title: Molecular cloning, characterization and immunological response analysis of Toll-like receptor 21 (TLR21) gene in grass carp, Ctenopharyngodon idella.
abstract: TLR21, a non-mammalian Toll-like receptor, has been recently identified in fishes, frogs and birds. In the present study, the full-length cDNA sequence of TLR21 (CiTLR21) from Ctenopharyngodon idella has been isolated and characterized. The CiTLR21 full-length cDNA sequence consists of 3578bp, with an open reading frame (ORF) of 2958bp encoding 985 amino acid residues. The putative CiTLR21 protein contains a signal peptide sequence, 17 leucine-rich (LRR) motifs, a transmembrane region and a Toll/interleukin-1 receptor (TIR) domain. The CiTLR21 gene is expressed in a wide range of tissues with the highest expression in skin. Upon induction by Aquareovirus, CiTLR21 expression is significantly down-regulated in liver and spleen, whereas is significantly up-regulated in liver and spleen after Aeromonas hydrophila infection. These results suggest that CiTLR21 plays an important role in Aquareovirus and A. hydrophila-related diseases. This work may provide the basis for further investigations into the immune system of grass carp and other teleost fishes.
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wang
authors.forename: Yubang
authors.initials: Y
authors.lastname: Shen
authors.forename: Narayan Prasad
authors.initials: NP
authors.lastname: Pandit
authors.forename: Jiale
authors.initials: J
authors.lastname: Li
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D002347:Carps / Q000235:genetics / Q000276:immunology* / Q000821:virology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D007113:Immunity, Innate; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000821:virology; D009928:Organ Specificity; D010802:Phylogeny; D012087:Reoviridae / Q000276:immunology; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000821:virology; D051193:Toll-Like Receptors / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D051193:Toll-Like Receptors