title: An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules.
abstract: A bio-nanocapsule derived from the hepatitis B virus (HBV) is expected to be useful as a drug delivery system carrier. Because various types of bio-nanocapsules have been developed, a simple and versatile purification method for bio-nanocapsules would be useful. Therefore, this study was focused on establishing a simple purification method using affinity chromatography by inserting a histidine tag (His-tag) into a bio-nanocapsule. The method achieved a simple, one-step purification with a yield that was 2.5-fold higher than conventional ultracentrifugation, and thus would be a desirable alternative method for recombinant virus-like particle purification.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Koichi
authors.initials: K
authors.lastname: Takeda
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D001709:Biotechnology / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods*; D004337:Drug Carriers; D006515:Hepatitis B virus / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D053769:Nanocapsules; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D004337:Drug Carriers; D053769:Nanocapsules