title: The centrosomal E3 ubiquitin ligase FBXO31-SCF regulates neuronal morphogenesis and migration.
abstract: Neuronal development requires proper migration, polarization and establishment of axons and dendrites. Growing evidence identifies the ubiquitin proteasome system (UPS) with its numerous components as an important regulator of various aspects of neuronal development. F-box proteins are interchangeable subunits of the Cullin-1 based E3 ubiquitin ligase, but only a few family members have been studied. Here, we report that the centrosomal E3 ligase FBXO31-SCF (Skp1/Cullin-1/F-box protein) regulates neuronal morphogenesis and axonal identity. In addition, we identified the polarity protein Par6c as a novel interaction partner and substrate targeted for proteasomal degradation in the control of axon but not dendrite growth. Finally, we ascribe a role for FBXO31 in dendrite growth and neuronal migration in the developing cerebellar cortex. Taken together, we uncovered the centrosomal E3 ligase FBXO31-SCF as a novel regulator of neuronal development.
authors.affiliation: Cellular and Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Göttingen, Germany.
authors.forename: Mayur
authors.initials: M
authors.lastname: Vadhvani
authors.forename: Nicola
authors.initials: N
authors.lastname: Schwedhelm-Domeyer
authors.forename: Chaitali
authors.initials: C
authors.lastname: Mukherjee
authors.forename: Judith
authors.initials: J
authors.lastname: Stegmüller
mesh_terms: D001483:Base Sequence; D002465:Cell Movement; D018385:Centrosome / Q000201:enzymology*; D017931:DNA Primers; D018274:Electroporation; D044783:F-Box Proteins / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D009024:Morphogenesis; D009474:Neurons / Q000166:cytology*; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D017931:DNA Primers; D044783:F-Box Proteins; C507917:FBXO31 protein, human; D025521:Tumor Suppressor Proteins; D044843:SKP Cullin F-Box Protein Ligases