title: Paradoxical sleep deprivation potentiates epilepsy induced by homocysteine thiolactone in adult rats.
abstract: There is an intriguing and still poorly understood relationship between sleep deprivation and epilepsy. It has recently been shown that paradoxical sleep deprivation decreases levels of homocysteine, an amino acid involved together with its thiolactone metabolite in epileptogenesis. The aim of the present study was to investigate the effects of paradoxical sleep deprivation on homocysteine thiolactone (H)-induced seizures in rats, a model of generalized seizures. Selective deprivation of paradoxical sleep in adult male Wistar rats was achieved by the platform method. Animals with implanted electrodes for electroencephalogram (EEG) registration were assigned to appropriate experimental conditions (dry cage for control, large platform for stress control and small platform for paradoxical sleep deprivation) and 72 h later were intraperitoneally treated with either H (5.5 mmol/kg) or saline (0.9% NaCl). This study showed that paradoxical sleep deprivation increased the incidence and number of H-induced seizure episodes, shortened latency time to seizures and led to significant rates of lethality after H administration, but without effect on the seizure severity. Paradoxical sleep deprivation increased the number and duration of spikes-and-wave discharges, while decreased latency to its appearance in EEG. Judging by the behavioral and EEG findings, it could be concluded that paradoxical sleep deprivation can provoke the expression of factors that can potentiate H-induced seizures in adult rats.
authors.affiliation: Laboratory of Neurophysiology, Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, Višegradska 26/II, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Hrncić
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rasić-Marković
authors.forename: Jelica
authors.initials: J
authors.lastname: Bjekić-Macut
authors.forename: Veselinka
authors.initials: V
authors.lastname: Susić
authors.forename: Dragan
authors.initials: D
authors.lastname: Djuric
authors.forename: Olivera
authors.initials: O
authors.lastname: Stanojlović
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004569:Electroencephalography; D004827:Epilepsy / Q000139:chemically induced*; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000150:complications*; D012895:Sleep, REM
chemical_list: D006710:Homocysteine; C007957:homocysteine thiolactone