title: The resolving power of in vitro genotoxicity assays for cigarette smoke particulate matter.
abstract: In vitro genotoxicity assays are often used to compare tobacco smoke particulate matter (PM) from different cigarettes. The quantitative aspect of the comparisons requires appropriate statistical methods and replication levels, to support the interpretation in terms of power and significance. This paper recommends a uniform statistical analysis for the Ames test, mouse lymphoma mammalian cell mutation assay (MLA) and the in vitro micronucleus test (IVMNT); involving a hierarchical decision process with respect to slope, fixed effect and single dose comparisons. With these methods, replication levels of 5 (Ames test TA98), 4 (Ames test TA100), 10 (Ames test TA1537), 6 (MLA) and 4 (IVMNT) resolved a 30% difference in PM genotoxicity.
authors.affiliation: British American Tobacco, Group Research and Development, Regents Park Road, Southampton SO15 8TL, UK. ken_scott@bat.com
authors.forename: K
authors.initials: K
authors.lastname: Scott
authors.forename: J
authors.initials: J
authors.lastname: Saul
authors.forename: I
authors.initials: I
authors.lastname: Crooks
authors.forename: O M
authors.initials: OM
authors.lastname: Camacho
authors.forename: D
authors.initials: D
authors.lastname: Dillon
authors.forename: C
authors.initials: C
authors.lastname: Meredith
mesh_terms: D000818:Animals; D002460:Cell Line; D003627:Data Interpretation, Statistical; D051379:Mice; D009152:Mutagenicity Tests / Q000706:statistics & numerical data*; D052638:Particulate Matter / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012906:Smoke / Q000009:adverse effects*
chemical_list: D052638:Particulate Matter; D012906:Smoke