title: Preparation, drug release and cellular uptake of doxorubicin-loaded dextran-b-poly(ɛ-caprolactone) nanoparticles.
abstract: Amphiphilic dextran-b-poly(ɛ-caprolactone) diblock copolymers were synthesized with the purpose of preparing nanocarriers for doxorubicin (DOX), an anticancer drug. The Dex-b-PCL diblock copolymers were synthesized by end-to-end coupling of amino-terminated dextran and aldehyde-terminated poly(ɛ-caprolactone) and characterized by (1)H NMR spectra and gel permeation chromatography. The DOX-loaded Dex-b-PCL nanoparticles were prepared by a modified nanoprecipitation method and characterized by transmission electron microscopy and dynamic light scattering. In vitro release of DOX from DOX-Dex-b-PCL nanoparticles showed a sustained release manner with certain amount of burst release in the first 9h. In vitro cytotoxicity test of DOX-Dex-b-PCL nanoparticles against SH-SY5Y cells showed that DOX is still pharmacologically active after drug loading. The fluorescence imaging results showed that DOX-Dex-b-PCL nanoparticles could be easily uptaken by SH-SY5Y cells. These results indicate that DOX-Dex-b-PCL nanoparticles may be a promising nanocarrier for DOX.
authors.affiliation: College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Bengang
authors.initials: B
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Chongzhi
authors.initials: C
authors.lastname: Wang
authors.forename: Xiqun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002208:Caproates / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D003911:Dextrans / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage* / Q000494:pharmacology; D004337:Drug Carriers / Q000008:administration & dosage; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems / Q000379:methods; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007783:Lactones / Q000008:administration & dosage* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D002208:Caproates; D003911:Dextrans; D004337:Drug Carriers; D007783:Lactones; C121056:caprolactone; D004317:Doxorubicin