title: Nano-sized 58S bioactive glass enhances proliferation and osteogenic genes expression of osteoblast-like cells.
abstract: OBJECTIVE
  To observe the effects of ionic dissolution products on nano-sized 58S bioactive glass (nano-58S) on proliferation and specific osteogenic genes expression in MG-63 cells.
  
  
  METHODS
  Ionic dissolution products were prepared by incubating nano-58S or sol-gel bioactive glass 58S (58S) particulates in Dulbecco's modified Eagle's medium (DMEM) at 1% w/v for 24 hr and filtrated through 0.22 µm filters to remove the particulates. MG-63 cells were cultured in the nano-58S extraction, 58S extraction, and DMEM, respectively, for different time periods to assay the proliferation, mRNA expression of alkaline phosphatase (ALP), Cbfa1, Collagen type I (Col-I) and osteocalcin (OCN), as well as ALP staining, activity and matrix mineralisation.
  
  
  RESULTS
  In the nano-58S group, cell proliferation and mRNA expression of ALP, Cbfa1 and OCN were significantly enhanced in a time-dependent manner compared with the control group. mRNA expression of Cbfa1 on day 4 and OCN on day 7 was significantly higher than that in the 58S group. Moreover, there was significantly more ALP protein expression and mineralisation in the nano-58S group than in the 58S group.
  
  
  CONCLUSIONS
  The nano-58S enhanced proliferation, osteogenic markers expression in MG-63 cells and induced stronger mineralization than 58S.
authors.affiliation: Department of Cariology and Endodontology, Peking University School, Beijing, People Republic of China.
authors.forename: Wei Yu
authors.initials: WY
authors.lastname: Gong
authors.forename: Yan Mei
authors.initials: YM
authors.lastname: Dong
authors.forename: Xiao Feng
authors.initials: XF
authors.lastname: Chen
authors.forename: Bekir
authors.initials: B
authors.lastname: Karabucak
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D002113:Calcification, Physiologic / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D024042:Collagen Type I / Q000032:analysis; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D003470:Culture Media; D005109:Extracellular Matrix / Q000648:ultrastructure; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology*; D012995:Solubility; D013997:Time Factors
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D003470:Culture Media; C448994:bioactive glass 58S; D015675:Osteocalcin; D000469:Alkaline Phosphatase