title: Ca2+ Imaging as a tool to assess TRP channel function in murine distal nephrons.
abstract: Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca(2+) entry from the intratubular space into cells in response to external cues, TRP channels modulate water-electrolyte transport, thus determining functional properties of the renal tubule. In this chapter, we discuss technical aspects of using Ca(2+) imaging to monitor activity of TRP channels in situ, namely, in the freshly isolated distal nephrons, with a special emphasis on the mechanosensitive TRPV4 channel and its role in tubular flow sensing.
authors.affiliation: Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, Houston, TX, USA.
authors.forename: Mykola
authors.initials: M
authors.lastname: Mamenko
authors.forename: Oleg
authors.initials: O
authors.lastname: Zaika
authors.forename: Roger G
authors.initials: RG
authors.lastname: O'Neil
authors.forename: Oleh
authors.initials: O
authors.lastname: Pochynyuk
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D009399:Nephrons / Q000166:cytology / Q000378:metabolism*; D010539:Permeability; D051381:Rats; D013314:Stress, Mechanical; D050051:Transient Receptor Potential Channels / Q000378:metabolism*
chemical_list: D050051:Transient Receptor Potential Channels; D002118:Calcium