title: Interactions between the P6 and P5-1 proteins of southern rice black-streaked dwarf fijivirus in yeast and plant cells.
abstract: Southern rice black-streaked dwarf virus (SRBSDV) is a recently described member of the genus Fijivirus, family Reoviridae. The roles of the proteins encoded by the SRBSDV genome have rarely been studied. In a yeast two-hybrid (YTH) assay in which SRBSDV P6, a putatively multifunctional protein, was used as bait and an SRBSDV cDNA library was used as prey, there was a strong interaction between the P6 and P5-1 proteins. The interaction was confirmed by bimolecular fluorescence complement (BiFC) assay in plant cells. YTH analysis using truncated mutants showed that the N-terminal region (amino acids 9-231) of P5-1 is necessary for binding P5-1 to P6 and that the N-terminal fragment (amino acids 1-93) of P6 is necessary for its interaction with P5-1. SRBSDV P5-1 formed granules positioned at the cell periphery in Nicotiana benthamiana leaves; P6 was present in both the cytoplasm and the nucleus and formed punctate bodies associated with the cell periphery. Immunogold labeling showed that both P6 and P5-1 localized within viroplasms in infected cells of rice plants. These results suggest that the interaction between P5-1 and P6 of SRBSDV may be involved in the formation of viroplasms.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Xue
authors.forename: Heng-Mu
authors.initials: HM
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Lv
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.forename: Yuan
authors.initials: Y
authors.lastname: Meng
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D004252:DNA Mutational Analysis; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D059828:Plant Cells; D025941:Protein Interaction Mapping; D012087:Reoviridae / Q000502:physiology*; D012441:Saccharomyces cerevisiae; D014026:Nicotiana; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins