title: Differential transport of Influenza A neuraminidase signal anchor peptides to the plasma membrane.
abstract: Influenza A Neuraminidase is essential for virus release from the cell surface of host cells. Given differential structures of the N-terminal sequences including the transmembrane domains of neuraminidase subtypes, we investigated their contribution to transport and localization of subtypes N1, N2 and N8 to the plasma membrane. We generated consensus sequences from all protein entries available for these subtypes. We found that 40N-terminal the forty N-terminal amino acids are sufficient to confer plasma membrane localization of fusion proteins, albeit with different efficiencies. Strikingly, subtle differences in the primary structure of the part of the transmembrane domain that resides in the exoplasmic leaflet of the membrane have a major impact on transport efficiency, providing a potential target for the inhibition of virus release.
authors.affiliation: Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
authors.forename: Andreas Max
authors.initials: AM
authors.lastname: Ernst
authors.forename: Sonja
authors.initials: S
authors.lastname: Zacherl
authors.forename: Alexia
authors.initials: A
authors.lastname: Herrmann
authors.forename: Moritz
authors.initials: M
authors.lastname: Hacke
authors.forename: Walter
authors.initials: W
authors.lastname: Nickel
authors.forename: Felix T
authors.initials: FT
authors.lastname: Wieland
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism*; D016384:Consensus Sequence; D006367:HeLa Cells; D006801:Humans; D009980:Influenza A virus / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry* / Q000378:metabolism*; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D021381:Protein Transport
chemical_list: D021382:Protein Sorting Signals; D009439:Neuraminidase