title: Engineering central metabolic modules of Escherichia coli for improving β-carotene production.
abstract: ATP and NADPH are two important cofactors for production of terpenoids compounds. Here we have constructed and optimized β-carotene synthetic pathway in Escherichia coli, followed by engineering central metabolic modules to increase ATP and NADPH supplies for improving β-carotene production. The whole β-carotene synthetic pathway was divided into five modules. Engineering MEP module resulted in 3.5-fold increase of β-carotene yield, while engineering β-carotene synthesis module resulted in another 3.4-fold increase. The best β-carotene yield increased 21%, 17% and 39% after modulating single gene of ATP synthesis, pentose phosphate and TCA modules, respectively. Combined engineering of TCA and PPP modules had a synergistic effect on improving β-carotene yield, leading to 64% increase of β-carotene yield over a high producing parental strain. Fed-batch fermentation of the best strain CAR005 was performed, which produced 2.1g/L β-carotene with a yield of 60mg/g DCW.
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Xinna
authors.initials: X
authors.lastname: Zhu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Xu
authors.forename: Jinlei
authors.initials: J
authors.lastname: Tang
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D009249:NADP / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D019207:beta Carotene / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D029968:Escherichia coli Proteins; D019207:beta Carotene; D009249:NADP; D000255:Adenosine Triphosphate