title: Antibacterial substances from Albizia myriophylla wood against cariogenic Streptococcus mutans.
abstract: Albizia myriophylla has been used for long by Thai traditional healers as an important ingredient herb in Thai herbal formulas for caries. In this study, three flavonoids lupinifolin (6), 8-methoxy-7,3',4'-trihydroxyflavone (7), and 7,8,3',4'-tetrahydroxyflavone (8), a triterpenoid lupeol (3) as well as four sterols β-sitosterone (1), stigmasta-5,22-dien-3-one (2), β-sitosterol (4), and stigmasterol (5) were isolated from A. myriophylla wood. The antibacterial activity of these compounds against Streptococcus mutans ATCC 25175 was performed using broth microdilution method. All compounds exhibited antibacterial activity against S. mutans with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ranging from 1-256 and 2-256 μg/ml, respectively. Among the isolated compounds, lupinifolin (6) was found to be the most potent with MIC and MBC of 1 and 2 μg/ml, respectively. Lupinifolin (6) also showed a strong activity against ten clinical isolates of S. mutans with MIC and MBC ranging from 0.25-2 and 0.5-8 μg/ml, respectively. These results reported the bioactive ingredients of A. myriophylla which support its ethnomedical claims as well. Lupinifolin (6) may have a potential to be a natural anticariogenic agent.
authors.affiliation: Natural Products Research Center, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand. nantiya.j@psu.ac.th
authors.forename: Nantiya
authors.initials: N
authors.lastname: Joycharat
authors.forename: Sonesay
authors.initials: S
authors.lastname: Thammavong
authors.forename: Surasak
authors.initials: S
authors.lastname: Limsuwan
authors.forename: Sirilux
authors.initials: S
authors.lastname: Homlaead
authors.forename: Supayang Piyawan
authors.initials: SP
authors.lastname: Voravuthikunchai
authors.forename: Boon-Ek
authors.initials: BE
authors.lastname: Yingyongnarongkul
authors.forename: Sukanya
authors.initials: S
authors.lastname: Dej-Adisai
authors.forename: Sanan
authors.initials: S
authors.lastname: Subhadhirasakul
mesh_terms: D031289:Albizzia / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects*; D013785:Thailand
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010936:Plant Extracts; D013261:Sterols