title: icb-1 Gene counteracts growth of ovarian cancer cell lines.
abstract: Human gene icb-1 has been originally identified to be involved in differentiation processes of cancer cells. To examine the function of icb-1 in ovarian cancer, we knocked down its expression in three ovarian cancer cell lines and performed microarray-based gene expression profiling with subsequent gene network modeling. Loss of icb-1 expression accelerated proliferation of SK-OV-3, OVCAR-3 and OAW-42 cells and led to upregulation of ovarian cancer biomarkers like KLK10 and CLDN16. Most of the upregulated genes were part of oncogenic pathways regulated by ERα or TNF. Our data suggest that icb-1 gene inhibits growth and progression of ovarian cancer cells.
authors.affiliation: Department of Obstetrics and Gynecology, University Medical Center Regensburg, Regensburg, Germany. otreeck@caritasstjosef.de
authors.forename: Oliver
authors.initials: O
authors.lastname: Treeck
authors.forename: Susanne
authors.initials: S
authors.lastname: Schüler
authors.forename: Julia
authors.initials: J
authors.lastname: Häring
authors.forename: Maciej
authors.initials: M
authors.lastname: Skrzypczak
authors.forename: Claus
authors.initials: C
authors.lastname: Lattrich
authors.forename: Olaf
authors.initials: O
authors.lastname: Ortmann
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D057167:Claudins / Q000096:biosynthesis; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007610:Kallikreins / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D057167:Claudins; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C111850:THEMIS2 protein, human; D014409:Tumor Necrosis Factor-alpha; C502010:claudin 16; C100936:KLK10 protein, human; D007610:Kallikreins