title: Rapid and specific post-synthesis modification of DNA through a biocompatible condensation of 1,2-aminothiols with 2-cyanobenzothiazole.
abstract: Post-synthesis modification of DNA is an important way of functionalizing DNA molecules. Herein, we describe a method that first enzymatically incorporates a cyanobenzothiazole (CBT)-modified thymidine. The side-chain handle CBT can undergo a rapid and site-specific cyclization reaction with 1,2-aminothiols to afford DNA functionalization in aqueous solution. Another key advantage of this method is the formation of a single stereo/regioisomer in the process, which allows for precise control of DNA modification to yield a single component for aptamer selection work and other applications.
authors: Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
authors: Yunfeng
authors: Y
authors: Cheng
authors: Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
authors: Hanjing
authors: H
authors: Peng
authors: Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
authors: Weixuan
authors: W
authors: Chen
authors: Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
authors: Nanting
authors: N
authors: Ni
authors: Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
authors: Bowen
authors: B
authors: Ke
authors: Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
authors: Chaofeng
authors: C
authors: Dai
authors: Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
authors: Binghe
authors: B
authors: Wang
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D057930:Click Chemistry; D003500:Cyclization; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D009570:Nitriles / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: C578355:2-cyanobenzothiazole; D052160:Benzothiazoles; D009570:Nitriles; D013438:Sulfhydryl Compounds; D004247:DNA