title: Identification and characterization of antifungal active substances of Streptomyces hygroscopicus BS-112.
abstract: An antifungal Actinomyces BS-112 strain, with Aspergillus flavus as the target pathogen, was isolated from soil in the forest land of Mountain Tai. This strain showed a strong antagonistic activity against various mold fungi in food and feed. Strain BS-112 was identified as Streptomyces hygroscopicus based on its morphologic, cultural, physiological, biochemical characteristics, cell wall components and 16S rDNA sequence. Four active components were separated and purified from strain BS-112. These four antifungal components were identified as tetrins A and B and tetramycins A and B using spectroscopic analysis including mass spectrometry and nuclear magnetic resonance spectroscopy. Tetrins A and B and tetramycins A and B strongly inhibited the growth of A. flavus, A. alutaceus, A. niger, and A. fumigatus in vitro.
authors.affiliation: College of Life Sciences, Neijiang Normal University, Neijiang 641112, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Song
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Xie
authors.forename: Ping
authors.initials: P
authors.lastname: Cui
authors.forename: Hongxia
authors.initials: H
authors.lastname: Jiang
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Ruicheng
authors.initials: R
authors.lastname: Ju
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jinyu
authors.initials: J
authors.lastname: Li
authors.forename: Xunli
authors.initials: X
authors.lastname: Liu
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000378:metabolism*; D001230:Aspergillus / Q000187:drug effects / Q000254:growth & development; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D018942:Macrolides; C003421:tetrin B; C003420:tetrin A