title: Rate constant of tension-induced pore formation in lipid membranes.
abstract: We investigated the effects of tension induced by micropipet aspiration on giant unilamellar vesicles (GUVs) composed of dioleoylphosphatidylglycerol (DOPG) and dioleoylphosphatidylcholine (DOPC). We analyzed the time course of the fraction of intact GUVs among all of the GUVs under constant tension σ and obtained the rate constants of pore formation kp(σ). To determine kp, we developed an approach using the mean first passage time. The fitting of the theoretical curves of kp versus σ to the experimental data determined the line tension of a prepore, Γ. The value of Γ of a DOPG/DOPC bilayer was smaller than that of a DOPC bilayer.
authors.affiliation: Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
authors.forename: Victor
authors.initials: V
authors.lastname: Levadny
authors.forename: Taka-aki
authors.initials: TA
authors.lastname: Tsuboi
authors.forename: Marina
authors.initials: M
authors.lastname: Belaya
authors.forename: Masahito
authors.initials: M
authors.lastname: Yamazaki
mesh_terms: D007700:Kinetics; D008563:Membrane Lipids / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D013500:Surface Tension; D013816:Thermodynamics; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D053835:Unilamellar Liposomes; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; C017251:1,2-oleoylphosphatidylcholine