title: miR-16 inhibits cell proliferation by targeting IGF1R and the Raf1-MEK1/2-ERK1/2 pathway in osteosarcoma.
abstract: Several miRNAs have been implicated in the development and progression of osteosarcoma (OS). In this study, we found that miR-16 is downregulated in OS cell lines and tissues. Overexpression of miR-16 suppresses OS cell proliferation and tumor growth in nude mice. Furthermore, we confirmed that IGF1R is a direct target of miR-16. Mechanistic investigation revealed that miR-16 overexpression inhibits the Raf1-MEK1/2-ERK1/2 pathway. In clinical specimens, IGF1R levels inversely correlate with miR-16 expression. Our results provide significant clues regarding the role of miR-16 as a tumor suppressor by targeting IGF1R in OS.
authors.affiliation: Department of Orthopaedics Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Wang
authors.forename: Hua-song
authors.initials: HS
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Xi-ming
authors.initials: XM
authors.lastname: Liu
authors.forename: Xian-hua
authors.initials: XH
authors.lastname: Cai
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D006801:Humans; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D048370:MAP Kinase Kinase 2 / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*; D019908:Proto-Oncogene Proteins c-raf / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics*
chemical_list: C505362:MIRN16 microRNA, human; D035683:MicroRNAs; C482128:MAP2K2 protein, human; D017526:Receptor, IGF Type 1; D019908:Proto-Oncogene Proteins c-raf; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048369:MAP Kinase Kinase 1; D048370:MAP Kinase Kinase 2