title: Monitoring the effect of high pressure and transglutaminase treatment of milk on the evolution of flavour compounds during lactic acid fermentation using PTR-ToF-MS.
abstract: In this study, the effects of thermal or high hydrostatic pressure (HHP) treatment of a milk base in the absence or presence of a transglutaminase (TGase) protein cross-linking step on the flavour development of yoghurt were investigated. The presence of several tentatively identified volatile flavour compounds (VOCs), both during the enzymatic treatment and the lactic acid fermentation of the milk base, were monitored using a proton transfer reaction time-of-flight mass spectrometer (PTR-ToF-MS). The formation of the major flavour compounds (acetaldehyde, diacetyl, acetoin, and 2-butanone) followed a sigmoidal trend described by the modified Gompertz model. The HHP treatment of milk increased significantly the volatile compound formation rate whereas it did not affect the duration of the lag phase of formation, with the exception of acetaldehyde and diacetyl formation. On the contrary, the TGase cross-linking of milk did not significantly modify the formation rate of the volatile compounds but shortened the duration of the lag phase of their formation.
authors.affiliation: Laboratory of Food Chemistry and Technology, School of Chemical Engineering, National Technical University of Athens, Polytechnioupoli Zografou, Zografou 15780, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Tsevdou
authors.forename: Christos
authors.initials: C
authors.lastname: Soukoulis
authors.forename: Luca
authors.initials: L
authors.lastname: Cappellin
authors.forename: Flavia
authors.initials: F
authors.lastname: Gasperi
authors.forename: Petros S
authors.initials: PS
authors.lastname: Taoukis
authors.forename: Franco
authors.initials: F
authors.lastname: Biasioli
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002417:Cattle; D005285:Fermentation; D005421:Flavoring Agents / Q000032:analysis* / Q000378:metabolism; D005511:Food Handling / Q000379:methods*; D006874:Hydrostatic Pressure; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D008892:Milk / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D011503:Transglutaminases / Q000737:chemistry; D015014:Yogurt / Q000032:analysis / Q000382:microbiology
chemical_list: D005421:Flavoring Agents; D019344:Lactic Acid; D011503:Transglutaminases