title: Preparation and characterization of a polystyrene/bovine serum albumin nanoparticle-coated capillary for chiral separation using open-tubular capillary electrochromatography.
abstract: Polystyrene (PS) nanoparticles coated by BSA, hereafter denoted as PS/BSA, were prepared and chemically immobilized for the first time onto a capillary inner wall for open-tubular CEC (OTCEC). EOF and scanning electron micrography were used to characterize the prepared nanoparticle-coated capillaries. To investigate the performance of the prepared columns in OTCEC, chiral separation of d,l-tryptophan (dl-Trp) was performed in monolayer BSA-modified capillary and PS/BSA nanoparticle-coated columns. The results indicated that the nanoparticle-modified column afforded a higher resolution compared with the monolayer type. Rapid enantioseparation of dl-Trp (within 3 min) was achieved with the PS/BSA-immobilized column using an electroosmotic pump-assisted CEC. Enantiomer separations of other compounds like dl-tyrosine and warfarin were also achieved with the column. Besides, run-to-run and column-to-column repeatabilities of the PS/BSA-coated column in the chiral separation were systematically introduced.
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, PR China.
authors: Dongmei
authors: D
authors: Wang
authors: Xiaojiao
authors: X
authors: Song
authors: Yin
authors: Y
authors: Duan
authors: Liang
authors: L
authors: Xu
authors: Jing
authors: J
authors: Zhou
authors: Hongquan
authors: H
authors: Duan
mesh_terms: D000818:Animals; D053801:Capillary Electrochromatography / Q000191:economics / Q000295:instrumentation*; D002417:Cattle; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011137:Polystyrenes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013237:Stereoisomerism; D014364:Tryptophan / Q000302:isolation & purification*
chemical_list: D011137:Polystyrenes; D012710:Serum Albumin, Bovine; D014364:Tryptophan