title: Mechanisms of intracerebral lymphoma growth delineated in a syngeneic mouse model of central nervous system lymphoma.
abstract: Primary lymphoma of the central nervous system (PCNSL) is defined as lymphoma of the diffuse large B-cell type confined to the CNS. To understand the effects of the CNS microenvironment on the malignant B cells and their interactions with the cells of the target organ, we analyzed a syngeneic mouse model. Transplantation of BAL17 cells into the frontal white matter of syngeneic BALB/c mice induced lymphomas with major clinical and neuropathologic features that parallel those of human PCNSL, including an angiocentric growth pattern in the brain parenchyma and tropism for the inner and outer ventricular system. Seven cycles of repeated isolation of lymphoma cells from the CNS and their intracerebral reimplantation induced genotypic and phenotypic alterations in resulting BAL17VII cells; the affected genes regulate apoptosis and are of the JAK/STAT pathway. Because lymphoma growth of BAL17VII cells was significantly accelerated, that is, shortening the time to death of the mice, these data indicate that prolonged stay of the lymphoma cells in the CNS was associated with worse outcome. These findings suggest that the CNS microenvironment fosters aggressiveness of lymphoma cells, thereby accelerating the lethal course of PCNSL.
authors.affiliation: Institute for Neuropathology, University Hospital of Cologne, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Montesinos-Rongen
authors.forename: Mónica
authors.initials: M
authors.lastname: Sánchez-Ruiz
authors.forename: Anna
authors.initials: A
authors.lastname: Brunn
authors.forename: Ke
authors.initials: K
authors.lastname: Hong
authors.forename: Susanne
authors.initials: S
authors.lastname: Bens
authors.forename: Sandra Rodriguez
authors.initials: SR
authors.lastname: Perales
authors.forename: Juan C
authors.initials: JC
authors.lastname: Cigudosa
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
authors.forename: Martina
authors.initials: M
authors.lastname: Deckert
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D016543:Central Nervous System Neoplasms / Q000235:genetics / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014185:Transplantation, Isogeneic