title: Isolation of low-molecular albumins of 2S fraction from soybean (Glycine max (L.) Merrill).
abstract: Numerous studies have shown that consumption of soybean products decrease the risk of cancers in humans. Experiments at the molecular level have demonstrated that in most cases proteins and peptides are responsible for the anticancer properties of soybeen. Special attention should be paid to lunasin - a peptide described for the first time 16 years ago. Due to its structure it causes i.a., inhibition of cancer cell proliferation. A novel procedure for the isolation and purification of low-molecular-mass 2S soybean albumin protein is described in the present paper. A fraction of four peptides one of them corresponding to molecular mass and isoelectric point characteristic for lunasin. The obtained peptides decreased on the rate of HeLa cell proliferation.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agronomy and Bioengineering, University of Life Sciences, Poznań, Poland. mariolagalbas@gmail.com
authors.forename: Mariola
authors.initials: M
authors.lastname: Galbas
authors.forename: Filip
authors.initials: F
authors.lastname: Porzucek
authors.forename: Anna
authors.initials: A
authors.lastname: Woźniak
authors.forename: Ryszard
authors.initials: R
authors.lastname: Słomski
authors.forename: Marek
authors.initials: M
authors.lastname: Selwet
mesh_terms: D000418:Albumins / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D030262:Soybean Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000418:Albumins; D000970:Antineoplastic Agents; C120514:GM2S-1 protein, soybean; D030262:Soybean Proteins