title: Proteomic analysis of elite soybean Jidou17 and its parents using iTRAQ-based quantitative approaches.
abstract: BACKGROUND
  Derived from Hobbit as the female parent and Zao5241 as the male parent, the elite soybean cultivar Jidou17 is significantly higher yielding and shows enhanced qualities and stronger resistance to non-biological stress than its parents. The purpose of this study is to understand the difference in protein expression patterns between Jidou17 and its parental strains and to evaluate the parental contributions to its elite traits.
  
  
  RESULTS
  Leaves (14 days old) from Jidou17 and its parental cultivars were analysed for differential expressed proteins using an iTRAQ-based (isobaric tags for relative and absolute quantitation) method. A total of 1269 proteins was detected, with 141 and 181 proteins in Jidou17 differing from its female and male parent, respectively. Functional classification and an enrichment analysis based on biological functions, biological processes, and cellular components revealed that all the differential proteins fell into many functional categories but that the number of proteins varied greatly for the different categories, with enrichment in specific categories. A pathway analysis indicated that the differentiated proteins were mainly classified into the ribosome assembly pathway. Protein expression clustering results showed that the expression profiles between Jidou17 and its female parent Hobbit were more similar than those between Jidou17 and its male parent Zao5241 and between the two parental strains. Therefore, the female parent Hobbit contributed more to the Jidou17 genotype.
  
  
  CONCLUSIONS
  This study applied a proven technique to study proteomics in 14-day-old soybean leaves and explored the depth and breadth of soybean protein research. The results provide new data for further understanding the mechanisms of elite cultivar development.
authors.affiliation: National Soybean Improvement Center Shijiazhuang Sub-Center, North China Key Laboratory of Soybean Biology and Genetic Improvement, Ministry of Agriculture, Cereal & Oil Crop Institute, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050031, P,R, China. mengchenzhang@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.forename: Feng
authors.initials: F
authors.lastname: Gu
authors.forename: Duan
authors.initials: D
authors.lastname: Liu
authors.forename: Changcheng
authors.initials: C
authors.lastname: Yin
authors.forename: Shuangjin
authors.initials: S
authors.lastname: Zhao
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Jianan
authors.initials: J
authors.lastname: Zhang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yang
authors.forename: Xu
authors.initials: X
authors.lastname: Zhan
authors.forename: Mengchen
authors.initials: M
authors.lastname: Zhang