title: Molecular mechanism of 9-cis-retinoic acid inhibition of adipogenesis in 3T3-L1 cells.
abstract: Retinoic acid (RA) signaling is mediated by specific nuclear hormone receptors. Here we examined the effects of 9-cis-RA on adipogenesis in mouse preadipocyte 3T3-L1 cells. 9-cis-RA inhibits the lipid accumulation of adipogenetically induced 3T3-L1 cells. The complex of retinoid X receptor α (RXRα) with peroxisome proliferator-activated receptor γ (PPARγ) is a major transcription factor in the process of adipogenesis, and the levels of these molecules were decreased by 9-cis-RA treatment. A RXR pan-antagonist suppressed 9-cis-RA's inhibitory effects on adipogenesis, but not on the intracellular levels of both RXRα and PPARγ. These results suggest that 9-cis-RA could inhibit adipogenesis by activating RXR, and decrease both RXR and PPARγs levels in a RXR activation-independent manner.
authors.affiliation: Laboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Sagara
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kagechika
authors.forename: Noriko
authors.initials: N
authors.lastname: Takahashi
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects* / Q000502:physiology*; D000077556:Alitretinoin; D000818:Animals; D003984:Dibenzazepines / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism; D047490:Retinoid X Receptor alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D003984:Dibenzazepines; C104183:LE 540; D047495:PPAR gamma; D018168:Receptors, Retinoic Acid; D047490:Retinoid X Receptor alpha; D000077556:Alitretinoin; D014212:Tretinoin