title: Development of a "signal-on" electrochemical DNA sensor with an oligo-thymine spacer for point mutation detection.
abstract: We report a reagentless and reusable "signal-on" electrochemical DNA sensor that utilizes oligo-thymine as a flexible spacer. This sensor design is sensitive, specific, and selective; it is potentially generalizable for detection of a wide range of biomedical-relevant nucleic acid sequences.
authors.affiliation: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.forename: Rebecca Y
authors.initials: RY
authors.lastname: Lai
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008751:Methylene Blue / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D017354:Point Mutation; D013941:Thymine / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D009841:Oligonucleotides; D006046:Gold; D004247:DNA; D013941:Thymine; D008751:Methylene Blue