title: On the efficacy of malaria DNA vaccination with magnetic gene vectors.
abstract: We investigated the efficacy and types of immune responses from plasmid malaria DNA vaccine encoding VR1020-PyMSP119 condensed on the surface of polyethyleneimine (PEI)-coated SPIONs. In vivo mouse studies were done firstly to determine the optimum magnetic vector composition, and then to observe immune responses elicited when magnetic vectors were introduced via different administration routes. Higher serum antibody titers against PyMSP119 were observed with intraperitoneal and intramuscular injections than subcutaneous and intradermal injections. Robust IgG2a and IgG1 responses were observed for intraperitoneal administration, which could be due to the physiology of peritoneum as a major reservoir of macrophages and dendritic cells. Heterologous DNA prime followed by single protein boost vaccination regime also enhanced IgG2a, IgG1, and IgG2b responses, indicating the induction of appropriate memory immunity that can be elicited by protein on recall. These outcomes support the possibility to design superparamagnetic nanoparticle-based DNA vaccines to optimally evoke desired antibody responses, useful for a variety of diseases including malaria.
authors.affiliation: Department of Chemical Engineering, Monash University, Clayton VIC 3800, Australia.
authors.forename: Fatin
authors.initials: F
authors.lastname: Nawwab Al-Deen
authors.forename: Charles
authors.initials: C
authors.lastname: Ma
authors.forename: Sue D
authors.initials: SD
authors.lastname: Xiang
authors.forename: Cordelia
authors.initials: C
authors.lastname: Selomulya
authors.forename: Magdalena
authors.initials: M
authors.lastname: Plebanski
authors.forename: Ross L
authors.initials: RL
authors.lastname: Coppel
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D005822:Genetic Vectors; D007074:Immunoglobulin G / Q000097:blood; D060328:Magnetic Phenomena; D017780:Malaria Vaccines / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000913:Antibodies, Protozoan; D005290:Ferric Compounds; D007074:Immunoglobulin G; D017780:Malaria Vaccines; D019444:Vaccines, DNA; C000499:ferric oxide; D011094:Polyethyleneimine; D004247:DNA