title: Morphology and structural properties of high-amylose rice starch residues hydrolysed by amyloglucosidase.
abstract: High-amylose starches are attracting considerable attention because of their potential health benefits and industrial uses. Enzyme hydrolysis of starch is involved in many biological and industrial processes. In this paper, starches were isolated from high-amylose transgenic rice (TRS) and its wild type rice, Te-qing (TQ). The morphological and structural changes of starch residues following Aspergillus niger amyloglucosidase (AAG) hydrolysis were investigated. AAG hydrolysed TQ starch from the granule surface, and TRS starch from the granule interior. During AAG hydrolysis, the content of amorphous structure increased, the contents of ordered structure and single helix decreased, and gelatinisation enthalpy decreased in TQ and TRS starch residues. The A-type polymorph of TRS C-type starch was hydrolysed faster than the B-type polymorph. The short-range ordered structure and B-type polymorph in the peripheral region of the subgranule and the surrounding band of TRS starch increased the resistance of TRS starch to AAG hydrolysis.
authors.affiliation: Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Man
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.forename: Changquan
authors.initials: C
authors.lastname: Zhang
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
authors.forename: Youping
authors.initials: Y
authors.lastname: Wang
authors.forename: Minghong
authors.initials: M
authors.lastname: Gu
authors.forename: Qiaoquan
authors.initials: Q
authors.lastname: Liu
authors.forename: Cunxu
authors.initials: C
authors.lastname: Wei
mesh_terms: D000688:Amylose / Q000032:analysis*; D001234:Aspergillus niger / Q000201:enzymology*; D005656:Fungal Proteins / Q000737:chemistry*; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D006868:Hydrolysis; D015394:Molecular Structure; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D013213:Starch / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D013213:Starch; D000688:Amylose; D005087:Glucan 1,4-alpha-Glucosidase