title: Strategies for the discovery and engineering of enzymes for biocatalysis.
abstract: Protein engineering is the most important method to overcome the limitations of natural enzymes as biocatalysts. The past few years have seen a tremendous increase in novel concepts to facilitate the design of mutant libraries for focused directed evolution mostly guided by advanced bioinformatic tools. In addition, advanced high-throughput methods were developed using, for example, FACS analysis or microfluidic systems. These achievements significantly facilitate the tailor-made design of enzymes to make them suitable for industrial applications.
authors.affiliation: Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Davids
authors.forename: Marlen
authors.initials: M
authors.lastname: Schmidt
authors.forename: Dominique
authors.initials: D
authors.lastname: Böttcher
authors.forename: Uwe T
authors.initials: UT
authors.lastname: Bornscheuer
mesh_terms: D055162:Biocatalysis; D019295:Computational Biology; D030562:Databases, Protein; D019020:Directed Molecular Evolution; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D057166:High-Throughput Screening Assays; D015202:Protein Engineering / Q000379:methods*; D012984:Software
chemical_list: D004798:Enzymes