title: Metabolomics for unknown plant metabolites.
abstract: In this article we discuss current trends in the techniques available for plant metabolomics. Chemical assignment of unknown metabolites leads to understanding of biosynthetic mechanisms at the gene level for genome-sequenced plants. Metabolomics using mass spectrometry has achieved innovative results in phytochemical genomics for primary and secondary metabolism in the model plant Arabidopsis thaliana by using publicly and commercially available information and standard compounds. However, finding a consolidated analytical technique for elucidation of structural information (e.g., elemental composition and structure) remains challenging. Recently, hyphenated analytical techniques and computer-assisted structural analysis with high-throughput and high-accuracy have been developing. Metabolite-driven approaches using such technology will be of central importance in phytochemical genomics.
authors.affiliation: RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakabayashi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D055432:Metabolomics / Q000379:methods / Q000639:trends*