title: The role of ADRB2 in myasthenia: genetic and immunological factors.
abstract: To examine the possibility of using ADRB2 adrenergic receptor agonists for correction of myasthenia-induced immunological and motor disturbances, a method of anti-ADRB2 antibody assay by ELISA was developed. The haplotype of ADRB2 gene was determined by MLPA-PCR in 34 myasthenia patients; blood sera from 40 patients were examined. The content of antibodies against ADRB2 receptors was elevated in 12 of 30 patients with generalized form of myasthenia. Arg-Arg in position 16 in ADRB2 receptors determines the developmental risk of side effects, while Gly-Gly in the same codon determines tolerance to β2-adrenoceptor agonists and is associated with more severe course of myasthenia and resistance to β2-adrenoceptor agonists treatment with ADRB2 agonists. The study demonstrated the possibility of using ADRB2 obtained gene engineering technique for quantitatively assay the autoantibodies.
authors.affiliation: Research Center of Neurology, Russian Academy of Medical Sciences; Bioengineering Center, Russian Academy of Sciences, Moscow.
authors.forename: V B
authors.initials: VB
authors.lastname: Lantsova
authors.forename: A S
authors.initials: AS
authors.lastname: Gerasimov
authors.forename: E K
authors.initials: EK
authors.lastname: Sepp
mesh_terms: D000293:Adolescent; D000322:Adrenergic Agonists / Q000627:therapeutic use*; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D009157:Myasthenia Gravis / Q000235:genetics / Q000276:immunology*; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics* / Q000276:immunology*; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: C553443:ADRB2 protein, human; D000322:Adrenergic Agonists; D001323:Autoantibodies; D018343:Receptors, Adrenergic, beta-2