title: Biomimetic mineralization of novel silane crosslinked collagen.
abstract: Studies on the mechanisms of mineralization of connective tissues, have gained momentum in the recent past. In the present study, Biomimetic mineralization of modified fish scale collagen in vitro is reported. The fish scale collagen was crosslinked with 3-Aminopropyl triethoxysilane and the crosslinked collagen (FCSi) was characterized using conventional methods. The biomimetic mineralization capacity of FCSi was evaluated in SBF solution for 7 days. Formation of hydroxyapatite crystals on the matrix in vitro has been confirmed by FTIR, XRD and SEM-EDAX techniques. The FCSi may be used as a scaffold in bone tissue engineering and as an osteoinductive material in experimental animal models before applying clinically.
authors.affiliation: Bioproducts Lab, Central Leather Research Institute, Chennai 20, India.
authors.forename: R Narasimha
authors.initials: RN
authors.lastname: Raghavan
authors.forename: T
authors.initials: T
authors.lastname: Muthukumar
authors.forename: N
authors.initials: N
authors.lastname: Somanathan
authors.forename: T P
authors.initials: TP
authors.lastname: Sastry
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005399:Fishes; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D003432:Cross-Linking Reagents; D008903:Minerals; D011437:Propylamines; D012821:Silanes; D003094:Collagen; C477625:amino-propyl-triethoxysilane