title: Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells.
abstract: OBJECTIVE
  To investigate the new tissues growing into the pulp space of immature dog teeth that were infected, disinfected and filled with blood clot (BC), dental pulp cells (DPCs), platelet-rich plasma (PRP) or a combination of DPCs and PRP in immature dog teeth with apical periodontitis.
  
  
  METHODS
  Fifty-six immature roots from mandibular premolars of four beagles were divided into four experimental groups (n = 40) and two control groups. After the induction of apical periodontitis, the root canals of experimental groups were disinfected with NaOCl irrigation and a tri-antibiotic paste medication. The canals were then filled with different materials according to the experimental group: BC group, DPCs group, PRP group or DPCs + PRP group. Access cavities were sealed with MTA and composite. Radiographs were taken after 90 days, and the jaws including the teeth were processed for histologic analysis. The data were statistically analysed using chi-square evaluation and Student's t-test.
  
  
  RESULTS
  Radiographic analyses demonstrated no significant difference between experimental groups in periradicular bone healing (P > 0.05), whilst those groups that used DPCs produced a significantly greater root thickening (P < 0.01). The histologic evaluation showed that the groups with PRP formed more tissues in the canals (P = 0.01). The groups with DPCs had substantially more mineralized tissue formation in the canal than those without DPCs, especially in the apical third. In DPCs + PRP group, bone-like tissue grew into the canal space from the periapical tissue.
  
  
  CONCLUSIONS
  A combination of DPCs + PRP increased vital tissue regeneration within the root canals of immature teeth associated with apical periodontitis.
authors.affiliation: Department of General Dentistry, School and Hospital of Stomatology, Peking University, Beijing, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhu
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: G T-J
authors.initials: GT
authors.lastname: Huang
authors.forename: G S P
authors.initials: GS
authors.lastname: Cheung
authors.forename: W L
authors.initials: WL
authors.lastname: Dissanayaka
authors.forename: C
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003782:Dental Pulp / Q000473:pathology*; D004285:Dogs; D008297:Male; D010485:Periapical Periodontitis / Q000473:pathology*; D053657:Platelet-Rich Plasma; D012038:Regeneration; D012387:Root Canal Filling Materials
chemical_list: D012387:Root Canal Filling Materials
keywords: dental pulp cells; immature teeth; platelet-rich plasma; regenerative endodontics