title: Visualization of mouse nodal cilia and nodal flow.
abstract: The earliest left-right asymmetry in mouse development arises in 7.5 days, at the ventral surface of the embryonic node. The node cells possess monocilia beating in rotatory fashion to generate fluid flow from the right to the left (nodal flow). The direction of nodal flow will determine the side of expression of nodal, the responsible gene for "leftness." Nodal flow is visualized by combination of DIC (differential interference contrast) and microbeads in culture medium. Node cilia movement is visualized by DIC, a high-speed camera, and image processing.
authors.affiliation: National Institute for Basic Biology, Laboratory for Spatiotemporal Regulations, Okazaki, Japan. snonaka@nibb.ac.jp
authors.forename: Shigenori
authors.initials: S
authors.lastname: Nonaka
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002923:Cilia / Q000502:physiology*; D004622:Embryo, Mammalian / Q000378:metabolism / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D051379:Mice; D008853:Microscopy