title: Nanog is an essential factor for induction of pluripotency in somatic cells from endangered felids.
abstract: Nanog has an important role in pluripotency induction in bovines and snow leopards. To examine whether it was required for wild felids globally, we examined the induction of pluripotency in felids from Asia (Bengal tiger, Panthera tigris), Africa (serval, Leptailurus serval), and the Americas (jaguar, Panthera onca). Dermal fibroblasts were transduced with genes encoding the human transcription factors OCT4, SOX2, KLF4, and cMYC with or without NANOG. Both four- and five-factor induction resulted in colony formation at day 3 in all three species tested; however, we were not able to maintain colonies that were generated without NANOG beyond passage (P) 7. Five-factor induced pluripotent stem cell (iPSC) colonies from wild cats were expanded in vitro on feeder layers and were positive for alkaline phosphatase and protein expression of OCT-4, NANOG, and stage-specific embryonic antigen-4 at P4 and P14. Reverse-transcription polymerase chain reaction confirmed that all five human transgenes were transcribed at P4; however, OCT4, SOX2, and NANOG transgenes were silenced by P14. Endogenous OCT4 and NANOG transcripts were detected at P4 and P14 in all cell lines confirming successful reprogramming. At P14, the iPSCs from all three species remained euploid and differentiated in vivo and in vitro into derivatives of the three germ layers. This study describes an effective method for inducing pluripotency in three endangered wild cats from across the globe and confirms Nanog as an essential factor in the reprogramming event. Efficient production of iPSC from endangered felids creates a unique opportunity for species preservation through gamete production, nuclear transfer, embryo complementation, and future novel technologies.
authors.affiliation: Center for Reproduction and Development, Monash Institute of Medical Research, Monash University , Clayton, Australia .
authors.forename: Rajneesh
authors.initials: R
authors.lastname: Verma
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Michael Kenneth
authors.initials: MK
authors.lastname: Holland
authors.forename: Peter
authors.initials: P
authors.lastname: Temple-Smith
authors.forename: Mark
authors.initials: M
authors.lastname: Williamson
authors.forename: Paul John
authors.initials: PJ
authors.lastname: Verma
keywords: Nanog; conservation; induced pluripotent stem cells; wild cats