title: Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus.
abstract: We show that a previously annotated hypothetical protein is the transposase of a new and active IS element, ISCahy1, widespread in Caldicellulosiruptor species. Transposition generated an 11-bp direct repeat at the insertion site in Caldicellulosiruptor hydrothermalis, suggesting a cut-and-paste mechanism. The discovery of an active insertion sequence in Caldicellulosiruptor species led to a survey of potential IS elements in the genome sequences of eight Caldicellulosiruptor species that identified several new elements, including one novel to this genus.
authors.affiliation: Department of Genetics, University of Georgia, Athens, GA 30602, USA.
authors.forename: Daehwan
authors.initials: D
authors.lastname: Chung
authors.forename: Joel
authors.initials: J
authors.lastname: Farkas
authors.forename: Janet
authors.initials: J
authors.lastname: Westpheling
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D019895:Transposases / Q000235:genetics / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases