title: μHall chip for sensitive detection of bacteria.
abstract: Sensitive, rapid and phenotype-specific enumeration of pathogens is essential for the diagnosis of infectious disease, monitoring of food chains, and for defense against bioterrorism. Microbiological culture and genotyping, techniques that sensitively and selectively detect bacteria in laboratory settings, have limited application in clinical environments due to high cost, slow response times, and the need for specially trained staff and laboratory infrastructure. To address these challenges, we developed a microfluidic chip-based micro-Hall (μHall) platform capable of measuring single, magnetically tagged bacteria directly in clinical specimens with minimal sample processing. We demonstrated the clinical utility of the μHall chip by enumerating Gram-positive bacteria. The overall detection limit of the system was similar to that of culture tests (~10 bacteria), but the assay time was 50-times faster. This low-cost, single-cell analytical technique is especially well-suited to diagnose infectious diseases in resource-limited clinical settings.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114.
authors.forename: David
authors.initials: D
authors.lastname: Issadore
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Chung
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Chung
authors.forename: Ghyslain
authors.initials: G
authors.lastname: Budin
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D003141:Communicable Diseases / Q000175:diagnosis / Q000382:microbiology; D034242:Cyclooctanes / Q000737:chemistry; D005838:Genotype; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D034242:Cyclooctanes; D058185:Magnetite Nanoparticles; D014640:Vancomycin
keywords: bacterial detection; magnetic nanoparticles; μHall