title: Spp1 at the crossroads of H3K4me3 regulation and meiotic recombination.
abstract: In Saccharomyces cerevisiae, all H3K4 methylation is performed by a single Set1 Complex (Set1C) that is composed of the catalytic (Set1) and seven other subunits (Swd1, Swd2, Swd3, Bre2, Sdc1, Spp1 and Shg1). It has been known for quite some time that trimethylated H3K4 (H3K4me3) is enriched in the vicinity of meiotic double-strand breaks (DSBs), but the link between H3K4me3 and the meiotic nuclease Spo11 was uncovered only recently. The PHD-containing subunit Spp1, by interacting with H3K4me3 and Mer2, was shown to promote the recruitment of potential meiotic DSB sites to the chromosomal axis allowing their subsequent cleavage by Spo11. Therefore, Spp1 emerged as a key regulator of the H3K4 trimethylation catalyzed by Set1C and of the formation of meiotic DSBs. These findings illustrate the remarkable multifunctionality of Spp1, which not only regulates the catalytic activity of the enzyme (Set1), but also interacts with the deposited mark, and mediates its biological effect (meiotic DSB formation) independently of the complex. As it was previously described for Swd2, and now for Spp1, we anticipate that other Set1C subunits, in addition to regulating H3K4 methylation, may participate in diverse biological functions inside or outside of the complex.
authors.affiliation: Marseille Cancer Research Center (CRCM);U1068 Inserm; UMR7258 CNRS; Aix-Marseille Univ; Institut Paoli-Calmettes; Marseille, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Acquaviva
authors.affiliation: Marseille Cancer Research Center (CRCM);U1068 Inserm; UMR7258 CNRS; Aix-Marseille Univ; Institut Paoli-Calmettes; Marseille, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Drogat
authors.affiliation: Marseille Cancer Research Center (CRCM);U1068 Inserm; UMR7258 CNRS; Aix-Marseille Univ; Institut Paoli-Calmettes; Marseille, France.
authors.forename: Pierre-Marie
authors.initials: PM
authors.lastname: Dehé
authors.affiliation: Marseille Cancer Research Center (CRCM);U1068 Inserm; UMR7258 CNRS; Aix-Marseille Univ; Institut Paoli-Calmettes; Marseille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: de La Roche Saint-André
authors.affiliation: Marseille Cancer Research Center (CRCM);U1068 Inserm; UMR7258 CNRS; Aix-Marseille Univ; Institut Paoli-Calmettes; Marseille, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Géli
mesh_terms: D000818:Animals; D053903:DNA Breaks, Double-Stranded; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008540:Meiosis; D008745:Methylation; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones; D008239:Lysine; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; C071054:REC107 protein, S cerevisiae; C524367:SPP1 protein, S cerevisiae; C411250:SET1 protein, S cerevisiae; C518938:Spo11 protein, S cerevisiae; D000099257:Meiotic Recombination Protein SPO11
keywords: Histone lysine 4 methylation; Mer2; Set1; Set1C; Spp1; Swd2; meiotic recombination; transcription