title: The characterisation of Pax3 expressant cells in adult peripheral nerve.
abstract: Pax3 has numerous integral functions in embryonic tissue morphogenesis and knowledge of its complex function in cells of adult tissue continues to unfold. Across a variety of adult tissue lineages, the role of Pax3 is principally linked to maintenance of the tissue's resident stem/progenitor cell population. In adult peripheral nerves, Pax3 is reported to be expressed in nonmyelinating Schwann cells, however, little is known about the purpose of this expression. Based on the evidence of the role of Pax3 in other adult tissue stem and progenitor cells, it was hypothesised that the cells in adult peripheral nerve that express Pax3 may be peripheral glioblasts. Here, methods have been developed for identification and visualisation of Pax3 expressant cells in normal 60 day old mouse peripheral nerve that allowed morphological and phenotypic distinctions to be made between Pax3 expressing cells and other nonmyelinating Schwann cells. The distinctions described provide compelling support for a resident glioblast population in adult mouse peripheral nerve.
authors.affiliation: School of Medical Science, Edith Cowan University, Joondalup, Western Australia, Australia.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Blake
authors.forename: Melanie R
authors.initials: MR
authors.lastname: Ziman
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism*; D010641:Phenotype; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D020800:Receptor, Nerve Growth Factor; C068686:Pax3 protein, mouse