title: Mid-infrared lifetime imaging for viability evaluation of lettuce seeds based on time-dependent thermal decay characterization.
abstract: An infrared lifetime thermal imaging technique for the measurement of lettuce seed viability was evaluated. Thermal emission signals from mid-infrared images of healthy seeds and seeds aged for 24, 48, and 72 h were obtained and reconstructed using regression analysis. The emission signals were fitted with a two-term exponential model that had two amplitudes and two time variables as lifetime parameters. The lifetime thermal decay parameters were significantly different for seeds with different aging times. Single-seed viability was visualized using thermal lifetime images constructed from the calculated lifetime parameter values. The time-dependent thermal signal decay characteristics, along with the decay amplitude and delay time images, can be used to distinguish aged lettuce seeds from normal seeds.
authors.affiliation: Center for Analytical Instrumentation Development, Korea Basic Science Institute, Yuseong-gu, Daejeon, Korea. ghiseok@kbsi.re.kr
authors.forename: Ghiseok
authors.initials: G
authors.lastname: Kim
authors.forename: Geon Hee
authors.initials: GH
authors.lastname: Kim
authors.forename: Chi-Kook
authors.initials: CK
authors.lastname: Ahn
authors.forename: Yoonkyu
authors.initials: Y
authors.lastname: Yoo
authors.forename: Byoung-Kwan
authors.initials: BK
authors.lastname: Cho
mesh_terms: D015374:Biosensing Techniques; D002470:Cell Survival; D018525:Germination; D006801:Humans; D007259:Infrared Rays; D018545:Lactuca / Q000166:cytology / Q000254:growth & development; D012044:Regression Analysis; D012639:Seeds / Q000166:cytology / Q000254:growth & development; D013696:Temperature