title: Activation of the aryl hydrocarbon receptor sensitizes mice to nonalcoholic steatohepatitis by deactivating mitochondrial sirtuin deacetylase Sirt3.
abstract: Nonalcoholic steatohepatitis (NASH) is a liver disorder that still demands improved treatment. Understanding the pathogenesis of NASH will help to develop novel approaches to prevent or treat this disease. In this study, we revealed a novel function of the aryl hydrocarbon receptor (AhR) in NASH. Transgenic or pharmacological activation of AhR heightened animal sensitivity to NASH induced by the methionine- and choline-deficient (MCD) diet, which was reasoned to be due to increased hepatic steatosis, production of reactive oxygen species (ROS), and lipid peroxidation. Mechanistically, the increased ROS production in AhR-activated mouse liver was likely a result of a lower superoxide dismutase 2 (SOD2) activity and compromised clearance of ROS. Activation of AhR induced tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly(ADP-ribose) polymerase (TiPARP) gene expression, depleted NAD(+), deactivated the mitochondrial sirtuin deacetylase 3 (Sirt3), increased SOD2 acetylation, and thereby decreased SOD2 activity. We also showed that Sirt3 ablation sensitized mice to NASH, whereas adenoviral overexpression of Sirt3 alleviated the NASH phenotype in AhR-transgenic mice. We conclude that activation of AhR sensitizes mice to NASH by facilitating both the "first hit" of steatosis and the "second hit" of oxidative stress. Our results suggest that the use of AhR antagonists might be a viable approach to prevent and treat NASH. Manipulation of the expression or activity of Sirt3 may also represent a novel approach to manage NASH.
authors.affiliation: Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA, USA.
authors.forename: Jinhan
authors.initials: J
authors.lastname: He
authors.forename: Bingfang
authors.initials: B
authors.lastname: Hu
authors.forename: Xiongjie
authors.initials: X
authors.lastname: Shi
authors.forename: Eric R
authors.initials: ER
authors.lastname: Weidert
authors.forename: Peipei
authors.initials: P
authors.lastname: Lu
authors.forename: Meishu
authors.initials: M
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Eric E
authors.initials: EE
authors.lastname: Kelley
authors.forename: Wen
authors.initials: W
authors.lastname: Xie
mesh_terms: D000107:Acetylation; D000818:Animals; D002796:Choline Deficiency / Q000150:complications; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism*; D005526:Food, Formulated; D015870:Gene Expression; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology; D008715:Methionine / Q000172:deficiency; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008930:Mitochondria, Liver / Q000201:enzymology; D065626:Non-alcoholic Fatty Liver Disease; D018384:Oxidative Stress; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D056566:Sirtuin 3 / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D008715:Methionine; D000072317:Polychlorinated Dibenzodioxins; D017382:Reactive Oxygen Species; D018336:Receptors, Aryl Hydrocarbon; D056566:Sirtuin 3; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C525046:Sirt3 protein, mouse