title: [The new technologies for the analytical examination of the embryonic metabolome and its prospects].
abstract: OBJECTIVE
  To review current technologies for the analytical examination of the embryonic metabolome and its perspectives.
  
  
  METHODS
  Review article.
  
  
  METHODS
  Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and University Hospital, Brno, Department of Biochemistry, Faculty of Science and CEITEC, Masaryk University, Brno.
  
  
  RESULTS
  Nowadays, very sensitive analytical technologies are available. They enable exact measurement of various molecules - products of embryo metabolism during first days of cultivation. The capillary electrophoresis is one of promising method. Recent studies analysed metabolic differences between embryos that result in a pregnancy and those that do not. Amino acid levels, glucose or pyruvate in the embryo culture media were analysed most frequently. However, results of these studies are ambiguous.
  
  
  CONCLUSIONS
  The capillary electrophoresis with contactless conductivity detection may provide a useful data of the embryonic metabolome. A comprehensive analysis of the used culture medium may represent a valuable adjunct to morphological criteria for enhanced rates of implantation and delivery.
authors.affiliation: Gynekologicko-porodnicka klinika LF MU a FN Brno. icrha@fnbrno.cz
authors.forename: I
authors.initials: I
authors.lastname: Crha
authors.forename: A
authors.initials: A
authors.lastname: Mádr
authors.forename: J
authors.initials: J
authors.lastname: Musilová
authors.forename: Z
authors.initials: Z
authors.lastname: Glatz
authors.forename: J
authors.initials: J
authors.lastname: Záková
authors.forename: P
authors.initials: P
authors.lastname: Ventruba
mesh_terms: D002623:Chemistry Techniques, Analytical; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D006801:Humans; D055442:Metabolome; D011247:Pregnancy