title: Dammarane-type saponins from heat-processed Gynostemma pentaphyllum show fortified activity against A549 cells.
abstract: An ethanol extract from heat-processed Gynostemma pentaphyllum showed more potent cytotoxic activity against human lung adenocarcinoma A549 cells than that of raw G. pentaphyllum. Four constituents were isolated from heat-processed G. pentaphyllum using resin HP-20, silica gel and reversed ODS column chromatography. They were identified by mass and NMR spectra as damulin A and damulin B, gypenoside L and gypenoside LI, respectively. To evaluate the efficacy of these four constituents, the MTT cytotoxicity assay was performed using A549 cells. Based on the structure of these four constituents, the results indicate that the hydroxyl group in C-2 and double bond in C20(21) and C20(22) positions are of importance in inhibition of A549 cell proliferation.
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, No. 27, Zhongguancun South Street, Haidian District, Beijing, 100081, China. xlpiao@163.com
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Piao
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Park
authors.forename: Dao-Jin
authors.initials: DJ
authors.lastname: Chen
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D029774:Gynostemma; D006358:Hot Temperature; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D000097669:Dammaranes
chemical_list: D012503:Saponins; D014315:Triterpenes; D000097669:Dammaranes