title: Chiayiivirga flava gen. nov., sp. nov., a novel bacterium of the family Xanthomonadaceae isolated from an agricultural soil, and emended description of the genus Dokdonella.
abstract: A novel Gram-reaction-negative, yellow-pigmented, aerobic, non-motile and rod-shaped bacterium designated strain CC-YHH031(T) was isolated from an agricultural soil collected at Chiayi County, Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CC-YHH031(T) formed a discrete monophyletic lineage in the family Xanthomonadaceae, sharing high pairwise sequence similarity of 93.5-95.2 and 94.8% with species of the genus Dokdonella (94.9% similarity to the type strain of the type species) and Aquimonas voraii GPTSA 20(T), respectively. The genomic DNA G+C content of strain CC-YHH031(T) was 68.6 ± 0.7 mol% and the predominant respiratory quinone was ubiquinone Q-8. Spermidine was the principal polyamine, with minor amounts of putrescine. Major fatty acids (>5% of total fatty acids) were iso-C(16:00, iso-C(15:0), C(16:1)ω7c and/or C(16:1)ω6c (summed feature 3), iso-C(17:1)ω9c, iso-C(14:0), iso-C(11:0) and iso-C(11:0) 3-OH. The polar lipid profile of strain CC-YHH031(T) included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids (APL1-2) and four unidentified phospholipids (PL1-4). Strain CC-YHH031(T) was distinguished particularly from the type species of the genus Dokdonella (Dokdonella koreensis) by the presence of major amounts of iso-C(14:0) and summed feature 3 and minor amounts of iso-C(17:0) and by the complete absence of anteiso-C(17:0), the presence of PL1-3 and APL1-2, the absence of APL3 and the presence of putrescine in the former. On the basis of distinguishing genotypic and phenotypic evidence, strain CC-YHH031(T) is proposed to represent a novel genus and species within the family Xanthomonadaceae, for which the name Chiayiivirga flava gen. nov., sp. nov. is proposed. The type strain of Chiayiivirga flava is CC-YHH031(T) ( =BCRC 80274(T) =DSM 24163(T)).
authors.affiliation: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Hsu
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taiwan, ROC.|Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Wei-An
authors.initials: WA
authors.lastname: Lai
authors.affiliation: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Shih-Yao
authors.initials: SY
authors.lastname: Lin
authors.affiliation: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Asif
authors.initials: A
authors.lastname: Hameed
authors.affiliation: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Mariyam
authors.initials: M
authors.lastname: Shahina
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taiwan, ROC.|Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Fo-Ting
authors.initials: FT
authors.lastname: Shen
authors.affiliation: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Zhi-Long
authors.initials: ZL
authors.lastname: Zhu
authors.affiliation: Department of Biotechnology, National Formosa University, Taiwan, ROC.
authors.forename: Li-Sen
authors.initials: LS
authors.lastname: Young
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taiwan, ROC.|Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013095:Spermidine / Q000032:analysis; D013624:Taiwan; D014451:Ubiquinone / Q000032:analysis; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8; D013095:Spermidine