title: Glucose oxidase/cellulose-carbon nanotube composite paper as a biocompatible bioelectrode for biofuel cells.
abstract: Biofuel cells are devices for generating electrical energy directly from chemical energy of renewable biomass using biocatalysts such as enzymes. Efficient electrical communication between redox enzymes and electrodes is essential for enzymatic biofuel cells. Carbon nanotubes (CNTs) have been recognized as ideal electrode materials because of their high electrical conductivity, large surface area, and inertness. Electrodes consisting entirely of CNTs, which are known as CNT paper, have high surface areas but are typically weak in mechanical strength. In this study, cellulose (CL)-CNT composite paper was fabricated as electrodes for enzymatic biofuel cells. This composite electrode was prepared by vacuum filtration of CNTs followed by reconstitution of cellulose dissolved in ionic liquid, 1-ethyl-3-methylimidazolium acetate. Glucose oxidase (GOx), which is a redox enzyme capable of oxidizing glucose as a renewable fuel using oxygen, was immobilized on the CL-CNT composite paper. Cyclic voltammograms revealed that the GOx/CL-CNT paper electrode showed a pair of well-defined peaks, which agreed well with that of FAD/FADH2, the redox center of GOx. This result clearly shows that the direct electron transfer (DET) between the GOx and the composite electrode was achieved. However, this DET was dependent on the type of CNTs. It was also found that the GOx immobilized on the composite electrode retained catalytic activity for the oxidation of glucose.
authors.affiliation: Department of Chemical and Biochemical Engineering, Dongguk University-Seoul, 30 Pildong-ro 1-gil, Jung-gu, Seoul, 100-715, Republic of Korea.
authors.forename: Keehoon
authors.initials: K
authors.lastname: Won
authors.forename: Young-Hoo
authors.initials: YH
authors.lastname: Kim
authors.forename: Seulji
authors.initials: S
authors.lastname: An
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Saerom
authors.initials: S
authors.lastname: Park
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Choi
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Kim
authors.forename: Hak-In
authors.initials: HI
authors.lastname: Hwang
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hyungsup
authors.initials: H
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lee
mesh_terms: D001674:Bioelectric Energy Sources; D002482:Cellulose / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D002482:Cellulose; D005949:Glucose Oxidase