title: Comparison of the Abbott m2000 RealTime CT assay and the Cepheid GeneXpert CT/NG assay to the Roche Amplicor CT assay for detection of Chlamydia trachomatis in ocular samples from Tanzania.
abstract: The GeneXpert CT/NG assay (GeneXpert) and the Abbott m2000 RealTime CT (m2000) assay were compared to Amplicor for detecting ocular Chlamydia trachomatis. Discordant specimens were tested by the Aptima CT assay. The m2000 assay sensitivity was 100% (95% confidence interval [CI], 90% to 100%), and specificity was 98.46% (95% CI, 95.2% to 99.2%); GeneXpert sensitivity was 100% (95% CI, 90% to 100%), and specificity was 100% (95% CI, 98.1% to 100%). The m2000 and GeneXpert assays appear to perform as well as the Amplicor assay.
authors.affiliation: Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Dize
authors.forename: Sheila
authors.initials: S
authors.lastname: West
authors.forename: James A
authors.initials: JA
authors.lastname: Williams
authors.forename: Barbara
authors.initials: B
authors.lastname: Van Der Pol
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Quinn
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Gaydos
mesh_terms: D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013636:Tanzania; D014141:Trachoma / Q000175:diagnosis*
chemical_list: D011933:Reagent Kits, Diagnostic