title: Kolaviron prevents carbendazim-induced steroidogenic dysfunction and apoptosis in testes of rats.
abstract: The study evaluated the protective role of kolaviron (an isolated biflavonoid from the seed of Garcinia kola) and vitamin E in carbendazim-induced reproductive dysfunction in male rats. Adult male Wistar rats were orally exposed to carbendazim (200mg/kg) singly or in combination with kolaviron (100 and 200mg/kg). Exposure to carbendazim significantly decreased the activities of superoxide dismutase and catalase but markedly increased sialic acid concentration and lipid peroxidation in the testes of rats. Western blot analysis revealed that carbendazim treatment decreased the expression of steroid acute regulatory (StAR) protein and androgen binding protein (ABP) with concomitant decrease in activities of steroidogenic enzymes. Germ cell apoptosis in carbendazim-treated rats was confirmed by TUNEL assay. However, pretreatment with kolaviron and vitamin E restored the testicular antioxidant status and steroidogenesis and decreased apoptotic nuclei to near control level in carbendazim-treated rats. Kolaviron may prove useful in combating carbendazim-induced reproductive toxicity.
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.
authors.forename: I A
authors.initials: IA
authors.lastname: Adedara
authors.forename: S
authors.initials: S
authors.lastname: Vaithinathan
authors.forename: R
authors.initials: R
authors.lastname: Jubendradass
authors.forename: P P
authors.initials: PP
authors.lastname: Mathur
authors.forename: E O
authors.initials: EO
authors.lastname: Farombi
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000727:Androgen-Binding Protein / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001562:Benzimidazoles / Q000633:toxicity*; D002219:Carbamates / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000633:toxicity*; D029763:Garcinia kola; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D000727:Androgen-Binding Protein; D001562:Benzimidazoles; D002219:Carbamates; C491899:Fas protein, mouse; D005419:Flavonoids; D005659:Fungicides, Industrial; D010750:Phosphoproteins; D010936:Plant Extracts; D019014:fas Receptor; D000098682:Steroidogenic Acute Regulatory Protein; D045304:Cytochromes c; D015096:3-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases; D002374:Catalase; D013482:Superoxide Dismutase; D053148:Caspase 3; D019158:N-Acetylneuraminic Acid; C006698:carbendazim; C045492:kolaviron