title: Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood kraft pulp.
abstract: In this paper we describe the effect of enzyme treatments on the production of polymeric xylan, oligosaccharides and hemicellulose lean pulp by alkaline extraction of bleached hardwood kraft pulp. Enzyme treatments were carried out before one or in between two subsequent alkaline extractions by purified Trichoderma reesei xylanase and endoglucanase II (Cel 5a) as well as by a commercial monocomponent endoglucanase (FibreCareR). Without enzyme pre-treatment 61% and 7% of the pulp xylan was extracted in high purity in the first and second alkaline stage, respectively. Higher molecular mass xylan was obtained in the second than in the first alkaline extraction. Xylanase treatment before alkaline extraction hydrolyzed up to 12% of xylan to xylooligosaccharides. According to our results, preparation of polymeric xylan, and/or oligosaccharides as well as hemicellulose lean pulp with cellulose content of 93-94%, is possible by enzyme-aided alkaline extraction process.
authors: VTT Technical Research Centre of Finland, PO Box 1000, VTT, Finland. Terhi.Hakala@VTT.fi
authors: Terhi K
authors: TK
authors: Hakala
authors: Tiina
authors: T
authors: Liitiä
authors: Anna
authors: A
authors: Suurnäkki
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000737:chemistry; D005965:Glucuronates / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology; D014934:Wood / Q000737:chemistry*; D014990:Xylans / Q000737:chemistry / Q000302:isolation & purification*; D014995:Xylosidases / Q000737:chemistry
chemical_list: D000468:Alkalies; D005656:Fungal Proteins; D005965:Glucuronates; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; C570991:xylooligosaccharide; C007916:hemicellulose; D014995:Xylosidases; C056373:endoglucanase 2; D002480:Cellulase