title: ROR2 and Wnt5a expression in stage 1 pure testicular seminomas.
abstract: OBJECTIVE
  To elucidate the prognostic utility of several biomarkers including ROR2/Wnt5a in stage 1 pure seminoma, which is a highly curable tumor in need of prognostic markers to avoid unnecessary treatment.
  
  
  METHODS
  A total of 47 patients of stage 1 seminoma who underwent radical orchiectomy were included in the study. Tissue microarray-based immunohistochemical analysis of placental alkaline phosphatase, D2-40, c-Kit, Oct-3/4, ROR2, Wnt5a, beta-catenin, CD30, vimentin, pancytokeratin, beta-hCG and p53 was conducted, and relevant clinicopathologic features were assessed.
  
  
  RESULTS
  ROR2 protein revealed strong diffuse membranous immunoreactivity (IR) in 12.8% and partial weak IR in 40.4%, respectively. Cytoplasimc Wnt5a IR was observed in 27.7%. ROR2 IR was correlated with Wnt5a IR (p = 0.029) and Oct3/4 IR (p = 0.035). c-Kit IR was correlated with Wnt5a IR (p = 0.034). No significant differences were found between ROR2/Wnt5a protein expression and the prognostically relevant features such as lymphatic invasion or pathologic T stage. Pathologic T stage was not correlated with rete invasion (p= 0.23). The expression or loss of other aforementioned antibodies was not associated with the prognostic clinicopathologic characteristics.
  
  
  CONCLUSIONS
  Our results do not support the relevance of ROR2/Wnt5a as biomarkers in stage 1 pure seminomas. The utility of the explored biomarkers as prognostic or differentiation indicators remains to be clarified.
authors.affiliation: Department of Pathology, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Republic of Korea.
authors.forename: So Dug
authors.initials: SD
authors.lastname: Lim
authors.forename: Wan Seop
authors.initials: WS
authors.lastname: Kim
authors.forename: Ghee Young
authors.initials: GY
authors.lastname: Kwon
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis*; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000032:analysis / Q000096:biosynthesis*; D012189:Retrospective Studies; D018239:Seminoma / Q000378:metabolism* / Q000473:pathology; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology; D046888:Tissue Array Analysis; D051153:Wnt Proteins / Q000032:analysis / Q000096:biosynthesis*; D000071818:Wnt-5a Protein; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C078717:ROR2 protein, human; D057050:Receptor Tyrosine Kinase-like Orphan Receptors