title: The pEAQ vector series: the easy and quick way to produce recombinant proteins in plants.
abstract: The pEAQ vectors are a series of plasmids designed to allow easy and quick production of recombinant proteins in plants. Their main feature is the use of the Cowpea Mosaic Virus hypertranslational "CPMV-HT" expression system, which provides high yields of recombinant protein through extremely high translational efficiency without the need for viral replication. Since their creation, the pEAQ vectors have been used to produce a wide variety of proteins in plants. Viral proteins and Virus-Like Particles (VLPs) have been of particular interest, but other types of proteins including active enzymes have also been expressed. While the pEAQ vectors have mostly been used in a transient expression context, through agroinfiltration of leaves, they have also been shown to be suitable for the production of stably transformed lines of both cell cultures and whole plants. This paper looks back on the genesis of the pEAQ vectors and reviews their use so far.
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK. hadrien.peyret@jic.ac.uk
authors.forename: Hadrien
authors.initials: H
authors.lastname: Peyret
authors.forename: George P
authors.initials: GP
authors.lastname: Lomonossoff
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D017798:Comovirus / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D015203:Reproducibility of Results; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014612:Vaccines / Q000096:biosynthesis / Q000235:genetics
chemical_list: D008049:Lipase; D011994:Recombinant Proteins; D014612:Vaccines; C485982:LIPF protein, human