title: Glutamate carboxypeptidase II does not process amyloid-β peptide.
abstract: The accumulation of amyloid-β (Aβ) peptide is thought to be a major causative mechanism of Alzheimer's disease. Aβ accumulation could be caused by dysregulated processing of amyloid precursor protein, yielding excessive amounts of Aβ, and/or by inefficient proteolytic degradation of the peptide itself. Several proteases have been described as Aβ degradation enzymes, most notably metalloendopeptidases, aspartic endopeptidases, and some exopeptidases. Recently a report suggested that another metallopeptidase, glutamate carboxypeptidase II (GCPII), can also cleave Aβ. GCPII is a zinc exopeptidase that cleaves glutamate from N-acetyl-L-aspartyl-L-glutamate in the central nervous system and from pteroylpoly-γ-glutamate in the jejunum. GCPII has been proposed as a promising therapeutic target for disorders caused by glutamate neurotoxicity. However, an Aβ-degrading activity of GCPII would compromise potential pharmaceutical use of GCPII inhibitors, because the enzyme inhibition might lead to increased Aβ levels and consequently to Alzheimer's disease. Therefore, we analyzed the reported Aβ-degrading activity of GCPII using highly purified recombinant enzyme and synthetic Aβ. We did not detect any Aβ degradation activity of GCPII or its homologue even under prolonged incubation at a high enzyme to substrate ratio. These results are in good agreement with the current detailed structural understanding of the substrate specificity and enzyme-ligand interactions of GCPII.
authors.affiliation: Gilead Sciences, Prague, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Sedlák
authors.forename: Pavel
authors.initials: P
authors.lastname: Šácha
authors.forename: Miroslava
authors.initials: M
authors.lastname: Blechová
authors.forename: Anna
authors.initials: A
authors.lastname: Březinová
authors.forename: Martin
authors.initials: M
authors.lastname: Šafařík
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Šebestík
authors.forename: Jan
authors.initials: J
authors.lastname: Konvalinka
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis / Q000187:drug effects; D020134:Catalytic Domain; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000378:metabolism; D043425:Glutamate Carboxypeptidase II / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry; D015394:Molecular Structure; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D059748:Proteolysis; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D014316:Tritium
chemical_list: C402107:2-(phosphonomethyl)pentanedioic acid; D016229:Amyloid beta-Peptides; D000954:Antigens, Surface; D004151:Dipeptides; D009943:Organophosphorus Compounds; D010446:Peptide Fragments; D011994:Recombinant Proteins; C075222:amyloid beta-protein (1-42); D014316:Tritium; C027172:isospaglumic acid; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II; D015260:Neprilysin
keywords: Alzheimer's disease; PSMA; depsipeptide; disaggregation; exopeptidase; substrate specificity