title: Recurrent laryngeal nerve regeneration through a silicone tube produces reinnervation without vocal fold mobility in rats.
abstract: OBJECTIVE
  We established an animal model of recurrent laryngeal nerve reinnervation with persistent vocal fold immobility following recurrent laryngeal nerve injury.
  
  
  METHODS
  In 36 rats, the left recurrent laryngeal nerve was transected and the stumps were abutted in a silicone tube with a 1-mm interspace, facilitating regeneration. The mobility of the vocal folds was examined endoscopically 5, 10, and 15 weeks later. Electromyography of the thyroarytenoid muscle was performed. Reinnervation was assessed by means of a quantitative immunohistologic evaluation with anti-neurofilament antibody in the nerve both proximal and distal to the silicone tube. The atrophy of the thyroarytenoid muscle was assessed histologically.
  
  
  RESULTS
  We observed that all animals had a fixed left vocal fold throughout the study. The average neurofilament expression in the nerve both distal and proximal to the silicone tube, the muscle area, and the amplitude of the compound muscle action potential recorded from the thyroarytenoid muscle on the treated side increased significantly (p < 0.05) over time, demonstrating regeneration through the silicone tube.
  
  
  CONCLUSIONS
  Recurrent laryngeal nerve regeneration through a silicone tube produced reinnervation without vocal fold mobility in rats. The efficacy of new laryngeal reinnervation treatments can be assessed with this model.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University Graduate School of Medicine, Kumamoto, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kumai
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoyama
authors.forename: Kohei
authors.initials: K
authors.lastname: Nishimoto
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Sanuki
authors.forename: Ryosei
authors.initials: R
authors.lastname: Minoda
authors.forename: Eiji
authors.initials: E
authors.lastname: Yumoto
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004576:Electromyography; D004867:Equipment Design; D005260:Female; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D007821:Laryngeal Muscles / Q000294:innervation; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012009:Recurrent Laryngeal Nerve / Q000502:physiology*; D061226:Recurrent Laryngeal Nerve Injuries / Q000150:complications / Q000503:physiopathology / Q000628:therapy*; D012828:Silicones; D014826:Vocal Cord Paralysis / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D014827:Vocal Cords / Q000294:innervation*
chemical_list: D012828:Silicones