title: High-resolution melting analysis for the detection of two erythromycin-resistant Bordetella pertussis strains carried by healthy schoolchildren in China.
abstract: Two erythromycin-resistant strains of Bordetella pertussis were isolated from nasopharyngeal specimens of two asymptomatic schoolchildren in China. High-resolution melting and sequencing analyses confirmed the homogeneous A2047G mutation in 23S rRNA genes of the two isolates. High-resolution melting (HRM) analysis is a useful assay for the rapid detection of erythromycin-resistant B. pertussis. The appearance of erythromycin-resistant B. pertussis strains in China is alarming.
authors.affiliation: National Centre for Chronic and Non-communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China. zhangqi@chinacdc.cn
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Xin
authors.forename: Q
authors.initials: Q
authors.lastname: He
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001886:Bordetella pertussis / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D002648:Child; D002681:China / Q000453:epidemiology; D003430:Cross-Sectional Studies; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D008297:Male; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D012338:RNA, Ribosomal, 23S; D016036:Seroepidemiologic Studies; D014917:Whooping Cough / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D004917:Erythromycin