title: iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis.
abstract: Angiogenic cell therapy represents a novel strategy for ischemic diseases, but some patients show poor responses. We investigated the therapeutic potential of an induced pluripotent stem (iPS) cell sheet created by a novel magnetite tissue engineering technology (Mag-TE) for reparative angiogenesis. Mouse iPS cell-derived Flk-1(+) cells were incubated with magnetic nanoparticle-containing liposomes (MCLs). MCL-labeled Flk-1(+) cells were mixed with diluted extracellular matrix (ECM) precursor and a magnet was placed on the reverse side. Magnetized Flk-1(+) cells formed multi-layered cell sheets according to magnetic force. Implantation of the Flk-1(+) cell sheet accelerated revascularization of ischemic hindlimbs relative to the contralateral limbs in nude mice as measured by laser Doppler blood flow and capillary density analyses. The Flk-1(+) cell sheet also increased the expressions of VEGF and bFGF in ischemic tissue. iPS cell-derived Flk-1(+) cell sheets created by this novel Mag-TE method represent a promising new modality for therapeutic angiogenesis.
authors.affiliation: Department of Cardiology, Nagoya University Graduate School of Medicine.
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Kito
authors.forename: Rei
authors.initials: R
authors.lastname: Shibata
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ishii
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Suzuki
authors.forename: Tatsuhito
authors.initials: T
authors.lastname: Himeno
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kataoka
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yamamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Naomi
authors.initials: N
authors.lastname: Nishio
authors.forename: Sachiko
authors.initials: S
authors.lastname: Ito
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Numaguchi
authors.forename: Tohru
authors.initials: T
authors.lastname: Tanigawa
authors.forename: Jun K
authors.initials: JK
authors.lastname: Yamashita
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Ouchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Honda
authors.forename: Kenichi
authors.initials: K
authors.lastname: Isobe
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Murohara
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006614:Hindlimb / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D007511:Ischemia / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D017078:Laser-Doppler Flowmetry; D008081:Liposomes / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D023822:Tissue Engineering; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D008081:Liposomes; D058185:Magnetite Nanoparticles; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2