title: Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.
abstract: On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.
authors.affiliation: Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden.
authors.forename: A Ahmad
authors.initials: AA
authors.lastname: Tajudin
authors.forename: K
authors.initials: K
authors.lastname: Petersson
authors.forename: A
authors.initials: A
authors.lastname: Lenshof
authors.forename: A-M
authors.initials: AM
authors.lastname: Swärd-Nilsson
authors.forename: L
authors.initials: L
authors.lastname: Aberg
authors.forename: G
authors.initials: G
authors.lastname: Marko-Varga
authors.forename: J
authors.initials: J
authors.lastname: Malm
authors.forename: H
authors.initials: H
authors.lastname: Lilja
authors.forename: T
authors.initials: T
authors.lastname: Laurell
mesh_terms: D000162:Acoustics; D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D017430:Prostate-Specific Antigen / Q000097:blood*
chemical_list: D015415:Biomarkers; D017430:Prostate-Specific Antigen