title: 3-O-demethylswertipunicoside inhibits MPP⁺-induced oxidative stress and apoptosis in PC12 cells.
abstract: The 3-O-demethylswertipunicoside (3-ODS) is extracted from Swertia punicea. Recent study from our laboratory has demonstrated that the 3-ODS protects against oxidative toxicity and apoptosis in PC12 cells (Zhang, S.P., Du, X.G., Pu, X.P., 2010. Biol. Pharm. Bull. 33, 1529-1533). The aim of our study is to further investigate the neuroprotective mechanisms of 3-ODS in 1-methyl-4-phenylpyridinium (MPP(+))-induced neurotoxicity in PC12 cells. The results indicated that pre-treatment with 3-ODS significantly increased the cell viability compared with MPP(+) treatment. It also alleviated the oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) level and reactive oxygen specise (ROS) production. Moreover, 3-ODS also attenuated MPP(+)-induced apoptosis by inhibiting Bax and Bcl-2 expressions, activating caspase-9, caspase-3, poly (ADP-ribose) polymerase-1 (PARP-1) cleavage, apoptosis-inducing factor (AIF) translocation and α-synuclein expression. These results suggest that 3-ODS might has applications as a complementary medicine for the treatment of Parkinson's disease (PD) or other neurodegenerative diseases.
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Deng
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Pu
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D015153:Blotting, Western; D015259:Dopamine Agents / Q000633:toxicity*; D005960:Glucosides / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D018696:Neuroprotective Agents; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D021381:Protein Transport; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D044004:Xanthones / Q000494:pharmacology*; D051844:alpha-Synuclein / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C583860:3-O-demethylswertipunicoside; D051033:Apoptosis Inducing Factor; D015259:Dopamine Agents; D005960:Glucosides; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D044004:Xanthones; D051844:alpha-Synuclein; D051028:bcl-2-Associated X Protein; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D015655:1-Methyl-4-phenylpyridinium