title: Lactobacillus plantarum 2142 prevents intestinal oxidative stress in optimized in vitro systems.
abstract: Recently, there has been a growing interest to replace antibiotics' administration with the application of probiotics. The aim of our investigations was to reveal the influence of spent culture supernatant of Lactobacillus plantarum 2142 on the response of enterocytes to oxidative stress, and the spent culture supernatant's ability to protect them from oxidative injury. The experiments were performed on non-carcinogenic porcine epithelial cell line, IPEC-J2 isolated from a neonatal piglet and on human colon adenocarcinoma cell line, Caco-2. The cells cultured on membrane inserts were treated with millimolar hydrogen peroxide solution to provoke oxidative stress. The peroxide-triggered cell response profile was evaluated via determination of change in transepithelial electrical resistance, quantification of extent of cell death by 4',6-diamidino-2 phenylindole (DAPI) staining and via estimation of proinflammatory cytokine, IL-8 production using ELISA technique. Non-starter lactobacilli supernatant-mediated inhibition of peroxide-triggered upregulation of IL-8 production confirmed the antiinflammatory properties of active metabolites produced by Lactobacillus plantarum 2142 in acute oxidative stress.
authors.affiliation: Szent István University Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences Budapest Hungary Szent István University Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences István u. 2 H-1078 Budapest Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Paszti-Gere
authors.forename: E
authors.initials: E
authors.lastname: Csibrik-Nemeth
authors.forename: K
authors.initials: K
authors.lastname: Szeker
authors.forename: R
authors.initials: R
authors.lastname: Csizinszky
authors.forename: O
authors.initials: O
authors.lastname: Palocz
authors.forename: O
authors.initials: O
authors.lastname: Farkas
authors.forename: P
authors.initials: P
authors.lastname: Galfi
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology; D048191:Lactiplantibacillus plantarum; D018384:Oxidative Stress / Q000187:drug effects*; D019936:Probiotics / Q000494:pharmacology*; D013552:Swine
chemical_list: D016209:Interleukin-8; D006861:Hydrogen Peroxide