title: Native adipose stromal cells egress from adipose tissue in vivo: evidence during lymph node activation.
abstract: Adipose tissue (AT) has become accepted as a source of multipotent progenitor cells, the adipose stromal cells (ASCs). In this regard, considerable work has been performed to harvest and characterize this cell population as well as to investigate the mechanisms by which transplanted ASCs mediate tissue regeneration. In contrast the endogenous release of native ASCs by AT has been poorly investigated. In this work, we show that native ASCs egress from murine AT. Indeed, we demonstrated that the release of native ASCs from AT can be evidenced both using an ex vivo perfusion model that we set up and in vivo. Such a mobilization process is controlled by CXCR4 chemokine receptor. In addition, once mobilized from AT, circulating ASCs were found to navigate through lymph fluid and to home into lymph nodes (LN). Therefore, we demonstrated that, during the LN activation, the fat depot encapsulating the activated LN releases native ASCs, which in turn invade the activated LN. Moreover, the ASCs invading the LN were visualized in close physical interaction with podoplanin and ER-TR7 positive structures corresponding to the stromal network composing the LN. This dynamic was impaired with CXCR4 neutralizing antibody. Taken together, these data provide robust evidences that native ASCs can traffic in vivo and that AT might provide stromal cells to activated LNs.
authors.affiliation: CNRS, Université Toulouse III, Toulouse, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Gil-Ortega
authors.forename: Lucile
authors.initials: L
authors.lastname: Garidou
authors.forename: Corinne
authors.initials: C
authors.lastname: Barreau
authors.forename: Marie
authors.initials: M
authors.lastname: Maumus
authors.forename: Ludovic
authors.initials: L
authors.lastname: Breasson
authors.forename: Geneviève
authors.initials: G
authors.lastname: Tavernier
authors.forename: Concha F
authors.initials: CF
authors.lastname: García-Prieto
authors.forename: Anne
authors.initials: A
authors.lastname: Bouloumié
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.forename: Coralie
authors.initials: C
authors.lastname: Sengenès
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D054377:Chemokine CXCL12 / Q000378:metabolism; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000166:cytology* / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019718:Receptors, CXCR4 / Q000378:metabolism
chemical_list: C514936:CXCR4 protein, mouse; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D019718:Receptors, CXCR4
keywords: Chemotaxis; Immunization; Lymph node remodeling; Mesenchymal stem cells; Mobilization