title: Preparation of recombinant atoxic form of exotoxin A from Pseudomonas aeruginosa.
abstract: Nucleotide sequences encoding full-length protein of Pseudomonas aeruginosa exotoxin A and its atoxic form were cloned and expressed in Escherichia coli cells. Purified recombinant exotoxin and immune rabbit sera protected mice from exotoxin A.
authors.affiliation: I. I. Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia. alex-k-1973@yandex.ru
authors.forename: A A
authors.initials: AA
authors.lastname: Kaloshin
authors.forename: M A
authors.initials: MA
authors.lastname: Isakov
authors.forename: N A
authors.initials: NA
authors.lastname: Mikhailova
authors.forename: Ju V
authors.initials: JV
authors.lastname: Vertiev
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D003001:Cloning, Molecular; D005098:Exotoxins / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D007106:Immune Sera; D007114:Immunization; D051379:Mice; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000633:toxicity; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D007106:Immune Sera; D011994:Recombinant Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A