title: Electrospun fiber membranes enable proliferation of genetically modified cells.
abstract: Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces.
authors.affiliation: Department of Chemistry, Delaware State University, Dover, DE 19901, USA.
authors.forename: Mandula
authors.initials: M
authors.lastname: Borjigin
authors.forename: Chris
authors.initials: C
authors.lastname: Eskridge
authors.forename: Rohina
authors.initials: R
authors.lastname: Niamat
authors.forename: Bryan
authors.initials: B
authors.lastname: Strouse
authors.forename: Pawel
authors.initials: P
authors.lastname: Bialk
authors.forename: Eric B
authors.initials: EB
authors.lastname: Kmiec
mesh_terms: D001483:Base Sequence; D002152:Calorimetry, Differential Scanning; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005780:Gelatin / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D054709:Lecithins / Q000737:chemistry; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D054709:Lecithins; D008567:Membranes, Artificial; D011091:Polyesters; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C016240:polycaprolactone; D005780:Gelatin; D048271:Chitosan
keywords: PCL-biomaterial blends; cell proliferation; gene editing; miscibility; nanofibers