title: Bioactive pregnane steroids from a South China Sea gorgonian Carijoa sp.
abstract: A new pregnane steroid, 1, and three known analogues 2-4, have been isolated from a gorgonian Carijoa sp. collected from the South China Sea. The planar structure and relative configuration of 1 were elucidated from comprehensive spectroscopic data. Its absolute configuration was determined by application of the modified Mosher method. Compounds 1, 3 and 4 exhibited cytotoxicity against the human hepatoma cell line Bel-7402, with IC50 values of 9.33, 11.02 and 18.68 µM, respectively. Additionally, compound 1 exhibited promising antibacterial activity against Pseudomona puido, with a MIC value of 31 nM, which is approximately 5-fold more potent than ciprofloxacin (MIC = 156 nM).
authors.affiliation: Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. zhaohongying786@126.com
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Zhao
authors.forename: Chang-Lun
authors.initials: CL
authors.lastname: Shao
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Fei
authors.initials: F
authors.lastname: Cao
authors.forename: Chang-Yun
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002681:China; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008968:Molecular Conformation; D009792:Oceans and Seas; D011281:Pregnatrienes / Q000302:isolation & purification* / Q000494:pharmacology; D016958:Pseudomonas putida / Q000187:drug effects
chemical_list: C583170:15beta-hydroxypregna-1,4,20-trien-3-one; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D011281:Pregnatrienes