title: Interplay between BDF1 and BDF2 and their roles in regulating the yeast salt stress response.
abstract: The homologous genes BDF1 and BDF2 in Saccharomyces cerevisiae encode bromodomain-containing transcription factors. Although double deletion of BDF1 and BDF2 is lethal, single deletion does not affect cell viability. The bdf2∆ cells showed normal growth upon salt stress. However, the absence of Bdf1p resulted in a salt-sensitive phenotype, and the salt sensitivity was suppressed by overexpression of BDF2. In this study, we further demonstrated that BDF2 shows dosage compensation in suppressing the salt sensitivity of bdf1∆. None of the tested domains replaced the function of intact Bdf1p. The 494-626 region in Bdf1p was more important than the other domains for salt resistance. In addition, Bdf1p negatively regulated the expression of BDF2 by binding its promoter at loci -387 to -48. However, Bdf2p did not affect the expression of BDF1. In addition, Bdf1p and its defective functional domain mutants could combine with Bdf2p. This physical interaction increased the salt tolerance of bdf1∆. The mitochondrial dysfunctions caused by BDF1 deletion were restored by overexpression of BDF2 under salt stress conditions.
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
authors: Jiafang
authors: J
authors: Fu
authors: Jin
authors: J
authors: Hou
authors: Liangyu
authors: L
authors: Liu
authors: Lei
authors: L
authors: Chen
authors: Mingpeng
authors: M
authors: Wang
authors: Yu
authors: Y
authors: Shen
authors: Zhaojie
authors: Z
authors: Zhang
authors: Xiaoming
authors: X
authors: Bao
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004303:Dosage Compensation, Genetic; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D050296:Microbial Viability; D008928:Mitochondria / Q000502:physiology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance; D013312:Stress, Physiological; D016385:TATA Box; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000092468:Bromodomain Containing Proteins
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C091342:BDF1 protein, S cerevisiae; D000092468:Bromodomain Containing Proteins; C581713:BDF2 protein, S cerevisiae