title: Ultrastructural alterations in the gills of Labeo rohita fingerlings exposed to thermal extremes.
abstract: This study aimed to determine the cellular alterations in the gill of Labeo rohita exposed to lethal temperature maxima (LTM ax ) and lethal temperature minima (LTM in ) by means of transmission electron microscopy (TEM). Acclimation of advanced fingerlings of L. rohita was carried out at 26°C for 30 days. Acclimated fish were subjected to a constant rate of increase or decrease in temperature (0.3°C/min) until the LTM ax and LTM in values were reached. Dissected gills were processed for TEM, both at the end of acclimation period at ambient temperature (26°C) and at lethal temperatures. Results indicated that at ambient temperature, the gill tissues appeared normal. However, significant changes were observed at lethal temperatures. The gill tissues at lethal temperature maxima showed severely damaged lamellae, with more vacuolated space. At lethal temperature minima, gill tissues showed increased density of mitochondria. Our prima-facie report indicated that L. rohita exposed to lethal temperatures exhibited marked ultrastructural changes in the gills.
authors.affiliation: Department of Zoology, Raja N. L. Khan Womens' College, Paschim Medinipur, 721102, West Bengal, India; Division of Fish Nutrition Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India.
authors.forename: T
authors.initials: T
authors.lastname: Das
authors.forename: N P
authors.initials: NP
authors.lastname: Sahu
authors.forename: S K
authors.initials: SK
authors.lastname: Chakraborty
authors.forename: N
authors.initials: N
authors.lastname: Chatterjee
authors.forename: M S
authors.initials: MS
authors.lastname: Mohammed
authors.forename: R S
authors.initials: RS
authors.lastname: Dalvi
authors.forename: K
authors.initials: K
authors.lastname: Baruah
authors.forename: A K
authors.initials: AK
authors.lastname: Pal
mesh_terms: D000818:Animals; D002347:Carps / Q000033:anatomy & histology*; D004721:Endoplasmic Reticulum; D004781:Environmental Exposure / Q000009:adverse effects; D005880:Gills / Q000033:anatomy & histology* / Q000166:cytology*; D006358:Hot Temperature / Q000009:adverse effects*; D046529:Microscopy, Electron, Transmission / Q000662:veterinary*; D008928:Mitochondria