title: The 5' untranslated region of the serotonin receptor 2C pre-mRNA generates miRNAs and is expressed in non-neuronal cells.
abstract: The serotonin receptor 2C (HTR2C) gene encodes a G protein-coupled receptor that is exclusively expressed in neurons. Here, we report that the 5' untranslated region of the receptor pre-mRNA as well as its hosted miRNAs is widely expressed in non-neuronal cell lines. Alternative splicing of HTR2C is regulated by MBII-52. MBII-52 and the neighboring MBII-85 cluster are absent in people with Prader-Willi syndrome, which likely causes the disease. We show that MBII-52 and MBII-85 increase expression of the HTR2C 5' UTR and influence expression of the hosted miRNAs. The data indicate that the transcriptional unit expressing HTR2C is more complex than previously recognized and likely deregulated in Prader-Willi syndrome.
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY, 40536, USA.
authors.forename: Zhaiyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Marina
authors.initials: M
authors.lastname: Falaleeva
authors.forename: Lily
authors.initials: L
authors.lastname: Agranat-Tamir
authors.forename: Amadis
authors.initials: A
authors.lastname: Pages
authors.forename: Eduardo
authors.initials: E
authors.lastname: Eyras
authors.forename: Joseph
authors.initials: J
authors.lastname: Sperling
authors.forename: Ruth
authors.initials: R
authors.lastname: Sperling
authors.forename: Stefan
authors.initials: S
authors.lastname: Stamm
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011218:Prader-Willi Syndrome / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D035683:MicroRNAs; D012322:RNA Precursors; D044405:Receptor, Serotonin, 5-HT2C; D012701:Serotonin