title: Degradome sequencing reveals an endogenous microRNA target in C. elegans.
abstract: Caenorhabditis elegans microRNAs (miRNAs) bind to partially complementary sequences in the 3' untranslated region of target mRNAs, resulting in translational repression through mRNA destabilization. High-throughput sequencing of RNA cleavage fragments was performed to directly detect miRNA-directed cleavage targets in adult stage C. elegans. From this analysis, we found that miR-249 directed the cleavage of the ZK637.6 transcript with extensive and evolutionarily conserved complementarity in nematode. In addition, expression of the ZK637.6 transcript was strongly dependent on the expression of miR-249. These findings may lead to a better understanding of miRNA-mediated gene regulation in nematodes.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: June Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Soungyub
authors.initials: S
authors.lastname: Ahn
authors.forename: Soyoung
authors.initials: S
authors.lastname: Kim
authors.forename: Junho
authors.initials: J
authors.lastname: Lee
authors.forename: Jin-Wu
authors.initials: JW
authors.lastname: Nam
authors.forename: Chanseok
authors.initials: C
authors.lastname: Shin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D053501:Arsenite Transporting ATPases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020413:3' Untranslated Regions; D029742:Caenorhabditis elegans Proteins; C581219:MIRN249 microRNA, C elegans; D035683:MicroRNAs; D018510:RNA, Helminth; C518267:ASNA-1 protein, C elegans; D053501:Arsenite Transporting ATPases