title: Microbial transformations of isophorone by Alternaria alternata and Neurospora crassa.
abstract: Isophorone (3,5,5-trimethyl-2-cyclohexen-1-one), a monoterpene, and the structurally related 1,8-cineole and camphor, have demonstrated a protective effect against cancer, biological activity against a variety of microorganisms, and anti-oxidant properties. The derivatization of isophorone is, therefore, an important field of xenobiochemistry, pharmacology and toxicology. The aim of this study was to obtain derivatives of isophorone through microbial biotransformation and evaluate the biotransformation metabolites as potential antimicrobial agents. Incubation of isophorone with the fungi Alternaria alternata and Neurospora crassa afforded 4a-hydroxy- and 7-hydroxy-isophorone as transformation metabolites. The antimicrobial activities of isophorone and the metabolites were evaluated in vitro both by using agar dilution and microdilution methods. However, no significant antibacterial activity was observed when compared with those of standard substances.
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Eskisehir Osmangazi University, 26480, Eskişehir, Turkey. ikiran@ogu.edu.tr
authors.forename: Ismail
authors.initials: I
authors.lastname: Kiran
authors.forename: Ozge
authors.initials: O
authors.lastname: Ozşen
authors.forename: Turgay
authors.initials: T
authors.lastname: Celik
authors.forename: Semra
authors.initials: S
authors.lastname: Ilhan
authors.forename: Bükay Yenice
authors.initials: BY
authors.lastname: Gürsu
authors.forename: Fatih
authors.initials: F
authors.lastname: Demirci
mesh_terms: D000528:Alternaria / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000032:analysis*; D001711:Biotransformation; D003512:Cyclohexanones / Q000737:chemistry / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009492:Neurospora crassa / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D003512:Cyclohexanones; C005940:isophorone