title: Optimisation of the hydrolysis of goat milk protein for the production of ACE-inhibitory peptides.
abstract: Goat milk protein was hydrolysed with subtilisin and trypsin. As input variables, temperature was assayed in the interval 45-70 °C for subtilisin and 30-55 °C for trypsin, while the enzyme-substrate ratio varied from 1 to 5%. The effect of the input variables on the degree of hydrolysis and ACE-inhibitory activity (output variables) was modelled by second order polynomials, which were able to fit the experimental data with deviations below 10%. The individual maximum values of the degree of hydrolysis and the ACE-inhibitory activity were found at conflicting conditions of temperature and enzyme-substrate ratio. Since such maximum values could not be reached simultaneously, a bi-objective optimisation procedure was undertaken, producing a set of non-inferior solutions that weighted both objectives.
authors.affiliation: Department of Chemical Engineering, University of Granada, 18071 Granada, Spain. fjespejo@ugr.es
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Espejo-Carpio
authors.forename: Raúl
authors.initials: R
authors.lastname: Pérez-Gálvez
authors.forename: Emilia M
authors.initials: EM
authors.lastname: Guadix
authors.forename: Antonio
authors.initials: A
authors.lastname: Guadix
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D006041:Goats; D006868:Hydrolysis; D008894:Milk Proteins / Q000378:metabolism* / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D020860:Subtilisin / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D008894:Milk Proteins; D010455:Peptides; D014357:Trypsin; D020860:Subtilisin