title: Isolation and characterization of novel, highly proliferative human CD34/CD73-double-positive testis-derived stem cells for cell therapy.
abstract: Human adult stem cells are a readily available multipotent cell source that can be used in regenerative medicine. Despite many advantages, including low tumorigenicity, their rapid senescence and limited plasticity have curtailed their use in cell-based therapies. In this study, we isolated CD34/CD73-double-positive (CD34(+)/CD73(+)) testicular stromal cells (HTSCs) and found that the expression of CD34 was closely related to the cells' stemness and proliferation. The CD34(+)/CD73(+) cells grew in vitro for an extended period of time, yielding a multitude of cells (5.6×10(16) cells) without forming tumors in vivo. They also differentiated into all three germ layer lineages both in vitro and in vivo, produced cartilage more efficiently compared to bone marrow stem cells and, importantly, restored erectile function in a cavernous nerve crush injury rat model. Thus, these HTSCs may represent a promising new autologous cell source for clinical use.
authors.affiliation: Fertility Center, CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea.
authors.forename: Won Yun
authors.initials: WY
authors.lastname: Choi
authors.forename: Hwang Gyun
authors.initials: HG
authors.lastname: Jeon
authors.forename: Young
authors.initials: Y
authors.lastname: Chung
authors.forename: Jung Jin
authors.initials: JJ
authors.lastname: Lim
authors.forename: Dong Hyuk
authors.initials: DH
authors.lastname: Shin
authors.forename: Jung Mo
authors.initials: JM
authors.lastname: Kim
authors.forename: Byeong Seong
authors.initials: BS
authors.lastname: Ki
authors.forename: Seung-Hun
authors.initials: SH
authors.lastname: Song
authors.forename: Seong-Jun
authors.initials: SJ
authors.lastname: Choi
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Shim
authors.forename: Jisook
authors.initials: J
authors.lastname: Moon
authors.forename: Sung Jun
authors.initials: SJ
authors.lastname: Jung
authors.forename: Hyun Mi
authors.initials: HM
authors.lastname: Kang
authors.forename: Seah
authors.initials: S
authors.lastname: Park
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Jung Jae
authors.initials: JJ
authors.lastname: Ko
authors.forename: Kwang Yul
authors.initials: KY
authors.lastname: Cha
authors.forename: Tae Ki
authors.initials: TK
authors.lastname: Yoon
authors.forename: Haekwon
authors.initials: H
authors.lastname: Kim
authors.forename: Dong Ryul
authors.initials: DR
authors.lastname: Lee
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000328:Adult; D053687:Adult Stem Cells / Q000502:physiology* / Q000637:transplantation; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D053713:Azoospermia / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D002478:Cells, Cultured; D007172:Erectile Dysfunction / Q000628:therapy; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D008297:Male; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008875:Middle Aged; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013724:Teratoma / Q000473:pathology; D013737:Testis / Q000473:pathology; D059467:Transcriptome; D016896:Treatment Outcome
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D058851:GPI-Linked Proteins; D007328:Insulin; D015720:5'-Nucleotidase; C548241:NT5E protein, human