title: Maribacter aestuarii sp. nov., isolated from tidal flat sediment, and an emended description of the genus Maribacter.
abstract: A Gram-staining-negative, strictly aerobic bacterial strain, motile by gliding, designated GY20(T), was isolated from a tidal flat at Gwangyang Bay, South Korea. Cells were moderately halophilic, catalase- and oxidase-positive rods. Growth of strain GY20(T) was observed at 10-30 °C (optimum, 25 °C), at pH 6.5-11.0 (optimum, pH 7.0-8.0) and in the presence of 1-5% (w/v) NaCl (optimum, 2-3%). MK-6 was detected as the sole isoprenoid quinone, and iso-C(15:0), iso-C(15:1) G, summed feature 3 (comprising C(16:1)ω7c and/or C(16:1)ω6c), summed feature 9 (iso-C(17:1)ω9c and/or 10-methyl C(16:0)), iso-C(17:0) 3-OH and iso-C(15:0) 3-OH as major fatty acids. Strain GY20(T) contained phosphatidylethanolamine, one unidentified glycolipid and one unidentified lipid as major polar lipids. The G+C content of the genomic DNA was 39.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY20(T) formed a tight phyletic lineage with members of the genus Maribacter. Strain GY20(T) was most closely related to Maribacter dokdonensis DSW-8(T), with sequence similarity of 96.2%. On the basis of phenotypic, chemotaxonomic and molecular properties, strain GY20(T) was shown to represent a novel species within the genus Maribacter, for which the name Maribacter aestuarii sp. nov. is proposed. The type strain is GY20(T) ( =KACC 16440(T) =JCM 18631(T)). An emended description of the genus Maribacter is also proposed.
authors.affiliation: Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Naysim
authors.initials: N
authors.lastname: Lo
authors.affiliation: Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Hyun Mi
authors.initials: HM
authors.lastname: Jin
authors.affiliation: Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6