title: MiR-145 functions as a tumor suppressor by directly targeting histone deacetylase 2 in liver cancer.
abstract: Aberrant regulation of histone deacetylase 2 (HDAC2) plays a pivotal role in the development of hepatocellular carcinoma (HCC), but, the underlying mechanism leading to HDAC2 overexpression is not well understood. We performed microRNA (miRNA) profiling analysis in a subset of HCCs, and identified four down-regulated miRNAs that may target HDAC2 in HCC. Ectopic expression of miRNA mimics evidenced that miR-145 suppresses HDAC2 expression in HCC cells. This treatment repressed cancer cell growth and recapitulated HDAC2 knockdown effects on HCC cells. In conclusion, we suggest that loss or suppression of miR-145 may cause aberrant overexpression of HDAC2 and promote HCC tumorigenesis.
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Ji Heon
authors.initials: JH
authors.lastname: Noh
authors.forename: Young Gyoon
authors.initials: YG
authors.lastname: Chang
authors.forename: Min Gyu
authors.initials: MG
authors.lastname: Kim
authors.forename: Kwang Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Jeong Kyu
authors.initials: JK
authors.lastname: Kim
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Bae
authors.forename: Jung Woo
authors.initials: JW
authors.lastname: Eun
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Shen
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: So Hee
authors.initials: SH
authors.lastname: Kwon
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Nam
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016147:Genes, Tumor Suppressor; D056464:Histone Deacetylase 2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009368:Neoplasm Transplantation; D034622:RNA Interference; D034741:RNA, Small Interfering; D023041:Xenograft Model Antitumor Assays
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C533423:HDAC2 protein, human; D056464:Histone Deacetylase 2