title: Core-shell Fe3O4-Au magnetic nanoparticles based nonenzymatic ultrasensitive electrochemiluminescence immunosensor using quantum dots functionalized graphene sheet as labels.
abstract: In this paper, a novel, low-cost electrochemiluminescence (ECL) immunosensor using core-shell Fe3O4-Au magnetic nanoparticles (AuMNPs) as the carriers of the primary antibody of carbohydrate antigen 125 (CA125) was designed. Graphene sheet (GS) with property of good conductivity and large surface area was a captivating candidate to amplify ECL signal. We successively synthesized functionalized GS by loading large amounts of quantum dots (QDs) onto the poly (diallyldimethyl-ammonium chloride) (PDDA) coated graphene sheet (P-GS@QDs) via self-assembly electrostatic reactions, which were used to label secondary antibodies. The ECL immunosensors coupled with a microfluidic strategy exhibited a wide detection range (0.005-50 U mL(-1)) and a low detection limit (1.2 mU mL(-1)) with the help of an external magnetic field to gather immunosensors. The method was evaluated with clinical serum sample, receiving good correlation with results from commercially available analytical procedure.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
authors.forename: Jiadong
authors.initials: J
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
mesh_terms: D018394:CA-125 Antigen / Q000097:blood*; D005290:Ferric Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D008163:Luminescent Measurements; D008280:Magnetics; D008565:Membrane Proteins / Q000097:blood*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000737:chemistry; D045663:Quantum Dots; D015203:Reproducibility of Results; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D018394:CA-125 Antigen; D005290:Ferric Compounds; C451759:MUC16 protein, human; D008565:Membrane Proteins; D014867:Water; C000499:ferric oxide; D006046:Gold; D006108:Graphite