title: Processing of fluorescence lifetime image using modified phasor approach: homo-FRET from the acceptor.
abstract: As the hardware of FLIM technique becomes mature, the most important criterion for FLIM application is the correct interpretation of its data. In this research, first of all, a more orthogonal phasor approach, called as Modified Phasor Approach (MPA), is put forward. It is a way to calculate the lifetime of the complex fluorescent process, and a rule to measure how much the fluorescence process deviates from single exponential decay. Secondly, MPA is used to analysis the time-resolved fluorescence processes of the transfected CHO-K1 Cell lines expressing adenosine receptor A1R tagged by CYP and YFP, measured in the channel of the acceptor. The image of the fluorescence lifetime and the multiplication of the fluorescence lifetime and deviation from single exponential decay reveal the details of the Homo-FRET. In one word, MPA provides the physical meaning in its whole modified phasor space, and broadens the way for the application of the fluorescence lifetime imaging.
authors.affiliation: Faculty of Automation, Guangdong University of Technology, Guangzhou, Guangdong, 510006, People's Republic of China. zhouyanzhou.optics@gmail.com
authors.forename: Yanzhou
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yulei
authors.initials: Y
authors.lastname: Bai
authors.forename: Ci
authors.initials: C
authors.lastname: Chen
authors.forename: John M
authors.initials: JM
authors.lastname: Dickenson
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D013997:Time Factors