title: Comparison of methanogenic community structure and anaerobic process performance treating swine wastewater between pilot and optimized lab scale bioreactors.
abstract: To investigate methanogenic community structure and process performance of anaerobic digestion treating swine wastewater at different scale, a pilot plant with 20 m(3) of effective working volume and lab scale methanogenic digester with 6L working volume were operated for 71 days and 6 turnover periods, respectively. During the steady state of anaerobic digestion, COD and VS removal efficiency in pilot plant were 65.3±3.2, 51.6±4.3%, respectively, which was similar to those in lab scale. However, calculated VFAs removal efficiency and methane yield were lower in pilot plant than in lab scale digester. Also, organics removal efficiencies, which consist of total carbohydrates, proteins, and lipids, were different between pilot and lab scale. These results were thought to be due to the ratio of carbohydrates to proteins in the raw swine wastewater. As a result of qualitative microbial analysis, Methanoculleus receptaculii, and Methanoculleus bourgensis, were commonly concerned with methane production.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, Republic of Korea.
authors.forename: Woong
authors.initials: W
authors.lastname: Kim
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Cho
authors.forename: Seungyong
authors.initials: S
authors.lastname: Lee
authors.forename: Seokhwan
authors.initials: S
authors.lastname: Hwang
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001483:Base Sequence; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004926:Escherichia coli; D005232:Fatty Acids, Volatile / Q000378:metabolism; D008372:Manure / Q000382:microbiology*; D008697:Methane / Q000096:biosynthesis*; D019638:Methanomicrobiaceae / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013552:Swine; D014865:Waste Disposal, Fluid / Q000295:instrumentation* / Q000379:methods*
chemical_list: D017931:DNA Primers; D005232:Fatty Acids, Volatile; D008372:Manure; D008697:Methane
keywords: Anaerobic digestion; COD; CSTR; DDW; DGGE; HRT; Lab scale; MY; NCBI; NLRI; National Center for Biotechnology Information; National Livestock Research Institute; OLR; Optimized; Pilot scale; Swine wastewater; TC; TS; TVFAs; VFAs; VS; VSS; chemical oxygen demand; continuously stirred tank reactor; deionized and distilled water; denaturating gradient gel electrophoresis; hydraulic retention time; methane yield; organic loading rate; total carbohydrates; total solids; total volatile fatty acids; volatile fatty acids; volatile solids; volatile suspended solids