title: The role of lipoic acid in the protection against of metallic pollutant effects in the shrimp Litopenaeus vannamei (Crustacea, Decapoda).
abstract: The effects of cadmium (Cd) and arsenic (As), dosed alone or in combination have been poorly investigated in crustaceans. Besides, it is not known if dietary supplementation of exogenous antioxidants, like lipoic acid (LA), might prevent or even reverse toxic effects of Cd and As. The objective of the present study was to evaluate the role of lipoic acid in modulating biochemical responses after Cd and As exposures in Litopenaeus vannamei. Muscle from shrimp exposed to Cd alone or Cd+As showed a decrease in glutathione (GSH) levels, while the pre-treatment with LA reversed this situation. In this tissue, the pre-treatment with LA also induced an increase in glutathione-S-transferase (GST) activity in all groups. In hepatopancreas it was observed a marked accumulation of Cd and As, a decrease in the reactive oxygen species (ROS) concentration in response to Cd exposure alone (-LA); concomitant in the same group it was observed an increment of metallothionein-like content. As exposure induced an increase in GSH levels but LA reversed this increase. Also, LA showed to increase the GST activity in all groups treated. Besides, in this organ LA showed to augment total antioxidant competence. Obtained results indicate that LA can be used as a chemo-protectant against oxidative insults in shrimp.
authors.affiliation: Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande-FURG, Rio Grande, RS, Brazil.
authors.forename: Roberta Oliveira
authors.initials: RO
authors.lastname: Lobato
authors.forename: Silvana Manske
authors.initials: SM
authors.lastname: Nunes
authors.forename: Wilson
authors.initials: W
authors.lastname: Wasielesky
authors.forename: Daniele
authors.initials: D
authors.lastname: Fattorini
authors.forename: Francesco
authors.initials: F
authors.lastname: Regoli
authors.forename: José Marìa
authors.initials: JM
authors.lastname: Monserrat
authors.forename: Juliane
authors.initials: J
authors.lastname: Ventura-Lima
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001151:Arsenic / Q000633:toxicity*; D002104:Cadmium / Q000633:toxicity*; D003445:Crustacea / Q000187:drug effects*; D019587:Dietary Supplements; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D033561:Penaeidae / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D008063:Thioctic Acid / Q000008:administration & dosage*; D014874:Water Pollutants, Chemical
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D002104:Cadmium; D008063:Thioctic Acid; D005978:Glutathione; D001151:Arsenic