title: An applicable strategy for improvement recovery in simultaneous analysis of 20 pesticides residue in tea.
abstract: It is important to have a reliable method to analyze pesticides in tea, a beverage commonly consumed in Iran. A validated method was developed for the determination of 20 pesticides in tea based on QuEChERS sample preparation and capillary gas chromatography-quadrupole mass spectrometry in selective ion monitoring mode (GC-MS/SIM) using triphenyl methane (TPM) solution as an internal standard. We used fortified, extracted, and cleaned-up tea samples instead of calibration standards for quantitation, which substantially reduced adverse matrix-related effects and negative recovery affected by graphite carbon black (GCB) on pesticide analysis. The recovery of pesticides at 3 concentration (40, 60, and 240 ng/g) ranged from 79.5% to 111.4% (n = 3). The method had acceptable repeatability with RSDr < 20%. The limits of quantification (LOQ) for all pesticides were ≤20 ng/g. The analytical results of the proposed method were in good agreement with proficiency test results (FAPAS, 19116). The recoveries and repeatabilities were in accordance with the criteria set by SANCO Guideline. The validated method was suitable for the analysis of pesticides in tea.
authors.affiliation: Food and Drug Laboratory Research Center and Food and Drug Reference Control Laboratories Center, Food and Drug Organization, MOH & ME, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Shoeibi
authors.forename: Maryam
authors.initials: M
authors.lastname: Amirahmadi
authors.forename: Hossein
authors.initials: H
authors.lastname: Rastegar
authors.forename: Roya
authors.initials: R
authors.lastname: Khosrokhavar
authors.forename: Amin Mousavi
authors.initials: AM
authors.lastname: Khaneghah
mesh_terms: D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D007492:Iran; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013662:Tea / Q000737:chemistry*
chemical_list: D010573:Pesticide Residues; D013662:Tea