title: Production of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W.Curt. :Fr.) P. Karst. (higher Basidiomycetes), biomass and polysaccharides by solid state cultivation.
abstract: Solid state cultivation of Ganoderma lucidum biomass, strain BFWS Gal 4, originally isolated from the Slovenian forest, was studied in a horizontal stirred tank reactor. Periodic mixing of N = 80 rpm, 2 min/day was used. Production of fungal polysaccharides and fungal biomass on solid substrate based on beech sawdust, olive oil, and mineral salts was studied. Optimal moisture of the solid matrix was in the range of 80% to 74%. When the moisture content dropped below 57%, the growth of the mycelium and polysaccharide production stopped, but it revived when wet air was applied in further processing. Final concentration of biomass was 0.68 mg/g of solid substrate, while proportions of extracellular and intracellular polysaccharides were 4.5 mg/g and 1.05 mg/g, respectively.
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana; and National Institute of Chemistry, Ljubljana, Slovenia. marin.berovic@fkkt.uni-lj.si
authors.forename: Marin
authors.initials: M
authors.lastname: Berovic
authors.forename: Jozica
authors.initials: J
authors.lastname: Habijanic
authors.forename: Bojana
authors.initials: B
authors.lastname: Boh
authors.forename: Branka
authors.initials: B
authors.lastname: Wraber
authors.forename: Vlatka
authors.initials: V
authors.lastname: Petravic-Tominac
mesh_terms: D018533:Biomass; D019149:Bioreactors; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D008828:Microbiological Techniques; D020940:Reishi / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D062610:Fungal Polysaccharides