title: Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-d tooth culture.
abstract: We investigated the short-term effect of LIPUS on human dentin-pulp complex in vitro. We collected sixty-three premolars from patients who needed the extraction. The premolars were sectioned transversely into 600-μm-thick slices, and then divided into five groups according to LIPUS application time (control, 5, 10, 15 and 20 min). LIPUS transducer produced an incident intensity of 30 mW/cm(2). After 24 h, tissue was harvested for histomorphometrical analysis and RT-PCR (Genes of interest: Collagen I, DMP1, DSPP, TGF β1, RANKL and OPG). Histomorphometric analysis showed no significant difference among the five groups in the odontoblast count and predentin thickness. RT-PCR demonstrated no expression of TGF β1, low amounts of DSPP, a twofold increase in collagen I expression in the 5- and 10-minute LIPUS groups and a threefold increase in DMP1 expression in the 10-minute LIPUS group. LIPUS application was stimulatory to the dentin-pulp complex in vitro and increased the expression of collagen I and DMP1.
authors.affiliation: University of Alberta, Department of Dentistry, Faculty of Medicine & Dentistry, Orthodontic Graduate Program, Edmonton, AB, Canada. aldaghre@ualberta.ca
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Daghreer
authors.forename: Michael
authors.initials: M
authors.lastname: Doschak
authors.forename: Alastair J
authors.initials: AJ
authors.lastname: Sloan
authors.forename: Paul W
authors.initials: PW
authors.lastname: Major
authors.forename: Giseon
authors.initials: G
authors.lastname: Heo
authors.forename: Cristian
authors.initials: C
authors.lastname: Scurtescu
authors.forename: Ying Y
authors.initials: YY
authors.lastname: Tsui
authors.forename: Tarek
authors.initials: T
authors.lastname: El-Bialy
mesh_terms: D001641:Bicuspid / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D002648:Child; D024042:Collagen Type I / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D003804:Dentin / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D019220:High-Energy Shock Waves; D006801:Humans; D008297:Male; D009924:Organ Culture Techniques; D010750:Phosphoproteins / Q000378:metabolism; D011829:Radiation Dosage; D014464:Ultrasonic Therapy / Q000379:methods*
chemical_list: D024042:Collagen Type I; C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins