title: Expression analysis of thrombospondin 2 in prostate cancer and benign prostatic hyperplasia.
abstract: Thrombospondin 2 (TSP2) is a protein with important roles in different tumor types, mainly related to tumor inhibition. However, there are limiting data regarding TSP2 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH). We aimed to investigate TSP2 transcript and protein expression in tumoral and non-tumoral prostate tissues and cell lines, and its implications for PCa diagnosis and progression. TSP2 transcript expression was evaluated by real time PCR in PCa and BPH tissue samples and in tumoral and non-tumoral cell lines. TSP2 protein expression analysis was conducted by immunohistochemistry in a tissue microarray (TMA) containing PCa and BPH tissue samples. TSP2 transcript was down-regulated in PCa tissue samples and cell lines, when compared to BPH and non-tumoral samples (P<0.01). Receiver Operating Curve (ROC) analysis demonstrated that TSP2 transcript levels can better distinguish PCa from BPH tissue samples (P<0.01) than serum PSA levels (P=0.299). TSP2 protein expression has been observed in the cytoplasm of both PCa and BPH epithelial and stromal compartments. TSP2 stromal staining scores were significantly lower in PCa than in BPH tissues (P<0.01), while similar TSP2 epithelial staining patterns were observed in both diseases. Notably, the TSP2 epithelial staining score was significantly correlated to vascular invasion and biochemical recurrence in PCa tissue samples (P<0.05). Our data indicate that TSP2 is down-regulated at PCa tissues and cell lines, especially at stroma compartment, which could be related to PCa progression. TSP2 levels could potentially be applied for differential PCa and BPH diagnosis.
authors.affiliation: Programa de Carcinogênese Molecular/Programa de Pós Graduação Stricto Sensu em Oncologia do Instituto Nacional de Câncer, CPQ, Rio de Janeiro, RJ, Brazil.
authors.forename: A R
authors.initials: AR
authors.lastname: Matos
authors.forename: C M
authors.initials: CM
authors.lastname: Coutinho-Camillo
authors.forename: L C S
authors.initials: LC
authors.lastname: Thuler
authors.forename: F P
authors.initials: FP
authors.lastname: Fonseca
authors.forename: F A
authors.initials: FA
authors.lastname: Soares
authors.forename: E A
authors.initials: EA
authors.lastname: Silva
authors.forename: E R
authors.initials: ER
authors.lastname: Gimba
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000032:analysis; D018450:Disease Progression; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D011237:Predictive Value of Tests; D017430:Prostate-Specific Antigen / Q000097:blood; D011470:Prostatic Hyperplasia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D019699:Thrombospondins / Q000235:genetics* / Q000378:metabolism; D046888:Tissue Array Analysis; D000099262:Thrombospondin 2
chemical_list: D004273:DNA, Neoplasm; D017430:Prostate-Specific Antigen; D019699:Thrombospondins; D000099262:Thrombospondin 2