title: Is myeloperoxidase a key component in the ROS-induced vascular damage related to nephropathy in type 2 diabetes?
abstract: It is still unclear whether microvascular complications of type 2 diabetes correlate with leukocyte-endothelium interactions and/or myeloperoxidase (MPO) levels. In the present study, we found that serum levels of glucose, the rate of ROS and MPO concentration were higher in type 2 diabetic patients. Patients with nephropathy (39.6%) presented higher MPO levels that correlate positively with the albumin/creatinine ratio (r = 0.59, p<0.05). In addition, nephropatic patients showed increased leukocyte-endothelium interactions due to an undermining of polymorphonuclear leukocytes (PMN) rolling velocity and increased rolling flux and adhesion, which was accompanied by a rise in levels of the proinflammatory cytokine tumour necrosis factor alpha (TNFα) and the adhesion molecule E-selectin. Furthermore, MPO levels were positively correlated with PMN rolling flux (r = 0.855, p < 0.01) and adhesion (r = 0.682, p<0.05). Our results lead to the hypothesis that type 2 diabetes induces oxidative stress and an increase in MPO levels and leukocyte-endothelium interactions, and that these effects correlate with the development of nephropathy.
authors.affiliation: 1 Fundacion para la Investigacion Sanitaria y Biomedica de la Comunidad Valenciana FISABIO, Endocrinology Service, University Hospital Doctor Peset , Valencia, Spain .
authors.forename: Susana
authors.initials: S
authors.lastname: Rovira-Llopis
authors.forename: Milagros
authors.initials: M
authors.lastname: Rocha
authors.forename: Rosa
authors.initials: R
authors.lastname: Falcon
authors.forename: Carmen
authors.initials: C
authors.lastname: de Pablo
authors.forename: Angeles
authors.initials: A
authors.lastname: Alvarez
authors.forename: Ana
authors.initials: A
authors.lastname: Jover
authors.forename: Antonio
authors.initials: A
authors.lastname: Hernandez-Mijares
authors.forename: Victor M
authors.initials: VM
authors.lastname: Victor
mesh_terms: D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000378:metabolism* / Q000473:pathology*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D008875:Middle Aged; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D017382:Reactive Oxygen Species; D009195:Peroxidase