title: [Detection of processed animal protein: European experience and perspectives].
abstract: European Commission Regulation (EC) No. 152/2009 imposes optical microscopy as the reference method for official controls to detect traces of animal protein in animal feed. Since 1 July 2004, the one-solvent technique has been the only authorised variant of optical microscopy. Its detection limit is 0.1% of meat-and-bone meal. Other techniques--using molecular biology (polymerase chain reaction, immunology), microscopy or near-infrared imaging--have been developed in the past ten years to supplement the official method, which has certain limitations. This paper compares and discusses the different techniques, highlighting the strengths of each technique in order to propose a feasible control scheme to improve the sensitivity and specificity of the technique for the detection of processed animal protein in livestock feed.
authors.affiliation: Epidémiologie et analyse de risques appliquées aux sciences vétérinaires (UREAR-ULg), Département des maladies infectieuses et parasitaires, Faculté de Médecine vétérinaire, Université de Liège, Boulevard de Colonster 20, B42, B-4000 Liège, Belgique.
authors.forename: B M
authors.initials: BM
authors.lastname: Plouvier
authors.forename: V
authors.initials: V
authors.lastname: Baeten
authors.forename: J P
authors.initials: JP
authors.lastname: Maudoux
authors.forename: E
authors.initials: E
authors.lastname: Vanopdenbosch
authors.forename: D
authors.initials: D
authors.lastname: Berkvens
authors.forename: G
authors.initials: G
authors.lastname: Degand
authors.forename: C
authors.initials: C
authors.lastname: Saegerman
mesh_terms: D000821:Animal Feed / Q000032:analysis / Q000592:standards*; D000818:Animals; D002845:Chromatography / Q000662:veterinary; D004044:Dietary Proteins / Q000032:analysis*; D005060:Europe; D005511:Food Handling / Q000592:standards; D007158:Immunologic Techniques / Q000662:veterinary; D008853:Microscopy / Q000379:methods / Q000592:standards / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017096:Prion Diseases / Q000517:prevention & control*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000592:standards / Q000662:veterinary; D019265:Spectroscopy, Near-Infrared / Q000662:veterinary
chemical_list: D004044:Dietary Proteins