title: Effect of human skin explants on the neurite growth of the PC12 cell line.
abstract: The skin is a densely innervated organ. After a traumatic injury, such as an amputation, burn or skin graft, nerve growth and the recovery of sensitivity take a long time and are often incomplete. The roles played by growth factors and the process of neuronal growth are crucial. We developed an in vitro model of human skin explants co-cultured with a rat pheochromocytoma cell line differentiated in neuron in presence of nerve growth factor (NGF). This model allowed the study of the influence of skin explants on nerve cells and nerve fibre growth, probably through mediators produced by the explant, in a simplified manner. The neurite length of differentiated PC12 cells co-cultured with skin explants increased after 6 days. These observations demonstrated the influence of trophic factors produced by skin explants on PC12 cells.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lebonvallet
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Pennec
authors.forename: Christelle
authors.initials: C
authors.lastname: Le Gall
authors.forename: Ulysse
authors.initials: U
authors.lastname: Pereira
authors.forename: Nicholas
authors.initials: N
authors.lastname: Boulais
authors.forename: Jeremy
authors.initials: J
authors.lastname: Cheret
authors.forename: Christine
authors.initials: C
authors.lastname: Jeanmaire
authors.forename: Louis
authors.initials: L
authors.lastname: Danoux
authors.forename: Gilles
authors.initials: G
authors.lastname: Pauly
authors.forename: Laurent
authors.initials: L
authors.lastname: Misery
mesh_terms: D000818:Animals; D001706:Biopsy; D002450:Cell Communication / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D006801:Humans; D023421:Models, Animal; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000473:pathology*; D016716:PC12 Cells; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000473:pathology*
chemical_list: D020932:Nerve Growth Factor