title: Secreted recombinant P53 protein from Pichia pastoris is a useful antigen for detection of serum p53: autoantibody in patients with advanced colorectal adenocarcinoma.
abstract: The detection of P53 alteration by serological method is easier to perform, does not require tumor tissues and is of interest for patients monitoring. In this study, we described the development of a home made ELISA test based on recombinant human P53 protein produced in Pichia pastoris and used as antigen for the detection of serum p53-Abs in colorectal carcinoma patients. The human P53 was secreted as a His-tagged protein by recombinant KM71 strain (Kα21) via the peptide signal α of the Saccharomyces cerevisiae mating type gene. The recombinant P53-His was able to detect p53-Abs in 23.4% of patients. Serum p53-Abs correlated significantly with surgical treatment (P = 0.007), relapse during follow-up (P = 0.036), depth of invasion (P = 0.036) and the level of CA19-9 (P = 0.034). Survival analysis showed that patients negative for serum p53-Abs exhibited a prolonged disease free survival period (P log rank = 0.012). In conclusion, the secreted recombinant human P53-His produced in P. pastoris seems to be a useful antigen for detection of serum p53 Abs in patients with colorectal carcinoma.
authors.affiliation: Laboratory of Biomass Valorisation and Production of Eukaryotic Proteins, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3038 Sfax, Tunisia.
authors.forename: Salma
authors.initials: S
authors.lastname: Abdelmoula-Souissi
authors.forename: Nourane
authors.initials: N
authors.lastname: Zouari
authors.forename: Imen
authors.initials: I
authors.lastname: Miladi-Abdenadher
authors.forename: Ouhoud
authors.initials: O
authors.lastname: Yaich-Kolsi
authors.forename: Ines
authors.initials: I
authors.lastname: Ayadi-Masmoudi
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Khabir
authors.forename: Hatem
authors.initials: H
authors.lastname: Masmoudi
authors.forename: Mounir
authors.initials: M
authors.lastname: Frikha
authors.forename: Raja
authors.initials: R
authors.lastname: Mokdad-Gargouri
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis* / Q000276:immunology* / Q000401:mortality; D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology* / Q000401:mortality; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000097:blood / Q000276:immunology / Q000302:isolation & purification; D016159:Tumor Suppressor Protein p53 / Q000097:blood / Q000235:genetics / Q000276:immunology*
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D011993:Recombinant Fusion Proteins; D016159:Tumor Suppressor Protein p53