title: Suppression of charging effect on collagen fibrils utilizing a conductive probe in TEM.
abstract: We investigate the effectiveness of utilizing a conductive probe for a transmission electron microscope (TEM) to suppress charging caused by electron irradiation. To do this, the electric field around a charged collagen fibril was visualized by electron holography and then quantitatively analyzed by computer simulations. The electric field changed noticeably when the conductive probe was moved near the specimen and charging was drastically suppressed when the conductive probe directly touched the charged specimen. The causes of the change in the electric field and suppression of charging are briefly discussed.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.forename: Zentaro
authors.initials: Z
authors.lastname: Akase
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shindo
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000125:Achilles Tendon; D000818:Animals; D003094:Collagen / Q000032:analysis*; D003198:Computer Simulation; D004560:Electricity; D006696:Holography; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D012867:Skin
chemical_list: D003094:Collagen
keywords: charging effect; electric field; electron holography; piezo drive; probe; secondary electron