title: Osteoblastic potency of bone marrow cells cultivated on functionalized biometals with cyclic RGD-peptide.
abstract: The fixation of cementless endoprostheses requires excellent fixation at the bone implant interface. Although the surface structures of these implants are designed to promote osteoblastic differentiation, poor bone quality may prevent or delay osseointegration. There is evidence that RGD peptides known as recognition motifs for various integrins, promote cellular adhesion, influence cellular proliferation, and differentiation of local cells. In this study, five different metal surfaces were analyzed: Sandblasted (TiSa) and polished (TiPol) Ti6Al4V, porocoated (CCPor) and polished (CCPol) cobalt chrome and polished stainless steel (SS) were coated by ethanol amine and poly(ethylene glycol) to attach covalently RGD peptides. Human mesenchymal stromal cells of healthy donors were cultivated onto prior functionalized metal surfaces for 14 days without osteogenic stimulation. Cell proliferation and differentiation were quantitatively evaluated for native (I), NaOH pre-activated (II), NaOH pre-activated, and PEG-coated (III) as well as for RGD (IV) coated surfaces. The RGD immobilization efficiency was analyzed by epi-fluorescence spectroscopy, cell morphology was documented by light and scanning electron microscopy. The RGD-binding efficiency was TiSa > TiPol > SS > CCPor > CCPol. RGD coated surfaces showed the highest average cell proliferation on CCPol > SS > CCPor > TiSa ≥ TiPol, whereas cellular differentiation mostly correlated with the observed proliferation results, such as CCPol > TiSa > SS > CCPor > TiPol. Considering statistical analyses (significance level of α = 0.05), the RGD-coating of all biometals in comparison and in respect of their particular controls showed no significant improvement in cellular proliferation and osteoblastic differentiation.
authors.affiliation: Orthopaedic Department, University of Duisburg-Essen, Germany; Orthopaedic Department, Heinrich-Heine-University Medical School, Düsseldorf, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Jäger
authors.forename: C
authors.initials: C
authors.lastname: Böge
authors.forename: R
authors.initials: R
authors.lastname: Janissen
authors.forename: D
authors.initials: D
authors.lastname: Rohrbeck
authors.forename: T
authors.initials: T
authors.lastname: Hülsen
authors.forename: S
authors.initials: S
authors.lastname: Lensing-Höhn
authors.forename: R
authors.initials: R
authors.lastname: Krauspe
authors.forename: M
authors.initials: M
authors.lastname: Herten
mesh_terms: D000497:Alloys; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D002858:Chromium Alloys / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry / Q000494:pharmacology*; D013194:Staining and Labeling; D013193:Stainless Steel / Q000494:pharmacology; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology; D014131:Trace Elements / Q000494:pharmacology*
chemical_list: D000497:Alloys; D002858:Chromium Alloys; D020099:Coated Materials, Biocompatible; D010456:Peptides, Cyclic; D014131:Trace Elements; C507169:cyclic arginine-glycine-aspartic acid peptide; D013193:Stainless Steel; C031462:titanium alloy (TiAl6V4); D014025:Titanium
keywords: RGD peptide; bone marrow; endoprosthesis; mesenchymal stem cells; osteoblast