title: Line temporal focusing characteristics in transparent and scattering media.
abstract: Line illumination geometries have advantageous properties for temporal focusing nonlinear microscopy. The characteristics of line temporal focusing (LITEF) in transparent and scattering media are studied here both experimentally and using numerical model simulations. We introduce an approximate analytical formula for the dependence of axial sectioning on the laser and microscope's parameters. Furthermore, we show that LITEF is more robust to tissue scattering than wide-field temporal focusing, and can penetrate much deeper into scattering tissue while maintaining good sectioning capabilities. Based on these observations, we propose a new design for LITEF-based tissue imaging at depths that could potentially exceed the out-of-focus physical excitation limit.
authors.affiliation: Faculty of Biomedical Engineering, The Technion – IIT, Haifa, Israel.
authors.forename: Hod
authors.initials: H
authors.lastname: Dana
authors.forename: Nimrod
authors.initials: N
authors.lastname: Kruger
authors.forename: Aviv
authors.initials: A
authors.lastname: Ellman
authors.forename: Shy
authors.initials: S
authors.lastname: Shoham
mesh_terms: D000818:Animals; D003198:Computer Simulation; D006801:Humans; D008962:Models, Theoretical; D055620:Optical Phenomena; D012542:Scattering, Radiation