title: Secondary T cell-T cell synaptic interactions drive the differentiation of protective CD8+ T cells.
abstract: Immunization results in the differentiation of CD8+ T cells, such that they acquire effector abilities and convert into a memory pool. Priming of T cells takes place via an immunological synapse formed with an antigen-presenting cell (APC). By disrupting synaptic stability at different times, we found that the differentiation of CD8+ T cells required cell interactions beyond those made with APCs. We identified a critical differentiation period that required interactions between primed T cells. We found that T cell-T cell synapses had a major role in the generation of protective CD8+ T cell memory. T cell-T cell synapses allowed T cells to polarize critical secretion of interferon-γ (IFN-γ) toward each other. Collective activation and homotypic clustering drove cytokine sharing and acted as regulatory stimuli for T cell differentiation.
authors.affiliation: Department of Pathology, University of California, San Francisco, San Francisco, California, USA. audrey.gerard@ucsf.edu
authors.forename: Audrey
authors.initials: A
authors.lastname: Gérard
authors.forename: Omar
authors.initials: O
authors.lastname: Khan
authors.forename: Peter
authors.initials: P
authors.lastname: Beemiller
authors.forename: Erin
authors.initials: E
authors.lastname: Oswald
authors.forename: Joyce
authors.initials: J
authors.lastname: Hu
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Matloubian
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Krummel
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology*; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D007156:Immunologic Memory; D054992:Immunological Synapses; D051379:Mice; D018345:Mice, Knockout; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines