title: Secretory vesicle analysis for discovery of low abundance plasma biomarkers.
abstract: BACKGROUND
  Pathological conditions can be reflected, as well as propagated, by changes in the expression patterns of secreted proteins. Protein secretion may occur by signal sequence dependent or independent mechanisms and many secreted proteins with desirable biomarker characteristics appear to be low abundance proteins in body fluids. Both factors complicate disease-associated secreted protein discovery.
  
  
  OBJECTIVE
  To enhance the discovery of low abundance, physiologically relevant, plasma protein biomarkers.
  
  
  METHODS
  Biomarker discovery has been performed in media, body fluids or tissue homogenates. A comparative analysis of the contents of secretory vesicles isolated directly from affected tissues or model systems substantially improves the detection of relevant, low abundance plasma biomarkers.
  
  
  RESULTS
  Evidence supporting this approach is provided from pilot experiments in prostate cancer.
authors.affiliation: Group Leader & Director Caprion Proteomics, Inc., Department of Cell and Molecular Biology, 7150 Alexander-Fleming Montreal, Quebec, H4S 2C8 Canada +1 514 228 3627 ; +1 514 940 3619 ; eparamithiotis@caprion.com.
authors.forename: Joel
authors.initials: J
authors.lastname: Lanoix
authors.forename: Eustache
authors.initials: E
authors.lastname: Paramithiotis