title: In vivo gene delivery with L-tyrosine polyphosphate nanoparticles.
abstract: The concept of gene therapy is promising; however, the perceived risks and side effects associated with this technology have severely dampened the researchers' enthusiasm. Thus, the development of a nonviral gene vector without immunological effects and with high transfection efficiency is necessary. Currently, most nonviral vectors have failed to achieve the in vivo transfection efficiencies of viral vectors due to their toxicity, rapid clearance, and/or inappropriate release rates. Although our previous studies have successfully demonstrated the controlled-release of plasmid DNA (pDNA) polyplexes encapsulated into nanoparticles formulated with l-tyrosine polyphosphate (LTP-pDNA nanoparticles), the in vivo transfection capabilities and immunogenicity of this delivery system have yet to be examined. Thus, we evaluate LTP-pDNA nanoparticles in an in vivo setting via injection into rodent uterine tissue. Our results demonstrate through X-gal staining and immunohistochemistry of uterine tissue that transfection has successfully occurred after a nine-day incubation. In contrast, the results for the control nanoparticles show results similar to those of shams. Furthermore, reverse transcriptase polymerase chain reaction (RT-PCR) from the injected tissues confirms the transfection in vivo. To examine the immunogenicity, the l-tyrosine polyphosphate (LTP) nanoparticles have been evaluated in a mouse model. No significant differences in the activation of the innate immune system are observed. These data provide the first report for the potential use of controlled-release nanoparticles formulated from an amino acid based polymer as an in vivo nonviral vector for gene therapy.
authors.affiliation: Department of Biomedical Engineering, The University of Akron, Olson Research Center, Akron, Ohio 44325-0302, United States.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Ditto
authors.forename: John J
authors.initials: JJ
authors.lastname: Reho
authors.forename: Kush N
authors.initials: KN
authors.lastname: Shah
authors.forename: Justin A
authors.initials: JA
authors.lastname: Smolen
authors.forename: James H
authors.initials: JH
authors.lastname: Holda
authors.forename: Rolando J
authors.initials: RJ
authors.lastname: Ramirez
authors.forename: Yang H
authors.initials: YH
authors.lastname: Yun
mesh_terms: D000818:Animals; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D007113:Immunity, Innate; D051379:Mice; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010755:Organophosphates / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*; D011921:Rats, Inbred WKY; D014162:Transfection; D014599:Uterus / Q000276:immunology / Q000378:metabolism
chemical_list: D053769:Nanocapsules; D010755:Organophosphates; D011108:Polymers; C542009:tyrosine polyphosphate