title: Activity-dependent synaptic localization of processing bodies and their role in dendritic structural plasticity.
abstract: In neurons, transport of a subset of mRNAs to subcellular regions and their translation has a role in synaptic plasticity. Recent studies have suggested a control mechanism of this local translation through mRNA compartmentalization or degradation. Here we report that processing bodies (P-bodies), which are involved in mRNA degradation or storage, are transported to dendrites by conventional kinesin (KIF5A) as a motor protein. Neuronal activation induced by depolarization increased the colocalization of P-bodies with PSD-95 in dendrites. This neuronal activity increased the release of Nd1 and Arp2 mRNA from the P-bodies and, consequently, reversed the decrease of F-actin (induced by overexpression of Dcp1a) in the dendrites. Our data suggest that the activity-induced redistribution of P-bodies and mRNA release from P-bodies might have a role in synaptic structural plasticity by altering levels of mRNAs that are involved in the dynamics of the actin cytoskeleton in dendrites.
authors.affiliation: Department of Medicine and Microbiology, College of Medicine, Signaling Disorder Research Center, Chungbuk National University, Cheongju, 361-763, The Republic of Korea.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Oh
authors.forename: Ara
authors.initials: A
authors.lastname: Kwon
authors.forename: Anna
authors.initials: A
authors.lastname: Jo
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: Yong-Sook
authors.initials: YS
authors.lastname: Goo
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Lee
authors.forename: Hyong Kyu
authors.initials: HK
authors.lastname: Kim
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D051377:Actin-Related Protein 2 / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003712:Dendrites / Q000235:genetics / Q000378:metabolism*; D002479:Inclusion Bodies / Q000235:genetics / Q000378:metabolism*; D016547:Kinesins / Q000096:biosynthesis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
chemical_list: D051377:Actin-Related Protein 2; D000199:Actins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C547919:Kif5A protein, rat; D016547:Kinesins
keywords: Actin cytoskeleton; Kinesin motor protein; P-bodies; PSD-95; Synaptic plasticity