title: Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk.
abstract: A novel rapid (20 min) fluorescent lateral flow test for chloramphenicol (CAP) detection in milk was developed. The chosen format is a binding-inhibition assay. Water-soluble quantum dots with an emission peak at 625 nm were applied as a label. Milk samples were diluted by 20 % with phosphate buffer to eliminate the matrix effect. The result of the assay could be seen by eye under UV light excitation or registered by a portable power-dependent photometer. The limit of CAP detection by the second approach is 0.2 ng/mL, and the limit of quantitation is 0.3 ng/mL.
authors.affiliation: AN Baсh Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia. berlina.anna@gmail.com
authors.forename: Anna N
authors.initials: AN
authors.lastname: Berlina
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Taranova
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: Yuri Y
authors.initials: YY
authors.lastname: Vengerov
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D000818:Animals; D002417:Cattle; D002701:Chloramphenicol / Q000008:administration & dosage*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation*; D015200:Fluoroimmunoassay / Q000295:instrumentation*; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002701:Chloramphenicol