title: Establishment of a lentiviral vector encoding human HGF and the infection of human ADSCs.
abstract: The delivery of adipose-derived stem cells (ADSCs) for promoting tissue repair has become a potential new therapy, while hepatocyte growth factor (HGF) is an important growth factor with angiogenic, anti-fibrotic, and anti-inflammatory benefits. In this paper, hADSCs were separated, cultured and identified based on the expression of cell surface antigens and multiple differentiation potential. We successfully generated a lentiviral vector encoding human HGF, infected hADSCs with this vector and examined the protein expression pattern. Finally we found that the hHGF lentiviral vector was successfully generated, and the lentiviral vector was able to safely infect hADSCs with high infection efficiency, thereby producing cells that overexpressed hHGF, which may provide a new strategy for the treatment of ischemic heart disease (IHD) and other ischemic diseases.
authors.affiliation: Department of Hematology, Anhui Provincial Hospital of Anhui Medical University, Hefei 23001, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Wu
authors.forename: Weibo
authors.initials: W
authors.lastname: Zhu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cai
authors.forename: Zimin
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D016086:Lentivirus; D008297:Male; D017202:Myocardial Ischemia / Q000628:therapy; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: C066855:HGF protein, human; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor