title: Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets.
abstract: The house dust mite (HDM) Dermatophagoides pteronyssinus is one of most important allergen sources and a major elicitor of allergic asthma. We screened a D. pteronyssinus expression cDNA library with IgE Abs from HDM allergic patients. A cDNA coding for a new major allergen was isolated, which showed sequence homology to peritrophins, which contain chitin-binding domains and are part of the peritrophic matrix lining the gut of arthropods. The mature Der p 23 allergen was expressed in Escherichia coli as an 8-kDa protein without its hydrophobic leader sequence and purified to homogeneity. It reacted with IgE Abs from 74% of D. pteronyssinus allergic patients (n = 347) at levels comparable to the two major HDM allergens, Der p 1 and Der p 2. Thus, Der p 23 represents a new major D. pteronyssinus allergen. Furthermore, rDer p 23 exhibited high allergenic activity as demonstrated by upregulation of CD203c expression on basophils from D. pteronyssinus allergic patients. Immunogold electron microscopy localized the allergen in the peritrophic matrix lining the midgut of D. pteronyssinus as well as on the surface of the fecal pellets. Thus, we identified a new major D. pteronyssinus allergen as peritrophin-like protein. The high allergenic activity of Der p 23 and its frequent recognition as respiratory allergen may be explained by the fact that it becomes airborne and respirable through its association with mite feces. Der p 23 may be an essential component for diagnosis and specific immunotherapy of HDM allergy.
authors: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.
authors: Margit
authors: M
authors: Weghofer
authors: Monika
authors: M
authors: Grote
authors: Yvonne
authors: Y
authors: Resch
authors: Anne
authors: A
authors: Casset
authors: Michael
authors: M
authors: Kneidinger
authors: Jolanta
authors: J
authors: Kopec
authors: Wayne R
authors: WR
authors: Thomas
authors: Enrique
authors: E
authors: Fernández-Caldas
authors: Michael
authors: M
authors: Kabesch
authors: Rosetta
authors: R
authors: Ferrara
authors: Adriano
authors: A
authors: Mari
authors: Ashok
authors: A
authors: Purohit
authors: Gabrielle
authors: G
authors: Pauli
authors: Friedrich
authors: F
authors: Horak
authors: Walter
authors: W
authors: Keller
authors: Peter
authors: P
authors: Valent
authors: Rudolf
authors: R
authors: Valenta
authors: Susanne
authors: S
authors: Vrtala
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D001491:Basophils / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D040002:Dermatophagoides pteronyssinus / Q000235:genetics / Q000276:immunology*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D007073:Immunoglobulin E