title: Production and characterization of monoclonal antibodies to Japanese encephalitis virus.
abstract: In this study, eighteen monoclonal antibodies (MAbs) to recent Japanese encephalitis virus (JEV) genotype I were produced and characterized by plaque reduction neutralization test, western blot analysis, indirect immunofluorescence assay and enzyme-linked immunosorbent assay. All MAbs recognized only envelope (E) protein or conformational epitope of E and precursor membrane proteins. Two MAbs (7E5 and 3-3H8) possessed virus-neutralization activity, and their escape mutants possessed a change of glutamine to histidine at the position of 52 of E protein, suggesting that these neutralizing MAbs recognize the domain I-II hinge region of E protein. Five MAbs recognized all examined flaviviruses, and two were specific to JEV. These MAbs may be useful for differentiation and diagnosis of flaviviruses.
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Hassan Y A H
authors.initials: HY
authors.lastname: Mahmoud
authors.forename: Keita
authors.initials: K
authors.lastname: Noguchi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Terada
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimojima
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D014759:Viral Envelope Proteins