title: Inhibitory effects of traditional herbal formula pyungwi-san on inflammatory response in vitro and in vivo.
abstract: Pyungwi-san (PWS) is a traditional basic herbal formula. We investigated the effects of PWS on induction of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), pro-inflammatory cytokines (interleukin-6 (IL-6) and tumor necrosis factor- α (TNF- α )) and nuclear factor-kappa B (NF- κ B) as well as mitogen-activated protein kinases (MAPKs) in lipopolysaccharide-(LPS-) induced Raw 264.7 cells and on paw edema in rats. Treatment with PWS (0.5, 0.75, and 1 mg/mL) resulted in inhibited levels of expression of LPS-induced COX-2, iNOS, NF- κ B, and MAPKs as well as production of prostaglandin E2 (PGE2), nitric oxide (NO), IL-6, and TNF- α induced by LPS. Our results demonstrate that PWS possesses anti-inflammatory activities via decreasing production of pro-inflammatory mediators through suppression of the signaling pathways of NF- κ B and MAPKs in LPS-induced macrophage cells. More importantly, results of the carrageenan-(CA-) induced paw edema demonstrate an anti-edema effect of PWS. In addition, it is considered that PWS also inhibits the acute edematous inflammations through suppression of mast cell degranulations and inflammatory mediators, including COX-2, iNOS and TNF- α . Thus, our findings may provide scientific evidence to explain the anti-inflammatory properties of PWS in vitro and in vivo.
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Cha
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Jung
authors.forename: Jae Yup
authors.initials: JY
authors.lastname: Jung
authors.forename: Jong Rok
authors.initials: JR
authors.lastname: Lee
authors.forename: Il Je
authors.initials: IJ
authors.lastname: Cho
authors.forename: Sae Kwang
authors.initials: SK
authors.lastname: Ku
authors.forename: Sung Hui
authors.initials: SH
authors.lastname: Byun
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Ahn
authors.forename: Chul Won
authors.initials: CW
authors.lastname: Lee
authors.forename: Sang Chan
authors.initials: SC
authors.lastname: Kim
authors.forename: Won G
authors.initials: WG
authors.lastname: An