title: [Improvement of carbon tetrachloride drug-induced liver injury model in vitro].
abstract: OBJECTIVE
  To establish an ideal CCl4 drug-induced liver injury model in vitro.
  
  
  METHODS
  Traditional method and improved method were adopted for preparing CCl4 injury liquid and drug-induced human liver HepG2 cell injury. Cell morphological change was observed under a bright-field microscope. The level of alanine aminotransferase (ALT) in supernatant was detected by biochemical method. 4-Methyl-tetrazolium (MTT) chromatometry was adopted for determining cell activity.
  
  
  RESULTS
  The improved method showed better CCl4-induced injury effect than the traditional method. With the increase in the concentration of CCl4 injury liquid, the ALT level significantly increased, whereas the cell activity notably decreased. Particularly, 70% CCl4 injury liquid use for 4 hours could achieve the best injury effect.
  
  
  CONCLUSIONS
  The improved method could be used to establish an ideal CCl4 drug-induced liver injury model in vitro, which can lay foundation for further in vitro studies.
authors.affiliation: Cancer Center, Qilu Hospital, Shandong University, Ji'nan 250012, China. wuhaijian2009@126.com
authors.forename: Hai-Jian
authors.initials: HJ
authors.lastname: Wu
authors.forename: Xiu
authors.initials: X
authors.lastname: Gong
authors.forename: Yi-Tian
authors.initials: YT
authors.lastname: Yang
authors.forename: Yu-Zhuo
authors.initials: YZ
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Xin
authors.forename: Li-Kuan
authors.initials: LK
authors.lastname: Hu
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000009:adverse effects*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000209:etiology*; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000293:injuries; D008954:Models, Biological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase