title: Determination of carbohydrates by high performance anion chromatography-pulsed amperometric detection in mushrooms.
abstract: A method of detecting carbohydrates (fucose, trehalose, mannitol, arabitol, mannose, glucose, galactose, fructose, and ribose) by high-performance anion chromatography-pulsed amperometric detection (HAPEC-PAD) was established. The conditions are: CarboPac MA1 column, NaOH as the eluent, temperature 30°C, Au working electrode, Ag/AgCl reference electrode, and flow rate 0.4 mL/min. These nine analytes, which yielded high resolution by this method, could be detected in 40 minutes. Mushrooms were tested and good precision, stability, and reproducibility were achieved. This method is suitable for mushroom samples and could support research and development on sugar and sugar alcohol, which contains special effects.
authors.affiliation: National Engineering Research Center of Edible Fungi, Shanghai Academy.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhou
authors.forename: Qingjiu
authors.initials: Q
authors.lastname: Tang
authors.forename: Xi
authors.initials: X
authors.lastname: Luo
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Xue
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jingsong
authors.initials: J
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Feng
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D005504:Food Analysis / Q000379:methods
chemical_list: D002241:Carbohydrates