title: Designing a new chitinase with more chitin binding and antifungal activity.
abstract: Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. Chitinase Chit42 from Trichoderma atroviride PTCC5220 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin binding domain (ChBD). We have produced a chimeric chitinase with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Serratia marcescens Chitinase B. The fusion of ChBD improved the affinity to crystalline and colloidal chitin and also the enzyme activity of the chimeric chitinase when compared with the native Chit42. The chimeric chitinase showed higher antifungal activity toward phytopathogenic fungi.
authors.affiliation: National Institute of Genetic Engineering and Biotechnology, P.O. Box 149651/161, Tehran, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Matroodi
authors.forename: Mostafa
authors.initials: M
authors.lastname: Motallebi
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Zamani
authors.forename: Mehdi
authors.initials: M
authors.lastname: Moradyar
mesh_terms: D000528:Alternaria / Q000187:drug effects / Q000254:growth & development; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D012232:Rhizoctonia / Q000187:drug effects / Q000254:growth & development; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D002686:Chitin; D002688:Chitinases