title: Utility of serum DNA and pyrosequencing for the detection of EGFR mutations in non-small cell lung cancer.
abstract: Mutations in the EGFR gene are critical determinants of treatment with EGFR tyrosine kinase inhibitors (TKIs) for non-small cell lung cancer (NSCLC) patients. DNA isolation from tumor samples usually requires surgery; therefore, we wanted to isolate DNA from circulating tumor cells by using the serum of NSCLC patients. This protocol was recently published. DNA was isolated from the serum of 52 Turkish NSCLC patients and their EGFR mutation status was examined by pyrosequencing. EGFR mutations were detected in 25 of the 52 patients (48.1%): 17 patients with delE746-A750, 2 with delE747-A750insP, and 6 with L858R. All mutations detected by pyrosequencing were confirmed by dideoxy sequencing, and the presence of the same mutations in the tumors was verified by using paraffin embedded tissues of all the patients. Mutations were detected more frequently in adenocarcinomas (24 of 36, 66.7%) than in squamous cell carcinomas (1 of 16, 6.3%) (P<0.001). These results confirm the utility of serum DNA and pyrosequencing for the detection of EGFR mutations in patients with advanced NSCLC.
authors.affiliation: Department of Medical Biology, School of Medicine, Pamukkale University, Denizli, Turkey. hakca@pau.edu.tr
authors.forename: Hakan
authors.initials: H
authors.lastname: Akca
authors.forename: Aydın
authors.initials: A
authors.lastname: Demiray
authors.forename: Arzu
authors.initials: A
authors.lastname: Yaren
authors.forename: Ferda
authors.initials: F
authors.lastname: Bir
authors.forename: Aylin
authors.initials: A
authors.lastname: Koseler
authors.forename: Reika
authors.initials: R
authors.lastname: Iwakawa
authors.forename: Gulseren
authors.initials: G
authors.lastname: Bagci
authors.forename: Jun
authors.initials: J
authors.lastname: Yokota
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000368:Aged; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D006801:Humans; D016015:Logistic Models; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009154:Mutation; D059645:Mutation Rate; D020125:Mutation, Missense; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods; D017384:Sequence Deletion
chemical_list: D004273:DNA, Neoplasm; D066246:ErbB Receptors