title: A peptide nucleic acid (PNA) heteroduplex probe containing an inosine-cytosine base pair discriminates a single-nucleotide difference in RNA.
abstract: Selective discrimination of a single-nucleotide difference in single-stranded DNA or RNA remains a challenge with conventional DNA or RNA probes. A peptide nucleic acid (PNA)-derived probe, in which PNA forms a pseudocomplementary heteroduplex with inosine-containing DNA or RNA, effectively discriminates a single-nucleotide difference in a closely related group of sequences of single-stranded DNA and/or RNA. The pseudocomplementary PNA heteroduplex is easily converted to a fluorescent probe that distinctively detects a member of highly homologous let-7 microRNAs.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Eiji
authors.initials: E
authors.lastname: Nakata
authors.forename: Tomoki
authors.initials: T
authors.lastname: Tamura
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Aizawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000032:analysis; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007288:Inosine / Q000032:analysis; D035683:MicroRNAs / Q000032:analysis; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D015341:Nucleic Acid Probes / Q000138:chemical synthesis* / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D035683:MicroRNAs; D009692:Nucleic Acid Heteroduplexes; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; D007288:Inosine; D012313:RNA; D003596:Cytosine; D004247:DNA