title: Study on performance of magnetic fluorescent nanoparticles as gene carrier and location in pig kidney cells.
abstract: We evaluated the performance of green fluorescent magnetic Fe3O4 nanoparticles (NPs) as gene carrier and location in pig kidney cells. When the mass ratio of NPs to green fluorescent protein plasmid DNA reached 1:16 or above, DNA molecules can be combined completely with NPs, which indicates that the NPs have good ability to bind negative DNA. Atomic force microscopy (AFM) experiments were carried out to investigate the binding mechanism between NPs and DNA. AFM images show that individual DNA strands come off of larger pieces of netlike agglomerations and several spherical nanoparticles are attached to each individual DNA strand and interact with each other. The pig kidney cells were labelled with membrane-specific red fluorescent dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate and nucleus-specific blue fluorescent dye 4',6-diamidino-2-phenylindole dihydrochloride. We found that green fluorescent nanoparticles can past the cell membrane and spread throughout the interior of the cell. The NPs seem to locate more frequently in the cytoplasm than in the nucleus.
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. haixincui@ieda.org.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Haixin
authors.initials: H
authors.lastname: Cui
authors.forename: Changjiao
authors.initials: C
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: Jinhui
authors.initials: J
authors.lastname: Cui
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao