title: A continuous bovine kidney cell line constitutively expressing bovine αvβ6 integrin has increased susceptibility to foot-and-mouth disease virus.
abstract: Foot-and-mouth disease (FMD) is a worldwide problem limiting the trade of animals and their products from affected countries. The rapid isolation, serotyping, and vaccine matching of FMD virus from disease outbreaks is critical for enabling the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Some primary cells have been shown to be highly susceptible to most strains of FMD virus (FMDV) but are difficult and expensive to prepare and maintain. Since the αVβ6 integrin is a principal receptor for FMDV, we transduced a bovine kidney cell line to stably express both the αV and β6 bovine integrin subunits. This stable cell line (LFBK-αVβ6) showed β6 expression and enhanced susceptibility to FMDV infection for ≥ 100 cell passages. LFBK-αVβ6 cells were highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophilic FMDV strain O/TAW/97. In comparison to other cell types that are currently used for virus isolation, LFBK-αVβ6 cells were more effective at detecting FMDV in clinical samples, supporting their use as a more sensitive tool for virus isolation.
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: LaRocco
authors.forename: Peter W
authors.initials: PW
authors.lastname: Krug
authors.forename: Ed
authors.initials: E
authors.lastname: Kramer
authors.forename: Zaheer
authors.initials: Z
authors.lastname: Ahmed
authors.forename: Juan M
authors.initials: JM
authors.lastname: Pacheco
authors.forename: Hernando
authors.initials: H
authors.lastname: Duque
authors.forename: Barry
authors.initials: B
authors.lastname: Baxt
authors.forename: Luis L
authors.initials: LL
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000254:growth & development*; D015870:Gene Expression; D042822:Genomic Instability; D054884:Host-Pathogen Interactions; D011991:Receptors, Virus / Q000096:biosynthesis* / Q000235:genetics; D019030:Receptors, Vitronectin / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014161:Transduction, Genetic; D014776:Virus Cultivation / Q000379:methods
chemical_list: D011991:Receptors, Virus; D019030:Receptors, Vitronectin; D011994:Recombinant Proteins; C064660:integrin alphaVbeta5