title: Characterization and mapping of a salt-sensitive mutant in rice (Oryza sativa L.).
abstract: A salt-sensitive mutant designated rice salt sensitive 2 (rss2) was isolated from the M2 generation of the rice cultivar Nipponbare mutagenized with ethyl methanesulfonate (EMS). This mutant exhibited a greater decrease in salt tolerance with a significant increase in Na(+) content in its shoots. Genetic analysis indicated that the increase in Na(+) in rss2 was controlled by a single recessive gene. Further genome-wide analysis of the linkage map constructed from the F2 population of rss2/Zhaiyeqing 8 (ZYQ8) showed that two quantitative trait loci (QTLs) on chromosomes 1 and 6 were responsible for the Na(+) concentration in shoots, which explained 14.5% and 53.3%, respectively, of the phenotypic variance. The locus on chromosome 1, but not that on chromosome 6, was also detected in the F2 population of Nipponbare/ZYQ8, suggesting that the QTL on chromosome 6 was responsible for the salt sensitivity in rss2. By analyzing the recombination events in 220 mutant individuals of an enlarged mapping population of rss2/ZYQ8, the rss2 locus was precisely mapped to an interval of 605.3 kb between insertion/deletion (InDel) markers IM21962 and IM22567. This finding will facilitate the cloning of the rss2 locus and provide insight into the physiological mechanisms of salt sensitivity in rice.
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. jingwen@njau.edu.cn
authors.forename: Jingwei
authors.initials: J
authors.lastname: Zhou
authors.forename: Fuzheng
authors.initials: F
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Deng
authors.forename: Wen
authors.initials: W
authors.lastname: Jing
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D040641:Quantitative Trait Loci / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride