title: Cervical uterine lesions: an epidemiological and molecular investigation in midwestern Minas Gerais, Brazil.
abstract: Cervical uterine cancer is the second most frequent form of cancer in the female population and the fourth cause of cancer deaths among Brazilian women. In Divinópolis county, Minas Gerais state, cervical cancer accounted for 6.6% of deaths in 2007. The purpose of this study was to conduct an epidemiological and molecular investigation of samples of pre-neoplastic and neoplastic cervical uterine lesions from patients assisted at public healthcare services in the county. The study was based on clinical-epidemiological descriptions retrieved from cytological examination request forms from 2006 to 2010. For molecular analysis, samples from 95 patients were collected and DNA was extracted using the Chelex 100 method. PCR was performed for detection and typing of HPV 6/11, 16, 18, 31, and 33. Of the 52 168 patients who underwent cytological examination, 625 had pre-neoplastic or neoplastic lesions during the study period. Age distribution was consistent with the literature, with a predominance of patients aged 20-49 years. The microorganism found most frequently was Lactobacillus sp. (65%). Prominent among cellular alterations were cervical intraepithelial neoplasia I (39.7%) and atypical squamous cells of undetermined significance (32%). Molecular analysis revealed 72.6% of positive samples for HPV. HPV 16 (26.3%) was the most frequent type, followed by types 6/11 (7.4%), 18 (5.3%), 33 (2.1%), and 31 (1%). The results provided improved understanding of the association between HPV and cancer in Divinópolis, in addition to providing data that can contribute to the design of measures to prevent and control HPV infection in the county investigated.
authors.affiliation: Federal University of São João del-Rei, Divinópolis, MG 35501-296, Brazil.
authors.forename: Vanessa Regina
authors.initials: VR
authors.lastname: Rocha
authors.forename: Raíssa de Oliveira Aquino
authors.initials: Rde O
authors.lastname: Schüffner
authors.forename: Isabela Iside Gagliardo
authors.initials: II
authors.lastname: Soares
authors.forename: Jacqueline Domingues
authors.initials: JD
authors.lastname: Tibúrcio
authors.forename: Rosy Iara Maciel de Azambuja
authors.initials: RI
authors.lastname: Ribeiro
authors.forename: Débora de Oliveira
authors.initials: Dde O
authors.lastname: Lopes
authors.forename: Luciana Lara
authors.initials: LL
authors.lastname: dos Santos
mesh_terms: D000328:Adult; D001938:Brazil / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000473:pathology* / Q000821:virology; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000473:pathology* / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral