title: QuaNCAT: quantitating proteome dynamics in primary cells.
abstract: Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors.forename: Andrew J M
authors.initials: AJ
authors.lastname: Howden
authors.forename: Vincent
authors.initials: V
authors.lastname: Geoghegan
authors.forename: Kristin
authors.initials: K
authors.lastname: Katsch
authors.forename: Georgios
authors.initials: G
authors.lastname: Efstathiou
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Bhushan
authors.forename: Omar
authors.initials: O
authors.lastname: Boutureira
authors.forename: Benjamin
authors.initials: B
authors.lastname: Thomas
authors.forename: David C
authors.initials: DC
authors.lastname: Trudgian
authors.forename: Benedikt M
authors.initials: BM
authors.lastname: Kessler
authors.forename: Daniela C
authors.initials: DC
authors.lastname: Dieterich
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Davis
authors.forename: Oreste
authors.initials: O
authors.lastname: Acuto
mesh_terms: D000596:Amino Acids; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D061207:Calcium Ionophores / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002853:Chromatography, Liquid / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007553:Isotope Labeling; D010703:Phorbol Esters / Q000494:pharmacology; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000596:Amino Acids; D061207:Calcium Ionophores; D002273:Carcinogens; D010703:Phorbol Esters; D015759:Ionomycin