title: ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue.
abstract: We describe a clearing method for enhanced visualization of cell morphology and connections in neuronal and non-neuronal tissue. Using Clear(T) or Clear(T2), which are composed of formamide or formamide/polyethylene glycol, respectively, embryos, whole mounts and thick brain sections can be rapidly cleared with minimal volume changes. Unlike other available clearing techniques, these methods do not use detergents or solvents, and thus preserve lipophilic dyes, fluorescent tracers and immunohistochemical labeling, as well as fluorescent-protein labeling.
authors: Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 14-509 P&S, New York, NY 10032, USA.
authors: Takaaki
authors: T
authors: Kuwajima
authors: Austen A
authors: AA
authors: Sitko
authors: Punita
authors: P
authors: Bhansali
authors: Chris
authors: C
authors: Jurgens
authors: William
authors: W
authors: Guido
authors: Carol
authors: C
authors: Mason
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D003584:Cytological Techniques / Q000379:methods*; D003902:Detergents / Q000009:adverse effects; D004210:Dissection / Q000379:methods*; D004622:Embryo, Mammalian; D007202:Indicators and Reagents / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D012997:Solvents / Q000009:adverse effects; D013048:Specimen Handling / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
chemical_list: D003902:Detergents; D007202:Indicators and Reagents; D012997:Solvents