title: Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar.
abstract: Phytophthora root rot (PRR), caused by Phytophthora sojae Kaufmann & Gerdemann, is one of the most destructive diseases of soybean [Glycine max (L.) Merr.]. Deployment of resistance genes is the most economical and effective way of controlling the disease. The soybean cultivar 'Yudou 29' is resistant to many P. sojae isolates in China. The genetic basis of the resistance in 'Yudou 29' was elucidated through an inheritance study and molecular mapping. In response to 25 P. sojae isolates, 'Yudou 29' displayed a new resistance reaction pattern distinct from those of differentials carrying known Rps genes. A population of 214 F2:3 families from a cross between 'Jikedou 2' (PRR susceptible) and 'Yudou 29' was used for Rps gene mapping. The segregation fit a ratio of 1:2:1 for resistance:segregation:susceptibility within this population, indicating that resistance in 'Yudou 29' is controlled by a single dominant gene. This gene was temporarily named RpsYD29 and mapped on soybean chromosome 03 (molecular linkage group N; MLG N) flanked by SSR markers SattWM82-50 and Satt1k4b at a genetic distance of 0.5 and 0.2 cM, respectively. Two nucleotide binding site-leucine rich repeat (NBS-LRR) type genes were detected in the 204.8 kb region between SattWM82-50 and Satt1k4b. These two genes showed high similarity to Rps1k in amino acid sequence and could be candidate genes for PRR resistance. Based on the phenotype reactions and the physical position on soybean chromosome 03, RpsYD29 might be a novel allele at, or a novel gene tightly linked to, the Rps1 locus.
authors.affiliation: MOA Key Lab of Soybean Biology (Beijing), the National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, People's Republic of China.
authors.forename: Jiqing
authors.initials: J
authors.lastname: Zhang
authors.forename: Changjian
authors.initials: C
authors.lastname: Xia
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.forename: Canxing
authors.initials: C
authors.lastname: Duan
authors.forename: Suli
authors.initials: S
authors.lastname: Sun
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wu
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D002874:Chromosome Mapping; D016000:Cluster Analysis; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D010838:Phytophthora / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013025:Glycine max / Q000235:genetics* / Q000382:microbiology; D014774:Virulence