title: Chip-based nLC-TOF-MS is a highly stable technology for large-scale high-throughput analyses.
abstract: Many studies focused on the discovery of novel biomarkers for the diagnosis and treatment of disease states are facilitated by mass spectrometry-based technology. HPLC coupled to mass spectrometry is widely used; miniaturization of this technique using nano-liquid chromatography (LC)-mass spectrometry (MS) usually results in better sensitivity, but is associated with limited repeatability. The recent introduction of chip-based technology has significantly improved the stability of nano-LC-MS, but no substantial studies to verify this have been performed. To evaluate the temporal repeatability of chip-based nano-LC-MS analyses, N-glycans released from a serum sample were repeatedly analyzed using nLC-PGC-chip-TOF-MS on three non-consecutive days. With an average inter-day coefficient of variation of 4 %, determined on log10-transformed integrals, the repeatability of the system is very high. Overall, chip-based nano-LC-MS appears to be a highly stable technology, which is suitable for the profiling of large numbers of clinical samples for biomarker discovery.
authors.affiliation: Department of Chemistry, University of California Davis, Davis, CA 95616, USA. lruhaak@ucdavis.edu
authors.forename: L Renee
authors.initials: LR
authors.lastname: Ruhaak
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Taylor
authors.forename: Suzanne
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Karen
authors.initials: K
authors.lastname: Kelly
authors.forename: Gary S
authors.initials: GS
authors.lastname: Leiserowitz
authors.forename: David
authors.initials: D
authors.lastname: Gandara
authors.forename: Carlito B
authors.initials: CB
authors.lastname: Lebrilla
authors.forename: Kyoungmi
authors.initials: K
authors.lastname: Kim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D002853:Chromatography, Liquid / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008904:Miniaturization; D011134:Polysaccharides / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011134:Polysaccharides