title: The effects of low-level laser irradiation on cellular viability and proliferation of human skin fibroblasts cultured in high glucose mediums.
abstract: Delayed wound healing is one of the most challenging complications of diabetes mellitus (DM) in clinical medicine. This study has aimed to evaluate the effects of low-level laser therapy (LLLT) on human skin fibroblasts (HSFs) cultured in a high glucose concentration. HSFs were cultured either in a concentration of physiologic glucose (5.5 mM/l) or high glucose media (11.1 and 15 mM/l) for either 1 or 2 weeks after which they were subsequently cultured in either the physiologic glucose or high concentration glucose media during laser irradiation. LLLT was carried out with a helium-neon (He-Ne) laser unit at energy densities of 0.5, 1, and 2 J/cm(2), and power density of 0.66 mW/cm(2) on 3 consecutive days. HSFs' viability and proliferation rate were evaluated with the dimethylthiazol-diphenyltetrazolium bromide (MTT) assay. The LLLT at densities of 0.5 and 1 J/cm(2) had stimulatory effects on the viability and proliferation rate of HSFs cultured in physiologic glucose (5.5 mM/l) medium compared to their control cultures (p = 0.002 and p = 0.046, respectively). All three doses of 0.5, 1, and 2 J/cm(2) had stimulatory effects on the proliferation rate of HSFs cultured in high glucose concentrations when compared to their control cultures (p = 0.042, p = 0.000, and p = 0.000, respectively). This study showed that HSFs originally cultured for 2 weeks in high glucose concentration followed by culture in physiologic glucose during laser irradiation showed enhanced cell viability and proliferation. Thus, LLLT had a stimulatory effect on these HSFs.
authors: Dental Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Mohammad
authors: M
authors: Esmaeelinejad
authors: Mohammad
authors: M
authors: Bayat
authors: Hasan
authors: H
authors: Darbandi
authors: Mehrnoush
authors: M
authors: Bayat
authors: Nariman
authors: N
authors: Mosaffa
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D048430:Cell Shape / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D003470:Culture Media / Q000737:chemistry; D048909:Diabetes Complications / Q000473:pathology / Q000532:radiotherapy; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D005947:Glucose / Q000032:analysis; D006801:Humans; D054020:Lasers, Gas / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D008954:Models, Biological; D012867:Skin / Q000166:cytology / Q000528:radiation effects*; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D003470:Culture Media; D005947:Glucose