title: Structural features of a picornavirus polymerase involved in the polyadenylation of viral RNA.
abstract: Picornaviruses have 3' polyadenylated RNA genomes, but the mechanisms by which these genomes are polyadenylated during viral replication remain obscure. Based on prior studies, we proposed a model wherein the poliovirus RNA-dependent RNA polymerase (3D(pol)) uses a reiterative transcription mechanism while replicating the poly(A) and poly(U) portions of viral RNA templates. To further test this model, we examined whether mutations in 3D(pol) influenced the polyadenylation of virion RNA. We identified nine alanine substitution mutations in 3D(pol) that resulted in shorter or longer 3' poly(A) tails in virion RNA. These mutations could disrupt structural features of 3D(pol) required for the recruitment of a cellular poly(A) polymerase; however, the structural orientation of these residues suggests a direct role of 3D(pol) in the polyadenylation of RNA genomes. Reaction mixtures containing purified 3D(pol) and a template RNA with a defined poly(U) sequence provided data consistent with a template-dependent reiterative transcription mechanism for polyadenylation. The phylogenetically conserved structural features of 3D(pol) involved in the polyadenylation of virion RNA include a thumb domain alpha helix that is positioned in the minor groove of the double-stranded RNA product and lysine and arginine residues that interact with the phosphates of both the RNA template and product strands.
authors.affiliation: Department of Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Kempf
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Kelly
authors.forename: Courtney L
authors.initials: CL
authors.lastname: Springer
authors.forename: Olve B
authors.initials: OB
authors.lastname: Peersen
authors.forename: David J
authors.initials: DJ
authors.lastname: Barton
mesh_terms: D019943:Amino Acid Substitution; D004252:DNA Mutational Analysis; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D017955:Poliovirus / Q000201:enzymology* / Q000235:genetics; D026723:Polyadenylation; D011487:Protein Conformation; D012367:RNA, Viral / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; C090443:polymerase 3Dpol, poliovirus; D012324:RNA-Dependent RNA Polymerase