title: Mycetocola zhadangensis sp. nov., isolated from snow.
abstract: A Gram-stain-positive, aerobic, short rod-shaped bacterium, strain ZD1-4(T), was isolated from the Zhadang Glacier snow pit. The 16S rRNA gene sequence of the isolate showed highest similarity (98.8%) to that of Mycetocola manganoxydans MB1-14(T). The major fatty acids of strain ZD1-4(T) were anteiso-C(15 : 0), C(16 : 0), C(18 : 0) and anteiso-C(17:0). It possessed diphosphatidylglycerol as one of the major polar lipids, and MK-10 and MK-11 as the predominant isoprenoid quinones. The DNA G+C content of strain ZD1-4(T) was 63.8 ± 0.2 mol% (T(m)). A number of phenotypic characteristics distinguished this bacterium from the type strains of other species of the genus Mycetocola. Moreover, the novel isolate showed only approximately 50% DNA-DNA relatedness with M. manganoxydans MB1-14(T). According to these genotypic and phenotypic data, it is evident that strain ZD1-4(T) represents a novel species of the genus Mycetocola, for which the name Mycetocola zhadangensis sp. nov. is proposed. The type strain is ZD1-4(T) ( =KACC 16570(T) =CGMCC 1.12042(T)).
authors.affiliation: Key Laboratory of Tibetan Environmental Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100085, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Tibetan Environmental Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100085, PR China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tibetan Environmental Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100085, PR China.
authors.forename: Tandong
authors.initials: T
authors.lastname: Yao
authors.affiliation: Key Laboratory of Tibetan Environmental Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100085, PR China.
authors.forename: Shichang
authors.initials: S
authors.lastname: Kang
authors.affiliation: Key Laboratory of Microbial Engineering, The Institute of Biology, Henan Academy of Sciences, Zhengzhou, 450008, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, PR China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
authors.affiliation: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Tibetan Environmental Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100085, PR China.
authors.forename: Baiqing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory of Tibetan Environmental Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100085, PR China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Liu
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D046448:Ice Cover / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012914:Snow / Q000382:microbiology*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2