title: Problem-solving test: pyrosequencing.
abstract: Terms to be familiar with before you start to solve the test: Maxam-Gilbert sequencing, Sanger sequencing, gel electrophoresis, DNA synthesis reaction, polymerase chain reaction, template, primer, DNA polymerase, deoxyribonucleoside triphosphates, orthophosphate, pyrophosphate, nucleoside monophosphates, luminescence, acid anhydride bond, phosphodiester bond, mismatch, proofreading, 5'→3' exonuclease.
authors.affiliation: Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi
mesh_terms: D004521:Educational Measurement; D017422:Sequence Analysis, DNA / Q000379:methods*; D011795:Surveys and Questionnaires; D013696:Temperature