title: The PINK1-Parkin pathway promotes both mitophagy and selective respiratory chain turnover in vivo.
abstract: The accumulation of damaged mitochondria has been proposed as a key factor in aging and the pathogenesis of many common age-related diseases, including Parkinson disease (PD). Recently, in vitro studies of the PD-related proteins Parkin and PINK1 have found that these factors act in a common pathway to promote the selective autophagic degradation of damaged mitochondria (mitophagy). However, whether Parkin and PINK1 promote mitophagy under normal physiological conditions in vivo is unknown. To address this question, we used a proteomic approach in Drosophila to compare the rates of mitochondrial protein turnover in parkin mutants, PINK1 mutants, and control flies. We found that parkin null mutants showed a significant overall slowing of mitochondrial protein turnover, similar to but less severe than the slowing seen in autophagy-deficient Atg7 mutants, consistent with the model that Parkin acts upstream of Atg7 to promote mitophagy. By contrast, the turnover of many mitochondrial respiratory chain (RC) subunits showed greater impairment in parkin than Atg7 mutants, and RC turnover was also selectively impaired in PINK1 mutants. Our findings show that the PINK1-Parkin pathway promotes mitophagy in vivo and, unexpectedly, also promotes selective turnover of mitochondrial RC subunits. Failure to degrade damaged RC proteins could account for the RC deficits seen in many PD patients and may play an important role in PD pathogenesis.
authors.affiliation: Neurobiology and Behavior Program and Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.
authors.forename: Evelyn S
authors.initials: ES
authors.lastname: Vincow
authors.forename: Gennifer
authors.initials: G
authors.lastname: Merrihew
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Thomas
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Shulman
authors.forename: Richard P
authors.initials: RP
authors.lastname: Beyer
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
authors.forename: Leo J
authors.initials: LJ
authors.lastname: Pallanck
mesh_terms: D000818:Animals; D000071193:Autophagy-Related Protein 7; D001921:Brain / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology*; D006207:Half-Life; D007553:Isotope Labeling; D013058:Mass Spectrometry; D051379:Mice; D024101:Mitochondrial Proteins / Q000378:metabolism*; D063306:Mitophagy / Q000502:physiology*; D010300:Parkinson Disease / Q000209:etiology* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D000099202:PTEN-Induced Putative Kinase
chemical_list: D000071193:Autophagy-Related Protein 7; D029721:Drosophila Proteins; D024101:Mitochondrial Proteins; D017346:Protein Serine-Threonine Kinases; D044767:Ubiquitin-Protein Ligases; C530193:Atg7 protein, Drosophila; C534484:PINK1 protein, Drosophila; D000099202:PTEN-Induced Putative Kinase; C487430:park protein, Drosophila