title: Quantitative proteomics of tomato defense against Pseudomonas syringae infection.
abstract: Genetic and microarray analyses have provided useful information in the area of plant and pathogen interactions. Pseudomonas syringae pv. tomato DC3000 (Pst) causes bacterial speck disease in tomato. Previous studies have shown that changes in response to pathogen infection at transcript level are variable at different time points. This study provides information not only on proteomic changes between a resistant and a susceptible genotype, but also information on changes between an early and a late time point. Using the iTRAQ quantitative proteomics approach, we have identified 2369 proteins in tomato leaves, and 477 of them were determined to be responsive to Pst inoculation. Unique and differential proteins after each comparison were further analyzed to provide information about protein changes and the potential functions they play in the pathogen response. This information is applicable not only to tomato proteomics, but also adds to the repertoire of proteins now available for crop proteomic analysis and how they change in response to pathogen infection.
authors.affiliation: Plant Molecular and Cellular Biology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Parker
authors.forename: Jin
authors.initials: J
authors.lastname: Koh
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Yoo
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.forename: Michelle
authors.initials: M
authors.lastname: Feole
authors.forename: Sarah
authors.initials: S
authors.lastname: Yi
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D060467:Disease Resistance / Q000502:physiology; D005838:Genotype; D006861:Hydrogen Peroxide; D007553:Isotope Labeling; D018551:Solanum lycopersicum / Q000378:metabolism / Q000502:physiology; D010935:Plant Diseases; D018515:Plant Leaves / Q000737:chemistry / Q000382:microbiology / Q000502:physiology; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D044224:Pseudomonas syringae; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D020543:Proteome; D006861:Hydrogen Peroxide
keywords: Defense response; Plant proteomics; Pseudomonas; Tomato; iTRAQ