title: A controllable gene expression system in liposomes that includes a positive feedback loop.
abstract: We introduced a positive feedback loop into a LacI-dependent gene expression system in lipid vesicles, producing a cell-like system that senses and responds to an external signal with a high signal-to-noise ratio. This fully reconstituted system will be a useful tool in future applications in in vitro synthetic biology.
authors.affiliation: Graduate School of Information Science and Technology, Osaka University, Osaka 565-0871, Japan.
authors.forename: Shungo
authors.initials: S
authors.lastname: Kobori
authors.forename: Norikazu
authors.initials: N
authors.lastname: Ichihashi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kazuta
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D015374:Biosensing Techniques; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D025461:Feedback, Physiological; D015870:Gene Expression; D005818:Genetic Engineering; D007544:Isopropyl Thiogalactoside / Q000737:chemistry; D056968:Lac Repressors / Q000235:genetics; D008081:Liposomes / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D056968:Lac Repressors; C539064:LacI protein, E coli; D008081:Liposomes; D007544:Isopropyl Thiogalactoside