title: Scanning of novel cancer/testis proteins by human testis proteomic analysis.
abstract: The testes are where spermatogenesis, the sperm-generating process that is unique to men, occurs. Importantly, human spermatogenesis and tumorigenesis share key similarities. Until now, only a few proteins in the human testis have been identified due to limitations of available technology. In this paper, using an advanced proteomics platform, we have identified 7346 unique proteins within the human testis with a high degree of confidence. Immunohistochemistry data from the Human Protein Atlas database show over 90% (1833/2020) of identified proteins can be detected in the human testis using specific antibodies. To make the data widely available to the scientific community, an online Human Testis Proteome Database (HTPD, http://reprod.njmu.edu.cn/htpd/) was built. Many of the identified human testicular proteins are associated with human infertility, especially human testicular predominantly expressed proteins. We characterized six novel cancer/testis genes (TMPRSS12, TPPP2, PRSS55, DMRT1, PIWIL1, HEMGN), which map to cancer-associated genetic variants positions, in both the cancer and testis tissues using genome-wide analyses. Our results provide a molecular connection between spermatogenesis and tumorigenesis and broaden the range of cancer antigen choice available for immunotherapy.
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors: Mingxi
authors: M
authors: Liu
authors: Zhibin
authors: Z
authors: Hu
authors: Lin
authors: L
authors: Qi
authors: Jing
authors: J
authors: Wang
authors: Tao
authors: T
authors: Zhou
authors: Yueshuai
authors: Y
authors: Guo
authors: Yan
authors: Y
authors: Zeng
authors: Bo
authors: B
authors: Zheng
authors: Yibo
authors: Y
authors: Wu
authors: Pan
authors: P
authors: Zhang
authors: Xin
authors: X
authors: Chen
authors: Wenjiao
authors: W
authors: Tu
authors: Ting
authors: T
authors: Zhang
authors: Quan
authors: Q
authors: Zhou
authors: Min
authors: M
authors: Jiang
authors: Xuejiang
authors: X
authors: Guo
authors: Zuomin
authors: Z
authors: Zhou
authors: Jiahao
authors: J
authors: Sha
mesh_terms: D000328:Adult; D030562:Databases, Protein; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013737:Testis / Q000378:metabolism*; D046888:Tissue Array Analysis
chemical_list: D009363:Neoplasm Proteins; D010455:Peptides; D020543:Proteome