title: GmWRKY53, a water- and salt-inducible soybean gene for rapid dissection of regulatory elements in BY-2 cell culture.
abstract: Drought is the major cause of crop losses worldwide. Water stress-inducible promoters are important for understanding the mechanisms of water stress responses in crop plants. Here we utilized tobacco (Nicotiana tabacum L.) Bright Yellow 2 (BY-2) cell system in presence of polyethylene glycol, salt and phytohormones. Extension of the system to 85 mM NaCl led to inducibility of up to 10-fold with the water stress and salt responsive soybean GmWRKY53 promoter. Upon ABA and JA treatment fold inducibility was up to 5-fold and 14-fold, respectively. Thus, we hypothesize that GmWRKY53 could be used as potential model candidate for dissecting drought regulatory elements as well as understanding crosstalk utilizing a rapid heterologous system of BY-2 culture.
authors.affiliation: Department of Biology and Microbiology; South Dakota State University; Brookings, SD USA; These authors contributed equally to this work.
authors.forename: Prateek
authors.initials: P
authors.lastname: Tripathi
authors.forename: Roel C
authors.initials: RC
authors.lastname: Rabara
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Rushton
mesh_terms: D002478:Cells, Cultured; D003681:Dehydration; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D013025:Glycine max / Q000235:genetics*; D014026:Nicotiana / Q000166:cytology*; D014867:Water / Q000494:pharmacology*
chemical_list: D010940:Plant Proteins; D014867:Water; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D005966:Glucuronidase
keywords: GmWRKY53; abscisic acid; salt stress; tobacco BY-2; water stress