title: Genomic organization, phylogenetic comparison and differential expression of the SBP-box family genes in grape.
abstract: BACKGROUND
  The SBP-box gene family is specific to plants and encodes a class of zinc finger-containing transcription factors with a broad range of functions. Although SBP-box genes have been identified in numerous plants including green algae, moss, silver birch, snapdragon, Arabidopsis, rice and maize, there is little information concerning SBP-box genes, or the corresponding miR156/157, function in grapevine.
  
  
  RESULTS
  Eighteen SBP-box gene family members were identified in Vitis vinifera, twelve of which bore sequences that were complementary to miRNA156/157. Phylogenetic reconstruction demonstrated that plant SBP-domain proteins could be classified into seven subgroups, with the V. vinifera SBP-domain proteins being more closely related to SBP-domain proteins from dicotyledonous angiosperms than those from monocotyledonous angiosperms. In addition, synteny analysis between grape and Arabidopsis demonstrated that homologs of several grape SBP genes were found in corresponding syntenic blocks of Arabidopsis. Expression analysis of the grape SBP-box genes in various organs and at different stages of fruit development in V. quinquangularis 'Shang-24' revealed distinct spatiotemporal patterns. While the majority of the grape SBP-box genes lacking a miR156/157 target site were expressed ubiquitously and constitutively, most genes bearing a miR156/157 target site exhibited distinct expression patterns, possibly due to the inhibitory role of the microRNA. Furthermore, microarray data mining and quantitative real-time RT-PCR analysis identified several grape SBP-box genes that are potentially involved in the defense against biotic and abiotic stresses.
  
  
  CONCLUSIONS
  The results presented here provide a further understanding of SBP-box gene function in plants, and yields additional insights into the mechanism of stress management in grape, which may have important implications for the future success of this crop.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Hou
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Stacy D
authors.initials: SD
authors.lastname: Singer
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Linyong
authors.initials: L
authors.lastname: Mao
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Fei
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D040481:Genome Components / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D026801:Synteny / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D027843:Vitis / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D014157:Transcription Factors