title: Overexpression of peroxiredoxin 2 in pterygium. A proteomic approach.
abstract: Pterygium is one of the most frequent pathologies in ophthalmology, and is a benign, fibrovascular lesion originating from the bulbar conjunctiva. It is composed of an epithelium and highly vascular, subepithelial, loose connective tissue. The etiology of pterygium is not clearly understood; the most widely recognized originating factor is ultraviolet radiation. It has been proposed that pterygium and neoplasia have common features, raising the possibility that pterygium is a neoplastic-like growth disorder. In this study, proteomic analysis was performed to show that peroxiredoxin 2 is overexpressed in pterygia compared to healthy conjunctivas. Twelve pterygium specimens were obtained together with healthy conjunctival tissue from the same eyes. Total proteins of pterygia and healthy conjunctivas were analyzed in SDS-PAGE. This analysis showed protein bands expressed exclusively in pterygium samples at the range of 20-25 kDa. After this, 2D electrophoresis was performed for the separation of total proteins; differential spots expressed in pterygium were excised and sequenced. Mass spectrometry (MS) data were searched in the NCBInr and EST databases using the MASCOT program. The spot was identified as peroxiredoxin 2. Real-time PCR, western blot and immunohistochemistry showed that peroxiredoxin 2 was increased in pterygium compared to healthy conjunctiva. Although, these results suggest that overexpression of peroxiredoxin 2 in pterygium could protect the cell against oxidative stress-induced apoptosis, further studies are required to establish the functional role of peroxiredoxin 2 in pterygium to determine its role in peroxidation and apoptosis in this pathology.
authors.affiliation: Microbiology and Ocular Proteomics, Research Unit, Institute of Ophthalmology Fundación de Asistencia Privada Conde de Valenciana, Chimalpopoca, 14 Colonia Obrera, 06800 México City, Mexico. vbautistal@institutodeoftalmologia.org
authors.forename: V M
authors.initials: VM
authors.lastname: Bautista-de Lucio
authors.forename: N L
authors.initials: NL
authors.lastname: López-Espinosa
authors.forename: A
authors.initials: A
authors.lastname: Robles-Contreras
authors.forename: H J
authors.initials: HJ
authors.lastname: Pérez-Cano
authors.forename: H
authors.initials: H
authors.lastname: Mejía-López
authors.forename: G
authors.initials: G
authors.lastname: Mendoza
authors.forename: M C
authors.initials: MC
authors.lastname: Jiménez-Martínez
authors.forename: Y
authors.initials: Y
authors.lastname: Garfias
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015153:Blotting, Western; D003228:Conjunctiva / Q000201:enzymology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D011625:Pterygium / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005136:Eye Proteins; D012333:RNA, Messenger; C086065:PRDX2 protein, human; D054464:Peroxiredoxins