title: In vitro approaches to evaluate toxicity induced by organotin compounds tributyltin (TBT), dibutyltin (DBT), and monobutyltin (MBT) in neuroblastoma cells.
abstract: The toxic effects of the organotin compounds (OTCs) monobutyltin (MBT), dibutyltin (DBT), and tributyltin (TBT) were evaluated in vitro in a neuroblastoma human cell line. Mechanisms of cell death, apoptosis versus necrosis, were studied by using several markers: inhibition of cell viability and proliferation, F-actin, and mitochondrial membrane potential changes as well as reactive oxygen species (ROS) production and DNA fragmentation. The most toxic effects were detected with DBT and TBT even at very low concentrations (0.1-1 μM). In contrast, MBT induced lighter cytotoxic changes at the higher doses tested. None of the studied compounds stimulated propidium iodide uptake, although the most toxic chemical, TBT, caused lactate dehydrogenase release at the higher concentrations tested. These findings suggest that in neuroblastoma, OTC-induced cytotoxicity involves different pathways depending on the compound, concentration, and incubation time. A screening method for DBT and TBT quantification based on cell viability loss was developed, allowing a fast detection alternative to complex methodology.
authors.affiliation: Food Safety Division, ANFACO-CECOPESCA, Campus Univ. 16, 36310 Vigo PO, Spain.
authors.forename: Martiña
authors.initials: M
authors.lastname: Ferreira
authors.forename: Lucía
authors.initials: L
authors.lastname: Blanco
authors.forename: Alejandro
authors.initials: A
authors.lastname: Garrido
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vieites
authors.forename: Ana G
authors.initials: AG
authors.lastname: Cabado
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009447:Neuroblastoma / Q000378:metabolism; D009498:Neurotoxins / Q000633:toxicity; D009947:Organotin Compounds / Q000633:toxicity*; D017382:Reactive Oxygen Species; D018675:Toxicity Tests / Q000379:methods*; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D009498:Neurotoxins; D009947:Organotin Compounds; D017382:Reactive Oxygen Species; D014220:Trialkyltin Compounds; C516409:mono-n-butyltin; C056189:di-n-butyltin; C011559:tributyltin; D007770:L-Lactate Dehydrogenase