title: T-cell-derived, but not B-cell-derived, IL-10 suppresses antigen-specific T-cell responses in Litomosoides sigmodontis-infected mice.
abstract: IL-10, a cytokine with pleiotropic functions is produced by many different cells. Although IL-10 may be crucial for initiating protective Th2 responses to helminth infection, it may also function as a suppressive cytokine preventing immune pathology or even contributing to helminth-induced immune evasion. Here, we show that B cells and T cells produce IL-10 during murine Litomosoides sigmodontis infection. IL-10-deficient mice produced increased amounts of L. sigmodontis-specific IFN-γ and IL-13 suggesting a suppressive role for IL-10 in the initiation of the T-cell response to infection. Using cell type-specific IL-10-deficient mice, we dissected different functions of T-cell- and B-cell-derived IL-10. Litomosoides sigmodontis-specific IFN-γ, IL-5, and IL-13 production increased in the absence of T-cell-derived IL-10 at early and late time points of infection. In contrast, B-cell-specific IL-10 deficiency did not lead to significant changes in L. sigmodontis-specific cytokine production compared to WT mice. Our results suggest that the initiation of Ag-specific cellular responses during L. sigmodontis infection is suppressed by T-cell-derived IL-10 and not by B-cell-derived IL-10.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Irma
authors.initials: I
authors.lastname: Haben
authors.forename: Wiebke
authors.initials: W
authors.lastname: Hartmann
authors.forename: Sabine
authors.initials: S
authors.lastname: Specht
authors.forename: Achim
authors.initials: A
authors.lastname: Hoerauf
authors.forename: Axel
authors.initials: A
authors.lastname: Roers
authors.forename: Werner
authors.initials: W
authors.lastname: Müller
authors.forename: Minka
authors.initials: M
authors.lastname: Breloer
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D005368:Filariasis / Q000276:immunology*; D005370:Filarioidea / Q000276:immunology*; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: C556062:IL10 protein, mouse; D016753:Interleukin-10
keywords: IL-10; Th1/Th2; nematode