title: Reversible redox regulation of specificity of Arg-gingipain B in Porphyromonas gingivalis.
abstract: Arg-gingipain B (RgpB), a major virulence factor secreted by the periodontal pathogen Porphyromonas gingivalis is an Arg-specific cysteine proteinase. By monitoring proteolytic cleavage of a human salivary peptide histatin 5 using MALDI-TOF MS, RgpB purified from P. gingivalis HG66 was found to shift from a dominant Arg-X to dominant Lys-X activity, both in vitro and in vivo, upon reversible cysteine oxidation. Native PAGE analysis revealed the association of novel Lys-X activity with a reversible state change of the oxidized enzyme. The redox-regulated Lys-X activity of RgpB may provide a survival advantage to P. gingivalis against the oxidative host defence.
authors.affiliation: Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.
authors.forename: Yu-Yen
authors.initials: YY
authors.lastname: Chen
authors.forename: Christine A
authors.initials: CA
authors.lastname: Seers
authors.forename: Nada
authors.initials: N
authors.lastname: Slakeski
authors.forename: Caroline
authors.initials: C
authors.lastname: Moore
authors.forename: Lianyi
authors.initials: L
authors.lastname: Zhang
authors.forename: Eric C
authors.initials: EC
authors.lastname: Reynolds
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D000080867:Gingipain Cysteine Endopeptidases; D054808:Histatins / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D016966:Porphyromonas gingivalis / Q000166:cytology / Q000201:enzymology*; D012995:Solubility; D013379:Substrate Specificity
chemical_list: D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; C068075:HTN3 protein, human; D054808:Histatins; D003546:Cysteine Endopeptidases