title: Does prolonged pneumoperitoneum affect the kidney? Oxidative stress, stereological and electron microscopy study in a rat model.
abstract: OBJECTIVE
  Pneumoperitoneum (Pp) at 12 to 15 mmHg in rats is associated with kidney damage. However, Pp at 8 mmHg is now known to best correlate to working pressures used in humans. Thus the aim of this work was to study the kidney of rats submitted to prolonged Pp at 8 mmHg.
  
  
  METHODS
  Rats were divided into a Sham group (n = 14), submitted to anesthesia, and a Pp group (n = 14), submitted to Pp at 8 mmHg, followed by deflation. In both groups, 7 animals were immediately killed and their kidneys were used for oxidative stress analyses. The remaining 7 rats in each group were evaluated after 6 weeks for the number of glomeruli and podocyte morphology.
  
  
  RESULTS
  For all analyzed parameters Sham and Pp groups presented no statistical difference.
  
  
  CONCLUSIONS
  When submitted to adequate Pp pressures (8 mmHg), no kidney damage occurs in rats.
authors.affiliation: Urogenital Research Unit, State University of Rio de Janeiro, RJ 20551-030, Brazil. diogobenchimol@gmail.com
authors.forename: Diogo B de
authors.initials: DB
authors.lastname: Souza
authors.forename: Waldemar S
authors.initials: WS
authors.lastname: Costa
authors.forename: Luiz E M
authors.initials: LE
authors.lastname: Cardoso
authors.forename: Marlene
authors.initials: M
authors.lastname: Benchimol
authors.forename: Marco A
authors.initials: MA
authors.lastname: Pereira-Sampaio
authors.forename: Francisco J B
authors.initials: FJ
authors.lastname: Sampaio
mesh_terms: D000818:Animals; D007668:Kidney / Q000293:injuries* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D023421:Models, Animal; D009929:Organ Size; D018384:Oxidative Stress; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011312:Pressure / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013997:Time Factors
chemical_list: D008315:Malondialdehyde