title: Transcriptome analysis of Pacific white shrimp (Litopenaeus vannamei) hepatopancreas in response to Taura syndrome Virus (TSV) experimental infection.
abstract: BACKGROUND
  The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology.
  
  
  RESULTS
  We obtained 126919 and 102181 high-quality reads from TSV-infected and non-infected (control) L. vannamei cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 15004 unigenes, with an average length of 507 bp. Based on BLASTX search (E-value <10-5) against NR, Swissprot, GO, COG and KEGG databases, 10425 unigenes (69.50% of all unigenes) were annotated with gene descriptions, gene ontology terms, or metabolic pathways. In addition, we identified 770 microsatellites and designed 497 sets of primers. Comparative genomic analysis revealed that 1311 genes differentially expressed in the infected shrimp compared to the controls, including 559 up- and 752 down- regulated genes. Among the differentially expressed genes, several are involved in various animal immune functions, such as antiviral, antimicrobial, proteases, protease inhibitors, signal transduction, transcriptional control, cell death and cell adhesion.
  
  
  CONCLUSIONS
  This study provides valuable information on shrimp gene activities against TSV infection. Results can contribute to the in-depth study of candidate genes in shrimp immunity, and improves our current understanding of this host-virus interaction. In addition, the large amount of transcripts reported in this study provide a rich source for identification of novel genes in shrimp.
authors.affiliation: Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Institute of Fisheries, Nanning, China.
authors.forename: Digang
authors.initials: D
authors.lastname: Zeng
authors.forename: Xiuli
authors.initials: X
authors.lastname: Chen
authors.forename: Daxiang
authors.initials: D
authors.lastname: Xie
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Zhao
authors.forename: Chunling
authors.initials: C
authors.lastname: Yang
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Liao
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D003445:Crustacea / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D056629:Dicistroviridae / Q000235:genetics*; D043143:Hepatopancreas / Q000378:metabolism* / Q000503:physiopathology; D018895:Microsatellite Repeats / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary