title: Fast spore breaking by superheating.
abstract: Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 °C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 °C resulted in the release of DNA within 20 s.
authors.affiliation: Korean Institute of Science and Technology in Europe (KIST Europe GmbH), Saarbruecken, Germany.
authors.forename: Adam
authors.initials: A
authors.lastname: Přibylka
authors.forename: Ana Vanessa
authors.initials: AV
authors.lastname: Almeida
authors.forename: Matthias O
authors.initials: MO
authors.lastname: Altmeyer
authors.forename: Jan
authors.initials: J
authors.lastname: Petr
authors.forename: Juraj
authors.initials: J
authors.lastname: Sevčík
authors.forename: Andreas
authors.initials: A
authors.lastname: Manz
authors.forename: Pavel
authors.initials: P
authors.lastname: Neužil
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry*; D006358:Hot Temperature; D046210:Microfluidic Analytical Techniques; D013171:Spores, Bacterial / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial