title: Effects of PDGF-BB and OP-1 on mesenchymal stem cells in a porous mineral block.
abstract: The aim of this study was to evaluate the effects of an osteogenic medium supplemented with platelet-derived growth factor (PDGF) BB and osteogenic protein (OP) 1 on the proliferation and differentiation of mesenchymal stem cells (MSCs) in an anorganic bovine cancellous bone scaffold. At day 7, there was a statistically significant increase in the number of cells in the scaffolds in the group treated with the medium supplemented with both PDGF-BB and OP-1 when compared with the control groups. The highest alkaline phosphate levels, at 14 and 21 days, were recorded for the samples in medium supplemented with OP-1 alone reflecting osteogenic differentiation. The results commend OP-1, as well as PDGF-BB, for incorporation into porous mineral scaffolds for vertical ridge augmentation.
authors.affiliation: Tissue Engineering, VA Boston Healthcare System, Boston, Massachusetts 02130, USA.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Wada
authors.forename: Victor H
authors.initials: VH
authors.lastname: Gerbaudo
authors.forename: Myron
authors.initials: M
authors.lastname: Spector
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D000077214:Becaplermin; D001857:Bone Matrix / Q000737:chemistry; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D004247:DNA / Q000032:analysis; D005260:Female; D006041:Goats; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D013669:Technetium Tc 99m Medronate; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D043925:Angiogenesis Inducing Agents; C525819:BMP7 protein, human; C077540:Bio-Oss; D055419:Bone Morphogenetic Protein 7; D003470:Culture Media; D008903:Minerals; D020574:Proto-Oncogene Proteins c-sis; D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D000077214:Becaplermin; D004247:DNA; D000469:Alkaline Phosphatase; D013669:Technetium Tc 99m Medronate