title: Application of a europium complex, Eu(AA)3phen (AA=acrylic acid, phen=1,10-phenanthroline) as a spectroscopic probe and cleaving reagent of DNA.
abstract: A complex Eu(AA)3phen was synthesized and characterized by elemental analysis, IR spectroscopy and (1)H NMR. Interaction between Eu(AA)3phen and DNA was studied by UV/visible absorption spectroscopy, fluorescence spectrophotometer, circular-dichroism (CD) spectra and gel-electrophoresis measurements. Absorption spectral indicated that Eu(AA)3phen binding to DNA was an electrostatic mode, which was authenticated by the effect of ionic strength, thermal denaturation and fluorescence quenching experiments. The intrinsic binding constant Kb of Eu(AA)3phen was calculated to be 1.6×10(4) L mol(-1).
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Quan
authors.initials: Q
authors.lastname: Pan
authors.forename: Gong Wu
authors.initials: GW
authors.lastname: Song
mesh_terms: D000042:Absorption; D000179:Acrylates / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D005063:Europium / Q000737:chemistry*; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D010618:Phenanthrolines / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D000179:Acrylates; D007202:Indicators and Reagents; D010618:Phenanthrolines; D012996:Solutions; D005063:Europium; D004247:DNA; C036658:acrylic acid; C025205:1,10-phenanthroline