title: Species-genomic relationships among the tribasic diploid and polyploid Carthamus taxa based on physical mapping of active and inactive 18S-5.8S-26S and 5S ribosomal RNA gene families, and the two tandemly repeated DNA sequences.
abstract: In the genus Carthamus (2n=20, 22, 24, 44, 64; x=10, 11, 12), most of the homologues within and between the chromosome complements are difficult to be identified. In the present work, we used fluorescent in situ hybridisation (FISH) to determine the chromosome distribution of the two rRNA gene families, and the two isolated repeated DNA sequences in the 14 Carthamus taxa. The distinctive variability in the distribution, number and signal intensity of hybridisation sites for 18S-26S and 5S rDNA loci could generally distinguish the 14 Carthamus taxa. Active 18S-26S rDNA sites were generally associated with NOR loci on the nucleolar chromosomes. The two A genome taxa, C. glaucus ssp. anatolicus and C. boissieri with 2n=20, and the two botanical varieties of B genome C. tinctorius (2n=24) had diagnostic FISH patterns. The present results support the origin of C. tinctorius from C. palaestinus. FISH patterns of C. arborescens vis-à-vis the other taxa indicate a clear division of Carthamus taxa into two distinct lineages. Comparative distribution and intensity pattern of 18S-26S rDNA sites could distinguish each of the tetraploid and hexaploid taxa. The present results indicate that C. boissieri (2n=20) is one of the genome donors for C. lanatus and C. lanatus ssp. lanatus (2n=44), and C. lanatus is one of the progenitors for the hexaploid (2n=64) taxa. The association of pCtKpnI-2 repeated sequence with rRNA gene cluster (orphon) in 2-10 nucleolar and non-nucleolar chromosomes and the consistent occurrence of pCtKpnI-1 repeated sequence at the subtelomeric region in all the taxa analysed indicate some functional role of these sequences.
authors.affiliation: Laboratory of Cellular and Molecular Cytogenetics, Department of Botany, University of Delhi, Delhi 110007, India.
authors.forename: Renuka
authors.initials: R
authors.lastname: Agrawal
authors.forename: Hisashi
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Rajesh
authors.initials: R
authors.lastname: Tandon
authors.forename: Satyawada Rama
authors.initials: SR
authors.lastname: Rao
authors.forename: Soom Nath
authors.initials: SN
authors.lastname: Raina
mesh_terms: D031178:Carthamus / Q000145:classification* / Q000235:genetics*; D032461:Chromosomes, Plant; D004171:Diploidy; D011123:Polyploidy; D018749:RNA, Plant; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; D020080:Tandem Repeat Sequences
chemical_list: D018749:RNA, Plant; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S