title: Multi-labeled functionalized C₆₀ nanohybrid as tracing tag for ultrasensitive electrochemical aptasensing.
abstract: This work reports a new supramolecular method for the synthesis of the amino and thiol groups functionalized C₆₀ nanoparticles (FC60NPs) with the large surface active sites and good water solubility. First, Prussian blue carried gold nanoparticles were decorated onto the surface of the obtained FC₆₀NPs (abbreviated as Au@PB/FC₆₀). Subsequently, the Au@PB/FC₆₀ was labeled by detection aptamers and alkaline phosphatase to act as tracer. On the other hand, onion-like mesoporous graphene sheets and gold nanoparticles were utilized as the biosensor platform to immobilize a large amount of capture aptamers, owing to theirs porous structure and high surface-to-volume ratio. Based on the sandwich format, a dual signal amplification strategy based on multi-labeled functionalized C₆₀ nanohybrid as tracing tag has been successfully developed for platelet-derived growth factor B-chain electrochemical detection with a wide linear response in the range of 0.002-40 nM and a limit of detection of 0.6 pM (S/N=3). The proposed aptasensor demonstrated good specificity and high sensitivity, implying potential applications in bioanalysis and biomedicine.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhu
authors.forename: Ni
authors.initials: N
authors.lastname: Liao
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D037741:Fullerenes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D037741:Fullerenes; D020574:Proto-Oncogene Proteins c-sis; D006046:Gold; C069837:fullerene C60