title: Rapid and efficient generation of neurons from human pluripotent stem cells in a multititre plate format.
abstract: Existing protocols for the generation of neurons from human pluripotent stem cells (hPSCs) are often tedious in that they are multistep procedures involving the isolation and expansion of neural precursor cells, prior to terminal differentiation. In comparison to these time-consuming approaches, we have recently found that combined inhibition of three signaling pathways, TGFβ/SMAD2, BMP/SMAD1, and FGF/ERK, promotes rapid induction of neuroectoderm from hPSCs, followed by immediate differentiation into functional neurons. Here, we have adapted our procedure to a novel multititre plate format, to further enhance its reproducibility and to make it compatible with mid-throughput applications. It comprises four days of neuroectoderm formation in floating spheres (embryoid bodies), followed by a further four days of differentiation into neurons under adherent conditions. Most cells obtained with this protocol appear to be bipolar sensory neurons. Moreover, the procedure is highly efficient, does not require particular expert skills, and is based on a simple chemically defined medium with cost-efficient small molecules. Due to these features, the procedure may serve as a useful platform for further functional investigation as well as for cell-based screening approaches requiring human sensory neurons or neurons of any type.
authors.affiliation: Max Planck Institute for Molecular Biomedicine, Germany.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.forename: Hans R
authors.initials: HR
authors.lastname: Schöler
authors.forename: Boris
authors.initials: B
authors.lastname: Greber
mesh_terms: D000577:Amides / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D016764:Cell Polarity / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000737:chemistry; D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D004338:Drug Combinations; D006801:Humans; D007797:Laminin / Q000737:chemistry; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D011509:Proteoglycans / Q000737:chemistry; D011725:Pyridines / Q000494:pharmacology; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D000577:Amides; D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D011725:Pyridines; C059485:matrigel; C108830:Y 27632; D011142:Polyvinyl Alcohol; D003094:Collagen