title: Surface-mediated nucleic acid delivery by lipoplexes prepared in microwell arrays.
abstract: Many delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acid-based therapeutics. A facile surface-mediated nucleic acid delivery by lipoplexes is prepared in a microwell array, which combines the advantages of lipoplexes as an efficient carrier system, surface-mediated delivery, and the control of surface topography. Uniform disc-like lipoplexes containing nucleic acids are formed in the microwell array with a diameter of ∼818 nm and thickness of ∼195 nm. The microwell array-mediated delivery of lipoplexes containing FAM-oligodeoxynucleotides is ∼18.6 and ∼10.6 times more efficient than the conventional transfection method in an adherent cell line (A549 non-small cell lung cancer cells) and a suspension cell line (KG-1a acute myelogenous leukemia cells), respectively. MicroRNA-29b is then used as a model nucleic acid to investigate the therapeutic efficacy of lipoplexes delivered by the microwell array. Compared to conventional transfection methods, the effective therapeutic dosage of microRNA-29b is reduced from the microgram level to the nanogram level by lipoplexes prepared in the microwell array. The microwell array is also a very flexible platform. Both nucleic acid therapeutics and imaging reagents are incorporated in lipoplexes and successfully delivered to A549 cells, demonstrating its potential applications in theranostic medicine.
authors.affiliation: Nanoscale Science and Engineering Center for Affordable, Nanoengineering, The Ohio State University, 174 W 18th Avenue, Room 1012, Columbus, Ohio 43210, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.forename: Megan Cavanaugh
authors.initials: MC
authors.lastname: Terp
authors.forename: Kwang Joo
authors.initials: KJ
authors.lastname: Kwak
authors.forename: Daniel
authors.initials: D
authors.lastname: Gallego-Perez
authors.forename: Serge P
authors.initials: SP
authors.lastname: Nana-Sinkam
authors.forename: L James
authors.initials: LJ
authors.lastname: Lee
mesh_terms: D002232:Carbocyanines / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics; D005434:Flow Cytometry; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009696:Nucleic Acids / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D045663:Quantum Dots; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties
chemical_list: D002232:Carbocyanines; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D008055:Lipids; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D009696:Nucleic Acids; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C085321:cyanine dye 5
keywords: lipoplexes; microwell arrays; nuleic acid delivery; theranostic medicine