title: Evaluation on a Streptococcus suis vaccine using recombinant sao-l protein manufactured by bioreactors as the antigen in pigs.
abstract: Streptococcus suis (S. suis) can be classified into 33 serotypes based on the structure of capsular polysaccharides. Recent research indicated that a new surface protein designated as Sao (surface antigen one) reacts with 30 serotypes of convalescent-phase sera during S. suis infections, which makes Sao a good potential antigen for developing S. suis vaccines. The objectives of this study were to produce recombinant Sao-L protein (rSao-L) from a strain of S. suis serotype 2 by a prokaryotic expression system in bioreactors and to use rSao-L as the antigen for a S. suis vaccine in mouse and swine models. The antibody titres in mice and pigs immunized with rSao-L were significantly (P < 0.05) increased. After challenge with live S. suis serotype 1 bacteria, the anatomical lesions in pigs immunized with rSao-L were reduced by 60%. These data indicated that immunization with rSao-L can confer cross-serotype protection against S. suis. Moreover, percentages of CD8(+) and CD4(+) /CD8(+) double-positive T cells in immunized pigs were significantly higher than those of the control group (P < 0.01). Using bioreactors to produce rSao-L as the antigen for S. suis vaccines may broaden protective efficacy and reduce production costs.
authors.affiliation: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, Pintung, Taiwan; Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pintung, Taiwan.
authors.forename: K-J
authors.initials: KJ
authors.lastname: Hsueh
authors.forename: J-W
authors.initials: JW
authors.lastname: Lee
authors.forename: S-M
authors.initials: SM
authors.lastname: Hou
authors.forename: H-S
authors.initials: HS
authors.lastname: Chen
authors.forename: T-C
authors.initials: TC
authors.lastname: Chang
authors.forename: C-Y
authors.initials: CY
authors.lastname: Chu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000276:immunology*; D019149:Bioreactors; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D011994:Recombinant Proteins / Q000276:immunology; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000276:immunology*; D017011:Streptococcus suis / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D022541:Streptococcal Vaccines
keywords: Sao; Streptococcus suis; recombinant protein; vaccine