title: Cytotoxic and anti-inflammatory eunicellin-based diterpenoids from the soft coral Cladiella krempfi.
abstract: Five new eunicellin-based diterpenoids, krempfielins E-I (1-5) and seven known compounds (6-12) were isolated from the organic extract of a Taiwanese soft coral Cladiella krempfi. The structures of the new metabolites were elucidated on the basis of extensive spectroscopic analysis. Metabolites 5, 6, 10 and 12 were shown to exhibit cytotoxicity against a limited panel of cancer cell lines. Furthermore, compounds 6 and 10 could potently inhibit the accumulation of the pro-inflammatory iNOS protein, and 6 and 12 could significantly reduce the expression of COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan. sheu@mail.nsysu.edu.tw
authors.forename: Chi-Jen
authors.initials: CJ
authors.lastname: Tai
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Huang
authors.forename: Ming-Shyan
authors.initials: MS
authors.lastname: Huang
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Dai
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000378:metabolism*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D013057:Spectrum Analysis; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D004224:Diterpenes; D008070:Lipopolysaccharides; C476503:eunicellin; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2