title: Identification and expression of stressosomal proteins in Mycobacterium marinum under various growth and stress conditions.
abstract: Like other bacteria, Mycobacterium spp. have developed different strategies in response to environmental changes such as nutrient limitations and other different stress situations. We have identified candidate genes (rsb genes) from Mycobacterium marinum involved in the regulation of the activity of the alternative sigma factor, σ(F) . This is a homolog of the master regulator of general stress response, σ(B) , and the sporulation-specific sigma factor, σ(F) , in Bacillus subtilis. The organization of these genes in M. marinum and B. subtilis is similar. Transcriptome and qRT-PCR data show that these genes are indeed expressed in M. marinum and that the levels of expression vary with growth phase and exposure to stress. In particular, cold stress caused a significant rise in the expression of all identified rsb and sigF genes. We discuss these data in relation to what is currently known for other Mycobacterium spp.
authors.affiliation: Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, SE-751 24 Uppsala, Sweden.
authors.forename: B M Fredrik
authors.initials: BM
authors.lastname: Pettersson
authors.forename: Ram G
authors.initials: RG
authors.lastname: Nitharwal
authors.forename: Sarbashis
authors.initials: S
authors.lastname: Das
authors.forename: Krishna P R
authors.initials: KP
authors.lastname: Behra
authors.forename: Evgen
authors.initials: E
authors.lastname: Benedik
authors.forename: Uma T
authors.initials: UT
authors.lastname: Arasu
authors.forename: Nurul M
authors.initials: NM
authors.lastname: Islam
authors.forename: Santanu
authors.initials: S
authors.lastname: Dasgupta
authors.forename: Alok
authors.initials: A
authors.lastname: Bhattacharya
authors.forename: Leif A
authors.initials: LA
authors.lastname: Kirsebom
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D019910:Mycobacterium marinum / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins