title: Determination of the variations in levels of phenolic compounds in soybean (Glycine max Merr.) sprouts infected by anthracnose (Colletotrichum gloeosporioides).
abstract: BACKGROUND
  Soybean sprouts (Kongnamool) are one of the most popular and nutritive traditional vegetables in East Asia. Anthracnose caused by Colletotrichum gloeosporioides is one of the most serious diseases of soybean sprouts. In order to obtain basic information for breeding and/or selecting soybean genotypes with increased natural defense against anthracnose, phenolic compounds were profiled for healthy and infected soybean (Glycine max Merr.) sprouts by using high-performance liquid chromatography coupled with tandem mass spectrometry.
  
  
  RESULTS
  Tryptophan and eight phenolic compounds (daidzin, genistin, malonyldaidzin, malonylgenistin, daidzein, glycitein, genistein and coumestrol) were determined from healthy and inoculated sprouts. Total identified phenolic content was 40.02 ± 0.03 mg kg⁻¹, 99.4% of which was isoflavones.
  
  
  CONCLUSIONS
  The monitoring suggested that de novo induced glycitein appeared to act as a phytoalexin in the defence mechanism of the soybean sprouts against C. gloeosporioides, and constitutively formed seven phenolic components that functioned as phytoanticipins in the diseased soybean sprouts.
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jung Han
authors.initials: JH
authors.lastname: Lee
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Jeong
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Cho
authors.forename: Semin
authors.initials: S
authors.lastname: Park
authors.forename: Yun-Hi
authors.initials: YH
authors.lastname: Kim
authors.forename: Dong Won
authors.initials: DW
authors.lastname: Bae
authors.forename: Jong Il
authors.initials: JI
authors.lastname: Chung
authors.forename: Youn-Sig
authors.initials: YS
authors.lastname: Kwak
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Jeong
authors.forename: Soo-Chul
authors.initials: SC
authors.lastname: Park
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Jin
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
mesh_terms: D000935:Antifungal Agents / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D020231:Colletotrichum / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology; D018525:Germination; D005960:Glucosides / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D007529:Isoflavones / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development / Q000276:immunology; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D057865:Plant Immunity; D056910:Republic of Korea; D036226:Seedlings / Q000254:growth & development / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D012717:Sesquiterpenes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013025:Glycine max / Q000254:growth & development / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development / Q000276:immunology; D053719:Tandem Mass Spectrometry; D000094162:Phytoalexins
chemical_list: D000935:Antifungal Agents; D005960:Glucosides; D007529:Isoflavones; D010636:Phenols; D012717:Sesquiterpenes; D000094162:Phytoalexins
keywords: Colletotrichum gloeosporioides; defence materials; phenolic compounds; soybean sprouts; tandem mass spectrometry