title: Lectin glycoprofiling of recombinant therapeutic interleukin-7.
abstract: Lectins array is a powerfull and complementary method of glycans analysis allowing fast identification of specific motifs on molecules or cells. This technology is of increased interest for the development of therapeutic recombinant glycoproteins and particularly relevant for a first study of lot-to-lot comparison, or detection of unwanted glycans. In this chapter, we describe a lectin array-type method specifically designed for the study of recombinant therapeutic interleukin-7 (rhIL-7). This specific method allows the analysis of the glycans motifs, the distribution of the glycoforms population, and the detection of potential immunogen glycans in rhIL-7 purified CHO-produced batches.
authors.affiliation: GLYcoDiag, Orléans, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Landemarre
authors.forename: Eric
authors.initials: E
authors.lastname: Duverger
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D006023:Glycoproteins / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D015851:Interleukin-7 / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D009439:Neuraminidase / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D006023:Glycoproteins; C508605:IL7 protein, human; D015851:Interleukin-7; D037102:Lectins; D011134:Polysaccharides; D011994:Recombinant Proteins; D009439:Neuraminidase