title: Colorimetric test-systems for creatinine detection based on composite molecularly imprinted polymer membranes.
abstract: An easy-to-use colorimetric test-system for the efficient detection of creatinine in aqueous samples was developed. The test-system is based on composite molecularly imprinted polymer (MIP) membranes with artificial receptor sites capable of creatinine recognition. A thin MIP layer was created on the surface of microfiltration polyvinylidene fluoride (PVDF) membranes using method of photo-initiated grafting polymerization. The MIP layer was obtained by co-polymerization of a functional monomer (e.g. 2-acrylamido-2-methyl-1-propanesulfonic acid, itaconic acid or methacrylic acid) with N, N'-methylenebisacrylamide as a cross-linker. The choice of the functional monomer was based on the results of computational modeling. The creatinine-selective composite MIP membranes were used for measuring creatinine in aqueous samples. Creatinine molecules were selectively adsorbed by the MIP membranes and quantified using color reaction with picrates. The intensity of MIP membranes staining was proportional to creatinine concentration in an analyzed sample. The colorimetric test-system based on the composite MIP membranes was characterized with 0.25 mM detection limit and 0.25-2.5mM linear dynamic range. Storage stability of the MIP membranes was estimated as at least 1 year at room temperature. As compared to the traditional methods of creatinine detection the developed test-system is characterized by simplicity of operation, small size and low cost.
authors.affiliation: Institute of Molecular Biology and Genetics, Kiev, Ukraine. t_sergeyeva@yahoo.co.uk
authors.forename: T A
authors.initials: TA
authors.lastname: Sergeyeva
authors.forename: L A
authors.initials: LA
authors.lastname: Gorbach
authors.forename: E V
authors.initials: EV
authors.lastname: Piletska
authors.forename: S A
authors.initials: SA
authors.lastname: Piletsky
authors.forename: O O
authors.initials: OO
authors.lastname: Brovko
authors.forename: L A
authors.initials: LA
authors.lastname: Honcharova
authors.forename: O D
authors.initials: OD
authors.lastname: Lutsyk
authors.forename: L M
authors.initials: LM
authors.lastname: Sergeeva
authors.forename: O A
authors.initials: OA
authors.lastname: Zinchenko
authors.forename: A V
authors.initials: AV
authors.lastname: El'skaya
mesh_terms: D000328:Adult; D003124:Colorimetry / Q000191:economics; D003198:Computer Simulation; D003404:Creatinine / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D054802:Molecular Imprinting; D015394:Molecular Structure; D011145:Polyvinyls / Q000737:chemistry; D014867:Water / Q000737:chemistry; D005465:Fluorocarbon Polymers
chemical_list: D003404:Creatinine; D008567:Membranes, Artificial; D011145:Polyvinyls; D014867:Water; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers