title: Effects of a synthetic bioactive peptide on neurite growth and nerve growth factor release in chondroitin sulfate hydrogels.
abstract: Previous work has revealed robust dorsal root ganglia neurite growth in hydrogels of chondroitin sulfate. In the current work, it was determined whether addition of a synthetic bioactive peptide could augment neurite growth in these matrices via enhanced binding and sequestering of growth factors. Fluorescence recovery after photobleaching studies revealed that addition of peptide slowed nerve growth factor diffusivity in chondroitin sulfate gels, but not in control gels of hyaluronic acid. Furthermore, cultures of chick dorsal root ganglia in gels of hyaluronic acid or chondroitin sulfate revealed enhanced growth in chondroitin sulfate gels only upon addition of peptide. Taken together, these results suggest a synergistic nerve growth factor-binding activity between this peptide and chondroitin sulfate.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
authors.forename: Aaron W
authors.initials: AW
authors.lastname: Conovaloff
authors.forename: Brooke L
authors.initials: BL
authors.lastname: Beier
authors.forename: Pedro P
authors.initials: PP
authors.lastname: Irazoqui
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000737:chemistry*; D020285:Cryoelectron Microscopy; D036681:Fluorescence Recovery After Photobleaching; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D020932:Nerve Growth Factor / Q000008:administration & dosage* / Q000737:chemistry; D016501:Neurites / Q000187:drug effects*; D009991:Oscillometry; D010455:Peptides / Q000737:chemistry; D011312:Pressure; D011485:Protein Binding; D013997:Time Factors
chemical_list: D020100:Hydrogels; D010455:Peptides; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D020932:Nerve Growth Factor
keywords: chondroitin sulfate; dorsal root ganglion; fluorescence recovery after photobleaching; growth factor binding; hydrogel; nerve growth factor; nerve regeneration