title: 3,3',5,5'-Tetramethylbenzidine in hypochlorous acid and taurine chloramine scavenging assays: interference of dimethyl sulfoxide and other vehicles.
abstract: This article shows how six vehicles interfere in the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hypochlorous acid (HOCl) and taurine chloramine in vitro. All the tested vehicles inhibited TMB oxidation by HOCl; dimethyl sulfoxide had a remarkable effect at concentrations as low as 0.00005% (v/v). Cremophor EL and ethanol inhibited TMB oxidation by taurine chloramine at concentrations higher than 0.05 and 25% (v/v), respectively; the other vehicles did not affect this reaction. The results will help to guide the choice of solvent for the TMB oxidation assay performed under viable experimental conditions for evaluation of the HOCl and taurine chloramine scavenging ability of drugs.
authors.affiliation: Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14040-903, Brazil. luciana_kabeya@yahoo.com.br
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Kabeya
authors.forename: Micássio F
authors.initials: MF
authors.lastname: Andrade
authors.forename: Fabiana
authors.initials: F
authors.lastname: Piatesi
authors.forename: Ana Elisa C S
authors.initials: AE
authors.lastname: Azzolini
authors.forename: Ana Cristina M
authors.initials: AC
authors.lastname: Polizello
authors.forename: Yara Maria
authors.initials: YM
authors.lastname: Lucisano-Valim
mesh_terms: D001560:Benzidines / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005990:Glycerol / Q000031:analogs & derivatives / Q000737:chemistry; D006997:Hypochlorous Acid / Q000737:chemistry*; D010084:Oxidation-Reduction; D011794:Quercetin / Q000737:chemistry; D013654:Taurine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D001560:Benzidines; D013654:Taurine; C021758:3,3',5,5'-tetramethylbenzidine; D000431:Ethanol; C043410:N-chlorotaurine; C000515:cremophor EL; D006997:Hypochlorous Acid; D011794:Quercetin; D005990:Glycerol; D004121:Dimethyl Sulfoxide