title: Strain-specific variations in cation content and transport in mouse erythrocytes.
abstract: Studies of ion transport pathophysiology in hematological disorders and tests of possible new therapeutic agents for these disorders have been carried out in various mouse models because of close functional similarities between mouse and human red cells. We have explored strain-specific differences in erythrocyte membrane physiology in 10 inbred mouse strains by determining erythrocyte contents of Na(+), K(+), and Mg(2+), and erythrocyte transport of ions via the ouabain-sensitive Na-K pump, the amiloride-sensitive Na-H exchanger (NHE1), the volume and chloride-dependent K-Cl cotransporter (KCC), and the charybdotoxin-sensitive Gardos channel (KCNN4). Our data reveal substantial strain-specific and sex-specific differences in both ion content and trans-membrane ion transport in mouse erythrocytes. These differences demonstrate the feasibility of identifying specific quantitative trait loci for erythroid ion transport and content in genetically standardized inbred mouse strains.
authors.affiliation: Department of Laboratory Medicine, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Rivera
authors.forename: Robert Y L
authors.initials: RY
authors.lastname: Zee
authors.forename: Seth L
authors.initials: SL
authors.lastname: Alper
authors.forename: Luanne L
authors.initials: LL
authors.lastname: Peters
authors.forename: Carlo
authors.initials: C
authors.lastname: Brugnara
mesh_terms: D000818:Animals; D002412:Cations / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D017136:Ion Transport; D008297:Male; D051379:Mice; D011188:Potassium / Q000097:blood; D012727:Sex Characteristics; D012964:Sodium / Q000097:blood; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013045:Species Specificity; D027981:Symporters / Q000378:metabolism; D000096922:K Cl- Cotransporters
chemical_list: D002412:Cations; D011188:Potassium; D012964:Sodium; D017923:Sodium-Hydrogen Exchangers; D000254:Sodium-Potassium-Exchanging ATPase; D027981:Symporters; D000096922:K Cl- Cotransporters
keywords: KCC; KCNN4; NHE1; Na-K pump; membrane transport