title: Mechanical control of epithelial lumen formation.
abstract: Epithelial cells differentiate and polarize to build complete epithelial organs during development. The study of epithelial morphogenesis is instrumental to the understanding of disease processes where epithelial polarity is disrupted. Recently, we demonstrated that matrix-induced cell confinement controls the acquisition of three-dimensional epithelial polarity, by modulating the initiation of the apical membrane to form a central lumen (J Cell Biol 2012; 198:1011-1026). Cell confinement can be achieved by use of micropatterned culture chips that allow precise micrometric-scale control of the cell adhesion surface and its composition. Using micropattern chips, we demonstrated that polarizing epithelial cells require high confinement conditions to properly position the centrosome and the trafficking machinery toward the cell-cell contacts and to initiate lumen morphogenesis. Low confinement induces LKB1 and RhoA-mediated cell contractility, which inhibits this mechanism for lumen formation. Deactivation of Myosin-II-mediated contractility rescued normal lumen initiation in low confinement conditions. Our results indicate that a mechanotransduction pathway coordinates nuclear and centrosome positioning to initiate epithelial morphogenesis. Here we discuss the potential candidates that control this process, specifically the polarized activation of Rho and Rab-family GTPases, and also a group of recently characterized nuclear transcription factors.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Alejo E
authors.initials: AE
authors.lastname: Rodríguez-Fraticelli
authors.forename: Fernando
authors.initials: F
authors.lastname: Martín-Belmonte
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D018385:Centrosome / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology*; D007797:Laminin / Q000378:metabolism*; D009024:Morphogenesis / Q000502:physiology*
chemical_list: D007797:Laminin
keywords: MDCK; Rab; Rho; ciliogenesis; epithelial cell polarity; lumen formation; mechanotransduction