title: Fluorescent reporters and methods to analyze fluorescent signals.
abstract: The use of fluorescent reporters and the development of new imaging technologies have revolutionized studies in cell biology. During recent years the number of fluorescent proteins offering the ability to visualize the distribution of proteins, organelles, and cells has increased tremendously. In parallel, the imaging tools available were refined rapidly enabling now the use of a huge spectrum of specialized methods to explore the cellular and subcellular localization and dynamics of fluorescently tagged markers. This chapter presents an overview of fluorescent reporters and methods available, and describes a selection of those that are routinely applicable in imaging studies using Dictyostelium discoideum.
authors.affiliation: Institut für Anatomie und Zellbiologie, Ludwig-Maximilians-Universität München, Munich, Germany. amueller@lrz.uni-muenchen.de
authors.forename: Annette
authors.initials: A
authors.lastname: Müller-Taubenberger
authors.forename: Hellen C
authors.initials: HC
authors.lastname: Ishikawa-Ankerhold
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000465:Algorithms; D002633:Chemotaxis; D003627:Data Interpretation, Statistical; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D046210:Microfluidic Analytical Techniques; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D059010:Single-Cell Analysis
chemical_list: D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins