title: p-Coumaric acid modulates glucose and lipid metabolism via AMP-activated protein kinase in L6 skeletal muscle cells.
abstract: p-Coumaric acid (3-[4-hydroxyphenyl]-2-propenoic acid) is a ubiquitous plant metabolite with antioxidant, anti-inflammatory, and anticancer properties. In this study, we examined whether p-coumaric acid modulates glucose and lipid metabolism via AMP-activated protein kinase (AMPK) in L6 skeletal muscle cells. p-Coumaric acid increased the phosphorylation of AMPK in a dose-dependent manner in differentiated L6 skeletal muscle cells. It also increased the phosphorylation of acetyl-CoA carboxylase (ACC) and the expression of CPT-1 mRNA and PPARα, suggesting that it promotes the β-oxidation of fatty acids. Also, it suppressed oleic acid-induced triglyceride accumulation, and enhanced 2-NBDG uptake in differentiated L6 muscle cells. Pretreatment with compound C inhibited AMPK activation, reduced ACC phosphorylation and 2-NBDG uptake, and increased triglyceride accumulation. However, p-coumaric acid counterbalanced the inhibitory effects of compound C. Taken together, these results suggest that p-coumaric acid modulates glucose and lipid metabolism via AMPK activation in L6 skeletal muscle cells and that it has potentially beneficial effects in improving or treating metabolic disorders.
authors.affiliation: Department of Biology, Jeju National University, Jejusi, Jeju 690-756, Republic of Korea.
authors.forename: Seon-A
authors.initials: SA
authors.lastname: Yoon
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Kang
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Shin
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Hee-Chul
authors.initials: HC
authors.lastname: Ko
authors.forename: Se-Jae
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002460:Cell Line; D003373:Coumaric Acids / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology; D010084:Oxidation-Reduction / Q000187:drug effects; D047493:PPAR alpha / Q000378:metabolism; D011422:Propionates; D051381:Rats
chemical_list: D003373:Coumaric Acids; D005227:Fatty Acids; D047493:PPAR alpha; D011422:Propionates; D002334:Carnitine O-Palmitoyltransferase; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase; C495469:p-coumaric acid; D005947:Glucose