title: Ascorbigen induces dermal papilla cell proliferation in vitro, but fails to modulate chemotherapy-induced alopecia in vivo.
abstract: Ascorbigen (ABG) is the predominant indole-derived compound from Brassica vegetables. In this study, we attempted to evaluate the effects of ABG on hair growth. To this end, we examined the proliferation of isolated human dermal papilla (DP) cells and keratinocytes after incubation in various concentrations (0-1.25 mM) of ABG. Furthermore, hair shaft regrowth was monitored in a mouse model of chemotherapy-induced alopecia (CIA), and hematoxylin and eosin staining was performed for histological analyses. We found that 1.25 mM ABG induced a 1.2-fold increase in the growth of DP cells, but not keratinocytes. However, ABG did not exert significant protective effects against CIA in the mouse model. These findings suggest that ABG may not be able to counteract CIA and that further investigation of the therapeutic potential of ABG in disease models is required.
authors.affiliation: Rheumatology/Immunology/Allergy, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Chih-Hsiu
authors.initials: CH
authors.lastname: Wang
authors.forename: Hsu-Shan
authors.initials: HS
authors.lastname: Huang
authors.forename: Niann-Tzyy
authors.initials: NT
authors.lastname: Dai
authors.forename: Ming-Jen
authors.initials: MJ
authors.lastname: Sheu
authors.forename: Deh-Ming
authors.initials: DM
authors.lastname: Chang
mesh_terms: D000505:Alopecia / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D001937:Brassica / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000009:adverse effects; D020405:Dermis / Q000166:cytology*; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D007211:Indoles; C053152:ascorbigen; D003520:Cyclophosphamide; D001205:Ascorbic Acid
keywords: ascorbigen; chemotherapy-induced alopecia; dermal papilla