title: Heterogeneous differentiation patterns of individual CD8+ T cells.
abstract: Upon infection, antigen-specific CD8(+) T lymphocyte responses display a highly reproducible pattern of expansion and contraction that is thought to reflect a uniform behavior of individual cells. We tracked the progeny of individual mouse CD8(+) T cells by in vivo lineage tracing and demonstrated that, even for T cells bearing identical T cell receptors, both clonal expansion and differentiation patterns are heterogeneous. As a consequence, individual naïve T lymphocytes contributed differentially to short- and long-term protection, as revealed by participation of their progeny during primary versus recall infections. The discordance in fate of individual naïve T cells argues against asymmetric division as a singular driver of CD8(+) T cell heterogeneity and demonstrates that reproducibility of CD8(+) T cell responses is achieved through population averaging.
authors.affiliation: Division of Immunology, Netherlands Cancer Institute, Amsterdam, Netherlands.
authors.forename: Carmen
authors.initials: C
authors.lastname: Gerlach
authors.forename: Jan C
authors.initials: JC
authors.lastname: Rohr
authors.forename: Leïla
authors.initials: L
authors.lastname: Perié
authors.forename: Nienke
authors.initials: N
authors.lastname: van Rooij
authors.forename: Jeroen W J
authors.initials: JW
authors.lastname: van Heijst
authors.forename: Arno
authors.initials: A
authors.lastname: Velds
authors.forename: Jos
authors.initials: J
authors.lastname: Urbanus
authors.forename: Shalin H
authors.initials: SH
authors.lastname: Naik
authors.forename: Heinz
authors.initials: H
authors.lastname: Jacobs
authors.forename: Joost B
authors.initials: JB
authors.lastname: Beltman
authors.forename: Rob J
authors.initials: RJ
authors.lastname: de Boer
authors.forename: Ton N M
authors.initials: TN
authors.lastname: Schumacher
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D060049:Asymmetric Cell Division; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D007111:Immunity, Cellular; D007156:Immunologic Memory; D016130:Immunophenotyping; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018448:Models, Immunological; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D059010:Single-Cell Analysis; D013269:Stochastic Processes; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell