title: Whey protein isolate edible films with essential oils incorporated to improve the microbial quality of poultry.
abstract: BACKGROUND
  Whey protein isolate edible films with oregano or clove essential oils (EOs) incorporated as natural antimicrobials have been developed, with the aim of enhancing the microbial quality of poultry. The effectiveness of the films was determined against both the whole and selected microbiota developed during different periods of cold storage on the surface of skinless chicken breast. Tests were conducted by using both turbidimetric and agar disc diffusion methods.
  
  
  RESULTS
  The antimicrobial edible films developed showed high effectiveness against the main spoilers developed on the surface of skinless chicken breasts cold-stored for 8 days. The films based on oregano EO showed greater effectiveness than those based on clove EO. Still, clove EO could be part of an effective antimicrobial edible film. Enterobacteriaceae was the most susceptible to the effect of the films when lower concentrations of EO were incorporated. The largest inhibition surfaces obtained were provoked by films with the highest concentration of oregano EO incorporated against lactic acid bacteria.
  
  
  CONCLUSIONS
  The antimicrobial edible films developed in this study inhibited the growth of the microbial populations that developed through storage of the chicken breast and caused its spoilage. The results of this research have direct application in the food industry to enhance the control of the development of spoilers such as Pseudomonas spp. or lactic acid bacteria.
authors.affiliation: Food Technology Department, Universidad Pública de Navarra, Campus Arrosadía, s/n, 31006, Pamplona, Spain.
authors.forename: Idoya
authors.initials: I
authors.lastname: Fernández-Pan
authors.forename: Mauricio
authors.initials: M
authors.lastname: Mendoza
authors.forename: Juan I
authors.initials: JI
authors.lastname: Maté
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002645:Chickens / Q000382:microbiology*; D052978:Disk Diffusion Antimicrobial Tests; D004755:Enterobacteriaceae / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D005511:Food Handling; D018857:Food Packaging; D005519:Food Preservation; D063427:Food Quality; D056546:Lactobacillales / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D008460:Meat / Q000382:microbiology*; D050296:Microbial Viability / Q000187:drug effects; D008894:Milk Proteins / Q000737:chemistry* / Q000378:metabolism; D009822:Oils, Volatile / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D031341:Origanum / Q000737:chemistry; D010369:Pectoralis Muscles / Q000382:microbiology; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D012034:Refrigeration; D013030:Spain; D013499:Surface Properties; D027842:Syzygium / Q000737:chemistry; D000067816:Whey Proteins
chemical_list: D000900:Anti-Bacterial Agents; D008894:Milk Proteins; D009822:Oils, Volatile; D000067816:Whey Proteins
keywords: antimicrobial edible film; essential oil; poultry; spoilers; whey protein isolate