title: Effects of bilberry (Vaccinium myrtillus) in combination with lactic acid bacteria on intestinal oxidative stress induced by ischemia-reperfusion in mouse.
abstract: Intestinal ischemia-reperfusion (I/R) results in oxidative stress, inflammation, and tissue injuries. The present study investigates the antioxidative and anti-inflammatory effects of a dietary supplement of bilberry, either alone or in combination with Lactobacillus plantarum RESO56, L. plantarum HEAL19, or Pediococcus acidilactici JAM046, in an I/R-induced model for oxidative stress in mice. A bilberry diet without addition of bacteria significantly decreased both lipid peroxidation (p = 0.001) and mucosal injury in the ileum. Of 14 anthocyanins identified in bilberry, anthocyanin arabinosides were the most resistant to absorption and microbial degradation in the intestines. Cyanidin-3-glucoside and delphinidin-3-glucoside seemed to be mostly absorbed in the stomach and upper part of the small intestine, while malvidin-3-galactoside, peonidin-3-glucoside, peonidin-3-galactoside, and petunidin-3-galactoside seemed to be digested by the microbiota in the cecum. Bilberry strongly influenced the composition of the cecal microbiota. In conclusion, a food supplement of bilberry protected small intestine against oxidative stress and inflammation induced by ischemia-reperfusion.
authors.affiliation: Food Hygiene, Department of Food Technology, Engineering and Nutrition, Lund University, Lund, Sweden. Maja.Jakesevic@gmail.com
authors.forename: Maja
authors.initials: M
authors.lastname: Jakesevic
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Kjersti
authors.initials: K
authors.lastname: Aaby
authors.forename: Bengt
authors.initials: B
authors.lastname: Jeppsson
authors.forename: Siv
authors.initials: S
authors.lastname: Ahrné
authors.forename: Göran
authors.initials: G
authors.lastname: Molin
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000627:therapeutic use*; D000975:Antioxidants / Q000737:chemistry / Q000627:therapeutic use*; D019587:Dietary Supplements / Q000032:analysis / Q000382:microbiology; D005638:Fruit / Q000737:chemistry; D007413:Intestinal Mucosa / Q000098:blood supply / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007422:Intestines / Q000098:blood supply* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007511:Ischemia / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress; D010376:Pediococcus / Q000254:growth & development / Q000302:isolation & purification; D011897:Random Allocation; D015427:Reperfusion Injury / Q000517:prevention & control*; D029800:Vaccinium myrtillus / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants