title: Evaluation of the toxic and genotoxic potential of acid mine drainage using physicochemical parameters and bioassays.
abstract: Carboniferous activity generates acid mine drainage (AMD) which is capable of unleashing toxic effects on the exposed biota. The aim of this study was to evaluate the toxic and genotoxic potential of untreated-AMD and AMD treated with calcinated sediment, using physicochemical parameters and bioassays. Results revealed that untreated-AMD presented low pH values and elevated concentrations of the metals Fe, Al, Mn, Zn and Cu. High acute toxicity was observed in Artemia sp. and Daphnia magna, and sub-chronic toxicity and genotoxicity in Allium cepa L. as well as scission of plasmid DNA exposed to untreated-AMD. Treatment of AMD with calcinated sediment promoted the reduction of acidity and the removal of metals, as well as a reduction in toxic and genotoxic effects. In conclusion, the calcinated sediment can be used as an alternative AMD treatment.
authors.affiliation: LADEBIMA - Laboratório de Desenvolvimento de Biomateriais e Materiais Antimicrobianos, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Netto
authors.forename: R A
authors.initials: RA
authors.lastname: Madeira
authors.forename: F Z
authors.initials: FZ
authors.lastname: Silveira
authors.forename: M A
authors.initials: MA
authors.lastname: Fiori
authors.forename: E
authors.initials: E
authors.lastname: Angioleto
authors.forename: C T
authors.initials: CT
authors.lastname: Pich
authors.forename: R
authors.initials: R
authors.lastname: Geremias
mesh_terms: D000490:Allium / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D000818:Animals; D001156:Artemia / Q000187:drug effects; D001681:Biological Assay; D020552:Comet Assay; D004249:DNA Damage; D003621:Daphnia / Q000187:drug effects; D019015:Geologic Sediments / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000009:adverse effects* / Q000032:analysis; D007928:Lethal Dose 50; D008670:Metals / Q000032:analysis / Q000633:toxicity; D008906:Mining; D009153:Mutagens / Q000633:toxicity*; D010087:Oxides / Q000737:chemistry; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D010957:Plasmids; D018505:Waste Management / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
chemical_list: D007220:Industrial Waste; D008670:Metals; D009153:Mutagens; D010087:Oxides; D014874:Water Pollutants, Chemical