title: ChIP-PED enhances the analysis of ChIP-seq and ChIP-chip data.
abstract: BACKGROUND
  Although chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) or tiling array hybridization (ChIP-chip) is increasingly used to map genome-wide-binding sites of transcription factors (TFs), it still remains difficult to generate a quality ChIPx (i.e. ChIP-seq or ChIP-chip) dataset because of the tremendous amount of effort required to develop effective antibodies and efficient protocols. Moreover, most laboratories are unable to easily obtain ChIPx data for one or more TF(s) in more than a handful of biological contexts. Thus, standard ChIPx analyses primarily focus on analyzing data from one experiment, and the discoveries are restricted to a specific biological context.
  
  
  RESULTS
  We propose to enrich this existing data analysis paradigm by developing a novel approach, ChIP-PED, which superimposes ChIPx data on large amounts of publicly available human and mouse gene expression data containing a diverse collection of cell types, tissues and disease conditions to discover new biological contexts with potential TF regulatory activities. We demonstrate ChIP-PED using a number of examples, including a novel discovery that MYC, a human TF, plays an important functional role in pediatric Ewing sarcoma cell lines. These examples show that ChIP-PED increases the value of ChIPx data by allowing one to expand the scope of possible discoveries made from a ChIPx experiment.
  
  
  BACKGROUND
  http://www.biostat.jhsph.edu/~gewu/ChIPPED/
authors: Department of Biostatistics, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA.
authors: George
authors: G
authors: Wu
authors: Jason T
authors: JT
authors: Yustein
authors: Matthew N
authors: MN
authors: McCall
authors: Michael
authors: M
authors: Zilliox
authors: Rafael A
authors: RA
authors: Irizarry
authors: Karen
authors: K
authors: Zeller
authors: Chi V
authors: CV
authors: Dang
authors: Hongkai
authors: H
authors: Ji
mesh_terms: D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D014157:Transcription Factors