title: Transcriptional profiling of Dictyostelium with RNA sequencing.
abstract: Transcriptional profiling methods have been utilized in the analysis of various biological processes in Dictyostelium. Recent advances in high-throughput sequencing have increased the resolution and the dynamic range of transcriptional profiling. Here we describe the utility of RNA sequencing with the Illumina technology for production of transcriptional profiles. We also describe methods for data mapping and storage as well as common and specialized tools for data analysis, both online and offline.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Edward Roshan
authors.initials: ER
authors.lastname: Miranda
authors.forename: Gregor
authors.initials: G
authors.lastname: Rot
authors.forename: Marko
authors.initials: M
authors.lastname: Toplak
authors.forename: Balaji
authors.initials: B
authors.lastname: Santhanam
authors.forename: Tomaz
authors.initials: T
authors.lastname: Curk
authors.forename: Gad
authors.initials: G
authors.lastname: Shaulsky
authors.forename: Blaz
authors.initials: B
authors.lastname: Zupan
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D057225:Data Mining; D004023:Dictyostelium / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D018503:Genome, Protozoan; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan