title: [Effects of daunorubicin on KG1a cell proliferation and Eps8 expression].
abstract: The aim of this study was to observe the inhibitory effect of daunorubicin on KG1a cells and the expression of Eps8 which is a novel tumor-associated antigen with its full name epidermal growth factor receptor pathway substrate 8 (Eps8), and to explore the effect of daunorubicin on Eps8 expression in KG1a cells at mRNA and protein levels. The KG1a cells were treated with different concentration of daunorubicin for 24, 48, 72 h, then trypan blue staining was used to detect the inhibitory rate of KGla cells, RQ-PCR and Western blot were used to detect Eps8 mRNA and Eps8 protein expression. The results showed that daunorubicin inhibited the proliferation of KG1a cells in a dose and time dependent manner (r = 0.983, P < 0.01). Daunorubicin could reduce the mRNA and protein levels of Eps8 expression in dose and time dependent manners in KG1a cells (r = 0.979, P < 0.05). It is concluded that with the increasing of concentration and time of daunorubicin acting on KG1a cells, the cell proliferative inhibitory effect increased and the expression of Eps8 decreased, suggesting that the inhibitory effect of daunorubicin on KG1a cell proliferation is realized through downregulation of Eps8 expression.
authors.affiliation: Department of Hematology, Southern Medical University, Guangdong Province, China.
authors.forename: Jin-Ying
authors.initials: JY
authors.lastname: Gan
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Xiong
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Song-Hao
authors.initials: SH
authors.lastname: Cai
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003630:Daunorubicin / Q000494:pharmacology*; D006801:Humans
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C489989:EPS8 protein, human; D003630:Daunorubicin