title: Enhanced maturation and function of dendritic cells using hydrogel coated plate and antigen electroporation.
abstract: Dendritic cells (DCs) are potent antigen-presenting cells that can be matured in vitro from immature dendritic cells (iDCs) in the presence of several biological agents such as cytokine cocktail, CD40L, TNF-a and antigen loading, which are necessary and achieved using various protocols, such as lipofection, passive pulse or electroporation. However, these DCs maturation protocols may cause with a significant loss of cells because of cellular attachment and spreading during culturing. Some biomaterials that influence adhesion and development of cells have been used in cell culture techniques, and it was thought that they might be applied on the culture of DCs. In this study, we used polyHEMA, which is a hydrogel coating biomaterial that prevents DCs from adherence, and investigated whether hydrogel coating affects the maturation of iDCs. The efficiency in the generation of mDCs was improved through hydrogel coating procedure and a dendritic cell maturation marker, CD83, was significantly increased in hydrogel-coated culture condition. The antigen-loaded mDCs from electroporation were further expressed the CD83. The mDCs generated in the hydrogel-coated culture condition showed more, longer and thicker dendrites, and produced more amounts of cytokines such as IL-12 and IFN-γ. Therefore, it was suggested that the hydrogel-coated culture condition could improve function of mDCs. Cheol-Hun Son and Jae-Ho Bae contributed equally to this work.
authors.affiliation: Department of Research Center, Dongnam Institute of Radiological & Medical Sciences, Busan 619-953, South Korea.
authors.forename: Cheol-Hun
authors.initials: CH
authors.lastname: Son
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Bae
authors.forename: Dong Yeok
authors.initials: DY
authors.lastname: Shin
authors.forename: Hong-Rae
authors.initials: HR
authors.lastname: Lee
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
authors.forename: Kwangmo
authors.initials: K
authors.lastname: Yang
authors.forename: You-Soo
authors.initials: YS
authors.lastname: Park
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D000941:Antigens / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D018274:Electroporation; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D020014:K562 Cells; D011102:Polyhydroxyethyl Methacrylate / Q000494:pharmacology*
chemical_list: D000906:Antibodies; D000941:Antigens; D018664:Interleukin-12; D011102:Polyhydroxyethyl Methacrylate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007371:Interferon-gamma