title: Isolation of high-quality total RNA from lipid-rich seeds.
abstract: Obtaining high-quality RNA from lipid-rich seeds has been a challenge, since lipids can interfere with tissue disruption and block cells from extraction buffer, and polysaccharides, polyphenols, and other secondary metabolites can bind or coprecipitate with RNA. Using an improved CTAB-based extraction buffer and modified grinding and incubation method, we developed a protocol that is able to minimize the negative effects caused by lipids and other compounds. Using this protocol, we obtained high-quality and high-quantity RNA from six species of lipid-rich seeds within 3 hours. The isolated RNA was demonstrated to be suitable for downstream applications.
authors.affiliation: Department of Biology, Chongqing University of Science and Technology, Chongqing 401331, People's Republic of China. tianying@buffalo.edu
authors.forename: Tianying
authors.initials: T
authors.lastname: Lan
authors.forename: Bo
authors.initials: B
authors.lastname: Yao
authors.forename: Ye
authors.initials: Y
authors.lastname: Shen
authors.forename: Xi'an
authors.initials: X
authors.lastname: Wang
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D050356:Lipid Metabolism; D018749:RNA, Plant / Q000302:isolation & purification*; D031287:Sapium / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*
chemical_list: D002593:Cetrimonium Compounds; D018749:RNA, Plant; D000077286:Cetrimonium