title: Isolation of new polyketide metabolites, linearolides A and B, from Streptomyces sp. RK95-74.
abstract: Although all Streptomyces strains are now thought to have 20-30 gene clusters for secondary metabolite biosynthesis, we cannot actually identify so many kinds of metabolites from one strain by conventional methods. Using Streptomyces sp. RK95-74, previously found as a cytotrienin producer, we searched new metabolites other than cytotrienin derivatives. Following the cultivation with new media and the peak-guided fractionation, we have found new compounds with new polyketide scaffold, named linearolides A and B.
authors.affiliation: Antibiotics Laboratory, Chemical Biology Core Facility, RIKEN ASI, Wako, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ueki
authors.forename: Naofumi
authors.initials: N
authors.lastname: Koshiro
authors.forename: Harumi
authors.initials: H
authors.lastname: Aono
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawatani
authors.forename: Masakazu
authors.initials: M
authors.lastname: Uramoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D052978:Disk Diffusion Antimicrobial Tests; D004926:Escherichia coli / Q000187:drug effects; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D008968:Molecular Conformation; D005810:Multigene Family; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D020298:U937 Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D061065:Polyketides; C583210:linearolide A