title: IL-6 production by dendritic cells is dispensable for CD8+ memory T-cell generation.
abstract: Following activation, naïve CD8(+) T cells will differentiate into effectors that differ in their ability to survive: some will persist as memory cells while the majority will die by apoptosis. Signals given by antigen-presenting cells (APCs) at the time of priming modulate this differential outcome. We have recently shown that, in opposition to dendritic cell (DC), CD40-activated B-(CD40-B) cell vaccination fails to efficiently produce CD8(+) memory T cells. Understanding why CD40-B-cell vaccination does not lead to the generation of functional long-lived memory cells is essential to define the signals that should be provided to naïve T cells by APCs. Here we show that CD40-B cells produce very low amount of IL-6 when compared to DCs. However, supplementation with IL-6 during CD40-B-cell vaccination did not improve memory generation. Furthermore, IL-6-deficient DCs maintained the capacity to promote the formation of functional CD8(+) effectors and memory cells. Our results suggest that in APC vaccination models, IL-6 provided by the APCs is dispensable for proper CD8(+) T-cell memory generation.
authors.affiliation: Maisonneuve-Rosemont Hospital Research Center, University of Montreal, 5415 Boulevard de l'Assomption, Montréal, QC, Canada H1T 2M4.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Daudelin
authors.forename: Mélissa
authors.initials: M
authors.lastname: Mathieu
authors.forename: Salix
authors.initials: S
authors.lastname: Boulet
authors.forename: Nathalie
authors.initials: N
authors.lastname: Labrecque
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation; D019013:CD40 Antigens / Q000235:genetics / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D007156:Immunologic Memory; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout
chemical_list: D019013:CD40 Antigens; D015850:Interleukin-6