title: Peptide nanofiber scaffolds for multipotent stromal cell culturing.
abstract: Self-assembled peptide nanofibers are versatile materials providing suitable platforms for regenerative medicine applications. This chapter describes the use of peptide nanofibers as extracellular matrix mimetic scaffolds for two-dimensional (2D) and three-dimensional (3D) multipotent stromal cell culture systems and procedures for in vitro experiments using these scaffolds. Preparation of 2D and 3D peptide nanofiber scaffolds and cell culturing procedures are presented as part of in vitro experiments including cell adhesion, viability, and spreading analysis. Analysis of cellular differentiation on peptide nanofiber scaffolds is described through immunocytochemistry, qRT-PCR, and other biochemical experiments towards osteogenic and chondrogenic lineage.
authors.affiliation: UNAM-Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey.
authors.forename: Seher
authors.initials: S
authors.lastname: Ustun
authors.forename: Samet
authors.initials: S
authors.lastname: Kocabey
authors.forename: Mustafa O
authors.initials: MO
authors.lastname: Guler
authors.forename: Ayse B
authors.initials: AB
authors.lastname: Tekinay
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002470:Cell Survival; D020219:Chondrogenesis; D006801:Humans; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry; D010012:Osteogenesis; D010455:Peptides / Q000737:chemistry; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D010455:Peptides