title: Binding pattern and toxicological effects of lectins from genus Canavalia on bovine sperm.
abstract: The aim of this study was to determine the binding patterns of Canavalia ensiformis (ConA), Canavalia boliviana (ConBol) and Canavalia brasiliensis (ConBr) lectins to bovine sperm and their effects on sperm motility, viability, lipid peroxidation, reactive oxygen species production and fertilization ability. ConA bound to whole spermatozoa, with the exception of the equatorial segment, ConBol did not interact with the acrosome region and ConBr exhibited a fragmented binding pattern. The three lectins decreased sperm motility but did not affect cell viability or lipid peroxidation. Nevertheless, ROS production was increased in comparison to controls and a reduction in the cleavage and blastocyst ratio was induced in comparison to controls. In conclusion, this study determined that structurally similar lectins interact differently with bovine sperm and affect sperm motility, viability, lipid peroxidation, ROS production and fertilization ability in various ways.
authors.affiliation: Laboratório de Embriologia Molecular e Transgênese, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Cristian
authors.initials: C
authors.lastname: Kaefer
authors.forename: Eliza R
authors.initials: ER
authors.lastname: Komninou
authors.forename: Vinicius F
authors.initials: VF
authors.lastname: Campos
authors.forename: Priscila M
authors.initials: PM
authors.lastname: de Leon
authors.forename: Francisco Vassiliepe S
authors.initials: FV
authors.lastname: Arruda
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.forename: Edson H
authors.initials: EH
authors.lastname: Teixeira
authors.forename: Francielli M
authors.initials: FM
authors.lastname: Stefanello
authors.forename: Alethéa G
authors.initials: AG
authors.lastname: Barschak
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Deschamps
authors.forename: Fabiana K
authors.initials: FK
authors.lastname: Seixas
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
mesh_terms: D000818:Animals; D032449:Canavalia; D002417:Cattle; D005260:Female; D005306:Fertilization / Q000187:drug effects; D037102:Lectins / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009865:Oocytes / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D037102:Lectins; D017382:Reactive Oxygen Species