title: [Change of T cell TCR-CD3 complex-mediated gene expression pattern in lead poisoning patients].
abstract: OBJECTIVE
  In order to study the feature of T cell TCR-CD3 complex-mediated gene in lead poisoning patients.
  
  
  METHODS
  Real-time PCR with SYBR Green I technique was used for determination of the expression levels of CD3 genes in peripheral blood mononuclear cells of 46 cases lead poisoning patients (11 cases in observation group and 35 cases in mild lead poisoning group) and 31 cases in control group.
  
  
  RESULTS
  The median expression levels of CD3γ gene in observation group and mild lead poisoning group (6.89%, 5.87 %) were higher than the control group (P < 0.05). The median expression levels of CD3δ gene in observation group and mild lead poisoning group (0.54%, 0.70%) were lower than the control group (P < 0.05). The median expression levels of CD3ε gene in observation group and mild lead poisoning group (10.22%, 6.08%) were higher than the control group (P < 0.05). A significant Positive correlation was found between CD3γ, CD3ε and seniority in lead poisoning patients. A significant negative correlation was found between CD3ε and blood ZPP, urea δ-ALA (r = -0.358, P < 0.05; r = -0.385, P < 0.05), but there was no significant correlation between them after controlling for blood lead, urea lead. The expression levels of CD3 genes prove to be a descending order of CD3γ, CD3ε, CD3δ in control group, while it was changed for CD3ε, CD3γ, CD3δ in the observation group as well as in mild lead poisoning group.
  
  
  CONCLUSIONS
  Expression of T cell TCR-CD3 complex-mediated gene was changed in lead poisoning patients, it might be related to the body immunodeficiency. The expression level of CD3ε gene can be used as sensitive immune function screening indicator in Lead poisoning patients.
authors.affiliation: Guangzhou Occupational Diseases Prevention and Treatment Center, Guangzhou, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.forename: Qiu-yue
authors.initials: QY
authors.lastname: Lin
authors.forename: Si-chu
authors.initials: SC
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Jing-dong
authors.initials: JD
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007855:Lead Poisoning / Q000276:immunology*; D008297:Male; D009784:Occupational Diseases / Q000276:immunology*; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017260:Receptor-CD3 Complex, Antigen, T-Cell