title: Evaluation of antimycobacterial activity of a sulphonamide derivative.
abstract: Mycobacterial infections including Mycobacterium tuberculosis have been increasing globally. The additional prevalence of multidrug-resistant (MDR-TB) strains and extensively drug-resistant tuberculosis (XDR-TB) stimulate an urgent need for the development of new drugs for the treatment of mycobacterial infections. It is very important to test the antimicrobial activity of novel compounds because they can be used in new with antimycobacterial drug formulation. Studies have shown that Mycobacterium smegmatis can be used in Minimum Inhibitory Concentration (MIC) assays with the advantage of rapidly and safely screen anti-tubercular compounds. This paper presents an evaluation of potential mycobacteriological compounds derived from inorganic synthesis and their microbiological performance along and in conjunction with Trimethoprim. Antimicrobial activity experiments were carried out by using the microdilution technique in broth to evaluate the sensibility against M. smegmatis. MIC values were between 0.153 and 4.88 μg/ml for the compounds tested. Tests of interaction between drugs were made by the method of Fractional Inhibitory Concentration Index (FICI). The compound [Au (sulfatiazolato)(PPh3)] showed synergism FICI = 0.037 and was evaluated by isobols.
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas - Universidade Federal de Santa Maria, Avenida Roraima 1000, 97105900 Santa Maria, RS, Brazil. vaagertt@gmail.com
authors.forename: Vanessa Albertina
authors.initials: VA
authors.lastname: Agertt
authors.forename: Lenice Lorenço
authors.initials: LL
authors.lastname: Marques
authors.forename: Pauline Cordenonsi
authors.initials: PC
authors.lastname: Bonez
authors.forename: Tanise Vendruscolo
authors.initials: TV
authors.lastname: Dalmolin
authors.forename: Gelson Noe
authors.initials: GN
authors.lastname: Manzoni de Oliveira
authors.forename: Marli Matiko Anraku
authors.initials: MM
authors.lastname: de Campos
mesh_terms: D000995:Antitubercular Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D008826:Microbial Sensitivity Tests / Q000379:methods; D020102:Mycobacterium smegmatis / Q000187:drug effects*; D013449:Sulfonamides / Q000008:administration & dosage / Q000494:pharmacology*; D014295:Trimethoprim / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000995:Antitubercular Agents; D013449:Sulfonamides; D014295:Trimethoprim