title: Phosphatidic acid metabolism in rat liver cell nuclei.
abstract: The aim of the present research was to analyze the pathways for phosphatidic acid metabolism in purified nuclei from liver. Lipid phosphate phosphatase, diacylglycerol lipase, monoacylglycerol lipase and PA-phospholipase type A activities were detected. The presence of lysophosphatidic acid significantly reduced DAG production while sphingosine 1-phoshate and ceramide 1-phosphate reduced MAG formation from PA. Using different enzymatic modulators (detergents and ions) an increase in the PA metabolism by phospholipase type A was observed. Our findings evidence an active PA metabolism in purified liver nuclei which generates important lipid second messengers, and which could thus be involved in nuclear processes such as gene transcription.
authors: Instituto de Investigaciones Bioquímicas de Bahía Blanca, Universidad Nacional del Sur and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bahía Blanca, Argentina.
authors: Virginia L
authors: VL
authors: Gaveglio
authors: Susana J
authors: SJ
authors: Pasquaré
authors: Norma M
authors: NM
authors: Giusto
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002518:Ceramides / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D015151:Immunoblotting; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008246:Lysophospholipids / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D008994:Monoacylglycerol Lipases / Q000378:metabolism; D050178:Monoglycerides / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D010711:Phosphatidate Phosphatase / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013110:Sphingosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D002518:Ceramides; D004075:Diglycerides; D008246:Lysophospholipids; D050178:Monoglycerides; D010712:Phosphatidic Acids; C065576:ceramide 1-phosphate; C060506:sphingosine 1-phosphate; D017830:Octoxynol; D008994:Monoacylglycerol Lipases; D010741:Phospholipases A; D008071:Lipoprotein Lipase; C112948:lipid phosphate phosphatase; D010711:Phosphatidate Phosphatase; D008274:Magnesium; D013110:Sphingosine; C032881:lysophosphatidic acid; D002118:Calcium