title: Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function: a potential for the vascular function biomarker?
abstract: Abstract We examined the presence of KATP channel subunits, Kir6.1 and SUR2B, mRNAs in the blood and vascular function in healthy volunteers (41 males, 34 females). Real-time reverse transcriptase (RT)-PCR threshold cycles (Ct) was used as an indicator of mRNA levels. Baseline skin perfusion and the post-occlusion reactive hyperemia response exhibited a significant positive correlation with Ct for Kir6.1. There was no correlation between Kir6.1 Ct and brachial artery flow-mediated dilatation. Gender had no influence on relationships between blood Kir6.1 Ct and vascular function. We conclude that blood Kir6.1 mRNA levels could be potentially used as a biomarker of the vascular function.
authors.affiliation: Medical Research Institute, Division of Cardiovascular and Diabetic Medicine, Ninewells Hospital & Medical School, University of Dundee, Dundee DD1 9SY, UK.
authors.forename: Faisel
authors.initials: F
authors.lastname: Khan
authors.forename: Wen Ling
authors.initials: WL
authors.lastname: Choong
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Du
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Jovanović
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000097:blood* / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D001916:Brachial Artery / Q000378:metabolism*; D004106:Dilatation; D005260:Female; D015870:Gene Expression; D006801:Humans; D006940:Hyperemia / Q000097:blood* / Q000235:genetics; D054086:KATP Channels / Q000097:blood* / Q000235:genetics; D008297:Male; D008833:Microcirculation; D024661:Potassium Channels, Inwardly Rectifying / Q000097:blood* / Q000235:genetics; D020033:Protein Isoforms / Q000097:blood / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards; D011955:Receptors, Drug / Q000097:blood* / Q000235:genetics; D012737:Sex Factors; D064233:Sulfonylurea Receptors; D055815:Young Adult
chemical_list: D018528:ATP-Binding Cassette Transporters; D015415:Biomarkers; D054086:KATP Channels; D024661:Potassium Channels, Inwardly Rectifying; D020033:Protein Isoforms; D011955:Receptors, Drug; D064233:Sulfonylurea Receptors; C092520:uK-ATP-1 potassium channel