title: Heparin-binding epidermal growth factor-like growth factor protects rat intestine after portal triad clamping.
abstract: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a potent mitogen and chemotactic factor. HB-EGF attenuates intestinal ischemia/reperfusion injury caused by superior mesenteric artery occlusion. We examined whether HB-EGF offers protection against intestinal congestion/reperfusion (C/R) injury, which is caused by portal triad clamping. Male Sprague-Dawley rats were randomly divided into three equally sized groups: I, sham-operated; II, portal triad clamping (Pringle maneuver); III, II + intraluminal administration of HB-EGF. Compared with sham-operated rats, all rats in group II exhibited significant increases in intestinal histologic injury, pro-inflammatory cytokine expression, myeloperoxidase activity, malonaldehyde levels, and apoptosis indices. Intraluminal administration of HB-EGF in group III significantly reduced these indicators when compared with group II. Clamping of the portal triad followed by reperfusion causes intestinal C/R injury and intraluminal administration of HB-EGF reduces the severity of intestinal C/R injury in rats.
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Republic of China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Liao
authors.forename: Liyan
authors.initials: L
authors.lastname: Chen
authors.forename: Wenguang
authors.initials: W
authors.lastname: Fu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D003250:Constriction; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology; D008297:Male; D011168:Portal System / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
chemical_list: C587883:HBEGF protein, human; C587885:Hbegf protein, rat; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins