title: Use of retinyl acetate, retinoic acid and insulin-like growth factor-I (IGF-I) to enhance goat embryo production.
abstract: Experiments were carried out to investigate the beneficial effects of retinyl acetate (RAc) and retinoic acid (RA) on goat oocyte maturation as well as the effects of insulin-like growth factor-I (IGF-I), RAc and RA during embryo culture under chemically defined conditions. In Experiment 1, in vitro maturation (IVM) was performed in a chemically defined basic maturation medium (bMM) supplemented with 0.3 μM RAc or 0.5 μM RA. Presumptive zygotes and embryos (2-4 cells) were cultured in droplets of potassium simplex optimised medium (KSOM); however, none of the embryos reached the blastocyst stage. In Experiment 2, oocytes were matured in bMM + RAc or bMM + RA. Presumptive zygotes and 2- to 4-cell embryos were placed in fresh KSOM droplets supplemented with RAc, RA, IGF-I, RAc+IGF-I or RA+IGF-I. In Experiment 1, addition of RAc and RA to bMM increased (P < 0.05) the proportion of 2- to 4-cell embryos reaching the morula stage as compared to the control. In Experiment 2, supplementation of embryo culture media with retinoids and IGF-I increased (P < 0.05) the proportion of 2- to 4-cell stage embryos developing to the morula and blastocyst stage. Our data demonstrate that goat embryo production in chemically defined media could be improved by exogenous RAc or RA and by the interaction between retinoids and IGF-I, and that goat embryos can be produced in vitro from oocytes following protocols similar to those currently used for cattle.
authors: Universidade Federal do Rio Grande do Norte Natal/RN Brazil.
authors: Adauto
authors: A
authors: Chiamenti
authors: Cristiano Rocha Aguiar
authors: CR
authors: Filho
authors: Marcelo Tigre
authors: MT
authors: Moura
authors: Fabíola Freitas
authors: FF
authors: Paula-Lopes
authors: Jairo Pereira
authors: JP
authors: Neves
authors: Cícero Cerqueira Cavalcanti
authors: CC
authors: Neto
authors: Paulo Bayard Dias
authors: PB
authors: Gonçalves
authors: Paulo Fernandes
authors: PF
authors: Lima
authors: Marcos Antonio Lemos
authors: MA
authors: Oliveira
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D047108:Embryonic Development; D005307:Fertilization in Vitro / Q000662:veterinary; D006041:Goats; D007334:Insulin-Like Growth Factor I; D014212:Tretinoin
chemical_list: D003470:Culture Media; D014212:Tretinoin; D007334:Insulin-Like Growth Factor I