title: Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold.
abstract: We have prepared SAMs containing 8-mercaptooctyl α-D-mannopyranoside, either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding near 1:9 solution molar ratio of thiolatedα-mannoside to n-octanethiol whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated α-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers, and also to image the bound Con A protein.
authors.affiliation: Department of Chemistry and Biochemistry, University of Missouri - Saint Louis, Saint Louis, MO 63121, USA ; Center for Nanoscience, University of Missouri - Saint Louis, Saint Louis, MO 63121, USA.
authors.forename: Binod
authors.initials: B
authors.lastname: Pandey
authors.forename: Yih Horng
authors.initials: YH
authors.lastname: Tan
authors.forename: Kohki
authors.initials: K
authors.lastname: Fujikawa
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Demchenko
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Stine
keywords: 8-mercaptooctylα-D-mannopyranoside; Atomic Force Microscopy; Concanavalin A; Electrochemical Impedance Spectroscopy; Lectin; Nanoporous gold; Scanning Electron Microscopy; Self-assembled monolayer