title: Effects of ectopic HER-2/neu gene expression on the COX-2/PGE2/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells.
abstract: OBJECTIVE
  To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma.
  
  
  METHODS
  The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA.
  
  
  RESULTS
  Over-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells.
  
  
  CONCLUSIONS
  HER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal.
authors: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
authors: Shu
authors: S
authors: Li
authors: XiaoXin
authors: X
authors: Ma
authors: Li
authors: L
authors: Ma
authors: Cuicui
authors: C
authors: Wang
authors: YuanQi
authors: Y
authors: He
authors: ZhiJuan
authors: Z
authors: Yu
mesh_terms: D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D015232:Dinoprostone / Q000235:genetics / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D018734:Genes, erbB-2; D006801:Humans; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction; D014162:Transfection
chemical_list: D001141:Aromatase; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D018719:Erb-b2 Receptor Tyrosine Kinases; C496540:PTGS2 protein, human; C508053:ERBB2 protein, human