title: Endothelial cell behavior inside myoblast sheets with different thickness.
abstract: Using a cell sheet stacking method, we developed an in vitro culture system in which green fluorescent protein expressing human umbilical vein endothelial cells (GFP-HUVECs) were cultured under human skeletal muscle myoblast (HSMM) sheets with different layer numbers. Our aim in developing this system was to examine the different endothelial behaviors in the cell sheet. During 96 h of incubation, in monolayer HSMM sheet, HUVECs quickly reached the top of the cell sheet and detached. In three-layered HSMM sheet, HUVECs also migrated to the top layer and formed island-shaped aggregates. In five-layered HSMM sheet, HUVECs migrated into the middle of the cell sheet and formed net-shaped aggregates. In seven-layered HSMM sheet, HUVECs migrated in the basal of the cell sheet and formed sparse net-shaped aggregates. The thickness of the HSMM sheet, which can be controlled by the layer number of the cell sheet, is therefore an important parameter that affects the migration time, encounters, localization, and morphology of HUVECs inside the HSMM sheet.
authors.affiliation: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan.
authors.forename: Trung Xuan
authors.initials: TX
authors.lastname: Ngo
authors.forename: Eiji
authors.initials: E
authors.lastname: Nagamori
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kino-oka
mesh_terms: D018929:Cell Culture Techniques; D002465:Cell Movement; D042783:Endothelial Cells / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D032446:Myoblasts / Q000502:physiology*; D009924:Organ Culture Techniques