title: Gene expression profiling of human oocytes developed and matured in vivo or in vitro.
abstract: The quality of the human oocyte determines the success of fertilization and affects the consequent embryo development, pregnancy and birth; it therefore serves as a basis for human reproduction and fertility. The possibility to evaluate oocyte quality in the in vitro fertilization programme is very limited. The only criterion which is commonly used to evaluate oocyte quality is its morphology. There is a mass of oocytes in the in vitro fertilization programme which are not fertilized in spite of normal morphology. In the past, several attempts focused on oocyte gene expression profiling by different approaches. The results elucidated groups of genes related to the human oocyte. It was confirmed that some factors, such as oocyte in vitro maturation, are detectable at the molecular level of human oocytes and their polar bodies in terms of gene expression profile. Furthermore, the first genetic evaluations of oocyte-like cells developed in vitro from human stem cells of different origin were performed showing that these cells express some genes related to oocytes. All these findings provide some new knowledge and clearer insights into oocyte quality and oogenesis that might be introduced into clinical practice in the future.
authors.affiliation: Reproductive Unit, Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Slajmerjeva 3, 1000 Ljubljana, Slovenia. irma.virant@gmail.com
authors.forename: Irma
authors.initials: I
authors.lastname: Virant-Klun
authors.forename: Katja
authors.initials: K
authors.lastname: Knez
authors.forename: Tomaz
authors.initials: T
authors.lastname: Tomazevic
authors.forename: Thomas
authors.initials: T
authors.lastname: Skutella
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000378:metabolism; D018929:Cell Culture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008423:Maternal Age; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis; D059705:Polar Bodies; D020133:Reverse Transcriptase Polymerase Chain Reaction