title: Linkage mapping and molecular diversity at the flower sex locus in wild and cultivated grapevine reveal a prominent SSR haplotype in hermaphrodite plants.
abstract: Cultivars used for wine and table grape have self-fertile hermaphrodite flowers whereas wild European vines and American and Asian species are dioecious, having either male or female flowers. Consistent with previous studies, the flower sex trait was mapped as a single major locus on chromosome 2 based on a pure Vitis vinifera population segregating for hermaphrodite and female progeny, and a hybrid population producing all three flower sex types. The sex locus was placed between the same SSR and SNP markers on both genetic maps, although abnormal segregation hampered to fine map the genomic region. From a total of 55 possible haplotypes inferred for three SSR markers around the sex locus, in a population of 132 V. sylvestris accessions and 171 V. vinifera cultivars, one of them accounted for 66 % of the hermaphrodite individuals and may be the result of domestication. Specific size variants of the VVIB23 microsatellite sequence within the 3'-UTR of a putative YABBY1 gene were found to be statistically significantly associated with the sex alleles M, H and f; these markers can provide assistance in defining the status of wild grapevine germplasm.
authors.affiliation: Department of Genomics and Biology of Fruit Crops, IASMA Research and Innovation Centre, Fondazione Edmund Mach Via E. Mach 1, 38010 San Michele all'Adige, TN, Italy.
authors.forename: Juri
authors.initials: J
authors.lastname: Battilana
authors.forename: Silvia
authors.initials: S
authors.lastname: Lorenzi
authors.forename: Flavia M
authors.initials: FM
authors.lastname: Moreira
authors.forename: Paula
authors.initials: P
authors.lastname: Moreno-Sanz
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Failla
authors.forename: Francesco
authors.initials: F
authors.lastname: Emanuelli
authors.forename: M Stella
authors.initials: MS
authors.lastname: Grando
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D032621:DNA Shuffling; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D035264:Flowers / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006239:Haplotypes / Q000235:genetics*; D057850:Hermaphroditic Organisms / Q000235:genetics*; D018895:Microsatellite Repeats; D027843:Vitis / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers