title: Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB.
abstract: ACTN4 is an actin-binding protein that participates in cytoskeleton organisation. It resides both in the cytoplasm and nucleus and physically associates with various transcription factors. Here, we describe an effect of ACTN4 expression on transcriptional activity of the RelA/p65 subunit of NF-kB. We demonstrate that ACTN4 enhances RelA/p65-dependant expression of c-fos, MMP-3 and MMP-1 genes, but it does not affect TNC, ICAM1 and FN1 expression. Importantly, actin-binding domains of ACTN4 are not critical for the nuclear translocation and co-activation of RelA/p65- dependent transcription. Collectively, our data suggest that in the nucleus, ACTN4 functions as a selective transcriptional co-activator of RelA/p65.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av., 4, St. Petersburg, Russia.
authors.forename: Vasilisa
authors.initials: V
authors.lastname: Aksenova
authors.forename: Lidia
authors.initials: L
authors.lastname: Turoverova
authors.forename: Mikhail
authors.initials: M
authors.lastname: Khotin
authors.forename: Karl-Eric
authors.initials: KE
authors.lastname: Magnusson
authors.forename: Eugene
authors.initials: E
authors.lastname: Tulchinsky
authors.forename: Gerry
authors.initials: G
authors.lastname: Melino
authors.forename: George P
authors.initials: GP
authors.lastname: Pinaev
authors.forename: Nickolai
authors.initials: N
authors.lastname: Barlev
authors.forename: Dmitri
authors.initials: D
authors.lastname: Tentler
mesh_terms: D000185:Actinin / Q000235:genetics* / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D045744:Cell Line, Tumor; D057809:HEK293 Cells; D006801:Humans; D020782:Matrix Metalloproteinases / Q000096:biosynthesis / Q000235:genetics; D055503:Protein Multimerization; D051996:Transcription Factor RelA / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C111453:ACTN4 protein, human; D000199:Actins; D051996:Transcription Factor RelA; D000185:Actinin; D020782:Matrix Metalloproteinases