title: Mass spectrometric analysis reveals O-methylation of pyruvate kinase from pancreatic cancer cells.
abstract: Pyruvate kinase (PK) is an important glycolytic enzyme that catalyzes the dephosphorylation of phosphoenolpyruvate to pyruvate. Human PK isozyme M2 (PKM2), a splice variant of M1, is overexpressed in many cancer cells, and PKM2 has been investigated as a potential tumor marker for diagnostic assays and as a target for cancer therapy. To facilitate identification and characterization of PK, we studied the enzyme from pancreatic cancer cells and normal pancreatic duct cells by electrophoresis and mass spectrometry, and identified multiple O-methylated residues from PK. These findings advance our knowledge of the biochemical properties of PK and will be important in understanding its biological function in cells.
authors.affiliation: Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA 20110, USA. wzhou@gmu.edu
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.forename: Michela
authors.initials: M
authors.lastname: Capello
authors.forename: Claudia
authors.initials: C
authors.lastname: Fredolini
authors.forename: Leda
authors.initials: L
authors.lastname: Racanicchi
authors.forename: Erica
authors.initials: E
authors.lastname: Dugnani
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Piemonti
authors.forename: Lance A
authors.initials: LA
authors.lastname: Liotta
authors.forename: Francesco
authors.initials: F
authors.lastname: Novelli
authors.forename: Emanuel F
authors.initials: EF
authors.lastname: Petricoin
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000201:enzymology*; D011770:Pyruvate Kinase / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D011770:Pyruvate Kinase