title: Characterization of five ECF sigma factors in the genome of Pseudomonas syringae pv. syringae B728a.
abstract: Pseudomonas syringae pv. syringae B728a, a bacterial pathogen of bean, utilizes large surface populations and extracellular signaling to initiate a fundamental change from an epiphytic to a pathogenic lifestyle. Extracytoplasmic function (ECF) sigma (σ) factors serve as important regulatory factors in responding to various environmental signals. Bioinformatic analysis of the B728a genome revealed 10 ECF sigma factors. This study analyzed deletion mutants of five previously uncharacterized ECF sigma factor genes in B728a, including three FecI-type ECF sigma factors (ECF5, ECF6, and ECF7) and two ECF sigma factors placed in groups ECF11 and ECF18. Transcriptional profiling by qRT-PCR analysis of ECF sigma factor mutants was used to measure expression of their associated anti-sigma and outer membrane receptor proteins, and expression of genes associated with production of extracellular polysaccharides, fimbriae, glycine betaine and syringomycin. Notably, the B728aΔecf7 mutant displayed reduced swarming and had decreased expression of CupC fimbrial genes. Growth and pathogenicity assays, using a susceptible bean host, revealed that none of the tested sigma factor genes are required for in planta growth and lesion formation.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Poulami Basu
authors.initials: PB
authors.lastname: Thakur
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: Vaughn-Diaz
authors.forename: Jessica W
authors.initials: JW
authors.lastname: Greenwald
authors.forename: Dennis C
authors.initials: DC
authors.lastname: Gross
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001692:Biological Transport / Q000187:drug effects / Q000235:genetics; D004777:Environment; D005110:Extracellular Space / Q000187:drug effects / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D016680:Genome, Bacterial / Q000187:drug effects / Q000235:genetics*; D007501:Iron / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D010641:Phenotype; D011134:Polysaccharides / Q000378:metabolism; D044224:Pseudomonas syringae / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D018085:Regulon / Q000235:genetics; D017384:Sequence Deletion; D012808:Sigma Factor / Q000172:deficiency / Q000235:genetics*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014050:Toluene / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides; D012808:Sigma Factor; C032677:syringomycin; D014050:Toluene; D007501:Iron