title: [Expression of 5-lipoxygenase in hippocampal CA1 neuronal damage following global cerebral ischemia in rats].
abstract: To determine 5-lipoxygenase (5-LOX) expression and the effect of zileuton, a selective 5-LOX inhibitor,on hippocampal neuron injury induced by global cerebral ischemia in rats.
  
  
  
  Global cerebral ischemia was induced by bilateral common carotid artery occlusion combined with hypotension in rats. 5-LOX expression was detected by Western blot analyses and 5-LOX localization was visualized by immunohistochemistry and double immunofluorescence methods. The 5-LOX inhibitor zileuton (10, 30, 50 mg/kg) was orally administered for 3 d after ischemia.
  
  
  
  The 5-LOX expression was increased in the ischemic hippocampus on d1-7 (peaked at d3), and 5-LOX protein was primarily localized in neurons and translocated to the nuclei in the hippocampal CA1 region after ischemia. The 5-LOX inhibitor zileuton (30, 50 mg/kg) reduced ischemia-induced hippocampal neurons death 3d after ischemia.
  
  
  
  5-LOX is involved in global cerebral ischemic damage in rats, and the 5-LOX inhibitor zileuton has a protective effect on neuronal damage in the rat hippocampus following global cerebral ischemia.
authors.affiliation: Department of Pharmacology, Hangzhou Normal University, Hangzhou, China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Chen
authors.forename: Chengtan
authors.initials: C
authors.lastname: Li
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Huayang
authors.initials: H
authors.lastname: Han
authors.forename: Erqing
authors.initials: E
authors.lastname: Wei
authors.forename: Lihui
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D056547:CA1 Region, Hippocampal / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D006918:Hydroxyurea / Q000031:analogs & derivatives / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016859:Lipoxygenase Inhibitors; D001094:Arachidonate 5-Lipoxygenase; C063449:zileuton; D006918:Hydroxyurea