induced / Q000473:pathology*; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology*; D019787:Linoleic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D004042:Dietary Fats, Unsaturated; D000431:Ethanol; D003314:Corn Oil; D019787:Linoleic Acid