title: Antitubercular diterpenoids from Vitex trifolia.
abstract: A new halimane diterpenoid, 13-hydroxy-5(10),14-halimadien-6-one (1) and two new labdane diterpenoids, 6α,7α-diacetoxy-13-hydroxy-8(9),14-labdadien (2) and 9-hydroxy-13(14)-labden-15,16-olide (3), were isolated for the first time, along with fifteen known compounds, from the hexane soluble fraction of methanolic extract of Vitex trifolia leaves. The structures of these new diterpenoids were elucidated by spectral analysis. Their relative configurations were established using analysis of NOESY correlations and coupling constants observed in (1)H NMR. Compounds 2, 3 and another known diterpenoid, isoambreinolide (4) were evaluated for antitubercular activity. 3 and 4 exhibited antitubercular activity (MIC=100 and 25 μg/ml) against Mycobacterium tuberculosis H37Rv in BACTEC-460 assay.
authors: Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants (Council of Scientific and Industrial Research), Lucknow, India.
authors: Neerja
authors: N
authors: Tiwari
authors: Jayprakash
authors: J
authors: Thakur
authors: Dharmendra
authors: D
authors: Saikia
authors: Madan M
authors: MM
authors: Gupta
mesh_terms: D000995:Antitubercular Agents / Q000737:chemistry / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D018515:Plant Leaves / Q000737:chemistry; D020950:Vitex / Q000737:chemistry*
chemical_list: C584402:13-hydroxy-5(10),14-halimadien-6-one; C584404:6alpha,7alpha-diacetoxy-13-hydroxy-8(9),14-labdadien; C584403:9-hydroxy-13(14)-labden-15,16-olide; D000995:Antitubercular Agents; D004224:Diterpenes