title: Growth induction and low-oxygen apoptosis inhibition of human CD34+ progenitors in collagen gels.
abstract: Various reports have indicated low survival of injected progenitors into unfavorable environments such as the ischemic myocardium or lower limb tissues. This represents a major bottleneck in stem-cell-based cardiovascular regenerative medicine. Strategies to enhance survival of these cells in recipient tissues have been therefore sought to improve stem cell survival and ensure long-term engraftment. In the present contribution, we show that embedding human cord blood-derived CD34(+) cells into a collagen I-based hydrogel containing cytokines is a suitable strategy to promote stem cell proliferation and protect these cells from anoxia-induced apoptosis.
authors.affiliation: Laboratorio di Biologia Vascolare e Medicina Rigenerativa, Centro Cardiologico Monzino, 20138 Milano, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Avitabile
authors.forename: Katrin
authors.initials: K
authors.lastname: Salchert
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
authors.forename: Maurizio C
authors.initials: MC
authors.lastname: Capogrossi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pesce
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D017209:Apoptosis; D015687:Cell Hypoxia; D049109:Cell Proliferation; D002470:Cell Survival; D024042:Collagen Type I / Q000737:chemistry*; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D018952:Antigens, CD34; D024042:Collagen Type I; D020100:Hydrogels; D010100:Oxygen