title: [Biomechanics of cartilage tissue engineering constructs : Sensitive test procedure for assessment of biomechanical functionality and further development after in vivo transplantation].
abstract: Specific biomechanical properties represent important quality markers of cartilage tissue engineering (TE) constructs. The aim of the study was to identify a sensitive biomechanical test to assess mechanical properties of cartilage TE constructs. Biomechanical testing of in vitro cultivated constructs following the very low rubber hardness (VLRH) principle illustrated significant differences between constructs cultured under chondrogenic conditions over various periods of time. An increase in proteoglycan and collagen type II deposition corresponded to increasing VLRH hardness values. Although a decrease in proteoglycan was detected after ectopic implantation of constructs into SCID mice, no reduction in biomechanical hardness values was observed. A functional estimation of TE constructs requires determination of biomechanical and biochemical parameters as quality features.
authors.affiliation: Forschungszentrum für Experimentelle Orthopädie, Stiftung Orthopädische Universitätsklinik Heidelberg, Schlierbacher Landstr. 200a, 69118 Heidelberg, Deutschland.
authors.forename: A
authors.initials: A
authors.lastname: Krase
authors.forename: E
authors.initials: E
authors.lastname: Steck
authors.forename: W
authors.initials: W
authors.lastname: Roth
authors.forename: W
authors.initials: W
authors.lastname: Richter
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D004867:Equipment Design; D019544:Equipment Failure Analysis / Q000379:methods; D050724:Fractures, Cartilage / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006801:Humans; D008422:Materials Testing / Q000379:methods; D012038:Regeneration / Q000502:physiology*; D013552:Swine; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials