title: Identification of the immunogenic spore and vegetative proteins of Bacillus anthracis vaccine strain A16R.
abstract: Immunoproteomics was used to screen the immunogenic spore and vegetative proteins of Bacillus anthracis vaccine strain A16R. The spore and vegetative proteins were separated by 2D gel electrophoresis and transferred to polyvinylidene difluoride membranes, and then western blotting was performed with rabbit immune serum against B.anthracis live spores. Immunogenic spots were cut and digested by trypsin. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry was performed to identify the proteins. As a result, 11 and 45 immunogenic proteins were identified in the spores and vegetative cells, respectively; 26 of which have not been reported previously. To verify their immunogenicity, 12 of the identified proteins were selected to be expressed, and the immune sera from the mice vaccinated by the 12 expressed proteins, except BA0887, had a specific western blot band with the A16R whole cellular lytic proteins. Some of these immunogenic proteins might be used as novel vaccine candidates themselves or for enhancing the protective efficacy of a protective-antigen-based vaccine.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, People's Republic of China.
authors.forename: Xiankai
authors.initials: X
authors.lastname: Liu
authors.forename: Dongshu
authors.initials: D
authors.lastname: Wang
authors.forename: Jingxiao
authors.initials: J
authors.lastname: Ren
authors.forename: Chao
authors.initials: C
authors.lastname: Tong
authors.forename: Erling
authors.initials: E
authors.lastname: Feng
authors.forename: Xuefang
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Hengliang
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D022122:Anthrax Vaccines / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001408:Bacillus anthracis / Q000276:immunology* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000276:immunology*; D019295:Computational Biology / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D040901:Proteomics; D011817:Rabbits; D015203:Reproducibility of Results; D013171:Spores, Bacterial / Q000276:immunology*
chemical_list: D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera