title: [Impact of aging on the secretion of insulin-like growth factor-1 in the corpus cavernosum smooth muscle cells of rats in vitro].
abstract: OBJECTIVE
  To observe the secretion of insulin-like growth factor-1 (IGF-1) in corpus cavernosum smooth muscle cells (CCSMCs) in rats of different ages and explore the possible relationship of IGF-1 with aging-related erectile dysfunction (ED).
  
  
  METHODS
  We primarily cultured CCSMCs of rats aged 4, 12 and 24 months, and identified them by immunohistochemistry. We quantitatively cultured the CCSMCs in 6-well culture plates, determined the levels of IGF-1 secreted from the CCSMCs by enzyme immunoassay (EIA) and analyzed the effect of age on the IGF-1 level.
  
  
  RESULTS
  CCSMCs were successfully cultured in vitro. The level of IGF-1 secreted from the CCSMCs was decreased with the increase of age, with 7.1 ng/10(5) cells in the 4-month-old group, 2.2 ng/10(5) cells in the 12-month group, and 1.9 ng/10(5) cells in the 24-month group, with statistically significant differences among the three groups (P < 0.01).
  
  
  CONCLUSIONS
  The secretion of IGF-1 is reduced with the increase of age, and the decreased expression of IGF-1 might be associated with aging-related ED.
authors.affiliation: Department of Urology, Guangdong Provincial Peoples Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China. lyxd2879@sina.com
authors.forename: Yao-Xiong
authors.initials: YX
authors.lastname: Luo
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Pu
authors.forename: Jiu-Min
authors.initials: JM
authors.lastname: Liu
authors.forename: Xiang-Guang
authors.initials: XG
authors.lastname: Zheng
authors.forename: Huai-Peng
authors.initials: HP
authors.lastname: Wang
mesh_terms: D000375:Aging; D000818:Animals; D002478:Cells, Cultured; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D010413:Penis / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007334:Insulin-Like Growth Factor I