title: Genetic characterization of a border disease virus isolate originating from Slovakia.
abstract: In this study, a major part of genome of the pestivirus isolate 297 from Slovakia, comprising the 7195 nt-long 5΄-UTR-NS3 region was sequenced and analyzed. Conserved cleavage sites between individual viral proteins of this region were determined and the number of amino acids of respective proteins was estimated as follows: 168 for Npro, 100 for C, 227 for Erns, 195 for E1, 373 for E2, 70 for p7, 453 for NS2, and 683 for NS3. Based on sequence and phylogenetic analysis of 5΄-UTR, Npro, and E2 the isolate 297 was characterized as a border disease virus of genotype 3. It was found to be distinct from other BDV-3 strains analyzed so far, consequently forming a distinct branch within the phylogenetic clade. All these data expand a relatively limited knowledge of genetic properties of individual BDV genotypes and strains circulating in the Central Europe.
authors.affiliation: Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic.
authors.forename: V
authors.initials: V
authors.lastname: Lešková
authors.forename: A
authors.initials: A
authors.lastname: Jacková
authors.forename: M
authors.initials: M
authors.lastname: Vlasáková
authors.forename: S
authors.initials: S
authors.lastname: Vilček
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001882:Border Disease / Q000821:virology*; D018083:Border disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012756:Sheep; D018154:Slovakia; D013045:Species Specificity; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D020121:5' Untranslated Regions; D018076:DNA, Complementary; D012367:RNA, Viral; D014764:Viral Proteins