title: Molecular serogrouping of Shiga toxin-producing Escherichia coli using suspension array.
abstract: A suspension array assay was developed for molecular serotyping of the seven most prevalent Shiga toxin-producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, O145, and O157). Fluorescence values of 59 STEC were 30 to >270 times greater than the signals of negative controls, demonstrating the method's effectiveness for the molecular serotyping of STEC.
authors.affiliation: Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Magaly
authors.initials: M
authors.lastname: Toro
authors.forename: Mohamed Badaoui
authors.initials: MB
authors.lastname: Najjar
authors.forename: Wenting
authors.initials: W
authors.lastname: Ju
authors.forename: Eric
authors.initials: E
authors.lastname: Brown
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.forename: Jianghong
authors.initials: J
authors.lastname: Meng
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D005516:Food Microbiology; D026901:Membrane Transport Proteins / Q000235:genetics; D008863:Microspheres; D060885:Multiplex Polymerase Chain Reaction; D009693:Nucleic Acid Hybridization; D012703:Serotyping; D022622:Shiga Toxin 1 / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C543926:Wzx protein, E coli