title: γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.
abstract: Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.
authors.affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.
authors.forename: Jing-Shu
authors.initials: JS
authors.lastname: Zhang
authors.forename: Da-Ming
authors.initials: DM
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.forename: Ning
authors.initials: N
authors.lastname: He
authors.forename: Qing
authors.initials: Q
authors.lastname: Gu
authors.forename: Feng-Shan
authors.initials: FS
authors.lastname: Wang
authors.forename: Shu-Qing
authors.initials: SQ
authors.lastname: Jiang
authors.forename: Bing-Qing
authors.initials: BQ
authors.lastname: Chen
authors.forename: Jia-Ren
authors.initials: JR
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002839:Chromans / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000473:pathology*; D004789:Enzyme Activation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D002839:Chromans; D051153:Wnt Proteins; D014810:Vitamin E; C013649:plastochromanol 8; D053148:Caspase 3