title: [Pharmacokinetic study on three main ingredients of refined coronary cataplasm].
abstract: OBJECTIVE
  To establish a LC-MS/MS method for determining the concentration of tanshinone IIA, salvianolic acid B and paeoniflorin of refined coronary cataplasm in rabbit plasma, in order to determine the concentration of the three main ingredients in blood after transdermal administration and calculate their pharmacokinetic parameters.
  
  
  METHODS
  Rabbits were given refined coronary cataplasm on the basis of 15 g x kg(-1) by transdermal administration to detect the plasma concentration of the three main ingredients using LC-MS/MS. Winnonlin software was used to calculate their major pharmacokinetic parameters.
  
  
  RESULTS
  Tanshinone IIA, salvianolic acid B and paeoniflorin showed good linearity (r>0.999) at 1-100, 50-1 000, 10-1 000 microg x L(-1) respectively in plasma, with average recovery rate of 96.57%, 91.90%, 95.93%, respectively. The RSD within day were less than 15%. After transdermal administration of refined coronary cataplasm in rabbits, the main pharmacokinetic parameters of tanshinone IIA, salvianolic acid B or paeoniflorin were as follows: Cmax (20.85 +/- 12.68), (636.25 +/- 386.91), (787.80 +/- 395.64) microg x L(-1); Tmax (0.49 +/- 0.28), (0.44 +/- 0.27), (0.46 +/- 0.30) h.
  
  
  CONCLUSIONS
  The LC-MS/MS method is highly selective and sensitive to determine the concentration of samples in rabbit plasma. The pharmacokinetic characteristics of tanshinone IIA, salvianolic acid B and paeoniflorin are suitable to assess the percutaneous absorption of refined coronary cataplasm.
authors.affiliation: Capital Medical University, Beijing 100069, China. houri527@163.com
authors.forename: Rui
authors.initials: R
authors.lastname: Hou
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Mao-Bo
authors.initials: MB
authors.lastname: Du
authors.forename: Ri-Xin
authors.initials: RX
authors.lastname: Liang
authors.forename: Shu-Zhi
authors.initials: SZ
authors.lastname: Liu
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Wang
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D045784:Abietanes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D001565:Benzoates / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001572:Benzofurans / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001952:Bridged-Ring Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D003327:Coronary Disease / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005960:Glucosides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D039821:Monoterpenes; D011817:Rabbits; D053719:Tandem Mass Spectrometry / Q000379:methods*; D006027:Glycosides; D053630:Depsides
chemical_list: D045784:Abietanes; D001565:Benzoates; D001572:Benzofurans; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C021751:tanshinone; C015423:peoniflorin; D006027:Glycosides; C076944:salvianolic acid B; D053630:Depsides