title: [Establishment of a human laryngeal carcinoma Hep-2/5-Fu cell line and the screening of differentially expressed genes].
abstract: OBJECTIVE
  To establish a Hep-2/5-Fu of human drug-resistant laryngeal carcinoma cell line, and to screen the possible drug resistance-associated genes.
  
  
  METHODS
  Hep-2/5-Fu of a human drug resistant laryngeal carcinoma cell line was induced by continuously exposing human laryngeal carcinoma cells to gradually increasing concentrations of 5-Fu. The growth law was observed and the growth curve was protracted. The drug resistance of Hep-2/5-Fu was measured by MTT assay and the drug resistant index RI was calculated. Genes expressed differentially between Hep-2/5-Fu and its parent cell line Hep-2 were screened using a gene chip, and several selected drug resistance associated genes were confirmed by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Compared with its parental cells, the drug resistance cell line had slower growth rate and larger size. The Hep-2/5-Fu cell line showed cross drug resistance to 5-Fu, cisplatin and vincristine. There were 1210 differentially expressed genes possibly associated with drug resistance by the gene chip screening method. The possible drug resistance-related genes included Cyclin D, IGF-BP3, CASP9, and CDK4/6. The expression of Cyclin D in the Hep-2/5-Fu cell line was 6.5997 times of that in the parent cell line. RT-PCR results were consistent with the gene chip results.
  
  
  CONCLUSIONS
  The altered biological properties of Hep-2/5-Fu may be related to its drug resistance phenotype. Several genes, such as Cyclin D, are possibly involved in the mechanism of drug resistance in this cell line.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renji Hospital of Shanghai JiaoTong University School of Medicine, Institution of Otorhinolaryngology, Shanghai 200001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Jiadong
authors.initials: J
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000235:genetics*; D005472:Fluorouracil / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005472:Fluorouracil