title: 8-Isoprostane: a lipid peroxidation product in gingival crevicular fluid in healthy, gingivitis and chronic periodontitis subjects.
abstract: OBJECTIVE
  The idea that reactive oxygen species (ROS) are associated with the pathogenesis of inflammatory periodontal diseases and have a role (direct or indirect) in tissue damage has become a major area of research over the last decade. The purpose of this study is to determine, presence of 8-isoprostane in gingival crevicular fluid (GCF) in healthy, gingivitis and chronic periodontitis (CP) subjects and to find an association, if any between GCF 8-isoprostane levels and clinical periodontal parameters.
  
  
  METHODS
  78 subjects (40 males and 38 females) were selected based on their clinical parameters into three groups: Group 1 (26 healthy), Group 2 (26 gingivitis subjects) and Group 3 (26 CP subjects). GCF 8-isoprostane levels were estimated by ELISA.
  
  
  RESULTS
  The 8-isoprostane concentration in GCF was highest in subjects with chronic periodontitis as compared to gingivitis and healthy subjects and a significant association was observed between GCF 8-isoprostane levels and all periodontal parameters.
  
  
  CONCLUSIONS
  There was increase in 8-isoprostane levels in GCF as the disease process progressed from health to gingivitis and chronic periodontitis, suggesting a role for increased oxidative stress in CP.
authors: Department of Periodontics, Government Dental College and Research Institute, Bangalore 560002, India. periodonticsgdcri@gmail.com
authors: A R
authors: AR
authors: Pradeep
authors: Nishanth S
authors: NS
authors: Rao
authors: Pavan
authors: P
authors: Bajaj
authors: Esha
authors: E
authors: Agarwal
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000378:metabolism*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010512:Periodontal Index; D018709:Statistics, Nonparametric; D014662:Vasoconstrictor Agents / Q000032:analysis / Q000378:metabolism*
chemical_list: D014662:Vasoconstrictor Agents; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost