title: Histological evaluation of tissue regeneration using biodegradable scaffold seeded by autologous cells for tubular/hollow organ applications.
abstract: The accurate interpretation of histological outcomes is a critical endpoint in preclinical studies. Thus, the toxicologic pathologist plays a vital role in conducting a comprehensive microscopic evaluation that would ultimately help defining the safety and functionality in Tissue Engineering/Regenerative Medicinal (TERM) products. In spite of many advances in regenerative medicine, there are no specific guidelines for the histological assessment of TERM products (Jayo et al. Toxicol Pathol 36:92-96, 2008). In this chapter, we describe the methodology designed to facilitate the detection of structural and functional changes when conducting a histological assessment including tissue collection (test article extraction), sampling, processing and fixation, special stains, statistical analysis, and morphometry.
authors.affiliation: Tengion Inc., Winston-Salem, NC, USA.
authors.forename: Elias A
authors.initials: EA
authors.lastname: Rivera
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Jayo
mesh_terms: D000818:Animals; D001837:Body Weights and Measures; D004285:Dogs; D023381:Endpoint Determination / Q000379:methods; D006652:Histological Techniques; D009130:Muscle, Smooth / Q000166:cytology; D038081:Organogenesis / Q000502:physiology*; D011100:Polyglycolic Acid; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014551:Urinary Tract / Q000166:cytology*
chemical_list: D011100:Polyglycolic Acid