title: The KRAS StripAssay for detection of KRAS mutation in Egyptian patients with colorectal cancer (CRC): a pilot study.
abstract: BACKGROUND
  Epidermal growth factor receptor (EGFR) and its downstream factors KRAS and BRAF are mutated in several types of cancer, affecting the clinical response to EGFR inhibitors. Mutations in the EGFR kinase domain predict sensitivity to the tyrosine kinase inhibitors gefitinib and erlotinib in lung adenocarcinoma, while activating point mutations in KRAS and BRAF confer resistance to the anti-EGFR monoclonal antibody cetuximab in colorectal cancer. The development of new generation methods for systematic mutation screening of these genes will allow more appropriate therapeutic choices.
  
  
  OBJECTIVE
  Detection of KRAS mutation in Egyptian colorectal cancer (CRC) patients by the KRAS StripAssay.
  
  
  METHODS
  Examination of 20 colorectal cancer (CRC) patients is done to detect KRAS mutations by KRAS StripAssay. For the StripAssay, a mutant-enriched PCR was followed by hybridization to KRAS-specific probes bound to a nitrocellulose strip.
  
  
  RESULTS
  Among 20 patients, KRAS mutations were identified in 80% of patients by the KRAS StripAssay.
  
  
  CONCLUSIONS
  Our preliminary results suggest that KRAS StripAssay is an alternative to protocols currently in use for KRAS mutation detection.
authors.affiliation: Kasr El-Aini Centre of Clinical Oncology and Nuclear Medicine (NEMROCK), Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Yasser
authors.initials: Y
authors.lastname: Abd El Kader
authors.forename: Gamal
authors.initials: G
authors.lastname: Emera
authors.forename: Ezzat
authors.initials: E
authors.lastname: Safwat
authors.forename: Heba A
authors.initials: HA
authors.lastname: Kassem
authors.forename: Neemat M
authors.initials: NM
authors.lastname: Kassem
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics*; D000368:Aged; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D004534:Egypt; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins / Q000235:genetics*
chemical_list: C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins