title: Heat-shock-mediated conditional regulation of hedgehog/gli signaling in zebrafish.
abstract: BACKGROUND
  Hedgehog (Hh) signaling is required for embryogenesis and continues to play key roles postembryonically in many tissues, influencing growth, stem cell proliferation, and tumorigenesis. Systems for conditional regulation of Hh signaling facilitate the study of these postembryonic Hh functions.
  
  
  RESULTS
  We used the hsp70l promoter to generated three heat-shock-inducible transgenic lines that activate Hh signaling and one line that represses Hh signaling. Heat-shock activation of these transgenes appropriately recapitulates early embryonic loss or gain of Hh function phenotypes. Hh signaling remains activated 24 hr after heat shock in the Tg(hsp70l:shha-EGFP) and Tg(hsp70l:dnPKA-BGFP) lines, while a single heat shock of the Tg(hsp70l:gli1-EGFP) or Tg(hsp70l:gli2aDR-EGFP) lines results in a 6- to 12-hr pulse of Hh signal activation or inactivation, respectively. Using both in situ hybridization and quantitative polymerase chain reaction, we show that these lines can be used to manipulate Hh signaling through larval and juvenile stages. A ptch2 promoter element was used to generate new reporter lines that allow clear visualization of Hh responding cells throughout the life cycle, including graded Hh responses in the embryonic central nervous system.
  
  
  CONCLUSIONS
  These zebrafish transgenic lines provide important new experimental tools to study the embryonic and postembryonic roles of Hh signaling.
authors: Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors: Meng-Chieh
authors: MC
authors: Shen
authors: A Tuba
authors: AT
authors: Ozacar
authors: Marcey
authors: M
authors: Osgood
authors: Crina
authors: C
authors: Boeras
authors: Jochebed
authors: J
authors: Pink
authors: Jeanne
authors: J
authors: Thomas
authors: Jhumku D
authors: JD
authors: Kohtz
authors: Rolf
authors: R
authors: Karlstrom
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics / Q000502:physiology*; D053823:Hedgehog Proteins / Q000235:genetics* / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism; D000071676:Zinc Finger Protein GLI1
chemical_list: D018840:HSP70 Heat-Shock Proteins; D053823:Hedgehog Proteins; D015513:Oncogene Proteins; D015534:Trans-Activators; D029961:Zebrafish Proteins; D000071676:Zinc Finger Protein GLI1; D049452:Green Fluorescent Proteins