title: Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture.
abstract: Traditional skin grafting techniques are effective but limited methods of skin replacement. Autologous transplantation of rapidly cultured keratinocytes is successful for epidermal regeneration, but the current gold-standard technique requires mouse fibroblast feeders and serum-rich media, with serum-free systems and dermal fibroblast (DF) feeders performing relatively poorly. Here, we investigated the capacity of human hair follicle dermal cells to act as alternative supports for keratinocyte growth. Dermal papilla (DP) dermal sheath (DS), DF and 3T3 cells were used as inactivated feeder cells for human keratinocyte coculture. Under conditions favouring dermal cells, proliferation of keratinocytes in the presence of either DS or DP cells was significantly enhanced compared with DF cells, at levels comparable to keratinocytes cultured under gold-standard conditions. Secreted protein acidic and rich in cysteine (SPARC) expression increased DS and DP cells relative to DFs; however, further experiments did not demonstrate a role in keratinocyte support.
authors.forename: Rebecca P
authors.initials: RP
authors.lastname: Hill
authors.forename: Aaron
authors.initials: A
authors.lastname: Gardner
authors.forename: Heather C
authors.initials: HC
authors.lastname: Crawford
authors.forename: Rachel
authors.initials: R
authors.lastname: Richer
authors.forename: Anna
authors.initials: A
authors.lastname: Dodds
authors.forename: William A
authors.initials: WA
authors.lastname: Owens
authors.forename: Clifford
authors.initials: C
authors.lastname: Lawrence
authors.forename: Sam
authors.initials: S
authors.lastname: Rao
authors.forename: Bo
authors.initials: B
authors.lastname: Kara
authors.forename: S Elizabeth
authors.initials: SE
authors.lastname: James
authors.forename: Colin A
authors.initials: CA
authors.lastname: Jahoda
mesh_terms: D016475:3T3 Cells / Q000166:cytology; D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D049109:Cell Proliferation; D018920:Coculture Techniques; D020405:Dermis / Q000166:cytology* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D005353:Fibronectins / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D051379:Mice; D015676:Osteonectin; D016038:Skin Transplantation / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D005353:Fibronectins; D007797:Laminin; D015676:Osteonectin; C577241:SPARC protein, human; D025521:Tumor Suppressor Proteins