title: LC-MS/MS analysis of chitooligosaccharides.
abstract: Chitooligosaccharides with degrees of polymerization (DPs) 2-12 were successfully separated and characterized using LC-MS/MS analysis with an amine column and a quadrupole ion trap analyzer. Major MS peaks for each chromatographic peak originated from deacetylated chitooligosaccharides while N-acetylated chitooligosaccharides were observed as minor peaks. The ratio of acetylation per glucosamine unit for each chitooligomer was higher for oligomers with higher DPs. The MS/MS spectra of chitooligosaccharides were used to characterize the chitooligosaccharide fragmentation patterns. To the best of our knowledge, this is the first report of on-line LC-MS/MS analysis of chitooligosaccharides with DP higher than 6.
authors.affiliation: Department of Chemistry, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon 305-764, South Korea.
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Kim
authors.forename: Jinhee
authors.initials: J
authors.lastname: Kim
authors.forename: Jangmi
authors.initials: J
authors.lastname: Hong
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Sehwan
authors.initials: S
authors.lastname: Park
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Jeongkwon
authors.initials: J
authors.lastname: Kim
mesh_terms: D000107:Acetylation; D048271:Chitosan / Q000737:chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*; D009862:Online Systems; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D009844:Oligosaccharides; D048271:Chitosan