title: HPV testing by cobas HPV test in a population from Catalonia.
abstract: BACKGROUND
  HPV testing in cervical cancer screening has been proposed as an alternative or complementary to cytology in women older than 30 years. However, adequate clinical sensitivity and specificity are crucial for a new test to be implemented. Hybrid Capture 2 (HC2) has proved good clinical performance in selecting women at risk for high-grade intraepithelial lesions with a high sensitivity and specificity. cobas HPV Test has been recently launched and its performance in different clinical settings needs to be determined.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the cobas HPV Test for the detection of cervical HPV infection in a population of women in Catalonia (Spain) using HC2 as a reference.
  
  
  METHODS
  Cervical liquid cytology samples from 958 women have been studied. Sensitivity was analyzed in 60 samples from patients with a high-grade intraepithelial lesion (≥ CIN2) on histology and specificity was determined in 898 samples from women with no ≥ CIN2. All cases had HC2 and cobas HPV Test performed. Statistical analyses of sensitivity, specificity and comparison between HC2 and cobas HPV Test by a non-inferiority test were applied.
  
  
  RESULTS
  Sensitivity of HC2 and cobas HPV Test for detecting ≥ CIN2 proved identical (98.3%) while specificity was 85.3% and 86.2% respectively. The non-inferiority test demonstrated that cobas HPV Test surpassed 90% sensitivity and 98% specificity of HC2.
  
  
  CONCLUSIONS
  The cobas HPV Test results fulfilled sensitivity and specificity requirements for HPV based cervical cancer screening and for the triage of minor cytological abnormalities, allowing its introduction in clinical settings.
authors.affiliation: Department of Pathology, Hospital del Mar, Universitat Autònoma de Barcelona, Barcelona, Spain. blloveras@parcdesalutmar.cat
authors.forename: Belén
authors.initials: B
authors.lastname: Lloveras
authors.forename: Silvia
authors.initials: S
authors.lastname: Gomez
authors.forename: Francesc
authors.initials: F
authors.lastname: Alameda
authors.forename: Beatriz
authors.initials: B
authors.lastname: Bellosillo
authors.forename: Sergi
authors.initials: S
authors.lastname: Mojal
authors.forename: Mercè
authors.initials: M
authors.lastname: Muset
authors.forename: Manuel
authors.initials: M
authors.lastname: Parra
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Palomares
authors.forename: Sergi
authors.initials: S
authors.lastname: Serrano
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D061809:Human Papillomavirus DNA Tests / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology*; D012680:Sensitivity and Specificity; D013030:Spain / Q000453:epidemiology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000821:virology*