title: Inhaled nitric oxide decreases the bacterial load in a rat model of Pseudomonas aeruginosa pneumonia.
abstract: Gaseous nitric oxide (NO) is bactericidal in vitro. However whether and how it can be used for the treatment of bacterial lung infections in patients with cystic fibrosis is unclear. Here we assessed the bactericidal effect of intermittently inhaled 160 ppm NO for 30 min every 4 h in a Pseudomonas aeruginosa pneumonia model in rats. NO significantly reduced P. aeruginosa colony count in rat lungs but did not affect neutrophil myeloperoxidase function methemoglobin percentage nor plasma nitrite/nitrate levels. This regimen warrants exploration in infected patients with cystic fibrosis.
authors.affiliation: Division of Respiratory Medicine, University of British Columbia, Vancouver, BC, Canada; Division of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada. Electronic address: miller42@mail.ubc.ca.
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Miller
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Hergott
authors.forename: Marta
authors.initials: M
authors.lastname: Rohan
authors.forename: Kyle
authors.initials: K
authors.lastname: Arsenault-Mehta
authors.forename: Gerd
authors.initials: G
authors.lastname: Döring
authors.forename: Sanjay
authors.initials: S
authors.lastname: Mehta
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D058491:Bacterial Load / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D009569:Nitric Oxide / Q000008:administration & dosage / Q000494:pharmacology*; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009569:Nitric Oxide
keywords: Cystic fibrosis; Inhalation; Myeloperoxidase; Nitric oxide; P. aeruginosa pneumonia