title: Rapid monitoring of heat-accelerated reactions in vegetable oils using direct analysis in real time ionization coupled with high resolution mass spectrometry.
abstract: Transmission-mode direct analysis in real time ionization coupled with high resolution mass spectrometry (TM-DART-HRMS) was used to monitor chemical changes in various vegetable oils (olive, rapeseed, soybean and sunflower oil) during their thermally-induced oxidation. This novel instrumental approach enabled rapid fingerprinting of examined samples and detection of numerous sample components, such as triacylglycerols (TAGs), phytosterols, free fatty acids (FFA), and their respective oxidation products. Mass spectra obtained from DART were processed with the use of principal component analysis (PCA) in order to assess the compositional differences between heated and non-heated samples. Good correlation was observed between the normalized intensities of the pre-selected ion corresponding to mono-oxidized TAG and 'classic' criterion represented by the levels of TAG polymers determined by high performance-size exclusion chromatography with refractometric detection (HP-SEC-RID).
authors.affiliation: Institute of Chemical Technology Prague, Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, Technicka 5, 166 28 Prague 6, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Vaclavik
authors.forename: Beverly
authors.initials: B
authors.lastname: Belkova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Reblova
authors.forename: Katerina
authors.initials: K
authors.lastname: Riddellova
authors.forename: Jana
authors.initials: J
authors.lastname: Hajslova
mesh_terms: D005227:Fatty Acids / Q000737:chemistry; D006358:Hot Temperature; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010084:Oxidation-Reduction; D010840:Phytosterols / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D010840:Phytosterols; D010938:Plant Oils