title: Antioxidant effect of salidroside and its protective effect against furan-induced hepatocyte damage in mice.
abstract: Furan, a widely used industrial compound, has been found in many heat-treated foods, especially baby food. The presence of furan in food raises concerns about public health. In this study, we evaluated the protective effect of salidroside (SR) against furan-induced hepatocyte damage in mice livers. The in vitro antioxidant effects of SR were also evaluated. The results suggested that furan significantly increased hepatocyte damage, as proven by the increased activities of aminotransferase (AST), alanine aminotransferase (ALT) and levels of direct bilirubin (DBIL). Furan also caused oxidative stress, as evidenced by increased reactive oxygen species (ROS) production as well as malondialdehyde (MDA) levels and glutathione S-transferase (GST) activity in mice livers. Pretreatment with SR markedly attenuated the activities of AST, ALT, GST and the levels of DBIL, ROS, and MDA in a dose-dependent manner. The protective effects of SR against furan-induced hepatocyte damage were due to its excellent ability to scavenge free radicals such as 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radicals, ˙OH, 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl radicals and ˙O(2)(-). Thus, SR exerted excellent antioxidant effects, and it may be a novel therapeutic and preventive agent for oxidative stress-related diseases.
authors.affiliation: College of Quartermaster Technology, Jilin University, Changchun, China, 130062. yuanyuan1024@gmail.com
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Si-Jia
authors.initials: SJ
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Zhang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001663:Bilirubin / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000633:toxicity*; D005960:Glucosides / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000097:blood; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000097:blood; D013858:Thiobarbiturates / Q000032:analysis; D000637:Transaminases / Q000097:blood
chemical_list: D000975:Antioxidants; D005663:Furans; D005960:Glucosides; D010636:Phenols; D017382:Reactive Oxygen Species; D013858:Thiobarbiturates; D008315:Malondialdehyde; D005982:Glutathione Transferase; D000637:Transaminases; D000410:Alanine Transaminase; C029684:thiobarbituric acid; C009172:rhodioloside; D001663:Bilirubin; C039281:furan