title: Identification and verification of microRNAs by high-throughput sequencing.
abstract: High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.
authors.affiliation: School of Biotechnology, Division of Gene Technology, Science for Life Laboratory (SciLifeLab Stockholm), KTH Royal Institute of Technology, Solna, Sweden.
authors.forename: Jimmie
authors.initials: J
authors.lastname: Hällman
authors.forename: Lotta
authors.initials: L
authors.lastname: Avesson
authors.forename: Johan
authors.initials: J
authors.lastname: Reimegård
authors.forename: Max
authors.initials: M
authors.lastname: Käller
authors.forename: Fredrik
authors.initials: F
authors.lastname: Söderbom
mesh_terms: D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D019295:Computational Biology; D004023:Dictyostelium / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D016053:RNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D035683:MicroRNAs; D016053:RNA, Protozoan