title: Selective adsorption of hemoglobin using polymer-grafted-magnetite nanocellulose composite.
abstract: A novel adsorbent, poly(methacrylic acid-co-vinyl sulfonic acid)-grafted-magnetite nanocellulose composite (P(MAA-co-VSA)-g-MNCC) was synthesized for adsorbing hemoglobin (Hb) selectively from aqueous solutions. FTIR, XRD and DLS analyses were carried out to characterize the material. Hb exhibited a decrease in α-helix and an increase in β-sheet structure, upon immobilization onto P(MAA-co-VSA)-g-MNCC. The maximum adsorption was found to be at pH 6.5 with a monolayer capacity of 248.19mg/g at 30°C. Adsorption capacity attained saturation within 2h. The kinetic data were found to follow pseudo-second-order model which is based on chemisorption. Adsorption behavior was observed to be endothermic in nature. P(MAA-co-VSA)-g-MNCC can be used in the selective adsorption of Hb from mixture of proteins. Spent adsorbent was effectively regenerated with 0.01M KOH. Present investigation had shown that P(MAA-co-VSA)-g-MNCC would be a promising material for the selective recovery of Hb from aqueous solutions.
authors.affiliation: Department of Chemistry, University of Kerala, Kariavattom, Trivandrum-695 581, India. tsani@rediffmail.com
authors.forename: Thayyath Sreenivasan
authors.initials: TS
authors.lastname: Anirudhan
authors.forename: Sylaja Raveendran
authors.initials: SR
authors.lastname: Rejeena
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis* / Q000737:chemistry; D006878:Hydroxides / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D031022:Mangifera / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D006454:Hemoglobins; D006878:Hydroxides; D058185:Magnetite Nanoparticles; D017680:Potassium Compounds; D012996:Solutions; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002482:Cellulose; C029943:potassium hydroxide