title: A large scale molecular study of Giardia duodenalis in horses from Colombia.
abstract: The prevalence of Giardia duodenalis assemblages in horses is poorly known. The present study examined feces from 195 horses, 1 month-17 years of age, in 4 locations in Colombia. Prevalence of infection was determined by PCR and all positives were sequenced to determine the genotypes. Thirty four (17.4%) horses were found positive. This is the first report of G. duodenalis in horses from Colombia. Prevalence in female and male horses was 18.9% and 15.1%, respectively. Prevalence in horses <1 year of age and horses >1 year of age was 21.1% and 15.1%, respectively. Molecular characterization using the beta giardin (bg), glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), and small subunit ribosomal RNA (ssurRNA) genes identified G. duodenalis Assemblages A and B, the assemblages regarded as zoonotic.
authors.affiliation: Environmental Microbial and Food Safety Laboratory, ARS, US Department of Agriculture, Beltsville, MD, USA. monica.santin-duran@ars.usda.gov
authors.forename: Mónica
authors.initials: M
authors.lastname: Santín
authors.forename: Jesús A
authors.initials: JA
authors.lastname: Cortés Vecino
authors.forename: Ronald
authors.initials: R
authors.lastname: Fayer
mesh_terms: D000375:Aging; D000818:Animals; D003105:Colombia / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005260:Female; D016829:Giardia lamblia / Q000235:genetics*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology*; D006736:Horses; D008297:Male; D015995:Prevalence
keywords: Assemblage; Genotype; Giardia duodenalis; Horse; PCR; Prevalence