title: CYP2E1*5B, CYP2E1*6, CYP2E1*7B, CYP2E1*2, and CYP2E1*3 allele frequencies in iranian populations.
abstract: BACKGROUND
  CYP2E1 encodes an enzyme which is mainly involved in bioactivation of potential carcinogens such as N-nitrosamines. Polymorphisms in the gene have been reported to be associated with cancer. The aim of this study was to evaluate genotype distributions and allele frequencies of five CYP2E1 polymorphisms in Iran.
  
  
  METHODS
  Two hundred healthy individuals of an Iranian population from the southwest were included in this study. PCR-restriction fragment length polymorphism and Tetra-ARMS PCR methods were applied for CYP2E1 genotyping.
  
  
  RESULTS
  The allele frequencies for *5B, *6, *7B, *2, and *3 were calculated to be 1.5%, 16%, 28.5%, 0%, and 2.75% respectively. Results of this study showed that no significant differences in genotype and allele frequencies of five single nucleotide polymorphisms with respect to the gender and tribes. The chi-square test showed that the genotype frequencies of CYP2E1*5B were similar to Caucasians, but the distribution of CYP2E1*6 genotypes was similar to Asians. The frequencies of CYP2E1*2 (0%) and CYP2E1*3 (2.75%) alleles were within the range for Caucasians and Orientals. In the case of CYP2E1*7B, the data werelimited. Accordingly, the results were only compared with Europeans and the comparison showed significant differences.
  
  
  CONCLUSIONS
  In conclusion, ethnic and geographic differences may explain discrepancies in the prevalence of CYP2E1 polymorphisms.
authors: Department of Genetics, Faculty of Mathematics, Shaheed Chamran University, Ahvaz, Iran. amjalali@hotmail.com
authors: Ghazaleh Mohammadzadeh
authors: GM
authors: Shahriary
authors: Hamid
authors: H
authors: Galehdari
authors: Amir
authors: A
authors: Jalali
authors: Fatemeh
authors: F
authors: Zanganeh
authors: Seyed Mohammad Reza
authors: SM
authors: Alavi
authors: Mohammad Reza
authors: MR
authors: Aghanoori
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007223:Infant; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D055815:Young Adult
chemical_list: D019392:Cytochrome P-450 CYP2E1