title: Automated identification of multiple seizure-related and interictal epileptiform event types in the EEG of mice.
abstract: Visual scoring of murine EEG signals is time-consuming and subject to low inter-observer reproducibility. The Racine scale for behavioral seizure severity does not provide information about interictal or sub-clinical epileptiform activity. An automated algorithm for murine EEG analysis was developed using total signal variation and wavelet decomposition to identify spike, seizure, and other abnormal signal types in single-channel EEG collected from kainic acid-treated mice. The algorithm was validated on multi-channel EEG collected from γ-butyrolacetone-treated mice experiencing absence seizures. The algorithm identified epileptiform activity with high fidelity compared to visual scoring, correctly classifying spikes and seizures with 99% accuracy and 91% precision. The algorithm correctly identifed a spike-wave discharge focus in an absence-type seizure recorded by 36 cortical electrodes. The algorithm provides a reliable and automated method for quantification of multiple classes of epileptiform activity within the murine EEG and is tunable to a variety of event types and seizure categories.
authors.affiliation: Neurobiology of Disease PhD Program, Mayo Graduate School, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Bergstrom
authors.forename: Jee Hyun
authors.initials: JH
authors.lastname: Choi
authors.forename: Armando
authors.initials: A
authors.lastname: Manduca
authors.forename: Hee-Sup
authors.initials: HS
authors.lastname: Shin
authors.forename: Greg A
authors.initials: GA
authors.lastname: Worrell
authors.forename: Charles L
authors.initials: CL
authors.lastname: Howe
mesh_terms: D000465:Algorithms; D000818:Animals; D001331:Automation; D001522:Behavior, Animal; D058256:Brain Waves; D003292:Convulsants / Q000633:toxicity; D004569:Electroencephalography; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006624:Hippocampus / Q000503:physiopathology*; D007608:Kainic Acid / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D015588:Observer Variation; D012640:Seizures / Q000139:chemically induced / Q000145:classification / Q000175:diagnosis / Q000503:physiopathology*; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014741:Video Recording; D058067:Wavelet Analysis
chemical_list: D003292:Convulsants; D007608:Kainic Acid