title: PKCδ enhances C/EBPα degradation via inducing its phosphorylation and cytoplasmic translocation.
abstract: Our previous study has shown that PKCδ stimulates proteasome-dependent degradation of C/EBPα, which partially contributes to PKCδ-mediated apoptosis. However, the molecular interrelationship between these two important proteins is still unknown. In this study, we reported that C/EBPα was phosphorylated by activated PKCδ on three serines, two of which were reported for the first time. Phosphorylated C/EBPα underwent cytoplasmic translocation, which led to the inactivation of its transcriptional activity. Inactive cytoplasmic C/EBPα was finally subjected to proteasome degradation. This work reveals the exquisite molecular events linking activated PKCδ and C/EBPα degradation during cell apoptosis.
authors.affiliation: The Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Wu
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Xia
authors.forename: Yun
authors.initials: Y
authors.lastname: Yu
authors.forename: Shao-Ming
authors.initials: SM
authors.lastname: Shen
authors.forename: San-Feng
authors.initials: SF
authors.lastname: Han
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Tong-Dan
authors.initials: TD
authors.lastname: Wang
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
authors.forename: Li-Shun
authors.initials: LS
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000502:physiology; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D051745:Protein Kinase C-delta / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012694:Serine / Q000378:metabolism
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D012694:Serine; D051745:Protein Kinase C-delta