title: [Effects of tongxinluo on angiogenesis and the volume of blood perfusion in ischemic stroke rats].
abstract: OBJECTIVE
  To observe the effects of Tongxinluo (TXL) on angiogenesis and the volume of blood perfusion in ischemic stroke rats.
  
  
  METHODS
  The model of middle cerebral artery occlusion (MCAO) was established using craniotomy ligation of the middle cerebral artery on one side. After screening, the male SD rats were randomly divided into the sham-operation group, the model group, the large dose TXL group, the middle dose TXL group, the low dose TXL group, and the Nimodipine group. The expression of microvascular density (MVD, CD31) of the MCAO rats was detected using immunohistochemical assay after 14 days of medication. The microvascular morphology and the volume of blood perfusion in the brain tissue were observed under laser scanning confocal microscope (LSCM).
  
  
  RESULTS
  The positive CD31 expression was intense with significant coloring in the large dose TXL group, the middle dose TXL group, and the Nimodipine group, better than that of the model group. The blood perfusion volume in the ischemic brain cortex could be promoted in the large dose TXL group, the middle dose TXL group, and the Nimodipine group (P<0.01, P<0.05). The optimal effects were shown in the large dose TXL group (P<0.01).
  
  
  CONCLUSIONS
  TXL significantly increased the MVD of the ischemic brain tissue, promoted the post-ischemic angiogenesis, and increased the volume of blood perfusion of ischemic brain tissue, playing certain blood flow compensatory roles.
authors.affiliation: Yiling Medical Research Institute of Hebei Province, Key Laboratory of State Administration of Traditional Chinese Medicine (Cardio-/Cerebrovascular Collateral Diseases), Shijiazhuang 050035. changliping08@163.com
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Chang
authors.forename: Cong
authors.initials: C
authors.lastname: Wei
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Jia
mesh_terms: D000818:Animals; D002545:Brain Ischemia; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006464:Hemoperfusion; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009553:Nimodipine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; C513941:tongxinluo; D009553:Nimodipine