title: Safety assessment and probiotic evaluation of Enterococcus faecium YF5 isolated from sourdough.
abstract: Enterococcus faecium YF5, a strain previously isolated from sourdough, was assessed for safety and probiotic potential. Its virulence and antibiotic resistant phenotypes (cytolysin and gelatinase production, antibiotic susceptibility) and genes (cylA, gelE, ace, agg, esp, and vanA) were surveyed. Results indicated that the tested virulence determinants were nontoxic. In addition, E. faecium YF5 was sensitive to 3 antibiotics such as amoxicillin, vancomycin, and chloramphenicol. Furthermore, results of in vivo animal acute oral toxicity of E. faecium YF5 studies were similar to the control group that indicated no abnormalities. In addition, E. faecium YF5 stably survived in low pH, bile salts, gastric, and intestinal fluids in vitro. Moreover, E. faecium YF5 was found to adhere to human colon cancer cell line HT-29 at 3.39 (±0.67) × 10(5) CFU/mL. When cocultured with pathogenic organisms (Enterobacter sakazakii CMCC45402, Escherichia coli CMCC44102, enterohemorrhage Escherichia coli O157: H7 CMCC44828, Salmonella Typhimurium CMCC50071, Shigella flexneri 301, and Shigella sonnei ATCC 29930) and 2 gram-positive strains (Listeria monocytogenes CMCC54001 and Staphylococcus aureus CMCC 26003), it inhibited these foodborne pathogens with exception of S. aureus. Therefore, E. faecium YF5 can be regarded as a safe strain and it may be used as a probiotic preparation or for microecologics.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, PR China.
authors.forename: Qianglai
authors.initials: Q
authors.lastname: Tan
authors.forename: Hengyi
authors.initials: H
authors.lastname: Xu
authors.forename: Zoraida P
authors.initials: ZP
authors.lastname: Aguilar
authors.forename: Shanshan
authors.initials: S
authors.lastname: Peng
authors.forename: Suqin
authors.initials: S
authors.lastname: Dong
authors.forename: Baogui
authors.initials: B
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Tingtao
authors.initials: T
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology; D000818:Animals; D000898:Antibiosis; D002701:Chloramphenicol / Q000494:pharmacology; D044083:Cronobacter sakazakii; D024881:Drug Resistance, Bacterial; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D019453:Escherichia coli O157; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D019073:HT29 Cells; D006801:Humans; D008089:Listeria monocytogenes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D019936:Probiotics; D012486:Salmonella typhimurium; D012763:Shigella flexneri; D012764:Shigella sonnei; D013211:Staphylococcus aureus; D014640:Vancomycin / Q000494:pharmacology; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors; D002701:Chloramphenicol; D014640:Vancomycin; D000658:Amoxicillin