title: Up-regulation of miR-182 expression in colorectal cancer tissues and its prognostic value.
abstract: OBJECTIVE
  Accumulating evidences indicate that dysregulated microRNAs (miRNA) are involved in cancer tumorigenesis and progression. In the present study, we evaluated the expression of miR-182 in colorectal cancer and adjacent noncancerous tissues and explored its associations with clinicopathological characteristics and prognosis.
  
  
  METHODS
  Quantitative real-time PCR was used to analyze the expression of miR-182 in 148 pairs of colorectal cancer and adjacent noncancerous tissues. The relationship between miR-182 expression and clinicopathological characteristics in colorectal cancer tissues was estimated using Mann-Whitney U test or Kruskal-Wallis test, as appropriate. We calculated the survival curves and prognostic values of each variable by the Kaplan-Meier method and Cox proportional hazards regression analysis, respectively.
  
  
  RESULTS
  The expression of miR-182 was found up-regulated in colorectal cancer tissues compared with adjacent noncancerous tissues (p < 0.001), and its up-regulation was significantly correlated with large tumor size (p = 0.016), positive regional lymph node metastasis (p = 0.008), and advanced tumor-node-metastasis stage (p = 0.020). Furthermore, Kaplan-Meier analysis demonstrated that high miR-182 expression predicted poor survival (p = 0.001), and Cox proportional hazards risk analysis indicated that miR-182 was an independent prognostic factor for colorectal cancer.
  
  
  CONCLUSIONS
  MiR-182 was up-regulated in colorectal cancer tissues and correlated with adverse clinical characteristics and poor prognosis, indicating that miR-182 might be involved in colorectal cancer progression and could be used as a potential prognostic biomarker and therapeutic target in the management of colorectal cancer.
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital, Shandong University, 107 Wenhua West Road, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Lutao
authors.initials: L
authors.lastname: Du
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wen
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Dong
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guixi
authors.initials: G
authors.lastname: Zheng
authors.forename: Chuanxin
authors.initials: C
authors.lastname: Wang
mesh_terms: D000368:Aged; D018938:Caco-2 Cells; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D035683:MicroRNAs; C538984:Mirn182 microRNA, human