title: The specificity of the interaction between αB-crystallin and desmin filaments and its impact on filament aggregation and cell viability.
abstract: CRYAB (αB-crystallin) is expressed in many tissues and yet the R120G mutation in CRYAB causes tissue-specific pathologies, namely cardiomyopathy and cataract. Here, we present evidence to demonstrate that there is a specific functional interaction of CRYAB with desmin intermediate filaments that predisposes myocytes to disease caused by the R120G mutation. We use a variety of biochemical and biophysical techniques to show that plant, animal and ascidian small heat-shock proteins (sHSPs) can interact with intermediate filaments. Nevertheless, the mutation R120G in CRYAB does specifically change that interaction when compared with equivalent substitutions in HSP27 (R140G) and into the Caenorhabditis elegans HSP16.2 (R95G). By transient transfection, we show that R120G CRYAB specifically promotes intermediate filament aggregation in MCF7 cells. The transient transfection of R120G CRYAB alone has no significant effect upon cell viability, although bundling of the endogenous intermediate filament network occurs and the mitochondria are concentrated into the perinuclear region. The combination of R120G CRYAB co-transfected with wild-type desmin, however, causes a significant reduction in cell viability. Therefore, we suggest that while there is an innate ability of sHSPs to interact with and to bind to intermediate filaments, it is the specific combination of desmin and CRYAB that compromises cell viability and this is potentially the key to the muscle pathology caused by the R120G CRYAB.
authors.affiliation: School of Biological and Biomedical Sciences, The University of Durham, South Road, Durham DH1 3LE, UK.
authors.forename: Jayne L
authors.initials: JL
authors.lastname: Elliott
authors.forename: Ming
authors.initials: M
authors.lastname: Der Perng
authors.forename: Alan R
authors.initials: AR
authors.lastname: Prescott
authors.forename: Karin A
authors.initials: KA
authors.lastname: Jansen
authors.forename: Gijsje H
authors.initials: GH
authors.lastname: Koenderink
authors.forename: Roy A
authors.initials: RA
authors.lastname: Quinlan
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D006224:Cricetinae; D003893:Desmin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D050888:Heat-Shock Proteins, Small; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009154:Mutation; D011485:Protein Binding; D011487:Protein Conformation; D013696:Temperature; D014746:Vimentin / Q000235:genetics / Q000378:metabolism; D038203:alpha-Crystallin B Chain / Q000235:genetics / Q000378:metabolism*
chemical_list: D003893:Desmin; D005904:Glial Fibrillary Acidic Protein; D050888:Heat-Shock Proteins, Small; D014746:Vimentin; D038203:alpha-Crystallin B Chain