title: LC-MS/MS determination of pogostone in rat plasma and its application in pharmacokinetic studies.
abstract: Pogostone is an important constituent of Pogostemon cablin (Blanco) Benth., and possesses various known bioactivities. A rapid, simple and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the analysis of pogostone in rat plasma using chrysophanol as internal standard (IS). The analytes were extracted with methanol and separated using a reversed-phase YMC-UltraHT Pro C18 column. Elution was achieved with a mobile phase consisting of methanol-water (75:25, v/v) for 5 min at a flow rate of 400 μL/min. The precursor/product transitions (m/z) under MS/MS detection with negative electrospray ionization (ESI) were 223.0 → 139.0 and 253.1 → 224.9 for pogostone and IS, respectively. The calibration curve was linear over the concentration range 0.05-160 µg/mL (r = 0.9996). The intra- and inter-day accuracy and precision were within ±10%. The validated method was successfully applied to the preclinical pharmacokinetic investigation of pogostone in rats after intravenous (5, 10 and 20 mg/kg) and oral administration (5, 10 and 20 mg/kg). Finally, the oral absolute bioavailability of pogostone in rats was calculated to be 70.39, 78.18 and 83.99% for 5, 10 and 20 mg/kg, respectively.
authors.affiliation: College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou, PR China.
authors.forename: Haiming
authors.initials: H
authors.lastname: Chen
authors.forename: Yucui
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.forename: Chuwen
authors.initials: C
authors.lastname: Li
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qin
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Yi
authors.forename: Jiannan
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lai
authors.forename: Ziren
authors.initials: Z
authors.lastname: Su
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000097:blood; D001774:Blood Chemical Analysis; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000880:Anthraquinones; D009822:Oils, Volatile; C483658:Pogostone; C027113:chrysophanic acid
keywords: LC-MS/MS; pharmacokinetics; pogostone; rat plasma