title: Simultaneous in vivo phenotyping of CYP enzymes.
abstract: As major determinants of the duration of drug action the CYP enzymes strongly influence drug efficacy and toxicity. In vivo phenotyping for CYP activities using cocktails of well-tolerated CYP-specific substrates may be valuable in the development of personalized medicine protocols, particularly for drugs that have significant toxicity profiles. However, the use of the cocktail approach in the clinic is dependent on the rapid provision of patient-specific information to the clinician. Here we describe the application of liquid chromatography-tandem mass spectrometry (LC-MS-MS) for the simultaneous phenotyping of five major drug-metabolizing CYPs in patients within a 5-min assay.
authors.affiliation: Faculty of Pharmacy, University of Sydney, Sydney, NSW, Australia.
authors.forename: Sussan
authors.initials: S
authors.lastname: Ghassabian
authors.forename: Michael
authors.initials: M
authors.lastname: Murray
mesh_terms: D002110:Caffeine / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006801:Humans; D059625:Liquid-Liquid Extraction; D010641:Phenotype; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D013806:Theophylline / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002110:Caffeine; D003577:Cytochrome P-450 Enzyme System; D013806:Theophylline; C021183:1,7-dimethylxanthine