title: Enzymatic production of L-alanyl-L-glutamine by recombinant E. coli expressing α-amino acid ester acyltransferase from Sphingobacterium siyangensis.
abstract: An enzymatic production method for synthesizing L-alanyl-L-glutamine (Ala-Gln) from L-alanine methyl ester hydrochloride (AlaOMe) and L-glutamine (Gln) was developed in this study. The cultivation conditions for an Escherichia coli strain overexpressing α-amino acid ester acyltransferase from Sphingobacterium siyangensis AJ 2458 (SAET) and reaction conditions for Ala-Gln production were optimized. A high cell density culture broth prepared by fed-batch cultivation showed 440 units/mL of Ala-Gln-producing activity. In addition, an Ala-Gln-producing reaction using intact E. coli cells overexpressing SAET under optimum conditions was conducted. A total Ala-Gln yield of 69.7 g/L was produced in 40 min. The molar yield was 67% against both AlaOMe and Gln.
authors.affiliation: Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., Inc.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Hirao
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Mihara
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kira
authors.forename: Isao
authors.initials: I
authors.lastname: Abe
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yokozeki
mesh_terms: D051046:Amino-Acid N-Acetyltransferase; D046508:Culture Techniques; D004274:DNA, Recombinant / Q000235:genetics*; D004151:Dipeptides / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D007221:Industry; D045249:Sphingobacterium / Q000201:enzymology* / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004151:Dipeptides; D051046:Amino-Acid N-Acetyltransferase; C054122:alanylglutamine