title: Lupane-type triterpenoidal saponins from Pulsatilla chinensis and their anticomplement activities through the classical pathway.
abstract: Four (1-4) previously unknown lupane-type triterpenoidal saponins were isolated from the roots of Pulsatilla chinensis, along with six triterpene saponins (5-10). The structures of saponins 1-4 were determined as 23-hydroxy-3β-[(O-β-D-glucopyranosyl-(1 → 4)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (1), 23-hydroxy-3β-[(O-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 4)]-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (2), 3β-[(O-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (3), and 3β-[(O-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid 28-O-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (4), on the basis of hydrolysis and spectral evidence, including 1D- and 2D-NMR and HR-ESI-MS analyses. These pure isolates (1-10) were tested for their anticomplement activity, using an in vitro assay of the complement system of the classical pathway.
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, China.
authors.forename: Qiong-ming
authors.initials: QM
authors.lastname: Xu
authors.forename: Zhan
authors.initials: Z
authors.lastname: Shu
authors.forename: Wei-feng
authors.initials: WF
authors.lastname: Zhu
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Liu
authors.forename: Xiao-ran
authors.initials: XR
authors.lastname: Li
authors.forename: Shi-lin
authors.initials: SL
authors.lastname: Yang
mesh_terms: D000818:Animals; D003169:Complement Inactivator Proteins / Q000187:drug effects*; D003171:Complement Pathway, Classical; D006168:Guinea Pigs; D010936:Plant Extracts / Q000737:chemistry; D031946:Pulsatilla / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D053978:Pentacyclic Triterpenes
chemical_list: D003169:Complement Inactivator Proteins; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C012749:anticomplement; C480546:lupane; D053978:Pentacyclic Triterpenes