title: Cardioprotective and antioxidant activities of a polysaccharide from the root bark of Aralia elata (Miq.) Seem.
abstract: A water-soluble polysaccharide (AEP-w1) was isolated from the root bark of Aralia elata and its molecular weight was about 4.5×10(4)Da. Monosaccharide component analysis indicated that AEP-w1 appeared to be arabinogalactan, consisting of arabinose, galactose and trace glucose with molar ratios of 6.3:3.5:0.2. The antioxidant and cardioprotective potential of AEP-w1 were evaluated in vitro for the first time. AEP-w1 showed potent superoxide and hydroxyl radical scavenging activities and reducing power in vitro. Treatment with H2O2 resulted in the death of H9c2 cells, whereas pretreatment with 50-400μg/ml AEP-w1 for 24h prior to H2O2 exposure significantly increased cell viability. Furthermore, AEP-w1 evidently suppressed cardiomyocyte apoptosis, the mitochondrial membrane potential change and cytochrome c release in H2O2-treated H9c2 cells. In addition, intracellular reduced glutathione (GSH) reduction caused by H2O2 in H9c2 cells was also restored by AEP-w1 pretreatment. Taken together, these date provided the first evidence that the cardioprotective effects of AEP-w1 in H9c2 cells were at least in part associated with its antioxidant activity and inhibition effect on mitochondrial dysfunction.
authors.affiliation: Department of Cardiology, Tangdu Hospital, The Fourth Military Medical University, Xian 710038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Xue
authors.forename: Qiangsun
authors.initials: Q
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D027983:Aralia / Q000737:chemistry*; D002316:Cardiotonic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016923:Cell Death; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005978:Glutathione / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects; D018384:Oxidative Stress; D024301:Plant Bark / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012995:Solubility; D013997:Time Factors
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D011134:Polysaccharides; D006861:Hydrogen Peroxide; D005978:Glutathione