title: Genetic variability of Echinococcus granulosus from the Tibetan plateau inferred by mitochondrial DNA sequences.
abstract: To analyse genetic variability and population structure, 84 isolates of Echinococcus granulosus (Cestoda: Taeniidae) collected from various host species at different sites of the Tibetan plateau in China were sequenced for the whole mitochondrial nad1 (894 bp) and atp6 (513 bp) genes. The vast majority were classified as G1 genotype (n=82), and two samples from human patients in Sichuan province were identified as G3 genotype. Based on the concatenated sequences of nad1+atp6, 28 different haplotypes (NA1-NA28) were identified. A parsimonious network of the concatenated sequence haplotypes showed star-like features in the overall population, with NA1 as the major haplotype in the population networks. By AMOVA it was shown that variation of E. granulosus within the overall population was the main pattern of the total genetic variability. Neutrality indexes of the concatenated sequence (nad1+atp6) were computed by Tajima's D and Fu's Fs tests and showed high negative values for E. granulosus, indicating significant deviations from neutrality. FST and Nm values suggested that the populations were not genetically differentiated.
authors.affiliation: Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yan
authors.forename: Hua-Ming
authors.initials: HM
authors.lastname: Nie
authors.forename: Zhong-Rong
authors.initials: ZR
authors.lastname: Jiang
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Yang
authors.forename: Shi-Jin
authors.initials: SJ
authors.lastname: Deng
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.forename: Yu-Bao
authors.initials: YB
authors.lastname: Yan
authors.forename: Dawa
authors.initials: D
authors.lastname: Tsering
authors.forename: Wei-Shu
authors.initials: WS
authors.lastname: Kong
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Jia-Hai
authors.initials: JH
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.forename: De-Ying
authors.initials: DY
authors.lastname: Yang
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Wang
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Gu
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Peng
authors.forename: Guang-You
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000531:Altitude; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D003710:Demography; D004443:Echinococcosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000235:genetics* / Q000502:physiology; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D018609:Tibet / Q000453:epidemiology
chemical_list: D004272:DNA, Mitochondrial
keywords: Echinococcus granulosus; Genetic variability; Mitochondrial DNA; Tibetan plateau