title: Biodegradation of petroleum hydrocarbons in the presence of nickel and cobalt.
abstract: Bioremediation of environments co-contaminated with hydrocarbons and heavy metals often pose a challenge as heavy metals exert toxicity to existing communities of hydrocarbon degraders. Multi-resistant bacterial strains were studied for ability to degrade hydrocarbons in chemically defined media amended with 5.0 mM Ni(2+), and Co(2+). The bacteria, Pseudomonas aeruginosa CA207Ni, Burkholderia cepacia AL96Co, and Corynebacterium kutscheri FL108Hg, utilized crude oil and anthracene without lag phase at specific growth rate spanning 0.3848-0.8259 per day. The bacterial populations grew in hydrocarbon media amended with nickel (Ni) and cobalt (Co) at 0.8393-1.801 days generation time (period of exponential growth, t = 15 days). The bacteria degraded 96.24-98.97, and 92.94-96.24% of crude oil, and anthracene, respectively, within 30 days without any impedance due to metal toxicity (at 5.0 mM). Rather, there was reduction of Ni and Co concentrations in the axenic culture 30 days post-inoculation to 0.08-0.12 and 0.11-0.15 mM, respectively. The metabolic functions of the bacteria are active in the presence of toxic metals (Ni and Co) while utilizing petroleum hydrocarbons for increase in biomass. These findings are useful to other baseline studies on decommissioning of sites co-contaminated with hydrocarbons and toxic metals.
authors: Department of Microbiology, University of Lagos, Akoka, Yaba, Lagos, Nigeria.
authors: Ganiyu Oladunjoye
authors: GO
authors: Oyetibo
authors: Matthew Olusoji
authors: MO
authors: Ilori
authors: Oluwafemi Sunday
authors: OS
authors: Obayori
authors: Olukayode Oladipo
authors: OO
authors: Amund
mesh_terms: D000873:Anthracenes / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000633:toxicity; D001711:Biotransformation; D016956:Burkholderia cepacia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003035:Cobalt / Q000378:metabolism* / Q000633:toxicity; D003352:Corynebacterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D024881:Drug Resistance, Bacterial; D006838:Hydrocarbons / Q000378:metabolism*; D019216:Metals, Heavy / Q000378:metabolism* / Q000633:toxicity; D009532:Nickel / Q000378:metabolism* / Q000633:toxicity; D010578:Petroleum / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D000900:Anti-Bacterial Agents; D003470:Culture Media; D006838:Hydrocarbons; D019216:Metals, Heavy; D010578:Petroleum; D003035:Cobalt; D009532:Nickel; C034020:anthracene
keywords: Anthracene; Bacteria; Biodegradation; Cobalt; Crude oil; Heavy metal; Nickel; Petroleum hydrocarbon