title: Rapid and intermediate N-acetylators are less susceptible to oxidative damage among 4,4'-methylenebis(2-chloroaniline) (MBOCA)-exposed workers.
abstract: OBJECTIVE
  In this study, we explored the association between a marker of oxidative stress, 8-hydroxydeoxyguanosine (8-OHdG), and genetic polymorphism of the carcinogen-metabolizing enzyme N-acetyltransferase 2 (NAT2) among 4,4'-methylenebis(2-chloroaniline) (MBOCA)-exposed workers.
  
  
  METHODS
  The study population was recruited from four MBOCA-producing factories, and included 57 MBOCA-exposed workers and 101 unexposed control workers. Personal characteristics were collected by questionnaire. Plasma 8-OHdG levels were measured by LC/MS/MS. NAT2 alleles were measured by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  NAT2 polymorphism influenced the plasma 8-OHdG levels of MBOCA-exposed workers, but not of non-exposed workers. No difference between exposed and control groups was found for the crude 8-OHdG levels among rapid, intermediate, and slow acetylators. After adjusting for gender, age, smoking, and alcohol consumption habit, the 8-OHdG concentration in the MBOCA-exposed workers was 0.18pg/ml (95% CI -1.80 to -0.12) lower than the control group among rapid and intermediate acetylators. However, the difference between exposed and control groups was not significant for slow acetylators.
  
  
  CONCLUSIONS
  Gene-environment interactions could play a role in the carcinogenesis of occupational MBOCA exposure. We suggest that the impact of the NAT2 acetylator status is low, if at all, on the generation of the oxidative stress marker 8-OHdG in the investigated exposed group.
authors.affiliation: Department of Family Medicine & Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Neihu, Taipei, Taiwan, ROC.
authors.forename: I-Shen
authors.initials: IS
authors.lastname: Lin
authors.forename: Pao-Lou
authors.initials: PL
authors.lastname: Fan
authors.forename: Hong-I
authors.initials: HI
authors.lastname: Chen
authors.forename: Ching-Hui
authors.initials: CH
authors.lastname: Loh
authors.forename: Tung-Sheng
authors.initials: TS
authors.lastname: Shih
authors.forename: Saou-Hsing
authors.initials: SH
authors.lastname: Liou
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000107:Acetylation; D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D059647:Gene-Environment Interaction; D005838:Genotype; D006801:Humans; D008297:Male; D008753:Methylenebis(chloroaniline) / Q000378:metabolism*; D016273:Occupational Exposure / Q000032:analysis*; D018384:Oxidative Stress; D011110:Polymorphism, Genetic
chemical_list: D008753:Methylenebis(chloroaniline); D000080242:8-Hydroxy-2'-Deoxyguanosine; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human; D003849:Deoxyguanosine