title: Comparative analysis of DNA methylation polymorphism in drought sensitive (HPKC2) and tolerant (HPK4) genotypes of horse Gram (Macrotyloma uniflorum).
abstract: DNA methylation is known as an epigenetic modification that affects gene expression in plants. Variation in CpG methylation behavior was studied in two natural horse gram (Macrotyloma uniflorum [Lam.] Verdc.) genotypes, HPKC2 (drought-sensitive) and HPK4 (drought-tolerant). The methylation pattern in both genotypes was studied through methylation-sensitive amplified polymorphism. The results revealed that methylation was higher in HPKC2 (10.1%) than in HPK4 (8.6%). Sequencing demonstrated sequence homology with the DRE binding factor (cbf1), the POZ/BTB protein, and the Ty1-copia retrotransposon among some of the polymorphic fragments showing alteration in methylation behavior. Differences in DNA methylation patterns could explain the differential drought tolerance and the epigenetic signature of these two horse gram genotypes.
authors: Plant Metabolic Engineering Laboratory, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, 176061 Himachal Pradesh, India.
authors: Jyoti
authors: J
authors: Bhardwaj
authors: Monika
authors: M
authors: Mahajan
authors: Sudesh Kumar
authors: SK
authors: Yadav
mesh_terms: D003001:Cloning, Molecular; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements; D018744:DNA, Plant / Q000235:genetics; D055864:Droughts; D044127:Epigenesis, Genetic; D007887:Fabaceae / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic; D011485:Protein Binding; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D018744:DNA, Plant