title: [Microbiological procedures for the diagnosis, management, and study of invasive fungal infections].
abstract: Invasive fungal infections (IFIs) are difficult to diagnose and cause a high mortality to an expanding spectrum of patients. Culture of clinical samples has limitations for the diagnosis of IFI and alternative procedures have been developed. Among them, serum determination of galactomannan or beta-1,3-d-glucan, and antimicelium and antimannan antibodies are relevant. The use of molecular procedures and mass spectrometry (MALDI-TOF) are encouraging tools for the optimization of the diagnosis and management of patients with IFI. The proposal of species-specific breakpoint to classify the isolates as resistant or susceptible to antifungal agents and the necessity of monitor the azole serum levels deserve greater attention.
authors.affiliation: Servicio de Microbiología, Hospital Universitario y Politécnico La Fe, Valencia, España.
authors.forename: Emilia
authors.initials: E
authors.lastname: Canton
authors.affiliation: Servicio de Microbiología y Parasitología, Hospital Universitario La Paz, Madrid, España.
authors.forename: Julio
authors.initials: J
authors.lastname: García-Rodríguez
authors.affiliation: Unidad de Gestión Clínica de Enfermedades Infecciosas y Microbiología, Hospital de Valme, Sevilla, España.
authors.forename: Estrella
authors.initials: E
authors.lastname: Martín-Mazuelos
authors.affiliation: Servicio de Microbiología, Hospital Universitario y Politécnico La Fe, Valencia, España.
authors.forename: Javier
authors.initials: J
authors.lastname: Pemán
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas-VIH, Hospital General Universitario Gregorio Marañón, Madrid, España. Electronic address: jguineaortega@yahoo.es.
authors.forename: Jesús
authors.initials: J
authors.lastname: Guinea
mesh_terms: D006801:Humans; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques / Q000379:methods; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology*
keywords: Antifungal agents; Antifungal susceptibility; Antifúngicos; Aspergillus; Azoles; Biomarcadores; Biomarkers; Candida; Diagnosis; Diagnóstico; Infección fúngica invasora; Invasive fungal Infection; MALDI-TOF; Sensibilidad antifúngica