title: Study on therapeutic action of bone marrow derived mesenchymal stem cell combined with vitamin E against acute kidney injury in rats.
abstract: OBJECTIVE
  The study aims to investigate the effect to treat acute kidney injury (AKI) with bone marrow derived mesenchymal stem cells (BMSCs) combined with vitamin E and to develop a new treatment mode for AKI preclinical study.
  
  
  METHODS
  BMSCs were separated from rat bone marrow. Gentamicin was used as a damage factor in the culture of renal tubular epithelial cells (RTECs) in vitro. After co-cultured with BMSCs and vitamin E, cell proliferation of each group was detected with CCK-8. In vivo, BMSCs (3.3×10(6)cells/kg) combined with vitamin E (80mg/kg) were administered in AKI rats induced by gentamicin intravenously. The pathological changes, biochemical parameters and apoptosis genes after treatment were investigated furthermore.
  
  
  RESULTS
  In co-cultured system, proliferating ability of RTECs was improved by BMSCs or vitamin E, especially for the combined group (P<0.05). The treated rats in combined group presented the lowest serum creatinine and the highest urea nitrogen compared to non-treated rats. The improvement in renal pathological changes was followed by less necrosis, degeneration and expansion of renal tubule. Under transmission electron microscope, unclear cell structure and reduction of endoplasmic reticulum in the cytoplasm of RTECs were ameliorated with the treatment. Most apoptosis genes were up-regulated in model group while down-regulated with the therapy. Further analysis showed that the two treatments may act independently with each other.
  
  
  CONCLUSIONS
  Our data demonstrated that both BMSC and vitamin E hold therapeutic action to AKI induced by gentamicin. Especially, the combined treatment is better than BMSC or vitamin E alone.
authors.affiliation: Department of Regeneration Medicine, School of Pharmaceutical, Jilin University, Changchun, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.forename: Yetong
authors.initials: Y
authors.lastname: Feng
authors.forename: Chao
authors.initials: C
authors.lastname: Dong
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.forename: Xuan
authors.initials: X
authors.lastname: Wu
authors.forename: Haoyu
authors.initials: H
authors.lastname: Wu
authors.forename: Peiheng
authors.initials: P
authors.lastname: Lv
authors.forename: Yulai
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology / Q000628:therapy*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis / Q000235:genetics; D001806:Blood Urea Nitrogen; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D018920:Coculture Techniques; D003131:Combined Modality Therapy; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D015536:Down-Regulation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005839:Gentamicins / Q000633:toxicity; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D014810:Vitamin E / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D005839:Gentamicins; D014810:Vitamin E; D003404:Creatinine