title: Molecular cloning, characterization and expression analysis of ubiquitin protein ligase gene (As-ubpl) from Artemia sinica.
abstract: Ubiquitylation is an important protein post-translational regulation pathway, which is involved in controlling protein degradation, tumor occurrence and cell cycle regulation. E3 ubiquitin protein ligase (UBPL) plays a crucial role of the conjugation of activated ubiquitin to protein substrates and leads to targeting proteins for degradation by the proteasome. We amplified one full-length cDNA of the A. sinica UBPL (As-ubpl) gene by RACE technology. The full-length cDNA of As-ubpl is composed of 2931 bp, with a 2571 bp open reading frame (ORF) that encodes a polypeptide of 856 amino acids with a C2 domain, two domains with two conserved Trp (W) residues (WW) and a homologous to E6-AP Carboxyl Terminus (HECT) domain. The amount of As-ubpl showed from real-time PCR indicates that a high expression levels of As-ubpl at 20 h, 40 h and 3 days of embryo development, with highest expression levels appearing in the larval stage (40 h). Furthermore, As-ubpl transcripts were highly up-regulated under salinity (50‰) and low temperature stress (15 °C). These results indicate that As-ubpl is involved in protein regulation of the postdiapause development and in responses to salinity and low temperature stress.
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, PR China.
authors.forename: Zengrong
authors.initials: Z
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.forename: Yifei
authors.initials: Y
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Lv
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Wenting
authors.initials: W
authors.lastname: Xin
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D019295:Computational Biology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D044767:Ubiquitin-Protein Ligases