title: Sensitive and rapid detection of Trueperella pyogenes using loop-mediated isothermal amplification method.
abstract: Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Trueperella pyogenes DNA based on the plo gene sequence that encodes pyolysin. The results showed that target DNA was amplified specifically and with a detection limit 100-fold greater than PCR.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. wlzh2006@126.com
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiangli
authors.initials: X
authors.lastname: Meng
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000191:Actinomycetaceae / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006460:Hemolysin Proteins / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C108448:Pyolysin, Arcanobacterium pyogenes