title: Biochemical and biophysical properties of a putative hub protein expressed by vaccinia virus.
abstract: H5 is a constitutively expressed, phosphorylated vaccinia virus protein that has been implicated in viral DNA replication, post-replicative gene expression, and virus assembly. For the purpose of understanding the role of H5 in vaccinia biology, we have characterized its biochemical and biophysical properties. Previously, we have demonstrated that H5 is associated with an endoribonucleolytic activity. In this study, we have shown that this cleavage results in a 3'-OH end suitable for polyadenylation of the nascent transcript, corroborating a role for H5 in vaccinia transcription termination. Furthermore, we have shown that H5 is intrinsically disordered, with an elongated rod-shaped structure that preferentially binds double-stranded nucleic acids in a sequence nonspecific manner. The dynamic phosphorylation status of H5 influences this structure and has implications for the role of H5 in multiple processes during virus replication.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida 32610-0266, USA.
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Kay
authors.forename: Travis W
authors.initials: TW
authors.lastname: Bainbridge
authors.forename: Richard C
authors.initials: RC
authors.lastname: Condit
authors.forename: Michael R
authors.initials: MR
authors.lastname: Bubb
authors.forename: Reuben E
authors.initials: RE
authors.lastname: Judd
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Venkatakrishnan
authors.forename: Robert
authors.initials: R
authors.lastname: McKenna
authors.forename: Susan M
authors.initials: SM
authors.lastname: D'Costa
mesh_terms: D004261:DNA Replication / Q000502:physiology; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation / Q000502:physiology; D017434:Protein Structure, Tertiary; D061806:Transcription Termination, Genetic / Q000502:physiology*; D014615:Vaccinia / Q000235:genetics / Q000378:metabolism; D014616:Vaccinia virus / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004722:Endoribonucleases