title: Electrophysiological study for comparing the effect of biological activity between type A botulinum toxins in rat gastrocnemius muscle.
abstract: BACKGROUND
  New cosmetic applications and products based on the effects of botulinum toxin (BTX) treatment have stimulated demand for this class of natural compounds. This demand generates the need for appropriate standardized protocols to test and compare the effectiveness of new BTX preparations.
  
  
  OBJECTIVE
  Based on the previously described electrophysiological methods, we measured and compared the inhibitory effects of two BTX type A (BTX-A) preparations on neuromuscular transmission through split-body test.
  
  
  METHODS
  The effectiveness was evaluated in terms of the compound muscle action potential (CMAP) and conduction velocity after BTX-A injection. We used a split-body method to compare two different BTX-As in the rat.
  
  
  RESULTS
  Based on the changes in the CMAP, the two different BTX-As induced paralytic effect on the rat tibialis anterior muscle. However, the two different BTX-A preparations did not differ significantly in effectiveness and did not induce a delay in conduction velocity.
  
  
  CONCLUSIONS
  The new BTX-A preparation used in this electrophysiological study had similar effect compared with the previously marketed BTX-A.[AQ: Please approve the edits made to the sentence "The new BTX-A preparation…") We propose that a split-body electrophysiological protocol will be useful in establishing the comparative effectiveness of new BTX products.
authors.affiliation: Daewoong Pharmaceutical Co., Ltd, Seoul, Republic of Korea.
authors.forename: C-S
authors.initials: CS
authors.lastname: Kim
authors.forename: W S
authors.initials: WS
authors.lastname: Jang
authors.forename: I P
authors.initials: IP
authors.lastname: Son
authors.forename: S H
authors.initials: SH
authors.lastname: Nam
authors.forename: Y I
authors.initials: YI
authors.lastname: Kim
authors.forename: K Y
authors.initials: KY
authors.lastname: Park
authors.forename: B J
authors.initials: BJ
authors.lastname: Kim
authors.forename: M N
authors.initials: MN
authors.lastname: Kim
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D019274:Botulinum Toxins, Type A / Q000008:administration & dosage / Q000494:pharmacology*; D004553:Electric Conductivity; D004558:Electric Stimulation; D004594:Electrophysiology / Q000379:methods; D007273:Injections, Intramuscular; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000294:innervation / Q000502:physiology; D009465:Neuromuscular Agents / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: C586826:DWP450; D009465:Neuromuscular Agents; D019274:Botulinum Toxins, Type A
keywords: Botulinum toxin; compound muscle action potential; toxicity assay