title: Steroidal glycosides from the bulbs of Fritillaria meleagris and their cytotoxic activities.
abstract: Steroidal glycosides (1-18), including 10 new compounds (1-10), were isolated from the bulbs of Fritillaria meleagris (Liliaceae). The structures of the new compounds were determined by two-dimensional (2D) NMR analysis, and by hydrolytic cleavage followed by spectroscopic and chromatographic analysis. The isolated compounds and their aglycones were evaluated for cytotoxic activity against HL-60 human promyelocytic leukemia cells and A549 human lung adenocarcinoma cells. Morphological observation and flow cytometry analysis showed that 5β-spirostanol glycoside (2) and a cholestane derivative (17a) induced apoptotic cell death in HL-60 cells through different mechanisms of action. Furthermore, the (22R)-spirosolanol glycoside (11) selectively induced apoptosis in A549 cells without affecting the caspase-3 activity level.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. matsuoy@toyaku.ac.jp
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shinoda
authors.forename: Aina
authors.initials: A
authors.lastname: Nakamaru
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mimaki
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D005434:Flow Cytometry; D031410:Fritillaria; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D015394:Molecular Structure; D013256:Steroids / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D006027:Glycosides; D013256:Steroids; D053148:Caspase 3