title: Bioeffects of a combination of trace elements on adipocyte biology.
abstract: The white adipose tissue plays a major role in the development of obesity and associated metabolic complications by producing a variety of pro and anti-inflammatory adipokines. Recently, studies in humans or in animals have shown a beneficial effect of certain trace elements such as zinc on insulin resistance and adipokine secretion. The aim of our study was to test the effect of a zinc-nickel-cobalt solution (ZnNiCo) on adipocyte function and to identify potential health effects of this solution in the context of obesity and associated disorders. No impact of ZnNiCo on adipogenesis was observed in 3T3-L1 cells. Gene expression in murine and human adipocytes was examined in the presence of ZnNiCo using whole genome microarrays. This transcriptomic analysis indicated that ZnNiCo affected the expression levels of genes in adipocytes under basal conditions or incubated with TNF-α and showed a down regulation of several inflammatory genes belonging to the cytokine and chemokine families (P < 0.01). These data were confirmed in mice fed with a high fat diet supplemented with ZnNiCo (P < 0.05). A modulation of NF-κB activation (evaluated by ELISA; P < 0.05) by ZnNiCo could explain at least in part these observations. The trace elements present in ZnNiCo are able to modulate the expression level of several inflammation related transcripts in adipocytes. These studies suggest that ZnNiCo could play a role in the prevention of inflammation in adipose tissue in obesity.
authors.affiliation: INRA, UMR_INRA 1260, Marseille, 13005, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Romier
authors.forename: Franck
authors.initials: F
authors.lastname: Tourniaire
authors.forename: Julie
authors.initials: J
authors.lastname: Marcotorchino
authors.forename: Erwan
authors.initials: E
authors.lastname: Gouranton
authors.forename: Julien
authors.initials: J
authors.lastname: Astier
authors.forename: Christiane
authors.initials: C
authors.lastname: Malezet
authors.forename: Eric
authors.initials: E
authors.lastname: Blouin
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Landrier
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000818:Animals; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D012996:Solutions; D014131:Trace Elements / Q000494:pharmacology*; D059467:Transcriptome / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D018925:Chemokines; D016328:NF-kappa B; D012996:Solutions; D014131:Trace Elements; D014409:Tumor Necrosis Factor-alpha