title: Cell-laden hydrogels in integrated microfluidic devices for long-term cell culture and tubulogenesis assays.
abstract: A hydrogel biochip combining microfluidic mixing and orthogonal supplementation strategies is developed and validated to allow facile generation of libraries of optically transparent 3D culture microenvironments. Live, on-chip tracing of embryonic stem cell differentiation and endothelial cell tubulogenesis confirms that the platform can be used to both create communities of discrete 3D microenvironments as well as to locally monitor subsequent divergent responses at both single cell and multi-cell scales.
authors.affiliation: Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Dr. Urbana, IL 61801, USA.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Gabrielson
authors.forename: Amit V
authors.initials: AV
authors.lastname: Desai
authors.forename: Bhushan
authors.initials: B
authors.lastname: Mahadik
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Hofmann
authors.forename: Paul J A
authors.initials: PJ
authors.lastname: Kenis
authors.forename: Brendan A C
authors.initials: BA
authors.lastname: Harley
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels
keywords: biomaterials; microfluidics; stem cells; tissue engineering; tubulogenesis