title: Discovery of cancer biomarkers using antibody arrays.
abstract: BACKGROUND
  The cancer biomarkers field is being enriched by molecular profiling obtained by high-throughput approaches. In the context of other proteomic technologies, targeted antibody arrays are contributing strongly to the identification of protein cancer biomarker candidates.
  
  
  OBJECTIVE
  To describe innovative strategies multiplexing protein measurements of protein extracts of cultured cells, tissue and body fluids using antibody arrays that, combined with appropriate validation approaches, are enabling the discovery of cancer-associated biomarkers.
  
  
  METHODS
  The strategies and cancer biomarker candidates identified using antibody arrays reported so far that may assist in the diagnosis, surveillance and prognosis, and potentially for predictive and therapeutic purposes for patients affected with solid and hematological neoplasias.
  
  
  CONCLUSIONS
  Owing to their versatility, novel technological and experimental design implementations are broadening the applications of antibody arrays also for biomarker discovery. However, the cancer biomarker candidates delivered so far using this technology are still at an early developmental phase, requiring validation with many specimens focusing on specific clinical end points.
authors.affiliation: Group Leader Spanish National Cancer Research Center, Tumor Markers Group, 308A, Melchor Fernandez Almagro 3, E-28029 Madrid, Spain +34 91 7328053 ; +34 91 224 6972 ; mscarbayo@cnio.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Sanchez-Carbayo