title: Luminescence-based in vivo monitoring of NF-κB activity through a gene delivery approach.
abstract: BACKGROUND
  Monitoring activity of specific signaling pathways in vivo is challenging and requires highly sensitive methods to detect dynamic perturbations in whole organisms.
  
  
  RESULTS
  In vivo gene delivery of a luciferase reporter followed by bioluminiscence imaging allows measuring NF-κB activity in mice liver and lungs.
  
  
  CONCLUSIONS
  This protocol allows a direct measure of NF-κB activity through quantification of bioluminescence signal, demonstrating its accuracy and sensitivity in different animal models and experimental conditions. Variants could be also applied for the analysis of NF-κB activity in different tissues or for studying other signaling pathways in vivo.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, Oviedo, 33006, Spain. jmpf@uniovi.es.
authors.forename: Fernando G
authors.initials: FG
authors.lastname: Osorio
authors.forename: Jorge
authors.initials: J
authors.lastname: de la Rosa
authors.forename: José Mp
authors.initials: JM
authors.lastname: Freije