title: Pharmacokinetic and pharmacodynamic studies of two different rabbit antithymocyte globulin dosing regimens: results of a randomized trial.
abstract: Rabbit antithymocyte globulin (rATG; Thymoglobulin(®)) is currently used to prevent acute rejection in kidney transplantation. The dose and regimen of rATG have not been optimized. Moreover, the impact of different treatment regimens on T-cell phenotype reconstitution remains unknown. We conducted a prospective randomized study of 17 renal transplant patients to determine the pharmacokinetics of total and active (bound to human cells) rATG and T-cell phenotype reconstitution after rATG administration. Patients received rATG at a total dose of 6mg/kg, administered either as 1.5mg/kg/day on days 0-3 (Group 1, n=8) or 3mg/kg on days 0 and 3 (Group 2, n=9). All patients received tacrolimus, mycophenolate mofetil and steroids. Blood samples were assayed for total rATG by enzyme linked immunosorbent assay and active rATG by flow cytometry. Maximum concentrations and terminal half-lives were similar between the two groups but at month 3 Group 1 had significantly lower values for total rATG (concentration was 6.2±1.1μg/mL versus 10.2±2.9μg/mL in Group 2, p=0.027) and total rATG dose-normalized AUC (374±83dayg/mL versus 508±149dayg/mL in Group 2, p=0.046). Time to sub-therapeutic levels (<1μg/mL) of active rATG was significantly shorter in Group 1 (18.75±6.9days versus 20±7.5days in Group 2, p<0.001). rATG induced significant depletion followed by slow reconstitution of CD3(+), CD4(+) and CD8(+) cells, with no marked differences between groups. B-cell count was unaffected, whereas CD3(-)CD56(+) NK-cell depletion was observed in both groups. rATG induced a significant decrease in the proportion of naïve CD4(+) T-cells, which plateaued after month 1 in Group 1 and after month 6 in Group 2. The proportion of central memory CD4(+) T-cells increased to a similar extent in both groups (Group 1: 38±18% at baseline, 74±23% at one year, p=0.009; Group 2: 32±14% at baseline, 65±14% at one year, p=0.001). In conclusion, our results suggest that the dosing regimen for rATG induction influences pharmacokinetic parameters without affecting the quality of immune reconstitution.
authors.affiliation: Université François Rabelais, Tours, France; CHRU Tours, Département de Néphrologie et Immunologie Clinique, Tours, France.
authors.forename: Matthias
authors.initials: M
authors.lastname: Büchler
authors.forename: Hélène
authors.initials: H
authors.lastname: Longuet
authors.forename: Roxane
authors.initials: R
authors.lastname: Lemoine
authors.forename: Florence
authors.initials: F
authors.lastname: Herr
authors.forename: Philippe
authors.initials: P
authors.lastname: Gatault
authors.forename: Gilles
authors.initials: G
authors.lastname: Thibault
authors.forename: David
authors.initials: D
authors.lastname: Ternant
authors.forename: Christine
authors.initials: C
authors.lastname: Foulon
authors.forename: Bernadette
authors.initials: B
authors.lastname: Pilorge
authors.forename: Djamila
authors.initials: D
authors.lastname: Lemay
authors.forename: Crystal
authors.initials: C
authors.lastname: Sung
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Halimi
authors.forename: Christophe
authors.initials: C
authors.lastname: Baron
authors.forename: Yvon
authors.initials: Y
authors.lastname: Lebranchu
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D007156:Immunologic Memory / Q000187:drug effects; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000961:Antilymphocyte Serum