title: Conversion of acid hydrolysate of oil palm empty fruit bunch to L-lactic acid by newly isolated Bacillus coagulans JI12.
abstract: Cost-effective conversion of lignocellulose hydrolysate to optically pure lactic acid is commercially attractive but very challenging. Bacillus coagulans JI12 was isolated from natural environment and used to produce L-lactic acid (optical purity > 99.5 %) from lignocellulose sugars and acid hydrolysate of oil palm empty fruit bunch (EFB) at 50 °C and pH 6.0 without sterilization of the medium. In fed-batch fermentation with 85 g/L initial xylose and 55 g/L xylose added after 7.5 h, 137.5 g/L lactic acid was produced with a yield of 98 % and a productivity of 4.4 g/L h. In batch fermentation of a sugar mixture containing 8.5 % xylose, 1 % glucose, and 1 % L-arabinose, the lactic acid yield and productivity reached 98 % and 4.8 g/L h, respectively. When EFB hydrolysate was used, 59.2 g/L of lactic acid was produced within 9.5 h at a yield of 97 % and a productivity of 6.2 g/L h, which are the highest among those ever reported from lignocellulose hydrolysates. These results indicate that B. coagulans JI12 is a promising strain for industrial production of L-lactic acid from lignocellulose hydrolysate.
authors.affiliation: Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A STAR), 1 Pesek Road, Jurong Island, Singapore 627833, Singapore.
authors.forename: Lidan
authors.initials: L
authors.lastname: Ye
authors.forename: Mohammad Sufian Bin
authors.initials: MS
authors.lastname: Hudari
authors.forename: Xingding
authors.initials: X
authors.lastname: Zhou
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Jin Chuan
authors.initials: JC
authors.lastname: Wu
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005285:Fermentation; D005638:Fruit / Q000737:chemistry; D019344:Lactic Acid / Q000378:metabolism*; D008031:Lignin / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D019344:Lactic Acid; D008031:Lignin