title: Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation.
abstract: Fuel ethanol production is far more costly to produce than fossil fuels. There are a number of approaches to cost-effective fuel ethanol production from biomass. We characterized stress response of thermotolerant Saccharomyces cerevisiae KNU5377 during glucose-based batch fermentation at high temperature (40°C). S. cerevisiae KNU5377 (KNU5377) transcription factors (Hsf1, Msn2/4, and Yap1), metabolic enzymes (hexokinase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and alcohol dehydrogenase), antioxidant enzymes (thioredoxin 3, thioredoxin reductase, and porin), and molecular chaperones and its cofactors (Hsp104, Hsp82, Hsp60, Hsp42, Hsp30, Hsp26, Cpr1, Sti1, and Zpr1) are upregulated during fermentation, in comparison to S. cerevisiae S288C (S288C). Expression of glyceraldehyde-3-phosphate dehydrogenase increased significantly in KNU5377 cells. In addition, cellular hydroperoxide and protein oxidation, particularly lipid peroxidation of triosephosphate isomerase, was lower in KNU5377 than in S288C. Thus, KNU5377 activates various cell rescue proteins through transcription activators, improving tolerance and increasing alcohol yield by rapidly responding to fermentation stress through redox homeostasis and proteostasis.
authors.affiliation: Advanced Bio-resource Research Center, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Il-Sup
authors.initials: IS
authors.lastname: Kim
authors.forename: Young-Saeng
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
authors.forename: Ingnyol
authors.initials: I
authors.lastname: Jin
authors.forename: Ho-Sung
authors.initials: HS
authors.lastname: Yoon
mesh_terms: D000222:Adaptation, Physiological; D056804:Biofuels; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D050296:Microbial Viability; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D056804:Biofuels; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D005947:Glucose