title: Isolation of mouse hepatocytes.
abstract: Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital.
authors.affiliation: Institute of Structural and Molecular Biology, University College London, London, UK.
authors.forename: Mina
authors.initials: M
authors.lastname: Edwards
authors.forename: Lyndsey
authors.initials: L
authors.lastname: Houseman
authors.forename: Ian R
authors.initials: IR
authors.lastname: Phillips
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Shephard
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003094:Collagen / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004338:Drug Combinations; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000737:chemistry; D008099:Liver / Q000166:cytology; D051379:Mice; D010477:Perfusion; D011509:Proteoglycans / Q000737:chemistry; D013242:Sterilization; D014161:Transduction, Genetic
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D003577:Cytochrome P-450 Enzyme System