title: Isolation of mesenchymal stem cells from human dermis.
abstract: Recent studies revealed that mammalian dermis contains multipotent stem cells such as skin-derived precursors (SKPs). SKPs grow in suspension as spheres. In contrast, mesenchymal stem cells (MSCs) are adherent fibroblastic cells. Here, we describe the procedure to isolate MSCs under low-serum culture conditions. In addition, we explain the method to collect MSCs using magnetic cell sorting.
authors.affiliation: Shiseido Innovative Science Research and Development Center, Yokohama, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Soma
authors.forename: Jiro
authors.initials: J
authors.lastname: Kishimoto
authors.forename: David
authors.initials: D
authors.lastname: Fisher
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D012535:Scalp / Q000166:cytology