title: A culture model to analyze the acute biomaterial-dependent reaction of human primary macrophages.
abstract: Macrophages are important in foreign body reactions. We devised a culture model with human primary macrophages to evaluate the acute response of macrophages to biomaterials. First we selected proteins representative for pro-inflammatory (M1) or anti-inflammatory/repair (M2) response of monocytes isolated from blood of healthy human donors by exposing them to LPS+IFNγ or IL-4. A relative M1/M2 index was calculated using IL-1β, IL-6, tumor necrosis factor (TNF)α, monocyte chemotactic protein (MCP)-3 and macrophage inflammatory protein (MIP)-1α as M1 markers, and IL-1 receptor antagonist (IL-1RA), CCL18, regulated and normal T-cell expressed and secreted (RANTES), and macrophage-derived chemokine (MDC) as M2 markers. Then monocytes were cultured for 3days on 4 materials selected for known different foreign body reactions: Permacol™, Parietex™ Composite, multifilament polyethylene terephthalate and multifilament polypropylene. Macrophages on polypropylene produced high levels of anti-inflammatory proteins with a low M1/M2 index. Macrophages on Parietex™ Composite produced high levels of inflammatory and anti-inflammatory proteins, with a high M1/M2 index. Macrophages on polyethylene terephthalate also resulted in a high M1/M2 index. Macrophages on Permacol™ produced a low amount of all proteins, with a low M1/M2 index. This model with human primary macrophages and the panel of read-out parameters can be used to evaluate the acute reaction of macrophages to biomaterials in vitro to get more insight in the foreign body reaction.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus MC, University Medical Centre Rotterdam, Ee1618, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands. n.grotenhuis@erasmusmc.nl
authors.forename: N
authors.initials: N
authors.lastname: Grotenhuis
authors.forename: Y
authors.initials: Y
authors.lastname: Bayon
authors.forename: J F
authors.initials: JF
authors.lastname: Lange
authors.forename: G J V M
authors.initials: GJ
authors.lastname: Van Osch
authors.forename: Y M
authors.initials: YM
authors.lastname: Bastiaansen-Jenniskens
mesh_terms: D001672:Biocompatible Materials / Q000009:adverse effects*; D002478:Cells, Cultured; D054405:Chemokine CCL3 / Q000096:biosynthesis; D003094:Collagen / Q000009:adverse effects; D016207:Cytokines / Q000096:biosynthesis; D005549:Foreign-Body Reaction / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology*; D015870:Gene Expression; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008422:Materials Testing; D008954:Models, Biological; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D011093:Polyethylene Terephthalates / Q000009:adverse effects; D011126:Polypropylenes / Q000009:adverse effects
chemical_list: D001672:Biocompatible Materials; C515429:CCL3 protein, human; D054405:Chemokine CCL3; D016207:Cytokines; D018836:Inflammation Mediators; C422414:Permacol; D011093:Polyethylene Terephthalates; D011126:Polypropylenes; D003094:Collagen