title: Draft Genome Sequence of Escherichia coli Strain Nissle 1917 (Serovar O6:K5:H1).
abstract: We announce the availability of the 5.023-Mbp high-quality draft assembly of the Escherichia coli strain Nissle 1917 (serovar O6:K5:H1) genome. Short genomic segments from this important probiotic strain have been available in public databases, but the full genome sequence has remained inaccessible. Thus, high-coverage, whole genome sequencing of E. coli Nissle 1917 is presented herein. Reannotation and metabolic reconstruction will enable comparative genomics analysis and model-guided predictions of genetic manipulations leading to increased production of the K5 capsular polysaccharide known as N-acetyl heparosan, a precursor to the anticoagulant pharmaceutical heparin.
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York, USA.
authors.forename: Brady F
authors.initials: BF
authors.lastname: Cress
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
authors.forename: Mattheos A G
authors.initials: MA
authors.lastname: Koffas