title: Descriptive morphometry and stereology of the tubular compartment in the wild rodent Hylaeamys megacephalus (Rodentia: Cricetidae) from Central Brazil.
abstract: Information on reproductive characteristics of wild rodents is scarce in the literature. This study aimed to assess the testis morphometry and stereology of Hylaeamys megacephalus. We used five animals in the study, captured in forest fragments in southwestern Goias State, between April and August 2009. The testes were fixed in Karnovsky solution, dehydrated, and embedded in methacrylate. Two-micrometer-thick sections from each sample were stained with toluidine blue/sodium borate 1%. Images of the testicular parenchyma were obtained from photomicroscope and morphometric and stereological analyses were carried out using the Image Pro-Plus software. The average body weight observed in the specimens of H. megacephalus in the study was 47.84 g, of which, 0.40% is allocated to the gonads (GSI) and 0.36% to the seminiferous tubules (TSI). These parameters suggest promiscuous reproductive behavior, of the polyandrous type, favoring males with higher sperm production and consequently, larger testes. The volume density of the seminiferous tubules was 94.46%, which represented a volume of 0.18 mL. The volume density and volume of the interstitium were 5.54% and 0.011 mL, respectively. The diameter of the seminiferous tubules was 206.5 μm and the height of seminiferous epithelium was 71.27 μm. H. megacephalus presents 5.06 m of seminiferous tubules and an average of 27.96 m of seminiferous tubules per gram of testis. The mitotic and meiotic indexes showed losses of 85 and 42%, respectively and an overall loss of 90% over the full spermatogenic process. The number of Sertoli cells per testis and per gram of testis was 7.8×10(6) and 95.28×10(6), respectively. Most of the morphometric parameters evaluated in H. megacephalus in this study are within the range of values described for most mammals.
authors.affiliation: Biological Sciences, Federal University of Goiás, Campus Jataí, Jataí, Brazil. Electronic address: facrismelo@yahoo.com.br.
authors.forename: Fabiana Cristina Silveira Alves
authors.initials: FC
authors.lastname: de Melo
authors.affiliation: Biological Sciences, Federal University of Goiás, Campus Jataí, Jataí, Brazil. Electronic address: tati-bio3004@hotmail.com.
authors.forename: Tatiane Pires
authors.initials: TP
authors.lastname: de Sousa
authors.affiliation: Department of General Biology, Federal University of Viçosa, Viçosa, Brazil. Electronic address: kyvia_lugatti@yahoo.com.br.
authors.forename: Kyvia Lugate C
authors.initials: KL
authors.lastname: Costa
authors.affiliation: Department of General Biology, Federal University of Viçosa, Viçosa, Brazil. Electronic address: spermatta@yahoo.com.br.
authors.forename: Sérgio Luis P
authors.initials: SL
authors.lastname: da Matta
authors.affiliation: Biological Sciences, Federal University of Goiás, Campus Jataí, Jataí, Brazil. Electronic address: frmelo@carangola.br.
authors.forename: Fabiano Rodrigues
authors.initials: FR
authors.lastname: de Melo
authors.affiliation: Biological Sciences, Federal University of Goiás, Campus Jataí, Jataí, Brazil. Electronic address: ricardo.santarita@pq.cnpq.br.
authors.forename: Ricardo de Mattos
authors.initials: Rde M
authors.lastname: Santa-Rita
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000033:anatomy & histology*; D001835:Body Weight / Q000502:physiology; D001938:Brazil; D006651:Histocytochemistry / Q000662:veterinary; D008297:Male; D009929:Organ Size / Q000502:physiology; D013737:Testis / Q000033:anatomy & histology*