title: Homeostatic epithelial renewal in the gut is required for dampening a fatal systemic wound response in Drosophila.
abstract: Effective defense responses involve the entire organism. To maintain body homeostasis after tissue damage, a systemic wound response is induced in which the response of each tissue is tightly orchestrated to avoid incomplete recovery or an excessive, damaging response. Here, we provide evidence that in the systemic response to wounding, an apoptotic caspase pathway is activated downstream of reactive oxygen species in the midgut enterocytes (ECs), cells distant from the wound site, in Drosophila. We show that a caspase-pathway mutant has defects in homeostatic gut cell renewal and that inhibiting caspase activity in fly ECs results in the production of systemic lethal factors after wounding. Our results indicate that wounding remotely controls caspase activity in ECs, which activates the tissue stem cell regeneration pathway in the gut to dampen the dangerous systemic wound reaction.
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, 113-0033, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Takeishi
authors.forename: Erina
authors.initials: E
authors.lastname: Kuranaga
authors.forename: Ayako
authors.initials: A
authors.lastname: Tonoki
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Misaki
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yonemura
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanuka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Miura
mesh_terms: D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D004330:Drosophila / Q000378:metabolism* / Q000502:physiology; D020895:Enterocytes / Q000378:metabolism* / Q000502:physiology; D006706:Homeostasis; D007422:Intestines / Q000293:injuries; D009154:Mutation; D017382:Reactive Oxygen Species / Q000378:metabolism; D014945:Wound Healing
chemical_list: D017382:Reactive Oxygen Species; D020169:Caspases