title: Co-expression of activin receptor-interacting protein 1 and 2 in mouse nerve cells.
abstract: Activin is a neurotrophic and neuroprotective factor in the central nervous system. Activin receptor-interacting protein 1 and 2 (ARIP1 and ARIP2) are identified as activin signal proteins in mouse brain. However, whether ARIP1 and ARIP2 are co-expressed in nerve cells and the differences of their biological activities are not well characterized. In the present study, we found that ARIP1 and ARIP2 mRNA expressions were detectable in mouse brain and their proteins were co-localized at the hypothalamus of cerebrum and granular layers in cerebellum, especially in Purkinje cells. Furthermore, ARIP1 and ARIP2 were co-expressed in mouse Neuro-2a cells, which is similar to the co-localization of ARIP1 and ARIP2 in hypothalamus neurons and Purkinje cells. Overexpression of ARIP1 in Neuro-2a cells inhibited activin signal transduction induced by activin A and Smad3, and activin A-induced voltage-gated Na(+) current (INa), while ARIP2 was only a negative regulator of signal transduction induced by activin A and did not alter activin A-induced INa. Taken together, these data demonstrate that ARIP1 and ARIP2 are co-expressed in some nerve cells and their biological activities are distinct.
authors.affiliation: Department of Physiology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Ge
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Liu
authors.forename: Yun-Man
authors.initials: YM
authors.lastname: Li
authors.forename: Zhong-Hui
authors.initials: ZH
authors.lastname: Liu
authors.forename: Xue-Ling
authors.initials: XL
authors.lastname: Cui
mesh_terms: D028341:Activins / Q000502:physiology; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D045744:Cell Line, Tumor; D051528:Guanylate Kinases / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism; D015398:Signal Transduction; D061566:Voltage-Gated Sodium Channels / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; C457224:Synj2bp protein, mouse; D061566:Voltage-Gated Sodium Channels; D028341:Activins; D051528:Guanylate Kinases; C405684:Magi2 protein, mouse