title: A new helicase assay based on graphene oxide for anti-viral drug development.
abstract: Recently, graphene oxide (GO), one of the carbon nanomaterials, has received much attention due to its unique physical and chemical properties and high potential in many research areas, including applications as a biosensor and drug delivery vehicle. Various GO-based biosensors have been developed, largely based on its surface adsorption properties for detecting biomolecules, such as nucleotides and peptides, and real-time monitoring of enzymatic reactions. In this review, we discuss recent advances in GO-based biosensors focusing on a novel assay platform for helicase activity, which was also employed in high-throughput screening to discover selective helicase inhibitors.
authors.affiliation: Center for Nanomaterials and Chemical Reactions, Institute for Basic Science, Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Hongje
authors.initials: H
authors.lastname: Jang
authors.forename: Soo-Ryoon
authors.initials: SR
authors.lastname: Ryoo
authors.forename: Min Jae
authors.initials: MJ
authors.lastname: Lee
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Han
authors.forename: Dal-Hee
authors.initials: DH
authors.lastname: Min
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004265:DNA Helicases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D010087:Oxides; D006108:Graphite; D004265:DNA Helicases