title: Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing.
abstract: OBJECTIVE
  To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37°C on TCBS medium, in September 2009 from seawater and surface sediments.
  
  
  RESULTS
  q-PCR assays previously selected for the identification of bacterial strains isolated at 37°C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22°C. The great majority of the 37°C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85·9%) and V. harveyi (10·7%). The human pathogens V. parahaemolyticus and V. cholerae were rarely detected (two strains each). The 22°C strains were successfully identified by the phylogeny analysis of pyrH and toxR genes, revealing 20 Vibrio species, with the predominance of the clam pathogen V. celticus (36·8%). The Splendidus and the Harveyi groups represented the main Vibrio group at 22°C (80%) and 37°C (99·5%), respectively.
  
  
  CONCLUSIONS
  The combination of q-PCR assays and the sequencing of pyrH and toxR genes highlighted two different Vibrio communities at 22 and 37°C both dominated by pathogenic species for marine organisms.
  
  
  CONCLUSIONS
  The sequencing of the pyrH gene revealed to be a valuable tool to identify environmental Vibrio spp. strains isolated at 22°C, as 92·3% of them were identified in this study.
authors.affiliation: Laboratoire Microbiologie-LNR, Unité Environnement, Microbiologie et Phycotoxines, Département Ressources Biologiques et Environnement, Centre de Brest, Ifremer, Plouzané, France.
authors.forename: A
authors.initials: A
authors.lastname: Tall
authors.forename: D
authors.initials: D
authors.lastname: Hervio-Heath
authors.forename: A
authors.initials: A
authors.lastname: Teillon
authors.forename: C
authors.initials: C
authors.lastname: Boisset-Helbert
authors.forename: R
authors.initials: R
authors.lastname: Delesmont
authors.forename: J
authors.initials: J
authors.lastname: Bodilis
authors.forename: A
authors.initials: A
authors.lastname: Touron-Bodilis
mesh_terms: D044822:Biodiversity; D005798:Genes, Bacterial; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044149:Vibrio alginolyticus / Q000302:isolation & purification