title: BmCyclin B and BmCyclin B3 are required for cell cycle progression in the silkworm, Bombyx mori.
abstract: Cyclin B is an important regulator of the cell cycle G2 to M phase transition. The silkworm genomic database shows that there are two Cyclin B genes in the silkworm (Bombyx mori), BmCyclin B and BmCyclin B3. Using silkworm EST data, the cyclin B3 (EU074796) gene was cloned. Its complete cDNA was 1665 bp with an ORF of 1536 bp derived from seven exons and six introns. The BmCyclin B3 gene encodes 511 amino acids, and the predicted molecular weight is 57.8 kD with an isoelectric point of 9.18. The protein contains one protein damage box and two cyclin boxes. RNA interference-mediated reduction of BmCyclin B and BmCyclin B3 expression induced cell cycle arrest in G2 or M phase in BmN-SWU1 cells, thus inhibiting cell proliferation. These results suggest that BmCyclin B and BmCyclin B3 are necessary for completing the cell cycle in silkworm cells.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
authors.forename: Minhui
authors.initials: M
authors.lastname: Pan
authors.forename: Kaili
authors.initials: K
authors.lastname: Hong
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Chen
authors.forename: Chun
authors.initials: C
authors.lastname: Pan
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Kuang
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000166:cytology / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation; D003001:Cloning, Molecular; D019926:Cyclin B / Q000145:classification / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D019926:Cyclin B; D019476:Insect Proteins