title: Endogenous stromal cell-derived factor-1 (CXCL12) supports autonomous growth of acute myeloid leukemia cells.
abstract: We investigated the role of endogenous stromal cell-derived factor-1 (SDF-1; CXCL12) in the survival and proliferation of acute myeloid leukemia (AML) cells in vitro. CD34(+) cells from the peripheral blood of five patients with AML, as well as five AML cell lines, produced and secreted SDF-1. Knock-down of endogenous SDF-1 expression using siRNA technology downregulated the constitutive phosphorylation of SDF-1-related signaling molecules and significantly inhibited spontaneous proliferation of the AML cell lines during a 3-day incubation in serum-free conditions. These results indicate that endogenous SDF-1 expression by AML cells plays a role in the autonomous growth of the cells.
authors.affiliation: Department of Internal Medicine, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Ha-Yon
authors.initials: HY
authors.lastname: Kim
authors.forename: Yoon-Suk
authors.initials: YS
authors.lastname: Oh
authors.forename: Ik-Chan
authors.initials: IC
authors.lastname: Song
authors.forename: Seong-Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hwan-Jung
authors.initials: HJ
authors.lastname: Yun
authors.forename: Samyong
authors.initials: S
authors.lastname: Kim
authors.forename: Deog-Yeon
authors.initials: DY
authors.lastname: Jo
mesh_terms: D049109:Cell Proliferation; D054377:Chemokine CXCL12 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D055785:Gene Knockdown Techniques; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010766:Phosphorylation / Q000235:genetics; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D020298:U937 Cells
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D009363:Neoplasm Proteins