title: Isolation and establishment of hair follicle dermal papilla cell cultures.
abstract: The isolation of hair follicle dermal papilla cells has become an important technique in the field of cutaneous stem cell biology. These cells can be used for a number of biological and translational purposes. They are studied to identify the cellular characteristics and molecular factors that underpin the initiation, maintenance, and modulation of hair growth; to develop new human hair replacement techniques; and as a source of cells capable of being directed down a variety of different lineages. Here, we describe the isolation of hair follicle dermal papilla cells from both human and murine sources via the microdissection techniques used in our lab.
authors.affiliation: School of Biological and Biomedical Sciences, Durham University, Durham, UK.
authors.forename: Karl
authors.initials: K
authors.lastname: Gledhill
authors.forename: Aaron
authors.initials: A
authors.lastname: Gardner
authors.forename: Colin A B
authors.initials: CA
authors.lastname: Jahoda
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D051379:Mice; D013234:Stem Cells / Q000166:cytology