title: Crosstalk between casein kinase II and Ste20-related kinase Nak1.
abstract: Although the sterile 20 (Ste20) serine/threonine protein kinase was originally identified as a component of the S. cerevisiae mating pathway, it has homologs in higher eukaryotes and is part of a larger family of Ste20-like kinases. Ste20-like kinases are involved in multiple cellular processes, such as cell growth, morphogenesis, apoptosis and immune response. Carrying out such a diverse array of biological functions requires numerous regulatory inputs and outputs in the form of protein-protein interactions and post-translational modifications. Hence, a thorough knowledge of Ste20-like kinase binding partners and phosphorylation sites will be essential for understanding the various roles of these kinases. Our recent study revealed that Schizosaccharomyces pombe Nak1 (a conserved member of the GC-kinase sub-family of Ste20-like kinases) is in a complex with the leucine-rich repeat-containing protein Sog2. Here, we show a novel and unexpected interaction between the Nak1-Sog2 kinase complex and Casein kinase 2 (Cka1, Ckb1 and Ckb2) using tandem-affinity purification followed by mass spectrometric analysis. In addition, we identify unique phosphosites on Nak1, Sog2 and the catalytic subunit of casein kinase 2, Cka1. Given the conserved nature of these kinases, we expect this work will shed light on the functions of these proteins both in yeast and higher eukaryotes.
authors: Max F. Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria.
authors: Lubos
authors: L
authors: Cipak
authors: Sneha
authors: S
authors: Gupta
authors: Iva
authors: I
authors: Rajovic
authors: Quan-Wen
authors: QW
authors: Jin
authors: Dorothea
authors: D
authors: Anrather
authors: Gustav
authors: G
authors: Ammerer
authors: Dannel
authors: D
authors: McCollum
authors: Juraj
authors: J
authors: Gregan
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D047390:Casein Kinase II / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D047390:Casein Kinase II; C500976:Nak1 protein, S pombe; D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C078754:STE20 protein, S cerevisiae
keywords: Ste20 kinase; casein kinase 2; fission yeast; polar growth