title: Label-free biosensing with single-molecule force spectroscopy.
abstract: We demonstrate here a novel single-molecule, label-free bioanalytical system capable of sensing the presence of specific ssDNA oligomer sequences and proteins with high selectivity and sensitivity. An ssDNA concentration of 1 nM and a Lyz concentration of 0.65 nM could be detected.
authors.affiliation: Hybrid Materials Interfaces Group, Faculty of Production Engineering, University of Bremen, D-28359 Bremen, Germany. wei@uni-bremen.de
authors.forename: Gang
authors.initials: G
authors.lastname: Wei
authors.forename: Sascha
authors.initials: S
authors.lastname: Steckbeck
authors.forename: Susan
authors.initials: S
authors.lastname: Köppen
authors.forename: Lucio
authors.initials: L
authors.lastname: Colombi Ciacchi
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000032:analysis*; D006108:Graphite / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes; D004277:DNA, Single-Stranded; D012822:Silicon Dioxide; D006108:Graphite; D009113:Muramidase