title: Biocontrol Efficacy Among Strains of Pochonia chlamydosporia Obtained from a Root-Knot Nematode Suppressive Soil.
abstract: Three Pochonia chlamydosporia var. chlamydosporia strains were isolated from a Meloidogyne incognita-suppressive soil, and then genetically characterized with multiple Pochonia-selective typing methods based on analysis of ß-tubulin, rRNA internal transcribed spacer (ITS), rRNA small subunit (SSU), and enterobacterial repetitive intergenic consensus (ERIC) PCR. All strains exhibited different patterns with the ERIC analysis. Strains 1 and 4 were similar with PCR analysis of ß-tubulin and ITS. The strains' potential as biological control agents against root-knot nematodes were examined in greenhouse trials. All three P. chlamydosporia strains significantly reduced the numbers of nematode egg masses. When chlamydospores were used as inoculum, strain 4 reduced egg numbers on tomato roots by almost 50%, and showed effects on the numbers of J2 and on nematode-caused root-galling. A newly developed SSU-based PCR analysis differentiated strain 4 from the others, and could therefore potentially be used as a screening tool for identifying other effective biocontrol strains of P. chlamydosporia var. chlamydosporia.
authors.affiliation: Departments of Plant Pathology and Microbiology, and.
authors.forename: Jiue-In
authors.initials: JI
authors.lastname: Yang
authors.forename: Angelo
authors.initials: A
authors.lastname: Loffredo
authors.forename: James
authors.initials: J
authors.lastname: Borneman
authors.forename: J Ole
authors.initials: JO
authors.lastname: Becker
keywords: Meloidogyne incognita; Pochonia chlamydosporia; Southern root-knot nematode; biological control; suppressive soil