title: Increased expression of Dock180 protein in the noninfarcted myocardium in rats.
abstract: The integrin β1 subunit and its downstream molecule focal adhesion kinase have been identified as critical molecules for the inhibition of postinfarction cardiac remodeling, ischemic cardiomyopathy, and heart failure. However, as a component of the integrin pathway, it is still unclear whether Dock180 (dedicator of cytokinesis 1) protein is expressed in the noninfarcted myocardium of the peri-infarct zones. In this study, experimental myocardial infarction (MI) and sham-operation (sham) models were established in Sprague Dawley rats and the expression of Dock180 protein in the myocardium of the sham group and in the noninfarcted myocardium of the peri-infarct zones of the MI group was detected by Western blot technique. The Dock180 protein expression in the myocardium was as follows: postsham 24-hour group, 0.10 ± 0.04 (n = 8); post-MI 24-hour group, 0.13 ± 0.03 (n = 8); postsham 12-week group, 0.11 ± 0.05 (n = 8); and post-MI 12-week group 0.17 ± 0.04 (n = 8). The Dock180 protein expression in the myocardium in the post-MI 12-week group was significantly higher than that in the postsham 12-week group (p = 0.019), in the postsham 24-hour group (p = 0.004), and in the post-MI 24-hour group (p = 0.040). We conclude that Dock180 protein is expressed in the myocardium in rats. Furthermore, its expression is significantly increased in the noninfarcted myocardium of the peri-infarct zones.
authors.affiliation: Division of Cardiology, Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Wang
authors.forename: Wen-Ju
authors.initials: WJ
authors.lastname: Zhao
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wang
mesh_terms: D000818:Animals; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left; D020744:rac GTP-Binding Proteins / Q000032:analysis* / Q000502:physiology
chemical_list: C493871:DOCK180 protein, rat; D020744:rac GTP-Binding Proteins