title: Histophilus somni-induced infections in cattle from southern Brazil.
abstract: The sudden death of three calves, one diarrheic calf, and one aborted fetus from four farms in southern Brazil was investigated. Two Histophilus somni-associated syndromes were identified: systemic histophilosis (n = 4) and abortion (n = 1). The principal pathological findings included vasculitis, meningoencephalitis with thrombosis, necrotizing myocarditis, renal infarctions, hepatic abscesses, and bronchopneumonia. PCR assays were used to amplify specific amplicons of the ovine herpesvirus 2, bovine herpesvirus 1 and -5, Listeria monocytogenes, H. somni, and pestivirus; bovine group A rotavirus (BoRV-A) and bovine coronavirus (BCoV) were investigated in calves with diarrhea. H. somni DNA was amplified in tissues from all calves and the brain of the aborted fetus with pathological alterations consistent with histophilosis. All other PCR assays were negative; BoRV-A and BCoV were not identified. These findings confirm the participation of H. somni in the pathological alterations observed in this study and represent the first description of histophilosis in cattle from Brazil.
authors.affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine, Universidade Norte do Paraná, PR 218, Km 1, P.O Box 560, Arapongas, 86702-670, PR, Brazil, headleysa@gmail.com.
authors.forename: Selwyn A
authors.initials: SA
authors.lastname: Headley
authors.forename: Victor H S
authors.initials: VH
authors.lastname: Oliveira
authors.forename: Gustavo F
authors.initials: GF
authors.lastname: Figueira
authors.forename: Dalton E
authors.initials: DE
authors.lastname: Bronkhorst
authors.forename: Alice F
authors.initials: AF
authors.lastname: Alfieri
authors.forename: Werner
authors.initials: W
authors.lastname: Okano
authors.forename: Amauri A
authors.initials: AA
authors.lastname: Alfieri
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology / Q000473:pathology*; D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000473:pathology*; D006192:Haemophilus Infections / Q000150:complications / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D044138:Haemophilus somnus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008583:Meningitis, Haemophilus / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S