title: The circadian binding of CLOCK protein to the promoter of C/ebpα gene in mouse cells.
abstract: C/EBPα plays important roles in metabolism as well as in the maintenance of energy homeostasis. Here we describe loss of the circadian oscillation of C/ebpα expression in liver of Clock mutant mice. Reporter assays indicate Clock and Bmal significantly induced C/ebpα gene expression whereas Cry suppressed. Real time reporter assays showed that two mutated E-boxes disrupted C/ebpα promoter dependent-oscillation. Chromatin immunoprecipitation suggests Clock can bind to two E-boxes in the C/ebpα promoter with a circadian manner in vivo. Thus, C/ebpα gene transcription is under circadian control of a core clock component, Clock. The data suggests that circadian disturbances may affect metabolic abnormalities through the C/ebpα pathway in liver.
authors.affiliation: Ishida Group of Clock Gene, National Institute of Advanced Science and Technology (AIST) 6-5 Central, Tsukuba, Ibaraki, Japan.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Doi
authors.forename: Kumpei
authors.initials: K
authors.lastname: Ito
authors.forename: Masami
authors.initials: M
authors.lastname: Shimoda
authors.forename: Norio
authors.initials: N
authors.lastname: Ishida
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics*; D056926:CLOCK Proteins / Q000235:genetics / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology*; D024721:E-Box Elements; D015870:Gene Expression; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D012333:RNA, Messenger; D056926:CLOCK Proteins