title: Significant decrease of broth viscosity and glucose consumption in erythromycin fermentation by dynamic regulation of ammonium sulfate and phosphate.
abstract: In this study, the effects of nitrogen sources on broth viscosity and glucose consumption in erythromycin fermentation were investigated. By controlling ammonium sulfate concentration, broth viscosity and glucose consumption were decreased by 18.2% and 61.6%, respectively, whereas erythromycin biosynthesis was little affected. Furthermore, erythromycin A production was increased by 8.7% still with characteristics of low broth viscosity and glucose consumption through the rational regulations of phosphate salt, soybean meal and ammonium sulfate. It was found that ammonium sulfate could effectively control proteinase activity, which was correlated with the utilization of soybean meal as well as cell growth. The pollets formation contributed much to the decrease of broth viscosity. The accumulation of extracellular propionate and succinate under the new regulation strategy indicated that higher propanol consumption might increase the concentration of methylmalonyl-CoA and propionyl-CoA and thus could increase the flux leading to erythromycin A.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Zejian
authors.initials: Z
authors.lastname: Wang
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Siliang
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yu
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D004917:Erythromycin / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D025282:Mycelium / Q000187:drug effects; D009584:Nitrogen / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology*; D016930:Saccharopolyspora / Q000187:drug effects / Q000378:metabolism; D012995:Solubility; D013025:Glycine max / Q000737:chemistry; D014783:Viscosity / Q000187:drug effects
chemical_list: D000596:Amino Acids; D003470:Culture Media; D010710:Phosphates; D004917:Erythromycin; D005947:Glucose; D009584:Nitrogen; D010100:Oxygen; D000645:Ammonium Sulfate