title: Analytical methods for measuring collagen XIX in human cell cultures, tissue extracts, and biological fluids.
abstract: Type XIX collagen is a minor collagen associated with basement membranes in vascular, neuronal, mesenchymal, and epithelial tissues. We demonstrated that the NC1, C-terminal, domain of collagen XIX inhibits the migration capacities of tumor cells and exerts a strong inhibition of tumor growth. Other basement membrane collagens or derived fragments were measured in biological fluids such as blood and urine of patients and appeared to be useful for diagnosis, prognosis, or treatment monitoring. The aim of this study was to develop and validate methods to measure collagen XIX and its fragments in human cell cultures, tissue extracts, and human biological fluids. For that purpose, we developed real-time PCR, Western blot, and competitive enzyme-linked immunosorbent assays. We demonstrated that the methods developed in this paper are specific for collagen XIX. We showed that it is expressed in human cell cultures, tissue extracts, and various biological fluids. These methods may be used in various human tissue extracts and biological fluids such as serum, amniotic fluid, cord blood, and many other fluids. Collagen XIX or its fragments could constitute new biomarkers for human diseases as well as for diagnosis and/or prognosis.
authors.affiliation: Université de Reims Champagne-Ardenne, FRE CNRS No. 3481, Reims, France.
authors.forename: J B
authors.initials: JB
authors.lastname: Oudart
authors.forename: S
authors.initials: S
authors.lastname: Brassart-Pasco
authors.forename: E
authors.initials: E
authors.lastname: Luczka
authors.forename: A
authors.initials: A
authors.lastname: Dupont-Deshorgue
authors.forename: G
authors.initials: G
authors.lastname: Bellon
authors.forename: S P
authors.initials: SP
authors.lastname: Boudko
authors.forename: H P
authors.initials: HP
authors.lastname: Bächinger
authors.forename: J C
authors.initials: JC
authors.lastname: Monboisse
authors.forename: F X
authors.initials: FX
authors.lastname: Maquart
authors.forename: L
authors.initials: L
authors.lastname: Ramont
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000145:classification* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D012516:Osteosarcoma / Q000737:chemistry / Q000378:metabolism; D014020:Tissue Extracts / Q000737:chemistry*
chemical_list: D014020:Tissue Extracts; D003094:Collagen