title: S-adenosyl homocysteine hydrolase (SAHH) accelerates flagellar regeneration in Dunaliella salina.
abstract: S-adenosylhomocysteine hydrolase (SAHH) is an enzyme, which catalyzes the hydrolysis of S-adenosylhomocysteine (SAH) which is formed after the donation of the methyl group of S-adenosylmethionine (SAM) to a methyl acceptor in methylation reaction. As a potent regulator of methylation, SAHH plays a critical role in methylation reaction in the cells. Here we cloned the SAHH gene from unicellular green alga Dunaliella salina (dsSAHH) and investigated its effects on flagellar regeneration of D. salina, and found that dsSAHH was upregulated both at the protein and the transcription levels during pH shock-triggered flagellar regeneration of D. salina. The flagellar regeneration was accelerated when dsSAHH was overexpressed, but it was inhibited by SAHH inhibitor 3-deazaadenosine (DZA). Moreover, a receptor for activated C kinase 1 from D. salina (dsRACK1), which was identified to interact with dsSAHH, was increased when dsSAHH was overexpressed in D. salina as shown by real-time PCR. The findings of this study suggest that dsSAHH may participate in the regulation of flagellar regeneration of D. salina.
authors.affiliation: Laboratory for Cell Biology, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, Henan, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Li
authors.forename: Liqiang
authors.initials: L
authors.lastname: Zhu
authors.forename: Yunmeng
authors.initials: Y
authors.lastname: Yan
authors.forename: Dandan
authors.initials: D
authors.lastname: Chai
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Lexun
authors.initials: L
authors.lastname: Xue
mesh_terms: D043383:Adenosylhomocysteinase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005407:Flagella / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D016823:Volvocida / Q000201:enzymology* / Q000235:genetics
chemical_list: D043383:Adenosylhomocysteinase