title: Development of a highly sensitive and specific monoclonal antibody-based enzyme-linked immunosorbent assay for determination of doxycycline in chicken muscle, liver and egg.
abstract: A modified indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed using a highly sensitive and specific monoclonal antibody (McAb) to determine doxycycline (DC) residues in chicken tissues and egg. The McAb against DC was produced by hybridoma technique and a modified ic-ELISA was characterised in terms of sensitivity, specificity, precision and accuracy. At optimal experimental conditions, the standard curve was constructed at concentrations ranged from 0.01 to 100 ng/ml. The IC(50) value was 1.32±0.18 ng/ml. The limit of detection was 0.14±0.02 ng/g. The recoveries of DC from spiked chicken liver, muscle, and egg at levels of 50-600 ng/g were 84.6-85.5%, 88.2-89.1%, and 84.4-89.3%, respectively. The coefficient variations (CVs) were 5.1-9.3%, 3.7-11.3%, and 4.7-9.8%, respectively. Linear regression analysis showed good correlation, with r(2) values 0.9909 for chicken liver and 0.9916 for chicken muscle.
authors: College of Life Science, Chongqing Normal University, Chongqing 401331, PR China. hnxylt@163.com
authors: Tao
authors: T
authors: Le
authors: Zhengwu
authors: Z
authors: Zhao
authors: Wei
authors: W
authors: Wei
authors: Dingren
authors: D
authors: Bi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000032:analysis; D002645:Chickens; D004318:Doxycycline / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D008099:Liver / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D004318:Doxycycline