title: A novel ribonuclease with HIV-1 reverse transcriptase inhibitory activity from the edible mushroom Hygrophorus russula.
abstract: A 28-kDa ribonuclease, with an optimum pH of 4.0 and an optimum temperature at 58 °C, was isolated from fruiting bodies of the edible mushroom Hygrophorus russula. It was purified by ion exchange chromatography on carboxymethyl-cellulose, dithyaminoethyl-cellulose, quaternary amine-sepharose and sulphopropyl-sepharose, followed by fast protein liquid chromatography gel filtration on Superdex 75. The N-terminal amino acid sequence was ASAGG which showed homology to those of other fungal RNases to some degree. It exerted the highest RNase activity on poly C and poly U. The Michaelis constant (K(m)) value of the RNase on yeast tRNA was 3.6 μM, and the maximal velocity (V(max)) was 0.6 μM. The RNase activity was suppressed by some ions including Fe(2+) and Zn(2+) ions. The RNase inhibited the activity of HIV-1 reverse transcriptase with an IC(50) of 4.64 μM.
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.
authors.forename: Mengjuan
authors.initials: M
authors.lastname: Zhu
authors.forename: Lijing
authors.initials: L
authors.lastname: Xu
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Zengqiang
authors.initials: Z
authors.lastname: Ma
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000201:enzymology; D019380:Anti-HIV Agents / Q000737:chemistry* / Q000302:isolation & purification; D002413:Cations, Divalent; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D011066:Poly C / Q000737:chemistry; D011072:Poly U / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry* / Q000302:isolation & purification; D013696:Temperature; D015032:Zinc / Q000737:chemistry
chemical_list: D019380:Anti-HIV Agents; D002413:Cations, Divalent; D005656:Fungal Proteins; D011072:Poly U; D011066:Poly C; D007501:Iron; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases; D015032:Zinc