title: Amurensin G induces autophagy and attenuates cellular toxicities in a rotenone model of Parkinson's disease.
abstract: Although Parkinson's disease is a common neurodegenerative disorder its cause is still unknown. Recently, several reports showed that inducers of autophagy attenuate cellular toxicities in Parkinson's disease models. In this report we screened HEK293 cells that stably express GFP-LC3, a marker of autophagy, for autophagy inducers and identified amurensin G, a compound isolated from the wild grape (Vitis amurensis). Amurensin G treatment induced punctate cytoplasmic expression of GFP-LC3 and increased the expression level of endogenous LC3-II. Incubation of human dopaminergic SH-SY5Y cells with amurensin G attenuated the cellular toxicities of rotenone in a model of Parkinson's disease. Amurensin G inhibited rotenone-induced apoptosis and interfered with rotenone-induced G2/M cell cycle arrest. In addition, knockdown of beclin1, a regulator of autophagy, abolished the effect of amurensin G. These data collectively indicate that amurensin G attenuates cellular toxicities through the induction of autophagy.
authors.affiliation: Division of Biological Science and Technology, Yonsei University, Wonju, Republic of Korea.
authors.forename: Hyun-Wook
authors.initials: HW
authors.lastname: Ryu
authors.forename: Won Keun
authors.initials: WK
authors.lastname: Oh
authors.forename: Ik-Soon
authors.initials: IS
authors.lastname: Jang
authors.forename: Junsoo
authors.initials: J
authors.lastname: Park
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D001343:Autophagy / Q000187:drug effects*; D001483:Base Sequence; D000071186:Beclin-1; D003986:Dibenzocycloheptenes / Q000302:isolation & purification / Q000494:pharmacology*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D055670:Medicine, Korean Traditional; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D008954:Models, Biological; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity; D010300:Parkinson Disease / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics; D012118:Resorcinols / Q000302:isolation & purification / Q000494:pharmacology*; D012402:Rotenone / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D027843:Vitis / Q000737:chemistry
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D003986:Dibenzocycloheptenes; D008565:Membrane Proteins; D009498:Neurotoxins; D034741:RNA, Small Interfering; D012118:Resorcinols; C553921:amurensin G; D012402:Rotenone