title: [Study on treatment effect and mechanism of Hirsutella sinensis mycelium in idiopathic pulmonary fibrosis in rats].
abstract: OBJECTIVE
  To study and discuss the effect and mechanism of Hirsutella sinensis mycelium (HSM) on idiopathic pulmonary fibrosis in rats.
  
  
  METHODS
  Forty Wistar rats were divided into five groups: the normal control group, the model control group, the high-dose group (1.0 g x kg(-1) HSM), the low-dose group (0.5 g x kg(-1) HSM), and the positive control group (10 mg x kg(-1) hydrocortisone). In addition to rats in the normal control group, the pulmonary fibrosis model was established by injecting 5 mg x kg(-1) bleomycin into rat tracheas for consecutively 28 days, in order to observe their lung function, lung tissue hydroxyproline, cytokines and pathology.
  
  
  RESULTS
  After rats were administered with HSM, 0.5 g x kg(-1) and 1.0 g x kg(-1) HSM could significantly decrease lung index and hydroxyproline content (P<0.01), while notably improving pulmonary function, alveolus inflammation and fibrosis degree (P<0.05, P<0.01); 1.0 g x kg(-1) HSM could decrease significantly protein expressions of TNF-alpha, IL-1beta and TGF-beta1 in lung tissues, while increasing significantly protein expressions of IFN-gamma (P<0.05).
  
  
  CONCLUSIONS
  HSM have better effect in treating idiopathic pulmonary fibrosis in rats. Its treatment effect and mechanism are related to the regulation of TNF-alpha, IL-1beta and TGF-beta1 and IFN-gamma imbalance.
authors.affiliation: Animal Laboratory Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China. sqy133@126.com
authors.forename: Qi-Yang
authors.initials: QY
authors.lastname: Shou
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Fu
authors.forename: Li-Zong
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Cai
authors.forename: Fang-Ming
authors.initials: FM
authors.lastname: Chen
authors.forename: Min-Li
authors.initials: ML
authors.lastname: Chen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D006999:Hypocreales / Q000737:chemistry* / Q000254:growth & development; D054990:Idiopathic Pulmonary Fibrosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha