title: Purification antibody by thiophilic magnetic sorbent modified with 2-mercapto-1-methylimidazol.
abstract: An efficient strategy based on the thiophilic magnetic beads was developed to isolate immunoglobulin G (IgG) from human plasma with high efficiency. Depending on the improved surface modification, the surface density of thiophilic ligand (2-mercapto-1-methylimidazol) reached 1.67mmolg(-1) resulting in a high adsorption capacity (qmax=20.7mgg(-1)) toward IgG. This purification of IgG could be carried out in some physiological conditions due to the heterocyclic structure of 2-mercapto-1-methylimidazol. It is found that the recognition region between the thiophilic ligand and IgG was mainly focused on the Fab fragments of IgG using the technology of enzyme digestion. Furthermore, the bound antibodies could be easily recovered without loss of their bioactivity at low salt concentration. These magnetic carries brought great potentials in large-scale antibody purification from human plasma attributing to their high specificity and efficiency, stable structure, sensitive magnetic response and excellent reusability.
authors.affiliation: College of Material Science and Engineering, Huaqiao University, XiaMen 362021, Fujian, China.
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Lin
authors.forename: YingXue
authors.initials: Y
authors.lastname: Zhang
authors.forename: ChunJiao
authors.initials: C
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Qian
mesh_terms: D000327:Adsorption; D001666:Binding Sites, Antibody; D017743:Equipment Reuse; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D007700:Kinetics; D059346:Magnets; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: C107674:2-mercapto-1-methylimidazole; D007093:Imidazoles; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G