title: Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approaches.
abstract: The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety of DNA assembly techniques to be organized for complex constructions (with or without scars). The assembly of five DNA fragments into a host genome was performed as an experimental demonstration.
authors.affiliation: School of Chemistry, University of Melbourne, Parkville, Victoria, Australia. shiz@student.unimelb.edu.au
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Shi
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Wedd
authors.forename: Sally L
authors.initials: SL
authors.lastname: Gras
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D023061:Gene Order; D005822:Genetic Vectors; D011995:Recombination, Genetic
chemical_list: D004247:DNA