title: Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion.
abstract: OBJECTIVE
  To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R).
  
  
  METHODS
  Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI): intestinal ischemia (60 min); Ischemia and Reperfusion (GIR): ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) : 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC): 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology.
  
  
  RESULTS
  Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC.
  
  
  CONCLUSIONS
  Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion.
authors.affiliation: UNIFESP, Sao Paulo, SP, Brazil. taha@uol.com.br
authors.forename: Murched Omar
authors.initials: MO
authors.lastname: Taha
authors.forename: Regiane Miranda
authors.initials: RM
authors.lastname: Ferreira
authors.forename: Nabiha Saadi Abrahão
authors.initials: NS
authors.lastname: Taha
authors.forename: Hugo Pequeno
authors.initials: HP
authors.lastname: Monteiro
authors.forename: Afonso
authors.initials: A
authors.lastname: Caricati-Neto
authors.forename: Itamar Souza
authors.initials: IS
authors.lastname: Oliveira-Júnior
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D042783:Endothelial Cells / Q000502:physiology*; D015870:Gene Expression / Q000235:genetics*; D007422:Intestines / Q000098:blood supply*; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000235:genetics* / Q000517:prevention & control; D015203:Reproducibility of Results; D013997:Time Factors