title: Low SP1 expression differentially affects intestinal-type compared with diffuse-type gastric adenocarcinoma.
abstract: Specificity protein 1 (SP1) is an essential transcription factor that regulates multiple cancer-related genes. Because aberrant expression of SP1 is related to cancer development and progression, we focused on SP1 expression in gastric carcinoma and its correlation with disease outcomes. Although patient survival decreased as SP1 expression increased (P<0.05) in diffuse-type gastric cancer, the lack of SP1 expression in intestinal-type gastric cancer was significantly correlated with poor survival (P<0.05). The knockdown of SP1 in a high SP1-expressing intestinal-type gastric cell line, MKN28, increased migration and invasion but decreased proliferation. Microarray data in SP1 siRNA-transfected MKN28 revealed that the genes inhibiting migration were downregulated, whereas the genes negatively facilitating proliferation were increased. However, both migration and invasion were decreased by forced SP1 expression in a low SP1-expressing intestinal-type gastric cell line, AGS. Unlike the intestinal-type, in a high SP1-expressing diffuse-type gastric cell line, SNU484, migration and invasion were decreased by SP1 siRNA. In contrast to previous studies that did not identify differences between the 2 histological types, our results reveal that low expression of SP1 is involved in cancer progression and metastasis and differentially affects intestinal-type compared with diffuse-type gastric adenocarcinoma.
authors: Research Institute of Pharmaceutical Science, College of Pharmacy, Seoul National University, Seoul, Korea.
authors: Hun Seok
authors: HS
authors: Lee
authors: Cheol-Keun
authors: CK
authors: Park
authors: Ensel
authors: E
authors: Oh
authors: Özgür Cem
authors: ÖC
authors: Erkin
authors: Hun Soon
authors: HS
authors: Jung
authors: Mi-Hyun
authors: MH
authors: Cho
authors: Mi Jeong
authors: MJ
authors: Kwon
authors: Seoung Wan
authors: SW
authors: Chae
authors: Seok-Hyung
authors: SH
authors: Kim
authors: Li-Hui
authors: LH
authors: Wang
authors: Min-Jeong
authors: MJ
authors: Park
authors: Su-Yeon
authors: SY
authors: Lee
authors: Ho Bin
authors: HB
authors: Yang
authors: Lina
authors: L
authors: Jia
authors: Yoon-La
authors: YL
authors: Choi
authors: Young Kee
authors: YK
authors: Shin
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D016329:Sp1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D016019:Survival Analysis; D014162:Transfection
chemical_list: D016329:Sp1 Transcription Factor