title: [Leptospira spp. and Coxiella burnetii infections occuring in Radomskie District in people of selected professional groups].
abstract: BACKGROUND
  Leptospirosis and Q fever are a zoonotic diseases with global occurring.
  
  
  OBJECTIVE
  The aim of this study was to determine the prevalence of Leptospira spp. and C. burnetii in humans, who have contacts with infected animals or are exposed to an environment potentially contaminated with these bacteria. MATERIAL AND METHODS. Blood serum samples originating from 177 veterinarians and farmers and 134 garbage collectors (blood samples) were examined. Control group consisted 43 blood samples derived from blood donors. For the detection of specific IgM and IgG antibodies of Leptospira spp., enzyme-linked immunosorbent assay (ELISA) was used. Indirect immunofluorescence method (IFA) was used for detection of specific IgG C. burnetii antibodies. DNA of Leptospira spp. and C. burnetii was detected by PCR method with appropriate pairs of primers.
  
  
  RESULTS
  Specific IgG C. burnetii antibodies of phase II were detected in sera of 4.4% of the farmers and veterinarians, and in 12% of garbage men. Antibodies in blood donors was not found. Antibodies of Leptospira spp. were present in the serum of 23.6% of farmers and veterinarians, 26.2% of garbage men and 14% of blood donors. C. burnetii DNA was detected in one sample derived from the veterinarian (1.1%). Leptospira spp. DNA was not detected in tested material. Blood samples from farmers, veterinarians and garbage collectors showed the higher prevalence of antibodies of Leptospira spp. and C. burnetii as compared to the control group (blood donors).
  
  
  CONCLUSIONS
  Beside farmers and veterinarians, garbage collectors should be consider as high risk group of contracting leptospirosis and Q fever. Both leptospirosis as well as Q fever should be considered in the differential diagnosis in humans with animals and animals' material contact when they reveal flu-like symptoms.
authors.affiliation: Samodzielna Pracownia Riketsji, Chlamydii i Kretków Odzwierzecych, Narodowy Instytut Zdrowia Publicznego-Państwowy Zakład Higieny w Warszawie. bfiecek@pzh.gov.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Fiecek
authors.forename: Aneta
authors.initials: A
authors.lastname: Grochowalska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Chmielewski
authors.forename: Stanisława
authors.initials: S
authors.lastname: Tylewska-Wierzbanowska
mesh_terms: D000328:Adult; D000383:Agriculture; D000818:Animals; D000829:Animals, Domestic; D001782:Blood Donors; D016997:Coxiella burnetii / Q000302:isolation & purification; D005260:Female; D005735:Garbage; D006801:Humans; D007919:Leptospira / Q000302:isolation & purification; D007922:Leptospirosis / Q000453:epidemiology*; D008297:Male; D009784:Occupational Diseases / Q000453:epidemiology*; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D011778:Q Fever / Q000175:diagnosis / Q000453:epidemiology*; D018634:Veterinarians; D015047:Zoonoses / Q000453:epidemiology* / Q000382:microbiology