title: A simple method to generate adipose stem cell-derived neurons for screening purposes.
abstract: Strategies involved in mesenchymal stem cell (MSC) differentiation toward neuronal cells for screening purposes are characterized by quality and quantity issues. Differentiated cells are often scarce with respect to starting undifferentiated population, and the differentiation process is usually quite long, with high risk of contamination and low yield efficiency. Here, we describe a novel simple method to induce direct differentiation of MSCs into neuronal cells, without neurosphere formation. Differentiated cells are characterized by clear morphological changes, expression of neuronal specific markers, showing functional response to depolarizing stimuli and electrophysiological properties similar to those of developing neurons. The method described here represents a valuable tool for future strategies aimed at personalized screening of therapeutic agents in vitro.
authors.affiliation: Neuro-Zone srl, Viale Ortles 22/4, 20139, Milan, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Bossio
authors.forename: Rosa
authors.initials: R
authors.lastname: Mastrangelo
authors.forename: Raffaella
authors.initials: R
authors.lastname: Morini
authors.forename: Noemi
authors.initials: N
authors.lastname: Tonna
authors.forename: Silvia
authors.initials: S
authors.lastname: Coco
authors.forename: Claudia
authors.initials: C
authors.lastname: Verderio
authors.forename: Michela
authors.initials: M
authors.lastname: Matteoli
authors.forename: Fabio
authors.initials: F
authors.lastname: Bianco
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D061251:Primary Cell Culture / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D002118:Calcium