title: Phellinus linteus mushroom protects against tacrine-induced mitochondrial impairment and oxidative stress in HepG2 cells.
abstract: Tacrine (THA) was the first drug licensed for the treatment of Alzheimer's disease. Unfortunately, reversible hepatotoxicity is evident in about 30% of patients and limits its clinical use. The intracellular mechanisms have not yet been elucidated. Phellinus linteus (PL) is a mushroom that has long been used as a folk medicine. In our previous study, we found that PL could decrease the reactive oxygen species (ROS) formation in HepG2 cells. Presently, protective effects of PL on tacrine-induced ROS formation and mitochondria dysfunction were evaluated. The results showed that PL significantly reduced tacrine-induced ROS production, disruption of ΔΨm, 8-OHdG formation in mitochondrial DNA, and cytotoxicity in HepG2 cells. These data suggest that PL could attenuate the cytotoxicity and mitochondria dysfunction induced by tacrine in HepG2 cells. The protection is probably mediated by an antioxidant protective mechanism. Consumption of PL may be a plausible way to prevent tacrine-induced hepatotoxicity.
authors.affiliation: Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China.
authors.forename: Chunpeng
authors.initials: C
authors.lastname: Gao
authors.forename: Laifu
authors.initials: L
authors.lastname: Zhong
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.forename: Chengyan
authors.initials: C
authors.lastname: Geng
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000235:genetics; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D008518:Medicine, East Asian Traditional; D008519:Medicine, Traditional; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018697:Nootropic Agents / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D000084682:Phellinus; D010936:Plant Extracts; D011134:Polysaccharides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013619:Tacrine / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D004272:DNA, Mitochondrial; D018697:Nootropic Agents; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D013619:Tacrine