title: Role of regulated upon activation normal T-cell expressed and secreted in a model of retransplantation acute rejection mediated by alloreactive memory CD4+ T cells.
abstract: BACKGROUND
  It is unknown what role Regulated Upon Activation Normal T-Cell Exposed and Secreted may play in retransplantation or T-cell memory-transfer models. The experiment observed the influence of the chemokine RANTES in a mouse model of acute cardiac allograft rejection induced by adoptive transfer of alloreactive CD4(+) memory T (Tm) cells.
  
  
  METHODS
  Alloreactive CD4(+) Tm cells from spleens of skin-grafted C57BL/6 were adoptively transferred to naïve C57BL/6 recipients prior to heterotopic heart transplantation. We measured the median survival time of cardiac grafts and performed some tests.
  
  
  RESULTS
  Spleens from skin-grafted C57BL/6 contained 26.83% CD4(+) Tm cells. The median graft survival time of heterotopic heart transplantations (n = 6) was 5.17 ± 0.17 days for hosts receiving CD4(+) Tm cells compared with 7.76 ± 0.21 days among controls (n = 6; P < .001). The mean rejection activity in histological sections of cardiac allografts at day 5 postgrafting was 3.92 ± 0.08 in the CD4(+) Tm cell recipient group (n = 6) compared with 2.67 ± 0.14 in the controls (n = 6; P < .001). Gene expression of Ccl5, interferon (IFN)-γ and interleukin2 was significantly higher among CD4(+) Tm recipients compared with controls. Serum concentrations of RANTES and IFN-γ were higher in the heterotopic heart transplantation group receiving CD4(+) Tm compared with controls.
  
  
  CONCLUSIONS
  Alloreactive CD4(+) Tm cells contribute to increased expression and secretion of RANTES, and to the Tm and other inflammatory cells migration into the graft.
authors.affiliation: Department of Cardiac Surgery, The First Affiliated Hospital, Xiamen University, Fujian Province, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Shan
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.forename: Z
authors.initials: Z
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Qiu
authors.forename: F
authors.initials: F
authors.lastname: Kuang
authors.forename: J
authors.initials: J
authors.lastname: Zhuang
authors.forename: Z
authors.initials: Z
authors.lastname: Qi
mesh_terms: D000208:Acute Disease; D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D002465:Cell Movement; D018946:Chemokine CCL5 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D006084:Graft Rejection / Q000097:blood / Q000276:immunology* / Q000473:pathology; D006085:Graft Survival; D016027:Heart Transplantation / Q000009:adverse effects / Q000276:immunology*; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D016038:Skin Transplantation / Q000276:immunology; D013997:Time Factors
chemical_list: C503460:Ccl5 protein, mouse; D018946:Chemokine CCL5; D007376:Interleukin-2; D007371:Interferon-gamma