title: Functional imaging of Rel expression in inflammatory processes using bioluminescence imaging system in transgenic mice.
abstract: c-Rel plays important roles in many inflammatory diseases. Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs. In this paper, a transgenic mouse line, B6-Tg(c-Rel-luc)(Mlit), which incorporated the transgene firefly luciferase driven by a 14.5-kb fragment containing mouse c-Rel gene Rel promoter, was generated to monitor Rel expression in vivo. Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model. The luciferase expression in transgenic mice was comparable to the endogenous Rel expression and could be suppressed by administration of anti-inflammatory drug dexamethasone or aspirin. These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Jing
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Ruilin
authors.initials: R
authors.lastname: Sun
authors.forename: Zhenze
authors.initials: Z
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Ying
authors.forename: Lei
authors.initials: L
authors.lastname: Ci
authors.forename: Ying
authors.initials: Y
authors.lastname: Kuang
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Zhugang
authors.initials: Z
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Fei
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D049409:Luciferases, Firefly / Q000235:genetics; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D057054:Molecular Imaging; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000009:adverse effects; D010446:Peptide Fragments / Q000009:adverse effects; D020569:Proto-Oncogene Proteins c-rel / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D020569:Proto-Oncogene Proteins c-rel; D012333:RNA, Messenger; C445081:myelin oligodendrocyte glycoprotein (35-55); D003907:Dexamethasone; D015054:Zymosan; D049409:Luciferases, Firefly; D001241:Aspirin