title: Spheroid model study comparing the biocompatibility of Biodentine and MTA.
abstract: The primary objective of this study was to assess the biological effects of a new dentine substitute based on Ca₃SiO₅ (Biodentine™) for use in pulp-capping treatment, on pseudo-odontoblastic (MDPC-23) and pulp (Od-21) cells. The secondary objective was to evaluate the effects of Biodentine and mineral trioxide aggregate (MTA) on gene expression in cultured spheroids. We used the acid phosphatase assay to compare the biocompatibility of Biodentine and MTA. Cell differentiation was investigated by RT-qPCR. We investigated the expression of genes involved in odontogenic differentiation (Runx2), matrix secretion (Col1a1, Spp1) and mineralisation (Alp). ANOVA and PLSD tests were used for data analysis. MDPC-23 cells cultured in the presence of MTA had higher levels of viability than those cultured in the presence of Biodentine and control cells on day 7 (P = 0.0065 and P = 0.0126, respectively). For Od-21 cells, proliferation rates on day 7 were significantly lower in the presence of Biodentine or MTA than for control (P < 0.0001). Col1a1 expression levels were slightly lower in cells cultured in the presence of MTA than in those cultured in the presence of Biodentine and in control cells. Biodentine and MTA may modify the proliferation of pulp cell lines. Their effects may fluctuate over time, depending on the cell line considered. The observed similarity between Biodentine and MTA validates the indication for direct pulp-capping claimed by the manufacturers.
authors.affiliation: Faculté d'Odontologie, Univ Rennes1, UEB, Bât. 15, 2 Av du Professeur Léon Bernard, 35043 Rennes Cedex, France. matthieu.perard@univ-rennes1.fr
authors.forename: Matthieu
authors.initials: M
authors.lastname: Pérard
authors.forename: Justine
authors.initials: J
authors.lastname: Le Clerc
authors.forename: Tanguy
authors.initials: T
authors.lastname: Watrin
authors.forename: Fleur
authors.initials: F
authors.lastname: Meary
authors.forename: Fabienne
authors.initials: F
authors.lastname: Pérez
authors.forename: Sylvie
authors.initials: S
authors.lastname: Tricot-Doleux
authors.forename: Pascal
authors.initials: P
authors.lastname: Pellen-Mussi
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects*; D004338:Drug Combinations; D006801:Humans; D009804:Odontoblasts / Q000166:cytology / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D057872:Pulp Capping and Pulpectomy Agents; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C506393:tricalcium silicate