title: Expression and imprinting analysis of AK044800, a transcript from the Dlk1-Dio3 imprinted gene cluster during mouse embryogenesis.
abstract: Recent advances of induced pluripotent stem cells (iPSCs) has demonstrated that full development potential is closely related with the expression state of noncoding RNAs (ncRNAs) of the Dlk1-Dio3 imprinted gene cluster. However, few of them, especially the long noncoding RNAs (lncRNAs), have been characterized in detail. AK044800 is a transcript from the Dlk1-Dio3 imprinted region with little known information. This study reports original data on the expression pattern of AK044800 during embryogenesis. Expression analysis showed that AK044800 was specifically expressed in the brain at mid-gestation, E9.5 and E11.5. And at E15.5, its expression was mainly concentrated in the forebrain. In the late-gestation stage (E18.5), AK044800 expression was weaker in the brain and began to emerge in some other tissues during this period. Notably, the expression of AK044800 was biallelic in the brain, unlike other noncoding transcripts from this imprinted region. In addition, its expression was dependent on inbred mouse strains. This may be the first lncRNA that has been identified with a different expression between inbred mouse strains. This study may provide useful clues for further investigations of expression regulation and functions of lncRNAs of the Dlk1-Dio3 imprinted region.
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Science Park of Harbin Institute of Technology, Harbin, China.
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Han
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Yijun
authors.initials: Y
authors.lastname: Tian
authors.forename: Weili
authors.initials: W
authors.lastname: Yan
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018392:Genomic Imprinting; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007453:Iodide Peroxidase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D005810:Multigene Family; D039904:Pluripotent Stem Cells / Q000378:metabolism / Q000502:physiology*
chemical_list: D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; C117274:iodothyronine deiodinase type III; D007453:Iodide Peroxidase