title: Effect of an isotonic lubricant on sperm collection and sperm quality.
abstract: OBJECTIVE
  To assess the influence of an isotonic lubricant used during sperm sample collection on [1] ease of collection and [2] resultant sperm quality.
  
  
  METHODS
  Paired randomized cross-over design.
  
  
  METHODS
  Tertiary hospital.
  Healthy men over 18 years old with normal semen analysis as per World Health Organization 2010 guidelines.
  
  
  METHODS
  Collection of semen sample from 22 subjects by masturbation with or without the use of Pre-Seed personal lubricant.
  
  
  METHODS
  Qualitative survey results and quantitative sperm function outcomes were measured to determine resultant sperm quality and collection experience with and without Pre-Seed lubricant.
  
  
  RESULTS
  The qualitative questionnaire results showed that 73% of donors prefer the semen collection process with the isotonic lubricant and 55% recommended the use of lubricant in their everyday collection. The motility, viability, membrane integrity, levels of reactive oxygen species, total antioxidant capacity, and percentage of DNA damage in collected semen samples were not affected by the use of the lubricant.
  
  
  CONCLUSIONS
  More donors prefer, and find it easier, to collect semen samples with the use of the lubricant. The isotonic lubricant Pre-Seed did not compromise sperm quality as evaluated in an array of sperm assays, suggesting its safe use in fertility patients as required during sperm collection.
authors.affiliation: Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio, USA. Agarwaa@ccf.org
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
authors.forename: Helena
authors.initials: H
authors.lastname: Malvezzi
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sharma
mesh_terms: D018592:Cross-Over Studies; D006801:Humans; D007552:Isotonic Solutions; D054327:Lubricants / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D008418:Masturbation; D055101:Semen Analysis / Q000379:methods; D013076:Sperm Count / Q000379:methods; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology*
chemical_list: D007552:Isotonic Solutions; D054327:Lubricants