title: Shotgun proteomic analysis of two Bartonella quintana strains.
abstract: This study reports the first extensive shotgun analysis of the Bartonella quintana proteome. Proteins extracted from two B. quintana strains, Oklahoma and JK31, were analyzed in triplicate analyses by a bottom-up approach consisting of tryptic digestion in SDS-containing buffer, strong cation-exchange StageTip fractionation, and nano-LC-MS/MS analysis. By setting spectral false discovery rate below 0.5%, 548 unique proteins were identified overall, of which 409 protein identifications were shared between the two strains. The data set, which achieves the highest proteome coverage for B. quintana to date, could be exploited for the quantitative analysis of a selected subset of target proteins.
authors: Department of Health Sciences, Institute of Microbiology, "Magna Graecia" University of Catanzaro, Catanzaro, Italy.
authors: Alessandra
authors: A
authors: Fabietti
authors: Marco
authors: M
authors: Gaspari
authors: Shibu
authors: S
authors: Krishnan
authors: Angela
authors: A
authors: Quirino
authors: Maria Carla
authors: MC
authors: Liberto
authors: Giovanni
authors: G
authors: Cuda
authors: Alfredo
authors: A
authors: Focà
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis*; D018413:Bartonella quintana / Q000378:metabolism*; D002412:Cations; D005591:Chemical Fractionation; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002412:Cations; D012967:Sodium Dodecyl Sulfate; D010447:Peptide Hydrolases