title: In-vitro and in-vivo analysis of the production of the Bordetella type three secretion system effector A in Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica.
abstract: Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica are three closely related pathogens. They all possess the gene coding for the Bordetella type three secretion system effector A (bteA) toxin that became a focus of interest since it was demonstrated that B. pertussis Japanese non-vaccine-type isolates produce BteA unlike vaccine-type isolates. We thus explored the in-vitro production of BteA in B. pertussis isolates collected in France during periods of different vaccine policy as well as in B. parapertussis and B. bronchiseptica isolates. We also analyzed the in-vivo induction of anti-BteA antibodies after infection with different isolates of the three species. We produced a recombinant His6-tagged BteA (rBteA) protein. Specific rBteA polyclonal serum was prepared which enabled us to screen Bordetella isolates for in-vitro BteA production: 99.0% (293/296) of tested B. pertussis isolates, including French vaccine strains, and 97.5% (79/81) of B. bronchiseptica isolates produced BteA in-vitro but only the latter was capable of inducing an in-vivo immune response. No in-vitro or in-vivo production of BteA was detected by any of the B. parapertussis isolates tested.
authors.affiliation: Institut Pasteur, 25-28 rue du Dr Roux, 75015 Paris, France; CNRS-URA3012, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Hegerle
authors.forename: Lamya
authors.initials: L
authors.lastname: Rayat
authors.forename: Grégory
authors.initials: G
authors.lastname: Dore
authors.forename: Nora
authors.initials: N
authors.lastname: Zidane
authors.forename: Hugues
authors.initials: H
authors.lastname: Bedouelle
authors.forename: Nicole
authors.initials: N
authors.lastname: Guiso
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001885:Bordetella Infections / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000302:isolation & purification / Q000472:pathogenicity*; D042483:Bordetella parapertussis / Q000302:isolation & purification / Q000472:pathogenicity*; D001886:Bordetella pertussis / Q000302:isolation & purification / Q000472:pathogenicity*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D005602:France; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000096:biosynthesis*
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D037521:Virulence Factors