title: Determination of total oxidative stress and total antioxidant capacity before and after the treatment of iron-deficiency anemia.
abstract: BACKGROUND
  Oxidative stress is an imbalance between the reactive oxygen species and antioxidant system. In this study, total oxidative stress (TOS) and total antioxidant capacity (TAC) were investigated with a new and practical method in childhood iron-deficiency anemia.
  
  
  METHODS
  During the study period 80 children between 6 and 60 months were enrolled; 40 children (study group) had iron-deficiency anemia, and 40 children (control group) were healthy. Complete blood count, serum iron, iron-binding capacity, ferritin levels, TOS, and TAC were evaluated. Children diagnosed iron-deficiency anemia were treated with oral ferric iron. After 2 months of the treatment, blood tests of the study groups were repeated to check the challenge.
  
  
  RESULTS
  TAC was similar between both groups (1.55 ± 0.26 in control group 1.53 ± 0.19 mmol Trolox Eq./l). Additionally, TOS was significantly higher in iron-deficiency anemia group before treatment with iron (24.3 ± 18.5, in controls groups 14.4 ± 7.1 mmol Trolox Eq./l). We have shown that TAC did not change (before treatment 1.55 ± 0.26, after treatment 1.54 ± 0.26 mmol Trolox Eq./l) although TOS decreased significantly after the treatment of iron-deficiency anemia (before treatment 24.3 ± 18.5, after treatment 12.4 ± 6.9 mmol Trolox Eq./l). We did not find any correlation between hemoglobin, serum iron, iron-binding capacity, ferritin levels, and TOS or TAC among iron-deficiency anemia patients.
  
  
  CONCLUSIONS
  As a result of this study, oxidative stress increases in children with iron-deficiency anemia and this increase can be returned to normal levels by treatment.
authors: Faculty of Medicine, Department of Pediatrics, Fatih University, Ankara, Turkey. haliseakca@gmail.com
authors: Halise
authors: H
authors: Akça
authors: Aziz
authors: A
authors: Polat
authors: Cemile
authors: C
authors: Koca
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000097:blood / Q000188:drug therapy* / Q000453:epidemiology / Q000378:metabolism*; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D016022:Case-Control Studies; D002675:Child, Preschool; D002839:Chromans / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D007223:Infant; D007501:Iron / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018384:Oxidative Stress; D018709:Statistics, Nonparametric
chemical_list: D000975:Antioxidants; D002839:Chromans; D007501:Iron; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid