title: A comparative study of anti-Candida activity and phenolic contents of the calluses from Lythrum salicaria L. in different treatments.
abstract: In the study, anti-Candida activity and phenol contents of Lythrum salicaria L. calli and wild species have been evaluated. The seeds of L. salicaria (Lythraceae), collected from Lahidjan City in the north of Iran, were cultured in Murashige and Skoog medium (MSM) with a supplement, gibberellin, to germinate. Callus inductions were performed from segments of seedling on MSM containing different concentrations of plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The activity of calluses extracts, wild plant, gallic acid, and 3,3',4'-tri-O-methylellagic acid-4-O-β-D-glucopyranoside (TMEG) as the main phenolic compounds against Candida albicans was assessed using cup plate diffusion method. The total phenols contents of calli and wild plant extracts were analyzed using Folin-Ciocalteu reagent. The callus formation in MSM supplemented with various concentrations of 2,4-D and BAP were 0-100 %. Anti-Candida activity of callus extract which obtained from MSM supplemented with 2,4-D and BAP (1 mgdm(-3)) was similar to the wild plant extract. Minimum inhibitory concentration values of gallic acid and TMEG were obtained as 0.312 and 2.5 mgcm(-3), respectively. Gallic acid equivalent values in all treatments were from 0 to 288 μg GAE mg(-1). Phenolic contents of plant aerial parts (331±3.7 μg GAE mg(-1)) and the callus, which developed in MSM including 1 mgdm(-3) of both 2,4-D and BAP, showed the same phenolic value and exhibited anti-Candida extract activity.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran 14174, Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Manayi
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Saeidnia
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Faramarzi
authors.forename: Nasrin
authors.initials: N
authors.lastname: Samadi
authors.forename: Samineh
authors.initials: S
authors.lastname: Jafari
authors.forename: Mahdi
authors.initials: M
authors.lastname: Vazirian
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Ghaderi
authors.forename: Tahmineh
authors.initials: T
authors.lastname: Mirnezami
authors.forename: Abbas
authors.initials: A
authors.lastname: Hadjiakhoondi
authors.forename: Mohammad Reza Shams
authors.initials: MR
authors.lastname: Ardekani
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Khanavi
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001593:Benzyl Compounds; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D004610:Ellagic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005707:Gallic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005875:Gibberellins / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007701:Kinetin / Q000494:pharmacology; D031561:Lythrum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D011687:Purines; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: C553569:3,3',4'-tri-O-methylellagic acid 4-O-beta-glucupyranuronide; D000935:Antifungal Agents; D001593:Benzyl Compounds; D005875:Gibberellins; D006027:Glycosides; D010936:Plant Extracts; D010937:Plant Growth Regulators; D011687:Purines; D004610:Ellagic Acid; D015084:2,4-Dichlorophenoxyacetic Acid; D005707:Gallic Acid; C480551:benzylaminopurine; D007701:Kinetin