title: Selective detection of NADPH among four pyridine-nucleotide cofactors by a fluorescent probe based on aggregation-induced emission.
abstract: A fluorescent sensor based on guanidinium-tethered tetraphenylethene (TPE) has been investigated toward the differentiation of pyridine nucleotide cofactors (NAD(+) , NADH, NADP(+) , and NADPH). TPE selectively recognizes NADPH possessing the higher tetra-anionic net-charge, resulting in the steep "turn-on" fluorescence increase. The comparative aggregation behaviors and fluorescence response studies of TPE on the four cofactors reveal that the critical aggregate concentration of TPE against NADPH correlates directly with the concentration threshold for the fluorescence response. These results establish that TPE can selectively differentiate NADPH over the other three cofactors by the steep aggregation-induced fluorescence response accompanied by the high signal-to-background contrast.
authors.affiliation: Institute for Advanced Study, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Noguchi
authors.forename: Arnab
authors.initials: A
authors.lastname: Dawn
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yoshihara
authors.forename: Youichi
authors.initials: Y
authors.lastname: Tsuchiya
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Seiji
authors.initials: S
authors.lastname: Shinkai
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009249:NADP / Q000032:analysis* / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides; D011725:Pyridines; D009249:NADP; C023666:pyridine