title: Purification of a novel nitric oxide inhibitory peptide derived from enzymatic hydrolysates of Mytilus coruscus.
abstract: Shellfish contain significant levels of high quality protein and are therefore a potential source for biofunctional high-value peptides. To purify a novel anti-inflammatory peptide from Mytilus coruscus (M. coruscus), we applied enzymatic hydrolysis and tangential flow filtration (TFF) and investigated its nitric oxide inhibitory property. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis. Flavouzyme hydrolysates, which showed clearly superior nitric oxide inhibitory activity on lipopolysaccharide (LPS)-stimulated RAW264.7, were further purified using a TFF system and consecutive chromatographic methods. Finally, a novel anti-inflammatory peptide composed of 10 amino acid residues was obtained, and the sequence was identified as Gly-Val-Ser-Leu-Leu-Gln-Gln-Phe-Phe-Leu at N-terminal position. The peptide from M. coruscus effectively inhibited nitric oxide production on macrophage cells. This is the first report of an anti-inflammatory peptide derived from the hydrolysates of M. coruscus.
authors.affiliation: Division of Food Bioscience, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Seo Hee
authors.initials: SH
authors.lastname: Kang
authors.forename: Dong-Kug
authors.initials: DK
authors.lastname: Choi
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Jung Suck
authors.initials: JS
authors.lastname: Lee
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005374:Filtration; D006868:Hydrolysis; D008264:Macrophages / Q000187:drug effects; D049878:Mytilus / Q000737:chemistry / Q000378:metabolism*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D056910:Republic of Korea
chemical_list: D000893:Anti-Inflammatory Agents; D010455:Peptides; D009569:Nitric Oxide