title: Establishing an in vivo p48ZnF bioluminescence mouse brain imaging model.
abstract: p48ZnF is a C3H1 zinc finger domain-containing protein that is involved in the control of gene transcription and translation. In the present study a novel transgenic p48ZnF mouse model is described that is useful for in vivo brain imaging using luciferase as bioluminescence-mediating reporter gene. Yeast two-hybrid screening and western blot analyses revealed Drg1 (developmentally regulated GTP binding protein 1) and Pcbp1 (poly (rC)-binding protein 1) as p48ZnF-associated proteins. Interestingly, p48ZnF' cellular location of action depends on the cell's differentiation status: nuclear in proliferating cells and cytoplasmic in differentiated neurons.
authors.affiliation: Department of Biomedical Engineering, College of Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea. klaus@hanyang.ac.kr
authors.forename: Klaus
authors.initials: K
authors.lastname: Heese
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018703:Founder Effect; D019204:GTP-Binding Proteins / Q000378:metabolism; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D016716:PC12 Cells; D016601:RNA-Binding Proteins; D051381:Rats; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C121375:Pcbp1 protein, mouse; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C495885:Zc3h15 protein, rat; C078418:developmentally regulated GTP-binding protein; D008156:Luciferases; D019204:GTP-Binding Proteins