title: Poly (ε-caprolactone) scaffolds functionalized by grafting NGF and GRGD promote growth and differentiation of PC12 cells.
abstract: Poly(ε-caprolactone) (PCL) scaffolds functionalized by grafting nerve growth factor (NGF) and Asp-Arg-Gly-Asp (GRGD)(PCL-NGF/GRGD) for neural tissue engineering. The influences of PCL-NGF/GRGD scaffolds on the growth and differentiation of PC12 cells were investigated. The successfully grafting NGF and GRGD into PCL-CS scaffold were verified by FTIR spectra. The densities of GRGD and NGF in the scaffolds were about 2.10×10(-1) μmol/cm(2) and 1.51×10(-3) nmol/cm(2) . Growths of PC12 cells in PCL-GRGD and PCL/NGF-GRGD scaffolds via MTS measurements were significantly higher (p < 0.01, n = 4) than that in PCL-CS or PCL-NGF ones for three days of cultivation that was consistent with SEM observations. Moreover, the differentiation of PC12 cells, induced by NGF at 50 ng/mL for four days, in PCL-NGF/GRGD scaffolds were qualitatively more numbers and longer outgrowth of neurites than those in PCL-CS, PCL-GRGD, and PCL-NGF ones by SEM observations. The synergistic effects of grafting both NGF and GRGD ligands to PCL-CS scaffolds on the growth and differentiation of PC12 cells provide a new biomaterial for neural tissue engineering.
authors.affiliation: Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Dou-Liu, Yun-Lin, 640 Taiwan, ROC.
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.forename: Dar-Ming
authors.initials: DM
authors.lastname: Lai
authors.forename: Shin-Der
authors.initials: SD
authors.lastname: Chen
authors.forename: Ya-I
authors.initials: YI
authors.lastname: Lin
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D055767:Immobilized Proteins / Q000737:chemistry*; D008422:Materials Testing; D020932:Nerve Growth Factor / Q000737:chemistry*; D009417:Nerve Tissue / Q000166:cytology / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry*; D016716:PC12 Cells; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D009842:Oligopeptides; D011091:Polyesters; C016240:polycaprolactone; D020932:Nerve Growth Factor; C064597:glycyl-arginyl-glycyl-aspartic acid
keywords: GRGD; NGF; PC12 cell; PCL scaffolds; neural tissue engineering