title: Laboratory diagnosis of malaria in the North West Region of Cameroon: analysis of limitations.
abstract: BACKGROUND
  Malaria is still rife and perennial in Cameroon despite remarkable progress in controlling the disease. About 95% of the country is malaria endemic. Prompt and accurate diagnosis of malaria may lead to improved patient care and reduced morbidity. This paper analyses limitations in malaria diagnosis in the North West Region of Cameroon and opportunities for improvement.
  
  
  METHODS
  The sample units were 40 laboratories in governmental health facilities (GHFs) selected by cluster (Health Districts) and stratified sampling. The three categories of GHFs in the Region - Hospitals, District Medical Centres (Centre Médical d'Arrondissement [CMAs]) and Integrated Health Centres (IHCs) - were strata in the sample. With pre-tested interviewer-administered structured questionnaires and visits to laboratories, mechanical and optical components of microscopes and malaria diagnostic techniques were studied systematically and in detail.
  
  
  RESULTS
  The main finding was that locally prepared Giemsa-stained malaria smears were of unusable quality in 52 and 46% of GHFs for thick and thin smears respectively. Some loss of quality was observed in laboratories with good and moderate quality smears.
  
  
  CONCLUSIONS
  The quality of malaria diagnosis was not optimal and GHFs did not have sufficient tools and resources to overcome loss of quality. Nonetheless, limitations in malaria diagnosis in the Region can be corrected.
authors.affiliation: Faculty of Medicine and Pharmacy, Human Ecology Department, Vrije Universiteit Brussel, Brussels, Belgium. ericndofor@yahoo.fr
authors.forename: Eric
authors.initials: E
authors.lastname: Ndofor
authors.forename: Tom
authors.initials: T
authors.lastname: van Gool
authors.forename: Henk
authors.initials: H
authors.lastname: Gillis
mesh_terms: D002163:Cameroon; D019411:Clinical Laboratory Techniques / Q000592:standards*; D003955:Diagnostic Tests, Routine / Q000592:standards*; D006801:Humans; D008288:Malaria / Q000175:diagnosis*; D008853:Microscopy / Q000592:standards; D013194:Staining and Labeling / Q000592:standards
keywords: Cameroon; Governmental health facilities; Improvement; Malaria; Microscopic diagnosis; Quality