title: Dietary exposure of juvenile female mice to polyhalogenated seafood contaminants (HBCD, BDE-47, PCB-153, TCDD): comparative assessment of effects in potential target tissues.
abstract: Fish represents source of nutrients and major dietary vehicle of lipophilic persistent contaminants. The study compared the effects of two legacy and two emerging fish pollutants (Hexabromocyclododecane HBCD; 2,2',4,4'-Tetrabromodiphenyl ether BDE-47; 2,2',4,4',5,5'-Hexachlorobiphenyl PCB-153; 2,3,7,8-Tetrachlorodibenzo-p-doxin TCDD) in juvenile female mice exposed through a salmon based rodent diet for 28 days (dietary doses: HBCD 199 mg/kg bw/day; BDE-47 450 μg/kg bw/day; PCB-153 195 μg/kg bw/day; TCDD 90 ng/kg bw/day). Dose levels were comparable to previously reported developmental Lowest Observed Adverse Effect Levels. None of the treatments elicited signs of overt toxicity, but HBCD increased relative liver weight. All compounds caused changes in liver, thymus and thyroid; spleen was affected by BDE-47 and PCB-153; no effects were seen in uterus and adrenals. Strongest effects in thyroid follicles were elicited by PCB-153, in thymus and liver by BDE-47. HBCD and BDE-47 induced liver fatty changes, but appeared to be less potent in the other tissues. HBCD, BDE-47 and TCDD increased serum testosterone levels and the testosterone/estradiol ratio, suggesting a potential involvement of pathways related to sex steroid biosynthesis and/or metabolism. The results support the role of toxicological studies on juvenile rodents in the hazard characterization of chemicals, due to endocrine and/or immune effects.
authors.affiliation: Food and Veterinary Toxicology Unit, Veterinary Public Health and Food Safety Dept., Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy. francesca.maranghi@iss.it
authors.forename: F
authors.initials: F
authors.lastname: Maranghi
authors.forename: R
authors.initials: R
authors.lastname: Tassinari
authors.forename: G
authors.initials: G
authors.lastname: Moracci
authors.forename: I
authors.initials: I
authors.lastname: Altieri
authors.forename: J D
authors.initials: JD
authors.lastname: Rasinger
authors.forename: T S
authors.initials: TS
authors.lastname: Carroll
authors.forename: C
authors.initials: C
authors.lastname: Hogstrand
authors.forename: A-K
authors.initials: AK
authors.lastname: Lundebye
authors.forename: A
authors.initials: A
authors.lastname: Mantovani
mesh_terms: D000818:Animals; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D005399:Fishes; D005506:Food Contamination; D012739:Gonadal Steroid Hormones / Q000097:blood; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D017747:Seafood; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism
chemical_list: D012739:Gonadal Steroid Hormones; D055768:Halogenated Diphenyl Ethers; D006842:Hydrocarbons, Brominated; D000072317:Polychlorinated Dibenzodioxins; C511295:2,2',4,4'-tetrabromodiphenyl ether; C089796:hexabromocyclododecane; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl