title: Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model.
abstract: Cell sheet engineering has been noted as a new and valuable approach in the tissue-engineering field. The objective of this study was to explore a procedure to induce hepatic progenitor cells and biliary duct structures in the liver. Sprague-Dawley rat dermal fibroblast (DF) sheets were transplanted into the incised surface of the liver of F344 nude rats. In the control group, an incision was made without transplantation of the DF sheets. Bile duct (BD)-like structures and immature hepatocyte-like cells were observed in the DF sheet transplant sites. These BD-like structures were cytokeratin-8-positive, while the hepatocyte-like cells were both OV-6-positive and α-fetoprotein-positive as well. The proliferation and differentiation of liver progenitor cells were not influenced by hepatectomy. We also transplanted DF sheets transfected with a plasmid encoding the enhanced yellow fluorescent protein target to mitochondria (pEYFP-Mito) by electroporation, and found that the new structures were pEYFP-Mito-negative. We observed new BD-like structures and immature hepatocytes after transplantation of DF sheets onto incised liver surfaces, and clarified that the origin of these BD-like structures and hepatocyte-like cells was the recipient liver. The present study described an aspect of the hepatic differentiation process induced at the site of liver injury.
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Izumi
authors.initials: I
authors.lastname: Muraoka
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Mitsuhisa
authors.initials: M
authors.lastname: Takatsuki
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sakai
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Tomonaga
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Soyama
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hidaka
authors.affiliation: Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Japan.|Division of Molecular and Cellular Biology, Department of Anatomy, Faculty of Medicine, University of Miyazaki, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hishikawa
authors.affiliation: Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Koji
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Utoh
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ohashi
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanematsu
authors.affiliation: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Eguchi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology* / Q000473:pathology*; D008164:Luminescent Proteins / Q000737:chemistry; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011923:Rats, Nude; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D014184:Transplantation, Homologous
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D003094:Collagen
keywords: allogeneic rat model; cell sheet; dermal fibroblast; hepatic progenitor cell; liver regeneration; nude rats; temperature-responsive dishes; tissue engineering