title: [Comparison of anti-inflammatory effect and analysis of astilbin red and white transverse section Smilax glabra in 28 collection sites].
abstract: OBJECTIVE
  Astilbin in 28 Smilax glabra (red and white cross-section) from different sources was determined by HPLC. Pharmacodynamics and component of S. glabra was investigated through inflammation experiment (penetration type).
  
  
  METHODS
  The analysis was performed on a Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0. 1% acetic acid aqueous (21: 79) at a flow rate of 1.0 mL x min(-1). The detection wavelength was 291 nm, and the column temperature was 25 degrees C. Anti-inflammatory effect was compared from two type cross-section of Smilax glabra in capillary permeability experiment.
  
  
  RESULTS
  Linear correlation was good in the range of 0.003 379-4.004 microg, and the average recoveries were 100.1%, 101.9%, 99.3%, respectively. The content of astilbin in white and red transverse section were 0.19%-2.46% and 2.10%-5.92%, respectively. Anti-inflammatory efficiency of sectioned red and white were were 21% and 32%, respectively.
  
  
  CONCLUSIONS
  Astilbin content and anti-inflammatory effect is significantly different between red and white transverse section of S. glabra, the content of astilbin is not positively related with anti-inflammatory effect.
authors.affiliation: Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China. 234208617@qq.com
authors.forename: Xi-Cheng
authors.initials: XC
authors.lastname: He
authors.forename: Qin-Wen
authors.initials: QW
authors.lastname: Shun
authors.forename: Li-Sha
authors.initials: LS
authors.lastname: Dong
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Ge
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Nong
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Shen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology; D002681:China; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D044948:Flavonols / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D051379:Mice; D010539:Permeability; D032281:Smilax / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D044948:Flavonols; C099069:astilbin