title: The proangiogenic phenotype of natural killer cells in patients with non-small cell lung cancer.
abstract: The tumor microenvironment can polarize innate immune cells to a proangiogenic phenotype. Decidual natural killer (dNK) cells show an angiogenic phenotype, yet the role for NK innate lymphoid cells in tumor angiogenesis remains to be defined. We investigated NK cells from patients with surgically resected non-small cell lung cancer (NSCLC) and controls using flow cytometric and functional analyses. The CD56(+)CD16(-) NK subset in NSCLC patients, which represents the predominant NK subset in tumors and a minor subset in adjacent lung and peripheral blood, was associated with vascular endothelial growth factor (VEGF), placental growth factor (PIGF), and interleukin-8 (IL-8)/CXCL8 production. Peripheral blood CD56(+)CD16(-) NK cells from patients with the squamous cell carcinoma (SCC) subtype showed higher VEGF and PlGF production compared to those from patients with adenocarcinoma (AdC) and controls. Higher IL-8 production was found for both SCC and AdC compared to controls. Supernatants derived from NSCLC CD56(+)CD16(-) NK cells induced endothelial cell chemotaxis and formation of capillary-like structures in vitro, particularly evident in SCC patients and absent from controls. Finally, exposure to transforming growth factor-β(1) (TGFβ(1)), a cytokine associated with dNK polarization, upregulated VEGF and PlGF in peripheral blood CD56(+)CD16(-) NK cells from healthy subjects. Our data suggest that NK cells in NSCLC act as proangiogenic cells, particularly evident for SCC and in part mediated by TGFβ(1).
authors: Scientific and Technology Park, IRCCS MultiMedica, Milan, Italy.
authors: Antonino
authors: A
authors: Bruno
authors: Chiara
authors: C
authors: Focaccetti
authors: Arianna
authors: A
authors: Pagani
authors: Andrea S
authors: AS
authors: Imperatori
authors: Marco
authors: M
authors: Spagnoletti
authors: Nicola
authors: N
authors: Rotolo
authors: Anna Rita
authors: AR
authors: Cantelmo
authors: Francesca
authors: F
authors: Franzi
authors: Carlo
authors: C
authors: Capella
authors: Guido
authors: G
authors: Ferlazzo
authors: Lorenzo
authors: L
authors: Mortara
authors: Adriana
authors: A
authors: Albini
authors: Douglas M
authors: DM
authors: Noonan
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000276:immunology / Q000378:metabolism*; D059016:Tumor Microenvironment / Q000276:immunology
chemical_list: C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1