title: Calcitonin gene-related peptide promotes the wound healing of human bronchial epithelial cells via PKC and MAPK pathways.
abstract: Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide derived from the calcitonin gene. CGRP is widely distributed in the central and peripheral neuronal systems. In the lung, CGRP could modulate dendritic cell function, stimulate proliferation of alveolar epithelial cells and mediate lung injury in mice. In this study, we investigated the effect of CGRP on the wound healing of human bronchial epithelial cells (HBECs) in vitro. The results showed that CGRP accelerated the recovery of wound area of monolayer HBECs in a dose-dependent manner. CGRP inhibited the lipopolysaccharide-induced apoptosis in HBECs. The percentage of S phase and G2/M phase was increased in HBECs after CGRP treatment. CGRP upregulated the expression of Ki67 in a dose-dependent manner. Some pathway inhibitors were used to investigate the signal pathway in which CGRP was involved. We found out that PKC pathway inhibitor (H-7) and MAPK pathway inhibitor (PD98059) could partially attenuate the effect of CGRP, which indicated that CGRP might promote the wound healing of HBECs via PKC and/or MAPK dependent pathway by accelerating migration and proliferation, and inhibiting apoptosis.
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Sun
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Wen-Zhuo
authors.initials: WZ
authors.lastname: Ran
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.forename: Cha-Xiang
authors.initials: CX
authors.lastname: Guan
mesh_terms: D017209:Apoptosis; D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D011493:Protein Kinase C / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects; D014945:Wound Healing
chemical_list: D008070:Lipopolysaccharides; D011493:Protein Kinase C; D015740:Calcitonin Gene-Related Peptide