title: Biochemical and electrophoretic analyses of saliva from the predatory reduviid species Rhynocoris marginatus (Fab.).
abstract: The saliva of Rhynocoris marginatus consists of amylase, invertase, trehalase, protease, acid phosphatase, alkaline phosphatase, phospholipase, lipase, trypsin, hyaluronidase, and esterase. All enzyme activities were significantly higher in the saliva of female R. marginatus when compared to the saliva of male individuals. The saliva was analyzed by tricine SDS/PAGE, sephadex column chromatography, FT-IR, and MALDI-TOF. The pH of the saliva was slightly alkali. The SDS/PAGE revealed a few proteins with molecular masses greater than 29.5 and 36.2 kDa for male and female predator saliva respectively. The FT-IR spectrum confirmed the acidic, proteinaceous, enzymatic, and aromatic nature of the saliva. The MALDI-TOF-MS revealed the presence of enzymes, proteins, peptides, and other biomolecules. The most prominent peptides were named as RmIT-1 (3.79 kDa), RmIT-2 (9.7 kDa), and RmIT-3 (10.94 kDa) (Rhynocoris marginatus Insect Toxin). Further studies are underway to isolate and identify these biomolecules.
authors.affiliation: Crop Protection Research Centre, Department of Advanced Zoology and Biotechnology, St. Xavier's College (Autonomous), Palayamkottai, Tamil Nadu, India. ksraj42@gmail.com
authors.forename: Sahayaraj
authors.initials: S
authors.lastname: Kitherian
authors.forename: Subramanium
authors.initials: S
authors.lastname: Muthukumar
authors.forename: David
authors.initials: D
authors.lastname: Rivers
mesh_terms: D000818:Animals; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D020064:Reduviidae / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared