title: A cytotoxic hydroperoxy sterol from the brown alga, Nizamuddinia zanardinii.
abstract: BACKGROUND
  The marine environment is a unique source of bioactive natural products, of which Nizamuddinia zanardinii is an important brown algae distributed in Oman Sea. Literature revealed that there is no report on phytochemistry and pharmacology of this valuable algae.
  
  
  METHODS
  Bioguided fractionation of the methanolic extract of Nizamuddinia zanardinii, collected from Oman Sea, led to the isolation of a hydroperoxy sterol. Its structure was determined by analysis of the spectroscopic data as 24-hydroperoxy-24-vinyl cholesterol (HVC). In vitro cytotoxic activity of this compound was evaluated against HT29, MCF7, A549, HepG2 and MDBK cell lines.
  
  
  RESULTS
  Although 24(R)-hydroproxy-24-vinylcholesterol has been previously reported from Sargassum and Padina species, it is the first report on the presence of this compound from N. zanardinii. This compound exhibited cytotoxicity in all cell lines (IC50, 3.62, 9.09, 17.96, 32.31 and 37.31 μg/mL respectively). HVC was also evaluated for apoptotic activity and demonstrated positive results in terminal deoxynucleotidyl transferase dUTP Nick End labeling (TUNEL) assay suggesting it a candidate for further apoptotic studies.
  
  
  CONCLUSIONS
  Nizamuddinia zanardinii, a remarkable brown algae of Oman Sea, is a good source of hydroproxy sterols with promising cytotoxic on various cell lines particularly human colon adenocarcinoma.
authors.affiliation: Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. goharii_a@tums.ac.ir.
authors.forename: Maryam Hamzeloo
authors.initials: MH
authors.lastname: Moghadam
authors.forename: Jamileh
authors.initials: J
authors.lastname: Firouzi
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Saeidnia
authors.forename: Homa
authors.initials: H
authors.lastname: Hajimehdipoor
authors.forename: Shahla
authors.initials: S
authors.lastname: Jamili
authors.forename: Abdolhossein
authors.initials: A
authors.lastname: Rustaiyan
authors.forename: Ahmad R
authors.initials: AR
authors.lastname: Gohari