title: Enhanced replication of R5 HIV-1 isolates in vitro by a small-molecule reagent targeting HIV-1 protease.
abstract: CHEMICAL ENHANCEMENT: Designed to target HIV-1 protease, a novel γ-hydroxyphosphonate has been found to significantly enhance viral replication in a panel of clinically relevant R5 HIV-1 isolates. This unexpected result constitutes the first instance of a small molecule capable of doing this, and it has implications for the preparation and use of R5 isolates in vaccine and drug development.
authors.affiliation: Institute for Health Research and Policy, London Metropolitan University, 166-220 Holloway Rd, London N7 8DB, UK. n.wardle@londonmet.ac.uk
authors.forename: Nick J
authors.initials: NJ
authors.lastname: Wardle
authors.forename: Harry R
authors.initials: HR
authors.lastname: Hudson
authors.forename: Ray W
authors.initials: RW
authors.lastname: Matthews
authors.forename: Christine M
authors.initials: CM
authors.lastname: Nunn
authors.forename: Cherelyn
authors.initials: C
authors.lastname: Vella
authors.forename: S W Annie
authors.initials: SW
authors.lastname: Bligh
mesh_terms: D004305:Dose-Response Relationship, Drug; D016333:HIV Protease / Q000378:metabolism*; D017320:HIV Protease Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D017320:HIV Protease Inhibitors; D054852:Small Molecule Libraries; D016333:HIV Protease