title: Preparation of progenin III from total steroidal saponins of Dioscorea nipponica Makino using a crude enzyme from Aspergillus oryzae strain.
abstract: Progenin III, one of the most active spirostanol saponins, is a potential candidate for anti-cancer therapy due to its strong antitumor activity and low hemolytic activity. However, the concentration of progenin III is extremely low in natural Dioscorea plants. In this paper, the progenin III production from total steroidal saponins of Dioscorea nipponica Makino was studied using the crude enzyme from Aspergillus oryzae DLFCC-38. The crude enzyme converting total steroidal saponins into progenin III was obtained from the A. oryzae DLFCC-38 culture. For enzyme production, the strain was cultured for 72 h at 30 °C with shaking at 150 rpm in 5 % (w/v) malt extract medium containing 2 % (v/v) extract of D. nipponica as the enzyme inducer. The crude enzyme converted total steroidal saponins into major progenin III with a high yield when the reaction was carried out for 9 h at 50 °C and pH 5.0 with the 20 mg/ml of substrate. In the preparation of progenin III, 117 g of crude progenin III was obtained from 160 g of substrate, and the crude product was purified with silica gel column to obtain 60.3 g progenin III of 93.4 % purity.
authors.affiliation: College of Science, Yanbian University, Yanji 133002, People's Republic of China.
authors.forename: Tingqiang
authors.initials: T
authors.lastname: Liu
authors.forename: Hongshan
authors.initials: H
authors.lastname: Yu
authors.forename: Chunying
authors.initials: C
authors.lastname: Liu
authors.forename: Yongming
authors.initials: Y
authors.lastname: Bao
authors.forename: Xiangchun
authors.initials: X
authors.lastname: Hu
authors.forename: Yuanhao
authors.initials: Y
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Fu
authors.forename: Sihui
authors.initials: S
authors.lastname: Tang
authors.forename: Fengxie
authors.initials: F
authors.lastname: Jin
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D001709:Biotechnology; D001711:Biotransformation; D027742:Dioscorea / Q000737:chemistry*; D004798:Enzymes / Q000302:isolation & purification / Q000378:metabolism; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D004798:Enzymes; D012503:Saponins