title: Glucosamine-conjugated nanoparticles for the separation of insulin-secreting beta cells.
abstract: A small molecule, glucosamine, is used as targeting moiety for insulin-secreting beta cell separation in artificial cell mixtures and tissue samples. The specificity of glucosamine allows it to be used in cell sorting applications. In addition, a thrombin-specific cleavable peptide was used as an intermediary to release nanoparticles from cell surfaces to facilitate cell attachment and proliferation.
authors.affiliation: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669 Singapore.
authors.forename: Karthikeyan
authors.initials: K
authors.lastname: Narayanan
authors.forename: Alex W H
authors.initials: AW
authors.lastname: Lin
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Nandanan
authors.initials: N
authors.lastname: Erathodiyil
authors.forename: Andrew C A
authors.initials: AC
authors.lastname: Wan
authors.forename: Jackie Y
authors.initials: JY
authors.lastname: Ying
mesh_terms: D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry; D005944:Glucosamine / Q000737:chemistry* / Q000378:metabolism; D051273:Glucose Transporter Type 2 / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D045663:Quantum Dots / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D051273:Glucose Transporter Type 2; D010455:Peptides; D005944:Glucosamine
keywords: beta cell separation; glucosamine; iron oxide nanoparticles; quantum dots