title: Characterization of feline TRIM genes: molecular cloning, expression in tissues, and response to type I interferon.
abstract: Members of the tripartite motif (TRIM) protein family in mammals are responsible for various cellular processes. Previous studies have revealed that several TRIM proteins were induced by interferons (IFN) and that these proteins were involved in innate immune response against retroviral infection. Although retroviral infection is prevalent in domestic cats, the expression profiles and roles of feline TRIM genes against these viral infections are not well understood. In the present study, we examined tissue expression and IFN inducibility of nine feline TRIM genes. In addition, the complete coding sequences of six cloned TRIM genes were determined, and their structures were analyzed. Nine TRIM genes were expressed in feline tissues and five were up-regulated by type I IFN. The predicted amino acid sequence of six feline TRIM proteins showed high sequence similarities to other mammalian TRIM proteins, and suggest that feline TRIM genes are potentially involved in antiviral reactivity in IFN-mediated immune response.
authors.affiliation: Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Koba
authors.forename: Chika
authors.initials: C
authors.lastname: Kokaji
authors.forename: Gentoku
authors.initials: G
authors.lastname: Fujisaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Oguma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sentsui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D007370:Interferon Type I / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics*; D014777:Virus Diseases / Q000276:immunology / Q000662:veterinary*
chemical_list: D007370:Interferon Type I; D011506:Proteins