title: Development of immunoassays for detecting clothianidin residue in agricultural products.
abstract: Two enzyme-linked immunosorbent assays (ELISAs) based on polyclonal antibodies (PcAbs) for clothianidin are described: colorimetric detection format (ELISA) and pattern of chemiluminescent assay (CLEIA). Clothianidin hapten was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to produce immunogen and coating antigen. Anticlothianidin PcAbs were obtained from immunized New Zealand white rabbits. Under optimal conditions, the half-maximal inhibition concentration (IC₅₀) and the limit of detection (LOD, IC₂₀) of clothianidin were 0.046 and 0.0028 mg/L for the ELISA and 0.015 and 0.0014 mg/L for the CLEIA, respectively. There were no obvious cross-reactivities of the antibodies with its analogues except for dinotefuran. Recoveries of 76.4-116.4% for the immunoassays were achieved from spiked samples. The results of immunoassays for the spiked and authentic samples were largely consistent with gas chromatography. Therefore, the proposed immunoassays would be convenient and satisfactory analytical methods for the monitoring of clothianidin in agricultural products.
authors.affiliation: Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Ministry of Education, Nanjing 210095, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Enze
authors.initials: E
authors.lastname: Sheng
authors.forename: Lujing
authors.initials: L
authors.lastname: Cong
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
mesh_terms: D002681:China; D018556:Crops, Agricultural / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D060587:Groundwater; D006146:Guanidines / Q000032:analysis* / Q000378:metabolism; D007306:Insecticides / Q000032:analysis* / Q000378:metabolism; D000073943:Neonicotinoids; D010573:Pesticide Residues / Q000032:analysis* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism; D013844:Thiazoles / Q000032:analysis* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism
chemical_list: D006146:Guanidines; D007306:Insecticides; D000073943:Neonicotinoids; D010573:Pesticide Residues; D012989:Soil Pollutants; D013844:Thiazoles; D014874:Water Pollutants, Chemical; C480342:clothianidin