title: Formulation of a pectin gel that efficiently traps mycotoxin deoxynivalenol and reduces its bioavailability.
abstract: We aimed to develop a new food-processing approach using pectin to reduce gastrointestinal absorption of mycotoxins. When Ca(2+) is added to low-methoxyl pectin, a gel resembling an egg box-like structure forms that is able to trap certain molecules. We examined whether or not low-methoxyl amidated pectin (LMA) and low-methoxyl non-amidated pectin (LMNA) trapped the mycotoxin deoxynivalenol (DON) after being ingested. We first determined the trapping effects of LMA and LMNA on DON in vitro under conditions similar to those in the human stomach, with results showing that LMA gel trapped DON to a greater extent than the LMNA gel. We then performed in vivo experiments and demonstrated that the LMA gel containing DON reduced DON's absorption from the gastrointestinal tract. This new food-processing technique holds great promise for reducing the bioavailability of DON in contaminated food and may be useful in mitigating the effects of other mycotoxins.
authors.affiliation: Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158-8501, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Tamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakauma
authors.forename: Hiroko
authors.initials: H
authors.lastname: Furusawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kadota
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamata
authors.forename: Motohiro
authors.initials: M
authors.lastname: Nishijima
authors.forename: Seigo
authors.initials: S
authors.lastname: Itoh
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
mesh_terms: D019412:Anatomy, Cross-Sectional; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D005506:Food Contamination / Q000517:prevention & control; D005750:Gastric Juice / Q000378:metabolism; D005782:Gels / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D010368:Pectins / Q000378:metabolism* / Q000648:ultrastructure; D014255:Trichothecenes / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D005782:Gels; D014255:Trichothecenes; D010368:Pectins; C007262:deoxynivalenol; D002118:Calcium