title: Transforming growth factor beta 1 and monocyte chemoattractant protein-1 as prognostic markers of diabetic nephropathy.
abstract: We aimed to find the relationship between serum transforming growth factor beta 1(TGF-β(1)) and urinary monocyte chemoattractant protein-1 (MCP-1) throughout the course of diabetic nephropathy (DN) and to assess the relationship between both levels and other parameters of renal injury such as albumin/creatinine ratio and estimated glomerular filtration rate (eGFR). Serum TGF-β(1), urinary MCP-1, eGFR, and glycosylated hemoglobin (HbA1c) were measured in 60 patients with type II diabetes mellitus with different degrees of nephropathy (20 patients with normoalbuminuria, 20 patients with microalbuminuria, and 20 patients with macroalbuminuria) and compared with 20 matched healthy control subjects. Both the levels of serum TGF-β(1) and urinary MCP-1 were significantly higher in patients with micro- and macroalbuminuria (137.8 ± 69.5 and 329.25 ± 41.46 ng/dl, respectively, for TGF-β(1) and 167.41 ± 50.23 and 630.87 ± 318.10 ng/g creatinine, respectively, for MCP-1) compared with normoalbuminuric patients and healthy controls (33.25 ± 17.5 and 29.64 ± 10.57 ng/dl, respectively, for TGF-β(1) and 63.85 ± 21.15 and 61.50 ± 24.81 ng/g creatinine, respectively, for MCP-1; p < 0.001). There was a positive significant correlation between the levels of serum TGF-β(1) and those of urinary MCP-1 (r = 0.73, p < 0.001). Also, serum TGF-β(1) and urinary MCP-1 correlated positively with HbA1c (r = 0.49 and 0.55, respectively, p < 0.05 for both) and inversely with eGFR (r = -0.69 and -0.60, respectively, p < 0.001 for both). We can conclude that serum TGF-β(1) and urinary MCP-1 can be used as the markers for detection of progression of DN. Antagonizing TGF-β(1) and MCP-1 might be helpful in attenuating the progression of nephropathy in diabetic patients.
authors.affiliation: 1Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: O G
authors.initials: OG
authors.lastname: Shaker
authors.forename: N A H
authors.initials: NA
authors.lastname: Sadik
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000652:urine; D018932:Chemokine CCL2 / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000652:urine; D003928:Diabetic Nephropathies / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D053773:Transforming Growth Factor beta1 / Q000097:blood*
chemical_list: D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1
keywords: Diabetic nephropathy; MCP-1; TGF-β1