title: Efficacy of early treatment on 52 patients with preneoplastic hepatitis B virus-associated hepatocellular carcinoma by compound Phyllanthus Urinaria L.
abstract: OBJECTIVE
  To observe the change in the number of antibodies of preneoplastic hepatocellular carcinoma (HCC) using early treatment by Compound Phyllanthus Urinaria L. (CPUL) on patients with preneoplastic hepatitis B virus (HBV)-associated HCC.
  
  
  METHODS
  A total of 102 cirrhosis patients with regenerative or dysplastic nodules whose sera were tested positive for at least one of these six proteins (five up-regulated genes URG4, URG7, URG11, URG12 and URG19, and one down-regulated gene DRG2) were assigned randomly to two groups using continual random codes by SPSS software. Fifty-two patients were in the treatment group and 50 patients were in the control group. CPUL was used in the treatment group for 3 years, while the control group did not receive any treatment. The changes in HBV-DNA level, number of antibodies, and hepatocarcinogenesis occurred were observed. Patients who did not develop HCC were followed up for another 2 years.
  
  
  RESULTS
  HBV-DNA levels decreased ⩾2log in 22.2% (10/45) of patients in the treatment group in contrast to only 5.0% (2/40) of patients in the control group (P=0.0228). The number of antibodies that were tested positive in the treatment group (1.08±1.01) was significantly lower compared with the control group (2.11±1.12) after 24 months of drug treatment (P<0.01). Both the positive rates of anti-URG11 (33/52) and anti-URG19 (31/52) were over 60% at baseline in the two groups, and were decreased to 48.1% (25/52) and 46.2% (24/52) respectively at 36 months of drug treatment, while the rates increased to 68.0% (34/50) and 66.0% (33/50) respectively (P=0.0417, P=0.0436) in the control group. The positive rate of anti-DRG2 was increased to 55.8% (29/52) at 36 months of drug treatment, while in the control group was decreased to 36.0% (18/50, P=0.0452). Among the 102 patients who developed HCC, 2 were in the treatment group and 9 were in the control group, meaning that a significant difference between the two groups (P=0.0212). In 11 patients who developed HCC, anti-URG11 and anti-URG19 were always positive, while anti-DRG2 was negative. Patients newly developing HCC were 6 (20.0%) in the control group, and only one (2.5%) in the treatment group (P=0.0441) during 2-year follow-up after the end of the treatment.
  
  
  CONCLUSIONS
  Anti-URG11, anti-URG19 and anti-DRG2 could be used as early markers in the prediction of the therapeutic efficacy of CPUL in treating preneoplastic HCC. CPUL is useful in preventing or delaying the development of HBV-associated cirrhosis to HCC.
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China. tgd755@163.com.
authors.forename: Guang-dong
authors.initials: GD
authors.lastname: Tong
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Da-qiao
authors.initials: DQ
authors.lastname: Zhou
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Chun-shan
authors.initials: CS
authors.lastname: Wei
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Jin-song
authors.initials: JS
authors.lastname: He
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Chun-ling
authors.initials: CL
authors.lastname: Xiao
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Xin-liang
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Ying-jun
authors.initials: YJ
authors.lastname: Zheng
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Si-nuan
authors.initials: SN
authors.lastname: Chen
authors.affiliation: Department of Liver Disease, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, 518033, China.
authors.forename: Hai-hong
authors.initials: HH
authors.lastname: Tang
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000628:therapy* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D008113:Liver Neoplasms / Q000628:therapy* / Q000821:virology; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D011230:Precancerous Conditions / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D010936:Plant Extracts
keywords: carcinoma; compound phyllanthus urinaria L.; hepatic cell; serologic preneoplastic markers of hepatocellular carcinoma