title: Association of calcium urolithiasis with urokinase P141L and 3'-UTR C>T polymorphisms in a Japanese population.
abstract: This was a case-control study to analyze the associations between calcium urolithiasis and the urokinase polymorphisms, P141L (rs2227564) and 3'-UTR C>T (rs4065), in a Japanese population. Cases consisted of 232 patients with urinary calcium stones (174 men and 58 women) who presented to a general hospital between April 2009 and June 2011. Among these cases, 115 (49.6 %) patients had calcium oxalate stones alone, and 113 (48.7 %) patients had calcium oxalate stones mixed with calcium phosphate stones. Controls consisted of 454 subjects who had a routine health check-up in the same prefecture. The two polymorphisms were genotyped via polymerase chain reaction with confronting two-pair primers. In the control group, the genotype frequencies of P141L were 0.573 for PP, 0.375 for PL, and 0.052 for LL, and those of 3'-UTR C>T were 0.835 for CC, 0.165 for CT, and TT was not identified. Neither of the polymorphisms was significantly associated with urolithiasis. The age- and sex-adjusted odds ratios of urolithiasis were 0.96 [95 % confidence interval (CI), 0.66-1.41] for PL and 1.22 (0.58-2.57) for LL as compared with PP genotype of P141L, and 1.01 (0.62-1.64) for CT as compared with CC genotype of 3'-UTR C>T. When compared with the PP genotype of P141L, the frequency of PL was significantly lower in female cases with a family history of urolithiasis than in females without such family history (p = 0.028). P141L and 3'-UTR polymorphisms of the urokinase gene are not associated with urolithiasis in a Japanese population.
authors.affiliation: Department of Urology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan. hagidesu@med.nagoya-u.ac.jp
authors.forename: Shoichi
authors.initials: S
authors.lastname: Hagikura
authors.forename: Kenji
authors.initials: K
authors.lastname: Wakai
authors.forename: Sayo
authors.initials: S
authors.lastname: Kawai
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Mariko
authors.initials: M
authors.lastname: Naito
authors.forename: Minako
authors.initials: M
authors.lastname: Hagikura
authors.forename: Momokazu
authors.initials: M
authors.lastname: Gotoh
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamajima
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D002129:Calcium Oxalate / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*; D052878:Urolithiasis / Q000235:genetics*
chemical_list: D002129:Calcium Oxalate; D014568:Urokinase-Type Plasminogen Activator