title: Fabrication of graphene-platinum nanocomposite for the direct electrochemistry and electrocatalysis of myoglobin.
abstract: In this paper a platinum (Pt) nanoparticle decorated graphene (GR) nanosheet was synthesized and used for the investigation on direct electrochemistry of myoglobin (Mb). By integrating GR-Pt nanocomposite with Mb on the surface of carbon ionic liquid electrode (CILE), a new electrochemical biosensor was fabricated. UV-Vis absorption and FT-IR spectra indicated that Mb remained its native structure in the nanocomposite film. Electrochemical behaviors of Nafion/Mb-GR-Pt/CILE were investigated with a pair of well-defined redox peak appeared, which indicated that direct electron transfer of Mb was realized on the underlying electrode with the usage of the GR-Pt nanocomposite. The fabricated electrode showed good electrocatalytic activity to the reduction of trichloroacetic acid in the linear range from 0.9 to 9.0 mmol/L with the detection limit as 0.32 mmol/L (3σ), which showed potential application for fabricating novel electrochemical biosensors and bioelectronic devices.
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China. swyy26@hotmail.com
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Linfang
authors.initials: L
authors.lastname: Li
authors.forename: Bingxin
authors.initials: B
authors.lastname: Lei
authors.forename: Tongtong
authors.initials: T
authors.lastname: Li
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Ju
authors.forename: Xiuzheng
authors.initials: X
authors.lastname: Wang
authors.forename: Guangjiu
authors.initials: G
authors.lastname: Li
authors.forename: Zhenfan
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D002384:Catalysis; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005465:Fluorocarbon Polymers; D052578:Ionic Liquids; D009211:Myoglobin; C040402:perfluorosulfonic acid; D010984:Platinum; D006108:Graphite