title: Gluten-sensitive enteropathy coincides with decreased capability of intestinal T cells to secrete IL-17 and IL-22 in a macaque model for celiac disease.
abstract: Celiac disease (CD) is an autoimmune disorder caused by intolerance to dietary gluten. The interleukin (IL)-17 and IL-22 function as innate regulators of mucosal integrity. Impaired but not well-understood kinetics of the IL-17/22 secretion was described in celiac patients. Here, the IL-17 and IL-22-producing intestinal cells were studied upon their in vitro stimulation with mitogens in class II major histocompatibility complex-defined, gluten-sensitive rhesus macaques. Pediatric biopsies were collected from distal duodenum during the stages of disease remission and relapse. Regardless of dietary gluten content, IL-17 and IL-22-producing cells consisted of CD4+ and CD8+ T lymphocytes as well as of lineage-negative (Lin-) cells. Upon introduction of dietary gluten, capability of intestinal T cells to secrete IL-17/22 started to decline (p<0.05), which was paralleled with gradual disruption of epithelial integrity. These data indicate that IL-17/22-producing cells play an important role in maintenance of intestinal mucosa in gluten-sensitive primates.
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Huanbin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Feely
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: David X
authors.initials: DX
authors.lastname: Liu
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Juan T
authors.initials: JT
authors.lastname: Borda
authors.affiliation: Division of Veterinary Resources, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Dufour
authors.affiliation: Department of Chemistry and Biochemistry, Stanford University, Stanford, CA, USA.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Li
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA; Division of Veterinary Resources, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Pyone P
authors.initials: PP
authors.lastname: Aye
authors.affiliation: Comparative Genetics and Refinement, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors.forename: Gaby G
authors.initials: GG
authors.lastname: Doxiadis
authors.affiliation: Department of Chemistry and Biochemistry, Stanford University, Stanford, CA, USA.
authors.forename: Chaitan
authors.initials: C
authors.lastname: Khosla
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Veazey
authors.affiliation: Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA. Electronic address: ksestak@tulane.edu.
authors.forename: Karol
authors.initials: K
authors.lastname: Sestak
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002446:Celiac Disease / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D004386:Duodenum / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D005983:Glutens / Q000276:immunology; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology* / Q000378:metabolism; D007378:Interleukins / Q000276:immunology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007422:Intestines / Q000276:immunology* / Q000473:pathology; D018655:Lymphocyte Count; D008253:Macaca mulatta; D018613:Microscopy, Confocal; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D005983:Glutens; D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22