title: Realistic representation of Bacillus subtilis biofilms architecture using combined microscopy (CLSM, ESEM and FESEM).
abstract: In this contribution, we used a set of microscopic techniques including confocal laser scanning microscopy (CLSM), environmental scanning electron microscopy (ESEM) and field emission scanning electron microscopy (FESEM) to analyze the three-dimensional spatial arrangement of cells and their surrounding matrix in Bacillus subtilis biofilm. The combination of the different techniques enabled a deeper and realistic deciphering of biofilm architecture by providing the opportunity to overcome the limits of each single technique.
authors.affiliation: INRA, UMR 1319 Micalis, F-78350 Jouy-en-Josas, France.
authors.forename: A
authors.initials: A
authors.lastname: Bridier
authors.forename: T
authors.initials: T
authors.lastname: Meylheuc
authors.forename: R
authors.initials: R
authors.lastname: Briandet
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D018441:Biofilms / Q000254:growth & development*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning