title: The methylation status of the DDX43 promoter in Chinese patients with chronic myeloid leukemia.
abstract: Aberrant DNA methylation is a common epigenetic alteration and an important feature in human cancers. The DEAD box polypeptide 43 (DDX43) has been found to be overexpressed in various solid tumors and some hematologic malignancies. In the present study, we investigated the methylation status of the DDX43 promoter in 87 Chinese patients with chronic myeloid leukemia (CML) using real-time quantitative methylation-specific polymerase chain reaction and examined the DDX43 transcript in 35 patients using real-time quantitative polymerase chain reaction. DDX43 promoter hypomethylation was observed in 22 (25.3%) CML patients. No significant correlation was found between the hypomethylation of the DDX43 promoter with the age, sex, white blood cell counts, hemoglobin concentration, platelet counts, and chromosomal abnormalities of CML patients (p>0.05). The frequency of DDX43 hypomethylation in patients in the chronic phase, in the accelerated phase, and in blast crisis was 23.4% (15/64), 25.0% (2/8), and 33.3% (5/15), respectively (p>0.05). There was a significant correlation between DDX43 hypomethylation and DDX43 transcript (r=0.469, p=0.004). Our data suggest that hypomethylation of the DDX43 promoter may be an early and frequent molecular event in the development of CML in Chinese patients.
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Deng
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Chen
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Ma
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D009363:Neoplasm Proteins; C412324:DDX43 protein, human; D053487:DEAD-box RNA Helicases