title: Expression profiles of oncomir miR-21 and tumor suppressor let-7a in the progression of opisthorchiasis-associated cholangiocarcinoma.
abstract: Altered miRNA expression could be a determinant of cancer development and/or progression. We aimed to study the role of oncomir miR-21 and tumor suppressor let-7a in the genesis of Opisthorchiasis-associated cholangiocarcinoma (CCA). The results showed that miR-21 was up-regulated while let-7a was down-regulated during cholangiocarcinogenesis in the hamster model and also in human CCA samples. The expression level of miR-21 had an inverse correlation with the mRNA level of its target RECK, a metastasis suppressor, in human CCA. Knockdown of miR-21 of KKU100 CCA cells significantly increased the mRNA level of RECK and suppressed the wound-induced migration of CCA cells. Our data suggest that miR-21 is one key molecule playing crucial roles in the CCA growth and metastasis. Manipulation of miRNA expression offers a potential avenue of CCA therapy.
authors.affiliation: Department of Biochemistry, Khon Kean University, Khon Kaen, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Namwat
authors.forename: P
authors.initials: P
authors.lastname: Chusorn
authors.forename: W
authors.initials: W
authors.lastname: Loilome
authors.forename: A
authors.initials: A
authors.lastname: Techasen
authors.forename: J
authors.initials: J
authors.lastname: Puetkasichonpasutha
authors.forename: C
authors.initials: C
authors.lastname: Pairojkul
authors.forename: N
authors.initials: N
authors.lastname: Khuntikeo
authors.forename: P
authors.initials: P
authors.lastname: Yongvanit
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D009889:Opisthorchiasis / Q000235:genetics
chemical_list: D035683:MicroRNAs