title: Control of extravillous trophoblast function by the eotaxins CCL11, CCL24 and CCL26.
abstract: OBJECTIVE
  What are the effects of the eotaxin group of chemokines (CCL11, CCL24 and CCL26) on extravillous trophoblast (EVT) functions important during uterine decidual vessel remodelling?
  
  
  CONCLUSIONS
  CCL11, CCL24 and CCL26 can regulate EVT migration, invasion and adhesion, highlighting a potential regulatory role for these chemokines during uterine decidual spiral arteriole remodelling in the first trimester of human pregnancy.
  
  
  BACKGROUND
  A successful human pregnancy depends on adequate remodelling of the uterine decidual spiral arterioles, a process carried out by EVT which invade from the placenta. The invasion by EVT into the maternal uterine decidual vessels is regulated by the interaction of many factors including members of the chemokine subfamily of cytokines.
  
  
  METHODS
  This study used the HTR8/SVneo cell line as a model for invasive EVT. All experiments were repeated on at least three separate occasions.
  
  
  METHODS
  The effect of recombinant human CCL11, CCL24 and CCL26 on EVT migration and invasive potential was measured using the xCELLigence real-time system, wound-healing and Matrigel invasion assays, zymography to measure MMP activity and reverse zymography to measure TIMP activity. A commercially available adhesion assay was used to assess EVT adhesion to extracellular matrix proteins.
  
  
  RESULTS
  All the three eotaxins were found to significantly stimulate migration of the EVT-derived cell line HTR8/SVneo (P < 0.05) with no significant changes in cell number following treatment with each chemokine (P > 0.05). All the three eotaxins significantly increased HTR8/SVneo invasion (P < 0.05) and MMP2 activity (P < 0.05) without any effects on TIMP2 activity (P > 0.05). All the three eotaxins significantly increased HTR8/SVneo cell binding to collagen IV (P < 0.05) and fibronectin (P < 0.05).
  
  
  CONCLUSIONS
  This work has been conducted in vitro with a commonly used cell line model of EVT, HTR8/SVneo.
  
  
  CONCLUSIONS
  This study is the first to comprehensively examine the effects of the eotaxin group of chemokines on EVT functions and demonstrates that all the three eotaxins have the ability to regulate EVT functions critical to their role in vessel remodelling. This identifies a new role for the eotaxin group of chemokines during placentation.
authors.affiliation: Department of Perinatal Medicine, Pregnancy Research Centre and University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Locked Bag 300, Corner Grattan Street and Flemington Road, Parkville, VIC 3052, Australia.
authors.forename: Simon E
authors.initials: SE
authors.lastname: Chau
authors.forename: Padma
authors.initials: P
authors.lastname: Murthi
authors.forename: May H
authors.initials: MH
authors.lastname: Wong
authors.forename: Guy StJ
authors.initials: GS
authors.lastname: Whitley
authors.forename: Shaun P
authors.initials: SP
authors.lastname: Brennecke
authors.forename: Rosemary J
authors.initials: RJ
authors.lastname: Keogh
mesh_terms: D001160:Arterioles / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D054413:Chemokine CCL11 / Q000494:pharmacology* / Q000502:physiology; D054423:Chemokine CCL24 / Q000494:pharmacology* / Q000502:physiology; D000075906:Chemokine CCL26; D019742:Chemokines, CC / Q000494:pharmacology* / Q000502:physiology; D003094:Collagen; D003656:Decidua / Q000098:blood supply / Q000187:drug effects*; D004338:Drug Combinations; D005260:Female; D006801:Humans; D007797:Laminin; D011509:Proteoglycans; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects*
chemical_list: C515456:CCL11 protein, human; C515484:CCL24 protein, human; C120429:CCL26 protein, human; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D000075906:Chemokine CCL26; D019742:Chemokines, CC; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen
keywords: adhesion; chemokine; eotaxins; extravillous trophoblast; invasion