title: L-Carnitine Protection Against Cisplatin Nephrotoxicity In Rats: Comparison with Amifostin Using Quantitative Renal Tc 99m DMSA Uptake.
abstract: OBJECTIVE
  In this study, we aimed to investigate the cytoprotective effect of L-carnitine against cisplatin-induced nephrotoxicity and to compare its efficacy with that of amifostin by quantitative renal Tc 99m DMSA uptake.
  
  
  METHODS
  Male Wistar rats were randomly divided into six groups of six animals each. 1) Control (saline; 5 ml/kg intraperitoneally); 2) L-carnitine (CAR; 300 mg/kg intraperitoneally); 3) Amifostine (AMI; 200 mg /kg intraperitoneally); 4) Cisplatin (CIS;7 mg/kg intraperitoneally); 5) Cisplatin plus L-carnitine (CIS + CAR); 6) Cisplatin plus amifostine (CIS + AMI). L-carnitine and amifostine were injected 30 minutes before cisplatin in Group 5 and 6. Tc 99m DMSA, 7.4 MBq/0.2 ml, was injected through the tail vein 72 hours after the drug administration. Rats were killed and kidneys removed by dissection 2 hours after the injection of the radiopharmaceutical. The percentage of the injected dose per gram of kidney tissue (%ID/g) was calculated. Renal function was monitored by measuring BUN and plasma levels of creatinine. Lipid peroxidation and glutathione content were determined by measuring malondialdehyde (MDA) and reduced glutathione (GSH) in kidney tissue homogenates.
  
  
  RESULTS
  Tc 99m DMSA uptake per gram tissue of the kidney as %ID/g was 29.54±4.72, 29.86 ± 7.47 and 26.37 ± 4.54 in the control, CAR and AMI groups respectively. %ID/g was the lowest of all the groups, 11.60±3.59 (p<0.01), in the cisplatin group. Carnitine or amifostine administration 30 minutes before cisplatin injection resulted a significant increase in %ID/g, 21.28±7.73 and 18.97±3.24 respectively, compared to those of cisplatin-treated rats (p<0.002). A marked increase in plasma BUN and creatinine indicating nephrotoxicity and acute renal failure was observed in the cisplatin-treated group. MDA and GSH levels were concordant with cisplatin-induced oxidative stress in the kidney tissue.
  
  
  CONCLUSIONS
  The results showed that L-carnitine significantly attenuates the cisplatin-induced nephrotoxicity as amifostin.
  
  
  BACKGROUND
  None declared.
authors.affiliation: Adnan Menderes University, Department of Nuclear Medicine, School of Medicine, Aydin, Turkey.
authors.forename: Yakup
authors.initials: Y
authors.lastname: Yürekli
authors.forename: Perihan
authors.initials: P
authors.lastname: Unak
authors.forename: Ciğdem
authors.initials: C
authors.lastname: Yenisey
authors.forename: Türkan
authors.initials: T
authors.lastname: Ertay
authors.forename: Fazilet Zumrut
authors.initials: FZ
authors.lastname: Biber Müftüler
authors.forename: Emin İlker
authors.initials: Eİ
authors.lastname: Medine
keywords: Spermatic cord torsion; radionuclide imaging; cryptorchidism; inguinal hernia