title: Crystallization and preliminary X-ray crystallographic analysis of recombinant β-mannosidase from Aspergillus niger.
abstract: β-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal β-linked mannoside in various oligomeric saccharide structures. β-Mannosidase from Aspergillus niger was expressed in Pichia pastoris and purified to clear homogeneity. β-Mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 Å resolution. The crystal belonged to space group P1, with unit-cell parameters a=62.37, b=69.73, c=69.90 Å, α=108.20, β=101.51, γ=103.20°. The parameters derived from the data collection indicate the presence of one molecule in the asymmetric unit.
authors.affiliation: Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Demo
authors.forename: Barbora
authors.initials: B
authors.lastname: Fliedrová
authors.forename: Lenka
authors.initials: L
authors.lastname: Weignerová
authors.forename: Michaela
authors.initials: M
authors.lastname: Wimmerová
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry* / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D008358:Mannose / Q000737:chemistry*; D010843:Pichia / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D044902:beta-Mannosidase / Q000737:chemistry* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D044902:beta-Mannosidase; D008358:Mannose
keywords: Aspergillus niger; β-mannosidase