title: An amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan nanocomposite film for chlorpyrifos detection.
abstract: In this work, a novel amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan (MWCNTs-THI-CHIT) nanocomposite film as electrode modified material was developed for the detection of chlorpyrifos residues. The nanocomposite film was dropped onto a glassy carbon electrode (GCE), and then the anti-chlorpyrifos monoclonal antibody was covalently immobilized onto the surface of MWCNTs-THI-CHIT/GCE using the crosslinking agent glutaraldehyde (GA). The modification procedure was characterized by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, a linear relationship between the relative change in peak current of different pulse voltammetry (DPV) and the logarithm of chlorpyrifos solution concentration was obtained in the range from 0.1 to 1.0 × 10(5) ng/mL with a detection limit of 0.046 ng/mL. The proposed chlorpyrifos immunosensor exhibited high reproducibility, stability, and good selectivity and regeneration, making it a potential alternative tool for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.
authors: School of Agriculture and Food Engineering, Shandong University of Technology, No.12, Zhangzhou Road, Zibo 255049, China. sunxia2151@sina.com
authors: Xia
authors: X
authors: Sun
authors: Yaoyao
authors: Y
authors: Cao
authors: Zhili
authors: Z
authors: Gong
authors: Xiangyou
authors: X
authors: Wang
authors: Yan
authors: Y
authors: Zhang
authors: Jinmei
authors: J
authors: Gao
mesh_terms: D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry; D004390:Chlorpyrifos / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010640:Phenothiazines / Q000737:chemistry
chemical_list: D037742:Nanotubes, Carbon; D010640:Phenothiazines; D048271:Chitosan; D004390:Chlorpyrifos; C009469:thionine