title: Analysis of ascorbic acid biosynthesis using a simple transient gene expression system in tomato fruit protoplasts.
abstract: We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clarify the function of AsA biosynthetic enzymes and AsA roles in tomato fruit.
authors.affiliation: Graduate School of Biosphere Sciences, Hiroshima University, Higashi-hiroshima, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Sakamoto
authors.forename: Yukichi
authors.initials: Y
authors.lastname: Fujikawa
authors.forename: Muneharu
authors.initials: M
authors.lastname: Esaka
mesh_terms: D001205:Ascorbic Acid / Q000096:biosynthesis*; D005638:Fruit / Q000166:cytology*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D018551:Solanum lycopersicum / Q000166:cytology / Q000235:genetics*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D011523:Protoplasts / Q000378:metabolism*; D013997:Time Factors; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics
chemical_list: C069910:galactose-1-phosphatase; D010744:Phosphoric Monoester Hydrolases; D001205:Ascorbic Acid