title: H₂-driven cofactor regeneration with NAD(P)⁺-reducing hydrogenases.
abstract: A large number of industrially relevant enzymes depend upon nicotinamide cofactors, which are too expensive to be added in stoichiometric amounts. Existing NAD(P)H-recycling systems suffer from low activity, or the generation of side products. H₂-driven cofactor regeneration has the advantage of 100% atom efficiency and the use of H₂ as a cheap reducing agent, in a world where sustainable energy carriers are increasingly attractive. The state of development of H₂-driven cofactor-recycling systems and examples of their integration with enzyme reactions are summarized in this article. The O₂-tolerant NAD⁺-reducing hydrogenase from Ralstonia eutropha is a particularly attractive candidate for this approach, and we therefore discuss its catalytic properties that are relevant for technical applications.
authors.affiliation: Department of Chemistry, University of Oxford, Oxford, UK.
authors.forename: Lars
authors.initials: L
authors.lastname: Lauterbach
authors.forename: Oliver
authors.initials: O
authors.lastname: Lenz
authors.forename: Kylie A
authors.initials: KA
authors.lastname: Vincent
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D055162:Biocatalysis; D019149:Bioreactors / Q000382:microbiology / Q000469:parasitology; D020118:Cupriavidus necator / Q000201:enzymology / Q000378:metabolism; D003901:Desulfovibrio / Q000201:enzymology / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D019163:Reducing Agents / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D019163:Reducing Agents; D009243:NAD; D009249:NADP; D006859:Hydrogen; D010088:Oxidoreductases; C093595:NADP-reducing hydrogenase, Desulfovibrio fructosovorans; C021376:hydrogen dehydrogenase