title: Mimicking nanofibrous hybrid bone substitute for mesenchymal stem cells differentiation into osteogenesis.
abstract: Mimicking hybrid extracellular matrix is one of the main challenges for bone tissue engineering (BTE). Biocompatible polycaprolactone/poly(α,β)-DL-aspartic acid/collagen nanofibrous scaffolds were fabricated by electrospinning and nanohydroxyapatite (n-HA) was deposited by calcium phosphate dipping method for BTE. Human mesenchymal stem cells (hMSCs) were cultured on these hybrid scaffolds to investigate the cell proliferation, osteogenic differentiation by alkaline phosphatase activity, mineralization, double immunofluorescent staining using CD90 and expression of osteocalcin. The present study indicated that the PCL/PAA/collagen/n-HA scaffolds promoted greater osteogenic differentiation of hMSCs, proving to be a potential hybrid scaffolds for BTE.
authors.affiliation: Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Gandhimathi
authors.forename: Jayarama
authors.initials: J
authors.lastname: Venugopal
authors.forename: Rajeswari
authors.initials: R
authors.lastname: Ravichandran
authors.forename: Subramanian
authors.initials: S
authors.lastname: Sundarrajan
authors.forename: Shanmugavel
authors.initials: S
authors.lastname: Suganya
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D018786:Bone Substitutes / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology; D011091:Polyesters / Q000494:pharmacology; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry; D014867:Water
chemical_list: D018786:Bone Substitutes; D010455:Peptides; D011091:Polyesters; C509317:nano-hydroxyapatite-collagen; D014867:Water; D015675:Osteocalcin; C016240:polycaprolactone; C017645:polyaspartate; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase