title: Regulation of IL-17 family members by adrenal hormones during experimental sepsis in mice.
abstract: Severe sepsis is a life-threatening disease that causes major morbidity and mortality. Catecholamines and glucocorticoids often have been used for the treatment of sepsis. Several recent studies have suggested a potential role of IL-17 during the development and progression of sepsis in small animal models. In this study, the cross-talk of catecholamines and glucocorticoids with members of the IL-17 family was investigated during sepsis in C57BL/6 mice. The concentrations in plasma of IL-17A, IL-17F, and the IL-17AF heterodimer all were increased greatly in mice after endotoxemia or cecal ligation and puncture as compared with sham mice. Surprisingly, when compared with IL-17A (487 pg/mL), the concentrations of IL-17F (2361 pg/mL) and the heterodimer, IL-17AF (5116 pg/mL), were much higher 12 hours after endotoxemia. After surgical removal of the adrenal glands, mice had much higher mortality after endotoxemia or cecal ligation and puncture. The absence of endogenous adrenal gland hormones (cortical and medullary) was associated with 3- to 10-fold higher concentrations of IL-17A, IL-17F, IL-17AF, and IL-23. The addition of adrenaline, noradrenaline, hydrocortisone, or dexamethasone to lipopolysaccharide-activated peritoneal macrophages dose-dependently suppressed the expression and release of IL-17s. The production of IL-17s required activation of c-Jun-N-terminal kinase, which was antagonized by both catecholamines and glucocorticoids. These data provide novel insights into the molecular mechanisms of immune modulation by catecholamines and glucocorticoids during acute inflammation.
authors.affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Bosmann
authors.forename: Fabien
authors.initials: F
authors.lastname: Meta
authors.forename: Robert
authors.initials: R
authors.lastname: Ruemmler
authors.forename: Mikel D
authors.initials: MD
authors.lastname: Haggadone
authors.forename: J Vidya
authors.initials: JV
authors.lastname: Sarma
authors.forename: Firas S
authors.initials: FS
authors.lastname: Zetoune
authors.forename: Peter A
authors.initials: PA
authors.lastname: Ward
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology*; D019446:Endotoxemia / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D020381:Interleukin-17 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018805:Sepsis / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D002395:Catecholamines; D005938:Glucocorticoids; D020381:Interleukin-17; D008070:Lipopolysaccharides; D048031:JNK Mitogen-Activated Protein Kinases