title: MicroRNA-155 is a novel suppressor of ovarian cancer-initiating cells that targets CLDN1.
abstract: Previous cDNA microarrays indicated that CLDN1 (claudin-1) is an important gene for ovarian cancer-initiating cell (OCIC) invasion and adhesion. Here, we show that the downregulation of miR-155 in OCICs correlates with CLDN1 overexpression and the suppression of OCIC invasion. Luciferase assays indicate that miR-155 targets CLDN1 mRNA on the 3' UTR. CLDN1 mRNA and claudin-1 protein expression were significantly decreased in miR-155-OCICs. Proliferation assays and Transwell migration assays show that miR-155 significantly suppresses the proliferative and invasive capacity of OCICs. Furthermore, miR-155 suppresses the growth of OCIC xenograft tumors. Thus, overexpression of miR-155 may prevent tumorigenesis in human ovarian cancer through downregulation of CLDN1.
authors.affiliation: Department of Medical Oncology, Shanghai Changzheng Hospital, Affiliated to The Second Military Medical University, Shanghai 200070, China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Qin
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Ren
authors.forename: Te
authors.initials: T
authors.lastname: Liu
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Huang
authors.forename: Jiejun
authors.initials: J
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D062445:Claudin-1 / Q000235:genetics*; D004285:Dogs; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C568108:CLDN1 protein, human; D015815:Cell Adhesion Molecules; D062445:Claudin-1; D018960:Hyaluronan Receptors; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D019009:Proto-Oncogene Proteins c-kit