title: miR-612 suppresses the invasive-metastatic cascade in hepatocellular carcinoma.
abstract: MicroRNAs (miRNAs) play a critical role in tumor metastasis. In this study, we identified a set of 32 miRNAs involved in hepatocellular carcinoma (HCC) metastasis. Among them, miR-612 was shown for the first time to have inhibitory effects on HCC proliferation, migration, invasion, and metastasis. AKT2 was verified to be one of the direct targets of miR-612, through which the epithelial-mesenchymal transition (EMT) and metastasis were inhibited. The level of miR-612 in HCC patients was inversely associated with tumor size, stage, EMT, and metastasis. Of particular importance, miR-612 is involved in both the initial and final steps of the metastatic cascade, by suppressing local invasion and distant colonization. The pleiotropic roles of miR-612 in the HCC metastatic cascade suggest that it could be an effective target for both early and advanced HCC.
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Tao
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Wan
authors.forename: Ling-Yao
authors.initials: LY
authors.lastname: Zeng
authors.forename: Lu
authors.initials: L
authors.lastname: Xie
authors.forename: Hui-Chuan
authors.initials: HC
authors.lastname: Sun
authors.forename: Lun-Xiu
authors.initials: LX
authors.lastname: Qin
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Zheng-Gang
authors.initials: ZG
authors.lastname: Ren
authors.forename: Yi-Xue
authors.initials: YX
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Fan
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Wu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005500:Follow-Up Studies; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D012334:RNA, Neoplasm; C494921:AKT2 protein, human; D051057:Proto-Oncogene Proteins c-akt