title: Soluble soy protein peptic hydrolysate stimulates adipocyte differentiation in 3T3-L1 cells.
abstract: METHODS
  The molecular mechanisms underlying the potential health benefit effects of soybean proteins on obesity-associated metabolic disorders have not been fully clarified. In this study, we investigated the effects of soluble soybean protein peptic hydrolysate (SPH) on adipocyte differentiation by using 3T3-L1 murine preadipocytes.
  
  
  RESULTS
  The addition of SPH increased lipid accumulation during adipocyte differentiation. SPH increased the mRNA expression levels of an adipogenic marker gene and decreased that of a preadipocyte marker gene, suggesting that SPH promotes adipocyte differentiation. SPH induced antidiabetic and antiatherogenic adiponectin mRNA expression and secretion. Moreover, SPH increased the mRNA expression levels of insulin-responsive glucose transporter 4 and insulin-stimulated glucose uptake. The expression levels of peroxisome proliferator-activated receptor γ (PPARγ), a key regulator of adipocyte differentiation, during adipocyte differentiation were up-regulated in 3T3-L1 cells treated with SPH, and lipid accumulation during adipocyte differentiation induced by SPH was inhibited in the presence of a PPARγ antagonist. However, SPH did not exhibit PPARγ ligand activity.
  
  
  CONCLUSIONS
  These findings indicate that SPH stimulates adipocyte differentiation, at least in part, via the up-regulation of PPARγ expression levels. These effects of SPH might be important for the health benefit effects of soybean proteins on obesity-associated metabolic disorders.
authors.affiliation: Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.forename: Ayaka
authors.initials: A
authors.lastname: Mori
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagaoka
mesh_terms: D041721:3T3-L1 Cells / Q000187:drug effects / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000813:Anilides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers; D051275:Glucose Transporter Type 4 / Q000235:genetics; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000235:genetics; D011492:Protein Hydrolysates / Q000494:pharmacology*; D012995:Solubility; D030262:Soybean Proteins / Q000494:pharmacology*; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D052242:Adiponectin; C499919:Adipoq protein, mouse; D000813:Anilides; D005819:Genetic Markers; D051275:Glucose Transporter Type 4; D047495:PPAR gamma; D011492:Protein Hydrolysates; C495693:Slc2a4 protein, mouse; D030262:Soybean Proteins; D045162:Thiazolidinediones; C089946:2,4-thiazolidinedione
keywords: Adipocyte differentiation; Adiponectin; Obesity; Soy peptides; Soybean protein peptic hydrolysate