title: A quarter century of reaping what we SOE.
abstract: Gene Splicing by Overlap Extension (SOE), described in a BioTechniques Research Report 23 years ago, is still widely used today in spite of changes in technology and scientific fashion. The history of SOE offers practical lessons for those who seek the best techniques, and for those who strive to develop them.
authors.affiliation: Department of Biochemistry, Emory University School of Medicine.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000266:history / Q000379:methods; D016133:Polymerase Chain Reaction / Q000266:history / Q000379:methods*
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant