title: G protein beta3 subunit gene C825T and angiotensin converting enzyme gene insertion/deletion polymorphisms in hypertensive Tunisian population.
abstract: BACKGROUND
  We investigated the interaction between the G protein beta3 subunit (GNB3) C825T variant and angiotensin converting enzyme (ACE) Insertion/Deletion (I/D) polymorphism in hypertensive Tunisian population.
  
  
  METHODS
  Analyses of ACE and GNB3 genotypes were performed in 388 hypertensive patients and 425 healthy controls by polymerase chain reaction-restriction fragment length polymorphism. The plasma ACE activity was determined by a spectrophotometric method.
  
  
  RESULTS
  The ACE genotype distribution and allele frequencies were not significantly different between the hypertensive and normotensive subjects (p > 0.05). This polymorphism was not associated with hypertension (HTA) (OR = 0.93, 95% CI = 0.75 - 1.15; p = 0.50). In cases, subjects carrying the DD genotype exhibited higher plasma ACE activity than those with ID and II genotypes (p = 0.001). In this group, a linear regression analysis revealed that the ACE I/D polymorphism is independently associated with plasma ACE activity (p = 0.017). The genotypic distribution and allelic frequencies of the GNB3 C825T polymorphism were not significantly different between the two groups. This polymorphism was found to have no effect on the risk of HTA (OR = 1.14, 95% CI = 0.93 - 1.39; p = 0.21). We did not observe a significant interaction between the GNB3 gene and the ACE gene with HTA.
  
  
  CONCLUSIONS
  In this study, the I/D polymorphism is a significant independent predictor for variability of plasma ACE activity but the ACE I/D and GNB3 C825T polymorphisms are not significant factors for HTA in the Tunisian population. Moreover, we found no interaction between ACE D allele and GNB3 825T allele solely or combined with respect to HTA in the Tunisian population.
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta University Hospital, 1007 Tunis, Jebbari, Tunisia.
authors.forename: Ilhem Ayadi
authors.initials: IA
authors.lastname: Kabadou
authors.forename: Hayet
authors.initials: H
authors.lastname: Soualmia
authors.forename: Riadh
authors.initials: R
authors.lastname: Jemaa
authors.forename: Moncef
authors.initials: M
authors.lastname: Feki
authors.forename: Amani
authors.initials: A
authors.lastname: Kallel
authors.forename: Omar
authors.initials: O
authors.lastname: Souheil
authors.forename: Samah Haj
authors.initials: SH
authors.lastname: Taieb
authors.forename: Haifa
authors.initials: H
authors.lastname: Sanhaji
authors.forename: Naziha
authors.initials: N
authors.lastname: Kaabachi
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D017353:Gene Deletion; D005787:Gene Frequency; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D014416:Tunisia
chemical_list: C454420:G-protein beta3 subunit; D007703:Peptidyl-Dipeptidase A; D020962:Heterotrimeric GTP-Binding Proteins