title: Synergistic effect of methionine encephalin (MENK) combined with pidotimod(PTD) on the maturation of murine dendritic cells (DCs).
abstract: To gain new insight into the functional interaction between dendritic cells and methionine encephalin (MENK) combined with pidotimod (PTD), we have analyzed the effect of MENK plus PTD on the morphology, phenotype and functions of murine bone-marrow derived dendritic cells (BMDCs) in vitro. The maturation of BMDCs cultured in the presence of either MENK or PTD alone, or MENK in combination with PTD, was detected. The cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt/phenazinemethosulphate (MTS/PMS). The changes of BMDCs morphology were confirmed with light microscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The BMDCs treated with MENK combined with PTD displayed a higher expression of typical maturation markers of CD40, CD80, CD83, CD86 and MHC-IIidentified by fluorescence activated cell sorting (FACS), and stronger ability to drive T cells. The decrease of the endocytic ability was assayed by DAB kit, FITC-dextran and cellular immunohistochemistry. Finally upregulation of cytokines production of IL-12 and TNF-α was determined by ELISA. These data indicate that MENK combined with PTD could exert synergistic action on BMDC maturation.
authors.affiliation: Department of immunology; School of Basic Medical Science; China Medical University; Shenyang, P.R. China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Wang
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Zhang
authors.forename: Enhua
authors.initials: E
authors.lastname: Wang
authors.forename: Nicollas P
authors.initials: NP
authors.lastname: Plotnikoff
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D004705:Endocytosis; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007155:Immunologic Factors / Q000494:pharmacology*; D018664:Interleukin-12 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008853:Microscopy; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D053778:Thiazolidines / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; D007155:Immunologic Factors; D053778:Thiazolidines; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D004744:Enkephalin, Methionine; C064893:pidotimod; D011761:Pyrrolidonecarboxylic Acid
keywords: BMDCs; MENK; PTD; maturation; synergistic effect