title: Detection of border disease virus (BDV) in goat herds suffering diarrhea in eastern China.
abstract: BACKGROUND
  Border disease virus (BDV) is an important pathogen in sheep and goat production. Neither epidemiological investigation nor any reports of BDV infection was available in China. During Jan to Apr, 2012, several herd goats in Anhui and Jiangsu provinces in eastern China suffered unremitting diarrhea, with morbidity and mortality of about 28-37% and 10-15%, respectively. In the present study, sera and tissue samples from diseased goats of four farms were taken for BDV detection, isolation and identification.
  
  
  RESULTS
  Panpesti generic primers and border disease virus (BDV)-specific primers targeting the 5'-UTR region produced RT-PCR positive bands for sera (24/28) and tissue samples (7/30). Twenty positive sera and tissue samples were inoculated onto Madin-Darby bovine kidney (MDBK) cells for virus isolation. Finally, three different strains of BDV, named AH12-01, AH12-02 and JS12/04, were successfully isolated as identified by RT-PCR using 5'-UTR and N(pro) gene primers, sequencing and electron microscopy. Sequences of 5'-UTR and N(pro) genes of them were used for phylogenetic analysis and comparison to other reference sequences available in GenBank. The results indicated AH12-01, AH12-02 and JS12/04 possess high relationship with the BDV 3 group viruses and differed with each other.
  
  
  CONCLUSIONS
  This is the first detection of BDV from goats with diarrhea and confirmation of BDV infection in China.
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Sun
authors.forename: Kongwang
authors.initials: K
authors.lastname: He
authors.forename: Jieyuan
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001882:Border Disease / Q000473:pathology / Q000821:virology*; D018083:Border disease virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D003967:Diarrhea / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D015511:Goat Diseases / Q000473:pathology / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins