title: Evaluation of cyanocobalamin in multivitamin tablets and their Standard Reference Material 3280 by HPLC with visible detection.
abstract: As a part of the development of an alternative to microbiological assay for vitamin B12, we performed a quantitative analysis of cyanocobalamin (CN-cbl) in a National Institute of Standards and Technology Standard Reference Material (SRM 3280) by HPLC. Using this method, the observed value (4.64 microg/g) of CN-cbl in SRM 3280 was found to be in good agreement with the certified value (4.80 microg/g). The accuracy was over 95%, with a corresponding measurement precision value of 5%. To evaluate the applicability of the method on commercial multivitamin tablets, the method was applied to a variety of these samples. The present method has a good accuracy and precision to evaluate CN-cbl with respect to all of the examined tablets.
authors: National Institute of Health and Nutrition, Department of Food Function and Labeling, 1-23-1, Toyama, Shinjuku, Tokyo 162-8636, Japan. teruki@nih.go.jp
authors: Teruki
authors: T
authors: Matsumoto
authors: Jun
authors: J
authors: Takebayashi
authors: Yoshiko
authors: Y
authors: Ishimi
authors: Chihiro
authors: C
authors: Ozawa
authors: Akira
authors: A
authors: Sano
authors: Takashi
authors: T
authors: Hirota
authors: Kaori
authors: K
authors: Endoh
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D012015:Reference Standards; D012996:Solutions; D013607:Tablets; D014805:Vitamin B 12 / Q000032:analysis*; D014815:Vitamins / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D012996:Solutions; D013607:Tablets; D014815:Vitamins; D014805:Vitamin B 12