title: Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis.
abstract: Glycated proteins are important biomarkers for age-related disorders, however their analysis is challenging because of the complexity of the protein-carbohydrate adducts. Here we report a method that enables the detection and identification of individual glycated proteins in complex samples using fluorescent boronic acids in gel electrophoresis. Using this method we identified glycated proteins in human serum, insect hemolymph and mouse brain homogenates, confirming this technique as a powerful proteomics tool that can be used for the identification of potential disease biomarkers.
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK.
authors.forename: Marta P
authors.initials: MP
authors.lastname: Pereira Morais
authors.forename: Dominic
authors.initials: D
authors.lastname: Marshall
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Flower
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Caunt
authors.forename: Tony D
authors.initials: TD
authors.lastname: James
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Williams
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Waterfield
authors.forename: Jean M H
authors.initials: JM
authors.lastname: van den Elsen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001897:Boronic Acids / Q000138:chemical synthesis; D002540:Cerebral Cortex / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006031:Glycosylation; D006458:Hemolymph / Q000737:chemistry; D006801:Humans; D018402:Manduca; D051379:Mice; D040901:Proteomics / Q000379:methods; D012709:Serum Albumin / Q000032:analysis
chemical_list: D015415:Biomarkers; D001897:Boronic Acids; D005456:Fluorescent Dyes; D012709:Serum Albumin; C010686:benzeneboronic acid; D019793:Fluorescein