title: Polysaccharides from peach pulp: structure and effects on mouse peritoneal macrophages.
abstract: Pulp from peaches contained polygalacturonic acid and arabinogalactan as main polysaccharides, which were isolated and characterised. The polygalacturonic acid (AE-CWI) contained 95% GalA and its (13)C NMR spectrum showed signals at δ 98.9, 78.0, 71.4, 69.1, 68.4, and 175.1 from C-1, C-4, C-5, C-3, C-2, and C-6 respectively, from (1→4)-linked α-GalpA units. Methylation-MS analysis of carboxy-reduced material (AE-CWI-CR) gave 90% of 2,3,6-Me(3)-galactitol acetate. The arabinogalactan (AE-AG) was composed mainly of Ara (41%) and Gal (50%) and was characterised (methylation analysis and (13)C NMR) as a type II-arabinogalactan. It induced peritoneal macrophage activation in mice, ~70% of cells treated with this fraction (1-50 μg/mL) having morphology of activated cells. However, NO production in macrophages treated with AE-AG was not affected. This suggests a new biological activity for peach polysaccharides.
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil.
authors: F F
authors: FF
authors: Simas-Tosin
authors: A P R
authors: AP
authors: Abud
authors: C C
authors: CC
authors: De Oliveira
authors: P A J
authors: PA
authors: Gorin
authors: G L
authors: GL
authors: Sassaki
authors: D F
authors: DF
authors: Bucchi
authors: M
authors: M
authors: Iacomini
mesh_terms: D000818:Animals; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D051379:Mice; D015394:Molecular Structure; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*
chemical_list: D005685:Galactans; D010936:Plant Extracts; D010368:Pectins; C005653:arabinogalactan; C003181:polygalacturonic acid