title: Identification of promoter motifs regulating ZmeIF4E expression level involved in maize rough dwarf disease resistance in maize (Zea Mays L.).
abstract: Maize rough dwarf disease (MRDD, a viral disease) results in significant grain yield losses, while genetic basis of which is largely unknown. Based on comparative genomics, eukaryotic translation initiation factor 4E (eIF4E) was considered as a candidate gene for MRDD resistance, validation of which will help to understand the possible genetic mechanism of this disease. ZmeIF4E (orthologs of eIF4E gene in maize) encodes a protein of 218 amino acids, harboring five exons and no variation in the cDNA sequence is identified between the resistant inbred line, X178 and susceptible one, Ye478. ZmeIF4E expression was different in the two lines plants treated with three plant hormones, ethylene, salicylic acid, and jasmonates at V3 developmental stage, suggesting that ZmeIF4E is more likely to be involved in the regulation of defense gene expression and induction of local and systemic resistance. Moreover, four cis-acting elements related to plant defense responses, including DOFCOREZM, EECCRCAH1, GT1GAMSCAM4, and GT1CONSENSUS were detected in ZmeIF4E promoter for harboring sequence variation in the two lines. Association analysis with 163 inbred lines revealed that one SNP in EECCRCAH1 is significantly associated with CSI of MRDD in two environments, which explained 3.33 and 9.04 % of phenotypic variation, respectively. Meanwhile, one SNP in GT-1 motif was found to affect MRDD resistance only in one of the two environments, which explained 5.17 % of phenotypic variation. Collectively, regulatory motifs respectively harboring the two significant SNPs in ZmeIF4E promoter could be involved in the defense process of maize after viral infection. These results contribute to understand maize defense mechanisms against maize rough dwarf virus.
authors.affiliation: National Key Facility of Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhong-guan-cun South St, 100081, Beijing, People's Republic of China. shiliyu321@yahoo.com.cn
authors.forename: Liyu
authors.initials: L
authors.lastname: Shi
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Weng
authors.forename: Changlin
authors.initials: C
authors.lastname: Liu
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Song
authors.forename: Hongqin
authors.initials: H
authors.lastname: Miao
authors.forename: Zhuanfang
authors.initials: Z
authors.lastname: Hao
authors.forename: Chuanxiao
authors.initials: C
authors.lastname: Xie
authors.forename: Mingshun
authors.initials: M
authors.lastname: Li
authors.forename: Degui
authors.initials: D
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Bai
authors.forename: Guangtang
authors.initials: G
authors.lastname: Pan
authors.forename: Xinhai
authors.initials: X
authors.lastname: Li
authors.forename: Shihuang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D060467:Disease Resistance / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012087:Reoviridae / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development / Q000821:virology
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D039561:Eukaryotic Initiation Factor-4E; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C011006:jasmonic acid; C036216:ethylene; D020156:Salicylic Acid