title: Effects of cell surface α2-3 sialic acid on osteogenesis.
abstract: A cell culture model of osteoblast differentiation was applied in our study of the effect of sialic acid on the osteogenesis by using the pre-osteoblast of MC3T3-E1 subclone 14 cells. Following the treatment of different concentrations of α2,3-neuraminidase, which specifically removed the α2-3 sialic acid from cell surface, a significant decrease of α2-3 sialic acid was detected with fluorescein isothiocyanate (FITC)-labeled Maackia amurensis lectin (MAL-II) by flow cytometry analysis. von Kossa staining showed that the bone mineralization decreased in MC3T3-E1 subclone 14 cells after the treatment of α2,3-neuraminidase for 2 weeks. However α2,3-neuraminidase did not affect the formation of osteoblasts in MC3T3-E1 subclone 14 cells, which was demonstrated by positive alkaline phosphatase (ALP)-staining. Characteristic biological markers and osteoblast-like cell-related factors of osteoblastic cells were also examined. Both RT-PCR and Western blot analysis demonstrated that the expression of bone sialoprotein (BSP), osteoprotegerin (OPG), and vitamin D receptor (VDR) were significantly decreased when α2-3 sialic acid expression decreased on the cell surface, while the expression of osteocalcin (OC) and osteopontin (OPN) remained unchanged. We propose a hypothesis that α2-3 sialic acid affects bone mineralization but not osteogenic differentiation.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, 215007, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Guiying
authors.initials: G
authors.lastname: Xu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhou
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Wei
authors.forename: Shiliang
authors.initials: S
authors.lastname: Wu
mesh_terms: D000115:Acetylesterase / Q000494:pharmacology; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002460:Cell Line; D005109:Extracellular Matrix / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D012794:Sialic Acids / Q000494:pharmacology*
chemical_list: D053244:Osteoprotegerin; D018167:Receptors, Calcitriol; D012794:Sialic Acids; D015675:Osteocalcin; D053495:Osteopontin; D000115:Acetylesterase; C046298:sialate O-acetylesterase