title: Isolation of hair follicle bulge stem cells from YFP-expressing reporter mice.
abstract: In this article we provide a method to isolate hair follicle stem cells that have undergone targeted gene inactivation. The mice from which these cells are isolated are bred into a Rosa26-yellow fluorescent protein (YFP) reporter background, which results in YFP expression in the targeted stem cell population. These cells are isolated and purified by fluorescence-activated cell sorting, using epidermal stem cell-specific markers in conjunction with YFP fluorescence. The purified cells can be used for gene expression studies, clonogenic experiments, and biological assays, such as viability and capacity for directional migration.
authors.affiliation: Department of Physiology and Pharmacology, Child Health Research Institute, University of Western Ontario, London, ON, Canada.
authors.forename: Kerry-Ann
authors.initials: KA
authors.lastname: Nakrieko
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Irvine
authors.forename: Lina
authors.initials: L
authors.lastname: Dagnino
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005260:Female; D005434:Flow Cytometry; D018859:Hair Follicle / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D013234:Stem Cells / Q000166:cytology*
chemical_list: D008164:Luminescent Proteins