title: Antitumor activity of bee pollen polysaccharides from Rosa rugosa.
abstract: In the present study, bee pollen polysaccharides from Rosa rugosa (WRPP) were extracted and fractionated. WRPP were purified to neutral (WRPP-N) and acidic polysaccharides (WRPP-1, WRPP-2) with DEAE-Cellulose. WRPP-N were mainly composed of glucose, mannose, arabinose and galactose, indicating the existence of glucan, arabinogalactan (AG) and mannoglucan. WRPP-1 mainly consisted of rhamnose (3.0%), galacturonic acid (12.4%), galactose (24.7%) and arabinose (53.9%), and contained a large proportion of AGs. WRPP-2 consisted of rhamnose (7.8%), galacturonic acid (23.0%), galactose (15%) and arabinose (48.7%), while WRPP-2 contained more galacturonic acid compared to WRPP-1. WRPP-1 and WRPP-2 were composed by type I rhamnogalacturonan (RG-I), homogalacturonan (HG) and AG fragments, while WRPP-2 contained more HG and RG-I. All the fractions had significant anti-proliferative activity in HT-29 and HCT116 cells; the neutral and acidic fractions were shown to have significant synergistic effects which accounted for the antitumor activity of bee pollen polysaccharides from Rosa rugosa in vitro.
authors.affiliation: School of Life Sciences, Northeast Normal University, Jilin 130024, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Diao
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Qipin
authors.initials: Q
authors.lastname: Gao
authors.forename: Yifa
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001516:Bees / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D004354:Drug Screening Assays, Antitumor; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008970:Molecular Weight; D011058:Pollen / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027826:Rosa / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides