title: Second-coordination-sphere effects increase the catalytic efficiency of an extended model for Fe(III)M(II) purple acid phosphatases.
abstract: Herein we describe the synthesis of a new heterodinuclear Fe(III)Cu(II) model complex for the active site of purple acid phosphatases and its binding to a polyamine chain, a model for the amino acid residues around the active site. The properties of these systems and their catalytic activity in the hydrolysis of bis(2,4-dinitrophenyl)phosphate are compared, and conclusions regarding the effects of the second coordination sphere are drawn. The positive effect of the polymeric chain on DNA hydrolysis is also described and discussed.
authors.affiliation: Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina 88040-900, Brazil.
authors.forename: Bernardo
authors.initials: B
authors.lastname: de Souza
authors.forename: Gabriel L
authors.initials: GL
authors.lastname: Kreft
authors.forename: Tiago
authors.initials: T
authors.lastname: Bortolotto
authors.forename: Hernán
authors.initials: H
authors.lastname: Terenzi
authors.forename: Adailton J
authors.initials: AJ
authors.lastname: Bortoluzzi
authors.forename: Eduardo E
authors.initials: EE
authors.lastname: Castellano
authors.forename: Rosely A
authors.initials: RA
authors.lastname: Peralta
authors.forename: Josiel B
authors.initials: JB
authors.lastname: Domingos
authors.forename: Ademir
authors.initials: A
authors.lastname: Neves
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry; D055162:Biocatalysis; D020134:Catalytic Domain; D002412:Cations; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D006868:Hydrolysis; D007501:Iron / Q000737:chemistry*; D007700:Kinetics; D008958:Models, Molecular; D018716:Molecular Mimicry; D010940:Plant Proteins / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D002412:Cations; D056831:Coordination Complexes; D006023:Glycoproteins; D010940:Plant Proteins; D011073:Polyamines; D003300:Copper; D004247:DNA; D007501:Iron; C091137:purple acid phosphatase; D000135:Acid Phosphatase