title: Evaluation of phytoremediation potential of Tagetes patula L. for the degradation of textile dye Reactive Blue 160 and assessment of the toxicity of degraded metabolites by cytogenotoxicity.
abstract: Tagetes patula is an annual flowering plant belonging to family Asteraceae. The present study deals with in vitro decolorization and remediation of a textile dye Reactive Blue 160 by T. patula. There was considerable (∼90%) decolorization of the dye within 4d of incubation, as confirmed by UV-vis, HPLC and FTIR analysis. The enzymes responsible for the remediation were lignin peroxidase, tyrosinase, laccase and NADH-DCIP reductase which were found in root tissues of the plantlets. GC-MS analysis of the products revealed formation of six metabolites such as sodium benzenesulfonate, 6-chloro 1,3,5-triazine-2,4-diamine, disodium benzene-1,4-disulfonate, sodium 3-amino-4-hydroxybenzenesulfonate, 1-phenylmethanediamine and sodium 4-amino-3-carboxybenzenesulfonate after phytoremediation of Reactive Blue 160. Based on the FTIR and GC-MS results, the possible pathway for the biodegradation of Reactive Blue 160 has been traced. The non-toxic nature of the degraded products was confirmed by performing cytogenotoxicity tests on root tip cells of growing Allium cepa.
authors: Department of Biochemistry, Shivaji University, Kolhapur 416 004, India.
authors: Asmita V
authors: AV
authors: Patil
authors: Jyoti P
authors: JP
authors: Jadhav
mesh_terms: D000490:Allium / Q000187:drug effects / Q000235:genetics; D001391:Azo Compounds / Q000378:metabolism* / Q000633:toxicity*; D001557:Benzenesulfonates / Q000378:metabolism* / Q000633:toxicity*; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism* / Q000633:toxicity*; D004249:DNA Damage; D031686:Tagetes / Q000378:metabolism*; D013783:Textile Industry
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; C584233:reactive blue 160