title: TaAbc1, a member of Abc1-like family involved in hypersensitive response against the stripe rust fungal pathogen in wheat.
abstract: To search for genes involved in wheat (Triticum aestivum L.) defense response to the infection of stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst), we identified and cloned a new wheat gene similar to the genes in the Abc1-like gene family. The new gene, designated as TaAbc1, encodes a 717-amino acid, 80.35 kD protein. The TaAbc1 protein contains two conserved domains shared by Abc1-like proteins, two trans-membrane domains at the C-terminal, and a 36-amino acid chloroplast targeting presequence at the N-terminal. Characterization of TaAbc1 expression revealed that gene expression was tissue-specific and could be up-regulated by biotic agents (e.g., stripe rust pathogen) and/or by an abiotic stress like wounding. High-fold induction was associated with the hypersensitive response (HR) triggered only by avirulent stripe rust pathotypes, suggesting that TaAbc1 is a rust-pathotype specific HR-mediator. Down-regulating TaAbc1 reduced HR but not the overall resistance level in Suwon11 to CYR23, suggesting TaAbc1 was involved in HR against stripe rust, but overall host resistance is not HR-dependent.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wang
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Duan
authors.forename: Shuining
authors.initials: S
authors.lastname: Yin
authors.forename: Hongchang
authors.initials: H
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D001487:Basidiomycota / Q000276:immunology*; D003001:Cloning, Molecular; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D034622:RNA Interference; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology
chemical_list: D018528:ATP-Binding Cassette Transporters