title: Anti-Helicobacter pylori compounds from the ethanol extracts of Geranium wilfordii.
abstract: ETHNOPHARMOCOLOGICAL RELEVANCE: Geranium wilfordii Maxim has been extensively used in Chinese Herbal Medicine for treating gastrointestinal disorders, diarrhea and dysentery. In the current study we aimed to investigate the anti-Helicobacter pylori activity of ethanol extracts of Geranium wilfordii Maxim and its main active compounds, corilagin and 1,2,3,6-tetra-O-galloyl-β-D-glucose.
  
  
  METHODS
  The plant materials were extracted three times with ethanol and the concentrated filtrate was successively fractioned into chloroform, ethyl acetate, and n-BuOH-soluble portions which were examined in vitro for the anti-Helicobacter. pylori activity. Employing a standard strain and five clinical isolates of Helicobacter pylori, the extract, fractions and compounds of Geranium wilfordii Maxim were assessed in vitro.
  
  
  RESULTS
  The ethanol fraction, ethyl acetate fraction, corilagin, and 1,2,3,6-tetra-O-galloyl-β-D-glucose were found to be strongly inhibitory to Helicobacter. pylori (MICs: 40, 30, 4, and 8μg/ml respectively).
  
  
  CONCLUSIONS
  The results of the present study showed that the ethanol and the ethyl acetate extracts from Geranium wilfordii Maxim displayed as well the most significant inhibition to the growth of Helicobacter. pylori, of which corilagin and 1,2,3,6-tetra-O-galloyl-β-D-glucose have been identified main anti-Helicobacter pylori active constituents.
authors.affiliation: Jiangsu Chia-Tai Tianqing Pharmaceutical Research Institute, Nanjing 210042, PR China.
authors.forename: Xi-Quan
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Gu
authors.forename: Xin-Zhu
authors.initials: XZ
authors.lastname: Li
authors.forename: Zhong-Nan
authors.initials: ZN
authors.lastname: Xu
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D005591:Chemical Fractionation; D002725:Chloroform / Q000737:chemistry; D052978:Disk Diffusion Antimicrobial Tests; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000431:Ethanol / Q000737:chemistry*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D031315:Geranium / Q000737:chemistry; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development; D047348:Hydrolyzable Tannins; D007928:Lethal Dose 50; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry*
chemical_list: D000085:Acetates; D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D047348:Hydrolyzable Tannins; D012997:Solvents; D000431:Ethanol; C049096:corilagin; D005707:Gallic Acid; C007650:ethyl acetate; C060475:1,2,3,6-tetragalloylglucose; D002725:Chloroform; D020001:1-Butanol; D005947:Glucose