title: PCR-electrospray ionization time-of-flight mass spectrometry: a new tool for the diagnosis of infective endocarditis from heart valves.
abstract: A polymerase chain reaction with an injection of the amplicons in an electrospray ionization mass spectrometry (PCR-ESI-MS) technique was evaluated for the diagnosis of bacterial and yeast pathogens on 13 cardiac valves with suspected endocarditis. At the moment of surgery, 3/13 PCR-ESI-MS results matched with microbiological documentation. Nine PCR-ESI-MS results correlated with Duke's criteria, leukocytes, C-reactive protein and blood cultures before surgery. The PCR-ESI-MS result of the last valve failed to confirm the blood culture result obtained fifteen days before. With speed and accuracy, this method may be useful to assert microbiological identification and adapt treatment.
authors.affiliation: CHU Lille F-59000 Lille France. frederic.wallet@chru-lille.fr
authors.forename: Frédéric
authors.initials: F
authors.lastname: Wallet
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Herwegh
authors.forename: Christophe
authors.initials: C
authors.lastname: Decoene
authors.forename: René J
authors.initials: RJ
authors.lastname: Courcol
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis; D002175:Candida / Q000254:growth & development / Q000302:isolation & purification; D044066:Cardiobacterium / Q000254:growth & development / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000302:isolation & purification; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D006351:Heart Valves / Q000382:microbiology*; D006801:Humans; D008297:Male; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods; D011425:Propionibacterium acnes / Q000254:growth & development / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification; D057787:Staphylococcus lugdunensis / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D002097:C-Reactive Protein