title: [Preparation of salbutamol polyclonal antibodies and development of indirect competitive enzyme-linked immunoassay].
abstract: OBJECTIVE
  To prepare the antibodies against salbutamol (SAL) with high sensitivity and to develop an indirect competitive enzyme-linked immunoassay (ic-ELISA) for fast detection of SAL.
  
  
  METHODS
  The New Zealand white rabbits were immunized with SAL in a small dose and long period mode. The method of ic-ELISA was optimized and adopted for the detection of a series of SAL samples, then the standard curve of SAL was established. The precision and the recoveries of the method were determined.
  
  
  RESULTS
  The antibodies with high sensitivity towards SAL were prepared with a IC50 of 12.21 ng/ml. The ic-ELISA method for SAL measurement was established, the recoveries of measurement was between 95%-105% and the CV was <3%.
  
  
  CONCLUSIONS
  The antibodies against salbutamol have been prepared and an indirect competitive enzyme-linked immunoassay for fast and specific detection of SAL has been developed.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Zhang
authors.forename: Yajing
authors.initials: Y
authors.lastname: Lei
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Xu
authors.forename: Weixing
authors.initials: W
authors.lastname: Shi
authors.forename: Shuqing
authors.initials: S
authors.lastname: Chen
mesh_terms: D000420:Albuterol / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008297:Male; D011817:Rabbits
chemical_list: D000906:Antibodies; D000420:Albuterol