title: Structural characterisation of polysaccharides from Tricholoma matsutake and their antioxidant and antitumour activities.
abstract: In this study, polysaccharides from Tricholoma matsutake (TM-P) were purified using a DEAE-Sepharose fast flow column and three polysaccharide fractions (TM-P1, TM-P2 and TM-P3) were obtained. The chemical composition and structural characteristics of TM-P2 were quite different from those of TM-P1 and TM-P3. TM-P2 consisted of glucose, galactose and mannose with a molar ratio of 5.9:1.1:1.0. The glycosidic linkages were mainly composed of 1,6- and 1-linked glucose. Furthermore, TM-P2 showed the strongest in vitro antioxidant and antitumour activities. The oxygen radical absorbance capacity (ORAC) of TM-P2 was 2100.44 μmol Trolox/g. The antiproliferative activities of TM-P2 (4.0mg/ml) on the growth of HepG2 and A549 cells were 67.98% and 59.04%, respectively.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: You
authors.forename: Qing
authors.initials: Q
authors.lastname: Gao
authors.forename: Mengying
authors.initials: M
authors.lastname: Feng
authors.forename: Bao
authors.initials: B
authors.lastname: Yang
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
authors.forename: Longjian
authors.initials: L
authors.lastname: Gu
authors.forename: Chun
authors.initials: C
authors.lastname: Cui
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides