title: Enzymatic production of ferulic acid from defatted rice bran by using a combination of bacterial enzymes.
abstract: Ferulic acid (FA), which is present in the cell walls of some plants, is best known for its antioxidant property. By combining a commercial enzyme that shows FA esterase activity with several Streptomyces carbohydrate-hydrolyzing enzymes, we succeeded in enhancing the enzymatic production of FA from defatted rice bran. In particular, the combination of three xylanases, an α-L-arabinofuranosidase, and an acetyl xylan esterase from Streptomyces spp. produced the highest increase in the amount of released FAs among all the enzymes in the Streptomyces enzymes library. This enzyme combination also had an effect on FA production from other biomasses, such as raw rice bran, wheat bran, and corncob.
authors.affiliation: Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Research Institute for Biological Sciences (RIBS), 7549-1 Kibichuo-cho, Kaga-gun, Okayama, 716-1241, Japan.
authors.forename: Misugi
authors.initials: M
authors.lastname: Uraji
authors.forename: Masayo
authors.initials: M
authors.lastname: Kimura
authors.forename: Yosikazu
authors.initials: Y
authors.lastname: Inoue
authors.forename: Kayoko
authors.initials: K
authors.lastname: Kawakami
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kumagai
authors.forename: Koichi
authors.initials: K
authors.lastname: Harazono
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hatanaka
mesh_terms: D000115:Acetylesterase / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D001709:Biotechnology; D003373:Coumaric Acids / Q000737:chemistry; D004043:Dietary Fiber / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D012275:Oryza / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*; D014990:Xylans / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003373:Coumaric Acids; D004043:Dietary Fiber; D014990:Xylans; C004999:ferulic acid; D000115:Acetylesterase; C081851:acetylxylan esterase; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase