title: Molecular characterization of a new alphasatellite associated with a cassava mosaic geminivirus in Madagascar.
abstract: Two complete nucleotide sequences of an alphasatellite isolated from a cassava plant with mosaic disease symptoms in Madagascar are described and analyzed. While the helper begomovirus was identified as an isolate of East African cassava mosaic Kenya virus (EACMKV), its associated alphasatellite was most closely related (80 % nucleotide sequence identity) to cotton leaf curl Gezira alphasatellite. These satellite molecules have typical features of alphasatellites, with a single gene in the virion sense, an A-rich region and a stem-loop structure. According to the proposed species demarcation threshold of alphasatellites (83 % nucleotide identity), they are isolates of a new species for which we propose the name "Cassava mosaic alphasatellite".
authors.affiliation: CIRAD, UMR PVBMT, Pôle de Protection des Plantes, 7 Chemin de l'IRAT, 97410, Saint-Pierre, Ile de La Réunion, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Harimalala
authors.forename: Alexandre
authors.initials: A
authors.lastname: De Bruyn
authors.forename: Murielle
authors.initials: M
authors.lastname: Hoareau
authors.forename: Alice
authors.initials: A
authors.lastname: Andrianjaka
authors.forename: Sahondramalala
authors.initials: S
authors.lastname: Ranomenjanahary
authors.forename: Bernard
authors.initials: B
authors.lastname: Reynaud
authors.forename: Pierre
authors.initials: P
authors.lastname: Lefeuvre
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Lett
mesh_terms: D053839:Begomovirus / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics* / Q000302:isolation & purification; D008270:Madagascar; D002365:Manihot / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004276:DNA, Satellite