title: DNA manipulation and separation in sublithographic-scale nanowire array.
abstract: Electrokinetic manipulations of biomolecules using artificial nanostructures within microchannels have proven capability for controlling the dynamics of biomolecules. Because there is an inherent spatial size limitation to lithographic technology, especially for nanostructures with a small diameter and high aspect ratio, manipulating a single small biomolecule such as in DNA elongation before nanopore sequencing is still troublesome. Here we show the feasibility for self-assembly of a nanowire array embedded in a microchannel on a fused silica substrate as a means to manipulate the dynamics of a single long T4-DNA molecule and also separate DNA molecules. High-resolution optical microscopy measurements are used to clarify the presence of fully elongated T4-DNA molecules in the nanowire array. The spatial controllability of sublithographic-scale nanowires within microchannels offers a flexible platform not only for manipulating and separating long DNA molecules but also for integrating with other nanostructures to detect biomolecules in methods such as nanopore sequencing.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, and FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. yasui@apchem.nagoya-u.ac.jp
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.forename: Sakon
authors.initials: S
authors.lastname: Rahong
authors.forename: Koki
authors.initials: K
authors.lastname: Motoyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanagida
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.forename: Masaki
authors.initials: M
authors.lastname: Kanai
authors.forename: Kentaro
authors.initials: K
authors.lastname: Doi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nagashima
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Masateru
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Satoyuki
authors.initials: S
authors.lastname: Kawano
authors.forename: Tomoji
authors.initials: T
authors.lastname: Kawai
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D008422:Materials Testing; D008846:Micromanipulation / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D010781:Photography / Q000379:methods; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D012822:Silicon Dioxide; D004247:DNA