title: Karyotype analysis in large-sample infertile couples living in Central China: a study of 14965 couples.
abstract: OBJECTIVE
  To explore that it is necessary to routinely detect chromosomes in fertile couples, we detected peripheral blood lymphocyte karyotype in 14965 infertile couples living in Central China and analyzed the incidence and type of chromosomal anomaly.
  
  
  METHODS
  G-banding karyotype analysis of peripheral blood lymphocytes was performed in 14965 couples who went to the outpatient department of our reproductive medical center for counseling on infertility between January 2004 and December 2011. Semen analysis was performed three times in all the men from the 14965 couples.
  
  
  RESULTS
  The rate of chromosomal anomaly in the 14965 infertile couples was 3.84 % (1150/29930). The rate of chromosomal anomaly in the men from 14965 couples was 6.84 % (1024/14965) and in the women 0.84 % (126/14965). The rates of chromosomal anomaly were 1.69 % in normal semen group, 11.82 % in light oligo-astheno-spermis group, 6.58 % in moderate to severe olig-astheno-spermia group and 17.26 % in azoospermia group.
  
  
  CONCLUSIONS
  Since the rates of chromosomal anomaly are 1.69 % and 11.82 % even in normal semen group and light oligo-astheno-spermia group, respectively, it is necessary to detect peripheral blood lymphocyte karyotype in all infertile couples.
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University, Number one, Constructive East Road, Zhengzhou City, 450052, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Kong
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Wu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.forename: Ying-Pu
authors.initials: YP
authors.lastname: Sun
mesh_terms: D000328:Adult; D002681:China; D002869:Chromosome Aberrations; D005191:Family Characteristics; D005260:Female; D006801:Humans; D007246:Infertility / Q000208:ethnology / Q000235:genetics*; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics*; D059785:Karyotype; D008297:Male; D012661:Semen / Q000166:cytology; D055101:Semen Analysis; D013094:Spermatozoa / Q000378:metabolism