title: Fabrication of transplantable corneal epithelial and oral mucosal epithelial cell sheets using a novel temperature-responsive closed culture device.
abstract: Temperature-responsive culture surfaces make it possible to harvest transplantable carrier-free cell sheets. Here, we applied temperature-responsive polymer for polycarbonate surfaces with previously developed closed culture devices for an automated culture system in order to fabricate transplantable stratified epithelial cell sheets. Histological and immunohistochemical analyses and colony-forming assays revealed that corneal epithelial and oral mucosal epithelial cell sheets could be harvested with the temperature-responsive closed culture devices. The results were similar to those obtained using temperature-responsive culture inserts. These results indicate that the novel temperature-responsive closed culture device is useful for fabricating transplantable stratified epithelial cell sheets.
authors.affiliation: Central Research Laboratory, Hitachi Ltd, Saitama, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Nakajima
authors.forename: Toyoshige
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Kitano
authors.forename: Hiroya
authors.initials: H
authors.lastname: Watanabe
authors.forename: Manabu
authors.initials: M
authors.lastname: Mizutani
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nozaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Senda
authors.forename: Kazuo
authors.initials: K
authors.lastname: Saitoh
authors.forename: Ryo
authors.initials: R
authors.lastname: Takagi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
authors.forename: Shizu
authors.initials: S
authors.lastname: Takeda
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001331:Automation; D018929:Cell Culture Techniques / Q000379:methods; D003315:Cornea / Q000473:pathology*; D003470:Culture Media; D004847:Epithelial Cells / Q000166:cytology*; D007150:Immunohistochemistry; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009061:Mouth Mucosa / Q000473:pathology*; D011076:Polycarboxylate Cement / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011817:Rabbits; D013234:Stem Cells; D013696:Temperature; D023822:Tissue Engineering
chemical_list: D003470:Culture Media; D011076:Polycarboxylate Cement; D011108:Polymers; C005506:polycarbonate
keywords: automated culture system; cell sheet; closed cell culture; culture device; epithelial cell; temperature-responsive culture surface