title: Treatment of FGF-2 on stem cells from inflamed dental pulp tissue from human deciduous teeth.
abstract: OBJECTIVE
  The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue of human functional deciduous teeth (iSHFD) and to evaluate the influence of fibroblastic growth factor-2 (FGF-2) on the regenerative potential.
  
  
  METHODS
  We successfully isolated mesenchymal stem cells (MSCs) from the inflamed dental pulp tissue of human deciduous teeth and demonstrated that their regenerative potential could be enhanced by the application of FGF-2 (20 ng ml(-1)) during ex vivo expansion. Isolated stem cells expanded in FGF-2 were characterized using a colony-forming assay, proliferation, migration, in vitro differentiation, in vivo ectopic transplantation assay, and gene expression profiling.
  
  
  RESULTS
  MSCs isolated from the inflamed pulp tissue of functional deciduous teeth potentially possess the qualities of those from human exfoliated deciduous teeth. FGF-2 applied to iSHFD during expansion enhanced the colony-forming efficiency of these cells, increased their proliferation and migration potential, and reduced their differentiation potential in vitro. However, the ectopic transplantation of iSHFD/FGF-2 in vivo increased the formation of dentin-like material.
  
  
  CONCLUSIONS
  FGF-2 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: Jc
authors.initials: J
authors.lastname: Kim
authors.forename: J C
authors.initials: JC
authors.lastname: Park
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: G I
authors.initials: GI
authors.lastname: Im
authors.forename: B S
authors.initials: BS
authors.lastname: Kim
authors.forename: J B
authors.initials: JB
authors.lastname: Lee
authors.forename: E Y
authors.initials: EY
authors.lastname: Choi
authors.forename: J S
authors.initials: JS
authors.lastname: Song
authors.forename: K S
authors.initials: KS
authors.lastname: Cho
authors.forename: C S
authors.initials: CS
authors.lastname: Kim
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D011671:Pulpitis / Q000473:pathology*; D014094:Tooth, Deciduous
chemical_list: D016222:Fibroblast Growth Factor 2
keywords: biomedical engineering; inflammatory/immune response and modulation; mesenchymal stem cells; tissue development and growth