title: Membrane defects enhance the interaction of antimicrobial peptides, aurein 1.2 versus caerin 1.1.
abstract: The membrane interactions of the antimicrobial peptides aurein 1.2 and caerin 1.1 were observed by (31)P and (2)H solid-state NMR and circular dichroism spectroscopy. Both peptides were relatively unstructured in water. In the presence of dimyristoylphosphatidylcholine (DMPC) and mixed DMPC and dimyristoylphosphatidylglycerol (DMPG) vesicles, both peptides displayed a considerable increase in helical content with the shorter aurein peptide having a higher α-helix content in both lipid systems. In fluid phase DMPC vesicles, the peptides displayed differential interactions: aurein 1.2 interacted primarily with the bilayer surface, while the longer caerin 1.1 was able to penetrate into the bilayer interior. Both peptides displayed a preferential interaction with the DMPG component in DMPC/DMPG bilayers, with aurein 1.2 limited to interaction with the surface and caerin 1.1 able to penetrate into the bilayer and promote formation of a mixture of lipid phases or domains. In gel phase DMPC vesicles, aurein 1.2 disrupted the bilayer apparently through a carpet mechanism, while no additional interaction was seen with caerin 1.1. Although a lamellar bilayer was retained with the mixed DMPC/DMPG vesicles below the phase transition, both caerin 1.1 and aurein 1.2 promoted disruption of the bilayer and formation of an isotropic phase. The peptide interaction was enhanced relative to the fluid phase and was likely driven by co-existence of membrane defects. This study thus demonstrates that the effects of the lipid phase and domains need to be considered when studying membrane interactions of antimicrobial peptides.
authors.affiliation: School of Chemistry, University of Melbourne, VIC 3010, Australia.
authors.forename: David I
authors.initials: DI
authors.lastname: Fernandez
authors.forename: Marc-Antoine
authors.initials: MA
authors.lastname: Sani
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Miles
authors.forename: B A
authors.initials: BA
authors.lastname: Wallace
authors.forename: Frances
authors.initials: F
authors.lastname: Separovic
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000473:pathology*; D002942:Circular Dichroism; D008051:Lipid Bilayers / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D044367:Phase Transition; D012724:Sex Attractants / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; D010446:Peptide Fragments; D012724:Sex Attractants; C506635:aurein 1.2 peptide; C121856:splendipherin