title: Genetic analysis of Porphyromonas gingivalis (fimA), Aggregatibacter actinomycetemcomitans, and red complex in coronary plaque.
abstract: OBJECTIVE
  The objective of the present study was to detect the presence of Porphyromonas gingivalis (fimA), Aggregatibacter actinomycetemcomitans, and red complex in the coronary plaque of patients with coronary artery disease.
  
  
  METHODS
  The study population consisted of 51 patients with chronic periodontitis undergoing coronary artery bypass grafting. DNA was extracted from subgingival and coronary atherosclerotic plaque samples. Polymerase chain reaction was used to amplify the part of 16S rRNA gene to detect the presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis (fimA), Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola.
  
  
  RESULTS
  Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, Porphyromonas gingivalis (fimA), and Treponema denticola were detected in 0%, 31.4%, 45.1%, 39.2%, and 51% of the atherosclerotic plaque samples, respectively. In both subgingival and coronary atherosclerotic plaque samples, Tannerella forsythia was detected in 19.6%, Porphyromonas gingivalis in 39.2%, Porphyromonas gingivalis (fimA) in 33.3%, and Treponema denticola in 35.3% of the samples.
  
  
  CONCLUSIONS
  The study confirmed the detection of red complex bacteria in coronary plaque samples. However Aggregatibacter actinomycetemcomitans could not be detected in these samples.
authors: Department of Periodontology, Meenakshi Ammal Dental College, Meenakshi Academy of Higher Education and Research, Chennai, India.
authors: Jaideep
authors: J
authors: Mahendra
authors: Little
authors: L
authors: Mahendra
authors: John
authors: J
authors: Felix
authors: Georgios E
authors: GE
authors: Romanos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification / Q000235:genetics*; D001439:Bacteroides / Q000145:classification / Q000235:genetics; D055113:Chronic Periodontitis / Q000150:complications / Q000382:microbiology; D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000382:microbiology* / Q000601:surgery; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D003774:Dental Plaque Index; D005260:Female; D036781:Fimbriae Proteins / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018933:Pili, Sex / Q000145:classification / Q000235:genetics*; D058226:Plaque, Atherosclerotic / Q000382:microbiology* / Q000601:surgery; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000145:classification / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D048373:Treponema denticola / Q000145:classification / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C063055:fimbrillin; D036781:Fimbriae Proteins
keywords: atherosclerosis; chronic periodontitis; coronary artery disease; inflammation; polymerase chain reaction