title: Clinical significance of quantitative analysis of plasma epstein- barr virus DNA in patients of Xinjiang Uygur nationality with Hodgkin's Lymphoma.
abstract: OBJECTIVE
  To investigate the relationship between plasma EBV-DNA concentration and clinicopathologic features of Hodgkin's lymphoma cases.
  
  
  METHODS
  At first, the positive rate of plasma EBV-DNA was determined with a nested-PCR method using 45 specimens from Uygur HL patients, as well as 110 healthy people sampled as normal controls. Secondly, using fluorescent quantitative nested-PCR, EBV viral load was assessed in the EBV-DNA positive plasma samples. Then, relationships between plasma EBV viral load and clinicopathologic features of HL patients were analyzed.
  
  
  RESULTS
  The positive rate of plasma EBV-DNA of HL patients was significantly higher than that of normal controls (53.3%vs26.4%, P=0.001). There was no significant difference about plasma EBV viral load between EBV-associated HL and EBV-DNA positive normal people (P=0.490). Looking at patients' characteristics, plasma EBV viral load in 10-20 years EBV-associated HL was higher than in EBV cases which were less than 10 years or more than 35 years (P=0.025). Furthermore, in EBV-associated HL, concentration of plasma EBV-DNA was significantly higher in advanced stage disease (stages III-IV; P=0.013), and with B-symptoms (P=0.020).
  
  
  CONCLUSIONS
  EBV-DNA levels were associated with part of clinicopathologic features of cases. It was of practical use to screen HL. Further etiological studies appear warranted.
authors: Department of Medical Oncology, Affiliated Tumor Hospital, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, China jingpingbai@yeah.net
authors: Xun
authors: X
authors: Li
authors: Shun-E
authors: SE
authors: Yang
authors: Yun-Quan
authors: YQ
authors: Guo
authors: Ming-Xia
authors: MX
authors: Shen
authors: Li
authors: L
authors: Gu
authors: Gulikezi Bing
authors: GB
authors: Zhao
authors: Wei
authors: W
authors: Liu
authors: Tuer
authors: T
authors: Xun
authors: Jing-Ping
authors: JP
authors: Bai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000097:blood; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000821:virology*; D005006:Ethnicity; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006689:Hodgkin Disease / Q000097:blood / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019562:Viral Load / Q000235:genetics; D055815:Young Adult
chemical_list: D004279:DNA, Viral