title: RP105 involved in activation of mouse macrophages via TLR2 and TLR4 signaling.
abstract: RP105 is a member of the toll-like receptor family of proteins that transmits an activation signal in B cells, playing a role in regulation of B cell growth and death; in macrophages and dendritic cells, RP105 is a specific inhibitor of TLR4 signaling. RP105 is uniquely important for regulating TLR4-dependent signaling. It also proved that RP105 is closely related to TLR2 in macrophage activation by Mycobacterium tuberculosis lipoproteins. The aim of our study is to investigate the role of RP105 in mouse macrophages activation of TLR4 and TLR2 signaling by lipopolysaccharides (LPS) and Pam3CysSerLys4 (Pam3CSK4) alone or in combination, and the interaction between TLR2 and TLR4 signaling through RP105. Our results indicate that besides exhibiting negative regulation of TNF-α and IL12-p40 secretion in macrophage activated by LPS, RP105 is also involved in macrophages activation by Pam3CSK4 through TLR2 signaling and exhibited regulation to IL-10 and RANTES production by mouse peritoneal macrophage activated by Pam3CSK4. In macrophages activation by LPS and Pam3CSK4 in combination, TLR2 signaling can overcome RP105-mediated regulation of TLR4 signaling. Thus, our data demonstrate that not only TLR4 signaling, but also RP105 appears to be an essential accessory for immune responses through TLR2 signaling. The function of TLR2 and TLR4 in response to TLR ligands could be associated with each other by RP105. These results can help us understanding the unique role of RP105 in macrophages response to TLR ligands.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Naisheng
authors.initials: N
authors.lastname: Zhang
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Fu
authors.forename: Shuang
authors.initials: S
authors.lastname: Feng
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.forename: Yongguo
authors.initials: Y
authors.lastname: Cao
authors.forename: Depeng
authors.initials: D
authors.lastname: Li
authors.forename: Dejie
authors.initials: D
authors.lastname: Liang
authors.forename: Fengyang
authors.initials: F
authors.lastname: Li
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Song
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D057809:HEK293 Cells; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D055666:Lipopeptides / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051194:Toll-Like Receptor 1 / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D016207:Cytokines; D050838:Interferon Regulatory Factor-3; D055666:Lipopeptides; D008070:Lipopolysaccharides; C490500:Ly78 protein, mouse; C113553:Ly86 protein, mouse; D008562:Membrane Glycoproteins; D016328:NF-kappa B; C508075:Pam(3)CSK(4) peptide; D012333:RNA, Messenger; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051550:I-kappa B Kinase