title: Use of mixed micelles for presentation of building blocks in a new combinatorial discovery methodology: proof-of-concept studies.
abstract: We describe a new method of combinatorial screening in which building blocks, instead of being linked together chemically, are placed on the surface of nanoparticles. Two- or three-dimensional structures form on the surface of these particles through the close approach of different building blocks, with sufficient flexibility to be able to adapt and interact with putative binding sites in biological systems. The particles assemble without the need for formation of chemical bonds, so libraries comprised of many structures can be prepared rapidly, with large quantities of material available for testing. Screening methods can include solid and solution-phase binding assays, or tissue culture models, for example looking for structures which can change the behaviour of cells in a disease-modifying manner.
authors.affiliation: Proxima Concepts Limited, c/o London Bioscience Innovation Centre, 2 Royal College Street, London NW1 0NH, UK. rogernew@proximaconcepts.com
authors.forename: Roger
authors.initials: R
authors.lastname: New
authors.forename: Gurpal S
authors.initials: GS
authors.lastname: Bansal
authors.forename: Michael
authors.initials: M
authors.lastname: Bogus
authors.forename: Kasia
authors.initials: K
authors.lastname: Zajkowska
authors.forename: Steffen
authors.initials: S
authors.lastname: Rickelt
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001414:Bacitracin / Q000737:chemistry; D002460:Cell Line; D020650:Combinatorial Chemistry Techniques; D055808:Drug Discovery; D031541:Fluorescence Resonance Energy Transfer; D007074:Immunoglobulin G / Q000737:chemistry; D051379:Mice; D008823:Micelles; D009113:Muramidase / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding; D054852:Small Molecule Libraries / Q000138:chemical synthesis; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000596:Amino Acids; D007074:Immunoglobulin G; D008823:Micelles; D009842:Oligopeptides; D054852:Small Molecule Libraries; D013501:Surface-Active Agents; D014409:Tumor Necrosis Factor-alpha; D001414:Bacitracin; D009113:Muramidase