title: Fluorescent sensor for bacterial recognition.
abstract: Boronic acid-based fluorescent sensor is one of the non-enzymatic methods used for the recognition of saccharides. Since bacterial membrane has polysaccharides with diol groups, boronic acids probe could be applied for rapid bacterial recognition. Escherichia coli (XL-1 blue) were recognized by applying (3-(5-(dimethylamino) naphthalene-1-sulfonamide) phenyl) boronic acid (DNSBA) as a sensor and the fluorescence recorded by fluorometer micro-plate reader. Results showed that, fluorescence records of DNSBA increase in a dose dependent manner upon increasing the bacterial cell numbers. Moreover, the increase in the number of bacterial cells induces a shift in the spectra due to the formation of the anionic form of boronic acid complex. Therefore, DNSBA is an efficient sensor for monitoring bacterial cells.
authors.affiliation: National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt. rehabamin@niles.edu.eg
authors.forename: Rehab
authors.initials: R
authors.lastname: Amin
authors.forename: Souad A
authors.initials: SA
authors.lastname: Elfeky
mesh_terms: D001897:Boronic Acids / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology* / Q000302:isolation & purification*; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001897:Boronic Acids; D011135:Polysaccharides, Bacterial; C010686:benzeneboronic acid