title: Downregulation of miRNA-128 sensitises breast cancer cell to chemodrugs by targeting Bax.
abstract: miR-128 is more highly expressed in drug-resistant breast cancer samples than in drug-sensitive samples. We have confirmed that Bax is the target of miR-128 by negative post-transcriptional regulation. miR-128 and Bax were detected in the breast cancer cell line, MDA-MB-231, which was then transfected with miR-128 MIMIC (precursor of miR-128) or AMO (antisense-miR-128 oligonucleotides). After transfection, the chemosensitivity of MDA-MB-231 cell was up-regulated with increasing of Bax and inhibition of miR-128.
authors.affiliation: Department of General Surgery, Zhujiang Hospital of Nanfang Medical University, No 253 Industrial Avenue, Haizhu District of Guangzhou City, Guangdong Province 501282, China.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Ji
authors.forename: Guoli
authors.initials: G
authors.lastname: Shao
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Lv
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Fan
authors.forename: Aiguo
authors.initials: A
authors.lastname: Wu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Hu
mesh_terms: D000368:Aged; D017209:Apoptosis; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008875:Middle Aged; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: C545189:MIRN128 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D034741:RNA, Small Interfering; D051028:bcl-2-Associated X Protein