title: RNA-guided genome editing à la carte.
abstract: Two recent papers in Science illustrate how the prokaryotic CRISPR-Cas immune system machinery, which typically targets invasive genetic elements such as viruses and plasmids, can be converted into a sophisticated molecular tool for next-generation human genome editing. The versatile Cas9 RNA-guided endonuclease can be readily reprogrammed using customizable small RNAs for sequence-specific single- or double-stranded DNA cleavage.
authors.affiliation: DuPont Nutrition and Health, Dangé-Saint-Romain, 86220, France. philippe.horvath@dupont.com
authors.forename: Philippe
authors.initials: P
authors.lastname: Horvath
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Barrangou
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004720:Endonucleases; D005818:Genetic Engineering; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D017393:RNA Editing / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D014780:Viruses / Q000235:genetics; D058727:RNA, Small Untranslated
chemical_list: D034741:RNA, Small Interfering; D012313:RNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D058727:RNA, Small Untranslated