title: Pulsed electric field mediated in vitro cellular response of fibroblast and osteoblast-like cells on conducting austenitic stainless steel substrate.
abstract: This article reports the intermittent pulse electric field stimulus mediated in vitro cellular response of L929 mouse fibroblast/SaOS2 osteoblast-like cells on austenitic steel substrates in reference to the field strength dependent behavior. The cellular density and morphometric analyses revealed that the optimal electric (E) fields for the maximum cell density of adhered L929 (~270 % to that of untreated sample) and SaOS2 (~280 % to that of untreated sample) cells are 1 V (0.33 V/cm) and 2 V (0.67 V/cm), respectively. The trend in aspect ratio of elongated SaOS2 cells did not indicate any significant difference among the untreated and treated (up to 3.33 V/cm) cells. The average cell and nucleus areas (for SaOS2 cells) were increased with an increase in the applied voltage up to 8 V (2.67 V/cm) and reduced thereafter. However, the ratio of nucleus to total cell area was increased significantly on the application of higher voltages (2-10 V), indicating the possible influence of E-field on cell growth. Further, the cell density results were compared with earlier results obtained with sintered Hydroxyapatite (HA) and HA-BaTiO3 composites and such comparison revealed that the enhanced cell density on steel sample occurs upon application of much lower field strength and stimulation time. This indicates the possible role of substrate conductivity towards cell growth in pulsed E-field mediated culture conditions.
authors.affiliation: Laboratory for Biomaterials, Materials Research Center, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Ashutosh Kumar
authors.initials: AK
authors.lastname: Dubey
authors.forename: Parnika
authors.initials: P
authors.lastname: Agrawal
authors.forename: R Devesh Kumar
authors.initials: RD
authors.lastname: Misra
authors.forename: Bikramjit
authors.initials: B
authors.lastname: Basu
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004553:Electric Conductivity; D004558:Electric Stimulation / Q000379:methods; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D013193:Stainless Steel / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: C426023:austenitic steel; D013193:Stainless Steel