title: High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations.
abstract: OBJECTIVE
  This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation.
  
  
  METHODS
  We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as "ICSI-like", and (iii) with MSOME.
  
  
  RESULTS
  The balanced translocation rates did not differ significantly (p=0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p=0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %).
  
  
  CONCLUSIONS
  For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.
authors.affiliation: Department of Reproductive Biology, Cytogenetics, Gynaecology and Obstetrics, CHIPS, Centre Hospitalier Poissy Saint-Germain, 10 rue Champ-Gaillard, 78303, Poissy, France.
authors.forename: Mohamed Hassen
authors.initials: MH
authors.lastname: Chelli
authors.forename: Fatma
authors.initials: F
authors.lastname: Ferfouri
authors.forename: Florence
authors.initials: F
authors.lastname: Boitrelle
authors.forename: Martine
authors.initials: M
authors.lastname: Albert
authors.forename: Denise
authors.initials: D
authors.lastname: Molina-Gomes
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Selva
authors.forename: François
authors.initials: F
authors.lastname: Vialard
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D008875:Middle Aged; D055101:Semen Analysis; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology* / Q000648:ultrastructure; D014178:Translocation, Genetic