title: Sanguineous normothermic, intermittent cardioplegia, effects on hypertrophic myocardium. Morphometric, metabolic and ultrastructural studies in rabbits hearts.
abstract: OBJECTIVE
  The present investigation aimed to study the protective effect of intermittent normothermic cardioplegia in rabbit's hypertrophic hearts.
  
  
  METHODS
  The parameters chosen were 1) the ratio heart weight / body weight, 2) the myocardial glycogen levels, 3) ultrastructural changes of light and electron microscopy, and 4) mitochondrial respiration.
  
  
  RESULTS
  1) The experimental model, coarctation of the aorta induced left ventricular hypertrophy; 2) the temporal evolution of the glycogen levels in hypertrophic myocardium demonstrates that there is a significant decrease; 3) It was observed a time-dependent trend of higher oxygen consumption values in the hypertrophic group; 4) there was a significant time-dependent decrease in the respiratory coefficient rate in the hypertrophic group; 5) the stoichiometries values of the ADP: O2 revealed the downward trend of the values of the hypertrophic group; 6) It was possible to observe damaged mitochondria from hypertrophic myocardium emphasizing the large heterogeneity of data.
  
  
  CONCLUSIONS
  The acquisition of biochemical data, especially the increase in speed of glycogen breakdown, when anatomical changes are not detected, represents an important result even when considering all the difficulties inherent in the process of translating experimental results into clinical practice. With regard to the adopted methods, it is clear that morphometric methods are less specific. Otherwise, the biochemical data allow detecting alterations of glycogen concentrations and mitochondria respiration before the morphometric alterations should be detected.
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Clovis
authors.initials: C
authors.lastname: Carbone Junior
authors.forename: José Eduardo de Salles
authors.initials: JE
authors.lastname: Roselino
authors.forename: Valder Rodrigues
authors.initials: VR
authors.lastname: Mello
authors.forename: Paulo Roberto Barbosa
authors.initials: PR
authors.lastname: Evora
authors.forename: Albert Amin
authors.initials: AA
authors.lastname: Sader
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology*; D002312:Cardiomyopathy, Hypertrophic / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D006003:Glycogen / Q000378:metabolism*; D006321:Heart / Q000033:anatomy & histology; D006324:Heart Arrest, Induced / Q000379:methods*; D008929:Mitochondria, Heart / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D009929:Organ Size / Q000502:physiology; D010101:Oxygen Consumption / Q000502:physiology; D011817:Rabbits; D011897:Random Allocation; D018709:Statistics, Nonparametric
chemical_list: D006003:Glycogen