title: Identification of cystathionine γ-synthase and threonine synthase from Cicer arietinum and Lens culinaris.
abstract: In plants, cystathionine γ-synthase (CGS) and threonine synthase (TS) compete for the branch-point metabolite O-phospho-L-homoserine. These enzymes are potential targets for metabolic engineering studies, aiming to alter the flux through the competing methionine and threonine biosynthetic pathways, with the goal of increasing methionine production. Although CGS and TS have been characterized in the model organisms Escherichia coli and Arabidopsis thaliana, little information is available on these enzymes in other, particularly plant, species. The functional CGS and TS coding sequences from the grain legumes Cicer arietinum (chickpea) and Lens culinaris (lentil) identified in this study share approximately 80% amino acid sequence identity with the corresponding sequences from Glycine max. At least 7 active-site residues of grain legume CGS and TS are conserved in the model bacterial enzymes, including the catalytic base. Putative processing sites that remove the targeting sequence and result in functional TS were identified in the target species.
authors.affiliation: Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.
authors.forename: Dominique J K
authors.initials: DJ
authors.lastname: Morneau
authors.forename: Allison F
authors.initials: AF
authors.lastname: Jaworski
authors.forename: Susan M
authors.initials: SM
authors.lastname: Aitken
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D019757:Carbon-Oxygen Lyases / Q000235:genetics* / Q000378:metabolism; D029863:Cicer / Q000201:enzymology / Q000235:genetics*; D017124:Conserved Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D027806:Lens Plant / Q000201:enzymology / Q000235:genetics*; D008715:Methionine / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology / Q000235:genetics; D013912:Threonine / Q000096:biosynthesis; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D013912:Threonine; D008715:Methionine; C022696:O-succinylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase