title: Simple method for differentiating measles vaccine from wild-type strains using loop-mediated isothermal amplification.
abstract: Because of increasing measles vaccine coverage, the proportion of patients with modified measles has been increasing. Such patients have low-grade fever with very mild eruptions similar to vaccine-related adverse events. Differentiation between these two pathogenic conditions is required to improve the quality of laboratory-based measles surveillance. In this study, vaccine-specific and wild-type specific primer sets were designed for loop-mediated isothermal amplification in the N gene, and vaccine strains, C1, D3, D4, D5, D8, D9, G3 and H1 wild strains were examined. Three vaccine strains were efficiently amplified using a vaccine-specific primer set with an approximately 10-times higher sensitivity than wild-type primer. Modified measles was differentiated from vaccine-associated cases by this system, but limitations were encountered with the other genotypes.
authors.affiliation: Laboratory of Virus Infection, Kitasato Institute for Life Sciences, Shirokane 5-9-1, Minato-ku, Tokyo, 108-8641, Japan. tetsuo‐n@lisci.kitasato‐u.ac.jp
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nakayama
authors.forename: Akihito
authors.initials: A
authors.lastname: Sawada
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kubo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kaida
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Naoki
authors.initials: N
authors.lastname: Shigemoto
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Komase
authors.forename: Makoto
authors.initials: M
authors.lastname: Takeda
mesh_terms: D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008457:Measles / Q000175:diagnosis* / Q000821:virology*; D008458:Measles Vaccine / Q000235:genetics* / Q000276:immunology; D008459:Measles virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D017931:DNA Primers; D008458:Measles Vaccine