title: The influence of surface nanoroughness of electrospun PLGA nanofibrous scaffold on nerve cell adhesion and proliferation.
abstract: Electrospun nanofibrous scaffolds in neural tissue engineering provide an alternative approach for neural regeneration. Since the topography of a surface affects the microscopic behaviour of material; the creation of nanoscale surface features, which mimic the natural roughness of live tissue, on polymer surfaces can promote an appropriate cell growth and proliferation. In this research, a unique PLGA nanofibrous structure was fabricated without any post-electrospinning treatment. Scaffolds were prepared in two general groups: cylindrical and ribbon-shaped electrospun fibres, with smooth and rough (porous and grooved) surfaces. The experiments about nerve cell culture have demonstrated that the nanoroughness of PLGA electrospun scaffolds can increase the cell growing rate to 50 % in comparison with smooth and conventional electrospun scaffolds. SEM and AFM images and MTT assay results have shown that the roughened cylindrical scaffolds enhance the nerve growth and proliferation compared to smooth and ribbon-shaped nanofibrous scaffolds. A linear interaction has been found between cell proliferation and surface features. This helps to approximate MTT assay results by roughness parameters.
authors.affiliation: Department of Textile Engineering, Textile Research and Excellence Centers, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Zamani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Amani-Tehran
authors.forename: Masoud
authors.initials: M
authors.lastname: Latifi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004563:Electrochemistry / Q000379:methods; D019544:Equipment Failure Analysis; D005910:Glioma / Q000473:pathology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure*; D009416:Nerve Regeneration / Q000502:physiology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011474:Prosthesis Design; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid