title: Effects of open versus laparoscopic nephrectomy techniques on oxidative stress markers in patients with renal cell carcinoma.
abstract: The aim of the study was to determine the concentration of lipid peroxidation products, the activity of selected antioxidant and lysosomal enzymes, and protease inhibitor in patients with renal cell carcinoma who underwent radical nephrectomy. The studied group included 44 patients: 21 of them underwent open surgery, while 23 underwent laparoscopy. Blood samples were collected three times: before treatment and 12 hours and five days after nephrectomy. In blood of participants, the concentration of thiobarbituric acid reactive substances (TBARS), the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx), and the activity of acid phosphatase (AcP), arylsulfatase (ASA), cathepsin D (CTSD), and α 1-antitrypsin (AAT) were assayed. No statistically significant differences in investigated parameters were found between studied groups. Moreover, TBARS concentration and CAT, SOD, and GPx activity were not altered in the course of both types of surgery. Five days after both open and laparoscopic nephrectomy techniques, AAT activity was higher than its activity 12 hours after the procedure. The obtained results suggest that laparoscopy may be used for nephrectomy as effectively as open surgery without creating greater oxidative stress. Reduced period of convalescence at patients treated with laparoscopy may be due to less severe response of acute-phase proteins.
authors.affiliation: The Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland. celestyna@o2.pl
authors.forename: Celestyna
authors.initials: C
authors.lastname: Mila-Kierzenkowska
authors.forename: Alina
authors.initials: A
authors.lastname: Woźniak
authors.forename: Tomasz
authors.initials: T
authors.lastname: Drewa
authors.forename: Bartosz
authors.initials: B
authors.lastname: Woźniak
authors.forename: Michał
authors.initials: M
authors.lastname: Szpinda
authors.forename: Ewa
authors.initials: E
authors.lastname: Krzyżyńska-Malinowska
authors.forename: Paweł
authors.initials: P
authors.lastname: Rajewski
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000328:Adult; D000368:Aged; D001192:Arylsulfatases / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002374:Catalase / Q000097:blood; D002402:Cathepsin D / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D010535:Laparoscopy; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood; D000515:alpha 1-Antitrypsin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D000515:alpha 1-Antitrypsin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000135:Acid Phosphatase; D001192:Arylsulfatases; D002402:Cathepsin D