title: Molecular cloning and characterization of amh, dax1 and cyp19a1a genes and their response to 17α-methyltestosterone in Pengze crucian carp.
abstract: The proteins encoded by amh, dax1 and cyp19a1a play important roles in gonad differentiation. Their functions have been far less studied in teleosts. In this study, the full-length cDNAs of amh, dax1 and cyp19a1a were cloned and characterized in a triploid gynogenic fish, the Pengze crucian carp. Their expression profilings in juvenile development, adult tissues and juveniles exposed to 100 ng/L 17α-methyltestosterone (MT) were investigated. Results showed that their putative proteins shared high identities to their counterparts in cyprinid fish species, respectively. The tissue distribution results indicated that amh and cyp19a1a were predominantly expressed in the ovary and dax1 was dominantly expressed in the liver. Gene profiling in the developmental stages showed that all the three target genes had a consistent highest expression at 48 days post hatching (dph). The period of 48 dph appeared to be a key time during the process of the gonad development of Pengze crucian carp. 100 ng/L MT significantly increased the mRNA expression of amh at 2- and 4-week exposures and enhanced dax1 and cyp19a1a at 6-week exposure. The present study indicated that MT could influence the gonad development in Pengze crucian carp by disturbing sex-differentiation associated gene expression. Furthermore, the present study will be of great significance to broaden the understanding of molecular mechanisms of the physiological processes of reproduction in fish.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Lihong
authors.initials: L
authors.lastname: Wang
authors.forename: Houpeng
authors.initials: H
authors.lastname: Wang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liang
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.forename: Fang
authors.initials: F
authors.lastname: Qin
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Liu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000378:metabolism*; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D002347:Carps / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003001:Cloning, Molecular; D057137:DAX-1 Orphan Nuclear Receptor / Q000235:genetics / Q000378:metabolism*; D004781:Environmental Exposure / Q000032:analysis; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008777:Methyltestosterone / Q000494:pharmacology*; D009928:Organ Specificity; D010053:Ovary / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000187:drug effects
chemical_list: D057137:DAX-1 Orphan Nuclear Receptor; D029941:Fish Proteins; D012333:RNA, Messenger; D054304:Anti-Mullerian Hormone; D001141:Aromatase; D008777:Methyltestosterone