title: Numerical study of cell cryo-preservation: a network model of intracellular ice formation.
abstract: In this study, a new intracellular ice formation network model, coupled with an improved cell dehydration model has been developed. The non-uniform dehydration of the cell during freezing is simulated with moving boundary condition. Internal cell structures like cell nucleus are taken into consideration. The IIF network model is developed from classic diffusion limited IIF model in order to simulate spatial ice growth pattern inside cells. Simulation results suggest that cell nuclear plays a significant role in cryo-dehydration and would affect water/CPA concentration gradient inside the cell. At the same time, the ice growth pattern of exogenous IIF hypothesis is examined in the model. It is consistent with our previous experiments, in which we witnessed the intracellular ice first grown into the nucleus before spreading to the whole intercellular space. According to this model, the water concentration difference between nucleus and cytoplasm during cryo-dehydration could partly explain why ice crystal in the nucleus grows faster. However, it is not the dominate factor. Higher diffusion coefficient in cell nucleus might play a more important role in the phenomenon.
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Geer
authors.initials: G
authors.lastname: Yang
authors.forename: Aili
authors.initials: A
authors.lastname: Zhang
authors.forename: Lisa X
authors.initials: LX
authors.lastname: Xu
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D007053:Ice; D007424:Intracellular Fluid / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D007053:Ice