title: Lessons we learned from high-throughput and top-down systems biology analyses about glioma stem cells.
abstract: A growing body of evidence suggests that glioma stem cells (GSCs) account for tumor initiation, therapy resistance, and the subsequent regrowth of gliomas. Thus, continuous efforts have been undertaken to further characterize this subpopulation of less differentiated tumor cells. Although we are able to enrich GSCs, we still lack a comprehensive understanding of GSC phenotypes and behavior. The advent of high-throughput technologies raised hope that incorporation of these newly developed platforms would help to tackle such questions. Since then a couple of comparative genome-, transcriptome- and proteome-wide studies on GSCs have been conducted giving new insights in GSC biology. However, lessons had to be learned in designing high-throughput experiments and some of the resulting conclusions fell short of expectations because they were performed on only a few GSC lines or at one molecular level instead of an integrative poly-omics approach. Despite these shortcomings, our knowledge of GSC biology has markedly expanded due to a number of survival-associated biomarkers as well as glioma-relevant signaling pathways and therapeutic targets being identified. In this article we review recent findings obtained by comparative high-throughput analyses of GSCs. We further summarize fundamental concepts of systems biology as well as its applications for glioma stem cell research.
authors.forename: Andreas
authors.initials: A
authors.lastname: Mock
authors.forename: Sara
authors.initials: S
authors.lastname: Chiblak
authors.affiliation: Experimentelle Neurochirurgie, Neurochirurgische Universitätsklinik, INF 400, 69120 Heidelberg, Germany. H.Mende@med.uni-heidelberg.de.
authors.forename: Christel
authors.initials: C
authors.lastname: Herold-Mende
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D005910:Glioma / Q000473:pathology*; D057166:High-Throughput Screening Assays; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology*; D049490:Systems Biology