title: Combination of fibrin-agarose hydrogels and adipose-derived mesenchymal stem cells for peripheral nerve regeneration.
abstract: OBJECTIVE
  The objective was to study the effectiveness of a commercially available collagen conduit filled with fibrin-agarose hydrogels alone or with fibrin-agarose hydrogels containing autologous adipose-derived mesenchymal stem cells (ADMSCs) in a rat sciatic nerve injury model.
  
  
  METHODS
  A 10 mm gap was created in the sciatic nerve of 48 rats and repaired using saline-filled collagen conduits or collagen conduits filled with fibrin-agarose hydrogels alone (acellular conduits) or with hydrogels containing ADMSCs (ADMSC conduits). Nerve regeneration was assessed in clinical, electrophysiological and histological studies.
  
  
  RESULTS
  Clinical and electrophysiological outcomes were more favorable with ADMSC conduits than with the acellular or saline conduits, evidencing a significant recovery of sensory and motor functions. Histological analysis showed that ADMSC conduits produce more effective nerve regeneration by Schwann cells, with higher remyelination and properly oriented axonal growth that reached the distal areas of the grafted conduits, and with intensely positive expressions of S100, neurofilament and laminin. Extracellular matrix was also more abundant and better organized around regenerated nerve tissues with ADMSC conduits than those with acellular or saline conduits.
  
  
  CONCLUSIONS
  Clinical, electrophysiological and histological improvements obtained with tissue-engineered ADMSC conduits may contribute to enhancing axonal regeneration by Schwann cells.
authors.affiliation: Department of Histology (Tissue Engineering Group), University of Granada, Avenida de Madrid 11, E-18012 Granada, Spain. vcarriel@ugr.es
authors.forename: Víctor
authors.initials: V
authors.lastname: Carriel
authors.forename: Juan
authors.initials: J
authors.lastname: Garrido-Gómez
authors.forename: Pedro
authors.initials: P
authors.lastname: Hernández-Cortés
authors.forename: Ingrid
authors.initials: I
authors.lastname: Garzón
authors.forename: Salomé
authors.initials: S
authors.lastname: García-García
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Sáez-Moreno
authors.forename: María
authors.initials: M
authors.lastname: Del Carmen Sánchez-Quevedo
authors.forename: Antonio
authors.initials: A
authors.lastname: Campos
authors.forename: Miguel
authors.initials: M
authors.lastname: Alaminos
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000671:Amputation, Surgical; D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D004576:Electromyography; D055724:Electrophysiological Phenomena; D005337:Fibrin; D020100:Hydrogels; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D016900:Neurofilament Proteins / Q000378:metabolism; D010525:Peripheral Nerves / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries; D012685:Sepharose; D014456:Ulcer / Q000473:pathology
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D007797:Laminin; D016900:Neurofilament Proteins; D009418:S100 Proteins; D005337:Fibrin; D012685:Sepharose