title: A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.
abstract: We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.
authors.affiliation: Department of Photobiology and Bioimaging, The Scintillon Institute, San Diego, California, USA. nathanshaner@scintillon.org
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Shaner
authors.forename: Gerard G
authors.initials: GG
authors.lastname: Lambert
authors.forename: Andrew
authors.initials: A
authors.lastname: Chammas
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Ni
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Cranfill
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Baird
authors.forename: Brittney R
authors.initials: BR
authors.lastname: Sell
authors.forename: John R
authors.initials: JR
authors.lastname: Allen
authors.forename: Richard N
authors.initials: RN
authors.lastname: Day
authors.forename: Maria
authors.initials: M
authors.lastname: Israelsson
authors.forename: Michael W
authors.initials: MW
authors.lastname: Davidson
authors.forename: Jiwu
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D043344:Chordata / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D013269:Stochastic Processes
chemical_list: D049452:Green Fluorescent Proteins