title: Acetylcholinesterase immobilization on polyacrylamide/functionalized multi-walled carbon nanotube nanocomposite nanofibrous membrane.
abstract: In this work, polyacrylamide/multi-walled carbon nanotubes (MWCNT) solution is electrospun to nanocomposite nanofibrous membranes for acetylcholinesterase enzyme immobilization. A new method for enzyme immobilization is proposed, and the results of analysis show successful covalent bonding of enzymes on electrospun membrane surface besides their non-covalent entrapment. Fourier transform infrared spectroscopy, mechanical and thermal investigations of nanofibrous membrane approve successful cross-linking and enzyme immobilization. The enzyme relative activity and kinetic on both pure and nanocomposite membranes is investigated, and the results show proper performance of designed membrane to even improve the enzyme activity followed by immobilization compared to free enzyme. Scanning electron microscopy images show nanofibrous web of 3D structure with a low shrinkage and hydrogel structure followed by enzyme immobilization and cross-linking. Moreover, the important role of functionalized carbon nanotubes on final nanofibrous membrane functionality as a media for enzyme immobilization is investigated. The results show that MWCNT could act effectively for enzyme immobilization improvement via both physical (enhanced fibers' morphology and conductivity) and chemical (enzyme entrapment) methods.
authors.affiliation: Department of Polymer Engineering, Islamic Azad University, South Tehran Branch, 1777613651 Tehran, Iran.
authors.forename: Navid
authors.initials: N
authors.lastname: Amini
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Mazinani
authors.forename: Seyed-Omid
authors.initials: SO
authors.lastname: Ranaei-Siadat
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Kalaee
authors.forename: Saeed
authors.initials: S
authors.lastname: Hormozi
authors.forename: Kaveh
authors.initials: K
authors.lastname: Niknam
authors.forename: Nasrin
authors.initials: N
authors.lastname: Firouzian
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000235:genetics; D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics; D004331:Drosophila melanogaster / Q000737:chemistry / Q000201:enzymology; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000235:genetics; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D010843:Pichia / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D000099166:Polyacrylamides
chemical_list: D000110:Acetylcholinesterase; D000180:Acrylic Resins; D029721:Drosophila Proteins; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D000099166:Polyacrylamides