title: Endonuclease IV discriminates mismatches next to the apurinic/apyrimidinic site in DNA strands: constructing DNA sensing platforms with extremely high selectivity.
abstract: A unique capability of Endonuclease IV in discrimination of mismatches neighboring a natural abasic site in DNA strands has been demonstrated, which enables genotyping of SNPs with high discrimination factors and differentiation of as low as 0.1-0.01% of target DNA strands from a large background of single-base different interfering strands.
authors: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors: Xianjin
authors: X
authors: Xiao
authors: Yang
authors: Y
authors: Liu
authors: Meiping
authors: M
authors: Zhao
mesh_terms: D001080:Apurinic Acid / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism; D043223:Deoxyribonuclease IV (Phage T4-Induced) / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D007700:Kinetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011119:Polynucleotides / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D011119:Polynucleotides; C001790:apyrimidinic acid; D001080:Apurinic Acid; D004247:DNA; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase