title: A 3D porous polymer monolith-based platform integrated in poly(dimethylsiloxane) microchips for immunoassay.
abstract: In this work, we demonstrate the immunocapture and on-line fluorescence immunoassay of protein and virus based on porous polymer monoliths (PPM) in microfluidic devices. Poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) [poly(GMA-co-EGDMA)] monoliths were successfully synthesized in the polydimethylsiloxane (PDMS) microfluidic channels by in situ UV-initiated free radical polymerization. After surface modification, PPM provides a high-surface area and specific affinity 3D substrate for immunoassays. Combining with well controlled microfluidic devices, the direct immunoassay of IgG and sandwich immunoassay of inactivated H1N1 influenza virus using 5 μL sample has been accomplished, with detection limits of 4 ng mL(-1) and less than 10 pg mL(-1), respectively. The enhanced detection sensitivity is due to both high surface area of PPM and flow-through design. The detection time was obviously decreased mainly due to the shortened diffusion distance and improved convective mass transfer inside the monolith, which accelerates the reaction kinetics between antigen and antibody. This work provides a novel microfluidic immunoassay platform with high efficiency thereby enabling fast and sensitive immunoassay.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Qin-Shu
authors.initials: QS
authors.lastname: Kang
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Shen
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Hu
authors.forename: Meng-Jia
authors.initials: MJ
authors.lastname: Hu
authors.forename: Hui
authors.initials: H
authors.lastname: Liao
authors.forename: Han-Zhong
authors.initials: HZ
authors.lastname: Wang
authors.forename: Zhi-Ke
authors.initials: ZK
authors.lastname: He
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D005026:Ethylene Glycols; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000032:analysis*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D058105:Polymerization; D016062:Porosity; D012680:Sensitivity and Specificity
chemical_list: D004129:Dimethylpolysiloxanes; D005026:Ethylene Glycols; D007074:Immunoglobulin G; D008689:Methacrylates; C526534:poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate); C013830:baysilon