title: Nedd8 processing enzymes in Schizosaccharomyces pombe.
abstract: BACKGROUND
  Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1.
  
  
  RESULTS
  Here we show that in the fission yeast, Schizosaccharomyces pombe, the Yuh1 orthologue, Uch1, is not the sole Nedd8 processing enzyme. Instead it appears that deubiquitylating enzymes can efficiently process the Nedd8 precursor in vivo.
  
  
  CONCLUSIONS
  Several enzymes contribute to Nedd8 precursor processing including a number of deubiquitylating enzymes.
authors.affiliation: MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
authors.forename: Jean E
authors.initials: JE
authors.lastname: O'Donoghue
authors.forename: Dawadschargal
authors.initials: D
authors.lastname: Bech-Otschir
authors.forename: Ida B
authors.initials: IB
authors.lastname: Larsen
authors.forename: Mairi
authors.initials: M
authors.lastname: Wallace
authors.forename: Rasmus
authors.initials: R
authors.lastname: Hartmann-Petersen
authors.forename: Colin
authors.initials: C
authors.lastname: Gordon
mesh_terms: D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012568:Schizosaccharomyces / Q000201:enzymology / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D044842:Cullin Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases