title: GOLPH3 regulates the migration and invasion of glioma cells though RhoA.
abstract: Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in the development of several human cancers. However, the biological significance of GOLPH3 in glioma progression remains largely unknown. In this study, we report, for the first time, that downregulation of GOLPH3 led to clear reductions in glioma cell migration and invasion. In addition, downregulation of GOLPH3 inhibited the expression of the small GTPase RhoA as well as cytoskeletal reorganization, which are both required for glioma cell migration. Furthermore, we found that the observed reductions in glioma cell migration and RhoA level could be rescued by RhoA overexpression. Taken together, these results show that GOLPH3 contributes to the motility of glioma cells by regulating the expression of RhoA.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China. xpzhou@xzmc.edu.cn
authors.forename: Xiuping
authors.initials: X
authors.lastname: Zhou
authors.forename: Wenjian
authors.initials: W
authors.lastname: Zhan
authors.forename: Wenbin
authors.initials: W
authors.lastname: Bian
authors.forename: Lei
authors.initials: L
authors.lastname: Hua
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
authors.forename: Shao
authors.initials: S
authors.lastname: Xie
authors.forename: Dongxu
authors.initials: D
authors.lastname: Yang
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Guanzheng
authors.initials: G
authors.lastname: Liu
authors.forename: Rutong
authors.initials: R
authors.lastname: Yu
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology; D015194:Diffusion Chambers, Culture; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010957:Plasmids; D015398:Signal Transduction; D014162:Transfection; D020742:rhoA GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C429024:GOLPH3 protein, human; D008565:Membrane Proteins; D020742:rhoA GTP-Binding Protein