title: Melanoblasts as multipotent cells in murine skin.
abstract: Melanoblasts are melanocyte precursors that are derived from neural crest cells (NCCs). Recently we showed that melanoblasts differentiate into not only pigmented melanocytes but also into other NCCs derivatives. Here, we describe methods for the isolation of melanoblasts from mouse skin by flow-cytometry. Methods for culturing the isolated melanoblasts, allowing them to express their multipotentiality, are also described.
authors.affiliation: Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Motohashi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kunisada
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D012867:Skin / Q000166:cytology / Q000378:metabolism
chemical_list: D017493:Leukocyte Common Antigens