title: Parallel real-time PCR on a chip for genetic tug-of-war (gTOW) method.
abstract: A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote Saccharomyces cerevisiae were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan. naito.toyohiro@f.mbox.nagoya-u.ac.jp
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Naito
authors.forename: Ai
authors.initials: A
authors.lastname: Yatsuhashi
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.forename: Taeko
authors.initials: T
authors.lastname: Ando
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sato
authors.forename: Hisao
authors.initials: H
authors.lastname: Moriya
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kitano
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013696:Temperature