title: Transcriptional regulation of the ADP-glucose pyrophosphorylase isoforms in the leaf and the stem under long and short photoperiod in lentil.
abstract: ADP-glucose pyrophosphorylase (AGPase) is a key enzyme in plant starch biosynthesis. It contains large (LS) and small (SS) subunits encoded by two different genes. In this study, we explored the transcriptional regulation of both the LS and SS subunits of AGPase in stem and leaf under different photoperiods length in lentil. To this end, we first isolated and characterized different isoforms of the LS and SS of lentil AGPase and then we performed quantitative real time PCR (qPCR) to see the effect of photoperiod length on the transcription of the AGPase isforms under the different photoperiod regimes in lentil. Analysis of the qPCR results revealed that the transcription of different isoforms of the LSs and the SSs of lentil AGPase are differentially regulated when photoperiod shifted from long-day to short-day in stem and leaves. While transcript levels of LS1 and SS2 in leaf significantly decreased, overall transcript levels of SS1 increased in short-day regime. Our results indicated that day length affects the transcription of lentil AGPase isoforms differentially in stems and leaves most likely to supply carbon from the stem to other tissues to regulate carbon metabolism under short-day conditions.
authors.affiliation: Koc University, Department of Chemical and Biological Engineering, Rumeli Feneri Yolu, 34450 Sariyer, Istanbul, Turkey.
authors.forename: Ayse Bengisu
authors.initials: AB
authors.lastname: Seferoglu
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Baris
authors.forename: Hande
authors.initials: H
authors.lastname: Morgil
authors.forename: Isil
authors.initials: I
authors.lastname: Tulum
authors.forename: Sule
authors.initials: S
authors.lastname: Ozdas
authors.forename: Gul
authors.initials: G
authors.lastname: Cevahir
authors.forename: Ibrahim Halil
authors.initials: IH
authors.lastname: Kavakli
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007527:Isoenzymes; D007700:Kinetics; D027806:Lens Plant / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017440:Photoperiod; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D013213:Starch / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D013213:Starch; D051599:Glucose-1-Phosphate Adenylyltransferase