title: Fragmented adipose tissue graft for bone healing: histological and histometric study in rabbits' calvaria.
abstract: OBJECTIVE
  The adipose tissue represents an important reservoir of stem cells. There are few studies in the literature with which to histologically evaluate whether or not the adipose tissue graft is really a safe option to achieve bone repair. This study histologically analyzed the effect of fragmented autogenous adipose tissue grafts on bone healing in surgically created, critical-size defects (CSD) in a rabbit's calvaria.
  
  
  METHODS
  Forty-two New Zealand rabbits were used in this study. CSD that were 15 mm in diameter were created in the calvarium of each animal. The defects were randomly divided into two groups: in Group C (control), the defect was filled only by a blood clot and, in Group FAT (i.e., fragmented adipose tissue), the defect was filled with fragmented autogenous adipose tissue grafts. The groups were divided into subgroups (n = 7) for euthanasia at 7, 15, and 40 days after the procedure had been conducted. Histologic and histometric analyses were performed. Data were statistically analysed with ANOVA and Tukey's tests (p < 0.05).
  
  
  RESULTS
  The amount of bone formation did not show statistically significant differences seven days after the operation, which indicates that the groups had similar amounts of mineral deposition in the earlier period of the repair. Conversely, a significant of amount of bone matrix deposition was identified in the FAT group at 15 and 40 days following the operation, both on the border and in the body of the defect. Such an outcome was not found in the control group.
  
  
  CONCLUSIONS
  In this study, an autologous adipose tissue graft may be considered as likely biomaterial for bone regeneration, since it positively affected the amount of bone formation in surgically created CSD in the rabbits' calvaria 40 days after the procedure had been performed. Further investigations with a longer time evaluation are warranted to determine the effectiveness of autologous adipose tissue graft in the bone healing.
authors.affiliation: Master Program in Clinical Dentistry, Positivo University, Curitiba/PR, Brazil.
authors.forename: Lidiane-da Costa
authors.initials: LD
authors.lastname: Oliveira
authors.forename: Allan-Fernando
authors.initials: AF
authors.lastname: Giovanini
authors.forename: Allan
authors.initials: A
authors.lastname: Abuabara
authors.forename: Luiz-Gustavo
authors.initials: LG
authors.lastname: Klug
authors.forename: Carla-Castiglia
authors.initials: CC
authors.lastname: Gonzaga
authors.forename: João-Cézar
authors.initials: JC
authors.lastname: Zielak
authors.forename: Cícero-de Andrade
authors.initials: CD
authors.lastname: Urban
authors.forename: Tatiana-Miranda
authors.initials: TM
authors.lastname: Deliberador
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D008297:Male; D010012:Osteogenesis; D011817:Rabbits; D012886:Skull / Q000033:anatomy & histology / Q000601:surgery*; D014945:Wound Healing