title: Lesional accumulation of heme oxygenase-1+ microglia/macrophages in rat traumatic brain injury.
abstract: Heme oxygenase-1 (HO-1) is an inducible rate-limiting enzyme for heme degradation. Here, we studied the HO-1 expression in an open-skull weight-drop-induced traumatic brain injury, with a focus on the early phase, most amenable to therapy. In normal rat brains of our study, HO-1 cells were rarely observed. Significant parenchymal accumulation of HO-1 non-neuron cells was observed 18 h post-traumatic brain injury and increased continuously during the investigating time. We also observed that the accumulated HO-1 non-neuron cells were mainly distributed in the perilesional areas and showed activated microglia/macrophage phenotypes with ramified or amoeboid morphologic characteristics. Further double-labeling experiments showed that most HO-1 non-neuron cells coexpressed CD68 and CD163, but not glial fibrillary acid protein. Our data suggest that HO-1 expression defines a subtype of activated microglia/macrophages involved in the early processes following traumatic brain injury.
authors.affiliation: Institute of Immunology, Third Military Medical University of PLA, Chongqing, People's Republic of China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Bangwei
authors.initials: B
authors.lastname: Luo
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Schluesener
authors.forename: Zhiren
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001930:Brain Injuries / Q000201:enzymology / Q000473:pathology*; D018968:CD58 Antigens / Q000378:metabolism; D002452:Cell Count; D007839:Functional Laterality / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D007150:Immunohistochemistry; D008264:Macrophages / Q000201:enzymology / Q000473:pathology*; D017628:Microglia / Q000201:enzymology / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D011956:Receptors, Cell Surface / Q000378:metabolism; D016610:Tissue Embedding; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D018968:CD58 Antigens; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D051547:Heme Oxygenase-1