title: An injectable cell penetrable nano-polyplex hydrogel for localized siRNA delivery.
abstract: An approach for application of cell penetration to selective small interference RNA (siRNA) localized delivery system, cell penetrable nano-polyplex assembled hydrogel system, is presented. The cell penetrable nano-polyplex assembled hydrogelisprepared by protamine conjugation to poly(organophos-phazene) and inducement of nano-polyplexes with siRNAs. After an injection of cell penetrable nano-polyplex solution into the body, it turns into a gel due to thermosensitivity of poly(organo- phosphazene). The gel maintains up to 4 weeks and the released 30 nm-sized nano-polyplexes from the gel induces highly effective siRNA delivery due to cell penetration. Accordingly, the new system shows a high gene silencing efficiency on only the target site in long-term with a single injection.
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Park
authors.forename: Soo-Chang
authors.initials: SC
authors.lastname: Song
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D024202:Electrophoretic Mobility Shift Assay; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007267:Injections; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011479:Protamines / Q000737:chemistry; D034741:RNA, Small Interfering / Q000378:metabolism*; D044967:Serum; D055672:Static Electricity; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D009943:Organophosphorus Compounds; D011108:Polymers; D011479:Protamines; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D016650:Fluorescein-5-isothiocyanate