title: Association between HLA-DQB1*03:01 and Bullous pemphigoid in Iranian patients.
abstract: BACKGROUND
  A common Human Leukocyte Antigen (HLA) class II allele, DQβ1*03:01, seems to be associated with Bullous pemphigoid (BP) in Caucasians whereas previous studies in other ethnic groups showed other HLA class II alleles as genetic predisposing factors for BP.
  
  
  OBJECTIVE
  To investigate the association of HLA class II alleles and haplotypes with BP in Iranian population.
  
  
  METHODS
  Fifty patients with Bullous pemphigoid and 180 geographically matched, healthy individuals as control group enrolled into this study. HLA typing of class II (DR and DQ alleles) was carried out using polymerase chain reaction based on sequence-specific primers method.
  
  
  RESULTS
  Class II DQA1 and DQB1 typing showed a significantly higher frequency of HLA-DQA1*05:01 (45% vs. 33%, p=0.03), HLA-DQB1*03:01 (36% vs. 23.6%, p=0.02) and HLA-DQB1*04:01 (4% vs. 1.6%, p=0.04) in the BP patients compared with controls. For DRB1 allele frequencies, there were no significant disease associations. The frequency of DRB1*08:01/DQA1*05:01/DQB1*03:01 (3% vs. 0%, p=0.02) haplotype showed an increase among patients compared with controls.
  
  
  CONCLUSIONS
  Our data suggest that Iranian patients with BP present the same genetic predisposition linked to HLA-DQB1*03:01 previously reported in Caucasians.
authors.affiliation: Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran Iran, amirzara@tums.ac.ir.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Esmaili
authors.forename: Hossein
authors.initials: H
authors.lastname: Mortazavi
authors.forename: Cheyda
authors.initials: C
authors.lastname: Chams-Davatchi
authors.forename: Maryam
authors.initials: M
authors.lastname: Daneshpazhooh
authors.forename: Maede Rayati
authors.initials: MR
authors.lastname: Damavandi
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Aryanian
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Amirzargar
mesh_terms: D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006239:Haplotypes; D006650:Histocompatibility Testing; D006801:Humans; D007492:Iran; D008297:Male; D009154:Mutation; D010391:Pemphigoid, Bullous / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen