title: A link between fertility and K+ homeostasis: role of the renal H,K-ATPase type 2.
abstract: Renal K(+) retention is activated during pregnancy through a mechanism unknown to date. Here, we showed that the renal stimulation of H,K-ATPase type 2 (HKA2), whose expression was recently identified to be progesterone-dependent, is part of the mechanism favoring K(+) accumulation during gestation. Moreover, investigation of the gestational phenotype of HKA2-null mice compared to their wild-type (WT) littermate revealed a decrease in fertility (gestation was successful in 33 % of HKA2-null mice vs. 83 % of WT mice) and in litter size (6.5 ± 0.6 and 7.8 ± 0.4 fetuses per litter, respectively). We also observed that urinary K(+) excretion decreased by 20 % and plasma K(+) concentration rose slightly (11 %) in WT mice during gestation (relative to basal conditions). In contrast, the renal excretion of K(+) and plasma K(+) levels in HKA2-null mice remained constant during gestation, whereas fecal K(+) excretion increased. As a consequence, HKA2-null mice did not accumulate K(+) in their extracellular compartment as efficiently as WT mice did. Finally, the link between inefficient K(+) balance adaptations and gestational complications was established when we observed that these complications could be reversed with an increased K(+) uptake. Altogether, these results define a novel physiological role for the HKA2 transporter and uncover a link between K(+) metabolism and fertility.
authors: UPMC Univ Paris 6/INSERM/CNRS UMRS 872 Equipe 3 Laboratoire de Génomique, Physiologie et Physiopathologie Rénales ERL 7226, 75006, Paris, France.
authors: Amel
authors: A
authors: Salhi
authors: Christine
authors: C
authors: Lamouroux
authors: Nikolay B
authors: NB
authors: Pestov
authors: Nikolaï N
authors: NN
authors: Modyanov
authors: Alain
authors: A
authors: Doucet
authors: Gilles
authors: G
authors: Crambert
mesh_terms: D000818:Animals; D005260:Female; D005298:Fertility / Q000502:physiology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D007668:Kidney / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011188:Potassium / Q000378:metabolism / Q000652:urine; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism / Q000503:physiopathology
chemical_list: D017506:H(+)-K(+)-Exchanging ATPase; D011188:Potassium