title: In vivo evaluation of the interaction between titanium dioxide nanoparticle and rat liver DNA.
abstract: Titanium dioxide nanoparticles (TiO2 NPs) are massively produced and widely used in daily life, which may pose potential risk to human health via uncharacterized interaction between DNAs. This research aims to examine the interaction between DNA and three types of TiO2 NPs of different sizes and crystallines. The interaction between TiO2 NPs and liver DNA molecules obtained from Sprague-Dawley rats was systematically evaluated in vivo using atomic force microscopy, transmission electron microscopy, various spectroscopic techniques and gel electrophoresis. We found that TiO2 NPs (diameter <25 nm and <100 nm) in anatase crystalline can covalently interact with liver DNA by either inserting itself in between DNA base pairs or binding to DNA nucleotide via P-O-Ti-O bond. Such interaction may not be NP size-dependent but may be crystalline phase-dependent, because such interaction did not occur in rutile crystal phase, in which the DNA damage was potentially caused by reactive oxygen species.
authors: Institute of Biophysics, Second Military Medical University, Shanghai, People's Republic of China.
authors: Chan
authors: C
authors: Jin
authors: Ying
authors: Y
authors: Tang
authors: X Yan
authors: XY
authors: Fan
authors: X Ting
authors: XT
authors: Ye
authors: X Lin
authors: XL
authors: Li
authors: Kai
authors: K
authors: Tang
authors: Y Fang
authors: YF
authors: Zhang
authors: A Guo
authors: AG
authors: Li
authors: Y Ji
authors: YJ
authors: Yang
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006651:Histocytochemistry; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D009690:Nucleic Acid Conformation; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D014025:Titanium / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*
chemical_list: C009495:titanium dioxide; D004247:DNA; D014025:Titanium