title: Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli.
abstract: Octaprenyl pyrophosphate synthase (OPPs), which belongs to the E-type prenyltransferase family, catalyses the successive condensation of farnesyl pyrophosphate with five isopentenyl pyrophosphate molecules to form trans-C40-octaprenyl pyrophosphate (OPP). OPP is essential for the biosynthesis of bacterial ubiquinone or menaquinone side chains, which play an important role in the electron-transport system. Here, Escherichia coli OPPs was expressed, purified and crystallized. The crystals, which belonged to the orthorhombic space group P2₁2₁2, with unit-cell parameters a=117.0, b=128.4, c=46.4 Å, were obtained by the sitting-drop vapour-diffusion method and diffracted to 2.2 Å resolution. Initial phase determination by molecular replacement (MR) clearly indicated that the crystal contained one homodimer per asymmetric unit. Further model building and structural refinement are in progress.
authors.affiliation: Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.forename: Tzu-Ping
authors.initials: TP
authors.lastname: Ko
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Erbing
authors.initials: E
authors.lastname: Hua
authors.forename: Rey-Ting
authors.initials: RT
authors.lastname: Guo
authors.forename: Chun-Hsiang
authors.initials: CH
authors.lastname: Huang
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D019883:Alkyl and Aryl Transferases; C048987:octaprenyl pyrophosphate synthetase
keywords: Escherichia coli; octaprenyl pyrophosphate; octaprenyl pyrophosphate synthase; prenyltransferase