title: Heat shock protein 90 of Bonamia ostreae: characterization and possible correlation with infection of the flat oyster, Ostrea edulis.
abstract: In this study, we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis hemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae-infected hemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in hemocyte invasion. Our results inform the molecular basis that governs B. ostreae-O. edulis interactions.
authors.affiliation: IFREMER, Laboratoire de Génétique et Pathologie, Avenue de Mus de Loup, 17390, La Tremblade, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Prado-Alvarez
authors.forename: Bruno
authors.initials: B
authors.lastname: Chollet
authors.forename: Yann
authors.initials: Y
authors.lastname: Couraleau
authors.forename: Benjamin
authors.initials: B
authors.lastname: Morga
authors.forename: Isabelle
authors.initials: I
authors.lastname: Arzul
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D041602:Haplosporida / Q000378:metabolism* / Q000472:pathogenicity*; D010125:Ostreidae / Q000469:parasitology*
chemical_list: D018076:DNA, Complementary; D018841:HSP90 Heat-Shock Proteins