title: Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine, and grape juice.
abstract: A simple, reliable, and low-cost method based on molecularly imprinted polymer as a selective sorbent of SPE was proposed for the determination of ochratoxin A (OTA) in beer, red wine, and grape juice by HPLC coupled with fluorescence detection (HPLC-FLD). Samples were diluted with water and cleaned up with an AFFINIMIP® SPE OTA column. After washing and eluting, the analyte was analyzed by HPLC-FLD. Under the optimized conditions, LOD and LOQ for OTA were 0.025 and 0.08 ng/mL, respectively. The recoveries of OTA from beer, red wine, and grape spiked at 0.1, 2, and 5 ng/mL ranged from 91.6 to 101.7%. Furthermore, after a simple regenerated procedure, the molecularly imprinted polymer based SPE column could be reused at least 14 times to achieve more than 80% recoveries of OTA in real samples. The developed method was applied to the detection of 30 beer, red wine, and grape juice samples and only four samples were contaminated by OTA with levels below the legal limits.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P R China.
authors.forename: Jiliang
authors.initials: J
authors.lastname: Cao
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.forename: Shujun
authors.initials: S
authors.lastname: Zhou
authors.forename: Lihui
authors.initials: L
authors.lastname: Yin
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
mesh_terms: D001515:Beer; D001628:Beverages / Q000032:analysis*; D005524:Food Technology / Q000379:methods*; D057230:Limit of Detection; D054802:Molecular Imprinting; D009793:Ochratoxins / Q000032:analysis* / Q000592:standards; D011108:Polymers / Q000032:analysis; D027843:Vitis / Q000737:chemistry; D014920:Wine
chemical_list: D009793:Ochratoxins; D011108:Polymers; C025589:ochratoxin A