title: Chromoblastomycosis due to Fonsecaea pedrosoi and F. monophora in Cuba.
abstract: We report two cases of chromoblastomycosis due to Fonsecaea pedrosoi and F. monophora in otherwise healthy Cuban males. Direct microscopic examination of biopsies revealed muriform cells, the hallmark of chromoblastomycosis. The suspected agents were recovered in culture, identified on the basis of morphological criteria and confirmed by sequencing of the internal transcribed spacer regions of rDNA. Final treatment consisted of surgical excision. The patients were successfully cured since there was no relapse after a follow-up of more than a year. In vitro antifungal susceptibility testing of both isolates showed that itraconazole and posaconazole had potent activity. High MICs of amphotericin B (2 μg/ml), fluconazole (>64 μg/ml), anidulafungin (8 μg/ml) and caspofungin (8 μg/ml) were found.
authors.affiliation: Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. badalii@yahoo.com
authors.forename: Hamid
authors.initials: H
authors.lastname: Badali
authors.forename: Maydelin
authors.initials: M
authors.lastname: Fernández-González
authors.forename: Bita
authors.initials: B
authors.lastname: Mousavi
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Illnait-Zaragozi
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: González-Rodríguez
authors.forename: G Sybren
authors.initials: GS
authors.lastname: de Hoog
authors.forename: Jacques F
authors.initials: JF
authors.lastname: Meis
mesh_terms: D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D001706:Biopsy; D002862:Chromoblastomycosis / Q000175:diagnosis* / Q000382:microbiology* / Q000473:pathology; D003462:Cuba; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008853:Microscopy; D008875:Middle Aged; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology / Q000473:pathology
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer