title: Engineering and application of collagen-binding fibroblast growth factor 2 for sustained release.
abstract: The sustained release of growth factors plays a critical role in therapeutic applications because of the instability of these factors in the body. Here, we designed a fibroblast growth factor 2 (FGF2) fused with a collagen-binding domain (rhCBD-FGF2) for collagen-based sustained release of FGF2.The release profile of rhCBD-FGF2 showed sustained release from collagen matrices. Further, rhCBD-FGF2 also stimulated adhesion of the MC3T3-E1 cells to the collagen matrices. In addition, rhCBD-FGF2 increased the cell proliferation activity at 3 and 5 days in the MC3T3-E1 cells attached to the collagen matrices compared to that in the control. Further, rhCBD-FGF2 significantly induced the osteogenic differentiation of MC3T3-E1 cells on collagen matrices by up-regulating the alkaline phosphatase activity at 7 days. These osteogenic differentiation activities were confirmed in gene expression of MC3T3-E1 cell. Taken together, rhCBD-FGF2 could specifically bind with collagen matrices, which indicates important advancements in bone tissue engineering.
authors.affiliation: Department of Biochemistry, Inha University School of Medicine, Incheon, 400-712, Korea.
authors.forename: Eunyi
authors.initials: E
authors.lastname: Jeon
authors.forename: Ye-Rang
authors.initials: YR
authors.lastname: Yun
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology*
chemical_list: D003692:Delayed-Action Preparations; D011993:Recombinant Fusion Proteins; D016222:Fibroblast Growth Factor 2; D003094:Collagen
keywords: bone tissue engineering; cell differentiation; cell proliferation; collagen matrices; rhCBD‐FGF2