title: Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation.
abstract: A method is described in which the interference of radiated second-harmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of the observed second-harmonic generation intensity, which is described by a simple model that accounts for the interferences of the radiated fields. The technique presented shows promise for future experiments investigating structural changes induced by the formation of a DNA-biomolecule complex.
authors.affiliation: Department of Chemistry, Columbia University, New York, NY 10027, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Doughty
authors.forename: Yi
authors.initials: Y
authors.lastname: Rao
authors.forename: Samuel W
authors.initials: SW
authors.lastname: Kazer
authors.forename: Sheldon J J
authors.initials: SJ
authors.lastname: Kwok
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turro
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Eisenthal
mesh_terms: D001703:Biophysics / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D003630:Daunorubicin / Q000494:pharmacology; D008024:Ligands; D015233:Models, Statistical; D009690:Nucleic Acid Conformation; D055095:Optics and Photonics; D009991:Oscillometry / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
chemical_list: D008024:Ligands; D004247:DNA; D003630:Daunorubicin