title: Salivary proteomic analysis and acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation.
abstract: Graft-versus-host disease (GVHD) is the major life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT), developing in 35%-70% of all allo-HSCT recipients despite immunosuppressive prophylaxis. The recent application of proteomic tools that allow screening for differentially expressed or excreted proteins in body fluids could possibly identify specific biomarkers for GVHD. Whole saliva is highly attractive for noninvasive specimen collection. In the present study, we collected saliva specimens from 40 consecutives patients who underwent allo-HSCT between December 2008 and March 2011 at our institution. The specimens were analyzed by HPLC coupled to electrospray-ionization mass spectrometry. Variable expression of S100 protein family members (S100A8, S100A9, and S100A7) was detected. Fourteen of 23 patients with GVHD demonstrated the presence of S100A8, compared with only 2 patients without GVHD and 0 patients in the control group (P = .001). S100A7 was detectable in 11 of the 23 patients with GVHD but was absent in the other 2 groups (P = .0001). S100A9-short was detected in 20 patients with GVHD, in 9 patients without GVHD, and in 8 healthy volunteers (P = .01) Further studies are needed to clarify the role of these proteins as a marker of GVHD or as an index of mucosal inflammation.
authors.affiliation: Hematology Department, Università Cattolica S. Cuore, Rome, Italy. p.chiusolo@rm.unicatt.it
authors.forename: Patrizia
authors.initials: P
authors.lastname: Chiusolo
authors.forename: Sabrina
authors.initials: S
authors.lastname: Giammarco
authors.forename: Chiara
authors.initials: C
authors.lastname: Fanali
authors.forename: Silvia
authors.initials: S
authors.lastname: Bellesi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Metafuni
authors.forename: Simona
authors.initials: S
authors.lastname: Sica
authors.forename: Federica
authors.initials: F
authors.lastname: Iavarone
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cabras
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Leone
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D040501:Calgranulin A / Q000235:genetics / Q000378:metabolism; D040502:Calgranulin B / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006086:Graft vs Host Disease / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism; D012463:Saliva / Q000737:chemistry*; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; D040501:Calgranulin A; D040502:Calgranulin B; D020543:Proteome; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human