title: Determination of burn patient outcome by large-scale quantitative discovery proteomics.
abstract: OBJECTIVE
  Emerging proteomics techniques can be used to establish proteomic outcome signatures and to identify candidate biomarkers for survival following traumatic injury. We applied high-resolution liquid chromatography-mass spectrometry and multiplex cytokine analysis to profile the plasma proteome of survivors and nonsurvivors of massive burn injury to determine the proteomic survival signature following a major burn injury.
  
  
  METHODS
  Proteomic discovery study.
  
  
  METHODS
  Five burn hospitals across the United States.
  
  
  METHODS
  Thirty-two burn patients (16 nonsurvivors and 16 survivors), 19-89 years old, were admitted within 96 hours of injury to the participating hospitals with burns covering more than 20% of the total body surface area and required at least one surgical intervention.
  
  
  METHODS
  None.
  
  
  RESULTS
  We found differences in circulating levels of 43 proteins involved in the acute-phase response, hepatic signaling, the complement cascade, inflammation, and insulin resistance. Thirty-two of the proteins identified were not previously known to play a role in the response to burn. Interleukin-4, interleukin-8, granulocyte macrophage colony-stimulating factor, monocyte chemotactic protein-1, and β2-microglobulin correlated well with survival and may serve as clinical biomarkers.
  
  
  CONCLUSIONS
  These results demonstrate the utility of these techniques for establishing proteomic survival signatures and for use as a discovery tool to identify candidate biomarkers for survival. This is the first clinical application of a high-throughput, large-scale liquid chromatography-mass spectrometry-based quantitative plasma proteomic approach for biomarker discovery for the prediction of patient outcome following burn, trauma, or critical illness.
authors.affiliation: Department of Surgery, University of Texas Medical Branch, and Shriners Hospitals for Children, Galveston, TX, USA. ccfinner@utmb.edu
authors.forename: Celeste C
authors.initials: CC
authors.lastname: Finnerty
authors.forename: Marc G
authors.initials: MG
authors.lastname: Jeschke
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Qian
authors.forename: Amit
authors.initials: A
authors.lastname: Kaushal
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Xiao
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Marina A
authors.initials: MA
authors.lastname: Gritsenko
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.forename: David G
authors.initials: DG
authors.lastname: Camp
authors.forename: Lyle L
authors.initials: LL
authors.lastname: Moldawer
authors.forename: Constance
authors.initials: C
authors.lastname: Elson
authors.forename: David
authors.initials: D
authors.lastname: Schoenfeld
authors.forename: Richard
authors.initials: R
authors.lastname: Gamelli
authors.forename: Nicole
authors.initials: N
authors.lastname: Gibran
authors.forename: Matthew
authors.initials: M
authors.lastname: Klein
authors.forename: Brett
authors.initials: B
authors.lastname: Arnoldo
authors.forename: Daniel
authors.initials: D
authors.lastname: Remick
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Ronald
authors.initials: R
authors.lastname: Davis
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: David N
authors.initials: DN
authors.lastname: Herndon
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D002053:Burn Units; D002056:Burns / Q000097:blood* / Q000175:diagnosis / Q000401:mortality*; D016207:Cytokines / Q000097:blood* / Q000378:metabolism; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011379:Prognosis; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D016207:Cytokines; D018836:Inflammation Mediators; D020543:Proteome