title: Recording of ion channel activity in planar lipid bilayer experiments.
abstract: Planar lipid bilayer is an electrophysiological technique that enables study of functional activities of ion channels, porins, and other pore-forming molecular complexes. The main purpose of this method is to monitor ion channels' behavior at the single molecule level in the artificial membranes. Here, I describe the details of this technique that will underline formation of the lipid bilayers and incorporation and activation of the ion channel protein.
authors.affiliation: Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL, USA.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Zakharian
mesh_terms: D003584:Cytological Techniques / Q000379:methods*; D005898:Glass / Q000737:chemistry; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism*; D050053:TRPM Cation Channels / Q000378:metabolism; D013696:Temperature
chemical_list: D008051:Lipid Bilayers; D050053:TRPM Cation Channels; C425966:TRPM8 protein, human