title: A new anti-inflammatory triterpene saponin isolated from Anabasis setifera.
abstract: A bio-guided fractionation of Anabasis setifera Moq. (Chenopodiaceae) for anti-inflammatory activity was carried out using carrageenin rat paw edema model in rats. On the basis of percent edema inhibition after 3 h of carrageenin injection, n-butanol fraction showed promising activity through a significant (p < 0.05) decrease in paw volume by 85.6 % from control using indomethacin as reference standard. Moreover, the n-butanol fraction significantly (p < 0.05) decreased PGE2 and TNF-α in the exudates of rat paw edema. Chemical investigation of n-butanol fraction afforded α-amyrin 3-O-glucopyranoside (1), patuletin 7-O-glucopyranoside (2), myricitrin (3) and a new oleanane triterpene saponin derivative (4), sophradiol 3-O-α-L-(1)C4-rhamnopyranosyl-(1'''→4'')-O-β-D-(4)C1-galactopyranosyl (1''→6')-O-β-D-(4)C1-glucopyranoside. The structure of the new compound was determined by comprehensive analyses of their 1D and 2D NMR, mass spectral data and comparison with previously known analogs. Only compound 4 revealed a significant (p < 0.05) inhibition of cyclooxygenase 1 and 2 (COX) activities.
authors.affiliation: Medicinal Chemistry Department, Theodor Bilharz Research Institute (TBRI), Giza 12411, Egypt.
authors.forename: Allia M
authors.initials: AM
authors.lastname: Abdou
authors.forename: Hossam M
authors.initials: HM
authors.lastname: Abdallah
authors.forename: Mona A
authors.initials: MA
authors.lastname: Mohamed
authors.forename: Ghada A
authors.initials: GA
authors.lastname: Fawzy
authors.forename: Ashraf B
authors.initials: AB
authors.lastname: Abdel-Naim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002351:Carrageenan; D019663:Chenopodiaceae / Q000737:chemistry*; D051545:Cyclooxygenase 1 / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; D002351:Carrageenan; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2