title: Effects of Urtica dioica on oxidative stress, proliferation and apoptosis after partial hepatectomy in rats.
abstract: The present study was performed to investigate the effect of Urtica dioica (UD) on liver regeneration after partial hepatectomy (PH) in rats. A total of 24 male Sprague Dawley rats were divided into three groups: sham-operated, PH and PH + UD; each group contains eight animals. The rats in UD-treated groups were given UD oils (2 ml/kg/day) once a day orally for 7 days starting 3 days prior to hepatectomy operation. At day 7 after resection, liver samples were collected. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were estimated in liver homogenates. Moreover, histopathological examination, mitotic index (MI), proliferating cell nuclear antigen labeling, proliferation index (PI), transferase-mediated deoxyuridine triphosphate nick end-labeling assay, apoptotic index (AI) were evaluated at day 7 after hepatectomy. As a result, UD significantly increased MI and PI, significantly decreased AI and also attenuated hepatic vacuolar degeneration and sinusoidal congestion in PH rats. UD treatment significantly decreased the elevated tissue MDA level and increased the reduced SOD activity and GSH level in the tissues. These results suggest that UD pretreatment was beneficial for rat liver regeneration after partial hepatectomy.
authors: Department of General Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey.
authors: Serhat
authors: S
authors: Oguz
authors: Department of Histology and Embryology, Faculty of Medicine, Istanbul Medeniyet University, Istanbul, Turkey mkanter65@yahoo.com.
authors: Mehmet
authors: M
authors: Kanter
authors: Department of Histology and Embryology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors: Mustafa
authors: M
authors: Erboga
authors: Department of General Surgery, Istanbul Surgical Hospital, Istanbul, Turkey.
authors: Toygar
authors: T
authors: Toydemir
authors: Department of Emergency Medicine, Faculty of Medicine, Trakya University, Edirne, Turkey.
authors: Mustafa Burak
authors: MB
authors: Sayhan
authors: Department of Pediatrics, Istanbul Research and Training Hospital, Istanbul, Turkey.
authors: Hatice
authors: H
authors: Onur
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006498:Hepatectomy; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000187:drug effects; D008107:Liver Diseases / Q000188:drug therapy; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D028321:Plant Preparations / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D026982:Urtica dioica / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D028321:Plant Preparations; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: TUNEL; UD; immunohistochemistry; oxidative stress; partial hepatectomy; rat