title: Biodistribution of bone marrow mononuclear cells after intra-arterial or intravenous transplantation in subacute stroke patients.
abstract: OBJECTIVE
  To assess the biodistribution of bone marrow mononuclear cells (BMMNC) delivered by different routes in patients with subacute middle cerebral artery ischemic stroke.
  
  
  METHODS
  This was a nonrandomized, open-label Phase I clinical trial. After bone marrow harvesting, BMMNCs were labeled with technetium-99m and intra-arterially or intravenously delivered together with the unlabeled cells. Scintigraphies were carried out at 2 and 24 h after cell transplantation. Clinical follow-up was continued for 6 months.
  
  
  RESULTS
  Twelve patients were included, between 19 and 89 days after stroke, and received 1-5 × 10(8) BMMNCs. The intra-arterial group had greater radioactive counts in the liver and spleen and lower counts in the lungs at 2 and 24 h, while in the brain they were low and similar for both routes.
  
  
  CONCLUSIONS
  BMMNC labeling with technetium-99m allowed imaging for up to 24 h after intra-arterial or intravenous injection in stroke patients.
authors.affiliation: Department of Radiology, School of Medicine, Universidade Federal do Rio de Janeiro, Rua Professor Rodolpho Paulo Rocco 255, Cidade Universitária, Ilha do Fundão, 21941-913, Rio de Janeiro, Brazil.
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Rosado-de-Castro
authors.forename: Felipe da Rocha
authors.initials: Fda R
authors.lastname: Schmidt
authors.forename: Valeria
authors.initials: V
authors.lastname: Battistella
authors.forename: Sergio Augusto
authors.initials: SA
authors.lastname: Lopes de Souza
authors.forename: Bianca
authors.initials: B
authors.lastname: Gutfilen
authors.forename: Regina Coeli dos Santos
authors.initials: RC
authors.lastname: Goldenberg
authors.forename: Tais Hanae
authors.initials: TH
authors.lastname: Kasai-Brunswick
authors.forename: Leandro
authors.initials: L
authors.lastname: Vairo
authors.forename: Rafaella Monteiro
authors.initials: RM
authors.lastname: Silva
authors.forename: Eduardo
authors.initials: E
authors.lastname: Wajnberg
authors.forename: Pedro Emmanuel
authors.initials: PE
authors.lastname: Alvarenga Americano do Brasil
authors.forename: Emerson Leandro
authors.initials: EL
authors.lastname: Gasparetto
authors.forename: Angelo
authors.initials: A
authors.lastname: Maiolino
authors.forename: Soniza Vieira
authors.initials: SV
authors.lastname: Alves-Leon
authors.forename: Charles
authors.initials: C
authors.lastname: Andre
authors.forename: Rosalia
authors.initials: R
authors.lastname: Mendez-Otero
authors.forename: Gabriel
authors.initials: G
authors.lastname: Rodriguez de Freitas
authors.forename: Lea Mirian
authors.initials: LM
authors.lastname: Barbosa da Fonseca
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D006801:Humans; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D011877:Radionuclide Imaging; D020521:Stroke / Q000000981:diagnostic imaging / Q000628:therapy*; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon