title: Novel 16S rRNA methyltransferase RmtH produced by Klebsiella pneumoniae associated with war-related trauma.
abstract: Klebsiella pneumoniae strain MRSN2404 was isolated from the chronic wound of a soldier who had been wounded in Iraq in 2006. The strain displayed very high MICs of all aminoglycosides, including arbekacin. A gene encoding a novel 16S rRNA methyltransferase, now designated RmtH, was identified. RmtH had 64% identity with RmtB1 and RmtB2. rmtH was bracketed by two copies of ISCR2, which may have played a role in its mobilization.
authors.affiliation: Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: O'Hara
authors.forename: Patrick
authors.initials: P
authors.lastname: McGann
authors.forename: Erik C
authors.initials: EC
authors.lastname: Snesrud
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Clifford
authors.forename: Paige E
authors.initials: PE
authors.lastname: Waterman
authors.forename: Emil P
authors.initials: EP
authors.lastname: Lesho
authors.forename: Yohei
authors.initials: Y
authors.lastname: Doi
mesh_terms: D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D006801:Humans; D007493:Iraq; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014857:Warfare
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007527:Isoenzymes; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases