title: Feeding strategies for enhanced lactobionic acid production from whey by Pseudomonas taetrolens.
abstract: High-level production of lactobionic acid from whey by Pseudomonas taetrolens under fed-batch fermentation was achieved in this study. Different feeding strategies were evaluated according to the physiological status and fermentation performance of P. taetrolens. A lactobionic acid titer of 164 g/L was obtained under co-feeding conditions affording specific and volumetric productivities of 1.4 g/g h and 2.05 g/L h, respectively. Flow cytometry assessment revealed that P. taetrolens cells exhibited a robust physiological status, which makes them particularly well-suited for employing concentrated nutrient solutions to further prolong the growth and production phases. Such detailed knowledge of the physiological status has been revealed to be a key issue to further support the development of high-yield lactobionic acid production processes under feeding strategies. The present study has demonstrated the feasibility of P. taetrolens to achieve high-level bio-production of lactobionic acid from whey through fed-batch cultivation, suggesting its major potential for industrial-scale implementation.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, Faculty of Chemistry, C/Julián Clavería s/n, 33071 Oviedo, Spain.
authors.forename: Saúl
authors.initials: S
authors.lastname: Alonso
authors.forename: Manuel
authors.initials: M
authors.lastname: Rendueles
authors.forename: Mario
authors.initials: M
authors.lastname: Díaz
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D003611:Dairy Products; D004187:Disaccharides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D011549:Pseudomonas / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004187:Disaccharides; C005608:lactobionic acid