title: Simultaneous detection of lactate and glucose by integrated printed circuit board based array sensing chip.
abstract: An integrated printed circuit board (PCB) based array sensing chip was developed to simultaneously detect lactate and glucose in mouse serum. The novelty of the chip relies on a concept demonstration of inexpensive high-throughput electronic biochip, a chip design for high signal to noise ratio and high sensitivity by construction of positively charged chitosan/redox polymer Polyvinylimidazole-Os (PVI-Os)/carbon nanotube (CNT) composite sensing platform, in which the positively charged chitosan/PVI-Os is mediator and electrostatically immobilizes the negatively charged enzyme, while CNTs function as an essential cross-linker to network PVI-Os and chitosan due to its negative charged nature. Additional electrodes on the chip with the same sensing layer but without enzymes were prepared to correct the interferences for high specificity. Low detection limits of 0.6 μM and 5 μM were achieved for lactate and glucose, respectively. This work could be extended to inexpensive array sensing chips with high sensitivity, good specificity and high reproducibility for various sensor applications.
authors.affiliation: Institute for Clean Energy and Advanced Materials, Southwest University, Chongqing 400715, PR China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Li
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zang
authors.forename: Yingshuai
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000191:economics / Q000295:instrumentation*; D002138:Calibration; D055615:Electrical Equipment and Supplies; D004563:Electrochemistry; D005947:Glucose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D056656:Lab-On-A-Chip Devices; D019344:Lactic Acid / Q000097:blood*; D051379:Mice; D046208:Microchip Analytical Procedures / Q000191:economics; D011327:Printing; D013696:Temperature; D013997:Time Factors
chemical_list: D019344:Lactic Acid; D005947:Glucose