title: Identification of the differentially expressed genes in SP2/0 myeloma cells from Balb/c mice infected with Trichinella spiralis.
abstract: To study the molecular mechanism of suppressed growth caused by Trichinella spiralis, an SP2/0 myeloma model was established using parasite-infected Balb/c mice. Suppression subtractive hybridization (SSH) was then utilized to identify differentially expressed genes between tumor cells from the infected and non-infected mice. On the 11th day after infection, 2 × 10(6) SP2/0 myeloma cells were subcutaneously inoculated into 6-8 week old female Balb/c mice in both the experimental and control groups. Twenty-eight days after tumor cell inoculation, the mice were euthanized and the sizes and weights of the tumors were measured. Messenger RNA was isolated and used to perform SSH. Putative differentially expressed genes were identified, sequenced and analyzed by BLASTn. Among the sequences detected which ranged in size between 180 and 850 bp, genes encoding RpL41, NKTR, Rbbp4 and ANXA2 were enriched and considered possible proteins involved in tumor growth inhibition.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Bolin
authors.initials: B
authors.lastname: Deng
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Boqi
authors.initials: B
authors.lastname: Cheng
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Ren
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhang
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006790:Host-Parasite Interactions; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D009194:Myeloma Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D066251:Subtractive Hybridization Techniques; D017160:Trichinella spiralis / Q000502:physiology*; D014235:Trichinellosis / Q000235:genetics*
chemical_list: D019496:Cancer Vaccines; D009194:Myeloma Proteins; D012333:RNA, Messenger