title: [Effect of continuous cropping and soil treatment on rhizosphere fungal community of Panax quinquefolium].
abstract: OBJECTIVE
  To analyze the effects of continuous cropping and soil treatment on rhizosphere fungal community of Panax quinquefolium, and the correlation between rhizosphere fungal community and growth of P. quinquefolium.
  
  
  METHODS
  Field plot trail of continuous cultivated P. quinquefolium was conducted, meanwhile continuous cropping soil was treated by organic carbon fertilizer plus methylene dithiocyanate and Kingbo respectively. The rate of seedling survival, root disease index and root weight were investigated. Dilution plating was used to analyze the rhizosphere fungal community, multiple comparisons including H, M(a) and J were performed between rhizosphere fungal community and growth of P. quinquefolium.
  
  
  RESULTS
  As to P. quinquefolium planted in continuous soil, the rate of seedling survival, root weight decreased, root disease index increased remarkably (P<0.05). Meanwhile the fungal counts decreased 50% -63% , the diversity index (H') decreased 39%-43%, while the ratio of Penicillium and Aspergillus increased. There was a significant negative correlation between rhizosphere fungal diversity and P. quinquefolium root disease index (r = -0.970, P=0.006). Organic carbon fertilizer plus methylene dithiocyanate treatment could improve the rate of seedling survival and root weight, and could partly increase the rhizosphere fungal diversity.
  
  
  CONCLUSIONS
  In continuous cropping soil of P. quinquefolium, the rhizosphere fungal counts and diversity index decreased, meanwhile the fungal community composition changed. Organic carbon fertilizer plus methylene dithiocyanate treatment could improve the growth of continuous cultivated P. quinquefolium.
authors.affiliation: The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Zhang
authors.forename: Jia-Xue
authors.initials: JX
authors.lastname: Yang
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Jiao
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Gao
mesh_terms: D000383:Agriculture / Q000379:methods*; D044822:Biodiversity; D005308:Fertilizers / Q000032:analysis; D005658:Fungi / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D005894:Panax / Q000254:growth & development / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D005308:Fertilizers