title: Fluorescent polymeric micelles with aggregation-induced emission properties for monitoring the encapsulation of doxorubicin.
abstract: A new type of fluorescent polymeric micelles is developed by self-assembly from a series of amphiphilic block copolymers, poly(ethylene glycol)-b-poly[styrene-co-(2-(1,2,3,4,5-pentaphenyl-1H-silol-1-yloxy)ethyl methacrylate)] [PEG-b-P(S-co-PPSEMA)]. Their capability of loading doxorubicin (DOX) is investigated by monitoring the loading content, encapsulation efficiency, and photophysical properties of micelles. Förster resonance energy transfer from PPSEMA to DOX is observed in DOX-loaded micelles, which can serve as an indication of successful encapsulation of DOX in these micelles. The application of this new type of fluorescent polymeric micelles as a fluorescent probe and an anticancer drug carrier simultaneously is explored by studying the intracellular uptake of DOX-loaded micelles.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors.forename: Jen-Ing
authors.initials: JI
authors.lastname: Chen
authors.forename: Wen-Chung
authors.initials: WC
authors.lastname: Wu
mesh_terms: D000042:Absorption; D016923:Cell Death / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D004339:Drug Compounding / Q000379:methods*; D019073:HT29 Cells; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008823:Micelles; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence
chemical_list: D008823:Micelles; D011108:Polymers; D012996:Solutions; D011092:Polyethylene Glycols; D004317:Doxorubicin