title: Comparison of the efficiency of Banna miniature inbred pig somatic cell nuclear transfer among different donor cells.
abstract: Somatic cell nuclear transfer (SCNT) is an important method of breeding quality varieties, expanding groups, and preserving endangered species. However, the viability of SCNT embryos is poor, and the cloned rate of animal production is low in pig. This study aims to investigate the gene function and establish a disease model of Banna miniature inbred pig. SCNT with donor cells derived from fetal, newborn, and adult fibroblasts was performed, and the cloning efficiencies among the donor cells were compared. The results showed that the cleavage and blastocyst formation rates did not significantly differ between the reconstructed embryos derived from the fetal (74.3% and 27.4%) and newborn (76.4% and 21.8%) fibroblasts of the Banna miniature inbred pig (P>0.05). However, both fetal and newborn fibroblast groups showed significantly higher rates than the adult fibroblast group (61.9% and 13.0%; P<0.05). The pregnancy rates of the recipients in the fetal and newborn fibroblast groups (60% and 80%, respectively) were higher than those in the adult fibroblast group. Eight, three, and one cloned piglet were obtained from reconstructed embryos of the fetal, newborn, and adult fibroblasts, respectively. Microsatellite analyses results indicated that the genotypes of all cloning piglets were identical to their donor cells and that the genetic homozygosity of the Banna miniature inbred pig was higher than those of the recipients. Therefore, the offspring was successfully cloned using the fetal, newborn, and adult fibroblasts of Banna miniature inbred pig as donor cells.
authors.affiliation: Key Laboratory of Banna Miniature Inbred Pig of Yunnan Province, Yunnan Agricultural University, Kunming, China. hongjiangwei@126.com
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Wei
authors.forename: Yubo
authors.initials: Y
authors.lastname: Qing
authors.forename: Weirong
authors.initials: W
authors.lastname: Pan
authors.forename: Hongye
authors.initials: H
authors.lastname: Zhao
authors.forename: Honghui
authors.initials: H
authors.lastname: Li
authors.forename: Wenmin
authors.initials: W
authors.lastname: Cheng
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
authors.forename: Chengsheng
authors.initials: C
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Li
authors.forename: Guowen
authors.initials: G
authors.lastname: Fu
authors.forename: Wengui
authors.initials: W
authors.lastname: Li
authors.forename: Jige
authors.initials: J
authors.lastname: Xin
authors.forename: Yangzhi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019976:Cloning, Organism; D004247:DNA / Q000235:genetics; D010064:Embryo Implantation; D047108:Embryonic Development; D005260:Female; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D007178:Inbreeding; D008297:Male; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D013552:Swine; D013556:Swine, Miniature / Q000196:embryology / Q000235:genetics*
chemical_list: D004247:DNA