title: Taxonomic and functional diversity of Streptomyces in a forest soil.
abstract: In this work we report the isolation and the characterization of 79 Streptomyces isolates from a French forest soil. The 16S rRNA gene phylogeny indicated that a great diversity of Streptomyces was present in this soil, with at least nine different and potentially new species. Growth plate assays showed that most Streptomyces lineages exhibit cellulolytic and hemicellulolytic capacities and potentially participate in wood decomposition. Molecular screening for a specific hydrogenase also indicated a widespread potential for atmospheric H2 uptake. Co-culture experiments with representative strains showed antagonistic effects between Streptomyces of the same population and between Streptomyces and various fungi. Interestingly, in certain conditions, growth promotion of some fungi also occurred. We conclude that in forest soil, Streptomyces populations exhibit many important functions involved in different biogeochemical cycles and also influence the structure of soil microbial communities.
authors.affiliation: Dynamique des Génomes et Adaptation Microbienne, UMR1128, Université de Lorraine, Vandoeuvre-lès-Nancy F-54506, France. cyril.bontemps@univ-lorraine.fr
authors.forename: Cyril
authors.initials: C
authors.lastname: Bontemps
authors.forename: Maxime
authors.initials: M
authors.lastname: Toussaint
authors.forename: Pierre-Vincent
authors.initials: PV
authors.lastname: Revol
authors.forename: Laurence
authors.initials: L
authors.lastname: Hotel
authors.forename: Mathilde
authors.initials: M
authors.lastname: Jeanbille
authors.forename: Stéphane
authors.initials: S
authors.lastname: Uroz
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Turpault
authors.forename: Damien
authors.initials: D
authors.lastname: Blaudez
authors.forename: Pierre
authors.initials: P
authors.lastname: Leblond
mesh_terms: D044602:Cellulases / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005602:France; D014644:Genetic Variation; D006864:Hydrogenase / Q000235:genetics; D056265:Microbial Interactions; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014197:Trees
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D006864:Hydrogenase; D044602:Cellulases