title: Simple resazurin-based microplate assay for measuring Chlamydia infections.
abstract: The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measure Chlamydia infection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA.
authors.forename: Ichie
authors.initials: I
authors.lastname: Osaka
authors.forename: P Scott
authors.initials: PS
authors.lastname: Hefty
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002690:Chlamydia Infections / Q000188:drug therapy / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000187:drug effects*; D005347:Fibroblasts / Q000382:microbiology / Q000502:physiology; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism*; D007739:L Cells / Q000382:microbiology; D051379:Mice; D008826:Microbial Sensitivity Tests / Q000379:methods; D010078:Oxazines / Q000378:metabolism*; D011112:Polymyxin B / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D014966:Xanthenes / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D013752:Tetracycline; D011112:Polymyxin B