title: Burkholderia cepacia complex infection in an Adult Cystic Fibrosis unit in Madrid.
abstract: BACKGROUND
  Burkholderia cepacia complex have emerged as significant pathogens in cystic fibrosis (CF) patients due to the risk of cepacia syndrome and the innate multi-resistance of the microorganisms to antibiotics. The aim of this study was to describe the antimicrobial susceptibility profiles, the genotypes and subtypes of BCC, and the clinical evolution of CF patients with BCC.
  
  
  METHODS
  The lung function and Brasfield and Shwachman score were assessed in 12 patients. BCC were identified and susceptibility was studied by MicroScan (Siemens). Species and genospecies of BCC were confirmed by molecular methods in a Reference Centre (Majadahonda).
  
  
  RESULTS
  BCC were identified in 12 of 70 patients (17.1%) over a ten year period. The mean age to colonization by BCC was 24.4 years (SD: 7.71). B. cenocepacia was isolated in 4 patients (33.3%), B. contaminans was isolated in 3 patients (25%), both B. vietnamiensis and B. stabilis were isolated in 2 patients (16.7%), and B. cepacia, B. multivorans and B. late were isolated in one patient (8.3%). Among the B. cenocepacia, subtype IIIa was identified in two strains, and subtype IIIb was identified in the other two strains. There was susceptibility to meropenem in 90% of BCC, 80% to cotrimoxazole, 60% to minocycline, 50% to ceftazidime, and 40% to levofloxacin.
  
  
  CONCLUSIONS
  B. cenocepacia was the most prevalent species among the BCC isolated in CF adult patients, and subtypes IIIa and IIIb were identified in the 50% of the strains. Meropenem and cotrimoxazole showed the best activity.
authors.affiliation: Servicio de Microbiología, Instituto de Investigación Sanitaria, Hospital de La Princesa, Madrid, Spain. Electronic address: ana-correa@hotmail.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Correa-Ruiz
authors.forename: Rosa
authors.initials: R
authors.lastname: Girón
authors.forename: Buenaventura
authors.initials: B
authors.lastname: Buendía
authors.forename: M José
authors.initials: MJ
authors.lastname: Medina-Pascual
authors.forename: Claudia
authors.initials: C
authors.lastname: Valenzuela
authors.forename: Manuel
authors.initials: M
authors.lastname: López-Brea
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Sáez-Nieto
mesh_terms: D000328:Adult; D019121:Burkholderia Infections / Q000150:complications / Q000188:drug therapy; D042602:Burkholderia cepacia complex / Q000145:classification / Q000187:drug effects; D003550:Cystic Fibrosis / Q000150:complications; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D013030:Spain; D055815:Young Adult
keywords: Burkholderia cepacia complex; Cystic fibrosis; Fibrosis quística; Función pulmonar; Lung function; Molecular methods; Métodos moleculares