title: Fabrication of a colorimetric biosensing platform for the detection of protein-DNA interaction.
abstract: Protein-DNA interaction plays important roles in many cellular processes, and there is an urgent demand for valid methods to monitor the interaction. In view of this, we propose a simple label-free colorimetric platform for the detection of protein-DNA interaction. Protein-DNA couples together with peroxidase-mimicking DNAzyme and exonuclease are elaborately incorporated into an integrated biosensing system. Besides the simplicity and efficiency, the strategy also has a great advantage for its universality in the detection of different protein-DNA couples. In our experiments, effective validation of our approach can be supported by two different protein-DNA couples (estrogen receptor α and nuclear factor kappa B). Experimental results show that the DNAzyme is competent to give rise to evident readout signals to monitor protein-DNA couples. Furthermore, with the substitution of DNA binding sequence in the probe, this method could be extended to a general platform for the detection of protein-DNA interaction.
authors.affiliation: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors.forename: Zonghuang
authors.initials: Z
authors.lastname: Ye
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.forename: Guifang
authors.initials: G
authors.lastname: Chen
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Shen
authors.forename: Nandi
authors.initials: N
authors.lastname: Zhou
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000378:metabolism; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D003124:Colorimetry / Q000191:economics / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D021881:DNA, Catalytic / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000378:metabolism; D054856:G-Quadruplexes; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D013451:Sulfonic Acids / Q000378:metabolism
chemical_list: D052160:Benzothiazoles; D021881:DNA, Catalytic; D047628:Estrogen Receptor alpha; D016328:NF-kappa B; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D004247:DNA; D009195:Peroxidase; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III