title: Cooperation between osteoblastic cells and endothelial cells enhances their phenotypic responses and improves osteoblast function.
abstract: Osteogenesis requires close co-operation with angiogenesis to create vascularized bone tissue. In this study, an indirect co-culture model using osteoblasts (OBs), primary endothelial cells (ECs) and Matrigel interlayer was established to understand the impact of each cell type on the other. ECs synergistically enhanced osteoblastic gene expression by OBs, while OBs were capable of supporting tubule-like structures formed by ECs on Matrigel, enhancing mean tubule length from 146.5 ± 23.5 μm in ECs alone to 192 ± 28.6 μm in co-culture (p < 0.05). Similar improvements were noted in terms of tubule number. An applicability study of the co-culture model to bone tissue engineering, performed on a biopolymer fibrous membrane, showed substantially enhanced deposition of calcified nodules. These results demonstrate the efficacy of co-culture with ECs to improve osteogenesis for bone tissue engineering.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea.
authors.forename: Tuvd
authors.initials: T
authors.lastname: Dariima
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Jin
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Lee
authors.forename: Ivan B
authors.initials: IB
authors.lastname: Wall
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D001846:Bone Development; D018920:Coculture Techniques / Q000379:methods; D003094:Collagen; D004338:Drug Combinations; D042783:Endothelial Cells / Q000502:physiology*; D007797:Laminin; D010006:Osteoblasts / Q000502:physiology*; D011509:Proteoglycans; D023822:Tissue Engineering / Q000379:methods
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen