title: An integrative analysis of four CESA isoforms specific for fiber cellulose production between Gossypium hirsutum and Gossypium barbadense.
abstract: Cotton fiber is an excellent model system of cellulose biosynthesis; however, it has not been widely studied due to the lack of information about the cellulose synthase (CESA) family of genes in cotton. In this study, we initially identified six full-length CESA genes designated as GhCESA5-GhCESA10. Phylogenetic analysis and gene co-expression profiling revealed that CESA1, CESA2, CESA7, and CESA8 were the major isoforms for secondary cell wall biosynthesis, whereas CESA3, CESA5, CESA6, CESA9, and CESA10 should involve in primary cell wall formation for cotton fiber initiation and elongation. Using integrative analysis of gene expression patterns, CESA protein levels, and cellulose biosynthesis in vivo, we detected that CESA8 could play an enhancing role for rapid and massive cellulose accumulation in Gossypium hirsutum and Gossypium barbadense. We found that CESA2 displayed a major expression in non-fiber tissues and that CESA1, a housekeeping gene like, was predominantly expressed in all tissues. Further, a dynamic alteration was observed in cell wall composition and a significant discrepancy was observed between the cotton species during fiber elongation, suggesting that pectin accumulation and xyloglucan reduction might contribute to cell wall transition. In addition, we discussed that callose synthesis might be regulated in vivo for massive cellulose production during active secondary cell wall biosynthesis in cotton fibers.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ao
authors.initials: A
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Xia
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.forename: Tingting
authors.initials: T
authors.lastname: Chen
authors.forename: Xianliang
authors.initials: X
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Fan
authors.forename: Ruyi
authors.initials: R
authors.lastname: Wang
authors.forename: Shengqiu
authors.initials: S
authors.lastname: Feng
authors.forename: Yanting
authors.initials: Y
authors.lastname: Wang
authors.forename: Bingrui
authors.initials: B
authors.lastname: Wang
authors.forename: Liangcai
authors.initials: L
authors.lastname: Peng
mesh_terms: D015153:Blotting, Western; D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis*; D032561:Cotton Fiber; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics; D018546:Hypocotyl / Q000201:enzymology / Q000254:growth & development; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase