title: [Advanced oxidation protein products inhibit proliferation and differentiation of rat osteoblasts through oxidative stress].
abstract: OBJECTIVE
  To investigate the effect of advanced oxidation protein products (AOPP) on proliferation, differentiation, and oxidative stress in rat osteoblasts.
  
  
  METHODS
  The cell proliferation and differentiation of osteoblasts isolated from neonatal SD rat skull were evaluated following treatment with different concentrations (50, 100, 200, and 400 µg/ml) of AOPP using CCK-8 kit and ALP assay kit, respectively. The levels of reactive oxygen species (ROS) in the treated cells were analyzed using 2', 7'-dichlorofluorescin diacetate, and the transcription levels of ALP, collagen I and RAGE were assessed using real-time PCR.
  
  
  RESULTS
  Compared with the control group, AOPP-treated osteoblasts showed obviously inhibited proliferation and differentiation with down-regulated expressions of ALP and collagen I and increased ROS production and RAGE expression.
  
  
  CONCLUSIONS
  AOPP can inhibit the proliferation and differentiation of rat osteoblasts partially by up-regulating RAGE and inducing ROS production.
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. sunnan0418@gmail.com
authors.forename: Nan
authors.initials: N
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Li
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Cheng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Dehong
authors.initials: D
authors.lastname: Cai
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism
chemical_list: D062107:Advanced Oxidation Protein Products; D024042:Collagen Type I; D017382:Reactive Oxygen Species; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D000469:Alkaline Phosphatase