title: Molecular cytological characterization of two novel durum--Thinopyrum intermedium partial amphiploids with resistance to leaf rust, stem rust and Fusarium head blight.
abstract: Thinopyrum intermedium, a wild relative of wheat, is an excellent source of disease resistance. Two novel partial amphiploids, 08-47-50 and 08-53-55 (2n = 6x = 42), were developed from wide crosses between durum wheat and Th. intermedium. Meiotic analysis showed that pollen mother cells of the two partial amphiploids formed an average 20.49 bivalents for 08-47-50 and 20.67 bivalents for 08-53-55, indicating that they are basically cytologically stable. GISH analysis revealed that the two partial amphiploids carried different chromosome compositions. 08-47-50 had fourteen chromosomes from Th. intermedium and its alien chromosomes included six St-, four E(e) - and four E(e)-St translocated chromosomes, whereas 08-53-55 had four St- and ten E(e)-St translocated chromosomes. Fungal disease evaluation indicated that both partial amphiploids had a high level of resistance to FHB, leaf rust and stem rust race Ug99. These two novel partial amphiploids with multiple disease resistance could be used as a new source of multiple disease resistance in bread wheat and durum wheat breeding programs.
authors.affiliation: Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Zeng
authors.forename: W
authors.initials: W
authors.lastname: Cao
authors.forename: G
authors.initials: G
authors.lastname: Fedak
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.forename: B
authors.initials: B
authors.lastname: McCallum
authors.forename: T
authors.initials: T
authors.lastname: Fetch
authors.forename: A
authors.initials: A
authors.lastname: Xue
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D015877:Miosis; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*