title: Proteomic-based analysis for identification of proteins involved in 5-fluorouracil resistance in hepatocellular carcinoma.
abstract: BACKGROUND
  Hepatocellular carcinoma (HCC) has high mortality partly due to acquiring drug resistance during chemotherapy treatment. Therefore, it is necessary to explore the underlying mechanism of drug resistance.
  
  
  METHODS
  We used 2-DE and MALDI-TOF-MS analysis to explore the possible molecular insight into 5-FU resistance in HCC. The differentially expressed proteins were validated by Western blot analysis.
  
  
  RESULTS
  We identified 102 unique proteins including p16, maspin, PRDX6, PSMB7, MYL6, PHB, and HSP27 with alteration in SMMC- 7721/5-FU. Furthermore, down-regulation of PRDX6 and PSMB7 enhanced SMMC-7721/5-FU cells to 5-FU sensitivity.
  
  
  CONCLUSIONS
  Our study suggests that targeting drug resistant genes such as PRDX6 and PSMB7 could be a novel approach to overcome 5-FU resistance in HCC cells.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.forename: Shukui
authors.initials: S
authors.lastname: Qin
authors.forename: Xin
authors.initials: X
authors.lastname: Hou
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Qian
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Changjie
authors.initials: C
authors.lastname: Chen
authors.forename: Qingling
authors.initials: Q
authors.lastname: Yang
authors.forename: Lucio
authors.initials: L
authors.lastname: Miele
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Medical Oncology, First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China. qiongwu68@yahoo.com.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use*; D001483:Base Sequence; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D017931:DNA Primers; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000627:therapeutic use*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D000091402:Prohibitins; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000964:Antimetabolites, Antineoplastic; D017931:DNA Primers; D009363:Neoplasm Proteins; C000717328:PHB protein, human; D000091402:Prohibitins; D005472:Fluorouracil