title: Neuroprotective effect of alkyl hydroxytyrosyl ethers in rat brain slices subjected to a hypoxia-reoxygenation model.
abstract: The aim of the present study was to investigate the antioxidant and possible neuroprotective and antioxidant effects of five alkyl hydroxytyrosyl (HT) ethers (ethyl, butyl, hexyl, octyl and dodecyl) in rat brain slices. None of the compounds modified lipid peroxidation or glutathione concentrations (GSH) in oxygenated samples. The effects of oxidative stress were investigated with ferrous salts to induce lipid peroxidation and diethylmaleate (DEM) to reduce GSH. All compounds inhibited lipid peroxidation with an inhibitory concentration 50% (IC(50)) one tenth that of HT. These compounds, especially the butyl derivative, prevented GSH depletion after incubation with DEM. We also explored the neuroprotective effect of these compounds in an experimental model of hypoxia-reoxygenation in rat brain slices. All compounds showed neuroprotective and antioxidant effects. Our results established a relationship between these effects and the length of the carbon chain (maximum effect in the range of C4-C8).
authors: Laboratorio de Investigaciones Antitrombóticas e Isquemia Tisular (LIAIT), Department of Pharmacology and Therapeutics, School of Medicine, University of Málaga, 29071 Málaga, Spain.
authors: A
authors: A
authors: Guerrero
authors: J P
authors: JP
authors: De la Cruz
authors: J
authors: J
authors: Muñoz-Marín
authors: J A
authors: JA
authors: López-Villodres
authors: A
authors: A
authors: Madrona
authors: J L
authors: JL
authors: Espartero
authors: J A
authors: JA
authors: González-Correa
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004987:Ethers / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D000860:Hypoxia / Q000188:drug therapy / Q000378:metabolism*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008954:Models, Biological; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004987:Ethers; D018696:Neuroprotective Agents; C005975:3,4-dihydroxyphenylethanol; D005978:Glutathione; D010626:Phenylethyl Alcohol; D010100:Oxygen