title: A Template-Mediated Click-Click Reaction: PNA-DNA, PNA-PNA (or Peptide) Ligation, and Single Nucleotide Discrimination.
abstract: A highly efficient chemical ligation was developed for quantitative conjugation of PNA with DNA (PNA or peptide) using the copper-catalyzed azide-alkyne cycloaddition reaction. While PNAs with an alkyne at the C-terminus and an azide at the N-terminus have been used, an efficient click-click reaction occurs. The PNA click ligation is sequence-specific and capable of single nucleotide discrimination.
authors.affiliation: Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 3210 N. Cramer St., Milwaukee, 53211, U.S.A.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Michael
authors.initials: M
authors.lastname: Seidman
keywords: Chemical Ligation; Click Chemistry; DNA detection; Peptide Nucleic Acids; Single Nucleotide Discrimination