title: Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.
abstract: A mecC (mecALGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.
authors.affiliation: Institute of Medical Microbiology, University Hospital Münster, Münster, Germany. kbecker@uni-muenster.de
authors.forename: Karsten
authors.initials: K
authors.lastname: Becker
authors.forename: Anders R
authors.initials: AR
authors.lastname: Larsen
authors.forename: Robert L
authors.initials: RL
authors.lastname: Skov
authors.forename: Gavin K
authors.initials: GK
authors.lastname: Paterson
authors.forename: Mark A
authors.initials: MA
authors.lastname: Holmes
authors.forename: Artur J
authors.initials: AJ
authors.lastname: Sabat
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Friedrich
authors.forename: Robin
authors.initials: R
authors.lastname: Köck
authors.forename: Georg
authors.initials: G
authors.lastname: Peters
authors.forename: André
authors.initials: A
authors.lastname: Kriegeskorte
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial