title: [Regularity of changes in chlorogenic acids in Lonicera japonica extracts by HPLC-DAD-ESI-MS/MS].
abstract: OBJECTIVE
  To analyze chlorogenic acids contained in Lonicera japonica water extracts, and to investigate the regularity of changes in chlorogenic acids during the water extraction process.
  
  
  METHODS
  Agilent Zorbax SB-C18 analytical column (4.6 mm x 250 mm, 5 microm) was eluted with 0.1% formic acid (A)-acetonitrile (B) as the mobile phase at a flow rate of 0.5 mL x min(-1). The detection wavelength was 327 nm and the column temperature was 30 degrees C. Negative MS(n) mode was adopted in mass spectrum for ananlyzing the 22 samples of L. japonica water extracts.
  
  
  RESULTS
  Caffeic acid and six organic acids were accurately identified from the water extracts. During the extraction, the contents of chlorogenic acid and 3,5-dicaffeoylquinic acid became stable or gradually decreased after reaching the highest value. The contents of other components had long been increasing, but with a decreasing rate of change.
  
  
  CONCLUSIONS
  This study provides basis for improving the production process of traditional Chinese medicine preparations containing L. japonica.
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China. dazhangqian@126.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Zhagn
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Sui
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Shi
authors.forename: Yan-Jiang
authors.initials: YJ
authors.lastname: Qiao
authors.forename: Jian-Qiu
authors.initials: JQ
authors.lastname: Lu
mesh_terms: D002726:Chlorogenic Acid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031239:Lonicera / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid