title: Deficiency in origin licensing proteins impairs cilia formation: implications for the aetiology of Meier-Gorlin syndrome.
abstract: Mutations in ORC1, ORC4, ORC6, CDT1, and CDC6, which encode proteins required for DNA replication origin licensing, cause Meier-Gorlin syndrome (MGS), a disorder conferring microcephaly, primordial dwarfism, underdeveloped ears, and skeletal abnormalities. Mutations in ATR, which also functions during replication, can cause Seckel syndrome, a clinically related disorder. These findings suggest that impaired DNA replication could underlie the developmental defects characteristic of these disorders. Here, we show that although origin licensing capacity is impaired in all patient cells with mutations in origin licensing component proteins, this does not correlate with the rate of progression through S phase. Thus, the replicative capacity in MGS patient cells does not correlate with clinical manifestation. However, ORC1-deficient cells from MGS patients and siRNA-mediated depletion of origin licensing proteins also have impaired centrosome and centriole copy number. As a novel and unexpected finding, we show that they also display a striking defect in the rate of formation of primary cilia. We demonstrate that this impacts sonic hedgehog signalling in ORC1-deficient primary fibroblasts. Additionally, reduced growth factor-dependent signaling via primary cilia affects the kinetics of cell cycle progression following cell cycle exit and re-entry, highlighting an unexpected mechanism whereby origin licensing components can influence cell cycle progression. Finally, using a cell-based model, we show that defects in cilia function impair chondroinduction. Our findings raise the possibility that a reduced efficiency in forming cilia could contribute to the clinical features of MGS, particularly the bone development abnormalities, and could provide a new dimension for considering developmental impacts of licensing deficiency.
authors.affiliation: Double Strand Break Repair Laboratory, Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors.forename: Tom
authors.initials: T
authors.lastname: Stiff
authors.forename: Meryem
authors.initials: M
authors.lastname: Alagoz
authors.forename: Diana
authors.initials: D
authors.lastname: Alcantara
authors.forename: Emily
authors.initials: E
authors.lastname: Outwin
authors.forename: Han G
authors.initials: HG
authors.lastname: Brunner
authors.forename: Ernie M H F
authors.initials: EM
authors.lastname: Bongers
authors.forename: Mark
authors.initials: M
authors.lastname: O'Driscoll
authors.forename: Penny A
authors.initials: PA
authors.lastname: Jeggo
mesh_terms: D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000235:genetics; D002502:Centrioles / Q000235:genetics / Q000378:metabolism; D002923:Cilia / Q000235:genetics / Q000502:physiology; D065817:Congenital Microtia; D004261:DNA Replication / Q000235:genetics*; D004392:Dwarfism / Q000235:genetics*; D004423:Ear / Q000002:abnormalities; D019066:Facies; D006130:Growth Disorders / Q000209:etiology / Q000235:genetics*; D006801:Humans; D008831:Microcephaly / Q000235:genetics*; D008844:Micrognathism / Q000209:etiology / Q000235:genetics*; D051738:Origin Recognition Complex / Q000235:genetics*; D010329:Patella / Q000002:abnormalities; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D016196:S Phase / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; C497223:ORC1 protein, human; D051738:Origin Recognition Complex; C576597:ATR protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases