title: Treatment with 670 nm light up regulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model.
abstract: Inflammation is an umbrella feature of ageing. It is present in the aged retina and many retinal diseases including age-related macular degeneration (AMD). In ageing and in AMD mitochondrial function declines. In normal ageing this can be manipulated by brief exposure to 670 nm light on the retina, which increases mitochondrial membrane potential and reduces inflammation. Here we ask if 670 nm exposure has the same ability in an aged mouse model of AMD, the complement factor H knockout (CFH(-/-)) where inflammation is a key feature. Further, we ask whether this occurs when 670 nm is delivered briefly in environmental lighting rather than directly focussed on the retina. Mice were exposed to 670 nm for 6 minutes twice a day for 14 days in the form of supplemented environmental light. Exposed animals had significant increase in cytochrome c oxidase (COX), which is a mitochondrial enzyme regulating oxidative phosphorylation.There was a significant reduction in complement component C3, an inflammatory marker in the outer retina. Vimetin and glial fibrillary acidic protein (GFAP) expression, which reflect retinal stress in Muller glia, were also significantly down regulated. There were also significant changes in outer retinal macrophage morphology. However, amyloid beta (Aβ) load, which also increases with age in the outer retina and is pro-inflammatory, did not change. Hence, 670 nm is effective in reducing inflammation probably via COX activation in mice with a genotype similar to that in 50% of AMD patients even when brief exposures are delivered via environmental lighting. Further, inflammation can be reduced independent of Aβ. The efficacy revealed here supports current early stage clinical trials of 670 nm in AMD patients.
authors.affiliation: Institute of Ophthalmology, University College London, London, United Kingdom.
authors.forename: Rana
authors.initials: R
authors.lastname: Begum
authors.forename: Michael B
authors.initials: MB
authors.lastname: Powner
authors.forename: Natalie
authors.initials: N
authors.lastname: Hudson
authors.forename: Chris
authors.initials: C
authors.lastname: Hogg
authors.forename: Glen
authors.initials: G
authors.lastname: Jeffery
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects*; D007249:Inflammation / Q000201:enzymology / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008027:Light; D008264:Macrophages / Q000473:pathology / Q000528:radiation effects; D008268:Macular Degeneration / Q000201:enzymology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051379:Mice; D018384:Oxidative Stress / Q000528:radiation effects; D010789:Phototherapy / Q000379:methods*; D012160:Retina / Q000378:metabolism / Q000528:radiation effects; D015854:Up-Regulation / Q000528:radiation effects*
chemical_list: D003576:Electron Transport Complex IV