title: Oceanobacillus polygoni sp. nov., a facultatively alkaliphile isolated from indigo fermentation fluid.
abstract: A facultatively alkaliphilic, lactic-acid-producing and halophilic strain, designated SA9(T), was isolated from a fermented Polygonum indigo (Polygonum tinctorium Lour.) liquor sample prepared in a laboratory. The 16S rRNA gene sequence phylogeny suggested that strain SA9(T) was a member of the genus Oceanobacillus with the closest relative being Oceanobacillus profundus KCCM 42318(T) (99.3% 16S rRNA gene sequence similarity). Cells of strain SA9(T) stained Gram-positive and were facultative anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 5 and 48 °C (optimum, 35 °C) and at pH 7-12 (optimum, pH 9). The isoprenoid quinone detected was menaquinone-7 (MK-7) and the DNA G+C content was 40.6 ± 0.9 mol%. The whole-cell fatty acid profile mainly consisted of iso-C(15:0), anteiso-C(15:0), C(16:0) and anteiso-C(17:0). DNA-DNA hybridization with Oceanobacillus profundus DSM 18246(T) revealed a DNA-DNA relatedness value of 23 ± 2%. On the basis of the differences in phenotypic and chemotaxonomic characteristics, and the results of phylogenetic analyses based on 16S rRNA gene sequences and DNA-DNA relatedness data from recognized species of the genus Oceanobacillus, strain SA9(T) merits classification as a representative of a novel species of the genus Oceanobacillus, for which the name Oceanobacillus polygoni sp. nov. is proposed. The type strain is SA9(T) ( =JCM 17252(T) =NCIMB 14684(T)). An emended description of the genus Oceanobacillus is also provided.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors.forename: Kikue
authors.initials: K
authors.lastname: Hirota
authors.affiliation: Laboratory of Environmental Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Hanaoka
authors.affiliation: Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nodasaka
authors.affiliation: Laboratory of Environmental Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Yumoto
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D007203:Indigo Carmine / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D028502:Polygonum / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7; D007203:Indigo Carmine