title: Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation.
abstract: Cartilage degeneration with osteoarthritis (OA) is believed to involve the activities of interleukin-1 (IL-1), which exists as alpha and beta isoforms. The goal of this study was to measure the concentrations of both isoforms of IL-1 in the synovial fluid of normal and spontaneously osteoarthritic porcine knees, and to test the hypothesis that physiologic concentrations of IL-1α and IL-1β exhibit different potencies in activating calcium signaling, the production of matrix metalloproteinases and nitric oxide, and the loss of proteoglycans and tissue mechanical properties in cartilage and meniscus. Median concentrations of IL-1α were 0.043 ng/ml with mild OA and 0.288 ng/ml with moderate OA, whereas IL-1β concentrations were 0.109 ng/ml with mild OA and 0.122 ng/ml with moderate OA. Both isoforms induced calcium signaling in chondrocytes and meniscal cells at all concentrations. Overall, cartilage and meniscus catabolism was significantly more sensitive to IL-1α than IL-1β at concentrations of 1 ng/ml or less, while few differences were observed between the two forms at 10 ng/ml. These data provide a range of physiologic IL-1 concentrations that can serve as a framework for the comparison of various in vitro studies, as well as providing further insight for the development of anti-cytokine therapies for OA.
authors.affiliation: Department of Orthopaedic Surgery, Duke University Medical Center, Box 3093, Durham, North Carolina 27710, USA.
authors.forename: Amy L
authors.initials: AL
authors.lastname: McNulty
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Rothfusz
authors.forename: Holly A
authors.initials: HA
authors.lastname: Leddy
authors.forename: Farshid
authors.initials: F
authors.lastname: Guilak
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D020013:Calcium Signaling / Q000502:physiology; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D053582:Interleukin-1alpha / Q000378:metabolism* / Q000494:pharmacology; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology; D020782:Matrix Metalloproteinases / Q000378:metabolism; D008592:Menisci, Tibial / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020370:Osteoarthritis, Knee / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism; D012720:Severity of Illness Index; D013552:Swine; D013582:Synovial Fluid / Q000378:metabolism*
chemical_list: D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D011509:Proteoglycans; D020782:Matrix Metalloproteinases