title: Biosorption of copper and zinc by immobilised and free algal biomass, and the effects of metal biosorption on the growth and cellular structure of Chlorella sp. and Chlamydomonas sp. isolated from rivers in Penang, Malaysia.
abstract: In this study, the biosorption of copper and zinc ions by Chlorella sp. and Chlamydomonas sp. isolated from local environments in Malaysia was investigated in a batch system and by microscopic analyses. Under optimal biosorption conditions, the biosorption capacity of Chlorella sp. for copper and zinc ions was 33.4 and 28.5 mg/g, respectively, after 6 hr of biosorption in an immobilised system. Batch experiments showed that the biosorption capacity of algal biomass immobilised in the form of sodium alginate beads was higher than that of the free biomass. Scanning electron microscopy and energy-dispersive X-ray spectroscopy analyses revealed that copper and zinc were mainly sorbed at the cell surface during biosorption. Exposure to 5 mg/L of copper and zinc affected both the chlorophyll content and cell count of the algal cells after the first 12 hr of contact time.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia. wmaznah@usm.my
authors.forename: W O Wan
authors.initials: WO
authors.lastname: Maznah
authors.forename: A T
authors.initials: AT
authors.lastname: Al-Fawwaz
authors.forename: Misni
authors.initials: M
authors.lastname: Surif
mesh_terms: D000327:Adsorption; D018533:Biomass; D049109:Cell Proliferation / Q000187:drug effects; D018914:Cells, Immobilized / Q000737:chemistry; D002696:Chlamydomonas / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D002708:Chlorella / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D002734:Chlorophyll / Q000032:analysis; D003300:Copper / Q000302:isolation & purification* / Q000633:toxicity; D008855:Microscopy, Electron, Scanning; D045483:Rivers / Q000382:microbiology; D013052:Spectrometry, X-Ray Emission; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D015032:Zinc / Q000302:isolation & purification* / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D003300:Copper; D015032:Zinc