title: Chemopreventive effects of the juice of Vitis coignetiae Pulliat on two-stage mouse skin carcinogenesis.
abstract: Our study revealed the inhibitory effect of Vitis coignetiae Pulliat, known as Yamabudo in Japan, at the stages of multi-step carcinogenesis. The juice of Vitis coignetiae (Y-grape juice) was antimutagenic toward dimethylbenzo[a]anthracene (DMBA), aflatoxin B1, and benzo[a]pyrene in the Ames test. The Y-grape juice was also antigenotoxic in the micronucleus test using HepG2 cells toward DMBA and aflatoxin B1. Topical and oral administration of the Y-grape juice to mice inhibited the induction of inflammation of 12-O-tetradecanoylphorbol-13-acetate (TPA). Topical and oral administration of the Y-grape juice significantly decreased the incidence and mean number of tumors in mice skin with the 2-stage tumorigenesis protocol. To elucidate the mechanisms underlying the antiinflammatory and antitumor promotion activity of the Y-grape juice, the effect of Y-grape juice on cyclooxygenase-2 (COX-2) activity in mouse ear treated with TPA was studied. Both topical and oral application of the Y-grape juice inhibited the TPA-induced increase in COX-2 activity. Caftaric acid, isolated and identified from the Y-grape juice, was antimutagenic toward DMBA and prevented TPA-induced inflammation in mice, suggesting caftaric acid participates in chemopreventive effect/activities of Y-grape juice.
authors.affiliation: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, Japan. arimoto@cc.okayama-u.ac.jp
authors.forename: Sakae
authors.initials: S
authors.lastname: Arimoto-Kobayashi
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuta
authors.initials: Y
authors.lastname: Yuhara
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kamiya
authors.forename: Tomoe
authors.initials: T
authors.lastname: Negishi
authors.forename: Goro
authors.initials: G
authors.lastname: Okamoto
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors; D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents; D016588:Anticarcinogenic Agents / Q000008:administration & dosage*; D016587:Antimutagenic Agents; D000975:Antioxidants; D002273:Carcinogens; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000008:administration & dosage; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005638:Fruit / Q000737:chemistry; D051379:Mice; D018632:Mice, Inbred SENCAR; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D000975:Antioxidants; D002273:Carcinogens; D052246:Cyclooxygenase 2 Inhibitors; D010636:Phenols; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene; D051546:Cyclooxygenase 2; D013755:Tetradecanoylphorbol Acetate; C515101:caftaric acid