title: Genetic structure, polymorphism identification of LGP2 gene and their relationship with the resistance/susceptibility to GCRV in grass carp, Ctenopharyngodon idella.
abstract: Laboratory of genetics and physiology 2 (LGP2) is an actual detector and regulator during RNA viral infection in innate immunity. In this study, 5'-flanking region and all introns of LGP2 in grass carp (Ctenopharyngodon idella) were excavated. The genomic CiLGP2 (C. idella LGP2) was 8062bp in length, with a 364bp 5'-flanking region, twelve exons and eleven introns. Besides, the promoter activity of the upstream region before initiator codon was identified. By sequencing, six single nucleotide polymorphisms (SNPs) and one 20-bp insertion/deletion polymorphism were detected in CiLGP2. With a challenge experiment, the genotype and allele distributions of these seven polymorphisms were examined. Analytic result revealed only the -1392 C/G, 494 A/T and 4403 C/T loci were significantly associated with the resistance/susceptibility to grass carp reovirus (GCRV) (P<0.05). To further identify these correlations, another independent challenge test was performed. The analytic result based on the cumulative mortality demonstrated that the stock in -1392 GG genotype was more susceptible to GCRV than that in CC genotype, while the stocks in 494 TT genotype and 4403 TT genotype were more resistant to GCRV than that in AA and CC genotype stocks, respectively (P<0.05). Those significant SNPs might be potential gene markers for the future molecular selection of C. idella strains that are resistant to GCRV.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China.
authors.forename: Quanyuan
authors.initials: Q
authors.lastname: Wan
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Su
authors.forename: Chunrong
authors.initials: C
authors.lastname: Yang
authors.forename: Limin
authors.initials: L
authors.lastname: Peng
authors.forename: Lijun
authors.initials: L
authors.lastname: Chen
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D005393:Fish Diseases / Q000235:genetics* / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D007438:Introns; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012087:Reoviridae / Q000472:pathogenicity*; D012088:Reoviridae Infections / Q000235:genetics / Q000662:veterinary
chemical_list: D029941:Fish Proteins; D020365:RNA Helicases