title: [Subpopulation content of lymphocytes in peripheral blood in patients with diabetes mellitus type 2 and obesity].
abstract: In the present study was determined the relative quantity subpopulations of lymphocytes, activated T-lymphocytes and CD95-antigen (Fas/APO-1) expression on lymphocytes in the peripheral blood of patients with diabetes mellitus type 2 (DM 2) and obesity in comparison with the healthy persons. The cells were analyzed by the method of flow cytometry using Beckman Coulter FC 500 cytometer. The following of cells subsets: CD19+, CD3+, CD3+CD4+, CD3+CD8+, CD3-CD16+CD56+, CD3+CD16+CD56+, CD3+CD25+, CD3+HLA-DR+, CD45+CD95+ were investigated. In this research was establish the rise of immunoregulatory index (CD3+CD4+/CD3+CD8+) in consequence of decrease the percentages of cytotoxic T-lymphocytes in patients with DM2 and obesity in comparison with the healthy persons. In the peripheral blood of patients with DM2 and obesity were observed a greater level of activated T-lymphocytes (CD3+HLA-DR+), that reflect the increase activity of T-cell immunity. The direct correlation was found between the increased quantity of NKT-cells (CD3+CD16+CD56+) and contain of triglicerides in the peripheral blood of patients with DM2. In these patients also was noted a greater level of lymphocytes expression of CD95-antigen in comparison with the patients with obesity and healthy persons. These results suggest that immune mechanisms play the important role in pathogenesesis of DM2 and obesity.
authors: É N
authors: ÉN
authors: Trushina
authors: O K
authors: OK
authors: Mustafina
authors: S Kh
authors: SKh
authors: Soto
authors: T B
authors: TB
authors: Sentsova
authors: O A
authors: OA
authors: Plotnikova
authors: R I
authors: RI
authors: Alekseeva
authors: Kh Kh
authors: KhKh
authors: Sharafetdinov
authors: I Iu
authors: IIu
authors: Semenchenko
authors: V D
authors: VD
authors: Kuznetsov
mesh_terms: D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D019014:fas Receptor / Q000276:immunology*
chemical_list: D015415:Biomarkers; C491901:FAS protein, human; D019014:fas Receptor