title: Establishment of a transgenic zebrafish EF1α:Kaede for monitoring cell proliferation during regeneration.
abstract: Zebrafish is considered as a versatile experimental animal for various research models from development to diseases. In this study, we report the development of transgenic zebrafish line named as Tg(EF1α:Kaede) that expresses translation elongation factor 1 subunit alpha (EF1α) promoter linked to a fluorescent protein (FP), Kaede for monitoring proliferating cells in during regeneration. It was revealed that about 1.4 kb 5'-flanking region of the EF1α was sufficient for its promoter activity. Expression of Kaede with a property of photo-conversion from green to red was detected in different embryonic stages as well as various organs such as brain, heart, pancreas, intestine, ovary, and fins of adult fish. Cell proliferation pattern during fin regeneration was monitored after amputation of Tg(EF1α:Kaede) caudal fin and results shown that this system is simple and efficient method for detecting proliferating cells during tissue regeneration. Developed Tg(EF1α:Kaede) line has potential to investigate the cell proliferation, regeneration, wound healing capacities after tissue damage and evaluate the therapeutic power of wound healing drugs.
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Hyun-Yi
authors.initials: HY
authors.lastname: Moon
authors.forename: Oc-Hee
authors.initials: OH
authors.lastname: Kim
authors.forename: Hyun-Taek
authors.initials: HT
authors.lastname: Kim
authors.forename: Jung-Hwa
authors.initials: JH
authors.lastname: Choi
authors.forename: Sang-Yeob
authors.initials: SY
authors.lastname: Yeo
authors.forename: Nam-Soon
authors.initials: NS
authors.lastname: Kim
authors.forename: Doo-Sang
authors.initials: DS
authors.lastname: Park
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Oh
authors.forename: Kwan-Hee
authors.initials: KH
authors.lastname: You
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000671:Amputation, Surgical; D058500:Animal Fins / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C513582:Kaede protein, Trachyphyllia geoffroyi; D008164:Luminescent Proteins; D020648:Peptide Elongation Factor 1; D029961:Zebrafish Proteins