title: Genome-wide DNA methylation analysis using massively parallel sequencing technologies.
abstract: "Epigenetics" refers to a heritable change in transcriptional status without alteration in the primary nucleotide sequence. Epigenetics provides an extra layer of transcriptional control and plays a crucial role in normal development, as well as in pathological conditions. DNA methylation is one of the best known and well-studied epigenetic modifications. Genome-wide DNA methylation profiling has become recognized as a biologically and clinically important epigenomic assay. In this review, we discuss the strengths and weaknesses of the protocols for genome-wide DNA methylation profiling using massively parallel sequencing (MPS) techniques. We will also describe recently discovered DNA modifications, and the protocols to detect these modifications.
authors.affiliation: Center for Epigenomics, Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA. masako.suzuki@einstein.yu.edu
authors.forename: Masako
authors.initials: M
authors.lastname: Suzuki
authors.forename: John M
authors.initials: JM
authors.lastname: Greally
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans
chemical_list: D004247:DNA