title: SRV: an open-source toolbox to accelerate the recovery of metabolic biomarkers and correlations from metabolic phenotyping datasets.
abstract: BACKGROUND
  Supervised multivariate statistical analyses are often required to analyze the high-density spectral information in metabolic datasets acquired from complex mixtures in metabolic phenotyping studies. Here we present an implementation of the SRV-Statistical Recoupling of Variables-algorithm as an open-source Matlab and GNU Octave toolbox. SRV allows the identification of similarity between consecutive variables resulting from the high-resolution bucketing. Similar variables are gathered to restore the spectral dependency within the datasets and identify metabolic NMR signals. The correlation and significance of these new NMR variables for a given effect under study can then be measured and represented on a loading plot to allow a visual and efficient identification of candidate biomarkers. Further on, correlations between these candidate biomarkers can be visualized on a two-dimensional pseudospectrum, representing a correlation map, helping to understand the modifications of the underlying metabolic network.
  
  
  BACKGROUND
  SRV toolbox is encoded in MATLAB R2008A (Mathworks, Natick, MA) and in GNU Octave. It is available free of charge at http://www.prabi.fr/redmine/projects/srv/repository with a tutorial.
  
  
  BACKGROUND
  benjamin.blaise@chu-lyon.fr or vincent.navratil@univ-lyon1.fr.
authors.affiliation: Centre de RMN à Très Hauts Champs, Institut des sciences analytiques, CNRS/ENS Lyon/UCB Lyon 1, Université de Lyon, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Navratil
authors.forename: Clément
authors.initials: C
authors.lastname: Pontoizeau
authors.forename: Elise
authors.initials: E
authors.lastname: Billoir
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Blaise
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000737:chemistry*; D006801:Humans; D053858:Metabolic Networks and Pathways; D015999:Multivariate Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular; D012984:Software
chemical_list: D015415:Biomarkers