title: Enzymatic synthesis of S-phenyl-L-cysteine from keratin hydrolysis industries wastewater with tryptophan synthase.
abstract: An economical method for production of S-phenyl-L-cysteine from keratin acid hydrolysis wastewater (KHW) containing L-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative KHW utilization strategy to synthesize S-phenyl-L-cysteine. Tryptophan synthase could efficiently convert L-serine contained in KHW to S-phenyl-L-cysteine at pH 9.0, 40°C and Trion X-100 of 0.02%. In a scale up study, L-serine conversion rate reach 97.1% with a final S-phenyl-L-cysteine concentration of 38.6 g l(-1).
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, PR China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Xu
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Pingting
authors.initials: P
authors.lastname: Mao
authors.forename: Junzhong
authors.initials: J
authors.lastname: Liu
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Qing-Cai
authors.initials: QC
authors.lastname: Jiao
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002855:Chromatography, Thin Layer; D003545:Cysteine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007220:Industrial Waste / Q000032:analysis*; D007633:Keratins / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D014367:Tryptophan Synthase / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000643:Ammonium Chloride; D003545:Cysteine; D007220:Industrial Waste; D007633:Keratins; D012694:Serine; D013501:Surface-Active Agents; D014367:Tryptophan Synthase; D062065:Wastewater; C042216:beta-phenylcysteine