title: Expression of protein tyrosine kinase 6 (PTK6) in nonsmall cell lung cancer and their clinical and prognostic significance.
abstract: OBJECTIVE
  The aim of the study was to validate the expression of protein tyrosine kinase 6 (PTK6) in nonsmall cell lung cancer (NSCLC), and to evaluate its clinicopathological and prognostic significance.
  
  
  METHODS
  We first conducted a meta-analysis on the mRNA profiling data sets of NSCLC in the Oncomine database. Then, one of the most significantly upregulated tyrosine kinase targets, PTK6, was further validated by immunohistochemistry in 104 primary NSCLC tumors. Furthermore the association between PTK6 expression, the clinical parameters, and overall survival was further analyzed.
  
  
  RESULTS
  Using the Oncomine database, we identified a list of tyrosine kinase genes related to NSCLC, among which PTK6 was the second most overexpressed gene (median rank = 915, P = 2.9 × 10(-5)). We further confirmed that NSCLC tumors had a higher expression level of PTK6 than normal pulmonary tissues. Moreover, high PTK6 expression correlated positively with shorter overall survival time, but not with other clinicopathological characteristics. In the multivariate Cox regression model, high PTK6 expression was demonstrated to be an independent prognostic factor for NSCLC patients.
  
  
  CONCLUSIONS
  Our results validated that PTK6 was found to be overexpressed in a proportion of NSCLC samples, and was associated with a poor prognosis, suggesting that this subgroup of NSCLC patients might benefit from PTK6 inhibitors in the future.
authors.affiliation: Department of Laboratory Diagnostic, the 89th Hospital, Weifang, Shandong, People's Republic of China;
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jianrong
authors.initials: J
authors.lastname: Zhang
authors.forename: Yulian
authors.initials: Y
authors.lastname: Xu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Li
authors.forename: Sunxiao
authors.initials: S
authors.lastname: Chen
authors.forename: Anmei
authors.initials: A
authors.lastname: Deng
keywords: PTK6; nonsmall cell lung cancer; prognosis; target; tyrosine kinase