title: Identification of respiratory viruses with a novel point-of-care multianalyte antigen detection test in children with acute respiratory tract infection.
abstract: BACKGROUND
  Rapid etiological diagnosis of a respiratory virus infection may have impact on antiviral and antibiotic therapy, patient cohorting, and prediction of the clinical course. Most point-of-care tests for detection of respiratory viruses have limitations in diagnostic performance and clinical usability. A novel, multianalyte point-of-care antigen detection test system (mariPOC(®); ArcDia International Oy Ltd., Turku, Finland) detects eight respiratory viruses (influenza A and B viruses, respiratory syncytial virus (RSV), adenovirus, human metapneumovirus, and parainfluenza type 1, 2, and 3 viruses) from a single nasopharyngeal swab specimen by a fully automated, random-access immunoassay method.
  
  
  OBJECTIVE
  To evaluate mariPOC(®) point-of-care test system in comparison with reverse transcription polymerase chain reaction (RT-PCR) in a pediatric emergency department setting.
  
  
  METHODS
  Prospectively collected samples from 158 children (mean age, 1.8 years) with respiratory symptoms and/or fever were analyzed both by mariPOC(®) and by multiplex RT-PCR.
  
  
  RESULTS
  The sensitivities and specificities (95% confidence intervals) of the mariPOC(®) test were for influenza A (n = 7), 71% (38-100) and 100%; influenza B (n = 22), 86% (72-100) and 98% (95-100); RSV (n = 35), 89% (78-99) and 100%; adenovirus (n = 12), 25% (1-50) and 97% (95-99); and for human metapneumovirus (n = 8), 50% (15-85) and 100%, respectively. Parainfluenzaviruses were detected only in five patients.
  
  
  CONCLUSIONS
  This novel point-of-care test system is a rapid, practical, and specific method for simultaneous detection of eight respiratory viruses. Compared with RT-PCR, its sensitivity is moderately high for detection of RSV and influenza viruses, and low for adenovirus.
authors.affiliation: Department of Pediatrics, Turku University Hospital, 20521 Turku, Finland.
authors.forename: Lauri
authors.initials: L
authors.lastname: Ivaska
authors.forename: Jussi
authors.initials: J
authors.lastname: Niemelä
authors.forename: Terho
authors.initials: T
authors.lastname: Heikkinen
authors.forename: Tytti
authors.initials: T
authors.lastname: Vuorinen
authors.forename: Ville
authors.initials: V
authors.lastname: Peltola
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007223:Infant; D007231:Infant, Newborn; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D008297:Male; D009305:Nasopharynx / Q000821:virology; D009975:Orthomyxoviridae; D019095:Point-of-Care Systems; D011237:Predictive Value of Tests; D018357:Respiratory Syncytial Virus Infections; D018113:Respiratory Syncytial Virus, Human; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology*; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology; D014780:Viruses / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral