title: In vivo mouse <formula>^{99m}</formula>Tc SPECT scans with bioluminescence imaging validation.
abstract: The aim of this in vivo study was to evaluate the feasibility of <formula>^{99m}</formula>Tc-labeled cartilage link protein (CLP) probe for the single-photon emission computed tomography (SPECT) of lung cancer. Xenograft mouse model were established from a luciferase expressing cell line derived from a human lung cancer. Bioluminescence imaging (BLI) was carried out prior to <formula>^{99m}</formula>Tc-CLP and <formula>^{99m}</formula>Tc-methoxyisobutyl isonitrile (MIBI) SPECT scans. The image quality of <formula>^{99m}</formula>Tc-CLP scan was validated with BLI and compared with well established <formula>^{99m}</formula>Tc-MIBI scan. Results of multimodal imaging analyses suggested that <formula>^{99m}</formula>Tc-CLP was a sensitive and reliable SPECT agent for lung cancer imaging.
authors.affiliation: Department of Experimental Nuclear Medicine, Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Liang
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Qiang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Liao
authors.forename: Xu-Sheng
authors.initials: XS
authors.lastname: Zhu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Luping
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000737:chemistry / Q000000981:diagnostic imaging / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*; D015609:Organotechnetium Compounds / Q000737:chemistry* / Q000493:pharmacokinetics; D011509:Proteoglycans / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D051598:Whole Body Imaging; D000099249:Proteoglycan Link Protein
chemical_list: D016326:Extracellular Matrix Proteins; D015609:Organotechnetium Compounds; D011509:Proteoglycans; D000099249:Proteoglycan Link Protein