title: Tubulin beta chain, filamin A alpha isoform 1, and cytochrome b-c1 complex subunit 1 as serological diagnostic biomarkers of esophageal squamous cell carcinoma: a proteomics study.
abstract: Despite the major advances in diagnosis and treatment, esophageal squamous cell carcinoma (ESCC) remains a major life-threatening disease. Early diagnosis is critical for guiding the therapeutic management of ESCC. This case-control study focused on the proteomic analysis of serum of healthy volunteers and ESCC patients using the ClinProt profiling technology based on mass spectrometry. A total of 80 healthy volunteers and 119 ESCC patients were enrolled. We identified a pattern of proteins/peptides (including m/z 1867, 2700, and 2094) and differentiated ESCC patients from healthy volunteers with sensitivity and specificity close to 100%. Using mass spectrometry (LTQ orbitrap XL), tubulin beta chain, filamin A alpha isoform 1, and cytochrome b-c1 complex subunit 1 were identified as the three differentially expressed proteins/peptides in the patient serum. These three dysregulated proteins/peptides could be involved in the pathogenesis of ESCC and may serve as putative serological diagnostic biomarkers of ESCC. We suggest that further proteomics and multi-omics research are warranted to identify novel post-genomics diagnostics that can in the future pave the way for personalized medicine for patients with ESCC, a cancer for which we currently lack an integrated battery of diagnostics in the field of oncology.
authors.affiliation: Research Institute of Anal-colorectal Surgery, No. 150 Central Hospital of PLA, Luoyang, RP China.
authors.forename: Nai-Jun
authors.initials: NJ
authors.lastname: Fan
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Gao
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis*; D016022:Case-Control Studies; D003285:Contractile Proteins / Q000097:blood*; D045303:Cytochromes b / Q000097:blood*; D004938:Esophageal Neoplasms / Q000097:blood* / Q000175:diagnosis*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D064448:Filamins; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D020033:Protein Isoforms; D040901:Proteomics; D012372:ROC Curve; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014404:Tubulin / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D020033:Protein Isoforms; D014404:Tubulin; D045303:Cytochromes b