title: Functional characterization of two alternatively spliced transcripts of tomato ABSCISIC ACID INSENSITIVE3 (ABI3) gene.
abstract: Alternative splicing can produce transcripts that encode proteins with altered functions. The transcripts of the ABSCISIC ACID INSENSITIVE3 (ABI3)/VIVIPAROUS1 (VP1) gene, which is an important component in abscisic acid (ABA) signaling, are subjected to alternative splicing in both monocotyledons and dicotyledons. We identified two alternatively spliced tomato (Solanum lycopersicum) SlABI3 transcripts, SlABI3-F and SlABI3-T, which encode the nucleus-localized full-length and truncated proteins, respectively. The tissue-specific accumulation of SlABI3-F and SlABI3-T was determined, particularly in seeds at different developmental stages and in response to phytohormonal and abiotic stress. Ectopic over-expression of SlABI3-F and SlABI3-T resulted in the induction of seed-specific genes SlSOM, SlEM1 and SlEM6 in vegetative tissues. However, over-expression of SlABI3-F, but not SlABI3-T, activated expression of the downstream gene SlABI5 and conferred hypersensitivity to exogenous ABA during seed germination and primary root growth. In addition, the SlABI3-F protein interacted with SlABI5 much stronger than SlABI3-T did in the yeast two-hybrid assay. These results suggest that SlABI3-F and SlABI3-T have similar and distinct functionality in the ABA signaling, dependent on which tissue/organ they accumulate in.
authors.affiliation: Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China. Gaoyongfeng0263@gmail.com
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Gao
authors.forename: Jikai
authors.initials: J
authors.lastname: Liu
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Sun
authors.forename: Ni
authors.initials: N
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.forename: Xiangli
authors.initials: X
authors.lastname: Niu
authors.forename: Fangming
authors.initials: F
authors.lastname: Xiao
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017398:Alternative Splicing / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D018525:Germination / Q000187:drug effects / Q000235:genetics; D005966:Glucuronidase / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics*; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D000040:Abscisic Acid; D005966:Glucuronidase