title: Effect of thermal stress on MICA/B induction in a human liposarcoma cell line.
abstract: BACKGROUND
  A possible mechanism by which hyperthermia enhances tumor immunogenicity is the induction of NKG2D ligands on tumor cells. Although the expression of MHC class I chain-related protein A and B (MICA/B) has previously been reported in different carcinomas, there is no information about MICA/B expression in liposarcomas.
  
  
  OBJECTIVE
  To investigate MICA/B induction in a human liposarcoma cell line (SW-872) after thermotherapy.
  
  
  METHODS
  SW-872 and HeLa cell lines were subjected to thermal stress for 1 h at 42, 44 and 46C, and after 2, 4 and 6 h of incubation at 37C, MICA/B expression was assessed at the mRNA and protein levels.
  
  
  RESULTS
  Despite high levels of MICA/B transcripts in SW-872 cells at baseline, the expression of these genes decreased significantly at both the mRNA and protein levels after almost all thermal treatments.
  
  
  CONCLUSIONS
  Our data conclude that thermotherapy under 42-46 C had no effect on MICA/B induction on SW-872 liposarcoma cell line but the effects of fever-range temperatures remain to be tested on this cell line.
authors.affiliation: Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran, farjadsh@sums.ac.ir.
authors.forename: Shirin
authors.initials: S
authors.lastname: Farjadian
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Rahimifar
authors.forename: Nasrollah
authors.initials: N
authors.lastname: Erfani
authors.forename: Ramin
authors.initials: R
authors.lastname: Lotfi
mesh_terms: D003602:Cytotoxicity, Immunologic; D005334:Fever / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006979:Hyperthermia, Induced; D007694:Killer Cells, Natural / Q000276:immunology*; D008080:Liposarcoma / Q000276:immunology* / Q000628:therapy*; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000378:metabolism; D013312:Stress, Physiological / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; C527085:KLRK1 protein, human; C099187:MHC class I-related chain A; D055655:NK Cell Lectin-Like Receptor Subfamily K