title: Identification and characterization of the nano-sized vesicles released by muscle cells.
abstract: Several cell types secrete small membranous vesicles that contain cell-specific collections of proteins, lipids, and genetic material. The function of these vesicles is to allow cell-to-cell signaling and the horizontal transfer of their cargo molecules. Here, we demonstrate that muscle cells secrete nano-sized vesicles and that their release increases during muscle differentiation. Analysis of these nanovesicles allowed us to characterize them as exosome-like particles and to define the potential role of the multifunctional protein Alix in their biogenesis.
authors.affiliation: Institute of Biomedicine and Molecular Immunology A. Monroy (IBIM), National Research Council (CNR), Palermo, Italy.
authors.forename: Daniele P
authors.initials: DP
authors.lastname: Romancino
authors.forename: Gaetano
authors.initials: G
authors.lastname: Paterniti
authors.forename: Yvan
authors.initials: Y
authors.lastname: Campos
authors.forename: Angela
authors.initials: A
authors.lastname: De Luca
authors.forename: Valentina
authors.initials: V
authors.lastname: Di Felice
authors.forename: Alessandra
authors.initials: A
authors.lastname: d'Azzo
authors.forename: Antonella
authors.initials: A
authors.lastname: Bongiovanni
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism*; D051379:Mice; D032342:Muscle Cells / Q000166:cytology* / Q000378:metabolism; D049329:Nanostructures
chemical_list: D002135:Calcium-Binding Proteins; C492144:Pdcd6ip protein, mouse