title: [Effect of homoharringtonine on expression of NF-κB and BCL-2 proteins in K562 cells].
abstract: This study was aimed to investigate the effect of homoharringtonine (HHT) on K562 cell proliferation, apoptosis and expression of BCL-2 and NF-κB proteins. The cells proliferation was assayed with MTT method, the cell apoptosis, cell cycle and BCL-2 expression were analyzed with flow cytometry, NF-κB protein expression was detected with Western blot. The results showed that HHT concentration-dependently inhibited proliferation of K562 cells, the IC50 at 48 h was 43.89 ng/ml. Treated with HHT 10 ng/ml for 48 h, K562 cell apoptosis significantly increased, cell cycle was blocked at G0/G1, the expression level of BCL-2 and NF-κB proteins was lower than that in control group (P < 0.05). It is concluded that HHT may inhibit the proliferation of K562 cells, and down-regulating expression levels of BCL-2 and NF-κB may be one of its anti-CML mechanisms.
authors.affiliation: Department of Hematology, Nanjing Medical University, Huaian, Jiangsu Province, China.
authors.forename: Li-Lin
authors.initials: LL
authors.lastname: Ye
authors.forename: Wei-Ke
authors.initials: WK
authors.lastname: Cao
authors.forename: Yu-Ye
authors.initials: YY
authors.lastname: Shi
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Deng
authors.forename: Shan-Dong
authors.initials: SD
authors.lastname: Tao
authors.forename: Ping
authors.initials: P
authors.lastname: Ji
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Fu
authors.forename: Ming-Zhu
authors.initials: MZ
authors.lastname: Zong
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D005434:Flow Cytometry; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D020014:K562 Cells; D016328:NF-kappa B / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
chemical_list: D006248:Harringtonines; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D000077863:Homoharringtonine