title: Establishment of an acute rejection model by transplanting both renal grafts into two different rats using a modified method of ureterovesical anastomosis.
abstract: OBJECTIVE
  To establish an acute rejection model after kidney transplantation in the rat using a modified method of ureterovesical anastomosis.
  
  
  METHODS
  Thirty-nine Wistar rat donors, were transplanted into 70 male SD rats. Wistar rats (group 1; n = 18) underwent harvest of both kidneys, cold perfusion, and transplantation into 36 SD rats. Wistar rats (group 2; n = 18) underwent left kidney harvest, cold perfusion and transplantation into 18 SD rats. Groups 1 and 2 did not receive immunosuppression after transplantation. Six kidneys were harvested from 3 Wistar rats (group 3), were transplanted into 6 SD rats that were treated with CsA (5 mg/kg per day) postoperatively, and humanely killed at 21 days. There were 10 SD in sham operated rats (group 4). The renal allograft vein was end-to-end anastomosed to the recipient renal vein using an epidural catheter. The renal allograft was anastomosed end-to-side to the recipient abdominal aorta with an abdominal aortic flap. The renal allograft ureterovesical flap was directly inserted into the recipient bladder, and attached by 4-5 interrupted sutures. The recipient's right kidney vessels were ligated at 3 days postoperatively.
  
  
  RESULTS
  The success rates were 91.7% and 88.9% in groups 1 and 2, respectively. Except for the time for removal of the renal allografts, the operative durations and warm ischemia times differed insignificantly between both groups (P > .05). Blood creatinine levels increased significantly after kidney transplantation in groups 1 and 2 compared with the sham operated and CsA-treated cohorts (P < .01), but insignificantly between groups 1 and 2 (P > .05).
  
  
  CONCLUSIONS
  A dual renal allograft model was established in the rat using a modified ureterovesical anastomosis. The technique can be reproduced reliably, reducing costs and shorten using overall operative duration.
authors.affiliation: Department of Urology, General Hospital of Chengdu Military Command, Chengdu, China. lishadan@hotmail.com
authors.forename: S D
authors.initials: SD
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: P
authors.initials: P
authors.lastname: Ye
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: Q T
authors.initials: QT
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: W G
authors.initials: WG
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Zhou
authors.forename: X W
authors.initials: XW
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Liang
authors.forename: Y G
authors.initials: YG
authors.lastname: Zhao
authors.forename: Q W
authors.initials: QW
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.forename: X C
authors.initials: XC
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: Q S
authors.initials: QS
authors.lastname: Fan
mesh_terms: D000208:Acute Disease; D000714:Anastomosis, Surgical; D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D004195:Disease Models, Animal; D006084:Graft Rejection / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D016030:Kidney Transplantation / Q000009:adverse effects / Q000276:immunology / Q000379:methods*; D008026:Ligation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012082:Renal Veins / Q000601:surgery; D013536:Suture Techniques; D013997:Time Factors; D014513:Ureter / Q000601:surgery; D001743:Urinary Bladder / Q000601:surgery
chemical_list: D007166:Immunosuppressive Agents