title: ADAM9 silencing inhibits breast tumor cell invasion in vitro.
abstract: ADAM9 (A Disintegrin And Metalloproteinase 9) is a member of the ADAM protein family which contains a disintegrin domain. This protein family plays key roles in many physiological processes, including fertilization, migration, and cell survival. The ADAM proteins have also been implicated in various diseases, including cancer. Specifically, ADAM9 has been suggested to be involved in metastasis. To address this question, we generated ADAM9 knockdown clones of MDA-MB-231 breast tumor cells using silencing RNAs that were tested for cell adhesion, proliferation, migration and invasion assays. In RNAi-mediated ADAM9 silenced MDA-MB-231 cells, the expression of ADAM9 was lower from the third to the sixth day after silencing and inhibited tumor cell invasion in matrigel by approximately 72% when compared to control cells, without affecting cell adhesion, proliferation or migration. In conclusion, the generation of MDA-MB-231 knockdown clones lacking ADAM9 expression inhibited tumor cell invasion in vitro, suggesting that ADAM9 is an important molecule in the processes of invasion and metastasis.
authors.affiliation: Departamento de Ciências Fisiológicas, Rodovia Washington Luís, Km 235, CEP 13565-905, São Carlos, SP, Brazil.
authors.forename: Kelli Cristina
authors.initials: KC
authors.lastname: Micocci
authors.forename: Ana Carolina Baptista Moreno
authors.initials: AC
authors.lastname: Martin
authors.forename: Cyntia de Freitas
authors.initials: Cde F
authors.lastname: Montenegro
authors.forename: Araceli Cristina
authors.initials: AC
authors.lastname: Durante
authors.forename: Normand
authors.initials: N
authors.lastname: Pouliot
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Cominetti
authors.forename: Heloisa Sobreiro
authors.initials: HS
authors.lastname: Selistre-de-Araujo
mesh_terms: D051722:ADAM Proteins / Q000235:genetics* / Q000378:metabolism; D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D008565:Membrane Proteins; D034741:RNA, Small Interfering; D051722:ADAM Proteins; C098339:ADAM9 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9