title: Predictive value of prepartum serum metabolites for incidence of clinical and subclinical mastitis in grazing primiparous Holstein cows.
abstract: The aim of this study was to measure changes in biochemical markers in the peripartum period of primiparous Holstein cows diagnosed with subclinical and clinical mastitis. In this study, 37 dairy cows were monitored daily during milking until 60 days postpartum and were categorized according to the occurrence of clinical mastitis (group mastitis (GM), n = 9) or subclinical mastitis (group subclinical mastitis (GSUB), n = 10) or absence of symptoms (control group (CG), n = 18). Blood samples were collected weekly from -30 to 60 days from calving. Samples were grouped for prepartum (-30 to 0 days from calving), early postpartum (0 to 30 days from calving), and late postpartum (30 to 60 days from calving) periods. Prepartum serum non-esterified fatty acid (NEFA) concentration was higher in GM than in CG (P < 0.01). In addition, CG had higher prepartum serum glucose concentration than GM (P = 0.03). In the early postpartum period, aspartate aminotransferase (AST) activity was lower in CG than in GSUB (P < 0.05), and in the late postpartum period, AST activity was lower in CG than GSUB and GM (P = 0.01). Somatic cell count was higher during the early and late postpartum periods for GM and GSUB when compared to CG (P < 0.01). In this study, primiparous cows with low glucose and higher NEFA in the prepartum were more susceptible for mastitis in the early postpartum, probably due to low immune function associated to a more negative energy balance. In sum, increased prepartum serum NEFA concentration and decreased glucose in primiparous cows were associated with clinical mastitis incidence in the postpartum period.
authors.affiliation: Department of Veterinary Clinics, Veterinary College, Universidade Federal de Pelotas, Campus Universitário, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil, bethveterinaria@gmail.com.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Schwegler
authors.forename: Augusto
authors.initials: A
authors.lastname: Schneider
authors.forename: Paula
authors.initials: P
authors.lastname: Montagner
authors.forename: Diego Andres Velasco
authors.initials: DA
authors.lastname: Acosta
authors.forename: Luiz Francisco Machado
authors.initials: LF
authors.lastname: Pfeifer
authors.forename: Eduardo
authors.initials: E
authors.lastname: Schmitt
authors.forename: Viviane Rohrig
authors.initials: VR
authors.lastname: Rabassa
authors.forename: Francisco Augusto Burkert
authors.initials: FA
authors.lastname: Del Pino
authors.forename: Helenice
authors.initials: H
authors.lastname: de Lima Gonzalez
authors.forename: Cláudio Dias
authors.initials: CD
authors.lastname: Timm
authors.forename: Marcio Nunes
authors.initials: MN
authors.lastname: Corrêa
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood*; D058345:Asymptomatic Infections / Q000453:epidemiology; D015415:Biomarkers / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism*; D001938:Brazil / Q000453:epidemiology; D002417:Cattle / Q000378:metabolism*; D002452:Cell Count / Q000662:veterinary; D003124:Colorimetry / Q000662:veterinary; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D015994:Incidence; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology / Q000378:metabolism; D010298:Parity; D058725:Peripartum Period; D049590:Postpartum Period; D011247:Pregnancy; D014329:Tropical Climate
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D001219:Aspartate Aminotransferases