title: DNA shuffling of cytochromes P450 for indigoid pigment production.
abstract: DNA family shuffling is a powerful method of directed evolution applied for the generation of novel enzymes with the aim of improving their existing features or creating completely new enzyme properties. This method of evolution in vitro requires parental sequences containing a high level of sequence similarity, such as is found in family members of cytochrome P450 enzymes. Cytochromes P450 (P450s or CYPs) are capable of catalyzing a variety of chemical reactions and generating a wide range of products including dye production (e.g., pigments indigo and indirubin). Application of the method of DNA family shuffling described here has enabled us to create novel P450 enzymes and to further extend the capacity of P450 to oxidize indole, leading to pigment formation.
authors.affiliation: School of Biological Sciences, University of Queensland, St. Lucia, QLD, Australia.
authors.forename: Nedeljka N
authors.initials: NN
authors.lastname: Rosic
mesh_terms: D003001:Cloning, Molecular; D004396:Coloring Agents / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D032621:DNA Shuffling / Q000379:methods*; D015723:Gene Library; D006801:Humans; D007203:Indigo Carmine; D007211:Indoles / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques
chemical_list: D004396:Coloring Agents; D007211:Indoles; D003577:Cytochrome P-450 Enzyme System; D007203:Indigo Carmine; C027185:indirubin