title: Effect of nanoparticles and environmental particles on a cocultures model of the air-blood barrier.
abstract: Exposure to engineered nanoparticles (NPs) and to ambient particles (PM) has increased significantly. During the last decades the application of nano-objects to daily-life goods and the emissions produced in highly urbanized cities have considerably augmented. As a consequence, the understanding of the possible effects of NPs and PM on human respiratory system and particularly on the air-blood barrier (ABB) has become of primary interest. The crosstalk between lung epithelial cells and underlying endothelial cells is indeed essential in determining the effects of inhaled particles. Here we report the effects of metal oxides NPs (CuO and TiO2) and of PM on an in vitro model of the ABB constituted by the type II epithelial cell line (NCI-H441) and the endothelial one (HPMEC-ST1.6R). The results demonstrate that apical exposure of alveolar cells induces significant modulation of proinflammatory proteins also in endothelial cells.
authors.affiliation: Department of Earth and Environmental Sciences, Research Centre Polaris, University of Milano Bicocca, Piazza della Scienza 1, 20126 Milano, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Bengalli
authors.forename: Paride
authors.initials: P
authors.lastname: Mantecca
authors.forename: Marina
authors.initials: M
authors.lastname: Camatini
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gualtieri
mesh_terms: D015824:Blood-Air Barrier / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D018920:Coculture Techniques; D017097:Electric Impedance; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007249:Inflammation; D008833:Microcirculation / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D052638:Particulate Matter / Q000737:chemistry; D011650:Pulmonary Alveoli / Q000187:drug effects
chemical_list: D010087:Oxides; D052638:Particulate Matter