title: Neurocysticercosis: HP10 antigen detection is useful for the follow-up of the severe patients.
abstract: BACKGROUND
  The most severe clinical form of neurocysticercosis (NC) occurs when cysticerci are located in the subarachnoid space at the base of the brain (SaB). The diagnosis, monitoring and treatment of NC-SaB, constitutes a severe clinical challenge. Herein we evaluate the potential of the HP10 antigen detection enzyme-linked immunosorbent assay (HP10 Ag-ELISA) in the long term follow-up of NC-SaB cases. Assay performance was compared with that of Magnetic Resonance Imaging (MRI). In addition, the robustness of the HP10 Ag-ELISA was evaluated independently at two different institutions.
  
  
  RESULTS
  A double-blind prospective cohort trial was conducted involving 38 NC-SaB cases and a total of 108 paired serum and cerebrospinal fluid (CSF) samples taken at intervals of 4 to 8 months for up to 43 months. At each medical visit, results of sera and CSF HP10 Ag-ELISA and MRI obtained at last visit were compared and their accuracy was evaluated retrospectively, considering radiological evolution between appointments. In the long-term follow-up study, HP10 Ag-ELISA had a better agreement than MRI with retrospective radiological evaluation. High reproducibility of HP10 Ag-ELISA between laboratories was also demonstrated.
  
  
  CONCLUSIONS
  Results reported in this study establish for the first time the usefulness of the comparatively low cost HP10 Ag-ELISA for long term follow-up of NC-SaB patients.
authors.affiliation: Unidad Periférica, Instituto de Investigaciones Biomédicas, UNAM / Instituto Nacional de Neurología y Neurocirugía, Colonia la Fama, Delegación Tlalpan, México DF, México. afleury@biomedicas.unam.mx
authors.forename: Agnès
authors.initials: A
authors.lastname: Fleury
authors.forename: Esperanza
authors.initials: E
authors.lastname: Garcia
authors.forename: Marisela
authors.initials: M
authors.lastname: Hernández
authors.forename: Roger
authors.initials: R
authors.lastname: Carrillo
authors.forename: Tzipe
authors.initials: T
authors.lastname: Govezensky
authors.forename: Gladis
authors.initials: G
authors.lastname: Fragoso
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
authors.forename: Leslie J S
authors.initials: LJ
authors.lastname: Harrison
authors.forename: R Michael Evans
authors.initials: RM
authors.lastname: Parkhouse
mesh_terms: D000328:Adult; D000368:Aged; D000947:Antigens, Helminth / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D001921:Brain / Q000000981:diagnostic imaging; D019411:Clinical Laboratory Techniques / Q000191:economics / Q000379:methods*; D015331:Cohort Studies; D016903:Drug Monitoring / Q000191:economics / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000188:drug therapy*; D010274:Parasitology / Q000191:economics / Q000379:methods*; D011446:Prospective Studies; D011859:Radiography; D016032:Randomized Controlled Trials as Topic
chemical_list: D000947:Antigens, Helminth; D015415:Biomarkers