title: Human liver bioengineering using a whole liver decellularized bioscaffold.
abstract: As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Baptista
authors.forename: Dipen
authors.initials: D
authors.lastname: Vyas
authors.forename: Emma
authors.initials: E
authors.lastname: Moran
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Shay
authors.initials: S
authors.lastname: Soker
mesh_terms: D000818:Animals; D019149:Bioreactors; D002469:Cell Separation / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology; D005289:Ferrets; D006801:Humans; D008099:Liver / Q000166:cytology / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds