title: Association of decreased mitochondrial DNA content with the progression of colorectal cancer.
abstract: BACKGROUND
  Experimental data suggest that mitochondria is involved in tumorigenesis. However, little is known about the qualitative and quantitative changes of mtDNA in colorectal cancer tissues. We therefore conducted possible correlations of the mitochondrial DNA (mtDNA) copy number in colorectal cancer (CRC) with clinical and pathological findings and CRC prognosis.
  
  
  METHODS
  mtDNA copy numbers in CRC cancer tissue and adjacent non-cancerous tissue samples were measured using quantitative real-time polymerase chain reaction analyses from 60 patients admitted to our hospital. We examined the correlation of mtDNA copy numbers and clinicopathologic parameters of CRC patients. The correlation between mtDNA copy number and three-year survival was analyzed.
  
  
  RESULTS
  The mtDNA copy number was lower in CRC tissue compared with the corresponding non-cancerous colorectal tissue (mean: 108.60 ± 20.11 vs. 153.68 ± 25.72) and was significantly correlated with lymph-node metastasis. Patients with a lower mtDNA copy number tended to have lower 3-year survival than patients with a higher mtDNA copy number assessed by Kaplan-Meier curves, but the correlation was not significant (overall survival, 63.0 vs 83%).
  
  
  CONCLUSIONS
  These results suggest that a reduced copy number of mtDNA is correlated with malignant potential in CRC.
authors.affiliation: Department of Gastroenterology, 305 Hospital of PLA, Beijing, China.
authors.forename: HaiHong
authors.initials: H
authors.lastname: Cui
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: ZhiJing
authors.initials: Z
authors.lastname: Wang
authors.forename: YunXin
authors.initials: Y
authors.lastname: Zhang
authors.forename: ZhenHua
authors.initials: Z
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: XiaoPeng
authors.initials: X
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: XiaoMing
authors.initials: X
authors.lastname: Guo
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D056915:DNA Copy Number Variations; D004272:DNA, Mitochondrial / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D004272:DNA, Mitochondrial