title: A universal and multiplex kinase assay using γ-[(18)O(4)]-ATP.
abstract: We have synthesized γ-[(18)O4]-ATP and used it to develop a non-radioactive and multiplex method. Significantly, this novel approach can be applied to any kinases without using a purified enzyme or a fluorescent substrate. Using this approach, the effectiveness and specificity of inhibitors on several kinases could be readily determined.
authors: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, PR China.
authors: Chuan
authors: C
authors: Fu
authors: Xueyun
authors: X
authors: Zheng
authors: Yao
authors: Y
authors: Jiang
authors: Yan
authors: Y
authors: Liu
authors: Pengxiang
authors: P
authors: Xu
authors: Zhiping
authors: Z
authors: Zeng
authors: Rihe
authors: R
authors: Liu
authors: Yufen
authors: Y
authors: Zhao
mesh_terms: D051698:Activating Transcription Factor 2 / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism; D001681:Biological Assay; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D048308:Mitogen-Activated Protein Kinase 14 / Q000378:metabolism; D027361:Organic Anion Transporters / Q000378:metabolism; D010104:Oxygen Radioisotopes; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011494:Protein Kinases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D051698:Activating Transcription Factor 2; D000255:Adenosine Triphosphate; D048308:Mitogen-Activated Protein Kinase 14; D027361:Organic Anion Transporters; D010104:Oxygen Radioisotopes; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; C497105:ATF2 protein, human; C487009:SLCO6A1 protein, human