title: EROD activity induction in peripheral blood lymphocytes, liver and brain tissues of rats orally exposed to polycyclic aromatic hydrocarbons.
abstract: Little is known in terms of multi-matrix cytochrome P450 activity induction under repeated oral exposure to planar halogenated and polycyclic aromatic hydrocarbons (PHH, PAH). In the present study, 60 rats were daily exposed, during 28 days, to oral ingestion of a mixture consisting of phenanthrene, pyrene and benzo(a)pyrene at 0, 6 or 600 μg/day. EROD activity, reflecting almost exclusively CYP1A1 and CYP1B1 activities, was measured in brain and liver microsomes as well as in peripheral blood lymphocytes (PBLs). All induction kinetics could be appropriately fitted using logistic-like models. After 28 days of exposure to a 6 μg/day dose, EROD activity was found to be 91, 152 and 94-fold increased in lymphocytes, liver and brain, respectively, compared to day 0. Plateau activities could be appropriately fitted versus ingested doses using Hill or Michaelis-Menten models. Correlations between matrices made it possible to conclude that EROD activity in PBL should be considered as a sensitive, convenient and non-destructive approach for (i) evaluating EROD activity in liver, which was found to represent 98% of the observed EROD activities in the three tested matrices and (ii) evaluating oral exposure of homogeneous groups of farm animals (race, diet) to CYP inducing PAH and PHH.
authors.affiliation: Research Unit on Animal and Functionality of Animal Products (UR AFPA), University of Lorraine, ENSAIA, 2 avenue de la Forêt de Haye, BP 172, 54505 Vandoeuvre-lès-Nancy, France.
authors.forename: Abir
authors.initials: A
authors.lastname: Chahin
authors.forename: Julie
authors.initials: J
authors.lastname: Peiffer
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Olry
authors.forename: Guillemette
authors.initials: G
authors.lastname: Crepeaux
authors.forename: Henri
authors.initials: H
authors.lastname: Schroeder
authors.forename: Guido
authors.initials: G
authors.lastname: Rychen
authors.forename: Yann
authors.initials: Y
authors.lastname: Guiavarc'h
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism / Q000633:toxicity*; D011721:Pyrenes / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010616:Phenanthrenes; D011721:Pyrenes; D001564:Benzo(a)pyrene; C031181:phenanthrene; C030984:pyrene; D019363:Cytochrome P-450 CYP1A1