title: Vancomycin-chitosan composite deposited on post porous hydroxyapatite coated Ti6Al4V implant for drug controlled release.
abstract: Through the hydrogen bonds and the deprotonation, the vancomycin-chitosan composite has been originally deposited on Ti4Al4V by electrochemical technology. However, the rapid destruction of the hydrogen bonding between them by polar water molecules during immersion tests revealed 80% drug burst in a few hours. In this study, the post porous hydroxyapatite (HA) coated Ti4Al4V is prepared for the subsequent electrolytic deposition of vancomycin-chitosan composite to control the drug release. As expected, the initial burst is reduced to 55%, followed by a steady release about 20% from day 1 to day 5 and a slower release of the retained 25% after day 6, resulting in bacterial inhibition zone diameter of 30 mm which can last for more than a month in antibacterial tests, compared with the coated specimen without HA gradually loosing inhibition zone after 21 days. Besides, the cell culture indicates that the vancomycin-chitosan/HA composite coated has enhanced the proliferation, the differentiation and the mineralization of the osteoblast-like cell. In general, it is helpful for the osteointegration on permanent implants. Consistently, it effectively provides the prophylaxis and therapy of osteomyelitis according to the results of the rabbit infection animal model.
authors.affiliation: Department of Materials Science and Engineering, National Chung Hsing, University 250, Kuo-Kuang Road, Taichung 40227, Taiwan.
authors.forename: Chi-Chuan
authors.initials: CC
authors.lastname: Yang
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Lin
authors.forename: Jiunn-Wang
authors.initials: JW
authors.lastname: Liao
authors.forename: Shiow-Kang
authors.initials: SK
authors.lastname: Yen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000497:Alloys; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D048271:Chitosan / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003692:Delayed-Action Preparations; D017886:Durapatite / Q000737:chemistry*; D005269:Femur / Q000187:drug effects / Q000473:pathology; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D008826:Microbial Sensitivity Tests; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D016062:Porosity; D011817:Rabbits; D011859:Radiography; D013211:Staphylococcus aureus / Q000187:drug effects; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects / Q000601:surgery; D014025:Titanium / Q000494:pharmacology*; D014640:Vancomycin / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D015675:Osteocalcin; C031462:titanium alloy (TiAl6V4); D014640:Vancomycin; D048271:Chitosan; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase