title: Prostaglandin E2-induced cell death is mediated by activation of EP2 receptors in motor neuron-like NSC-34 cells.
abstract: Prostaglandin E2 (PGE2) was shown to induce neuronal death in the CNS. To characterize the neurotoxicity of PGE2 and E-prostanoid receptors (EP) in motor neurons, we investigated PGE2-induced cell death and the type(s) of EP responsible for mediating it in NSC-34, a motor neuron-like cell line. Immunoblotting studies showed that EP2 and EP3 were dominantly expressed in NSC-34 cells and motor neurons in mice. Exposure to PGE2 and butaprost, an EP2 agonist, but not sulprostone, an EP1/3 agonist, resulted in decreased viability of these cells. These results suggest that PGE2 induces cell death by activation of EP2 in NSC-34 cells.
authors.affiliation: Laboratory of Pharmacology, School of Pharmacy, Nihon University, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Miyagishi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kosuge
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoneoka
authors.forename: Maiko
authors.initials: M
authors.lastname: Ozone
authors.forename: Manami
authors.initials: M
authors.lastname: Endo
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Osada
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ishige
authors.forename: Kuniko
authors.initials: K
authors.lastname: Kusama-Eguchi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000209:etiology / Q000235:genetics*; D000818:Animals; D016923:Cell Death / Q000187:drug effects*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009046:Motor Neurons / Q000378:metabolism* / Q000473:pathology*; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000502:physiology*; D058308:Receptors, Prostaglandin E, EP3 Subtype / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology*
chemical_list: C545648:Ptger3 protein, mouse; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D015232:Dinoprostone