title: Ciprofloxacin is actively transported across bronchial lung epithelial cells using a Calu-3 air interface cell model.
abstract: Ciprofloxacin is a well-established broad-spectrum fluoroquinolone antibiotic that penetrates well into the lung tissues; still, the mechanisms of its transepithelial transport are unknown. The contributions of specific transporters, including multidrug efflux transporters, organic cation transporters, and organic anion-transporting polypeptide transporters, to the uptake of ciprofloxacin were investigated in vitro using an air interface bronchial epithelial model. Our results demonstrate that ciprofloxacin is subject to predominantly active influx and a slight efflux component.
authors.affiliation: Respiratory Technology, Woolcock Institute of Medical Research, Sydney, NSW, Australia.
authors.forename: Hui Xin
authors.initials: HX
authors.lastname: Ong
authors.forename: Daniela
authors.initials: D
authors.lastname: Traini
authors.forename: Mary
authors.initials: M
authors.lastname: Bebawy
authors.forename: Paul M
authors.initials: PM
authors.lastname: Young
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001693:Biological Transport, Active; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002460:Cell Line; D002939:Ciprofloxacin / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008954:Models, Biological; D027361:Organic Anion Transporters / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; C450859:SLCO2B1 protein, human; D002939:Ciprofloxacin