title: Isolation and identification of an iopromide-degrading strain and its application in an A2/O system.
abstract: An iopromide (IOPr)-degrading bacterium was isolated from activated sludge of a wastewater treatment plant in Shanghai. Based on its morphology, physiological-biochemical characteristics and a phylogenetic analysis of its 16S rRNA sequence, the bacterium was identified and named as Pseudomonas sp. I-24. The optimum condition for degrading IOPr was at 30°C and pH 7.0. After 5 days, strain I-24 could degrade 30 mg/L IOPr by 99% in a basal salts medium with a 5% (V/V) inoculum and 200 mg/L starch as the primary substrate. When applied to an Anaerobic-Anoxic/Aerobic (A2/O) process, with the coexistence of other bacteria, the strain I-24 got lower (61.3%) IOPr removal, but in two A2/O systems (with and without I-24 inoculation), the CODcr removal were both approximately 95%. The trial dosed with strain I-24 showed better IOPr removal than the un-dosed one. I-24 sustained its abundance in the A2/O system during the experiment.
authors.affiliation: School of Environmental Science and Engineering, Dong Hua University, Shanghai 201620, China. liuyanan@gmail.com
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.forename: Jinlong
authors.initials: J
authors.lastname: Hu
authors.forename: Bingjie
authors.initials: B
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Pin
authors.initials: P
authors.lastname: Gao
authors.forename: Kaiying
authors.initials: K
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Xue
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Ognier
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D015169:Colony Count, Microbial; D058645:Denaturing Gradient Gel Electrophoresis; D007472:Iohexol / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification
chemical_list: D007472:Iohexol; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater; C038192:iopromide