title: Production, purfication, molecular characterization and bioactivities of exopolysaccharides produced by the wine cap culinary-medicinal mushroom, Stropharia rugosoannulata 2# (higher Basidiomycetes).
abstract: The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Stropharia rugosoannulata 2# were determined using the orthogonal matrix method. The optimal defined medium (per liter) was 60.0 g sucrose, 6.0 g tryptone, 5 mM KH₂PO₄, and initial pH 7.0 at 28°C. In the optimal culture medium, the maximum EPS production was 9.967 g/L in shake-flask culture. One fraction of EPS was purified from the culture filtrates by size exclusion chromatography (SEC), and the molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The weight-average molar masses and the polydispersity ratio of the EPS fraction were determined to be 5.305 × 103 g/mol and 2.014, respectively. FTIR spectroscopy was used for obtaining vibrational spectra of the purified EPS fraction. The obvious characteristic absorption at 884.3 cm-1 revealed the existence of β configuration. Furthermore, the experiments in vitro indicated that S. rugosoannulata 2# EPS exhibit high antitumor and antioxidative effects.
authors.affiliation: College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R. China.
authors.forename: Peixin
authors.initials: P
authors.lastname: He
authors.forename: Lujing
authors.initials: L
authors.lastname: Geng
authors.forename: Jizhong
authors.initials: J
authors.lastname: Wang
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry / Q000378:metabolism*; D001713:Biphenyl Compounds; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008113:Liver Neoplasms / Q000188:drug therapy; D010851:Picrates; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl