title: Study of probiotic potential of four wild Lactobacillus rhamnosus strains.
abstract: The four wild Lactobacillus rhamnosus strains were examined in vitro for resistance to simulated gastro and intestinal juices, adhesion to HT-29 cells, antagonistic activity against enteric pathogens and immunomodulating activity. The strains L. rhamnosus SB5L, J5L and IN1L were able to survive in simulated gastro juice while the strain L. rhamnosus SB31L lost viability exposed to simulated gastro juice for 3 h. The four strains had high viability in simulated small intestinal juice with little loss (<1.0 cycle reduction). The strains SB5L, J5L and IN1L antagonized against Escherichia coli ATCC 25922, Salmonella enterica serovar Typhimurium ATCC 14028, Shigella sonnei ATCC 25931. The strain L. rhamnosus IN1L had the highest adhesive capability to HT-29 cells in vitro (251 bacteria cells per 100 HT-29 cells) compared to the other three L. rhamnosus strains. The live bacteria, cell wall and DNA of the four L. rhamnosus induced the secretion of pro-inflammatory cytokines IL-12 (p70), IFN-γ and TNF-α by human peripheral blood mononuclear cells (PBMCs). The levels of IL-12 (p70), IFN-γ and TNF-α produced by stimulated PBMCs were significantly higher (P < 0.05) than those of the control. Those data indicated that the four L. rhamnosus strains have the potential as the probiotic for human being use, although further studies are still needed.
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Technical Centre of Bright Dairy and Food Co. Ltd, Shanghai 200436, PR China. tyfjq@yahoo.com.cn
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Tuo
authors.forename: Weiqin
authors.initials: W
authors.lastname: Zhang
authors.forename: Lanwei
authors.initials: L
authors.lastname: Zhang
authors.forename: Lianzhong
authors.initials: L
authors.lastname: Ai
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.forename: Huaxi
authors.initials: H
authors.lastname: Yi
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion; D002473:Cell Wall; D002681:China; D016207:Cytokines / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000502:physiology; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D019073:HT29 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D056747:Immunomodulation; D007223:Infant; D007413:Intestinal Mucosa / Q000382:microbiology; D052201:Lacticaseibacillus rhamnosus / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D019936:Probiotics; D012486:Salmonella typhimurium / Q000502:physiology; D012764:Shigella sonnei / Q000502:physiology
chemical_list: D016207:Cytokines; D004269:DNA, Bacterial