title: Surface conjugation of zwitterionic polymers to inhibit cell adhesion and protein adsorption.
abstract: Non-fouling surfaces that resist non-specific protein adsorption and cell adhesion are desired for many biomedical applications such as blood-contact devices and biosensors. Therefore, surface conjugation of anti-fouling molecules has been the focus of many studies. In this study, layer-by-layer polyelectrolyte deposition was applied to create an amine-rich platform for conjugation of zwitterionic polymers. A tri-layer polyelectrolyte (TLP) coating representing poly(ethylene imine) (PEI), poly(acrylic acid)-g-azide and PEI was deposited on various polymeric substrates via layer-by-layer deposition and then crosslinked via UV irradiation. Carboxyl-terminated poly(sulfobetaine methacrylate) p(SBMA) or poly(carboxybetaine methacrylate) p(CBMA) was then conjugated onto TLP coated substrates via a carbodiimide reaction. Our results demonstrate that the zwitterionic polymers could be easily conjugated over a wide pH range except under alkaline conditions, and almost completely block protein adsorption and the attachment of L929 cells and platelets. Therefore, this method has outstanding potential in biomedical applications that require low-fouling surfaces.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan.
authors.forename: Hsiu-Wen
authors.initials: HW
authors.lastname: Chien
authors.forename: Chih-Chi
authors.initials: CC
authors.lastname: Tsai
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.forename: Meng-Jiy
authors.initials: MJ
authors.lastname: Wang
authors.forename: Wei-Hsuan
authors.initials: WH
authors.lastname: Kuo
authors.forename: Ta-Chin
authors.initials: TC
authors.lastname: Wei
authors.forename: Sheng-Tung
authors.initials: ST
authors.lastname: Huang
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001622:Betaine / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology; D004573:Electrolytes / Q000737:chemistry; D005340:Fibrinogen / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005450:Fluorescamine; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007477:Ions; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D010973:Platelet Adhesiveness / Q000187:drug effects; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D004573:Electrolytes; D005353:Fibronectins; D007477:Ions; D008689:Methacrylates; D011109:Polymethacrylic Acids; C517178:polycarboxybetaine methacrylate; C521589:sulfobetaine methacrylate polymer; D014867:Water; D005450:Fluorescamine; D001622:Betaine; D005340:Fibrinogen