title: A homogeneous chemiluminescent immunoassay method.
abstract: A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.
authors.affiliation: Beckman Coulter Inc., Southfield, Michigan 48033, USA. hakhavantafti@beckman.com
authors.forename: Hashem
authors.initials: H
authors.lastname: Akhavan-Tafti
authors.forename: Dean G
authors.initials: DG
authors.lastname: Binger
authors.forename: John J
authors.initials: JJ
authors.lastname: Blackwood
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Richard S
authors.initials: RS
authors.lastname: Creager
authors.forename: Renuka
authors.initials: R
authors.lastname: de Silva
authors.forename: Robert A
authors.initials: RA
authors.lastname: Eickholt
authors.forename: Jose E
authors.initials: JE
authors.lastname: Gaibor
authors.forename: Richard S
authors.initials: RS
authors.lastname: Handley
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Kapsner
authors.forename: Senja K
authors.initials: SK
authors.lastname: Lopac
authors.forename: Michael E
authors.initials: ME
authors.lastname: Mazelis
authors.forename: Terri L
authors.initials: TL
authors.lastname: McLernon
authors.forename: James D
authors.initials: JD
authors.lastname: Mendoza
authors.forename: Bruce H
authors.initials: BH
authors.lastname: Odegaard
authors.forename: Sarada G
authors.initials: SG
authors.lastname: Reddy
authors.forename: Michael
authors.initials: M
authors.lastname: Salvati
authors.forename: Barry A
authors.initials: BA
authors.lastname: Schoenfelner
authors.forename: Nir
authors.initials: N
authors.lastname: Shapir
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Shelly
authors.forename: Jeff C
authors.initials: JC
authors.lastname: Todtleben
authors.forename: Guoping
authors.initials: G
authors.lastname: Wang
authors.forename: Wenhua
authors.initials: W
authors.lastname: Xie
mesh_terms: D000166:Acridines / Q000737:chemistry*; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D000242:Cyclic AMP / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D016209:Interleukin-8 / Q000032:analysis; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D017430:Prostate-Specific Antigen / Q000032:analysis; D051381:Rats; D012756:Sheep
chemical_list: D000166:Acridines; D055910:Antibodies, Immobilized; D016209:Interleukin-8; D049408:Luminescent Agents; C011633:acridan; D000242:Cyclic AMP; D006735:Horseradish Peroxidase; D017430:Prostate-Specific Antigen