title: Transcriptome analysis of phytohormone, transporters and signaling pathways in response to vanadium stress in rice roots.
abstract: Trace concentrations of vanadium (V) have several benefits for plant growth, but high concentrations are toxic. To help characterize the cellular mechanisms underlying the toxic effects of V in plants, we present the first large-scale analysis of rice root responding to V during the early stages (1 and 3 h) of toxicity. Exposure to V triggered changes in the transcript levels of several genes related to cellular metabolic process, response to stimulus and transporters. Gene expression profiling revealed upregulated levels of genes associated with signaling and biosynthesis of auxin, abscisic acid (ABA) and jasmonic acid (JA) in V-treated rice roots. In addition, V upregulated the expression of ATP-dependent GSH-conjugated transport, ATP binding cassette (ABC) transporter, and markedly downregulated of the expression of divalent cation transporters, drug/metabolite transporter (DMT) and zinc-iron permease (ZIP). Among the V-specific responsive transcription factors and protein kinases, the most predominant families were NAC (NAM, ATAF, CUC) transcription factor, receptor-like cytoplasmic kinase VII (RLCK-VII) and leucine-rich repeat kinase VIII (LRR-VIII). These microarray data provide a new insight into the molecular mechanism of the rice roots response to V toxicity.
authors.affiliation: Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Lin
authors.forename: Ngoc Nam
authors.initials: NN
authors.lastname: Trinh
authors.forename: Chung-Wen
authors.initials: CW
authors.lastname: Lin
authors.forename: Hao-Jen
authors.initials: HJ
authors.lastname: Huang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000040:Abscisic Acid / Q000378:metabolism; D001692:Biological Transport; D003517:Cyclopentanes / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D012275:Oryza / Q000187:drug effects* / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000032:analysis* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014158:Transcription, Genetic; D059467:Transcriptome; D014639:Vanadium / Q000494:pharmacology*
chemical_list: D018528:ATP-Binding Cassette Transporters; D003517:Cyclopentanes; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014639:Vanadium; C011006:jasmonic acid; D000040:Abscisic Acid