title: Experimental infection with a Thai reassortant swine influenza virus of pandemic H1N1 origin induced disease.
abstract: BACKGROUND
  Following the emergence of the pandemic H1N1 influenza A virus in 2009 in humans, this novel virus spread into the swine population. Pigs represent a potential host for this virus and can serve as a mixing vessel for genetic mutations of the influenza virus. Reassortant viruses eventually emerged from the 2009 pandemic and were reported in swine populations worldwide including Thailand. As a result of the discovery of this emergent disease, pathogenesis studies of this novel virus were conducted in order that future disease protection and control measures in swine and human populations could be enacted.
  
  
  METHODS
  The pandemic H1N1 2009 virus (pH1N1) and its reassortant virus (rH1N1) isolated from pigs in Thailand were inoculated into 2 separate cohorts of 9, 3-week-old pigs. Cohorts were consisted of one group experimentally infected with pH1N1 and one group with rH1N1. A negative control group consisting of 3 pigs was also included. Clinical signs, viral shedding and pathological lesions were investigated and compared. Later, 3 pigs from viral inoculated groups and 1 pig from the control group were necropsied at 2, 4, and 12 days post inoculation (DPI).
  
  
  RESULTS
  The results indicated that pigs infected with both viruses demonstrated typical flu-like clinical signs and histopathological lesions of varying severity. Influenza infected-pigs of both groups had mild to moderate pulmonary signs on 1-4 DPI. Interestingly, pigs in both groups demonstrated viral RNA detection in the nasal swabs until the end of the experiment (12 DPI).
  
  
  CONCLUSIONS
  The present study demonstrated that both the pH1N1 and rH1N1 influenza viruses, isolated from naturally infected pigs, induced acute respiratory disease in experimentally inoculated nursery pigs. Although animals in the rH1N1-infected cohort demonstrated more severe clinical signs, had higher numbers of pigs shedding the virus, were noted to have increased histopathological severity of lung lesions and increased viral antigen in lung tissue, the findings were not statistically significant in comparison with the pH1N1-infected group. Interestingly, viral genetic material of both viruses could be detected from the nasal swabs until the end of the experiment. Similar to other swine influenza viruses, the clinical signs and pathological lesions in both rH1N1 and pH1N1 were limited to the respiratory tract.
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd, Bangkok 10330, Thailand.
authors.forename: Nataya
authors.initials: N
authors.lastname: Charoenvisal
authors.forename: Juthatip
authors.initials: J
authors.lastname: Keawcharoen
authors.forename: Donruethai
authors.initials: D
authors.lastname: Sreta
authors.forename: Siriporn
authors.initials: S
authors.lastname: Tantawet
authors.forename: Suphattra
authors.initials: S
authors.lastname: Jittimanee
authors.forename: Jirapat
authors.initials: J
authors.lastname: Arunorat
authors.forename: Alongkorn
authors.initials: A
authors.lastname: Amonsin
authors.forename: Roongroje
authors.initials: R
authors.lastname: Thanawongnuwech
mesh_terms: D000818:Animals; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D058873:Pandemics; D016865:Reassortant Viruses / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000821:virology*; D013785:Thailand / Q000453:epidemiology; D014774:Virulence