title: Extended lifespan of normal human B lymphocytes experimentally infected by SV40 or transfected by SV40 large T antigen expression vector.
abstract: SV40 footprints were detected in different lymphoproliferative disorders and in blood specimens of healthy donors. However, little is known on the ability of SV40 to infect/transform normal human B-lymphocytes. In this in vitro study, experimental SV40 infection and SV40 Tag transfection of normal human B-lymphocytes from healthy blood donors were carried out. In SV40 infected/transfected purified B-cells, during the time course analyses, viral DNA sequences were detected by PCR, while Tag mRNA and protein were revealed by RT-PCR and immunocytochemistry, respectively. Trypan blue and Alamar blue assays showed an increase in number of cells and cell viability of infected/transfected B-cells up to day 50, then a drastic and constant cell number reduction was observed in cultures. Approximately 50% of both infected and transfected B-cells appeared morphologically transformed. SV40 viral progeny and its titer from infected B-cells was determined by plaque assay in permissive CV-1 cells. Our data indicate that human B-cells can be efficiently infected by SV40, release a viral progeny, while at the same time are transformed. SV40 infected/Tag transfected B-cells may represent an experimental model of study for investigating new biomarkers and targets for innovative therapeutic approaches in human B-cell malignancies.
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Cell Biology and Molecular Genetics, University of Ferrara, Ferrara, Italy.
authors.forename: Franca Nneka
authors.initials: FN
authors.lastname: Alaribe
authors.forename: Elisa
authors.initials: E
authors.lastname: Mazzoni
authors.forename: Gian Matteo
authors.initials: GM
authors.lastname: Rigolin
authors.forename: Lara
authors.initials: L
authors.lastname: Rizzotto
authors.forename: Stefania
authors.initials: S
authors.lastname: Maniero
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pancaldi
authors.forename: Marco
authors.initials: M
authors.lastname: Manfrini
authors.forename: Fernanda
authors.initials: F
authors.lastname: Martini
authors.forename: Mauro G
authors.initials: MG
authors.lastname: Tognon
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000502:physiology; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000502:physiology* / Q000821:virology; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D027601:Polyomavirus Infections / Q000473:pathology; D013539:Simian virus 40 / Q000502:physiology*; D013997:Time Factors; D014162:Transfection; D014412:Tumor Virus Infections / Q000473:pathology
chemical_list: D000952:Antigens, Polyomavirus Transforming