title: The use of dansyl-calmodulin to study interactions with channels and other proteins.
abstract: Steady-state fluorescence spectroscopy is a biophysical technique widely employed to characterize -interactions between proteins in vitro. Only a few proteins naturally fluoresce in cells, but by covalently attaching fluorophores virtually all proteins can be monitored. One of the first extrinsic fluorescent probes to be developed, and that is still in use, is dansyl chloride. We have used this method to monitor the interaction of a variety of proteins, including ion channels, with the Ca(2+)-dependent regulatory protein calmodulin. Here we describe the preparation and use of dansyl-calmodulin (D-CaM).
authors.affiliation: Unidad de Biofísica (CSIC-UPV/EHU), Universidad del País Vasco, Leioa, Spain.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Alaimo
authors.forename: Covadonga
authors.initials: C
authors.lastname: Malo
authors.forename: Pilar
authors.initials: P
authors.lastname: Areso
authors.forename: Kerman
authors.initials: K
authors.lastname: Aloria
authors.forename: Oscar
authors.initials: O
authors.lastname: Millet
authors.forename: Alvaro
authors.initials: A
authors.lastname: Villarroel
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051658:KCNQ2 Potassium Channel / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: C035684:5-dimethylaminonaphthalene-1-sulfonyl-calmodulin; D002147:Calmodulin; D051658:KCNQ2 Potassium Channel