title: Intracellular degradation of multilabeled poly(ethylene imine)-mesoporous silica-silica nanoparticles: implications for drug release.
abstract: Mesoporous silica nanoparticles, MSNs, have emerged as an interesting carrier for drugs in vitro and in vivo. The particles are typically used in a surface functionalized form, where functional silanes or other covalently linked surface functions are used to provide anchoring sites for additional functionalities like targeting groups, imaging agents, and drugs. Here, we report results related to extra- and intracellular degradation of silica nanoparticles using multilabeled nonporous silica core-mesoporous silica shell-surface hyperbranched poly(ethylene imine) shell nanoparticles as model particles. Different fluorophores have been selectively covalently linked to different regions of the particles in order to study the particle degradation in detail under in vitro conditions in human SAOS-2 cells. A novel, quantitative method for nanoparticle degradation evaluation based on confocal fluorescence microscopy is applied. Our results suggest that the core-shell-shell MSNs degrade at a higher rate inside cells as compared to outside cells, which is of high importance for further application of this class of drug carriers.
authors.affiliation: Laboratory for Physical Chemistry, Åbo Akademi University, Porthansgatan 3-5, FI-20500 Turku, Finland.
authors.forename: Lotta
authors.initials: L
authors.lastname: Bergman
authors.forename: Pasi
authors.initials: P
authors.lastname: Kankaanpää
authors.forename: Silja
authors.initials: S
authors.lastname: Tiitta
authors.forename: Alain
authors.initials: A
authors.lastname: Duchanoy
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Jyrki
authors.initials: J
authors.lastname: Heino
authors.forename: Mika
authors.initials: M
authors.lastname: Lindén
mesh_terms: D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006801:Humans; D007097:Imines / Q000737:chemistry* / Q000493:pharmacokinetics; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D011095:Polyethylenes / Q000737:chemistry* / Q000493:pharmacokinetics; D012822:Silicon Dioxide / Q000737:chemistry* / Q000493:pharmacokinetics; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D007097:Imines; D011095:Polyethylenes; C505405:poly(ethylene imine); D012822:Silicon Dioxide