title: Evaluation of the humoral immune response and cross reactivity against Mycobacterium tuberculosis of mice immunized with liposomes containing glycolipids of Mycobacterium smegmatis.
abstract: Mycobacterium smegmatis (Ms) is a nonpathogenic mycobacteria of rapid growth, which shares many characteristics with Mycobacterium tuberculosis (MTB), the major causative agent of tuberculosis. MTB has several cell wall glycolipids in common with Ms, which play an important role in the pathogenesis of tuberculosis and the induction of a protective immune response against MTB infection in some animal models. In this study, the humoral immune response and cross reactivity against MTB, of liposomes containing a mixture of cell wall glycolipids of Ms and commercial lipids was evaluated, in order to study its possible use as a component of a vaccine candidate against tuberculosis. Liposomes containing total lipids extracted from Ms, distearoyl phosphatidyl choline and cholesterol were prepared by the dehydration-rehydration technique. Balb/c mice were immunized with the liposomes obtained and the antibody response and cross reactivity against MTB were tested by ELISA. Total lipids extract from Ms showed the presence of several polar glycolipids in common with MTB, such as phosphatidylinositol mannosides. Liposomes that contained glycolipids of Ms were capable of inducing a specific IgG antibody response that allowed the recognition of surface antigens of MTB. The results of this study demonstrated the presence of immunogenic glycolipids in Ms, which could be included to enhance the protective effects of subunit vaccine formulations against tuberculosis.
authors: Molecular Biology Department, Finlay Institute, La Lisa, La Habana, Cuba, P.O. Box 16017.
authors: Reinier
authors: R
authors: Borrero
authors: María de los A
authors: Mde L
authors: García
authors: Liem
authors: L
authors: Canet
authors: Caridad
authors: C
authors: Zayas
authors: Fátima
authors: F
authors: Reyes
authors: Jorge L
authors: JL
authors: Prieto
authors: Juan F
authors: JF
authors: Infante
authors: María E
authors: ME
authors: Lanio
authors: Ramlah
authors: R
authors: Kadir
authors: Yamilé
authors: Y
authors: López
authors: María E
authors: ME
authors: Sarmiento
authors: Mohd
authors: M
authors: Nor Norazmi
authors: Armando
authors: A
authors: Acosta
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D002784:Cholesterol; D003429:Cross Reactions; D006017:Glycolipids / Q000008:administration & dosage / Q000276:immunology*; D056724:Immunity, Humoral; D008081:Liposomes / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020102:Mycobacterium smegmatis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control; D032581:Tuberculosis Vaccines / Q000276:immunology*; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006017:Glycolipids; D008081:Liposomes; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D032581:Tuberculosis Vaccines; C034154:phosphatidylinositol mannoside; D002784:Cholesterol; C010942:1,2-distearoyllecithin