title: Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer.
abstract: OBJECTIVE
  Several multigene expression-based tests offering prognostic and predictive information in hormone-receptor positive early breast cancer were established during the last years. These tests provide prognostic information on distant recurrences and can serve as an aid in therapy decisions. We analyzed the recently validated reverse-transcription-quantitative-real-time PCR-based multigene-expression Endopredict (EP)-test on 34 hormone-receptor positive breast-cancer cases and compared the EP scores with the Oncotype DX Recurrence-scores (RS) obtained from the same cancer samples.
  
  
  METHODS
  Formalin-fixed, paraffin-embedded invasive breast-cancer tissues from 34 patients were analyzed by the EP-test. Representative tumor blocks were analyzed with Oncotype DX prior to this study. Tumor tissue was removed from unstained slides, total-RNA was isolated and EP-analysis was performed blinded to Oncotype DX results.
  
  
  RESULTS
  Extraction of sufficient amounts of RNA and generation of valid EP-scores were possible for all 34 samples. EP classified 11 patients as low-risk and 23 patients as high-risk. RS Score defined 15 patients as low-risk, 10 patients as intermediate-risk in and 9 patients as high-risk. Major-discrepancy occurred in 6 of 34 cases (18%): Low-risk RS was classified as high-risk by EP in 6 cases. Combining the RS intermediate-risk and high-risk groups to a common group, the concordance between both tests was 76%. Correlation between continuous EP and RS-scores was moderate (Pearson-coefficient: 0.65 (p<0.01).
  
  
  CONCLUSIONS
  We observed a significant but moderate concordance (76%) and moderate correlation (0.65) between RS and EP Score. Differences in results can be explained by different weighting of biological motives covered by the two tests. Further studies are needed to explore the clinical relevance of discrepant test results with respect of outcome.
authors.affiliation: Institute of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland. zsuzsanna.varga@usz.ch
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Varga
authors.forename: Peter
authors.initials: P
authors.lastname: Sinn
authors.forename: Florian
authors.initials: F
authors.lastname: Fritzsche
authors.forename: Arthur
authors.initials: A
authors.lastname: von Hochstetter
authors.forename: Aurelia
authors.initials: A
authors.lastname: Noske
authors.forename: Peter
authors.initials: P
authors.lastname: Schraml
authors.forename: Christoph
authors.initials: C
authors.lastname: Tausch
authors.forename: Andreas
authors.initials: A
authors.lastname: Trojan
authors.forename: Holger
authors.initials: H
authors.lastname: Moch
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism; D012008:Recurrence; D055815:Young Adult
chemical_list: D019394:Ki-67 Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone