title: Antifertility effect of hydroalcoholic leaves extract of Michelia champaca L.: an ethnomedicine used by Bhatra women in Chhattisgarh state of India.
abstract: BACKGROUND
  Michelia champaca L. (family: Magnoliaceae), commonly known as Champa [Hindi], is traditionally used for fertility regulation by the women of Chhattisgarh state in India. No scientific evidence regarding the antifertility effect of this plant is available till date.
  
  
  OBJECTIVE
  To study the anti-fertility effect of hydroalcoholic leaf extract of Michelia champaca Linn. in female rats.
  
  
  METHODS
  The antifertility activity of the extract (HAEMC) administered at dose levels (100 and 200mg/kg body weight, p.o.) was evaluated in two experimental animal models i.e. antiimplantation activity in female wistar rats and esterogenic/antiestrogenic activity in ovariectomized female rats. In anti-implantation activity, the extract (200 and 400mg/kg body weight, p.o.) was administered to female rats from 1 to 7 days of pregnancy and on 10th day, laprotomy was performed to count the no. of implants. For estrogenic/anti-estrogenic activity, ovariectomized female rats were administered with the extract at both the doses alone as well as along with 17α-ethinyl estradiol (1 μ/rat/day) for 7 consecutive days. On the 8th day, all animals were sacrificed and blood serum was further processed for the estimation of biochemical parameters such as estrogen level, alkaline phosphates, cholesterol, tryglycerides, total protein etc.
  
  
  RESULTS
  The extract (HAEMC) showed significant (p<0.01) 49.95% and 71.03% antiimplantation activities at 100 and 200mg/kg doses respectively. The extract also exhibited significant (p<0.01) estrogenic activity as evidenced by increase in body weight, uterine weight, increased thickness and height of endometrium, vaginal cornification and significant (p<0.01) increase in estrogen, cholesterol, alkaline phosphate and triglycerides levels at higher dose when administered alone as well as along with ethinyl estradiol. Phytochemical screening showed the presence of steroids, flavonoids and alkaloids in the extract.
  
  
  CONCLUSIONS
  Hydroalchoholic extract of Michelia champaca leaves possesses significant antifertility effect which might be due to the inhibition of implantation and estrogenic effect which in turn might be due to the presence of some phytoconstituents in the plant.
authors.affiliation: Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Taprial
authors.forename: Deepak
authors.initials: D
authors.lastname: Kashyap
authors.forename: Vineet
authors.initials: V
authors.lastname: Mehta
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
mesh_terms: D000818:Animals; D003270:Contraceptive Agents / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007194:India; D029562:Magnoliaceae; D008297:Male; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D003270:Contraceptive Agents; D004965:Estrogen Antagonists; D004967:Estrogens; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol