title: Expression of metalloproteinases and interleukins on anastomoses in septic rats.
abstract: BACKGROUND
  Anastomotic dehiscence is the most severe complication of colorectal surgery and its incidence increases in the presence of infection. It has been reported that immune factors or the activity of matrix metalloproteinases (MMP) may mediate the loss of anastomotic strength in the first postoperative days. In this study, we investigated the effects of abdominal sepsis on the MMP and interleukin (IL) gene expression in left colonic anastomoses in rats.
  
  
  METHODS
  Forty rats were divided into two groups of 20 animals according to the presence (group S) or absence (group N) of sepsis induction by cecal ligation and perforation during left colonic anastomosis. Each group was divided into subgroups for euthanasia on the third (N3 and S3) or seventh (N7 and S7) postoperative day (POD). A colonic segment containing anastomosis was removed for analysis of the expression of MMP1a, MMP8, MMP13, IL1β, IL6, IL10, TNFα, and IFNγ genes.
  
  
  RESULTS
  The anastomoses with abdominal sepsis showed increased MMP1a gene expression and decreased MMP8 gene expression both on the third and seventh POD. There was no change in the expression of MMP13. There was an increase in the expression of IL10 only on the third POD and a negative modulation of IL1β, IFNγ, and IL6 genes on both periods. The TNFα gene expression was negatively modulated on the third POD and became not modulated on the seventh POD.
  
  
  CONCLUSIONS
  Abdominal sepsis induced a specific inflammatory pattern with increased MMP1a and IL10 gene expression and negative modulation of MMP8, IL1β, IFNγ, and TNFα gene expression in left colonic anastomoses in rats.
authors.affiliation: Department of Surgical Clinics, School of Medicine, Universidade de Brasilia, Brasí lia, Brazil. silvismarques@yahoo.com.br
authors.forename: Silvana M
authors.initials: SM
authors.lastname: Silva
authors.forename: Márcio S
authors.initials: MS
authors.lastname: Jerônimo
authors.forename: Ildinete
authors.initials: I
authors.lastname: Silva-Pereira
authors.forename: Anamélia L
authors.initials: AL
authors.lastname: Bocca
authors.forename: João B
authors.initials: JB
authors.lastname: Sousa
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002432:Cecum / Q000293:injuries; D003106:Colon / Q000378:metabolism* / Q000601:surgery; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D008026:Ligation / Q000009:adverse effects; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D011677:Punctures / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1
keywords: Colon; Interleukin; Metalloproteinase; Rats; Sepsis; Surgical anastomosis