title: Rapid diagnosis of goose viral infections by multiplex PCR.
abstract: Goose parvovirus (GPV), newcastle disease virus (NDV), goose herpesvirus (GHV) and goose adenovirus (GAV) are considered collectively to be four of the most important and widespread viruses of geese. Because all of these viruses cause similar pathological changes, histological differentiation among these viruses is difficult. A reliable, specific and sensitive multiplex PCR (mPCR) assay was developed for the combined detection of GPV, NDV, GHV and GAV in clinical samples of geese. Using the mPCR technique, single infections with GPV (28/76; 36.8%), NDV (9/76; 11.8%), GHV (3/76; 3.9%) and GAV (12/76; 15.8%) were identified in the samples; co-infections with GAV and either GPV or NDV (31.6%; 24/76) were also identified with this approach. The results for all of the samples tested were the same in both the uPCR and mPCR systems. The mPCR approach is considered to be useful for routine molecular diagnosis and epidemiological applications in geese.
authors.affiliation: National Engineering Research Center for Poultry, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai 200241, China.
authors.forename: Zongyan
authors.initials: Z
authors.lastname: Chen
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Li
authors.forename: Guoxin
authors.initials: G
authors.lastname: Li
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Jiang
authors.forename: Liping
authors.initials: L
authors.lastname: Yan
authors.forename: Chunchun
authors.initials: C
authors.lastname: Meng
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Tong
authors.forename: Guangqing
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D060085:Coinfection / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D005777:Geese; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods*; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology