title: Transplantation of differentiated bone marrow stromal cells promotes motor functional recovery in rats with stroke.
abstract: OBJECTIVE
  To investigate the effects of transplantation into rats with stroke of bone marrow-derived mesenchymal stem cells (MSCs) induced by brain-derived neurotrophic factor (BDNF).
  
  
  METHODS
  MSCs were harvested from 6-week-old Sprague-Dawley male rats, and transplanted into adult Sprague-Dawley male rats with transient middle cerebral artery occlusion. At 48 hours after stroke, the adult rats, weighing 250-280 g, were injected via the tail vein with 1×10(7) MSCs or MSCs induced by BDNF (BDNF-MSCs) suspended in 1 ml phosphate-buffered saline. All animals underwent the neurological function test for 14 days. Infarct volume and blood-brain barrier permeability assay were assessed on day 14 post-middle cerebral artery occlusion. Western blotting and immunohistochemical staining were used to detect the protein expression of neuron-specific enolase (NSE).
  
  
  RESULTS
  Both BDNF-MSCs and MSCs produced improvement in neurological deficits compared with vehicle controls on day 14 (P<0·05). In particular, the group transplanted with BDNF-MSCs exhibited significant recovery of motor function compared with the group transplanted with MSCs alone (P<0·05). Both BDNF-MSCs and MSCs, but particularly the former, reduced infarct volume, improved blood-brain barrier dysfunction, reduced serum NSE levels, activated the NSE activity, and inhibited neuronal apoptosis in the ischemic boundary zone.
  
  
  CONCLUSIONS
  BDNF-MSCs might contribute to the motor function improvement partly by reducing neuronal damage via upregulating the NSE expression level and inhibiting neuronal apoptosis.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China. hwen1229@yahoo.com.cn
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.forename: Xuean
authors.initials: X
authors.lastname: Mo
authors.forename: Chao
authors.initials: C
authors.lastname: Qin
authors.forename: Jinou
authors.initials: J
authors.lastname: Zheng
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Liang
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000502:physiology; D004195:Disease Models, Animal; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D009043:Motor Activity; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D020521:Stroke / Q000473:pathology / Q000601:surgery*
chemical_list: D010751:Phosphopyruvate Hydratase