title: The effects of the synonymous codon usage and tRNA abundance on protein folding of the 3C protease of foot-and-mouth disease virus.
abstract: The 3C protease of foot-and-mouth disease virus (FMDV) has a conserved amino acid sequence and is responsible for most cleavage in the viral polyprotein. The effects of the synonymous codon usage of FMDV 3C gene and tRNA abundance of the hosts on shaping different folding units (α-helix, β-strand and the coil) in the 3C protease were analyzed based on the structural information of the FMDV 3C protease from Protein Data Bank (PDB: 2BHG) and 210 genes of 3C for all serotypes of FMDV. The strong correlation between some codons usage and the specific folding unit in the FMDV 3C protease is found. As for the effect of translation speed caused by tRNA abundance on the formation of folding units, the codon positions with lowly abundant tRNA scatter in the 3C gene and there is the obvious fluctuation of tRNA abundance locating in the transition boundaries from the β-strand to the α-helix and the coil, respectively. However, codon positions with lowly abundant tRNA clustering into these boundaries are not found, suggesting that the adjustment of translation speed is likely also achieved by the single codon position with low tRNA abundance rather than a cluster. The observations can provide the information for insight into the role of the synonymous codon usage in the formation of 3C protease of FMDV and effect of the tRNA abundance of the hosts on this formation of protease.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046 Gansu, PR China.
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Zhou
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: You
authors.forename: Hao-tai
authors.initials: HT
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Li-na
authors.initials: LN
authors.lastname: Ma
authors.forename: Yao-zhong
authors.initials: YZ
authors.lastname: Ding
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Pejsak
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Liu
mesh_terms: D000086042:3C Viral Proteases; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D017124:Conserved Sequence / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D029544:Foot-and-Mouth Disease Virus / Q000201:enzymology / Q000235:genetics*; D017510:Protein Folding; D012343:RNA, Transfer / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003062:Codon; D014764:Viral Proteins; D012343:RNA, Transfer; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases