title: Growth and survival of cells in biosynthetic poly vinyl alcohol-alginate IPN hydrogels for cardiac applications.
abstract: Biosynthetic hydrogels of poly vinyl alcohol-calcium alginate were prepared as semi (semi-IPN hydrogel, PAH) and full interpenetrating polymeric network (IPN hydrogel, PAHG) for tissue engineering of cardiac tissue. The biological response of these hydrogels was studied. The IPN hydrogel exhibits amphiphilic nature and moderate equilibrium water content. The IPN hydrogel inherits the water as structured one along with more free water. The IPN hydrogel has adequate mechanical strength and fatigue life and stability in physiological media over the semi-IPN hydrogel. The structured water along with more free water in IPN hydrogel promotes blood compatibility, cell adhesion and proliferation and maintains three dimensional growths of L929 fibroblast and H9C2 cardiomyoblasts. IPN hydrogel maintain long term cell viability and infiltration with exchange of nutrients in the interstices of the hydrogel. The IPN hydrogel, PAHG is a promising material for engineering cardiac tissue.
authors.affiliation: Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Science Division, Biomedical Technology Wing, Thiruvananthapuram 695012, Kerala, India.
authors.forename: Finosh
authors.initials: F
authors.lastname: Gnanaprakasam Thankam
authors.forename: Jayabalan
authors.initials: J
authors.lastname: Muthu
authors.forename: Vandana
authors.initials: V
authors.lastname: Sankar
authors.forename: Raghu
authors.initials: R
authors.lastname: Kozhiparambil Gopal
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001673:Biodegradation, Environmental; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005612:Freeze Drying; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol