title: Feasibility of circulating miRNA microarray analysis from archival plasma samples.
abstract: MicroRNAs have been found to be deregulated in several diseases and, due to their high stability in body fluids, represent promising noninvasively detectable biomarkers. However, numerous technical variables can affect accurate measurement of circulating miRNAs. Using a microarray-based method we assessed the: (i) adequate intra- and inter-array reproducibility of miRNA profiling; (ii) feasibility of using archival plasma samples stored for an extended period of time and available in limited amounts; (iii) good correlation between different batches; and (iv) time-dependent increase of background signals close to the chip expiration date.
authors.affiliation: Unit of Biomarkers, Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Callari
authors.forename: Paola
authors.initials: P
authors.lastname: Tiberio
authors.forename: Loris
authors.initials: L
authors.lastname: De Cecco
authors.forename: Elena
authors.initials: E
authors.lastname: Cavadini
authors.forename: Matteo
authors.initials: M
authors.lastname: Dugo
authors.forename: Chiara
authors.initials: C
authors.lastname: Ghimenti
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Daidone
authors.forename: Silvana
authors.initials: S
authors.lastname: Canevari
authors.forename: Valentina
authors.initials: V
authors.lastname: Appierto
mesh_terms: D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D014021:Tissue Preservation; D059467:Transcriptome
chemical_list: D035683:MicroRNAs