title: Increasing CCL5/CCR5 on CD4+ T cells in peripheral blood of oral lichen planus.
abstract: Oral lichen planus (OLP) is a T cell-mediated autoimmune disease of oral mucosa, in which T helper 1 (Th1) cells are greatly involved. Chemokine CCL5 is required for T cells infiltration and activation. CCR5, one of its receptors, specifically expressed on Th1 cells among CD4(+) T cells, can be up-regulated by Th1 cytokines like interleukin2 (IL-2) and interferon-gamma (IFN-γ), and down-regulated by Th2 cytokines like IL-4. The present study aimed to determine whether CCL5 and CCR5 had effects on the immune response of OLP. We analyzed the proportion of CCR5(+)CD4(+) T cells in CD4(+) T cells using flow cytometry and the serum levels of CCL5, IL-2, IFN-γ, and IL-4 with ELISA. MicroRNA-125a (miR-125a), a blocker of CCL5, was examined with RT-PCR. The results showed both the serum CCL5 and the percentage of CCR5(+)CD4(+) T cells elevated in OLP patients. Serum IL-2 and IFN-γ increased in OLP patients, but IL-4 decreased. MiR-125a was down-regulated in OLP patients, and there was a negative correlation between miR-125a content and the OLP severity which was measured with a RAE (reticular, atrophic and erosive lesion) scoring system. In conclusion, increasing CCl5/CCR5 might participate in the immune response of OLP. Th1-type cytokines environment presented in OLP probably performed as a magnifier for the CCR5. Moreover, miR-125a might be a candidate biomarker to estimate the severity of OLP.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Ji-Li
authors.initials: JL
authors.lastname: Cui
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Liang
authors.forename: Rui
authors.initials: R
authors.lastname: Lu
authors.forename: Ge-Fei
authors.initials: GF
authors.lastname: Du
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018946:Chemokine CCL5 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D017676:Lichen Planus, Oral / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000378:metabolism*; D012720:Severity of Illness Index; D015398:Signal Transduction; D055815:Young Adult
chemical_list: D018946:Chemokine CCL5; D007376:Interleukin-2; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D019713:Receptors, CCR5; D015847:Interleukin-4; D007371:Interferon-gamma