title: Resazurin-based assay for screening bacteria for radiation sensitivity.
abstract: We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours.
authors.affiliation: Department of Microbiology and Immunology, AT Still University of Health Sciences, Kirksville, MO USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Hudman
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Sargentini
keywords: Escherichia coli; High throughput; Radiation-sensitivity assay; Resazurin; UV radiation; X-radiation