title: The terpolymer produced by Azotobacter chroococcum 7B: effect of surface properties on cell attachment.
abstract: The copolymerization of poly(3-hydroxybutyrate) (PHB) is a promising trend in bioengineering to improve biomedical properties, e.g. biocompatibility, of this biodegradable polymer. We used strain Azotobacter chroococcum 7B, an effective producer of PHB, for biosynthesis of not only homopolymer and its main copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV), but also novel terpolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-poly(ethylene glycol) (PHB-HV-PEG), using sucrose as the primary carbon source and valeric acid and poly(ethylene glycol) 300 (PEG 300) as additional carbon sources. The chemical structure of PHB-HV-PEG was confirmed by (1)H nuclear-magnetic resonance analysis. The physico-chemical properties (molecular weight, crystallinity, hydrophilicity, surface energy) of produced biopolymer, the protein adsorption to the terpolymer, and cell growth on biopolymer films were studied. Despite of low EG-monomers content in bacterial-origin PHB-HV-PEG polymer, the terpolymer demonstrated significant improvement in biocompatibility in vitro in contrast to PHB and PHB-HV polymers, which may be coupled with increased protein adsorption, hydrophilicity and surface roughness of PEG-containing copolymer.
authors.affiliation: Faculty of Biology, Moscow State University, Moscow, Russia. ant_bonar@mail.ru
authors.forename: Anton
authors.initials: A
authors.lastname: Bonartsev
authors.forename: Sergey
authors.initials: S
authors.lastname: Yakovlev
authors.forename: Arasha
authors.initials: A
authors.lastname: Boskhomdzhiev
authors.forename: Irina
authors.initials: I
authors.lastname: Zharkova
authors.forename: Dmitrii
authors.initials: D
authors.lastname: Bagrov
authors.forename: Vera
authors.initials: V
authors.lastname: Myshkina
authors.forename: Tatiana
authors.initials: T
authors.lastname: Mahina
authors.forename: Elena
authors.initials: E
authors.lastname: Kharitonova
authors.forename: Olga
authors.initials: O
authors.lastname: Samsonova
authors.forename: Anton
authors.initials: A
authors.lastname: Zernov
authors.forename: Vsevolod
authors.initials: V
authors.lastname: Zhuikov
authors.forename: Yurii
authors.initials: Y
authors.lastname: Efremov
authors.forename: Vera
authors.initials: V
authors.lastname: Voinova
authors.forename: Garina
authors.initials: G
authors.lastname: Bonartseva
authors.forename: Konstantin
authors.initials: K
authors.lastname: Shaitan
mesh_terms: D001395:Azotobacter / Q000378:metabolism*; D001672:Biocompatible Materials; D001704:Biopolymers / Q000096:biosynthesis / Q000502:physiology*; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion; D049109:Cell Proliferation; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D008970:Molecular Weight
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers