title: Fully validated simultaneous determination of bisoprolol fumarate and hydrochlorothiazide in their dosage forms using different voltammetric, chromatographic, and spectrophotometric analytical methods.
abstract: Voltammetric, chromatographic, and spectrophotometric methods were developed for the simultaneous determination of bisoprolol fumarate (BIS) and hydrochlorothiazide (HCZ). Differential pulse and square wave voltammetry techniques were used to analyze BIS and HCZ simultaneously by measuring at about 1400 and 1100 mV, respectively. RP-HPLC was the second method for simultaneous analysis of the compounds. The mixture of BIS, HCZ, and moxifloxacin as an internal standard was separated on an RP Zorbax Eclipse XDB-C18 column (150 x 4.6 mm, id, 5 microm particle size) using acetonitrile-15 mM phosphate (25+75, v/v) mobile phase at a 1.0 mL/min flow rate. The third method was based on first derivative of the ratio-spectra method obtained from the measurements of the amplitudes at 246 and 257 nm for BIS and HCZ, respectively. All the proposed methods were effectively applied for the simultaneous determination of BIS and HCZ in tablet dosage forms without any time-consuming extraction, sample preparation, or derivatization procedures.
authors.affiliation: Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Tandogan, Ankara, Turkey.
authors.forename: Burcin
authors.initials: B
authors.lastname: Bozal
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gumustas
authors.forename: Burcu
authors.initials: B
authors.lastname: Dogan-Topal
authors.forename: Bengi
authors.initials: B
authors.lastname: Uslu
authors.forename: Sibel A
authors.initials: SA
authors.lastname: Ozkan
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D017298:Bisoprolol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006852:Hydrochlorothiazide / Q000032:analysis*; D013053:Spectrophotometry; D013607:Tablets
chemical_list: D000959:Antihypertensive Agents; D013607:Tablets; D006852:Hydrochlorothiazide; D017298:Bisoprolol