title: Cosmetics Europe multi-laboratory pre-validation of the SkinEthic™ reconstituted human corneal epithelium test method for the prediction of eye irritation.
abstract: Cosmetics Europe, The Personal Care Association, known as Colipa before 2012, conducted a program of technology transfer and assessment of Within/Between Laboratory (WLV/BLV) reproducibility of the SkinEthic™ Reconstituted Human Corneal Epithelium (HCE) as one of two human reconstructed tissue eye irritation test methods. The SkinEthic™ HCE test method involves two exposure time treatment procedures - one for short time exposure (10 min - SE) and the other for long time exposure (60 min - LE) of tissues to test substance. This paper describes pre-validation studies of the SkinEthic™ HCE test method (SE and LE protocols) as well as the Eye Peptide Reactivity Assay (EPRA). In the SE WLV study, 30 substances were evaluated. A consistent outcome with respect to viability measurement across all runs was observed with all substances showing an SD of less than 18%. In the LE WLV study, 44 out of 45 substances were consistently classified. These data demonstrated a high level of reproducibility within laboratory for both the SE and LE treatment procedures. For the LE BLV, 19 out of 20 substances were consistently classified between the three laboratories, again demonstrating a high level of reproducibility between laboratories. The results for EPRA WLV and BLV studies demonstrated that all substances analysed were categorised similarly and that the method is reproducible. The SkinEthic™ HCE test method entered into the experimental phase of a formal ECVAM validation program in 2010.
authors.affiliation: L'Oréal Research & Innovation, Aulnay Sous Bois, France. nalapee@rd.loreal.com
authors.forename: N
authors.initials: N
authors.lastname: Alépée
authors.forename: S
authors.initials: S
authors.lastname: Bessou-Touya
authors.forename: J
authors.initials: J
authors.lastname: Cotovio
authors.forename: A
authors.initials: A
authors.lastname: de Smedt
authors.forename: B
authors.initials: B
authors.lastname: de Wever
authors.forename: C
authors.initials: C
authors.lastname: Faller
authors.forename: P
authors.initials: P
authors.lastname: Jones
authors.forename: B
authors.initials: B
authors.lastname: Le Varlet
authors.forename: M
authors.initials: M
authors.lastname: Marrec-Fairley
authors.forename: U
authors.initials: U
authors.lastname: Pfannenbecker
authors.forename: M
authors.initials: M
authors.lastname: Tailhardat
authors.forename: F
authors.initials: F
authors.lastname: van Goethem
authors.forename: P
authors.initials: P
authors.lastname: McNamee
mesh_terms: D000826:Animal Testing Alternatives; D003358:Cosmetics / Q000633:toxicity*; D019573:Epithelium, Corneal / Q000187:drug effects; D005060:Europe; D006801:Humans; D066298:In Vitro Techniques; D007509:Irritants / Q000633:toxicity*; D007753:Laboratories; D015203:Reproducibility of Results; D018581:Technology Transfer; D018675:Toxicity Tests
chemical_list: D003358:Cosmetics; D007509:Irritants