title: A-kinase anchoring proteins 10 expression in relation to 2073A/G polymorphism and tumor progression in patients with colorectal cancer.
abstract: The cAMP/PKA signalling events regulated by A-kinase anchoring proteins 10 (AKAP10) is involved in tumorigenesis. Previous study showed that AKAP10 polymorphism (2073 A/G, I646V) was associated with colorectal cancer risk. However, there was no literature reporting the role of AKAP10 in the pathogenesis of colorectal cancer. The aim of the study was to investigate the clinicopathologic significance of A-kinase anchoring proteins 10 (AKAP 10) expression and the relationship with its polymorphism in colorectal cancer. The expression of AKAP10 was determined by immunohistochemical staining (IHC) and western blot assay on colorectal cancer (n = 176), adenoma (n = 87) and distant normal mucosa (n = 72). 176 patients with colorectal cancer were genotyped for AKAP10 2073A/G polymorphism by TaqMan RT-PCR. We found that the positive expression rate of AKAP10 in colorectal cancer (59 %) was significantly higher than those in adenoma (39 %) and distant normal mucosa (42 %) (P = 0.004). There was no significant difference between adenoma and distant normal mucosa (P = 0.741). Positive AKAP10 staining was correlated with deeper tumor invasion (P < 0.001), lymph nodes metastasis (P = 0.022), advanced tumor stage (P < 0.001) and poorly differentiated degree (P = 0.003). Compared with AA genotype (52 %), positive expression of AKAP10 was significantly increased in colorectal cancer patients with the variant (AG+GG) genotypes (68 %, P = 0.033). It was concluded that AKAP10 may play an important role in the development and progression of colorectal cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Institute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu 610041, China.
authors.forename: Mojin
authors.initials: M
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Yuanyi
authors.initials: Y
authors.lastname: Rui
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Huang
authors.forename: Lie
authors.initials: L
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Jiankun
authors.initials: J
authors.lastname: Hu
authors.forename: Zongguang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Sun
mesh_terms: D054758:A Kinase Anchor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016009:Chi-Square Distribution; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D054758:A Kinase Anchor Proteins; C484639:AKAP10 protein, human