title: Genetic variability among Dicrocoelium dendriticum isolates from different regions in Shaanxi Province, China revealed by sequences of three mitochondrial genes.
abstract: The genetic variations in three mitochondrial DNA (mtDNA) regions, namely portion of cytochrome c oxidase subunit 1 (pcox1), NADH dehydrogenase subunit 1 (pnad1) and cytochrome b (pcytb), were examined for Dicrocoelium dendriticum samples isolated from different origins in Shaanxi Province, northwestern China. The intra-specific sequence differences within D. dendriticum samples were 0-0.52% for pcox1, 0-0.73% for pnad1 and 0-0.58% for pcytb. Phylogenetic analyses based on combined sequences of three mtDNA showed that all D. dendriticum samples were clustered together in same clade of Paragonimus westermani. But the phylogenetic trees could not reveal geographically genetic relationships of D. dendriticum isolates in this province. These findings will provide basic information for further study of molecular epidemiology and control of D. dendriticum infection in this province as well as in China.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling , Shaanxi Province 712100 , People's Republic of China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhao
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Bian
authors.forename: Wan-Xin
authors.initials: WX
authors.lastname: Ren
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Jia
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Cheng
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Fang
authors.forename: Jun-Ke
authors.initials: JK
authors.lastname: Song
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D010802:Phylogeny; D010986:Platyhelminths / Q000145:classification / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial