title: Identification and Characterization of a High Vancomycin-Resistant Staphylococcus aureus Harboring VanA Gene Cluster Isolated from Diabetic Foot Ulcer.
abstract: OBJECTIVE
  Staphylococcus aureus is a common cause of human infection, and emergence of vancomycin-resistance S. aureus is a great concern for treatment of methicillin-resistant S. aureus,(MRSA) in recent years (MRSA). Here, we report the isolation of high-level VRSA.
  
  
  METHODS
  S. aureus was isolated from foot ulcer of a diabetic woman in Tehran, Iran. Antibiotic susceptibility was determined according to CLSI guidelines. VanA gene cluster PCR was carried out and PCR amplicon of vanA was sequenced.
  
  
  RESULTS
  S. aureus had high-level vancomycin-resistant (MIC 512 ≥ µg/ml). Patient's history revealed that VRSA isolate was acquired through community transmission. Only vanA, vanR and vanS genes were amplified in our isolate. Sequencing revealed that the vanA sequence had high similarity to the vanA sequence of Tn1546.
  
  
  CONCLUSIONS
  Although VRSA infection continues to be rare, isolation of community-acquired VRSA is a significant issue and it needs the efforts of public health authorities.
authors.affiliation: Research Centre of Gastroenterology and Liver Diseases (RCGLD) in Shahid Beheshti University, Tehran, Iran.
authors.forename: Anahita
authors.initials: A
authors.lastname: Dezfulian
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Aslani
authors.forename: Mahvash
authors.initials: M
authors.lastname: Oskoui
authors.forename: Parisa
authors.initials: P
authors.lastname: Farrokh
authors.forename: Masumeh
authors.initials: M
authors.lastname: Azimirad
authors.forename: Hossein
authors.initials: H
authors.lastname: Dabiri
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Salehian
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali