title: Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability.
abstract: The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Kunova
authors.forename: Kamil
authors.initials: K
authors.lastname: Matulka
authors.forename: Livia
authors.initials: L
authors.lastname: Eiselleova
authors.forename: Anton
authors.initials: A
authors.lastname: Salykin
authors.forename: Iva
authors.initials: I
authors.lastname: Kubikova
authors.forename: Sergiy
authors.initials: S
authors.lastname: Kyrylenko
authors.forename: Ales
authors.initials: A
authors.lastname: Hampl
authors.forename: Petr
authors.initials: P
authors.lastname: Dvorak
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D061252:Feeder Cells / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D013724:Teratoma / Q000473:pathology
chemical_list: D015415:Biomarkers