title: Marrow-derived mesenchymal stem cells restore biochemical markers of acute liver injury in experimental model.
abstract: Bone marrow-derived mesenchymal stem cells were investigated as prompters of liver regeneration in an experimental model of acute hepatic injury. A model was created in Wistar rats through intraperitoneal injection of carbon tetrachloride (CCl4). Bone marrow-derived mesenchymal stem cells collected from the long bones of 10 Wistar rats were intravenously infused 24 hours after induction of acute liver failure in 16 rats, group A. In group B, the control group, 16 rats received a peritoneal injection of CCl4, and an intravenous infusion of normal saline solution. All rats were sacrificed at 2, 3, 4, and 7 days post-CCl4 injection to examined biochemical markers and pathological appearances. The platelet counts were higher in group A versus group B on post-CCl4 infusion days 2 (P = .02) and 3 (P = .001), as were the transaminase trends in glutamic oxaloacetic (P = .002), and glutamic-pyruvic transaminases (P < .0001). Pathological examination showed a greater grade of hepatocellular necrosis with neutrophilic infiltration in group B (P = .02). In conclusion, infusion of bone marrow-derived mesenchymal stem cell resulted in a less aggressive picture of hepatic damage.
authors.affiliation: Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione, University of Pittsburgh Medical Center in Italy, Palermo, Italy. sgruttadauria@ismett.edu
authors.forename: S
authors.initials: S
authors.lastname: Gruttadauria
authors.forename: G
authors.initials: G
authors.lastname: Grosso
authors.forename: D
authors.initials: D
authors.lastname: Pagano
authors.forename: A
authors.initials: A
authors.lastname: Biondi
authors.forename: G J
authors.initials: GJ
authors.lastname: Echeverri
authors.forename: E
authors.initials: E
authors.lastname: Seria
authors.forename: G
authors.initials: G
authors.lastname: Pietrosi
authors.forename: R
authors.initials: R
authors.lastname: Liotta
authors.forename: F
authors.initials: F
authors.lastname: Basile
authors.forename: B
authors.initials: B
authors.lastname: Gridelli
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016026:Bone Marrow Transplantation; D002251:Carbon Tetrachloride; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D019934:International Normalized Ratio; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008115:Liver Regeneration; D045164:Mesenchymal Stem Cell Transplantation; D009336:Necrosis; D020556:Neutrophil Infiltration; D010641:Phenotype; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D013997:Time Factors
chemical_list: D015415:Biomarkers; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase