title: Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches.
abstract: Metabolomics provides an invaluable means to interrogate the function of "orphan" enzymes, i.e., those whose endogenous substrates are not known. Here we describe a high-performance liquid chromatography-coupled mass spectrometry (HPLC-MS)-based metabolomics approach to identify an endogenous substrate of an orphan cytochrome P450.
authors.affiliation: Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Cheng
authors.forename: F Peter
authors.initials: FP
authors.lastname: Guengerich
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D011485:Protein Binding; D012984:Software
chemical_list: D003577:Cytochrome P-450 Enzyme System