title: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45.
abstract: OBJECTIVE
  Side population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many kinds of cell lines and tissues have demonstrated the presence of SP cells, including several gastric cancer cell lines. This study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line MKN-45.
  
  
  METHODS
  We used fluorescence activated cell sorting (FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45 (cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP cells.
  
  
  RESULTS
  This study found that the SP cells had higher clone formation efficiency than major population (MP) cells. Five stemness-related gene expression profiles, including OCT-4, SOX-2, NANOG, CD44, and adenosine triphosphate (ATP)-binding cassette transporters gene ABCG2, were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). Western blot was used to show the difference of protein expression between SP and MP cells. Both results show that there was significantly higher protein expression in SP cells than in MP cells. When inoculated into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, SP cells show higher tumorigenesis tendency than MP cells.
  
  
  CONCLUSIONS
  These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells.
authors: Department of General Surgery, General Hospital of the Air Force, PLA, Beijing, China.
authors: Hai-hong
authors: HH
authors: Zhang
authors: Ai-zhen
authors: AZ
authors: Cai
authors: Xue-ming
authors: XM
authors: Wei
authors: Li
authors: L
authors: Ding
authors: Feng-zhi
authors: FZ
authors: Li
authors: Ai-ming
authors: AM
authors: Zheng
authors: Da-jiang
authors: DJ
authors: Dai
authors: Rong-rong
authors: RR
authors: Huang
authors: Hou-jun
authors: HJ
authors: Cao
authors: Hai-yang
authors: HY
authors: Zhou
authors: Jian-mei
authors: JM
authors: Wang
authors: Xue-jing
authors: XJ
authors: Wang
authors: Wei
authors: W
authors: Shi
authors: Heng
authors: H
authors: Zhu
authors: Xiao-ying
authors: XY
authors: Yuan
authors: Lin
authors: L
authors: Chen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000145:classification / Q000378:metabolism* / Q000473:pathology*; D013274:Stomach Neoplasms / Q000145:classification / Q000378:metabolism* / Q000473:pathology*
chemical_list: D009363:Neoplasm Proteins