title: Simultaneous determination of flavonoids, isochlorogenic acids and triterpenoids in Ilex hainanensis Using high performance liquid chromatography coupled with diode array and evaporative light scattering detection.
abstract: A high performance liquid chromatography coupled with diode array and evaporative light scattering detection (HPLC-DAD-ELSD) method for simultaneous determination of eight major bioactive compounds including two flavonoids (rutin and eriodictyol-7-O-β-D-glucopyranoside), two isochlorogenic acids (isochlorogenic acid A and isochlorogenic acid C) and four triterpenoids (ilexhainanoside D, ilexsaponin A1, ilexgenin A and ursolic acid) in Ilex hainanensis has been developed for the first time. The 283 nm wavelength was chosen for determination of two flavonoids and two isochlorogenic acids. ELSD was applied to determine four triterpenoids. The analysis was performed on an Agilent Zorbax SB-C18 column (250 × 4.6 mm i.d., 5 µm) with gradient elution of 0.2% formic acid in water and acetonitrile. The method was validated for linearity, limit of detection, limit of quantification, precision, repeatability and accuracy. The proposed method has been successfully applied for simultaneous quantification of the analytes in four samples of Ilex hainanensis, which is helpful for quality control of this plant.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
authors: Bo
authors: B
authors: Peng
authors: Chun-Feng
authors: CF
authors: Qiao
authors: Jing
authors: J
authors: Zhao
authors: Wei-Hua
authors: WH
authors: Huang
authors: De-Jun
authors: DJ
authors: Hu
authors: Hua-Gang
authors: HG
authors: Liu
authors: Shao-Ping
authors: SP
authors: Li
mesh_terms: D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry*; D030017:Ilex / Q000737:chemistry*; D054023:Lasers, Semiconductor; D008027:Light; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D014315:Triterpenes / Q000032:analysis / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D014315:Triterpenes; C469310:isochlorogenic acid; D002726:Chlorogenic Acid