title: In vivo monitoring of acetylcholine release from cardiac vagal nerve endings in anesthetized mice.
abstract: We applied a microdialysis technique to the left ventricular myocardium of anesthetized mice and tried to monitor acetylcholine (ACh) release from cardiac vagal nerves. Transection of bilateral cervical vagal nerves decreased dialysate ACh concentration. Electrical stimulation of the left cervical vagal nerve increased dialysate ACh concentration in proportion to the frequency of stimulation. Intravenous administration of hexamethonium, prevented the increase in dialysate ACh concentration during vagal nerve stimulation, indicating that ACh in the dialysate primarily reflects ACh released from post-ganglionic cardiac vagal nerves. Microdialysis permits monitoring of ACh release from post-ganglionic cardiac vagal nerves that are most likely to be innervating the left ventricle in mice.
authors.affiliation: Department of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan. dyzhan@ri.ncvc.go.jp
authors.forename: Dong-Yun
authors.initials: DY
authors.lastname: Zhan
authors.forename: Cheng-Kun
authors.initials: CK
authors.lastname: Du
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Akiyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Sonobe
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Tsuchimochi
authors.forename: Shuji
authors.initials: S
authors.lastname: Shimizu
authors.forename: Toru
authors.initials: T
authors.lastname: Kawada
authors.forename: Mikiyasu
authors.initials: M
authors.lastname: Shirai
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000758:Anesthesia / Q000379:methods; D000818:Animals; D004558:Electric Stimulation / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017551:Microdialysis / Q000379:methods*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D014630:Vagus Nerve / Q000378:metabolism*; D055536:Vagus Nerve Stimulation / Q000379:methods*
chemical_list: D018733:Nicotinic Antagonists; D000109:Acetylcholine