title: In situ synthesized novel biocompatible titania-chitosan nanocomposites with high surface area and antibacterial activity.
abstract: A series of titania-chitosan nanocomposites (2:x (0.12, 0.25, 0.5, 1.0 and 2.0g)) were synthesized using in situ sol-gel method and comprehensively characterized using conventional techniques. The resultant particles showed anatase phase, spherical and irregular morphology with particle size of 4.5-10.5nm. Nanocomposites with higher surface area (114-265m(2)/g) and high purity were obtained. The characterized samples were analyzed in 1.5mM simulated body fluid (1.5 SBF) and human gastric adenocarcinoma cell line to explore the bioactivity and biocompatibility. Antibacterial activity against Staphylococcus aureus was also evaluated. The formation of apatite layer on 1.5 SBF-immersed samples confirms the bioactivity of all the nanocomposites. High surface area, appropriate hydroxyapatite formation, specific antibacterial action, increased cell viability, controlled swelling and degrading rate are favorably achieved at 2:1 nanocomposite ratio. This study shows titania-chitosan nanocomposites as the promising biomaterial for orthopedic and tissue engineering applications.
authors.affiliation: Centre for Nano Science and Technology, K. S. Rangasamy College of Technology, K.S.R. Kalvi Nagar, Tiruchengode-637 215, Namakkal (Dt.), Tamil Nadu, India. kkavitha07@gmail.com
authors.forename: K
authors.initials: K
authors.lastname: Kavitha
authors.forename: S
authors.initials: S
authors.lastname: Sutha
authors.forename: M
authors.initials: M
authors.lastname: Prabhu
authors.forename: V
authors.initials: V
authors.lastname: Rajendran
authors.forename: T
authors.initials: T
authors.lastname: Jayakumar
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D013211:Staphylococcus aureus / Q000187:drug effects; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology; D018675:Toxicity Tests / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D048271:Chitosan; D014025:Titanium