title: Radioprotective effect of Haberlea rhodopensis (Friv.) leaf extract on gamma-radiation-induced DNA damage, lipid peroxidation and antioxidant levels in rabbit blood.
abstract: Different concentrations of H. rhodopensis total extract (HRE; 0.03, 0.06 and 0.12 g/kg body weight) were injected im, into rabbits 2 h before collecting the blood samples. The whole blood samples were exposed in vitro to 2.0 Gy 60Co gamma-radiation. The radiation-induced changes were estimated by using the chromosome aberration test (CA) and cytokinesis blocked micronucleus assay (CBMN) in peripheral lymphocytes, and by determining the malondialdehyde levels (MDA) in blood plasma and the superoxide dismutase (SOD) and catalase (CAT) activity in erythrocytes. Radiation significantly increased the chromosome aberration and micronuclei frequencies as well as MDA levels and decreased the antioxidant enzyme activity. On the other hand, the HRE pretreatment significantly decreased the CA, MN frequencies and MDA levels and increased the SOD and CAT activity in a concentration dependent manner. The most effective was the highest concentration of HRE (0.12 g/kg body weight). The results suggest that HRE as a natural product with a nantioxidant capacity could play a modulatory role against the cellular damage induced by gamma-irradiation. The possible mechanism involved in the radioprotective potential of HRE is discussed.
authors: Department of Genetics, Animal Breeding & Reproduction, Trakia University, Stara Zagora 6000, Bulgaria. sgeorg@af.uni-sz.bg
authors: Svetlana
authors: S
authors: Georgieva
authors: Borislav
authors: B
authors: Popov
authors: Georgi
authors: G
authors: Bonev
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002869:Chromosome Aberrations; D003470:Culture Media; D004249:DNA Damage / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology; D005609:Free Radicals; D005720:Gamma Rays; D015227:Lipid Peroxidation; D019684:Magnoliopsida / Q000378:metabolism*; D008297:Male; D015162:Micronucleus Tests / Q000379:methods; D008954:Models, Biological; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D011832:Radiation Injuries / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003470:Culture Media; D005609:Free Radicals; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D002374:Catalase; D013482:Superoxide Dismutase