title: Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins.
abstract: The aim of the present study was to analyze the protein composition of ductal breast carcinoma and the surrounding normal tissue in individual patients using comparative 2D proteomics and mass spectrometry to detect candidate disease biomarkers for diagnosis and prognosis. Samples of normal and cancerous tissue obtained form 28 patients were analyzed. Chaperonins and cytoskeletal proteins predominated among the 11 proteins for which major changes in abundance were detected. Of these 11 proteins with an altered expression, 2 had a decreased expression and 9 had an increased expression. In addition, the abundance of a few cytokeratins was also altered; however, they were not capable of serving as specific circulatory biomarkers. The proteins which we observed to exhibit an altered expression in infiltrating ductal breast carcinoma may be exploited as novel targets for therapeutic interventions or represent novel diagnostic/prognostic markers for the early detection of aggressive tumors, particularly those with multridrug-resistant phenotypes during the earlier stages of the disease.
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Carcoforo
authors.forename: Blendi
authors.initials: B
authors.lastname: Ura
authors.forename: Carlo
authors.initials: C
authors.lastname: Mischiati
authors.forename: Monica
authors.initials: M
authors.lastname: Squerzanti
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Lanzara
authors.forename: Carlo
authors.initials: C
authors.lastname: Cervellati
authors.forename: Roberta
authors.initials: R
authors.lastname: Calza
authors.forename: Patrizia Polverino
authors.initials: PP
authors.lastname: De Laureto
authors.forename: Erica
authors.initials: E
authors.lastname: Frare
authors.forename: Mattia
authors.initials: M
authors.lastname: Portinari
authors.forename: Giordana
authors.initials: G
authors.lastname: Feriotto
authors.forename: Serena
authors.initials: S
authors.lastname: Lanzara
authors.forename: Enzo
authors.initials: E
authors.lastname: Agostinelli
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Bergamini
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism*; D018833:Chaperonins / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D003598:Cytoskeletal Proteins; D009363:Neoplasm Proteins; D018833:Chaperonins