title: Crystallization and preliminary X-ray crystallographic studies of cPOP1.
abstract: Cellular pyrin domain-only protein 1 (cPOP1) is a pyrin domain (PYD)-containing protein that can regulate inflammation by preventing the assembly of inflammasome via direct interaction with ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain). In this study, cPOP1, corresponding to amino acids 1-87, was overexpressed in Escherichia coli using an engineered C-terminal polyhistidine tag. cPOP1 was then purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 3.6 Å from a crystal belonging to the cubic space group P2₁3 with unit-cell parameters a=b=c=94.12 Å, α=β=γ=90.00°.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Kyung Hoon
authors.initials: KH
authors.lastname: Do
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Park
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D006639:Histidine / Q000235:genetics; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary; D000071198:Pyrin; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D003598:Cytoskeletal Proteins; C000604192:MEFV protein, human; D010446:Peptide Fragments; D000071198:Pyrin; D011993:Recombinant Fusion Proteins; C033223:polyhistidine; D006639:Histidine
keywords: cPOP1; inflammasome; inflammation