title: Bimodal optical diagnostics of oral cancer based on Rose Bengal conjugated gold nanorod platform.
abstract: Early detection of cancer often requires time consuming protocols and expensive instrumentation. To address these limitations, a Rose Bengal conjugated gold nanorod (RB-GNR) platform is developed for optical detection of cancer cells. The GNRs are modified by poly(allylamine hydrochloride) and conjugated with RB molecules to produce RB-GNRs which exhibit strong optical absorption in the near-infrared (NIR) region, good stability in aqueous solution, low cytotoxicity, and high specificity to oral cancer cells. The label-free sensing assay utilizes RB-GNRs as the sensing probe and by monitoring the aggregation-induced red-shift in the NIR absorption wavelength, specific and quantitative analysis of the oral cancer cell lysate is accomplished down to a detection limit of 2000 cells/mL. By employing the RB-GNRs as an imaging probe, an imaging assay is established on a home-made NIR absorption imaging system. Based on the NIR absorption by the RB-GNRs specifically conjugated with the oral cancer cells, multi-channel, rapid and quantitative detection of oral cancer cells is demonstrated. The high sensitivity and specificity of the RB-GNR platform as demonstrated by the two complementary assays provide non-invasive optical diagnostics of oral cancer cells enabling convenient screening and monitoring.
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China.
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Wang
authors.forename: Beike
authors.initials: B
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.forename: Tian-Ying
authors.initials: TY
authors.lastname: Sun
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Wang
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Yu
authors.forename: Chengzhang
authors.initials: C
authors.lastname: Li
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006046:Gold; D006801:Humans; D057446:Hydrodynamics; D007223:Infant; D008297:Male; D009062:Mouth Neoplasms / Q000175:diagnosis*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods*; D011073:Polyamines; D012395:Rose Bengal / Q000737:chemistry; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared; D055672:Static Electricity; D020349:Surface Plasmon Resonance; D013696:Temperature
chemical_list: D011073:Polyamines; D012395:Rose Bengal; C063994:polyallylamine; D006046:Gold