title: Accelerated mice skin acute wound healing in vivo by combined treatment of argon and helium plasma needle.
abstract: OBJECTIVE
  The efficacy of a direct application of plasma needle to in vivo wound healing was experimentally studied in mice. This kind of plasma has achieved considerable success in blood coagulation and tissue restoration in mice. In the development of the present study, an argon plasma needle was chosen for coagulation purposes, whereas for healing purposes, a helium plasma needle was used.
  
  
  METHODS
  Treatment was applied with a plasma needle produced by argon and helium to a wound induced in laboratory mice. Tissue regeneration was carried out by three argon plasma treatments with 0.5 SLPM flow for 1 min and three treatments of helium with 1.5 SLPM flow. Intervals between each treatment were 5 min and 60 min for argon and helium plasmas, respectively, thus completing a total treatment time of 180 min. Histological sections were performed to corroborate the internal bleeding and tissue regeneration.
  
  
  RESULTS
  After three treatments with argon plasma, the blood produced in the wound was coagulated and protein material appeared. By means of treatment with helium plasma, an approach of the wound edges was produced until the conclusion thereof. These results were corroborated histologically.
  
  
  CONCLUSIONS
  This type of acceleration during the skin wound healing process can be attributed to the formation of reactive species such as NO, which were increased in the helium plasma needle with respect to the argon plasma needle.
authors.affiliation: Plasma Physics Laboratory and Cell Radiobiology Laboratory, Instituto Nacional de Investigaciones Nucleares, México D.F., Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: García-Alcantara
authors.forename: Régulo
authors.initials: R
authors.lastname: López-Callejas
authors.forename: Pedro R
authors.initials: PR
authors.lastname: Morales-Ramírez
authors.forename: Rosendo
authors.initials: R
authors.lastname: Peña-Eguiluz
authors.forename: Raúl
authors.initials: R
authors.lastname: Fajardo-Muñoz
authors.forename: Antonio
authors.initials: A
authors.lastname: Mercado-Cabrera
authors.forename: Samuel R
authors.initials: SR
authors.lastname: Barocio
authors.forename: Raúl
authors.initials: R
authors.lastname: Valencia-Alvarado
authors.forename: Benjamín G
authors.initials: BG
authors.lastname: Rodríguez-Méndez
authors.forename: Arturo E
authors.initials: AE
authors.lastname: Muñoz-Castro
authors.forename: Anibal
authors.initials: A
authors.lastname: de la Piedad-Beneitez
authors.forename: Israel A
authors.initials: IA
authors.lastname: Rojas-Olmedo
mesh_terms: D000208:Acute Disease / Q000628:therapy; D000818:Animals; D001128:Argon / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006371:Helium / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D009339:Needles; D009569:Nitric Oxide / Q000378:metabolism; D058626:Plasma Gases / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D012867:Skin / Q000187:drug effects*; D012871:Skin Diseases / Q000628:therapy; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D058626:Plasma Gases; D006371:Helium; D009569:Nitric Oxide; D001128:Argon