title: Diversity of pea-associated F. proliferatum and F. verticillioides populations revealed by FUM1 sequence analysis and fumonisin biosynthesis.
abstract: Fusarium proliferatum and F. verticillioides are considered as minor pathogens of pea (Pisum sativum L.). Both species can survive in seed material without visible disease symptoms, but still contaminating it with fumonisins. Two populations of pea-derived F. proliferatum and F. verticillioides strains were subjected to FUM1 sequence divergence analysis, forming a distinct group when compared to the collection strains originating from different host species. Furthermore, the mycotoxigenic abilities of those strains were evaluated on the basis of in planta and in vitro fumonisin biosynthesis. No differences were observed in fumonisin B (FB) levels measured in pea seeds (maximum level reached 1.5 μg g(-1)); however, in rice cultures, the majority of F. proliferatum genotypes produced higher amounts of FB1-FB3 than F. verticillioides strains.
authors.affiliation: Department of Chemistry, Poznan University of Life Sciences, Poznań, Poland. agat@up.poznan.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Waśkiewicz
authors.forename: Lukasz
authors.initials: L
authors.lastname: Stępień
authors.forename: Karolina
authors.initials: K
authors.lastname: Wilman
authors.forename: Piotr
authors.initials: P
authors.lastname: Kachlicki
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D037341:Fumonisins / Q000032:analysis / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis; D005670:Fusarium / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D018532:Pisum sativum / Q000382:microbiology*; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D061805:Transcription Elongation, Genetic
chemical_list: D037341:Fumonisins; D005656:Fungal Proteins; C056934:fumonisin B2; C073063:fumonisin B3; C056933:fumonisin B1; D048630:Polyketide Synthases