title: Effect of fibrin glue on antioxidant defense mechanism, oxidative DNA damage and chromosomal aberrations.
abstract: Oxidative stress is involved in diverse biological phenomenon, and is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is the most critical biomarker in the estimation of ROS-induced DNA damage. This investigation focuses on the effect of fibrin glue on lipid peroxidation (LPO), antioxidant enzymes and oxidative DNA damage (both in vitro and in vivo). The blood biochemical parameters of the implanted animals and in vitro chromosomal aberrations were also studied. Fibrin glue was applied on the calvarial defect made on the anesthetized rats for an observation period of 4, 12, 26 and 52 weeks. At the end of the observation period, animals were anesthetized; blood was collected for serum analysis and was sacrificed. Brain was collected for the detection of 8-OHdG using competitive ELISA and liver was collected for analyzing the antioxidant enzymes and LPO. The results of this study suggest that the effect of fibrin glue on rat brain (in vivo and in vitro) and mice liver (in vitro) did not make any significant influence on LPO and antioxidant defense system. Similarly, there was no change observed in the expression of 8-OHdG. Serum constituents of implanted rats were observed to be within the normal range. The normal karyotype obtained indicates that the physiological saline extract of fibrin glue does not induce any chromosomal anomalies. Hence, it was concluded that the fibrin glue material does not have any potential to produce oxidative stress, alterations in the C-8 position of guanine and chromosomal anomalies.
authors.affiliation: Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
authors.forename: V S
authors.initials: VS
authors.lastname: Anjana Vaman
authors.forename: S K
authors.initials: SK
authors.lastname: Tinu
authors.forename: C S
authors.initials: CS
authors.lastname: Geetha
authors.forename: K K
authors.initials: KK
authors.lastname: Lissy
authors.forename: P V
authors.initials: PV
authors.lastname: Mohanan
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005337:Fibrin / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D007621:Karyotyping; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014014:Tissue Adhesives / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014014:Tissue Adhesives; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005337:Fibrin; D003849:Deoxyguanosine; D005978:Glutathione