title: Baicalin plays an anti-inflammatory role through reducing nuclear factor-κB and p38 phosphorylation in S. aureus-induced mastitis.
abstract: Mastitis is an inflammatory disease caused by microbial infection. Staphylococcus aureus is the major etiological microorganism responsible for both clinical and subclinical mastitis in dairy cows. A mouse model of S. aureus mastitis is available. Baicalin is isolated from Scutellaria and is known to have anti-inflammatory properties. This study was designed to evaluate the effects of baicalin in S. aureus mastitis. In the present study, the mouse model was infected with S. aureus to cause mammary gland inflammation. Baicalin treatment was administered from 6h until 24h after infection. Baicalin significantly attenuated inflammatory cell infiltration and decreased levels of TNF-α, IL-β, and IL-6. Further studies revealed that baicalin downregulated phosphorylation of NF-κB and p38 in the mammary gland with S. aureus mastitis. Our results demonstrated that baicalin reduced the expression of the proinflammatory cytokines TNF-α, IL-β, and IL-6 by inhibiting NF-κB and p38 phosphorylation and mRNA expression.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, People's Republic of China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Guo
authors.forename: Naisheng
authors.initials: N
authors.lastname: Zhang
authors.forename: Depeng
authors.initials: D
authors.lastname: Li
authors.forename: Dejie
authors.initials: D
authors.lastname: Liang
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Fenyang
authors.initials: F
authors.lastname: Li
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Fu
authors.forename: Yongguo
authors.initials: Y
authors.lastname: Cao
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008413:Mastitis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D013211:Staphylococcus aureus
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005419:Flavonoids; D016328:NF-kappa B; C038044:baicalin; D020928:Mitogen-Activated Protein Kinases