title: Involvement of nuclear factor of activated T cells in granulocyte-macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease.
abstract: BACKGROUND
  A previous study demonstrated that the cysteine protease of Dermatophagoides farinae induced production of granulocyte-macrophage colony-stimulating factor (GM-CSF) in a canine epidermal keratinocyte progenitor cell line (CPEK); however, the molecular mechanism has not been elucidated.
  
  
  OBJECTIVE
  Given that the transcription of GM-CSF mRNA in human lymphocytes is mainly regulated by the nuclear factor of activated T cells (NFAT), it is hypothesized that NFAT also contributes to GM-CSF production in canine keratinocytes stimulated with a cysteine protease.
  
  
  METHODS
  Nuclear translocation of NFAT was evaluated in CPEK cells in the absence or presence of the cysteine protease papain. We also investigated whether blockade of NFAT could inhibit GM-CSF production.
  
  
  RESULTS
  Papain-induced nuclear translocation of NFAT, producing GM-CSF, was partly inhibited by ciclosporin.
  
  
  CONCLUSIONS
  The results suggest that GM-CSF production mediated by the cysteine protease is regulated not only by NFAT but also by unknown signalling pathways in canine keratinocytes.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.forename: Machiko
authors.initials: M
authors.lastname: Sekido
authors.forename: Aki
authors.initials: A
authors.lastname: Iio
authors.forename: Naoki
authors.initials: N
authors.lastname: Chimura
authors.forename: Sanae
authors.initials: S
authors.lastname: Shibata
authors.forename: Harumi
authors.initials: H
authors.lastname: Kamishina
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
mesh_terms: D000818:Animals; D002460:Cell Line; D016572:Cyclosporine / Q000494:pharmacology; D015853:Cysteine Proteinase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism*; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010206:Papain / Q000008:administration & dosage / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism
chemical_list: D015853:Cysteine Proteinase Inhibitors; D050778:NFATC Transcription Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016572:Cyclosporine; D010206:Papain