title: [Prevalence of dyslipidemia in middle-aged adults with NOS3 gene polymorphism and low cardiorespiratory fitness].
abstract: OBJECTIVE
  To evaluate the influence of the interaction between endothelial nitric oxide synthase gene (NOS3) polymorphisms at positions -786T>C, Glu298Asp and intron 4b/a, and cardiorespiratory fitness on plasma nitrite/nitrate levels, blood pressure, lipid profile, and prevalence of cardiometabolic disorders.
  
  
  METHODS
  Ninety-two volunteers were genotyped for NOS3 polymorphisms at positions (-786T>C and Glu298Asp) and (intron 4b/a) and divided according to the genotype: non-polymorphic (NP) and polymorphic (P). After that, they were subdivided according to the cardiorespiratory fitness associated with genotype: high (HNP and HP) and low (LNP and LP).
  
  
  RESULTS
  The subjects with polymorphism for the interactions at positions Glu298Asp + intron 4b/a, and Glu298Asp+-786T>C showed the highest values in total cholesterol, as well as dyslipidemia.
  
  
  CONCLUSIONS
  Our findings show that NOS3 gene polymorphisms at positions -786T>C, Glu298Asp, and intron 4b/a exert negative effects on the lipid profile compared with those who do not carry polymorphisms.
authors: Laboratório de Fisiologia Cardiovascular e Atividade Física, Instituto de Biociências, Universidade Estadual Paulista (Unesp), Rio Claro, SP, Brasil.
authors: Pamella A
authors: PA
authors: Malagrino
authors: Carlos H G
authors: CH
authors: Sponton
authors: Rodrigo D
authors: RD
authors: Esposti
authors: Carla F
authors: CF
authors: Franco-Penteado
authors: Romulo A
authors: RA
authors: Fernandes
authors: Marcos André C
authors: MA
authors: Bezerra
authors: Dulcinéia M
authors: DM
authors: Albuquerque
authors: Cynara M
authors: CM
authors: Rodovalho
authors: Maurício
authors: M
authors: Bacci
authors: Angelina
authors: A
authors: Zanesco
mesh_terms: D002319:Cardiovascular System / Q000503:physiopathology; D002784:Cholesterol / Q000097:blood; D050171:Dyslipidemias / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D004812:Epidemiologic Methods; D005260:Female; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D009573:Nitrites / Q000097:blood; D010101:Oxygen Consumption / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012137:Respiratory System / Q000503:physiopathology
chemical_list: D009566:Nitrates; D009573:Nitrites; D018698:Glutamic Acid; D002784:Cholesterol; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III