title: Cu/Zn superoxide dismutase and ascorbate peroxidase enhance in vitro shoot multiplication in transgenic plum.
abstract: In this study we examined the role of antioxidant metabolism in in vitro shoot multiplication. We generated transgenic plum plantlets overexpressing the cytsod and cytapx genes in cytosol under the control of the constitutive promoter CaMV35S. Three transgenic lines with up-regulated sod at transcriptional levels that showed silenced cytapx expression displayed an elevated in vitro multiplication rate. By contrast, a transgenic line harboring several copies of cytapx and with elevated APX enzymatic activity did not show any improvement in plant vigor, measured as the number of axillary shoots and shoot length. All of the lines with elevated micropropagation ability exhibited intensive H2O2 accumulation, monitored by 3,3'-diaminobenzidine (DAB) staining as well as by colorimetric analysis, providing direct in vitro evidence of the role of H2O2 and antioxidant genes in in vitro shoot multiplication.
authors.affiliation: Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, Faculty of Sciences, University Chouaib Doukkali, 24000 El Jadida, Morocco.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Faize
authors.forename: Lydia
authors.initials: L
authors.lastname: Faize
authors.forename: Cesar
authors.initials: C
authors.lastname: Petri
authors.forename: Gregorio
authors.initials: G
authors.lastname: Barba-Espin
authors.forename: Pedro
authors.initials: P
authors.lastname: Diaz-Vivancos
authors.forename: María José
authors.initials: MJ
authors.lastname: Clemente-Moreno
authors.forename: Tayeb
authors.initials: T
authors.lastname: Koussa
authors.forename: Lalla Aicha
authors.initials: LA
authors.lastname: Rifai
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Burgos
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Hernandez
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D007527:Isoenzymes; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D027861:Prunus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018724:Spinacia oleracea / Q000201:enzymology / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
chemical_list: D000975:Antioxidants; D007527:Isoenzymes; D010940:Plant Proteins; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase