title: Rhizobium pisi sv. trifolii K3.22 harboring nod genes of the Rhizobium leguminosarum sv. trifolii cluster.
abstract: The taxonomic status of the Rhizobium sp. K3.22 clover nodule isolate was studied by multilocus sequence analysis (MLSA) of 16S rRNA and six housekeeping chromosomal genes, as well as by a subsequent phylogenic analysis. The results revealed full congruence with the Rhizobium pisi DSM 30132(T) core genes, thus supporting the same taxonomic position for both strains. However, the K3.22 plasmid symbiosis nod genes demonstrated high sequence similarity to Rhizobium leguminosarum sv. trifolii, whereas the R. pisi DSM 30132(T)nod genes were most similar to R. leguminosarum sv. viciae. The strains differed in the host range nodulation specificity, since strain K3.22 effectively nodulated red and white clover but not vetch, in contrast to R. pisi DSM 30132(T), which effectively nodulated vetch but was not able to nodulate clover. Both strains had the ability to form nodules on pea and bean but they differed in bean cultivar specificity. The R. pisi K3.22 and DSM 30132(T) strains might provide evidence for the transfer of R. leguminosarum sv. trifolii and sv. viciae symbiotic plasmids occurring in natural soil populations.
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marek-Kozaczuk
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Leszcz
authors.forename: Jerzy
authors.initials: J
authors.lastname: Wielbo
authors.forename: Sylwia
authors.initials: S
authors.lastname: Wdowiak-Wróbel
authors.forename: Anna
authors.initials: A
authors.lastname: Skorupska
mesh_terms: D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D058507:Host Specificity; D029902:Medicago / Q000382:microbiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D055170:Plant Root Nodulation; D010957:Plasmids; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016961:Rhizobium leguminosarum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S