title: Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost.
abstract: Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60-65°C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, α-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications.
authors: Département de Chimie-Biologie, Université du Québec à Trois-Rivières, 3351 Boul. Des Forges, C.P. 500, Trois-Rivières (Québec), G9A 5H7 Canada.
authors: David M
authors: DM
authors: Charbonneau
authors: Fatma
authors: F
authors: Meddeb-Mouelhi
authors: Maurice
authors: M
authors: Boissinot
authors: Marc
authors: M
authors: Sirois
authors: Marc
authors: M
authors: Beauregard
keywords: 16S rDNA; Hydrolytic enzymes; Manure compost; Thermal stability; Thermophilic bacteria