title: Establishment of a central post-stroke pain model using global cerebral ischaemic mice.
abstract: OBJECTIVE
  Stroke is the leading cause of disability in the world. Central post-stroke pain (CPSP), an intractable secondary disease, is a serious problem that occurs following cerebral stroke. However, the detailed mechanisms underlying CPSP and standard treatments for it are not well established. Therefore, we examined the nociceptive threshold and alterations in the current stimulus threshold of primary afferent neurons in bilateral carotid artery occlusion (BCAO) mice.
  
  
  METHODS
  Male ddY mice were subjected to 30 min of BCAO. The development of mechanical and thermal hyperalgesia and changes in current stimulus threshold in the hind paws were measured after BCAO using the von Frey test, plantar test and a Neurometer, respectively.
  
  
  RESULTS
  The threshold for mechanical and thermal hyperalgesia in both hind paws was significantly decreased on day 3 after BCAO as compared with pre-BCAO treatment. Furthermore, the sensitivity of C and Aβ fibres (at stimulation of 5 and 2000 Hz, respectively) was increased on day 3 after BCAO as compared with pre-BCAO treatment, while that of Aδ fibres was not altered.
  
  
  CONCLUSIONS
  Our data show the development of bilateral hyperalgesia in this model. Potentially, C and Aβ fibre-specific hypersensitization after stroke may have contributed to these symptoms.
authors.affiliation: Department of Clinical Pharmacy, Kobe Gakuin University, School of Pharmaceutical Sciences, Kobe, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tamiya
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Shinichi
authors.initials: S
authors.lastname: Harada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakamoto
authors.forename: Shogo
authors.initials: S
authors.lastname: Tokuyama
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D002339:Carotid Arteries / Q000601:surgery; D002540:Cerebral Cortex / Q000098:blood supply* / Q000378:metabolism; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000009:adverse effects; D006614:Hindlimb; D006358:Hot Temperature / Q000009:adverse effects; D006930:Hyperalgesia / Q000209:etiology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009413:Nerve Fibers, Myelinated / Q000378:metabolism; D036421:Nerve Fibers, Unmyelinated / Q000378:metabolism; D009475:Neurons, Afferent / Q000378:metabolism; D017288:Pain Threshold; D010148:Intractable Pain / Q000209:etiology* / Q000378:metabolism; D010812:Physical Stimulation / Q000009:adverse effects; D020521:Stroke / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D013525:Surgical Instruments