title: Carbon-Ag/AgCl probes for detection of cell activity in droplets.
abstract: In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan. ino.kosuke@bioinfo.che.tohoku.ac.jp
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Kaoru
authors.initials: K
authors.lastname: Ono
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Arai
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D000627:Aminophenols / Q000032:analysis / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000592:standards; D004566:Electrodes; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D012834:Silver / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry*
chemical_list: D000627:Aminophenols; D018030:Silver Compounds; D012834:Silver; D002244:Carbon; D000469:Alkaline Phosphatase; C037548:silver chloride; C026729:4-aminophenol