title: Organization of the extracellular portion of the macrophage galactose receptor: a trimeric cluster of simple binding sites for N-acetylgalactosamine.
abstract: The properties of the human macrophage galactose receptor have been investigated. Specificity for N-acetylgalactosamine (GalNAc) residues with exposed 3- and 4-hydroxyl groups explains virtually all of the results obtained from a recently expanded array of synthetic glycans and is consistent with a model for the structure of the binding site. This simple interaction is sufficient to explain the ability of the receptor to bind to tumor-cell glycans bearing Tn and sialyl-Tn antigens, but not to more elaborate O-linked glycans that predominate on normal cells. This specificity also allows for binding of parasite glycans and screening of an array of bacterial outer membrane oligosaccharides confirms that the receptor binds to a subset of these structures with appropriately exposed GalNAc residues. A key feature of the receptor is the clustering of binding sites in the extracellular portion of the protein, which retains the trimeric structure observed in the cell membrane. Chemical crosslinking, gel filtration, circular dichroism analysis and differential scanning calorimetry demonstrate that this trimeric structure of the receptor is stabilized by an α-helical coiled coil that extends from the surface of the membrane to the globular carbohydrate-recognition domains. The helical neck domains form independent trimerization domains. Taken together, these results indicate that the macrophage galactose receptor shares many of the features of serum mannose-binding protein, in which clusters of monosaccharide-binding sites serve as detectors for a simple epitope that is not common on endogenous cell surface glycans but that is abundant on the surfaces of tumor cells and certain pathogens.
authors.affiliation: Department of Life Sciences, Imperial College, Sir Ernst Chain Building, London SW7 2AZ, UK.
authors.forename: Sabine A F
authors.initials: SA
authors.lastname: Jégouzo
authors.forename: Adrián
authors.initials: A
authors.lastname: Quintero-Martínez
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Ouyang
authors.forename: Ália
authors.initials: Á
authors.lastname: dos Santos
authors.forename: Maureen E
authors.initials: ME
authors.lastname: Taylor
authors.forename: Kurt
authors.initials: K
authors.lastname: Drickamer
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry / Q000378:metabolism; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry* / Q000378:metabolism*; D008264:Macrophages / Q000737:chemistry; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D015295:Antigens, Tumor-Associated, Carbohydrate; D037181:Lectins, C-Type; C506225:MGL lectin, human; C040286:Tn antigen; D019158:N-Acetylneuraminic Acid; D000117:Acetylglucosamine
keywords: Carbohydrate-recognition domain; Galactose receptor; Glycan array; Glycan-binding receptor; Lectin