title: The Influence of Competition AmongC. elegansSmall RNA Pathways on Development.
abstract: Small RNAs play a variety of regulatory roles, including highly conserved developmental functions.Caenorhabditis elegansnot only possesses most known small RNA pathways, it is also an easy system to study their roles and interactions during development. It has been proposed that inC. elegans, some small RNA pathways compete for access to common limiting resources. The strongest evidence supporting this model is that disrupting the production or stability of endogenous short interfering RNAs (endo-siRNAs) enhances sensitivity to experimentally induced exogenous RNA interference (exo-RNAi). Here, we examine the relationship between the endo-siRNA and microRNA (miRNA) pathways, and find that, consistent with competition among these endogenous small RNA pathways, endo-siRNA pathway mutants may enhance miRNA efficacy. Furthermore, we show that exo-RNAi may also compete with both endo-siRNAs and miRNAs. Our data thus provide support that all known Dicer-dependent small RNA pathways may compete for limiting common resources. Finally, we observed that both endo-siRNA mutants and animals experiencing exo-RNAi have increased expression of miRNA-regulated stage-specific developmental genes. These observations suggest that perturbing the small RNA flux and/or the induction of exo-RNAi, even in wild-type animals, may impact development via effects on the endo-RNAi and microRNA pathways.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Biological Laboratories, Room 3044, 16 Divinity Avenue, Cambridge, MA 02138, USA.
authors.forename: Jimmy J
authors.initials: JJ
authors.lastname: Zhuang
authors.forename: Craig P
authors.initials: CP
authors.lastname: Hunter
keywords: RNA regulation; endogenous siRNAs; enhanced RNAi; exogenous RNAi; microRNAs