title: High-throughput proteomic characterization of plasma rich in growth factors (PRGF-Endoret)-derived fibrin clot interactome.
abstract: Plasma rich in growth factors (PRGF®-Endoret®) is an autologous technology that contains a set of proteins specifically addressed to wound healing and tissue regeneration. The scaffold formed by using this technology is a clot mainly composed of fibrin protein, forming a three-dimensional (3D) macroscopic network. This biomaterial is easily obtained by biotechnological means from blood and can be used in a range of situations to help wound healing and tissue regeneration. Although the main constituent of this clot is the fibrin scaffold, little is known about other proteins interacting in this clot that may act as adjuvants in the healing process. The aim of this study was to characterize the proteins enclosed by PRGF-Endoret scaffold, using a double-proteomic approach that combines 1D-SDS-PAGE approach followed by LC-MS/MS, and 2-DE followed by MALDI-TOF/TOF. The results presented here provide a description of the catalogue of key proteins in close contact with the fibrin scaffold. The obtained lists of proteins were grouped into families and networks according to gene ontology. Taken together, an enrichment of both proteins and protein families specifically involved in tissue regeneration and wound healing has been found.
authors.affiliation: BTI-Biotechnology Institute, Vitoria, Spain.|Eduardo Anitua Foundation for Biomedical Research, Vitoria, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Anitua
authors.affiliation: BTI-Biotechnology Institute, Vitoria, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Prado
authors.affiliation: Proteomics Platform, CIC bioGUNE, ProteoRed, CIBER-ehd, Bizkaia Technology Park, Derio, Spain.
authors.forename: Mikel
authors.initials: M
authors.lastname: Azkargorta
authors.affiliation: Proteomics Platform, CIC bioGUNE, ProteoRed, CIBER-ehd, Bizkaia Technology Park, Derio, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Rodriguez-Suárez
authors.affiliation: Proteomics Platform, CIC bioGUNE, ProteoRed, CIBER-ehd, Bizkaia Technology Park, Derio, Spain.
authors.forename: Ibon
authors.initials: I
authors.lastname: Iloro
authors.affiliation: Centro Nacional de Biotecnología. Lab 115. Darwin 3, Campus de Cantoblanco, 28049, Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Casado-Vela
authors.affiliation: Proteomics Platform, CIC bioGUNE, ProteoRed, CIBER-ehd, Bizkaia Technology Park, Derio, Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Elortza
authors.affiliation: BTI-Biotechnology Institute, Vitoria, Spain.|Eduardo Anitua Foundation for Biomedical Research, Vitoria, Spain.
authors.forename: Gorka
authors.initials: G
authors.lastname: Orive
mesh_terms: D001709:Biotechnology; D001777:Blood Coagulation; D001792:Blood Platelets / Q000166:cytology; D001798:Blood Proteins / Q000737:chemistry*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005337:Fibrin / Q000737:chemistry*; D064368:Healthy Volunteers; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry*; D007962:Leukocytes / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D020543:Proteome / Q000737:chemistry*; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry; D014945:Wound Healing
chemical_list: D001798:Blood Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011506:Proteins; D020543:Proteome; D005337:Fibrin; D014357:Trypsin
keywords: fibrin clot; plasma rich in growth factors; platelet-rich plasma; proteomics; scaffold; tissue regeneration