title: Pesticide tolerant and phosphorus solubilizing Pseudomonas sp. strain SGRAJ09 isolated from pesticides treated Achillea clavennae rhizosphere soil.
abstract: In this study, an attempt was made to identify an effective phosphate solubilizing bacteria from pesticide polluted field soil. Based on the formation of solubilization halo on Pikovskaya's agar, six isolates were selected and screened for pesticide tolerance and phosphate (P) solubilization ability through liquid assay. The results showed that only one strain (SGRAJ09) obtained from Achillea clavennae was found to tolerate maximum level of the pesticides tested and it was phylogenetically identified as Pseudomonas sp. It possessed a wide range of pesticide tolerance, ranging from 117 μg mL(-1) for alphamethrin to 2,600 μg mL(-1) for endosulfan. The available P concentrations increased with the maximum and double the maximum dose of monocrotophos and imidacloprid, respectively. On subjected to FT-IR and HPLC analysis, the presence of organic acids functional group in the culture broth and the production of gluconic acid as dominant acid aiding the P solubilization were identified. On comparison with control broth, monocrotophos and imidacloprid added culture broth showed quantitatively high organic acids production. In addition to gluconic acid production, citric and acetic acids were also observed in the pesticide amended broth. Furthermore, the Pseudomonas sp. strain SGRAJ09 possessed all the plant growth promoting traits tested. In presence of monocrotophos and imidacloprid, its plant growth promoting activities were lower than that of the pesticides unamended treatment.
authors.affiliation: School of Bio Sciences and Technology, Vellore Institute of Technology (VIT), Vellore, 632 014, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Rajasankar
authors.forename: G
authors.initials: G
authors.lastname: Manju Gayathry
authors.forename: A
authors.initials: A
authors.lastname: Sathiavelu
authors.forename: C
authors.initials: C
authors.lastname: Ramalingam
authors.forename: V S
authors.initials: VS
authors.lastname: Saravanan
mesh_terms: D031162:Achillea / Q000382:microbiology*; D000641:Ammonia / Q000378:metabolism; D019755:Carbon-Carbon Lyases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006856:Hydrogen Cyanide / Q000378:metabolism; D007093:Imidazoles / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D008999:Monocrotophos / Q000378:metabolism; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000378:metabolism; D010575:Pesticides / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D058441:Rhizosphere; D017262:Siderophores / Q000378:metabolism; D012988:Soil Microbiology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D007093:Imidazoles; D007210:Indoleacetic Acids; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010575:Pesticides; D010710:Phosphates; D017262:Siderophores; D010758:Phosphorus; D006856:Hydrogen Cyanide; C082359:imidacloprid; D008999:Monocrotophos; C030737:indoleacetic acid; D000641:Ammonia; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases