title: Porcine CD4 promoters and enhancers can direct foreign gene expression in human cells.
abstract: Porcine CD4 proximal promoter and enhancer sequences were cloned and aligned with the corresponding human and murine sequences. The alignment showed nucleotide homology between porcine and human sequences was 62.4 % for the CD4 promoter and 56.6 % for the CD4 enhancer. The nucleotide homology between porcine and murine sequences was 42.5 % for the CD4 promoter and 25.4 % for the CD4 enhancer. The proximal enhancer and promoter regions of the CD4 gene from porcine, murine and human cells were compared for their ability to direct foreign gene expression in transiently transfected human cell lines. The results indicated the porcine CD4 promoters and enhancers could effectively direct expression of a foreign gene in human cells. The porcine promoter was equally efficient as CMV and EF-1α in directing gene expression.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Lin
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Fuwang
authors.initials: F
authors.lastname: Chen
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Cao
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Meng
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Daxing
authors.initials: D
authors.lastname: Pang
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.forename: Linzhu
authors.initials: L
authors.lastname: Ren
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D005434:Flow Cytometry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics
chemical_list: D015704:CD4 Antigens; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins