title: Interaction of curcumin with phosphocasein micelles processed or not by dynamic high-pressure.
abstract: The binding of curcumin to native-like phosphocaseins (PC) dispersed in simulated milk ultrafiltrate at pH 6.6 was assessed by fluorescence spectrophotometry. Curcumin binds to native-like PC micelles with ∼1 binding site per casein molecule, and a binding constant of 0.6-5.6 × 10(4)M(-1). Dynamic high pressure (or ultra-high pressure homogenisation, UHPH) at 200 MPa did not affect the binding parameters of curcumin to processed PC. UHPH-processing of PC dispersions at 300 MPa was followed by a slight but significant (p=0.05) increase in the binding constant of curcumin to processed PC, which may result from the significant UHPH-induced dissociation of initial PC micelles into neo-micelles of smaller sizes, and from the corresponding 1.5-2-fold increase in micelle surface area. PC-curcumin complexes were resistant to pepsin but were degraded by pancreatin, providing the possibility of a spatiotemporally controlled release and protection of bound biomolecules. UHPH-processed PC did not induce TC7-cell damage or major inflammation as assessed by LDH release or IL-8 secretion, respectively, compared with native-like PC. PC micelles could provide a valuable submicron system to vectorise drugs and nutrients.
authors.affiliation: Université Montpellier 2, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, Equipe de Biochimie et Technologie Alimentaires cc023, 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: Amal
authors.initials: A
authors.lastname: Benzaria
authors.forename: Marc
authors.initials: M
authors.lastname: Maresca
authors.forename: Nadira
authors.initials: N
authors.lastname: Taieb
authors.forename: Eliane
authors.initials: E
authors.lastname: Dumay
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002460:Cell Line; D003474:Curcumin / Q000737:chemistry* / Q000493:pharmacokinetics; D004063:Digestion; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods*; D016503:Drug Delivery Systems; D004355:Drug Stability; D006801:Humans; D007700:Kinetics; D008823:Micelles; D008954:Models, Biological; D011312:Pressure; D011485:Protein Binding
chemical_list: D002364:Caseins; D004337:Drug Carriers; D008823:Micelles; D003474:Curcumin