title: Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex.
abstract: METHODS
  Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients.
  
  
  RESULTS
  The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome.
  
  
  CONCLUSIONS
  Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.
authors.affiliation: Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of São Paulo School of Medicine, FMUSP, São Paulo, Brazil. keity@usp.br
authors.forename: Keity Souza
authors.initials: KS
authors.lastname: Santos
authors.forename: Gabriele
authors.initials: G
authors.lastname: Gadermaier
authors.forename: Eva
authors.initials: E
authors.lastname: Vejvar
authors.forename: Helen Andrade
authors.initials: HA
authors.lastname: Arcuri
authors.forename: Clovis Eduardo
authors.initials: CE
authors.lastname: Galvão
authors.forename: Ariana Campos
authors.initials: AC
authors.lastname: Yang
authors.forename: Virgínia Maria Ferreira
authors.initials: VM
authors.lastname: Resende
authors.forename: Carlo de Oliveira
authors.initials: Cde O
authors.lastname: Martins
authors.forename: Martin
authors.initials: M
authors.lastname: Himly
authors.forename: Adriano
authors.initials: A
authors.lastname: Mari
authors.forename: Marina
authors.initials: M
authors.lastname: Liso
authors.forename: Debora
authors.initials: D
authors.lastname: Pomponi
authors.forename: Heimo
authors.initials: H
authors.lastname: Breiteneder
authors.forename: Stefan
authors.initials: S
authors.lastname: Wagner
authors.forename: Jorge
authors.initials: J
authors.lastname: Kalil
authors.forename: Fátima
authors.initials: F
authors.lastname: Ferreira
authors.forename: Fábio Fernandes Morato
authors.initials: FF
authors.lastname: Castro
mesh_terms: D000328:Adult; D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000235:genetics* / Q000276:immunology*; D001938:Brazil; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007073:Immunoglobulin E / Q000276:immunology; D007840:Latex / Q000276:immunology; D020315:Latex Hypersensitivity / Q000097:blood / Q000276:immunology; D008297:Male; D002365:Manihot / Q000737:chemistry* / Q000276:immunology; D008875:Middle Aged; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D007106:Immune Sera; D007840:Latex; C585943:Man e 5 allergen, Manihot esculenta; D010940:Plant Proteins; D011994:Recombinant Proteins; C101800:allergen Hev b 5; D007073:Immunoglobulin E