title: Crystallization and preliminary X-ray crystallographic studies of DnaJ from Streptococcus pneumoniae.
abstract: DnaJ, cooperating with DnaK and GrpE, promotes the folding of unfolded hydrophobic polypeptides, dissociates protein complexes and translocates protein across membranes. Additionally, DnaJ from Streptococcus pneumoniae (SpDnaJ) is involved in the infectious disease process and is being developed as a potential vaccine to prevent bacterial infection. Here the expression, purification, crystallization and preliminary crystallographic analysis of SpDnaJ are reported. The crystals belong to space groups I222 or I2₁2₁2₁ and the diffraction resolution is 3.0 Å with unit-cell parameters a=47.68, b=104.45, c=234.57 Å. The crystal most likely contains one molecule in the asymmetric unit, with a VM value of 3.24 Å3 Da(-1) and a solvent content of 62.1%.
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Disease, Chongqing Medical University, YiXueYuanlu-1, Chongqing 400016, People's Republic of China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Siqiang
authors.initials: S
authors.lastname: Niu
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Shaocheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Guo
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Ailong
authors.initials: A
authors.lastname: Huang
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.forename: Deqiang
authors.initials: D
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D050956:HSP40 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D050956:HSP40 Heat-Shock Proteins; D011994:Recombinant Proteins
keywords: DnaJ; Streptococcus pneumoniae