title: Methanogenic community dynamics in anaerobic co-digestion of fruit and vegetable waste and food waste.
abstract: A lab-scale continuously-stirred tank reactor (CSTR), used for anaerobic co-digestion of fruit and vegetable waste (FVW) and food waste (FW) at different mixture ratios, was operated for 178 days at the organic loading rate of 3 kg VS (volatile solids)/(m3 x day). The dynamics of the Archaeal community and the correlations between environmental variables and methanogenic community structure were analyzed by polymerase chain reactions--denaturing gradient gel electrophoresis (PCR-DGGE) and redundancy analysis (RDA), respectively. PCR-DGGE results demonstrated that the mixture ratio of FVW to FW altered the community composition of Archaea. As the FVW/FW ratio increased, Methanoculleus, Methanosaeta and Methanosarcina became the predominant methanogens in the community. Redundancy analysis results indicated that the shift of the methanogenic community was significantly correlated with the composition of acidogenic products and methane production yield. Different mixture ratios of substrates led to different compositions of intermediate metabolites, which may affect the methanogenic community. These results suggested that the analysis of microbial communities could be used to diagnose anaerobic processes.
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control (SKLESPC), School ofEnvironment, Tsinghua University, Beijing 100084, China. tsinghuaenv@gmail.com
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
authors.forename: Jiane
authors.initials: J
authors.lastname: Zuo
authors.forename: Ruofan
authors.initials: R
authors.lastname: Ji
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Chen
authors.forename: Fenglin
authors.initials: F
authors.lastname: Liu
authors.forename: Kaijun
authors.initials: K
authors.lastname: Wang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D058645:Denaturing Gradient Gel Electrophoresis; D005735:Garbage; D008697:Methane / Q000378:metabolism*; D059013:Microbial Consortia; D015999:Multivariate Analysis; D016133:Polymerase Chain Reaction
chemical_list: D008697:Methane