title: Comparative analysis of microbial profiles in cow rumen fed with different dietary fiber by tagged 16S rRNA gene pyrosequencing.
abstract: The ruminal microbiome of cattle plays an important role not only in animal health and productivity but also in food safety and environment. Microbial profiles of rumen fluid obtained from dairy cows fed on three different fiber/starch diet compositions were characterized. Tagged 16S rRNA gene pyrosequencing and statistical analysis revealed that the dominant ruminal bacteria shared by all three sample groups belonged to phyla Bacteroidetes, Firmicutes, and Proteobacteria. However, the relative abundance of these bacterial groups was markedly affected by diet composition. In animals fed with a high fiber diet, the fibrolytic and cellulolytic bacteria Lachnospiraceae, Ruminococcaceae, and Fibrobacteraceae were found in highest abundance compared with animals fed other diets with lower fiber content. The polysaccharide-degrading Prevotellaceae and Flavobacteriaceae bacteria were most abundant in the rumen of cows fed on diet with the highest starch content. These data highlight the ruminal microbiome's ability to adapt to feed composition and also provide a basis for the development of feed formulation systems designed to improve livestock productivity.
authors.affiliation: Enzyme Technology Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Paholyothin Road, Khlong Luang, Pathumthani, 12120, Thailand. honglada.tho@biotec.or.th
authors.forename: Honglada
authors.initials: H
authors.lastname: Thoetkiattikul
authors.forename: Wuttichai
authors.initials: W
authors.lastname: Mhuantong
authors.forename: Thanaporn
authors.initials: T
authors.lastname: Laothanachareon
authors.forename: Sithichoke
authors.initials: S
authors.lastname: Tangphatsornruang
authors.forename: Virote
authors.initials: V
authors.lastname: Pattarajinda
authors.forename: Lily
authors.initials: L
authors.lastname: Eurwilaichitr
authors.forename: Verawat
authors.initials: V
authors.lastname: Champreda
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics*; D004043:Dietary Fiber / Q000032:analysis / Q000378:metabolism*; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012417:Rumen / Q000378:metabolism / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004043:Dietary Fiber; D012336:RNA, Ribosomal, 16S