title: Prevalence of blaPER-1 and integrons in ceftazidime-resistant Gram-negative bacteria at a university hospital in Turkey.
abstract: In this study, we examined the prevalence of the PER-1 enzyme and the presence of clinically important integron classes in ceftazidime-resistant Gram-negative bacteria isolated at a university hospital. The blaPER-1 gene was detected by PCR in 33 (19.5%) of the total 169 Gram-negative bacteria, including 17 (23.3%) of the 73 Pseudomonas aeruginosa isolates and 16 (25%) of the 64 Acinetobacter baumannii complex isolates. Molecular fingerprinting revealed that blaPER-1 prevalence was mostly due to the dissemination of clonally related P. aeruginosa and A. baumannii complex strains. Class 1 integron (intI1) was detected in 52.7% of the 169 bacteria examined in this study. Its detection rates were estimated at 49.3% and 57.8% of the P. aeruginosa and A. baumannii complex strains isolated, respectively. It was also detected in 11 of the 16 Escherichia coli isolates and 5 of the 10 Klebsiella pneumoniae isolates. A single E. coli isolate and another K. pneumoniae isolate contained both class 1 and class 2 integrase genes. The results of this study revealed that clonally related blaPER-1-positive P. aeruginosa and A. baumannii complex strains, mostly harboring intI1, had disseminated at our hospital.
authors.affiliation: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey. eracb＠yahoo.com
authors.forename: Bayrı
authors.initials: B
authors.lastname: Eraç
authors.forename: Mine
authors.initials: M
authors.lastname: Hoşgör-Limoncu
authors.forename: Şafak
authors.initials: Ş
authors.lastname: Ermertcan
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Taşlı
authors.forename: Şöhret
authors.initials: Ş
authors.lastname: Aydemir
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D006785:Hospitals, University; D006801:Humans; D032023:Integrons; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014421:Turkey / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002442:Ceftazidime; C108863:beta-lactamase PER-1; D001618:beta-Lactamases