title: A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs.
abstract: The aim of the study was to evaluate the effect of a cell-free hyaluronate/type I collagen/fibrin composite scaffold containing polyvinyl alcohol (PVA) nanofibers enriched with liposomes, basic fibroblast growth factor (bFGF) and insulin on the regeneration of osteochondral defects. A novel drug delivery system was developed on the basis of the intake effect of liposomes encapsulated in PVA nanofibers. Time-controlled release of insulin and bFGF improved MSC viability in vitro. Nanofibers functionalized with liposomes also improved the mechanical characteristics of the composite gel scaffold. In addition, time-controlled release of insulin and bFGF stimulated MSC recruitment from bone marrow in vivo. Cell-free composite scaffolds containing PVA nanofibers enriched with liposomes, bFGF, and insulin were implanted into seven osteochondral defects of miniature pigs. Control defects were left untreated. After 12 weeks, the composite scaffold had enhanced osteochondral regeneration towards hyaline cartilage and/or fibrocartilage compared with untreated defects that were filled predominantly with fibrous tissue. The cell-free composite scaffold containing PVA nanofibers, liposomes and growth factors enhanced migration of the cells into the defect, and their differentiation into chondrocytes; the scaffold was able to enhance the regeneration of osteochondral defects in minipigs.
authors.affiliation: Institute of Experimental Medicine of the ASCR, Vídenská 1083, 142 20 Prague, Czech Republic. evafil@biomed.cas.cz
authors.forename: Eva
authors.initials: E
authors.lastname: Filová
authors.forename: Michala
authors.initials: M
authors.lastname: Rampichová
authors.forename: Andrej
authors.initials: A
authors.lastname: Litvinec
authors.forename: Milan
authors.initials: M
authors.lastname: Držík
authors.forename: Andrea
authors.initials: A
authors.lastname: Míčková
authors.forename: Matej
authors.initials: M
authors.lastname: Buzgo
authors.forename: Eva
authors.initials: E
authors.lastname: Košťáková
authors.forename: Lenka
authors.initials: L
authors.lastname: Martinová
authors.forename: Dušan
authors.initials: D
authors.lastname: Usvald
authors.forename: Eva
authors.initials: E
authors.lastname: Prosecká
authors.forename: Jiří
authors.initials: J
authors.lastname: Uhlík
authors.forename: Jan
authors.initials: J
authors.lastname: Motlík
authors.forename: Luděk
authors.initials: L
authors.lastname: Vajner
authors.forename: Evžen
authors.initials: E
authors.lastname: Amler
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D002454:Cell Differentiation; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology; D024042:Collagen Type I / Q000737:chemistry; D055119:Elastic Modulus; D005260:Female; D005337:Fibrin / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D006820:Hyaluronic Acid / Q000737:chemistry; D007328:Insulin / Q000008:administration & dosage*; D008081:Liposomes; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D057139:Nanofibers / Q000008:administration & dosage* / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds
chemical_list: D024042:Collagen Type I; D007328:Insulin; D008081:Liposomes; D016222:Fibroblast Growth Factor 2; D005337:Fibrin; D011142:Polyvinyl Alcohol; D006820:Hyaluronic Acid