title: Exon array analysis of alternative splicing of genes in SOD1G93A transgenic mice.
abstract: Alternative splicing is a common strategy for creating functional diversities of proteins. While conventional identification of splice variants generally targets individual genes in amyotrophic lateral sclerosis, we present a novel exon-centric array that allows genome-wide identification of splice variants and concurrently provides analysis of gene expression. Compare 1 was asymptomatic SOD1G93A transgenic mice with nontransgenic littermates; compare 2 was symptomatic with asymptomatic transgenic mice. RT-PCR was performed to validate. Pathway and GO analysis were performed on abnormal genes. These findings could guide us to demonstrated the potential influence of mutant human CuZn-SOD1 and of splicing regulation in pathological processes.
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.forename: Yansu
authors.initials: Y
authors.lastname: Guo
authors.forename: Huifang
authors.initials: H
authors.lastname: Chen
authors.forename: Weisong
authors.initials: W
authors.lastname: Duan
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
mesh_terms: D017398:Alternative Splicing; D000690:Amyotrophic Lateral Sclerosis / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020033:Protein Isoforms; D013116:Spinal Cord / Q000201:enzymology / Q000473:pathology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D020033:Protein Isoforms; C109415:SOD1 G93A protein; D013482:Superoxide Dismutase