title: Respiratory infection of mice with mammalian reoviruses causes systemic infection with age and strain dependent pneumonia and encephalitis.
abstract: BACKGROUND
  Because mammalian reoviruses are isolated from the respiratory tract we modeled the natural history of respiratory infection of adult and suckling mice with T1 Lang (T1L) and T3 Dearing (T3D) reoviruses.
  
  
  METHODS
  Adult and suckling Balb/c mice were infected by the intranasal route and were assessed for dose response of disease as well as viral replication in the lung and other organs. Viral antigen was assessed by immunofluorescence and HRP staining of tissue sections and histopathology was assessed on formalin fixed, H + E stained tissue sections.
  
  
  RESULTS
  Intranasal infection of adult mice resulted in fatal respiratory distress for high doses (10(7) pfu) of T1L but not T3D. In contrast both T1L and T3D killed suckling mice at moderate viral dosages (10(5) pfu) but differed in clinical symptoms where T1L induced respiratory failure and T3D caused encephalitis. Infections caused transient viremia that resulted in spread to peripheral tissues where disease correlated with virus replication, and pathology. Immunofluorescent staining of viral antigens in the lung showed reovirus infection was primarily associated with alveoli with lesser involvement of bronchiolar epithelium. Immunofluorescent and HRP staining of viral antigens in brain showed infection of neurons by T3D and glial cells by T1L.
  
  
  CONCLUSIONS
  These mouse models of reovirus respiratory infection demonstrated age and strain dependent disease that are expected to be relevant to understanding and modulating natural and therapeutic reovirus infections in humans.
authors: Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd, Ottawa, Ontario K1H 8M5, Canada.
authors: Lianne
authors: L
authors: Gauvin
authors: Steffany
authors: S
authors: Bennett
authors: Hong
authors: H
authors: Liu
authors: Mansoureh
authors: M
authors: Hakimi
authors: Michael
authors: M
authors: Schlossmacher
authors: Jay
authors: J
authors: Majithia
authors: Earl G
authors: EG
authors: Brown
mesh_terms: D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D000956:Antigens, Viral / Q000276:immunology*; D001921:Brain / Q000473:pathology / Q000821:virology; D002460:Cell Line; D004195:Disease Models, Animal; D018792:Encephalitis, Viral / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D030102:Orthoreovirus, Mammalian / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D011024:Pneumonia, Viral / Q000473:pathology / Q000821:virology*; D012088:Reoviridae Infections / Q000473:pathology / Q000821:virology*; D012141:Respiratory Tract Infections / Q000473:pathology / Q000821:virology*; D013045:Species Specificity; D013997:Time Factors; D014766:Viremia; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral