title: Long-term, inducible gene loss-of-function in the chicken embryo.
abstract: The use of shRNAmir to down-regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation-mediated transgenesis, the down-regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline-inducible, miR30-based shRNA plasmid with the Tol2 genomic integration system, we are now able to down-regulate the expression of any gene of interest at defined stage of chicken development.
authors.affiliation: EMBL Australia, Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton, Victoria, 3800, Australia. olivier.serralbo@monash.edu
authors.forename: Olivier
authors.initials: O
authors.lastname: Serralbo
authors.forename: Cyril A
authors.initials: CA
authors.lastname: Picard
authors.forename: Christophe
authors.initials: C
authors.lastname: Marcelle
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D015536:Down-Regulation; D004318:Doxycycline / Q000494:pharmacology; D018274:Electroporation; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics; D016254:Mutagenesis, Insertional; D009928:Organ Specificity / Q000235:genetics; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012045:Regulatory Sequences, Nucleic Acid / Q000187:drug effects
chemical_list: D004251:DNA Transposable Elements; D035683:MicroRNAs; D051763:PAX7 Transcription Factor; D004318:Doxycycline