title: Preparation and characterization of sodium alginate modified with collagen peptides.
abstract: Collagen peptides grafted sodium alginate (SA-COP) was prepared by reacting alginate with collagen peptide via amide linkage in presence of 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy sulfosuccinimide (NHS). The reaction conditions affected the degree of substitution (DS) were studied including the mass ratio of collagen peptide to sodium alginate, reaction temperature and reaction time. The hydrogen peroxide scavenging activity could be controlled by adjusting the DS, concentration and molecular weight. MTT assay was used to investigate the cell viability of SA-COP. The results indicated that the SA-COP exhibited better cell viability, and with the DS and concentration increasing of SA-COP, cell viability increased. The improved functionalities of the derivative might be explained by peptides characteristics.
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China. lihongfan2000@hotmail.com
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.forename: Mi
authors.initials: M
authors.lastname: Cao
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Tan
authors.initials: T
authors.lastname: Wang
authors.forename: Huan
authors.initials: H
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Nie
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry*; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects; D016166:Free Radical Scavengers / Q000138:chemical synthesis / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D008422:Materials Testing / Q000379:methods*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013388:Succinimides / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D016166:Free Radical Scavengers; D006603:Hexuronic Acids; D010455:Peptides; D013388:Succinimides; D020723:Glucuronic Acid; D003094:Collagen; D006861:Hydrogen Peroxide; D005022:Ethyldimethylaminopropyl Carbodiimide