title: Analysis of gene expression in skin using laser capture microdissection.
abstract: Gene expression analysis is a useful tool to study the molecular mechanisms underlying skin development and homeostasis. Here we describe a method that utilizes laser capture microdissection (LCM) to isolate RNAs from localized areas of skin, allowing the characterization of gene expression by RT-PCR and microarray technologies.
authors.affiliation: Department of Biological Chemistry, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Briana
authors.initials: B
authors.lastname: Lee
authors.forename: Mikhail
authors.initials: M
authors.lastname: Geyfman
authors.forename: Bogi
authors.initials: B
authors.lastname: Andersen
authors.forename: Xing
authors.initials: X
authors.lastname: Dai
mesh_terms: D000328:Adult; D015870:Gene Expression / Q000502:physiology*; D018859:Hair Follicle / Q000166:cytology; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*