title: Antioxidant activity evaluation of new dosage forms as vehicles for dehydrated vegetables.
abstract: A dehydrated vegetables mixture loaded in four pharmaceutical dosage forms as powder, effervescent granulate, sugar granulate and gumdrops were investigated for their antioxidant capacity using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging capacity assay, oxygen radical absorbance capacity assay and ferric reducing antioxidant potential assay. Total phenolic content of dehydrated vegetables powder mixture was also measured by the Folin-Ciocalteu method, so as to evaluate its contribution to their total antioxidant function. The effect of different temperatures on stability of these systems after 90 days storage was also evaluated. These formulations presented strong antioxidant properties and high phenolic content (279 mg gallic acid equivalent/g of sample) and thus could be potential rich sources of natural antioxidants. Antioxidant properties differed significantly among selected formulations (p < 0.05). Generally, the losses were lower in samples stored under refrigeration. To interpret the antioxidant properties a kinetic approach was performed. Degradation kinetics for the phenolic content and antioxidant capacity followed a zero-order function. Effervescent granulate was the formulation which underwent faster degradation. Contrary, sugar granulate and gumdrops were much more slowly. Time required to halve the initial amount of phenolic compounds was 589 ± 45 days for samples stored at 4 º C, and 312 ± 16 days for samples stored at room temperature. These developed dosage forms are new and innovative approach for vegetable intakes in population with special requirements providing an improvement in the administration of vegetables and fruits.
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Granada, Granada, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Romero-de Soto
authors.forename: Patricia
authors.initials: P
authors.lastname: García-Salas
authors.forename: Salvador
authors.initials: S
authors.lastname: Fernández-Arroyo
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura-Carretero
authors.forename: Francisco
authors.initials: F
authors.lastname: Fernández-Campos
authors.forename: Beatriz
authors.initials: B
authors.lastname: Clares-Naveros
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D004304:Dosage Forms / Q000592:standards*; D004356:Drug Storage; D061353:Food Storage; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology; D005707:Gallic Acid / Q000032:analysis; D007700:Kinetics; D010636:Phenols / Q000032:analysis; D011208:Powders; D013696:Temperature; D014675:Vegetables / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004304:Dosage Forms; D016166:Free Radical Scavengers; D010636:Phenols; D011208:Powders; D005707:Gallic Acid