title: Expression of microRNA-30c and its target genes in human osteoblastic cells by nano-bioglass ceramic-treatment.
abstract: Osteoblast differentiation is tightly regulated by post transcriptional regulators such as microRNAs (miRNAs). Several bioactive materials including nano-bioglass ceramic particles (nBGC) influence differentiation of the osteoblasts, but the molecular mechanisms of nBGC-stimulation of osteoblast differentiation via miRNAs are not yet determined. In this study, we identified that nBGC-treatment stimulated miR-30c expression in human osteoblastic cells (MG63). The bioinformatics tools identified its regulatory network, molecular function, biological processes and its target genes involved in negative regulation of osteoblast differentiation. TGIF2 and HDAC4 were found to be its putative target genes and their expression was down regulated by nBGC-treatment in MG63 cells. Thus, this study advances our understanding of nBGC action on bone cells and supports utilization of nBGC in bone tissue engineering.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Moorthi
authors.forename: S
authors.initials: S
authors.lastname: Vimalraj
authors.forename: C
authors.initials: C
authors.lastname: Avani
authors.forename: Zhiming
authors.initials: Z
authors.lastname: He
authors.forename: Nicola C
authors.initials: NC
authors.lastname: Partridge
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002516:Ceramics / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks / Q000187:drug effects / Q000235:genetics; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: C022107:Bioglass; D018398:Homeodomain Proteins; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; C414585:TGIF2 protein, human; C400300:HDAC4 protein, human; D006655:Histone Deacetylases