title: The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array.
abstract: BACKGROUND
  Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.
  
  
  RESULTS
  In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.
  
  
  CONCLUSIONS
  We, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.
authors.affiliation: Genetic Epidemiology Laboratory, Department of Pathology, The University of Melbourne, Victoria, 3010, Australia.
authors.forename: JiHoon E
authors.initials: JE
authors.lastname: Joo
authors.forename: Ee Ming
authors.initials: EM
authors.lastname: Wong
authors.forename: Laura
authors.initials: L
authors.lastname: Baglietto
authors.forename: Chol-Hee
authors.initials: CH
authors.lastname: Jung
authors.forename: Helen
authors.initials: H
authors.lastname: Tsimiklis
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Park
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Wong
authors.forename: Dallas R
authors.initials: DR
authors.lastname: English
authors.forename: John L
authors.initials: JL
authors.lastname: Hopper
authors.forename: Gianluca
authors.initials: G
authors.lastname: Severi
authors.forename: Graham G
authors.initials: GG
authors.lastname: Giles
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Southey
mesh_terms: D058621:Blood Buffy Coat / Q000378:metabolism; D016000:Cluster Analysis; D018899:CpG Islands; D004247:DNA / Q000097:blood*; D019175:DNA Methylation; D059788:Dried Blood Spot Testing; D055106:Genome-Wide Association Study; D006801:Humans; D020407:Internet; D020411:Oligonucleotide Array Sequence Analysis; D012984:Software; D014584:User-Computer Interface
chemical_list: D004247:DNA