title: Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan.
abstract: An unusual rotavirus strain, Ryukyu-1120, with G5P[6] genotypes (RVA/Human-wt/JPN/Ryukyu-1120/2011/G5P[6]) was identified in a stool specimen from a hospitalized child aged 4 years who showed diarrhoea and encephalopathy. In this study, we sequenced and characterized the complete genome of strain Ryukyu-1120. On whole genomic analysis, this strain was found to have a unique genotype constellation: G5-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The VP6 and NSP1 genotypes I5 and A8 are those commonly found in porcine strains. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain Ryukyu-1120 appeared to be of porcine origin. Thus, strain Ryukyu-1120 was found to have a porcine rotavirus genetic backbone and is likely to be of porcine origin. To our knowledge, this is the first report of whole-genome-based characterization of the emerging G5P[6] strains in Asian countries. Our observations will provide important insights into the origin of G5P[6] strains and the dynamic interactions between human and porcine rotavirus strains.
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komoto
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Maeno
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Mayuko
authors.initials: M
authors.lastname: Tomita
authors.affiliation: Division of Pediatric Neurology and General, Okinawa Prefectural Nanbu Medical Center and Children's Medical Center, Haebaru-cho, Okinawa 901-1193, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Matsuoka
authors.affiliation: Division of Pediatric Neurology and General, Okinawa Prefectural Nanbu Medical Center and Children's Medical Center, Haebaru-cho, Okinawa 901-1193, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ohfu
authors.affiliation: Division of Pediatric Neurology and General, Okinawa Prefectural Nanbu Medical Center and Children's Medical Center, Haebaru-cho, Okinawa 901-1193, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Yodoshi
authors.affiliation: Division of Pediatric Neurology and General, Okinawa Prefectural Nanbu Medical Center and Children's Medical Center, Haebaru-cho, Okinawa 901-1193, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Akeda
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D003967:Diarrhea / Q000821:virology*; D018792:Encephalitis, Viral / Q000821:virology*; D005243:Feces; D016679:Genome, Viral / Q000235:genetics*; D023281:Genomics / Q000379:methods; D005838:Genotype; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology; D017422:Sequence Analysis, DNA; D013552:Swine / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C061332:VP6 protein, Rotavirus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C104282:nsp1 protein, Rotavirus