title: Avian leukosis virus subgroup J associated with the outbreak of erythroblastosis in chickens in China.
abstract: BACKGROUND
  Emaciation, depression and lethargy were observed in two flocks of Chinese local breed and one flock of commercial layer chicken infected naturally from 2010 to 2011. The aims of this study were to diagnose.
  
  
  RESULTS
  Gross observation showed that severe enlargement of liver, spleen and kidney, and hemorrhage of thymus, muscle and glandular stomach in all submitted birds. The liver and lung of one flock had diffuse, multifocal white raised foci on the surface as well as on the cut-surface. Numerous erythrocytoblasts with bigger volume, basophilic cytoplasm and round nucleus were observed in blood and bone marrow smears. The same erythrocytoblasts were also found crowded in blood vessels and mesenchym of tissues by histological examination, and some had mitotic figures. PCR results showed that three flocks were positive for ALV-J with specific fragment of 924 bp, negative for AEV, ALV-A, ALV-B, Marek's disease virus (MDV) and Reticuloendotheliosis virus (REV). The results of immunohistochemistry showed that cytoplasm of histiocytes and erythrocytoblasts in lung and spleen sections was positive for ALV-J antigen.
  
  
  CONCLUSIONS
  These data demonstrated that erythroblastosis was all induced by ALV-J in the three different flocks. This is the first document report of erythroblastosis induced by ALV-J in China flocks.
authors.affiliation: Department of Fundamental Veterinary, Molecular pathology lab, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Yanping
authors.initials: Y
authors.lastname: Jiang
authors.forename: Linin
authors.initials: L
authors.lastname: Yu
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhao
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D001354:Avian Leukosis Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002645:Chickens; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D007668:Kidney / Q000473:pathology / Q000821:virology; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D008099:Liver / Q000473:pathology / Q000821:virology; D011201:Poultry Diseases / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D013154:Spleen / Q000473:pathology / Q000821:virology