title: Assessment of base-excision repair activity in fish cell lines: toward a new biomarker of exposure to environmental contaminants?
abstract: Amongst DNA-repair processes, base-excision repair (BER) is the major mechanism for removal of DNA-base lesions caused by environmental genotoxicants. BER has been proven to exist in fish but has not been investigated in fish cell-lines, although these constitute increasingly important tools in eco-toxicological assessment. The present study aims at highlighting BER capacity of RTL-W1 and RTG-W1, two trout cell lines used in eco-genotoxicity studies. This is realized by following the kinetics of strand-break repair after a short exposure to model genotoxicants-leading predominantly to BER-specific lesions-by means of the standard alkaline and Fpg-modified comet assays. Results show that both cell lines efficiently repair single-strand breaks and base-alkylation damages within 4h and 24h, respectively. Then, the study shows that after minor modifications of the protocol, the cell extract-based BERc assay can be used to evaluate the base-incision capacity of the cell lines and its variation after exposure of the cells to a model inhibitor of BER (3-aminobenzamide) and to environmental contaminants such as cadmium and tributyltin. This work provides a basis for the further development of DNA-repair activity in fish cell-lines as a new biomarker of genotoxicity.
authors.affiliation: Université de Lyon, Villeurbanne, France. aude.kienzler@yahoo.fr
authors.forename: Aude
authors.initials: A
authors.lastname: Kienzler
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bony
authors.forename: Xavier
authors.initials: X
authors.lastname: Tronchère
authors.forename: Alain
authors.initials: A
authors.lastname: Devaux
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D020552:Comet Assay / Q000379:methods; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects*; D054750:Ecotoxicology / Q000379:methods; D004785:Environmental Pollutants / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000633:toxicity; D007700:Kinetics; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009152:Mutagenicity Tests; D017686:Oncorhynchus mykiss; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D008741:Methyl Methanesulfonate; D006861:Hydrogen Peroxide