title: The p53 protein does not facilitate adenovirus type 5 replication in normal human cells.
abstract: Although several adenovirus type 5 (Ad5) proteins prevent deleterious consequences of activation of p53, it has been reported that viral replication proceeds more efficiently when human tumor cells produce wild-type compared to mutant p53. We have now exploited RNA interference and lentiviral vectors to achieve essentially complete knockdown of p53 in normal human cells: no effects on the kinetics or efficiency of viral gene expression or production of infectious particles were observed.
authors.affiliation: Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Princeton, New Jersey, USA.
authors.forename: Jasdave S
authors.initials: JS
authors.lastname: Chahal
authors.forename: S J
authors.initials: SJ
authors.lastname: Flint
mesh_terms: D000260:Adenoviruses, Human / Q000502:physiology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000821:virology; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D006801:Humans; D034622:RNA Interference; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D016159:Tumor Suppressor Protein p53