title: Role of formyl peptide receptor 2 on the serum amyloid A-induced macrophage foam cell formation.
abstract: Recently we demonstrated that SAA induces macrophage foam cell formation. In this study we show that SAA-induced foam cell formation is inhibited by formyl peptide receptor 2 (FPR2) antagonist WRW(4), as well as by FPR2-targeted siRNA knockdown. SAA-stimulated LOX1 expression was also mediated by FPR2. We also found that SAA-stimulated foam cell formation and LOX1 expression was pertussis toxin-insensitive. In addition, FPR2 is upregulated in peripheral blood mononuclear cells from patients with atherosclerosis. Our findings therefore suggest that SAA stimulates foam cell formation via FPR2 signaling and LOX1 induction, and thus likely contributes to atherogenesis.
authors.affiliation: Department of Biological Science, Sungkyunkwan University, Suwon 440-746, South Korea. hayoung@skku.edu
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Sang Doo
authors.initials: SD
authors.lastname: Kim
authors.forename: Suk-Hwan
authors.initials: SH
authors.lastname: Baek
authors.forename: Joon Hyuk
authors.initials: JH
authors.lastname: Choi
authors.forename: Yoe-Sik
authors.initials: YS
authors.lastname: Bae
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D005487:Foam Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D037342:Pertussis Toxin / Q000494:pharmacology; D044042:Receptors, Formyl Peptide / Q000235:genetics* / Q000502:physiology*; D044067:Receptors, Lipoxin / Q000235:genetics*; D051127:Scavenger Receptors, Class E / Q000235:genetics / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000378:metabolism* / Q000494:pharmacology; D051195:Toll-Like Receptor 2 / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000235:genetics
chemical_list: C105101:FPR2 protein, human; C495138:Olr1 protein, mouse; D044042:Receptors, Formyl Peptide; D044067:Receptors, Lipoxin; D051127:Scavenger Receptors, Class E; D000685:Serum Amyloid A Protein; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C481578:formyl peptide receptor 2, mouse; D037342:Pertussis Toxin