title: Addressing the challenge of optimum polyhydroxyalkanoate harvesting: monitoring real time process kinetics and biopolymer accumulation using dielectric spectroscopy.
abstract: In this study, dielectric spectroscopy was utilised to evaluate and define the optimum harvesting time for polyhydroxyalkanoates (PHA) production. It is essential to harvest PHA at the optimum time during fermentation for maximum yield, otherwise cells start degrading. Two carbon sources (acetic and butyric acids) were used in laboratory based experiments and a number of samples were measured ex situ for PHA production. The real-time measured capacitance in addition of identifying the cells growth phase, it correlated very well with ex situ measured PHA produced within the cells. The probe has proven to be a useful tool to assess process kinetics, to monitor real-time cell growth, PHA produced and defining the optimum harvesting time.
authors.affiliation: Sustainable Environment Research Centre, Faculty of Health, Sport and Science, University of Glamorgan, Pontypridd CF37 1DL, Wales, UK. gkedia@glam.ac.uk
authors.forename: Gopal
authors.initials: G
authors.lastname: Kedia
authors.forename: Pearl
authors.initials: P
authors.lastname: Passanha
authors.forename: Richard M
authors.initials: RM
authors.lastname: Dinsdale
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Guwy
authors.forename: Matthew
authors.initials: M
authors.lastname: Lee
authors.forename: Sandra R
authors.initials: SR
authors.lastname: Esteves
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D000332:Aerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques; D018533:Biomass; D020148:Butyric Acid / Q000494:pharmacology; D003199:Computer Systems; D020118:Cupriavidus necator / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D058266:Dielectric Spectroscopy / Q000379:methods*; D025802:Electric Capacitance; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*
chemical_list: D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D020148:Butyric Acid; D019342:Acetic Acid; D010100:Oxygen