title: Development of a disperse dye immunoassay technique for detection of antibodies against Neospora caninum in cattle.
abstract: In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.
authors.affiliation: Islamic Azad University of Jahrom, Jahrom, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Selahi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Namavari
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Hosseini
authors.forename: Maryam
authors.initials: M
authors.lastname: Mansourian
authors.forename: Yahya
authors.initials: Y
authors.lastname: Tahamtan
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D007118:Immunoassay / Q000379:methods; D018688:Neospora / Q000276:immunology*; D013194:Staining and Labeling / Q000379:methods; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000913:Antibodies, Protozoan
keywords: Cattle; Colloidal dye; Dot dye immunoassay; Neospora caninum