title: Glycoprotein C plays a role in the adsorption of duck enteritis virus to chicken embryo fibroblasts cells and in infectivity.
abstract: Unlike glycoprotein C (gC) of many mammalian herpes viruses, gC of some avian herpes viruses does not play a principle role in the binding of virus to heparin sulfate proteoglycans on the cell surface. The roles of duck enteritis virus (DEV) gC on viral attachment remained unclear. In this study, we showed that gC expressed in vitro could bind to chicken embryo fibroblasts (CEF) cells and inhibit the adsorption of duck enteritis virus (DEV) onto CEF cells effectively and antiserum directed against gC inhibited the infection of DEV. Furthermore, it was confirmed that gC protein expressed in baculovirus system did not bind to heparin-Sepharose beads and virus binding to cells were independent of heparin sulfate proteoglycans on the surface of cells. Therefore, gC contributes to adsorption and infection of DEV.
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Yong
authors: Y
authors: Hu
authors: Xiaokun
authors: X
authors: Liu
authors: Zhong
authors: Z
authors: Zou
authors: Meilin
authors: M
authors: Jin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D004372:Ducks; D005347:Fibroblasts / Q000821:virology*; D025922:Mardivirus / Q000472:pathogenicity / Q000502:physiology*; D015678:Viral Structural Proteins / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: D015678:Viral Structural Proteins