title: In vitro inactivation of porcine reproductive and respiratory syndrome virus and pseudorabies virus by slightly acidic electrolyzed water.
abstract: Slightly acidic electrolyzed water (SAEW, pH 5.0-6.5) is a novel disinfectant with environmentally friendly broad spectrum microbial decontamination properties which could have significant utility on farm. Two of the most important pathogenic viruses in pigs are porcine reproductive and respiratory syndrome virus (PRRSV) and pseudorabies virus (PRV). The aim of this study was to evaluate the viricidal effectiveness of SAEW against PRRSV and PRV in vitro under different available chlorine concentrations (ACCs, 30, 50 and 70 mg/L), treatment times (5, 10 and 15 min) and temperatures (4, 20, 40 and 60°C), respectively. SAEW had a strong viricidal activity against both PRRSV and PRV. This activity increased with increasing ACC, treatment time and temperature. PRRSV and PRV titres of 7.0 log(10)TCID(50)/mL and 5.9 log(10)TCID(50)/mL, respectively, were completely inactivated by SAEW at an ACC of ≥ 50 mg/L for 10 min even though SAEW had no negative effect on the host cells. SAEW thus shows promise as a disinfectant for use on pig farms to reduce the spread of both PRRSV and PRV, and to limit the morbidity associated with those viruses.
authors.affiliation: Key Laboratory of Bio-environmental Engineering, Ministry of Agriculture, China Agricultural University, P.O. Box 67, Beijing 100083, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Hao
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Shen
authors.forename: Jinliang
authors.initials: J
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Baoming
authors.initials: B
authors.lastname: Li
authors.forename: Chaoyuan
authors.initials: C
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D000818:Animals; D002460:Cell Line; D002713:Chlorine / Q000737:chemistry / Q000494:pharmacology*; D006224:Cricetinae; D004202:Disinfectants / Q000737:chemistry* / Q000494:pharmacology*; D004572:Electrolysis; D011558:Herpesvirus 1, Suid / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007928:Lethal Dose 50; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004202:Disinfectants; D014867:Water; D002713:Chlorine
keywords: Disinfection; PRRSV; PRV; Slightly acidic electrolyzed water; Swine