title: Molecular detection and genomic characterization of Torque teno sus virus 1 and 2 from domestic pigs in central China.
abstract: In the present study, Torque teno sus viruses (TTSuVs) were detected in tissue and blood samples obtained from domestic pigs in central China, and complete genomes of TTSuVs were characterized. A total of three tissue samples (3/20, 15 %) from post-weaning multisystemic wasting syndrome-affected pigs and 30 blood samples (30/40, 75 %) from healthy pigs were positive for Torque teno sus virus 1 (TTSuV1) and/or 2 (TTSuV2). Two TTSuV strains (TTV1Hn54 and TTV2Hn93) comprising 2,794 and 2,875 nucleotides, respectively, each had four open reading frames (ORFs) and the untranslated region with TATA box and GC-rich region. Genomic sequence of TTV2Hn93 strain was unique in length compared with other TTSuV2 genomic sequences. Interestingly, three rolling-circle replication (RCR) motif-IIIs (YXXK) which were located at amino acid (aa) position 166-169, 328-331, and 379-382, respectively, were found in the ORF1 of TTV1Hn54. Two RCR motif-IIIs (YXXK) at the aa position 105-108 and 480-483 respectively, were also identified in the ORF1 of TTV2Hn93. Phylogenetic tree based on complete genomes showed that TTV1Hn54 strain was designated into type TTSuV1b and had a slight high sequence identity of 91 % with the Canada strain (JQ120664). TTV2Hn93 strain was classified into subtype TTSuV2d and shared the highest identity (97 %) with the Spain strain (GU570207).
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Nongye Road, Zhengzhou, 450002, Henan, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Lin-Qing
authors.initials: LQ
authors.lastname: Wang
authors.forename: Yu-Yang
authors.initials: YY
authors.lastname: Wu
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Chao
authors.forename: Quan-Wei
authors.initials: QW
authors.lastname: Lu
authors.forename: Zhan-Yong
authors.initials: ZY
authors.lastname: Wei
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Cui
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Chen
mesh_terms: D020121:5' Untranslated Regions; D020816:Amino Acid Motifs; D000825:Animal Structures / Q000821:virology; D000818:Animals; D001769:Blood / Q000821:virology; D002681:China; D017925:Circovirus / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000821:virology*; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013552:Swine; D016385:TATA Box
chemical_list: D020121:5' Untranslated Regions; D004279:DNA, Viral