title: Design and optimization of reaction chamber and detection system in dynamic labs-on-chip for proteins detection.
abstract: In this paper, the lab-on-chip section for a protein assay is designed and optimized. To avoid severe reliability problems related to activated surface stability, a dynamic assay approach is adopted: protein-to-protein neutralization is performed while proteins diffuse freely in the reaction chamber. The related refraction index change is detected via an integrated interferometer. The structure is also design to provide a functional test of the reference protein solution, which is generally required for qualification for medical uses.
authors.affiliation: Istituto per Applicazioni del Calcolo M. Picone, CNR, Rome I-00185, Italy. m.briani@iac.cnr.it
authors.forename: Maya
authors.initials: M
authors.lastname: Briani
authors.forename: Giacomo
authors.initials: G
authors.lastname: Germani
authors.forename: Eugenio
authors.initials: E
authors.lastname: Iannone
authors.forename: Maurizio
authors.initials: M
authors.lastname: Moroni
authors.forename: Roberto
authors.initials: R
authors.lastname: Natalini
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D007368:Interferometry / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011506:Proteins