title: An ultrasensitive chemiluminescence turn-on assay for protease and inhibitor screening with a natural substrate.
abstract: A highly sensitive and convenient chemiluminescence (CL) turn-on assay for protease without any label and synthesis has been developed. Cytochrome c was digested by a protease, and heme was released as a peptide-heme conjugate, which greatly enhanced the luminol-H2O2 CL reaction.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D057075:Enzyme Assays; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008958:Models, Molecular; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism*; D011480:Protease Inhibitors / Q000032:analysis* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D001688:Biological Products; D011480:Protease Inhibitors; D006418:Heme; D008165:Luminol; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D010447:Peptide Hydrolases