title: PEG2000-DPSE-coated quercetin nanoparticles remarkably enhanced anticancer effects through induced programed cell death on C6 glioma cells.
abstract: In this study, PEGylated nanoparticles quercetin drug delivery vehicles were investigated as carriers for anticancer drugs induced programed cell death (PCD). PEG2000-DPSE-coated quercetin nanoparticles were prepared and tumor cell killing efficacy was studied on glioma C6 cells and assayed for cell survival, apoptosis, or necrosis. The levels of ROS production and mitochondrial membrane potential (ΔΨm) were determined. Western blot assayed p53, p-p53, cytochrome C, and caspase proteins expression were also studied. Results indicate that PEG2000-DPSE-QUE-NPS showed dose-dependent cytotoxicity to C6 glioma cells and enhanced ROS accumulation induced upregulation of p53 protein, which was accompanied with an increase in cytochrome c and caspase-3 protein levels. These results support the hypothesis that quercetin nanoparticles-coated PEG2000-DPSE remarkably enhanced anticancer effect of induced programed cell death on C6 glioma cells. Overall, PEG2000-DPSE-coated quercetin nanoparticles showed promising potential as a drug carrier for cancer therapy.
authors.affiliation: Department of Hospital Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: JunJie
authors.initials: J
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: XuanLi
authors.initials: X
authors.lastname: Chen
authors.forename: LiPing
authors.initials: L
authors.lastname: Zhang
authors.forename: ShanQing
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005910:Glioma / Q000188:drug therapy / Q000473:pathology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009336:Necrosis; D011092:Polyethylene Glycols / Q000494:pharmacology* / Q000633:toxicity; D011794:Quercetin / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D020099:Coated Materials, Biocompatible; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D011092:Polyethylene Glycols; D011794:Quercetin; D007770:L-Lactate Dehydrogenase
keywords: PEG2000-DPSE; apoptosis; mitochondrial pathway; nanoparticles; necrosis; quercetin