title: Binding characteristics of homogeneous molecularly imprinted polymers for acyclovir using an (acceptor-donor-donor)-(donor-acceptor-acceptor) hydrogen-bond strategy, and analytical applications for serum samples.
abstract: This paper demonstrates a novel approach to assembling homogeneous molecularly imprinted polymers (MIPs) based on mimicking multiple hydrogen bonds between nucleotide bases by preparing acyclovir (ACV) as a template and using coatings grafted on silica supports. (1)H NMR studies confirmed the AAD-DDA (A for acceptor, D for donor) hydrogen-bond array between template and functional monomer, while the resultant monodisperse molecularly imprinted microspheres (MIMs) were evaluated using a binding experiment, high performance liquid chromatography (HPLC), and solid phase extraction. The Langmuir isothermal model and the Langmuir-Freundlich isothermal model suggest that ACV-MIMs have more homogeneous binding sites than MIPs prepared through normal imprinting. In contrast to previous MIP-HPLC columns, there were no apparent tailings for the ACV peaks, and ACV-MIMs had excellent specific binding properties with a Ka peak of 3.44 × 10(5)M(-1). A complete baseline separation is obtained for ACV and structurally similar compounds. This work also successfully used MIMs as a specific sorbent for capturing ACV from serum samples. The detection limit and mean recovery of ACV was 1.8 ng/mL(-1) and 95.6%, respectively, for molecularly imprinted solid phase extraction coupled with HPLC. To our knowledge, this was the first example of MIPs using AAD-DDA hydrogen bonds.
authors.affiliation: School of Chemistry & Environment, South China Normal University, Guangzhou 510006, China.
authors.forename: Suqin
authors.initials: S
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
authors.forename: Ganquan
authors.initials: G
authors.lastname: Wang
authors.forename: Guiming
authors.initials: G
authors.lastname: Peng
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Kang
authors.forename: Youwen
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000212:Acyclovir / Q000097:blood* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006860:Hydrogen Bonding; D057230:Limit of Detection; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D011108:Polymers; D000212:Acyclovir