title: Effect of eicosapentaenoic acid-derived prostaglandin E3 on intestinal epithelial barrier function.
abstract: Prostaglandins (PG) are inflammatory mediators derived from arachidonic or eicosapentaenoic acid giving rise to the 2-series or the 3-series prostanoids, respectively. Previously, we have observed that PGE2 disrupts epithelial barrier function. Considering the beneficial effect of fish oil consumption in intestinal inflammatory processes, the aim of this study was to assess the role of PGE3 on epithelial barrier function assessed from transepithelial electrical resistance and dextran fluxes in Caco-2 cells. The results indicate that PGE3 increased paracellular permeability (PP) to the same extent as PGE2, through the interaction with EP1 and EP4 receptors and with intracellular Ca(2+) and cAMP as the downstream targets. Moreover, we observed a redistribution of tight junction proteins, occludin and claudin-4. In conclusion, PGE3 is able to increase PP thus leading to reconsider the role of PGE2/PGE3 ratio in the beneficial effects of dietary fish oil supplementation in the disruption of barrier function.
authors: Departament de Fisiologia, Facultat de Farmàcia, Av. Joan XXIII s/n, 08028 Barcelona, Spain.
authors: Maria J
authors: MJ
authors: Rodríguez-Lagunas
authors: Ruth
authors: R
authors: Ferrer
authors: Juan J
authors: JJ
authors: Moreno
mesh_terms: D000527:Alprostadil / Q000031:analogs & derivatives* / Q000378:metabolism; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability / Q000502:physiology; D015232:Dinoprostone / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*
chemical_list: C033246:prostaglandin E3; D015118:Eicosapentaenoic Acid; D000527:Alprostadil; D015232:Dinoprostone