title: Histologic analysis of a novel extracellullar matrix membrane for guided bone regeneration: an experimental study in rabbits.
abstract: This experiment was conducted to study the histologic feasibility and biologic impact of Ti02 impregnation of an extracellullar matrix (ECM) membrane in guided bone regeneration. Eighteen adult New Zealand White rabbits were used. Bilateral bone defects were created in edentulous areas of the maxilla. The defects were filled with demineralized freeze-dried bone (DFDB). ECM was randomly pretreated with a suspension containing saline and 3 mg Ti02 granules. A regular ECM membrane served as a control on the contralateral side. Healing periods were 2, 4, and 8 weeks. Histologic and histomorphometric analyses were performed. The parameters assessed were (1) zone of inflammatory cells adjacent to ECM membrane, (2) presence of cellullar ingrowth into ECM, and (3) presence of Ti02 particles within the ECM barrier membrane. In general, no adverse reactions toward both groups of ECM membranes could be noted. The Ti02 particles remained within the ECM after 8 weeks of healing, making histologic detection of ECM easy. Histomorphometric analysis revealed low numbers of inflammatory cells adjacent to the ECM surface and adequate preservation and integration of the barrier. Contrasting Ti02 particles impregnated into the ECM membrane can be a very useful tool for the detection of similar biologic materials in in vivo models.
authors.affiliation: Department of Surgical Sciences, Kuwait University, Kuwait City, Kuwait.
authors.forename: Adel
authors.initials: A
authors.lastname: Al-Asfour
authors.forename: Pentti
authors.initials: P
authors.lastname: Tengvall
authors.forename: Lars
authors.initials: L
authors.lastname: Andersson
authors.forename: Christer
authors.initials: C
authors.lastname: Dahlin
mesh_terms: D016301:Alveolar Bone Loss / Q000601:surgery*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001857:Bone Matrix / Q000473:pathology; D001861:Bone Regeneration / Q000502:physiology; D016723:Bone Remodeling / Q000502:physiology; D016025:Bone Transplantation / Q000379:methods; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000737:chemistry; D005240:Feasibility Studies; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008437:Maxilla / Q000601:surgery; D018919:Neovascularization, Physiologic / Q000502:physiology; D010006:Osteoblasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D011817:Rabbits; D011897:Random Allocation; D012965:Sodium Chloride / Q000737:chemistry; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry; D014945:Wound Healing / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; C009495:titanium dioxide; D012965:Sodium Chloride; D014025:Titanium