title: Selective enhancement of carbohydrate ion abundances by diamond nanoparticles for mass spectrometric analysis.
abstract: Diamond nanoparticles (DNPs) were incorporated into matrix-assisted laser desorption/ionization (MALDI) samples to enhance the sensitivity of the mass spectrometer to carbohydrates. The DNPs optimize the MALDI sample morphology and thermalize the samples for thermally labile compounds because they have a high thermal conductivity, a low extinction coefficient in UV-vis spectral range, and stable chemical properties. The best enhancement effect was achieved when matrix, DNP, and carbohydrate solutions were deposited and vacuum-dried consecutively to form a trilayer sample morphology. It allows the direct identification of underivatized carbohydrates mixed with equal amount of proteins because no increase in the ion abundance of proteins was achieved. For dextran with an average molecular weight of 1500, the trilayer method typically improves the sensitivity by 79- and 7-fold in comparison to the conventional dried-droplet and thin-layer methods, respectively.
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC.
authors.forename: Chieh-Lin
authors.initials: CL
authors.lastname: Wu
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Wang
authors.forename: Yin-Hung
authors.initials: YH
authors.lastname: Lai
authors.forename: Hsun
authors.initials: H
authors.lastname: Lee
authors.forename: Jia-Der
authors.initials: JD
authors.lastname: Lin
authors.forename: Yuan Tseh
authors.initials: YT
authors.lastname: Lee
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Wang
mesh_terms: D003911:Dextrans / Q000032:analysis*; D018130:Diamond / Q000737:chemistry*; D006358:Hot Temperature; D007477:Ions; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D059629:Signal-To-Noise Ratio; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D003911:Dextrans; D007477:Ions; D011506:Proteins; D018130:Diamond