title: Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically driven continuous fluid samplers.
abstract: Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for >1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS's potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions.
authors.affiliation: Harvard University, Department of Organismic and Evolutionary Biology, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA.
authors.forename: Julie
authors.initials: J
authors.lastname: Robidart
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Callister
authors.forename: Pengfei
authors.initials: P
authors.lastname: Song
authors.forename: Carrie D
authors.initials: CD
authors.lastname: Nicora
authors.forename: Charles G
authors.initials: CG
authors.lastname: Wheat
authors.forename: Peter R
authors.initials: PR
authors.lastname: Girguis
mesh_terms: D002853:Chromatography, Liquid; D060086:Hydrothermal Vents; D009995:Osmosis; D040901:Proteomics; D053719:Tandem Mass Spectrometry