title: Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
abstract: In this work highly localized femtosecond laser ablation is used to dissect single axons within a living Caenorhabditis elegans (C. elegans). We present a multimodal imaging methodology for the assessment of the collateral damage induced by the laser. This relies on the observation of the tissues surrounding the targeted region using a combination of different high resolution microscopy modalities. We present the use of Second Harmonic Generation (SHG) and Polarization Sensitive SHG (PSHG) to determine damage in the neighbor muscle cells. All the above is done using a single instrument: multimodal microscopy setup that allows simultaneous imaging in the linear and non-linear regimes and femtosecond-laser ablation.
authors.affiliation: ICFO-Institut de Ciencies Fotoniques, Mediterranean Technology Park, Castelldefels, Barcelona, Spain.
authors.forename: Susana I C O
authors.initials: SI
authors.lastname: Santos
authors.forename: Manoj
authors.initials: M
authors.lastname: Mathew
authors.forename: Omar E
authors.initials: OE
authors.lastname: Olarte
authors.forename: Sotiris
authors.initials: S
authors.lastname: Psilodimitrakopoulos
authors.forename: Pablo
authors.initials: P
authors.lastname: Loza-Alvarez
mesh_terms: D000818:Animals; D019771:Axotomy / Q000009:adverse effects / Q000379:methods*; D017173:Caenorhabditis elegans; D053685:Laser Therapy / Q000009:adverse effects / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D008859:Microscopy, Polarization / Q000379:methods*