title: In vitro study of the effect of dog food on the adsorptive capacity of activated charcoal.
abstract: OBJECTIVE
  To evaluate the effect of dog food on the adsorptive capacity of activated charcoal.
  
  
  METHODS
  In vitro laboratory study.
  
  
  METHODS
  University veterinary teaching hospital.
  
  
  METHODS
  None.
  
  
  METHODS
  None.
  
  
  METHODS
  A fixed quantity of acetaminophen (50 mg) was added to a fixed quantity of activated charcoal (1 g), mixed with varying amounts of dog food (2-14 g). The admixture was agitated for 5 minutes, incubated at 38.5°C for 1 hour and then centrifuged for 30 minutes. The concentration of residual, nonadsorbed acetaminophen in the supernatant was quantitatively assayed by reverse phase high-pressure liquid chromatography with ultraviolet detection. Data were tested by linear regression analysis and statistical significance was set at P < 0.05.
  
  
  RESULTS
  A statistically significant reduction in the adsorptive capacity of activated charcoal was demonstrated with increasing amounts of dog food (R(2) = 0.54; P = 0.0018). However, all measurements of residual acetaminophen were less than 100 mg/L, representing a reduction in acetaminophen concentration of more than 98.6%.
  
  
  CONCLUSIONS
  The addition of dog food to activated charcoal reduces its total adsorptive capacity for acetaminophen. However, this reduction in adsorptive capacity is unlikely to be clinically significant in the presence of both the formulation of dog food and the ratio of dog food to charcoal used in this study.
authors.affiliation: Department of Clinical Sciences and Services, The Royal Veterinary College, North Mymms, AL97TA, United Kingdom. vethew@gmail.com
authors.forename: Helen E
authors.initials: HE
authors.lastname: Wilson
authors.forename: Karen R
authors.initials: KR
authors.lastname: Humm
mesh_terms: D000082:Acetaminophen / Q000737:chemistry*; D000327:Adsorption; D018712:Analgesics, Non-Narcotic / Q000737:chemistry*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002606:Charcoal / Q000737:chemistry*; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results
chemical_list: D018712:Analgesics, Non-Narcotic; D002606:Charcoal; D000082:Acetaminophen