title: Characterization of Lactobacillus plantarum Lp27 isolated from Tibetan kefir grains: a potential probiotic bacterium with cholesterol-lowering effects.
abstract: Lactobacillus plantarum Lp27 was isolated from Tibetan kefir grains. The Lp27 isolate survived a 3-h incubation at pH 2.0 and grew normally in 0.3% oxgall. In addition, the Lp27 isolate exhibited an adhesion ratio of 9.5 ± 2.5% with Caco-2 cells. Antibiotic susceptibility tests indicated that the Lp27 isolate was sensitive to gentamicin, tetracycline, erythromycin, and chloramphenicol, and was resistant to vancomycin with a minimum inhibitory value of 23µg/mL. The Lp27 isolate inhibited cholesterol absorption through downregulation of Niemann-Pick C1-like 1 (NPC1L1) expression in Caco-2 cells. The Lp27 isolate was fed to hypercholesterolemic rats at a dose of 10(9) cfu/d for 4wk. The Lp27 feeding significantly lowered serum total cholesterol, low-density lipoprotein cholesterol, and triglycerides concentrations, but no change was observed in the serum high-density lipoprotein cholesterol concentrations. In addition, liver total cholesterol and triglycerides were decreased in the Lp27-fed group. The expression of NPC1L1 in the duodenum and jejunum was significantly decreased following Lp27 feeding. These results indicate that Lp27 might be an effective cholesterol-lowering probiotic and a possible mechanism for the cholesterol-reducing effects of probiotics.
authors.affiliation: Central Research Laboratory, Second Hospital of Jilin University, Changchun 130041, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.forename: Yujie
authors.initials: Y
authors.lastname: Sui
authors.forename: Longfei
authors.initials: L
authors.lastname: Yang
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018938:Caco-2 Cells / Q000378:metabolism; D002784:Cholesterol / Q000097:blood* / Q000378:metabolism; D043302:Cultured Milk Products / Q000382:microbiology*; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D018609:Tibet; D014280:Triglycerides / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C410800:NPC1L1 protein, human; D014280:Triglycerides; D002784:Cholesterol