title: In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss.
abstract: Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC₅₀ (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL⁻¹ and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g⁻¹ of dry extract. Fe²⁺-induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca²⁺-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Δψm only at high concentrations (40-100 mg mL⁻¹), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.
authors.affiliation: Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil. kamdemjeanpaul2005@yahoo.fr
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Kamdem
authors.forename: Sílvio Terra
authors.initials: ST
authors.lastname: Stefanello
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Caroline
authors.initials: C
authors.lastname: Wagner
authors.forename: Ige Joseph
authors.initials: IJ
authors.lastname: Kade
authors.forename: Romaiana Picada
authors.initials: RP
authors.lastname: Pereira
authors.forename: Alessandro De Souza
authors.initials: Ade S
authors.lastname: Preste
authors.forename: Daniel Henrique
authors.initials: DH
authors.lastname: Roos
authors.forename: Emily Pansera
authors.initials: EP
authors.lastname: Waczuk
authors.forename: Andre Storti
authors.initials: AS
authors.lastname: Appel
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.forename: Diogo Onofre
authors.initials: DO
authors.lastname: Souza
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: Rocha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001938:Brazil; D020834:Ethnopharmacology; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D009994:Osmolar Concentration; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D012997:Solvents / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012997:Solvents