title: Selective serial multi-antibody biosensing with TOPAS microstructured polymer optical fibers.
abstract: We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.
authors.affiliation: DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark. egrigoriy@hotmail.com
authors.forename: Grigoriy
authors.initials: G
authors.lastname: Emiliyanov
authors.forename: Poul E
authors.initials: PE
authors.lastname: Høiby
authors.forename: Lars H
authors.initials: LH
authors.lastname: Pedersen
authors.forename: Ole
authors.initials: O
authors.lastname: Bang
mesh_terms: D000906:Antibodies / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D055100:Optical Fibers; D011108:Polymers / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000906:Antibodies; D002232:Carbocyanines; D011108:Polymers; C089187:cyanine dye 3; C085321:cyanine dye 5; D019809:Streptavidin