title: Electrochemiluminescence energy transfer-promoted ultrasensitive immunoassay using near-infrared-emitting CdSeTe/CdS/ZnS quantum dots and gold nanorods.
abstract: The marriage of energy transfer with electrochemiluminescence has produced a new technology named electrochemiluminescence energy transfer (ECL-ET), which can realize effective and sensitive detection of biomolecules. To obtain optimal ECL-ET efficiency, perfect energy overlapped donor/acceptor pair is of great importance. Herein, we present a sensitive ECL-ET based immunosensor for the detection of tumor markers, using energy tunable CdSeTe/CdS/ZnS double shell quantum dots (QDs) and gold nanorods (GNRs) as the donor and acceptor, respectively. Firstly a facile microwave-assisted strategy for the synthesis of green- to near-infrared-emitting CdSeTe/CdS/ZnS QDs with time- and component-tunable photoluminescence was proposed. And, on the basis of the adjustable optical properties of both CdSeTe/CdS/ZnS QDs and GNRs, excellent overlap between donor emission and acceptor absorption can be obtained to ensure effective ECL-ET quenching, thus improving the sensing sensitivity. This method represents a novel approach for versatile detection of biomolecules at low concentrations.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Synergetic Innovation Center of Chemical Nature of Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Ji
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shen
authors.forename: Mi
authors.initials: M
authors.lastname: Xu
authors.forename: Rong
authors.initials: R
authors.lastname: Fei
authors.forename: Guohai
authors.initials: G
authors.lastname: Yang
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Zhang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D002104:Cadmium; D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D004735:Energy Transfer; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D043942:Nanotubes; D045663:Quantum Dots; D012643:Selenium; D013440:Sulfides; D013691:Tellurium; D017967:Zinc Compounds
chemical_list: D014408:Biomarkers, Tumor; D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D013440:Sulfides; D017967:Zinc Compounds; D002104:Cadmium; C034939:cadmium sulfide; D012643:Selenium; C031238:zinc sulfide; D013691:Tellurium