title: Combined B- and T-cell deficiency does not protect against obesity-induced glucose intolerance and inflammation.
abstract: Obesity-induced inflammation is associated with insulin resistance and morphologically characterized by macrophage influx into the adipose tissue. Recently, various other immune cells, including B- and T-cells, have been shown to participate in modulating adipose tissue inflammation during the development of obesity. We show that HFD-feeding modulates the influx of B and T-cells into adipose tissue of obese animals, suggestive of a role of the adaptive immune system in the development of adipose tissue inflammation. Despite a lower bodyweight after HFD-feeding, gene expression levels of CD68, F4/80 and MCP-1 in white adipose tissue were enhanced in SCID animals that lack B- and T-cells. Moreover, conditioned medium from adipose tissue explants of HFD-fed SCID mice revealed increased release of IL-6 and CXCL1 compared to WT animals. Compared to WT mice, glucose tolerance was impaired in B- and T-cell deficient mice after HFD-feeding. Thus, complete B- and T-cell deficiency does not protect against HFD-induced adipose tissue inflammation and glucose intolerance. In contrast, SCID mice showed an increased pro-inflammatory status at the level of the adipose tissue in some cytokines. Our findings suggest that a delicate balance between various B- and T-cell populations controls adipose tissue inflammation.
authors.affiliation: Department of General Internal Medicine, Radboud University Medical Centre, Nijmegen Institute of Inflammation Immunity and Infection (N4i), Nijmegen, The Netherlands. d.ballak@aig.umcn.nl
authors.forename: Dov B
authors.initials: DB
authors.lastname: Ballak
authors.forename: Rinke
authors.initials: R
authors.lastname: Stienstra
authors.forename: Anneke
authors.initials: A
authors.lastname: Hijmans
authors.forename: Leo A B
authors.initials: LA
authors.lastname: Joosten
authors.forename: Mihai G
authors.initials: MG
authors.lastname: Netea
authors.forename: Cees J
authors.initials: CJ
authors.lastname: Tack
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001835:Body Weight; D059305:Diet, High-Fat; D004822:Epididymis / Q000473:pathology; D005247:Feeding Behavior; D018149:Glucose Intolerance / Q000150:complications* / Q000473:pathology; D007249:Inflammation / Q000150:complications* / Q000473:pathology; D008212:Lymphocyte Depletion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D009765:Obesity / Q000150:complications* / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000378:metabolism*