title: Functional analysis of a survivin-like gene in Bombyx mori.
abstract: The survivin (svv) gene is a newly discovered member of the inhibitors of apoptosis gene family. In recent years, svv has been confirmed to have an anti-apoptosis function and to play a critical role in cell division. We identified a survivin-like gene in the silkworm, Bombyx mori (Bm-svv). In this study, to gain insight into its function, a baculovirus expression system was used to express the Bm-svv gene in insect cell lines. The recombinant viruses were then used as a vector to transform insect cells, and cell activity was determined using the Cell Counting Kit-8 (CCK-8), which is usually employed for detecting mammalian cell number. The results indicated that the Bm-svv gene plays a role in the cell growth arrest or apoptosis induced by viruses. Furthermore, the CCK-8 kit is effective in determining the activity of insect cells.
authors.affiliation: Institute of Life Sciences, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China, tang11_23@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.forename: Haixiang
authors.initials: H
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Huiling
authors.initials: H
authors.lastname: Wu
authors.forename: Wenbing
authors.initials: W
authors.lastname: Wang