title: Cross-neutralizing activity of human anti-V3 monoclonal antibodies derived from non-B clade HIV-1 infected individuals.
abstract: One approach to the development of an HIV vaccine is to design a protein template which can present gp120 epitopes inducing cross-neutralizing antibodies. To select a V3 sequence for immunogen design, we compared the neutralizing activities of 18 anti-V3 monoclonal antibodies (mAbs) derived from Cameroonian and Indian individuals infected with clade AG and C, respectively. It was found that V3 mAbs from the Cameroonian patients were significantly more cross-neutralizing than those from India. Interestingly, superior neutralizing activity of Cameroonian mAbs was also observed among the nine VH5-51/VL lambda genes encoding V3 mAbs which mediate a similar mode of recognition. This correlated with higher relative binding affinity to a variety of gp120s and increased mutation rates in V3 mAbs from Cameroon. These results suggest that clade C V3 is probably weakly immunogenic and that the V3 sequence of CRF02_AG viruses can serve as a plausible template for vaccine immunogen design.
authors: Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
authors: Raiees
authors: R
authors: Andrabi
authors: Constance
authors: C
authors: Williams
authors: Xiao-Hong
authors: XH
authors: Wang
authors: Liuzhe
authors: L
authors: Li
authors: Alok K
authors: AK
authors: Choudhary
authors: Naveet
authors: N
authors: Wig
authors: Ashutosh
authors: A
authors: Biswas
authors: Kalpana
authors: K
authors: Luthra
authors: Arthur
authors: A
authors: Nadas
authors: Michael S
authors: MS
authors: Seaman
authors: Phillipe
authors: P
authors: Nyambi
authors: Susan
authors: S
authors: Zolla-Pazner
authors: Miroslaw K
authors: MK
authors: Gorny
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D002163:Cameroon; D003429:Cross Reactions; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007194:India; D009500:Neutralization Tests
chemical_list: D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C514793:gp120 protein, Human immunodeficiency virus 1