title: TEM-187, a new extended-spectrum β-lactamase with weak activity in a Proteus mirabilis clinical strain.
abstract: A Proteus mirabilis clinical strain (7001324) was isolated from urine sample of a patient hospitalized in a long-term-care facility. PCR and cloning experiments performed with this strain identified a novel TEM-type β-lactamase (TEM-187) differing by four amino acid substitutions (Leu21Phe, Arg164His, Ala184Val, and Thr265Met) from TEM-1. This characterization provides further evidence for the diversity of extended-spectrum β-lactamases (ESBL) produced by P. mirabilis and for their potential spread to other Enterobacteriaceae due to a lack of sensitive detection methods used in daily practice.
authors.affiliation: Service de Bactériologie-Hygiène, CHU de Nantes, Institut de Biologie, Nantes, France. stephane.corvec@chu-nantes.fr
authors.forename: Stéphane
authors.initials: S
authors.lastname: Corvec
authors.forename: Racha
authors.initials: R
authors.lastname: Beyrouthy
authors.forename: Lise
authors.initials: L
authors.lastname: Crémet
authors.forename: Guillaume Ghislain
authors.initials: GG
authors.lastname: Aubin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Robin
authors.forename: Richard
authors.initials: R
authors.lastname: Bonnet
authors.forename: Alain
authors.initials: A
authors.lastname: Reynaud
mesh_terms: D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011512:Proteus Infections / Q000188:drug therapy / Q000382:microbiology; D011513:Proteus mirabilis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases