title: Antimicrobial activity and hydrophobicity of edible whey protein isolate films formulated with nisin and/or glucose oxidase.
abstract: The use of edible antimicrobial films has been reported as a means to improve food shelf life through gradual releasing of antimicrobial compounds on the food surface. This work reports the study on the incorporation of 2 antimicrobial agents, nisin (N), and/or glucose oxidase (GO), into the matrix of Whey protein isolate (WPI) films at pH 5.5 and 8.5. The antimicrobial activity of the edible films was evaluated against Listeria innocua (ATCC 33090), Brochothrix thermosphacta (NCIB10018), Escherichia coli (JMP101), and Enterococcus faecalis (MXVK22). In addition, the antimicrobial activity was related to the hydrophobicity and water solubility of the WPI films. The greatest antibacterial activity was observed in WPI films containing only GO. The combined addition of N and GO resulted in films with lower antimicrobial activity than films with N or GO alone. In most cases, a pH effect was observed as greater antimicrobial response at pH 5.5 as well as higher film matrix hydrophobicity. WPI films supplemented with GO can be used in coating systems suitable for food preservation.
authors.affiliation: Departamento de Biotecnología and Química, Univ. Autónoma Metropolitana Unidad Iztapalapa, Av. San Rafael Atlixco nr 186, Col. Vicentina, Iztapalapa, 09340, México.
authors.forename: María M
authors.initials: MM
authors.lastname: Murillo-Martínez
authors.forename: Salvador R
authors.initials: SR
authors.lastname: Tello-Solís
authors.forename: Miguel A
authors.initials: MA
authors.lastname: García-Sánchez
authors.forename: Edith
authors.initials: E
authors.lastname: Ponce-Alquicira
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects / Q000254:growth & development; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods*; D005949:Glucose Oxidase / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D008087:Listeria / Q000187:drug effects / Q000254:growth & development; D008460:Meat / Q000382:microbiology; D008894:Milk Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009561:Nisin / Q000494:pharmacology*; D014675:Vegetables / Q000382:microbiology; D000067816:Whey Proteins
chemical_list: D000890:Anti-Infective Agents; D008894:Milk Proteins; D000067816:Whey Proteins; D009561:Nisin; D005949:Glucose Oxidase