title: Single nucleotide polymorphism analysis of a Trichoderma reesei hyper-cellulolytic mutant developed in Japan.
abstract: The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of single-nucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan.
authors.forename: Juliano de Oliveira
authors.initials: Jde O
authors.lastname: Porciuncula
authors.forename: Takanori
authors.initials: T
authors.lastname: Furukawa
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mori
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Shida
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Morikawa
authors.forename: Wataru
authors.initials: W
authors.lastname: Ogasawara
mesh_terms: D000595:Amino Acid Sequence; D002480:Cellulase / Q000096:biosynthesis; D002482:Cellulose / Q000378:metabolism*; D004271:DNA, Fungal / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D007564:Japan; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D017434:Protein Structure, Tertiary; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D002482:Cellulose; D002480:Cellulase; D005947:Glucose