title: Effects of titanium surface anodization with CaP incorporation on human osteoblastic response.
abstract: In this study we investigated whether anodization with calcium phosphate (CaP) incorporation (Vulcano®) enhances growth factors' secretion, osteoblast-specific gene expression, and cell viability, when compared to acid etched surfaces (Porous®) and machined surfaces (Screw®) after 3 and 7days. Results showed significant cell viability for Porous and Vulcano at day 7, when compared with Screw (p=0.005). At the same time point, significant differences regarding runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and bone sialoprotein (BSP) expression were found for all surfaces (p<0.05), but with greater fold induction for Porous and Vulcano. The secretion of transforming growth factor β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2) was not significantly affected by surface treatment in any experimental time (p>0.05). Although no significant correlation was found for growth factors' secretion and Runx2 expression, a significant positive correlation between this gene and ALP/BSP expression showed that their strong association is independent on the type of surface. The incorporation of CaP affected the biological parameters evaluated similar to surfaces just acid etched. The results presented here support the observations that roughness also may play an important role in determining cell response.
authors.affiliation: Biomaterials and Cellular Biomimicry Laboratory, School of Dentistry, Federal University of Uberlândia, MG, Brazil.
authors.forename: Natássia Cristina Martins
authors.initials: NC
authors.lastname: Oliveira
authors.forename: Camilla Christian Gomes
authors.initials: CC
authors.lastname: Moura
authors.forename: Darceny
authors.initials: D
authors.lastname: Zanetta-Barbosa
authors.forename: Daniela Baccelli Silveira
authors.initials: DB
authors.lastname: Mendonça
authors.forename: Lyndon
authors.initials: L
authors.lastname: Cooper
authors.forename: Gustavo
authors.initials: G
authors.lastname: Mendonça
authors.forename: Paula
authors.initials: P
authors.lastname: Dechichi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001857:Bone Matrix / Q000187:drug effects / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002130:Calcium Phosphates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004566:Electrodes; D005333:Fetus / Q000166:cytology; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis
chemical_list: D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D050659:Core Binding Factor Alpha 1 Subunit; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; C020243:calcium phosphate; D014025:Titanium; D000469:Alkaline Phosphatase