title: Intravaginal live attenuatedSalmonellaincrease local antitumor vaccine-specific CD8+T cells.
abstract: We have recently reported that the intravaginal instillation of synthetic Toll-like receptor 3 (TLR3) or TLR9 agonists after a subcutaneous vaccination against human papillomavirus E7 highly increases (~5-fold) the number of vaccine-specific CD8+T cells in the genital mucosa of mice, without affecting E7-specific systemic responses. Here, we show that the instillation of live attenuatedSalmonella entericaserovar Typhimurium similarly, though more efficiently (~15- fold), increases both E7-specific and total CD8+T cells in the genital mucosa. Cancer immunotherapeutic strategies combining vaccination with local immunostimulation with live bacteria deserve further investigations.
authors.affiliation: Department of Urology; Centre Hospitalier Universitaire Vaudois and University of Lausanne; Lausanne, Switzerland.
authors.forename: Loane
authors.initials: L
authors.lastname: Decrausaz
authors.forename: Christelle
authors.initials: C
authors.lastname: Pythoud
authors.forename: Sonia
authors.initials: S
authors.lastname: Domingos-Pereira
authors.forename: Laurent
authors.initials: L
authors.lastname: Derré
authors.forename: Patrice
authors.initials: P
authors.lastname: Jichlinski
authors.forename: Denise
authors.initials: D
authors.lastname: Nardelli-Haefliger
keywords: CD8+ T cells; bacterial immunostimulant; genital mucosa; human papillomavirus; vaccination