title: A trans-acting ketoreductase in biosynthesis of a symmetric polyketide dimer SIA7248.
abstract: NEW ASSEMBLY LINE, NEW COMPOUND: SIA7248, a new symmetric macrolide, was isolated from a marine-derived Streptomyces strain. Bioinformatic analyses of the identified biosynthetic gene cluster (sia) for SIA7248 suggested a polyketide biosynthesis utilizing an iteratively trans-acting ketoreductase (KR). We characterized SiaM as a trans-KR to catalyse reductions of various β-ketoacyl-thioesters with D-stereospecificity.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zou
authors.forename: Haixing
authors.initials: H
authors.lastname: Yin
authors.forename: Dekun
authors.initials: D
authors.lastname: Kong
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Shuangjun
authors.initials: S
authors.lastname: Lin
mesh_terms: D018360:Crystallography, X-Ray; D019281:Dimerization; D008958:Models, Molecular; D005810:Multigene Family; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D061065:Polyketides; D010088:Oxidoreductases