title: Simultaneous angular multiplexing optical projection tomography at shifted focal planes.
abstract: We describe an angular multiplexing technique for optical projection tomography that improves resolution, signal-to-noise ratio, and imaging speed by ameliorating the trade-off between spatial resolution and depth of field and improving the light collection efficiency. Here we demonstrate that imaging at two orthogonal angular projections simultaneously, focused on shifted planes in the sample, improves the average spatial resolution by ~20% and the light collection efficiency by a factor of ~4, thereby enabling increased acquisition speed and reduced light dose.
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, London, UK. l.chen10@imperial.ac.uk
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.forename: Natalie
authors.initials: N
authors.lastname: Andrews
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Paul
authors.initials: P
authors.lastname: Frankel
authors.forename: James
authors.initials: J
authors.lastname: McGinty
authors.forename: Paul M W
authors.initials: PM
authors.lastname: French
mesh_terms: D000818:Animals; D007091:Image Processing, Computer-Assisted; D013623:Tail; D013997:Time Factors; D041622:Tomography, Optical / Q000379:methods*; D015027:Zebrafish