title: Metabolic engineering of Escherichia coli: a sustainable industrial platform for bio-based chemical production.
abstract: In order to decrease carbon emissions and negative environmental impacts of various pollutants, more bulk and/or fine chemicals are produced by bioprocesses, replacing the traditional energy and fossil based intensive route. The Gram-negative rod-shaped bacterium, Escherichia coli has been studied extensively on a fundamental and applied level and has become a predominant host microorganism for industrial applications. Furthermore, metabolic engineering of E. coli for the enhanced biochemical production has been significantly promoted by the integrated use of recent developments in systems biology, synthetic biology and evolutionary engineering. In this review, we focus on recent efforts devoted to the use of genetically engineered E. coli as a sustainable platform for the production of industrially important biochemicals such as biofuels, organic acids, amino acids, sugar alcohols and biopolymers. In addition, representative secondary metabolites produced by E. coli will be systematically discussed and the successful strategies for strain improvements will be highlighted. Moreover, this review presents guidelines for future developments in the bio-based chemical production using E. coli as an industrial platform.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education & School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address: xzchen@jiangnan.edu.cn.
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Kangming
authors.initials: K
authors.lastname: Tian
authors.forename: Ashwani
authors.initials: A
authors.lastname: Kumar
authors.forename: Suren
authors.initials: S
authors.lastname: Singh
authors.forename: Bernard A
authors.initials: BA
authors.lastname: Prior
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D056804:Biofuels; D001704:Biopolymers / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007218:Industrial Microbiology; D060847:Metabolic Engineering; D009930:Organic Chemicals / Q000378:metabolism
chemical_list: D056804:Biofuels; D001704:Biopolymers; D009930:Organic Chemicals
keywords: Biochemical product; Bioprocess; Escherichia coli; Industrial platform; Metabolic engineering