title: Lipoprotein lipase in chronic lymphocytic leukaemia - strong biomarker with lack of functional significance.
abstract: In chronic lymphocytic leukaemia (CLL), lipoprotein lipase (LPL) mRNA overexpression is an established poor prognostic marker, its function, however, is poorly understood. Measuring extracellular LPL enzymatic activity and protein, we found no difference between levels in CLL patients and those of controls, both before and after heparin treatment in vivo and in vitro. Investigating LPL knock down effects, we determined five potential downstream targets, of which one gene, STXBP3, reportedly is involved in fatty acid metabolism. While possibly reflecting an epigenetic switch towards an incorrect transcriptional program, LPL overexpression by itself does not appear to significantly influence CLL cell survival.
authors.affiliation: Department of Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Austria.
authors.forename: Edit
authors.initials: E
authors.lastname: Porpaczy
authors.forename: Stefanie
authors.initials: S
authors.lastname: Tauber
authors.forename: Martin
authors.initials: M
authors.lastname: Bilban
authors.forename: Gerhard
authors.initials: G
authors.lastname: Kostner
authors.forename: Michaela
authors.initials: M
authors.lastname: Gruber
authors.forename: Sandra
authors.initials: S
authors.lastname: Eder
authors.forename: Daniel
authors.initials: D
authors.lastname: Heintel
authors.forename: Trang
authors.initials: T
authors.lastname: Le
authors.forename: Karin
authors.initials: K
authors.lastname: Fleiss
authors.forename: Cathrin
authors.initials: C
authors.lastname: Skrabs
authors.forename: Medhat
authors.initials: M
authors.lastname: Shehata
authors.forename: Ulrich
authors.initials: U
authors.lastname: Jäger
authors.forename: Katrina
authors.initials: K
authors.lastname: Vanura
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D015331:Cohort Studies; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000097:blood / Q000235:genetics / Q000502:physiology*; D046228:Microarray Analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D034741:RNA, Small Interfering / Q000494:pharmacology; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D008071:Lipoprotein Lipase