title: Simultaneous saccharification and fermentation of fungal pretreated cornstalk for hydrogen production using Thermoanaerobacterium thermosaccharolyticum W16.
abstract: In this research, environmentally friendly fungal pretreatment was first adopted for deconstruction of cornstalk. Then the fungal-pretreated cornstalk was employed to produce hydrogen in simultaneous saccharification and fermentation (SSF) using crude enzyme from Trichoderma viride and Thermoanaerobacterium thermosaccharolyticum W16. The influence of various factors including substrate concentration, initial pH, and enzyme loading on hydrogen production were evaluated. The highest hydrogen yield of 89.3 ml/g-cornstalk was obtained with an initial pH 6.5, 0.75% substrate concentration, and 34 FPU/g cellulose. Compared the result with SSF of physical or chemical pretreated lignocellulosic materials, this research suggested an economic and efficient way for hydrogen production from lignocellulosic biomass.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Cao
authors.forename: Ai-Jie
authors.initials: AJ
authors.lastname: Wang
authors.forename: Wan-Qian
authors.initials: WQ
authors.lastname: Guo
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Ren
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
mesh_terms: D019149:Bioreactors; D044602:Cellulases / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D018547:Plant Stems / Q000737:chemistry*; D046950:Thermoanaerobacterium / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology; D003313:Zea mays / Q000737:chemistry*
chemical_list: C036909:lignocellulose; D006859:Hydrogen; D008031:Lignin; D044602:Cellulases
keywords: Fungal-pretreated cornstalk; Hydrogen production; SSF; T. thermosaccharolyticum W16