title: Production of enzymes by Paenibacillus chitinolyticus and Paenibacillus ehimensis to obtain chitooligosaccharides.
abstract: Obtaining oligosaccharides from chitosan has been the focus of several studies in the pharmaceutical, chemical, food, and medical areas, due to their functional properties. Here, we evaluated the production potential of biologically functional chitooligosaccharides using enzymes extracts produced by Paenibacillus chitinolyticus and Paenibacillus ehimensis. After 48 h of fermentation, these microorganisms were able to produce chitosanases, which generated oligomers with a degree of polymerization between dimers and hexamers. The maximum conversion of chitosan to oligomers was 99.2 %, achieved after 12 h incubation of chitosan with enzymes produced by P. ehimensis. The chitooligosaccharides generated were capable of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical, reaching a maximum scavenging rate of 61 and 39 % when produced with P. ehimensis and P. chitinolyticus enzymes, respectively. The use of these enzymes in the crude form could facilitate their use in industrial applications.
authors.affiliation: Departamento de Engenharia Química, Centro de Tecnologia, Universidade Federal do Rio Grande do Norte, CEP 59072-970, Natal, Brazil. nakar_rn@hotmail.com
authors.forename: Nathália Kelly
authors.initials: NK
authors.lastname: de Araújo
authors.forename: Cristiane Fernandes
authors.initials: CF
authors.lastname: de Assis
authors.forename: Everaldo Silvino
authors.initials: ES
authors.lastname: Dos Santos
authors.forename: Gorete Ribeiro
authors.initials: GR
authors.lastname: de Macedo
authors.forename: Louise Fonseca
authors.initials: LF
authors.lastname: de Farias
authors.forename: Humberto
authors.initials: H
authors.lastname: Arimatéia
authors.forename: Matheus
authors.initials: M
authors.lastname: de Freitas Fernandes Pedrosa
authors.forename: Maria Giovana Binder
authors.initials: MG
authors.lastname: Pagnoncelli
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000378:metabolism; D048271:Chitosan / Q000378:metabolism*; D004789:Enzyme Activation; D005285:Fermentation; D016166:Free Radical Scavengers / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000378:metabolism*; D056507:Paenibacillus / Q000201:enzymology*; D010851:Picrates / Q000378:metabolism; D058105:Polymerization
chemical_list: D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D010851:Picrates; D048271:Chitosan; C004931:1,1-diphenyl-2-picrylhydrazyl; D006026:Glycoside Hydrolases; C019868:chitosanase