title: Human papillomavirus genotypes distribution in cervical samples from Uruguayan women.
abstract: Persistent infection with high-risk human papillomavirus (HPV) causes cervical preneoplasic lesions and invasive cervical cancer. This study evaluated the prevalence and distribution of HPV genotypes in cervical exfoliated cells from Uruguayan women. Five hundred sixty-eight cervical specimens were examined by PCR using MY09/11 primer set, and were genotyped by restriction enzyme digestion (RFLP). Some of the samples which remained undetermined were reanalyzed by PGMY PCR combined with reverse line blot hybridization. Overall, about 42% of samples were positive for HPV; 96% in high-grade squamous intraepithelial lesion, 66% in low-grade squamous intraepithelial lesion, 15% in atypical squamous cells of undetermined significance, and 19% in samples negative for intraepithelial lesion or malignancy. HPV 16 was the most commonly found genotype, followed by HPV 68 and 58. Within low risk-HPV genotypes 6, 61, and 11 were the most frequent. This is the first cross-sectional study, accounting for prevalence and genotype distribution of HPV in Uruguayan women.
authors.affiliation: Virology Department, Faculty of Science, University of the Republic, Montevideo, Uruguay.
authors.forename: Viviana
authors.initials: V
authors.lastname: Ramas
authors.forename: Santiago
authors.initials: S
authors.lastname: Mirazo
authors.forename: Sylvia
authors.initials: S
authors.lastname: Bonilla
authors.forename: Laura
authors.initials: L
authors.lastname: Mendoza
authors.forename: Olga
authors.initials: O
authors.lastname: Lago
authors.forename: Jorge
authors.initials: J
authors.lastname: Basiletti
authors.forename: Joaquin
authors.initials: J
authors.lastname: González
authors.forename: Maria Alejandra
authors.initials: MA
authors.lastname: Picconi
authors.forename: Juan
authors.initials: J
authors.lastname: Arbiza
mesh_terms: D000293:Adolescent; D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000821:virology; D002584:Cervix Uteri / Q000821:virology*; D016172:DNA Fingerprinting; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D017720:Molecular Epidemiology; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D014583:Uruguay / Q000453:epidemiology; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000821:virology
chemical_list: D004279:DNA, Viral