title: Monoclonal antibody-based fluorescence polarization immunoassay for high throughput screening of furaltadone and its metabolite AMOZ in animal feeds and tissues.
abstract: A simple and fast homogeneous fluorescent polarization immunoassay (FPIA) was developed for the determination of furaltadone and its metabolite 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ). Monoclonal antibody with high cross-reactivity to furaltadone and the nitrophenyl derivative of AMOZ (NPAMOZ) were produced against a novel immunogen and the effects of several synthesized tracers on FPIA sensitivity studied. The proposed FPIA, using an optimum antibody and tracer pair, had an IC₅₀ of 4.3 µg L⁻¹ and limit of detection at 0.6 µg L⁻¹ for furaltadone, and 2.7 µgL⁻¹ and 0.3 µg L⁻¹ for NPAMOZ. Recoveries of furaltadone from animal feeds by FPIA ranged from 79.6 to 87.7%, while recoveries of AMOZ from animal tissues ranged from 72.9 to 83.1%. Good correlation (R>0.99) between the results of this FPIA and a standard analytical method was obtained. The FPIA does not require separation or washing steps and the total time required for equilibrium of the antibody-tracer interaction is only 10 min. These results indicated that the proposed FPIA offers great potential and utility for the high throughput screening of furaltadone residues in animal feed and its metabolite AMOZ residues in animal tissues.
authors: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, No. 483, Wushan Road, Tianhe District, Guangzhou 510642, China.
authors: Zhen-Lin
authors: ZL
authors: Xu
authors: Shi-Wei
authors: SW
authors: Zhang
authors: Yuan-Ming
authors: YM
authors: Sun
authors: Yu-Dong
authors: YD
authors: Shen
authors: Hong-Tao
authors: HT
authors: Lei
authors: Yue-Ming
authors: YM
authors: Jiang
authors: Sergei A
authors: SA
authors: Eremin
authors: Jin-Yi
authors: JY
authors: Yang
authors: Hong
authors: H
authors: Wang
mesh_terms: D000821:Animal Feed; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002645:Chickens; D005260:Female; D005399:Fishes; D016231:Fluorescence Polarization Immunoassay; D057166:High-Throughput Screening Assays / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D060172:Morpholinos / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics*; D009581:Nitrofurans / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D013552:Swine; D014018:Tissue Distribution
chemical_list: C000590728:3-amino-5-methylmorpholino-2-oxazolidinone; D000911:Antibodies, Monoclonal; D060172:Morpholinos; D009581:Nitrofurans; D023303:Oxazolidinones; C007795:furaltadon