title: Up-regulation of immunoglobulin G gene expression in the hippocampus of rats subjected to acute immobilization stress.
abstract: Immunoglobulin G (IgG) is thought to be produced by matured B lymphocytes, however, it was recently found to be synthesized in neurons of the brain, especially showing higher expression level in the hippocampus. To study the possible effects of IgG in the hippocampus, we examined IgG protein and mRNA expressions in rat hippocampal neurons with immunohistochemistry, immunofluorescence, in situ hybridization and laser microdissection-assisted RT-PCR. Increased IgG expressions at both protein and mRNA levels were detected in the hippocampus of an acute immobilization stress model of rat. No change was observed in the cortex or the thalamus. Furthermore, the microtubule-associated protein 2 (MAP2) and β III tubulin proteins did not show significant changes. Based on these findings, we hypothesize that hippocampal IgG may play a key role in adverse circumstances such as stress. The finding of increased IgG expression in the hippocampus following stress may also provide possibilities for developing antidepressant medication.
authors.affiliation: Provincial Key Laboratory of Infectious Diseases and Immunopathology, Department of Pathology and Pathophysiology, Shantou University Medical College, Shantou, Guangdong, China. shengwang.diamond@gmail.com
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Guowei
authors.initials: G
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Shuiqin
authors.initials: S
authors.lastname: Lin
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000276:immunology* / Q000378:metabolism; D007103:Immobilization; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D061346:Laser Capture Microdissection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological; D015854:Up-Regulation
chemical_list: D007074:Immunoglobulin G