title: [Effect of gambogic acid on proliferation of SKM-1 cells and its mechanism].
abstract: The aim of this study was to explore the effect of gambogic acid (GA) on MDS SKM-1 cell proliferation, apoptosis and their possible mechanism. Cell proliferation was determined by MTT method. The apoptosis percentage and cell cycle regulation of SKM-1 cells were analyzed by flow cytometry. Morphological features were observed by light microscopy. The mRNA expression of bcl-2 and bax were detected by RT-PCR. The results showed that GA could inhibit the proliferation of SKM-1 cells in a dose- and time-dependent manner (IC50 was 0.37 µg/ml at 48 h), increase the apoptotic percentage of SKM-1 cells, and arrest cell cycle at the G0/G1. The expression of bax mRNA was up-regulated while that of bcl-2 mRNA was down-regulated in SKM-1 cells treated with GA for 48 h. It is concluded that GA can induce apoptosis, which may be related to its effect of arresting cells at phase of G0/G1 and down-regulating bcl-2/bax ratio.
authors.affiliation: Department of Hematology, Southeast University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Yun-Yu
authors.initials: YY
authors.lastname: Sun
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Wang
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Xia
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Ding
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Song
authors.forename: Qing-Long
authors.initials: QL
authors.lastname: Guo
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Zhang
authors.forename: Chun-Rui
authors.initials: CR
authors.lastname: Li
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D044004:Xanthones / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D044004:Xanthones; D051028:bcl-2-Associated X Protein; C052659:gambogic acid