title: Complete genome sequence of a Watermelon silver mottle virus isolate from China.
abstract: The complete genome of a Watermelon silver mottle virus (WSMoV) (genus Tospovirus, family Bunyaviridae) isolate (WSMoV-GZ) from Guangdong province, China was sequenced. The genomes of WSMoV-GZ contained 3,603, 4,909, and 8,914 nt of small (S), medium (M), and large (L) RNA segments, respectively, and had a genomic organization characteristic of members of the genus Tospovirus. The amino acid sequence of the nucleocapsid (N) protein, S RNA-encoded nonstructural (NSs) protein, M RNA-encoded nonstructural (NSm) protein, Gn/Gc glycoprotein precursor, and RNA-dependent RNA polymerase (RdRp) protein showed 94.3-97.5 % identity with those of other WSMoV isolates. Phylogenetic analysis showed that the N protein of WSMoV-GZ was clustered together with those of the WSMoV isolates. The full sequence of WSMoV-GZ provides a reference genome for comparison with other tospoviruses.
authors.affiliation: Laboratory of Plant Virology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China. xqrao@yahoo.com.cn
authors.forename: Xueqin
authors.initials: X
authors.lastname: Rao
authors.forename: Zhuyan
authors.initials: Z
authors.lastname: Wu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
mesh_terms: D002681:China; D029773:Citrullus / Q000821:virology; D016000:Cluster Analysis; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017865:Tospovirus / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins