title: Evaluation of salivary biomarker profiles following non-surgical management of chronic periodontitis.
abstract: OBJECTIVE
  Soluble toll-like receptor-2 (sTLR-2) and cytokines in saliva were assessed as clinical markers for chronic periodontitis in a longitudinal study.
  
  
  METHODS
  Unstimulated whole saliva was collected from 20 periodontally healthy individuals and 20 patients with chronic periodontitis at diagnosis and at 1 and 6 weeks following scaling and root planing (SRP). Biomarkers including the cytokines (IL-4, IL-6, IL-10, and IL-17), sTLR-2, and sCD14 in saliva were measured by enzyme-linked immunosorbent assay. Mann-Whitney U-test and Student's t-test were used to determine the significance between healthy and chronic periodontitis groups and that between pre- and post-SRP samples, respectively.
  
  
  RESULTS
  Salivary sTLR-2, IL-17, and IL-10 levels were significantly lower and those of sCD14, IL-6, and IL-4 were significantly higher in patients with chronic periodontitis as compared with healthy controls. Furthermore, sTLR-2 and IL-4 in saliva reached levels comparable to those of healthy individuals at 6-week re-evaluation visit, implicating a correlation of the two markers with the disease process.
  
  
  CONCLUSIONS
  Our data suggest that salivary sTLR-2 is a potential prognostic or maintenance marker for chronic periodontitis. The observed variability of salivary cytokines is consistent with the role of these cytokines in the progression of chronic periodontitis.
authors.affiliation: Department of Periodontics and Allied Dental Programs, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: S
authors.initials: S
authors.lastname: Prakasam
authors.forename: M
authors.initials: M
authors.lastname: Srinivasan
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D055113:Chronic Periodontitis / Q000175:diagnosis / Q000628:therapy*; D016207:Cytokines / Q000032:analysis*; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000737:chemistry*; D051195:Toll-Like Receptor 2 / Q000032:analysis*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D051195:Toll-Like Receptor 2
keywords: CD14 antigen; biological markers; chronic periodontitis; dental scaling; diagnosis; root planing; saliva; toll-like receptors