title: Chemically modified bovine beta-lactoglobulin inhibits human papillomavirus infection.
abstract: Previous studies have shown that 3-hydroxyphthalic anhydride-modified bovine beta-lactoglobulin is a promising anti-HIV microbicide candidate. Here we found that this chemically modified protein, designated JB01, exhibited highly potent antiviral activity against infection by human papillomaviruses (HPV), including HPV6, HPV16 and HPV18. Its anti-HPV activity was correlated with the percentage of modified lysine and arginine residues in JB01. This modified milk protein had no cytotoxicity at the concentration of 1 mg/ml, and it is highly stable at room temperature and 37 °C for at least 12 weeks. These results suggest that JB01 has good potential to be developed as an effective, safe and inexpensive antiviral agent for treatment and prevention of HPV infection.
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College and Institute of Medical Microbiology, Fudan University, Shanghai 200032, China. lul@fudan.edu.cn
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Shibo
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D052162:Human papillomavirus 16 / Q000187:drug effects* / Q000254:growth & development; D052161:Human papillomavirus 18 / Q000187:drug effects* / Q000254:growth & development; D052163:Human papillomavirus 6 / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D020128:Inhibitory Concentration 50; D007782:Lactoglobulins / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D010796:Phthalic Anhydrides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D055550:Protein Stability; D013329:Structure-Activity Relationship; D013696:Temperature; D013997:Time Factors
chemical_list: D000998:Antiviral Agents; D007782:Lactoglobulins; D010796:Phthalic Anhydrides; C478070:3-hydroxyphthalic anhydride