title: Flow cytometry analysis of human fetal osteoblast fate processes on spark plasma sintered hydroxyapatite-titanium biocomposites.
abstract: Hydroxyapatite (HA)-based biocomposites have been widely investigated for a multitude of applications and these studies have been largely driven to improve mechanical properties (toughness and strength) without compromising cytocompatibility properties. Apart from routine cell viability/proliferation analysis, limited efforts have been made to quantify the fate processes (cell proliferation, cell cycle, and cell apoptosis) of human fetal osteoblast (hFOB) cells on HA-based composites, in vitro. In this work, the osteoblast cell fate process has been studied on a model hydroxyapatite-titanium (HA-Ti) system using the flow cytometry. In order to retain both HA and Ti, the novel processing technique, that is, spark plasma sintering, was suitably adopted. The cell fate processes of hFOBs, as evaluated using a flow cytometry, revealed statistically insignificant differences among HA-10 wt % Ti and HA and control (tissue culture polystyrene surface) in terms of osteoblast apoptosis, proliferation index as well as division index. For the first time, we provide quantified flow cytometry results to demonstrate that 10 wt % Ti additions to HA do not have any significant influence on the fate processes of human osteoblast-like cells, in vitro.
authors.affiliation: Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Kumar
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
authors.forename: Krishanu
authors.initials: K
authors.lastname: Biswas
authors.forename: Bikramjit
authors.initials: B
authors.lastname: Basu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D005333:Fetus / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D058626:Plasma Gases / Q000494:pharmacology*; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D058626:Plasma Gases; D017886:Durapatite; D014025:Titanium
keywords: HA-Ti; apoptosis; cell cycle; flow cytometry; proliferation