title: Multilaboratory study of the Biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial susceptibility and identification panels.
abstract: This study compared the Biomic automated well reader results to the MicroScan WalkAway results for reading MicroScan antimicrobial susceptibility and identification panels at four different sites. Routine fresh clinical isolates and quality control (QC) organisms were tested at each study site. A total of 46,176 MicroScan panel drug-organism combinations were read. The Biomic category agreement for 3,117 Gram-negative bacteria was 98.4%, with 1.4% minor and 0.2% major discrepancies. The Biomic category agreement for 5,233 Gram-positive bacteria was 98.7%, with 0.9% minor, 0.3% major, and 0.1% very major errors. Essential agreement, defined as Biomic results that were within ±1 2-fold dilution of the MicroScan results, was 99.3% for Gram-negative bacteria and 98.3% for Gram-positive bacteria. Biomic reading of MicroScan identification panels provided an overall agreement (first- and second-choice organism match) of 99.5% with 846 Gram-negative isolates and 99.5% with 430 Gram-positive isolates. These results suggest that the Biomic automated reader can provide accurate reading of MicroScan panels and has the capability of a visual panel read for manual adjustment of results.
authors.affiliation: Scott & White Healthcare, Temple, Texas, USA. rfader@sw.org
authors.forename: Robert C
authors.initials: RC
authors.lastname: Fader
authors.forename: Emily
authors.initials: E
authors.lastname: Weaver
authors.forename: Rhonda
authors.initials: R
authors.lastname: Fossett
authors.forename: Michele
authors.initials: M
authors.lastname: Toyras
authors.forename: John
authors.initials: J
authors.lastname: Vanderlaan
authors.forename: David
authors.initials: D
authors.lastname: Gibbs
authors.forename: Andrew
authors.initials: A
authors.lastname: Wang
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Thierjung
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*