title: The nonstructural protein NP1 of human bocavirus 1 induces cell cycle arrest and apoptosis in Hela cells.
abstract: Human bocavirus type 1 (HBoV1) is a newly identified pathogen associated with human respiratory tract illnesses. Previous studies demonstrated that proteins of HBoV1 failed to cause cell death, which is considered as a possible common feature of bocaviruses. However, our work showed that the NP1 of HBoV1 induced apoptotic cell death in Hela cells in the absence of viral genome replication and expression of other viral proteins. Mitochondria apoptotic pathway was involved in the NP1-induced apoptosis that was confirmed by apoptotic characteristics including morphological changes, DNA fragmentation and caspase activation. We also demonstrated that the cell cycle of NP1-transfected Hela cells was transiently arrested at G2/M phase followed by rapid appearance of apoptosis and that the N terminal domain of NP1 was critical to its nuclear localization and function in apoptosis induction in Hela cells. These findings might provide alternative information for further study of mechanism of HBoV1 pathogenesis.
authors.affiliation: College of Life Science, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Yingyue
authors.initials: Y
authors.lastname: Cai
authors.forename: Yongshu
authors.initials: Y
authors.lastname: Li
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D056661:Human bocavirus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D017361:Viral Nonstructural Proteins; D053148:Caspase 3; D053453:Caspase 9