title: High temporal resolution parametric MRI monitoring of the initial ischemia/reperfusion phase in experimental acute kidney injury.
abstract: Ischemia/reperfusion (I/R) injury, a consequence of kidney hypoperfusion or temporary interruption of blood flow is a common cause of acute kidney injury (AKI). There is an unmet need to better understand the mechanisms operative during the initial phase of ischemic AKI. Non-invasive in vivo parametric magnetic resonance imaging (MRI) may elucidate spatio-temporal pathophysiological changes in the kidney by monitoring the MR relaxation parameters T2* and T2, which are known to be sensitive to blood oxygenation. The aim of our study was to establish the technical feasibility of fast continuous T2*/T2 mapping throughout renal I/R. MRI was combined with a remotely controlled I/R model and a segmentation model based semi-automated quantitative analysis. This technique enabled the detailed assessment of in vivo changes in all kidney regions during ischemia and early reperfusion. Significant changes in T2* and T2 were observed shortly after induction of renal ischemia and during the initial reperfusion phase. Our study demonstrated for the first time that continuous and high temporal resolution parametric MRI is feasible for in-vivo monitoring and characterization of I/R induced AKI in rats. This technique may help in the identification of the timeline of key events responsible for development of renal damage in hypoperfusion-induced AKI.
authors.affiliation: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany. andreas.pohlmann@mdc-berlin.de
authors.forename: Andreas
authors.initials: A
authors.lastname: Pohlmann
authors.forename: Jan
authors.initials: J
authors.lastname: Hentschel
authors.forename: Mandy
authors.initials: M
authors.lastname: Fechner
authors.forename: Uwe
authors.initials: U
authors.lastname: Hoff
authors.forename: Gordana
authors.initials: G
authors.lastname: Bubalo
authors.forename: Karen
authors.initials: K
authors.lastname: Arakelyan
authors.forename: Kathleen
authors.initials: K
authors.lastname: Cantow
authors.forename: Erdmann
authors.initials: E
authors.lastname: Seeliger
authors.forename: Bert
authors.initials: B
authors.lastname: Flemming
authors.forename: Helmar
authors.initials: H
authors.lastname: Waiczies
authors.forename: Sonia
authors.initials: S
authors.lastname: Waiczies
authors.forename: Wolf-Hagen
authors.initials: WH
authors.lastname: Schunck
authors.forename: Duska
authors.initials: D
authors.lastname: Dragun
authors.forename: Thoralf
authors.initials: T
authors.lastname: Niendorf
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D015427:Reperfusion Injury / Q000473:pathology*