title: The role of viral hemorrhagic septicemia virus (VHSV) NV gene in TNF-α- and VHSV infection-mediated NF-κB activation.
abstract: The role of viral hemorrhagic septicemia virus (VHSV) NV gene in nuclear factor-κB (NF-κB) activation was investigated. Epithelioma papulosum cyprini (EPC) cells pre-treated with tumor necrosis factor (TNF)-α showed a strong resistance against VHSV infection, but cells treated with TNF-α after VHSV infection showed no resistance, suggesting that immediate early TNF-α-mediated responses inhibit VHSV replication. Activation of NF-κB is a key step in TNF-α-mediated immunomodulatory pathways. In this study, activation of NF-κB by TNF-α exposure was inhibited in EPC cells harboring NV gene expressing vectors, indicating that the NV gene of VHSV can suppress TNF-α-mediated NF-κB activation. Furthermore, the NV gene knock-out recombinant VHSV (rVHSV-ΔNV-EGFP) induced significantly higher NF-κB activity in EPC cells than wild-type VHSV, suggesting that VHSV adopted a strategy to suppress early activation of NF-κB in host cells through and NV gene.
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Nam-gu 599-1, Busan 608-737, Republic of Korea.
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Ki Hong
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002347:Carps; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D055786:Gene Knockout Techniques; D016328:NF-kappa B / Q000378:metabolism*; D029141:Novirhabdovirus / Q000235:genetics* / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins