title: Impact of cardiac stem cell sheet transplantation on myocardial infarction.
abstract: OBJECTIVE
  Myocardial infarction (MI) remains a major cause of mortality because of the limited regenerative capacity of the myocardium. Transplantation of somatic tissue-derived cells into the heart has been shown to enhance the endogenous healing process, but the magnitude of its therapeutic effects is dependent upon the cell-source or cell-delivery method. We investigated the therapeutic effects of C-Kit positive cardiac cell (CSC) cell-sheet transplantation therapy in a rat model of MI.
  
  
  RESULTS
  CSCs of human origin were sorted and cultured to generate scaffold-free CSC cell-sheets. One-layered or 3-layered cell-sheets were transplanted into nude rats 1 h after left coronary artery ligation. We observed a significant increase in the left ventricular ejection fraction and a significant decrease in left ventricular systolic dimension at 2 and 4 weeks in the 3-layer group, but not in the 1-layer or sham groups. Consistently, there was less accumulation of interstitial fibrosis in the 3-layer group than in the 1-layer or sham groups. Moreover, capillary density was significantly greater in the 3-layer group than in the 1-layer or sham groups.
  
  
  CONCLUSIONS
  The 3-layered cell-sheet improved cardiac function associated with angiogenic and anti-fibrotic effects. Thus, CSC is a promising cell-source to use with the cell-sheet method for the treatment of cardiac failure, as long as a sufficient number of cells are delivered.
authors: Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita 565-0871, Japan.
authors: Sfoug
authors: S
authors: Alshammary
authors: Satsuki
authors: S
authors: Fukushima
authors: Shigeru
authors: S
authors: Miyagawa
authors: Takenori
authors: T
authors: Matsuda
authors: Hiroyuki
authors: H
authors: Nishi
authors: Atsuhiro
authors: A
authors: Saito
authors: Sokichi
authors: S
authors: Kamata
authors: Takayuki
authors: T
authors: Asahara
authors: Yoshiki
authors: Y
authors: Sawa
mesh_terms: D000368:Aged; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005355:Fibrosis; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009203:Myocardial Infarction / Q000473:pathology* / Q000628:therapy*; D009206:Myocardium / Q000166:cytology* / Q000473:pathology; D018919:Neovascularization, Physiologic; D051381:Rats; D033581:Stem Cell Transplantation / Q000379:methods*