title: Epithelial cell culture from human adenoids: a functional study model for ciliated and secretory cells.
abstract: BACKGROUND
  Mucociliary transport (MCT) is a defense mechanism of the airway. To study the underlying mechanisms of MCT, we have both developed an experimental model of cultures, from human adenoid tissue of ciliated and secretory cells, and characterized the response to local chemical signals that control ciliary activity and the secretion of respiratory mucins in vitro.
  
  
  METHODS
  In ciliated cell cultures, ciliary beat frequency (CBF) and intracellular Ca(2+) levels were measured in response to ATP, UTP, and adenosine. In secretory cultures, mucin synthesis and secretion were identified by using immunodetection. Mucin content was taken from conditioned medium and analyzed in the presence or absence of UTP.
  
  
  RESULTS
  Enriched ciliated cell monolayers and secretory cells were obtained. Ciliated cells showed a basal CBF of 10.7 Hz that increased significantly after exposure to ATP, UTP, or adenosine. Mature secretory cells showed active secretion of granules containing different glycoproteins, including MUC5AC.
  
  
  CONCLUSIONS
  Culture of ciliated and secretory cells grown from adenoid epithelium is a reproducible and feasible experimental model, in which it is possible to observe ciliary and secretory activities, with a potential use as a model to understand mucociliary transport control mechanisms.
authors.affiliation: Department of Otorhinolaryngology, Faculty of Medicine, Pontificia Universidad Católica de Chile, Marcoleta 350, 2°Piso, Santiago 8330033, Chile. clau_gonzalez@vtr.net
authors.forename: Claudia
authors.initials: C
authors.lastname: González
authors.forename: Marisol
authors.initials: M
authors.lastname: Espinosa
authors.forename: María Trinidad
authors.initials: MT
authors.lastname: Sánchez
authors.forename: Karla
authors.initials: K
authors.lastname: Droguett
authors.forename: Mariana
authors.initials: M
authors.lastname: Ríos
authors.forename: Ximena
authors.initials: X
authors.lastname: Fonseca
authors.forename: Manuel
authors.initials: M
authors.lastname: Villalón
mesh_terms: D000234:Adenoids / Q000166:cytology* / Q000378:metabolism*; D000241:Adenosine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002923:Cilia / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008954:Models, Biological; D055271:Mucin 5AC / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D014544:Uridine Triphosphate / Q000378:metabolism
chemical_list: C524678:MUC5AC protein, human; D055271:Mucin 5AC; D000255:Adenosine Triphosphate; D000241:Adenosine; D002118:Calcium; D014544:Uridine Triphosphate