title: Effect of acute negative and positive energy balance on basal very-low density lipoprotein triglyceride metabolism in women.
abstract: BACKGROUND
  Acute reduction in dietary energy intake reduces very low-density lipoprotein triglyceride (VLDL-TG) concentration. Although chronic dietary energy surplus and obesity are associated with hypertriglyceridemia, the effect of acute overfeeding on VLDL-TG metabolism is not known.
  
  
  OBJECTIVE
  The aim of the present study was to investigate the effects of acute negative and positive energy balance on VLDL-TG metabolism in healthy women.
  
  
  METHODS
  Ten healthy women (AGE: 22.0±2.9 years, BMI: 21.2±1.3 kg/m(2)) underwent a stable isotopically labeled tracer infusion study to determine basal VLDL-TG kinetics after performing, in random order, three experimental trials on the previous day: i) isocaloric feeding (control) ii) hypocaloric feeding with a dietary energy restriction of 2.89±0.42 MJ and iii) hypercaloric feeding with a dietary energy surplus of 2.91±0.32 MJ. The three diets had the same macronutrient composition.
  
  
  RESULTS
  Fasting plasma VLDL-TG concentrations decreased by ∼26% after hypocaloric feeding relative to the control trial (P = 0.037), owing to decreased hepatic VLDL-TG secretion rate (by 21%, P = 0.023) and increased VLDL-TG plasma clearance rate (by ∼12%, P = 0.016). Hypercaloric feeding increased plasma glucose concentration (P = 0.042) but had no effect on VLDL-TG concentration and kinetics compared to the control trial.
  
  
  CONCLUSIONS
  Acute dietary energy deficit (∼3MJ) leads to hypotriglyceridemia via a combination of decreased hepatic VLDL-TG secretion and increased VLDL-TG clearance. On the other hand, acute dietary energy surplus (∼3MJ) does not affect basal VLDL-TG metabolism but disrupts glucose homeostasis in healthy women.
authors.affiliation: Department of Nutrition and Dietetics, Harokopio University, Athens, Greece.
authors.forename: Elena
authors.initials: E
authors.lastname: Bellou
authors.forename: Maria
authors.initials: M
authors.lastname: Maraki
authors.forename: Faidon
authors.initials: F
authors.lastname: Magkos
authors.forename: Helena
authors.initials: H
authors.lastname: Botonaki
authors.forename: Demosthenes B
authors.initials: DB
authors.lastname: Panagiotakos
authors.forename: Stavros A
authors.initials: SA
authors.lastname: Kavouras
authors.forename: Labros S
authors.initials: LS
authors.lastname: Sidossis
mesh_terms: D000328:Adult; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000378:metabolism; D014280:Triglycerides / Q000097:blood* / Q000378:metabolism; D055815:Young Adult
chemical_list: D008079:Lipoproteins, VLDL; D014280:Triglycerides; C030106:very low density lipoprotein triglyceride