title: Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells.
abstract: We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2'-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.
authors.affiliation: Department of Radiological Sciences, Jeonju University, Jeonju, 560-759, Korea.
authors.forename: Jiwon
authors.initials: J
authors.lastname: Choi
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Kang
authors.forename: Sung Zoo
authors.initials: SZ
authors.lastname: Kim
authors.forename: Tae Oh
authors.initials: TO
authors.lastname: Kwon
authors.forename: Seung-Il
authors.initials: SI
authors.lastname: Jeong
authors.forename: Seon Il
authors.initials: SI
authors.lastname: Jang
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005609:Free Radicals / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D044949:Kaempferols / Q000302:isolation & purification / Q000494:pharmacology*; D031625:Morus; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D018515:Plant Leaves
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D044949:Kaempferols; C001579:astragalin