title: Structural characterization of an immunoenhancing heteroglycan of a hybrid mushroom (pfls1h) of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer.
abstract: An immunostimulating water-soluble heteroglycan (PS-II) was isolated from an aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h produced by intergeneric protoplast fusion between Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-II was carried out using sugar analysis, methylation experiment, periodate oxidation and 1D/2D NMR studies. Sugar analysis indicated the presence of glucose, mannose and galactose in a molar ratio of 1:1:2. Methylation analysis revealed that PS-II was composed of (1→6)- and (1→2,4,6)-α-D-galactopyranosyl, terminal β-D-mannopyranosyl and terminal β-D-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. On the basis of chemical analysis and NMR studies, the structure of the repeating unit of the heteroglycan was established to consist of a backbone chain of two (1→6)-α-D-galactopyranosyl residues, one of which is branched at O-2 with terminal β-D-Manp and at O-4 with terminal β-D-Glcp. This heteroglycan (PS-II) showed in vitro splenocyte, thymocyte and macrophage activations.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.forename: Praloy K
authors.initials: PK
authors.lastname: Maji
authors.forename: Birendra
authors.initials: B
authors.lastname: Behera
authors.forename: Pijush
authors.initials: P
authors.lastname: Mallick
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002678:Chimera / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020074:Lentinula / Q000737:chemistry* / Q000235:genetics; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008358:Mannose / Q000737:chemistry; D051379:Mice; D020076:Pleurotus / Q000737:chemistry* / Q000235:genetics; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012995:Solubility; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014867:Water
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides; D014867:Water; D005947:Glucose; D008358:Mannose; D005690:Galactose