title: [DING proteins, their biochemical and structural properties, and their ability to inhibit HIV-1 replication].
abstract: DING proteins comprise an intriguing phosphate-binding protein family present in all animal phyla. Five different DING representatives have been described in humans. Eukaryotic DING proteins are mostly involved in cellular processes such as cell cycle regulation, and also in pathological process such as rheumatoid arthritis and kidney stone formation. Although these proteins are ubiquitous in eukaryotes, no relevant locus or ORF has yet been found in sequenced genomes. This lack of sequence information has considerably hampered functional and structural studies of these proteins, and has required the use of novel and original techniques such as ab initio protein sequencing based on a combination of X-ray crystallography and mass spectrometry. Sub-Angstrom structural resolution has elucidated the molecular binding mechanism of phosphate ions by these high-affinity proteins. Immunohistochemical studies show that these proteins are present in a wide variety of mouse tissues. Some DING proteins, particularly human phosphate binding protein (HPBP), can inhibit HIV replication. This inhibition takes place at the transcriptional step, which is not targeted by any current antiretroviral drug. Initial studies suggest that HPBP warrants animal testing. This recent discovery opens new possibilities for the treatment of HIV infection.
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE-UMR CNRS 7228, IRD 198, INSERM U1095, Université de la Méditerranée (Aix-Marseille II), 27 bid Jean Moulin, 13 385 Marseille, France. eric.chabriere@univmed.fr
authors.forename: Eric
authors.initials: E
authors.lastname: Chabriere
authors.forename: Mikael
authors.initials: M
authors.lastname: Elias
authors.forename: Julien
authors.initials: J
authors.lastname: Hiblot
authors.forename: Ahmed
authors.initials: A
authors.lastname: Djeghader
authors.forename: Christian
authors.initials: C
authors.lastname: Schwartz
authors.forename: Olivier
authors.initials: O
authors.lastname: Rohr
authors.forename: Patrick
authors.initials: P
authors.lastname: Masson
mesh_terms: D015497:HIV-1 / Q000502:physiology*; D028044:Phosphate-Binding Proteins / Q000502:physiology*; D017434:Protein Structure, Tertiary; D014779:Virus Replication / Q000502:physiology*
chemical_list: D028044:Phosphate-Binding Proteins