title: Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activities.
abstract: Paraoxonase 1 (PON1) is associated with HDL and modulates the antioxidant and anti-inflammatory role of HDL. The goals of the present study were to investigate the effect of ageing and the role of PON1 on the anti-inflammatory activity of HDL, and to determine whether extra-virgin olive oil (EVOO) consumption could improve the atheroprotective activity of HDL. HDL and PON1 were isolated from the plasma of ten young (Y-HDL and Y-PON1) and ten elderly (E-HDL and E-PON1) healthy volunteers before and after 12 weeks of EVOO consumption. Inflammation was assessed by measuring intracellular adhesion molecule 1 (ICAM-1) expression. THP-1 (human acute monocytic leukaemia cell line) monocyte chemotaxis was measured using a Boyden chamber. Oxidative damage to HDL was assessed by measuring conjugated diene formation and changes in electrophoretic migration. Y-HDL had more anti-inflammatory activity than E-HDL. The conjugated diene content and the electrophoretic mobility of E-HDL were higher than those of Y-HDL. Y-PON1 had significant anti-inflammatory activity, reducing ICAM-1 expression by 32·64 (SD 2·63)%, while E-PON1 had no significant effect. THP-1 chemotaxis measurements confirmed the ICAM-1 expression results. The 12 weeks of EVOO consumption significantly increased the anti-inflammatory activities of both HDL and PON1. The anti-inflammatory activity of HDL was modulated by PON1 and was lower in the elderly volunteers. EVOO consumption increased the anti-inflammatory effect of HDL and reduced the age-related decrease in anti-atherogenic activity.
authors.affiliation: Research Centre on Aging, Campus-Fleurimont, 3001, 12e Avenue Nord, Sherbrooke, QC, Canada J1H 4N4.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Loued
authors.forename: Hicham
authors.initials: H
authors.lastname: Berrougui
authors.forename: Pamela
authors.initials: P
authors.lastname: Componova
authors.forename: Souad
authors.initials: S
authors.lastname: Ikhlef
authors.forename: Olfa
authors.initials: O
authors.lastname: Helal
authors.forename: Abdelouahed
authors.initials: A
authors.lastname: Khalil
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D002633:Chemotaxis; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008297:Male; D008659:Metabolic Diseases / Q000517:prevention & control; D009000:Monocytes; D031658:Olea / Q000737:chemistry; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000494:pharmacology / Q000627:therapeutic use*; D055815:Young Adult
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004041:Dietary Fats; D008075:Lipoproteins, HDL; D000069463:Olive Oil; D010938:Plant Oils; D018799:Intercellular Adhesion Molecule-1; D043303:Aryldialkylphosphatase