title: Two novel potent α-amylase inhibitors from the family of acarviostatins isolated from the culture of Streptomyces coelicoflavus ZG0656.
abstract: Two novel aminooligosaccharides were separated from the culture filtrate of Streptomyces coelicoflavus ZG0656. Their chemical structures were determined by acidic hydrolysis, electrospray-ionization tandem mass spectrometry (ESI-MS/MS), and NMR spectroscopy. The compounds were named acarviostatins III0(-1) and III23 according to the nomenclature of this group of metabolites. The two novel acarviostatins were both mixed noncompetitive inhibitors of porcine pancreatic α-amylase (PPA). The inhibition constants (K(i)) for acarviostatins III0(-1) and III23 were 0.009 and 0.026 μM, respectively, 151 and 52 times more potent than acarbose.
authors.affiliation: Tianjin State Laboratory of Cellular and Molecular Immunology, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, PR China. gengpeng_2001@163.com
authors.forename: Peng
authors.initials: P
authors.lastname: Geng
authors.forename: Ting
authors.initials: T
authors.lastname: Sun
authors.forename: Qiping
authors.initials: Q
authors.lastname: Zhong
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Li
authors.forename: Liying
authors.initials: L
authors.lastname: Shi
authors.forename: Fang
authors.initials: F
authors.lastname: Bai
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
mesh_terms: D002240:Carbohydrate Sequence; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D011134:Polysaccharides; C583432:acarviostatin III0(-1); C583433:acarviostatin III23; D055292:Pancreatic alpha-Amylases