title: Identification of a nonstructural DNA-binding protein (DBP) as an antigen with diagnostic potential for human adenovirus.
abstract: BACKGROUND
  Human adenoviruses (HAdVs) have been implicated as important agents in a wide range of human illnesses. To date, 58 distinct HAdV serotypes have been identified and can be grouped into six species. For the immunological diagnosis of adenoviruses, the hexon protein, a structural protein, has been used. The potential of other HAdV proteins has not been fully addressed.
  
  
  RESULTS
  In this study, a nonstructural antigenic protein, the DNA binding protein (DBP) of human adenovirus 5 and 35 (Ad5, Ad35) - was identified using immunoproteomic technology. The expression of Ad5 and Ad35 DBP in insect cells could be detected by rhesus monkey serum antibodies and healthy adult human serum positive for Ad5 and Ad35. Recombinant DBPs elicited high titer antibodies in mice. Their conserved domain displayed immunological cross-reactions with heterologous DBP antibodies in Western blot assays. DBP-IgM ELISA showed higher sensitivity adenovirus IgM detection than the commercial Adenovirus IgM Human ELISA Kit. A Western blot method developed based on Ad5 DBP was highly consistent with (χ(2) = 44.9, P<0.01) the Western blot assay for the hexon protein in the detection of IgG, but proved even more sensitive.
  
  
  CONCLUSIONS
  The HAdV nonstructural protein DBP is an antigenic protein that could serve as an alternative common antigen for adenovirus diagnosis.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Chengjun
authors.initials: C
authors.lastname: Wu
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.forename: Gláucia
authors.initials: G
authors.lastname: Paranhos-Baccalà
authors.forename: Guy
authors.initials: G
authors.lastname: Vernet
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Hung
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis*; D000260:Adenoviruses, Human / Q000145:classification / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D036022:Capsid Proteins / Q000276:immunology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C018315:hexon capsid protein, Adenovirus