title: Fertility of cryopreserved ovine semen is determined by sperm velocity.
abstract: The present study aims to examine the predictive value of some sperm parameters on male fertility. Semen samples from six Manchega rams were collected and cryopreserved. Sperm quality was assessed after thawing and after 2h of incubation, either in the freezing extender (37°C) or after dilution in Synthetic Oviductal Fluid (SOF) (38°C, 5% CO2), attempting to mimic the physiological conditions of the female reproductive tract. The following sperm parameters were evaluated: motility and kinetic parameters by computer-assisted semen analyzer (CASA), and sperm viability (propidium iodide), mitochondrial membrane potential (JC-1), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), and intracellular calcium (fluo-3) by flow cytometry. Results showed no significant differences between incubation media neither after thawing nor after incubation. There were no significant correlations between fertility and sperm parameters assessed by flow cytometry. However, after incubation in the freezing extender, sperm samples from males with poor fertility yielded less linearity and velocity (P<0.05) as indicated by motility parameters analyzed by CASA. These results indicate that kinematic sperm motility parameters evaluation by CASA might be useful to identify samples with poor fertility.
authors.affiliation: SaBio IREC (CSIC - UCLM - JCCM), Campus Universitario s. n. 02071 Albacete, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Del Olmo
authors.affiliation: SaBio IREC (CSIC - UCLM - JCCM), Campus Universitario s. n. 02071 Albacete, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Bisbal
authors.affiliation: SaBio IREC (CSIC - UCLM - JCCM), Campus Universitario s. n. 02071 Albacete, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Maroto-Morales
authors.affiliation: SaBio IREC (CSIC - UCLM - JCCM), Campus Universitario s. n. 02071 Albacete, Spain.
authors.forename: O
authors.initials: O
authors.lastname: García-Alvarez
authors.affiliation: Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM, 13300 Valdepeñas, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Ramon
authors.affiliation: Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM, 13300 Valdepeñas, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Jimenez-Rabadan
authors.affiliation: ITRA-ULE, INDEGSAL, University of León, León, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Martínez-Pastor
authors.affiliation: SaBio IREC (CSIC - UCLM - JCCM), Campus Universitario s. n. 02071 Albacete, Spain.
authors.forename: A J
authors.initials: AJ
authors.lastname: Soler
authors.affiliation: SaBio IREC (CSIC - UCLM - JCCM), Campus Universitario s. n. 02071 Albacete, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Garde
authors.affiliation: SaBio IREC (CSIC - UCLM - JCCM), Campus Universitario s. n. 02071 Albacete, Spain. Electronic address: MRocio.Fernandez@uclm.es.
authors.forename: M R
authors.initials: MR
authors.lastname: Fernandez-Santos
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000032:analysis; D015925:Cryopreservation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005298:Fertility / Q000502:physiology*; D005434:Flow Cytometry / Q000662:veterinary; D016014:Linear Models; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012756:Sheep / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D002118:Calcium