title: Identification of novel tumour-associated antigens in canine mammary gland tumour.
abstract: Canine mammary gland tumour (MGT) is the most common neoplasm in female dogs and has similar biological characteristics to human MGT. Spontaneous canine MGT is a more attractive clinical model in oncological research than that of the murine experimental model. Tumour-associated antigens (TAAs), which are produced in tumour cells, are applied as tumour markers, tumour vaccine antigens and molecular targets of therapeutic drugs. In this study, we have primarily identified 13 different TAAs of canine MGT by serological immunoscreening of cDNA expression library. The results of serological mini-arrays of identified antigens showed that CCDC41 antigen specially reacted with 35% of sera from MGT-dogs and did not react with control sera. We also found that HSPH1 mRNA expression levels increased significantly in MGT tissues. These findings will contribute to the development of diagnostic technologies and translational target therapies for dogs.
  
  
  CONCLUSIONS
  HSPH1, which is strongly expressed in the tumour tissue, will be a possible vaccine antigen of canine MGT.
authors.affiliation: Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Furuya
authors.affiliation: Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Funasaki
authors.affiliation: Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tani
authors.affiliation: Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sasai
mesh_terms: D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics*; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D005260:Female; D015723:Gene Library; D007564:Japan; D015674:Mammary Neoplasms, Animal / Q000235:genetics*; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017421:Sequence Analysis / Q000662:veterinary
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D012333:RNA, Messenger
keywords: canine; immunoscreening; mammary gland tumour; serology; tumour-associated antigen