title: Potent lipolytic activity of lactoferrin in mature adipocytes.
abstract: Lactoferrin (LF) is a multifunctional glycoprotein found in mammalian milk. We have shown in a previous clinical study that enteric-coated bovine LF tablets decreased visceral fat accumulation. To address the underlying mechanism, we conducted in vitro studies and revealed the anti-adipogenic action of LF in pre-adipocytes. The aim of this study was to assess whether LF could increase the lipolytic activity in mature adipocytes. Pre-adipocytes were prepared from rat mesenteric fat and differentiated into mature adipocytes for assays of lipolysis. The addition of LF significantly increased the glycerol concentration in the medium in a dose-dependent manner, whereas pepsin-degraded LF did not. A DNA microarray analysis demonstrated that LF decreased the expression of perilipin and affected the cAMP pathway. These findings are supported by the results of quantitative RT-PCR of perilipin and assays of cAMP. These data collectively indicate that visceral fat reduction by LF may result from the promotion of lipolysis and the additional anti-adipogenic activity of LF.
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan. tomoono@lion.co.jp
authors.forename: Tomoji
authors.initials: T
authors.lastname: Ono
authors.forename: Chikako
authors.initials: C
authors.lastname: Fujisaki
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Ishihara
authors.forename: Keiko
authors.initials: K
authors.lastname: Ikoma
authors.forename: Satoru
authors.initials: S
authors.lastname: Morishita
authors.forename: Michiaki
authors.initials: M
authors.lastname: Murakoshi
authors.forename: Keikichi
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Hisanori
authors.initials: H
authors.lastname: Kato
authors.forename: Kazuo
authors.initials: K
authors.lastname: Miyashita
authors.forename: Toshihide
authors.initials: T
authors.lastname: Yoshida
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D007781:Lactoferrin / Q000378:metabolism / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects* / Q000235:genetics; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D059748:Proteolysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D059467:Transcriptome / Q000187:drug effects
chemical_list: D007781:Lactoferrin