title: Pertussis toxoid structure: a collaboration and comparison of two-dimensional liquid chromatography-tandem mass spectrometry, ultraperformance liquid chromatography-mass spectrometryE, and capillary liquid chromatography-matrix-assisted laser desorption ionization-tandem mass spectrometry.
abstract: The overall structure of pertussis toxoid has been established by analysis of its tryptic digest using two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS), capillary liquid chromatography-matrix-assisted laser desorption ionization-tandem mass spectrometry (CapLC-MALDI-MS/MS), and ultraperformance liquid chromatography-mass spectrometry(E) (UPLC-MS(E)). In addition to oxidation and hydrolysis of amino acids losses of terminal peptides are observed. On-line UPLC-MS(E) generated a similar sequence coverage as the other two methods that involved off-line fraction collection. In light of recent favorable comparisons to data-dependent acquisition, UPLC-MS(E) should be the initial method of choice for analysis of a peptide mixture of moderate complexity.
authors.affiliation: Baxter Healthcare Inc., Round Lake, IL 60073, USA.
authors.forename: Manorama
authors.initials: M
authors.lastname: Tummala
authors.forename: Almary
authors.initials: A
authors.lastname: Chacon
authors.forename: Edward K
authors.initials: EK
authors.lastname: Chess
authors.forename: Shwu-Maan
authors.initials: SM
authors.lastname: Lee
authors.forename: Peifeng
authors.initials: P
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014121:Toxoids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D014121:Toxoids; C051093:pertussis toxoid