title: Activity of corilagin on post-parasiticide liver fibrosis in Schistosomiasis animal model.
abstract: This study investigates the effects and possible molecular mechanisms of corilagin extraction on prevention of Schistosoma japonicum ova-induced granulomas and liver fibrosis. As a result, under a light microscope, when compared to a model group, the corilagin group showed smaller granulomas, less liver cell denaturation and less inflammatory cell infiltration, and the connective tissues were significantly decreased. By Masson staining, the liver sections from the corilagin group showed less collagen distributed around granulomas, decreased liver fibrosis in the portal tracts and less formed interlobular tissue. The expression of hydroxyproline, IL-13 in liver and GATA3 in spleen in the model group was significantly higher than that in the normal group (P less than 0.05 or 0.01), while the level of hydroxyproline, IL-13 and GATA3 in the corilagin group were significantly lower than that in the model group (P less than 0.05). In conclusion, corilagin extraction can decrease the level of Th2-associated profibrotic cytokine IL-13, and down-regulate the transcription of GATA3 mRNA in spleen cells, which alleviate the hepatic fibrosis caused by egg granuloma in Schistosoma japonicum infection.
authors.affiliation: Department of Infectious Disease, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Huang
authors.forename: S-L
authors.initials: SL
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Jin
authors.forename: D
authors.initials: D
authors.lastname: Cheng
authors.forename: Y-P
authors.initials: YP
authors.lastname: Zhou
authors.forename: H-R
authors.initials: HR
authors.lastname: Li
authors.forename: Z-M
authors.initials: ZM
authors.lastname: Tang
authors.forename: J
authors.initials: J
authors.lastname: Xue
authors.forename: W
authors.initials: W
authors.lastname: Cai
authors.forename: J-H
authors.initials: JH
authors.lastname: Dong
authors.forename: L
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents; D004195:Disease Models, Animal; D050990:GATA3 Transcription Factor / Q000235:genetics; D005960:Glucosides / Q000494:pharmacology / Q000627:therapeutic use*; D006099:Granuloma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D047348:Hydrolyzable Tannins; D006909:Hydroxyproline / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D012554:Schistosomiasis japonica / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D000977:Antiparasitic Agents; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D005960:Glucosides; D047348:Hydrolyzable Tannins; D018793:Interleukin-13; D020011:Protective Agents; D012333:RNA, Messenger; C049096:corilagin; D006909:Hydroxyproline