title: Fabrication, characterization and application of pectin degrading Fe3O4-SiO2 nanobiocatalyst.
abstract: The covalent binding of pectinase onto amino functionalized silica-coated magnetic nanoparticles (CSMNPs) through glutaraldehyde activation was investigated for nanobiocatalyst fabrication. The average particle size and morphology of the nanoparticles were characterized using transmission electron microscopy (TEM). The statistical analysis for TEM image suggests that the coating and binding process did not cause any significant change in size of MNPs. The morphological and phase change of the magnetic nanoparticles (MNPs) after various coatings and immobilization were characterized by X-ray diffraction (XRD) studies. The various surface modifications and pectinase binding onto nanoparticles were confirmed by Fourier transform infrared (FT-IR) spectroscopy. The maximum activity of immobilized pectinase was obtained at its weight ratio of 19.0×10(-3) mg bound pectinase/mg CSMNPs. The pH, temperature, reusability, storage ability and kinetic studies were established to monitor their improved stability and activity of the fabricated nanobiocatalyst. Furthermore, the application was extended in the clarification of Malus domestica juice.
authors.affiliation: Department of Biotechnology, Madha Engineering College, Chennai, India.
authors.forename: Muthulingam
authors.initials: M
authors.lastname: Seenuvasan
authors.forename: Carlin Geor
authors.initials: CG
authors.lastname: Malar
authors.forename: Sridhar
authors.initials: S
authors.lastname: Preethi
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Balaji
authors.forename: Jeyaraj
authors.initials: J
authors.lastname: Iyyappan
authors.forename: Madhava Anil
authors.initials: MA
authors.lastname: Kumar
authors.forename: Kannaiyan Sathish
authors.initials: KS
authors.lastname: Kumar
mesh_terms: D001628:Beverages; D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000648:ultrastructure; D027845:Malus / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D010368:Pectins / Q000378:metabolism*; D011096:Polygalacturonase / Q000378:metabolism*; D059027:Recycling; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D010368:Pectins; D011096:Polygalacturonase; D052203:Ferrosoferric Oxide