title: Photo-induced antitumor effect of 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide.
abstract: We have applied a fluorescent molecule 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC) for tumor targeting and treatment. In this study, we investigated the photo-induced antitumor effect of BMVC. In vitro cell line studies showed that BMVC significantly killed TC-1 tumor cells at light dose greater than 40 J/cm(2). The fluorescence of BMVC in the tumor peaked at 3 hours and then gradually decreased to reach the control level after 24 hours. In vivo tumor treatment studies showed BMVC plus light irradiation (iPDT) significantly inhibited the tumor growth. At day 24 after tumor implantation, tumor volume was measured to be 225 ± 79 mm(3), 2542 ± 181 mm(3), 1533 ± 766 mm(3), and 1317 ± 108 mm(3) in the iPDT, control, light-only, and BMVC-only groups, respectively. Immunohistochemistry studies showed the microvascular density was significantly lower in the iPDT group. Taken together, our results demonstrated that BMVC may be a potent tumor-specific photosensitizer (PS) for PDT.
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10002, Taiwan.
authors.forename: Ya-Shuan
authors.initials: YS
authors.lastname: Chou
authors.forename: Cheng-Chung
authors.initials: CC
authors.lastname: Chang
authors.forename: Ta-Chau
authors.initials: TC
authors.lastname: Chang
authors.forename: Tsung-Lin
authors.initials: TL
authors.lastname: Yang
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
authors.forename: Pei-Jen
authors.initials: PJ
authors.lastname: Lou
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002227:Carbazoles / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005453:Fluorescence; D006801:Humans; D008027:Light; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000187:drug effects; D009369:Neoplasms / Q000188:drug therapy*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*
chemical_list: C486012:3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide; D000970:Antineoplastic Agents; D002227:Carbazoles; D017319:Photosensitizing Agents; D011726:Pyridinium Compounds