title: ATM/ATR-dependent Tyr15 phosphorylation of cyclin-dependent kinases in response to hydroxyurea in Vicia faba root meristem cells.
abstract: DNA damage or stalled replication forks activate cell cycle checkpoints. However, the regulation of metabolic pathways that are responsible for maintenance of genome integrity in plants is still largely unknown. Present research on Vicia faba root meristem cells indicates that inhibitory phosphorylation of cyclin-dependent kinases (Cdks) at Tyr15 plays a prominent role during blockage of cell cycle in response to genotoxic stress. Phosphorylation of P-loop in Cdks takes place in ATM/ATR-dependent manner. Although, Tyr15 phosphorylation upon hydroxyurea (HU) treatment was found in most cells classified to G1 and S phase, interestingly, the number of phoshpo-Tyr15-positive cells decreases in G2 phase. Presented data confirm much similarity in regulation of Cdks functions under genotoxic stress between plants and animals; however, they may also substantiate evolutionarily developed differences especially in regulation of G2/M transition between these two kingdoms.
authors.affiliation: Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Lodz, ul. Pomorska 141/143, 90-236, Lodz, Poland, konrad.s.krajewski@gmail.com.
authors.forename: Konrad
authors.initials: K
authors.lastname: Krajewski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D016193:G1 Phase / Q000187:drug effects / Q000502:physiology; D006918:Hydroxyurea / Q000494:pharmacology*; D018519:Meristem / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D016196:S Phase / Q000187:drug effects / Q000502:physiology; D014443:Tyrosine / Q000378:metabolism; D031307:Vicia faba / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014443:Tyrosine; D064007:Ataxia Telangiectasia Mutated Proteins; D018844:Cyclin-Dependent Kinases; D046988:Proteasome Endopeptidase Complex; D006918:Hydroxyurea