title: Alterations in mitochondrial membrane in chemopreventive action of fish oil.
abstract: Mitochondria are major regulators of pathways related to tumorigenesis; therefore, mitochondrial membrane characteristics and associated cell signaling events were evaluated with different ratios of fish oil (FO) and corn oil (CO) in experimental colon carcinogenesis. Treatment with carcinogen 1,2-dimethylhydrazine (DMH) altered reactive oxygen species (ROS), Ca(2+), and membrane characteristics, which resulted in an elevation in apoptosis in initiation phase and reduction in post-initiation phase. FO+CO(2.5:1)+DMH treatment, however, altered mitochondrial membrane parameters, ROS, and Ca(2+) to increase apoptosis in both phases, whereas FO+CO(1:1)+DMH treatment enhanced apoptosis only in post-initiation phase suggesting that FO supplementation in higher ratio has better chemopreventive efficacy.
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh, India.
authors.forename: Gayatri
authors.initials: G
authors.lastname: Sharma
authors.forename: Isha
authors.initials: I
authors.lastname: Rani
authors.forename: Shevali
authors.initials: S
authors.lastname: Kansal
authors.forename: Archana
authors.initials: A
authors.lastname: Bhatnagar
authors.forename: Navneet
authors.initials: N
authors.lastname: Agnihotri
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000494:pharmacology; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000378:metabolism; D018890:Chemoprevention / Q000379:methods; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism / Q000517:prevention & control*; D003314:Corn Oil / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D005395:Fish Oils; D017382:Reactive Oxygen Species; D003314:Corn Oil; D053148:Caspase 3; D019813:1,2-Dimethylhydrazine; D002118:Calcium