title: Usage of plasmid vector carrying vegf and fgf2 genes after spinal cord injury in rats.
abstract: Using rat model of spinal cord contusion injury at TVIII, we compared the effectiveness of immediate single transplantation of human mononuclear umbilical cord blood cells transfected with pBud-VEGF-FGF2 plasmid and immediate direct injection of the same plasmid into the lesion area. The results suggest that the delivery of therapeutic genes vegf and fgf2 in cells is more effective than direct injection of plasmid DNA with the same genes (judging from the number of myelinated fibers). Better tissue preservation and motor function recovery in experiments with direct injection of plasmid pBud-VEGF-FGF2 suggest that direct gene therapy seems to be an effective additional procedure to the method of gene delivery with transfected stem and progenitor cells.
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Research Center of the Russian Academy of Sciences, Russia. gulnara_kzn@rambler.ru
authors.forename: G F
authors.initials: GF
authors.lastname: Shaymardanova
authors.forename: Y O
authors.initials: YO
authors.lastname: Mukhamedshina
authors.forename: I I
authors.initials: II
authors.lastname: Salafutdinov
authors.forename: A A
authors.initials: AA
authors.lastname: Rizvanov
authors.forename: Yu A
authors.initials: YA
authors.lastname: Chelyshev
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D008297:Male; D010957:Plasmids / Q000235:genetics*; D051381:Rats; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2