title: Determination of selenium using atomically imprinted polymer (AIP) and hydride generation atomic absorption spectrometry.
abstract: This paper describes selenium determination based on Se(0) preconcentration in the imprinted polymer (synthesized with 2.25mmol SeO2, 4-vinylpyridine and 1-vinylimidazole) with subsequent detection on-line in HG-FAAS. During the synthesis, SeO2 is reduced to Se (0). Therefore, there are no MIP neither IIP in the present work, thus we denominated: AIP, i.e., atomically imprinted polymers. For the optimization of analytical parameters Doehlert design was used. The method presented limit of detection and limit of quantification of 53 and 177ngL(-1), respectively, and linear range from 0.17 up to 6μgL(-1) (r=0.9936). The preconcentration factor (PF), consumptive index (CI) and concentration efficiency (CE) were 232; 0.06mL and 58min(-1) respectively. The proposed method was successfully applied to determine Se in Brazil nuts (0.33±0.03mgkg(-1)), apricot (0.46±0.02mgkg(-1)), white bean (0.47±0.03mgkg(-1)), rice flour (0.47±0.02mgkg(-1)) and milk powder (0.22±0.01mgkg(-1)) samples. It was possible to do 12 analyzes per hour. Accuracy was checked and confirmed by analyzing certified reference material (DORM-2, dogfish muscle), and samples precision was satisfactory with RSD lower than 10%.
authors.affiliation: Universidade Federal de Alfenas (UNIFAL-MG), Instituto de Química, Alfenas-MG, Brazil.
authors.forename: Grazielle Cabral
authors.initials: GC
authors.lastname: de Lima
authors.forename: Ayla Campos
authors.initials: AC
authors.lastname: do Lago
authors.forename: Arley Alves
authors.initials: AA
authors.lastname: Chaves
authors.forename: Pedro Sergio
authors.initials: PS
authors.lastname: Fadini
authors.forename: Pedro Orival
authors.initials: PO
authors.lastname: Luccas
mesh_terms: D000818:Animals; D005399:Fishes; D005504:Food Analysis; D007093:Imidazoles / Q000737:chemistry; D054802:Molecular Imprinting; D009132:Muscles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D012643:Selenium / Q000032:analysis*; D013054:Spectrophotometry, Atomic
chemical_list: D007093:Imidazoles; D011108:Polymers; D011725:Pyridines; D012643:Selenium; C029351:4-vinylpyridine; C425704:N-vinylimidazole