title: Centrifugal partition extraction of β-carotene from Dunaliella salina for efficient and biocompatible recovery of metabolites.
abstract: A biocompatible extraction method for β-carotene recovery from the microalga Dunaliella salina was studied. The centrifugal partition extraction was used for liquid-liquid mass transfer intensification during continuous extraction. Different solvents and process parameters were compared. Ethyl oleate extraction with 5% dichloromethane achieved a 65% β-carotene recovery with the least amount of cell damage as more than 65% of the cells remained viable as demonstrated by photosynthesis activity measurements.
authors.affiliation: LUNAM, GEPEA Laboratory UMR CNRS 6144, 37 bd de l'Université, 44600 Saint-Nazaire, France. luc.marchal@univ-nantes.fr
authors.forename: Luc
authors.initials: L
authors.lastname: Marchal
authors.forename: Mariem
authors.initials: M
authors.lastname: Mojaat-Guemir
authors.forename: Alain
authors.initials: A
authors.lastname: Foucault
authors.forename: Jérémy
authors.initials: J
authors.lastname: Pruvost
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002498:Centrifugation; D000460:Chlorophyta / Q000378:metabolism*; D055442:Metabolome; D058086:Microalgae / Q000378:metabolism; D050296:Microbial Viability; D010788:Photosynthesis; D012212:Rheology; D012399:Rotation; D012997:Solvents; D019207:beta Carotene / Q000302:isolation & purification*
chemical_list: D012997:Solvents; D019207:beta Carotene