title: Chitosan nanoparticle carrying small interfering RNA to platelet-derived growth factor B mRNA inhibits proliferation of smooth muscle cells in rabbit injured arteries.
abstract: The purpose of this study was to elucidate the transfection of chitosan nanoparticle carrying small interfering RNA against platelet-derived growth factor B (PDGF-B) to inhibit the expression of PDGF-B mRNA and proliferation of smooth muscle cells. A rabbit iliac artery injury model was constructed. A small interfering RNA (siRNA) against PDGF-B mRNA expression vector was constructed and packaged by chitosan nanoparticle to transfect into the vascular smooth muscle cells (vSMCs) of balloon catheter-injured rabbit iliac artery wall, using a therapeutic ultrasound for the gene delivery. The experiment was divided into two groups: experimental group, denudation and nano-PDGF-B siRNA treated, and only single denudation as control. Effects of the siRNA on the expressions of proliferating cell nuclear antigen (PCNA) and PDGF-B mRNA by vSMCs and the proliferation of vSMCs were observed with the methods of routine pathological, immunohistochemical staining, in situ hybridization and morphometry. The nano siRNA against PDGF-B was successfully transfected. The nano siRNA significantly inhibited the expressions of PCNA and PDGF-B mRNA in intimal vSMCs. The local intimal thickness and area were also reduced remarkably. In conclusion, transfection of chitosan nanoparticle carrying siRNA against PDGF-B mRNA could inhibit proliferation of vSMCs in the rabbit iliac artery injury model.
authors.affiliation: The Department of Pathology, Shenzhen Sun Yet-Sen Cardiovascular Hospital, Shenzhen 518020, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Xia
authors.affiliation: The Department of Pathology, Shenzhen Sun Yet-Sen Cardiovascular Hospital, Shenzhen 518020, PR China jijun3@126.com.
authors.forename: Ji
authors.initials: J
authors.lastname: Jun
authors.affiliation: The Department of Pathology, Shenzhen Sun Yet-Sen Cardiovascular Hospital, Shenzhen 518020, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wen-Ping
authors.affiliation: National Hepatobiliary and Enteric Surgery Research Center, Central South University, Changsha 410008, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Yi-Feng
authors.affiliation: The Department of Pathology, Shenzhen Sun Yet-Sen Cardiovascular Hospital, Shenzhen 518020, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xiao-Ling
mesh_terms: D000800:Angioplasty, Balloon; D000818:Animals; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D004195:Disease Models, Animal; D007083:Iliac Artery / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000293:injuries / Q000378:metabolism / Q000473:pathology*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology*; D053758:Nanoparticles; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000067492:RNAi Therapeutics / Q000379:methods*; D011817:Rabbits; D014162:Transfection / Q000379:methods*; D014465:Ultrasonics; D057772:Vascular System Injuries / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D020574:Proto-Oncogene Proteins c-sis; D034741:RNA, Small Interfering; D048271:Chitosan
keywords: platelet-derived growth factor B; small interfering RNA; smooth muscle cell