title: A pH gated, glucose-sensitive nanoparticle based on worm-like mesoporous silica for controlled insulin release.
abstract: This study prepares a kind of core-shell hybrid nanoparticles, which is worm-like, pH gated, and glucose-sensitive. It has a mesoporous silica nanoparticle (MSN) core and polymer shell (cross-linked and non-cross-linked), bearing 3-acrylamidophenylboronic acid (AAPBA) and N-isopropylacrylamide (NIPAM) as sensor moieties. The shell of the nanoparticles has presented a distinct transition from swollen state to collapsed state as the temperature increases, which offers easy access to drug loading. Here, insulin is applied as a model drug and the behaviors of its loading/release are investigated. Insulin loading is up to 15% via mesoporous silica core. In vitro experiment shows that the cumulative release of insulin is dependent on glucose concentration, and the glucose sensitivity could be adjusted simply by different pH values. Simultaneously, compared with the non-cross-linked shell, the cross-linked shell, using dextran-maleic acid (Dex-Ma) as a macromolecule cross-link, enables insulin to release more persistently. Also, cell viability assay indicates that these nanoparticles have good biocompatibility. Consequently, the novel, pH gated, glucose-sensitive core-shell nanoparticles may have potential applications as a vehicle of self-regulated insulin delivery system.
authors.affiliation: Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Xinge
authors.initials: X
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Zheng
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Wu
authors.forename: Chaoxing
authors.initials: C
authors.lastname: Li
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D004339:Drug Compounding; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry*; D007700:Kinetics; D008298:Maleates / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: C419826:3-acrylamidophenylboronic acid; D000178:Acrylamides; D001897:Boronic Acids; D003911:Dextrans; D004337:Drug Carriers; D007328:Insulin; D008298:Maleates; D012822:Silicon Dioxide; C030272:maleic acid; C067295:N-isopropylacrylamide; D005947:Glucose