title: Acetylcholinesterase-inhibitory activities of the extracts from sponges collected in mauritius waters.
abstract: Patients diagnosed with Alzheimer's disease (AD) show a characteristic neurochemical deficit of acetylcholine, especially in the basal forebrains. The use of acetylcholinesterase (AChE) inhibitors to retard the hydrolysis of acetylcholine has been suggested as a promising strategy for AD treatment. In this study, we evaluated the acetylcholinesterase inhibitory (AChEI) activities of 134 extracts obtained from 45 species of marine sponges. Thin-layer chromatography (TLC) and microplate assays reveal potent acetylcholinsterase inhibitory activities of two AcOEt extracts from the sponges Pericharax heteroraphis and Amphimedon navalis PULITZER-FINALI. We further investigated the inhibitory kinetics of the extracts and found them to display mixed competitive/noncompetitive inhibition and associated their inhibitory activity partly to terpenoids. Acetylcholinesterase inhibitors from marine organisms have been rarely studied, and this study demonstrated the potential of marine sponges as a source of pharmaceutical leads against neurodegenerative diseases.
authors.affiliation: Mauritius Oceanography Institute, Quatre-Bornes, Mauritius.
authors.forename: Girish
authors.initials: G
authors.lastname: Beedessee
authors.forename: Avin
authors.initials: A
authors.lastname: Ramanjooloo
authors.forename: Rashmee
authors.initials: R
authors.lastname: Surnam-Boodhun
authors.forename: Rob W M
authors.initials: RW
authors.lastname: van Soest
authors.forename: Daniel E P
authors.initials: DE
authors.lastname: Marie
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D000544:Alzheimer Disease / Q000188:drug therapy; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D002855:Chromatography, Thin Layer; D006801:Humans; D007700:Kinetics; D008436:Mauritius; D011161:Porifera / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase