title: EL4 cell-based colorimetric toxin neutralization activity assays for determination of neutralizing anti-ricin antibodies.
abstract: A recombinant ricin toxin A-chain 1-33/44-198 vaccine (RVEc), developed at the United States Army Medical Research Institute of Infectious Diseases as a vaccine candidate, is under investigation in a phase 1 clinical study. To effectively evaluate the immunogenicity of this ricin vaccine and to eliminate the use of radioactive material, an EL4 cell-based colorimetric toxin neutralization activity (TNA) assay using a CellTiter 96 AQueous One Solution Cell Proliferation Assay Reagent has been developed, optimized, and applied in the vaccine efficacy studies. The TNA assay measures the protective neutralizing anti-ricin antibodies in animal sera by determining the cell viability after ricin exposure in the assay system and comparing it to a purified mouse polyclonal antiricin IgG standard curve. The standard curve of the anti-ricin TNA assay closely fits a four-parameter logistic regression model. The unknown test sample concentration was expressed as microg/mL, but not the 50% effective concentration (EC50), which was determined by most TNA assays. The neutralizing endpoint titers, not the 50% effective dilution (ED50), of human specimens were measured with the TNA assay in support of the clinical study of the RVEc vaccine. The optimal amount of ricin toxin, EL4 cells, and concentration of standards used in the assay system was established to minimize false-negative and false-positive results of serum specimens from the nonclinical and clinical studies of RVEc. The testing conditions were adjusted to optimize assay performance. The colorimetric TNA assay replaced a radioactive TNA assay previously used in the ricin vaccine studies.
authors.affiliation: United States Army Medical Research Institute for Infectious Diseases, Fort Detrick, MD 21702, USA. changhong.lindsey2@us.army.mil
authors.forename: Changhong Y
authors.initials: CY
authors.lastname: Lindsey
authors.forename: J Edward
authors.initials: JE
authors.lastname: Brown
authors.forename: Nahid R
authors.initials: NR
authors.lastname: Torabazar
authors.forename: Leonard A
authors.initials: LA
authors.lastname: Smith
mesh_terms: D057134:Antibodies, Neutralizing / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D009500:Neutralization Tests; D012276:Ricin / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D012276:Ricin