title: Mitochondrially-encoded protein Var1 promotes loss of respiratory function in Saccharomyces cerevisiae under stressful conditions.
abstract: Stressed Saccharomyces cerevisiae cells easily lose respiratory function due to deletions in mitochondrial DNA, and this increases their general stress resistance. Is the loss active? We found that erythromycin (an inhibitor of mitochondrial translation) prevents the loss in control cells but not in the ones expressing mitochondrially-encoded protein Var1 in the nucleus. Var1 is a component of mitochondrial ribosomes; it is hydrophilic, positively charged, and prone to aggregation. Addition of DNase altered Var1 content in a preparation of mitochondrial nucleoids. Our data indicate that Var1 physically interacts with mitochondrial DNA and under stress negatively regulates its maintenance.
authors.affiliation: Faculty of Bioengineering and Bioinformatics, Moscow State University, Vorobyevy Gory 1, Moscow, Russia.
authors.forename: Alexandra V
authors.initials: AV
authors.lastname: Litvinchuk
authors.forename: Svyatoslav S
authors.initials: SS
authors.lastname: Sokolov
authors.forename: Anton G
authors.initials: AG
authors.lastname: Rogov
authors.forename: Olga V
authors.initials: OV
authors.lastname: Markova
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Knorre
authors.forename: Fedor F
authors.initials: FF
authors.lastname: Severin
mesh_terms: D000332:Aerobiosis; D002467:Cell Nucleus / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D004917:Erythromycin / Q000494:pharmacology; D018869:Heat-Shock Response; D008565:Membrane Proteins / Q000502:physiology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000502:physiology*; D010101:Oxygen Consumption; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*
chemical_list: D004272:DNA, Mitochondrial; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011500:Protein Synthesis Inhibitors; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C026383:VAR1 protein, S cerevisiae; D004917:Erythromycin