title: Screening for cytochrome P450 reactivity with a reporter enzyme.
abstract: The identification of novel substrates of cytochrome P450 enzymes by high-throughput screening assays is of utmost importance to further increase the scope of these enzymes for future applications. Most screens are either confined to individual substrate analogues or hampered by low throughput due to elaborate analysis techniques. Here we describe a general high-throughput screening assay that interrogates the activity of P450 enzymes with the aid of catalase as a reporter enzyme.
authors.affiliation: Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Dortmund, Germany.
authors.forename: Kersten S
authors.initials: KS
authors.lastname: Rabe
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D002138:Calibration; D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D008958:Models, Molecular; D010078:Oxazines / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D010078:Oxazines; C014180:resorufin; D003577:Cytochrome P-450 Enzyme System; D002374:Catalase