title: A Magneto-Nanosensor Immunoassay for Sensitive Detection of Aspergillus Fumigatus Allergen Asp f 1.
abstract: We report a magneto-nanosensor biochip for fungal detection. The chip is made of arrays of giant magnetoresistive (GMR) spin-valve sensors, and is able to detect protein biomarkers at low concentrations in solutions. As a demonstration, a standard curve for fungal pathogen Asp f 1 was obtained by measuring signals from various concentrations of Asp f 1 spiked in PBS solutions, indicating a detection limit of ~100 pg/ml. Five positive and negative Asp f 1 solution samples were discriminated correctly in blind experiments. Our data suggest that the magneto-nanosensor biochips are very promising as sensitive diagnostic devices for fungal pathogens. Given the generality of the detection scheme used in the magneto-nanosensor, we anticipate that the platform will be very useful for the detection of many types of biomarkers.
authors.affiliation: Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305 USA.
authors.forename: Dokyoon
authors.initials: D
authors.lastname: Kim
authors.forename: Shan X
authors.initials: SX
authors.lastname: Wang
keywords: biochip; immunoassay; magnetic nanoparticles; magneto-nanosensor