title: Quercetin and epigallocatechin-3-gallate effect on the anisotropy of model membranes with cholesterol.
abstract: Cell membrane fluidity, which can be altered by oxidative stress, plays an important role in the cell physiology. Flavonoids are among the most studied food substances that prevent and/or reduce oxidative stress, but their action mechanisms are far from being understood. We performed a study on the effect of quercetin and epigallocatechin-3-gallate on 2-Dimyristoyl-sn-glycero-3-phosphocholine small unilamellar vesicles (SUVs) with different amounts of cholesterol, using Laurdan as a fluorescent probe, to put into evidence the perturbations of the phospholipid membrane fluidity and local lipid order in an attempt to decipher the action mechanism of the flavonoids at the cell membrane level. Results indicate that polyphenols modulate the transition from the gel phase to the liquid crystalline phase of SUVs in all studied membranes. SUVs with cholesterol have by themselves higher phase transition temperature and the presence of polyphenols stabilizes further the membrane. Quercetin has a dose-dependent effect on the fluidity and local order of the lipid membranes, whilst epigallocatechin-3-gallate action is not dose-dependent, the differences being attributable to the hydrophobic/hydrophilic character of the substances. The findings are discussed within the frame of earlier reports on the effect of polyphenols on artificial membranes.
authors.affiliation: Carol Davila University of Medicine and Pharmacy, Faculty of Medicine, 8 Eroii Sanitari Blvd., 050474 Bucharest, Romania. Electronic address: diana.ionescu@umf.ro.
authors.forename: Diana
authors.initials: D
authors.lastname: Ionescu
authors.forename: Denisa
authors.initials: D
authors.lastname: Margină
authors.forename: Mihaela
authors.initials: M
authors.lastname: Ilie
authors.forename: Adrian
authors.initials: A
authors.lastname: Iftime
authors.forename: Constanţa
authors.initials: C
authors.lastname: Ganea
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000737:chemistry; D007848:Laurates / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D011794:Quercetin / Q000494:pharmacology*; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D007848:Laurates; D008081:Liposomes; D010743:Phospholipids; D002392:Catechin; D002784:Cholesterol; D011794:Quercetin; C045651:epigallocatechin gallate; D015081:2-Naphthylamine; D004134:Dimyristoylphosphatidylcholine; C065580:laurdan
keywords: 2-dimyristoyl-sn-glycero-3-phosphocholine; 6-dodecanoyl-2-dimethylamino-naphthalene; Antioxidants; Chol; DMPC; DMSO; EGCG; Flavonoids; GP; Laurdan; Liposomes; Membrane anisotropy; Phase transition; QCT; SUV; anisotropy; cholesterol; dimethyl sulfoxide; epigallocatechin-3-gallate; general polarization; quercetin; r; small unilamelar vesicles