title: Passive dosing of polycyclic aromatic hydrocarbon (PAH) mixtures to terrestrial springtails: linking mixture toxicity to chemical activities, equilibrium lipid concentrations, and toxic units.
abstract: A 7-day mixture toxicity experiment with the terrestrial springtail Folsomia candida was conducted, and the effects were linked to three different mixture exposure parameters. Passive dosing from silicone was applied to tightly control exposure levels and compositions of 12 mixture treatments, containing the polycyclic aromatic hydrocarbons (PAHs) naphthalene, phenanthrene, and pyrene. Springtail lethality was then linked to sum chemical activities (∑a), sum equilibrium lipid concentrations (∑C(lipid eq.)), and sum toxic units (∑TU). In each case, the effects of all 12 mixture treatments could be fitted to one sigmoidal exposure-response relationship. The effective lethal chemical activity (La50) of 0.027 was well within the expected range for baseline toxicity of 0.01-0.1. Linking the effects to the lipid-based exposure parameter yielded an effective lethal concentration (LC(lipid eq 50)) of 133 mmol kg(-1) lipid in good correspondence with the lethal membrane burden for baseline toxicity (40-160 mmol kg(-1) lipid). Finally, the effective lethal toxic unit (LTU50) of 1.20 was rather close to the expected value of 1. Altogether, passive dosing provided tightly controlled mixture exposure in terms of both level and composition, while ∑a, ∑C(lipid eq.), and ∑TU allowed baseline toxicity to be linked to mixture exposure.
authors.affiliation: Department of Environmental Science, Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark. stns@dmu.dk
authors.forename: Stine N
authors.initials: SN
authors.lastname: Schmidt
authors.forename: Martin
authors.initials: M
authors.lastname: Holmstrup
authors.forename: Kilian E C
authors.initials: KE
authors.lastname: Smith
authors.forename: Philipp
authors.initials: P
authors.lastname: Mayer
mesh_terms: D000818:Animals; D001181:Arthropods / Q000187:drug effects*; D004347:Drug Interactions; D008055:Lipids / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*
chemical_list: D008055:Lipids; D011084:Polycyclic Aromatic Hydrocarbons