title: Scanning electron microscopy evaluation of endothelialized tissue-engineered constructs.
abstract: Scanning electron microscopy (SEM) is an important technique for evaluation of the efficiency of endothelialization of tissue-engineered constructs incorporating a surface endothelial cell layer. Here, we describe methodologies for the preparation of such constructs for SEM analysis that are applicable to a broad range of tissue-engineered constructs.
authors.affiliation: Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA.
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Johnson
mesh_terms: D000818:Animals; D004727:Endothelium / Q000166:cytology / Q000648:ultrastructure*; D005976:Glutaral; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009993:Osmium Tetroxide; D023822:Tissue Engineering / Q000379:methods*; D058017:Vascular Grafting / Q000379:methods
chemical_list: D009993:Osmium Tetroxide; D005976:Glutaral