title: The effect of temperature of eye irrigation solution to reduce corneal endothelial cell loss during phacoemulsification: an in vitro model study.
abstract: OBJECTIVE
  To compare the efficiency of eye irrigation solution (balanced salt solution: BSS) stored in a refrigerator and air-conditioned room to reduce the number of corneal endothelial cell loss during the process of phacoemulsification.
  
  
  METHODS
  Porcine corneal endothelial cells (PCEC) isolated from porcine eyes were used as a model. The porcine eyes were obtained from a local slaughterhouse within 6 h after death and PCEC were cultured as standard method. The percentage of cell survival was evaluated by MTT assay.
  
  
  RESULTS
  The PCEC was successfully grown in the standard culturing system. The growth curve of PCEC demonstrated that log phase was reached in 3-6 days. The evaluation of heat effect (40-60 degrees C) to PCEC survival was shown that cells death was found at 55 and 60 degrees C which the percentage of cell survival reduced to 30% at 60 degrees C, 180 sec. Further observation on the efficiency of BSS stored in different conditions, refrigeration (8 degrees C) and air-conditioned room (25 degrees C) to PCEC survival was revealed that the number of cell survival increased from 40% to almost 70% when using refrigerated BSS as irrigation solution, while from air-conditioned BSS only at 55%.
  
  
  CONCLUSIONS
  From using PCEC as a model and mimic the process of irrigation in phacoemulsification, it was found that the eye irrigation solution (balanced salt solution: BSS) stored in the refrigerator had a better result in helping protection the corneal endothelial cell loss from heat damaged than BSS stored in air-conditioned room.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand. wanlaya@swu.ac.th
authors.forename: Wanlaya
authors.initials: W
authors.lastname: Uthaisang-Tanechpongtamb
authors.forename: Wiroj
authors.initials: W
authors.lastname: Limtrakarn
authors.forename: Somporn
authors.initials: S
authors.lastname: Reepolmaha
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016923:Cell Death; D002470:Cell Survival; D002478:Cells, Cultured; D055954:Corneal Endothelial Cell Loss / Q000517:prevention & control*; D004728:Endothelium, Corneal / Q000187:drug effects*; D066298:In Vitro Techniques; D009883:Ophthalmic Solutions / Q000494:pharmacology*; D018918:Phacoemulsification; D013552:Swine; D013696:Temperature; D007507:Therapeutic Irrigation
chemical_list: D009883:Ophthalmic Solutions