title: Expression of P-selectin (CD62P) on platelets after thrombin and ADP in hypotrophic and healthy, full-term newborns.
abstract: BACKGROUND
  Hypotrophic newborns in comparison with eutrophic newborns demonstrate a reduced blood platelet count and therefore may have haemostasis disorders. Expression of P-selectin (CD62P) on the surface of platelets is a marker of stimulated, activated blood platelets. The ability of platelets to react can be determined after the addition of the following activators: strong (thrombin) and weak (ADP).
  
  
  METHODS
  We studied 48 hypotrophic newborns, 25 females and 23 males, weighing less than the 10th centile and 55 healthy, full-term newborns, 25 females and 30 males. Expression of CD62P on the surface of platelets was examined in the native state, after the addition of thrombin or ADP.
  
  
  RESULTS
  Hypotrophic newborns exhibited almost double the percentage of platelets expressing CD62P compared with the control group, 4.21% versus 2.88%. After the addition of thrombin, the percentage was 31.5% versus 12.5%, p < 0.001, whereas after the addition of ADP, the percentage was 9.54% versus 4.5%, p = 0.002.
  
  
  CONCLUSIONS
  Hypotrophic newborns are capable of greater platelet activation in comparison with healthy term newborns. However, gender does not affect the expression of P-selectin.
authors.affiliation: Department of Neonatology, Medical University of Bialystok, 15-276 Bialystok, ul. MC. Sklodowskiej-Curie 24a, Poland. awasiluk@umb.edu.pl
authors.forename: Alicja
authors.initials: A
authors.lastname: Wasiluk
authors.forename: Halina
authors.initials: H
authors.lastname: Kemona
authors.forename: Maria
authors.initials: M
authors.lastname: Mantur
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Polewko
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ozimirski
authors.forename: Robert
authors.initials: R
authors.lastname: Milewski
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005865:Gestational Age; D006262:Health; D006801:Humans; D007230:Infant, Low Birth Weight / Q000378:metabolism; D007231:Infant, Newborn; D008297:Male; D019007:P-Selectin / Q000378:metabolism*; D015539:Platelet Activation / Q000187:drug effects; D047929:Term Birth / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D019007:P-Selectin; D000244:Adenosine Diphosphate; D013917:Thrombin