title: High-nitrate wastewater treatment in an expanded granular sludge bed reactor and microbial diversity using 454 pyrosequencing analysis.
abstract: Denitrification of high concentration of nitrate wastewater was investigated in expanded granular sludge bed (EGSB) reactor with sodium acetate as the carbon source. The optimal parameters were achieved with C/N mole ratio of 2.0, liquid up-flow velocity (Vup) of 3.0 m/h and pH of 6.2-8.2. Complete denitrification can be achieved even with nitrate nitrogen concentration as high as 14000 mg/L. Furthermore, 454-pyrosequencing technology was used to analyze bacterial diversity. Results showed that a total of 5573 sequences were obtained which could be affiliated to 6 phylogenetic groups, including Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Chloroflexi and unclassified phylum. Proteobacteria (84.53%) was the dominant microbial population, followed by Firmicutes (13.24%) and Actinobacteria (0.38%). The dominate phylum was different from that in other anaerobic system.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Runhua
authors.initials: R
authors.lastname: Liao
authors.forename: Ke
authors.initials: K
authors.lastname: Shen
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Shi
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000032:analysis; D004275:DNA, Ribosomal / Q000235:genetics; D058440:Denitrification; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000032:analysis* / Q000302:isolation & purification; D009573:Nitrites / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D012722:Sewage / Q000382:microbiology*; D013696:Temperature; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology; D018508:Water Purification / Q000295:instrumentation*
chemical_list: D002244:Carbon; D004275:DNA, Ribosomal; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater