title: A comparative evaluation of whole blood total antioxidant capacity using a novel nitroblue tetrazolium reduction test in patients with periodontitis and healthy subjects: A randomized, controlled trial.
abstract: The investigation of disease-related oxidant-antioxidant imbalance is difficult due to the limited availability of specific biomarkers of oxidative stress, and the fact that measurement of individual antioxidant may give misleading picture because antioxidants work in concert through chain breaking reactions. Therefore, analysis of total antioxidant capacity may be the most relevant investigation. As the total blood is continuously exposed to oxidative stress, the aim of the current study was to investigate total blood antioxidant capacity in healthy and periodontitis patients by using novel Nitroblue Tetrazolium reduction test. The study was conducted on 30 non-smoking volunteers with age ranging between 18-40 years. They were categorized into two groups; chronic periodontitis group and healthy group, respectively. Total antioxidant capacity in whole blood was assessed using Nitroblue Tetrazolium reduction test. Results of the present study has shown that the total antioxidant capacity in whole blood in patients with periodontitis was significantly (P<0.005) lower than in control subjects. The reduced total blood antioxidant status in periodontitis subjects warrants further investigation as it may provide a mechanistic link between periodontal disease and several other free radical-associated chronic inflammatory diseases.
authors.affiliation: Department of Periodontics, M. S. Ramaiah Dental College, Bangalore, Karnataka, India.
authors.forename: Kranti
authors.initials: K
authors.lastname: Konuganti
authors.forename: Hema
authors.initials: H
authors.lastname: Seshan
authors.forename: Sameer
authors.initials: S
authors.lastname: Zope
authors.forename: Wilma Delphine
authors.initials: WD
authors.lastname: Silvia
keywords: Nitroblue tetrazolium reduction test; oxidative stress; total antioxidant capacity