title: Rapid immunoassay method for the determination of clenbuterol and salbutamol in blood.
abstract: The aim of the study was to evaluate the adequacy of enzyme-linked immunosorbent assay (ELISA) in the post-exposure determination of the β-agonists clenbuterol and salbutamol in animal plasma and serum. Experimental guinea pigs (n = 20) were treated with two doses (0.25 and 2.5 mg/kg) of clenbuterol (n = 10) and salbutamol (n = 10) for seven days, whereas the control animal group (n = 10) was left untreated. Validation of the applied method yielded acceptable recovery (mean > 70%) and repeatability rates, showing ELISA to be applicable for the semi-quantitative determination of both analytes in both matrices, preferably in plasma. In both matrices, clenbuterol concentrations were proven to be significantly (14-fold) higher than those of salbutamol. Concentrations of both analytes were higher in plasma than in serum. The application of a 10-fold higher clenbuterol and salbutamol dose (2.5 mg/kg) resulted in concentrations 3- to 4-fold higher for clenbuterol and 2- to 3-fold higher for salbutamol, indicating a different release rate of these two β-agonists.
authors.affiliation: Laboratory for Analytical Chemistry, Croatian Veterinary Institute, 10000 Zagreb, Croatia. pleadin@veinst.hr
authors.forename: J
authors.initials: J
authors.lastname: Pleadin
authors.forename: A
authors.initials: A
authors.lastname: Vulić
authors.forename: N
authors.initials: N
authors.lastname: Perši
authors.forename: S
authors.initials: S
authors.lastname: Terzić
authors.forename: M
authors.initials: M
authors.lastname: Andrišić
authors.forename: I
authors.initials: I
authors.lastname: Žarković
mesh_terms: D000420:Albuterol / Q000097:blood*; D000818:Animals; D002976:Clenbuterol / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006168:Guinea Pigs; D008297:Male
chemical_list: D000420:Albuterol; D002976:Clenbuterol