title: The protective effect of silybin against lasalocid cytotoxic exposure on chicken and rat cell lines.
abstract: Lasalocid, an ionophore coccidiostat, extensive use implies a risk of toxicological impacts. Protective effects of silybin, a herbal compound of Silybum marianum, are reported elsewhere. The aim of this study was to compare effects of the combined use of lasalocid and silybin in chicken hepatoma cells (LMH) and rat myoblasts (L6) cell lines cultures. The cytoprotective effect resulting from an interaction of both pharmaceuticals was measured with the help of MTT reduction and, coomassie brilliant blue binding (CBB) and LDH release assays. Isobolography and the combination index (CI) estimated the nature and scale of interaction. In all performed tests, the lowest lasalocid EC50-values were obtained for chicken hepatocytes. In the rat myoblasts cultures, the lowest lasalocid EC50-values were found with LDH test. Simultaneously, a lack of silybin cytotoxic effect was proven for the studied cell lines. An interaction between both substances led to a considerable decrease of lasalocid cytotoxicity. The isobolograms and combination index showed a significant antagonistic nature of silybin effect in the course of lasalocid cytotoxicity. It is concluded that the mechanism of cytoprotection results from complex reaction at biochemical and biophysical endpoints during chicken hepatocytes and rat myoblasts cell lines exposure to silybin and lasalocid co-action.
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Radko
authors.forename: Wojciech
authors.initials: W
authors.lastname: Cybulski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rzeski
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002645:Chickens; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D007476:Ionophores / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007832:Lasalocid / Q000633:toxicity*; D032446:Myoblasts / Q000378:metabolism; D051381:Rats; D012394:Rosaniline Dyes; D000077385:Silybin; D012838:Silymarin / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000975:Antioxidants; D007476:Ionophores; D012394:Rosaniline Dyes; D012838:Silymarin; D013778:Tetrazolium Salts; D013844:Thiazoles; D000077385:Silybin; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; C004692:coomassie Brilliant Blue; D007832:Lasalocid