title: Polyhydroxyalkanoic acids from structurally-unrelated carbon sources in Escherichia coli.
abstract: Polyhydroxyalkanoates (PHAs) that contain varied monomers with different chain lengths/structures were normally synthesized when a structurally-related precursor was present. The biosynthesis of PHAs from unrelated carbon sources in microorganisms including Escherichia coli met many challenges in the past. Recently, with the development of metabolic engineering and synthetic biology, the production of PHAs from unrelated carbon sources obtained a breakthrough. Polyesters containing 2-hydroxypropionate, 3-hydroxypropionate, 4-hydroxybutyrate, 3-hydroxyvalarate, and medium-chain-length 3-hydroxyalkanoate monomers can all be synthesized in E. coli by integrating exogenous or endogenous pathways and/or genes. This review will summarize the progresses in this area. In addition, the strategies that lead to the production of PHAs with varied monomers and high polymer content in the cell are discussed.
authors.affiliation: National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhuang
authors.forename: Quanfeng
authors.initials: Q
authors.lastname: Liang
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods; D009930:Organic Chemicals / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*
chemical_list: D009930:Organic Chemicals; D054813:Polyhydroxyalkanoates; D002244:Carbon