title: Functionalized magnetic nanoparticles for the detection and quantitative analysis of cell surface antigen.
abstract: Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis.
authors.affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Hezar Jerib Street, Isfahan, Iran. shahbazi@med.mui.ac.ir
authors.forename: Daryoush
authors.initials: D
authors.lastname: Shahbazi-Gahrouei
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdolahi
authors.forename: Sayyed Hamid
authors.initials: SH
authors.lastname: Zarkesh-Esfahani
authors.forename: Sophie
authors.initials: S
authors.lastname: Laurent
authors.forename: Corine
authors.initials: C
authors.lastname: Sermeus
authors.forename: Cordula
authors.initials: C
authors.lastname: Gruettner
mesh_terms: D000912:Antibodies, Neoplasm / Q000737:chemistry* / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D058185:Magnetite Nanoparticles