title: [Study on prohibition of high mobility group chromosomal protein N2 against human oral squamous cell carcinoma in vitro].
abstract: OBJECTIVE
  Take human oral squamous cell carcinoma Tca8113 as experimental model, and study the anti oral squamous cell carcinoma activity of high mobility group chromosomal protein N2 (HMGN2) molecule.
  
  
  METHODS
  Train a large number of recombinant human HMGN2 expression vector Escherichia coli BL21. HMGN2 was expressed under isopropyl-1-thio-beta-galactopyranoside (IPTG) induction and purified by B-PER GST Fusion Protein Purification Kit. A variety of concentrations HMGN2 were added to cell culture medium, cells were tested by MTT, Hoechst 33342 fluorescence staining, flow cytometry assay and Western-blot.
  
  
  RESULTS
  MTT results proved that HMGN2 could significantly inhibit human oral squamous cell carcinoma Tca8113 growth. Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis.
  
  
  CONCLUSIONS
  HMGN2 can promote apoptosis of oral squamous cell carcinoma cells.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Dong
authors.forename: Xiqian
authors.initials: X
authors.lastname: Liu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Libing
authors.initials: L
authors.lastname: Lu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D049109:Cell Proliferation; D006609:High Mobility Group Proteins; D006801:Humans; D066298:In Vitro Techniques; D009062:Mouth Neoplasms; D011994:Recombinant Proteins
chemical_list: D006609:High Mobility Group Proteins; D011994:Recombinant Proteins