title: Infrared-based protein detection arrays for quantitative proteomics.
abstract: BACKGROUND
  Protein microarrays have the potential to join the field of quantitative proteomics as a standard method. This antibody-based experimental platform allows the highly sensitive and highly specific quantification of selected target proteins and excels with high sample capacity. As a key feature, numerous arrays can be produced in parallel, thus minimizing sample consumption.
  
  
  OBJECTIVE
  The recent progress made in the field of reverse phase protein arrays is summarized, with a focus on the introduction of normalization measures, as introduced in the authors' infrared-based protein arrays with quantitative readout approach.
  
  
  CONCLUSIONS
  Tumor biology as well as drug discovery applications will soon benefit from the comprehensive description of signaling pathways and protein microarrays are an appropriate tool to achieve this goal.
authors.affiliation: German Cancer Research Center (DKFZ), Department of Molecular Genome Analysis/B050, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany +49 6221 424765 ; +49 6221 423454 ; u.korf@dkfz.de.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Korf
authors.forename: Christian
authors.initials: C
authors.lastname: Löbke
authors.forename: Florian
authors.initials: F
authors.lastname: Haller
authors.forename: Holger
authors.initials: H
authors.lastname: Sültmann
authors.forename: Annemarie
authors.initials: A
authors.lastname: Poustka