title: Dihydrotestosterone and bicalutamide do not affect periostin expression in androgen-dependent LNCaP prostate cancer cell lines.
abstract: OBJECTIVE
  To investigate periostin (POSTN) expression in the LNCaP cell line.
  
  
  METHODS
  Our LNCaP strain did not constitutively express the POSTN gene. Through cell transfection with a cloning vector, we developed an LNCaP cell line that stably expressed POSTN. LNCaP wild-type and transfected cells were incubated with dihydrotestosterone (DHT) in the presence/or absence of bicalutamide (BIC). POSTN mRNA was detected by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and growth was measured with the MTT assay.
  
  
  RESULTS
  POSTN transfection stimulated LNCaP cell growth. While POSTN transfection did not interfere with the stimulatory effect of DHT, BIC had an inhibitory effect on cell proliferation. However, exposure to either DHT and/or BIC was not able to interfere with POSTN expression per se.
  
  
  CONCLUSIONS
  We confirmed the role of POSTN in promoting cancer cell growth. Although POSTN transcription is not likely to be androgen-dependent, the fact that increased cell proliferation POSTN-mediated was impaired by BIC suggests an androgen modulation of POSTN interaction proteins.
authors.affiliation: IRCCS San Martino University Hospital - IST National Cancer Research Institute and the University of Genoa, Academic Unit of Medical Oncology (UOC Oncologia Medica B), Largo Rosanna Benzi 10, 16132 Genoa, Italy. f.boccardo@unige.it
authors.forename: Francesca
authors.initials: F
authors.lastname: Argellati
authors.forename: Pier Vitale
authors.initials: PV
authors.lastname: Nuzzo
authors.forename: Francesco
authors.initials: F
authors.lastname: Ricci
authors.forename: Rosa
authors.initials: R
authors.lastname: Mangerini
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rubagotti
authors.forename: Francesco
authors.initials: F
authors.lastname: Boccardo
mesh_terms: D000728:Androgens / Q000502:physiology*; D000813:Anilides / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D013196:Dihydrotestosterone / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology*; D009570:Nitriles / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D014105:Tosyl Compounds / Q000494:pharmacology*
chemical_list: D000728:Androgens; D000813:Anilides; D015815:Cell Adhesion Molecules; D009570:Nitriles; C512835:POSTN protein, human; D012333:RNA, Messenger; D014105:Tosyl Compounds; D013196:Dihydrotestosterone; C053541:bicalutamide