title: Antimicrobial peptides in oyster hemolymph: the bacterial connection.
abstract: We have explored antimicrobial compounds in oyster hemolymph and purified four active peptides with molecular masses of 4464, 3158, 655 and 636 Da. While no exploitable structural elements were obtained for the former three, a partial amino acid sequence (X-P-P-X-X-I-V) was obtained for the latter, named Cg-636. Due to both its low MM and the presence of exotic amino acid residue (X), we suspected a bacterial origin and tracked cultivable hemolymph-resident bacteria of oyster for their antimicrobial abilities. Supernatants of 224 hemolymph resident bacteria coming from 60 oysters were screened against 10 target bacteria including aquaculture pathogens. Around 2% (5 strains) revealed antimicrobial activities. They belong to Pseudoalteromonas and Vibrio genera. Two closely related strains named hCg-6 and hCg-42 have been shown to produce Bacteriocin-Like Inhibitory Substances (BLIS) even in oyster hemolymph. We report herein first BLIS-producing bacteria isolated from bivalve hemolymph. These results strongly suggest that hemolymph resident bacteria may prevent pathogen establishment and pave the way for considering a role of resident bacteria into bivalve defense.
authors.affiliation: Université de Brest, Institut Universitaire de Technologie, Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne EA3882, Université Européenne de Bretagne, Quimper, France.
authors.forename: Diane
authors.initials: D
authors.lastname: Defer
authors.forename: Florie
authors.initials: F
authors.lastname: Desriac
authors.forename: Joël
authors.initials: J
authors.lastname: Henry
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bourgougnon
authors.forename: Michèle
authors.initials: M
authors.lastname: Baudy-Floc'h
authors.forename: Benjamin
authors.initials: B
authors.lastname: Brillet
authors.forename: Patrick
authors.initials: P
authors.lastname: Le Chevalier
authors.forename: Yannick
authors.initials: Y
authors.lastname: Fleury
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D049896:Crassostrea / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D005602:France; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D006458:Hemolymph / Q000382:microbiology; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020539:Sequence Analysis, Protein / Q000662:veterinary
chemical_list: D023181:Antimicrobial Cationic Peptides