title: How can genipin assist gelatin/carbohydrate chitosan scaffolds to act as replacements of load-bearing soft tissues?
abstract: In the engineering of soft tissues, designing the scaffolds with adequate strength and controllable biodegradation properties are essential. To fulfill such design criteria gelatin/carbohydrate chitosan (G/CC) scaffolds have gained much attention in engineering replacement of soft tissues. In this research, the influence of different cross-linking methods and parameters with genipin on the physico-chemical properties of G/CC scaffolds was investigated in detail. The scaffolds with different concentrations of G and CC were prepared and cross-linked trough two different methods, and the impact of cross-linker concentration beside of cross-linking time and temperature were fully analyzed. The obtained results suggested that the 1% genipin-cross-linked G60/CC40 scaffolds, prepared at room temperature for 24h through scaffold cross-linking method, could be a promising replacement in missing segments of load-bearing soft tissues. This article describes a systematic study of genipin cross-linking effects on G/CC scaffolds.
authors.affiliation: Sports Engineering Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran.
authors.forename: Melika
authors.initials: M
authors.lastname: Sarem
authors.forename: Fathollah
authors.initials: F
authors.lastname: Moztarzadeh
authors.forename: Masoud
authors.initials: M
authors.lastname: Mozafari
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D055119:Elastic Modulus; D005780:Gelatin / Q000737:chemistry* / Q000648:ultrastructure; D039823:Iridoids / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D016474:Weight-Bearing
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D039823:Iridoids; D014867:Water; D005780:Gelatin; D048271:Chitosan; C007834:genipin