title: A plasmodesmal glycosyltransferase-like protein.
abstract: Plasmodesmata (Pd) are plant intercellular connections that represent cytoplasmic conduits for a wide spectrum of cellular transport cargoes, from ions to house-keeping proteins to transcription factors and RNA silencing signals; furthermore, Pd are also utilized by most plant viruses for their spread between host cells. Despite this central role of Pd in the plant life cycle, their structural and functional composition remains poorly characterized. In this study, we used a known Pd-associated calreticulin protein AtCRT1 as bait to isolate other Pd associated proteins in Arabidopsis thaliana. These experiments identified a beta-1,6-N-acetylglucosaminyl transferase-like enzyme (AtGnTL). Subcellular localization studies using confocal microscopy observed AtGnTL at Pd within living plant cells and demonstrated colocalization with a Pd callose-binding protein (AtPDCB1). That AtGnTL is resident in Pd was consistent with its localization within the plant cell wall following plasmolysis. Initial characterization of an Arabidopsis T-DNA insertional mutant in the AtGnTL gene revealed defects in seed germination and delayed plant growth.
authors.affiliation: Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York, United States of America.
authors.forename: Lisa
authors.initials: L
authors.lastname: Zalepa-King
authors.forename: Vitaly
authors.initials: V
authors.lastname: Citovsky
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000166:cytology / Q000201:enzymology; D031425:Plasmodesmata / Q000201:enzymology*; D021381:Protein Transport
chemical_list: D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase