title: A facile route to the synthesis of spherical poly(acrylic acid) brushes via RAFT polymerization for high-capacity protein immobilization.
abstract: Spherical poly(acrylic acid) brushes were prepared via a facile RAFT polymerization route from silica nanoparticles (SiO2@PAAs). A silane functionalized RAFT chain transfer agent was designed and synthesized by a one-step reaction, and then immobilized onto silica nanoparticles (SiNPs) through its R group to afford RAFT polymerization. Key structural parameters and contents of carboxyl groups of SiO2@PAAs were thoroughly characterized by transmission electron microscopy, dynamic light scattering, gel permeation chromatography, thermogravimetric analysis and conductometric titration. The SiO2@PAAs exhibit excellent dispersity, tunable brush thicknesses (14.6-68.8 nm) and abundant carboxyl groups (0.82-2.37 mmol/g). An ultra-high protein immobilization capacity (2600 μg streptavidin/mg SiO2@PAAs) was realized by virtue of its rich carboxyl groups and spherical brush structure, which opens up new possibilities for biomedical applications.
authors.affiliation: School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, PR China.
authors.forename: Zhenyuan
authors.initials: Z
authors.lastname: Qu
authors.forename: Fenglin
authors.initials: F
authors.lastname: Hu
authors.forename: Kaimin
authors.initials: K
authors.lastname: Chen
authors.forename: Zongqiang
authors.initials: Z
authors.lastname: Duan
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D055767:Immobilized Proteins; C006903:carbopol 940; D012822:Silicon Dioxide; D019809:Streptavidin