title: Frequency of angiotensin II type 1 receptor gene polymorphism in Turkish acute stroke patients.
abstract: This study was performed in acute stroke patients in the Turkish population to determine the frequency of the A1166C polymorphism in the AT1 gene and to examine the role of this polymorphism in acute stroke development. In this study, 257 genomic DNA samples were analysed (from 206 acute stroke patients and 51 healthy individuals). Genomic DNA was prepared from peripheral blood using the salt-extraction method. The presence of the A1166C polymorphism in the AT1 gene was determined using the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge-coupled device (CCD) camera. In this study, the allele frequency at the A1166C position was 92% A and 8% C for control and 97% A and 3% C for patients. This difference in allele frequency between the control group and the patient group was not statistically significant. However, genotype and allele frequencies showed a significant difference (P < 0.001) in the control and the patient groups. The results of this study show no relationship between the A1166C polymorphism in the AT1 gene and acute stroke in the Turkish population.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir 26480, Turkey. hkurtayda@gmail.com
authors.forename: Kurt
authors.initials: K
authors.lastname: Hulyam
authors.forename: Bayramoglu
authors.initials: B
authors.lastname: Aysegul
authors.forename: Gunes Hasan
authors.initials: GH
authors.lastname: Veysi
authors.forename: Ozbabalik
authors.initials: O
authors.lastname: Demet
authors.forename: Degirmenci
authors.initials: D
authors.lastname: Irfan
authors.forename: Colak
authors.initials: C
authors.lastname: Ertugrul
authors.forename: Cosan Turgut
authors.initials: CT
authors.lastname: Didem
authors.forename: Bayram
authors.initials: B
authors.lastname: Banu
authors.forename: Dikmen
authors.initials: D
authors.lastname: Miris
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020521:Stroke / Q000235:genetics*; D014421:Turkey
chemical_list: D044140:Receptor, Angiotensin, Type 1