title: Detection of inflammation and oxidative lung injury in exhaled breath condensate of rats with acute lung injury due to Staphylococcal enterotoxin B.
abstract: The objectives were to determine if tumor necrosis factor (TNF)-α, leukotriene (LT)B4 and 8-isoprostane (IP) were present in the exhaled breath condensate (EBC) and total lung lavage (TLL) of mechanically ventilated rats, 4 and 24 h after administration of Staphylococcal entertoxin (SEB) and to find out if these mediators in the EBC correlate with the concentration in the TLL. Rats were assigned to control (n = 8); 4 h (n = 8) or 24 h (n = 8) groups after SEB. The rats were mechanically ventilated and EBC and TLL were collected for TNF-α, LTB4 and 8-IP. TNF-α was higher in the EBC of rats at 24 h after SEB when compared to control [34.5 (16-62 versus 2 (14-30) pg ml⁻¹, p < 0.05] and also in the TLL [113.5 (70-460) versus 59 (35-79) pg ml⁻¹, p < 0.04]. LTB4 was higher in the EBC of 24 h SEB rats, when compared to control [42 (28-50) versus 36 (29-37) pg ml⁻¹, p < 0.01] and also in the TLL [179 (116-232) versus 114 (80-187) pg ml⁻¹, p < 0.05). 8-IP was similar among the groups. No correlation was observed between TNF-α, LTB4 or 8-IP in the EBC compared to the TLL. TNF-α and LTB4 may be indicators of inflammation and oxidative lung injury but the EBC does not correlate with TLL concentrations.
authors.affiliation: School of Medicine, Wayne State University, Detroit, MI 48201, USA.
authors.forename: Rekha
authors.initials: R
authors.lastname: Solomon
authors.forename: Hitesh
authors.initials: H
authors.lastname: Sandhu
authors.forename: Suwannee
authors.initials: S
authors.lastname: Phumeetham
authors.forename: Keshava Murthy Narayana
authors.initials: KM
authors.lastname: Gowda
authors.forename: Sabrina M
authors.initials: SM
authors.lastname: Heidemann
mesh_terms: D055371:Acute Lung Injury / Q000175:diagnosis* / Q000378:metabolism / Q000382:microbiology; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D001944:Breath Tests / Q000379:methods*; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000633:toxicity; D045853:Exhalation; D005740:Gases / Q000737:chemistry*; D007249:Inflammation / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D004768:Enterotoxins; D005740:Gases; C031818:enterotoxin B, staphylococcal