title: Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice.
abstract: Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (α-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.
authors.affiliation: Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: K W
authors.initials: KW
authors.lastname: Wang
authors.forename: L L
authors.initials: LL
authors.lastname: Chueh
authors.forename: M H
authors.initials: MH
authors.lastname: Wang
authors.forename: Y T
authors.initials: YT
authors.lastname: Huang
authors.forename: B H
authors.initials: BH
authors.lastname: Fang
authors.forename: C Y
authors.initials: CY
authors.lastname: Chang
authors.forename: M C
authors.initials: MC
authors.lastname: Fang
authors.forename: J Y
authors.initials: JY
authors.lastname: Chou
authors.forename: S C
authors.initials: SC
authors.lastname: Hsieh
authors.forename: C H
authors.initials: CH
authors.lastname: Wan
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000821:virology; D017992:Parvovirus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012376:Rodent Diseases / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D014764:Viral Proteins