title: β-Sitosterol and flavonoids isolated from Bauhinia malabarica found during screening for Wnt signaling inhibitory activity.
abstract: Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as β-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC50 values of 0.77, 0.74, and 16.6 μM, respectively.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba, 260-8675, Japan.
authors.forename: Hyun Young
authors.initials: HY
authors.lastname: Park
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Toume
authors.forename: Midori A
authors.initials: MA
authors.lastname: Arai
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyano
authors.forename: Thaworn
authors.initials: T
authors.lastname: Kowithayakorn
authors.forename: Masami
authors.initials: M
authors.lastname: Ishibashi
mesh_terms: D036481:Bauhinia / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017930:Genes, Reporter; D045325:HCT116 Cells; D057809:HEK293 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D044949:Kaempferols / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012997:Solvents / Q000737:chemistry; D014162:Transfection; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D005419:Flavonoids; D044949:Kaempferols; D010936:Plant Extracts; D012855:Sitosterols; D012997:Solvents; C025473:gamma-sitosterol; C021304:hyperoside; D011794:Quercetin; D008156:Luciferases; D000432:Methanol