title: Fluorescent imaging for assessment of the effect of combined application of electroporation and rifampicin on HaCaT cells as a new therapeutic approach for psoriasis.
abstract: The study aimed to clarify the role of electric pulses in combination with chemotherapy on the viability of keratinocyte cell line HaCaT, in the context of its application as a new therapeutic approach for psoriasis. The data show that electroporation of HaCaT cells in combination with rifampicin induces cytoskeleton disruption and increases permeability of cell monolayer due to cell-cell junctions' interruption, visualized by fluorescent imaging of E-cadherin and actin integrity. This was accompanied with synergistic reduction of cell viability. The study proposes a new opportunity for more effective skin treatment than chemotherapy. The future application of this electrochemotherapeutic approach for combined local treatment of psoriasis may have serous benefits because of a high possibility to avoid side-effects of conventional chemotherapy.
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria. nikolova@bio21.bas.bg
authors.forename: Biliana
authors.initials: B
authors.lastname: Nikolova
authors.forename: Anelia
authors.initials: A
authors.lastname: Kostadinova
authors.forename: Borislav
authors.initials: B
authors.lastname: Dimitrov
authors.forename: Zhivko
authors.initials: Z
authors.lastname: Zhelev
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
authors.forename: Ichio
authors.initials: I
authors.lastname: Aoki
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Saga
authors.forename: Iana
authors.initials: I
authors.lastname: Tsoneva
mesh_terms: D015820:Cadherins / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003131:Combined Modality Therapy; D018274:Electroporation / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D008870:Microtubules / Q000187:drug effects; D010539:Permeability; D011565:Psoriasis / Q000188:drug therapy* / Q000473:pathology; D012293:Rifampin / Q000008:administration & dosage*
chemical_list: D015820:Cadherins; D005456:Fluorescent Dyes; D012293:Rifampin