title: Effect of ouabain on growth of skin explants in organotypic culture.
abstract: Organotypic culture was used to study the effects of glycoside ouabain and its aglycone ouaba-genin on the growth of skin explants from 10-12-day chicken embryos. The tested agents demonstrated dose-dependent inhibition of skin growth. The effective concentrations imply that ouabain and ouabagenin mediate their inhibitory effect on the skin growth via interaction with α1-isoform of Na(+),K(+)-ATPase that plays mostly the role of ionic pump.
authors.affiliation: Laboratory of Excitable Membrane Physiology, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Penniyaynen
authors.forename: A V
authors.initials: AV
authors.lastname: Kipenko
authors.forename: E V
authors.initials: EV
authors.lastname: Lopatina
authors.forename: B V
authors.initials: BV
authors.lastname: Krylov
mesh_terms: D000818:Animals; D002642:Chick Embryo; D010042:Ouabain / Q000031:analogs & derivatives / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; C008721:ouabagenin