title: Increased β-oxidation with improved glucose uptake capacity in adipose tissue from obese after weight loss and maintenance.
abstract: OBJECTIVE
  We investigated protein markers for pathways of the fatty acids (FAs) and glucose metabolism in human adipose tissue after a weight loss program by calorie restriction.
  
  
  METHODS
  Overweight/obese subjects underwent an intervention of 5 weeks of a very low-calorie diet followed by a 3-week weight maintenance diet. Abdominal subcutaneous adipose tissue biopsies were sampled before and after the intervention. Seventeen target proteins as markers of metabolic pathways for the uptake and handling of FAs and glucose were quantified by Western blotting and 11 were retrieved from previous proteomics work. Correlation coefficients were calculated among changes of these proteins.
  
  
  RESULTS
  Short-chain 3-hydroxyacyl-CoA dehydrogenase, catalase, fatty acid translocase, fatty acid transporter protein 3, adipose triglyceride lipase, fatty acid-binding protein 4, aldolase-C, tubulin-β-5, and annexin A2 changed significantly, and lipoprotein lipase, perilipin 1, and hormone-sensitive lipase tended to change. On an average, increased glucose transporter type 4 translocation was observed, supported by a consistent increase of tyr-24 phosphorylated annexin A2.
  
  
  CONCLUSIONS
  Our findings suggest that after weight loss by calorie restriction and a short period of maintenance, adipose tissue has an increased capacity for glucose uptake, and lipid mobilization and oxidation. Such metabolic profile may relate to the health benefit of weight loss.
authors.affiliation: Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands.
authors.forename: Freek G
authors.initials: FG
authors.lastname: Bouwman
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Marleen
authors.initials: M
authors.lastname: van Baak
authors.forename: Wim H M
authors.initials: WH
authors.lastname: Saris
authors.forename: Edwin C M
authors.initials: EC
authors.lastname: Mariman
mesh_terms: D063988:3-Hydroxyacyl-CoA Dehydrogenase / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D017306:Annexin A2 / Q000378:metabolism; D031204:Caloric Restriction; D002352:Carrier Proteins / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D006801:Humans; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy / Q000378:metabolism*; D050177:Overweight / Q000178:diet therapy / Q000378:metabolism*; D010084:Oxidation-Reduction; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000378:metabolism; D002787:Sterol Esterase / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D015431:Weight Loss; D000217:Acyltransferases
chemical_list: D063988:3-Hydroxyacyl-CoA Dehydrogenase; D017306:Annexin A2; D002352:Carrier Proteins; D002374:Catalase; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose; D051275:Glucose Transporter Type 4; D008049:Lipase; D008071:Lipoprotein Lipase; D000070778:Perilipin-1; D010750:Phosphoproteins; D002787:Sterol Esterase; D014404:Tubulin; C472822:ANXA2 protein, human; C493410:FABP4 protein, human; C000602978:PLIN1 protein, human; C494794:PNPLA2 protein, human; D000217:Acyltransferases