title: Human tissue kallikreins 3 and 5 can act as plasminogen activator releasing active plasmin.
abstract: Human tissue kallikreins (KLKs) are a group of serine proteases found in many tissues and biological fluids and are differentially expressed in several specific pathologies. Here, we present evidences of the ability of these enzymes to activate plasminogen. Kallikreins 3 and 5 were able to induce plasmin activity after hydrolyzing plasminogen, and we also verified that plasminogen activation was potentiated in the presence of glycosaminoglycans compared with plasminogen activation by tPA. This finding can shed new light on the plasminogen/plasmin system and its involvement in tumor metastasis, in which kallikreins appear to be upregulated.
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, SP, Brazil.
authors.forename: Lucas R
authors.initials: LR
authors.lastname: de Souza
authors.forename: Pollyana
authors.initials: P
authors.lastname: M Melo
authors.forename: Thaysa
authors.initials: T
authors.lastname: Paschoalin
authors.forename: Adriana K
authors.initials: AK
authors.lastname: Carmona
authors.forename: Marcia
authors.initials: M
authors.lastname: Kondo
authors.forename: Izaura Y
authors.initials: IY
authors.lastname: Hirata
authors.forename: Michael
authors.initials: M
authors.lastname: Blaber
authors.forename: Ivarne
authors.initials: I
authors.lastname: Tersariol
authors.forename: Joyce
authors.initials: J
authors.lastname: Takatsuka
authors.forename: Maria A
authors.initials: MA
authors.lastname: Juliano
authors.forename: Luiz
authors.initials: L
authors.lastname: Juliano
authors.forename: Roseli A
authors.initials: RA
authors.lastname: Gomes
authors.forename: Luciano
authors.initials: L
authors.lastname: Puzer
mesh_terms: D000595:Amino Acid Sequence; D016367:Baculoviridae / Q000235:genetics; D002863:Chromogenic Compounds / Q000737:chemistry; D057075:Enzyme Assays; D005341:Fibrinolysin / Q000737:chemistry*; D006801:Humans; D007610:Kallikreins / Q000737:chemistry*; D007700:Kinetics; D008969:Molecular Sequence Data; D010958:Plasminogen / Q000737:chemistry*; D017395:Plasminogen Activator Inhibitor 1 / Q000737:chemistry*; D059748:Proteolysis; D011994:Recombinant Proteins / Q000737:chemistry; D012996:Solutions; D010959:Tissue Plasminogen Activator / Q000737:chemistry*
chemical_list: D002863:Chromogenic Compounds; D017395:Plasminogen Activator Inhibitor 1; D011994:Recombinant Proteins; D012996:Solutions; D010958:Plasminogen; C400147:KLK5 protein, human; D007610:Kallikreins; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin