title: A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency.
abstract: The low toxicity and efficient gene delivery of polymeric vectors remain the major barrier to the clinical application of non-viral gene therapy. Here, we present a poly-D, L-succinimide (PSI)-based biodegradable cationic polymer which mimicked the golden standard, branched polyethylenimine (PEI, ~25 kDa). To investigate the influence of 1°, 2°, 3° amine group ratio in the polymer, a series of PSI-based vectors (PSI-NN'x-NNy) grafted with different amine side chains of N,N-dimethyldipropylenetriamine (NN') and bis(3-aminopropyl)amine (NN) were first characterized and contrasted by biophysical measurements. The in vitro and in vivo biological assay demonstrated that PSI-NN'0.85-NN1 exhibited better transfection ability and biocompatibility than PEI. The present results suggest that such PEI-mimic biodegradable PSI-NN'0.85-NN1 possesses a good potential application for clinical gene delivery.
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
authors.forename: J
authors.initials: J
authors.lastname: Shen
authors.forename: D J
authors.initials: DJ
authors.lastname: Zhao
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: Q L
authors.initials: QL
authors.lastname: Hu
authors.forename: Q W
authors.initials: QW
authors.lastname: Wang
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
authors.forename: G P
authors.initials: GP
authors.lastname: Tang
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D001673:Biodegradation, Environmental; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D004705:Endocytosis; D005434:Flow Cytometry; D005453:Fluorescence; D005822:Genetic Vectors / Q000378:metabolism*; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D009928:Organ Specificity; D010316:Particle Size; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry*; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000138:chemical synthesis* / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D000588:Amines; D001224:Aspartic Acid; D004247:DNA; D008156:Luciferases; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; C025252:poly-DL-succinimide