title: Preparation and characterization of galactosylated bovine serum albumin nanoparticles for liver-targeted delivery of oridonin.
abstract: In this study, galactosylated bovine serum albumin (GB), which could be developed for a liver targeting carrier was synthetized and it was identified by Fourier transform infrared (FT-IR) spectrometer. Oridonin loaded bovine serum albumin nanoparticle (ORI-BSA-NP) and oridonin loaded GB nanoparticle (ORI-GB-NP) were prepared and optimized by the desolvation technique. During the preparation of ORI-GB-NP, galactosamine was introduced to end-cap the free aldehyde groups on nanoparticles. The characteristics of ORI-GB-NP such as particle size, zeta potential, particle morphologie, entrapment efficiency and drug loading were evaluated. The nearly spherical nanoparticles, with a narrow size distribution below 200 nm, were negatively charged with zeta potential of about -30 mV. Meanwhile, differential scanning calorimetry (DSC) and X-ray diffraction confirmed the amorphous state of ORI in ORI-GB-NP. The in vitro drug release of ORI from ORI-GB-NP presented a biphasic pattern with an initial burst effect and consequently sustained release. These results implied that the nanoparticles possessed fine physicochemical characteristics and seemed to be a stable delivery system for poorly soluble oridonin.
authors.affiliation: College of Pharmacy, Shandong University, Jinan 250012, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Li
authors.forename: Dianrui
authors.initials: D
authors.lastname: Zhang
authors.forename: Hejian
authors.initials: H
authors.lastname: Guo
authors.forename: Leilei
authors.initials: L
authors.lastname: Hao
authors.forename: Dandan
authors.initials: D
authors.lastname: Zheng
authors.forename: Guangpu
authors.initials: G
authors.lastname: Liu
authors.forename: Jingyi
authors.initials: J
authors.lastname: Shen
authors.forename: Xiaona
authors.initials: X
authors.lastname: Tian
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D045786:Diterpenes, Kaurane / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D004355:Drug Stability; D005690:Galactose / Q000737:chemistry*; D008099:Liver / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D033362:Powder Diffraction; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane; D004337:Drug Carriers; C011959:oridonin; D012710:Serum Albumin, Bovine; D005690:Galactose