title: A UPLC-MS/MS method for qualification of quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside in rat plasma and application to pharmacokinetic studies.
abstract: A sensitive and accurate ultra-performance liquid chromatography coupled with triple quadrupole mass (UPLC-MS/MS) method was developed for the determination of quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside (QGR) in rat plasma using rutin as internal standard. Chromatographic separation was achieved on a Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with a gradient elution of acetonitrile and 0.10% formic acid (v/v) at a flow rate of 0.4 mL/min. QGR and rutin were detected using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. The method demonstrated good linearity and did not show any endogenous interference with the QGR and rutin peaks. This method was successfully applied to a pharmacokinetic study of QGR in rats after intravenous (20 mg/kg) and oral (40 mg/kg) administration, and the results showed that the compound was poorly absorbed, with an absolute bioavailability of approximately 3.41%.
authors.affiliation: Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.forename: Guisheng
authors.initials: G
authors.lastname: Zhou
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Zhenhao
authors.initials: Z
authors.lastname: Li
authors.forename: Shulan
authors.initials: S
authors.lastname: Su
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000097:blood* / Q000737:chemistry* / Q000493:pharmacokinetics; D007700:Kinetics; D008297:Male; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D005960:Glucosides; D011794:Quercetin; C110309:quercetin 3-O-glucopyranoside