title: A deep-blue OLED-based biochip for protein microarray fluorescence detection.
abstract: Integrated biochips exploit a multi-disciplinary approach to produce portable point-of-care medical diagnostic systems that uncouple diagnosis from centralized laboratories. These portable devices are cost effective and have several advantages including broader accessibility to health care worldwide. Fluorescence detection of a disease-specific probe excited by an optical source is one of the most diffused methods for quantitative analysis on biochips. Here we designed and characterized a miniaturized biochip based on a novel deep-blue organic light-emitting diode. The molecular design of the diode was optimized to excite a fluorophore-conjugated antibody and tested on a protein microarray configuration with good sensitivity and specificity. These findings will be instrumental for the development of next generation point-of-care biochips.
authors.affiliation: Laboratory of Virology, the International Centre for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, 34149 Trieste, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Marcello
authors.forename: Daniele
authors.initials: D
authors.lastname: Sblattero
authors.forename: Cristina
authors.initials: C
authors.lastname: Cioarec
authors.forename: Paolo
authors.initials: P
authors.lastname: Maiuri
authors.forename: Patrizia
authors.initials: P
authors.lastname: Melpignano
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis; D019204:GTP-Binding Proteins / Q000032:analysis; D006801:Humans; D018796:Immunoconjugates / Q000032:analysis; D008027:Light; D019095:Point-of-Care Systems; D040081:Protein Array Analysis / Q000295:instrumentation*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D012680:Sensitivity and Specificity; D011503:Transglutaminases / Q000032:analysis
chemical_list: D005456:Fluorescent Dyes; D018796:Immunoconjugates; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins