title: ZnS nanoparticles electrodeposited onto ITO electrode as a platform for fabrication of enzyme-based biosensors of glucose.
abstract: The electrochemical and photoelectrochemical biosensors based on glucose oxidase (GOD) and ZnS nanoparticles modified indium tin oxide (ITO) electrode were investigated. The ZnS nanoparticles were electrodeposited directly on the surface of ITO electrode. The enzyme was immobilized on ZnS/ITO electrode surface by sol-gel method to fabricate glucose biosensor. GOD could electrocatalyze the reduction of dissolved oxygen, which resulted in a great increase of the reduction peak current. The reduction peak current decreased linearly with the addition of glucose, which could be used for glucose detection. Moreover, ZnS nanoparticles deposited on ITO electrode surface showed good photocurrent response under illumination. A photoelectrochemical biosensor for the detection of glucose was also developed by monitoring the decreases in the cathodic peak photocurrent. The results indicated that ZnS nanoparticles deposited on ITO substrate were a good candidate material for the immobilization of enzyme in glucose biosensor construction.
authors.affiliation: College of Chemistry, Chemical Engineering and Material Science, Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Du
authors.forename: Xiuping
authors.initials: X
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: Junwei
authors.initials: J
authors.lastname: Di
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D002138:Calibration; D002384:Catalysis; D004560:Electricity; D004566:Electrodes; D004595:Electroplating / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D044367:Phase Transition; D015203:Reproducibility of Results; D012996:Solutions; D013440:Sulfides / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry*; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D002021:Buffers; D012996:Solutions; D013440:Sulfides; D017971:Tin Compounds; D017967:Zinc Compounds; C109984:indium tin oxide; D005949:Glucose Oxidase; D005947:Glucose; C031238:zinc sulfide