title: Centrobin regulates centrosome function in interphase cells by limiting pericentriolar matrix recruitment.
abstract: The amount of pericentriolar matrix at the centrosome is tightly linked to both microtubule nucleation and centriole duplication, although the exact mechanism by which pericentriolar matrix levels are regulated is unclear. Here we show that Centrobin, a centrosomal protein, is involved in regulating these levels. Interphase microtubule arrays in Centrobin-depleted cells are more focused around the centrosome and are less stable than the arrays in control cells. Centrobin-depleted cells initiate microtubule nucleation more rapidly than control cells and exhibit an increase in the number of growing microtubule ends emanating from the centrosome, while the parameters of microtubule plus end dynamics around the centrosome are not significantly altered. Finally, we show that Centrobin depletion results in the increased recruitment of pericentriolar matrix proteins to the centrosome, including γ-tubulin, AKAP450, Kendrin and PCM-1. We propose that Centrobin might regulate microtubule nucleation and organization by controlling the amount of pericentriolar matrix.
authors: Department of Cell and Molecular Biology, Queensland Institute of Medical Research, Brisbane, QLD, Australia.
authors: Jessie M
authors: JM
authors: Jeffery
authors: Ilya
authors: I
authors: Grigoriev
authors: Ina
authors: I
authors: Poser
authors: Armando
authors: A
authors: van der Horst
authors: Nicholas
authors: N
authors: Hamilton
authors: Nigel
authors: N
authors: Waterhouse
authors: Jonathan
authors: J
authors: Bleier
authors: V Nathan
authors: VN
authors: Subramaniam
authors: Ivan V
authors: IV
authors: Maly
authors: Anna
authors: A
authors: Akhmanova
authors: Kum Kum
authors: KK
authors: Khanna
mesh_terms: D054758:A Kinase Anchor Proteins; D001324:Autoantigens; D002148:Calmodulin-Binding Proteins; D018797:Cell Cycle Proteins / Q000172:deficiency / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000502:physiology; D002502:Centrioles / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D006367:HeLa Cells; D006801:Humans; D007399:Interphase / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D014404:Tubulin
chemical_list: D054758:A Kinase Anchor Proteins; C119719:AKAP9 protein, human; D001324:Autoantigens; C507203:CNTROB protein, human; D002148:Calmodulin-Binding Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D008869:Microtubule-Associated Proteins; C086074:PCM1 protein, human; D014404:Tubulin; C410004:kendrin
keywords: centriole duplication; centrobin; microtubules; nucleation; pericentriolar matrix