title: Depolymerization of fucosylated chondroitin sulfate from sea cucumber, Pearsonothuria graeffei, via 60Co irradiation.
abstract: A method for depolymerization of a novel fucosylated chondroitin sulfate from Pearsonothuria graeffei (fCS-Pg) using (60)Co irradiation in water solution was developed in the current study. Fragments with varying molecular weights were obtained by (60)Co irradiation at different dosages and sample concentrations. The chemical compositions and structures of these fragments were further investigated using high-performance liquid chromatography (HPLC), infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR). Our results indicated that (60)Co irradiation induced depolymerization via selective breakage of glucuronic acid units in the fCS-Pg backbone, with no obvious influence on sulfated fucose branches under mild conditions. The recommended conditions for fCS-Pg degradation were 2-10% solution concentration and irradiation dosages of 10-50kGy. The anticoagulant activities of the low molecular weight fragments were additionally evaluated. Notably, anticoagulant activities were reduced with decreasing molecular weights. Compared to the native fCS-Pg, low molecular weight fragments displayed significantly decreased anticoagulant activities. Based on the collective findings, we propose that these fragments are potentially applicable as antithrombotic agents with reduced bleeding risk relative to native fCS-Pg.
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.
authors.forename: Nian
authors.initials: N
authors.lastname: Wu
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Ningbo
authors.initials: N
authors.lastname: Liao
authors.forename: Xinzi
authors.initials: X
authors.lastname: Yin
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Hu
authors.forename: Yujing
authors.initials: Y
authors.lastname: Sun
authors.forename: Donghong
authors.initials: D
authors.lastname: Liu
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D002809:Chondroitin Sulfates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003037:Cobalt Radioisotopes / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology; D005720:Gamma Rays; D020723:Glucuronic Acid / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008970:Molecular Weight; D058105:Polymerization / Q000528:radiation effects*; D012616:Sea Cucumbers / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000925:Anticoagulants; D003037:Cobalt Radioisotopes; D005343:Fibrinolytic Agents; C517150:fucosylated chondroitin sulfate; D020723:Glucuronic Acid; D002809:Chondroitin Sulfates