title: Plasma viremia and cellular HIV-1 DNA persist despite autologous hematopoietic stem cell transplantation for HIV-related lymphoma.
abstract: A cure of HIV-1 has been achieved in one individual through allogeneic stem cell transplantation with a CCR5[INCREMENT]32 homozygous donor. Whether myeloablation and autologous stem cell transplantation for lymphoma in patients on suppressive antiretroviral therapy can eliminate HIV-1 reservoirs is unknown. Low-level plasma viremia and total HIV-1 DNA and 2-LTR circles in blood mononuclear cells were quantified after autologous transplantation in 10 patients on suppressive antiretroviral therapy using quantitative polymerase chain reaction assays capable of single-copy nucleic acid detection. Plasma viremia was detectable in 9 patients, whereas HIV-1 DNA was detectable in all 10 patients, indicating that HIV-1 had not been eliminated.
authors.affiliation: Division of Infectious Diseases, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Cillo
authors.forename: Amrita
authors.initials: A
authors.lastname: Krishnan
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Mitsuyasu
authors.forename: Deborah K
authors.initials: DK
authors.lastname: McMahon
authors.forename: Shirley
authors.initials: S
authors.lastname: Li
authors.forename: John J
authors.initials: JJ
authors.lastname: Rossi
authors.forename: John A
authors.initials: JA
authors.lastname: Zaia
authors.forename: John W
authors.initials: JW
authors.lastname: Mellors
mesh_terms: D000328:Adult; D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000097:blood*; D016325:HIV Long Terminal Repeat; D015497:HIV-1; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007963:Leukocytes, Mononuclear; D016483:Lymphoma, AIDS-Related / Q000097:blood* / Q000628:therapy / Q000821:virology; D008297:Male; D008875:Middle Aged; D019653:Myeloablative Agonists / Q000627:therapeutic use; D012367:RNA, Viral / Q000097:blood*; D014182:Transplantation, Autologous; D019562:Viral Load; D055815:Young Adult
chemical_list: D044966:Anti-Retroviral Agents; D004279:DNA, Viral; D019653:Myeloablative Agonists; D012367:RNA, Viral