title: [Evaluation of the immobilization of one HIV-2 gp36 peptide in nitrocellulose membrane].
abstract: BACKGROUND
  antigen immobilization in solid supports is used for the development of several immunoassays. One of the first technologies developed was the protein adsorption by direct application to nitrocellulose.
  
  
  OBJECTIVE
  to standardize the immobilization of a synthetic peptide of the HIV-2 transmembrane protein gp36 to nitrocellulose support for diagnostic purposes and to evaluate the performance parameters in a group of serum samples with recognized interesting reactivity.
  
  
  METHODS
  the peptide was freely immobilized, conjugated to bovine serum albumin (BSA) and to keyhole limpet hemocyanin (KLH) as carrier proteins. Immobilization parameters were analyzed and then, the optimal immobilization alternative was determined. Using the chosen variant, the diagnostic sensitivity and specificity against reference panels of the AIDS Research Laboratory were evaluated. Analytical specificity was evaluated with reactive samples to HIV-1 and HTLV-1.
  
  
  RESULTS
  the analysis of the immobilized peptide variants to nitrocellulose membranes showed that the gp36 peptide-BSA was the one that succeeded in setting the greatest differentiation between positive and negative samples. There were observed 100 % sensitivity, 95.2 % diagnostic specificity and 100 % analytical specificity.
  
  
  CONCLUSIONS
  the gp36-BSA peptide immobilized on nitrocellulose membranes showed efficacy for the serological diagnosis of HIV-2, which will allow considering this peptide for diagnostic uses in systems with nitrocellulose -based solid phase.
authors: Laboratorio de Investigaciones del SIDA (LISIDA), La Habana, Cuba. feloany@infomed.sld.cu
authors: Dervel Felipe
authors: DF
authors: Díaz Herrera
authors: Otto
authors: O
authors: Cruz Sui
authors: Lucy
authors: L
authors: Montano Tamayo
authors: Eladio
authors: E
authors: Silva Cabrera
mesh_terms: D003101:Collodion; D008567:Membranes, Artificial; D054299:env Gene Products, Human Immunodeficiency Virus / Q000032:analysis*
chemical_list: D008567:Membranes, Artificial; D054299:env Gene Products, Human Immunodeficiency Virus; C059763:env protein gp36, Human immunodeficiency virus; D003101:Collodion