title: Evaluation of in vitro antioxidant effect of new mono and diselenides.
abstract: This study was designed to examine the antioxidant activity in vitro of novel mono- and diselenide compounds. We compared whether the formation of p-methyl-selenol from compounds 1-phenyl-3-(p-tolylselanyl)propan-2-amine (C1) and 1,2-dip-tolyldiselenide (C4) and o-methoxy-selenol from compounds 1-(2-methoxyphenylselanyl)-3-phenylpropan-2-amine (C2) and 1,2-bis(2-methoxyphenyl)diselenide (C3) may be involved in their antioxidant effects. The compounds were tested against Fe(II) and sodium nitroprusside (SNP)-induced lipid peroxidation in rat brain and liver homogenates. Likewise, the antioxidant capacity of the compounds was assessed by their ability to decolorize the DPPH radical as well as the Fe(II) chelating assay through the reduction of molybdenum(VI) (Mo6+) to molybdenum(V) (Mo5+). This colorimetric assay was also used to quantify thiol peroxidase (GPx) and oxidase activity and thioredoxin reductase (TrxR) activity. The results showed that the novel selenide compounds inhibit the thiobarbituric acid reactive species (TBARS) induced by different pro-oxidants, but the monoselenides effects were significant only at concentrations higher than the concentrations of the diselenides. Similarly, the total antioxidant activity was higher in the diselenides. Moreover, GPx and TrxR activity was only observed for the diselenides, which indicates that these compounds are more stable selenol molecules than monoselenides.
authors.affiliation: Departmento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, Brazil.
authors.forename: Sílvio Terra
authors.initials: ST
authors.lastname: Stefanello
authors.forename: Alessandro S
authors.initials: AS
authors.lastname: Prestes
authors.forename: Tade
authors.initials: T
authors.lastname: Ogunmoyole
authors.forename: Syed M
authors.initials: SM
authors.lastname: Salman
authors.forename: Ricardo S
authors.initials: RS
authors.lastname: Schwab
authors.forename: Caroline R
authors.initials: CR
authors.lastname: Brender
authors.forename: Luciano
authors.initials: L
authors.lastname: Dornelles
authors.forename: João B T
authors.initials: JB
authors.lastname: Rocha
authors.forename: Félix A A
authors.initials: FA
authors.lastname: Soares
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009249:NADP / Q000378:metabolism; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D054481:Thioredoxin Reductase 1 / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016566:Organoselenium Compounds; D017392:Thiobarbituric Acid Reactive Substances; D009249:NADP; D005979:Glutathione Peroxidase; D054481:Thioredoxin Reductase 1; C516126:Txnrd1 protein, rat