title: Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats.
abstract: We evaluate the effects of low-level laser therapy (LLLT) on the histological modifications and temporal osteogenic genes expression during the initial phase of bone healing in a model of bone defect in rats. Sixty-four Wistar rats were divided into control and treated groups. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation (Ga-Al-As laser 830 nm, 30 mW, 0.028  cm², 1.071  W/cm², 1 min and 34 s, 2.8 Joules, 100  J/cm²) was performed for 1, 2, 3, and 5 sessions. Histopathology revealed that treated animals presented higher inflammatory cells recruitment, especially 12 and 36 h postsurgery. Also, a better tissue organization at the site of the injury, with the presence of granulation tissue and new bone formation was observed on days three and five postsurgery in the treated animals. The quantitative real time polymerase chain reaction showed that LLLT produced a significantly increase in mRNA expression of Runx-2, 12 h and three days post-surgery, a significant upregulation of alkaline phosphatase mRNA expression after 36 h and three days post-surgery and a significant increase of osteocalcin mRNA expression after three and five days. We concluded that LLLT modulated the inflammatory process and accelerated bone repair, and this advanced repair pattern in the laser-treated groups may be related to the higher mRNA expression of genes presented by these animals.
authors.affiliation: Department of Bioscience, Federal University of São Paulo, Av. Ana Costa, 95, Vila Mathias, Santos, Sao Paulo 11050-240, Brazil.
authors.forename: Kelly Rossetti
authors.initials: KR
authors.lastname: Fernandes
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Natália Camargo
authors.initials: NC
authors.lastname: Rodrigues
authors.forename: Carla
authors.initials: C
authors.lastname: Tim
authors.forename: Anderson Amaro
authors.initials: AA
authors.lastname: Santos
authors.forename: Nivaldo Antônio
authors.initials: NA
authors.lastname: Parizotto
authors.forename: Heloisa Selistre
authors.initials: HS
authors.lastname: de Araujo
authors.forename: Patrícia
authors.initials: P
authors.lastname: Driusso
authors.forename: Ana Claudia Muniz
authors.initials: AC
authors.lastname: Rennó
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000293:injuries / Q000528:radiation effects
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger; C493717:Runx2 protein, rat; D015675:Osteocalcin; D000469:Alkaline Phosphatase