title: Marinococcus tarijensis sp. nov., a moderately halophilic bacterium isolated from a salt mine.
abstract: A Gram-stain-positive, coccoid-shaped, halophilic bacterium, strain SR-1(T), was isolated from a salt crystal obtained from a mine located in Tarija, Bolivia. The strain was investigated using a polyphasic approach. The optimum conditions for growth of strain SR-1(T) were reached at 5% (w/v) NaCl, pH 7.6 and 37-40 °C. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone was MK-7. The major cellular fatty acids of strain SR-1(T) were anteiso-C(15:0), anteiso-C(17:0) and iso-C(16:0). The DNA G+C content of strain SR-1(T) was 48.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed a close relationship between strain SR-1(T) and Marinococcus halophilus JCM 2479(T) (99.7% 16S rRNA gene sequence similarity), Marinococcus halotolerans KCTC 19045(T) (99.4%) and Marinococcus luteus KCTC 13214(T) (99.8%). However, strain SR-1(T) also showed low levels of DNA-DNA relatedness with these reference strains (47, 61 and 58%, respectively). On the basis of phenotypic differences and DNA-DNA hybridization results, strain SR-1(T) is considered to represent a novel species of the genus Marinococcus, for which the name Marinococcus tarijensis sp. nov. is proposed. The type strain is SR-1(T) ( =LMG 26930(T) =CECT 8130(T)).
authors.affiliation: Center of Biotechnology, Faculty of Sciences and Technology, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Balderrama-Subieta
authors.affiliation: Center of Biotechnology, Faculty of Sciences and Technology, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Guzmán
authors.affiliation: Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Minegishi
authors.affiliation: Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Akinobu
authors.initials: A
authors.lastname: Echigo
authors.affiliation: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Shimane
authors.affiliation: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hatada
authors.affiliation: Center of Biotechnology, Faculty of Sciences and Technology, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: Jorge
authors.initials: J
authors.lastname: Quillaguamán
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001838:Bolivia; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008906:Mining; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; C062629:menaquinone 7