title: Method for the preparation of mucosal flaps from the jejunum of laying hens for transporter studies in Ussing chambers.
abstract: Ussing chambers are frequently used for in vitro evaluation of intestinal transport physiology. The current study describes investigating the jejunal tissue from laying hens using a specific preparation method and evaluates the effect of glutamine in the maintenance buffer. Tunica mucosa was stripped from 104 jejunal samples from 10 hens and stabilised by a net device. Fifty samples were maintained with modified Krebs-Henseleit buffer (Control), 54 samples with additional 5 mM glutamine (Group Gln). The percentage of responding samples varied between 87 and 100%. Mean short circuit current (ΔI sc,) [µA/cm(2)] of samples exposed to 10 mM glucose in the Control group and Group Gln was 17.0 and 14.6 (p = 0.836), respectively, of samples exposed to 100 µM phloridzin -13.3 and -11.8 (p = 0.712), respectively, and of samples exposed to 100 µM carbachol 4.7 and 3.7 (p = 0.450), respectively. In conclusion, the net-supported method enabled a reliable investigation of jejunum from laying hens. Glutamine in the maintenance buffer was of no significant benefit.
authors.affiliation: Department of Veterinary Medicine , Freie Universität Berlin, Berlin, Germany. isabelle.ruhnke@fu-berlin.de
authors.forename: Isabelle
authors.initials: I
authors.lastname: Ruhnke
authors.forename: Ilen
authors.initials: I
authors.lastname: Röhe
authors.forename: Wilfried
authors.initials: W
authors.lastname: Meyer
authors.forename: Susan
authors.initials: S
authors.lastname: Kröger
authors.forename: Konrad
authors.initials: K
authors.lastname: Neumann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Zentek
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D005260:Female; D007413:Intestinal Mucosa / Q000502:physiology*; D007583:Jejunum / Q000502:physiology*; D010058:Oviposition; D013048:Specimen Handling