title: Knitted polylactide 96/4 L/D structures and scaffolds for tissue engineering: shelf life, in vitro and in vivo studies.
abstract: This study covers the whole production cycle, from biodegradable polymer processing to an in vivo tissue engineered construct. Six different biodegradable polylactide 96/4 L/D single jersey knits were manufactured using either four or eight multifilament fiber batches. The properties of those were studied in vitro for 42 weeks and in 0- to 3-year shelf life studies. Three types (Ø 12, 15 and 19 mm) of cylindrical scaffolds were manufactured from the knit, and the properties of those were studied in vitro for 48 weeks. For the Ø 15 mm scaffold type, mechanical properties were also studied in a one-year in vivo experiment. The scaffolds were implanted in the rat subcutis. All the scaffolds were γ-irradiated prior to the studies. In vitro, all the knits lost 99% of their mechanical strength in 30 weeks. In the three-year follow up of shelf life properties, there was no decrease in the mechanical properties due to the storage time and only a 12% decrease in molecular weight. The in vitro and in vivo scaffolds lost their mechanical properties after 1 week. In the case of the in vivo samples, the mechanical properties were restored again, stepwise, by the presence of growing/maturing tissue between weeks 3 and 12. Faster degradation was observed with in vitro scaffolds compared to in vivo scaffolds during the one-year follow up.
authors.affiliation: Department of Biomedical Engineering, Tampere University of Technology, Tampere, Finland. ville.ella@tut.fi
authors.forename: Ville
authors.initials: V
authors.lastname: Ellä
authors.forename: Tuija
authors.initials: T
authors.lastname: Annala
authors.forename: Satu
authors.initials: S
authors.lastname: Länsman
authors.forename: Manu
authors.initials: M
authors.lastname: Nurminen
authors.forename: Minna
authors.initials: M
authors.lastname: Kellomäki
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D051381:Rats; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; C033616:poly(lactide)
keywords: degradation; fibrous implant; in vitro; in vivo; knitting; polylactic acid; scaffold; shelf life; tissue engineering