title: Graphene bridged enzyme electrodes for glucose biosensing application.
abstract: The fabrication of glucose oxidase (GOx) enzyme electrodes with controlled alternate enzyme and graphene layers is described. GOx was first modified with pyrene functionalities in order to be self-assembled onto a graphene basal plane via non-covalent π-π stacking interaction. Fluorescence spectroscopy analysis revealed that about 5.4 pyrene functional groups were attached to each GOx and the pyrene functionalized GOx retained more than 76% of the biocatalytical activity compared with the native enzyme. Via alternate layer-by-layer self-assembly of graphene and pyrene functionalized GOx, mono- and multi-layered enzyme electrodes with controlled biocatalytical activity can be easily fabricated. The biocatalytical activity of the as-prepared enzyme electrodes increased with increasing graphene and GOx layers and increased insignificantly when the layers reached four. Such multi-layered enzyme electrodes with controlled nanostructure exhibited reliable application in human serum samples analysis with high detection sensitivity, good stability and repeatability. A broad linear detection limit of 0.2 to 40 mM was obtained.
authors.affiliation: College of Chemistry, Chemical and Environmental Engineering, Laboratory of Fiber Materials and Modern Textile, Qingdao University, Qingdao 266071, PR China. jliu@qdu.edu.cn
authors.forename: Jingquan
authors.initials: J
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Kong
authors.forename: Aihua
authors.initials: A
authors.lastname: Li
authors.forename: Xiong
authors.initials: X
authors.lastname: Luo
authors.forename: Liang
authors.initials: L
authors.lastname: Cui
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Shengyu
authors.initials: S
authors.lastname: Feng
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004867:Equipment Design; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D006108:Graphite; D005949:Glucose Oxidase