title: Methylation pattern of IFNG in periapical granulomas and radicular cysts.
abstract: BACKGROUND
  Interferon-γ plays an important role in the pathogenesis of periapical lesions, and the methylation of IFNG has been associated with transcriptional inactivation. The purpose of the present study was to investigate IFNG promoter methylation in association with gene transcription and protein levels in periapical granulomas and radicular cysts.
  
  
  METHODS
  Methylation-specific polymerase chain reaction was used to assess the DNA methylation pattern of the IFNG gene in 16 periapical granulomas and 13 radicular cyst samples. The transcription levels of IFNG mRNA were verified by quantitative real-time polymerase chain reaction, and protein expression was evaluated by immunohistochemistry.
  
  
  RESULTS
  All the periapical lesion samples exhibited partial or total methylation of the IFNG gene. In addition, an increased methylation profile was found in radicular cysts compared with periapical granulomas. Increased IFNG mRNA expression was observed in the partially methylated periapical lesion samples relative to the samples that were completely methylated.
  
  
  CONCLUSIONS
  The present study provides the first evidence of the possible impact of IFNG methylation on IFNG transcription in periapical lesions.
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Kelma
authors.initials: K
authors.lastname: Campos
authors.forename: Carolina Cavaliéri
authors.initials: CC
authors.lastname: Gomes
authors.forename: Jeane
authors.initials: J
authors.lastname: de Fátima Correia-Silva
authors.forename: Lucyana Conceição
authors.initials: LC
authors.lastname: Farias
authors.forename: Thiago
authors.initials: T
authors.lastname: Fonseca-Silva
authors.forename: Vanessa Fátima
authors.initials: VF
authors.lastname: Bernardes
authors.forename: Cláudia Maria
authors.initials: CM
authors.lastname: Pereira
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D044127:Epigenesis, Genetic / Q000235:genetics*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010484:Periapical Granuloma / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000502:physiology; D011842:Radicular Cyst / Q000235:genetics*; D018709:Statistics, Nonparametric; D014157:Transcription Factors / Q000235:genetics; D055815:Young Adult
chemical_list: D014157:Transcription Factors; D007371:Interferon-gamma