title: [HPLC determination of hydroxysafflor yellow A in Mongolian medicine Dedu Honghuaqiwei pill].
abstract: OBJECTIVE
  To establish a method for the determination of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.
  
  
  METHODS
  The determination was performed by HPLC method on Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column at 403 nm using methanol-acetonitrile-0.7% phosphoric acid-water (26: 2: 72) as mobile phase. The column temperature was 30 degrees C and the flow rate was 1.0 mL x min(-1).
  
  
  RESULTS
  The linear rang of hydroxysafflor yellow A was 0.068-0.408 microg and the recovery was 97.66%.
  
  
  CONCLUSIONS
  The result is accurate with good resolution, and the established method can be applied to determine the content of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.
authors.affiliation: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao 028000, China. changlian826@126.com
authors.forename: Chang-Lian
authors.initials: CL
authors.lastname: Chen
authors.forename: Si-Le
authors.initials: SL
authors.lastname: Hu
mesh_terms: D002599:Chalcone / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D056785:Medicine, Mongolian Traditional; D011809:Quinones / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D011809:Quinones; C085278:hydroxysafflor yellow A; D002599:Chalcone