title: Lab in a tube: ultrasensitive detection of microRNAs at the single-cell level and in breast cancer patients using quadratic isothermal amplification.
abstract: Through rational design of a functional molecular probe with high sequence specificity that takes advantage of sensitive isothermal amplification with simple operation, we developed a one-pot hairpin-mediated quadratic enzymatic amplification strategy for microRNA (miRNA) detection. Our method exhibits ultrahigh sensitivity toward miR-21 with detection limits of 10 fM at 37 °C and 1 aM at 4 °C, which corresponds to nine strands of miR-21 in a 15 μL sample, and it is capable of distinguishing among miRNA family members. More importantly, the proposed approach is also sensitive and selective when applied to crude extractions from MCF-7 and PC3 cell lines and even patient tissues from intraductal carcinoma and invasive ductal carcinoma of the breast.
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Ruixue
authors: R
authors: Duan
authors: Xiaolei
authors: X
authors: Zuo
authors: Shutao
authors: S
authors: Wang
authors: Xiyun
authors: X
authors: Quan
authors: Dongliang
authors: D
authors: Chen
authors: Zhifei
authors: Z
authors: Chen
authors: Lei
authors: L
authors: Jiang
authors: Chunhai
authors: C
authors: Fan
authors: Fan
authors: F
authors: Xia
mesh_terms: D001940:Breast / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs