title: Detection of type A trichothecene di-glucosides produced in corn by high-resolution liquid chromatography-Orbitrap mass spectrometry.
abstract: The existence of di-glucosylated derivative of T-2 toxin in plant (corn powder) was confirmed for the first time in addition to that of HT-2 toxin. These masked mycotoxins (mycotoxin glucosides) were identified as T-2 toxin-di-glucoside (T2GlcGlc) and HT-2 toxin-di-glucoside (HT2GlcGlc) based on accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometric (LC-Orbitrap MS) analysis. Although the absolute structure of T2GlcGlc was not clarified, two glucose molecules were suggested to be conjugated at 3-OH position in tandem when considering the structure of T-2 toxin. On the other hand, the specification of the structure seems to be more complicated in the case of HT2GlcGlc, since HT-2 toxin has two possible positions (at 3-OH and 4-OH) to be glusocylated. In addition, 15-monoacetoxyscirpenol-glucoside (MASGlc) was also detected in the identical sample.
authors.affiliation: National Agriculture and Food Research Organization (NARO), National Food Research Institute, Tsukuba-shi, Ibaraki, Japan. hironkgw@affrc.go.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sakamoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sago
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagashima
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005960:Glucosides / Q000302:isolation & purification*; D007536:Isomerism; D015394:Molecular Structure; D011208:Powders; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology
chemical_list: D005960:Glucosides; D011208:Powders; D013605:T-2 Toxin; C012351:HT-2 toxin