title: Postconditioning attenuates acute intestinal ischemia-reperfusion injury.
abstract: The aim of this study was to test the hypothesis that postconditioning (POC) would reduce the detrimental effects of the acute intestinal ischemia-reperfusion (I/R) compared to those of the abrupt onset of reperfusion. POC has a protective effect on intestinal I/R injury by inhibiting events in the early minutes of reperfusion in rats. Twenty-four Wistar-Albino rats were subjected to the occlusion of superior mesenteric artery for 30 minutes, then reperfused for 120 minutes, and randomized to the four different modalities of POC: (1) control (no intervention); (2) POC-3 (three cycles of 10 seconds of reperfusion-reocclusion, 1 minute total intervention); (3) POC-6 (six cycles of 10 seconds of reperfusion-reocclusion, 2 minutes total intervention); and (4) sham operation (laparotomy only). The arterial blood samples [0.3 mL total creatine kinase (CK) and 0.6 mL malondialdehyde (MDA)] and the intestinal mucosal MDA were collected from each after reperfusion. POC, especially POC-6, was effective in attenuating postischemic pathology by decreasing the intestinal tissue MDA levels, serum total CK activity, inflammation, and total histopathological injury scores. POC exerted a protective effect on the intestinal mucosa by reducing the mesenteric oxidant generation, lipid peroxidation, and neutrophil accumulation. The six-cycle algorithm demonstrated the best protection.
authors: Department of General Surgery, Giresun University Faculty of Medicine, Giresun, Turkey. dr.ilker52@mynet.com
authors: Ilker
authors: I
authors: Sengul
authors: Demet
authors: D
authors: Sengul
authors: Osman
authors: O
authors: Guler
authors: Adnan
authors: A
authors: Hasanoglu
authors: Mustafa Kemal
authors: MK
authors: Urhan
authors: Ahmet Sukru
authors: AS
authors: Taner
authors: Jakob
authors: J
authors: Vinten-Johansen
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D017538:Mesenteric Artery, Superior / Q000378:metabolism / Q000473:pathology*; D008641:Mesenteric Vascular Occlusion / Q000097:blood / Q000473:pathology*; D020556:Neutrophil Infiltration; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology*
chemical_list: D008315:Malondialdehyde; D003402:Creatine Kinase