title: Crystallization and preliminary X-ray analysis of human leukocyte cell-derived chemotaxin 2 (LECT2).
abstract: Human leukocyte cell-derived chemotaxin 2 (LECT2) is a chemotactic factor for neutrophils that plays multifunctional roles in liver regeneration, regulation of neuritic development and proliferation of chondrocytes and osteoblasts. In addition, the C-terminal region of LECT2 belongs to the zinc metalloendopeptidase M23 (PF01551) family. Purified LECT2 was crystallized using the sitting-drop vapour-diffusion method at 293 K. Crystals of selenomethionine-substituted LECT2 that diffracted X-rays to 1.94 Å resolution were obtained using a reservoir solution consisting of 0.2 M ammonium sulfate, 0.1 M HEPES pH 7.5, 25%(w/v) PEG 8000. The crystal belonged to space group P2₁2₁2₁, with unit-cell parameters a=59.4, b=63.5, c=64.0 Å. The calculated Matthews coefficient (VM=2.10 Å3 Da(-1), solvent content 40%) indicates that the crystal consists of two molecules per asymmetric unit.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Hai
authors.initials: H
authors.lastname: Zheng
authors.forename: Takuya
authors.initials: T
authors.lastname: Miyakawa
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Sawano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamagoe
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics; D007962:Leukocytes / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012645:Selenomethionine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C103468:LECT2 protein, human; D011994:Recombinant Proteins; D012645:Selenomethionine
keywords: chemotactic activity; human leukocyte cell-derived chemotaxin 2 (LECT2); metalloendopeptidase; protein refolding