title: Mast cell stabilizing effect of (-)-Elema-1,3,11(13)-trien-12-ol and thujopsene from Thujopsis dolabrata is mediated by down-regulation of interleukin-4 secretion in antigen-induced RBL-2H3 cells.
abstract: Several isolated compounds from the wood part of Thujopsis dolabrata were evaluated for their inhibitory effects against antigen-induced mast cell degranulation and interleukin-4 (IL-4) secretion, as well as IL-4 mRNA and protein expression in immunoglobulin E (IgE)-sensitized RBL-2H3 cells. Among the five isolated compounds, (-)-elema-1,3,11(13)-trien-12-ol (1) and thujopsene (2) exhibited the potent inhibitory activity against mast cell degranulation measured by β-hexosaminidase release with IC values of 27.4 µM and 25.1 µM, respectively. These compounds also inhibited the release of IL-4 (IC values of 7.0, 6.7 µM, respectively), IL-4 mRNA expression (IC values of 16.5, 7.2 µM, respectively) and IL-4 protein expression (IC values of 17.0, 9.6 µM, respectively) in antigen-induced IgE-sensitized RBL-2H3 cells. These results suggested that (-)-elema-1,3,11(13)-trien-12-ol (1) and thujopsene (2) effectively inhibits mast cell degranulation as well as IL-4 production, suggesting that these compounds from Thujopsis dolabrata can be used as candidates for IgE-mediated allergic disorders.
authors: Natural Products Research Institute, College of Pharmacy, Seoul National University.
authors: Chul-Hee
authors: CH
authors: Kim
authors: Taehun
authors: T
authors: Lee
authors: Ikhoon
authors: I
authors: Oh
authors: Kung-Woo
authors: KW
authors: Nam
authors: Kyeong Ho
authors: KH
authors: Kim
authors: Ki-Bong
authors: KB
authors: Oh
authors: Jongheon
authors: J
authors: Shin
authors: Woongchon
authors: W
authors: Mar
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D045744:Cell Line, Tumor; D029781:Cupressus / Q000737:chemistry*; D015536:Down-Regulation; D007073:Immunoglobulin E / Q000276:immunology*; D015847:Interleukin-4 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D012717:Sesquiterpenes / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D012717:Sesquiterpenes; C582951:elema-1,3,11(13)-trien-12-ol; D015847:Interleukin-4; D007073:Immunoglobulin E; C433908:thujopsene; D001619:beta-N-Acetylhexosaminidases