title: Deriving dopaminergic neurons for clinical use. A practical approach.
abstract: New small molecules that regulate the step-wise differentiation of human pluripotent stem cells into dopaminergic neurons have been identified. The steroid, guggulsterone, was found to be the most effective inducer of neural stem cells into dopaminergic neurons. These neurons are extensively characterized and shown to be functional. We believe this new approach offers a practical route to creating neurons of sufficient quality to be used to treat Parkinson's disease patients.
authors.affiliation: International Stem Cell Corporation, 5950 Priestly Drive, Carlsbad, CA 92008, USA.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Gonzalez
authors.forename: Ibon
authors.initials: I
authors.lastname: Garitaonandia
authors.forename: Tatiana
authors.initials: T
authors.lastname: Abramihina
authors.forename: Gerald K
authors.initials: GK
authors.lastname: Wambua
authors.forename: Alina
authors.initials: A
authors.lastname: Ostrowska
authors.forename: Mathew
authors.initials: M
authors.lastname: Brock
authors.forename: Alexander
authors.initials: A
authors.lastname: Noskov
authors.forename: Francesca S
authors.initials: FS
authors.lastname: Boscolo
authors.forename: John S
authors.initials: JS
authors.lastname: Craw
authors.forename: Louise C
authors.initials: LC
authors.lastname: Laurent
authors.forename: Evan Y
authors.initials: EY
authors.lastname: Snyder
authors.forename: Ruslan A
authors.initials: RA
authors.lastname: Semechkin
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D017690:Cell Transplantation / Q000379:methods; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D020869:Gene Expression Profiling; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D010300:Parkinson Disease / Q000601:surgery; D018408:Patch-Clamp Techniques; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D011282:Pregnenediones / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011282:Pregnenediones; C023617:pregna-4,17-diene-3,16-dione; D004298:Dopamine