title: Mitochondrial network genes in the skeletal muscle of amyotrophic lateral sclerosis patients.
abstract: Recent evidence suggested that muscle degeneration might lead and/or contribute to neurodegeneration, thus it possibly play a key role in the etiopathogenesis and progression of amyotrophic lateral sclerosis (ALS). To test this hypothesis, this study attempted to categorize functionally relevant genes within the genome-wide expression profile of human ALS skeletal muscle, using microarray technology and gene regulatory network analysis. The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls. The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals. In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism. Overall, the analytical approach used in this study offer the possibility to observe higher levels of correlation (i.e. common expression trends) among genes, whose function seems to be aberrantly activated during the progression of muscle atrophy.
authors.affiliation: Institute of Anatomy and Cell Biology, School of Medicine, Università Cattolica del Sacro Cuore, Rome, Italy. camilla.bernardini@rm.unicatt.it
authors.forename: Camilla
authors.initials: C
authors.lastname: Bernardini
authors.forename: Federica
authors.initials: F
authors.lastname: Censi
authors.forename: Wanda
authors.initials: W
authors.lastname: Lattanzi
authors.forename: Marta
authors.initials: M
authors.lastname: Barba
authors.forename: Giovanni
authors.initials: G
authors.lastname: Calcagnini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giuliani
authors.forename: Giorgio
authors.initials: G
authors.lastname: Tasca
authors.forename: Mario
authors.initials: M
authors.lastname: Sabatelli
authors.forename: Enzo
authors.initials: E
authors.lastname: Ricci
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Michetti
mesh_terms: D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D053263:Gene Regulatory Networks / Q000235:genetics*; D050259:Genes, Mitochondrial / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D025341:Principal Component Analysis; D059467:Transcriptome