title: Chemerin is an antimicrobial agent in human epidermis.
abstract: Chemerin, a chemoattractant ligand for chemokine-like receptor 1 (CMKLR1) is predicted to share similar tertiary structure with antibacterial cathelicidins. Recombinant chemerin has antimicrobial activity. Here we show that endogenous chemerin is abundant in human epidermis, and that inhibition of bacteria growth by exudates from organ cultures of primary human skin keratinocytes is largely chemerin-dependent. Using a panel of overlapping chemerin-derived synthetic peptides, we demonstrate that the antibacterial activity of chemerin is primarily mediated by Val(66)-Pro(85), which causes direct bacterial lysis. Therefore, chemerin is an antimicrobial agent in human skin.
authors.affiliation: Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Banas
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zabieglo
authors.forename: Gopinath
authors.initials: G
authors.lastname: Kasetty
authors.forename: Monika
authors.initials: M
authors.lastname: Kapinska-Mrowiecka
authors.forename: Julia
authors.initials: J
authors.lastname: Borowczyk
authors.forename: Justyna
authors.initials: J
authors.lastname: Drukala
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Murzyn
authors.forename: Brian A
authors.initials: BA
authors.lastname: Zabel
authors.forename: Eugene C
authors.initials: EC
authors.lastname: Butcher
authors.forename: Jens M
authors.initials: JM
authors.lastname: Schroeder
authors.forename: Artur
authors.initials: A
authors.lastname: Schmidtchen
authors.forename: Joanna
authors.initials: J
authors.lastname: Cichy
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D018925:Chemokines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004817:Epidermis / Q000276:immunology* / Q000382:microbiology*; D004926:Escherichia coli / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D015603:Keratinocytes / Q000276:immunology / Q000382:microbiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D023181:Antimicrobial Cationic Peptides; D018925:Chemokines; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; C478630:RARRES2 protein, human