title: Analysis of mitochondrial gene expression.
abstract: Dictyostelium provides a well-established model system for the study of mitochondrial biology and disease. A complete mitochondrial transcription and RNA-processing map has been generated, while the start site for transcription and the responsible RNA polymerase have also been identified, as have the major cotranscriptional cleavage sites that generate the mature mitochondrial RNA molecules. Here we describe the methods deployed to study mitochondrial gene transcription and RNA processing in Dictyostelium.
authors.affiliation: Department of Microbiology, La Trobe University, Bundoora, Australia.
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Accari
authors.forename: Sam
authors.initials: S
authors.lastname: Manna
authors.forename: Paul R
authors.initials: PR
authors.lastname: Fisher
authors.forename: Christian
authors.initials: C
authors.lastname: Barth
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D002458:Cell Fractionation / Q000379:methods; D002874:Chromosome Mapping / Q000379:methods; D003851:Deoxyribonucleases / Q000737:chemistry; D004023:Dictyostelium / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D050259:Genes, Mitochondrial; D017125:Genes, Protozoan; D008928:Mitochondria / Q000235:genetics; D011401:Promoter Regions, Genetic; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D012329:RNA, Bacterial; D016053:RNA, Protozoan; D003851:Deoxyribonucleases