title: High-resolution metabolic mapping of cell types in plant roots.
abstract: Metabolite composition offers a powerful tool for understanding gene function and regulatory processes. However, metabolomics studies on multicellular organisms have thus far been performed primarily on whole organisms, organs, or cell lines, losing information about individual cell types within a tissue. With the goal of profiling metabolite content in different cell populations within an organ, we used FACS to dissect GFP-marked cells from Arabidopsis roots for metabolomics analysis. Here, we present the metabolic profiles obtained from five GFP-tagged lines representing core cell types in the root. Fifty metabolites were putatively identified, with the most prominent groups being glucosinolates, phenylpropanoids, and dipeptides, the latter of which is not yet explored in roots. The mRNA expression of enzymes or regulators in the corresponding biosynthetic pathways was compared with the relative metabolite abundance. Positive correlations suggest that the rate-limiting steps in biosynthesis of glucosinolates in the root are oxidative modifications of side chains. The current study presents a work flow for metabolomics analyses of cell-type populations.
authors.affiliation: Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Arieh
authors.initials: A
authors.lastname: Moussaieff
authors.forename: Ilana
authors.initials: I
authors.lastname: Rogachev
authors.forename: Leonid
authors.initials: L
authors.lastname: Brodsky
authors.forename: Sergey
authors.initials: S
authors.lastname: Malitsky
authors.forename: Ted W
authors.initials: TW
authors.lastname: Toal
authors.forename: Heather
authors.initials: H
authors.lastname: Belcher
authors.forename: Merav
authors.initials: M
authors.lastname: Yativ
authors.forename: Siobhan M
authors.initials: SM
authors.lastname: Brady
authors.forename: Philip N
authors.initials: PN
authors.lastname: Benfey
authors.forename: Asaph
authors.initials: A
authors.lastname: Aharoni
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D055442:Metabolome / Q000502:physiology*; D018517:Plant Roots / Q000166:cytology* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant