title: Structural elucidation of an extracellular polysaccharide produced by the marine fungus Aspergillus versicolor.
abstract: A homogenous extracellular polysaccharide, designated AWP, was isolated from the fermented liquid of the marine fungus Aspergillus versicolor from the coral Cladiella sp. and purified by anion-exchange and size-exclusion chromatography (SEC). Chemical and spectroscopic analyses, including one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) spectroscopy showed that AWP consisted of glucose and mannose in a molar ratio of 8.6:1.0, and its average molecular weight was estimated to be 500kDa. AWP is a slightly branched extracellular polysaccharide. The backbone of AWP is mainly composed of (1→6)-linked α-D-glucopyranose residues, slightly branched by single α-d-mannopyranose units attached to the main chain at C-3 positions of the glucan backbone. The investigation demonstrated that AWP is a novel extracellular polysaccharide different from those of other marine microorganisms.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mao
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiancun
authors.initials: X
authors.lastname: Teng
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu
authors.forename: Yanli
authors.initials: Y
authors.lastname: Chen
authors.forename: Chunqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.forename: Mengxia
authors.initials: M
authors.lastname: Yan
authors.forename: Jimiao
authors.initials: J
authors.lastname: Shan
authors.forename: Cong
authors.initials: C
authors.lastname: Lin
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology; D001230:Aspergillus / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis / Q000737:chemistry; D008745:Methylation; D015394:Molecular Structure; D008970:Molecular Weight; D025282:Mycelium / Q000737:chemistry*
chemical_list: D062610:Fungal Polysaccharides; D005947:Glucose; D008358:Mannose