title: Histopathological evaluation of the efficacy of antifungals for experimental Trichosporon bloodstream infection.
abstract: The efficacy of polyene macrolides to treat experimental Trichosporon bloodstream infection was evaluated by histopathological examination and viable cell counts in the kidneys of infected mice. Viable cell counts on the 5th day after infection confirmed that liposomal amphotericin B (L-AMB) is a more effective treatment than fluconazole (FLC) for mice infected with an azole-resistant strain of Trichosporon. Histological examination revealed that the administration of L-AMB induced a transformation from acute purulent inflammation caused by both azole-susceptible and -resistant strain infections to a chronic and subsiding form, whereas FLC failed to convert the acute inflammation induced by the azole-resistant strain to a subsiding form. Our results demonstrate that polyene macrolides can be used as an alternative therapy for infection of azole-resistant strains of Trichosporon and that histopathological evaluation is useful for elucidating the pathophysiology of an experimental Trichosporon infection.
authors.affiliation: Department of Surgical Pathology, Toho University Omori Medical Center, Tokyo 143-8541, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sasai
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Okubo
authors.forename: Takao
authors.initials: T
authors.lastname: Ishiwatari
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugita
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kaneko
authors.forename: Somay Yamagata
authors.initials: SY
authors.lastname: Murayama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shimamura
authors.forename: Minoru
authors.initials: M
authors.lastname: Shinozaki
authors.forename: Chikako
authors.initials: C
authors.lastname: Hasegawa
authors.forename: Aki
authors.initials: A
authors.lastname: Mitsuda
authors.forename: Naobumi
authors.initials: N
authors.lastname: Tochigi
authors.forename: Megumi
authors.initials: M
authors.lastname: Wakayama
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nemoto
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shibuya
mesh_terms: D000666:Amphotericin B / Q000627:therapeutic use; D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D015725:Fluconazole / Q000627:therapeutic use; D016469:Fungemia / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology*; D006651:Histocytochemistry; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D018942:Macrolides / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D011090:Polyenes / Q000627:therapeutic use; D014250:Trichosporon / Q000187:drug effects*; D060586:Trichosporonosis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology*
chemical_list: D000935:Antifungal Agents; D018942:Macrolides; D011090:Polyenes; D000666:Amphotericin B; D015725:Fluconazole