title: Telomerase activity in human brain tumors: astrocytoma and meningioma.
abstract: Somatic cells do not have telomerase activity but immortalized cell lines and more than 85 % of the cancer cells show telomerase activation to prevent the telomere from progressive shortening. The activation of this enzyme has been found in a variety of human tumors and tumor-derived cell lines, but only few studies on telomerase activity in human brain tumors have been reported. Here, we evaluated telomerase activity in different grades of human astrocytoma and meningioma brain tumors. In this study, assay for telomerase activity performed on 50 eligible cases consisted of 26 meningioma, 24 astrocytoma according to the standard protocols. In the brain tissues, telomerase activity was positive in 39 (65 %) of 50 patients. One sample t test showed that the telomerase activity in meningioma and astrocytoma tumors was significantly positive entirely (P < 0.001). Also, grade I of meningioma and low grades of astrocytoma (grades I and II) significantly showed telomerase activity. According to our results, we suggest that activation of telomerase is an event that starts mostly at low grades of brain including meningioma and astrocytoma tumors.
authors.affiliation: Pediatric Inherited Diseases Research Center, Genetics and Molecular Biology Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. mkheirollahi@med.mui.ac.ir
authors.forename: Majid
authors.initials: M
authors.lastname: Kheirollahi
authors.forename: Masoud
authors.initials: M
authors.lastname: Mehrazin
authors.forename: Naser
authors.initials: N
authors.lastname: Kamalian
authors.forename: Javad
authors.initials: J
authors.lastname: Mohammadi-asl
authors.forename: Parvin
authors.initials: P
authors.lastname: Mehdipour
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000201:enzymology* / Q000473:pathology; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008579:Meningioma / Q000201:enzymology* / Q000473:pathology; D008875:Middle Aged; D060787:Neoplasm Grading; D000071681:Tartrate-Resistant Acid Phosphatase; D019098:Telomerase / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D019098:Telomerase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase