title: Phylogenetic placement of two previously described intranuclear bacteria from the ciliate Paramecium bursaria (Protozoa, Ciliophora): 'Holospora acuminata' and 'Holospora curviuscula'.
abstract: 'Holospora acuminata' infects micronuclei of Paramecium bursaria (Protozoa, Ciliophora), whereas 'Holospora curviuscula' infects the macronucleus in other clones of the same host species. Because these micro-organisms have not been cultivated, their description has been based only on some morphological properties and host and nuclear specificities. One16S rRNA gene sequence of 'H. curviuscula' is present in databases. The systematic position of the representative strain of 'H. curviuscula', strain MC-3, was determined in this study. Moreover, for the first time, two strains of 'H. acuminata', KBN10-1 and AC61-10, were investigated. Phylogenetic analysis indicated that all three strains belonged to the genus Holospora, family Holosporaceae, order Rickettsiales within the Alphaproteobacteria.
authors.affiliation: Department of Genetics and Selection, Faculty of Biology and Soil Science, Saint Petersburg State University, 7-9 Universitetskaya nab., St. Petersburg 199034, Russia.
authors.forename: Maria S
authors.initials: MS
authors.lastname: Rautian
authors.affiliation: Department of Genetics and Selection, Faculty of Biology and Soil Science, Saint Petersburg State University, 7-9 Universitetskaya nab., St. Petersburg 199034, Russia.
authors.forename: Natalia D
authors.initials: ND
authors.lastname: Wackerow-Kouzova
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D042281:Holosporaceae / Q000145:classification* / Q000235:genetics; D048648:Macronucleus / Q000382:microbiology*; D008969:Molecular Sequence Data; D010247:Paramecium / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S