title: Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses.
abstract: BACKGROUND
  The context and purpose of the study included 1) bacterial expression of viral protein 6 (VP6) of porcine rotavirus (PRV) and generation of rabbit polyclonal antiserum to the VP6 protein; 3) establishment of a discrimination ELISA to distinguish PRV from a panel of other porcine viruses.
  
  
  RESULTS
  The VP6 gene of PRV isolate DN30209 amplified by reverse transcription-PCR was 1356 bp containing a complete open reading frame (ORF) encoding 397 amino acids. Sequence comparison and phylogenetic analysis indicated that PRV DN30209 may belong to group A of rotavirus. Bacterially expressed VP6 was expressed in E.coli and anti-VP6 antibody was capable of distinguishing PRV from Porcine transmissible gastroenteritis virus, Porcine epidemic diarrhea virus, Porcine circovirus type II, Porcine reproductive and respiratory syndrome virus, Porcine pseudorabies virus and Porcine parvovirus.
  
  
  CONCLUSIONS
  PRV VP6 expressed in E. coli can be used to generate antibodies in rabbit; anti-VP6 serum antibody can be used as good diagnostic reagents for detection of PRV.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin, Xiangfang District 150030, China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Zhu
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Liyan
authors.initials: L
authors.lastname: Cao
authors.forename: Xiujing
authors.initials: X
authors.lastname: Dou
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhao
authors.forename: Weijuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Fan
authors.initials: F
authors.lastname: Ding
authors.forename: Ri-E
authors.initials: RE
authors.lastname: Bu
authors.forename: Siqingaowa
authors.initials: S
authors.lastname: Suo
authors.forename: Yudong
authors.initials: Y
authors.lastname: Ren
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012401:Rotavirus / Q000145:classification* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C061332:VP6 protein, Rotavirus