title: A network model of the molecular organization of chromatin in Drosophila.
abstract: Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associated with chromatin, which all display specific genomic binding patterns. Bayesian network modeling of the binding profiles of these and 70 known chromatin components yields a detailed blueprint of the in vivo chromatin protein network. We demonstrate functional compartmentalization of this network, and predict functions for most of the previously unknown chromatin proteins, including roles in DNA replication and repair, and gene activation and repression.
authors: Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands.
authors: Joke G
authors: JG
authors: van Bemmel
authors: Guillaume J
authors: GJ
authors: Filion
authors: Arantxa
authors: A
authors: Rosado
authors: Wendy
authors: W
authors: Talhout
authors: Marcel
authors: M
authors: de Haas
authors: Tibor
authors: T
authors: van Welsem
authors: Fred
authors: F
authors: van Leeuwen
authors: Bas
authors: B
authors: van Steensel
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D001665:Binding Sites; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D059006:Chromosomes, Insect / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D008954:Models, Biological; D058977:Molecular Sequence Annotation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D025341:Principal Component Analysis; D011485:Protein Binding; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D011499:Protein Processing, Post-Translational
chemical_list: D002843:Chromatin; D029721:Drosophila Proteins; D009687:Nuclear Proteins