title: Analysis of cytosolic isocitrate dehydrogenase and glutathione reductase 1 in photoperiod-influenced responses to ozone using Arabidopsis knockout mutants.
abstract: Oxidative stress caused by ozone (O3 ) affects plant development, but the roles of specific redox-homeostatic enzymes in O3 responses are still unclear. While growth day length may affect oxidative stress outcomes, the potential influence of day length context on equal-time exposures to O3 is not known. In Arabidopsis Col-0, day length affected the outcome of O3 exposure. In short-days (SD), few lesions were elicited by treatments that caused extensive lesions in long days (LD). Lesion formation was not associated with significant perturbation of glutathione, ascorbate, NADP(H) or NAD(H). To investigate roles of two genes potentially underpinning this redox stability, O3 responses of mutants for cytosolic NADP-isocitrate dehydrogenase (icdh) and glutathione reductase 1 (gr1) were analysed. Loss of ICDH function did not affect O3 -induced lesions, but slightly increased glutathione oxidation, induction of other cytosolic NADPH-producing enzymes and pathogenesis-related gene 1 (PR1). In gr1, O3 -triggered lesions, salicylic acid accumulation, and induction of PR1 were all decreased relative to Col-0 despite enhanced accumulation of glutathione. Thus, even at identical irradiance and equal-time exposures, day length strongly influences phenotypes triggered by oxidants of atmospheric origin, while in addition to its antioxidant function, the GR-glutathione system seems to play novel signalling roles during O3 exposure.
authors.affiliation: UMR1137 EEF, Université de Lorraine, F-54500 Vandoeuvre-lès-Nancy, Cedex, France; UMR1137 EEF, INRA, F-54280, Champenoux, France; IFR110 EFABA, F-54500 Vandoeuvre-lès-Nancy, Cedex, France.
authors.forename: Ata Allah
authors.initials: AA
authors.lastname: Dghim
authors.forename: Amna
authors.initials: A
authors.lastname: Mhamdi
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Vaultier
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Hasenfratz-Sauder
authors.forename: Didier
authors.initials: D
authors.lastname: Le Thiec
authors.forename: Pierre
authors.initials: P
authors.lastname: Dizengremel
authors.forename: Graham
authors.initials: G
authors.lastname: Noctor
authors.forename: Yves
authors.initials: Y
authors.lastname: Jolivet
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology*; D060146:Ecotype; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055786:Gene Knockout Techniques; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000235:genetics* / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010126:Ozone / Q000494:pharmacology*; D010641:Phenotype; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D017440:Photoperiod; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D009243:NAD; D009249:NADP; D010126:Ozone; D007521:Isocitrate Dehydrogenase; C588248:ICDH protein, Arabidopsis; D005980:Glutathione Reductase; D010730:Phosphoenolpyruvate Carboxylase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: NADP-dehydrogenases; glutathione; oxidative stress; ozone; salicylic acid