title: Selection of stable reference genes in heat stressed peripheral blood mononuclear cells of tropically adapted Indian cattle and buffaloes.
abstract: Eleven reference genes from different functional categories were assessed for their stable expression pattern in heat stressed PBMCs across Indian buffalo and cattle using GeNorm, NormFinder and BestKeeper algorithms. As the first report, we suggest B2M, RPS9 and RPS15a as suitable reference genes for accurate normalization of PBMC transcript data while the ACTB gene is not recommended.
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, Haryana 132001, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kishore
authors.forename: Monika
authors.initials: M
authors.lastname: Sodhi
authors.forename: Keviletsu
authors.initials: K
authors.lastname: Khate
authors.forename: Neha
authors.initials: N
authors.lastname: Kapila
authors.forename: Parvesh
authors.initials: P
authors.lastname: Kumari
authors.forename: Manishi
authors.initials: M
authors.lastname: Mukesh
mesh_terms: D000465:Algorithms; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D020869:Gene Expression Profiling; D006360:Heat-Shock Proteins / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014329:Tropical Climate
chemical_list: D006360:Heat-Shock Proteins