title: [Protective effects of hepatocyte growth factor on hypoxic human pulmonary microvascular endothelial cells].
abstract: OBJECTIVE
  To investigate the protective effects of hepatocyte growth factor (HGF) on hypoxic human pulmonary microvascular endothelial cells (HPMECs).
  
  
  METHODS
  HPMECs were cultured in vitro, and the hypoxic model was established by the physical method. Cells were divided into 4 groups: the control group, the hypoxic group, HGF group, and phytohemagglutinin (PHA) group. The 7(th) generation of HPMECs was evaluated by the method of immunocytochemistry. The persistence rate of HPMECs was measured by MTT assay and the adhesive cells were counted by the microscopy. The expression of intercellular adhesion molecule-1 (ICAM-1) protein was determined by immunofluorescence staining.
  
  
  RESULTS
  The adherence percentage of cells significantly decreased after hypoxia, whereas the expression of the ICAM-1 protein was significantly higher in the hypoxia group than in control group (P<0.01). Compared with the hypoxia group, the persistence and adherence percentage of cells in the HGF group significantly increased (P<0.01), whereas the expression of the ICAM-1 protein significantly dropped (P<0.01). In the PHA group, the persistence and adhesion rate were significantly different from those in the hypoxia group and HGF group (P<0.01), and the expression of the ICAM-1 protein increased significantly (P<0.01).
  
  
  CONCLUSIONS
  HGF could inhibit the hypoxic damage of HPMECs by decreasing the persistence and the adhesive capacity of these cells and inducing the expression of ICAM-1.
authors.affiliation: Department of Respiratory Diseases, South Building of Chinese PLA General Hospital, Beijing, China.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Guo
authors.forename: Long-xiang
authors.initials: LX
authors.lastname: Su
authors.forename: Ya-juan
authors.initials: YJ
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xue-ge
authors.initials: XG
authors.lastname: Jiang
authors.forename: Chang-ting
authors.initials: CT
authors.lastname: Liu
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008168:Lung / Q000098:blood supply
chemical_list: C573420:ICAM1 protein, human; D018799:Intercellular Adhesion Molecule-1; D017228:Hepatocyte Growth Factor