title: Integrated proteomics and metabolomics of Arabidopsis acclimation to gene-dosage dependent perturbation of isopropylmalate dehydrogenases.
abstract: Maintaining metabolic homeostasis is critical for plant growth and development. Here we report proteome and metabolome changes when the metabolic homeostasis is perturbed due to gene-dosage dependent mutation of Arabidopsis isopropylmalate dehydrogenases (IPMDHs). By integrating complementary quantitative proteomics and metabolomics approaches, we discovered that gradual ablation of the oxidative decarboxylation step in leucine biosynthesis caused imbalance of amino acid homeostasis, redox changes and oxidative stress, increased protein synthesis, as well as a decline in photosynthesis, which led to rearrangement of central metabolism and growth retardation. Disruption of IPMDHs involved in aliphatic glucosinolate biosynthesis led to synchronized increase of both upstream and downstream biosynthetic enzymes, and concomitant repression of the degradation pathway, indicating metabolic regulatory mechanisms in controlling glucosinolate biosynthesis.
authors.affiliation: Department of Biology, Genetics Institute, and Plant Molecular & Cellular Biology Program, University of Florida, Gainesville, Florida, United States of America.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dai
authors.forename: Craig P
authors.initials: CP
authors.lastname: Dufresne
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Pang
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D040901:Proteomics / Q000379:methods*
chemical_list: D029681:Arabidopsis Proteins; D008291:Malate Dehydrogenase