title: Maturation of postnatally generated olfactory bulb granule cells depends on functional γ-protocadherin expression.
abstract: γ-protocadherins (γ-pcdhs) are transmembrane receptor proteins ubiquitously expressed in the postnatal and adult mouse brain. γ-pcdhs are required for normal neuronal development as shown for spinal cord interneurons, retinal ganglion cells and cortical neurons. To test the role of γ-pcdhs during development of subventricular zone progenitor cells and their subsequent differentiation into olfactory granule cells we generated a conditional γ-pcdh(lox/lox) allele (γ-pcdh(lox/lox)) allowing for functional γ-pcdh inactivation upon lentivirus-mediated Cre-recombinase expression selectively in subventricular zone progenitor cells. While γ-pcdh loss did not alter the proliferation of subventricular zone progenitors, γ-pcdh ko progenitors that reached the main olfactory bulb showed a significant reduction in dendritic arborization and failed to develop dendritic spines. Our results suggest that olfactory bulb granule cell maturation necessitates functional γ-pcdh expression.
authors.affiliation: Max Planck Institute for Medical Research, Jahnstr. 29, 69120 Heidelberg, Germany. julia.ledderose@mpimf-heidelberg.mpg.de
authors.forename: Julia
authors.initials: J
authors.lastname: Ledderose
authors.forename: Sandra
authors.initials: S
authors.lastname: Dieter
authors.forename: Martin K
authors.initials: MK
authors.lastname: Schwarz
mesh_terms: D000818:Animals; D000070585:Cadherin Related Proteins; D015820:Cadherins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D002455:Cell Division; D019070:Cell Lineage; D002465:Cell Movement; D048430:Cell Shape; D003712:Dendrites / Q000648:ultrastructure; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007276:Injections, Intraventricular; D019426:Integrases; D016086:Lentivirus / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D009830:Olfactory Bulb / Q000166:cytology* / Q000254:growth & development; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000502:physiology; D011995:Recombination, Genetic
chemical_list: D000070585:Cadherin Related Proteins; D015820:Cadherins; C464785:Gamma-protocadherins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases