title: microRNA expression profiling in multidrug resistance of the 5‑Fu‑induced SGC‑7901 human gastric cancer cell line.
abstract: The present study aimed to identify microRNA (miRNA) expression profiles associated with multidrug resistance (MDR) in gastric carcinoma. A 5‑ﬂuorouracil (5‑Fu)‑induced MDR gastric cancer cell line was selected and miRNA expression profiling of the cell line was conducted following exposure to 5‑Fu. The miRNA expression profiles between the parental and resistant gastric cancer cells were analyzed by Human miRNA OneArray® v3 and the results were confirmed by quantitative real‑time RT‑PCR. The expression of 9 miRNAs (miR‑10b, ‑22, ‑31, ‑133b, ‑190, ‑501, ‑615, ‑501‑5p and ‑615‑5p) was upregulated while the expression of 18 additional miRNAs (miR‑32, ‑197, ‑210, ‑766, ‑1229, ‑1238, ‑3131, ‑3149, ‑1224‑3p, ‑3162‑3p, ‑532, ‑877, ‑4701‑5p, ‑5096, ‑4728‑3p, ‑1273d, ‑486‑3p and‑4763‑3p) was downregulated in the SGC‑7901/5‑Fu cell line compared with its parental cell line. The results indicate that miRNA expression correlates with MDR in gastric cancer and may serve as biomolecular targets for MDR elimination.
authors.affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Gu
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Li
authors.forename: Jianbin
authors.initials: J
authors.lastname: Xiang
authors.forename: Jianhai
authors.initials: J
authors.lastname: Jiang
authors.forename: Zongyou
authors.initials: Z
authors.lastname: Chen
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D018432:Drug Resistance, Multiple / Q000187:drug effects / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000235:genetics*; D005472:Fluorouracil / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D035683:MicroRNAs; D012333:RNA, Messenger; D005472:Fluorouracil