title: Investigation of transcriptional responses of juvenile mouse bone marrow to power frequency magnetic fields.
abstract: To seek alterations in gene transcription in bone marrow cells following in vivo exposure of juvenile mice to power frequency magnetic fields, young (21-24-day old) C57BL/6 mice were exposed to a 100μT 50Hz magnetic field for 2h. Transcription was analysed by three methods, High Coverage Expression Profiling (HiCEP), Illumina microarrays and quantitative real-time polymerase chain reaction (QRT-PCR). A pilot HiCEP experiment with 6 exposed (E) and 6 non-exposed (NE) mice identified four candidate responsive transcripts (two unknown transcripts (AK152075 and F10-NED), phosphatidylinositol binding clathrin assembly protein (Picalm) and exportin 7 (Xpo7)). A larger experiment compared 19 E and 15 NE mice using two independent QRT-PCR assays and repeated microarray assays. No significant field-dependent changes were seen, although Picalm showed a trend to significance in one QRT-PCR assay (E/NE=0.91; P=0.06). However, the study was underpowered to detect an effect of this magnitude (52% power at P=0.05). These data indicate the current experimental constraints in detecting small changes in transcription that may occur in response to magnetic fields. These constraints result from technical limitations in the accuracy of assays and biological variation, which together were sufficient to account statistically for the number of differentially expressed transcripts identified in the pilot experiment.
authors.affiliation: Health Protection Agency Centre for Radiation, Chemical and Environmental Hazards, Chilton, Didcot, Oxfordshire, UK.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kabacik
authors.forename: Heide
authors.initials: H
authors.lastname: Kirschenlohr
authors.forename: Claudine
authors.initials: C
authors.lastname: Raffy
authors.forename: Kevin
authors.initials: K
authors.lastname: Whitehill
authors.forename: Margaret
authors.initials: M
authors.lastname: Coster
authors.forename: Masumi
authors.initials: M
authors.lastname: Abe
authors.forename: Kevin
authors.initials: K
authors.lastname: Brindle
authors.forename: Christophe
authors.initials: C
authors.lastname: Badie
authors.forename: Zenon
authors.initials: Z
authors.lastname: Sienkiewicz
authors.forename: Simon
authors.initials: S
authors.lastname: Bouffler
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D060526:Magnetic Fields / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D034041:Monomeric Clathrin Assembly Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010865:Pilot Projects; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D034041:Monomeric Clathrin Assembly Proteins; C573094:PICALM protein, mouse