title: Assessment of the aggregation propensity of the β -amyloid peptide during the synthesis and when free in solution.
abstract: This work developed an alternative approach targeting the evaluation of the aggregation propensity of the (1-42) β-amyloid peptide (Alzheimer's disease) and some segments, either attached to a polymer during their synthesis or when free in solution. The solvation behavior of peptide-resins was gauged by measuring the swelling of beads in a microscope and the degree of chain motion through EPR spectra of previously labeled resins with an amino acid-type probe. In terms of comparative solvent dissociation power towards aggregated structures, the findings revealed greater values of peptide-resin swelling, peptide chain mobility and solubility when in strong electron donor dimethylsulfoxide than in strong electron acceptor trifluoroethanol. Otherwise, the weakest chain-chain disruption power was verified for acetonitrile, an internally neutral solvent in terms of Lewis acid/base properties. In complement, fluorescence and light scattering experiments depicted that the 15-35 region plays an essential role in the amyloid peptide fibril formation capacity.
authors: Department of Physiological Sciences, Faculdade de Ciências Médicas da Santa Casa de Sao Paulo, SP, Brazil.
authors: Luciana
authors: L
authors: Malavolta
authors: Marcelo R S
authors: MR
authors: Pinto
authors: Clóvis R
authors: CR
authors: Nakaie
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D002942:Circular Dichroism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D017433:Protein Structure, Secondary; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D011108:Polymers; D012996:Solutions; D012997:Solvents; C075222:amyloid beta-protein (1-42)