title: Differences in the phenolic composition and antioxidant properties between Vitis coignetiae and Vitis vinifera seeds extracts.
abstract: Phenolic compounds were extracted from European and Japanese grapevine species (Vitis vinifera and V. coignetiae) seeds using 80% methanol or 80% acetone. The total content of phenolic compounds was determined utilizing Folin-Ciocalteu's phenol reagent, while the content of tannins was assayed by the vanillin and BSA precipitation methods. Additionally, the DPPH free radical and ABTS cation radical scavenging activities and the reduction power of the extracts were measured. The HPLC method was applied to determine the phenolic compounds, such as phenolic acids and catechins. The seeds contained large amounts of tannins and gallic acid and observable quantities of catechins, p-coumaric, ferulic and caffeic acids. The dominant form of phenolic acids in the extracts was the ester-bound form. The content of total phenolics was higher in the European grape V. vinifera seeds, which also contained more tannins, catechins and phenolic acids, except for caffeic acid. Extracts from V. vinifera seeds showed better radical scavenger properties and stronger reducing power. The total contents of phenolic compounds and tannins in acetone extracts were higher than in methanolic extracts. Acetone extracts also exhibited stronger antiradical properties as well as stronger reducing power.
authors.affiliation: Department of Biochemistry, University of Warmia and Mazury, Oczapowskiego Street 1A, Olsztyn 10-957, Poland. weidner@uwm.edu.pl
authors.forename: Stanisław
authors.initials: S
authors.lastname: Weidner
authors.forename: Anna
authors.initials: A
authors.lastname: Rybarczyk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Karamać
authors.forename: Angelika
authors.initials: A
authors.lastname: Król
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Mostek
authors.forename: Joanna
authors.initials: J
authors.lastname: Grębosz
authors.forename: Ryszard
authors.initials: R
authors.lastname: Amarowicz
mesh_terms: D000096:Acetone / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D005609:Free Radicals / Q000737:chemistry; D056604:Grape Seed Extract / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000302:isolation & purification; D000432:Methanol / Q000737:chemistry; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D019163:Reducing Agents / Q000737:chemistry / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification; D027843:Vitis / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D056604:Grape Seed Extract; D062385:Hydroxybenzoates; D010851:Picrates; D019163:Reducing Agents; D012997:Solvents; D013451:Sulfonic Acids; D013634:Tannins; D000096:Acetone; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002392:Catechin; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol