title: Highly efficient förster resonance energy transfer in a fast, serum-compatible immunoassay.
abstract: Highly efficient FRET leads to important enhancements for homogeneous immunoassays. By using the novel phosphorescent dye EuLH and BHQ-10 as a donor-acceptor pair, the FRET efficiency increases to >99.5 %, leading to significantly improved signal-to-background ratio, precision and linear range. The phosphorescence detection enabled full compatibility to serum samples for this fast-responding immunoassay.
authors.affiliation: Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kreisig
authors.forename: Ralf
authors.initials: R
authors.lastname: Hoffmann
authors.forename: Thole
authors.initials: T
authors.lastname: Zuchner
mesh_terms: D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000191:economics / Q000379:methods*; D007118:Immunoassay / Q000191:economics / Q000379:methods; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D044967:Serum / Q000737:chemistry*; D013552:Swine
chemical_list: D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D010455:Peptides