title: Biocompatible nanostructured magnesium oxide-chitosan platform for genosensing application.
abstract: A novel organic-inorganic platform comprising of chitosan (CH) modified nanostructured magnesium oxide (nanoMgO) has been electrophoretically deposited on the indium-tin-oxide (ITO) substrate. The single stranded probe DNA (ssDNA) sequence of Vibrio cholerae has been covalently functionalized onto CH-nanoMgO/ITO surface. The cytotoxicity assay of nanoMgO particles, examined using human intestinal cell line (INT 407), reveals no significant cytotoxicity at the given doses in the range of 50-350 μg/mL. The X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and various microscopic techniques have been employed for the structural and morphological analysis of the fabricated electrodes. The electrochemical response studies of ssDNA and fragmented genomic DNA hybridized electrode (dsGDNA/CH-nanoMgO/ITO) have been carried out using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The dsGDNA/CH-nanoMgO/ITO bioelectrode exhibits a linear response in the range 100-500 ng/μL with improved sensitivity of 36.72 nA/ng/cm(2), faster response time of 3s and high stability of 3-4 months under refrigerated condition. The lower detection limit of fabricated genosensor has been estimated as 35.20 ng/μL and it shows good reproducibility/repeatability.
authors.affiliation: Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Patel
authors.forename: Md Azahar
authors.initials: MA
authors.lastname: Ali
authors.forename: Md
authors.initials: M
authors.lastname: Zafaryab
authors.forename: Ved Varun
authors.initials: VV
authors.lastname: Agrawal
authors.forename: M Moshahid Alam
authors.initials: MM
authors.lastname: Rizvi
authors.forename: Z A
authors.initials: ZA
authors.lastname: Ansari
authors.forename: S G
authors.initials: SG
authors.lastname: Ansari
authors.forename: Bansi D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004563:Electrochemistry; D006801:Humans; D007204:Indium / Q000737:chemistry; D057230:Limit of Detection; D008277:Magnesium Oxide / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds / Q000737:chemistry; D014734:Vibrio cholerae / Q000302:isolation & purification* / Q000472:pathogenicity; D014961:X-Ray Diffraction
chemical_list: D004277:DNA, Single-Stranded; D017971:Tin Compounds; D007204:Indium; D008277:Magnesium Oxide; D048271:Chitosan; C045358:stannic oxide