title: Application of magnetic and core-shell nanoparticles to determine enrofloxacin and its metabolite using laser induced fluorescence microscope.
abstract: A unique analytical method using nanoparticles and laser-induced fluorescence microscopy (LIFM) was developed to determine enrofloxacin in this work. For sample pretreatment, two different kinds of particles, i.e., synthesized dye-doped core-shell silica nanoparticles and magnetic micro-particles (MPs), were used for fluorescent tagging and concentrating the enrofloxacin, respectively. The antibody of enrofloxacin was immobilized on the synthesized FITC-doped core-shell nanoparticles, and the enrofloxacin target was extracted by the MPs. At this moment, the average number of antibodies on each core-shell silica nanoparticle was ~0.9, which was determined by the fluorescence ratiometric method. The described method was demonstrated for a meat sample to determine enrofloxacin using LIFM, and the result was compared with enzyme-linked immunosorbent assay (ELISA). The developed technique allowed the simplified analytical procedure, improved the detection limit about 54-fold compared to ELISA.
authors.affiliation: Department of Chemistry, NSBI, Dankook University, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Suji
authors.initials: S
authors.lastname: Kim
authors.forename: Junga
authors.initials: J
authors.lastname: Ko
authors.forename: H B
authors.initials: HB
authors.lastname: Lim
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D055910:Antibodies, Immobilized / Q000737:chemistry; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007075:Immunoglobulin M / Q000737:chemistry / Q000276:immunology; D007834:Lasers; D059346:Magnets / Q000737:chemistry*; D008460:Meat / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D055910:Antibodies, Immobilized; D024841:Fluoroquinolones; D007075:Immunoglobulin M; D000077422:Enrofloxacin