title: Cleaved caspase-3 expression in hypothalamic magnocellular neurons may affect vasopressin secretion during experimental polymicrobial sepsis.
abstract: We investigated whether the vasopressin (AVP) secretion deficiency observed during cecal ligation and puncture (CLP)-induced sepsis may be caused by apoptosis in hypothalamic magnocellular neurons. Plasma cytokines (TNF-α, IL-1β and IL-6) and nitrate levels were increased during sepsis and plasma AVP levels were higher in the early phase returning to basal levels in the late phase. Concomitantly, expression of the apoptosis effector, cleaved caspase 3, was increased in magnocellular neurons, inferring that this increase in hypothalamic neurons may be caused by cytokines and elevated nitrate levels. This in turn could compromise AVP secretion in the late phase of sepsis.
authors.affiliation: Departamento de Morfologia, Estomatologia e Fisiologia, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Gabriela Ravanelli
authors.initials: GR
authors.lastname: Oliveira-Pelegrin
authors.forename: Paulo José
authors.initials: PJ
authors.lastname: Basso
authors.forename: Aline Souza
authors.initials: AS
authors.lastname: Soares
authors.forename: Mariana Rossin
authors.initials: MR
authors.lastname: Martinez
authors.forename: Karin Dorota
authors.initials: KD
authors.lastname: Riester
authors.forename: Maria José Alves
authors.initials: MJ
authors.lastname: Rocha
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009566:Nitrates / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018805:Sepsis / Q000378:metabolism*; D014667:Vasopressins / Q000378:metabolism*
chemical_list: D016207:Cytokines; D009566:Nitrates; D014667:Vasopressins; D053148:Caspase 3