title: Canine osteosarcoma cell lines from patients with differing serum alkaline phosphatase concentrations display no behavioural differences in vitro.
abstract: Osteosarcoma is an aggressive malignancy and represents the most frequent primary bone malignancy of dogs and humans. Prognostic factors reported for osteosarcoma include tumour size, presence of metastatic disease and serum alkaline phosphatase (ALP) concentration at the time of diagnosis. To date, there have been no studies to determine whether the behaviour of osteosarcoma cells differ based on serum ALP concentration. Here, we report on the generation of six canine osteosarcoma cell lines from osteosarcoma-bearing dogs with differences in serum ALP concentration. To determine whether in vitro behaviour differs between primary osteosarcoma cell lines generated from patients with normal or increased serum ALP, assays were performed to evaluate proliferation, migration, invasion and chemosensitivity. There were no significant differences in cell proliferation, migration, invasion or chemosensitivity between cell lines associated with normal or increased serum ALP concentration.
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Holmes
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: V
authors.initials: V
authors.lastname: Thompson
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Piskun
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Kohnken
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Huelsmeyer
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Fan
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.|Institute for Clinical & Translational Research, University of Wisconsin-Madison, Madison, WI, USA.|Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Stein
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D000704:Analysis of Variance; D000818:Animals; D001859:Bone Neoplasms / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000097:blood* / Q000503:physiopathology*; D004285:Dogs; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D012516:Osteosarcoma / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D011379:Prognosis
chemical_list: D000469:Alkaline Phosphatase
keywords: comparative oncology; in vitro models; oncology; small animal; tumour biology