title: Fragment screening by biochemical assay.
abstract: The use of high concentration biochemical assays to identify weak binding fragment molecules can be an effective method to identify novel starting points for medicinal chemistry programmes. The combination of a high-quality fragment library with sensitive biochemical screening methods is a viable alternative to the more commonly used fragment screening methods such as nuclear magnetic resonance screening or high-throughput X-ray crystallography. Notably, there are a number of literature reports where fragment molecules have been identified by a high concentration biochemical assay. The use of high concentration screening of fragments using a portfolio of single-molecule fluorescence correlation spectroscopy detection techniques to ensure the highest reproducibility and sensitivity have been demonstrated, as well as the use of and X-ray crystallography to determine the binding mode of active fragments.
authors.affiliation: Evotec, 111 Milton Park, Abingdon, Oxon, OX14 4RZ, UK. mark.whittaker@evotec.com.
authors.forename: John
authors.initials: J
authors.lastname: Barker
authors.forename: Steve
authors.initials: S
authors.lastname: Courtney
authors.forename: Thomas
authors.initials: T
authors.lastname: Hesterkamp
authors.forename: Dirk
authors.initials: D
authors.lastname: Ullmann
authors.forename: Mark
authors.initials: M
authors.lastname: Whittaker