title: [Prediction and identification of B-cell linear epitopes of hepatitis B e antigen].
abstract: OBJECTIVE
  To predict and identify B-cell linear epitopes of hepatitis B e antigen (HBeAg).
  
  
  METHODS
  The B-cell linear epitopes of HBeAg were predicted using the software provided by NCBI Database and Immune Epitope Database (IEDB) and synthesized by a solid-phase method followed by conjugation with keyhole limpet hemocyanin (KLH). The KLH conjugates were used for immunization of New Zealand white rabbits, and the immune response of the rabbits was monitored by direct ELISA using a bovine serum albumin conjugate of the predicted epitopes. RESULTS Four new B-cell linear epitopes of HBeAg were identified, namely (1)MDIDPYKEFG(10), (37)LYREALESPEHCSP(50), (74)SNLEDPAS(81) and (127)RTPPAYRPPNAPIL(140). The rabbits immunized with the KLH conjugate showed an antibody titer over 1:512 000. The antisera of B-cell linear epitopes collected could specifically react with HBeAg as shown by ELISA.
  
  
  CONCLUSIONS
  Four B-cell linear epitopes of HBeAg have been confirmed using bioinformatics methods, which provides new evidence for further functional studies of HBeAg in hepatitis B.
authors: Department of Pathology, Second Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, China. yangjundr@yahoo.com.cn
authors: Jun
authors: J
authors: Yang
authors: Ni
authors: N
authors: Liu
authors: Ting
authors: T
authors: Zhang
authors: Shiping
authors: S
authors: Zhao
authors: Lei
authors: L
authors: Qiang
authors: Baoshan
authors: B
authors: Su
authors: Anjing
authors: A
authors: Kang
authors: Zongfang
authors: Z
authors: Li
mesh_terms: D000818:Animals; D019295:Computational Biology; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006513:Hepatitis B e Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D011817:Rabbits
chemical_list: D018985:Epitopes, B-Lymphocyte; D006513:Hepatitis B e Antigens