title: Expression of a vitelline membrane protein, BmVMP23, is repressed by bmo-miR-1a-3p in silkworm, Bombyx mori.
abstract: Previous studies have shown that the 3' UTR of BmVMP23 was destroyed by an inserted fragment, and the BmVMP23 expression was downregulated in the lethal egg of "Ming" (l-e(m)). In this study, we found a miRNA (bmo-miR-1a-3p) that matches the 3' UTR sequence of BmVMP23 perfectly. The relationship between bmo-miR-1a-3p and the BmVMP23 expression was examined by the co-transfection in vitro. The luciferase assay result showed that luc expression was strongly repressed. This result inferred that bmo-miR-1a-3p may downregulate BmVMP23 expression via complementary interaction with the target sites at the 3' UTR.
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China.
authors.forename: Anli
authors.initials: A
authors.lastname: Chen
authors.forename: Dingguo
authors.initials: D
authors.lastname: Xia
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qiu
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Shunming
authors.initials: S
authors.lastname: Tang
authors.forename: Xingjia
authors.initials: X
authors.lastname: Shen
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004527:Egg Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; D004527:Egg Proteins; D019476:Insect Proteins; D035683:MicroRNAs; C035651:vitelline membrane proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases