title: Goose Toll-like receptor 7 (TLR7), myeloid differentiation factor 88 (MyD88) and antiviral molecules involved in anti-H5N1 highly pathogenic avian influenza virus response.
abstract: In mammals, Toll-like receptor 7 (TLR7) is an important membrane-bound receptor triggered by antiviral compounds and single-stranded RNA. It is implicated in the immune response to viruses such as influenza virus. It was not known whether geese, a natural host for avian influenza viruses, possess a homologue of mammalian TLR7 for recognizing avian influenza virus. In this study, we cloned the full-length of goose TLR7 and partial sequences of its adaptor protein, myeloid differentiation factor 88 (MyD88), some antiviral molecules such as RNA-dependent protein kinase (PKR) and 2',5'-oligoadenylate synthetase (OAS). Goose TLR7 has a protein secondary structure identical to that of mammals, consisting of several leucine-rich domains, a transmembrane domain, and Toll/interleukin-1 receptor domain. To further understand whether the MyD88-dependent pathway of TLR7 is involved in the antiviral innate immune response against highly pathogenic avian influenza virus (HPAIV) infection in geese, we inoculated geese with an H5N1 HPAIV isolated from ducks in 2004. The virus, A/Duck/Guangdong/212/2004, replicated in various tissues resulting in 40% mortality. Quantitative real-time PCR analysis showed upregulation of mRNA transcripts for TLR7, MyD88, PKR and OAS in the lungs of geese at 1, 2 and 3 days post-inoculation. Therefore, the MyD88-dependent pathway of TLR7 was involved in the early stage of antiviral innate immune response in geese during H5N1 HPAIV infection.
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, PR China.
authors.forename: Liangmeng
authors.initials: L
authors.lastname: Wei
authors.forename: Peirong
authors.initials: P
authors.lastname: Jiao
authors.forename: Runyu
authors.initials: R
authors.lastname: Yuan
authors.forename: Yafen
authors.initials: Y
authors.lastname: Song
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cui
authors.forename: Xuchen
authors.initials: X
authors.lastname: Guo
authors.forename: Bofang
authors.initials: B
authors.lastname: Zheng
authors.forename: Weixin
authors.initials: W
authors.lastname: Jia
authors.forename: Wenbao
authors.initials: W
authors.lastname: Qi
authors.forename: Tao
authors.initials: T
authors.lastname: Ren
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D005777:Geese / Q000276:immunology*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000276:immunology*; D008168:Lung / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000502:physiology*; D051199:Toll-Like Receptor 7 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication; D019892:eIF-2 Kinase / Q000502:physiology
chemical_list: D053594:Myeloid Differentiation Factor 88; D051199:Toll-Like Receptor 7; D019892:eIF-2 Kinase; D015088:2',5'-Oligoadenylate Synthetase