title: [Effect of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae].
abstract: OBJECTIVE
  To determine the effects of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae (SCAP) in vitro.
  
  
  METHODS
  SCAP were isolated and cultured respectively in alpha minimum essential medium (α-MEM) or α-MEM containing 1.8 mmol/L KH2PO4. Alkaline phosphatase (ALP) activity, alizarin red staining, real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine the odonto and osteogenic potential of SCAP in the two media.
  
  
  RESULTS
  SCAP cultured in α-MEM containing 1.8 mmol/L KH2PO4 exhibited a higher ALP activity [(0.370 ± 0.013) Sigma unit×min(-1)×mg(-1)] at day 3 than control group [(0.285 ± 0.008) Sigma unit×min(-1)×mg(-1)] and KH2PO4-treated SCAP formed more calcified nodules at day 5 [(0.539 ± 0.007) µg/g] and day 7 [(1.617 ± 0.042) µg/g] than those in normal medium [(0.138 ± 0.037) µg/g, P < 0.01]. The expression of odonto- and osteogenic markers were significantly up-regulated after the stimulation of KH2PO4 at day 3 and 7 respectively, as compared with control group.
  
  
  CONCLUSIONS
  1.8 mmol/L KH2PO4 can promote the odonto and osteogenic differentiation potential of human SCAP.
authors.affiliation: Department of Endodontics, Nanjing Medical University, Nanjing, China.
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Jin-tao
authors.initials: JT
authors.lastname: Wu
authors.forename: Zi-lu
authors.initials: ZL
authors.lastname: Wang
authors.forename: Yang-yu
authors.initials: YY
authors.lastname: Zheng
authors.forename: Guang-dong
authors.initials: GD
authors.lastname: Zhang
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Yu
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology; D015675:Osteocalcin / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D017680:Potassium Compounds / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D010710:Phosphates; D010750:Phosphoproteins; D017680:Potassium Compounds; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C013216:potassium phosphate