title: Silaffin peptides as a novel signal enhancer for gravimetric biosensors.
abstract: Application of biomimetic silica formation to gravimetric biosensors has been conducted for the first time. As a model system, silaffin peptides fused with green fluorescent protein (GFP) were immobilized on a gold quartz crystal resonator for quartz crystal microbalances using a self-assembled monolayer. When a solution of silicic acid was supplied, silica particles were successfully deposited on the Au surface, resulting in a significant change in resonance frequency (i.e., signal enhancement) with the silaffin-GFP. However, frequency was not altered when bare GFP was used as a control. The novel peptide enhancer is advantageous because it can be readily and quantitatively conjugated with sensing proteins using recombinant DNA technology. As a proof of concept, this study shows that the silaffin domains can be employed as a novel and efficient biomolecular signal enhancer for gravimetric biosensors.
authors: Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul 139-701, Republic of Korea.
authors: Dong Hyun
authors: DH
authors: Nam
authors: Jeong-O
authors: JO
authors: Lee
authors: Byoung-In
authors: BI
authors: Sang
authors: Keehoon
authors: K
authors: Won
authors: Yong Hwan
authors: YH
authors: Kim
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D006046:Gold; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D055767:Immobilized Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D011791:Quartz; D058525:Quartz Crystal Microbalance Techniques; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D012824:Silicic Acid; D012822:Silicon Dioxide
chemical_list: D055767:Immobilized Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D012824:Silicic Acid; D049452:Green Fluorescent Proteins; D011791:Quartz; D006046:Gold; D012822:Silicon Dioxide