title: Scratch2 prevents cell cycle re-entry by repressing miR-25 in postmitotic primary neurons.
abstract: During the development of the nervous system the regulation of cell cycle, differentiation, and survival is tightly interlinked. Newly generated neurons must keep cell cycle components under strict control, as cell cycle re-entry leads to neuronal degeneration and death. However, despite their relevance, the mechanisms controlling this process remain largely unexplored. Here we show that Scratch2 is involved in the control of the cell cycle in neurons in the developing spinal cord of the zebrafish embryo. scratch2 knockdown induces postmitotic neurons to re-enter mitosis. Scratch2 prevents cell cycle re-entry by maintaining high levels of the cycle inhibitor p57 through the downregulation of miR-25. Thus, Scratch2 appears to safeguard the homeostasis of postmitotic primary neurons by preventing cell cycle re-entry.
authors.affiliation: Instituto de Neurociencias, CSIC-UMH, San Juan de Alicante 03550, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Rodríguez-Aznar
authors.forename: Alejandro
authors.initials: A
authors.lastname: Barrallo-Gimeno
authors.forename: M Angela
authors.initials: MA
authors.lastname: Nieto
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006706:Homeostasis / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D050761:Cyclin-Dependent Kinase Inhibitor p57; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D035683:MicroRNAs; D014157:Transcription Factors; D029961:Zebrafish Proteins; D000097573:Red Fluorescent Protein; C581230:MIRN25 microRNA, zebrafish; C561421:scrt2 protein, zebrafish