title: MiR-21 down-regulation suppresses cell growth, invasion and induces cell apoptosis by targeting FASL, TIMP3, and RECK genes in esophageal carcinoma.
abstract: BACKGROUND
  miR-21 is overexpressed in esophageal squamous cell carcinoma (ESCC) and is thought to be correlated with the development of the cancer. The target gene of miR-21 including FASL, TIMP3 and RECK is revealed by researchers. miR-21 may be involved in the tumorgenesis of ESCC by targeting FASL, TIMP3 and RECK.
  
  
  OBJECTIVE
  The purpose of this study was to explore the mechanism of miR-21 in the development of ESCC.
  
  
  METHODS
  miR-21 expression in ESCC and the matched non-malignant adjacent tissues (NMATs) was examined by qRT-PCR. Cell growth, cell apoptosis and cell invasion ability of EC9706 and EC-1 cells was examined after the cells were transfected with miR-21 inhibitor. The potential target genes of miR-21 including FASL, TIMP3 and RECK were examined by western blot and Luciferase reporter assay.
  
  
  RESULTS
  miR-21 expression was increased significantly in ESCC tissues compared with NMAT. miR-21 down-regulation inhibits cell growth, cell invasion and induces cells to apoptosis. FASL, TIMP3 and RECK are direct targets of miR-21.
  
  
  CONCLUSIONS
  miR-21 down-regulation inhibits cell growth, invasion and induces cells to apoptosis by targeting FASL, TIMP3 and RECK genes.
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. wnsky@zzu.edu.cn
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Chao-Qi
authors.initials: CQ
authors.lastname: Zhang
authors.forename: Jia-Huan
authors.initials: JH
authors.lastname: He
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Duan
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Ji
authors.forename: Wen-Qiao
authors.initials: WQ
authors.lastname: Zang
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yun-Yun
authors.initials: YY
authors.lastname: Ma
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D053222:Fas Ligand Protein / Q000378:metabolism*; D005260:Female; D058851:GPI-Linked Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D058851:GPI-Linked Proteins; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C115460:RECK protein, human; C088390:TIMP3 protein, human; D019717:Tissue Inhibitor of Metalloproteinase-3