title: Characteristics of polyomavirus BK (BKPyV) infection in primary human urothelial cells.
abstract: High-level polyomavirus BK (BKPyV) replication in urothelial cells is a hallmark of polyomavirus-associated hemorrhagic cystitis (PyVHC), a painful condition affecting bone marrow transplant recipients. In kidney transplant recipients, replication in tubular epithelial cells is associated with overt disease whereas high-level urothelial replication is clinically silent. We characterized BKPyV replication in primary human urothelial cells (HUCs) and compared it to replication in renal tubular epithelial cells (RPTECs). HUCs were easily infected, as shown by expression of T-antigens, VP1-3, and agnoprotein, and intranuclear virion production. Compared to RPTECs, progeny release was delayed by ≥24h and reduced. BKPyV-infected HUCs rounded up like "decoy cells" and detached without necrosis as shown by delayed cytokeratin-18 release, real-time viability monitoring and imaging. The data show that BKV infection of HUCs and RPTECs is significantly different and support the notion that PyVHC pathogenesis is not solely due to BKPyV replication, but likely requires urotoxic and immunological cofactors.
authors.affiliation: Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway.
authors.forename: Ruomei
authors.initials: R
authors.lastname: Li
authors.forename: Biswa Nath
authors.initials: BN
authors.lastname: Sharma
authors.forename: Stig
authors.initials: S
authors.lastname: Linder
authors.forename: Tore Jarl
authors.initials: TJ
authors.lastname: Gutteberg
authors.forename: Hans H
authors.initials: HH
authors.lastname: Hirsch
authors.forename: Christine Hanssen
authors.initials: CH
authors.lastname: Rinaldo
mesh_terms: D001739:BK Virus / Q000502:physiology*; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000648:ultrastructure / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D019459:Urothelium / Q000166:cytology* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication / Q000502:physiology*
chemical_list: D014764:Viral Proteins