title: Cumulus and granulosa cell markers of oocyte and embryo quality.
abstract: Lack of an objective, accurate, and noninvasive embryo assessment strategy remains one of the major challenges encountered in in vitro fertilization. Cumulus and mural granulosa cells reflect the characteristics of the oocyte, providing a noninvasive means to assess oocyte quality. Specifically, transcriptomic profiling of follicular cells may help identify biomarkers of oocyte and embryo competence. Current transcriptomics technologies include quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for analysis of individual genes and microarrays and high-throughput deep sequencing for whole genome expression profiling. Recently, using qRT-PCR and microarray technologies, a multitude of studies correlated changes in cumulus or granulosa cell gene expression with clinically relevant outcome parameters, including in vitro embryo development and pregnancy. While the initial findings are promising, a clinical benefit from the use of identified biomarker genes remains to be demonstrated in randomized controlled trials.
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520-8063, USA.
authors.forename: Asli
authors.initials: A
authors.lastname: Uyar
authors.forename: Saioa
authors.initials: S
authors.lastname: Torrealday
authors.forename: Emre
authors.initials: E
authors.lastname: Seli
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D054885:Cumulus Cells / Q000166:cytology / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods* / Q000639:trends; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006107:Granulosa Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy
chemical_list: D015415:Biomarkers; D005819:Genetic Markers