title: Evaluation of the protective effect of Beta glucan on amikacin ototoxicity using distortion product otoacoustic emission measurements in rats.
abstract: OBJECTIVE
  This experimental study investigated the possible protective effect of beta glucans on amikacin ototoxicity.
  
  
  METHODS
  Thirty-eight rats with normal distortion product otoacoustic emissions (DPOAEs) were divided into four groups. Group K was the control group. Group A was injected intramuscularly (i.m.) with amikacin 600 mg/kg/day between days 1-15. Group AB was given beta glucan gavage 1 mg/kg/day on days 0-15 and given amikacin 600 mg/kg/day i.m. on days 1-15. Group B was administered only beta glucan gavage, 1 mg/kg/day, on days 0-15. The DPOAEs were elicited in different frequency regions between 2,003 and 9,515 Hz, as distortion product diagrams (DPgrams), before and after the medication was administered, in all groups, on days 1, 5, 10, and 15.
  
  
  RESULTS
  No significant changes in the DPgrams were observed in group K. In group A, significant deterioration was observed at the 8,003 and 9,515 Hz frequencies on day 10, and at the 3,991, 4,557, 5,660, 6,726, 8,003, and 9,515 Hz frequencies on day 15. For group AB, statistically significant deterioration was observed at the 2,824, 8,003, and 9,515 Hz frequencies on day 15. The results for group B showed a significant improvement of hearing at the 2,378, 2,824, 3,363, and 3,991 Hz frequencies on day 1, at the 3,363, 3,991, and 8,003 Hz frequencies on day 10, and at the 8,003 Hz frequency on day 15.
  
  
  CONCLUSIONS
  This study suggests that amikacin-induced hearing loss in rats may be limited to some extent by concomitant use of beta glucan.
authors.affiliation: Department of Otorhinolaryngology, Inonu University Medical Faculty, Malatya, Turkey.
authors.forename: Tuba
authors.initials: T
authors.lastname: Bayindir
authors.forename: Aliye
authors.initials: A
authors.lastname: Filiz
authors.forename: Mustafa
authors.initials: M
authors.lastname: Iraz
authors.forename: Serdar
authors.initials: S
authors.lastname: Kaya
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tan
authors.forename: Mahmut Tayyar
authors.initials: MT
authors.lastname: Kalcioglu
keywords: Amikacin; Beta glucan; Otoacoustic emission measurement