title: Determination of major components and fingerprint analysis of Flaveria bidentis (L.) Kuntze.
abstract: A sensitive high-performance liquid chromatography method coupled with photodiode array detection was developed for the simultaneous determination of six major constituents in Flaveria bidentis (L.) Kuntze: hyperoside, patuletin-3-O-glucoside, isorhamnetin 3-sulfate, astragalin, 6-methoxykaempferol-3-O-galactoside and α-terthienyl. The chemical fingerprint of Flaveria bidentis (L.) Kuntze leaves was established using raw materials of 12 batches in China. The chromatographic separations were obtained by using an Eclipse XDB-C18 reserved-phase column (150 × 4.6 mm i.d., 5 µm) using gradient elution with water (0.0125% trifluoroacetic acid, v/v) and acetonitrile at a flow rate of 1.0 mL/min, an operating temperature of 30°C and a detection wavelength of 360 nm. The new method was validated by linearity, limits of detection and quantification, precision, reproducibility, stability and recovery, and was also successfully applied to the simultaneous determination of components in Flaveria bidentis (L.) Kuntze. The results indicate that this multi-component determination method in combination with chromatographic fingerprint analysis is suitable for the quantitative analysis and identification of Flaveria bidentis (L.) Kuntze.
authors.affiliation: 1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Xie
authors.forename: Liping
authors.initials: L
authors.lastname: Ding
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D037142:Flaveria / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D005419:Flavonoids; D010936:Plant Extracts