title: Forensic validation of the modified ABO genotyping method using a DNA chip.
abstract: ABO typing is effective in several forensic investigations, including the identification of unknown cadavers. When the serological method cannot be used because of the decomposition of ABH antigens, ABO genotyping of DNA is often performed. Previously, we reported a novel ABO genotyping method using a DNA chip as a proof of concept. This chip can simultaneously detect single nucleotide polymorphisms in the ABO gene and a part of the primate-specific D17Z1 sequence for human identification. In the present study, this method was modified and further validated for forensic use. We demonstrated that the modified method can correctly perform ABO genotyping and human identification if the appropriate amount of template (>0.5ng of DNA) is analyzed. Moreover, it was found that this chip method can be used to type highly degraded DNA. This method is expected to be a useful supplemental tool for the identification of individuals from highly decomposed samples.
authors.affiliation: National Research Institute of Police Science, Chiba 277-0822, Japan. k-watanabe@nrips.go.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Watanabe
authors.forename: Mayuko
authors.initials: M
authors.lastname: Hosoya
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirayama
authors.forename: Hisako
authors.initials: H
authors.lastname: Saitoh
authors.forename: Hirotaro
authors.initials: H
authors.lastname: Iwase
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akutsu
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Sakurada
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D053803:Forensic Genetics; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D000017:ABO Blood-Group System