title: An intercellular polyamine transfer via gap junctions regulates proliferation and response to stress in epithelial cells.
abstract: In the organism, quiescent epithelial cells have the potential to resume cycling as a result of various stimuli, including wound healing or oxidative stress. Because quiescent cells have a low polyamine level, resuming their growth requires an increase of their intracellular polyamine levels via de novo polyamine synthesis or their uptake from plasma. Another alternative, explored here, is an intercellular exchange with polyamine-rich cycling cells via gap junctions. We show that polyamines promote gap junction communication between proliferating cells by promoting dynamical microtubule plus ends at the cell periphery and thus allow polyamine exchange between cells. In this way, cycling cells favor regrowth in adjacent cells deprived of polyamines. In addition, intercellular interactions mediated by polyamines can coordinate the translational response to oxidative stress through the formation of stress granules. Some putative in vivo consequences of polyamine-mediated intercellular interactions are also discussed regarding cancer invasiveness and tissue regeneration.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, UMR829, Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, Université Evry-Val d'Essonne, Evry 91025, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Desforges
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Curmi
authors.forename: Ouissame
authors.initials: O
authors.lastname: Bounedjah
authors.forename: Samir
authors.initials: S
authors.lastname: Nakib
authors.forename: Loic
authors.initials: L
authors.lastname: Hamon
authors.forename: Jean-Pascal
authors.initials: JP
authors.lastname: De Bandt
authors.forename: David
authors.initials: D
authors.lastname: Pastré
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002450:Cell Communication; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques; D003514:Cyclohexylamines / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D017629:Gap Junctions / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors; D018384:Oxidative Stress; D011073:Polyamines / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism*; D051381:Rats; D013097:Spermine Synthase / Q000037:antagonists & inhibitors; D022502:Stress Fibers / Q000378:metabolism
chemical_list: D003514:Cyclohexylamines; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D013097:Spermine Synthase; D009955:Ornithine Decarboxylase; C081181:N-(3-aminopropyl)cyclohexylamine; D011700:Putrescine; D000518:Eflornithine