title: Cell culture purity issues and DFAT cells.
abstract: Dedifferentiation of mature adipocytes, in vitro, has been pursued/documented for over forty years. The subsequent progeny cells are named dedifferentiated adipocyte-derived progeny cells (DFAT cells). DFAT cells are proliferative and likely to possess mutilineage potential. As a consequence, DFAT cells and their progeny/daughter cells may be useful as a potential tool for various aspects of tissue engineering and as potential vectors for the alleviation of several disease states. Publications in this area have been increasing annually, but the purity of the initial culture of mature adipocytes has seldom been documented. Consequently, it is not always clear whether DFAT cells are derived from dedifferentiated mature (lipid filled) adipocytes or from contaminating cells that reside in an impure culture.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China.
authors.forename: Shengjuan
authors.initials: S
authors.lastname: Wei
authors.forename: Werner G
authors.initials: WG
authors.lastname: Bergen
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Hausman
authors.forename: Linsen
authors.initials: L
authors.lastname: Zan
authors.forename: Michael V
authors.initials: MV
authors.lastname: Dodson
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D054337:Cell Dedifferentiation; D002469:Cell Separation / Q000379:methods / Q000592:standards*; D006801:Humans; D061251:Primary Cell Culture; D023822:Tissue Engineering