title: A reagent-free tubular biofilm reactor for on-line determination of biochemical oxygen demand.
abstract: We reported a reagent-free tubular biofilm reactor (BFR) based analytical system for rapid online biochemical oxygen demand (BOD) determination. The BFR was cultivated using microbial seeds from activated sludge. It only needs tap water to operate and does not require any chemical reagent. The analytical performance of this reagent-free BFR system was found to be equal to or better than the BFR system operated using phosphate buffer saline (PBS) and high purity deionized water. The system can readily achieve a limit of detection of 0.25 mg O2 L(-1), possessing superior reproducibility, and long-term operational and storage stability. More importantly, we confirmed for the first time that the BFR system is capable of tolerating common toxicants found in wastewaters, such as 3,5-dichlorophenol and Zn(II), Cr(VI), Cd(II), Cu(II), Pb(II), Mn(II) and Ni(II), enabling the method to be applied to a wide range of wastewaters. The sloughing and clogging are the important attributes affecting the operational stability, hence, the reliability of most online wastewater monitoring systems, which can be effectively avoided, benefiting from the tubular geometry of the reactor and high flow rate conditions. These advantages, coupled with simplicity in device, convenience in operation and minimal maintenance, make such a reagent-free BFR analytical system promising for practical BOD online determination.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Liu
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhao
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.forename: Jianbo
authors.initials: J
authors.lastname: Jia
authors.forename: Limin
authors.initials: L
authors.lastname: Zhao
authors.forename: Daming
authors.initials: D
authors.lastname: Yong
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
mesh_terms: D018441:Biofilms; D019149:Bioreactors; D007202:Indicators and Reagents; D010100:Oxygen / Q000737:chemistry; D010101:Oxygen Consumption; D012965:Sodium Chloride / Q000737:chemistry; D062065:Wastewater / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D010100:Oxygen; D012965:Sodium Chloride; D062065:Wastewater; D014867:Water