title: Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene-divinylbenzene beads.
abstract: Two immobilized preparations from Thermomyces lanuginosus lipase (TLL) were compared in the synthesis of butyl butyrate. The commercial Lipozyme TL-IM, and TLL immobilized on styrene-divinylbenzene beads (MCI-TLL) were tested in the esterification reaction using n-hexane as solvent. The variables temperature (30-60°C), substrate molar ratio (1:1 to 5:1), added water (0-1%), and biocatalyst content (3-40%) were evaluated in terms of initial reaction rate for each biocatalyst. SDS-PAGE analysis revealed that MCI-TLL had an immobilized enzymatic load twice as high as Lipozyme TL-IM, but with an activity 3-fold higher. MCI-TLL presented high initial reaction rates up to 1.0 M butyric acid, while Lipozyme TL-IM showed a decrease in its activity above 0.5 M. Moreover, MCI-TLL allowed a productivity of 14.5 mmol g(-1) h(-1), while Lipozyme TL-IM 3.2 mmol g(-1) h(-1), both by mass of biocatalyst.
authors.affiliation: Biocatalysis and Enzyme Technology Lab, Institute of Food Science and Technology, Federal University of Rio Grande do Sul State, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil.
authors.forename: Andréa B
authors.initials: AB
authors.lastname: Martins
authors.forename: John L R
authors.initials: JL
authors.lastname: Friedrich
authors.forename: Jhonnattas C
authors.initials: JC
authors.lastname: Cavalheiro
authors.forename: Cristina
authors.initials: C
authors.lastname: Garcia-Galan
authors.forename: Oveimar
authors.initials: O
authors.lastname: Barbosa
authors.forename: Marco A Z
authors.initials: MA
authors.lastname: Ayub
authors.forename: Roberto
authors.initials: R
authors.lastname: Fernandez-Lafuente
authors.forename: Rafael C
authors.initials: RC
authors.lastname: Rodrigues
mesh_terms: D001203:Ascomycota / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D055162:Biocatalysis / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D002087:Butyrates / Q000378:metabolism* / Q000494:pharmacology; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004951:Esterification / Q000187:drug effects; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008863:Microspheres; D020058:Styrene / Q000494:pharmacology*; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D014753:Vinyl Compounds / Q000494:pharmacology*; D014867:Water / Q000494:pharmacology
chemical_list: D002087:Butyrates; D014753:Vinyl Compounds; D014867:Water; C022793:n-butyl n-butyrate; D020058:Styrene; C093666:Lipozyme; D008049:Lipase; C004985:divinyl benzene