title: Methyl Antcinate A suppresses the population of cancer stem-like cells in MCF7 human breast cancer cell line.
abstract: Methyl antcinate A (MAA) is an ergostane-type triterpenoid extracted from the fruiting bodies of Antrodia camphorate that has been reported to be a cytotoxic agent towards some types of cancer cells, such as oral cancer and liver cancer. Cancer stem cells (CSCs) are a particular population within cancer cells which are responsible for tumor initiation, drug resistance and metastasis and targeting CSCs is an emerging area in cancer therapy. In this study, we examine the effect of MAA on cancer stem-like cells in the MCF7 human breast cancer cell line. Although MAA displayed very low cytotoxic effect towards MCF7 under normal culture conditions, it did show good inhibitory effects on the self-renewal capability which was examined by mammosphere culture including primary and secondary sphere. MAA also inhibited cell migration ability of MCF7 sphere cells. By western blot analysis, MAA was shown to suppress the expression of heat shock protein 27 and increase the expression of IkBα and p53. In conclusion, our data demonstrate that MAA has anti-CSC activity and is worthy of future development of potent anticancer agents.
authors: School of Dentistry, Chung Shan Medical University, Taichung 402, Taiwan. cyp@csmu.edu.tw
authors: Chih-Yu
authors: CY
authors: Peng
authors: Pin-Chung
authors: PC
authors: Fong
authors: Cheng-Chia
authors: CC
authors: Yu
authors: Wan-Chi
authors: WC
authors: Tsai
authors: Yew-Min
authors: YM
authors: Tzeng
authors: Wen-Wei
authors: WW
authors: Chang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D061986:MCF-7 Cells; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D018874:Spheroids, Cellular; D014315:Triterpenes / Q000494:pharmacology* / Q000633:toxicity; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D055551:HSP27 Heat-Shock Proteins; D020672:I-kappa B Proteins; D014315:Triterpenes; D016159:Tumor Suppressor Protein p53; C553655:methyl antcinate A