title: Effective reduction of Vibrio vulnificus in the Eastern oyster (Crassostrea virginica) using high salinity depuration.
abstract: Depuration under different salinities was used to reduce the human pathogen Vibrio vulnificus from Eastern oysters (Crassostrea virginica). Individual recirculating systems were used to test the efficacy of depuration at three salinities (15, 25, and 35 psu) in four independent trials during a 14 day period. Initial loads of V. vulnificus were higher than 10,000 MPN/g of oyster meat in all trials. Data showed that 25 and 35 psu treatments were more efficient in reducing V. vulnificus numbers than 15 psu with an overall reduction of >3 logs. A significant decrease in MPN/g was observed as early as day 6 and further reductions were observed at day 10, while longer depurations did not improve efficacy. Only the highest salinity (35 psu) was capable of reducing V. vulnificus numbers to the FDA recommended level of <30 MPN/g in two of the four trials. Oysters survived well in the depuration systems with minimal mortality (<1%) but their condition index (meat quality and yield) decreased during the 14 day period in all treatments. The data presented in this study suggests that high salinity depuration is a promising method to reduce V. vulnificus in oysters.
authors.affiliation: Aquatic Microbiology Laboratory, Department of Fisheries and Allied Aquacultures, Auburn University, 203 Swingle Hall, Auburn, AL 36849, USA.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Larsen
authors.forename: F Scott
authors.initials: FS
authors.lastname: Rikard
authors.forename: William C
authors.initials: WC
authors.lastname: Walton
authors.forename: Covadonga R
authors.initials: CR
authors.lastname: Arias
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000187:drug effects / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D012758:Shellfish / Q000382:microbiology*; D012965:Sodium Chloride / Q000494:pharmacology*; D041261:Vibrio vulnificus / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D012965:Sodium Chloride