title: [Evaluation of the immunochromatography kit for detection of emetic-toxin producing Bacillus cereus].
abstract: We evaluated the sensitivity and specificity of an immunochromatography kit, Single-path Emetic Tox Mrk (Merck), which targets a marker protein for the detection of Bacillus cereus that produces emetic toxin. Strains were isolated after outbreaks of food poisoning, and from retail prepared foods and food products. The strains were examined for the presence of the emetic toxin-synthetase gene by PCR. All 58 emetic strains isolated from the food poisoning cases showed a positive reaction in the immunochromatography kit. No emetic strains gave false negative result. Among 47 non-emetic strains, only two strains isolated from the food poisonings and one strain isolated from food products showed a false positive reaction in the test. We concluded that this method has high sensitivity and specificity. The test can be used for detection of emetic toxin-producing B. cereus not only from food poisoning cases, but also in food products.
authors.affiliation: Kao Corporation, 2606 Akabane, Tochigi, Japan.
authors.forename: Motokazu
authors.initials: M
authors.lastname: Nakayama
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyashita
authors.forename: Kouichi
authors.initials: K
authors.lastname: Hosoya
authors.forename: Jun
authors.initials: J
authors.lastname: Hitomi
authors.forename: Miki
authors.initials: M
authors.lastname: Sato
authors.forename: Yukie
authors.initials: Y
authors.lastname: Sunaga
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shigematsu
authors.forename: Jun
authors.initials: J
authors.lastname: Ogasawara
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Takenaka
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hamasaki
authors.forename: Kazumi
authors.initials: K
authors.lastname: Horikawa
authors.forename: Junko
authors.initials: J
authors.lastname: Isobe
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamata
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D047630:Depsipeptides / Q000096:biosynthesis*; D004196:Disease Outbreaks; D005188:False Negative Reactions; D005189:False Positive Reactions; D005516:Food Microbiology / Q000379:methods*; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D010453:Peptide Synthases / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity
chemical_list: D047630:Depsipeptides; D011933:Reagent Kits, Diagnostic; C089294:cereulide; D010453:Peptide Synthases