title: Milk fatty acid variability: effect of some candidate genes involved in lipid synthesis.
abstract: In this work, the genetic variation of milk FA was investigated in three different bovine breeds, the Jersey, the Piedmontese and the Valdostana, and at different lactation stages. All animals were genotyped for 21 Single Nucleotide Polymorphisms located within nine candidate genes involved in lipid synthesis: diacylglycerol acyltransferase 1 and 2 (DGAT1, 2); stearoyl-CoA desaturase (SCD); growth hormone receptor (GHR); fatty acid synthase (FASN); acyl-CoA dehydrogenase (ACAD); fatty acid binding protein (FABP4); lipoprotein lipase (LPL); and leptin gene (LEP). The highest milk-fat Jersey breed also showed the highest content of saturated FA. Throughout lactation, the breeds showed a similar variation in the FA, with a decrease in the short-chain, this was accompanied by a general increase in the long chain FA at the end of lactation. The increase in long chain saturated FA was particularly evident in the case of the Jersey. The effect of SCD gene on the C14 desaturation index was confirmed; the DGAT1 gene was polymorphic only in the Jersey breed, but its effect was confirmed only on milk fat content; three further potential candidate genes were identified: first, the FABP4 gene, which was found to influence medium and long chain FA in all the breeds, but not the desaturation indices; second, the FASN gene, which was found to influence the amount of PUFA in the Piedmontese and the Valdostana, and third, the LPL gene, which was found to affect fat content in the Piedmontese.
authors.affiliation: Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Marchitelli
authors.forename: Giovanna
authors.initials: G
authors.lastname: Contarini
authors.forename: Giovanna
authors.initials: G
authors.lastname: De Matteis
authors.forename: Alessandra
authors.initials: A
authors.lastname: Crisà
authors.forename: Lorraine
authors.initials: L
authors.lastname: Pariset
authors.forename: Maria Carmela
authors.initials: MC
authors.lastname: Scatà
authors.forename: Gennaro
authors.initials: G
authors.lastname: Catillo
authors.forename: Francesco
authors.initials: F
authors.lastname: Napolitano
authors.forename: Bianca
authors.initials: B
authors.lastname: Moioli
mesh_terms: D000818:Animals; D002417:Cattle; D051048:Diacylglycerol O-Acyltransferase / Q000032:analysis / Q000235:genetics; D064429:Fatty Acid Synthases / Q000032:analysis / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis* / Q000235:genetics*; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000235:genetics; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D007774:Lactation / Q000235:genetics; D008055:Lipids / Q000096:biosynthesis; D008071:Lipoprotein Lipase / Q000032:analysis / Q000235:genetics; D008892:Milk / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013045:Species Specificity; D013230:Stearoyl-CoA Desaturase / Q000235:genetics
chemical_list: D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D013230:Stearoyl-CoA Desaturase; D051048:Diacylglycerol O-Acyltransferase; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase