title: Enhanced anti-cancer activity of human dendritic cells sensitized with gamma-irradiation-induced apoptotic colon cancer cells.
abstract: Properly sensitized dendritic cells (DCs) can be an effective immunotherapeutic against cancers. We investigated the phenotypic and functional changes in human DCs sensitized with γ-irradiated colon cancer cell-line HT-29 (GIH). GIH induced maturation and activation of DCs. GIH-sensitized DCs showed increased cytotoxic activity against HT-29 through higher expression of perforin and granzyme B. They further induced expression of effector cytokines, cytotoxic molecules, and mucosal-homing receptor in autologous T-cells. Conclusively, these results suggest that effective anti-cancer activity is induced when DCs are sensitized with γ-irradiated cancer cells via both direct augmentation of the cytotoxicity and indirect activation of T cells.
authors.affiliation: Department of Oral Microbiology and Immunology, DRI, and BK21 Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sun Kyung
authors.initials: SK
authors.lastname: Kim
authors.forename: Cheol-Heui
authors.initials: CH
authors.lastname: Yun
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Han
mesh_terms: D017209:Apoptosis; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D005720:Gamma Rays; D053804:Granzymes / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007167:Immunotherapy; D008213:Lymphocyte Activation; D054353:Perforin / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D016207:Cytokines; D016201:Receptors, Lymphocyte Homing; D054353:Perforin; D053804:Granzymes