title: Synaptic adhesion molecules in Cadm family at the neuromuscular junction.
abstract: RA175/SynCAM1/Cadm1 (Cadm1), a member of the immunoglobulin superfamily, is a synaptic cell adhesion molecule that has a PDZ-binding motif at the C-terminal region. It promotes the formation of presynaptic terminals and induces functional synapses in the central nervous system. Cadm1-deficient (knockout [KO]) mice show behavioral abnormalities, including excessive aggression and anxiety, but do not show any symptoms of neuromuscular disorder, although neuromuscular junctions (NMJs) have structures similar to synapses. We have examined the expression of members of the Cadm family in the mouse muscle tissues. Cadm4 and Cadm1 were major components of the Cadm family, and Cadm3 was faintly detected, but Cadm2 was not detected by RT-PCR. Cadm4 as well as Cadm1 colocalized with alpha-bungarotoxin at the NMJs and interacted with the multiple PDZ domain protein Mupp1. Cadm4 was expressed in Cadm1-KO mice and might compensate for Cadm1 loss through interactions with Mupp1.
authors.affiliation: Center for Medical Science, International University of Health and Welfare, Kitakanamaru, Otawara, Tochigi, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tanabe
authors.forename: Eriko
authors.initials: E
authors.lastname: Fujita
authors.forename: Yukiko K
authors.initials: YK
authors.lastname: Hayashi
authors.forename: Xinran
authors.initials: X
authors.lastname: Zhu
authors.forename: Hermann
authors.initials: H
authors.lastname: Lubbert
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mezaki
authors.forename: Haruki
authors.initials: H
authors.lastname: Senoo
authors.forename: Takashi
authors.initials: T
authors.lastname: Momoi
mesh_terms: D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008565:Membrane Proteins; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism*; D054731:PDZ Domains; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
chemical_list: D002038:Bungarotoxins; C498598:Cadm1 protein, mouse; D002352:Carrier Proteins; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; C491750:Mpdz protein, mouse; D011993:Recombinant Fusion Proteins