title: Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions.
abstract: Excessive trafficking of leukocytes can lead to serious tissue injury. Here, four regioselectively sulfated chitosans were assessed as inhibitors of HL-60 leukocyte binding to P-selectin, by investigating their effect on leukocyte adhesion to CHO cells expressing human P-selectin under static and flow conditions. The results show that the sulfochitosans exhibit inhibitory activity in this general order: heparin > N-sulfated/6-O-sulfated chitosan ≥ 3-O,6-O-sulfated chitosan > 6-O-sulfated chitosan >> N-sulfated chitosan. This suggests that the sulfation of the double site in chitosan is essential for efficient inhibition of P-selectin-mediated HL-60 leukocyte adhesion and that such sulfochitosans may have potential as therapeutic agents against inflammatory disease.
authors.affiliation: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun, P R China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Huang
authors.forename: Ruifei
authors.initials: R
authors.lastname: Wang
authors.forename: Ximing
authors.initials: X
authors.lastname: Liu
authors.forename: Xianlu
authors.initials: X
authors.lastname: Zeng
authors.forename: Min
authors.initials: M
authors.lastname: Wei
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D018922:HL-60 Cells; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D019007:P-Selectin / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012212:Rheology / Q000187:drug effects*
chemical_list: D019007:P-Selectin; C480355:chitosan sulfate; D048271:Chitosan