title: Analysis of Total Urine Proteins: Towards A Non-Invasive Approach for Diagnosis of Visceral Leishmaniasis.
abstract: Diagnosis of Visceral Leishmaniasis (VL) is a major obstacle in the control of this disease, as demonstration of parasite by splenic or bone-marrow aspiration is almost impossible in the peripheral rural areas because of lack of facilities. In order to identify a non-invasive biomarker for VL, we employed two-dimensional electrophoresis (2D-PAGE) coupled with mass spectrometry through a comparison of urinary proteome. Though, we did not find the biomarker protein for VL, our findings provide some basic insights for future development of non invasive diagnostic tool.
authors.affiliation: Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Singh
authors.forename: Shyam
authors.initials: S
authors.lastname: Sundar
keywords: 2D-PAGE; MALDI-TOF; Visceral leishmaniasis; urinary proteins