title: Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.
abstract: This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
authors: LRGP, UPR CNRS 3349, Lorraine University, Plateforme SVS, 13 rue du bois de la Champelle, 54500 Vandœuvre-lès-Nancy, France.
authors: Claudia
authors: C
authors: Nioi
authors: Romain
authors: R
authors: Kapel
authors: Emmanuel
authors: E
authors: Rondags
authors: Ivan
authors: I
authors: Marc
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D029741:Brassica rapa / Q000737:chemistry*; D002850:Chromatography, Gel; D005670:Fusarium / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts