title: Human platelet antigen genotyping and expression of CD109 (human platelet antigen 15) mRNA in various human cell types.
abstract: CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34- PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea.
authors.forename: Sang Mee
authors.initials: SM
authors.lastname: Hwang
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ho Eun
authors.initials: HE
authors.lastname: Chang
authors.forename: Yun Ji
authors.initials: YJ
authors.lastname: Hong
authors.forename: Taek Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
authors.forename: Kyoung Un
authors.initials: KU
authors.lastname: Park
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
authors.forename: Kyou-Sup
authors.initials: KS
authors.lastname: Han
mesh_terms: D015703:Antigens, CD / Q000235:genetics*; D016824:Antigens, Human Platelet / Q000235:genetics*; D001402:B-Lymphocytes / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005838:Genotype; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D016824:Antigens, Human Platelet; C120523:CD109 protein, human; D058851:GPI-Linked Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger