title: Effect of newborn bovine serum on cryopreservation of adult bovine testicular tissue.
abstract: Bovine serum is widely used for cryopreservation of various cells and tissues. However, its cryoprotective effects on the cells and tissues are ambiguous and controversial. To test the effects of newborn calf serum (NCS) on cryopreservation of bovine testis tissue, NCS of 0%, 5%, 10% and 20% (v/v) was added into minimum essential medium + 10% dimethyl sulphoxide (DMSO)-based medium according to our previous report. Interestingly, the testicular cell viabilities and spermatogonia percentages from four groups were very close. The results indicated that an increase in the concentration of NCS in freezing medium to 20% has no significant effect on survival of both testicular cells and spermatogonia, and 10% DMSO-based freezing medium can maintain the testicular cell viability and spermatogonia percentage at a relatively high level (83.4 ± 0.7 and 56.5 ± 2.2 respectively). Taken together, NCS is dispensable for cryopreservation of adult bovine testis tissue. Our results provide an evidence for cutting down the costs in cryopreservation research of bovine testis tissue by reducing or giving up the use of serum.
authors.affiliation: Jilin Province Key Laboratory of Animal Embryo Engineering, The Center for Animal Embryo Engineering of Jilin Province, College of Veterinary Medicine, Jilin University, Changchun, China; Department of Laboratory Medicines, The 90th Hospital of Jinan, Jinan, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Wu
authors.forename: Y X
authors.initials: YX
authors.lastname: Sun
authors.forename: A B
authors.initials: AB
authors.lastname: Wang
authors.forename: G Y
authors.initials: GY
authors.lastname: Che
authors.forename: T J
authors.initials: TJ
authors.lastname: Hu
authors.forename: X M
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D002417:Cattle; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents; D008297:Male; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology; D013737:Testis / Q000166:cytology
chemical_list: D003451:Cryoprotective Agents
keywords: Bovine; cellular viability; cryopreservation; spermatogonia; testis tissue