title: Simplified stereological evaluation of renal morphology after unilateral ureteral obstruction.
abstract: OBJECTIVE
  This study established a simple stereological method to obtain quantitative information about two- or three-dimensional structures based on observations from kidney sections in the unilateral ureteral obstruction(UUO) model.
  
  
  RESULTS
  Tubulointerstitial area(TA) and TA/the area of a rectangular field(RA) were raised gradually, but significantly, in the obstructed kidney from 1 to 3months post-ligation in comparison to the sham kidney of sham-operated rats(SOR). On the contrary, glomerular area(GA) and glomerular volume(GV) were decreased progressively over time, but significantly, in the obstructed kidney from 3weeks to 3months post-ligation compared to the sham kidney of SOR. UUO caused a progressive decline of TA and TA/RA in the contralateral kidney. More specifically, there were significant decreases in TA at 1,2,3months post-ligation, while in TA/RA only at 3months post-ligation in comparison to the right kidney of SOR. In contrast, GA and GV enhanced in a time-dependent manner in the contralateral kidney, in which the difference in GA reached significance only at 3months post-ligation, whereas the difference in GV reached significance from 1 to 3months post-ligation when comparing with the right kidney of SOR.
  
  
  CONCLUSIONS
  Our results confirmed two typical features of obstructive nephropathy, including widen interstitial space and glomerular atrophy in the obstructed kidney, and compensatory growth of the contralateral kidney.
authors.affiliation: Department of Pediatrics, the First Affiliated Hospital of Anhui Medical University, No. 218 Ji-Xi Road, Hefei 230022, PR China. hupeng28@yahoo.com.cn
authors.forename: Peng
authors.initials: P
authors.lastname: Hu
authors.forename: Xue Qi
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Cai
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.forename: Ling
authors.initials: L
authors.lastname: Lu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xuan
authors.forename: Yuan Han
authors.initials: YH
authors.lastname: Qin
mesh_terms: D000818:Animals; D001284:Atrophy / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007668:Kidney / Q000473:pathology* / Q000601:surgery; D007678:Kidney Glomerulus / Q000473:pathology* / Q000601:surgery; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014517:Ureteral Obstruction / Q000473:pathology* / Q000601:surgery
keywords: Compensatory hypertrophy; Wistar rat.; kidney section; stereological method; unilateral ureteral obstruction