title: Migration and activation of T cells during development of graft-versus-host disease in a mouse model.
abstract: OBJECTIVE
  We investigated the pathophysiologic events in graft-versus-host disease (GVHD), a major complication of hematopoietic stem cell transplantation (HSCT).
  
  
  METHODS
  The experimental group included BALB/c female mice conditioned with 8.0 GY total body irradiation that were transplanted with allogeneic C57BL/6 male bone marrow cells (BMCs) plus CD4(+) T cells and CD8(+) T cells isolated from green fluorescent protein transgenic (eGFP-Tg) C57BL/6 male mice by the immunomagnetic beads negative sorting method. The control group was transplanted only with C57BL/6 male BMCs.
  
  
  RESULTS
  GVHD clinical manifestations were present in the BMCs plus T-cell-transplanted group, but not the BMCs alone group. eGFP(+) T cells were observed in recipient organs, including the liver, spleen, intestine, skin, lungs, tongue, kidneys and even the brain. Donor eGFP(+) T cells were significantly increased in liver and spleen before day +4 (P < .05); but decreased in the spleen while still increased in the liver after day +4 (P < .05). CD25 expression of donor eGFP(+) T cells in the liver and spleen, and interleukin (IL)-2 levels in the peripheral blood was significantly increased before day +4 (P < .05), but decreased after day +4 (P < .05).
  
  
  CONCLUSIONS
  These data support the donor T-cell migration hypothesis that accompanied by expression of CD25 and IL-2, during the development of GVHD donor T cells migrate to lymphoid organs, such as the spleen, after activation migrating to GVHD target organs to induce GVHD damage.
authors.affiliation: Oncology and Hematology Department, Ningbo Development Zone Center Hospital, Ningbo, PR China. WHSJL@163.com
authors.forename: H-S
authors.initials: HS
authors.lastname: Wen
authors.forename: J-M
authors.initials: JM
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: S-I
authors.initials: SI
authors.lastname: Gong
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D002634:Chemotaxis, Leukocyte; D004195:Disease Models, Animal; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D018189:Immunomagnetic Separation; D007376:Interleukin-2 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013997:Time Factors; D014916:Whole-Body Irradiation
chemical_list: C508018:Il2ra protein, mouse; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D049452:Green Fluorescent Proteins