title: N-methylimidazolium modified magnetic particles as adsorbents for solid phase extraction of genomic deoxyribonucleic acid from genetically modified soybeans.
abstract: N-Methylimidazolium modified magnetic particles (MIm-MPs) were prepared and applied in the solid phase extraction of genomic deoxyribonucleic acid (DNA) from genetically modified soybeans. The adsorption of MIm-MPs for DNA mainly resulted from the strong electrostatic interaction between the positively charged MPs and the negatively charged DNA. The elution of DNA from MPs-DNA conjugates using phosphate buffer resulted from the stronger electrostatic interaction of phosphate ions with MPs than DNA. In the extraction procedure, no harmful reagents (e.g. phenol, chloroform and isopropanol, etc.) used, high yield (10.4 μg DNA per 30 mg sample) and high quality (A260/A280=1.82) of DNA can be realized. The as-prepared DNA was used as template for duplex-polymerase chain reaction (PCR) and the PCR products were analyzed by a sieving capillary electrophoresis method. Quick and high quality extraction of DNA template, and fast and high resolution detection of duplex PCR products can be realized using the developed method. No toxic reagents are used throughout the method.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Manchen
authors.initials: M
authors.lastname: Deng
authors.forename: Cheng
authors.initials: C
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Jia
mesh_terms: D000327:Adsorption; D001483:Base Sequence; D018744:DNA, Plant / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D018745:Genome, Plant / Q000235:genetics*; D007093:Imidazoles / Q000737:chemistry*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D052616:Solid Phase Extraction / Q000379:methods*; D013025:Glycine max / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D007093:Imidazoles; D052203:Ferrosoferric Oxide