title: High pressure homogenization increases antioxidant capacity and short-chain fatty acid yield of polysaccharide from seeds of Plantago asiatica L.
abstract: Physiological properties of homogenized and non-homogenized polysaccharide from the seeds of Plantago asiatica L., including antioxidant capacity and short-chain fatty acid (SCFA) production, were compared in this study. High pressure homogenization decreased particle size of the polysaccharide, and changed the surface topography from large flake-like structure to smaller porous chips. FT-IR showed that high pressure homogenization did not alter the primary structure of the polysaccharide. However, high pressure homogenization increased antioxidant capacity of the polysaccharide, evaluated by 4 antioxidant capacity assays (hydroxyl radical-scavenging, superoxide radical-scavenging, 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH)-scavenging and lipid peroxidation inhibition). Additionally, the production of total SCFA, propionic acid and n-butyric acid in ceca and colons of mice significantly increased after dieting supplementation with homogenized polysaccharide. These results showed that high pressure homogenization treatment could be a promising approach for the production of value-added polysaccharides in the food industry.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Jie-Lun
authors.initials: JL
authors.lastname: Hu
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry*; D005511:Food Handling / Q000379:methods*; D015227:Lipid Peroxidation; D051379:Mice; D010316:Particle Size; D010943:Plantago / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D011134:Polysaccharides