title: Investigation of an on-line detection method combining near infrared spectroscopy with local partial least squares regression for the elution process of sodium aescinate.
abstract: An on-line detection method combining near infrared (NIR) spectroscopy with local partial least squares regression (PLSR) was investigated for the elution process of sodium aescinate. A 2 mm pathlength flow cell which transmitted NIR radiation through two fiber optic probes was placed in bypass of the macroporous resin column to collect real-time spectra of the sodium aescinate eluate. To compare the predictive accuracy, both local and global PLSR were employed to build mathematical models between NIR spectra and reference values. Meanwhile, Mahalanobis distance was introduced to select the appropriate local model for the prediction of unknown samples. Experimental results demonstrated that local PLSR was superior to global PLSR in both calibration performance and predictive accuracy. Moreover, the on-line detection method was proven to be feasible in real application and thereby would be of great value for monitoring the elution process of sodium aescinate in real time as well as determining the start and end points of eluate collection.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jin
authors.forename: Haiying
authors.initials: H
authors.lastname: Ding
authors.forename: Xuesong
authors.initials: X
authors.lastname: Liu
authors.forename: Xinmin
authors.initials: X
authors.lastname: Wan
authors.forename: Lianjun
authors.initials: L
authors.lastname: Luan
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D031319:Aesculus / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D004867:Equipment Design; D016018:Least-Squares Analysis; D012503:Saponins / Q000302:isolation & purification*; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods*; D014315:Triterpenes / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C584713:sodium aescinate