title: [Characterization of cold-adapted influenza strain A/HongKong/1/68/162/35 as a potential donor of attenuation and high reproduction].
abstract: Live and inactivated vaccines are currently produced using virus reassortants originating from various gene donors of internal proteins. Based on the pandemic virus A/Hong Kong/1/68 (H3N2), a cold-adapted thermo-sensitive strain A/Hong Kong/1/68/162/35 was generated. It is distinguished for its high reproductive capacity (9-9.5 lg EID50), and hemagglutinating activity (1:1024-1:2048). The strain has ts and ca phenotype: reproductive capacity at t = 39 degrees C is 1.0 lg EID50; at t = 26 degrees C, 8.5 lg EID50. A total of 16 mutations have emerged from comprehensive sequencing of the virus genome. Among them 10 mutations were located in the genes of polymerase complex and NP, with respective amino-acid substitutions. The stability of strain characteristics, such as attenuation to humans and high reproductive capacity, were confirmed by repeated sequencing of the genome after tenfold passing of the virus in chicken embryos. Reassortants of the strain A/Hong Kong/1/68/162/35 with the wild-type viruses have inherited useful features of donor virus.
authors.forename: L M
authors.initials: LM
authors.lastname: Tsybalova
authors.forename: N E
authors.initials: NE
authors.lastname: Gorev
authors.forename: M V
authors.initials: MV
authors.lastname: Potapchuk
authors.forename: I A
authors.initials: IA
authors.lastname: Repko
authors.forename: A V
authors.initials: AV
authors.lastname: Korotkov
authors.forename: M V
authors.initials: MV
authors.lastname: Sergeeva
authors.forename: A B
authors.initials: AB
authors.lastname: Komissarov
authors.forename: M M
authors.initials: MM
authors.lastname: Pisareva
authors.forename: V V
authors.initials: VV
authors.lastname: Kuznetsov
authors.forename: M P
authors.initials: MP
authors.lastname: Grudinin
authors.forename: O I
authors.initials: OI
authors.lastname: Kiselev
mesh_terms: D003080:Cold Temperature; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics*; D007251:Influenza, Human / Q000235:genetics / Q000517:prevention & control / Q000821:virology; D009154:Mutation; D016865:Reassortant Viruses / Q000235:genetics; D013696:Temperature; D014613:Vaccines, Attenuated; D014764:Viral Proteins / Q000235:genetics
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D014764:Viral Proteins