title: Molecular epidemiology and antibiotic susceptibility of livestock Brucella melitensis isolates from Naryn Oblast, Kyrgyzstan.
abstract: The incidence of human brucellosis in Kyrgyzstan has been increasing in the last years and was identified as a priority disease needing most urgent control measures in the livestock population. The latest species identification of Brucella isolates in Kyrgyzstan was carried out in the 1960s and investigated the circulation of Brucella abortus, B. melitensis, B. ovis, and B. suis. However, supporting data and documentation of that experience are lacking. Therefore, typing of Brucella spp. and identification of the most important host species are necessary for the understanding of the main transmission routes and to adopt an effective brucellosis control policy in Kyrgyzstan. Overall, 17 B. melitensis strains from aborted fetuses of sheep and cattle isolated in the province of Naryn were studied. All strains were susceptible to trimethoprim-sulfamethoxazole, gentamicin, rifampin, ofloxacin, streptomycin, doxycycline, and ciprofloxacin. Multilocus variable number tandem repeat analysis showed low genetic diversity. Kyrgyz strains seem to be genetically associated with the Eastern Mediterranean group of the Brucella global phylogeny. We identified and confirmed transmission of B. melitensis to cattle and a close genetic relationship between B. melitensis strains isolated from sheep sharing the same pasture.
authors.affiliation: State Veterinary Department, Bishkek, Kyrgyzstan.
authors.forename: Joldoshbek
authors.initials: J
authors.lastname: Kasymbekov
authors.forename: Joldoshbek
authors.initials: J
authors.lastname: Imanseitov
authors.forename: Marie
authors.initials: M
authors.lastname: Ballif
authors.forename: Nadia
authors.initials: N
authors.lastname: Schürch
authors.forename: Sandra
authors.initials: S
authors.lastname: Paniga
authors.forename: Paola
authors.initials: P
authors.lastname: Pilo
authors.forename: Mauro
authors.initials: M
authors.lastname: Tonolla
authors.forename: Cinzia
authors.initials: C
authors.lastname: Benagli
authors.forename: Kulyash
authors.initials: K
authors.lastname: Akylbekova
authors.forename: Zarima
authors.initials: Z
authors.lastname: Jumakanova
authors.forename: Esther
authors.initials: E
authors.lastname: Schelling
authors.forename: Jakob
authors.initials: J
authors.lastname: Zinsstag
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017347:Brucella melitensis / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology; D014644:Genetic Variation; D005838:Genotype; D007707:Kyrgyzstan / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D058889:Molecular Typing; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents