title: Cell proliferation, viability, and in vitro differentiation of equine mesenchymal stem cells seeded on bacterial cellulose hydrogel scaffolds.
abstract: The culture of multipotent mesenchymal stem cells on natural biopolymers holds great promise for treatments of connective tissue disorders such as osteoarthritis. The safety and performance of such therapies relies on the systematic in vitro evaluation of the developed stem cell-biomaterial constructs prior to in vivo implantation. This study evaluates bacterial cellulose (BC), a biocompatible natural polymer, as a scaffold for equine-derived bone marrow mesenchymal stem cells (EqMSCs) for application in bone and cartilage tissue engineering. An equine model was chosen due to similarities in size, load and types of joint injuries suffered by horses and humans. Lyophilized and critical point dried BC hydrogel scaffolds were characterized using scanning electron microscopy (SEM) to confirm nanostructure morphology which demonstrated that critical point drying induces fibre bundling unlike lyophilisation. EqMSCs positively expressed the undifferentiated pluripotent mesenchymal stem cell surface markers CD44 and CD90. The BC scaffolds were shown to be cytocompatible, supporting cellular adhesion and proliferation, and allowed for osteogenic and chondrogenic differentiation of EqMSCs. The cells seeded on the BC hydrogel were shown to be viable and metabolically active. These findings demonstrate that the combination of a BC hydrogel and EqMSCs are promising constructs for musculoskeletal tissue engineering applications.
authors.affiliation: Department of Materials Science and Engineering, College of Engineering, University of Tennessee, Knoxville, TN 37996, USA.
authors.forename: Pelagie M
authors.initials: PM
authors.lastname: Favi
authors.forename: Roberto S
authors.initials: RS
authors.lastname: Benson
authors.forename: Nancy R
authors.initials: NR
authors.lastname: Neilsen
authors.forename: Ryan L
authors.initials: RL
authors.lastname: Hammonds
authors.forename: Cassandra C
authors.initials: CC
authors.lastname: Bates
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Stephens
authors.forename: Madhu S
authors.initials: MS
authors.lastname: Dhar
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000494:pharmacology* / Q000648:ultrastructure; D020219:Chondrogenesis / Q000187:drug effects; D005612:Freeze Drying; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects; D013194:Staining and Labeling; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002482:Cellulose; D000469:Alkaline Phosphatase