title: In vitro mineralization of hydroxyapatite on electrospun poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) fibrous scaffolds for tissue engineering application.
abstract: In this study, a fibrous scaffold was prepared by electrospinning triblock PCL-PEG-PCL (PCEC) copolymer. Afterwards, in vitro biomimetic mineralization was carried out through incubation of the PCEC fibrous mats in a simulated body fluid (SBF) for different time. The apatite-deposited PCEC composite scaffolds were characterized by using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscope (SEM) observation and weighing. Due to the importance of biocompatibility, rat ROS 17/2.8 osteoblasts were cultured on mineralized PCEC scaffolds, and the cell proliferation was investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assays. The obtained results confirmed that the deposited apatite had the chemical composition and crystalline phase similar to those of hydroxyapatite (HA). After 21 days incubation, the mass increase of PCEC scaffold reached up to 22%. Moreover, in vitro cell culture also confirmed that osteoblasts could attach on the mineralized composite scaffolds, and the HA-deposited PCEC mats had less cytotoxicity. So, the mineralized PCEC composite scaffolds had a great potential for tissue engineering application.
authors.affiliation: Department of Oncology, The Affiliated Hospital of Luzhou Medical College, Luzhou 646000, PR China. shaozhifu513@163.com
authors.forename: ShaoZhi
authors.initials: S
authors.lastname: Fu
authors.forename: LingLin
authors.initials: L
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Fan
authors.forename: QingLian
authors.initials: Q
authors.lastname: Wen
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.forename: BiQiong
authors.initials: B
authors.lastname: Wang
authors.forename: LanLan
authors.initials: L
authors.lastname: Chen
authors.forename: XiaoHang
authors.initials: X
authors.lastname: Meng
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: JingBo
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D008903:Minerals / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D010758:Phosphorus / Q000032:analysis; D011091:Polyesters / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D008903:Minerals; D011091:Polyesters; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D010758:Phosphorus; D011092:Polyethylene Glycols; D017886:Durapatite; D002118:Calcium