title: Cell-laden poly(ɛ-caprolactone)/alginate hybrid scaffolds fabricated by an aerosol cross-linking process for obtaining homogeneous cell distribution: fabrication, seeding efficiency, and cell proliferation and distribution.
abstract: Generally, solid-freeform fabricated scaffolds show a controllable pore structure (pore size, porosity, pore connectivity, and permeability) and mechanical properties by using computer-aided techniques. Although the scaffolds can provide repeated and appropriate pore structures for tissue regeneration, they have a low biological activity, such as low cell-seeding efficiency and nonuniform cell density in the scaffold interior after a long culture period, due to a large pore size and completely open pores. Here we fabricated three different poly(ɛ-caprolactone) (PCL)/alginate scaffolds: (1) a rapid prototyped porous PCL scaffold coated with an alginate, (2) the same PCL scaffold coated with a mixture of alginate and cells, and (3) a multidispensed hybrid PCL/alginate scaffold embedded with cell-laden alginate struts. The three scaffolds had similar micropore structures (pore size = 430-580 μm, porosity = 62%-68%, square pore shape). Preosteoblast cells (MC3T3-E1) were used at the same cell density in each scaffold. By measuring cell-seeding efficiency, cell viability, and cell distribution after various periods of culturing, we sought to determine which scaffold was more appropriate for homogeneously regenerated tissues.
authors.affiliation: Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea .
authors.forename: HyeongJin
authors.initials: H
authors.lastname: Lee
authors.forename: SeungHyun
authors.initials: S
authors.lastname: Ahn
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Bonassar
authors.forename: Wook
authors.initials: W
authors.lastname: Chun
authors.forename: GeunHyung
authors.initials: G
authors.lastname: Kim
mesh_terms: D000336:Aerosols / Q000494:pharmacology*; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D061848:Optical Imaging; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000336:Aerosols; D000464:Alginates; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D011091:Polyesters; C016240:polycaprolactone; D020723:Glucuronic Acid