title: Histomorphological and immunofluorescence evaluation of clear corneal incisions after microcoaxial phacoemulsification with 2.2 mm and 1.8 mm systems.
abstract: OBJECTIVE
  To compare changes in the incision's histomorphology and denaturation of collagen I in rabbit eyes having microcoaxial phacoemulsification through 2.2 mm and 1.8 mm incision-compatible systems.
  
  
  METHODS
  Randomized experimental trial.
  
  
  METHODS
  Iladevi Cataract & IOL Research Centre, Ahmedabad, India.
  
  
  METHODS
  Thirty rabbit eyes were randomized into Group 1 (microcoaxial phacoemulsification through 2.2 mm incisions using Infiniti system [torsional ultrasound]) and Group 2 (microcoaxial phacoemulsification through 1.8 mm incisions using Stellaris system [longitudinal ultrasound]). Each group was then divided into 3 subgroups of 5 eyes each based on 1 of the 3 intervention options: phacoemulsification only, intraocular lens (IOL) insertion only, and phacoemulsification with IOL insertion. Left eyes were randomized for microcoaxial phacoemulsification, and right eyes were treated as controls.
  
  
  RESULTS
  After phacoemulsification, eyes in Group 1 showed loss of epithelium at the roof of the incisions and Descemet membrane detachment at the floor of the incisions. These findings did not change after IOL insertion. After phacoemulsification, eyes in Group 2 showed loss of epithelium, but Descemet membrane remained attached. There was a longitudinal split in the incision's stroma in the direction of internal entry. The stromal damage increased after IOL implantation. Immunofluorescence studies showed no obvious irregularities in the arrangement of collagen I in either group. A dot blot analysis showed significant denaturation of collagen I in Group 2.
  
  
  CONCLUSIONS
  The histomorphology of the 2.2 mm system incision showed localized Descemet membrane detachment and endothelial cell loss. The 1.8 mm system incision showed exaggerated stromal damage after IOL insertion.
authors.affiliation: Iladevi Cataract & IOL Research Centre, Ahmedabad, India. icirc@abhayvasavada.com
authors.forename: Abhay R
authors.initials: AR
authors.lastname: Vasavada
authors.forename: Kaid
authors.initials: K
authors.lastname: Johar
authors.forename: Mamidipudi R
authors.initials: MR
authors.lastname: Praveen
authors.forename: Viraj A
authors.initials: VA
authors.lastname: Vasavada
authors.forename: Anshul I
authors.initials: AI
authors.lastname: Arora
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D003315:Cornea / Q000378:metabolism / Q000473:pathology* / Q000601:surgery*; D019084:Fluorescent Antibody Technique, Indirect; D019654:Lens Implantation, Intraocular; D008856:Microscopy, Fluorescence; D008866:Microsurgery / Q000379:methods*; D018918:Phacoemulsification / Q000379:methods*; D011817:Rabbits; D014945:Wound Healing
chemical_list: D024042:Collagen Type I