title: The effect of an NgR1 antagonist on the neuroprotection of cortical axons after cortical infarction in rats.
abstract: We investigated the effect of the soluble Nogo66 receptor (sNgR-Fc) on the protection of cortical axons after cortical infarction in rats. The cortical infarction was induced by photothrombotic cortical injury (PCI) in Sprague-Dawley rats, after which sNgR-Fc was injected into the lateral ventricle. The ipsilesional cortices were harvested for analyses using histochemical and transmission-electron microscope techniques. The involved signaling pathways, which include RhoA, JNK, c-JUN and ATF-2, were detected by Western blot. Serious pathologies were found in the brains of the rats after injury, including edemas in the axoplasms of axons that have no medulla sheath and a thickening or shrinkage in the sheath of the axons that have medulla sheathes. However, these pathologies improved after sNgR-Fc treatment. The levels of GTP-RhoA, p-JNK, p-c-JUN and p-ATF-2 in the PCI group were increased when compared with their levels in the sham-operation group (P < 0.05), and animals receiving the sNgR-Fc treatment showed lower expression levels of these proteins when compared with the sham-operation group (P < 0.05). Our results suggest that sNgR-Fc can alleviate the pathological changes of axons following cortical infarction via decreasing the activation of RhoA/JNK signaling pathways.
authors.affiliation: Department of Emergency, The First Affiliated Hospital, Sun Yat-sen University, 58 ZhongShan II, Guangzhou, 510080, Guangdong, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhan
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Cai
authors.forename: Ying-Qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Hu
authors.forename: Daniel H S
authors.initials: DH
authors.lastname: Lee
authors.forename: Kwok-Fai
authors.initials: KF
authors.lastname: So
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects*; D001812:Blood-Brain Barrier; D002544:Cerebral Infarction / Q000473:pathology / Q000517:prevention & control*; D004789:Enzyme Activation; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors; D046529:Microscopy, Electron, Transmission; D009185:Myelin Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D000070798:Nogo Proteins; D000070816:Nogo Receptor 1; D051381:Rats; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors*; D015398:Signal Transduction
chemical_list: D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D018696:Neuroprotective Agents; D000070798:Nogo Proteins; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; C000603197:Rtn4 protein, rat; C486581:Rtn4r protein, rat