title: Association between single nucleotide polymorphisms and wheat-dependent exercise-induced anaphylaxis in Chinese population.
abstract: BACKGROUND
  Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a complex disease resulting from interaction of environmental and genetic factors. The aim of this study was to investigate the association of three single nucleotide polymorphisms (SNPs) (IL-4-C590T, IL-4RA A1727G and IL-10-A627C) with WDEIA.
  
  
  METHODS
  SNP genotyping was conducted among the case subset composing 51 patients with WDEIA and four control subsets by sequencing DNA yielded from polymerase chain reaction (PCR). Statistical analysis of genotype/allele's frequencies between cases and controls were carried out through Fisher's exact test with the software of SPSS16.0.
  
  
  RESULTS
  For IL-4-C590T, there were statistically significant differences of genotype frequencies in case-control 1 (P = 0.03) and case-control 4 (P = 0.001) and statistically significant differences of allele frequencies in three case-control models (case-control 1: OR = 4.27 (95%CI = 1.40 - 13.07), P = 0.009; case-control 3: OR = 1.99 (95%CI = 1.13 - 3.50), P = 0.02; case-control 4: OR = 2.39 (95%CI = 1.49 - 3.84), P = 0.001). All other association studies showed no statistically significant (P > 0.05).
  
  
  CONCLUSIONS
  IL-4-C590T may be related to the susceptibility of WDEIA, and the minor allele C might be a potential risk factor accounting for WDEIA. IL-4RA A1727G and IL-10-A627C might not be involved in the occurrence of WDEIA.
authors.affiliation: Department of Allergy, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Cai
authors.forename: Jia
authors.initials: J
authors.lastname: Yin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000707:Anaphylaxis / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D015444:Exercise / Q000502:physiology*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D005903:Gliadin / Q000276:immunology; D005983:Glutens / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014908:Triticum / Q000276:immunology*; D055815:Young Adult
chemical_list: D007073:Immunoglobulin E; D005983:Glutens; D005903:Gliadin