title: Association of human papillomavirus genomic sequences by polymerase chain reaction in gastric carcinomas in Brazil.
abstract: OBJECTIVE
  To investigate the prevalence of human papillomavirus (HPV) in patients with gastric cancer and compare with a control group.
  
  
  METHODS
  Forty paraffin samples of gastric cancer and 40 endoscopic normal mucosa and peripheral blood controls were subjected to examination by polymerase chain reaction for the L1 gene of HPV. The extracted DNA was amplified in 2 reaction systems using 2 pairs of primers: MY09/MY11 and GP+5/GP+6. We used Milli-Q water as negative control and a mixture of 1 microL of human blood plus 0.25 microL of plasmid pBR322 (HPV-16) as positive control.
  
  
  RESULTS
  HPV was found in 4 patients with gastric cancer and 10 patients without cancer.
  
  
  CONCLUSIONS
  There was no statistically significant difference between the 2 samples (p = 0.077).
authors.affiliation: Department of Surgery, Clinics Hospital-Federal University of Pernambuco, Prof. Moraes Rego Avenue, 1235 University City, Pernambuco, Brazil. luna.anaclaudia@gmail.com
authors.forename: Ana Claudia L
authors.initials: AC
authors.lastname: Cândido
authors.forename: José Luiz
authors.initials: JL
authors.lastname: de Lima Filho
authors.forename: Danyelly Bruneska G
authors.initials: DB
authors.lastname: Martins
authors.forename: Chirleanny Maciel
authors.initials: CM
authors.lastname: Mendes
authors.forename: Jeymesson Raphael C
authors.initials: JR
authors.lastname: Vieira
authors.forename: Alvaro Antônio B
authors.initials: AA
authors.lastname: Ferraz
mesh_terms: D001938:Brazil; D036022:Capsid Proteins / Q000032:analysis* / Q000235:genetics; D003430:Cross-Sectional Studies; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000032:analysis* / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000821:virology*
chemical_list: D036022:Capsid Proteins; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral