title: [Analysis of influence factors on G-CSF-mobilized hematopoietic stem cells of 191 healthy donors in a mono center].
abstract: This study was aimed to investigate the factors influencing mobilization efficiency of peripheral hematopoietic stem cells with granulocyte colony stimulating factor (G-CSF) and their impact on healthy donors. 181 donors were mobilized subcutaneously with G-CSF at 5 - 10 µg/(kg·d), and 10 donors were mobilized with G-CSF at 3.3 - 4.9 µg/(kg·d), once 12 h, for 4 - 5 d. Peripheral blood mononuclear cell (MNC) and CD34(+) cell counts were analyzed by flow cytometry. Mobilization-related side effects were also monitored. The results showed that white blood cell counts increased by 6 times averaged after mobilization (P < 0.01). The platelet count obviously decreased (P < 0.01), while the hemoglobin level did not show significant difference. No significant differences were observed in MNC and CD34(+) cell counts between those subjects harvested on the 4th and 5th day after mobilization. Male donors were superior to female ones in cell harvest (P < 0.01). Donor body weight played positive role in cell yield, while impact of age on harvest was not remarkable. Neither MNC nor CD34(+) cell count showed a linear relationship with G-CSF dose. Only slight side effects were observed on the donors in this study. It is concluded that mobilization with G-CSF is sufficient in healthy donors without remarkable side effects.
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Yuan
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Bo
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Wang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Dou
authors.forename: Quan-Shun
authors.initials: QS
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Yu
authors.initials: Y
authors.lastname: Jing
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D005163:Factor Analysis, Statistical; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D016179:Granulocyte Colony-Stimulating Factor