title: Nucleic acid sensing by an orthogonal reporter system based on homo-DNA.
abstract: We have developed an assay for single strand DNA or RNA detection which is based on the homo-DNA templated Staudinger reduction of the profluorophore rhodamine-azide. The assay is based on a three component system, consisting of a homo-DNA/DNA hybrid probe, a set of homo-DNA reporter strands and the target DNA or RNA. We present two different formats of the assay (Omega probe and linear probe) in which the linear probe was found to perform best with catalytic turnover of the reporter strands (TON: 8) and a match/mismatch discrimination of up to 19. The advantage of this system is that the reporting (homo-DNA) and sensing (DNA) domain are decoupled from each other since the two pairing systems are bioorthogonal. This allows independent optimization of either domain which may lead to higher selectivity in in vivo imaging.
authors.affiliation: University of Bern, Department of Chemistry and Biochemistry, Bern, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Stoop
authors.forename: Camille
authors.initials: C
authors.lastname: Désiron
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Leumann
mesh_terms: D001386:Azides / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000032:analysis / Q000737:chemistry*; D012235:Rhodamines / Q000032:analysis / Q000737:chemistry
chemical_list: D001386:Azides; D004277:DNA, Single-Stranded; D009696:Nucleic Acids; D012235:Rhodamines; D012313:RNA
keywords: RNA diagnostics; Staudinger reaction; fluorescence sensing; homo-DNA; oligonucleotides