title: Reduced beta-catenin expression in the hippocampal CA1 region following transient cerebral ischemia in the gerbil.
abstract: Beta-catenin, a transcription factor, plays a critical role in cell survival and degradation after stroke. In this study, we examined changes of expression in beta-catenin in the hippocampal CA1 region of the gerbil following 5 min of transient cerebral ischemia. We observed neuronal damage using cresyl violet staining, neuronal nuclei immunohistochemistry and Fluro-Jade B immunofluorescence. Four days after ischemia-reperfusion (I-R), most of pyramidal cells in the CA1 region were damaged. In addition, early damage in dendrites was detected 1 day after I-R by immunohistochemical staining for microtubule-associated protein 2 (MAP-2), and MAP-2 immunoreactivity was hardly detected in the CA1 region 4 days after I-R. We found that beta-catenin (a synapse-enriched cell adhesion molecule) was well expressed in dendrites before I-R. Its immunoreactivity was well colocalized with MAP-2. Chronological change of beta-catenin immunoreactivity was novelty in the present study. Twelve hours after I-R, its immunoreactivity was decreased in the stratum radiatum of the CA1 region, however, its immunoreactivity was increased 1 and 2 days after I-R, and decreased sharply 4 days after I-R. However, we did not find any change in beta-catenin immunoreactivity in the CA2 and CA3 region. In brief, we suggest that early change of beta-catenin expression in the stratum pyramidale of ischemic hippocampal CA1 region is associated with early dendrite damage following transient cerebral ischemia.
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Jeong-Hwi
authors.initials: JH
authors.lastname: Cho
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: In Hye
authors.initials: IH
authors.lastname: Kim
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Young-Myeong
authors.initials: YM
authors.lastname: Kim
authors.forename: Bonghee
authors.initials: B
authors.lastname: Lee
authors.forename: Jun Hwi
authors.initials: JH
authors.lastname: Cho
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism*; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051176:beta Catenin