title: [Impact of BKCa channel in prostate smooth muscle cells on the membrane potential in rats with chronic abacterial prostatitis].
abstract: OBJECTIVE
  To investigate the impact of the BKCa channel in prostate smooth muscle cells (PSMCs) on the membrane potential in SD rats with chronic abacterial prostatitis (CAP).
  
  
  METHODS
  CAP models were established in 20 SD rats by castration and injection of 17 beta-estrogen, and another 20 were taken as normal controls. PSMCs were cultured and purified in vitro, and treated with DiBAC4, followed by quantitative observations on the dynamic changes of the cell membrane potential by laser confocal microscopy.
  
  
  RESULTS
  The extracellular calcium ion concentration ([Ca2+]o) was increased and the BKCa channel was activated, which induced the hyperpolarization of the PSMC membrane in both the CAP models and normal control rats. This effect was weakened with Iberiotoxin (IbTX), a specific blocker of the BKCa channel, but the amplitude of the hyperpolarization was obviously lower in the CAP than in the control group. The DiBAC4 fluorescence intensity induced by hyperpolarization was 18.78 +/- 2.92 in the former and 38.85 +/- 7.10 in the latter (P < 0.05), while that induced by IbTX was 1.61 +/- 0.46 and 6.12 +/- 1.32 (P < 0.05), respectively.
  
  
  CONCLUSIONS
  Significant decrease of BKCa-mediated hyperpolarization in the CAP model can reduce its abilities of regulating the membrane potential and suppressing the excessive contraction of PSMCs, which may result in pelvic pain syndrome and lower urinary tract symptoms.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China. zhenzhang1987@163.com
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chao-Zhao
authors.initials: CZ
authors.lastname: Liang
authors.forename: Xian-Sheng
authors.initials: XS
authors.lastname: Zhang
authors.forename: Zong-Yao
authors.initials: ZY
authors.lastname: Hao
authors.forename: Song
authors.initials: S
authors.lastname: Fan
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D008297:Male; D008564:Membrane Potentials; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism*; D011467:Prostate / Q000166:cytology; D011472:Prostatitis / Q000378:metabolism* / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C494842:Kcnma1 protein, rat; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D015221:Potassium Channels