title: Effect of immobilization on rotator cuff reconstruction with acellular dermal matrix grafts in an animal model.
abstract: BACKGROUND
  Using a rat model we determined whether immobilization improves tendon-to-bone healing and tendon maturation after rotator cuff reconstruction with acellular dermal matrix (ADM) grafts.
  
  
  METHODS
  Sprague-Dawley rats were randomly assigned to 3 equal groups. All rats were subjected to rotator cuff reconstruction and their shoulder was not immobilized (N-IM controls) or immobilized for 2- or 6 weeks immediately thereafter (2- and 6-IM groups, respectively). After detaching the rotator cuff including the full-thickness supraspinatus tendon at its insertion on the greater tuberosity, a defect was created. ADM patches were used for rotator cuff reconstruction. Specimens obtained 2, 6, and 12 weeks after surgery were subjected to semiquantitative histomorphological measurements to assess cellularity, vascularity, and collagen organization. In addition, specimens at 12 weeks were submitted for biomechanical analysis.
  
  
  RESULTS
  Cell density decreased over time in all 3 groups; there was no significant difference among the groups at 2 weeks. However, the 6-IM group harbored more cells in the tendon-to-bone interface than the 2-IM group at 6 weeks and the N-IM group at 12 weeks. Vascularity in the interface decreased over time in the N-IM and 2-IM groups but not the 6-IM rats. At 6 and 12 weeks, the 2-IM group manifested better collagen organization than the other groups. The 2-IM group exhibited higher ultimate load-to-failure than the other groups. Stiffness was similar in the 3 groups.
  
  
  CONCLUSIONS
  Remodeling of ADM grafts placed in rat rotator cuff defects was promoted by 2- but not 6-week immobilization.
authors: Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
authors: Keiji
authors: K
authors: Uezono
authors: Junji
authors: J
authors: Ide
authors: Takuya
authors: T
authors: Tokunaga
authors: Hidetoshi
authors: H
authors: Sakamoto
authors: Nobukazu
authors: N
authors: Okamoto
authors: Hiroshi
authors: H
authors: Mizuta
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D004195:Disease Models, Animal; D007103:Immobilization / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000070636:Rotator Cuff Injuries; D016038:Skin Transplantation; D013708:Tendon Injuries / Q000628:therapy*; D014945:Wound Healing
keywords: Animal Model; Basic Science; Biomechanics/Histology; Rotator cuff reconstruction; acellular dermal matrix graft; immobilization; tendon-to-bone healing