title: Molecular sentinel-on-chip for SERS-based biosensing.
abstract: The development of DNA detection techniques on large-area plasmonics-active platforms is critical for many medical applications such as high-throughput screening, medical diagnosis and systems biology research. Here, we report for the first time a unique "molecular sentinel-on-chip" (MSC) technology for surface-enhanced Raman scattering (SERS)-based DNA detection. This unique approach allows label-free detection of DNA molecules on chips developed on a wafer scale using large area nanofabrication methodologies. To develop plasmonics-active biosensing platforms in a repeatable and reproducible manner, we employed a combination of deep UV lithography, atomic layer deposition, and metal deposition to fabricate triangular-shaped nanowire (TSNW) arrays having controlled sub-10 nm gap nanostructures over an entire 6 inch wafer. The detection of a DNA sequence of the Ki-67 gene, a critical breast cancer biomarker, on the TSNW substrate illustrates the usefulness and potential of the MSC technology as a novel SERS-based DNA detection method.
authors.affiliation: Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA.|Department of Biomedical Engineering, Duke University, Durham, NC, USA.
authors.forename: Hsin-Neng
authors.initials: HN
authors.lastname: Wang
authors.affiliation: Department of Electrical Engineering, Indian Institute of Technology Delhi, New Delhi, India.
authors.forename: Anuj
authors.initials: A
authors.lastname: Dhawan
authors.affiliation: Department of Electrical & Computer Engineering, NC State University, Raleigh, NC, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Materials Science and Engineering, NC State University, Raleigh, NC, USA.
authors.forename: Dale
authors.initials: D
authors.lastname: Batchelor
authors.affiliation: Department of Physics and Astronomy, Appalachian State University, Boone, NC, USA.
authors.forename: Donovan N
authors.initials: DN
authors.lastname: Leonard
authors.affiliation: Department of Electrical & Computer Engineering, NC State University, Raleigh, NC, USA.
authors.forename: Veena
authors.initials: V
authors.lastname: Misra
authors.affiliation: Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA.|Department of Biomedical Engineering, Duke University, Durham, NC, USA.|Department of Chemistry, Duke University, Durham, NC, USA.
authors.forename: Tuan
authors.initials: T
authors.lastname: Vo-Dinh
mesh_terms: D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics; D053770:Nanowires / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000378:metabolism; D012825:Silicon / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D015415:Biomarkers; D019394:Ki-67 Antigen; D015345:Oligonucleotide Probes; D004247:DNA; D012825:Silicon