title: M-Current Recording from Acute DRG Slices.
abstract: Electrophysiological recordings from an acutely sliced preparation provide information on ionic currents and excitability of native neurons under near physiological conditions. Although this technique is commonly used on central nervous system structures such as spinal cord and brain, structures within the peripheral nervous system (including sensory ganglia and fibers) have proven to be much more difficult to study in acute preparations. Here we describe a method for patch-clamp recordings from rat dorsal root ganglion (DRG) slices.
authors.affiliation: Faculty of Biological Sciences, School of Biomedical Sciences, University of Leeds, Leeds, UK.
authors.forename: Kirstin E
authors.initials: KE
authors.lastname: Rose
authors.forename: Sylvain
authors.initials: S
authors.lastname: Gigout
authors.forename: Nikita
authors.initials: N
authors.lastname: Gamper
mesh_terms: D000818:Animals; D055724:Electrophysiological Phenomena; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology*; D008564:Membrane Potentials; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism; D051381:Rats
chemical_list: D024642:Potassium Channels, Voltage-Gated