title: [Effect of nitrotyrosine on renal expressions of NF-κB, MCP-1 and TGF-β1 in rats with diabetic nephropathy].
abstract: OBJECTIVE
  To observe the effect of nitrotyrosine on renal expressions of nuclear factor-κB (NF-κB), monocyte chemoattractant protein-1 (MCP-1), and transforming growth factor-β1 (TGF-β1) in rats with diabetic nephropathy (DN).
  
  
  METHODS
  Rat DN models established by a single intraperitoneal injection of streptozotocin (STZ) were randomly allocated into model group, nitrotyrosine group and ebselen group, with untreated rats as the normal control group. The rats were given the corresponding drugs for 8 weeks, and after the last administration, the 24-h urinary protein level was measured and the kidneys of the rats were harvested for detecting the protein and mRNA expressions of NF-κB, MCP-1 and TGF-β1 with immunohistochemistry and RT-PCR, respectively. The pathological changes of the kidneys were assessed microscopically.
  
  
  RESULTS
  Compared with those in the model group, the 24-h urinary protein level and expressions of NF-κB, MCP-1 and TGF-β1 mRNA and protein in the renal tissues were significantly increased by nitrotyrosine treatment, which also caused worsened renal pathology, while treatment with ebselen significantly ameliorated these changes.
  
  
  CONCLUSIONS
  Nitrotyrosine can up-regulate the mRNA and protein expressions of NF-κB, MCP-1 and TGF-β1 and aggravate the inflammatory reaction in the renal tissue of DN rats to promote the progression of DN.
authors.affiliation: Department of Emergency Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. xzz1008@163.com
authors.forename: Zhao-Zhong
authors.initials: ZZ
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Yan-Jing
authors.initials: YJ
authors.lastname: Wang
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Niu
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Li
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Long
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental; D003928:Diabetic Nephropathies / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D016328:NF-kappa B; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; C002744:3-nitrotyrosine; D014443:Tyrosine