title: [Evaluation of biocompatibility of modified gelatin composite membranes for corneal regeneration].
abstract: In order to investigate the feasibility of the modified chitosan-gelatin crosslinked membrane (MC-Gel) and chitosan-gelatin crosslinked membrane (CS-Gel) to be a potential biomaterial for corneal regeneration, we evaluated their physicochemical properties and intraocular biocompatibility in this study. White light transmission and permeability of these membranes were detected. Results showed that white light transmission of both membranes was above 90% at 500 nm, which was similar to that of human cornea. The glucose, tryptophan and NaCl permeability of MC-Gel membrane and CS-Gel membrane was better than or similar to those of human cornea. The methylthiazol tetrazolium (MTT) assay was used to assess cell viability and proliferation. Also, interlamellar corneal transplantation was carried out to evaluate ophthalmic biocompatibility of MC-Gel membrane and CS-Gel membrane. Results indicated that MC-Gel membranes could support the proliferation of HCEC and displayed good intraocular biocompatibility when implanted into rabbits. No severe inflammatory reaction occurred after transplantation and the implanted MC-Gel membrane degraded completely 16 weeks post-operation. Due to its good physicochemical properties and biocompatibility, MC-Gel membrane could be a promising candidate material for corneal regeneration.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Long
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.forename: Jiaming
authors.initials: J
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Binqian
authors.initials: B
authors.lastname: Liu
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003315:Cornea / Q000166:cytology; D065306:Corneal Injuries; D003432:Cross-Linking Reagents; D019573:Epithelium, Corneal / Q000166:cytology* / Q000502:physiology / Q000601:surgery; D005780:Gelatin / Q000737:chemistry*; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D011817:Rabbits; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D005780:Gelatin; D048271:Chitosan