title: [Isolation and identification of Streptococcus mutans strains with different genotype from clinical samples].
abstract: OBJECTIVE
  To identify Streptococcus mutans (S. mutans) strains from clinical samples.
  
  
  METHODS
  Plaque samples from caries-active and caries-free sites on enamel surfaces were obtained and cultivated for S. mutans isolation. Morphology, biochemistry, automatic microorganism analysis system and polymerase chain reaction using primers homologous to surface protein antigen I/II (spaP), glucosyltransferase B (gtfB) and dextranase (dexA) were used to identify S. mutans. Genotype of isolated S. mutans was determined by arbitrarily primed polymerase chain reaction.
  
  
  RESULTS
  Forty-six strains of S. mutans were obtained from the 32 subjects and were identified as S. mutans by biochemistry, automatic microorganism analysis system and polymerase chain reaction. Five identical genotypes were found by arbitrarily primed polymerase chain reaction.
  
  
  CONCLUSIONS
  Forty-one strains of S. mutans with different genotype were obtained from clinical samples.
authors.affiliation: Dept. of Stomatology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Wang
authors.forename: Donghua
authors.initials: D
authors.lastname: Su
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Liu
authors.forename: Bingfeng
authors.initials: B
authors.lastname: Chu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Yanping
authors.initials: Y
authors.lastname: Luo
authors.forename: Jiyong
authors.initials: J
authors.lastname: Yang
authors.forename: Ding
authors.initials: D
authors.lastname: Hongmei
authors.forename: Zhao
authors.initials: Z
authors.lastname: Qiang
authors.forename: Deng
authors.initials: D
authors.lastname: Bin
authors.forename: Xi
authors.initials: X
authors.lastname: Qing
authors.forename: Xu
authors.initials: X
authors.lastname: Juan
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D003731:Dental Caries; D003773:Dental Plaque; D005838:Genotype; D005964:Glucosyltransferases; D006801:Humans; D016133:Polymerase Chain Reaction; D013295:Streptococcus mutans
chemical_list: C036655:1,3-alpha-D-glucan synthase; D005964:Glucosyltransferases