title: Caspase-3 activation and increased procollagen type I in irradiated hearts.
abstract: The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.
authors: Universidade Federal Fluminense, Instituto de Biologia, Departamento de Biologia Geral, Niterói, RJ, Brasil. samararossatti@gmail.com
authors: Samara C
authors: SC
authors: Ferreira-Machado
authors: Camila
authors: C
authors: Salata
authors: Nazareth N
authors: NN
authors: Rocha
authors: Alexandre F S
authors: AF
authors: Corrêa
authors: Suzana
authors: S
authors: Côrte-Real
authors: Antônio A F
authors: AA
authors: Peregrino
authors: Vera M A
authors: VM
authors: de Campos
authors: Cherley B V
authors: CB
authors: Andrade
authors: Mario
authors: M
authors: Bernardo-Filho
authors: Januário B
authors: JB
authors: Cabral-Neto
authors: Carlos E
authors: CE
authors: Dealmeida
mesh_terms: D000818:Animals; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D004452:Echocardiography; D004789:Enzyme Activation / Q000528:radiation effects; D005260:Female; D006321:Heart / Q000528:radiation effects*; D046529:Microscopy, Electron, Transmission; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D053773:Transforming Growth Factor beta1; D053148:Caspase 3