title: Potential endocrine disrupting effect of ochratoxin A on human placental 3β-hydroxysteroid dehydrogenase/isomerase in JEG-3 cells at levels relevant to human exposure.
abstract: Ochratoxin A (OTA) is a common foodborne mycotoxin. Besides its classical toxicities, it is also associated with the impairment of steroidogenesis in rats. It is hypothesized that OTA may act as an endocrine disruptor by intervening 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD). To address this hypothesis, human placental cells JEG-3 were used in vitro to examine the effects of short- and long-term OTA exposures on expression levels of 3β-HSD1 and progesterone secretion at 24-96h. Results showed that both cytotoxic and non-cytotoxic levels of OTA induced 3β-HSD1 mRNA expression by 281-378% at 72 and 96h. A significant induction (43-316%) of 3β-HSD1 protein expression was observed at 48, 72 and 96h, and the progesterone production with the involvement of 3β-HSD1 was significantly increased by 22-89% after 48-96h. This is the first study to demonstrate OTA up-regulates 3β-HSD1 expression in human placental cells, indicating the potential endocrine-disrupting property of OTA.
authors: School of Biological Sciences, Faculty of Science, Kadoorie Biological Sciences Building, The University of Hong Kong, Pokfulam, Hong Kong.
authors: Chit Shing Jackson
authors: CS
authors: Woo
authors: Murphy Lam Yim
authors: ML
authors: Wan
authors: Jorma
authors: J
authors: Ahokas
authors: Hani
authors: H
authors: El-Nezami
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D052244:Endocrine Disruptors / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D009793:Ochratoxins / Q000008:administration & dosage*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D009793:Ochratoxins; D012333:RNA, Messenger; C025589:ochratoxin A; D011374:Progesterone; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase