title: The Trypanosoma brucei telomerase RNA (TER) homologue binds core proteins of the C/D snoRNA family.
abstract: Trypanosome protozoan parasites are the causative agents of devastating diseases. Trypanosome telomeres grow in an uncontrolled manner and the variant surface glycoprotein (VSG) genes are located in subtelomeric domains. The gene encoding telomerase reverse transcriptase (TERT) was identified and in this study, we describe the Trypanosoma brucei telomerase RNA (TER). TER RNA is bound by the core proteins of the C/D small nucleolar RNA (snoRNA) family and associates with the methyltransferase-associated protein (MTAP), whose homologue binds to mammalian TER. Silencing of TbTER resulted in telomere shortening. This is the first report of a TER that binds the C/D snoRNA core proteins.
authors.affiliation: The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: Sachin Kumar
authors.initials: SK
authors.lastname: Gupta
authors.forename: Liat
authors.initials: L
authors.lastname: Kolet
authors.forename: Tirza
authors.initials: T
authors.lastname: Doniger
authors.forename: Viplov Kumar
authors.initials: VK
authors.lastname: Biswas
authors.forename: Ron
authors.initials: R
authors.lastname: Unger
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Tzfati
authors.forename: Shulamit
authors.initials: S
authors.lastname: Michaeli
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D011485:Protein Binding; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000235:genetics* / Q000378:metabolism*; D020718:Ribonucleoproteins, Small Nucleolar / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics
chemical_list: D020537:RNA, Small Nucleolar; D020718:Ribonucleoproteins, Small Nucleolar; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase