title: MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia.
abstract: Matrix metallinoprotease-9 (MMP9) plays a key role in the pathogenesis of post-ischemic blood brain barrier (BBB) disruption and the formation of lesions after cerebral ischemia. In this study we investigate the effect of brain-specific miRNAs on MMP-9 protein level in the rat hippocampus following cerebral ischemia and its underlying mechanism. Cerebral ischemia significantly upregulated miR-21 and -224 in the hippocampus; however, expression of miR-122 and -338-3p was not significantly affected by ischemia. Silencing of miR-21, but not -224, reduced MMP9 protein level after cerebral ischemia. Downregulation of extracellular signal-regulated kinase (ERK) signaling using the ERK inhibitor U0126 and the calcium-channel blocker ketamine inhibited the upregulation of miR-21 expression and MMP9 protein level after cerebral ischemia. The study suggests that cerebral ischemia up-regulates expression level of miR-21, which is involved in ERK-stimulated upregulation of MMP9 following cerebral ischemia via a calcium-dependent mechanism.
authors.affiliation: Department of Neurology, Puai Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430033, PR China.
authors.forename: XiaHeng
authors.initials: X
authors.lastname: Deng
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhong
authors.forename: LiZe
authors.initials: L
authors.lastname: Gu
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D035683:MicroRNAs / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C546082:mirn21 microRNA, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat