title: Organo-selenium-containing dental sealant inhibits bacterial biofilm.
abstract: Oral bacteria, including Streptococcus mutans and Streptococcus salivarius, contribute to tooth decay and plaque formation; therefore, it is essential to develop strategies to prevent dental caries and plaque formation. We recently showed that organo-selenium compounds covalently attached to different biomaterials inhibited bacterial biofilms. Our current study investigates the efficacy of an organo-selenium dental sealant (SeLECT-Defense(TM) sealant) in inhibiting S. mutans and S. salivarius biofilm formation in vitro. The organo-selenium was synthesized and covalently attached to dental sealant material via standard polymer chemistry. By colony-forming unit (CFU) assay and confocal microscopy, SeLECT-Defense(TM) sealant was found to completely inhibit the development of S. mutans and S. salivarius biofilms. To assess the durability of the anti-biofilm effect, we soaked the SeLECT-Defense(TM) sealant in PBS for 2 mos at 37°C and found that the biofilm-inhibitory effect was not diminished after soaking. To determine if organo-selenium inhibits bacterial growth under the sealant, we placed SeLECT-Defense sealant over a lawn of S. mutans. In contrast to a control sealant, SeLECT-Defense(TM) sealant completely inhibited the growth of S. mutans. These results suggest that the inhibitory effect of SeLECT-Defense(TM) sealant against S. mutans and S. salivarius biofilms is very effective and durable.
authors.affiliation: Departments of Medical Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
authors.forename: P
authors.initials: P
authors.lastname: Tran
authors.forename: A
authors.initials: A
authors.lastname: Hamood
authors.forename: T
authors.initials: T
authors.lastname: Mosley
authors.forename: T
authors.initials: T
authors.lastname: Gray
authors.forename: C
authors.initials: C
authors.lastname: Jarvis
authors.forename: D
authors.initials: D
authors.lastname: Webster
authors.forename: B
authors.initials: B
authors.lastname: Amaechi
authors.forename: T
authors.initials: T
authors.lastname: Enos
authors.forename: T
authors.initials: T
authors.lastname: Reid
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control*; D016566:Organoselenium Compounds / Q000008:administration & dosage* / Q000737:chemistry; D010895:Pit and Fissure Sealants / Q000737:chemistry* / Q000494:pharmacology; D013291:Streptococcus / Q000187:drug effects*
chemical_list: D016566:Organoselenium Compounds; D010895:Pit and Fissure Sealants