title: Dermatophagoides farinae allergens diversity identification by proteomics.
abstract: The most important indoor allergens for humans are house dust mites (HDM). Fourteen Dermatophagoides farinae allergens (Der f 1-3, 6, 7, 10, 11, 13-18, and 22) are reported although more than 30 allergens have been estimated in D. farinae. Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D. farina extracts. Their sequences were determined by Edman degradation, mass spectrometry analysis, and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests, immunoblots, basophil activation test, and skin prick tests. Eight of them are the first report as D. farinae allergens. The procedure of using a proteomic approach combined with a purely discovery approach using sera of patients with broad IgE reactivity profiles to mite allergens was an effective method to investigate a more complete repertoire of D. farinae allergens. The identification of eight new D. farinae allergens will be helpful for HDM allergy diagnosis and therapy, especially for patients without response for HDM major allergens. In addition, the current work significantly extendedthe repertoire of D. farinae allergens.
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.
authors.forename: Su
authors.initials: S
authors.lastname: An
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.forename: Chengbo
authors.initials: C
authors.lastname: Long
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Xu
authors.forename: Xingre
authors.initials: X
authors.lastname: Lu
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Rong
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D001249:Asthma / Q000097:blood / Q000276:immunology; D002648:Child; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D008875:Middle Aged; D008969:Molecular Sequence Data; D040901:Proteomics; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D007073:Immunoglobulin E