title: Revision of the biodistribution of uranyl in serum: is fetuin-A the major protein target?
abstract: Uranium is a natural actinide present as uranyl U(VI) species in aqueous environments. Its toxicity is considered to be chemical rather than radiotoxicological. Whatever the route of entry, uranyl reaches the blood, is partly eliminated via the kidneys, and accumulated in the bones. In serum, its speciation mainly involves carbonate and proteins. Direct identification of labile uranyl-protein complexes is extremely difficult because of the complexity of this matrix. Thus, until now the biodistribution of the metal in serum has not been described, and therefore, little is known about the metal transport mechanisms leading to bone accumulation. A rapid screening method based on a surface plasmon resonance (SPR) technique was used to determine the apparent affinities for U(VI) of the major serum proteins. A first biodistribution of uranyl was obtained by ranking the proteins according to the criteria of both their serum concentrations and affinities for this metal. Despite its moderate concentration in serum, fetuin-A (FETUA) was shown to exhibit an apparent affinity within the 30 nM range and to carry more than 80% of the metal. This protein involved in bone mineralization aroused interest in characterizing the U(VI) and FETUA interaction. Using complementary chromatographic and spectroscopic approaches, we demonstrated that the protein can bind 3 U(VI) at different binding sites exhibiting Kd from ∼30 nM to 10 μM. Some structural modifications and functional properties of FETUA upon uranyl complexation were also controlled. To our knowledge, this article presents the first identification of a uranyl carrier involved in bone metabolism along with the characterization of its metal binding sites.
authors.affiliation: CEA/DSV/iBEB/SBTN , Laboratoire d'Etude des Protéines Cibles, BP 17171 30 207 Bagnols sur Cèze Cédex, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Basset
authors.forename: Olivier
authors.initials: O
authors.lastname: Averseng
authors.forename: Pierre-Jean
authors.initials: PJ
authors.lastname: Ferron
authors.forename: Nicolas
authors.initials: N
authors.lastname: Richaud
authors.forename: Agnès
authors.initials: A
authors.lastname: Hagège
authors.forename: Olivier
authors.initials: O
authors.lastname: Pible
authors.forename: Claude
authors.initials: C
authors.lastname: Vidaud
mesh_terms: D000818:Animals; D001665:Binding Sites; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D020349:Surface Plasmon Resonance; D014501:Uranium / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*; D060749:alpha-2-HS-Glycoprotein / Q000737:chemistry / Q000378:metabolism*
chemical_list: D060749:alpha-2-HS-Glycoprotein; D014501:Uranium