title: Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes.
abstract: A novel bacteriophage, JX01, specifically infecting bovine Streptococcus agalactiae was isolated from milk of mastitis-affected cattle. The phage morphology showed that JX01 belongs to the family Siphoviridae, and this phage demonstrated a broad host range. Microbiological characterization demonstrated that nearly 90 % of JX01 phage particles were adsorbed after 2.5 min of incubation, that the burst size was 20 virions released per infected host cell, and that there was a latent period of 30 min. JX01 was thermal sensitive and showed acid and alkaline resistance (pH 3-11). The genome of JX01 was found to consist of a linear, double-stranded 43,028-bp DNA molecule with a GC content of 36.81 % and 70 putative open reading frames (ORFs) plus one tRNA. Comparative genome analysis revealed high similarity between JX01 and the prophage 315.2 of Streptococcus pyogenes.
authors.affiliation: Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Bai
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Yang
authors.forename: Fang
authors.initials: F
authors.lastname: Tang
authors.forename: Xuanhoa
authors.initials: X
authors.lastname: Nguyen
authors.forename: Guangjin
authors.initials: G
authors.lastname: Liu
authors.forename: Chengping
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D001482:Base Composition; D002417:Cattle; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017906:Siphoviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D017108:Streptococcus Phages / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D013292:Streptococcus agalactiae / Q000302:isolation & purification / Q000821:virology*; D053585:Virus Attachment
chemical_list: D004279:DNA, Viral