title: Dihydroartemisinin induces radiosensitivity in cervical cancer cells by modulating cell cycle progression.
abstract: OBJECTIVE
  To investigate the radiosensitizing effects of dihydroartemisinin (DHA) and its underlying mechanisms in cervical cancer cells.
  
  
  METHODS
  This experimental study was conducted between May 2009 and August 2012 in the School of Radiation Medicine and Protection, Soochow University, Suzhou, China. HeLa and Siha cells were assigned as the control group and DHA as treated group. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, clonogenic assay, cell cycle analysis, and apoptosis analysis were carried out in 2 cell lines of both groups.
  
  
  RESULTS
  The inhibitory effect of DHA on the HeLa and Siha cell lines was dependent on both concentration and time. Dihydroartemisinin increased the radiosensitivity of HeLa cells, but not of Siha cells. Apoptosis and the gap2/mitosis (G2/M) phase transition induced by x-irradiation was enhanced by DHA treatment in HeLa cells. Irradiation, combined with DHA, decreased Wee1 expression while increasing Cyclin B1 expression in HeLa cells.
  
  
  CONCLUSIONS
  Dihydroartemisinin potently abrogates G2 checkpoint control in HeLa cells. It can relieve the G2/M arrest induced by irradiation; thus, it can be used as an effective radiosensitizer, which will probably promote the entry of more irradiation-damaged cells into mitosis.
authors.affiliation: Department of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
authors.forename: Judong
authors.initials: J
authors.lastname: Luo
authors.forename: Xialin
authors.initials: X
authors.lastname: Chen
authors.forename: Guanglie
authors.initials: G
authors.lastname: Chen
authors.forename: Xifa
authors.initials: X
authors.lastname: Zhou
authors.forename: Xujing
authors.initials: X
authors.lastname: Lu
authors.forename: Yang
authors.initials: Y
authors.lastname: Ling
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Jianping
authors.initials: J
authors.lastname: Cao
mesh_terms: D037621:Artemisinins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D005260:Female; D016195:G2 Phase / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D011836:Radiation Tolerance / Q000187:drug effects*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
chemical_list: D037621:Artemisinins; C039060:artenimol