title: Establishment of a novel dsRed NOD/Scid mouse strain to investigate the host and tumor cell compartments.
abstract: Here we describe a NOD/Scid mouse strain expressing the dsRed transgene. The strain is maintained by inbreeding of homozygous dsRed NOD/Scid siblings, and expresses red fluorescence from various organs. The model allows engraftment of human tumor tissue, and engrafted tumors were separated into stromal and malignant cell compartments. Furthermore, we compared tumor-associated and normal fibroblast for expression of fibroblast-associated markers, and identified a marker panel that was upregulated in the tumor-associated fibroblasts. In conclusion, we propose that this model may be used in a variety of studies of tumor progression and to elucidate the role of the tumor microenvironment.
authors.affiliation: Oncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
authors.forename: Hege Karine
authors.initials: HK
authors.lastname: Jacobsen
authors.forename: Linda
authors.initials: L
authors.lastname: Sleire
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Inger Anne
authors.initials: IA
authors.lastname: Netland
authors.forename: Ercan
authors.initials: E
authors.lastname: Mutlu
authors.forename: Hilde
authors.initials: H
authors.lastname: Førde
authors.forename: Paal-Henning
authors.initials: PH
authors.lastname: Pedersen
authors.forename: Donald
authors.initials: D
authors.lastname: Gullberg
authors.forename: Per Øyvind
authors.initials: PØ
authors.lastname: Enger
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation / Q000379:methods; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism; D019076:Transgenes; D014183:Transplantation, Heterologous; D015854:Up-Regulation
chemical_list: D008164:Luminescent Proteins; C413663:fluorescent protein 583