title: Automated liquid microjunction surface sampling-HPLC-MS/MS analysis of drugs and metabolites in whole-body thin tissue sections.
abstract: BACKGROUND
  The aim of this work was to develop a fully automated liquid extraction-based surface sampling system utilizing a commercially available autosampler coupled with HPLC-MS/MS detection.
  
  
  RESULTS
  Discrete spots selected for droplet-based sampling and automated sample queue generation, for both the autosampler and MS, were enabled by using in-house developed software. In addition, co-registration of spatially resolved sampling positions and HPLC-MS information to generate heat maps of compounds monitored for subsequent data analysis was also available in the software. The system was evaluated with whole-body thin tissue sections from propranolol-dosed rats.
  
  
  CONCLUSIONS
  The spatial distributions of both the drug and its hydroxypropranolol glucuronide metabolites were consistent with previous studies employing other liquid extraction-based surface sampling methodologies.
authors.affiliation: Organic & Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA. kerteszv@ornl.gov
authors.forename: Vilmos
authors.initials: V
authors.lastname: Kertesz
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Van Berkel
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D008867:Microtomy; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011433:Propranolol / Q000032:analysis* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations; D011433:Propranolol