title: NTFD--a stand-alone application for the non-targeted detection of stable isotope-labeled compounds in GC/MS data.
abstract: CONCLUSIONS
  Most current stable isotope-based methodologies are targeted and focus only on the well-described aspects of metabolic networks. Here, we present NTFD (non-targeted tracer fate detection), a software for the non-targeted analysis of all detectable compounds derived from a stable isotope-labeled tracer present in a GC/MS dataset. In contrast to traditional metabolic flux analysis approaches, NTFD does not depend on any a priori knowledge or library information. To obtain dynamic information on metabolic pathway activity, NTFD determines mass isotopomer distributions for all detected and labeled compounds. These data provide information on relative fluxes in a metabolic network. The graphical user interface allows users to import GC/MS data in netCDF format and export all information into a tab-separated format.
  
  
  BACKGROUND
  NTFD is C++- and Qt4-based, and it is freely available under an open-source license. Pre-compiled packages for the installation on Debian- and Redhat-based Linux distributions, as well as Windows operating systems, along with example data, are provided for download at http://ntfd.mit.edu/.
authors.affiliation: Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Esch-Belval, Luxembourg.
authors.forename: Karsten
authors.initials: K
authors.lastname: Hiller
authors.forename: André
authors.initials: A
authors.lastname: Wegner
authors.forename: Daniel
authors.initials: D
authors.lastname: Weindl
authors.forename: Thekla
authors.initials: T
authors.lastname: Cordes
authors.forename: Christian M
authors.initials: CM
authors.lastname: Metallo
authors.forename: Joanne K
authors.initials: JK
authors.lastname: Kelleher
authors.forename: Gregory
authors.initials: G
authors.lastname: Stephanopoulos
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007553:Isotope Labeling; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods*; D012984:Software