title: Constructing inexpensive, flexible, and versatile microdialysis probes in an undergraduate microdialysis research lab.
abstract: Several challenges await new assistant professors setting up a neuroscience lab, and obtaining sufficient research help is typically a top priority. A secondary, but no less daunting, challenge is juggling accuracy and reliability with costs and limited start-up funds. These concerns are particularly crucial for those engaging technically sophisticated measurements, such as microdialysis. We have developed straightforward procedures that our undergraduate students have utilized to successfully construct high-quality, low-cost microdialysis probes. Students mastering the various steps involved have also gained valuable insight into their use, troubleshooting, and the implications of data obtained from these constructed probes. These procedures are explained here to foster increased use in neuroscience labs that involve undergraduates, along with pointers about teaching the technique to newcomers. Students who master the techniques can pass them on to new students easily. These procedures train students in the overall research technique of microdialysis more thoroughly than when manufactured probes are used, they save money, and will eventually save the principal investigator time when students develop independence with troubleshooting and repairs.
authors.affiliation: Psychology Department and Neuroscience Program, Central Michigan University, Mount Pleasant, MI 48859.
authors.forename: Sally
authors.initials: S
authors.lastname: Steffes
authors.forename: Michael
authors.initials: M
authors.lastname: Sandstrom
keywords: freely-moving; in-vivo; microdialysis; probe design; undergraduate pedagogy