title: Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis.
abstract: Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.
authors.affiliation: Department of Integrated Sciences in Physics and Biology, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Tokyo 156-8550, Japan. nagao.ryo@nihon-u.ac.jp
authors.forename: Ryo
authors.initials: R
authors.lastname: Nagao
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tomo
authors.forename: Rei
authors.initials: R
authors.lastname: Narikawa
authors.forename: Isao
authors.initials: I
authors.lastname: Enami
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
mesh_terms: D000222:Adaptation, Physiological / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D003624:Darkness; D017377:Diatoms / Q000166:cytology / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects*; D008027:Light; D045332:Photosystem II Protein Complex / Q000096:biosynthesis* / Q000737:chemistry*; D055503:Protein Multimerization / Q000528:radiation effects*; D020836:Protein Structure, Quaternary
chemical_list: D045332:Photosystem II Protein Complex