title: Diffusion-controlled detection of glucose with microelectrodes in mediated bioelectrocatalytic oxidation.
abstract: This paper describes a diffusion-controlled electrolysis of glucose with mediated bioelectrocatalysis at microdisk-electrodes. Under conditions of an extremely fast enzyme reaction, compared with the diffusion of glucose, the current in potential-step chronoamperometry reaches an almost steady state within 10 s, and is proportional to the glucose concentration. The current can be detected at +0.1 V (vs. Ag|AgCl) with 9,10-phenanthrenequinone as a mediator. The addition of carboxymethylcellulose increased the linear range up to 10 mM.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Noda
authors.forename: Mizue
authors.initials: M
authors.lastname: Wanibuchi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kitazumi
authors.forename: Seiya
authors.initials: S
authors.lastname: Tsujimura
authors.forename: Osamu
authors.initials: O
authors.lastname: Shirai
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Kano
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D004058:Diffusion; D004572:Electrolysis / Q000295:instrumentation*; D004579:Electron Transport; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D008839:Microelectrodes; D010616:Phenanthrenes / Q000737:chemistry
chemical_list: D010616:Phenanthrenes; D005182:Flavin-Adenine Dinucleotide; C005399:9,10-phenanthrenequinone; D042843:Glucose 1-Dehydrogenase; D005947:Glucose; D002266:Carboxymethylcellulose Sodium