title: Extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Meyer) using commercial enzymes and high hydrostatic pressure.
abstract: A combination of high hydrostatic pressure (HHP) and enzymatic hydrolysis (HHP-EH) was applied for the extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Myer). The highest yield of ginsenosides was obtained by using a mixture of three enzymes (Celluclast + Termamyl + Viscozyme) along with HHP (100 MPa, at 50 °C for 12 h) in comparison to control samples (no enzymes, atmosphere pressure, P < 0.05). Total ginsenosides increased by 184% while Rg1 + Rb1 increased by 273%. Application of these conditions significantly increased total ginsenosides by 49% and Rg1 + Rb1 by 103% compared to HHP treatment alone (P < 0.05). The effect of HHP on increased yield of ginsenosides is likely due in part, to acceleration of enzyme activity. Thus HHP-EH significantly improves the extraction of ginsenosides from fresh ginseng roots.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Science, 3-142G Katz Group Centre for Pharmacy & Health Research, University of Alberta, Edmonton, AB, Canada. hsunwoo@ualberta.ca
authors.forename: Hoon H
authors.initials: HH
authors.lastname: Sunwoo
authors.forename: Chong-Tai
authors.initials: CT
authors.lastname: Kim
authors.forename: Do-Yeon
authors.initials: DY
authors.lastname: Kim
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Maeng
authors.forename: Chang-Won
authors.initials: CW
authors.lastname: Cho
authors.forename: Soo-Jeong
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D001709:Biotechnology / Q000379:methods; D004798:Enzymes / Q000378:metabolism*; D036145:Ginsenosides / Q000302:isolation & purification*; D006874:Hydrostatic Pressure; D005894:Panax / Q000737:chemistry*
chemical_list: D004798:Enzymes; D036145:Ginsenosides