title: Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study.
abstract: In recent years, epigenetic markers emerged as a new tool to understand the influence of lifestyle factors on obesity phenotypes. Adolescence is considered an important epigenetic window over a human's lifetime. The objective of this work was to explore baseline changes in DNA methylation that could be associated with a better weight loss response after a multidisciplinary intervention program in Spanish obese or overweight adolescents. Overweight or obese adolescents (n=107) undergoing 10 wk of a multidisciplinary intervention for weight loss were assigned as high or low responders to the treatment. A methylation microarray was performed to search for baseline epigenetic differences between the 2 groups (12 subjects/group), and MALDI-TOF mass spectrometry was used to validate (n=107) relevant CpG sites and surrounding regions. After validation, 5 regions located in or near AQP9, DUSP22, HIPK3, TNNT1, and TNNI3 genes showed differential methylation levels between high and low responders to the multidisciplinary weight loss intervention. Moreover, a calculated methylation score was significantly associated with changes in weight, BMI-SDS, and body fat mass loss after the treatment. In summary, we have identified 5 DNA regions that are differentially methylated depending on weight loss response. These methylation changes may help to better understand the weight loss response in obese adolescents.
authors.affiliation: Department of Nutrition, Food Science, Physiology, and Toxicology, University of Navarra, Pamplona, Spain.
authors.forename: Adriana
authors.initials: A
authors.lastname: Moleres
authors.forename: Javier
authors.initials: J
authors.lastname: Campión
authors.forename: Fermín I
authors.initials: FI
authors.lastname: Milagro
authors.forename: Ascensión
authors.initials: A
authors.lastname: Marcos
authors.forename: Cristina
authors.initials: C
authors.lastname: Campoy
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Garagorri
authors.forename: Sonia
authors.initials: S
authors.lastname: Gómez-Martínez
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martínez
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Azcona-Sanjulián
authors.forename: Amelia
authors.initials: A
authors.lastname: Martí
mesh_terms: D000293:Adolescent; D020346:Aquaporins / Q000235:genetics; D019175:DNA Methylation; D054637:Dual-Specificity Phosphatases / Q000235:genetics; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008297:Male; D054639:Mitogen-Activated Protein Kinase Phosphatases / Q000235:genetics; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D050177:Overweight / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D013030:Spain; D019210:Troponin I / Q000235:genetics; D020107:Troponin T / Q000235:genetics; D015431:Weight Loss / Q000235:genetics* / Q000502:physiology; D061217:Weight Reduction Programs
chemical_list: C109669:AQP9 protein, human; D020346:Aquaporins; D047908:Intracellular Signaling Peptides and Proteins; D019210:Troponin I; D020107:Troponin T; C109102:HIPK3 protein, human; D017346:Protein Serine-Threonine Kinases; D054639:Mitogen-Activated Protein Kinase Phosphatases; C442824:DUSP22 protein, human; D054637:Dual-Specificity Phosphatases
keywords: biomarkers; dieting response; epigenetics