title: Expression of plasminogen activator inhibitor-2 is negatively associated with invasive potential in hepatocellular carcinoma cells.
abstract: OBJECTIVE
  To investigate the association between plasminogen activator inhibitor (PAI)-2 expression and invasive potential in hepatocellular carcinoma (HCC) cells.
  
  
  METHODS
  The HCC cell lines with high, low, and non-metastatic potentials, namely MHCC97-H, MHCC97-L, and SMMC-7721 respectively, were cultured in vitro. Matrigel invasion assay and Western blot of PAI-2 protein expression were conducted.
  
  
  RESULTS
  The number of invaded cells in MHCC97-L was significantly higher than that in SMMC-7721 (P=0.005), whereas that in MHCC97-H was higher than in MHCC97-L (P=0.017) and SMMC-7721 (P=0.001). Contrarily, PAI-2 protein expression was gradually reducing from SMMC-7721, MHCC97-L, to MHCC97-H (MHCC97-H vs. MHCC97-L, P<0.001; MHCC97-H vs. SMMC-7721, P=0.001; MHCC97-L vs. SMMC-7721, P=0.001). The Pearson's correlation analysis revealed a significant negative association between invaded cell number and PAI-2 expression (r=-0.892, P=0.001).
  
  
  CONCLUSIONS
  PAI-2 expression may be negatively associated with the invasive potential of HCC.
authors.affiliation: Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jin
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Ke-min
authors.initials: KM
authors.lastname: Jin
authors.forename: Bao-cai
authors.initials: BC
authors.lastname: Xing
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D017396:Plasminogen Activator Inhibitor 2 / Q000502:physiology*
chemical_list: D017396:Plasminogen Activator Inhibitor 2