title: A Porphyra columbina hydrolysate upregulates IL-10 production in rat macrophages and lymphocytes through an NF-κB, and p38 and JNK dependent mechanism.
abstract: The marine environment represents a relatively untapped source of functional ingredients. Here we characterise a hydrolysate obtained from Phorphyra columbina (PcRH) and its effects on primary splenocytes, macrophages and T lymphocytes in vitro. Our product had a high degree of hydrolysis, due to the use of a mixture of endo-peptidase and exo-peptidase, and was enriched in Asp, Ala and Glu. PcRH had mitogenic effects on rat splenic lymphocytes. IL-10 secretion was enhanced by PcRH in splenocytes (235%), macrophages (150%) and in lymphocytes (472%), while the production of TNFα and other proinflammatory cytokines by macrophages was inhibited (15-75%), especially under lipopolysaccharide stimulation. The effect of the hydrolysate on IL-10 was evoked by JNK, p38 MAPK and NF-κB dependent pathways in T lymphocytes. We conclude that PcRH has immunomodulatory effects on macrophages and lymphocytes, activating NF-κB and MAPK dependent pathways, and predominantly inducing IL-10 production.
authors: Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, 1° de Mayo 3250, (3000) Santa Fe, Argentina.
authors: Raúl E
authors: RE
authors: Cian
authors: Rocío
authors: R
authors: López-Posadas
authors: Silvina R
authors: SR
authors: Drago
authors: Fermín
authors: F
authors: Sánchez de Medina
authors: Olga
authors: O
authors: Martínez-Augustin
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D044664:Porphyra / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000461:Rhodophyta / Q000737:chemistry; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D011492:Protein Hydrolysates; D016753:Interleukin-10; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases