title: Cloning and characterization of the fatty acid-binding protein gene from the protoscolex of Taenia multiceps.
abstract: Taenia multiceps (Cestoda: Taeniidae), a worldwide cestode parasite, is emerging as an important helminthic zoonosis due to serious or fatal central nervous system disease commonly known as coenurosis in domestic and wild ruminants including humans. Herein, a fatty acid-binding protein (FABP) gene was identified from transcriptomic data in T. multiceps. This gene, which contains a complete coding sequence, was amplified by reverse-transcriptase polymerase chain reaction. The corresponding protein, which was named TmFABP, had a molecular weight of 14 kDa, and subsequently was recombinantly expressed in Escherichia coli. The fusion protein was purified on Ni-NTA beads (Bio-Rad). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analyses showed that the purified recombinant protein caused immunogenicity. Immunohistochemical studies showed that TmFABP was expressed at the tegumental level in the protoscolices and in the cells between the body wall and parenchyma layer of the cestode. In sections from gravid proglottids, intense staining was detected in the uterus and eggs. Based on this, TmFABP could be switched on during differentiation of germinative layers to protoscoleces and from metacestodes to adult worms. Taken together, our results already reported for T. multiceps suggest the possibility of TmFABP developing a vaccine to control and prevent coenurosis.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, 625014, China.
authors.forename: Hua-Ming
authors.initials: HM
authors.lastname: Nie
authors.forename: Yue
authors.initials: Y
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.forename: Ying-Dong
authors.initials: YD
authors.lastname: Yang
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Gu
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Lai
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Peng
authors.forename: Guang-You
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015511:Goat Diseases / Q000469:parasitology; D006041:Goats / Q000469:parasitology*; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013621:Taenia / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D013622:Taeniasis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D015801:Helminth Proteins; D011994:Recombinant Proteins