title: [Verification of multipotential mesenchymal stem cell presence in peripheral blood of rabbits].
abstract: The aim of this study was to verify the presence of multipotential mesenchymal stem cells in peripheral blood (PBMSC) of rabbits. For mobilization, granulocyte-colony-stimulating factor 30 µg/(kg·d) was injected into New Zealand White rabbits subcutaneously for 6 d, then the PBMSC were isolated from peripheral blood of rabbits by density gradient centrifugation and adhesive culture. The morphology of cell proliferation was observed by microscopy, the proliferative curve of cells was drawn. The phenotypes of PBMSC were detected by flow cytometry, the differential capability of PBMSC into osteocytes, chondrocytes and adipocytes was identified. The results showed that the morphology of subcultured PBMSC were spindle or polygonal shaped, and cell population doubling time was 37.4 h. The isolated PBMSC expressed mesenchymal marker CD29, but not expressed hematopoietic marker CD14. Under specific induction conditions, PBMSC demonstrated multipotency to differentiate into osteocytes, chondrocytes and adipocytes. It is concluded that PBMSC are successfully isolated from peripheral blood and cultured, and their multipotential capability of differentiation into osteocytes, chondrocytes and adipocytes are verified.
authors.affiliation: Zunyi Medical College Affiliated Hospital, Zunyi, Guizhou Province, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Fang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Huang
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Liu
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Tang
mesh_terms: D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology; D011817:Rabbits