title: Quantitative real-time expression profiling of aquaporins-isoforms and growth response of Brassica juncea under arsenite stress.
abstract: The present study analyzed the expression level of aquaporins of plasma membrane intrinsic protein (PIP) class in response to arsenite (AsIII) exposure of 100 μM from 0.5 h to 8 days in Brassica juncea. The expression levels of most of the PIPs were down-regulated during the course of AsIII exposure. This led to decrease in total water content of plants, which in turn hampered seedling growth. The level of reactive oxygen species (superoxide radicals and hydrogen peroxide), lipid peroxidation and root oxidizability increased significantly upon exposure to AsIII as compared to that of control leading to an increase in cell death. The study proposes that the down-regulation of PIPs happened presumably to regulate AsIII levels, which, however, occurred at the cost of reduced growth, disturbed water balance and induced oxidative stress.
authors: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, Maharashtra, India. sudhakar.srivastava@gmail.com
authors: S
authors: S
authors: Srivastava
authors: A K
authors: AK
authors: Srivastava
authors: P
authors: P
authors: Suprasanna
authors: S F
authors: SF
authors: D'Souza
mesh_terms: D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D018053:Arsenites / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D009149:Mustard Plant / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D018384:Oxidative Stress; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D020033:Protein Isoforms / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics; D014867:Water / Q000737:chemistry
chemical_list: D020346:Aquaporins; D018053:Arsenites; D010940:Plant Proteins; D020033:Protein Isoforms; C110065:major intrinsic protein, plant; D014867:Water; D006861:Hydrogen Peroxide; C015001:arsenite