title: Characterization of phenolics of Sarcandra glabra by non-targeted high-performance liquid chromatography fingerprinting and following targeted electrospray ionisation tandem mass spectrometry/time-of-flight mass spectrometry analyses.
abstract: In this study, we successfully characterised the phenolic profiles of Sarcandra glabra (Thunb.) Nakai by high-performance liquid chromatography (HPLC) fingerprinting analyses and mass spectrometry (MS) identification. We first established a specific and valid HPLC approach for fingerprint analysis of S. glabra based on HPLC-UV detection. Using several chemometric methods such as similarity evaluation and principal components analysis, we determined herb-markers peaks from many HPLC peaks. The structures of these herb-markers were further identified targetedly by electrospray ionisation tandem mass spectrometry (ESI-MS/MS)/time-of-flight mass spectrometry (TOF-MS) analyses. As results, four phenolics, including chlorogenic acid, caffeic acid, 4-O-glucopyranosyl rosmarinic acid and rosmarinic acid, were characterised as major herb-markers for the stems of S. glabra, while another three phenolics, including kaempferol-3-O-β-d-glucuronic acid, chlorogenic acid and rosmarinic acid, were characteristic components for the leaves. The compounds may be very useful for further phenolome analysis.
authors.affiliation: Research and Development Center of Natural Medicine, Zhejiang Agriculture and Forestry University, Lin'an, Zhejiang Province 311300, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Junling
authors.initials: J
authors.lastname: Liang
authors.forename: Da
authors.initials: D
authors.lastname: Lv
authors.forename: Yinfei
authors.initials: Y
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jinping
authors.initials: J
authors.lastname: Si
authors.forename: Shihua
authors.initials: S
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010636:Phenols; D010936:Plant Extracts