title: A novel surface plasmon resonance biosensor based on graphene oxide decorated with gold nanorod-antibody conjugates for determination of transferrin.
abstract: A surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) decorated with gold nanorod (AuNR)-antibody conjugates was developed. Compared with traditional SPR biosensor, GO sheets were assembled on the amino-modified Au film via electrostatic interaction, and then AuNR-antibody conjugates were immobilized via carbodiimide-assisted amidation reaction. The abundant oxygen-containing functional groups, large specific surface area and friendly biocompatibility of GO sheets are beneficial to the immobilization of AuNR-antibody conjugates. Meanwhile, AuNRs are anchored to the GO surface through antibody and function as enhancers for the transferrin determination, which greatly enhance the sensitivity of the detection. As a result, the present biosensor shows a satisfactory response to transferrin in the concentration range of 0.0375-40.00 μg mL(-1). The lowest concentration of transferrin that can be determined by this method is about 32-fold lower than that obtained with the sensor based on Au film. This design affords a facile method of controlling the assembly of AuNRs on the GO sheets and can be easily extended to other protein detection by preparing corresponding AuNR-antibody conjugates.
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Daqian
authors.initials: D
authors.lastname: Song
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry; D020349:Surface Plasmon Resonance; D014168:Transferrin / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D014168:Transferrin; D006046:Gold; D006108:Graphite