title: Anti-adipogenic diarylheptanoids from Alnus hirsuta f. sibirica on 3T3-L1 cells.
abstract: A new diarylheptanoid, (5S)-hydroxy-1-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)-hepta-1E-en-3-one (1), was isolated along with seventeen known diarylheptanoids (2-18) from the methanol extract of Alnus hirsuta f. sibirica leaves using bioactivity-guided fractionation. Among the isolated compounds, compounds 1 and 2 and 4-12 reduced lipid accumulation dose-dependently in 3T3-L1 preadipocytes. Of the compounds active in the present assay system, the most potent compound 7, platyphyllonol-5-O-β-d-xylopyranoside, significantly suppressed the induction of peroxisome proliferator activated receptor γ (PPARγ and CCAAT/enhancer binding protein α (C/EBPα) protein expression, and inhibited adipocyte differentiation induced by troglitazone, a PPARγ agonist. It was demonstrated that compound 7 has anti-adipogenic activity mediated by the regulation of PPARγ dependent pathways.
authors: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Daehak-Dong, San 56-1, Sillim-Dong, Gwanak-Gu, Seoul 151-742, Republic of Korea.
authors: Mina
authors: M
authors: Lee
authors: Ji Yeon
authors: JY
authors: Song
authors: Young-Won
authors: YW
authors: Chin
authors: Sang Hyun
authors: SH
authors: Sung
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D029661:Alnus / Q000737:chemistry*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002839:Chromans / Q000494:pharmacology; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D015394:Molecular Structure; D047495:PPAR gamma / Q000819:agonists / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013329:Structure-Activity Relationship; D045162:Thiazolidinediones / Q000494:pharmacology; D000077288:Troglitazone
chemical_list: D002839:Chromans; D036381:Diarylheptanoids; D047495:PPAR gamma; D010936:Plant Extracts; D045162:Thiazolidinediones; D000077288:Troglitazone