title: Changes of intestinal tight junctions in rat ileums after partial hepatectomy.
abstract: BACKGROUND
  Increased intestinal permeability and bacterial translocation have been observed after partial hepatectomy (PH).
  
  
  METHODS
  Rats (n = 40) were randomly divided into four groups: control; PH 1d, PH 3d and PH 5d. PH animals underwent two-thirds partial hepatectomy before sacrifice at 1, 3, or 5 days thereafter. The intestinal tight junction (TJ) morphology was observed by transmission electron microscopy. Distribution of TJ protein occludin in lipid raft microdomains was examined using Western blot analysis.
  
  
  RESULTS
  Intestinal TJ structures revealed significant changes at 1 day after PH that had partly recovered by 5 days. During this period, occludin protein decreased significantly in lipid raft microdomains, increasing partially by 5 days.
  
  
  CONCLUSIONS
  PH induced disruption of TJ structures in rat ilea, which was associated with redistribution of occludin protein in lipid raft microdomains.
authors.affiliation: Department of General Surgery, the Affiliated Drum Tower Hospital of Nanjing University Medical College, Nanjing, China.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Qiu
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Tan
authors.forename: X
authors.initials: X
authors.lastname: Yuan
authors.forename: W
authors.initials: W
authors.lastname: Guan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D006498:Hepatectomy / Q000379:methods*; D007082:Ileum / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D021962:Membrane Microdomains / Q000378:metabolism / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D062793:Occludin / Q000378:metabolism; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D019108:Tight Junctions / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D062793:Occludin; C568987:Ocln protein, rat