title: A first AFLP-based genetic linkage map for brine shrimp Artemia franciscana and its application in mapping the sex locus.
abstract: We report on the construction of sex-specific linkage maps, the identification of sex-linked markers and the genome size estimation for the brine shrimp Artemia franciscana. Overall, from the analysis of 433 AFLP markers segregating in a 112 full-sib family we identified 21 male and 22 female linkage groups (2n = 42), covering 1,041 and 1,313 cM respectively. Fifteen putatively homologous linkage groups, including the sex linkage groups, were identified between the female and male linkage map. Eight sex-linked AFLP marker alleles were inherited from the female parent, supporting the hypothesis of a WZ-ZZ sex-determining system. The haploid Artemia genome size was estimated to 0.93 Gb by flow cytometry. The produced Artemia linkage maps provide the basis for further fine mapping and exploring of the sex-determining region and are a possible marker resource for mapping genomic loci underlying phenotypic differences among Artemia species.
authors.affiliation: Laboratory of Aquaculture, Artemia Reference Center, Department of Animal Production, Ghent University, Gent, Belgium.
authors.forename: Stephanie
authors.initials: S
authors.lastname: De Vos
authors.forename: Peter
authors.initials: P
authors.lastname: Bossier
authors.forename: Gilbert
authors.initials: G
authors.lastname: Van Stappen
authors.forename: Ilse
authors.initials: I
authors.lastname: Vercauteren
authors.forename: Patrick
authors.initials: P
authors.lastname: Sorgeloos
authors.forename: Marnik
authors.initials: M
authors.lastname: Vuylsteke
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001156:Artemia / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D059646:Genome Size; D008297:Male; D018895:Microsatellite Repeats; D012727:Sex Characteristics; D019849:Sex Determination Processes