title: Characterization of one- and two-photon photochemical uncaging efficiency.
abstract: The idea of using light to unleash biologically active compounds from inert precursors (uncaging) was introduced over 30 years ago. Recent efforts prompted the development of photoremovable protecting groups that have increased photochemical efficiencies for one- and two-photon excitation to allow more sophisticated applications. This requires characterization of one- and two-photon photochemical efficiencies of the uncaging processes.The present chapter focuses on the characterization of one-photon quantum yields and two-photon cross-sections.
authors.affiliation: Department of Bioorganic Chemistry, Centre National de la Recherche Scientifique, Université Louis Pasteur Strasbourg, Illkirch, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Specht
authors.forename: Frederic
authors.initials: F
authors.lastname: Bolze
authors.forename: Jean Francois
authors.initials: JF
authors.lastname: Nicoud
authors.forename: Maurice
authors.initials: M
authors.lastname: Goeldner
mesh_terms: D001391:Azo Compounds / Q000737:chemistry / Q000528:radiation effects; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D008027:Light; D010782:Photolysis; D017785:Photons; D011789:Quantum Theory; D012015:Reference Standards; D013057:Spectrum Analysis / Q000379:methods / Q000592:standards
chemical_list: D001391:Azo Compounds; D002021:Buffers; C009850:azobenzene