title: Down regulation of TRAIL and FasL on NK cells by Cyclosporin A in renal transplantation patients.
abstract: TNF-related apoptosis-inducing ligand (TRAIL) and FasL can participate in cell mediated cytotoxicity via their death domain-mediated apoptotic signaling in the host-versus-graft disease occurred after renal transplantation. However, the effect of Cyclosporin A (CsA) commonly used as a drug to prevent and to treat renal transplant rejection, on these molecules have not been fully determined. In the present study, we found that with CsA administration, the expression of TRAIL and FasL predominantly on NK cells from renal transplantation patients was increased at day 5 after operation and went down to normal level on day 13. While, the levels of soluble TRAIL (sTRAIL) and sFasL in the serum increased within 25 days and went down to normal level three month later. In addition, we showed that a remarkable increase of TRAIL and FasL expression both on the surface of activated lymphocytes especially on NK cells and in the supernatants generated from mixed lymphocytes culture (MLC). Furthermore, the enhancement of these two molecules was greatly decreased by adding 500 ng/mL CsA at the beginning of MLC. We conclude that CsA may inhibit the transplant rejection partially by down-regulating the expression of TRAIL and FasL on NK cells.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Cheng
authors.forename: Zhu-Wei
authors.initials: ZW
authors.lastname: Xu
authors.forename: Zhou-Li
authors.initials: ZL
authors.lastname: Li
authors.forename: Chao-Jun
authors.initials: CJ
authors.lastname: Song
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Chen
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: An-Gang
authors.initials: AG
authors.lastname: Yang
authors.forename: Bo-Quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D016572:Cyclosporine / Q000008:administration & dosage*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006084:Graft Rejection / Q000209:etiology / Q000517:prevention & control*; D006801:Humans; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000517:prevention & control*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053222:Fas Ligand Protein; D053221:TNF-Related Apoptosis-Inducing Ligand; D016572:Cyclosporine