title: Characterization and activity of anthocyanins in Zijuan tea (Camellia sinensis var. kitamura).
abstract: Zijuan tea is a new cultivar produced in Yunnan province of China. Unlike most tea cultivars, Zijuan tea is anthocyanin-rich. The composition and antioxidant activities of anthocyanins of Zijuan tea were studied for the first time in this paper. Anthocyanins were extracted with acidified methanol and quantified as 707 ± 28 μg/g of dry weight (cyanidin-3-O-β-D-glucoside equivalent) by high-performance liquid chromatography (HPLC) analysis. Four anthocyanins were successfully identified after Amberlite XAD-7HP adsorption column chromatography and octadecyl silane (ODS) flash chromatography. Among the four, delphinidin-3-O-β-D-galactoside (1) and cyanidin-3-O-β-D-galactoside (2) were confirmed by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) and HPLC. Delphinidin-3-O-β-D-(6-(E)-p-coumaroyl) galactopyranoside (3) and cyanidin-3-O-β-D-(6-(E)-p-coumaroyl) galactopyranoside (4) were characterized by the high-resolution time-of-flight-mass spectrometry (HRTOF-MS) and nuclear magnetic resonance (NMR) spectrometry. The antioxidant activities of compounds 3 and 4, which composed approximately 75% of the total anthocyanins content in HPLC analysis, were evaluated by DPPH and FRAP assays. Results showed that both had higher antioxidant activities than commercial antioxidants butylated hydroxytoluene (BHT) used as one of controls in these assays.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Shen
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Shoji
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Kanda
authors.forename: Jiachun
authors.initials: J
authors.lastname: Zhou
authors.forename: Liming
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D021241:Spectrometry, Mass, Electrospray Ionization; D013662:Tea / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D013662:Tea