title: The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5.
abstract: BACKGROUND
  Bacteriophages (phages) have been used extensively as analytical tools to type bacterial cultures and recently for control of zoonotic foodborne pathogens in foods and in animal reservoirs.
  
  
  METHODS
  We examined the host range, morphology, genome and proteome of the lytic E. coli O157 phage rV5, derived from phage V5, which is a member of an Escherichia coli O157:H7 phage typing set.
  
  
  RESULTS
  Phage rV5 is a member of the Myoviridae family possessing an icosahedral head of 91 nm between opposite apices. The extended tail measures 121 x 17 nm and has a sheath of 44 x 20 nm and a 7 nm-wide core in the contracted state. It possesses a 137,947 bp genome (43.6 mol%GC) which encodes 233 ORFs and six tRNAs. Until recently this virus appeared to be phylogenetically isolated with almost 70% of its gene products ORFans. rV5 is closely related to coliphages Delta and vB-EcoM-FY3, and more distantly related to Salmonella phages PVP-SE1 and SSE-121, Cronobacter sakazakii phage vB_CsaM_GAP31, and coliphages phAPEC8 and phi92. A complete shotgun proteomic analysis was carried out on rV5, extending what had been gleaned from the genomic analyses. Host range studies revealed that rV5 is active against several other E. coli.
authors.affiliation: Public Health Agency of Canada, Laboratory for Foodborne Diseases, 110 Stone Road West, Guelph, ON N1G 3W4, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: Tom
authors.initials: T
authors.lastname: Waddell
authors.forename: Juncai
authors.initials: J
authors.lastname: Meng
authors.forename: Kristyn
authors.initials: K
authors.lastname: Franklin
authors.forename: Hans-Wolfgang
authors.initials: HW
authors.lastname: Ackermann
authors.forename: Rafiq
authors.initials: R
authors.lastname: Ahmed
authors.forename: Amanda
authors.initials: A
authors.lastname: Mazzocco
authors.forename: John
authors.initials: J
authors.lastname: Yates
authors.forename: Erika J
authors.initials: EJ
authors.lastname: Lingohr
authors.forename: Roger P
authors.initials: RP
authors.lastname: Johnson
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D019453:Escherichia coli O157 / Q000821:virology*; D016679:Genome, Viral; D023281:Genomics; D058507:Host Specificity; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016366:Open Reading Frames; D010802:Phylogeny; D040901:Proteomics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins