title: A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.
abstract: Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis.
authors.affiliation: University Paris-Est, Anses, Animal Health Laboratory, Bacterial Zoonoses Unit, Maisons-Alfort, France.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Zocevic
authors.forename: Fabien
authors.initials: F
authors.lastname: Vorimore
authors.forename: Nadia
authors.initials: N
authors.lastname: Vicari
authors.forename: Julien
authors.initials: J
authors.lastname: Gasparini
authors.forename: Lisa
authors.initials: L
authors.lastname: Jacquin
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.forename: Simone
authors.initials: S
authors.lastname: Magnino
authors.forename: Karine
authors.initials: K
authors.lastname: Laroucau
mesh_terms: D000818:Animals; D002693:Chlamydiaceae / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D010856:Columbidae / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D004269:DNA, Bacterial; D010751:Phosphopyruvate Hydratase