title: Synergistic effects of low-level laser and mesenchymal stem cells on functional recovery in rats with crushed sciatic nerves.
abstract: Transplantation of mesenchymal stem cells (MSCs) has been proposed to exert beneficial effects on peripheral nerve regeneration after a peripheral nerve injury, but the functional recovery in the denervated limb is still limited. In this study, we used low-level laser therapy (LLLT) as an adjunct therapy for MSC transplantation on the functional recovery of crushed sciatic nerve in rats. Peripheral nerve injury was induced in 48 Sprague-Dawley rats by crushing the unilateral sciatic nerve, using a vessel clamp. The animals with crushed injury were randomly divided into four groups: control group, with no treatment; MSC group, treated with MSC alone; LLLT group, treated with LLLT alone; and MSCLLLT group, treated with a combination of MSC and LLLT. The sciatic function index (SFI), vertical activity of locomotion (VA) and ankle angle (AA) of rats were examined for functional assessments after treatment. Electrophysiological, morphological and S100 immunohistochemical studies were also conducted. The MSCLLLT group showed a greater recovery in SFI, VA and AA, with significant difference from MSC, LLLT and control groups (p < 0.05). Moreover, markedly enhanced electrophysiological function and expression of S100 immunoreactivity, as well as fewer inflammatory cells and less vacuole formation were also demonstrated after nerve crush injury in the MSCLLLT group when compared with the groups receiving a single treatment (p < 0.05). MSC transplantation combined with LLLT could achieve better results in functional recovery than a conventional treatment of MSC or LLLT alone. LLLT has a synergistic effect in providing greater functional recovery with MSC transplantation after nerve crush injury.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Taichung Tzu Chi General Hospital, Taiwan.
authors.forename: Chen-Chia
authors.initials: CC
authors.lastname: Yang
authors.affiliation: Department of Pathology and Laboratory Medicine, Taichung Veterans General Hospital, Taiwan.
authors.forename: John
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology and Laboratory Medicine, Taichung Veterans General Hospital, Taiwan.
authors.forename: Shyh-Chang
authors.initials: SC
authors.lastname: Chen
authors.affiliation: Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan.
authors.forename: Yueh-Ling
authors.initials: YL
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D007249:Inflammation / Q000473:pathology; D008124:Locomotion; D028022:Low-Level Light Therapy; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D009409:Nerve Crush; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012584:Sciatic Nerve / Q000473:pathology* / Q000503:physiopathology*
keywords: functional recovery; low-level laser therapy; mesenchymal stem cell; nerve crush injury; nerve regeneration; synergistic effect