title: Arraying cell cultures using PEG-DMA micromolding in standard culture dishes.
abstract: A robust and effortless procedure is presented, which allows for the microstructuring of standard cell culture dishes. Cell adhesion and proliferation are controlled by three-dimensional poly(ethylene glycol)-dimethacrylate (PEG-DMA) microstructures. The spacing between microwells can be extended to millimeter size in order to enable the combination with robotic workstations. Cell arrays of microcolonies can be studied under boundary-free growth conditions by lift-off of the PEG-DMA layer in which the growth rate is accessible via the evolution of patch areas. Alternatively, PEG-DMA stencils can be used as templates for plasma-induced patterning.
authors: Fakultät für Physik, Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, 80539 München, Germany.
authors: Anna-Kristina
authors: AK
authors: Marel
authors: Susanne
authors: S
authors: Rappl
authors: Alicia
authors: A
authors: Piera Alberola
authors: Joachim Oskar
authors: JO
authors: Rädler
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D004285:Dogs; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D061985:Madin Darby Canine Kidney Cells; D008567:Membranes, Artificial; D008689:Methacrylates / Q000494:pharmacology*; D055616:Microtechnology / Q000295:instrumentation*; D058626:Plasma Gases / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D005353:Fibronectins; D008567:Membranes, Artificial; D008689:Methacrylates; D058626:Plasma Gases; C421283:poly(ethylene glycol)-dimethacrylate; D011092:Polyethylene Glycols