title: HPTLC method for simultaneous analysis of stevioside and rebaudioside-A in Stevia rebaudiana.
abstract: An HPTLC method was developed and validated for simultaneous determination of stevioside (STE) and rebaudioside-A (REB-A) in Stevia rebaudiana leaves. The HPTLC separation was performed on precoated silica gel 60F254 HPTLC plates with the mobile phase ethyl acetate-ethanol-acetone-water (15 + 3 + 6 + 6, v/v/v/v). The densitometric quantification of steviol glycosides was carried out at Amax 580 nm in the reflection-absorption mode after spraying with anisaldehyde-sulfuric acid reagent. Rf values were 0.34 and 0.28 for STE and REB-A, respectively. The content of STE and REB-A in leaf extract was found to be 6.94 and 6.35%, respectively. The method was validated in terms of precision, accuracy, specificity, and robustness. The method can be useful for routine analysis of the sweeteners.
authors.affiliation: Sinhgad College of Pharmacy, Department of Pharmaceutical Chemistry, Vadgaon, Pune- 411041, India. smitaksl@rediffmail.com
authors.forename: Smita V
authors.initials: SV
authors.lastname: Londhe
authors.forename: Sadhana M
authors.initials: SM
authors.lastname: Nanaware
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D045786:Diterpenes, Kaurane / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D036625:Stevia / Q000737:chemistry*
chemical_list: D045786:Diterpenes, Kaurane; D005960:Glucosides; C012043:stevioside; C025474:rebaudioside A