title: Optimization on preparation condition of propolis flavonoids liposome by response surface methodology and research of its immunoenhancement activity.
abstract: The aim of this study is to prepare propolis flavonoids liposome (PFL) and optimize the preparation condition and to investigate further whether liposome could promote the immunoenhancement activity of propolis flavonoids (PF). PFL was prepared with ethanol injection method, and the preparation conditions of PFL were optimized with response surface methodology (RSM). Moreover, the immunoenhancement activity of PFL and PF in vitro was determined. The result showed that the optimal preparation conditions for PFL by response surface methodology were as follows: ratio of lipid to drug (w/w) 9.6 : 1, ratio of soybean phospholipid to cholesterol (w/w) 8.5 : 1, and speed of injection 0.8 mL·min(-1). Under these conditions, the experimental encapsulation efficiency of PFL was 91.67 ± 0.21%, which was close to the predicted value. Therefore, the optimized preparation condition is very reliable. Moreover, the results indicated that PFL could not only significantly promote lymphocytes proliferation singly or synergistically with PHA, but also increase expression level of IL-2 and IFN-γ mRNA. These indicated that liposome could significantly improve the immunoenhancement activity of PF. PFL demonstrates the significant immunoenhancement activity, which provides the theoretical basis for the further experiment in vivo.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Yuan
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.forename: Saifuding
authors.initials: S
authors.lastname: Abula
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Fan
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhao
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Cui
authors.initials: C
authors.lastname: Liu