title: Extracellular β-fructofuranosidase from Fusarium graminearum: stability of the spray-dried enzyme in the presence of different carbohydrates.
abstract: Microbial enzymes have been used for various biotechnological applications; however, enzyme stabilization remains a challenge for industries and needs to be considered. This study describes the effects of spray-drying conditions on the activity and stability of β-fructofuranosidase from Fusarium graminearum. The extracellular enzyme β-fructofuranosidase was spray dried in the presence of stabilizers, including starch (Capsul) (SC), microcrystalline cellulose (MC), trehalose (TR), lactose (LC) and β-cyclodextrin (CD). In the presence of TR (2% w/v), the enzymatic activity was fully retained. After 1 year of storage, 74% of the enzymatic activity was maintained with the CD stabilizer (10% w/v). The residual activity was maintained as high as 80% for 1 h at 70°C when MC, SC and CD (5% w/v) stabilizers were used. Spray drying with carbohydrates was effective in stabilizing the F. graminearum β-fructofuranosidase, improved enzymatic properties compared to the soluble enzyme and demonstrated a potential use in future biotechnology applications.
authors.affiliation: Instituto de Química de Araraquara, UNESP , Araraquara, São Paulo , Brazil.
authors.forename: Heloísa Bressan
authors.initials: HB
authors.lastname: Gonçalves
authors.forename: João Atílio
authors.initials: JA
authors.lastname: Jorge
authors.forename: Wanderley Pereira
authors.initials: WP
authors.lastname: Oliveira
authors.forename: Claudia Regina Fernandes
authors.initials: CR
authors.lastname: Souza
authors.forename: Luis Henrique Souza
authors.initials: LH
authors.lastname: Guimarães
mesh_terms: D002241:Carbohydrates / Q000737:chemistry; D003890:Desiccation; D004795:Enzyme Stability; D005079:Excipients / Q000737:chemistry; D005670:Fusarium / Q000737:chemistry / Q000201:enzymology*; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D005079:Excipients; D043324:beta-Fructofuranosidase