title: Association between adipose tissue expression and serum levels of leptin and adiponectin in women with polycystic ovary syndrome.
abstract: We reviewed emerging evidence linking serum levels and adipose tissue expression of leptin and adiponectin in women with polycystic ovary syndrome (PCOS). Previous data obtained by our group from a sample of overweight/obese PCOS women and a control sample of normal weight controls, both stratified by BMI, were reanalyzed. Circulating levels of leptin and adiponectin were determined by commercially available enzyme-linked immunosorbent assays. Adipose tissue total RNA was reserve-transcripted into complementary DNA samples, which were used as templates for quantitative real-time PCR amplification. Positive correlations were found between serum and mRNA levels for both leptin (r = 0.321; P = 0.005) and adiponectin (r = 0.266; P = 0.024). Determination of leptin and adiponectin serum levels could serve as an indirect method to assess adipocyte production, since leptin and adiponectin are predominantly produced by subcutaneous adipocytes in women.
authors.affiliation: Unidade de Endocrinologia Ginecológica, Serviço de Endocrinologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brasil.
authors.forename: S B
authors.initials: SB
authors.lastname: Lecke
authors.forename: D M
authors.initials: DM
authors.lastname: Morsch
authors.forename: P M
authors.initials: PM
authors.lastname: Spritzer
mesh_terms: D052242:Adiponectin / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood*; D009765:Obesity / Q000097:blood; D011085:Polycystic Ovary Syndrome / Q000097:blood*; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D020738:Leptin