title: Diagnostic and prognostic value of mitochondrial DNA minisatellites after stem cell transplantation.
abstract: Mitochondrial DNA has been used to investigate phylogenetic relationships and pathophysiologic roles in aging, degenerative diseases, and cancer. We investigated the prognostic usefulness of mitochondrial DNA minisatellite (mtMS) markers compared with nuclear short tandem repeat markers by evaluating the laboratory performance and clinical value of these markers in a large sample of patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT) with various simulated conditions in vitro and in serial follow-up samples. We examined the value of mtMS markers as a prognostic indicator in 100 patients with various hematologic disorders undergoing allo-HSCT, including 35 patients with longitudinal follow-up for 55 months. The mtMS markers showed high sensitivity and accuracy for the quantitative detection of chimerism compared with nuclear short tandem repeat markers, particularly in unrelated transplantation and under inappropriate sampling conditions. Longitudinal follow-up after allo-HSCT disclosed that chimerism precisely reflected the status of engraftment or relapse during the clinicopathological course. Moreover, changes in mtMS markers in recipients before allo-HSCT were associated with clinical outcomes. Our data indicate that mtMS markers have multiple functions in monitoring mixed chimerism and predicting prognosis after allo-HSCT.
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Won
authors.forename: Hye Ran
authors.initials: HR
authors.lastname: Kim
authors.forename: Hwan Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Hoon
authors.initials: H
authors.lastname: Kook
authors.forename: Hyeoung Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Shin
authors.forename: Soon Pal
authors.initials: SP
authors.lastname: Suh
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Ryang
authors.forename: Myung Geun
authors.initials: MG
authors.lastname: Shin
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000276:immunology; D005260:Female; D005500:Follow-Up Studies; D005819:Genetic Markers; D006084:Graft Rejection / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000401:mortality; D006085:Graft Survival / Q000235:genetics* / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007223:Infant; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D018598:Minisatellite Repeats; D008928:Mitochondria / Q000235:genetics* / Q000276:immunology; D011379:Prognosis; D012008:Recurrence; D016019:Survival Analysis; D018183:Transplantation Chimera / Q000235:genetics / Q000276:immunology*; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers