title: Mouse model of chronic and binge ethanol feeding (the NIAAA model).
abstract: Chronic alcohol consumption is a leading cause of chronic liver disease worldwide, leading to cirrhosis and hepatocellular carcinoma. Currently, the most widely used model for alcoholic liver injury is ad libitum feeding with the Lieber-DeCarli liquid diet containing ethanol for 4-6 weeks; however, this model, without the addition of a secondary insult, only induces mild steatosis, slight elevation of serum alanine transaminase (ALT) and little or no inflammation. Here we describe a simple mouse model of alcoholic liver injury by chronic ethanol feeding (10-d ad libitum oral feeding with the Lieber-DeCarli ethanol liquid diet) plus a single binge ethanol feeding. This protocol for chronic-plus-single-binge ethanol feeding synergistically induces liver injury, inflammation and fatty liver, which mimics acute-on-chronic alcoholic liver injury in patients. This feeding protocol can also be extended to chronic feeding for longer periods of time up to 8 weeks plus single or multiple binges. Chronic-binge ethanol feeding leads to high blood alcohol levels; thus, this simple model will be very useful for the study of alcoholic liver disease (ALD) and of other organs damaged by alcohol consumption.
authors: Laboratory of Liver Diseases, National Institute on Alcohol Abuse and Alcoholism NIAAA, National Institutes of Health NIH, Bethesda, Maryland, USA.
authors: Adeline
authors: A
authors: Bertola
authors: Stephanie
authors: S
authors: Mathews
authors: Sung Hwan
authors: SH
authors: Ki
authors: Hua
authors: H
authors: Wang
authors: Bin
authors: B
authors: Gao
mesh_terms: D000437:Alcoholism / Q000473:pathology*; D000818:Animals; D063425:Binge Drinking / Q000473:pathology*; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology; D008108:Liver Diseases, Alcoholic / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D054563:National Institute on Alcohol Abuse and Alcoholism (U.S.); D014481:United States
chemical_list: D000431:Ethanol