title: [Determination of nine triterpenoid acids from Ganoderma lucidum of different producting areas by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for determining nine triterpenes contained in Ganoderma lucidum.
  
  
  METHODS
  Chromatography conditions: Alltima C18 (4.6 mm x 150 mm, 5 microm) was adopted as the chromatographic column, with acetonitrile-0.04% formic acid solution as the mobile phase. The detective wavelength was set at 254 nm, and the column temperature was 15 degrees C.
  
  
  RESULTS
  The linearities of ganoderic acid C2, ganoderic acid G, ganoderenic acid B, ganoderic acid B, ganoderenic acid A, ganoderic acid A, lucideric acid A, ganoderenic acid D, and ganoderic acid C1 ranged between 6.81-40.88, 6.38-38.25, 6.75-40.50, 6.38-38.25, 5.95-35.65, 5.90-35.25, 7.00-42.00, 6.20-37.15 and 6.05-36.4 mg x L(-1) (r = 0.999 4, 0.999 2, 0.999 4, 0.999 2, 0.999 2, 0.994 5, 0.999 0, 0.999 2 and 0.998 4). Their recoveries were 102.1%, 102.3%, 100.6%, 103.3%, 104.1%, 103.2%, 96.42%, 102.5% and 101.5%, with RSD being 1.5%, 0.96%, 1.9%, 1.3%, 1.7%, 2.5%, 0.62%, 2.9% and 1.3%. The content of triterpenes contained in G. lucidum samples from 31 different areas and under different cultivation conditions.
  
  
  CONCLUSIONS
  The method is so feasible and highly reproducible that it can be used for quantitatie determination of the content of triterpenoid acid contained in G. lucidum.
authors.affiliation: Institute of Materia Medica, Peking Union Medical College, Chinese Academy of Medical Sciences, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing 100050, China.
authors.forename: Bao-Ming
authors.initials: BM
authors.lastname: Li
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Gu
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Kang
authors.forename: Chang-Hui
authors.initials: CH
authors.lastname: Wu
authors.forename: Ruo-Yun
authors.initials: RY
authors.lastname: Chen
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D020940:Reishi / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D014315:Triterpenes