title: Caged mono- and divalent ligands for light-assisted disruption of PDZ domain-mediated interactions.
abstract: We report a general method for light-assisted control of interactions of PDZ domain binding motifs with their cognate domains by the incorporation of a photolabile caging group onto the essential C-terminal carboxylate binding determinant of the motif. The strategy was implemented and validated for both simple monovalent and biomimetic divalent ligands, which have recently been established as powerful tools for acute perturbation of native PDZ domain-dependent interactions in live cells.
authors.affiliation: Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Sainlos
authors.forename: Wendy S
authors.initials: WS
authors.lastname: Iskenderian-Epps
authors.forename: Nelson B
authors.initials: NB
authors.lastname: Olivier
authors.forename: Daniel
authors.initials: D
authors.lastname: Choquet
authors.forename: Barbara
authors.initials: B
authors.lastname: Imperiali
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D008024:Ligands; D008027:Light; D008958:Models, Molecular; D008969:Molecular Sequence Data; D054731:PDZ Domains; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D010782:Photolysis; D011485:Protein Binding; D025941:Protein Interaction Mapping; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D010455:Peptides