title: Tagging of biomolecules with deuterated water (D2O) in commercially important microalgae.
abstract: To understand the effect of any biomolecules in specific metabolic pathways in humans, bioavailability and for other basic understanding, stable isotopically-labelled biomolecules (preferably deuterated) is the fundamental pre-requisite. Production of deuterated biomolecules such as, astaxanthin, β-carotene, lutein, chlorophyll-a, and eicosapentaenoic acid (EPA, 20:5n-3) by metabolic tagging have been shown in commercially important microalgae, Haematococcus pluvialis and Phaeodactylum tricornutum. These microalgae were grown in appropriate optimized medium supplemented with 25% (v/v) deuterated water. LC-MS analysis showed a maximum of 20, 25, 23, 24, and 27% replacement of hydrogen by deuterium atoms respectively in astaxanthin, β-carotene, lutein, chlorophyll-a, and EPA. To our knowledge, this is the first report on the production of deuterated astaxanthin, chlorophyll-a and EPA by these microalgae.
authors.affiliation: Shannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, Limerick, Ireland. sushanta.saha@lit.ie
authors.forename: Sushanta Kumar
authors.initials: SK
authors.lastname: Saha
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Hayes
authors.forename: Siobhan
authors.initials: S
authors.lastname: Moane
authors.forename: Patrick
authors.initials: P
authors.lastname: Murray
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D017666:Deuterium Oxide / Q000378:metabolism; D017377:Diatoms / Q000378:metabolism*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D016823:Volvocida / Q000378:metabolism*
chemical_list: D001688:Biological Products; D003470:Culture Media; D017666:Deuterium Oxide