title: [Establishment of a high-throughput respiratory virus detection technology without RNA purification and reverse transcription].
abstract: OBJECTIVE
  To establish a convenient and high-throughput respiratory virus detection method to facilitate epidemiological viral monitoring.
  
  
  METHODS
  We used high-throughput microsphere-based flexible multi-analyte profiling technology (xMAP) coupled with signal amplification molecules to simultaneously detect RNAs of 8 viruses including influenza viruses A and B, parainfluenza viruses type 1, 2 and 3, respiratory syncytial viruses A and B, and metapneumovirus in a 96-well plate format. The sensitivity and specificity of the method for the synthetic viral RNAs were evaluated.
  
  
  RESULTS
  There was no cross-reactivity among the 8 respiratory viral target RNAs. The detection limits for the 8 viral in intro-transcribed RNAs ranged from 1204 to 4695 RNA copies.
  
  
  CONCLUSIONS
  We establish a sensitive, specific, convenient, and high-throughput multiplex detection method suitable for detecting multiple respiratory viral RNAs for epidemiological viral monitoring.
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Dan-li
authors.initials: DL
authors.lastname: Yang
authors.forename: Xiao-yi
authors.initials: XY
authors.lastname: Tian
authors.forename: Wei-xian
authors.initials: WX
authors.lastname: Shi
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods*; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification; D009981:Influenza B virus / Q000235:genetics / Q000302:isolation & purification; D057230:Limit of Detection; D029121:Metapneumovirus / Q000235:genetics / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification; D012137:Respiratory System / Q000821:virology*; D018108:Respirovirus / Q000235:genetics / Q000302:isolation & purification; D048348:Reverse Transcription; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral