title: Comparative evaluation of biocompatibility of dense nanostructured and microstructured Hydroxyapatite/Titania composites.
abstract: This work deals with the biocompatibility of dense nano- and micro-structured Hydroxyapatite/Titania composites prepared by two step and conventional sintering, respectively. By application of two step sintering, it was shown that the final grain size of HA-15 wt.% TiO2 is maintained lower than 100 nm while by the application of conventional sintering it reaches higher than 100 nm. Biocompatibility of the dense bulks was evaluated by cell attachment and proliferation experiments. Cell morphology, and viability on each nano- and micro-structured Hydroxyapatite/Titania composites were examined at different time points. The nanostructured HA/Titania dense bulk exhibited higher cell viability than a microstructured one. In addition, the effects of ionic products from nano- and micro-structured bulk dissolution on osteoblasts were studied. The MTT test confirmed that the products from nanostructured HA/Titania dense bulk significantly promoted osteoblast proliferation within a certain concentration range.
authors.affiliation: Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 8415683111, Iran. a.farzin@ma.iut.ac.ir
authors.forename: A
authors.initials: A
authors.lastname: Farzin
authors.forename: M
authors.initials: M
authors.lastname: Ahmadian
authors.forename: M H
authors.initials: MH
authors.lastname: Fathi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D008422:Materials Testing / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011208:Powders; D051381:Rats; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011208:Powders; C009495:titanium dioxide; D017886:Durapatite; D014025:Titanium