title: Mesenchymal stem cells protect against neonatal rat hyperoxic lung injury.
abstract: OBJECTIVE
  Bronchopulmonary dysplasia (BPD) is a significant global health problem and currently lacks effective therapy. We established a neonatal rat model of BPD to investigate therapeutic potential of bone marrow-derived mesenchymal stem cells (BMSCs) in neonatal hyperoxic lung injury.
  
  
  METHODS
  BMSCs were isolated, identified, and transfected by lentiviral vector carrying green fluorescent protein gene in vitro. Neonatal Sprague-Dawley rats were injected intravenously with either BMSCs or phosphate-buffered saline following 95% oxygen exposure, and assessed for the survival rate and alveolar injury during recovery.
  
  
  RESULTS
  Treatment with BMSCs after oxygen exposure for 7 days improved survival of neonatal rat during recovery. BMSCs protected against neonatal rat hyperoxic lung injury during recovery as demonstrated by enhanced expression of AQP5 and SP-C, likely due to the suppression of alveolar cell apoptosis and lung inflammation responses to oxygen with up-regulation of the expression of BCL-2 gene and down-regulation of the expression of BAX gene and stimulation of vascular endothelial growth factor and so on.
  
  
  CONCLUSIONS
  BMSCs protect against O2-mediated injury partially through stimulation of potent mediators that participate in tissue repair.
authors.affiliation: Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Department of Pediatric , Yanjiang Road 107, Guangzhou, Guangdong 510120 , China. zhhosh@126.com
authors.forename: Hongshan
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianpei
authors.initials: J
authors.lastname: Fang
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Wu
authors.forename: Yougang
authors.initials: Y
authors.lastname: Mai
authors.forename: Wenyu
authors.initials: W
authors.lastname: Lai
authors.forename: Haobin
authors.initials: H
authors.lastname: Su
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D051402:Aquaporin 5 / Q000378:metabolism; D001997:Bronchopulmonary Dysplasia / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D058948:Cell Tracking; D002478:Cells, Cultured; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D018496:Hyperoxia / Q000150:complications*; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C496040:Aqp5 protein, rat; D051402:Aquaporin 5; C494828:Bax protein, rat; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2; D042461:Vascular Endothelial Growth Factor A; D051028:bcl-2-Associated X Protein; C467498:vascular endothelial growth factor A, rat; C077063:Sftpc protein, rat; D049452:Green Fluorescent Proteins