title: Imaging fully hydrated whole cells by coherent x-ray diffraction microscopy.
abstract: Nanoscale imaging of biological specimens in their native condition is of long-standing interest, in particular with direct, high resolution views of internal structures of intact specimens, though as yet progress has been limited. Here we introduce wet coherent x-ray diffraction microscopy capable of imaging fully hydrated and unstained biological specimens. Whole cell morphologies and internal structures better than 25 nm can be clearly visualized without contrast degradation.
authors.affiliation: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
authors.forename: Daewoong
authors.initials: D
authors.lastname: Nam
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Park
authors.forename: Marcus
authors.initials: M
authors.lastname: Gallagher-Jones
authors.forename: Sangsoo
authors.initials: S
authors.lastname: Kim
authors.forename: Sunam
authors.initials: S
authors.lastname: Kim
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kohmura
authors.forename: Hisashi
authors.initials: H
authors.lastname: Naitow
authors.forename: Naoki
authors.initials: N
authors.lastname: Kunishima
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Changyong
authors.initials: C
authors.lastname: Song
mesh_terms: D000458:Cyanobacteria / Q000166:cytology / Q000378:metabolism; D046931:Microcystis / Q000166:cytology / Q000378:metabolism; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D014867:Water