title: Rapid and Facile Microwave-Assisted Surface Chemistry for Functionalized Microarray Slides.
abstract: We describe a rapid and facile method for surface functionalization and ligand patterning of glass slides based on microwave-assisted synthesis and a microarraying robot. Our optimized reaction enables surface modification 42-times faster than conventional techniques and includes a carboxylated self-assembled monolayer, polyethylene glycol linkers of varying length, and stable amide bonds to small molecule, peptide, or protein ligands to be screened for binding to living cells. We also describe customized slide racks that permit functionalization of 100 slides at a time to produce a cost-efficient, highly reproducible batch process. Ligand spots can be positioned on the glass slides precisely using a microarraying robot, and spot size adjusted for any desired application. Using this system, we demonstrate live cell binding to a variety of ligands and optimize PEG linker length. Taken together, the technology we describe should enable high-throughput screening of disease-specific ligands that bind to living cells.
authors: Robotic Chemistry Group, Center for Molecular Imaging, Beth Israel Deaconess Medical Center, Boston, MA 02215 ; Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215.
authors: Jeong Heon
authors: JH
authors: Lee
authors: Hoon
authors: H
authors: Hyun
authors: Conor J
authors: CJ
authors: Cross
authors: Maged
authors: M
authors: Henary
authors: Khaled A
authors: KA
authors: Nasr
authors: Rafiou
authors: R
authors: Oketokoun
authors: Hak Soo
authors: HS
authors: Choi
authors: John V
authors: JV
authors: Frangioni
keywords: Cell-Based Assays; Drug Discovery; High-Throughput Screening; Microarrays; Microwave-Assisted Synthesis; Surface Chemistry