title: Changes of DNA methylation and hydroxymethylation in plant protoplast cultures.
abstract: Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.
authors.affiliation: Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Moricová
authors.forename: Vladan
authors.initials: V
authors.lastname: Ondřej
authors.forename: Božena
authors.initials: B
authors.lastname: Navrátilová
authors.forename: Lenka
authors.initials: L
authors.lastname: Luhová
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D019175:DNA Methylation; D010944:Plants / Q000737:chemistry*; D011523:Protoplasts / Q000737:chemistry*; D017382:Reactive Oxygen Species
chemical_list: D017382:Reactive Oxygen Species