title: Comparison of global responses to mild deficiency and excess copper levels in Arabidopsis seedlings.
abstract: Copper is an essential micronutrient in higher plants, but it is toxic in excess. The fine adjustments required to fit copper nutritional demands for optimal growth are illustrated by the diverse, severe symptoms resulting from copper deficiency and excess. Here, a differential transcriptomic analysis was done between Arabidopsis thaliana plants suffering from mild copper deficiency and those with a slight copper excess. The effects on the genes encoding cuproproteins or copper homeostasis factors were included in a CuAt database, which was organised to collect additional information and connections to other databases. The categories overrepresented under copper deficiency and copper excess conditions are discussed. Different members of the categories overrepresented under copper deficiency conditions were both dependent and independent of the general copper deficiency transcriptional regulator SPL7. The putative regulatory elements in the promoter of the copper deficiency overrepresented genes, particularly of the iron superoxide dismutase gene FSD1, were also analysed. A 65 base pair promoter fragment, with at least three GTAC sequences, was found to be not only characteristic of them all, but was responsible for most of the FSD1 copper-dependent regulations. Moreover, a new molecular marker for the slight excess copper nutritional status is proposed. Taken together, these data further contribute to characterise copper nutritional responses in higher plants.
authors: Departament de Bioquímica i Biologia Molecular, Universitat de València, Avda. Dr Moliner 50, ES-46100 Burjassot, Valencia, Spain. nuria.andres@uv.es
authors: Nuria
authors: N
authors: Andrés-Colás
authors: Ana
authors: A
authors: Perea-García
authors: Sonia
authors: S
authors: Mayo de Andrés
authors: Antoni
authors: A
authors: Garcia-Molina
authors: Eavan
authors: E
authors: Dorcey
authors: Susana
authors: S
authors: Rodríguez-Navarro
authors: Miguel A
authors: MA
authors: Pérez-Amador
authors: Sergi
authors: S
authors: Puig
authors: Lola
authors: L
authors: Peñarrubia
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006706:Homeostasis; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C483288:SPL7 protein, Arabidopsis; D014157:Transcription Factors; D003300:Copper; D013482:Superoxide Dismutase