title: Analysis of intracellular human immunodeficiency virus (HIV)-1 drug resistance mutations in multi-failed HIV-1-infected patients treated with a salvage regimen: 72-week follow-up.
abstract: The human immunodeficiency virus (HIV) mutational archive of proviral DNA was monitored during a 72-week follow-up in 20 multidrug-experienced HIV-1-infected patients treated with a darunavir/ritonavir-based salvage therapy. At the beginning of the study, all patients harboured a number of intracellular drug resistance-associated mutations (RAMs) in peripheral blood mononuclear cells. In some patients, a significant fluctuation in the number of RAMs was observed during the observation period. However, all patients, notwithstanding the presence or the fluctuation of intracellular RAMs, showed a persistently undetectable viraemia. The data suggest that the archived resistant viral variants change during suppressive therapy, but that the variants are unable to re-emerge and to affect virological response.
authors: Department of Molecular Medicine, Sapienza University, Rome, Italy.
authors: F
authors: F
authors: Falasca
authors: C
authors: C
authors: Montagna
authors: P
authors: P
authors: Maida
authors: M
authors: M
authors: Bucci
authors: A
authors: A
authors: Fantauzzi
authors: I
authors: I
authors: Mezzaroma
authors: G
authors: G
authors: Antonelli
authors: O
authors: O
authors: Turriziani
mesh_terms: D000328:Adult; D000368:Aged; D019380:Anti-HIV Agents / Q000627:therapeutic use*; D004279:DNA, Viral / Q000235:genetics; D024882:Drug Resistance, Viral; D005260:Female; D005500:Follow-Up Studies; D014644:Genetic Variation; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D016879:Salvage Therapy / Q000379:methods*; D017211:Treatment Failure
chemical_list: D019380:Anti-HIV Agents; D004279:DNA, Viral