title: Protoilludane sesquiterpenes from the wood decomposing fungus Granulobasidium vellereum (Ellis & Cragin) Jülich.
abstract: The secondary metabolites of the saprotrophic wood-decay basidiomycete fungus Granulobasidium vellereum were studied. Six sesquiterpenes were obtained; 2-hydroxycoprinolone (1), 8-deoxy-4a-hydroxytsugicoline (2), 8-deoxydihydrotsugicoline (3), which were previously not described, radulone A and B, and coprinolone ketodiol. Additionally, base-treatment of 1 yielded the diagnostic degradation products 1a and 1b, whereas radulone A was found to form 4 under mild acidic conditions. The structures were determined by NMR, MS, CD and polarimetry, along with biosynthetic considerations. Radulone A fully inhibited the spore germination of the potentially competing fungi Phlebiopsis gigantea, Coniophora puteana and Heterobasidion occidentale at 10μM, 500μM and 100μM, respectively. None of the other substances tested gave rise to any growth inhibition.
authors.affiliation: Department of Chemistry, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-75007 Uppsala, Sweden.
authors.forename: Christina L
authors.initials: CL
authors.lastname: Nord
authors.forename: Audrius
authors.initials: A
authors.lastname: Menkis
authors.forename: Rimvydas
authors.initials: R
authors.lastname: Vasaitis
authors.forename: Anders
authors.initials: A
authors.lastname: Broberg
mesh_terms: D005658:Fungi / Q000737:chemistry*; D008968:Molecular Conformation; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C506221:protoilludane