title: PCB 77 dechlorination products modulate pro-inflammatory events in vascular endothelial cells.
abstract: Persistent organic pollutants such as polychlorinated biphenyls (PCBs) are associated with detrimental health outcomes including cardiovascular diseases. Remediation of these compounds is a critical component of environmental policy. Although remediation efforts aim to completely remove toxicants, little is known about the effects of potential remediation byproducts. We previously published that Fe/Pd nanoparticles effectively dechlorinate PCB 77 to biphenyl, thus eliminating PCB-induced endothelial dysfunction using primary vascular endothelial cells. Herein, we analyzed the toxic effects of PCB congener mixtures (representative mixtures of commercial PCBs based on previous dechlorination data) produced at multiple time points during the dechlorination of PCB 77 to biphenyl. Compared with pure PCB 77, exposing endothelial cells to lower chlorinated PCB byproducts led to improved cellular viability, decreased superoxide production, and decreased nuclear factor kappa B activation based on duration of remediation. Presence of the parent compound, PCB 77, led to significant increases in mRNA and protein inflammatory marker expression. These data implicate that PCB dechlorination reduces biological toxicity to vascular endothelial cells.
authors.affiliation: University of Kentucky SRP Center, University of Kentucky, Lexington, KY, 40536-0200, USA.
authors.forename: Katryn
authors.initials: K
authors.lastname: Eske
authors.forename: Bradley
authors.initials: B
authors.lastname: Newsome
authors.forename: Sung Gu
authors.initials: SG
authors.lastname: Han
authors.forename: Margaret
authors.initials: M
authors.lastname: Murphy
authors.forename: Dibakar
authors.initials: D
authors.lastname: Bhattacharyya
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hennig
mesh_terms: D042783:Endothelial Cells; D015870:Gene Expression / Q000187:drug effects; D015386:Hazardous Substances / Q000633:toxicity*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*
chemical_list: D015386:Hazardous Substances; D011078:Polychlorinated Biphenyls; C028451:3,4,3',4'-tetrachlorobiphenyl