title: Comparison of the effects of predilution and postdilution hemodiafiltration on neutrophils, lymphocytes and platelets.
abstract: Numerous studies have been carried out to investigate the solute removal efficiency of hemodiafiltration (HDF). However, the effect of the dilution mode on blood cell damage during HDF has not yet been examined in detail. Here, we compared predilution and postdilution HDF with respect to their effects on blood cells. Five patients were allocated to one session each of predilution HDF and postdilution HDF. Concentrations of interleukin (IL)-6, intercellular adhesion molecule (ICAM)-1, and platelet-derived microparticles (PDMP), and the phagocytotic and sterilizing functions of neutrophils before and after the HDF sessions were evaluated. Lymphocyte blastoid transformation induced by mitogens was also evaluated by measurement of the [(3)H]-thymidine uptake. The IL-6 and ICAM-1 concentrations decreased after predilution HDF, and increased after postdilution HDF. Lymphocyte blastoid transformation was more pronounced after predilution HDF than after postdilution HDF. There was no significant difference in PDMP between the dilution modes. We conclude that predilution HDF could be more favorable for dialysis patients than postdilution HDF from the point of view of the effects on the blood cells, especially neutrophils and lymphocytes.
authors.affiliation: Hashimoto Clinic, 3-21-5 Hashimoto, Midori-ku, Sagamihara-shi, Kanagawa, 252-0143, Japan, ssakurai@peach.ocn.ne.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakurai
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Fumi
authors.initials: F
authors.lastname: Yamauchi
authors.forename: Daiki
authors.initials: D
authors.lastname: Asahi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hosoya
mesh_terms: D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000166:cytology*; D005260:Female; D017583:Hemodiafiltration / Q000379:methods*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy*; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology*
chemical_list: D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1