title: Expression analysis of LeNHX1 gene in mycorrhizal tomato under salt stress.
abstract: The plant growth, stem sap flow, Na(+) and Cl(-) content, and the expression of vacuolar Na(+)/H(+) antiporter gene (LeNHX1) in the leaves and roots of tomato under different NaCl stresses (0.5% and 1%) were studied to analyze the effect of arbuscular mycorrhizal fungi (AMF) on Na(+) and Cl(-) accumulation and ion exchange. The results showed that arbuscular mycorrhizal (AM) plant growth and stem sap flow increased and salt tolerance improved, whereas Na(+) and Cl(-) accumulated. Na(+) significantly decreased, and no significant decline was detected in Cl(-) content after AMF inoculation compared with the non-AM plants. The LeNHX1 gene expression was induced in the AM and non-AM plants by NaCl stress. However, AMF did not improve the LeNHX1 level, and low expression was observed in the AM tomato. Hence, the mechanism that reduced the Na(+) damage to tomato induced by AMF has little relation to LeNHX1, which can export Na(+) from the cytosol to the vacuole across the tonoplast.
authors: College of Horticulture, Sichuan Agricultural University, Yáan, Sichuan 625014, P. R. China. hzqun328@163.com
authors: Zhongqun
authors: Z
authors: He
authors: Zhi
authors: Z
authors: Huang
mesh_terms: D002713:Chlorine / Q000032:analysis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics* / Q000382:microbiology; D038821:Mycorrhizae / Q000254:growth & development*; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000382:microbiology; D012492:Salts / Q000633:toxicity; D012964:Sodium / Q000032:analysis; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012492:Salts; D017923:Sodium-Hydrogen Exchangers; D002713:Chlorine; D012964:Sodium