title: Enzyme catalysed tandem reactions.
abstract: To transfer to the laboratory, the excellent efficiency shown by enzymes in Nature, biocatalysis, had to mimic several synthetic strategies used by the living organisms. Biosynthetic pathways are examples of tandem catalysis and may be assimilated in the biocatalysis field for the use of isolated multi-enzyme systems in the homogeneous phase. The concurrent action of several enzymes that work sequentially presents extraordinary advantages from the synthetic point of view, since it permits a reversible process to become irreversible, to shift the equilibrium reaction in such a way that enantiopure compounds can be obtained from prochiral or racemic substrates, reduce or eliminate problems due to product inhibition or prevent the shortage of substrates by dilution or degradation in the bulk media, etc. In this review we want to illustrate the developments of recent studies involving in vitro multi-enzyme reactions for the synthesis of different classes of organic compounds.
authors.affiliation: Departamento de Química Bio-Orgánica, Instituto de Química Orgánica General, CSIC, Juan de Cierva 3, 28006 Madrid, Spain. isabel.oroz@iqog.csic.es
authors.forename: Isabel
authors.initials: I
authors.lastname: Oroz-Guinea
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-Junceda
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D001709:Biotechnology; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D010940:Plant Proteins