title: Comparison of 3 automated immunoassays for detection of anti-hepatitis A virus immunoglobulin M in a tertiary care hospital.
abstract: Three automated immunoassay kits for anti-Hepatitis A Virus (HAV) IgM-Architect, (Abbott Laboratories, USA), Elecsys (Roche Diagnostics, Germany), and ADVIA Centaur (Siemens Healthcare Diagnostics Inc., USA)-were compared. We included 178 consecutive samples, for which an anti-HAV IgM test was requested at Seoul National University Hospital from September 2009 to January 2010. Reviewing of medical records, reverse transcription (RT)-PCR for HAV RNA, or total anti-HAV assay were performed on 16 (9.0%) samples with discrepant results. The percent agreements (kappas) of the Architect and ADVIA Centaur, Architect and Elecsys, and ADVIA Centaur and Elecsys kits were 96.6% (0.91), 96.6% (0.92), and 97.8% (0.94), respectively. Eight out of 16 discrepant samples showed gray-zone values in Architect but were nonreactive in the others. Slightly earlier seroconversion was suspected in Elecsys. The 3 assays showed comparable performances with excellent agreements in a tertiary care hospital setting.
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Park
authors.forename: Yu-joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: Moon-Woo
authors.initials: MW
authors.lastname: Seong
authors.forename: Do-Hoon
authors.initials: DH
authors.lastname: Lee
authors.forename: Myoung Hee
authors.initials: MH
authors.lastname: Park
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
mesh_terms: D030041:Hepatitis A virus / Q000235:genetics / Q000276:immunology*; D006509:Hepatitis B / Q000175:diagnosis*; D006785:Hospitals, University; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D063128:Tertiary Health Care
chemical_list: D007075:Immunoglobulin M; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic
keywords: Agreement; Anti-hepatitis A virus immunoglobulin M; Immunoassay