title: Network balance via CRY signalling controls the Arabidopsis circadian clock over ambient temperatures.
abstract: Circadian clocks exhibit 'temperature compensation', meaning that they show only small changes in period over a broad temperature range. Several clock genes have been implicated in the temperature-dependent control of period in Arabidopsis. We show that blue light is essential for this, suggesting that the effects of light and temperature interact or converge upon common targets in the circadian clock. Our data demonstrate that two cryptochrome photoreceptors differentially control circadian period and sustain rhythmicity across the physiological temperature range. In order to test the hypothesis that the targets of light regulation are sufficient to mediate temperature compensation, we constructed a temperature-compensated clock model by adding passive temperature effects into only the light-sensitive processes in the model. Remarkably, this model was not only capable of full temperature compensation and consistent with mRNA profiles across a temperature range, but also predicted the temperature-dependent change in the level of LATE ELONGATED HYPOCOTYL, a key clock protein. Our analysis provides a systems-level understanding of period control in the plant circadian oscillator.
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool, UK.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Gould
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ugarte
authors.forename: Mirela
authors.initials: M
authors.lastname: Domijan
authors.forename: Maria
authors.initials: M
authors.lastname: Costa
authors.forename: Julia
authors.initials: J
authors.lastname: Foreman
authors.forename: Dana
authors.initials: D
authors.lastname: Macgregor
authors.forename: Ken
authors.initials: K
authors.lastname: Rose
authors.forename: Jayne
authors.initials: J
authors.lastname: Griffiths
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Millar
authors.forename: Bärbel
authors.initials: B
authors.lastname: Finkenstädt
authors.forename: Steven
authors.initials: S
authors.lastname: Penfield
authors.forename: David A
authors.initials: DA
authors.lastname: Rand
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Halliday
authors.forename: Anthony J W
authors.initials: AJ
authors.lastname: Hall
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D057906:Circadian Clocks; D056931:Cryptochromes / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008027:Light; D008954:Models, Biological; D008962:Models, Theoretical; D009154:Mutation; D030821:Plants, Genetically Modified; D015398:Signal Transduction; D013696:Temperature; D013816:Thermodynamics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; C103618:CRY2 protein, Arabidopsis; D056931:Cryptochromes; D004268:DNA-Binding Proteins; C113192:LHY protein, Arabidopsis; C411822:TOC1 protein, Arabidopsis; D014157:Transcription Factors