title: Association of interleukin-17F gene polymorphism with enterovirus 71 encephalitis in patients with hand, foot, and mouth disease.
abstract: Enterovirus 71 (EV71) is one of the common pathogenic agents of hand, foot, and mouth disease (HFMD) and is associated with severe complications including encephalitis. Interleukin (IL)-17F plays an important role in tissue inflammation by inducing release of proinflammatory cytokines and chemokines. We investigated the association between EV71 encephalitis and of IL-17F 7488T/C (rs763780) gene polymorphism, which is known to cause a His-to-Arg substitution at amino acid 161. The study was performed in 58 Chinese patients with EV71 encephalitis and 127 Chinese patients with EV71-related HFMD without complications. Genotyping was determined by the polymerase chain reaction-restriction fragment length polymorphism technique. The patients with EV71 encephalitis had a significantly lower frequency of the IL-17F 7488TC+CC genotypes (10.3%) as compared to the patients with EV71-related HFMD without complications (27.6%, p = 0.008). The frequency of IL-17F 7488C alleles was also significantly lower among the patients with EV71 encephalitis (5.2%) as compared to that of the patients with EV71-related HFMD without complications (15%, OR = 0.310, 95% CI = 0.127-0.756, p = 0.006). Furthermore, homozygotes with the T allele had significantly higher levels of C-reactive protein, white blood cell count, and neutrophil count as compared to the patients with CC+CT genotypes (p = 0.004, 0.001, and 0.000, respectively). These findings suggested that the IL-17F 7488C allele could be significantly associated with protection against encephalitis in Chinese patients with EV71-related HFMD.
authors.affiliation: Pediatric Department, Affiliated Hospital of Qingdao University Medical School, 16 Jangsu road, Qingdao, 266100, Shandong, China.
authors.forename: Tiegang
authors.initials: T
authors.lastname: Lv
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Zhenliang
authors.initials: Z
authors.lastname: Han
authors.forename: Zongbo
authors.initials: Z
authors.lastname: Chen
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002681:China; D018792:Encephalitis, Viral / Q000150:complications / Q000235:genetics*; D029821:Enterovirus A, Human; D004769:Enterovirus Infections / Q000150:complications / Q000235:genetics*; D005260:Female; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D007223:Infant; D020381:Interleukin-17 / Q000235:genetics*; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D011110:Polymorphism, Genetic
chemical_list: C440021:IL17F protein, human; D020381:Interleukin-17; D002097:C-Reactive Protein