title: Dynamics of biofilm formation under different nutrient levels and the effect on biofouling of a reverse osmosis membrane system.
abstract: Pseudomonas aeruginosa PAO1 wild type and a mucoid derivative (FRD1) which over produces alginate were used to foul reverse osmosis (RO) membranes. When operated at a constant flux, biofilm formation on the RO membrane resulted in a slow rise in transmembrane pressure (TMP) of 22% for the initial four days of operation, followed by a sharp increase of 159% over the following two days. The initial slow increase in TMP was probably due to the formation of a biofilm on the membrane surface, which then accelerated the rate of biofouling through the effect of concentration polarization. At later stages of operation, most of the bacterial biomass consisted of dead cells. The amount of extracellular polymeric substances appeared to correlate positively with the number of dead cells. The results indicate that prolonging the initial stage of slow TMP increase and avoiding the latter stage of accelerated TMP increase would provide a sustainable operation of the RO system. These results suggest that nutrient limitation could reduce biofilm accumulation and delay the increase in TMP.
authors.affiliation: Advanced Environmental Biotechnology Centre-Nanyang Environment & Water Research Institute (AEBC-NEWRI), Nanyang Technological University (NTU), Singapore.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Stanislaus Raditya
authors.initials: SR
authors.lastname: Suwarno
authors.forename: Tzyy Haur
authors.initials: TH
authors.lastname: Chong
authors.forename: Diane
authors.initials: D
authors.lastname: McDougald
authors.forename: Staffan
authors.initials: S
authors.lastname: Kjelleberg
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Cohen
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Fane
authors.forename: Scott A
authors.initials: SA
authors.lastname: Rice
mesh_terms: D000464:Alginates / Q000378:metabolism; D058491:Bacterial Load; D018441:Biofilms; D057219:Biofouling / Q000517:prevention & control*; D019149:Bioreactors; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008567:Membranes, Artificial; D050296:Microbial Viability; D009995:Osmosis; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011312:Pressure; D011550:Pseudomonas aeruginosa / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D011135:Polysaccharides, Bacterial; D020723:Glucuronic Acid