title: Comparison of computed tomography- and optical image-based assessment of liposome distribution.
abstract: The use of multimodal imaging as a tool to assess the in vivo pharmacokinetics and biodistribution of nanocarriers is important in understanding the nature of their in vivo transport. The current study reports the development of a nano-sized liposomal computed tomographic (CT)/optical imaging probe carrying iohexol and Cy5.5 and its use in micro-CT and optical imaging to quantitatively assess the whole-body (macroscopic), intratumoral, and microscopic distribution over a period of 8 days. These multimodal liposomes have a vascular half-life of 30.3 ± 8.9 hours in mice bearing subcutaneous H520 non-small cell lung cancer tumors, with the maximum liposome accumulation in tumor achieved 48 hours postinjection. The in vivo liposome distribution and stability were quantitatively assessed using both micro-CT and fluorescence molecular tomography. The combination of CT and optical imaging enables visualization of the liposomes at the whole-body, tumor, and cellular scales with high sensitivity. Such noninvasive tracking of therapeutic vehicles at the macro- and microscale is important for informed and rational development of novel nanocarrier systems.
authors.affiliation: Leslie Dan Faculty of Pharmacy, Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.
authors.forename: Huang
authors.initials: H
authors.lastname: Huang
authors.forename: Michael
authors.initials: M
authors.lastname: Dunne
authors.forename: John
authors.initials: J
authors.lastname: Lo
authors.forename: David A
authors.initials: DA
authors.lastname: Jaffray
authors.forename: Christine
authors.initials: C
authors.lastname: Allen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional; D007472:Iohexol; D008081:Liposomes / Q000008:administration & dosage / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D008081:Liposomes; D007472:Iohexol