title: Chemical ligation of oligodeoxynucleotides by X-irradiation and its application to regulation of G-quadruplex formation.
abstract: We demonstrated radiolytic ligation of oligodeoxynucleotides (ODNs) possessing disulfide bond and its application to regulation of DNA quadruplex formation. G-rich hexamer ODNs had poor ability to form quadruplex, while X-irradiation of the ODNs induced interstrand exchange reaction at disulfide bond to form ligated 12 mer ODNs, leading to the ready formation of quadruplex due to the entropic effect. Since complexation of the ligated ODNs with hemin in the presence of K(+) showed strong soret band absorption and also catalyzed the H(2)O(2)-mediated oxidation of luminol, it appears that the quadruplex formed from ligated ODNs showed a function similar to native DNA quadruplex.
authors: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. tanabeka@scl.kyoto-u.ac.jp
authors: Kazuhito
authors: K
authors: Tanabe
authors: Taiga
authors: T
authors: Mizoe
authors: Masaaki
authors: M
authors: Sugiura
authors: Kana
authors: K
authors: Okada
authors: Sei-ichi
authors: S
authors: Nishimoto
mesh_terms: D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D014965:X-Rays
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA