title: Genomics-based high-resolution mapping of the BaMMV/BaYMV resistance gene rym11 in barley (Hordeum vulgare L.).
abstract: Soil-borne barley yellow mosaic virus disease, caused by different strains of Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV), is one of the most important diseases of winter barley (Hordeum vulgare L.) in Europe and East Asia. The recessive resistance gene rym11 located in the centromeric region of chromosome 4HL is effective against all so far known strains of BaMMV and BaYMV in Germany. In order to isolate this gene, a high-resolution mapping population (10,204 meiotic events) has been constructed. F2 plants were screened with co-dominant flanking markers and segmental recombinant inbred lines (RILs) were tested for resistance to BaMMV under growth chamber and field conditions. Tightly linked markers were developed by exploiting (1) publicly available barley EST sequences, (2) employing barley synteny to rice, Brachypodium distachyon and sorghum and (3) using next-generation sequencing data of barley. Using this approach, the genetic interval was efficiently narrowed down from the initial 10.72 % recombination to 0.074 % recombination. A marker co-segregating with rym11 was developed providing the basis for gene isolation and efficient marker-assisted selection.
authors: Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany.
authors: Thomas
authors: T
authors: Lüpken
authors: Nils
authors: N
authors: Stein
authors: Dragan
authors: D
authors: Perovic
authors: Antje
authors: A
authors: Habekuss
authors: Ilona
authors: I
authors: Krämer
authors: Urs
authors: U
authors: Hähnel
authors: Burkhard
authors: B
authors: Steuernagel
authors: Uwe
authors: U
authors: Scholz
authors: Rounan
authors: R
authors: Zhou
authors: Ruvini
authors: R
authors: Ariyadasa
authors: Stefan
authors: S
authors: Taudien
authors: Matthias
authors: M
authors: Platzer
authors: Mihaela
authors: M
authors: Martis
authors: Klaus
authors: K
authors: Mayer
authors: Wolfgang
authors: W
authors: Friedt
authors: Frank
authors: F
authors: Ordon
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D023281:Genomics; D001467:Hordeum / Q000235:genetics* / Q000276:immunology / Q000821:virology; D007113:Immunity, Innate / Q000235:genetics*; D009029:Mosaic Viruses / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D026801:Synteny
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers