title: Reversal of elution order of N-(2,4-dinitrophenyl)-proline and N-(2,4-dinitrophenyl)-serine in HPLC by BSA chiral stationary phase.
abstract: N-(2,4-dinitrophenyl)-proline and N-(2,4-dinitrophenyl)-serine were enantiomerically resolved on the BSA chiral stationary phase by HPLC in reversed-phase mode. Effects of chromatographic conditions on enantioseparation and elution order have been investigated in detail. For these two samples, reversal of enantiomer elution order was observed by changing buffer pH, the content of acetonitrile, or alcohol modifiers in mobile phase, which is firstly reported in the BSA chiral stationary phase studies. More interestingly, combined effect between buffer pH and the content of acetonitrile was also observed. In addition, coelution range of enantiomers varied along with the content of acetonitrile in mobile phase.
authors.affiliation: Institute of Special Materials, School of Chemistry and Environment, South China Normal University, Guangzhou, P R China.
authors.forename: Qiuyun
authors.initials: Q
authors.lastname: Wang
authors.forename: Yajin
authors.initials: Y
authors.lastname: Xiong
authors.forename: Baozhu
authors.initials: B
authors.lastname: Lu
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Shengrun
authors.initials: S
authors.lastname: Zheng
authors.forename: Weiguang
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011392:Proline / Q000031:analogs & derivatives* / Q000302:isolation & purification; D012694:Serine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D012710:Serum Albumin, Bovine; D012694:Serine; D011392:Proline