title: The structure of brazzein, a sweet-tasting protein from the wild African plant Pentadiplandra brazzeana.
abstract: Brazzein is the smallest sweet-tasting protein and was isolated from the wild African plant Pentadiplandra brazzeana. The brazzein molecule consists of 54 amino-acid residues and four disulfide bonds. Here, the first crystal structure of brazzein is reported at 1.8 Å resolution and is compared with previously reported solution structures. Despite the overall structural similarity, there are several remarkable differences between the crystal and solution structures both in their backbone folds and side-chain conformations. Firstly, there is an additional α-helix in the crystal structure. Secondly, the atomic r.m.s.d.s between the corresponding C(α)-atom pairs are as large as 2.0-2.2 Å between the crystal and solution structures. Thirdly, the crystal structure exhibits a molecular shape that is similar but not identical to the solution structures. The crystal structure of brazzein reported here will provide additional information and further insights into the intermolecular interaction of brazzein with the sweet-taste receptor.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nagata
authors.forename: Nobuko
authors.initials: N
authors.lastname: Hongo
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kameda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Woo Cheol
authors.initials: WC
authors.lastname: Lee
authors.forename: Kohki
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Ei-ichiro
authors.initials: E
authors.lastname: Suzuki
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D000349:Africa; D019607:Brassicaceae / Q000737:chemistry*; D018360:Crystallography, X-Ray; D010940:Plant Proteins / Q000737:chemistry*; D013549:Sweetening Agents / Q000737:chemistry*; D013649:Taste / Q000502:physiology
chemical_list: D010940:Plant Proteins; D013549:Sweetening Agents; C090521:brazzein protein, Pentadiplandra brazzeana
keywords: active sites; brazzein; crystal structure; solution structure; sweetness