title: A comparative study of nitrilases identified by genome mining.
abstract: Escherichia coli strains expressing different nitrilases transformed nitriles or KCN. Six nitrilases (from Aspergillus niger (2), A. oryzae, Neurospora crassa, Arthroderma benhamiae, and Nectria haematococca) were arylacetonitrilases, two enzymes (from A. niger and Penicillium chrysogenum) were cyanide hydratases and the others (from P. chrysogenum, P. marneffei, Gibberella moniliformis, Meyerozyma guilliermondi, Rhodococcus rhodochrous, and R. ruber) preferred (hetero)aromatic nitriles as substrates. Promising nitrilases for the transformation of industrially important substrates were found: the nitrilase from R. ruber for 3-cyanopyridine, 4-cyanopyridine and bromoxynil, the nitrilases from N. crassa and A. niger for (R,S)-mandelonitrile, and the cyanide hydratase from A. niger for KCN and 2-cyanopyridine.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Kaplan
authors.forename: Alicja B
authors.initials: AB
authors.lastname: Veselá
authors.forename: Alena
authors.initials: A
authors.lastname: Petříčková
authors.forename: Fabrizia
authors.initials: F
authors.lastname: Pasquarelli
authors.forename: Martina
authors.initials: M
authors.lastname: Pičmanová
authors.forename: Anna
authors.initials: A
authors.lastname: Rinágelová
authors.forename: Tek Chand
authors.initials: TC
authors.lastname: Bhalla
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pátek
authors.forename: Ludmila
authors.initials: L
authors.lastname: Martínková
mesh_terms: D000619:Aminohydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005658:Fungi / Q000201:enzymology / Q000235:genetics; D016681:Genome, Fungal; D023281:Genomics; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D000619:Aminohydrolases; C021867:nitrilase; D006836:Hydro-Lyases; C076848:cyanide hydratase