title: Methylated N-(4-N,N-dimethylaminocinnamyl) chitosan-coated electrospray OVA-loaded microparticles for oral vaccination.
abstract: The purpose of this study was to prepare microparticles entrapping ovalbumin (OVA) as a model antigen to induce immune responses in mice following oral vaccination. In this study, calcium-alginate and calcium-yam-alginate microparticles were prepared by crosslinking alginate with calcium chloride solution using an electrospraying technique. 0.1% (w/v) of methylated N-(4-N,N-dimethylaminocinnamyl) chitosan (TM65CM50CS) was used to coat microparticles entrapping an initial OVA of 20% w/w to polymer. The results indicated that the coated microparticles were spherical and had a smooth surface, with an average size of 1-3 μm, and were positively charged. In addition, the particles demonstrated a greater swelling and mucoadhesive properties than did uncoated microparticles. The in vitro release from the microparticles indicated that the coated microparticles resulted in more sustained release than uncoated microparticles. The cytotoxicity results showed that all of the formulations were safe. The in vivo oral administration demonstrated that at the same amount of 250 μg OVA, coated microparticles exhibited the highest in vivo adjuvant activity in both IgG and IgA immunogenicity.
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Tittaya
authors.initials: T
authors.lastname: Suksamran
authors.forename: Tanasait
authors.initials: T
authors.lastname: Ngawhirunpat
authors.forename: Theerasak
authors.initials: T
authors.lastname: Rojanarata
authors.forename: Warayuth
authors.initials: W
authors.lastname: Sajomsang
authors.forename: Tasana
authors.initials: T
authors.lastname: Pitaksuteepong
authors.forename: Praneet
authors.initials: P
authors.lastname: Opanasopit
mesh_terms: D000268:Adhesiveness; D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000737:chemistry*; D018938:Caco-2 Cells; D002470:Cell Survival; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000737:chemistry; D008745:Methylation; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000737:chemistry*; D013552:Swine; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D000464:Alginates; D000941:Antigens; D006603:Hexuronic Acids; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014612:Vaccines; D020723:Glucuronic Acid; D010047:Ovalbumin; D048271:Chitosan