title: ANP and BNP responses to dehydration in the one-humped camel and effects of blocking the renin-angiotensin system.
abstract: The objectives of this study were to investigate and compare the responses of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) in the circulation of hydrated, dehydrated, and dehydrated losartan - treated camels; and to document the cardiac storage form of B-type natriuretic peptide in the camel heart. Eighteen male camels were used in the study: control or hydrated camels (n = 6), dehydrated camels (n = 6) and dehydrated losartan-treated camels (n = 6) which were dehydrated and received the angiotensin II (Ang II) AT-1 receptor blocker, losartan, at a dose of 5 mg/kg body weight intravenously for 20 days. Control animals were supplied with feed and water ad-libitum while both dehydrated and dehydrated-losartan treated groups were supplied with feed ad-libitum but no water for 20 days. Compared with time-matched controls, dehydrated camels exhibited a significant decrease in plasma levels of both ANP and BNP. Losartan-treated camels also exhibited a significant decline in ANP and BNP levels across 20 days of dehydration but the changes were not different from those seen with dehydration alone. Size exclusion high performance liquid chromatography of extracts of camel heart indicated that proB-type natriuretic peptide is the storage form of the peptide. We conclude first, that dehydration in the camel induces vigorous decrements in circulating levels of ANP and BNP; second, blockade of the renin-angiotensin system has little or no modulatory effect on the ANP and BNP responses to dehydration; third, proB-type natriuretic peptide is the storage form of this hormone in the heart of the one-humped camel.
authors.affiliation: Department of Pharmacology, Faculty of Medicine and Health Sciences, UAE University, Al Ain, United Arab Emirates.
authors.forename: Abdu
authors.initials: A
authors.lastname: Adem
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Al Haj
authors.forename: Sheela
authors.initials: S
authors.lastname: Benedict
authors.forename: Javed
authors.initials: J
authors.lastname: Yasin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Nagelkerke
authors.forename: Fred
authors.initials: F
authors.lastname: Nyberg
authors.forename: Tim G
authors.initials: TG
authors.lastname: Yandle
authors.forename: Chris M
authors.initials: CM
authors.lastname: Frampton
authors.forename: Lynley K
authors.initials: LK
authors.lastname: Lewis
authors.forename: M Gary
authors.initials: MG
authors.lastname: Nicholls
authors.forename: Elsadig
authors.initials: E
authors.lastname: Kazzam
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism*; D002162:Camelus / Q000502:physiology*; D003681:Dehydration / Q000188:drug therapy / Q000378:metabolism*; D019808:Losartan / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000378:metabolism; D020097:Natriuretic Peptide, Brain / Q000097:blood / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000187:drug effects / Q000502:physiology*
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor; D019808:Losartan