title: Alkaline polyol pulping and enzymatic hydrolysis of softwood: effect of pulping severity and pulp properties on cellulase activity and overall sugar yield.
abstract: The saccharification of softwood using alkaline polyol pulping (AlkaPolP) and enzymatic hydrolysis was investigated. It will be demonstrated that the AlkaPolP process yields high quality pulps which can easily be hydrolyzed by cellulases. Temperature (180-230°C) and duration (15-60 min) of the alkaline glycerol pulping, expressed as pulping severity R0, were varied to find optimum reaction conditions. The obtained pulps were characterized regarding their residual lignin content, kappa number and crystallinity index. Thus, the dependencies of the conversion during enzymatic hydrolysis on severity, pulp composition and pulp characteristics could be observed. In further experiments it was investigated how the enzymatic hydrolysis is affected by pulp drying or by a reduction of enzyme loading. Up to 83% of the initial cellulose in wood and almost 97% of the cellulose in pulp were converted into glucose using cellulases from Trichoderma reesei and β-glucosidase from Aspergillus niger.
authors.affiliation: Brandenburg University of Technology Cottbus, Department of Chemical Reaction Engineering, Burger Chaussee 2, 03044 Cottbus, Germany. m.hundt@rt.tu-cottbus.de
authors.forename: Martin
authors.initials: M
authors.lastname: Hundt
authors.forename: Klaus
authors.initials: K
authors.lastname: Schnitzlein
authors.forename: Michael G
authors.initials: MG
authors.lastname: Schnitzlein
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D001234:Aspergillus niger / Q000201:enzymology; D002241:Carbohydrates / Q000096:biosynthesis*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003460:Crystallization; D005990:Glycerol / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D006878:Hydroxides / Q000494:pharmacology; D008031:Lignin / Q000302:isolation & purification; D028222:Picea / Q000187:drug effects* / Q000378:metabolism; D028223:Pinus / Q000187:drug effects* / Q000378:metabolism; D011108:Polymers / Q000378:metabolism*; D017680:Potassium Compounds / Q000494:pharmacology; D014242:Trichoderma / Q000201:enzymology; D014934:Wood / Q000187:drug effects / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D000468:Alkalies; D002241:Carbohydrates; D006878:Hydroxides; D011108:Polymers; D017680:Potassium Compounds; D014990:Xylans; C024617:polyol; D002482:Cellulose; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D005990:Glycerol; C029943:potassium hydroxide