title: Potential of capillary electrophoresis (CE) and chip-CE with dual detection (capacitively-coupled contactless conductivity detection (C4D) and fluorescence detection) for monitoring of nicotine and cotinine derivatization.
abstract: Capillary electrophoresis (CE) coupled with capacitively-coupled contactless conductivity (C(4)D) and fluorescence (FD) detectors and chip-CE for monitoring of nicotine and cotinine derivatization was demonstrated. Separation of the substrates and intermediates could be achieved by CE-C(4)D in 7 min (R(s) > 2.7) using 45 mM acetic acid (pH 3.0) and this system was applied to detect the intermediate formation. Final fluorescent products could be analyzed by micellar electrokinetic chromatography (MEKC-FD) in 5 mM borate buffer (pH 9.0) containing 10 mM sodium dodecylsulfate (SDS) (%RSD < 3.00%). Transferring of MEKC-FD to chip-CE allowed for shorter analysis time (2.5 min) and decreased sample consumption. The chip-CE-FD shows good detection and quantitation limits (< 7.5 µM) and precision (%RSD < 2.81%) and was employed to determine nicotine and cotinine in artificial urine. This work reveals the potential of CE and chip-CE with dual detectors as simultaneous, convenient and rapid methods for monitoring pyridine derivatization.
authors.affiliation: Faculty of Pharmacy, Siam University, Bangkok, Thailand.
authors.forename: Nantana
authors.initials: N
authors.lastname: Nuchtavorn
authors.forename: Marketa
authors.initials: M
authors.lastname: Ryvolova
authors.forename: Fritz
authors.initials: F
authors.lastname: Bek
authors.forename: Mirek
authors.initials: M
authors.lastname: Macka
authors.forename: Chutima
authors.initials: C
authors.lastname: Phechkrajang
authors.forename: Leena
authors.initials: L
authors.lastname: Suntornsuk
mesh_terms: D003367:Cotinine / Q000032:analysis* / Q000737:chemistry*; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009538:Nicotine / Q000032:analysis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D009538:Nicotine; D003367:Cotinine