title: Serum cross-reaction among virulence-associated trimeric autotransporters (VtaA) of Haemophilus parasuis.
abstract: Glässer's disease is a fibrinous polyserositis and polyarthritis of swine caused by the bacterium Haemophilus parasuis. Control by vaccination has been limited for years due to lack of cross-protection among strains. However, 6 trimeric autotransporters (VtaA) of the Nagasaki strain were shown to be antigenic and gave partial protection to a lethal challenge. The antigenic relationship among the VtaAs was examined by immunizing mice with individual VtaA showing that they cross-reacted by ELISA mainly with VtaA from the same group. When sera from protected and non-protected vaccinated piglets were examined no differences in VtaA cross-reactivity profiles were found. In addition, sera from commercial pigs immunized with a single VtaA (VtaA9) showed a wider range of VtaA cross-reaction, probably due to the previous colonization by H. parasuis. These results can help the development of new vaccine formulations against H. parasuis by allowing a rational VtaA selection.
authors.affiliation: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Barcelona, Spain.
authors.forename: Alex
authors.initials: A
authors.lastname: Olvera
authors.forename: Verónica
authors.initials: V
authors.lastname: Martínez-Moliner
authors.forename: Sonia
authors.initials: S
authors.lastname: Pina-Pedrero
authors.forename: Marta
authors.initials: M
authors.lastname: Pérez-Simó
authors.forename: Nuria
authors.initials: N
authors.lastname: Galofré-Milà
authors.forename: Mar
authors.initials: M
authors.lastname: Costa-Hurtado
authors.forename: Virginia
authors.initials: V
authors.lastname: Aragon
authors.forename: Albert
authors.initials: A
authors.lastname: Bensaid
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology*; D005260:Female; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000276:immunology* / Q000472:pathogenicity*; D007114:Immunization; D051379:Mice; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology*; D014611:Vaccination / Q000662:veterinary; D014774:Virulence / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial