title: Using Xenopus skin to study cilia development and function.
abstract: Cilia are prevalent biological structures that are important for cell signaling and for generating fluid flow (or motility). Cilia are found throughout biology from single-celled organisms to vertebrates, and many model systems have been employed for their analysis. Here, we describe the use of Xenopus larval skin as a system for the study of ciliogenesis and ciliary function. In particular, we describe basic molecular and embryological manipulations and imaging techniques that have proven particularly useful for understanding the polarized beating of cilia and the generation of directed fluid flow (Werner & Mitchell, 2012). However, these same tools have the potential to benefit a large number of cilia-related biological questions.
authors.affiliation: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Werner
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D002923:Cilia / Q000378:metabolism* / Q000502:physiology*; D015398:Signal Transduction; D012867:Skin / Q000166:cytology*; D014981:Xenopus