title: Effect of experimental diabetic retinopathy on the non-image-forming visual system.
abstract: Diabetic retinopathy is a leading cause of blindness. Intrinsically photosensitive retinal ganglion cells (ipRGCs), which express the photopigment melanopsin, are involved in non-image-forming visual responses such as photoentrainment of circadian rhythms and pupillary light reflex. Since several reports indicate that retinal ganglion cells are affected by diabetes, we investigated the non-image-forming visual system in an advanced stage of experimental diabetes in rats induced by streptozotocin. After 15 wks of diabetes induction, clear alterations in the visual function were observed and all animals developed mature cataracts. At this time point, concomitantly with a significant decrease in the number of Brn3a(+) retinal ganglion cells, no differences in the number of melanopsin-containing cells, melanopsin levels, and retinal projections to the suprachiasmatic nuclei and the olivary pretectal nucleus were observed. At high light intensity, afferent pupil light reflex appears to be conserved in diabetic animals. After 15 wks of diabetes induction, a significant decrease in light-induced c-Fos expression in the suprachiasmatic nuclei was found. In diabetic animals, the locomotor activity pattern was conserved, although a delay in the time needed for re-entrainment after a phase delay was observed. In diabetic animals, lensectomy reversed the alterations in c-Fos expression and in the locomotor activity rhythm. These results suggest that the neuronal substrate of the non-image-forming visual system remained largely unaffected at advanced stages of diabetes, and that lensectomy, a relatively easy and safe surgery, could partially restore circadian alterations induced by diabetes.
authors: Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental, Departamento de Bioquímica Humana, Facultad de Medicina/CEFyBO, Universidad de Buenos Aires/CONICET, Buenos Aires, Argentina.
authors: Diego C
authors: DC
authors: Fernandez
authors: Pablo H
authors: PH
authors: Sande
authors: Nuria
authors: N
authors: de Zavalía
authors: Nicolás
authors: N
authors: Belforte
authors: Damián
authors: D
authors: Dorfman
authors: Leandro P
authors: LP
authors: Casiraghi
authors: Diego
authors: D
authors: Golombek
authors: Ruth E
authors: RE
authors: Rosenstein
mesh_terms: D000818:Animals; D002772:Cholera Toxin; D002940:Circadian Rhythm; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D003930:Diabetic Retinopathy / Q000473:pathology*; D004596:Electroretinography; D005074:Evoked Potentials, Visual / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D016762:Genes, fos; D008297:Male; D009799:Ocular Physiological Phenomena; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000502:physiology; D017299:Rod Opsins / Q000235:genetics / Q000378:metabolism; D000099210:Melanopsin
chemical_list: D002772:Cholera Toxin; D017299:Rod Opsins; D000099210:Melanopsin