title: In vitro assessment of Ag2O nanoparticles toxicity against Gram-positive and Gram-negative bacteria.
abstract: In view of antibiotic resistance among pathogens, the present study is to address the toxicity of Ag2O nanoparticles against the Gram-positive and Gram-negative bacteria through in vitro assays. The preliminary screening by agar diffusion assay confirms the antibacterial activity of Ag2O nanoparticles against all the test bacteria. Comparative antibacterial activity of Ag2O nanoparticles and respective antibiotics reveals their broad range of activity and lower inhibitory dose against the used bacterial strains. Further, they can inhibit E. coli with an effective dose of 0.036 mg/ml within 1 h of exposure time as determined by luciferin based ATP assay. Moreover, the Ag2O nanoparticles exhibit higher antibacterial efficacy against Gram-negative bacteria than Gram-positive bacteria, as revealed by their MIC & MBC values. Therefore, Ag2O nanoparticles pave the way for a new generation of antibacterial agents against the emerging multidrug resistant pathogens.
authors.affiliation: Department of Microbiology, C.B.S.H., G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India.
authors.forename: Harshita
authors.initials: H
authors.lastname: Negi
authors.forename: Palaniyandi
authors.initials: P
authors.lastname: Rathinavelu Saravanan
authors.forename: Tithi
authors.initials: T
authors.lastname: Agarwal
authors.forename: Mohd
authors.initials: M
authors.lastname: Ghulam Haider Zaidi
authors.forename: Reeta
authors.initials: R
authors.lastname: Goel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D053758:Nanoparticles; D010087:Oxides / Q000494:pharmacology*; D018030:Silver Compounds / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010087:Oxides; D018030:Silver Compounds; C040225:disilver oxide