title: Stable-isotope dilution LC-MS/MS assay for determination of iloperidone and its two major metabolites, P 88 and P 95, in human plasma: application to a bioequivalence study.
abstract: BACKGROUND
  Iloperidone is a novel antipsychotic drug with high affinity for serotonin and D(2) dopamine receptors. A sensitive and rapid isotope dilution LC-MS/MS method has been developed for the simultaneous determination of iloperidone and its two major metabolites in human plasma.
  
  
  RESULTS
  The analytes and their deuterated analogs as internal standards were quantitatively extracted from 100-µl human plasma by SPE. The method was validated over the concentration range of 0.01-6 ng/ml for all the analytes. Baseline separation of analytes was possible within 3 min on ACE 5 C8 column. The accuracy and precision (%CV) of the method varied from 96.2 to 105%, and 1.17 to 4.75%, respectively. The extraction recovery was >84%, while the internal standard-normalized matrix factors ranged from 0.97-1.03 for all three analytes.
  
  
  CONCLUSIONS
  The developed method was successfully applied to support a bioequivalence study in healthy volunteers.
authors.affiliation: Chemistry Department, Kadi Sarva Vishwavidyalaya, Sarva Vidyalaya Campus, Gujarat, India.
authors.forename: Jignesh M
authors.initials: JM
authors.lastname: Parekh
authors.forename: Mallika
authors.initials: M
authors.lastname: Sanyal
authors.forename: Manish
authors.initials: M
authors.lastname: Yadav
authors.forename: Pranav S
authors.initials: PS
authors.lastname: Shrivastav
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D003903:Deuterium / Q000737:chemistry; D006207:Half-Life; D006801:Humans; D007553:Isotope Labeling; D007555:Isoxazoles / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D010880:Piperidines / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000592:standards; D013810:Therapeutic Equivalency
chemical_list: D014150:Antipsychotic Agents; D007555:Isoxazoles; D010880:Piperidines; D003903:Deuterium; C081732:iloperidone