title: Identification and characterization of miRNA transcriptome in potato by high-throughput sequencing.
abstract: Micro RNAs (miRNAs) represent a class of short, non-coding, endogenous RNAs which play important roles in post-transcriptional regulation of gene expression. While the diverse functions of miRNAs in model plants have been well studied, the impact of miRNAs in crop plant biology is poorly understood. Here we used high-throughput sequencing and bioinformatics analysis to analyze miRNAs in the tuber bearing crop potato (Solanum tuberosum). Small RNAs were analysed from leaf and stolon tissues. 28 conserved miRNA families were found and potato-specific miRNAs were identified and validated by RNA gel blot hybridization. The size, origin and predicted targets of conserved and potato specific miRNAs are described. The large number of miRNAs and complex population of small RNAs in potato suggest important roles for these non-coding RNAs in diverse physiological and metabolic pathways.
authors: The James Hutton Institute, Invergowrie, Dundee, United Kingdom.
authors: Runxuan
authors: R
authors: Zhang
authors: David
authors: D
authors: Marshall
authors: Glenn J
authors: GJ
authors: Bryan
authors: Csaba
authors: C
authors: Hornyik
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D055029:Inverted Repeat Sequences; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics*; D018547:Plant Stems / Q000235:genetics*; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics*; D017423:Sequence Analysis, RNA; D011198:Solanum tuberosum / Q000235:genetics*; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant