title: MyD88 as a target of microRNA-203 in regulation of lipopolysaccharide or Bacille Calmette-Guerin induced inflammatory response of macrophage RAW264.7 cells.
abstract: MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of microRNA-203 (miR-203) in the immunological regulation, the function of miR-203 was explored in the macrophage RAW264.7 cells against lipopolysaccharide (LPS) or Bacille Calmette-Guerin (BCG) stimulation. The results evidenced that myeloid differentiation factor 88 (MyD88) was a novel target of miR-203, miR-203 was capable of directly targeting the 3' untranslated region (3'UTR) of MyD88 and post-transcriptionally down-regulating the expression of protein. In addition, an overexpression of miR-203 in RAW264.7 cells was correlated with repressions of MyD88, as well as its downstream signaling of NF-κB (NF-κB1), TNF-α and IL-6. These results suggest that miR-203 may be an important regulator in macrophages against LPS or mycobacteria infection, which may through a mechanism of negatively regulating MyD88-dependent Toll-like receptors signaling pathway.
authors.affiliation: General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
authors.forename: Xuelan
authors.initials: X
authors.lastname: Huang
authors.forename: Zhaobo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.forename: Guangxian
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009163:Mycobacterium bovis / Q000276:immunology*; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics* / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology
chemical_list: D018836:Inflammation Mediators; D008070:Lipopolysaccharides; C575934:MIRN203 microRNA, mouse; D035683:MicroRNAs; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88