title: Comparative analysis of adiponectin isoform distribution in pregnant women with gestational diabetes mellitus and after delivery.
abstract: OBJECTIVE
  To evaluate the distribution of circulating adiponectin isoforms in pregnant women with gestational diabetes mellitus (GDM) and compare isoform distribution changes 12 months after delivery.
  University Hospital.
  
  
  METHODS
  Sixty-four consecutive pregnant women who underwent routine prenatal tests for GDM between 24 and 28 weeks of gestation. The study group included 36 women diagnosed with GDM, 30 of whom underwent re-testing for diabetes 12 months after delivery. The control group included 28 healthy pregnant women.
  
  
  METHODS
  Quantitative determination of total adiponectin and its isoforms in serum was performed with the multimeric adiponectin enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Total adiponectin concentration in women with GDM was significantly lower than in women without GDM. Among adiponectin isoforms, significantly lower concentrations of multimers and trimers in women with GDM were found in comparison to controls, while the concentration of hexamers did not differ between the groups. After delivery, a significant increase in total adiponectin and in the concentration of multimers and trimers was found in women with history of GDM. Additionally, we found that multimers and hexamers were negatively correlated with body mass index before and during gestation, as well as after delivery, and middle molecular weight isoforms in gestation were negatively correlated with birthweight.
  
  
  CONCLUSIONS
  Hypoadiponectinemia during GDM was characterized by a significant, reversible decrease in multimeric levels; thus this isoform seems to function in a cohesive way with total adiponectin. Negative correlations of adiponectin isomers with body mass index indicate that there is a possibility to normalize total adiponectin and prevent GDM.
authors.affiliation: Department of Endocrinology, Medical University, Lublin, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Matuszek
authors.forename: Agata
authors.initials: A
authors.lastname: Burska
authors.forename: Bożena
authors.initials: B
authors.lastname: Leszczyńska-Gorzelak
authors.forename: Helena
authors.initials: H
authors.lastname: Donica
authors.forename: Andrzej
authors.initials: A
authors.lastname: Nowakowski
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D001724:Birth Weight; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D016640:Diabetes, Gestational / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005215:Fasting; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D049590:Postpartum Period / Q000097:blood*; D011247:Pregnancy; D020033:Protein Isoforms / Q000097:blood; D012044:Regression Analysis
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D020033:Protein Isoforms
keywords: Adiponectin; adiponectin isoforms; body mass index; gestational diabetes mellitus; glucose tolerance; molecular weight