title: Next-generation sequencing technologies in diagnostic virology.
abstract: The data deluge produced by next-generation sequencing (NGS) technologies is an appealing feature for clinical virologists that are involved in the diagnosis of emerging viral infections, molecular epidemiology of viral pathogens, drug-resistance testing, and also like to do some basic and clinical research. Indeed, NGS platforms are being implemented in many clinical and research laboratories, as the costs of these platforms are progressively decreasing. We provide here some suggestions for virologists who are planning to implement a NGS platform in their clinical laboratory and an overview on the potential applications of these technologies in diagnostic virology.
authors.affiliation: Department of Molecular Medicine, University of Padova, Via A. Gabelli 63, I-35121 Padova, Italy. Electronic address: luisa.barzon@unipd.it.
authors.forename: Luisa
authors.initials: L
authors.lastname: Barzon
authors.forename: Enrico
authors.initials: E
authors.lastname: Lavezzo
authors.forename: Giulia
authors.initials: G
authors.lastname: Costanzi
authors.forename: Elisa
authors.initials: E
authors.lastname: Franchin
authors.forename: Stefano
authors.initials: S
authors.lastname: Toppo
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
mesh_terms: D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000639:trends; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000639:trends; D017720:Molecular Epidemiology / Q000379:methods; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology; D014780:Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
keywords: Bioinformatics; Deep/high throughput sequencing; EBV; Epstein-Barr virus; Genome sequencing; HBV; HCMV; HCV; HIV; HSV; NGS; Viral epidemiology; Viral metagenomices/viromics; Virus discovery; hepatitis B virus; hepatitis C virus; herpes simplex virus; human cytomegalovirus; human immunodeficiency virus; next generation sequencing