title: TAF13 interacts with PRC2 members and is essential for Arabidopsis seed development.
abstract: TBP-Associated Factors (TAFs) are components of complexes like TFIID, TFTC, SAGA/STAGA and SMAT that are important for the activation of transcription, either by establishing the basic transcription machinery or by facilitating histone acetylation. However, in Drosophila embryos several TAFs were shown to be associated with the Polycomb Repressive Complex 1 (PRC1), even though the role of this interaction remains unclear. Here we show that in Arabidopsis TAF13 interacts with MEDEA and SWINGER, both members of a plant variant of Polycomb Repressive Complex 2 (PRC2). PRC2 variants play important roles during the plant life cycle, including seed development. The taf13 mutation causes seed defects, showing embryo arrest at the 8-16 cell stage and over-proliferation of the endosperm in the chalazal region, which is typical for Arabidopsis PRC2 mutants. Our data suggest that TAF13 functions together with PRC2 in transcriptional regulation during seed development.
authors.affiliation: Dipartimento di BioScienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy.
authors.forename: Matias
authors.initials: M
authors.lastname: Lindner
authors.forename: Sara
authors.initials: S
authors.lastname: Simonini
authors.forename: Maarten
authors.initials: M
authors.lastname: Kooiker
authors.forename: Valeria
authors.initials: V
authors.lastname: Gagliardini
authors.forename: Marc
authors.initials: M
authors.lastname: Somssich
authors.forename: Mareike
authors.initials: M
authors.lastname: Hohenstatt
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Simon
authors.forename: Ueli
authors.initials: U
authors.lastname: Grossniklaus
authors.forename: Martin M
authors.initials: MM
authors.lastname: Kater
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D009154:Mutation; D063151:Polycomb Repressive Complex 2; D025941:Protein Interaction Mapping; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C117389:FIS2 protein, Arabidopsis; C111712:MEA protein, Arabidopsis; C574170:PRC2 protein, Arabidopsis; D012097:Repressor Proteins; C513799:SWINGER protein, Arabidopsis; D014157:Transcription Factors; D063151:Polycomb Repressive Complex 2