title: A comparison of six major platelet functional tests to assess the impact of carbon nanomaterials on platelet function: a practical guide.
abstract: The study of the haemocompatibility of nanomaterials that could be in contact with blood (e.g. nanoparticle (NP)-based drug-delivery system) is of major importance. The primary objective of this study was to compare the ability of six platelet functional tests to assess the impact of NPs on platelet function. The secondary objective was to determine an accurate and reliable screening test to measure the potential impact of NPs on primary haemostasis whatever their physicochemical properties. Four types of carbon NPs (carbon black, fullerenes, single-walled carbon nanotubes and multi-walled carbon nanotubes) were investigated on six platelet function tests: light transmission aggregometry, whole-blood impedance aggregometry, platelet function analyser-100 (PFA-100®) and Cone-and-Plate(let) analyser (Impact-R®), transmission- and field emission gun scanning electron microscopy (FEG-SEM). We considered that Impact-R® supported by FEG-SEM is the reference method to investigate the potential impact of NPs on platelet function.
authors.affiliation: Department of Pharmacy, Namur Nanosafety Center (NNC), NAmur MEdicine & Drug Innovation Center (NAMEDIC), Namur Thrombosis and Hemostasis Center (NTHC), University of Namur (FUNDP) , Namur , Belgium.
authors.forename: Julie
authors.initials: J
authors.lastname: Laloy
authors.forename: François
authors.initials: F
authors.lastname: Mullier
authors.forename: Lutfiye
authors.initials: L
authors.lastname: Alpan
authors.forename: Jorge
authors.initials: J
authors.lastname: Mejia
authors.forename: Stéphane
authors.initials: S
authors.lastname: Lucas
authors.forename: Bernard
authors.initials: B
authors.lastname: Chatelain
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
authors.forename: Bernard
authors.initials: B
authors.lastname: Masereel
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Rolin
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Dogné
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002244:Carbon / Q000633:toxicity*; D006801:Humans; D049329:Nanostructures / Q000633:toxicity*; D010979:Platelet Function Tests / Q000379:methods*; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D002244:Carbon