title: Detection of human bocavirus in children with upper respiratory tract infection by polymerase chain reaction.
abstract: BACKGROUND
  The aim of this study was to investigate whether in children with middle ear effusions (MEE), adenoid and tonsil tissues are associated with human bocavirus (HBoV).
  
  
  METHODS
  A total of 124 patients (56 females (45.2%) and 68 males (54.8%)) with chronic adenotonsillitis and serous otitis media under the age of 15 were recruited. Two hundered four samples (113 adenoid (55.4%), 68 tonsil (33.3%), and 23 middle ear effusion (11.3%)) were analyzed for the presence of HBoV using polymerase chain reaction (PCR).
  
  
  RESULTS
  HBoV was detected in only 6 (4.8%) adenoid tissue samples each belonging to a different patient.
  
  
  CONCLUSIONS
  Our findings are consistent with the results of other studies, reporting approximately 5 - 10% of the samples being positive for HBoV. To understand the detailed role of HBoV in the etiology of RTI in children, further studies would be needed.
authors.affiliation: Antalya Education and Research Hospital, Department of ENT Head and Neck Surgery, Antalya, Turkey. hulinar@yahoo.com
authors.forename: Hulya
authors.initials: H
authors.lastname: Eyigor
authors.forename: Ustun
authors.initials: U
authors.lastname: Osma
authors.forename: Mete
authors.initials: M
authors.lastname: Eyigor
authors.forename: Mustafa Deniz
authors.initials: MD
authors.lastname: Yilmaz
authors.forename: Berna
authors.initials: B
authors.lastname: Gultekin
authors.forename: Murat
authors.initials: M
authors.lastname: Telli
authors.forename: Atakan
authors.initials: A
authors.lastname: Ozturan
authors.forename: Meral
authors.initials: M
authors.lastname: Gultekin
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D052660:Bocavirus / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010034:Otitis Media with Effusion / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000821:virology*; D014069:Tonsillitis / Q000821:virology
chemical_list: D017931:DNA Primers