title: Resonance Raman scattering of catalytic beacons for DNA detection.
abstract: A novel method for analysing the catalytic action of a DNAzyme is reported. Resonance Raman scattering (RRS) is shown to successfully monitor the oxidation of two different peroxidase substrates and has been implemented in an assay for the detection of target DNA, providing a more sensitive method of analysis than current colorimetric techniques.
authors.affiliation: Centre for Molecular Nanometrology, West CHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral St., Glasgow, G1 1XL, UK.
authors.forename: Kristy S
authors.initials: KS
authors.lastname: McKeating
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
mesh_terms: D001560:Benzidines / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis*; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D001560:Benzidines; D052160:Benzothiazoles; D004269:DNA, Bacterial; D021881:DNA, Catalytic; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C021758:3,3',5,5'-tetramethylbenzidine; D010544:Peroxidases