title: [Effects of magnetic c-erbB-2 antisense probe of different concentrations on morphology and expression of SK-Br-3 cancer cell lines in vitro].
abstract: OBJECTIVE
  To explore the effects of the magnetic c-erbB-2 antisense probe of different concentrations on the morphology and expression of SK-Br-3 cancer cells in vitro.
  
  
  METHODS
  Breast cancer SK-Br-3 cells were transfected for 24 h by antisense probe at an iron concentration of 5, 10, 25, 50, and 100 mg/L, respectively. The distribution and content of iron particles in SK-Br-3 cells was determined by Prussian blue staining, electron microscopy, and atomic absorption spectrometry. Cell viability was observed by trypan-blue exclusion and CCK-8 test. The protein expression of c-erbB-2 was assessed by the Western blot analysis. The changes of the signal strength were considered by magnetic resonance imaging (MRI).
  
  
  RESULTS
  c-erbB-2 antisense probe was uptake by SK-Br-3 cells in a concentration-dependence manner within a certain range (5, 10, and the Medicine Scientific Research Project of Chongqing Health Bureau (062025)25 mg/L). When the probe concentration was 25 mg/L, iron content in cells was (18.38±0.28) pg, the cell vitality, survival, and c-erbB-2 protein expression were reduced significantly (all P<0.05), and the T2 value was lower significantly (P<0.05). However, the results of 50 mg/L or 100 mg/L group showed no significant difference with the 25 mg/L group (P>0.05).
  
  
  CONCLUSIONS
  The magnetic c-erbB-2 antisense probe can effectively transfect and specifically inhibit the expression of SK-Br-3 cell lines at the iron concentration of 25 mg/L.
authors.affiliation: Department of Radiology, Chongqing Medical University, Chongqing, China.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Liu
authors.forename: Zhi-peng
authors.initials: ZP
authors.lastname: Wen
authors.forename: Ming
authors.initials: M
authors.lastname: Wen
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: He
authors.forename: Shu-de
authors.initials: SD
authors.lastname: Tan
authors.forename: Shao-lin
authors.initials: SL
authors.lastname: Li
mesh_terms: D016375:Antisense Elements (Genetics) / Q000235:genetics*; D017209:Apoptosis; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008280:Magnetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D016375:Antisense Elements (Genetics); D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human