title: Photonic wire biosensor microarray chip and instrumentation with application to serotyping of Escherichia coli isolates.
abstract: A complete photonic wire molecular biosensor microarray chip architecture and supporting instrumentation is described. Chip layouts with 16 and 128 independent sensors have been fabricated and tested, where each sensor can provide an independent molecular binding curve. Each sensor is 50 μm in diameter, and consists of a millimeter long silicon photonic wire waveguide folded into a spiral ring resonator. An array of 128 sensors occupies a 2 × 2 mm2 area on a 6 × 9 mm2 chip. Microfluidic sample delivery channels are fabricated monolithically on the chip. The size and layout of the sensor array is fully compatible with commercial spotting tools designed to independently functionalize fluorescence based biochips. The sensor chips are interrogated using an instrument that delivers sample fluid to the chip and is capable of acquiring up to 128 optical sensor outputs simultaneously and in real time. Coupling light from the sensor chip is accomplished through arrays of sub-wavelength surface grating couplers, and the signals are collected by a fixed two-dimensional detector array. The chip and instrument are designed so that connection of the fluid delivery system and optical alignment are automated, and can be completed in a few seconds with no active user input. This microarray system is used to demonstrate a multiplexed assay for serotyping E. coli bacteria using serospecific polyclonal antibody probe molecules.
authors.affiliation: National Research Council Canada (NRC), Ottawa, Ontario, K1A 0R6, Canada. siegfried.janz@nrc-cnrc.gc.ca
authors.forename: S
authors.initials: S
authors.lastname: Janz
authors.forename: D-X
authors.initials: DX
authors.lastname: Xu
authors.forename: M
authors.initials: M
authors.lastname: Vachon
authors.forename: N
authors.initials: N
authors.lastname: Sabourin
authors.forename: P
authors.initials: P
authors.lastname: Cheben
authors.forename: H
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authors.lastname: McIntosh
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authors.initials: H
authors.lastname: Ding
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: J H
authors.initials: JH
authors.lastname: Schmid
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authors.lastname: Delâge
authors.forename: J
authors.initials: J
authors.lastname: Lapointe
authors.forename: A
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authors.lastname: Densmore
authors.forename: R
authors.initials: R
authors.lastname: Ma
authors.forename: W
authors.initials: W
authors.lastname: Sinclair
authors.forename: S M
authors.initials: SM
authors.lastname: Logan
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authors.initials: R
authors.lastname: Mackenzie
authors.forename: Q Y
authors.initials: QY
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Lopinski
authors.forename: O
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authors.lastname: Mozenson
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authors.lastname: Gilmour
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authors.lastname: Tabor
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000302:isolation & purification*; D010783:Photometry / Q000295:instrumentation*; D012703:Serotyping / Q000295:instrumentation*; D046888:Tissue Array Analysis / Q000295:instrumentation*