title: Luminescent material based on the [Eu(TTA)3(H2O)2] complex incorporated into modified silica particles for biological applications.
abstract: Amino-functionalized luminescent silica particles were investigated for use in immunoassays. The particles were prepared by the Stöber method where the [Eu(TTA)3(H2O)2] complex (TTA: 3-thenoyltrifluoroacetonate) was incorporated into silica particles during the hydrolysis and condensation of TEOS: tetraethylorthosilicate. Then, the amino groups were introduced in the particle surface using APTS: 3-aminopropyltriethoxisilane. The resulting particles were characterized by scanning electron microscopy (SEM), X ray diffraction (XRD) and photoluminescence spectroscopy. In order to demonstrate the viability of the use of luminescent particles as optical markers, an enzyme-substrate reaction was performed using HRP: horseradish peroxidase. It was possible to verify the binding of HRP-oxidized LDL (low density lipoprotein) and anti-oxLDL antibody-luminescent silica particles through the evaluation of the presence of HRP. The bioassay data open a broad field for the development of protein-tagged luminescent particles for use in biomedical sciences.
authors.affiliation: Instituto de Química, Universidade de São Paulo, CP 26077, 05508-000 São Paulo, Brazil.
authors.forename: Ana Valéria S
authors.initials: AV
authors.lastname: Lourenço
authors.forename: Cláudia A
authors.initials: CA
authors.lastname: Kodaira
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Ramos-Sanchez
authors.forename: Maria Cláudia F C
authors.initials: MC
authors.lastname: Felinto
authors.forename: Hiro
authors.initials: H
authors.lastname: Goto
authors.forename: Magnus
authors.initials: M
authors.lastname: Gidlund
authors.forename: Oscar L
authors.initials: OL
authors.lastname: Malta
authors.forename: Hermi F
authors.initials: HF
authors.lastname: Brito
mesh_terms: D001681:Biological Assay / Q000379:methods; D005063:Europium / Q000737:chemistry*; D049449:Luminescence; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D005063:Europium; D012822:Silicon Dioxide