title: Enzyme responsive hyaluronic acid nanocapsules containing polyhexanide and their exposure to bacteria to prevent infection.
abstract: Antibacterial nanodevices could bring coatings of plastic materials and wound dressings a big step forward if the release of the antibacterial agents could be triggered by the presence of the bacteria themselves. Here, we show that novel hyaluronic acid (HA)-based nanocapsules containing the antimicrobial agent polyhexanide are specifically cleaved in the presence of hyaluronidase, a factor of pathogenicity and invasion for bacteria like Staphylococcus aureus and Escherichia coli. This resulted in an efficient killing of the pathogenic bacteria by the antimicrobial agent. The formation of different polymeric nanocapsules was achieved through a polyaddition reaction in inverse miniemulsion. After the synthesis, the nanocapsules were transferred to an aqueous medium and investigated in terms of size, size distribution, functionality, and morphology using dynamic light scattering, zeta potential measurements and scanning electron microscopy. The enzyme triggered release of a model dye and the antimicrobial polyhexanide was monitored using fluorescence and UV spectroscopy. The stability of the nanocapsules in several biological media was tested and the interaction of nanocapsules with human serum protein was studied using isothermal titration calorimetry. The antibacterial effectiveness is demonstrated by determination of the antibacterial activity and determination of the minimal bactericidal concentration (MBC).
authors: Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors: Grit
authors: G
authors: Baier
authors: Alex
authors: A
authors: Cavallaro
authors: Krasimir
authors: K
authors: Vasilev
authors: Volker
authors: V
authors: Mailänder
authors: Anna
authors: A
authors: Musyanovych
authors: Katharina
authors: K
authors: Landfester
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001645:Biguanides / Q000494:pharmacology*; D004337:Drug Carriers; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000517:prevention & control; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D053769:Nanocapsules / Q000737:chemistry* / Q000627:therapeutic use; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001645:Biguanides; D004337:Drug Carriers; D053769:Nanocapsules; C031233:polihexanide; D006820:Hyaluronic Acid