title: Prevention of diet-induced obesity by apple polyphenols in Wistar rats through regulation of adipocyte gene expression and DNA methylation patterns.
abstract: This study was conducted to determine the mechanisms implicated in the beneficial effects of apple polyphenols (APs) against diet-induced obesity in Wistar rats, described in a previous study from our group. Supplementation of high-fat sucrose diet with AP prevented adiposity increase by inhibition of adipocyte hypertrophy. Rats supplemented with AP exhibited improved glucose tolerance while adipocytes isolated from these rats showed an enhanced lipolytic response to isoproterenol. AP intake led to reduced Lep, Plin, and sterol regulatory element binding transcription factor 1 (Srebf1) mRNA levels and increased aquaporin 7 (Aqp7), adipocyte enhancer binding protein 1 (Aebp1), and peroxisome proliferator-activated receptor gamma co-activator 1 alpha (Ppargc1a) mRNA levels in epididymal adipocytes. In addition, we found different methylation patterns of Aqp7, Lep, Ppargc1a, and Srebf1 promoters in adipocytes from apple-supplemented rats compared to high-fat sucrose fed rats. The administration of AP protects against body weight gain and fat deposition and improves glucose tolerance in rats. We propose that AP exerts the antiobesity effects through the regulation of genes involved in adipogenesis, lipolysis, and fatty acid oxidation, in a process that could be mediated in part by epigenetic mechanisms.
authors.affiliation: Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, Pamplona, Spain.
authors.forename: Noemi
authors.initials: N
authors.lastname: Boqué
authors.forename: Rocío
authors.initials: R
authors.lastname: de la Iglesia
authors.forename: Ana L
authors.initials: AL
authors.lastname: de la Garza
authors.forename: Fermín I
authors.initials: FI
authors.lastname: Milagro
authors.forename: Mónica
authors.initials: M
authors.lastname: Olivares
authors.forename: Oscar
authors.initials: O
authors.lastname: Bañuelos
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Soria
authors.forename: Sonia
authors.initials: S
authors.lastname: Rodríguez-Sánchez
authors.forename: José Alfredo
authors.initials: JA
authors.lastname: Martínez
authors.forename: Javier
authors.initials: J
authors.lastname: Campión
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology; D020346:Aquaporins / Q000235:genetics; D019175:DNA Methylation / Q000187:drug effects; D004032:Diet / Q000009:adverse effects; D059305:Diet, High-Fat / Q000009:adverse effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D027845:Malus / Q000737:chemistry*; D009765:Obesity / Q000209:etiology* / Q000517:prevention & control*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D059808:Polyphenols / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D019440:Anti-Obesity Agents; C491926:Aqp7 protein, rat; D020346:Aquaporins; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D059808:Polyphenols; C501429:Ppargc1a protein, rat; C497389:Srebf1 protein, rat; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; D007545:Isoproterenol
keywords: Adiposity; Epigenetics; High-fat sucrose diet; Lipolysis; Nutrigenomics