title: Effects of strigolactone-biosynthesis inhibitor TIS108 on Arabidopsis.
abstract: TIS108 is a triazole-type strigolactone (SL)-biosynthesis inhibitor that reduces the level of 2'-epi-5-deoxystrigol (epi-5DS) in rice. Here we report the effects of TIS108 on Arabidopsis. Treatment of TIS108 increased the number of branches and repressed root hair elongation as was observed in SL-deficient mutants, and co-application of GR24, a synthetic SL analog, recovered the TIS108-induced phenotype to that of wild-type. In addition, MAX3 and MAX4 genes in the SL-biosynthesis pathway were upregulated in TIS108-treated Arabidopsis, probably due to feedback regulation caused by SL deficiency. These results indicate that TIS108 is an effective tool for regulating SL production in Arabidopsis.
authors.affiliation: Department of Applied Biological Chemistry; The University of Tokyo; Tokyo, Japan; Department of Bioscience; Faculty of Applied Bioscience; Tokyo University of Agriculture; Tokyo, Japan.
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Ito
authors.forename: Mikihisa
authors.initials: M
authors.lastname: Umehara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hanada
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Tadao
authors.initials: T
authors.lastname: Asami
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006588:Hexanones / Q000737:chemistry / Q000494:pharmacology*; D007783:Lactones / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014230:Triazoles / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C000588889:6-phenoxy-1-phenyl-2-(1H-1,2,4-trizol-1-yl)hexan-1-one; D006588:Hexanones; D007783:Lactones; D014230:Triazoles
keywords: arabidopsis; biosynthesis inhibitor; plant growth regulator; plant hormone; strigolactone