title: In vitro susceptibility of Mycoplasma hyopneumoniae field isolates and occurrence of fluoroquinolone, macrolides and lincomycin resistance.
abstract: A total of 159 Thai isolates of Mycoplasma hyopneumoniae isolated from pneumonic lungs of pigs during 2006-2011 were investigated for their in vitro susceptibility to 12 antimicrobial agents. Low activity of chlortetracycline was indicated by the MIC range from 3.12-100 μg/ml and MIC90 of 50 μg/ml. Seventy-six isolates showed resistance to enrofloxacin, whereas 2 isolates showed resistance to macrolides and lincomycin. In addition, a point mutation at A2058G was revealed by sequence analysis of 23S ribosomal RNA in both isolates. The present results confirmed the rapid increase of resistant M. hyopneumoniae isolates against chlortetracycline, enrofloxacin, macrolides and lincomycin in Thailand. Selection of drugs to control swine diseases in Thailand must be done more prudently in consideration of reducing the antimicrobial resistance.
authors.affiliation: National Institute of Animal Health, Bangkok 10900, Thailand.
authors.forename: Pacharee
authors.initials: P
authors.lastname: Thongkamkoon
authors.forename: Watcharachai
authors.initials: W
authors.lastname: Narongsak
authors.forename: Hideki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Pornpen
authors.initials: P
authors.lastname: Pathanasophon
authors.forename: Masato
authors.initials: M
authors.lastname: Kishima
authors.forename: Koshi
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D026141:Drug Resistance, Multiple, Fungal; D024841:Fluoroquinolones; D008034:Lincomycin; D008168:Lung / Q000382:microbiology*; D018942:Macrolides; D045705:Mycoplasma hyopneumoniae / Q000187:drug effects*; D045729:Pneumonia of Swine, Mycoplasmal / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013552:Swine; D013785:Thailand
chemical_list: D000935:Antifungal Agents; D017931:DNA Primers; D024841:Fluoroquinolones; D018942:Macrolides; D008034:Lincomycin