title: Pathogenesis of tropical sprue: a pilot study of antroduodenal manometry, duodenocaecal transit time & fat-induced ileal brake.
abstract: OBJECTIVE
  Small intestinal bacterial overgrowth (SIBO) due to ileal brake-induced hypomotility may cause tropical sprue (TS). We evaluated effect of infusion of fat or placebo in duodenum randomly in patients with TS and healthy controls on antroduodenal manometry (ADM) and mediators of ileal brake, and duodenocaecal transit time (DCTT).
  
  
  METHODS
  ADM and DCTT (lactulose hydrogen breath test, HBT) were evaluated with placebo and fat in eight controls and 13 patients with TS (diagnostic criteria: tests showing malabsorption of two unrelated substances, abnormal duodenal histology, absence of other causes, response to antibiotics and folate).
  
  
  RESULTS
  Patients with TS (6 had SIBO by glucose HBT) were similar in age and gender with controls. After fat infusion, proximal gut motility index (MI) was reduced compared to fasting state in TS, and DCTT was longer in TS than controls (200 min, 120-380 vs. 130, 70-160, P=0.001), though comparable after placebo (70 min, 30-140 vs. 60, 40-90). TS patients had higher PYY and neurotensin than controls after fat infusion. DCTT after fat infusion correlated with plasma level of PYY in TS but not in controls. Post-fat PYY and neurotensin levels were higher in TS with lower BMI (<16 kg/m [2] ) than those with higher BMI. Parameters of ileal brake (post-fat DCTT, PYY and neurotensin) were higher in patients with than without SIBO.
  
  
  CONCLUSIONS
  Fat infusion reduced proximal gut MI, increased DCTT, PYY, and neurotensin among patients with TS. Malabsorbed fat might cause exaggerated ileal brake reducing gut motility, promoting SIBO and bacterial colonization and malabsorption in TS.
authors.affiliation: Department of Gastroenterology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India. udayghoshal@gmail.com
authors.forename: Uday C
authors.initials: UC
authors.lastname: Ghoshal
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Asha
authors.initials: A
authors.lastname: Misra
authors.forename: Gourdas
authors.initials: G
authors.lastname: Choudhuri
mesh_terms: D000328:Adult; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D004382:Duodenitis / Q000382:microbiology / Q000503:physiopathology; D005215:Fasting; D005260:Female; D005772:Gastrointestinal Transit; D006801:Humans; D007082:Ileum / Q000382:microbiology / Q000503:physiopathology*; D007421:Intestine, Small / Q000382:microbiology / Q000503:physiopathology*; D008297:Male; D008365:Manometry; D008875:Middle Aged; D009496:Neurotensin / Q000008:administration & dosage; D019894:Peptide YY / Q000008:administration & dosage; D013182:Sprue, Tropical / Q000188:drug therapy* / Q000382:microbiology / Q000503:physiopathology*
chemical_list: D019894:Peptide YY; D009496:Neurotensin