title: Isolation and identification of bacteriocinogenic strain of Lactobacillus plantarum with potential beneficial properties from donkey milk.
abstract: OBJECTIVE
  The goal of this study was to isolate and characterize a lactic acid bacteria (LAB) from donkey milk with potential beneficial properties.
  
  
  RESULTS
  Lactic acid bacteria were isolated from donkey milk and identified based on physiological, biochemical and molecular methods. The isolate that presented highest bacteriocin potential (Lactobacillus plantarum LP08AD) was evaluated for the production of bacteriocin, including stability in the presence of various enzymes, surfactants, salts, pH and temperatures. Bactericidal effect of bacteriocin LP08AD on Listeria monocytogenes, Enterococcus faecium and Lactobacillus curvatus was shown for actively growing and stationary cells. Similar growth and bacteriocin production were observed when strain LP08AD was cultured in MRS broth at 30°C or 37°C. Bacteriocin LP08AD adhered at low levels on the producer cells (200 AU ml(-1) ). The presence of plantaricin W gene on the genomic DNA was recorded based on PCR. Good growth for strain LP08AD was recorded in MRS broth with pH from 5·0 to 9·0 and LP08AD grew well in the absence of oxbile or concentration below 0·8%. Lact. plantarum LP08AD was applied to the small intestinal epithelial polarized monolayers of H4, PSIc1 and CLAB and demonstrated low attachment ability on all cell lines studied, with values with a similar behaviour for cells from human and pig origin.
  
  
  CONCLUSIONS
  Bacteriocin-producing Lact. plantarum LP08AD might be useful in the design of novel functional foods with potential probiotic or biopreservation properties.
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first report on detection and characterization of bacteriocinogenic Lact. plantarum from donkey milk. The strain LP08AD shows to have potential beneficial properties, as demonstrated by the use of noncancerogenic cell lines.
authors.affiliation: Department of Agriculture and Life Sciences, Faculty of Medicine, University of Maribor, Hoče, Slovenia.
authors.forename: A
authors.initials: A
authors.lastname: Murua
authors.forename: S D
authors.initials: SD
authors.lastname: Todorov
authors.forename: A D S
authors.initials: AD
authors.lastname: Vieira
authors.forename: R C R
authors.initials: RC
authors.lastname: Martinez
authors.forename: A
authors.initials: A
authors.lastname: Cencič
authors.forename: B D G M
authors.initials: BD
authors.lastname: Franco
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D002460:Cell Line; D018397:Equidae / Q000382:microbiology; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008892:Milk / Q000382:microbiology; D019936:Probiotics; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins