title: Experimental study on topical treatment of diabetic skin ulcers with yi medicine "yi bu a jie" extract.
abstract: OBJECTIVE
  To study the role and mechanism of the Yi medicine, Yi Bu A Jie () extract, in topical treatment of diabetic skin ulcers, with a view to finding a breakthrough natural drug for the prevention and treatment of diabetic skin ulcers.
  
  
  METHODS
  A model of diabetic skin ulcers in Kunming mice was developed. Yi Bu A Jie was extracted in a Soxhlet extractor. Two different concentrations of the extract (0.005 mg/mL and 0.01 mg/mL) were applied to the wound of diabetic skin ulcers once every 3 days, and local skin appearance and histopathological changes were observed.
  
  
  RESULTS
  The shortest healing time was 25.25±2.06 day with a low concentration (P=0.0037 compared with the high concentration group, 33.14±2.21 day; P=0.0082 compared with control group, 28.21±2.14 days). The longest healing time was in the high concentration group (P=0.0025 compared with the control group). In both groups, a large number of inflammatory neutrophil cells were exuded during the experimental period. In the low concentration group, capillary-rich granulation tissue and actively growing fibroblasts appeared in the wound, while there was much necrotic tissue in the high concentration group.
  
  
  CONCLUSIONS
  Yi Bu A Jie extract has an inhibitory effect on diabetic skin ulcers in mice, and the low concentration is more suitable.
authors.affiliation: Department of Biology, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Lu
authors.forename: Ping
authors.initials: P
authors.lastname: Wan
authors.forename: Li-zhen
authors.initials: LZ
authors.lastname: Li
authors.forename: Mei-jun
authors.initials: MJ
authors.lastname: Zhao
authors.forename: Jia-yao
authors.initials: JY
authors.lastname: Hu
authors.forename: Yong-fang
authors.initials: YF
authors.lastname: Zhao
mesh_terms: D000287:Administration, Topical; D000818:Animals; D048909:Diabetes Complications / Q000188:drug therapy* / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D012883:Skin Ulcer / Q000188:drug therapy* / Q000473:pathology; D013997:Time Factors; D014020:Tissue Extracts / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D004364:Pharmaceutical Preparations; D014020:Tissue Extracts; C588020:yi bu a jie