title: Human papillomaviruses modulate microRNA 145 expression to directly control genome amplification.
abstract: Human papillomaviruses (HPVs) modulate expression of host microRNAs. Our deep-sequencing analysis of organotypic raft cultures identified microRNA 145 (miR-145) as a differentiation-dependent microRNA that has functionally active target sequences in the HPV-31 E1 and E2 open reading frames. Overexpression of miR-145 in HPV-positive cells resulted in reduced genome amplification and late gene expression, along with decreased levels of cellular transcription factor KLF-4. Our studies show that HPV modulates miR-145 expression to control its own life cycle.
authors.affiliation: Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
authors.forename: Vignesh
authors.initials: V
authors.lastname: Gunasekharan
authors.forename: Laimonis A
authors.initials: LA
authors.lastname: Laimins
mesh_terms: D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D027383:Papillomaviridae / Q000502:physiology*; D014779:Virus Replication
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs