title: Distribution characteristics of DNA vaccine encoded with glycoprotein C from Anatid herpesvirus 1 with chitosan and liposome as deliver carrier in ducks.
abstract: BACKGROUND
  A eukaryotic expression plasmid encoding glycoprotein C (gC) of Anatid herpesvirus 1 (AnHV-1) (pcDNA3.1-gC) was constructed and validated. The tissue distribution of chitosan/DNA complexes, liposome/DNA complexes and pcDNA3.1-gC alone were evaluated using a quantitative real-time PCR based TaqMan™ probe following intramuscular administration in ducklings.
  
  
  RESULTS
  Compared with pcDNA3.1-gC alone, liposomes universally increased the plasmid DNA copy number at the injection sites, liver, spleen, heart, brain, bursa of Fabricius, and especially in the enteron (esophagus, duodenum, rectum, and cecum). Chitosan also universally increased the plasmid DNA copy number at the injection sites, liver, spleen, heart, brain and esophagus. Compared with lipoplex-gC, higher chitosan-gC plasmid DNA copy numbers were detected at the injection sites, liver, spleen, heart, brain and esophagus. In contrast, compared with lipoplex-gC, lower copy numbers of chitosan-gC plasmid DNA were detected in the duodenum, rectum and cecum.
  
  
  CONCLUSIONS
  The results of this study demonstrated that chitosan and liposomes mediated rapid and extensive plasmid distribution in duck tissues, with low levels maintained from 1 d after DNA vaccination.
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, China.
authors.forename: Kunfeng
authors.initials: K
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Jiang
authors.forename: Anchun
authors.initials: A
authors.lastname: Cheng
authors.forename: Mingshu
authors.initials: M
authors.lastname: Wang
authors.forename: Dekang
authors.initials: D
authors.lastname: Zhu
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.forename: Shun
authors.initials: S
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
mesh_terms: D018138:Alphaherpesvirinae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D004372:Ducks; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D008081:Liposomes / Q000737:chemistry; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D014018:Tissue Distribution; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*; D014764:Viral Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D048271:Chitosan