title: Carbohydrate derivatives from the roots of Brassica rapa ssp. campestris and their effects on ROS production and glutamate-induced cell death in HT-22 cells.
abstract: Phytochemical investigation of the roots of Brassica rapa ssp. campestris led to the isolation of three new carbohydrate derivatives, namely sucrose 3,3',4'-triisovalerate (2), sucrose 6,3',4'-triisovalerate (3), and ethanone-1-C-β-d-glucopyranoside (3,7-anhydro-1-deoxy-d-glycero-d-gulo-2-octulose, 6), along with four known carbohydrate derivatives, 2,6,3',4'-tetraisovalerate (1), ethyl β-d-glucopyranoside (4), n-butyl β-d-fructofuranoside (5), and n-pentyl β-d-fructofuranoside (7), which were initially isolated from plants of the Brassica genus. Structures of the isolated compounds were established by spectroscopic analyses, including UV, IR, MS, and NMR. All of the isolated carbohydrate derivatives were evaluated to determine their effect on ROS production and glutamate-induced cell death in HT-22 cells. Compound 6 showed the most significant ROS reduction and a protective effect with IC50 values of 69.4 ± 3.8 μM and 4.96 ± 0.32 μM, respectively, which were equivalent to those of the positive control, Trolox.
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University, Yongin 446-701, South Korea.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.forename: Jin-Gyeong
authors.initials: JG
authors.lastname: Cho
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Song
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
authors.forename: Hae-Gon
authors.initials: HG
authors.lastname: Chung
authors.forename: Myung-Sook
authors.initials: MS
authors.lastname: Choi
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Ahn
authors.forename: Geum-Soog
authors.initials: GS
authors.lastname: Kim
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
mesh_terms: D000818:Animals; D029741:Brassica rapa / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry* / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D005947:Glucose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013395:Sucrose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology
chemical_list: D002241:Carbohydrates; D017382:Reactive Oxygen Species; C585055:ethanone-1-C-beta-D-glucopyranoside; D018698:Glutamic Acid; D013395:Sucrose; D005947:Glucose