title: Evaluation of some selected blood parameters and histopathology of liver and kidney of rats fed protein-substituted mucuna flour and derived protein rich product.
abstract: This comparative study reports the nutritional and toxicological characteristics of Mucuna pruriens flour and a protein-rich product developed from it. The protein-rich mucuna product (PRMP) was obtained by the three steps procedure: protein solubilization, heat-coagulation and sieving. Three weeks rats (n=6 per group) were fed for 28 days on standard protein-substituted rat feed with mucuna flour or PRMP. The experimental design was a factorial design with three mucuna accessions (Velvet, Black and White) and two treatments (flour and PRMP). The protein content ranged 27.2-31.5 g/100 g for flour and 58.8-61.1% for PRMP. Processing flour into PRMP led to a significant (p<0.05) reduction of tannins (50%), total polyphenols (50%) and trypsin inhibitors (94%). The rats fed PMRP diets witnessed weight gain similar to casein, while those fed mucuna flour lost weight. The levels of total cholesterol, HDL-cholesterol and LDL-cholesterol observed in animals groups fed mucuna flour and PRMP were significantly lower (p<0.05) than the control group. In addition lymphocytes, granulocytes, red blood cells, hemoglobin and hematocrit of rats fed mucuna flour were significantly (p<0.05) lower than values in other rats groups. Kidneys glomerular sclerosis and high creatinine levels were observed in group fed mucuna flour. PRMP then represents a good alternative of using mucuna proteins for human nutrition.
authors.affiliation: Department of Food Science and Nutrition, National School of Agro-Industrial Science, PO Box 455, Ngaoundere, Cameroon. josianetheresa@yahoo.fr
authors.forename: Josiane Therese Metsagang
authors.initials: JT
authors.lastname: Ngatchic
authors.forename: Selestion Dongmo
authors.initials: SD
authors.lastname: Sokeng
authors.forename: Nicolas Yanou
authors.initials: NY
authors.lastname: Njintang
authors.forename: Theophile
authors.initials: T
authors.lastname: Maoundombaye
authors.forename: Julius
authors.initials: J
authors.lastname: Oben
authors.forename: Carl Moses F
authors.initials: CM
authors.lastname: Mbofung
mesh_terms: D000821:Animal Feed; D000818:Animals; D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005433:Flour / Q000032:analysis*; D005511:Food Handling / Q000379:methods; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D031299:Mucuna / Q000737:chemistry; D014674:Plant Proteins, Dietary / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain / Q000187:drug effects
chemical_list: D008078:Cholesterol, LDL; D014674:Plant Proteins, Dietary; D002784:Cholesterol