title: Yeast proteome variations reveal different adaptive responses to grape must fermentation.
abstract: Saccharomyces cerevisiae and S. uvarum are two domesticated species of the Saccharomyces sensu stricto clade that diverged around 100 Ma after whole-genome duplication. Both have retained many duplicated genes associated with glucose fermentation and are characterized by the ability to achieve grape must fermentation. Nevertheless, these two species differ for many other traits, indicating that they underwent different evolutionary histories. To determine how the evolutionary histories of S. cerevisiae and S. uvarum are mirrored on the proteome, we analyzed the genetic variability of the proteomes of domesticated strains of these two species by quantitative mass spectrometry. Overall, 445 proteins were quantified. Massive variations of protein abundances were found, that clearly differentiated the two species. Abundance variations in specific metabolic pathways could be related to phenotypic traits known to discriminate the two species. In addition, proteins encoded by duplicated genes were shown to be differently recruited in each species. Comparing the strain differentiation based on the proteome variability to those based on the phenotypic and genetic variations further revealed that the strains of S. uvarum and some strains of S. cerevisiae displayed similar fermentative performances despite strong proteomic and genomic differences. Altogether, these results indicate that the ability of S. cerevisae and S. uvarum to complete grape must fermentation arose through different evolutionary roads, involving different metabolic pathways and duplicated genes.
authors.affiliation: INRA, PAPPSO, UMR 0320/8120 Génétique Végétale, Gif sur Yvette, France.
authors.forename: Mélisande
authors.initials: M
authors.lastname: Blein-Nicolas
authors.forename: Warren
authors.initials: W
authors.lastname: Albertin
authors.forename: Benoît
authors.initials: B
authors.lastname: Valot
authors.forename: Philippe
authors.initials: P
authors.lastname: Marullo
authors.forename: Delphine
authors.initials: D
authors.lastname: Sicard
authors.forename: Christophe
authors.initials: C
authors.lastname: Giraud
authors.forename: Sylvie
authors.initials: S
authors.lastname: Huet
authors.forename: Aurélie
authors.initials: A
authors.lastname: Bourgais
authors.forename: Christine
authors.initials: C
authors.lastname: Dillmann
authors.forename: Dominique
authors.initials: D
authors.lastname: de Vienne
authors.forename: Michel
authors.initials: M
authors.lastname: Zivy
mesh_terms: D016000:Cluster Analysis; D019143:Evolution, Molecular; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020440:Gene Duplication / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D010449:Peptide Mapping; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012440:Saccharomyces / Q000235:genetics / Q000378:metabolism*; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins; D020543:Proteome; D005947:Glucose
keywords: adaptive evolution; genetic diversity; genome duplication; glucose fermentation; shotgun proteomics