title: Multicolor stimulated Raman scattering (SRS) microscopy.
abstract: Stimulated Raman scattering (SRS) microscopy has opened up a wide range of biochemical imaging applications by probing a particular Raman-active molecule vibrational mode in the specimen. However, the original implementation with picosecond pulse excitation can only realize rapid chemical mapping with a single Raman band. Here we present a novel SRS microscopic technique using a grating-based pulse shaper for excitation and a grating-based spectrograph for detection to achieve simultaneous multicolor SRS imaging with high sensitivity and high acquisition speeds. In particular, we used linear combination of the measured CH2and CH3stretching signals to map the distributions of protein and lipid contents simultaneously.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States.
authors.forename: Fa-Ke
authors.initials: FK
authors.lastname: Lu
authors.forename: Minbiao
authors.initials: M
authors.lastname: Ji
authors.forename: Dan
authors.initials: D
authors.lastname: Fu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ni
authors.forename: Christian W
authors.initials: CW
authors.lastname: Freudiger
authors.forename: Gary
authors.initials: G
authors.lastname: Holtom
authors.forename: X Sunney
authors.initials: XS
authors.lastname: Xie
keywords: coherent Raman scattering; grating; lipid; lock-in; mouse skin; multicolor; poly(methyl methacrylate); polystyrene; protein; pulse shaper; stimulated Raman scattering