title: High-purity prostate circulating tumor cell isolation by a polymer nanofiber-embedded microchip for whole exome sequencing.
abstract: Handpick single cancer cells: a modified NanoVelcro Chip is coupled with ArcturusXT laser capture microdissection (LCM) technology to enable the detection and isolation of single circulating tumor cells (CTCs) from patients with prostate cancer (PC). This new approach paves the way for conducting next-generation sequencing (NGS) on single CTCs.
authors.affiliation: Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Science, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China; Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA.
authors.forename: Libo
authors.initials: L
authors.lastname: Zhao
authors.forename: Yi-Tsung
authors.initials: YT
authors.lastname: Lu
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Li
authors.forename: Kui
authors.initials: K
authors.lastname: Wu
authors.forename: Shuang
authors.initials: S
authors.lastname: Hou
authors.forename: Juehua
authors.initials: J
authors.lastname: Yu
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Shen
authors.forename: Dongxia
authors.initials: D
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.forename: Wei-Han
authors.initials: WH
authors.lastname: OuYang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Luo
authors.forename: Tom
authors.initials: T
authors.lastname: Lee
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
authors.forename: Chen
authors.initials: C
authors.lastname: Shao
authors.forename: Xun
authors.initials: X
authors.lastname: Xu
authors.forename: Mitch A
authors.initials: MA
authors.lastname: Garcia
authors.forename: Leland W K
authors.initials: LW
authors.lastname: Chung
authors.forename: Matthew
authors.initials: M
authors.lastname: Rettig
authors.forename: Hsian-Rong
authors.initials: HR
authors.lastname: Tseng
authors.forename: Edwin M
authors.initials: EM
authors.lastname: Posadas
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D059472:Exome / Q000235:genetics*; D023281:Genomics; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D046208:Microchip Analytical Procedures / Q000379:methods*; D057139:Nanofibers; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D017422:Sequence Analysis, DNA
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: circulating tumor cell; electrospun nanomaterials; microfluidics; prostate cancer; whole exome sequencing