title: Study of the impact of uterine artery embolization (UAE) on endometrial microvessel density (MVD) and angiogenesis.
abstract: OBJECTIVE
  To investigate the influence of uterine artery embolization (UAE) on endometrial microvessel density (MVD) and angiogenesis.
  
  
  METHODS
  Sixty female guinea pigs were divided into two groups, the control group (n = 15) and the UAE treatment group (n = 45). In the UAE group, tris-acryl gelatin microspheres were used to generate embolization. Animals were further divided into three subgroups, A1, A2, and A3 (n = 15 for each subgroup), with uterine specimens collected at 7-15, 16-30, and 31-45 days after UAE, respectively. Immunostaining for factor VIII and CD105 was performed to identify total endometrial MVD (MVDFVIII) and CD105-positive angiogenesis (MVDCD105) at the indicated time points after UAE.
  
  
  RESULTS
  Quantitative analysis revealed that MVDFVIII significantly decreased in the A1 (11.40 ± 2.76, p < 0.05) and A2 (15.37 ± 3.06, p < 0.05) groups compared to the control group (19.40 ± 2.50), and was restored to normal in the A3 group (18.77 ± 2.69). UAE caused a temporal up-regulation of MVDCD105-positive angiogenesis in the A1 group (9.33 ± 2.37, p < 0.05) and the A2 group (11.63 ± 1.56, p < 0.05) compared to the control group (7.12 ± 1.67), and the MVDCD105 value returned to normal in the A3 group (8.07 ± 1.97).
  
  
  CONCLUSIONS
  UAE caused a temporal decrease in endometrial MVD that reversed over time as a result of the increase of CD105-positive angiogenesis. Although the UAE-induced reduction of endometrial MVD was reversible, its long-term effect on endometrial receptivity still needs further study.
authors.affiliation: Department of Interventional Radiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Tan
authors.forename: Xianhong
authors.initials: X
authors.lastname: Xiang
authors.forename: Wenbo
authors.initials: W
authors.lastname: Guo
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Jianyong
authors.initials: J
authors.lastname: Yang
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D000704:Analysis of Variance; D000792:Angiography / Q000379:methods; D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D004717:Endometrium / Q000000981:diagnostic imaging / Q000473:pathology*; D005260:Female; D005780:Gelatin / Q000494:pharmacology*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D055806:Microvessels / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000209:etiology / Q000473:pathology*; D011897:Random Allocation; D012016:Reference Values; D012307:Risk Factors; D016896:Treatment Outcome; D055357:Uterine Artery Embolization / Q000009:adverse effects / Q000379:methods*; D014626:Vaginal Smears
chemical_list: D000180:Acrylic Resins; C496735:trisacryl gelatin microspheres; D005780:Gelatin