title: Probiotics L. plantarum and L. curvatus in combination alter hepatic lipid metabolism and suppress diet-induced obesity.
abstract: OBJECTIVE
  To determine the effects of naturally derived probiotic strains individually or combination on a short-term diet-induced obesity model.
  
  
  METHODS
  C57BL/6J mice (n = 50) were randomly divided into five groups, then fed a high-fat high-cholesterol diet (HFCD), HFCD and Lactobacillus plantarum KY1032 (PL, 10(10) cfu/day), HFCD and Lactobacillus curvatus HY7601 (CU, 10(10) cfu/day), HFCD and in combination with PL+CU (10(10) cfu/day), or a normal diet (ND) for 9 weeks.
  
  
  RESULTS
  PL and CU showed distinct and shared metabolic activity against a panel of 50 carbohydrates. Fat accumulation in adipose tissue and liver was significantly reduced by probiotic strains CU or PL+CU. Probiotic strains CU or PL+CU reduced cholesterol in plasma and liver, while PL+CL had a synergistic effect on hepatic triglycerides. Probiotic strains PL+CU combination was more effective for inhibiting gene expressions of various fatty acid synthesis enzymes in the liver, concomitant with decreases in fatty acid oxidation-related enzyme activities and their gene expressions.
  
  
  CONCLUSIONS
  Multi-strain probiotics may prove more beneficial than single-strain probiotics to combat fat accumulation and metabolic alterations in diet-induced obesity.
authors.affiliation: Department of Food Science and Nutrition, Center for Food & Nutritional Genomics, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea.
authors.forename: Sae-Rom
authors.initials: SR
authors.lastname: Yoo
authors.forename: Young-Jae
authors.initials: YJ
authors.lastname: Kim
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Park
authors.forename: Un-Ju
authors.initials: UJ
authors.lastname: Jung
authors.forename: Seon-Min
authors.initials: SM
authors.lastname: Jeon
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
authors.forename: Chul-Sung
authors.initials: CS
authors.lastname: Huh
authors.forename: Robin
authors.initials: R
authors.lastname: McGregor
authors.forename: Myung Sook
authors.initials: MS
authors.lastname: Choi
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D059305:Diet, High-Fat / Q000009:adverse effects*; D015870:Gene Expression; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007778:Lactobacillus; D050155:Lipogenesis / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000517:prevention & control*; D019936:Probiotics / Q000008:administration & dosage*; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D002791:Cholesterol, Dietary; D053583:Interleukin-1beta; D015850:Interleukin-6; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D002784:Cholesterol