title: The effect of biocontrol bacteria on rhizosphere bacterial communities analyzed by plating and PCR-DGGE.
abstract: Bacillus subtilis B579, which was isolated from rhizosphere of cucumber, exhibited an excellent biocontrol activity on soil-born pathogens under greenhouse conditions. It could colonize in rhizosphere of cucumber with large number of populations after inoculated in plant growth season. To reveal the effect of high level colonization of B. subtilis B579 on rhizobacteria community structure, cultivation-based analysis coupled with denaturing gradient gel electrophoresis (DGGE) analysis were used to profile the changes of rhizobacteria community structure sampling at 1 week interval. Cultivation-based and DGGE fingerprinting analysis showed significant plant-dependent and seasonal shifts in rhizobacteria populations. Only minimal and transient effects were observed at 4-9 weeks after sowing in samples of B579 treatment, without the pathogen inoculation and showed the best plant growth potential. Sequencing of dominant bands excised from the gel revealed that Streptomyces sp. was the dominate species in soils before and after sowing. Burkholderia sp. was the dominate species in bulk soil, while Bacillus sp. was dominated in rhizosphere within the growth season. Arthrobacter ramosus and Nocardioides sp. were identified as the specific species in samples treated by B579 at the maturity and flowering stages of cucumber.
authors.affiliation: School of Pharmaceutical, Liaocheng University, Liaocheng, 252059, Shandong, China. chenfang20045@163.com
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Shuju
authors.initials: S
authors.lastname: Li
authors.forename: Huizhe
authors.initials: H
authors.lastname: Wang
authors.forename: Deduo
authors.initials: D
authors.lastname: Han
authors.forename: Shangjing
authors.initials: S
authors.lastname: Guo
mesh_terms: D000898:Antibiosis; D001412:Bacillus subtilis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D018553:Cucumis sativus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D017753:Ecosystem; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S