title: Enhanced fluorescent assignment of protein aggregates by an oligothiophene-porphyrin-based amyloid ligand.
abstract: Fluorescent probes identifying protein aggregates are of great interest, as deposition of aggregated proteins is associated with many devastating diseases. Here, we report that a fluorescent amyloid ligand composed of two distinct molecular moieties, an amyloidophilic pentameric oligothiophene and a porphyrin, can be utilized for spectral and lifetime imaging assessment of recombinant Aβ 1-42 amyloid fibrils and Aβ deposits in brain tissue sections from a transgenic mouse model with Alzheimer's disease pathology. The enhanced spectral range and distinct lifetime diversity of this novel oligothiophene-porphyrin-based ligand allow a more precise assessment of heterogeneous amyloid morphology compared with the corresponding oligothiophene dye.
authors.affiliation: Department of Chemistry, Linköping University, SE-581 83 Linköping, Sweden.
authors.forename: Katriann
authors.initials: K
authors.lastname: Arja
authors.forename: Daniel
authors.initials: D
authors.lastname: Sjölander
authors.forename: Alma
authors.initials: A
authors.lastname: Åslund
authors.forename: Stefan
authors.initials: S
authors.lastname: Prokop
authors.forename: Frank L
authors.initials: FL
authors.lastname: Heppner
authors.forename: Peter
authors.initials: P
authors.lastname: Konradsson
authors.forename: Mikael
authors.initials: M
authors.lastname: Lindgren
authors.forename: Per
authors.initials: P
authors.lastname: Hammarström
authors.forename: K O Andreas
authors.initials: KO
authors.lastname: Åslund
authors.forename: K Peter R
authors.initials: KP
authors.lastname: Nilsson
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011166:Porphyrins / Q000737:chemistry*; D055503:Protein Multimerization; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes; D008024:Ligands; D010446:Peptide Fragments; D011166:Porphyrins; D013876:Thiophenes; C075222:amyloid beta-protein (1-42)