title: A phenolic antioxidant from the Pacific oyster (Crassostrea gigas) inhibits oxidation of cultured human hepatocytes mediated by diphenyl-1-pyrenylphosphine.
abstract: 3,5-Dihydroxy-4-methoxybenzyl alcohol (DHMBA), an antioxidant isolated from the Pacific oyster (Crassostrea gigas), was studied in a cell-based fluorometric antioxidant assay using human hepatocyte-derived cells (C3A) and diphenyl-1-pyrenylphosphine (DPPP) as a fluorescent probe. In comparison with two hydrophilic antioxidants, DHMBA showed the stronger inhibition of DPPP-mediated fluorescence than chlorogenic acid and l-ascorbic acid: at a concentration of 320 μM of DPPP, the inhibition was 26.4±2.6%, 11.1±1.2%, and 0±2.0% for DHMBA, chlorogenic acid, and l-ascorbic acid, respectively (mean±SD, n=4). Their relative oxygen radical absorbance capacities (ORAC) were dissociated with their cell-based antioxidant activities: 1.47±0.40, 4.57±0.30, and 0.53±0.13 μmol TE/μmol for DHMBA, chlorogenic acid, and l-ascorbic acid, respectively (mean±SD, n=4). The amphiphilicity of DHMBA was better than chlorogenic acid and l-ascorbic acid might underlie this dissociation. Since the C3A cells are human hepatoma-derived cells, DHMBA might be useful in the prevention and treatment of liver diseases by involving an oxidation process.
authors: Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060-0815, Japan.
authors: Mitsugu
authors: M
authors: Watanabe
authors: Hirotoshi
authors: H
authors: Fuda
authors: Shigeki
authors: S
authors: Jin
authors: Toshihiro
authors: T
authors: Sakurai
authors: Shu-Ping
authors: SP
authors: Hui
authors: Seiji
authors: S
authors: Takeda
authors: Takayuki
authors: T
authors: Watanabe
authors: Takao
authors: T
authors: Koike
authors: Hitoshi
authors: H
authors: Chiba
mesh_terms: D000818:Animals; D000840:Anisoles / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010125:Ostreidae / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D011721:Pyrenes / Q000494:pharmacology*
chemical_list: D000840:Anisoles; D000975:Antioxidants; D009943:Organophosphorus Compounds; D010636:Phenols; D011721:Pyrenes; C066322:diphenyl-1-pyrenylphosphine; C066943:anisyl alcohol