title: Fabrication and characterization of bioactive β-Ca2SiO4/PHBV composite scaffolds.
abstract: A key challenge in tissue engineering is the construction of a scaffold with adequate properties which would mimic extracellular matrix (ECM) to induce the cells' efficient adhesion, proliferation and proper differentiation. Novel β-Ca2SiO4/PHBV composite scaffolds were fabricated by integrating β-Ca2SiO4 nanoparticles with PHBV backbone via a modified solvent casting-particulates leaching method, which generates interconnected porous structure and the high porosity, about 87%, of these scaffolds. Compared with PHBV scaffolds, β-Ca2SiO4/PHBV composite scaffolds facilitate the adhesion of human osteoblast-like MG-63 cells due to their increased hydrophilicity. The β-Ca2SiO4/PHBV composite scaffolds containing 2.5 or 5% β-Ca2SiO4 nanoparticles significantly enhance the proliferation of MG-63 cells by stimulating the transcription of the transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-7 (BMP-7) genes. These scaffolds also induce early differentiation via promoting the transcription of alkaline phosphatase (ALP). The results suggest the potential application of β-Ca2SiO4/PHBV composites in bone tissue engineering.
authors.affiliation: College of Materials Science and Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Nana
authors.initials: N
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Leilei
authors.initials: L
authors.lastname: Xia
authors.forename: Yao
authors.initials: Y
authors.lastname: Dai
authors.forename: Hairong
authors.initials: H
authors.lastname: Liu
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D017610:Calcium Compounds / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D017640:Silicates / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014158:Transcription, Genetic / Q000187:drug effects; D017155:Wettability; D014961:X-Ray Diffraction; D000094122:Polyhydroxybutyrates
chemical_list: D015415:Biomarkers; D017610:Calcium Compounds; D011091:Polyesters; D017640:Silicates; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C031293:calcium silicate