title: The addressing fragment of mitogaligin: first insights into functional and structural properties.
abstract: Mitogaligin is a mitochondrion-targeting protein involved in cell death. The sequence of the protein is unrelated to that of any known pro- or antiapoptotic protein. Mitochondrial targeting is controlled by an internal sequence from residues 31 to 53, and although this sequence is essential and sufficient to provoke cell death, the precise mechanism of action at the mitochondrial membrane remains to be elucidated. Here, by focusing on the [31-53] fragment, we first assessed and confirmed its cell cytotoxicity by microinjection. Subsequently, with the aid of membrane models, we evaluated the impact of the membrane environment on the 3D structure of the peptide and on how the peptide is embedded and oriented within membranes. The fragment is well organized, even though it does not contain a canonical secondary structure, and adopts an interfacial location. Structural comparison with other membrane-interacting Trp-rich peptides demonstrated similarities with the antimicrobial peptide tritrpcidin.
authors.affiliation: Centre de Biophysique Moléculaire, CNRS UPR4301 affiliated to the University of Orléans, Rue Charles Sadron, 45071 Orléans cedex 2, France.
authors.forename: Violette
authors.initials: V
authors.lastname: Senille
authors.forename: Dominique
authors.initials: D
authors.lastname: Lelievre
authors.forename: Françoise
authors.initials: F
authors.lastname: Paquet
authors.forename: Norbert
authors.initials: N
authors.lastname: Garnier
authors.forename: Ned
authors.initials: N
authors.lastname: Lamb
authors.forename: Alain
authors.initials: A
authors.lastname: Legrand
authors.forename: Agnès F
authors.initials: AF
authors.lastname: Delmas
authors.forename: Céline
authors.initials: C
authors.lastname: Landon
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002478:Cells, Cultured; D003603:Cytotoxins / Q000737:chemistry / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D037161:Galectins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D001798:Blood Proteins; D003603:Cytotoxins; D037161:Galectins; C428445:LGALS3 protein, human