title: CD99 and HLA-II immunostaining in breast cancer tissue and their correlation with lymph node metastasis.
abstract: In an attempt to better unfold the antitumor immune response and invasion strategies perused by tumor cells, markers such as CD99 and HLA-II have been stained in breast tumors, some of them turned out to be important for prognosis and its outcome. CD99 is involved in the intracellular transport of HLA-II proteins. The expression of HLA-II and CD99 molecules has been demonstrated in a broader range of neoplastic tissues, including some epithelial tumors. In the present work, we stained CD99 and HLA-II in breast malignant and non-malignant tissues sections obtained from biopsies resected surgically from 80 Tunisian women. Data implied that CD99 marks malignant tissue significantly as compared to non-malignant breast tissue. HLA-II staining allowed determining the correlation between breast cancer and HLA-II with cytoplasmic localization. CD99 and HLA-II immunostaining was also examined in correlation with two of the most important breast cancer prognostication in routine clinical practice, the lymph node stage and the histological assessment. Results let suggest that CD99(+)HLA-II(-) is a marker of worst prognostic since this phenotype is strongly linked to lymph node metastasis in breast cancer.
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, Tunis, Tunisia.
authors.forename: Amal
authors.initials: A
authors.lastname: Baccar
authors.forename: Imen
authors.initials: I
authors.lastname: Ferchichi
authors.forename: Wafa
authors.initials: W
authors.lastname: Troudi
authors.forename: Raja
authors.initials: R
authors.lastname: Marrakchi
authors.forename: Naziha
authors.initials: N
authors.lastname: Ben Hmida
authors.forename: Sondes
authors.initials: S
authors.lastname: Jebini
authors.forename: Karima
authors.initials: K
authors.lastname: Mrad
authors.forename: Khaled
authors.initials: K
authors.lastname: Ben Romdhane
authors.forename: Amel
authors.initials: A
authors.lastname: Benammar Elgaaied
mesh_terms: D000071443:12E7 Antigen; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000276:immunology; D005260:Female; D006681:HLA-D Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology*; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000175:diagnosis
chemical_list: D000071443:12E7 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D006681:HLA-D Antigens