title: Induction of annexin by heavy metals and jasmonic acid in Zea mays.
abstract: Plant annexins are Ca(2+)- and phospholipid-binding proteins forming an evolutionary conserved multi-gene family. They are implicated in the regulation of plant growth, development, and stress responses. With the availability of the maize genome sequence information, we identified 12 members of the maize annexin genes. Analysis of protein sequence and gene structure of maize annexins led to their classification into five different orthologous groups. Expression analysis by RT-PCR revealed that these genes are responsive to heavy metals (Ni, Zn, and Cd). The maize annexin genes were also found to be regulated by Ustilago maydis and jasmonic acid. Additionally, the promoter of the maize annexin gene was analyzed for the presence of different stress-responsive cis-elements, such as ABRE, W-box, GCC-box, and G-box. RT-PCR and microarray data show that all 12 maize annexin genes present differential, organ-specific expression patterns in the maize developmental steps. These results indicate that maize annexin genes may play important roles in the adaptation of plants to various environmental stresses.
authors.affiliation: School of Life and Basic Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, People's Republic of China.
authors.forename: Mei-Liang
authors.initials: ML
authors.lastname: Zhou
authors.forename: Xiong-Bang
authors.initials: XB
authors.lastname: Yang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: En-Ze
authors.initials: EZ
authors.lastname: Zhao
authors.forename: Yi-Xiong
authors.initials: YX
authors.lastname: Tang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhu
authors.forename: Ji-Rong
authors.initials: JR
authors.lastname: Shao
authors.forename: Yan-Min
authors.initials: YM
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D017302:Annexins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D019216:Metals, Heavy / Q000633:toxicity*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development
chemical_list: D017302:Annexins; D003517:Cyclopentanes; D019216:Metals, Heavy; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid