title: Insulin-like growth factor-I enhances proliferation and differentiation of human mesenchymal stromal cells in vitro.
abstract: Human mesenchymal stromal cells (hMSCs) offer great potential for bone tissue engineering applications, but their in vivo performance remains limited. Preconditioning of these cells with small molecules to improve their differentiation before implantation, or incorporation of growth factors are possible solutions. Insulin-like growth factor-1 (IGF-1) is one of the most abundant growth factors in bone, involved in growth, development, and metabolism, but its effects on hMSCs are still subject of debate. Here we examined the effects of IGF-1 on proliferation and differentiation of hMSCs in vitro and we found that serum abolished the effects of IGF-1. Only in the absence of serum, IGF-1 increased proliferation, alkaline phosphatase expression, and osteogenic gene expression of hMSCs. Furthermore, we examined synergistic effects of bone morphogenetic protein-2 (BMP-2) and IGF-1 and, although IGF-1 enhanced BMP-2-induced mineralization, IGF-1 only slightly affected in vivo bone formation.
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Joyce
authors.initials: J
authors.lastname: Doorn
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Roberts
authors.forename: Janneke
authors.initials: J
authors.lastname: Hilderink
authors.forename: Nathalie
authors.initials: N
authors.lastname: Groen
authors.forename: Aart
authors.initials: A
authors.lastname: van Apeldoorn
authors.forename: Clemens
authors.initials: C
authors.lastname: van Blitterswijk
authors.forename: Jan
authors.initials: J
authors.lastname: Schrooten
authors.forename: Jan
authors.initials: J
authors.lastname: de Boer
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D064347:Microscopy, Electrochemical, Scanning
chemical_list: D007334:Insulin-Like Growth Factor I