title: Dissimilar characteristics of umbilical cord mesenchymal stem cells from donors of different ages.
abstract: Increasing attention is being placed on the use of adult stem cells due to their potential therapeutic applications for unmet medical needs. Among all adult stem cell types, umbilical cord mesenchymal stem cells (UCMSCs) can be collected easily and does not harm newborns and the mothers. The excellent pluripotency and proliferation potential of UCMSCs has been previously demonstrated by many groups. However, little is known about the different characteristics of these cells due to diversity of donors (e.g., the mothers' age). Regarding this, we examined the effect of mothers' age-related variation on the basic properties (proliferation, colony forming and differentiation potential) of UCMSCs in vitro. The data evidenced that elder mother results in reduction of both proliferative and colony forming capacity of UCMSCs, and impacts proliferative potential more significantly. Data also evidenced that a decline in osteogenic potential, but an increase in adipogenic potential of UCMSCs from the elder donors. Additionally, no phenotypic differences were observed by flow cytometry analysis using a panel of 6 main surface antigen markers. We observed no differences in karyotyping and both UCMSCs populations exhibited diploid cells without chromosomal aberrations. The results of our study are the basis for banking UCMSCs, which may be labeled with donors' information for different experiments, and the mother age-related differences in characteristics of UCMSCs should be taken into account when these cells are considered for further practical applications.
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing, 100853, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Huang
authors.forename: Changjiang
authors.initials: C
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Siming
authors.initials: S
authors.lastname: Yang
authors.forename: Kui
authors.initials: K
authors.lastname: Ma
authors.forename: Xu
authors.initials: X
authors.lastname: Wu
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D000328:Adult; D000367:Age Factors; D000375:Aging / Q000502:physiology*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002999:Clone Cells; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D014019:Tissue Donors; D014470:Umbilical Cord / Q000166:cytology*; D055815:Young Adult