title: A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography coupled with pulsed amperometric detection.
abstract: A simultaneous analysis method was developed for Caihu-Dayuan-Yin decoction containing Bupleuri Radix, Glycyrrhizae Radix, and Ponciri fructus by reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD). The analytes were separated in a RP column with 0.01 % phosphoric acid-acetonitrile gradient elution. The limits of detection (S/N = 3) and of quantification (S/N = 10) ranged 0.1-3.0, and 0.3-10 ng, respectively. The intra- and interday precisions (RSDs) were ≤15.15 % and the average recoveries were 94.6-98.3 %. Our RP-HPLC-PAD method showed 1.7-100 times higher sensitivity than the RP-HPLC-UV method.
authors.affiliation: Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Ha-Jeong
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Ji-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Yong-Duk
authors.initials: YD
authors.lastname: Park
authors.forename: Seon-Pyo
authors.initials: SP
authors.lastname: Hong
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D055664:Electrochemical Techniques / Q000379:methods; D057230:Limit of Detection; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D010946:Plants, Medicinal; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; C584088:caihu-dayuan-yin