title: The onset of Triton X-100 solubilization of sphingomyelin/ceramide bilayers: effects of temperature and composition.
abstract: The early stages of Triton X-100 solubilization of bilayers consisting of sphingomyelin/ceramide (SM/Cer) mixtures have been studied using a combination of calorimetric and spectroscopic techniques. Compositions based on sphingomyelin, containing up to 30 mol% Cer, at 4, 20 and 50°C have been examined. The presence of Cer does not modify the affinity (in terms of ΔG of binding per mol total lipid) of the SM-based bilayers for Triton X-100, although it does increase the amount of detergent required for the onset of solubilization. At 50°C more detergent was required to solubilize the SM/Cer bilayers than at 20°C. The data can be rationalized in terms of lipid and detergent geometries and interactions (Lichtenberg et al., 2013).
authors: Unidad de Biofísica (Centro Mixto CSIC, UPV/EHU), Departamento de Bioquímica, Universidad del País Vasco, Bilbao, Spain.
authors: Hasna
authors: H
authors: Ahyayauch
authors: Cristina
authors: C
authors: Arnulphi
authors: Jesús
authors: J
authors: Sot
authors: Alicia
authors: A
authors: Alonso
authors: Félix M
authors: FM
authors: Goñi
mesh_terms: D002518:Ceramides / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D017830:Octoxynol / Q000737:chemistry*; D012995:Solubility; D013109:Sphingomyelins / Q000737:chemistry*; D013696:Temperature
chemical_list: D002518:Ceramides; D003902:Detergents; D008051:Lipid Bilayers; D013109:Sphingomyelins; D017830:Octoxynol