title: Expression and purification of biologically active human FGF2 containing the b'a' domains of human PDI in Escherichia coli.
abstract: Among the members of the fibroblast growth factor (FGF) family that affect the growth, differentiation, migration, and survival of many cell types, FGF2 is the most abundant in the central nervous system. Because of its wound healing effects, FGF2 has potential as a therapeutic agent. The protein is also added to the culture media to maintain stem cells. Expression and purification procedures for FGF2 that are highly efficient and low cost have been intensively investigated for the past two decades. Our current study focuses on the purification of FGF2 fused with b'a' domains of human protein disulfide isomerase to elevate overexpression, solubility, and stability with a simplified experimental procedure using only ion exchange chromatography, as well as on the confirmation of the biological activity of FGF2 on fibroblast Balb/c 3T3 cells and hippocampal neural cells.
authors.affiliation: Department of Physiology and Bio-medical Institute of Technology, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
authors.forename: Jung-A
authors.initials: JA
authors.lastname: Song
authors.forename: Bon-Kyung
authors.initials: BK
authors.lastname: Koo
authors.forename: Seon Ha
authors.initials: SH
authors.lastname: Chong
authors.forename: Jihye
authors.initials: J
authors.lastname: Kwak
authors.forename: Han-Bong
authors.initials: HB
authors.lastname: Ryu
authors.forename: Minh Tan
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Thu Trang Thi
authors.initials: TT
authors.lastname: Vu
authors.forename: Boram
authors.initials: B
authors.lastname: Jeong
authors.forename: Seong Who
authors.initials: SW
authors.lastname: Kim
authors.forename: Han
authors.initials: H
authors.lastname: Choe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D015870:Gene Expression; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010957:Plasmids; D019704:Protein Disulfide-Isomerases / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D011993:Recombinant Fusion Proteins; D016222:Fibroblast Growth Factor 2; D019704:Protein Disulfide-Isomerases