title: New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load.
abstract: BACKGROUND
  Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out to be a promising tool for the development of more sensitive diagnostic methods. In our previous investigations, the use of crude antigens led to false-positive results. Recently, focus has been given to highly purified Schistosoma mansoni antigens, especially to circulating antigens.
  
  
  METHODS
  Thus, our main goal was to test different types of circulating cathodic antigen glycoprotein (CCA), as "crude antigen," the protein chain of recombinant CCA and two individual peptides. These schistosome proteins/peptides were tested in a new diagnostic method employing immunomagnetic separation based on the improvement of antigen-antibody binding.
  
  
  RESULTS
  Use of recombinant CCA as a diagnostic antigen allowed us to develop a diagnostic assay with high sensitivity and specificity with no false-negative results. Interestingly, the "crude antigen" worked as a good marker for control of cure after praziquantel treatment.
  
  
  CONCLUSIONS
  Our new diagnostic method was superior to enzyme-linked immunosorbent assay in diagnosing low endemicity patients.
authors.affiliation: Schistosomiasis Laboratory, Rene Rachou Research Center, Oswaldo Cruz Foundation (Fiocruz), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Rafaella
authors.initials: R
authors.lastname: Grenfell
authors.forename: Donald A
authors.initials: DA
authors.lastname: Harn
authors.forename: Smanla
authors.initials: S
authors.lastname: Tundup
authors.forename: Akram
authors.initials: A
authors.lastname: Da'dara
authors.forename: Liliane
authors.initials: L
authors.lastname: Siqueira
authors.forename: Paulo Marcos Zech
authors.initials: PM
authors.lastname: Coelho
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth; D002648:Child; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D059208:Parasite Load; D010274:Parasitology / Q000379:methods*; D011446:Prospective Studies; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000469:parasitology; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G