title: In vivo detection of lipid-based nano- and microparticles in the outermost human stratum corneum by EDX analysis.
abstract: Lipid-based particulate delivery systems have been extensively investigated in the last decade for both pharmaceutical and cosmetic skin application although their translocation across the skin is not yet clarified. The aim of this paper was to investigate on humans the ability of solid lipid nanoparticles (SLN) and solid lipid microparticles (SLM) to penetrate the outermost stratum corneum (SC) and to be modified upon contact with the cutaneous components by using the Tape Stripping Test coupled with the energy dispersive X-ray (EDX) analysis. SLN and SLM were prepared by the melt emulsification technique and loaded with nanosized titanium dioxide (TiO2) to become identifiable by means of X-ray emission. Following human skin application, the translocation of the particulate systems was monitored by the analysis of twelve repetitive stripped tapes using non-encapsulated metal dioxide as the control. Intact SLN as well as non-encapsulated TiO₂ were recorded along the largest SC openings until the 12th stripped tape suggesting the intercluster region as their main pathway. Evidences of a concurrent biodegradation process of the lipid matrix, as the result of SLN interaction with the lipid packing between the corneocyte clusters, were found in the deepest SC layers considered. On the contrary, SLM were retained on the skin surface without undergoing biodegradation so preventing the leaching and the subsequent SC translocation of the loaded TiO₂.
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 183, 41125 Modena, Italy. valentina.iannuccelli@unimore.it
authors.forename: Valentina
authors.initials: V
authors.lastname: Iannuccelli
authors.forename: Gilberto
authors.initials: G
authors.lastname: Coppi
authors.forename: Marcello
authors.initials: M
authors.lastname: Romagnoli
authors.forename: Santo
authors.initials: S
authors.lastname: Sergi
authors.forename: Eliana
authors.initials: E
authors.lastname: Leo
mesh_terms: D000328:Adult; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D006801:Humans; D008055:Lipids / Q000008:administration & dosage* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D012869:Skin Absorption; D013052:Spectrometry, X-Ray Emission; D014025:Titanium / Q000008:administration & dosage* / Q000737:chemistry; D055815:Young Adult
chemical_list: D008055:Lipids; C009495:titanium dioxide; D014025:Titanium