title: Purple sea urchin Strongylocentrotus purpuratus gamete manipulation using optical trapping and microfluidics.
abstract: A system has been developed that allows for optical and fluidic manipulation of gametes. The optical manipulation is performed by using a single-point gradient trap with a 40× oil immersion PH3 1.3 NA objective on a Zeiss inverted microscope. The fluidic manipulation is performed by using a custom microfluidic chamber designed to fit into the short working distance between the condenser and objective. The system is validated using purple sea urchin Strongylocentrotus purpuratus gametes and has the potential to be used for mammalian in vitro fertilization and animal husbandry.
authors.affiliation: University of California, Department of Bioengineering, San Diego, 9500 Gilman Dr., La Jolla, California 92093, USA. cchandsa@ucsd.edu
authors.forename: Charlie
authors.initials: C
authors.lastname: Chandsawangbhuwana
authors.forename: Linda Z
authors.initials: LZ
authors.lastname: Shi
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Zhu
authors.forename: Michael W
authors.initials: MW
authors.lastname: Berns
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D005854:Germ Cells / Q000166:cytology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers; D047270:Strongylocentrotus purpuratus / Q000166:cytology*