title: Measurement of the methemoglobin concentration using Raman spectroscopy.
abstract: Methemoglobin concentration is an important pathophysiological biomarker, reflecting the oxygen-carrying and oxygen-releasing capabilities of hemoglobin (Hb). Raman spectroscopy is used to develop a novel technique for determining the methemoglobin concentration. Raman activity combined with two-dimensional correlation analysis is an attractive method for investigating Hb oxidation, exhibiting several relevant peaks in the range of 1200-1650 cm(-1). Methemoglobin concentration is estimated by measuring the intensity of Raman peaks in the ranges of 1210-1230 cm(-1) and 1340-1380 cm(-1) with 785-nm excitation. The correlation between Raman-based methemoglobin concentration estimations and the methemoglobin concentration measured using spectrophotometry was highly significant. These results suggest the potential of Raman spectroscopy as a new quantitative approach to determine the methemoglobin concentration.
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P. R. China.
authors.forename: Mingzi
authors.initials: M
authors.lastname: Lu
authors.forename: Lian
authors.initials: L
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Guoxing
authors.initials: G
authors.lastname: You
authors.forename: Xuemei
authors.initials: X
authors.lastname: Kan
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry*; D008706:Methemoglobin / Q000032:analysis*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D012015:Reference Standards; D012996:Solutions; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman
chemical_list: D006454:Hemoglobins; D012996:Solutions; D008706:Methemoglobin; D010100:Oxygen