title: Optimization and validation of a GC-FID method for the determination of acetone-butanol-ethanol fermentation products.
abstract: An improved, simple gas chromatography-flame ionization detection (GC-FID) method was developed for measuring the products of acetone-butanol-ethanol (ABE) fermentation and the combined fermentation/separation processes. The analysis time per sample was reduced to less than 10 min compared to those of a conventional GC-FID (more than 20 min). The behavior of the compounds in temperature-programmed gas chromatographic runs was predicted using thermodynamic parameters derived from isothermal runs. The optimum temperature programming condition was achieved when the resolution for each peak met the analytical requirement and the analysis time was shortest. With the exception of acetic acid, the detection limits of the presented method for various products were below 10 mg/L. The repeatability and intermediate precision of the method were less than 10% (relative standard deviation). Validation and quantification results demonstrated that this method is a sensitive, reliable and fast alternative for conventional investigation of the adsorption-coupled ABE fermentation process.
authors.affiliation: 1State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Lin
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Fan
authors.forename: Qingshi
authors.initials: Q
authors.lastname: Wen
authors.forename: Renjie
authors.initials: R
authors.lastname: Li
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Jin
authors.forename: Jinglan
authors.initials: J
authors.lastname: Wu
authors.forename: Wenbin
authors.initials: W
authors.lastname: Qian
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xie
authors.forename: Jianxin
authors.initials: J
authors.lastname: Bai
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D000096:Acetone / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000440:Butanols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods*; D046969:Clostridium acetobutylicum / Q000378:metabolism; D000431:Ethanol / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D000440:Butanols; D000096:Acetone; D000431:Ethanol