title: (1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
abstract: Acinetobacter baumannii is an aerobic and gram-negative pathogenic bacterium that is resistant to most antibiotics. Recently, A. baumannii 1656-2 exhibited the ability to form biofilms under clinical conditions. In this study, global metabolite profiling of both planktonic and biofilm forms of A. baumannii 1656-2 was performed using high-resolution nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis to investigate the metabolic patterns leading to biofilm formation. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed a distinct separation between planktonic and biofilm cells. Metabolites including acetates, pyruvate, succinate, UDP-glucose, AMP, glutamate, and lysine were increasingly involved in the energy metabolism of biofilm formation. In particular, the ratio of N-acetyl-D-glucosamine (GlcNAc) to D-glucosamine (GlcNH2) was significantly higher during biofilm formation than under the planktonic condition. This study demonstrates that NMR-based global metabolite profiling of bacterial cells can provide valuable insight into the metabolic changes in multidrug resistant and biofilm-forming bacteria such as A. baumannii 1656-2.
authors.affiliation: Integrated Metabolomics Research Group, Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea.
authors.forename: Jinki
authors.initials: J
authors.lastname: Yeom
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Shin
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Yang
authors.forename: Jungmin
authors.initials: J
authors.lastname: Kim
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
mesh_terms: D040981:Acinetobacter baumannii / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D010933:Plankton / Q000166:cytology / Q000378:metabolism*; D025341:Principal Component Analysis