title: Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus.
abstract: The Meliaceae family of plants has been shown to contain a vast number of compounds with the potential to be developed for medicinal purposes. We have previously reported the isolation of limonoids from a plant in the Meliaceae family named Chisocheton ceramicus. Ceramicine B was identified as an active compound in inhibiting lipid droplets accumulation (LDA) in the mouse preadipocyte cell line MC3T3-G2/PA6. The presence of ceramicine B was found to inhibit the expression of glucose transporter type 4, lipoprotein lipase, and 11-beta hydroxysteroid dehydrogenase mRNA, and also adipogenic master regulator, peroxisome proliferator-activated receptor-γ, and CCAAT-enhancer-binding protein-α (C/EBPα) mRNA. However, for early adipogenic regulators, such as C/EBPβ and C/EBPδ, and intermediary adipogenic regulators, Krüppel-like factors were unaffected. Western blot analysis showed that ceramicine B was found to inhibit the phosphorylation of Forkhead box O1 (Foxo1), a key process in the insulin signaling pathway. This suggested that the mechanism of anti-LDA activity of ceramicine B was partly via the inhibition of Foxo1 phosphorylation.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo, 142-8501, Japan.
authors.forename: Chin Piow
authors.initials: CP
authors.lastname: Wong
authors.forename: Toshio
authors.initials: T
authors.lastname: Kaneda
authors.forename: A Hamid A
authors.initials: AH
authors.lastname: Hadi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morita
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000302:isolation & purification / Q000494:pharmacology*; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D036701:Limonins / Q000302:isolation & purification / Q000494:pharmacology*; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D029582:Meliaceae / Q000737:chemistry; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D019440:Anti-Obesity Agents; D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; D051275:Glucose Transporter Type 4; D051741:Kruppel-Like Transcription Factors; D036701:Limonins; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; C495693:Slc2a4 protein, mouse; C000588427:ceramicine B; D042842:11-beta-Hydroxysteroid Dehydrogenases; D008071:Lipoprotein Lipase