title: Simultaneous analysis of guaiacol and vanillin in a vanilla extract by using high-performance liquid chromatography with electrochemical detection.
abstract: We developed and validated a new high-performance liquid chromatographic analysis for electrochemically detecting guaiacol and vanillin as important components in vanilla extract. Separation was achieved with Capcell Pak C-18 MG, the potential of the working electrode being set at +1000 mV. The respective calibration curves for guaiacol and vanillin were linear in the range of 1.60-460 µg/L and 5.90-1180 µg/L. The respective limits for the quantities of guaiacol and vanillin were 1.60 µg/L and 2.36 µg/L. The related standard deviations of the intra- and inter-day precision of the retention time and peak area were all less than 4%. The recovery of guaiacol and vanillin was both more than 97%, all of the validation data being within an acceptable range. This analysis method is well suited for the simultaneous and convenient analysis of guaiacol and vanillin in a vanilla extract to evaluate the quality of the vanilla extract.
authors.affiliation: Technical Research Institute, R&D Center, T. Hasegawa Co., Ltd., Kawasaki, Kanagawa, Japan. makoto_takahashi@t-hasegawa.co.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Takahashi
authors.forename: Shizuka
authors.initials: S
authors.lastname: Sakamaki
authors.forename: Akira
authors.initials: A
authors.lastname: Fujita
mesh_terms: D001547:Benzaldehydes / Q000032:analysis* / Q000737:chemistry* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D063427:Food Quality; D006139:Guaiacol / Q000032:analysis* / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008297:Male; D009812:Odorants / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012677:Sensation / Q000187:drug effects; D013997:Time Factors; D031669:Vanilla / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D010936:Plant Extracts; D006139:Guaiacol; C100058:vanillin