title: Contribution of molecular diagnosis to congenital toxoplasmosis.
abstract: We evaluated the performance of three real-time polymerase chain reaction (PCR) assays on 73 samples from mothers and children with congenital toxoplasmosis. PCR assays had significantly higher sensitivity in prenatal period than in birth period when targeting the 529-bp repeat element (81.3% versus 36.0%) or the B1 gene (64.6% versus 20.0%).
authors.affiliation: Mycology-Parasitology Laboratory, EA4547-BDEEP, CIIL, Faculté de Médecine, Univ Nord de France (UDSL), CHRU de Lille, France. laurence.delhaes@chru-lille.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Delhaes
authors.forename: Hélène
authors.initials: H
authors.lastname: Yera
authors.forename: Sandrine
authors.initials: S
authors.lastname: Ache
authors.forename: Vassili
authors.initials: V
authors.lastname: Tsatsaris
authors.forename: Véronique
authors.initials: V
authors.lastname: Houfflin-Debarge
mesh_terms: D002648:Child; D016054:DNA, Protozoan / Q000302:isolation & purification; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis*; D011296:Prenatal Diagnosis / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis*
chemical_list: D016054:DNA, Protozoan; D007074:Immunoglobulin G