title: [Purification of arsenic-binding proteins in hamster plasma after oral administration of arsenite].
abstract: To purify the arsenic-binding proteins (As-BP) in hamster plasma after a single oral administration of arsenite (iAs(III)).
  
  
  
  Arsenite was given to hamsters in a single dose. Three types of HPLC columns, size exclusion, gel filtration and anion exchange columns, combined with an inductively coupled argon plasma mass spectrometer (ICP MS) were used to purify the As-BP in hamster plasma. SDS-PAGE was used to confirm the arsenic-binding proteins at each purification step.
  
  
  
  The three-step purification process successfully separated As-BP from other proteins (ie, arsenic unbound proteins) in hamster plasma. The molecular mass of purified As-BP in plasma was approximately 40-50 kD on SDS-PAGE.
  
  
  
  The three-step purification method is a simple and fast approach to purify the As-BP in plasma samples.
authors.affiliation: Chengde Medical College, Chengde, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Chunhui
authors.initials: C
authors.lastname: Li
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Qin
authors.forename: Naranmandura
authors.initials: N
authors.lastname: Hua
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001151:Arsenic / Q000097:blood*; D018053:Arsenites / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002352:Carrier Proteins / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006224:Cricetinae
chemical_list: D018053:Arsenites; D002352:Carrier Proteins; C015001:arsenite; D001151:Arsenic