title: Modulation of hyperthermia-induced platelet aggregation inhibition in the presence of urea.
abstract: OBJECTIVE
  This study has been conducted to evaluate the effect of urea on aggregation responses of heat-treated platelets.
  
  
  METHODS
  The urea was added to platelet-rich plasma (PRP) samples in final concentrations of 50 and 100 mM. PRP samples, with or without exogenous urea, were incubated at 37 °C, 39 °C and 41 °C for 90 min and then were stimulated with adenosine diphosphate (ADP) or epinephrine for measuring of platelet aggregation responses. The average reduction in aggregability of heat-treated samples with reference to mean value obtained for control samples treated at 37 °C was expressed as inhibition percentage.
  
  
  RESULTS
  Aggregation responses of the samples treated in the presence of 50 mM and 100 mM urea were significantly less inhibited by hyperthermia treatments compared with those treated without exogenous urea.
  
  
  CONCLUSIONS
  The results indicate that the inhibitory effect of hyperthermia on platelet aggregation responses could be significantly modulated by urea.
authors.affiliation: Platelet Research Laboratory, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Aliasgharzadeh
authors.forename: Ahmad
authors.initials: A
authors.lastname: Gharehbaghian
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Taherian
authors.forename: Mehran
authors.initials: M
authors.lastname: Ghasemzadeh
authors.forename: Morteza
authors.initials: M
authors.lastname: Salimian
mesh_terms: D000244:Adenosine Diphosphate; D004837:Epinephrine; D005334:Fever; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects*; D053657:Platelet-Rich Plasma / Q000187:drug effects / Q000502:physiology; D014508:Urea / Q000008:administration & dosage*
chemical_list: D000244:Adenosine Diphosphate; D014508:Urea; D004837:Epinephrine