title: Attachment of nucleosides and other linkers to solid-phase supports for oligonucleotide synthesis.
abstract: Specific step-by-step instructions for conversion of 5'-O-(4,4'-dimethoxytrityl)- and base-protected nucleosides and other mono-O-(4,4'-dimethoxytrityl)-protected diols to their hemisuccinate esters and their coupling to CPG (controlled-pore glass) supports bearing aminopropyl or long chain aminoalkyl groups are presented. Additional guidelines are provided for selecting a coupling protocol and performing in-process control.
authors.affiliation: AM Chemicals LLC, Oceanside, California.
authors.forename: Andrei P
authors.initials: AP
authors.lastname: Guzaev
authors.affiliation: University of Calgary, Calgary, Alberta, Canada.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Pon
mesh_terms: D005898:Glass / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
chemical_list: D009705:Nucleosides; D009841:Oligonucleotides