title: STED microscopy of living cells--new frontiers in membrane and neurobiology.
abstract: Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current approaches to super-resolution optical microscopy and present the implementation of STED microscopy for novel insights into live cell mechanisms, with a focus on neurobiology and plasma membrane dynamics.
authors.affiliation: Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. ceggeli@gwdg.de
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Katrin I
authors.initials: KI
authors.lastname: Willig
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Barrantes
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002477:Cells / Q000166:cytology; D006801:Humans; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D009446:Neurobiology
keywords: STED nanoscopy; fluorescence imaging; membrane dynamics