title: Green synthesis, characterization and antimicrobial activity of Au NPs using Euphorbia hirta L. leaf extract.
abstract: The activity of a nano sized particle is said to be greater when compared to that of its parent materials combined. Thus, an attempt was made to produce gold nanostructures having unusual physicochemical properties. In this study, eco-friendly, non-toxic gold nanoparticles (Au NPs) were biologically synthesized using the leaf extract of Euphorbia hirta L. The synthesis of Au NPs was confirmed by a change in extract color from pale yellow to purple and surface plasmon resonance spectra obtained in a range of approximately 530nm. Nanoparticles whose sizes ranged from 6nm to 71nm, were synthesized. Different instrumental techniques were used to characterize the synthesized AuNPs, such TEM, XRD, EDAX, AFM, particle size analyzer, FTIR and Raman spectra. Also the antibacterial activity of the green synthesized Au NPs against bacterial strains of Escherchia coli, Pseudomonas aeroginosa and Klebsiella pneumonia was studied using MIC method, and found to be highly effective.
authors.affiliation: Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Karunya Nagar, Coimbatore, Tamilnadu, India. aannamalai2001@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: Annamalai
authors.forename: V L P
authors.initials: VL
authors.lastname: Christina
authors.forename: D
authors.initials: D
authors.lastname: Sudha
authors.forename: M
authors.initials: M
authors.lastname: Kalpana
authors.forename: P T V
authors.initials: PT
authors.lastname: Lakshmi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D028481:Euphorbia / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D006046:Gold