title: PSP activates monocytes in resting human peripheral blood mononuclear cells: immunomodulatory implications for cancer treatment.
abstract: Polysaccharopeptide (PSP), from Coriolus versicolor, has been used as an adjuvant to chemotherapy, and has demonstrated anti-tumor and immunomodulating effects. However its mechanism remains unknown. To elucidate how PSP affects immune populations, we compared PSP treatments both with and without prior incubation in phytohaemagglutinin (PHA) - a process commonly used in immune population experimentation. We first standardised a capillary electrophoresis fingerprinting technique for PSP identification and characterisation. We then established the proliferative capability of PSP on various immune populations in peripheral blood mononuclear cells, using flow cytometry, without prior PHA treatment. It was found that PSP significantly increased the number of monocytes (CD14(+)/CD16(-)) compared to controls without PHA. This increase in monocytes was confirmed using another antibody panel of CD14 and MHCII. In contrast, proliferations of T-cells, NK, and B-cells were not significantly changed by PSP. Thus, stimulating monocyte/macrophage function with PSP could be an effective therapeutic intervention in targeting tumors.
authors.affiliation: The University of Sydney, NSW 2006, Australia. bhagwant.sekhon@sydney.edu.au
authors.forename: Bhagwant Kaur
authors.initials: BK
authors.lastname: Sekhon
authors.forename: Daniel Man-Yuen
authors.initials: DM
authors.lastname: Sze
authors.forename: Wing Keung
authors.initials: WK
authors.lastname: Chan
authors.forename: Kei
authors.initials: K
authors.lastname: Fan
authors.forename: George Qian
authors.initials: GQ
authors.lastname: Li
authors.forename: Douglas Edwin
authors.initials: DE
authors.lastname: Moore
authors.forename: Rebecca Heidi
authors.initials: RH
authors.lastname: Roubin
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D009369:Neoplasms / Q000276:immunology / Q000503:physiopathology / Q000628:therapy*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011509:Proteoglycans / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D055454:Trametes / Q000737:chemistry*
chemical_list: D007155:Immunologic Factors; D010835:Phytohemagglutinins; D011509:Proteoglycans; C093216:polysaccharide peptide