title: Antiresorptive activity of bacillus-fermented antler extracts: inhibition of osteoclast differentiation.
abstract: Antlers have been traditionally used for thousands of years as a natural product with medicinal and pharmaceutical properties. In developing healthy foods, Bacillus-mediated fermentation is widely used to enhance the biological activity of nutrients in foods. Recently, fermentation was shown to enhance the osteogenic activity of antlers. This study aimed to elucidate the antiresorptive activity of Bacillus-fermented antler and its mode of action. We found that Bacillus-fermented antler extract strongly inhibited osteoclast differentiation by downregulating the expression and activity of nuclear factor of activated T cells, cytoplasmic 1 (NFATc1). This extract also inhibited the activation of phospholipase C γ 2 (PLC γ 2), a signaling molecule that could regulate NFATc1 transcriptional activity. This suggested that Bacillus-fermented antler extract could inhibit PLC γ 2-NFATc1 signaling required for bone resorption and cell fusion. Consequently, Bacillus-fermented antler extract might benefit osteoclast-related disorders, including osteoporosis; furthermore, it may improve gastrointestinal activity.
authors.affiliation: Laboratory of Translational Therapeutics, Pharmacology Research Center, Bio-Organic Science Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors.forename: Sik-Won
authors.initials: SW
authors.lastname: Choi
authors.forename: Seong-Hee
authors.initials: SH
authors.lastname: Moon
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Son
authors.forename: Ri
authors.initials: R
authors.lastname: Yu
authors.forename: Young Su
authors.initials: YS
authors.lastname: Kim
authors.forename: So I
authors.initials: SI
authors.lastname: Kim
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Chae
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Park
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim