title: Evaluation of glucosinolate variation in a collection of turnip (Brassica rapa) germplasm by the analysis of intact and desulfo glucosinolates.
abstract: Glucosinolates (GLS) are secondary metabolites occurring in cruciferous species. These compounds are important for plant defense, human health, and the characteristic flavor of Brassica vegetables. In this study, the GLS in tubers from a collection of 48 turnip ( Brassica rapa ) accessions from different geographic origin were analyzed. Two different methods were used: desulfo GLS were analyzed by high-performance liquid chromatography with a photodiode array detector, and intact GLS were analyzed by accurate mass liquid chromatography-mass spectrometry. For most GLS, desulfo and intact signals correlated well, and the analytical reproducibility for individual GLS was similar for both methods. A total of 11 different GLS was monitored in the turnip tubers, through both intact and desulfo GLS analysis methods. Four clusters of accessions could be clearly distinguished based on GLS composition of the turnip tuber. Clustering based on tuber GLS differed markedly from a previously published clustering based on leaf GLS.
authors.affiliation: National Institute of Horticultural and Herbal Science, Department of Horticultural Crop Research, Rural Development Administration, Suwon 440-706, Korea.
authors.forename: Jun Gu
authors.initials: JG
authors.lastname: Lee
authors.forename: Guusje
authors.initials: G
authors.lastname: Bonnema
authors.forename: Ningwen
authors.initials: N
authors.lastname: Zhang
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Kwak
authors.forename: Ric C H
authors.initials: RC
authors.lastname: de Vos
authors.forename: Jules
authors.initials: J
authors.lastname: Beekwilder
mesh_terms: D018070:Biological Specimen Banks; D029688:Brassica napus / Q000737:chemistry* / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D005961:Glucosinolates / Q000032:analysis* / Q000235:genetics*; D013058:Mass Spectrometry; D035281:Plant Tubers / Q000737:chemistry; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D013455:Sulfur / Q000737:chemistry
chemical_list: D005961:Glucosinolates; D013455:Sulfur