title: [Research of the synergetic action of fluid shear stress and 17-beta estradiol on the proliferation of MC3T3-E1 cells].
abstract: OBJECTIVE
  To explore the suitable level and action time of 17-beta estradiol and fluid shear stress (FSS) and their combined effect on the proliferation of rat osteoblasts in vitro.
  
  
  METHODS
  MC3T3-E1 osteoblasts were adopted after subcultured and different concentrations of 17-beta estradiol and FSS values were applied respectively on MC3T3-E1, the suitable level of 17-beta estradiol and FSS were selected through MTT and alkaline phosphatase (ALP). Then the two factors at the suitable level were applied simultaneously to MC3T3-E1 to detect the proliferation activity.
  
  
  RESULTS
  Seventeen-beta estradiol(10(-8) mol x L(-1) for 5 d and 12 x 10(-5) N FSS for 60 min exhibited better effects on the proliferation activity than the other groups respectively, and the combined effect of both factors was better than any single-factor treated group.
  
  
  CONCLUSIONS
  Both 17-beta estradiol and FSS have a suitable threshold in promoting proliferation of osteoblasts, and two-factor treated group exhibits better effect than any other single-factor treated groups. Therefore 17-beta estradiol and FSS have a synergetic action on differentiation and proliferation of osteoblasts.
authors.affiliation: Dept. of Prosthodontics, College of Stomatology, Shandong University, Jinan 250012, China.
authors.forename: Sixia
authors.initials: S
authors.lastname: Shang
authors.forename: Linlin
authors.initials: L
authors.lastname: Yin
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Sun
authors.forename: Keli
authors.initials: K
authors.lastname: Jia
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D002454:Cell Differentiation; D004958:Estradiol; D010006:Osteoblasts; D051381:Rats; D013314:Stress, Mechanical
chemical_list: D004958:Estradiol; D000469:Alkaline Phosphatase