title: Effect of autologous platelet-rich plasma on distraction osteogenesis in the mandible of rabbits: a morphologic and morphometric approach.
abstract: Distraction osteogenesis of the jaw is a common surgical practice in the treatment of pediatric craniofacial deformities. Autologous platelet rich plasma (PRP) has been used to increase the healing potential of bones in humans during distraction osteogenesis. This article aims to study the morphometric and morphologic parameters resulting from the effect of PRP on bone healing after mandibular distraction in rabbits. Right mandibular distraction was performed in 12 rabbits divided equally into 2 groups. PRP and physiological saline were injected, according to a defined protocol, in the callus following distraction of the experimental and control groups respectively. The rabbits were sacrificed after a consolidation period of 45 days and the mandibles were surgically removed. Bone mineral density, radiographic analysis, mechanical properties and histological features of the lengthened bones were assessed using radiographic examination, dual X-ray absorptiometry, biomechanical testing and histology. Results showed that the regenerate bone density, the amount of trabeculation in addition to the bone mineral density and mineral content, as measured by absorptiometry, were better with PRP but not significantly different between groups. Two radiographs revealed a more consistent healing in the experimental mandibles compared with erratic outcomes in corresponding controls. Two of the latter could not be subjected to any mechanical testing because the mandibular parts, connected with fibrous tissue, were separated. Consequently, the biomechanical test depicted greater maximal loads in the experimental group. The histological studies exhibited more ossification and less connective tissue fibers in the experimental group. PRP accelerated healing of mandibles in rabbits following distraction and improved their biomechanical properties. These findings have significant clinical implications on reducing the period of consolidation of the mandibles which may not be immobilized like other bones for long periods of time.
authors.affiliation: Department of Pathology and Laboratory Medicine, American University of Beirut, Lebanon.
authors.forename: T
authors.initials: T
authors.lastname: Bou Assi
authors.forename: M
authors.initials: M
authors.lastname: Rahme
authors.forename: S
authors.initials: S
authors.lastname: Saghieh
authors.forename: N
authors.initials: N
authors.lastname: Bou Raad Azoury
authors.forename: I
authors.initials: I
authors.lastname: Abdallah Hajj Hussein
authors.forename: A
authors.initials: A
authors.lastname: Leone
authors.forename: A
authors.initials: A
authors.lastname: Jurjus
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D001696:Biomechanical Phenomena; D001861:Bone Regeneration; D019248:Jaw Fixation Techniques; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D019857:Osteogenesis, Distraction; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D011817:Rabbits; D014182:Transplantation, Autologous