title: Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium.
abstract: Strain IK-1(T) was isolated from decaying tissues of the shrub Wikstroemia oahuensis collected on O'ahu, Hawai'i. Cells were rods that stained Gram-negative. Gliding motility was not observed. The strain was oxidase-negative and catalase-positive. Zeaxanthin was the major carotenoid. Flexirubin-type pigments were not detected. The most abundant fatty acids in whole cells of IK-1(T) grown on R2A were iso-C(15:0) and one or both of C(16:1)ω7c and C(16:1)ω6c. Based on comparisons of the nucleotide sequence of the 16S rRNA gene, the closest neighbouring type strains were Flavobacterium rivuli WB 3.3-2(T) and Flavobacterium subsaxonicum WB 4.1-42(T), with which IK-1(T) shares 93.84 and 93.67% identity, respectively. The G+C content of the genomic DNA was 44.2 mol%. On the basis of distance from its nearest phylogenetic neighbours and phenotypic differences, the species Flavobacterium akiainvivens sp. nov. is proposed to accommodate strain IK-1(T) ( =ATCC BAA-2412(T) =CIP 110358(T)) as the type strain. The description of the genus Flavobacterium is emended to reflect the DNA G+C contents of Flavobacterium akiainvivens IK-1(T) and other species of the genus Flavobacterium described since the original description of the genus.
authors.affiliation: Department of Microbiology, University of Hawai'i at Mânoa, Snyder Hall, 2538 McCarthy Mall, Honolulu, HI 96822, USA.|'Iolani School, 563 Kamoku Street, Honolulu, HI 96826, USA.
authors.forename: Iris
authors.initials: I
authors.lastname: Kuo
authors.affiliation: Department of Microbiology, University of Hawai'i at Mânoa, Snyder Hall, 2538 McCarthy Mall, Honolulu, HI 96822, USA.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Saw
authors.affiliation: Department of Entomology & Center for Comparative Genomics, California Academy of Sciences, 55 Music Concourse Drive, Golden Gate Park, San Francisco, CA 94118, USA.|Center for Conservation Research & Training, Pacific Biosciences Research Center, University of Hawai'i at Mânoa, 3050 Maile Way, Gilmore 406, Honolulu, HI 96822, USA.
authors.forename: Durrell D
authors.initials: DD
authors.lastname: Kapan
authors.affiliation: Department of Oceanography, SOEST, Marine Science Building, University of Hawai'i at Mânoa, 1000 Pope Road, Honolulu, HI 96822, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Christensen
authors.affiliation: Center for Conservation Research & Training, Pacific Biosciences Research Center, University of Hawai'i at Mânoa, 3050 Maile Way, Gilmore 406, Honolulu, HI 96822, USA.
authors.forename: Kenneth Y
authors.initials: KY
authors.lastname: Kaneshiro
authors.affiliation: Department of Microbiology, University of Hawai'i at Mânoa, Snyder Hall, 2538 McCarthy Mall, Honolulu, HI 96822, USA.
authors.forename: Stuart P
authors.initials: SP
authors.lastname: Donachie
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006254:Hawaii; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D032365:Wikstroemia / Q000382:microbiology*; D014934:Wood / Q000382:microbiology*; D024341:Xanthophylls / Q000032:analysis; D065146:Zeaxanthins
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024341:Xanthophylls; D065146:Zeaxanthins