title: [Study on ITS sequences of Aconitum vilmorinianum and its medicinal adulterant].
abstract: OBJECTIVE
  To analyze and compare the ITS sequences of Aconitum vilmorinianum and its medicinal adulterant Aconitum austroyunnanense.
  
  
  METHODS
  Total genomic DNA were extracted from sample materials by improved CTAB method, ITS sequences of samples were amplified using PCR systems, directly sequenced and analyzed using software DNAStar, ClustalX1.81 and MEGA 4.0.
  
  
  RESULTS
  299 consistent sites, 19 variable sites and 13 informative sites were found in ITS1 sequences, 162 consistent sites, 2 variable sites and 1 informative sites were found in 5.8S sequences, 217 consistent sites, 3 variable sites and 1 informative site were found in ITS2 sequences. Base transition and transversion was not found only in 5.8S sequences, 2 sites transition and 1 site transversion were found in ITS1 sequences, only 1 site transversion was found in ITS2 sequences comparting the ITS sequences data matrix. By analyzing the ITS sequences data matrix from 2 population of Aconitum vilmorinianum and 3 population of Aconitum austroyunnanense, we found a stable informative site at the 596th base in ITS2 sequences, in all the samples of Aconitum vilmorinianum the base was C, and in all the samples of Aconitum austroyunnanense the base was A.
  
  
  CONCLUSIONS
  Aconitum vilmorinianum and Aconitum austroyunnanense can be identified by their characters of ITS sequences, and the variable sites in ITS1 sequences are more than in ITS2 sequences.
authors: Yunnan institute of materia medica, Kunming 650111, China. xiaonan1242@126.com
authors: Xiao-nan
authors: XN
authors: Zhang
authors: Chun-hua
authors: CH
authors: Du
authors: De-huan
authors: DH
authors: Fu
authors: Li
authors: L
authors: Gao
authors: Pei-jun
authors: PJ
authors: Zhou
authors: Li
authors: L
authors: Wang
mesh_terms: D031841:Aconitum / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics*; D004340:Drug Contamination; D014644:Genetic Variation; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011786:Quality Control; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer