title: Sperm penetration to the zona pellucida of an oocyte: a computational model incorporating acrosome reaction.
abstract: For fertilisation to occur, a spermatozoon needs to cross the zona pellucida (ZP), which is a glycoprotein layer surrounding the oocyte. Crossing the ZP requires an acrosome reaction (AR) where enzymes released from the spermatozoon head locally digest and soften the ZP so that the spermatozoon can penetrate deeper. Here, a biomechanical sperm-oocyte interaction model that considers the AR using the finite element method was formulated. This modelling is used to determine which of the following factors directly contribute to the crossing of the ZP: local ZP softening by AR, sperm head shape, ZP hardening elsewhere than in the AR site, ZP thickness and sperm hyperactivation (more flagellar beating). It has been found that an AR softening the ZP to over one-tenth of its basal stiffness is important for successful sperm penetration, and that 'sharper' heads have a biomechanical advantage in penetrating deeper. The approach is promising for understanding this exciting stage of reproduction.
authors.affiliation: a Department of Biomedical Engineering , Faculty of Engineering, Tel Aviv University , Tel Aviv , 69978 , Israel.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kozlovsky
authors.forename: Amit
authors.initials: A
authors.lastname: Gefen
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology*; D003198:Computer Simulation; D020342:Finite Element Analysis; D006801:Humans; D008297:Male; D008954:Models, Biological; D009865:Oocytes / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology; D015044:Zona Pellucida / Q000502:physiology