title: [Sperm DNA integrity of infertile males with hepatitis B virus infection].
abstract: OBJECTIVE
  To investigate sperm DNA integrity in male infertility patients with hepatitis B virus (HBV) infection.
  
  
  METHODS
  This study included 90 infertile men with HBV infection (group A), 82 infertile men without HBV infection (group B) and 70 normal fertile men (group C). We detected sperm DNA integrity among the subjects, including DNA fragmentation index (DFI) and high DNA stainability (HDS), by sperm chromatin structure assay (SCSA), and compared them among the three groups.
  
  
  RESULTS
  DFI was higher in group A ([28.17 +/- 13.06]%) than in B ([26.64 +/- 9.79]%) and C ([15.67 +/- 4.73]%), significantly higher in A and B than in C (P < 0.05) but with no significant difference between A and B (P > 0.05). HDS was higher in group A ([10.83 +/- 5.601]%) than in B ([9.04 +/- 3.48]%) and C ([8.04-2.25]%), with significant difference between A and C (P < 0.05).
  
  
  CONCLUSIONS
  Sperm DNA integrity of infertile males is significantly different from that of normal fertile men, and infertility with HBV infection further impairs sperm DNA, which is manifested by abnormal sperm nuclear maturity.
authors.affiliation: Department of Urology, The First Peoples Hospital of Foshan, Foshan, Guangdong 528000, China. tianqin02@163.com
authors.forename: Tian-Qin
authors.initials: TQ
authors.lastname: Deng
authors.forename: Yong-Han
authors.initials: YH
authors.lastname: Huang
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Zhen
authors.forename: Jian-Tang
authors.initials: JT
authors.lastname: Lu
authors.forename: Ying-Chang
authors.initials: YC
authors.lastname: Li
authors.forename: Xing-Yin
authors.initials: XY
authors.lastname: Tan
authors.forename: Xiong-Chun
authors.initials: XC
authors.lastname: Liu
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Ruan
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Zhang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002843:Chromatin; D004247:DNA / Q000235:genetics*; D004249:DNA Damage; D006509:Hepatitis B / Q000473:pathology*; D006515:Hepatitis B virus; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000821:virology*; D008297:Male; D013076:Sperm Count; D013094:Spermatozoa / Q000473:pathology; D055815:Young Adult
chemical_list: D002843:Chromatin; D004247:DNA