title: N-terminal palmitoylation is required for Toxoplasma gondii HSP20 inner membrane complex localization.
abstract: Toxoplasma gondii is an obligate intracellular parasite and the causative agent of toxoplasmosis. Protein palmitoylation is known to play roles in signal transduction and in enhancing the hydrophobicity of proteins thus contributing to their membrane association. Global inhibition of protein palmitoylation has been shown to affect T. gondii physiology and invasion of the host cell. However, the proteins affected by this modification have been understudied. This paper shows that the small heat shock protein 20 from T. gondii (TgHSP20) is synthesized as a mature protein in the cytosol and is palmitoylated in three cysteine residues. However, its localization at the inner membrane complex (IMC) is dependent only on N-terminal palmitoylation. Absence or incomplete N-terminal palmitoylation causes TgHSP20 to partially accumulate in a membranous structure. Interestingly, TgHSP20 palmitoylation is not responsible for its interaction with the daughter cells IMCs. Together, our data describe the importance of palmitoylation in protein targeting to the IMC in T. gondii.
authors.affiliation: Universidad Nacional de San Martín, Consejo Nacional de Investigaciones Científicas y Técnicas, Provincia de Buenos Aires, Argentina.
authors.forename: M G
authors.initials: MG
authors.lastname: De Napoli
authors.forename: N
authors.initials: N
authors.lastname: de Miguel
authors.forename: M
authors.initials: M
authors.lastname: Lebrun
authors.forename: S N J
authors.initials: SN
authors.lastname: Moreno
authors.forename: S O
authors.initials: SO
authors.lastname: Angel
authors.forename: M M
authors.initials: MM
authors.lastname: Corvi
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D003545:Cysteine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D050886:HSP20 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D007425:Intracellular Membranes / Q000378:metabolism*; D054878:Lipoylation; D009154:Mutation / Q000235:genetics; D021381:Protein Transport; D015398:Signal Transduction; D014122:Toxoplasma / Q000254:growth & development / Q000378:metabolism*; D014123:Toxoplasmosis / Q000378:metabolism / Q000469:parasitology*
chemical_list: D050886:HSP20 Heat-Shock Proteins; D003545:Cysteine