title: Analysis of Salvinorin A in urine using microextraction in packed syringe and GC-MS/MS.
abstract: BACKGROUND
  The aim of this work was to develop and validate a method for the determination of Salvinorin A in human urine using microextraction by packed sorbent (MEPS) and GC-MS/MS.
  
  
  RESULTS
  The technique uses a sample volume as low as 0.2 ml, and the analyte was extracted using a C18 sorbent. The method showed to be linear between 20 and 1000 ng/ml and presented a LOD of 5 ng/ml. Intra- and inter-day precision and accuracy were acceptable. Absolute recoveries ranged from 71 to 80%.
  
  
  CONCLUSIONS
  GC-MS/MS with MEPS demonstrated to be a fast and simple procedure for the quantification of Salvinorin A in urine. This is the first time that GC-MS/MS with MEPS was used for the determination of this compound in biological fluids. Furthermore, the device could be reused for up to 80 extractions, which accounted for a lower cost of analysis.
authors.affiliation: CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6201-556 Covilhã, Portugal.
authors.forename: Ivo
authors.initials: I
authors.lastname: Moreno
authors.forename: Beatriz
authors.initials: B
authors.lastname: da Fonseca
authors.forename: David
authors.initials: D
authors.lastname: Oppolzer
authors.forename: Ana
authors.initials: A
authors.lastname: Martinho
authors.forename: Mário
authors.initials: M
authors.lastname: Barroso
authors.forename: Angelines
authors.initials: A
authors.lastname: Cruz
authors.forename: João António
authors.initials: JA
authors.lastname: Queiroz
authors.forename: Eugenia
authors.initials: E
authors.lastname: Gallardo
mesh_terms: D045785:Diterpenes, Clerodane / Q000302:isolation & purification / Q000592:standards / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000592:standards; D006801:Humans; D011786:Quality Control; D027544:Salvia / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D013594:Syringes; D054928:Validation Studies as Topic
chemical_list: D045785:Diterpenes, Clerodane; C090499:salvinorin A