title: Omega-3 polyunsaturated fatty acids preserve retinal function in type 2 diabetic mice.
abstract: OBJECTIVE
  Diabetic retinopathy (DR) is associated with hyperglycemia-driven microvascular pathology and neuronal compromise in the retina. However, DR is also linked to dyslipidemia. As omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) are protective in proliferative retinopathy, we investigated the capacity of ω-3PUFAs to preserve retinal function in a mouse model of type 2 diabetes mellitus (T2DM).
  
  
  METHODS
  Male leptin-receptor-deficient (db/db) mice were maintained for 22 weeks (4 weeks-26 weeks of life) on calorically and compositionally matched diets, except for 2% enrichment in either ω-3 or ω-6PUFAs. Visual function was assessed at 9, 14 and 26 weeks by electroretinography. Retinal capillary and neuronal integrity, as well as glucose challenge responses, were assessed on each diet.
  
  
  RESULTS
  The ω-3PUFA diet significantly preserved retinal function in the mouse model of T2DM to levels similar to those observed in nondiabetic control mice on normal chow. Conversely, retinal function gradually deteriorated in db/db mice on a ω-6PUFA-rich diet. There was also an enhanced ability of ω-3PUFA-fed mice to respond to glucose challenge. The protection of visual function appeared to be independent of cytoprotective or anti-inflammatory effects of ω-3PUFAs.
  
  
  CONCLUSIONS
  This study identifies beneficial effects of dietary ω-3PUFAs on visual function in T2DM. The data are consistent with dyslipidemia negatively impacting retinal function. As ω-3PUFA lipid dietary interventions are readily available, safe and inexpensive, increasing ω-3PUFA intake in diabetic patients may slow the progression of vision loss in T2DM.
authors: 1] Department of Ophthalmology, Harvard Medical School, Children's Hospital Boston, Boston, MA, USA [2] Department of Ophthalmology, Maisonneuve-Rosemont Hospital Research Centre, University of Montreal, Montreal, Quebec, Canada.
authors: P
authors: P
authors: Sapieha
authors: J
authors: J
authors: Chen
authors: A
authors: A
authors: Stahl
authors: M R
authors: MR
authors: Seaward
authors: T L
authors: TL
authors: Favazza
authors: A M
authors: AM
authors: Juan
authors: C J
authors: CJ
authors: Hatton
authors: J-S
authors: JS
authors: Joyal
authors: N M
authors: NM
authors: Krah
authors: R J
authors: RJ
authors: Dennison
authors: J
authors: J
authors: Tang
authors: T S
authors: TS
authors: Kern
authors: J D
authors: JD
authors: Akula
authors: L E H
authors: LE
authors: Smith