title: Bacillus subtilis mutants with knockouts of the genes encoding ribonucleases RNase Y and RNase J1 are viable, with major defects in cell morphology, sporulation, and competence.
abstract: The genes encoding the ribonucleases RNase J1 and RNase Y have long been considered essential for Bacillus subtilis cell viability, even before there was concrete knowledge of their function as two of the most important enzymes for RNA turnover in this organism. Here we show that this characterization is incorrect and that ΔrnjA and Δrny mutants are both viable. As expected, both strains grow relatively slowly, with doubling times in the hour range in rich medium. Knockout mutants have major defects in their sporulation and competence development programs. Both mutants are hypersensitive to a wide range of antibiotics and have dramatic alterations to their cell morphologies, suggestive of cell envelope defects. Indeed, RNase Y mutants are significantly smaller in diameter than wild-type strains and have a very disordered peptidoglycan layer. Strains lacking RNase J1 form long filaments in tight spirals, reminiscent of mutants of the actin-like proteins (Mre) involved in cell shape determination. Finally, we combined the rnjA and rny mutations with mutations in other components of the degradation machinery and show that many of these strains are also viable. The implications for the two known RNA degradation pathways of B. subtilis are discussed.
authors.affiliation: CNRS UPR 9073, Université Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Figaro
authors.forename: Sylvain
authors.initials: S
authors.lastname: Durand
authors.forename: Laetitia
authors.initials: L
authors.lastname: Gilet
authors.forename: Nadège
authors.initials: N
authors.lastname: Cayet
authors.forename: Martin
authors.initials: M
authors.lastname: Sachse
authors.forename: Ciarán
authors.initials: C
authors.lastname: Condon
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012260:Ribonucleases