title: Enhancing depth of focus in tilted microfluidics channels by digital holography.
abstract: In this Letter we propose a method to enhance the limited depth of field (DOF) in optical imaging systems, through digital holography. The proposed approach is based on the introduction of a cubic phase plate into the diffraction integral, analogous to what occurs in white-light imaging systems. By this approach we show that it is possible to improve the DOF and to recover the extended focus image of a tilted object in a single reconstruction step. Moreover, we demonstrate the possibility of obtaining well-focused biological cells flowing into a tilted microfluidic channel.
authors.affiliation: CNR-Istituto Nazionale di Ottica Applicata, Pozzuoli, Italy. marcella.matrecano@ino.it
authors.forename: Marcella
authors.initials: M
authors.lastname: Matrecano
authors.forename: Melania
authors.initials: M
authors.lastname: Paturzo
authors.forename: Andrea
authors.initials: A
authors.lastname: Finizio
authors.forename: Pietro
authors.initials: P
authors.lastname: Ferraro
mesh_terms: D000818:Animals; D002417:Cattle; D006696:Holography / Q000379:methods*; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology