title: Bioimaging of peroxynitrite in MCF-7 cells by a new fluorescent probe rhodamine B phenyl hydrazide.
abstract: Peroxynitrite is a potent oxidizing and nitrating agent which has detrimental effects on cells by altering the structure and function of biomolecules present within. A fluorescent probe rhodamine B phenyl hydrazide (RBPH) has been proposed for peroxynitrite (ONOO(-)) imaging in MCF-7 cells based on its oxidation property, which converts RBPH to pink colored and highly fluorescent rhodamine B. The fluorescence emission intensity of the rhodamine B produced in the above process is linearly related to the concentration of peroxynitrite. The method obeys Beer's law in the concentration range 2-20 nM and the detection limit has been found to be 1.4 nM. The possible reaction mechanism of peroxynitrite with RBPH to produce rhodamine B has been discussed with spectroscopic evidence. The Probe is selective to the peroxynitrite in the pH range 6-8 which is near physiological pH. Fluorescence microscopic studies suggest that the probe is cell permeable and hence peroxynitrite was imaged in MCF-7 cells.
authors.affiliation: Department of Microbiology & Biotechnology, Bangalore University, Jnanabharathi Campus, Bangalore, 560 056, India.
authors.forename: Gopi
authors.initials: G
authors.lastname: Ambikapathi
authors.forename: Suresh Kumar
authors.initials: SK
authors.lastname: Kempahanumakkagari
authors.forename: Babu
authors.initials: B
authors.lastname: Ramappa Lamani
authors.forename: Devaraju
authors.initials: D
authors.lastname: Kuramkote Shivanna
authors.forename: Harish
authors.initials: H
authors.lastname: Bodagur Maregowda
authors.forename: Anushree
authors.initials: A
authors.lastname: Gupta
authors.forename: Pandurangappa
authors.initials: P
authors.lastname: Malingappa
mesh_terms: D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D061986:MCF-7 Cells; D057054:Molecular Imaging; D030421:Peroxynitrous Acid / Q000737:chemistry* / Q000378:metabolism*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D006834:Hydrazines; D012235:Rhodamines; C543681:rhodamine B hydrazide; D030421:Peroxynitrous Acid