title: Anti-inflammatory cycloartane-type saponins of Astragalus membranaceus.
abstract: A new cycloartane-type triterpene glycoside, agroastragaloside V (1) was isolated from the roots of Astragalus membranaceus. The structure was identified as 3-O-β-(2'-O-acetyl)-D-xylopyranosyl-6-O-β-D-glucopyranosyl-(24S)-3β,6α,24α,25-tetrahydroxy- 9,19-cyclolanostane, by means of spectroscopic methods, including HR-FAB/MS, 1D NMR (1H, 13C, DEPT), 2D NMR (gCOSY, gHSQC, gHMBC, NOESY), and IR spectroscopy. Four known cycloartane glycosides, namely, agroastragaloside I (2), agroastragaloside II (3), isoastragaloside II (4) and astragaloside IV (5) were also isolated. All isolated compounds were tested for the ability to inhibit LPS-induced nitric oxide production in RAW264.7 macrophages.
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea.
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Noh
authors.forename: Jehun
authors.initials: J
authors.lastname: Choi
authors.forename: Kyeong-Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Yoonpyo
authors.initials: Y
authors.lastname: Hong
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Seung-Yu
authors.initials: SY
authors.lastname: Kim
authors.forename: Geum-Soog
authors.initials: GS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000737:chemistry; D027885:Astragalus propinquus / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D006027:Glycosides; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C085352:cycloartane