title: Cytotoxicity and uptake of archaeosomes prepared from Aeropyrum pernix lipids.
abstract: Archaeon Aeropyrum pernix K1 is an obligate aerobic hyperthermophilic organism with C25,25-archeol membrane lipids with head groups containing inositol. Interactions of archaeosomes, liposomes prepared from lipids of A. pernix, with mammalian cells in vitro were studied. In vitro cytotoxicity was tested on five different cell lines: rodent mouse melanoma cells (B16-F1) and Chinese hamster ovary (CHO) cells, and three human cell lines-epithelial colorectal adenocarcinoma cells (CACO-2), liver hepatocellular carcinoma cell line (Hep G2) and endothelial umbilical vein cell line (EA.hy926). Archaeosomes were nontoxic to human Hep G2, CACO-2 and mildly toxic to rodent CHO and B16-F1 cells but showed strong cytotoxic effect on EA.hy926 cells. Confocal microscopy revealed that archaeosomes are taken up by endocytosis. The uptake of archaeosomes and the release of loaded calcein are more prominent in EA.hy926 cells, which is in line with high toxicity toward these cells. The mechanisms of uptake, release and action in these cells as well as in vivo functioning have to be further studied for possible targeted drug delivery.
authors: Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Ljubljana, TrŽaška, Ljubljana, Slovenia.
authors: T B
authors: TB
authors: Napotnik
authors: J
authors: J
authors: Valant
authors: D
authors: D
authors: Gmajner
authors: S
authors: S
authors: Passamonti
authors: D
authors: D
authors: Miklavčič
authors: N P
authors: NP
authors: Ulrih
mesh_terms: D041522:Aeropyrum / Q000737:chemistry* / Q000254:growth & development; D000818:Animals; D018533:Biomass; D016466:CHO Cells; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D004705:Endocytosis / Q000187:drug effects*; D042783:Endothelial Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D008081:Liposomes; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
chemical_list: D004337:Drug Carriers; D008055:Lipids; D008081:Liposomes
keywords: Aeropyrum pernix; archaeosomes; cytotoxicity; endocytosis