title: Citrate synthase from the liver fluke Fasciola hepatica.
abstract: Citrate synthase catalyses the first step of the Krebs' tricarboxylic acid cycle. A sequence encoding citrate synthase from the common liver fluke, Fasciola hepatica, has been cloned. The encoded protein sequence is predicted to fold into a largely α-helical protein with high structural similarity to mammalian citrate synthases. Although a hexahistidine-tagged version of the protein could be expressed in Escherichia coli, it was not possible to purify it by nickel-affinity chromatography. Similar results were obtained with a version of the protein which lacks the putative mitochondrial targeting sequence (residues 1 to 29). However, extracts from bacterial cells expressing this version had additional citrate synthase activity after correcting for the endogenous, bacterial activity. The apparent K m for oxaloacetate was found to be 0.22 mM, which is higher than that observed in mammalian citrate synthases. Overall, the sequence and structure of F. hepatica citrate synthase are similar to ones from other eukaryotes, but there are enzymological differences which merit further investigation.
authors.affiliation: School of Biological Sciences, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Veronika L
authors.initials: VL
authors.lastname: Zinsser
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Moore
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Hoey
authors.forename: Alan
authors.initials: A
authors.lastname: Trudgett
authors.forename: David J
authors.initials: DJ
authors.lastname: Timson
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005210:Fasciola hepatica / Q000201:enzymology*; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D062907:Oxaloacetic Acid / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017422:Sequence Analysis, DNA
chemical_list: D018509:DNA, Helminth; D062907:Oxaloacetic Acid; D002950:Citrate (si)-Synthase