title: Lentiviral delivery of biglycan promotes proliferation and increases osteogenic potential of bone marrow-derived mesenchymal stem cells in vitro.
abstract: Mesenchymal stem cells (MSCs) are multipotent progenitor nonhematopoietic cells that have emerged as an attractive cell source for tissue engineering. Biglycan (BGN), an extracellular matrix component, has been reported to play a crucial role in bone formation. However, the role of BGN in MSCs remains unknown. Using lentiviral gene delivery, we sought to investigate the cellular effects of BGN on proliferation and osteogenic potential of bone marrow-derived MSCs in vitro. We found that MSCs with lentiviral transduction of BGN exhibited an increase in growth ability and the percentage of the S phase, together with significantly reduced mRNA levels of p21 and p27. Furthermore, lentiviral transduction of BGN in MSCs also increased alkaline phosphatase activity, stimulated the mineralization capacity, and enhanced expression of Runx2, Osteocalcin, collagen I, transforming growth factor (TGF)-β1, p-Smad2 and p-Smad3. Taken together, our data suggest that BGN overexpression positively regulates proliferation and osteogenic potential of MSCs, and TGF-β signaling pathway may be involved in BGN-induced osteogenic potential of MSCs.
authors.affiliation: Department of Orthopaedics, The Orthopaedic Hospital of Shenyang City, 115 Dongbei Road, Shenyang, 110044, Liaoning Province, People's Republic of China. drwubo@live.com
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
authors.forename: Damu
authors.initials: D
authors.lastname: Zhu
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Sun
authors.forename: Suyuan
authors.initials: S
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Xue
authors.forename: Mingchang
authors.initials: M
authors.lastname: Du
authors.forename: Ximeng
authors.initials: X
authors.lastname: Yin
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D058578:Biglycan / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000201:enzymology; D049109:Cell Proliferation; D002469:Cell Separation; D003094:Collagen / Q000378:metabolism; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016086:Lentivirus / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism; D014161:Transduction, Genetic; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D058578:Biglycan; D051785:Smad Proteins; D016212:Transforming Growth Factor beta; D003094:Collagen; D000469:Alkaline Phosphatase