title: Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.
abstract: Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the APC/C(Cdh1) complex, but not by the APC/C(Cdc20) complex, and is phosphorylated by Cdk1 in mitosis. Moreover, late in cytokinesis, Gas2l3 is exclusively localized to the constriction sites, which are the narrowest parts of the intercellular bridge connecting the two daughter cells. Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs. We therefore suggest that Gas2l3 is part of the cellular mechanism that terminates cell division.
authors.affiliation: Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Tal
authors.initials: T
authors.lastname: Pe'er
authors.forename: Roxane
authors.initials: R
authors.lastname: Lahmi
authors.forename: Yaara
authors.initials: Y
authors.lastname: Sharaby
authors.forename: Evelin
authors.initials: E
authors.lastname: Chorni
authors.forename: Meirav
authors.initials: M
authors.lastname: Noach
authors.forename: Manuela
authors.initials: M
authors.lastname: Vecsler
authors.forename: Eitan
authors.initials: E
authors.lastname: Zlotorynski
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.forename: Judith A
authors.initials: JA
authors.lastname: Steen
authors.forename: Amit
authors.initials: A
authors.lastname: Tzur
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D002453:Cell Cycle; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D014404:Tubulin / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D000199:Actins; D015703:Antigens, CD; D015820:Cadherins; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin; C513384:CDH1 protein, human; C569298:GAS2L3 protein, human