title: In vitro inhibition of bovine enamel demineralization by enamel matrix derivative.
abstract: This study aimed to determine whether enamel matrix derivative (Emdogain) affects the demineralization of bovine enamel in vitro and to assess the agent's anti-caries potential. Bovine enamel blocks were prepared and randomly divided into three groups (n = 15 per group), which were treated with distilled water (negative control), NaF (positive control), or Emdogain. All three groups were pH-cycled 12 times over 6 days. The percentage of surface enamel microhardness reduction (%SMHR), calcium demineralization rate (CDR), surface roughness, lesion depth and mineral loss after demineralization were examined. Surface morphology of specimens was studied by scanning electron microscopy. The Emdogain and positive control groups showed similar surface roughness, lesion depths and mineral loss, which were significantly lower than those in the negative control group. In addition, the enamel surfaces of both the Emdogain and NaF groups showed much narrower intercrystalline spaces than the surfaces of the negative control group, which exhibited extensive microfractures along the crystal edges. %SMHR differed significantly among all three groups, with the smallest value in the Emdogain group and the greatest in the negative control group. These results indicate that enamel matrix derivative (Emdogain) can significantly inhibit demineralization of bovine enamel in vitro, suggesting that it has potential as an anti-caries agent.
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Jin Mei
authors.initials: JM
authors.lastname: Ran
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Cheng Cheng
authors.initials: CC
authors.lastname: Ieong
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Chen Yang
authors.initials: CY
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Xue Ping
authors.initials: XP
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Xue Dong
authors.initials: XD
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P.R. China.
authors.forename: Ling Lin
authors.initials: LL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003731:Dental Caries / Q000517:prevention & control / Q000662:veterinary*; D003743:Dental Enamel / Q000187:drug effects*; D003746:Dental Enamel Proteins / Q000008:administration & dosage*; D007180:Incisor / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D013499:Surface Properties / Q000187:drug effects; D017001:Tooth Demineralization / Q000517:prevention & control / Q000662:veterinary*; D016896:Treatment Outcome
chemical_list: D003746:Dental Enamel Proteins; C109069:enamel matrix proteins
keywords: atomic force microscope; caries; demineralization; emdogain; enamel matrix derivative; polarized microscopy; scanning electron microscopy; transverse microradiography