title: Mitigated cytotoxicity and tremendously enhanced gene transfection efficiency of PEI through facile one-step carbamate modification.
abstract: Extremely efficacious gene transfection vector: The rapid and facile modification of PEI with commercially available TMC produces an extremely efficacious gene delivery vector with minimal cytotoxicity. Functionalization of PEI is easily controlled by PEI:cyclic carbonate feed ratios and allows for the addition of functionality. Modified PEIs hold great potential as gene delivery systems due to easy synthesis, scalability, low cost, low toxicity, and outstanding transfection capacity.
authors.affiliation: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore.
authors.forename: Chuan
authors.initials: C
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Cheng
authors.forename: Pei Yun
authors.initials: PY
authors.lastname: Teo
authors.forename: Amanda C
authors.initials: AC
authors.lastname: Engler
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Coady
authors.forename: James L
authors.initials: JL
authors.lastname: Hedrick
authors.forename: Yi Yan
authors.initials: YY
authors.lastname: Yang
mesh_terms: D002219:Carbamates / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004146:Dioxanes / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry / Q000633:toxicity; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
chemical_list: D002219:Carbamates; D004146:Dioxanes; C443643:trimethylene carbonate; D011094:Polyethyleneimine; D004247:DNA
keywords: PEI; cyclic carbonate; high gene transfection; negligible toxicity; simple click modification