title: Anti-inflammatory lanostanoids and lactone derivatives from Antrodia camphorata.
abstract: Four new lanostanoids, ethyl lucidenate A (1), ethyl lucidenate F (2), 15-O-acetylganolucidate A (3), and 3,11,15,23-tetraoxo-27ξ-lanosta-8,16-dien-26-oic acid (4), and two new lactone derivatives, 5-hydroxy-5-(methoxymethyl)-4-methylfuran-2(5H)-one (5) and 3-(4-methoxy-2-oxo-2H-pyran-6-yl)propanoic acid (6), together with four known compounds, 11α-hydroxy-3,7-dioxolanost-8,24(E)-dien-26- oic acid (7), 3,7,11-trioxo-5α-lanosta-8,24(E)-dien-26-oic acid (8), methyl 3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate (9), and ethyl 3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate (10), were characterized from Antrodia camphorata. The structures of these new compounds were determined by analysis of their spectroscopic data, including 1D and 2D NMR experiments. Ten components were evaluated for anti-inflammatory activity by examining their effect on LPS-iNOS-dependent NO production in murine macrophage (RAW 264.7) cells. Among them, compounds 1, 3, 7, 8, 9, and 10 significantly suppressed the NO concentration in LPS-treated RAW 264.7 cells with IC50 values ≤ 10 μM.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Chih-Chuang
authors.initials: CC
authors.lastname: Liaw
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Guan-Jhong
authors.initials: GJ
authors.lastname: Huang
authors.forename: Yao-Ching
authors.initials: YC
authors.lastname: Tsai
authors.forename: Shih-Chang
authors.initials: SC
authors.lastname: Chien
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Wu
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Wang
authors.forename: Louis Kuoping
authors.initials: LK
authors.lastname: Chao
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
authors.forename: Hui-Chi
authors.initials: HC
authors.lastname: Huang
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D055491:Antrodia / Q000737:chemistry*; D007810:Lanosterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism; D013624:Taiwan
chemical_list: C582642:15-O-acetylganolucidate A; D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; C582641:ethyl lucidenate A; D007810:Lanosterol; D009569:Nitric Oxide