title: In vitro modification of cisplatin cytotoxicity with magnetic fluid.
abstract: OBJECTIVE
  To study cytotoxicity of cisplatin conjugated with magnetic fluid (nanocomposite) upon exposure to magnetic field on sensitive and resistant to cisplatin MCF-7 human breast cancer cells.
  
  
  METHODS
  Cytotoxic activity was evaluated by MTT-test, intracellular iron accumulation was analyzed cytochemically, genotoxicity was studied by micronucleus test and DNA comet assay, ultrastructure was studied by electron microscopy techniques.
  
  
  RESULTS
  Nanocomposite of cisplatin was more toxic to MCF-7/S and MCF-7/CP cells compared to cisplatin in conventional pharmaceutical form. In nanocomposite-treated cells we observed more expressed signs of dystrophy (especially following application of magnetic field) and drastic alterations of nuclei ultrastructure with significant accumulation of iron nanoparticle clusters. The potent toxic action of nanocomposite is confirmed by electron microscopy and by marked genotoxicity, especially against MCF-7/CP cells.
  
  
  CONCLUSIONS
  The enhancement of cyto- and genotoxicity of cisplatin nanocomposite combined with magnetic field in comparison with effect of convetntional cisplatin alone was demonstrated.
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: V F
authors.initials: VF
authors.lastname: Chekhun
authors.forename: O V
authors.initials: OV
authors.lastname: Yurchenko
authors.forename: L A
authors.initials: LA
authors.lastname: Naleskina
authors.forename: D V
authors.initials: DV
authors.lastname: Demash
authors.forename: N Yu
authors.initials: NY
authors.lastname: Lukianova
authors.forename: Yu V
authors.initials: YV
authors.lastname: Lozovska
mesh_terms: D001943:Breast Neoplasms / Q000098:blood supply* / Q000188:drug therapy; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000378:metabolism / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D053761:Nanocomposites
chemical_list: D058185:Magnetite Nanoparticles; D002945:Cisplatin