title: Enhancer trap infidelity in Drosophila optomotor-blind.
abstract: Reporter gene activity in enhancer trap lines is often implicitly assumed to mirror quite faithfully the endogenous expression of the "trapped" gene, even though there are numerous examples of enhancer trap infidelity. optomotor-blind (omb) is a 160 kb gene in which 16 independent P-element enhancer trap insertions of three different types have been mapped in a range of more than 60 kb. We have determined the expression pattern of these elements in wing, eye-antennal and leg imaginal discs as well as in the pupal tergites. We noted that one pGawB insertion (omb (P4) ) selectively failed to report parts of the omb pattern even though the missing pattern elements were apparent in all other 15 lines. We ruled out that omb (P4) was defective in the Gal4 promoter region or had inactivated genomic enhancers in the integration process. We propose that the Gal4 reporter gene in pGawB may be sensitive to orientation or promoter proximity effects.
authors.affiliation: Institute of Genetics, Johannes Gutenberg-Universität, Mainz, Germany.
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Mayer
authors.forename: Sören
authors.initials: S
authors.lastname: Diegelmann
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Abassi
authors.forename: Fred
authors.initials: F
authors.lastname: Eichinger
authors.forename: Gert O
authors.initials: GO
authors.lastname: Pflugfelder
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D002874:Chromosome Mapping; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005121:Extremities; D005123:Eye / Q000378:metabolism; D017930:Genes, Reporter; D060227:Imaginal Discs / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011679:Pupa / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism; D014921:Wings, Animal / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D020825:T-Box Domain Proteins; C072962:bi protein, Drosophila
keywords: Drosophila; abdominal tergites; enhancer trap; imaginal disc; optomotor-blind