title: Effects of exercise level on biomarkers in a rat knee model of osteoarthritis.
abstract: The aim of this study was to elucidate whether the levels of serum biomarkers reflect the progression of osteoarthritis (OA) induced by different levels of exercise. Thirty-five Wistar rats subjected to anterior cruciate ligament transaction (ACLT) were divided into three groups: Control, moderate running, and intense running. Twelve rats (moderate running without ACLT) were allocated as a naive group. Running was performed on a motorized treadmill, at a speed of 18 m/min for 30 min/day (moderate and naive) or 60 min/day (intense) for 3 days per week. After 2 or 4 weeks, OA histopathology in the knees was evaluated using the Osteoarthritis Research Society International (OARSI) score, and the serum levels of cleaved collagen type II (C2C) and procollagen II C-propeptide (CPII) were analyzed. The OARSI score deteriorated in the intense running group after 2 weeks and the serum C2C/CPII ratio suggested the development of OA. At 4 weeks, the C2C/CPII ratio suggested there would be deterioration in the OARSI score but the score did not differ significantly between the moderate and intense running groups. C2C/CPII ratio had 13-25% correlation with the OARSI histological score. Thus, in rat experimental OA, the OARSI score could be partially predicted by the C2C/CPII ratio as a serum biomarker of OA.
authors.affiliation: Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 Shogoin, Kawahara-Cho, Sakyo-Ku, Kyoto, Japan.
authors.forename: Shoki
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Momoko
authors.initials: M
authors.lastname: Nagai
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Iijima
authors.forename: Xiangkai
authors.initials: X
authors.lastname: Zhang
authors.forename: Junichi
authors.initials: J
authors.lastname: Tajino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuroki
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D024043:Collagen Type II / Q000097:blood*; D004195:Disease Models, Animal; D018450:Disease Progression; D008297:Male; D020370:Osteoarthritis, Knee / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011347:Procollagen / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D010446:Peptide Fragments; D011347:Procollagen; C093212:procollagen type II carboxy-terminal peptide