title: Shrink-induced sorting using integrated nanoscale magnetic traps.
abstract: We present a plastic microfluidic device with integrated nanoscale magnetic traps (NSMTs) that separates magnetic from non-magnetic beads with high purity and throughput, and unprecedented enrichments. Numerical simulations indicate significantly higher localized magnetic field gradients than previously reported. We demonstrated >20 000-fold enrichment for 0.001% magnetic bead mixtures. Since we achieve high purity at all flow-rates tested, this is a robust, rapid, portable, and simple solution to sort target species from small volumes amenable for point-of-care applications. We used the NSMT in a 96 well format to extract DNA from small sample volumes for quantitative polymerase chain reaction (qPCR).
authors.affiliation: Department of Biomedical Engineering, University of California, Irvine, California 92697, USA.
authors.forename: Dharmakeerthi
authors.initials: D
authors.lastname: Nawarathna
authors.forename: Nazila
authors.initials: N
authors.lastname: Norouzi
authors.forename: Jolie
authors.initials: J
authors.lastname: McLane
authors.forename: Himanshu
authors.initials: H
authors.lastname: Sharma
authors.forename: Nicholas
authors.initials: N
authors.lastname: Sharac
authors.forename: Ted
authors.initials: T
authors.lastname: Grant
authors.forename: Aaron
authors.initials: A
authors.lastname: Chen
authors.forename: Scott
authors.initials: S
authors.lastname: Strayer
authors.forename: Regina
authors.initials: R
authors.lastname: Ragan
authors.forename: Michelle
authors.initials: M
authors.lastname: Khine