title: Display of Neospora caninum surface protein related sequence 2 on Rous sarcoma virus-derived gag protein virus-like particles.
abstract: Virus-like particles (VLPs) displaying antigen have been increasingly recognized as a potential vaccine in the livestock industry. In this study, Neospora caninum surface protein related sequence (NcSRS)2 was displayed on the surface of Rous sarcoma virus group-antigen protein (RSV-gag) VLPs. Two types of Bombyx mori nucleopolyhedrovirus (BmNPV) bacmids, encoding RSV-gag and NcSRS2 genes, were co-injected into silkworm larvae to produce VLPs-NcSRS2. At 7 days post-injection, VLPs-NcSRS2 were collected from hemolymph and purified. The antigenicity of the purified protein was confirmed by enzyme-linked immunosorbent assay (ELISA) using neosporosis-positive bovine serum. ELISA revealed that ~0.16μg rNcSRS2 was displayed per 1μg VLPs-NcSRS2. To develop an antibody specific for VLPs-NcSRS2, purified VLPs-NcSRS2 were used to immunize mice in a three-dose regimen without adjuvant and the production of antibodies was confirmed in serum samples. By using a silkworm expression system, we demonstrated the display, expression and immunization of neosporosis-targeting membrane proteins, which are vaccine candidates for neosporosis.
authors.affiliation: Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
authors.forename: Vipin Kumar
authors.initials: VK
authors.lastname: Deo
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Takahiro
authors.initials: T
authors.lastname: Otsuki
authors.forename: Jinhua
authors.initials: J
authors.lastname: Dong
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kato
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics* / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics* / Q000378:metabolism; D012831:Bombyx / Q000235:genetics; D002417:Cattle; D003048:Coccidiosis / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D015683:Gene Products, gag / Q000235:genetics* / Q000378:metabolism; D018688:Neospora / Q000235:genetics / Q000378:metabolism / Q000821:virology; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D052736:Rous sarcoma virus / Q000235:genetics / Q000378:metabolism; D058425:Vaccines, Virus-Like Particle / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015683:Gene Products, gag; D015800:Protozoan Proteins; C115389:SRS2 protein, Neospora caninum; D058425:Vaccines, Virus-Like Particle