title: In Vitro and In Vivo Evaluation of Polyherbal Formulation against Russell's Viper and Cobra Venom and Screening of Bioactive Components by Docking Studies.
abstract: The present study emphasizes to reveal the antivenom activity of Aristolochia bracteolata Lam., Tylophora indica (Burm.f.) Merrill, and Leucas aspera S. which were evaluated against venoms of Daboia russelli russelli (Russell's viper) and Naja naja (Indian cobra). The aqueous extracts of leaves and roots of the above-mentioned plants and their polyherbal (1 : 1 : 1) formulation at a dose of 200 mg/kg showed protection against envenomed mice with LD50 doses of 0.44 mg/kg and 0.28 mg/kg against Russell's viper and cobra venom, respectively. In in vitro antioxidant activities sample extracts showed free radical scavenging effects in dose dependent manner. Computational drug design and docking studies were carried out to predict the neutralizing principles of type I phospholipase A2 (PLA2) from Indian common krait venom. This confirmed that aristolochic acid and leucasin can neutralize type I PLA2 enzyme. Results suggest that these plants could serve as a source of natural antioxidants and common antidote for snake bite. However, further studies are needed to identify the lead molecule responsible for antidote activity.
authors.affiliation: Department of Nanotechnology, Noorul Islam Centre for Higher Education, Kumaracoil, Thuckalay, Tamilnadu 629180, India ; Pharmacology Laboratory, Medicinal Plants & Horticultural Resources Division, Institute of Bioresources & Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal, Manipur 795001, India.
authors.forename: G
authors.initials: G
authors.lastname: Sakthivel
authors.forename: Amitabha
authors.initials: A
authors.lastname: Dey
authors.forename: Kh
authors.initials: Kh
authors.lastname: Nongalleima
authors.forename: Murthy
authors.initials: M
authors.lastname: Chavali
authors.forename: R S
authors.initials: RS
authors.lastname: Rimal Isaac
authors.forename: N Surjit
authors.initials: NS
authors.lastname: Singh
authors.forename: Lokesh
authors.initials: L
authors.lastname: Deb