title: New procedure for extraction of algal lipids from wet biomass: a green clean and scalable process.
abstract: A new procedure, called Simultaneous Distillation and Extraction Process (SDEP), for lipid extraction from wet microalgae (Nannochloropsis oculata and Dunaliella salina) was reported. This method does not require a pre-drying of the biomass and employs alternative solvents such as d-limonene, α-pinene and p-cymene. This procedure has been compared with Soxhlet extraction (Sox) and Bligh & Dyer method (B&D). For N. oculata, results showed that SDEP-cymene provided similar lipid yields to B&D (21.45% and 23.78%), while SDEP-limonene and pinene provided lower yields (18.73% and 18.75% respectively). For D. salina, SDEP-pinene provided the maximum lipid yield (3.29%) compared to the other solvents, which is quite close to B&D result (4.03%). No significant differences in terms of distribution of lipid classes and fatty acid composition have been obtained for different techniques. Evaluation of energy consumption indicates a substantial saving in the extraction cost by SDEP compared to the conventional extraction technique, Soxhlet.
authors.affiliation: University of Avignon, INRA, UMR408, 84000 Avignon, France.
authors.forename: Celine
authors.initials: C
authors.lastname: Dejoye Tanzi
authors.forename: Maryline
authors.initials: M
authors.lastname: Abert Vian
authors.forename: Farid
authors.initials: F
authors.lastname: Chemat
mesh_terms: D018533:Biomass; D002855:Chromatography, Thin Layer; D057168:Distillation / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis; D055772:Green Chemistry Technology / Q000379:methods*; D008055:Lipids / Q000145:classification / Q000302:isolation & purification*; D058086:Microalgae / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D005227:Fatty Acids; D008055:Lipids