title: Distinctive contributions of the ribosomal P-site elements m2G966, m5C967 and the C-terminal tail of the S9 protein in the fidelity of initiation of translation in Escherichia coli.
abstract: The accuracy of pairing of the anticodon of the initiator tRNA (tRNA(fMet)) and the initiation codon of an mRNA, in the ribosomal P-site, is crucial for determining the translational reading frame. However, a direct role of any ribosomal element(s) in scrutinizing this pairing is unknown. The P-site elements, m(2)G966 (methylated by RsmD), m(5)C967 (methylated by RsmB) and the C-terminal tail of the protein S9 lie in the vicinity of tRNA(fMet). We investigated the role of these elements in initiation from various codons, namely, AUG, GUG, UUG, CUG, AUA, AUU, AUC and ACG with tRNA(fMet(CAU) (tRNA(fMet) with CAU anticodon); CAC and CAU with tRNA(fMet(GUG); UAG with tRNA(fMet(CAU) ; UAC with tRNA(fMet(GUG) ; and AUC with tRNA(fMet(GUG) using in vivo and computational methods. Although RsmB deficiency did not impact initiation from most codons, RsmD deficiency increased initiation from AUA, CAC and CAU (2- to 3.6-fold). Deletion of the S9 C-terminal tail resulted in poorer initiation from UUG, GUG and CUG, but in increased initiation from CAC, CAU and UAC codons (up to 4-fold). Also, the S9 tail suppressed initiation with tRNA(fMet(CAU) lacking the 3GC base pairs in the anticodon stem. These observations suggest distinctive roles of 966/967 methylations and the S9 tail in initiation.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Smriti
authors.initials: S
authors.lastname: Arora
authors.forename: Satya Prathyusha
authors.initials: SP
authors.lastname: Bhamidimarri
authors.forename: Moitrayee
authors.initials: M
authors.lastname: Bhattacharyya
authors.forename: Ashwin
authors.initials: A
authors.lastname: Govindan
authors.forename: Michael H W
authors.initials: MH
authors.lastname: Weber
authors.forename: Saraswathi
authors.initials: S
authors.lastname: Vishveshwara
authors.forename: Umesh
authors.initials: U
authors.lastname: Varshney
mesh_terms: D000926:Anticodon; D003062:Codon; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D008745:Methylation; D056004:Molecular Dynamics Simulation; D009154:Mutation; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics; D012270:Ribosomes / Q000737:chemistry*; D017384:Sequence Deletion
chemical_list: D000926:Anticodon; D003062:Codon; D029968:Escherichia coli Proteins; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; D012358:RNA, Transfer, Met; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; C024988:tRNA, formylmethionine-