title: Determination of carbohydrate-deficient transferrin in human serum by capillary zone electrophoresis: evaluation of assay performance and quality assurance over a 10-year period in the routine arena.
abstract: The performance of high-resolution CZE for determination of carbohydrate-deficient transferrin (CDT) in human serum based on internal and external quality data gathered over a 10-year period is reported. The assay comprises mixing of serum with a Fe(III) ion-containing solution prior to analysis of the iron saturated mixture in a dynamically double-coated capillary using a commercial buffer at alkaline pH. CDT values obtained with a human serum of a healthy individual and commercial quality control sera are shown to vary less than 10%. Values of a control from a specific lot were found to slowly decrease as function of time (less than 10% per year). Furthermore, due to unknown reasons, gradual changes in the monitored pattern around pentasialo-transferrin were detected, which limit the use of commercial control sera of the same lot to less than 2 years. Analysis of external quality control sera revealed correct classification of the samples over the entire 10-year period. Data obtained compare well with those of HPLC and CZE assays of other laboratories. The data gathered over a 10-year period demonstrate the robustness of the high-resolution CZE assay. This is the first account of a CZE-based CDT assay with complete internal and external quality assessment over an extended time period.
authors.affiliation: Clinical Pharmacology Laboratory, Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
authors.forename: Jeannine
authors.initials: J
authors.lastname: Joneli
authors.forename: Ursula
authors.initials: U
authors.lastname: Wanzenried
authors.forename: Jeannette
authors.initials: J
authors.lastname: Schiess
authors.forename: Christian
authors.initials: C
authors.lastname: Lanz
authors.forename: Jitka
authors.initials: J
authors.lastname: Caslavska
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Thormann
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D006801:Humans; D011786:Quality Control; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D014168:Transferrin; C056759:carbohydrate-deficient transferrin