title: Effect of sinapic acid against carbon tetrachloride-induced acute hepatic injury in rats.
abstract: Acute hepatic inflammation is regarded as a hallmark of early stage fibrosis, which can progress to extensive fibrosis and cirrhosis. Sinapic acid is a phenylpropanoid compound that is abundant in cereals, nuts, oil seeds, and berries and has been reported to exhibit a wide range of pharmacological properties. In this study, we investigated the anti-inflammatory effect of sinapic acid in carbon tetrachloride (CCl4)-induced acute hepatic injury in rats. Sinapic acid was administered orally (10 or 20 mg/kg) to rats at 30 min and 16 h before CCl4 intoxication. Sinapic acid treatment of rats reduced CCl4-induced abnormalities in liver histology, serum alanine transaminase and aspartate transaminase activities, and liver malondialdehyde levels. In addition, sinapic acid treatment significantly attenuated the CCl4-induced production of inflammatory mediators, including tumor necrosis factor-alpha and interleukin-1β mRNA levels, and increased the expression of nuclear factor-kappa B (NF-κB p65). Sinapic acid exhibited strong free radical scavenging activity in vitro. Thus, sinapic acid protected the rat liver from CCl4-induced inflammation, most likely by acting as a free radical scavenger and modulator of NF-κB p65 activation and proinflammatory cytokine expression. Sinapic acid may thus have potential as a therapeutic agent for suppressing hepatic inflammation.
authors.affiliation: College of Pharmacy, Pusan National University, Busan, 609-735, Korea.
authors.forename: Dong-Su
authors.initials: DS
authors.lastname: Shin
authors.forename: Kung Wook
authors.initials: KW
authors.lastname: Kim
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
authors.forename: Jeon-Ok
authors.initials: JO
authors.lastname: Moon
mesh_terms: D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000737:chemistry; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D003373:Coumaric Acids / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D015394:Molecular Structure; D009929:Organ Size / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D001713:Biphenyl Compounds; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D053583:Interleukin-1beta; D010851:Picrates; D051996:Transcription Factor RelA; D053773:Transforming Growth Factor beta1; C073734:sinapinic acid; D002251:Carbon Tetrachloride; C004931:1,1-diphenyl-2-picrylhydrazyl