title: [Expression of Btk and NFκB in acute myeloid leukemia cells and its significance].
abstract: This study was purposed to investigate the expression of Btk and NFκB in acute myeloid leukemia (AML) cells and its significance. Bone marrow mononuclear cell specimens were taken from 14 AML patients who were in new diagnosis and complete remission respectively, the expressions of Btk and NFκB at mRNA and protein levels were detected by RT-PCR and Western blot, respectively. The results showed that Btk and NFκB expressed in all the samples at RNA and protein levels. At protein level, Btk and NFκB expressions were higher in the cells from newly diagnosed AML patients than that in the cells from patients in complete remission stage (P < 0.05). It is concluded that Btk and NFκB may play an important role in the development and progression of AML, they may be used as potential therapeutic targets of AML and used in predicting the prognosis.
authors.affiliation: Department of Hematology, Nanjing Medical University, Huai'an, Jiangsu Province, China.
authors.forename: Shan-Dong
authors.initials: SD
authors.lastname: Tao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Deng
authors.forename: Zheng-Mei
authors.initials: ZM
authors.lastname: He
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Deng
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Jia-Bin
authors.initials: JB
authors.lastname: Zhu
authors.forename: Bang-He
authors.initials: BH
authors.lastname: Ding
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
mesh_terms: D000293:Adolescent; D000328:Adult; D000077329:Agammaglobulinaemia Tyrosine Kinase; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics*; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625949:BTK protein, human