title: [Construction of special reporter to detect DNA methylation regulatory activity in FCER1G gene promoter through patch-methylation].
abstract: OBJECTIVE
  To construct a special luciferase reporter to detect DNA methylation regulatory activity in FCER1G gene promoter regulatory element.
  
  
  METHODS
  We constructed special full and mock methylated FCER1G gene promoter regulatory luciferase reporters by patch-methylation, and detected DNA methylation regulatory activity by comparing the luciferase activity of full-methylated luciferase reporters with mock-methylated reporters.
  
  
  RESULTS
  We successfully constructed the full and mock methylated FCER1G gene promoter regulatory luciferase reporters. The ratio of luciferase activity between the full methylated and the mock methylated was (0.36±0.07):1 (P<0.001).
  
  
  CONCLUSIONS
  FCER1G promoter activity is methylation-sensitive and is regulated by DNA methylation.
authors: Department of Dermatology, Central South University, Changsha, China.
authors: Yunsheng
authors: Y
authors: Liang
authors: Ming
authors: M
authors: Zhao
authors: Gongping
authors: G
authors: Liang
authors: Heng
authors: H
authors: Yin
authors: Qianjin
authors: Q
authors: Lu
mesh_terms: D001483:Base Sequence; D019175:DNA Methylation; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D017455:Receptors, IgE / Q000235:genetics* / Q000378:metabolism
chemical_list: C543372:FcepsilonRI gamma-chain, human; D017455:Receptors, IgE; D008156:Luciferases