title: Snail inhibits Notch1 intracellular domain mediated transcriptional activation via competing with MAML1.
abstract: Notch1 intracellular domain (NICD) is the transcription factor which controls cell fate and differentiation in embryonic and tumor cells. Snail has a critical role which increases invasion and metastasis of cancer cell as a transcription factor and epigenetic regulator. Recently, we discovered NICD induced Snail degradation by direct binding interaction with Snail. In this experiment, we found that Snail suppressed transcriptional activity of the protein complex formed with NICD and RBPJk in nucleus. Moreover, Snail decreased transcription of NICD target genes via competing with MAML1, co-activator, in NICD complex. In conclusion, Snail inhibited NICD-mediated transcriptional activation of target genes by physical interaction with NICD.
authors: Department of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors: Hyeon Seop
authors: HS
authors: Kim
authors: Heewon
authors: H
authors: Jeong
authors: Seung-Oe
authors: SO
authors: Lim
authors: Guhung
authors: G
authors: Jung
mesh_terms: D001667:Binding, Competitive; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000235:genetics / Q000378:metabolism; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051881:Receptor, Notch1 / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; C430872:MAML1 protein, human; D046912:Multiprotein Complexes; C497761:NOTCH1 protein, human; C497570:RBPJ protein, human; D051881:Receptor, Notch1; D011994:Recombinant Proteins; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors