title: Droplets as reaction compartments for protein nanotechnology.
abstract: Extreme miniaturization of biological and chemical reactions in pico- to nanoliter microdroplets is emerging as an experimental paradigm that enables more experiments to be carried out with much lower sample consumption, paving the way for high-throughput experiments. This review provides the protein scientist with an experimental framework for (a) formation of polydisperse droplets by emulsification or, alternatively, of monodisperse droplets using microfluidic devices; (b) construction of experimental rigs and microfluidic chips for this purpose; and (c) handling and analysis of droplets.
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors.forename: Sean R A
authors.initials: SR
authors.lastname: Devenish
authors.forename: Miriam
authors.initials: M
authors.lastname: Kaltenbach
authors.forename: Martin
authors.initials: M
authors.lastname: Fischlechner
authors.forename: Florian
authors.initials: F
authors.lastname: Hollfelder
mesh_terms: D019020:Directed Molecular Evolution; D004655:Emulsions; D006601:Hexoses / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D008899:Mineral Oil / Q000737:chemistry; D036103:Nanotechnology; D010316:Particle Size; D016012:Poisson Distribution; D011136:Polysorbates / Q000737:chemistry; D014176:Protein Biosynthesis; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D004655:Emulsions; D006601:Hexoses; D011136:Polysorbates; D011506:Proteins; D011994:Recombinant Proteins; D013501:Surface-Active Agents; C018665:sorbitan monooleate; D008899:Mineral Oil