title: Tetrahymena functional genomics database (TetraFGD): an integrated resource for Tetrahymena functional genomics.
abstract: The ciliated protozoan Tetrahymena thermophila is a useful unicellular model organism for studies of eukaryotic cellular and molecular biology. Researches on T. thermophila have contributed to a series of remarkable basic biological principles. After the macronuclear genome was sequenced, substantial progress has been made in functional genomics research on T. thermophila, including genome-wide microarray analysis of the T. thermophila life cycle, a T. thermophila gene network analysis based on the microarray data and transcriptome analysis by deep RNA sequencing. To meet the growing demands for the Tetrahymena research community, we integrated these data to provide a public access database: Tetrahymena functional genomics database (TetraFGD). TetraFGD contains three major resources, including the RNA-Seq transcriptome, microarray and gene networks. The RNA-Seq data define gene structures and transcriptome, with special emphasis on exon-intron boundaries; the microarray data describe gene expression of 20 time points during three major stages of the T. thermophila life cycle; the gene network data identify potential gene-gene interactions of 15 049 genes. The TetraFGD provides user-friendly search functions that assist researchers in accessing gene models, transcripts, gene expression data and gene-gene relationships. In conclusion, the TetraFGD is an important functional genomic resource for researchers who focus on the Tetrahymena or other ciliates. Database URL: http://tfgd.ihb.ac.cn/
authors.affiliation: Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.forename: Yuming
authors.initials: Y
authors.lastname: Lu
authors.forename: Jinmei
authors.initials: J
authors.lastname: Feng
authors.forename: Dongxia
authors.initials: D
authors.lastname: Yuan
authors.forename: Miao
authors.initials: M
authors.lastname: Tian
authors.forename: Yue
authors.initials: Y
authors.lastname: Chang
authors.forename: Chengjie
authors.initials: C
authors.lastname: Fu
authors.forename: Guangying
authors.initials: G
authors.lastname: Wang
authors.forename: Honghui
authors.initials: H
authors.lastname: Zeng
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D053263:Gene Regulatory Networks / Q000235:genetics; D023281:Genomics; D020407:Internet; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA; D013768:Tetrahymena / Q000235:genetics*