title: Transcorneal electrical stimulation alters morphology and survival of retinal ganglion cells after optic nerve damage.
abstract: Traumatic optic nerve injury leads to retrograde death of retinal ganglion cells (RGCs), but transcorneal electrical stimulation (TES) can increase the cell survival rate. To understand the mechanisms and to further define the TES-induced effects we monitored in living animals RGC morphology and survival after optic nerve crush (ONC) in real time by using in vivo confocal neuroimaging (ICON) of the retina. ONC was performed in rats and ICON was performed before crush and on post-lesion days 3, 7 and 15 which allowed us to repeatedly record RGC number and size. TES or sham-stimulation were performed immediately after the crush and on post-injury day 11. Three days after ONC we detected a higher percentage of surviving RGCs in the TES group as compared to sham-treated controls. However, the difference was below significance level on day 7 and disappeared completely by day 15. The death rate was more variable amongst the TES-treated rats than in the control group. Morphological analysis revealed that average cell size changed significantly in the control group but not in stimulated animals and the morphological alterations of surviving neurons were smaller in TES-treated compared to control cells. In conclusion, TES delays post-traumatic cell death significantly. Moreover, we found "responder animals" which also benefited in the long-term from the treatment. Our in vivo cellular imaging results provide evidence that TES reduces ONC-associated neuronal swelling and shrinkage especially in RGCs which survived long-term. Further studies are now needed to determine the differences of responders vs. non-responders.
authors.affiliation: Otto-von-Guericke University Magdeburg, Institute of Medical Psychology, Leipziger Str. 44, 39120 Magdeburg, Germany. Petra.Henrich-Noack@med.ovgu.de
authors.forename: Petra
authors.initials: P
authors.lastname: Henrich-Noack
authors.forename: Nadine
authors.initials: N
authors.lastname: Voigt
authors.forename: Sylvia
authors.initials: S
authors.lastname: Prilloff
authors.forename: Anton
authors.initials: A
authors.lastname: Fedorov
authors.forename: Bernhard A
authors.initials: BA
authors.lastname: Sabel
mesh_terms: D000818:Animals; D002470:Cell Survival; D004558:Electric Stimulation; D008297:Male; D018613:Microscopy, Confocal; D009409:Nerve Crush; D059906:Neuroimaging; D020221:Optic Nerve Injuries / Q000473:pathology*; D051381:Rats; D012165:Retinal Ganglion Cells / Q000473:pathology*