title: NITRILASE1 regulates the exit from proliferation, genome stability and plant development.
abstract: Nitrilases are highly conserved proteins with catabolic activity but much less understood functions in cell division and apoptosis. To elucidate the biological functions of Arabidopsis NITRILASE1, we characterized its molecular forms, cellular localization and involvement in cell proliferation and plant development. We performed biochemical and mass spectrometry analyses of NITRILASE1 complexes, electron microscopy of nitrilase polymers, imaging of developmental and cellular distribution, silencing and overexpression of nitrilases to study their functions. We found that NITRILASE1 has an intrinsic ability to form filaments. GFP-NITRILASE1 was abundant in proliferating cells, distributed in cytoplasm, in the perinuclear area and associated with microtubules. As cells exited proliferation and entered differentiation, GFP-NITRILASE1 became predominantly nuclear. Nitrilase silencing dose-dependently compromised plant growth, led to loss of tissue organization and sustained proliferation. Cytokinesis was frequently aborted, leading to enlarged polyploid cells. In reverse, independently transformed cell lines overexpressing GFP-NITRILASE1 showed slow growth and increased rate of programmed cell death. Altogether, our data suggest that NITRILASE1 homologues regulate the exit from cell cycle and entry into differentiation and simultaneously are required for cytokinesis. These functions are essential to maintain normal ploidy, genome stability and tissue organization.
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Anna
authors: A
authors: Doskočilová
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Lucie
authors: L
authors: Kohoutová
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Jindřich
authors: J
authors: Volc
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Hana
authors: H
authors: Kourová
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Oldřich
authors: O
authors: Benada
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Jana
authors: J
authors: Chumová
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Ondřej
authors: O
authors: Plíhal
authors: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR, v. v. i., Sokolovská 6, 772 00, Olomouc, Czech Republic.
authors: Beáta
authors: B
authors: Petrovská
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Petr
authors: P
authors: Halada
authors: Plant Molecular Sciences, Centre for Systems and Synthetic Biology, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK.
authors: László
authors: L
authors: Bögre
authors: Institute of Microbiology AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors: Pavla
authors: P
authors: Binarová
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics; D000619:Aminohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D002453:Cell Cycle / Q000235:genetics; D016923:Cell Death / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D042822:Genomic Instability; D009363:Neoplasm Proteins / Q000235:genetics; D034622:RNA Interference; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D000619:Aminohydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase; C021867:nitrilase