title: Immunomodulating activity of Pidotimod in children with Down syndrome.
abstract: Acute respiratory tract infections (ARTIs) are the most frequent illnesses in pediatric age, frequently experienced in children with Down Syndrome (DS) due to the associated immune defects of both specific and non-specific immunity. Pidotimod, a synthetic immunostimulant, was shown to reduce the rates of ARTIs in children with DS, however the mechanisms associated with this effect is currently unknown. We analyzed immune parameters in DS children who received the seasonal 20112012 virosomal-adjuvanted influenza vaccine. Eighteen children aged 3-10 years (mean age 7.1+/-2.6 years) were randomly assigned (1:1 ratio) to receive Pidotimod 400 mg, administered orally once a day for 90 days or placebo. At the recruitment (T0) all children received a single dose of virosomal-adjuvanted influenza vaccine (Flu). Blood samples were collected at T0 and 3 months after the recruitment (T3) in order to evaluate innate and adaptative immune responses pathway. Flu-specific IgG1 and IgG3 levels in plasma samples were determined at pre-vaccination (T0), and 1 (T1) and 3 months (T3) post-vaccination. The use of Pidotimod was associated with the upregulation of a number of genes involved in the activation of innate immune responses and in antimicrobial activity. Interestingly the ratio of Flu-specific IgG1/IgG3 was skewed in pidotimod-treated individuals, suggesting a preferential activation of complement-dependent effector mechanisms. Although preliminary these data suggest that Pidotimod can potentiate the beneficial effect of immunization, possibly resulting in a stronger activity of both innate and adaptive immune responses.
authors.forename: G V
authors.initials: GV
authors.lastname: Zuccotti
authors.forename: C
authors.initials: C
authors.lastname: Mameli
authors.forename: D
authors.initials: D
authors.lastname: Trabattoni
authors.forename: S
authors.initials: S
authors.lastname: Beretta
authors.forename: M
authors.initials: M
authors.lastname: Biasin
authors.forename: L
authors.initials: L
authors.lastname: Guazzarotti
authors.forename: M
authors.initials: M
authors.lastname: Clerici
mesh_terms: D056704:Adaptive Immunity / Q000187:drug effects / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004314:Down Syndrome / Q000097:blood / Q000188:drug therapy* / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use*; D007252:Influenza Vaccines / Q000276:immunology; D008297:Male; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use; D053778:Thiazolidines / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use*; D022721:Vaccines, Virosome / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D007155:Immunologic Factors; D007252:Influenza Vaccines; D053778:Thiazolidines; D022721:Vaccines, Virosome; C064893:pidotimod; D011761:Pyrrolidonecarboxylic Acid