title: Assessment of the argyrophilic nucleolar organizer region area/nucleus ratio in ovarian serous epithelial adenomas, borderline tumors and cancers.
abstract: BACKGROUND
  There is a need to assess the value of the novel potentially useful biomarkers in ovarian tumors. The aim of study was to assess the value of sAgNOR analysis in ovarian serous epithelial tumors.
  
  
  METHODS
  The analysis was performed in ovaries from 113 patients treated operatively due to serous ovarian tumors (30 adenomas, 14 borderline tumors and 69 cancers). After silver staining of paraffin specimens from surgery, sAgNOR in tumor cells was analyzed. Additionally, the value of the argyrophilic nucleolar organizer region area/nucleus ratio (sAgNOR) in the prediction of disease-free survival (DFS) and overall survival (OS) in 52 patients with serous ovarian cancer with complete follow-ups in November 2009 was evaluated. Age, grading, radicality of surgery and FIGO staging were analyzed as additional factors.
  
  
  RESULTS
  SAGNOR IN ADENOMAS, BORDERLINE TUMORS AND CANCERS WAS IN THE FOLLOWING RANGES: (0.73 ±0.23) × 10(6), (0.81 ±0.18) × 10(6) and (0.96 ±0.33) × 10(6) [AgNOR/cm(2)] respectively. In cancers from G1 to G3 sAgNOR was (1.02 ±0.32) × 10(6) (G1), (0.98 ±0.37) × 10(6) (G2) and (0.82 ±0.24) × 10(6) (G3) [AgNOR/cm(2)] respectively. In univariate analysis, but not in multivariate analysis, staging negatively correlated with better DFS and OS. sAgNOR, age of patients, grading and radicality of surgery were not associated with DFS or OS in either univariate or multivariate analysis.
  
  
  CONCLUSIONS
  sAgNOR analysis is not sufficient to precisely characterize cellular kinetics in serous ovarian tumors, and the analysis of sAgNOR, mAgNOR and pAgNOR should be performed commonly. The prognostic significance of sAgNOR in patients with serous ovarian cancer was not proven.
authors.affiliation: Department of Radiotherapy, Chair of Oncology, Medical University of Lodz, Poland ; Department of Radiotherapy, Regional Cancer Center, Copernicus Memorial Hospital of Lodz, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Gottwald
authors.forename: Marian
authors.initials: M
authors.lastname: Danilewicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Suzin
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Wagrowska-Danilewicz
authors.forename: Michal
authors.initials: M
authors.lastname: Spych
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Tylinski
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Topczewska-Tylinska
authors.forename: Janusz
authors.initials: J
authors.lastname: Piekarski
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kazmierczak-Lukaszewicz
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Cialkowska-Rysz
keywords: argyrophilic; nucleolar organizer regions; ovarian tumors; serous