title: Rice transglutaminase gene: Identification, protein expression, functionality, light dependence and specific cell location.
abstract: Transglutaminases (TGases), that catalyze post-translational modification of proteins, are scarcely known in plants. As part of a project to characterize transglutaminase genes in new plant species, the identification and characterization of a TGase in rice is presented. Using differential primers, a cDNA (tgo) of 1767bp from genomic rice DNA amplification was obtained. The primers were designed from the rice DNA sequence relatively homologous to the gene encoding active maize chloroplast TGase. Amino acid sequence of the deduced rice TGase protein (TGO) indicated that it contains the enzyme catalytic triad (Cys-His-Asp), three repeats, myristoylation domains and a leucine zipper motif. The TGO recombinant protein was characterized, showing specific activity regulation, and indicating that tgo encoded for an authentic TGase. Substrate preference and Ca(2+) dependent activity were also detected. In the rice plant TGO protein was immunolocalized in the grana chloroplasts, in protein vesicles near them, and in the bulliform cells. Immunoblot analyses, tgo mRNA expression, and TGase activity indicated that TGO expression in rice was light dependent and regulated by the illumination period. This work increases significantly our plant TGase understanding. Its functional role in rice, which is a good model system for C3 plants, is discussed.
authors.affiliation: Molecular Genetics Department, Centre for Research in Agricultural Genomics: CRAG (Consorci CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra, 08193 Barcelona, Spain. nefertiti.campos@cragenomica.es
authors.forename: N
authors.initials: N
authors.lastname: Campos
authors.forename: S
authors.initials: S
authors.lastname: Castañón
authors.forename: I
authors.initials: I
authors.lastname: Urreta
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.forename: J M
authors.initials: JM
authors.lastname: Torné
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002118:Calcium / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D018628:Gene Dosage; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006863:Hydrogen-Ion Concentration; D008027:Light; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects / Q000648:ultrastructure; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; D010940:Plant Proteins; D011994:Recombinant Proteins; D011503:Transglutaminases; D002118:Calcium