title: High fat diet partially attenuates fermentation responses in rats fed resistant starch from high-amylose maize.
abstract: OBJECTIVE
  The effects of type 2 resistant starch from high-amylose maize (HAM-RS2) in rodents fed with low-fat diets were demonstrated in previous studies. Fish oil is also reported to reduce body fat. In the current study, the effects of high fat and fish oil on HAM-RS2 feeding in rats were investigated.
  
  
  METHODS
  Rats were fed 0 or 27% (weight) HAM-RS2 with low (15% energy) or high fat (42% energy) diets that included 0 or 10% (energy) tuna oil to test the effect of HAM-RS2 in diet-induced obesity and effects of tuna oil. Data were analyzed as 2 × 2 × 2 factorial.
  
  
  RESULTS
  Rats fed HAM-RS2 had decreased cecal contents pH, increased cecal and cecal contents weight, increased cecal contents acetate, propionate, and butyrate, increased GLP-1 and PYY, and decreased abdominal fat. However, high fat partially attenuated effects of HAM-RS2, but increased GLP-1 active. Dietary tuna oil had limited effects at concentration used.
  
  
  CONCLUSIONS
  Results demonstrated that a high fat diet partially attenuates the response to HAM-RS2. The mechanism may center on reduced levels of cecal contents propionate and butyrate and reduced serum PYY. This study demonstrated that with consumption of high fat, HAM-RS2 produces fermentation but results in partial attenuation of effects.
authors.affiliation: Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Charrier
authors.forename: Roy J
authors.initials: RJ
authors.lastname: Martin
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: McCutcheon
authors.forename: Anne M
authors.initials: AM
authors.lastname: Raggio
authors.forename: Felicia
authors.initials: F
authors.lastname: Goldsmith
authors.forename: M'famara
authors.initials: M
authors.lastname: Goita
authors.forename: Reshani N
authors.initials: RN
authors.lastname: Senevirathne
authors.forename: Ian L
authors.initials: IL
authors.lastname: Brown
authors.forename: Christine
authors.initials: C
authors.lastname: Pelkman
authors.forename: June
authors.initials: J
authors.lastname: Zhou
authors.forename: John
authors.initials: J
authors.lastname: Finley
authors.forename: Holiday A
authors.initials: HA
authors.lastname: Durham
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keenan
mesh_terms: D050153:Abdominal Fat / Q000033:anatomy & histology; D000688:Amylose / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000494:pharmacology*; D004435:Eating / Q000502:physiology; D002149:Energy Intake / Q000502:physiology; D005285:Fermentation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D013213:Starch; D000688:Amylose