title: Endogenous plasma glucagon-like peptide-1 following acute dietary fibre consumption.
abstract: SCFA resulting from the microbial fermentation of carbohydrates have been linked to increased glucagon-like peptide-1 (GLP-1) secretion from the gastrointestinal tract in cell and animal models; however, there is little direct evidence in human subjects to confirm this. The present study was designed to investigate whether endogenous plasma GLP-1 concentrations increase following acute consumption of 48 g dietary fibre (as resistant starch (RS) from high-amylose maize type 2 RS (HAM-RS2)) compared with a matched placebo. A total of thirty healthy males participated in the present randomised cross-over study where HAM-RS2 or placebo was consumed as part of standardised breakfast and lunch meals. Changes to GLP-1, glucose, insulin and C-peptide were assessed half hourly for 7 h. Following the breakfast meal, plasma GLP-1 concentrations were lower with HAM-RS2 compared with the placebo (P = 0·025). However, there was no significant difference between the supplements following the lunch meal. Plasma insulin concentrations were significantly lower following the lunch meal (P = 0·034) with HAM-RS2 than with the placebo, but were not different after breakfast. Plasma glucose and C-peptide concentrations did not differ at any point. These results suggest that increased dietary fibre intake, in the form of HAM-RS2, does not acutely increase endogenous GLP-1 concentrations in human subjects. Further fibre feeding studies are required to determine whether GLP-1 concentrations may increase following longer-term consumption.
authors.affiliation: Department of Nutritional Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK.
authors.forename: Caroline L
authors.initials: CL
authors.lastname: Bodinham
authors.forename: Najlaa M
authors.initials: NM
authors.lastname: Al-Mana
authors.forename: Leanne
authors.initials: L
authors.lastname: Smith
authors.forename: M Denise
authors.initials: MD
authors.lastname: Robertson
mesh_terms: D000328:Adult; D019540:Area Under Curve; D002096:C-Peptide / Q000097:blood; D018592:Cross-Over Studies; D004032:Diet; D004043:Dietary Fiber / Q000008:administration & dosage*; D019587:Dietary Supplements; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology; D052216:Glucagon-Like Peptide 1 / Q000097:blood*; D006801:Humans; D054795:Incretins / Q000097:blood; D007328:Insulin / Q000097:blood; D008297:Male; D019518:Postprandial Period; D000084922:Resistant Starch; D016037:Single-Blind Method; D013213:Starch / Q000008:administration & dosage / Q000031:analogs & derivatives*; D055815:Young Adult
chemical_list: D002096:C-Peptide; D004043:Dietary Fiber; D054795:Incretins; D007328:Insulin; D000084922:Resistant Starch; C572111:high-amylose maize type 2 resistant starch, maize; D052216:Glucagon-Like Peptide 1; D013213:Starch