title: Mass spectrometry-based methods to study protein architecture and dynamics.
abstract: Mass spectrometry is now an indispensable tool in the armamentarium of molecular biophysics, where it is used for tasks ranging from protein sequencing and mapping of post-translational modifications to studies of higher order structure, conformational dynamics, and interactions of proteins with small molecule ligands and other biopolymers. This mini-review highlights several popular mass spectrometry-based tools that are now commonly used for structural studies of proteins beyond their covalent structure with a particular emphasis on hydrogen exchange and direct electrospray ionization mass spectrometry.
authors: Department of Chemistry, University of Massachusetts-Amherst, Amherst, Massachusetts, USA. Kaltashov@chem.umass.edu
authors: Igor A
authors: IA
authors: Kaltashov
authors: Cedric E
authors: CE
authors: Bobst
authors: Rinat R
authors: RR
authors: Abzalimov
mesh_terms: D000818:Animals; D041961:Deuterium Exchange Measurement / Q000379:methods; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D011506:Proteins