title: A study of Caenorhabditis elegans DAF-2 mutants by metabolomics and differential correlation networks.
abstract: daf-2 is one of the most studied mutants in C. elegans: it contains a deletion in the gene orthologue of the insulin/insulin-like growth factor (IGF) receptor. Using high resolution (1)H NMR spectroscopy, metabolomics has helped to dissect the metabolic consequences of altered daf-2 signalling. Here, we present a detailed metabolomic analysis of daf-2, using NMR spectroscopy, gas chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) to integrate information from different pathways. We have then used Pearson and partial correlation analysis to build networks to explore the central role of daf-2 in regulating fatty acid and amino acid metabolism. The results show the tight regulation between these two parts of the metabolome.
authors.affiliation: Department of Biochemistry and the Cambridge Systems Biology Centre, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Castro
authors.forename: Jan
authors.initials: J
authors.lastname: Krumsiek
authors.forename: Nicolas J
authors.initials: NJ
authors.lastname: Lehrbach
authors.forename: Steven A
authors.initials: SA
authors.lastname: Murfitt
authors.forename: Eric A
authors.initials: EA
authors.lastname: Miska
authors.forename: Julian L
authors.initials: JL
authors.lastname: Griffin
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods; D050505:Mutant Proteins; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013696:Temperature
chemical_list: D000596:Amino Acids; D029742:Caenorhabditis elegans Proteins; D005227:Fatty Acids; D050505:Mutant Proteins; C107483:DAF-2 protein, C elegans; D011972:Receptor, Insulin