title: Smoking decreases the response of human lung macrophages to double-stranded RNA by reducing TLR3 expression.
abstract: BACKGROUND
  Cigarette smoking is associated with increased frequency and duration of viral respiratory infections, but the underlying mechanisms are incompletely defined. We investigated whether smoking reduces expression by human lung macrophages (Mø) of receptors for viral nucleic acids and, if so, the effect on CXCL10 production.
  
  
  METHODS
  We collected alveolar macrophages (AMø) by bronchoalveolar lavage of radiographically-normal lungs of subjects undergoing bronchoscopies for solitary nodules (n = 16) and of volunteers who were current or former smokers (n = 7) or never-smokers (n = 13). We measured expression of mRNA transcripts for viral nucleic acid receptors by real-time PCR in those AMø and in the human Mø cell line THP-1 following phorbol myristate acetate/vitamin D3 differentiation and exposure to cigarette smoke extract, and determined TLR3 protein expression using flow cytometry and immunohistochemistry. We also used flow cytometry to examine TLR3 expression in total lung Mø from subjects undergoing clinically-indicated lung resections (n = 25). Of these, seven had normal FEV1 and FEV1/FVC ratio (three former smokers, four current smokers); the remaining 18 subjects (14 former smokers; four current smokers) had COPD of GOLD stages I-IV. We measured AMø production of CXCL10 in response to stimulation with the dsRNA analogue poly(I:C) using Luminex assay.
  
  
  RESULTS
  Relative to AMø of never-smokers, AMø of smokers demonstrated reduced protein expression of TLR3 and decreased mRNA for TLR3 but not TLR7, TLR8, TLR9, RIG-I, MDA-5 or PKR. Identical changes in TLR3 gene expression were induced in differentiated THP-1 cells exposed to cigarette smoke-extract in vitro for 4 hours. Among total lung Mø, the percentage of TLR3-positive cells correlated inversely with active smoking but not with COPD diagnosis, FEV1% predicted, sex, age or pack-years. Compared to AMø of never-smokers, poly(I:C)-stimulated production of CXCL10 was significantly reduced in AMø of smokers.
  
  
  CONCLUSIONS
  Active smoking, independent of COPD stage or smoking duration, reduces both the percent of human lung Mø expressing TLR3, and dsRNA-induced CXCL10 production, without altering other endosomal or cytoplasmic receptors for microbial nucleic acids. This effect provides one possible mechanism for increased frequency and duration of viral lower respiratory tract infections in smokers.
  
  
  BACKGROUND
  ClinicalTrials.gov NCT00281190, NCT00281203 and NCT00281229.
authors.affiliation: Division of Pulmonary & Critical Care Medicine, Department of Internal Medicine, University of Michigan Health Care System, Ann Arbor, MI, 48109-2399, USA.
authors.forename: Jill C
authors.initials: JC
authors.lastname: Todt
authors.forename: Christine M
authors.initials: CM
authors.lastname: Freeman
authors.forename: Jeanette P
authors.initials: JP
authors.lastname: Brown
authors.forename: Joanne
authors.initials: J
authors.lastname: Sonstein
authors.forename: Theresa M
authors.initials: TM
authors.lastname: Ames
authors.forename: Alexandra L
authors.initials: AL
authors.lastname: McCubbrey
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Martinez
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Chensue
authors.forename: James M
authors.initials: JM
authors.lastname: Beck
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Curtis
mesh_terms: D000328:Adult; D000368:Aged; D002460:Cell Line; D002478:Cells, Cultured; D015331:Cohort Studies; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism / Q000821:virology; D016676:Macrophages, Alveolar / Q000378:metabolism* / Q000821:virology; D008297:Male; D008875:Middle Aged; D012330:RNA, Double-Stranded / Q000037:antagonists & inhibitors* / Q000235:genetics; D012907:Smoking / Q000235:genetics / Q000378:metabolism*; D051196:Toll-Like Receptor 3 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D055815:Young Adult
chemical_list: D012330:RNA, Double-Stranded; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3