title: Multivariate determination of 13CO2/12CO2 ratios in exhaled mouse breath with mid-infrared hollow waveguide gas sensors.
abstract: The (12)CO2/(13)CO2 isotope ratio is a well-known marker in breath for a variety of biochemical processes and enables monitoring, e.g., of the glucose metabolism during sepsis. Using animal models-here, at a mouse intensive care unit-the simultaneous determination of (12)CO2 and (13)CO2 within small volumes of mouse breath was enabled by coupling a novel low-volume hollow waveguide gas cell to a compact Fourier transform infrared spectrometer combined with multivariate data evaluation based on partial least squares regression along with optimized data preprocessing routines.
authors.affiliation: Institute of Analytical and Bioanalytical Chemistry, University of Ulm, Ulm, Germany.
authors.forename: Felicia
authors.initials: F
authors.lastname: Seichter
authors.forename: Andreas
authors.initials: A
authors.lastname: Wilk
authors.forename: Katharina
authors.initials: K
authors.lastname: Wörle
authors.forename: Seong-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Josef A
authors.initials: JA
authors.lastname: Vogt
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wachter
authors.forename: Peter
authors.initials: P
authors.lastname: Radermacher
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
mesh_terms: D000818:Animals; D001944:Breath Tests / Q000295:instrumentation*; D002245:Carbon Dioxide / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis; D003627:Data Interpretation, Statistical; D004867:Equipment Design; D019544:Equipment Failure Analysis; D045853:Exhalation / Q000502:physiology*; D051379:Mice; D015999:Multivariate Analysis; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014159:Transducers
chemical_list: D002247:Carbon Isotopes; D002245:Carbon Dioxide