title: Extrachromosomal inducible expression.
abstract: Inducible expression systems are very convenient for proteins that induce strong side effects such as retardation of growth or development and are essential for the expression of toxic proteins. In this chapter we describe the doxycycline-inducible expression system, optimized for the controlled expression in. Two types of inducible plasmids are presented, in which transcription is induced by either adding or removing doxycycline, respectively. Detailed protocols are provided for the construction of the plasmids and the inducible expression of the target protein.
authors.affiliation: Beatson Institute for Cancer Research, Glasgow, UK. d.veltman@beatson.gla.ac.uk
authors.forename: Douwe M
authors.initials: DM
authors.lastname: Veltman
authors.forename: Peter J M
authors.initials: PJ
authors.lastname: Van Haastert
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D015533:Transcriptional Activation; D014169:Transformation, Bacterial
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins