title: ATP induces NO production in hippocampal neurons by P2X(7) receptor activation independent of glutamate signaling.
abstract: To assess the putative role of adenosine triphosphate (ATP) upon nitric oxide (NO) production in the hippocampus, we used as a model both rat hippocampal slices and isolated hippocampal neurons in culture, lacking glial cells. In hippocampal slices, additions of exogenous ATP or 2'(3')-O-(4-Benzoylbenzoyl) ATP (Bz-ATP) elicited concentration-dependent NO production, which increased linearly within the first 15 min and plateaued thereafter; agonist EC50 values were 50 and 15 µM, respectively. The NO increase evoked by ATP was antagonized in a concentration-dependent manner by Coomassie brilliant blue G (BBG) or by N(ω)-propyl-L-arginine, suggesting the involvement of P2X7Rs and neuronal NOS, respectively. The ATP induced NO production was independent of N-methyl-D-aspartic acid (NMDA) receptor activity as effects were not alleviated by DL-2-Amino-5-phosphonopentanoic acid (APV), but antagonized by BBG. In sum, exogenous ATP elicited NO production in hippocampal neurons independently of NMDA receptor activity.
authors.affiliation: Centro de Envejecimiento y Regeneración, Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. jfcodocedo@gmail.com
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Codocedo
authors.forename: Juan Alejandro
authors.initials: JA
authors.lastname: Godoy
authors.forename: Maria Ines
authors.initials: MI
authors.lastname: Poblete
authors.forename: Nibaldo C
authors.initials: NC
authors.lastname: Inestrosa
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Huidobro-Toro
mesh_terms: D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D008568:Memory; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*; D012394:Rosaniline Dyes / Q000494:pharmacology; D015398:Signal Transduction
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D058486:Receptors, Purinergic P2X7; D012394:Rosaniline Dyes; D009569:Nitric Oxide; D018698:Glutamic Acid; C033901:3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate; D015763:2-Amino-5-phosphonovalerate; D000255:Adenosine Triphosphate; D052248:Nitric Oxide Synthase Type I; C004692:coomassie Brilliant Blue