title: Comparison of mitotic index and Ki67 index in the prognostication of canine cutaneous mast cell tumours.
abstract: Proliferation markers are commonly used for prognostication of mast cell tumours. The aim of the study is to compare the relative abilities of Ki67 and mitotic index to predict survival in the same cohort of dogs with cutaneous MCTs. Histological grade, mitotic index and Ki67 index were performed in all samples and clinical information was obtained by a follow-up questionnaire. Ninety-five dogs were included in the study with a median follow-up of 1145 days. Survival times varied significantly between categories of histological grade, mitotic index and Ki67 index. Multivariable analyses showed that the risk of dying due to MCT was similar in dogs with increased Ki67 index [hazard ratio, HR: 3.0 (95% CI 1.3-6.8)] or increased mitotic index [HR: 2.7 (95% CI 1.1-6.5)]. In conclusion, both mitotic index and Ki67 index were able to independently differentiate MCTs with worse prognosis. This distinction is particularly meaningful in selecting intermediate grade MCTs that may benefit from more aggressive local or systemic treatment.
authors.affiliation: Centre for Small Animal Studies, Animal Heath Trust, Newmarket, UK.
authors.forename: D
authors.initials: D
authors.lastname: Berlato
authors.affiliation: Centre for Small Animal Studies, Animal Heath Trust, Newmarket, UK.
authors.forename: S
authors.initials: S
authors.lastname: Murphy
authors.affiliation: Central Diagnostic Services, The Queen's Veterinary School Hospital, University of Cambridge, Cambridge, UK.
authors.forename: P
authors.initials: P
authors.lastname: Monti
authors.affiliation: Diagnostic Laboratory, Animal Heath Trust, Newmarket, UK.
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.affiliation: Equine Epidemiology and Disease Surveillance Unit, Animal Heath Trust, Newmarket, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Newton
authors.affiliation: Diagnostic Laboratory, Animal Heath Trust, Newmarket, UK.
authors.forename: A
authors.initials: A
authors.lastname: Flindall
authors.affiliation: Division of Virology, National Institute for Medical Research, London, UK.
authors.forename: G A
authors.initials: GA
authors.lastname: Maglennon
mesh_terms: D000818:Animals; D015415:Biomarkers; D015331:Cohort Studies; D004283:Dog Diseases / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D004285:Dogs; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D034801:Mastocytoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D008940:Mitotic Index; D009367:Neoplasm Staging / Q000662:veterinary*; D011379:Prognosis; D012878:Skin Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D016019:Survival Analysis; D013997:Time Factors
chemical_list: D015415:Biomarkers; D019394:Ki-67 Antigen
keywords: Ki67 index; mast cell tumour; mitotic index; oncology; pathology; prognosis; proliferative markers