title: Graphene oxide as a nanocarrier for loading and delivery of medicinal drugs and as a biosensor for detection of serum albumin.
abstract: The interaction of graphene oxide (GO), a medicinal drug (10-hydroxy camptothecin (HCPT)), and bovine serum albumin (BSA) was investigated with the aim of developing a method for the analysis of serum albumin proteins. It was demonstrated that HCPT could be readily loaded onto GO via the π-π stacking interaction, and the delivery of HCPT to BSA was improved in the presence of GO; this, in turn, facilitated the binding interaction of HCPT and BSA. Chemometrics methods, multivariate curve resolution-alternating least squares (MCR-ALS) and parallel factor analysis (PARAFAC), were applied to resolve spectral data, and this assisted in the elucidation of the above interaction. GO was found to enhance the fluorescence response of HCPT to BSA, and thus, a low cost fluorescence bio-sensing platform was developed for fluorescence-enhanced detection of BSA based on GO. The satisfactory analytical performance of this biosensor for BSA was attributed to the structure and electronic properties of GO.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. ynni@ncu.edu.cn
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Ni
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zhang
authors.forename: Serge
authors.initials: S
authors.lastname: Kokot
mesh_terms: D015374:Biosensing Techniques; D002166:Camptothecin / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D006108:Graphite / Q000737:chemistry*; D016018:Least-Squares Analysis; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000032:analysis*
chemical_list: D004337:Drug Carriers; D010087:Oxides; D012710:Serum Albumin, Bovine; D006108:Graphite; D002166:Camptothecin