title: Enhanced sialylation and in vivo efficacy of recombinant human α-galactosidase through in vitro glycosylation.
abstract: Human α-galactosidase A (GLA) has been used in enzyme replacement therapy for patients with Fabry disease. We expressed recombinant GLA from Chinese hamster ovary cells with very high productivity. When compared to an approved GLA (agalsidase beta), its size and charge were found to be smaller and more neutral. These differences resulted from the lack of terminal sialic acids playing essential roles in the serum half-life and proper tissue targeting. Because a simple sialylation reaction was not enough to increase the sialic acid content, a combined reaction using galactosyltransferase, sialyltransferase, and their sugar substrates at the same time was developed and optimized to reduce the incubation time. The product generated by this reaction had nearly the same size, isoelectric points, and sialic acid content as agalsidase beta. Furthermore, it had better in vivo efficacy to degrade the accumulated globotriaosylceramide in target organs of Fabry mice compared to an unmodified version.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Sohn
authors.forename: Jung Mi
authors.initials: JM
authors.lastname: Lee
authors.forename: Heung-Rok
authors.initials: HR
authors.lastname: Park
authors.forename: Sung-Chul
authors.initials: SC
authors.lastname: Jung
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000519:alpha-Galactosidase; D019158:N-Acetylneuraminic Acid