title: [Construction and identification of a mouse spermatocyte-derived cell line with a stable expression of PIAS-NY].
abstract: OBJECTIVE
  To construct a lentiviral expression vector of the PIAS-NY gene, and establish a mouse spermatocyte-derived cell line with a stable overexpression of PIAS-NY.
  
  
  METHODS
  PIAS-NY was synthesized, amplified by PCR and cloned into the lentiviral vector expression plasmid pGC-FU. After digestion and sequencing, pGC-FU-PIAS-NY, pHelper 1.0 and pHelper 2.0 were co-transfected into 293T cells. Then the lentiviral particles were used to transfect the mouse spermatocyte-derived cells. The expression of the PIAS-NY protein was detected by Western blot.
  
  
  RESULTS
  We successfully constructed the lentiviral expression vector pGC-FU-PIAS-NY and established a mouse spermatocyte-derived cell line with a stable overexpression of PIAS-NY.
  
  
  CONCLUSIONS
  The construction of the lentiviral expression vector pGC-FU-PIAS-NY and the obtainment of stably transfected mouse spermatocyte-derived cells have paved the way for further studies on the roles of the PIAS-NY gene in spermatogenesis.
authors.affiliation: Department of Anatomy, Histology and Embryology, The Affiliated Hospital of Yangzhou University School of Medicine, Yangzhou, Jiangsu 225001, China. yzzkl@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Hong-Ya
authors.initials: HY
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Jia
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Hu
authors.forename: Yong-Ze
authors.initials: YZ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics; D008297:Male; D051379:Mice; D010957:Plasmids; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics*; D013090:Spermatocytes / Q000166:cytology*; D014162:Transfection
chemical_list: D050828:Protein Inhibitors of Activated STAT