title: Selection and identification of a DNA aptamer that mimics saxitoxin in antibody binding.
abstract: In this article, high-affinity single-stranded DNA (ssDNA) aptamer-targeting F(ab')₂ fragments of saxitoxin (STX) antibodies were selected from a random ssDNA library by the SELEX strategy. After 16 rounds of repeated selection, the enriched ssDNA library was sequenced, and all of the sequences were carefully identified by indirect enzyme-linked assay and indirect competitive enzyme-linked assay (icELISA). The candidate aptamers in the above identification were selected for further characterization by icELISA and the equilibrium filtration method. We successfully obtained an aptamer that mimics STX in antibody binding, and a substitute for STX in aptamer form has been developed. Further work is in progress aimed at using this aptamer substitute to replace the STX standard in an antibody-based, nontoxic detection method for field determination of STX in seafood products.
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, Changchun, Jilin 130062, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Hu
authors.forename: Zengshan
authors.initials: Z
authors.lastname: Liu
authors.forename: Ruiyun
authors.initials: R
authors.lastname: Tian
authors.forename: Honglin
authors.initials: H
authors.lastname: Ren
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Lin
authors.forename: Sheng
authors.initials: S
authors.lastname: Gong
authors.forename: Xianmei
authors.initials: X
authors.lastname: Meng
authors.forename: Guangming
authors.initials: G
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shiying
authors.initials: S
authors.lastname: Lu
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D001666:Binding Sites, Antibody; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods; D015723:Gene Library; D012015:Reference Standards; D012530:Saxitoxin / Q000032:analysis / Q000378:metabolism*; D017747:Seafood / Q000032:analysis; D012758:Shellfish / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D012530:Saxitoxin