title: Achyrocline satureioides (Lam.) D.C. Hydroalcoholic Extract Inhibits Neutrophil Functions Related to Innate Host Defense.
abstract: Achyrocline satureioides (Lam.) D.C. is a herb native to South America, and its inflorescences are popularly employed to treat inflammatory diseases. Here, the effects of the in vivo actions of the hydroalcoholic extract obtained from inflorescences of A. satureioides on neutrophil trafficking into inflamed tissue were investigated. Male Wistar rats were orally treated with A. satureioides extract, and inflammation was induced one hour later by lipopolysaccharide injection into the subcutaneous tissue. The number of leukocytes and the amount of chemotactic mediators were quantified in the inflammatory exudate, and adhesion molecule and toll-like receptor 4 (TLR-4) expressions and phorbol-myristate-acetate- (PMA-) stimulated oxidative burst were quantified in circulating neutrophils. Leukocyte-endothelial interactions were quantified in the mesentery tissue. Enzymes and tissue morphology of the liver and kidney were evaluated. Treatment with A. satureioides extract reduced neutrophil influx and secretion of leukotriene B4 and CINC-1 in the exudates, the number of rolling and adhered leukocytes in the mesentery postcapillary venules, neutrophil L-selectin, β 2-integrin and TLR-4 expression, and oxidative burst, but did not cause an alteration in the morphology and activities of liver and kidney. Together, the data show that A. satureioides extract inhibits neutrophil functions related to the innate response and does not cause systemic toxicity.
authors.affiliation: Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 Bl 13B, 05508-900 São Paulo, SP, Brazil.
authors.forename: Eric Diego
authors.initials: ED
authors.lastname: Barioni
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Santin
authors.forename: Isabel Daufenback
authors.initials: ID
authors.lastname: Machado
authors.forename: Stephen Fernandes de Paula
authors.initials: SF
authors.lastname: Rodrigues
authors.forename: Viviane
authors.initials: V
authors.lastname: Ferraz-de-Paula
authors.forename: Theodoro Marcel
authors.initials: TM
authors.lastname: Wagner
authors.forename: Bruno
authors.initials: B
authors.lastname: Cogliati
authors.forename: Matheus
authors.initials: M
authors.lastname: Corrêa Dos Santos
authors.forename: Marina da Silva
authors.initials: Mda S
authors.lastname: Machado
authors.forename: Sérgio Faloni
authors.initials: SF
authors.lastname: de Andrade
authors.forename: Rivaldo
authors.initials: R
authors.lastname: Niero
authors.forename: Sandra Helena Poliselli
authors.initials: SH
authors.lastname: Farsky