title: Inhibitory activity of green tea (Camellia sinensis) extract on some clinically isolated cariogenic and periodontopathic bacteria.
abstract: OBJECTIVE
  To determine the in vitro inhibitory activity of green tea extract on some clinically isolated cariogenic and periodontopathic bacteria.
  
  
  METHODS
  Twenty strains of each of Streptococcusmutans, Aggregatibacteractinomycetemcomitans, Porphyromonasgingivalis, and Prevotellaintermedia were isolated from carious teeth and periodontal pockets of patients with dental caries and periodontal diseases. Green tea extract was prepared by aqueous extraction method and diluted from 50 to 1.56 mg/ml. Standard techniques of agar disk diffusion and broth microdilution assays were applied for qualitative and quantitative determinations of antibacterial activity of green tea extract on each isolates.
  
  
  RESULTS
  All clinical isolates of S. mutans (100%) were sensitive to green tea extract at concentrations 6.25, 12.5, 25, and 50 mg/ml producing inhibition zones ranging from 10 to 38 mm. All periodontopathic isolates (A. actinomycetemcomitans, n = 20, P. intermedia, n = 20, and P. gingivalis, n = 20) (100%) tested were sensitive to 12.5, 25, and 50 mg/ml of this extract. The minimal inhibitory concentration of green tea extract for S. mutans was 3.28 ± 0.7 mg/ml and for A. actinomycetemcomitans 6.25, for P. gingivalis and P. intermedia 12.5 mg/ml.
  
  
  CONCLUSIONS
  Our findings showed that green tea extract exhibited strong antibacterial activity on S. mutans,A. actinomycetemcomitans,P. gingivalis and P. intermedia and therefore may be used in mouthwashes or dentifrices for prevention of dental caries and periodontal diseases.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. abdolmehdi@gmail.com
authors.forename: Abdolmehdi
authors.initials: A
authors.lastname: Araghizadeh
authors.forename: Jamshid
authors.initials: J
authors.lastname: Kohanteb
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Fani
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects*; D028241:Camellia sinensis; D003731:Dental Caries / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D010510:Periodontal Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D018713:Prevotella intermedia / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: D010936:Plant Extracts