title: Preparative separation of flavonoids in plant extract of Smilacis Glabrae Roxb. by high performance counter-current chromatography.
abstract: Four flavonoids, isoastilbin, astilbin, isoengelitin, and engelitin were isolated and purified simultaneously from Smilacis Glabrae Roxb. for the first time by high performance counter-current chromatography using a system consisting of n-hexane-n-butanol-water (1:2:3, v/v/v). A total of 392.6 mg of astilbin, 71.4 mg of isoastilbin, 47.4 mg of engelitin, and 10.3 mg of isoengelitin were purified from 1.89 g of the ethyl acetate extract of Smilacis Glabrae Roxb. in six runs, each at over 94.51% purity as determined by HPLC. The structures of the four compounds were identified by their retention time, the LC-ESI-MS(n) in the negative ion mode, and confirmed by (1)H-NMR experiments. The characteristic LC-ESI-MS fragmentation patterns of the four compounds were discussed.
authors.affiliation: The College of Chemistry, Changchun Normal University, China, Changchun.
authors.forename: Huirong
authors.initials: H
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zong
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D019668:Liliaceae / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids