title: Transgene delivery via intracellular electroporetic nanoinjection.
abstract: Development of an effective cytoplasmic delivery technique has remained an elusive goal for decades despite the success of pronuclear microinjection. Cytoplasmic injections are faster and easier than pronuclear injection and do not require the pronuclei to be visible; yet previous attempts to develop cytoplasmic injection have met with limited success. In this work we report a cytoplasmic delivery method termed intracellular electroporetic nanoinjection (IEN). IEN is unique in that it manipulates transgenes using electrical forces. The microelectromechanical system (MEMS) uses electrostatic charge to physically pick up transgenes and place them in the cytoplasm. The transgenes are then propelled through the cytoplasm and electroporated into the pronuclei using electrical pulses. Standard electroporation of whole embryos has not resulted in transgenic animals, but the MEMS device allows localized electroporation to occur within the cytoplasm for transgene delivery from the cytoplasm to the pronucleus. In this report we describe the principles which allow localized electroporation of the pronuclei including: the location of mouse pronuclei between 21 and 28 h post-hCG treatment, modeling data predicting the voltages needed for localized electroporation of pronuclei, and data on electric-field-driven movement of transgenes. We further report results of an IEN versus microinjection comparative study in which IEN produced transgenic pups with viability, transgene integration, and expression rates statistically comparable to microinjection. The ability to perform injections without visualizing or puncturing the pronuclei will widely benefit transgenic research, and will be particularly advantageous for the production of transgenic animals with embryos exhibiting reduced pronuclear visibility.
authors.affiliation: Microbiology and Molecular Biology Department, 775 WIDB, Brigham Young University, Provo, UT, 84602, USA.
authors.forename: Aubrey M
authors.initials: AM
authors.lastname: Wilson
authors.forename: Quentin T
authors.initials: QT
authors.lastname: Aten
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Toone
authors.forename: Justin L
authors.initials: JL
authors.lastname: Black
authors.forename: Brian D
authors.initials: BD
authors.lastname: Jensen
authors.forename: Susan
authors.initials: S
authors.lastname: Tamowski
authors.forename: Larry L
authors.initials: LL
authors.lastname: Howell
authors.forename: Sandra H
authors.initials: SH
authors.lastname: Burnett
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D004622:Embryo, Mammalian; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D008845:Microinjections; D018613:Microscopy, Confocal; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D019076:Transgenes / Q000235:genetics*
chemical_list: D017931:DNA Primers; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins