title: The interleukin-1β system in the corpora lutea of pigs during early pregnancy and the estrous cycle.
abstract: Expression of mRNAs encoding interleukin-1β (IL-1β), IL-1β receptor I (IL-1RI), IL-1 receptor accessory protein (IL-1RAcP) and IL-1 receptor antagonist (IL-1Ra), as well as synthesis of IL-1β and IL-1RI proteins, were examined in the corpus luteum (CL) during critical stages of CL activity on days 10-16 of pregnancy and 2-16 of the estrous cycle. Luteal cells were cultured in vitro with IL-1β, and the effect on release of steroid hormones was determined. Expression of the IL-1β system in the CL changed significantly during pregnancy and the estrous cycle. IL-1β, IL-1RI, and IL-1Ra mRNA levels were elevated on days 12-13, whereas IL-1RAcP mRNA was increased on days 15-16 of pregnancy. In cyclic CL, expression of IL-1β, IL-1RI, and IL-1RAcP mRNAs was increased on days 12-13. IL-1β and IL-1RI protein were highest in the CL on days 10-11 and 8-11 of pregnancy and the estrous cycle. Luteal cells harvested from gravid and cyclic CL produced IL-1β in vitro. IL-1β increased progesterone and estradiol-17β (E2) release by luteal cells on days 10-16 and 10-11 of pregnancy, respectively and on days 2-11 of the estrous cycle. IL-1β decreased the level of E2 produced by regressed CL (days 15-16). Expression of the IL-1β system in CL and IL-1β secretion from luteal cells changed depending on the status of the CL. These data show that IL-1β may be involved in intraluteal, luteotrophic regulation of CL functions in gravid and cyclic pigs.
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Zmijewska
authors.forename: Anita
authors.initials: A
authors.lastname: Franczak
authors.forename: Genowefa
authors.initials: G
authors.lastname: Kotwica
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology / Q000276:immunology*; D030762:Estrous Cycle / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics / Q000378:metabolism; D053577:Interleukin-1 Receptor Accessory Protein / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011270:Pregnancy, Animal / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000276:immunology / Q000502:physiology*
chemical_list: D012739:Gonadal Steroid Hormones; D053590:Interleukin 1 Receptor Antagonist Protein; D053577:Interleukin-1 Receptor Accessory Protein; D053583:Interleukin-1beta; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1