title: Identification of differential expression genes in leaves of rice (Oryza sativa L.) in response to heat stress by cDNA-AFLP analysis.
abstract: High temperature impedes the growth and productivity of various crop species. To date, rice (Oryza sativa L.) has not been exploited to understand the molecular basis of its abnormally high level of temperature tolerance. To identify transcripts induced by heat stress, twenty-day-old rice seedlings of different rice cultivars suffering from heat stress were treated at different times, and differential gene expression analyses in leaves were performed by cDNA-AFLP and further verified by real-time RT-PCR. In aggregate, more than three thousand different fragments were indentified, and 49 fragments were selected for the sequence and differential expressed genes were classified functionally into different groups. 6 of 49 fragments were measured by real-time RT-PCR. In addition, the variations of three different polyamine contents in response to heat stress through high-performance liquid chromatography (HPLC) analysis were also performed. The results and their direct and indirect relationships to heat stress tolerance mechanism were discussed.
authors.affiliation: College of Life Science, Nantong University, Nantong, Jiangsu 226019, China.
authors.forename: Yunying
authors.initials: Y
authors.lastname: Cao
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.forename: Youzhong
authors.initials: Y
authors.lastname: Lang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Yu
authors.forename: Jianchang
authors.initials: J
authors.lastname: Yang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013045:Species Specificity; D013312:Stress, Physiological
chemical_list: D012333:RNA, Messenger