title: Dehydroepiandrosterone protects endothelial cells against inflammatory events induced by urban particulate matter and titanium dioxide nanoparticles.
abstract: Particulate matter (PM) and nanoparticles (NPs) induce activation and dysfunction of endothelial cells characterized by inhibition of proliferation, increase of adhesion and adhesion molecules expression, increase of ROS production, and death. DHEA has shown anti-inflammatory and antioxidant properties in HUVEC activated with proinflammatory agents. We evaluated if DHEA could protect against some inflammatory events produced by PM10 and TiO2 NPs in HUVEC. Adhesion was evaluated by a coculture with U937 cells, proliferation by crystal violet staining, and oxidative stress through DCFDA and Griess reagent. PM10 and TiO2 NPs induced adhesion and oxidative stress and inhibited proliferation of HUVEC; however, when particles were added in combination with DHEA, the effects previously observed were abolished independently from the tested concentrations and the time of addition of DHEA to the cultures. These results indicate that DHEA exerts significant anti-inflammatory and antioxidative effects on the damage induced by particles in HUVEC, suggesting that DHEA could be useful to counteract the harmful effects and inflammatory diseases induced by PM and NPs.
authors.affiliation: Departamento de Biología Celular, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1, Colonia Sección 16, Tlalpan, 14080 México, DF, Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Huerta-García
authors.forename: Angélica
authors.initials: A
authors.lastname: Montiél-Dávalos
authors.forename: Ernesto
authors.initials: E
authors.lastname: Alfaro-Moreno
authors.forename: Gisela
authors.initials: G
authors.lastname: Gutiérrez-Iglesias
authors.forename: Rebeca
authors.initials: R
authors.lastname: López-Marure
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D053758:Nanoparticles / Q000009:adverse effects*; D052638:Particulate Matter / Q000009:adverse effects* / Q000494:pharmacology; D014025:Titanium / Q000009:adverse effects* / Q000494:pharmacology; D020298:U937 Cells
chemical_list: D000276:Adjuvants, Immunologic; D052638:Particulate Matter; C009495:titanium dioxide; D003687:Dehydroepiandrosterone; D014025:Titanium