title: Engineering of therapeutic and diagnostic O-glycans on recombinant mucin-type immunoglobulin fusion proteins expressed in CHO cells.
abstract: Metabolic engineering of mammalian cells for optimized glycosylation is usually done to improve activity and the pharmacokinetic features of glycoprotein therapeutics. The field is mainly focused around engineering of N-glycans. We have created a platform in which recombinant mucin-type immunoglobulin fusion proteins are used as scaffolds for multivalent expression of O-glycans with diagnostic or therapeutic potential. The methods used to make stable CHO cell lines secreting a mucin-type fusion protein with blood group A or B determinants following expression of up to five different cDNAs are described.
authors.affiliation: AbSorber AB, Stockholm, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Lindberg
authors.forename: Jining
authors.initials: J
authors.lastname: Liu
authors.forename: Jan
authors.initials: J
authors.lastname: Holgersson
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D015870:Gene Expression; D006031:Glycosylation; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics; D009077:Mucins / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D015202:Protein Engineering / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D007136:Immunoglobulins; D009077:Mucins; D011993:Recombinant Fusion Proteins