title: Inter-laboratory study of an LC-MS/MS method for simultaneous determination of deoxynivalenol and its acetylated derivatives, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol in wheat.
abstract: To validate an LC-MS/MS method for simultaneous determination of deoxynivalenol (DON) and its acetylated derivatives, 3-acetyl-deoxynivalenol (3ADON) and 15-acetyl-deoxynivalenol (15ADON), in wheat using a multifunctional column, an inter-laboratory study was performed in 9 laboratories using one blank wheat sample, three spiked wheat samples (10, 50, 150 µg/kg) and one naturally contaminated wheat sample. The recoveries ranged from 98.8 to 102.6% for DON, 89.3 to 98.7% for 3ADON, and from 84.9 to 90.0% for 15ADON. The relative standard deviations for repeatability (RSDR) and reproducibility (RSDR) of DON were in the ranges of 7.2-11.3% and 9.5-22.6%, respectively. For 3ADON, the RSDR ranged from 5.3 to 9.5% and the RSDR ranged from 16.1 to 18.0%, while for 15ADON, the RSDR ranged from 6.2 to 11.2% and the RSDR ranged from 17.0 to 27.2%. The HorRat values for the three analytes ranged from 0.4 to 1.2. These results validate this method for the simultaneous determination of DON and its acetylated derivatives, 3ADON and 15ADON.
authors.affiliation: National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Yoshinari
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Eiichi
authors.initials: E
authors.lastname: Ishikuro
authors.forename: Masakazu
authors.initials: M
authors.lastname: Horie
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nagayama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Naito
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ohnishi
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007564:Japan; D059021:Laboratory Proficiency Testing; D015203:Reproducibility of Results; D013624:Taiwan; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*
chemical_list: D014255:Trichothecenes; C043247:3-acetyldeoxynivalenol; C046760:15-acetyldeoxynivalenol; C007262:deoxynivalenol