title: Characterization of glycoprotein biopharmaceutical products by Caliper LC90 CE-SDS gel technology.
abstract: Over the last decade, science has greatly improved in the area of protein sizing and characterization. Efficient high-throughput methods are now available to substitute for the traditional labor-intensive SDS-PAGE methods, which alternatively take days to analyze a very limited number of samples. Currently, PerkinElmer(®) (Caliper) has designed an automated chip-based fluorescence detection method capable of analyzing proteins in minutes with sensitivity similar to standard SDS-PAGE. Here, we describe the use and implementation of this technology to characterize and screen a large number of formulations of target glycoproteins in the 14-200 kDa molecular weight range.
authors.affiliation: Vaccine Analytical Development, Merck Research Laboratories, West Point, PA, USA.
authors.forename: Grace
authors.initials: G
authors.lastname: Chen
authors.forename: Sha
authors.initials: S
authors.lastname: Ha
authors.forename: Richard R
authors.initials: RR
authors.lastname: Rustandi
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000592:standards; D004921:Erythropoietin / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods / Q000592:standards; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012015:Reference Standards; D013050:Spectrometry, Fluorescence
chemical_list: D006023:Glycoproteins; D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins; D004921:Erythropoietin; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase