title: Exploring the synthetic potential of cell bound β-glycosidase of Pichia etchellsii.
abstract: Enzymatic synthesis of oligosaccharides with absolute stereo-selectivity and regio-selectivity provides an economical alternative to classical chemical methods. Here we demonstrate, for the first time, that whole cells of P. etchellsii are highly efficient biocatalysts and can be used for oligosaccharide synthesis using p-nitrophenyl-β-D-glucopyranoside, o-nitrophenyl-β-D-glucopyranoside and p-nitrophenyl-β-D-xylopyranoside as both donors and acceptors. Auto-condensation of p-nitrophenyl-β-D-glucopyranoside and o-nitrophenyl-β-D-glucopyranoside resulted in formation of β-(1→6) linked disaccharide as major products in 4 and 12% yield respectively. By contrast, auto condensation of p-nitrophenyl-β-D-xylopyranoside exclusively lead to formation of β-(1→4) linked disaccharide in 24% yield.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, India.
authors.forename: Mohd Younis
authors.initials: MY
authors.lastname: Rather
authors.forename: Saroj
authors.initials: S
authors.lastname: Mishra
authors.forename: Subrayashastry
authors.initials: S
authors.lastname: Aravinda
mesh_terms: D005960:Glucosides / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry; D006801:Humans; D009844:Oligosaccharides / Q000096:biosynthesis*; D010843:Pichia / Q000201:enzymology* / Q000235:genetics
chemical_list: D005960:Glucosides; D006027:Glycosides; D009844:Oligosaccharides; C008646:4-nitrophenyl beta-D-xyloside; C025193:4-nitrophenyl beta-D-glucoside; D006026:Glycoside Hydrolases