title: The mycotoxin T-2 inhibits hepatic cytochrome P4503A activity in pigs.
abstract: Mycotoxins are toxic metabolites produced by fungi that readily colonize crops. After ingestion, these mycotoxins can compromise intestinal health, and once entering the blood stream, even affect the liver and its metabolizing enzymes. It was therefore the aim of the present study to investigate the effect of T-2 toxin, an emerging and potent Fusarium mycotoxin, on the enzymatic activity of cytochrome P4503A (CYP3A) metabolizing enzymes in the liver of pigs. In addition, a yeast-derived feed additive that claims to bind T-2 toxin was included in the study to evaluate its efficacy. Our results demonstrated that a 14-days intake of T-2 toxin contaminated feed at a dose of 903 μg/kg feed, whether or not combined with the mycotoxin binder, results in a substantial inhibition of the CYP3A activity in the liver of pigs. This result may be of importance for animal health, the pharmacokinetics and the withdrawal time of drugs that are substrate of CYP3A enzymes, and consequently can be a threat for public health with respect to tissue residues of these drugs.
authors.affiliation: Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. joline.goossens@ugent.be
authors.forename: J
authors.initials: J
authors.lastname: Goossens
authors.forename: L
authors.initials: L
authors.lastname: De Bock
authors.forename: A
authors.initials: A
authors.lastname: Osselaere
authors.forename: E
authors.initials: E
authors.lastname: Verbrugghe
authors.forename: M
authors.initials: M
authors.lastname: Devreese
authors.forename: K
authors.initials: K
authors.lastname: Boussery
authors.forename: J
authors.initials: J
authors.lastname: Van Bocxlaer
authors.forename: P
authors.initials: P
authors.lastname: De Backer
authors.forename: S
authors.initials: S
authors.lastname: Croubels
mesh_terms: D000821:Animal Feed; D000818:Animals; D065692:Cytochrome P-450 CYP3A Inhibitors; D005506:Food Contamination; D005670:Fusarium / Q000737:chemistry; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D013552:Swine; D013605:T-2 Toxin / Q000633:toxicity*
chemical_list: D065692:Cytochrome P-450 CYP3A Inhibitors; D013605:T-2 Toxin