title: Anti-tuberculosis neolignans from Piper regnellii.
abstract: The present study determined the anti-Mycobacterium tuberculosis activities of supercritical CO2 extracts, neolignans eupomatenoid-5 (1), conocarpan (4) and eupomatenoid-3 (7) and their derivatives (2, 3, 5, 6, and 8) from Piper regnellii, as well as their cytotoxicities. The supercritical CO2 extract from leaves was purified by chromatographic methods, yielding compounds (1), (4) and (7), which were identified by (1)H NMR and comparison with literature data. Anti-M. tuberculosis activity (H37Rv and clinical isolates) was evaluated using a resazurin microtiter assay plate (REMA) to determine the MIC. The cytotoxicity assay was carried out in macrophages J774G.8 by sulforhodamine B colorimetric assay. The supercritical CO2 extracts from leaves and stems, and compound (4) showed activity against M. tuberculosis (MIC 15.6 μg/ml). Compound (1) showed the best activity (MIC 1.9 μg/ml), with good SI. Compounds (7) and (8) showed low activity against M. tuberculosis H37Rv. The derivative compounds did not show increased anti-M. tuberculosis activity. This is the first report, to our knowledge, to describe neolignans from P. regnellii with activity against M. tuberculosis, and compound (1) is a potential candidate for future antituberculosis drugs.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: R B L
authors.initials: RB
authors.lastname: Scodro
authors.forename: C T A
authors.initials: CT
authors.lastname: Pires
authors.forename: V S
authors.initials: VS
authors.lastname: Carrara
authors.forename: C O T
authors.initials: CO
authors.lastname: Lemos
authors.forename: L
authors.initials: L
authors.lastname: Cardozo-Filho
authors.forename: V A
authors.initials: VA
authors.lastname: Souza
authors.forename: A G
authors.initials: AG
authors.lastname: Corrêa
authors.forename: V L D
authors.initials: VL
authors.lastname: Siqueira
authors.forename: M V C
authors.initials: MV
authors.lastname: Lonardoni
authors.forename: R F
authors.initials: RF
authors.lastname: Cardoso
authors.forename: D A G
authors.initials: DA
authors.lastname: Cortez
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000737:chemistry / Q000494:pharmacology*; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line / Q000187:drug effects; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*; D018675:Toxicity Tests / Q000379:methods
chemical_list: D000995:Antitubercular Agents; D001572:Benzofurans; D017705:Lignans; D010636:Phenols; D010936:Plant Extracts; C584401:eupomatenoid-3; C447284:conocarpan; C452811:eupomatenoid 5