title: Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene.
abstract: The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.
authors: Hungarian Academy of Sciences Institute for Veterinary Medical Research, Centre for Agricultural Research P.O. Box 18 H-1581 Budapest Hungary.
authors: Boglárka
authors: B
authors: Sellyei
authors: Eva
authors: E
authors: Ivanics
authors: Tibor
authors: T
authors: Magyar
mesh_terms: D000818:Animals; D001717:Birds; D004269:DNA, Bacterial / Q000235:genetics; D010326:Pasteurella Infections; D016979:Pasteurella multocida; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004269:DNA, Bacterial