title: Blood plasma surface-enhanced Raman spectroscopy for non-invasive optical detection of cervical cancer.
abstract: Based on blood plasma surface-enhanced Raman spectroscopy (SERS) analysis, a simple and label-free blood test for non-invasive cervical cancer detection is presented in this paper. SERS measurements were performed on blood plasma samples from 60 cervical cancer patients and 50 healthy volunteers. Both the empirical approach and multivariate statistical techniques, including principal component analysis (PCA) and linear discriminant analysis (LDA), were employed to analyze and differentiate the obtained blood plasma SERS spectra. The empirical diagnostic algorithm based on the integration area of the SERS spectral bands (1310-1430 and 1560-1700 cm(-1)) achieved a diagnostic sensitivity of 70% and 83.3%, and a specificity of 76% and 78%, respectively, whereas the diagnostic algorithms based on PCA-LDA yielded a better diagnostic sensitivity of 96.7% and a specificity of 92% for separating cancerous samples from normal samples. This exploratory work demonstrates that a silver nanoparticle based SERS plasma analysis technique in conjunction with PCA-LDA has potential for improving cervical cancer detection and screening.
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Feng
authors.forename: Duo
authors.initials: D
authors.lastname: Lin
authors.forename: Juqiang
authors.initials: J
authors.lastname: Lin
authors.forename: Buhong
authors.initials: B
authors.lastname: Li
authors.forename: Zufang
authors.initials: Z
authors.lastname: Huang
authors.forename: Guannan
authors.initials: G
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Jianji
authors.initials: J
authors.lastname: Pan
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D000465:Algorithms; D001773:Blood Cells / Q000473:pathology*; D016022:Case-Control Studies; D016002:Discriminant Analysis; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D053768:Metal Nanoparticles / Q000737:chemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D061848:Optical Imaging; D010949:Plasma / Q000737:chemistry*; D025341:Principal Component Analysis; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000175:diagnosis*
chemical_list: D012834:Silver