title: Bioactivity of tomato hybrid powder: antioxidant compounds and their biological activities.
abstract: The antioxidant and cytotoxic activities and the polyphenolic and anthocyanin contents of tomato hybrid powders were studied. Tomato powders were obtained, starting from the fresh fruits that had undergone an industrial process of drying and pulverization at two different temperatures. Antioxidant activities were evaluated in different extracts by using spectrophotometric assays: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid and N,N-dimethyl-p-phenylenediamine dihydrochloride cation radical inhibition for lipophilic and hydrophilic extracts, respectively, and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay for polyphenolic extracts. Moreover, polyphenolic and anthocyanin contents were also carried out to detect the presence of these bioactive compounds. The effect of cytotoxic activity in vitro of tomato hybrid powder extracts on T47D (human breast carcinoma) cells was also evaluated. Results showed good antioxidant activities in lipophilic, polyphenolic, and hydrophilic extracts of samples that were obtained at a lower temperature. Extracts of the sample obtained at a higher temperature presented moderate antioxidant activity, lower than the extracts of other samples, which was probably due to the loss of labile antioxidant compounds during the industrial process. Very interesting was the presence of anthocyanins in both samples, even if in traces, and also a moderate cytotoxicity of a lipophilic extract on T47D cells. Therefore, tomato hybrid powders, on the basis of their multifunctional properties, could have a biotechnological application in agri-food or cosmetic industries as an additive for improving nutritional and/or bioactive qualities of commercial products used in daily nutrition and cosmetics.
authors.affiliation: Institute of Biomolecular Chemistry, National Research Council of Italy, Pozzuoli, Italy. gtommonaro@icb.cnr.it
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Tommonaro
authors.forename: Rocco
authors.initials: R
authors.lastname: De Prisco
authors.forename: Gennaro Roberto
authors.initials: GR
authors.lastname: Abbamondi
authors.forename: Barbara
authors.initials: B
authors.lastname: Nicolaus
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002678:Chimera; D005260:Female; D005638:Fruit; D006801:Humans; D018551:Solanum lycopersicum / Q000737:chemistry*; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology* / Q000627:therapeutic use; D059808:Polyphenols / Q000494:pharmacology* / Q000627:therapeutic use; D011208:Powders
chemical_list: D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D028321:Plant Preparations; D059808:Polyphenols; D011208:Powders