title: Backbone ¹H, ¹³C and ¹⁵N resonance assignment of the C-terminal EGF-cbEGF pair of LTBP1 and flanking residues.
abstract: Latent TGFβ binding protein 1 (LTBP1) is a large extracellular protein that has been shown to bind covalently to the propeptide of TGFβ cytokines and form a large latent complex, which is then incapable of binding TGFβ receptors. LTBP1 has also been demonstrated to interact with a number of insoluble extracellular matrix components, such as fibrillin, which may play a role in TGFβ regulation. Here we present the backbone (1)H, (13)C and (15)N assignments for two EGF domains of human LTBP1, and flanking regions, together forming a 12 kDa protein fragment at the C-terminus of LTBP1. This region is of particular interest as it is postulated to be involved in interactions with fibrillin microfibrils.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
authors.forename: Ian B
authors.initials: IB
authors.lastname: Robertson
authors.forename: Penny A
authors.initials: PA
authors.lastname: Handford
authors.forename: Christina
authors.initials: C
authors.lastname: Redfield
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism*; D002247:Carbon Isotopes; D004815:Epidermal Growth Factor / Q000737:chemistry*; D006801:Humans; D006859:Hydrogen; D053779:Latent TGF-beta Binding Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D053779:Latent TGF-beta Binding Proteins; D009587:Nitrogen Isotopes; D004815:Epidermal Growth Factor; D006859:Hydrogen; D002118:Calcium