title: Oxidative DNA damage corresponds to the long term survival of human cells treated with silver nanoparticles.
abstract: We examined the relation between DNA damage and the clonogenic potential of 3 human cell lines, HepG2, HT29 and A549, treated with bare 20 nm or 200 nm silver nanoparticles (AgNPs). The endpoints examined were the DNA breakage estimated by the comet assay, the oxidative base damage recognized by formamido-pyrimidine glycosylase (FPG) and estimated with the FPG+comet assay, and the frequencies of histone γH2AX foci and micronuclei. Each cell line studied had a different pattern of DNA breakage and base damage versus the NPs concentration and time of treatment. The overall pattern of DNA breakage and base damage induction corresponded to the intracellular generation of reactive oxygen species. There was no increase in the frequencies of histone γH2AX foci and micronuclei as compared to those in the untreated cells. The reported experiments suggest that only the oxidative DNA damage corresponds to the loss of the clonogenic ability of cells treated with AgNPs.
authors.affiliation: Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Warszawa, Poland. m.kruszewski@ichtj.waw.pl
authors.forename: Marcin
authors.initials: M
authors.lastname: Kruszewski
authors.forename: Iwona
authors.initials: I
authors.lastname: Grądzka
authors.forename: Teresa
authors.initials: T
authors.lastname: Bartłomiejczyk
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Chwastowska
authors.forename: Sylwester
authors.initials: S
authors.lastname: Sommer
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grzelak
authors.forename: Mariusz
authors.initials: M
authors.lastname: Zuberek
authors.forename: Anna
authors.initials: A
authors.lastname: Lankoff
authors.forename: Maria
authors.initials: M
authors.lastname: Dusinska
authors.forename: Maria
authors.initials: M
authors.lastname: Wojewódzka
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D020552:Comet Assay; D003470:Culture Media; D004249:DNA Damage; D023381:Endpoint Determination; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D019073:HT29 Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D009153:Mutagens; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry / Q000633:toxicity*; D014965:X-Rays
chemical_list: D003470:Culture Media; D006657:Histones; D009153:Mutagens; D017382:Reactive Oxygen Species; D012834:Silver