title: Characterization and DNA binding studies of unexplored imidazolidines by electronic absorption spectroscopy and cyclic voltammetry.
abstract: UV-Vis spectroscopic behavior of four imidazolidine derivatives i.e., [5-benzylideneimidazolidine-2,4-dione (NBI), 5-(2-hydroxybenzylidene)imidazolidine-2,4-dione (HBI), 5-(4-methoxybenzylidene)imidazolidine-2,4-dione (MBI) and 5-(3,4-di-methoxybenzylidene)imidazolidine-2,4-dione (DBI)] was studied in a wide pH range. Spectroscopic response of the studied compounds was found sensitive to pH and the attached substituents. Incited by anti-tumor activity, structural miscellany and biological applications of imidazolidines, the DNA binding affinity of some novel derivatives of this class of compounds was examined by cyclic voltammetry (CV) and UV-Vis spectroscopy at pH values of blood (7.4) and lysosomes (4.5). The CV results showed the following order of binding strength: KNBI (6.40×10(6)M(-1))>KHBI (1.77×10(5)M(-1))>KMBI (2.06×10(4)M(-1))>KDBI (1.01×10(4)M(-1)) at pH 7.4. The same order was also obtained from UV-Vis spectroscopy. The greater affinity of NBI justified its preferred candidature as an effective anti-cancer drug. The DNA binding propensity of these compounds was found comparable or greater than most of the clinically used anticancer drugs.
authors.affiliation: Department of Chemistry, Quaid-i-Azam University, 45320 Islamabad, Pakistan. afzals_qau@yahoo.com
authors.forename: Afzal
authors.initials: A
authors.lastname: Shah
authors.forename: Erum
authors.initials: E
authors.lastname: Nosheen
authors.forename: Shamsa
authors.initials: S
authors.lastname: Munir
authors.forename: Amin
authors.initials: A
authors.lastname: Badshah
authors.forename: Rumana
authors.initials: R
authors.lastname: Qureshi
authors.forename: Zia-Ur-
authors.initials: ZU
authors.lastname: Rehman
authors.forename: Niaz
authors.initials: N
authors.lastname: Muhammad
authors.forename: Hidayat
authors.initials: H
authors.lastname: Hussain
mesh_terms: D000042:Absorption; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D015195:Drug Design; D004563:Electrochemistry; D004583:Electrons; D006863:Hydrogen-Ion Concentration; D048289:Imidazolidines / Q000737:chemistry* / Q000378:metabolism*; D013057:Spectrum Analysis
chemical_list: D000970:Antineoplastic Agents; D048289:Imidazolidines; D004247:DNA