title: High-resolution typing by MLVF unveils extensive heterogeneity of European livestock-associated methicillin-resistant Staphylococcus aureus isolates with the sequence type 398.
abstract: Methicillin-resistant Staphylococcus aureus sequence type 398 (MRSA ST398) has emerged in livestock worldwide. In particular, areas in Europe with high densities of livestock farming are affected. Consequently, the incidence of human colonization and infection with ST398 is rapidly increasing. Distinguishing different ST398 isolates with standard typing tools is problematic. The objective of this study was to examine the discriminatory power of Multiple-Locus Variable number tandem repeat Fingerprinting (MLVF) on a highly diverse ST398 collection. Our data show that MLVF combined with spa-typing is an attractive approach for high-resolution typing of ST398 isolates and unveiling their relatedness.
authors.affiliation: Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Corinna
authors.initials: C
authors.lastname: Glasner
authors.forename: Artur J
authors.initials: AJ
authors.lastname: Sabat
authors.forename: Monika A
authors.initials: MA
authors.lastname: Chlebowicz
authors.forename: Wannes
authors.initials: W
authors.lastname: Vanderhaeghen
authors.forename: Alexandra
authors.initials: A
authors.lastname: Fetsch
authors.forename: Beatriz
authors.initials: B
authors.lastname: Guerra
authors.forename: Helen
authors.initials: H
authors.lastname: Huber
authors.forename: Roger
authors.initials: R
authors.lastname: Stephan
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
authors.forename: Patrick
authors.initials: P
authors.lastname: Butaye
authors.forename: Andreas
authors.initials: A
authors.lastname: Voss
authors.forename: Mireille
authors.initials: M
authors.lastname: Wulf
authors.forename: Jan Maarten
authors.initials: JM
authors.lastname: van Dijl
mesh_terms: D000818:Animals; D002353:Carrier State / Q000453:epidemiology / Q000662:veterinary*; D016172:DNA Fingerprinting / Q000379:methods; D005060:Europe / Q000453:epidemiology; D005838:Genotype; D058751:Livestock / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods*; D058889:Molecular Typing / Q000379:methods*; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000662:veterinary*; D013205:Staphylococcal Protein A / Q000235:genetics
chemical_list: D013205:Staphylococcal Protein A