title: Screening of early antigen genes of adult-stage Trichinella spiralis using pig serum from different stages of early infection.
abstract: The goal of this work was to identify novel, early antigens present in Trichinella spiralis. To this end, a cDNA library generated from 3-day old adult worms (Ad3) was immunologically screened using serum from a pig infected with 20,000 muscle larvae. The serum was obtained from multiple, time course bleeds coinciding with early worm development. Seventeen positive clones were isolated using serum obtained at 20 days post infection (dpi). All clones corresponded to one gene that exhibited high sequence identity with the T. spiralis ATP-dependent RNA helicase DDX19B which is involved in parasite growth and development. In addition, nine additional positive clones representing 5 unique genes were identified when the library was screened with 30 dpi serum; four of these five genes displayed high similarity with members of a putative T. spiralis serine protease family known to be involved in host invasion and host-parasite interactions. The remaining gene aligned with the T. spiralis hypothetical ORF 11.30. The identification of these antigens provides potential candidates for the early diagnosis of trichinellosis and for the development of a vaccine against this parasite.
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Jilin University, Zoonosis Center of National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, China.
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: X P
authors.initials: XP
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Bai
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: B
authors.initials: B
authors.lastname: Rosenthal
authors.forename: R
authors.initials: R
authors.lastname: Blaga
authors.forename: S
authors.initials: S
authors.lastname: Lacour
authors.forename: I
authors.initials: I
authors.lastname: Vallee
authors.forename: P
authors.initials: P
authors.lastname: Boireau
authors.forename: C
authors.initials: C
authors.lastname: Gherman
authors.forename: M
authors.initials: M
authors.lastname: Oltean
authors.forename: X N
authors.initials: XN
authors.lastname: Zhou
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: M Y
authors.initials: MY
authors.lastname: Liu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D042241:Early Diagnosis; D005260:Female; D015723:Gene Library; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007814:Larva; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009132:Muscles / Q000469:parasitology; D020365:RNA Helicases / Q000235:genetics / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D013552:Swine; D017160:Trichinella spiralis / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D020365:RNA Helicases