title: Subacute toxicity of cadmium on hepatocytes and nephrocytes in the rat could be considered as a green biosynthesis of nanoparticles.
abstract: The purpose was to study the toxicity of cadmium (Cd) and to explore its potential to generate nanoparticles during detoxification. In order to demonstrate this, in vivo fluorescence imaging, X-ray diffraction, and flow cytometry were performed. The in vivo imaging showed a fluorescence signal after Cd treatment (CdCl2, 1.50 mg/Kg, intraperitoneally). By contrast, the control-rat fluorescence was negative. The fluorescence was divided into three colors, red, yellow, and green, and probably indicates the presence of quantum dots. X-ray diffraction results revealed the presence of Cd sulfide (CdS) and/or Cd selenide (CdSe) nanoparticles following Cd injection in the liver (6.52 nm) and kidneys (56.30 nm). Interestingly, flow cytometry revealed a heterogeneous size distribution and a homogeneous granularity of synthesized nanoparticles. Using the green fluorescence channel and the red fluorescence channel, a narrow green emission spectrum and a broad red emission spectrum were detected, respectively, by cytometric analysis.
authors.affiliation: Laboratory of Physiologie Intégrée, Faculty of Sciences of Bizerte, Carthage University, Tunisia. hamditrabelsi@hotmail.com
authors.forename: Hamdi
authors.initials: H
authors.lastname: Trabelsi
authors.forename: Inès
authors.initials: I
authors.lastname: Azzouz
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.forename: Hafedh
authors.initials: H
authors.lastname: Abdelmelek
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D002478:Cells, Cultured; D005434:Flow Cytometry; D055772:Green Chemistry Technology / Q000379:methods*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D059551:Toxicity Tests, Subacute
chemical_list: D019187:Cadmium Compounds
keywords: XRD; cadmium selenide; cadmium sulfide; flow cytometry; in vivo imaging; quantum dots