title: Peptide Mass Fingerprinting and N-Terminal Amino Acid Sequencing of Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham.
abstract: A new cysteine protease named Nivulian-II has been purified from the latex of Euphorbia nivulia Buch.-Ham. The apparent molecular mass of Nivulian-II is 43670.846 Da (MALDI TOF/MS). Peptide mass fingerprint analysis revealed peptide matches to Maturase K (Q52ZV1_9MAGN) of Banksia quercifolia. The N-terminal sequence (DFPPNTCCCICC) showed partial homology with those of other cysteine proteinases of biological origin. This is the first paper to characterize a Nivulian-II of E. nivulia latex with respect to amino acid sequencing.
authors.affiliation: Department of Biotechnology, Faculty of Science, Post Graduate College of Science, Technology and Research, North Maharashtra University, Jalgaon, Maharashtra 425002, India ; Department of Biotechnology, Faculty of Science, Moolji Jaitha College, Jalgaon, Maharashtra 425002, India.
authors.forename: Shamkant B
authors.initials: SB
authors.lastname: Badgujar
authors.forename: Raghunath T
authors.initials: RT
authors.lastname: Mahajan