title: An improved fluorogenic substrate for the detection of alkaline phosphatase activity.
abstract: We designed a new alkaline phosphatase (ALP)-sensitive fluorogenic probe in which a self-immolative spacer group, p-hydroxybenzyl alcohol, is linked to a profluorogenic compound to improve substrate specificity. Enzymatic hydrolysis converts the fluorogenic substrate 1 to a highly fluorescent reporter 3, thus allowing for the fast and quantitative analysis of ALP activity with greatly increased affinity for the enzyme.
authors.affiliation: Department of Chemistry, Institute of Nanosensor and Biotechnology, Dankook University, 126 Jukjeon-dong, Yongin-si, Gyeonggi-do 448-701, Republic of Korea.
authors.forename: Jeesook
authors.initials: J
authors.lastname: Park
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000378:metabolism; D001555:Benzene Derivatives / Q000138:chemical synthesis / Q000737:chemistry; D004987:Ethers / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D055668:Photochemical Processes; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D001555:Benzene Derivatives; D004987:Ethers; D005456:Fluorescent Dyes; D000469:Alkaline Phosphatase