title: High-yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell-free system.
abstract: Let enzymes work: H2 was produced from xylose and water in one reactor containing 13 enzymes (red). By using a novel polyphosphate xylulokinase (XK), xylose was converted into H2 and CO2 with approaching 100 % of the theoretical yield. The findings suggest that cell-free biosystems could produce H2 from biomass xylose at low cost. Xu5P = xylulose 5-phosphate, G6P = glucose 6-phosphate.
authors.affiliation: Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Julia S
authors.initials: JS
authors.lastname: Martín del Campo
authors.forename: Joseph
authors.initials: J
authors.lastname: Rollin
authors.forename: Suwan
authors.initials: S
authors.lastname: Myung
authors.forename: You
authors.initials: Y
authors.lastname: Chun
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Chandrayan
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Patiño
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: Y-H Percival
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000737:chemistry; D019747:Aldose-Ketose Isomerases / Q000737:chemistry; D055162:Biocatalysis; D002474:Cell-Free System / Q000737:chemistry* / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry; D014994:Xylose / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D006859:Hydrogen; D014994:Xylose; D006864:Hydrogenase; D017766:Acid Anhydride Hydrolases; C022563:endopolyphosphatase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase