title: Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel.
abstract: We fabricated a three-dimensional multilayered blood vessel model using human cells and high-strength PEG hydrogel. The hydrogel tube was physically suitable for perfusion culture, and cells were cultured on the hydrogel surface by binding with fibronectin. Using the layer-by-layer cell multilayered technique, we successfully constructed an artificial blood vessel.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Shinohara
authors.forename: Takanori
authors.initials: T
authors.lastname: Kihara
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Michiya
authors.initials: M
authors.lastname: Matsusaki
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Akashi
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
mesh_terms: D001808:Blood Vessels / Q000166:cytology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008953:Models, Anatomic; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D011092:Polyethylene Glycols / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D020100:Hydrogels; D011092:Polyethylene Glycols