title: Photo-activated DNA binding and antimicrobial activities of alkaloids from Glycosmis pentaphylla.
abstract: In our screening for photosensitizers from natural resources, four alkaloids were isolated from Glycosmis pentaphylla by various chromatography techniques. Their structures were identified as glycoborinine (1), glybomine B (2), carbalexin A (3) and N-p-coumaroyltyramine (4) by spectral analysis. Their photoactivated antimicrobial activities were evaluated by thin-layer chromatography (TLC) agar overlay assay against Staphylococcus aureus and Bacillus subtilis. It was found that compounds 1 and 4 showed photo-activated antimicrobial activities. Meantime, photo-activated DNA binding activities of these compounds were also assessed by using a specially prepared 1.8 kb DNA fragment and restriction enzymes. Under UVA irradiation, compound 1 showed moderate inhibition on Nde I, Xba I, Nco I and Bcl I which have either 5'-TpA or 5'-ApT and trace or no inhibition on other restriction enzymes. It showed a similar inhibition pattern with the reference 8-methoxypsoralen. However, compounds 2-4 showed no inhibition against any of the restriction enzymes.
authors: College of Chemistry and Material Sciences, South Central University for Nationalities, Wuhan 430074, China.
authors: Yu
authors: Y
authors: Chen
authors: Bo
authors: B
authors: Yang
authors: Jing
authors: J
authors: Xu
authors: Tong
authors: T
authors: Zheng
authors: Hua
authors: H
authors: Fan
authors: Guang-Zhong
authors: GZ
authors: Yang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects*; D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D053938:DNA Fragmentation; D004262:DNA Restriction Enzymes / Q000378:metabolism; D008027:Light; D015394:Molecular Structure; D017319:Photosensitizing Agents / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011485:Protein Binding; D027821:Rutaceae / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects*; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D002227:Carbazoles; D003373:Coumaric Acids; C001411:N-(4-hydroxy-beta-phenethyl)-4-hydroxycinnamide; D017319:Photosensitizing Agents; C493076:glybomine B; C584906:glycoborinine; D004262:DNA Restriction Enzymes