title: Miniature probe combining optical-resolution photoacoustic microscopy and optical coherence tomography for in vivo microcirculation study.
abstract: Photoacoustic microscopy (PAM) is sensitive to optical absorption, while optical coherence tomography (OCT) is based on optical backscattering. Combining PAM and OCT can provide complementary information about biological tissue. Here we present a combined optical-resolution PAM (ORPAM) and OCT system that is integrated through a miniature probe with an overall diameter of 2.3 mm, suitable for insertion through a standard endoscopic or laparoscopic port during minimally invasive surgery or endoscopic exam. The hybrid probe consists of a common optical path for OCT (light delivery/detection) and ORPAM (light excitation) and a 10 MHz unfocused ultrasound transducer for photoacoustic detection. The combined system yields a lateral resolution of 15 μm for both ORPAM and OCT.
authors.affiliation: Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Xi
authors.forename: Can
authors.initials: C
authors.lastname: Duan
authors.forename: Huikai
authors.initials: H
authors.lastname: Xie
authors.forename: Huabei
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D007909:Lenses; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D055806:Microvessels / Q000166:cytology*; D008904:Miniaturization; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D013382:Subtraction Technique / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*; D014159:Transducers