title: Effects of β-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros.
abstract: Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of β-glucan from C. versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in β-glucan-treated macrophages using RT-PCR, western blotting, and IP assay. The role of Ikaros in regulating phagocytic effects of CVG was also determined using Ikaros dominant negative isoform cells. This study suggests that CK2/Ikaros are positive regulators and novel signaling pathway involved in phagocytosis and contributes to elucidating the mechanism underlying phagocytic activity induced by β-glucan.
authors.affiliation: Department of Life Science, Gachon University, Seongnam 461-701, Republic of Korea.
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Koo
authors.forename: Sulkyung
authors.initials: S
authors.lastname: Park
authors.forename: Jung Dae
authors.initials: JD
authors.lastname: Lim
authors.forename: Ye-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Taeseong
authors.initials: T
authors.lastname: Kim
authors.forename: Seung
authors.initials: S
authors.lastname: Namkoong
authors.forename: Ki-Hyo
authors.initials: KH
authors.lastname: Jang
authors.forename: Suhkneung
authors.initials: S
authors.lastname: Pyo
authors.forename: Seon-A
authors.initials: SA
authors.lastname: Jang
authors.forename: Eun-Hwa
authors.initials: EH
authors.lastname: Sohn
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D051740:Ikaros Transcription Factor / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C497238:Zfpn1a1 protein, mouse; D047071:beta-Glucans; D051740:Ikaros Transcription Factor; D051057:Proto-Oncogene Proteins c-akt