title: Phenotypic analysis of bioactive cells for application in regenerative medicine.
abstract: The following chapter outlines methodologies to phenotypically characterize primary cells for the use in tissue-engineered and regenerative medicine applications. Methods covered include analyzing cells using immunocytochemistry, fluorescence-activated cell sorting, and confocal microscopy of adherent and suspended cells, as well as combinations of formulated cell-biomaterial constructs.
authors.affiliation: Tengion Inc., Winston-Salem, NC, USA.
authors.forename: Teresa Bora
authors.initials: TB
authors.lastname: Burnette
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Bruce
mesh_terms: D004847:Epithelial Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007668:Kidney / Q000166:cytology*; D018613:Microscopy, Confocal; D010641:Phenotype; D061251:Primary Cell Culture / Q000379:methods*; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D001743:Urinary Bladder / Q000166:cytology*