title: Chemically induced intestinal damage models in zebrafish larvae.
abstract: Several intestinal damage models have been developed using zebrafish, with the aim of recapitulating aspects of human inflammatory bowel disease (IBD). These experimentally induced inflammation models have utilized immersion exposure to an array of colitogenic agents (including live bacteria, bacterial products, and chemicals) to induce varying severity of inflammation. This technical report describes methods used to generate two chemically induced intestinal damage models using either dextran sodium sulfate (DSS) or trinitrobenzene sulfonic acid (TNBS). Methods to monitor intestinal damage and inflammatory processes, and chemical-genetic methods to manipulate the host response to injury are also described.
authors: Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland, New Zealand.
authors: Stefan H
authors: SH
authors: Oehlers
authors: Maria Vega
authors: MV
authors: Flores
authors: Christopher J
authors: CJ
authors: Hall
authors: Kazuhide S
authors: KS
authors: Okuda
authors: John Oliver
authors: JO
authors: Sison
authors: Kathryn E
authors: KE
authors: Crosier
authors: Philip S
authors: PS
authors: Crosier
mesh_terms: D000423:Alcian Blue / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D016264:Dextran Sulfate / Q000494:pharmacology*; D004195:Disease Models, Animal; D055808:Drug Discovery; D004760:Enterocolitis / Q000139:chemically induced* / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology; D005434:Flow Cytometry; D006801:Humans; D007101:Immersion; D007249:Inflammation / Q000139:chemically induced* / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced* / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology; D053759:Interleukin-23 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D007814:Larva; D008845:Microinjections; D060172:Morpholinos / Q000008:administration & dosage; D009499:Neutral Red / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism; D061848:Optical Imaging; D016133:Polymerase Chain Reaction; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D053759:Interleukin-23; D060172:Morpholinos; D029961:Zebrafish Proteins; D009499:Neutral Red; D009569:Nitric Oxide; D014302:Trinitrobenzenesulfonic Acid; D016264:Dextran Sulfate; D000423:Alcian Blue