title: Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor.
abstract: The selective detection of ultratrace amounts of aflatoxin M1 (AFM1) is extremely important for food safety since it is the most toxic mycotoxin class that is allowed to be present on cow milk with strictly low regulatory levels. In this work, Fe3O4 incorporated polyaniline (Fe3O4/PANi) film has been polymerized on interdigitated electrode (IDE) as sensitive film for AFM1 electrochemical biosensor. The immobilized aptamers as an affinity capture reagent and magnetic nanoparticles for signal amplification element have been employed in the sensing platform. Label-free and direct detection of the aptamer-AFM1 on Fe3O4/PANi interface were performed via electrochemical signal change, acquired by cyclic and square wave voltammetries. With a simplified strategy, this electrochemical aptasensor shows a good sensitivity to AFM1 in the range of 6-60 ng·L(-1), with the detection limit of 1.98 ng·L(-1). The results open up the path for designing cost effective aptasensors for other biomedical applications.
authors.affiliation: Institute of Material Science, Vietnam Academy of Science and Technology, 18, Hoang Quoc Viet Road, Cau Giay, Hanoi, Viet Nam.
authors.forename: Binh Hai
authors.initials: BH
authors.lastname: Nguyen
authors.forename: Lam Dai
authors.initials: LD
authors.lastname: Tran
authors.forename: Quan Phuc
authors.initials: QP
authors.lastname: Do
authors.forename: Huy Le
authors.initials: HL
authors.lastname: Nguyen
authors.forename: Ngoc Huan
authors.initials: NH
authors.lastname: Tran
authors.forename: Phuc Xuan
authors.initials: PX
authors.lastname: Nguyen
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis* / Q000737:chemistry; D000814:Aniline Compounds / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D015203:Reproducibility of Results; D013194:Staining and Labeling
chemical_list: D000814:Aniline Compounds; D052157:Aptamers, Nucleotide; C416807:polyaniline; D016607:Aflatoxin M1; D052203:Ferrosoferric Oxide