title: Corrective role of Eugenia jambolana on testicular impairment in streptozotocin-induced diabetic male albino rat: an approach through genomic and proteomic study.
abstract: The present study was conducted to explore the effect of ethyl acetate fraction of hydro-methanolic (40 : 60) extract of seed of Eugenia jambolana on testicular impairment in diabetic rats. In this respect, biomarkers of oxidative stress, genomics and proteomics in testicular tissue were assessed. Side by side, glycated haemoglobin, serum testosterone, activities of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase in serum, epididymal sperm count including reproductive organosomatic indices were evaluated. Results indicate that a significant recovery (P < 0.05) in the levels of these parameters in fraction-treated diabetic group in comparison with diabetic control. A significant recovery was noted (P < 0.05) in the expression of Bax and Bcl-2 gene towards the control after the treatment of said fraction. Histological study also focused a significant recovery (P < 0.05) in the number of different generation of germ cells at stage VII of spermatogenesis in fraction-treated diabetic group. The said fraction treatment to diabetic rat can recover the activities of serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase significantly towards the control (P < 0.05). Finally, it may be concluded that ethyl acetate fraction of seed of E. jambolana has a promiseable remedial effect on diabetes-induced testicular dysfunctions in male rat without inducing any metabolic toxicity.
authors.affiliation: Department of Bio-Medical Laboratory Science and Management (UGC Innovative Programme Funded Department under Xth-5 Year Plan), Vidyasagar University, Midnapore, West Bengal, India.
authors.forename: A
authors.initials: A
authors.lastname: Ghosh
authors.forename: K
authors.initials: K
authors.lastname: Jana
authors.forename: K M
authors.initials: KM
authors.lastname: Ali
authors.forename: D
authors.initials: D
authors.lastname: De
authors.forename: K
authors.initials: K
authors.lastname: Chatterjee
authors.forename: D
authors.initials: D
authors.lastname: Ghosh
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology*; D005632:Fructose / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D019254:Genes, bcl-2; D023281:Genomics; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D040901:Proteomics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012669:Seminal Vesicles / Q000187:drug effects / Q000378:metabolism; D012670:Seminiferous Epithelium / Q000187:drug effects / Q000473:pathology; D013076:Sperm Count; D027842:Syzygium; D013737:Testis / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D013739:Testosterone / Q000097:blood; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D015415:Biomarkers; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D005632:Fructose; D013739:Testosterone; D006913:Hydroxysteroid Dehydrogenases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Apoptosis; Eugenia jambolana; oxidative stress; streptozotocin; testicular dysfunction