title: Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines.
abstract: Here, we investigate the appropriate fluorescent proteins for use in the culture of synovial MH7A and myoblast C2C12 cells. Fluorescent signal intensities of 3 different fluorescent proteins were examined in these cell lines. The fluorescent intensity of transiently expressed AcGFP, DsRed, and mStrawberry were examined in these cell lines, and the influence of the amount of plasmid used on transfection efficiency and cell viability were investigated.
authors.affiliation: Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe, 650-8530 Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibasaki
authors.forename: Aika
authors.initials: A
authors.lastname: Fujita
authors.forename: Chihiro
authors.initials: C
authors.lastname: Usui
authors.forename: Sachiko
authors.initials: S
authors.lastname: Watanabe
authors.forename: Sachie
authors.initials: S
authors.lastname: Kitano
authors.forename: Hajime
authors.initials: H
authors.lastname: Sano
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Iwasaki
keywords: C2C12 cell; Cell viability; Fluorescent protein; MH7A cell; Transfection