title: Technical note: proteomic approaches to fundamental questions about neutrophil biology.
abstract: Proteomics is one of a group of technologies that generates high-throughput, large-scale datasets that can be used to understand cell or organ functions at a systems level. This review will focus on the application of proteomics to the understanding of neutrophil biology. The strengths and weaknesses of common proteomic methods and their application to neutrophils are reviewed, with the goal of evaluating whether the technology is ready to advance our understanding of neutrophil biology.
authors.affiliation: 1.Baxter I Research Bldg., Rm. 102 South, 570 South Preston St., Louisville, KY 40202, USA. k.mcleish@louisville.edu.
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: McLeish
authors.forename: Michael L
authors.initials: ML
authors.lastname: Merchant
authors.forename: Jon B
authors.initials: JB
authors.lastname: Klein
authors.forename: Richard A
authors.initials: RA
authors.lastname: Ward
mesh_terms: D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012107:Research Design; D015398:Signal Transduction
chemical_list: D020543:Proteome
keywords: electrospray ionization; liquid chromatography; mass spectrometry