title: Role of peptide YY(3-36) in the satiety produced by gastric delivery of macronutrients in rats.
abstract: Peptide YY(3-36) [PYY(3-36)] is postulated to act as a hormonal signal from gut to brain to inhibit food intake. PYY(3-36) potently reduces food intake when administered systemically or into the brain. If action of endogenous PYY(3-36) is necessary for normal satiation to occur, then pharmacological blockade of its receptors should increase food intake. Here, we determined the effects of iv infusion of Y1, Y2, and Y5 receptor antagonists (BIBP 3226, BIIE 0246, CGP 71683) during the first 3 h of the dark period on food intake in non-food-deprived rats. Our results showed that 1) Y2 receptor blockade reversed the anorexic response to iv infusion of PYY(3-36) but did not increase food intake when administered alone; 2) Y1 and Y5 receptor antagonists neither attenuated PYY(3-36)-induced anorexia nor altered food intake when given alone; and 3) Y2 receptor blockade attenuated anorexic responses to gastric infusions of casein hydrolysate and long-chain triglycerides, but not maltodextrin. Previous work showed that Y2 antagonist BIIE 0246 does not penetrate the blood-brain barrier. Together, these results support the hypothesis that gut PYY(3-36) action at Y2 receptors peripheral to the blood brain barrier plays an essential role in mediating satiety responses to gastric delivery of protein and long-chain triglycerides, but not polysaccharide.
authors.affiliation: Veterans Affairs Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA.
authors.forename: Roger
authors.initials: R
authors.lastname: Reidelberger
authors.forename: Alvin
authors.initials: A
authors.lastname: Haver
authors.forename: Prasanth K
authors.initials: PK
authors.lastname: Chelikani
mesh_terms: D000818:Animals; D000855:Anorexia / Q000523:psychology; D002766:Cholecystokinin / Q000502:physiology; D004435:Eating / Q000187:drug effects; D006815:Hunger / Q000502:physiology; D007441:Intubation, Gastrointestinal; D058228:Islet Amyloid Polypeptide / Q000502:physiology; D008297:Male; D010446:Peptide Fragments / Q000502:physiology*; D019894:Peptide YY / Q000502:physiology*; D011134:Polysaccharides / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011964:Receptors, Gastrointestinal Hormone / Q000037:antagonists & inhibitors; D012528:Satiety Response / Q000502:physiology*; D014280:Triglycerides / Q000494:pharmacology
chemical_list: D058228:Islet Amyloid Polypeptide; D010446:Peptide Fragments; D011134:Polysaccharides; D011492:Protein Hydrolysates; D011964:Receptors, Gastrointestinal Hormone; D014280:Triglycerides; C082172:peptide YY receptor; D019894:Peptide YY; C061785:peptide YY (3-36); C008315:maltodextrin; D002766:Cholecystokinin
keywords: BIIE 0246; PYY; Y receptor antagonists; carbohydrate; food intake; lipid; protein