title: Stability and mechanical evaluation of bovine pericardium cross-linked with polyurethane prepolymer in aqueous medium.
abstract: The present study investigates the potential use of non-catalyzed water-soluble blocked polyurethane prepolymer (PUP) as a bifunctional cross-linker for collagenous scaffolds. The effect of concentration (5, 10, 15 and 20%), time (4, 6, 12 and 24 h), medium volume (50, 100, 200 and 300%) and pH (7.4, 8.2, 9 and 10) over stability, microstructure and tensile mechanical behavior of acellular pericardial matrix was studied. The cross-linking index increased up to 81% while the denaturation temperature increased up to 12 °C after PUP crosslinking. PUP-treated scaffold resisted the collagenase degradation (0.167±0.14 mmol/g of liberated amine groups vs. 598±60 mmol/g for non-cross-linked matrix). The collagen fiber network was coated with PUP while viscoelastic properties were altered after cross-linking. The treatment of the pericardial scaffold with PUP allows (i) different densities of cross-linking depending of the process parameters and (ii) tensile properties similar to glutaraldehyde method.
authors.affiliation: Universidad de Guanajuato, División de Ciencias e Ingenierías, Depto. de Ingenierías Química, Electrónica y Biomédica, Loma del Bosque 103, 37150, León, Gto.,Mexico.
authors.forename: Birzabith
authors.initials: B
authors.lastname: Mendoza-Novelo
authors.forename: Diego I
authors.initials: DI
authors.lastname: Alvarado-Castro
authors.forename: José L
authors.initials: JL
authors.lastname: Mata-Mata
authors.forename: Juan V
authors.initials: JV
authors.lastname: Cauich-Rodríguez
authors.forename: Arturo
authors.initials: A
authors.lastname: Vega-González
authors.forename: Eduardo
authors.initials: E
authors.lastname: Jorge-Herrero
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Rojo
authors.forename: Gustavo V
authors.initials: GV
authors.lastname: Guinea
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D055119:Elastic Modulus / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000648:ultrastructure; D005976:Glutaral / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008422:Materials Testing; D055595:Mechanical Phenomena / Q000187:drug effects*; D010496:Pericardium / Q000187:drug effects* / Q000648:ultrastructure; D010758:Phosphorus / Q000378:metabolism; D011140:Polyurethanes / Q000494:pharmacology*; D013314:Stress, Mechanical; D013696:Temperature; D013718:Tensile Strength / Q000187:drug effects; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D011140:Polyurethanes; D014867:Water; D010758:Phosphorus; D002118:Calcium; D005976:Glutaral