title: Irradiation induced injury reduces energy metabolism in small intestine of Tibet minipigs.
abstract: BACKGROUND
  The radiation-induced energy metabolism dysfunction related to injury and radiation doses is largely elusive. The purpose of this study is to investigate the early response of energy metabolism in small intestinal tissue and its correlation with pathologic lesion after total body X-ray irradiation (TBI) in Tibet minipigs.
  
  
  RESULTS
  30 Tibet minipigs were assigned into 6 groups including 5 experimental groups and one control group with 6 animals each group. The minipigs in these experimental groups were subjected to a TBI of 2, 5, 8, 11, and 14 Gy, respectively. Small intestine tissues were collected at 24 h following X-ray exposure and analyzed by histology and high performance liquid chromatography (HPLC). DNA contents in this tissue were also examined. Irradiation causes pathologic lesions and mitochondrial abnormalities. The Deoxyribonucleic acid (DNA) content-corrected and uncorrected adenosine-triphosphate (ATP) and total adenine nucleotides (TAN) were significantly reduced in a dose-dependent manner by 2-8 Gy exposure, and no further reduction was observed over 8 Gy.
  
  
  CONCLUSIONS
  TBI induced injury is highly dependent on the irradiation dosage in small intestine and inversely correlates with the energy metabolism, with its reduction potentially indicating the severity of injury.
authors.affiliation: Department of Laboratory Animal Center, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Yu-Jue
authors.initials: YJ
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.forename: Chi
authors.initials: C
authors.lastname: Chen
authors.forename: Li-Meng
authors.initials: LM
authors.lastname: Yan
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.forename: Kun-Yuan
authors.initials: KY
authors.lastname: Guo
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Wu
authors.forename: Wei-Wang
authors.initials: WW
authors.lastname: Gu
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D004249:DNA Damage / Q000528:radiation effects; D004734:Energy Metabolism / Q000528:radiation effects*; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000378:metabolism*; D013552:Swine; D013556:Swine, Miniature / Q000378:metabolism*; D013997:Time Factors; D014216:Triacetoneamine-N-Oxyl / Q000378:metabolism; D014916:Whole-Body Irradiation
chemical_list: D000227:Adenine Nucleotides; D014216:Triacetoneamine-N-Oxyl