title: Th17-relevant cytokines vary with sera of different ANA staining patterns.
abstract: Antinuclear antibodies (ANA) react with components located in the cell nucleus and cytoplasm. Differing ANA staining patterns may reflect the specificity of autoantibodies in sera and indicate some autoimmune diseases specifically, to some extent. Th17-relevant cytokines have been shown to be involved in a variety of autoimmune diseases, but not consistently. In this study, we investigated whether differences in Th17-relevant cytokines exist between different ANA pattern sera. Sera of 64 ANA-positive patients (12 homogeneous, 13 speckled particle, 11 nucleolar, 15 centromere, 6 peripheral nuclear) and 16 healthy donors were analyzed for IL-17, IL-6, IL-21, IL-22, IL-23 (p19), and TGF-β, and subsequently correlations between IL-17 and IL-6, IL-21, IL-22, IL-23, and TGF-β were analyzed. Results showed that these Th17-relevant cytokines varied with different ANA-positive sera compared with healthy donors, except TGF-β. Among them, IL-21 and IL-22 were higher with all ANA-positive sera and IL-17, IL-6, and IL-23 were higher with three or more ANA staining sera. No significant difference in these cytokines was seen between the different ANA staining sera except IL-17 levels in sera of peripheral nuclear staining positive subjects were higher than nucleolar. Additionally, in ANA-positive sera, IL-17 correlated with IL-6, IL-21, IL-22, and IL-23, but not with TGF-β. Thus, we demonstrated that Th17-relevant cytokines varied with different ANA staining pattern sera, suggesting that Th17-relevant cytokines play differing roles in autoimmune diseases.
authors.affiliation: Department of Laboratory Medicine, Gongli Hospital, Second Military Medicine University, Pudong New Area, Shanghai, 200135, China. hjh7677@sina.com
authors.forename: Jinhui
authors.initials: J
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
authors.forename: Denghai
authors.initials: D
authors.lastname: Zhang
authors.forename: Chaolin
authors.initials: C
authors.lastname: Qiu
authors.forename: Ling
authors.initials: L
authors.lastname: Hua
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Xie
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: He
authors.forename: Hongxing
authors.initials: H
authors.lastname: Ye
mesh_terms: D000974:Antibodies, Antinuclear / Q000097:blood* / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D016207:Cytokines