title: Rapid determination of 5-hydroxymethylfurfural by DART ionization with time-of-flight mass spectrometry.
abstract: DART (direct analysis in real time), a novel technique with wide potential for rapid screening analysis, coupled with high-resolution time-of-flight mass spectrometry (TOF-MS) has been used for quantitative analysis of 5-hydroxymethylfurfural (5-HMF), a typical temperature marker of food. The DART/TOF-MS method was optimised and validated. Quantification of 5-HMF was achieved by use of a stable isotope-labelled 5-HMF standard prepared from glucose. Formation of 5-HMF from saccharides, a potential source of overestimation of results, was evaluated. Forty-four real samples (honey and caramelised condensed sweetened milk) and 50 model samples of heated honey were analysed. The possibility of using DART for analysis of heated samples of honey was confirmed. HPLC and DART/TOF-MS methods for determination of 5-HMF were compared. The correlation equation between these methods was DART = 1.0287HPLC + 0.21340, R(2) = 0.9557. The DART/TOF-MS method has been proved to enable efficient and rapid determination of 5-HMF in a variety of food matrices, for example honey and caramel.
authors.affiliation: Department of Food Preservation, Institute of Chemical Technology Prague, Prague 6, Czech Republic. ales.rajchl@vscht.cz
authors.forename: Aleš
authors.initials: A
authors.lastname: Rajchl
authors.forename: Ladislava
authors.initials: L
authors.lastname: Drgová
authors.forename: Adéla
authors.initials: A
authors.lastname: Grégrová
authors.forename: Helena
authors.initials: H
authors.lastname: Cížková
authors.forename: Rudolf
authors.initials: R
authors.lastname: Sevčík
authors.forename: Michal
authors.initials: M
authors.lastname: Voldřich
mesh_terms: D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*
chemical_list: C008046:5-hydroxymethylfurfural; D005662:Furaldehyde