title: Humanin binds MPP8: mapping interaction sites of the peptide and protein.
abstract: Humanin (HN), a 24-amino acid peptide encoded by the mitochondrial 16S rRNA gene, was discovered by screening a cDNA library from the occipital cortex of a patient with Alzheimer's disease (AD) for a protection factor against AD-relevant insults. Earlier, using the yeast two-hybrid system, we have identified the M-phase phosphoprotein 8 (MPP8) as a binding partner for HN. In the present work, we further confirmed interaction of HN with MPP8 in co-immunoprecipitation experiments and localized an MPP8-binding site in the region between 5 and 12 aa. of HN. We have also shown that an MPP8 fragment (residues 431-560) is sufficient to bind HN. Further studies on functional consequences of the interaction between the potential oncopetide and the oncoprotein may elucidate some aspects of the molecular mechanisms of carcinogenesis.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Maximov
authors.forename: Alina V
authors.initials: AV
authors.lastname: Martynenko
authors.forename: Inga P
authors.initials: IP
authors.lastname: Arman
authors.forename: Vyacheslav Z
authors.initials: VZ
authors.lastname: Tarantul
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D015723:Gene Library; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D060066:Protein Interaction Maps
chemical_list: D000596:Amino Acids; D047908:Intracellular Signaling Peptides and Proteins; C547278:MPHOSPH8 protein, human; D015513:Oncogene Proteins; D010750:Phosphoproteins; C427211:humanin