title: Novel insight into Y-box binding protein 1 in the regulation of vascular smooth muscle cell proliferation through targeting GC box-dependent genes.
abstract: Abnormal proliferation of vascular smooth muscle cells (VSMCs) is a key event in atherosclerosis and restenosis. In this paper, we report that Y-box binding protein 1 (YB1) functions as a phenotypic regulator in VSMC proliferation-differentiation switching through targeting GC box-dependent genes. Oligo pull-down assays demonstrated that YB1 binds directly to GC boxes via amino acids 125-220. YB1 C-terminal tail domain (CTD, amino acids 125-324) regulates GC box-dependent target gene transcription and suppresses VSMC proliferation. These findings provide a novel insight into the regulation of GC box-related genes by YB1, and provide a new understanding of VSMC proliferation regulation.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Key Laboratory of Neurobiology and Vascular Biology, China Administration of Education, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Jian-hong
authors.initials: JH
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.forename: Yong-hui
authors.initials: YH
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Ai-li
authors.initials: AL
authors.lastname: Han
authors.forename: Xin-hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Xin-rui
authors.initials: XR
authors.lastname: Lv
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.forename: Jin-kun
authors.initials: JK
authors.lastname: Wen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D059372:Nucleotide Motifs / Q000187:drug effects / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology; D051840:Y-Box-Binding Protein 1 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009841:Oligonucleotides; D051840:Y-Box-Binding Protein 1; D014212:Tretinoin