title: Neuroprotective effects of hypothermia and erythropoietin after perinatal asphyxia in newborn rats.
abstract: OBJECTIVE
  Evaluation of neuroprotective effects of hypothermia, erythropoietin and their simultaneous use after perinatal asphyxia in newborn rats.
  
  
  METHODS
  Hysterectomy was performed to Wistar female rats on the last day of gestation. Perinatal asphyxia was induced by submersion of uterus containing pups in saline for 15 min. After resuscitation, pups were randomized into 4 groups, 15 animals in each: G1 - asphyxia; G2 - asphyxia + hypothermia (rectal temperature 33 °C for 1 h); G3 - asphyxia + erythropoietin (Darbepoetin-α 2.5 μg, intraperitoneally) and G4 - asphyxia + erythropoietin + hypothermia. Pups were sacrificed on 7th day of life and histopathological analysis of hippocampus was performed.
  
  
  RESULTS
  Measure of damage to dorsal, ventral and entire hippocampus was significantly lower in groups G2, G3 and G4 than in group G1 (p ~ 0.00; respectively). Measure of damage to hippocampus in group G4 was significantly lower than in group G2 (p = 0.029).
  
  
  CONCLUSIONS
  This study demonstrates that simultaneous use of hypothermia and erythropoietin has more expressed neuroprotective effects than sole use of hypothermia after perinatal asphyxia in newborn rats.
authors.affiliation: Institute of Child and Youth Healthcare of Vojvodina , Novi Sad , Serbia .
authors.forename: Slobodan D
authors.initials: SD
authors.lastname: Spasojevic
authors.forename: Vesna D
authors.initials: VD
authors.lastname: Stojanovic
authors.forename: Nenad A
authors.initials: NA
authors.lastname: Barisic
authors.forename: Aleksandra R
authors.initials: AR
authors.lastname: Doronjski
authors.forename: Dragan R
authors.initials: DR
authors.lastname: Zikic
authors.forename: Sinisa M
authors.initials: SM
authors.lastname: Babovic
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001238:Asphyxia Neonatorum / Q000628:therapy*; D001927:Brain Diseases / Q000473:pathology / Q000517:prevention & control*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000008:administration & dosage*; D005260:Female; D006624:Hippocampus / Q000473:pathology; D007036:Hypothermia, Induced; D009474:Neurons / Q000473:pathology; D018696:Neuroprotective Agents; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018696:Neuroprotective Agents; D004921:Erythropoietin