title: Fluorescence imaging of flowing cells using a temporally coded excitation.
abstract: Imaging fluorescence in moving cells is fundamentally challenging because the exposure time is constrained by motion-blur, which limits the available signal. We report a method to image fluorescently labeled leukemia cells in fluid flow that has an effective exposure time of up to 50 times the motion-blur limit. Flowing cells are illuminated with a pseudo-random excitation pulse sequence, resulting in a motion-blur that can be computationally removed to produce near diffraction-limited images. This method enables observation of cellular organelles and their behavior in a fluid environment that resembles the vasculature.
authors.affiliation: Rowland Institute at Harvard, Harvard University, Cambridge, MA 02142, USA.
authors.forename: Sai Siva
authors.initials: SS
authors.lastname: Gorthi
authors.forename: Diane
authors.initials: D
authors.lastname: Schaak
authors.forename: Ethan
authors.initials: E
authors.lastname: Schonbrun
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D007938:Leukemia / Q000473:pathology*; D008029:Lighting / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation*