title: Ex vivo neurogenesis within enteric ganglia occurs in a PTEN dependent manner.
abstract: A population of multipotent stem cells capable of differentiating into neurons and glia has been isolated from adult intestine in humans and rodents. While these cells may provide a pool of stem cells for neurogenesis in the enteric nervous system (ENS), such a function has been difficult to demonstrate in vivo. An extensive study by Joseph et al. involving 108 rats and 51 mice submitted to various insults demonstrated neuronal uptake of thymidine analog BrdU in only 1 rat. Here we introduce a novel approach to study neurogenesis in the ENS using an ex vivo organotypic tissue culturing system. Culturing longitudinal muscle and myenteric plexus tissue, we show that the enteric nervous system has tremendous replicative capacity with the majority of neural crest cells demonstrating EdU uptake by 48 hours. EdU(+) cells express both neuronal and glial markers. Proliferation appears dependent on the PTEN/PI3K/Akt pathway with decreased PTEN mRNA expression and increased PTEN phosphorylation (inactivation) corresponding to increased Akt activity and proliferation. Inhibition of PTEN with bpV(phen) augments proliferation while LY294002, a PI3K inhibitor, blocks it. These data suggest that the ENS is capable of neurogenesis in a PTEN dependent manner.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, California, United States of America.
authors.forename: Laren
authors.initials: L
authors.lastname: Becker
authors.forename: Johann
authors.initials: J
authors.lastname: Peterson
authors.forename: Subhash
authors.initials: S
authors.lastname: Kulkarni
authors.forename: Pankaj Jay
authors.initials: PJ
authors.lastname: Pasricha
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D003433:Crosses, Genetic; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D017615:Enteric Nervous System / Q000254:growth & development*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005724:Ganglia / Q000254:growth & development*; D051379:Mice; D008856:Microscopy, Fluorescence; D009432:Neural Crest / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003856:Deoxyuracil Nucleotides; C027383:2'-deoxy-5-ethyl-5'-uridylic acid; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse