title: Proteomics reveal cucumber Spd-responses under normal condition and salt stress.
abstract: To investigate the effects of exogenous Spd on proteomic changes under normal condition and NaCl stress of 3 days in cucumber seedling leaves, a 2-DE gel electrophoresis and MALDI-TOF/TOF MS was performed. A total of 63 differentially expressed proteins responded to salt stress or exogenous Spd treatments, and they were all successfully identified by MALDI-TOF/TOF MS. Many changes were observed in the levels of proteins involved in energy and metabolic pathways, protein metabolic, stress defense, and other functional proteins. Increased salt tolerance by exogenous Spd would contribute to higher expressions of proteins involved in the SAMs metabolism, protein biosynthesis, and defense mechanisms on antioxidant and detoxification. Meanwhile, the regulation of Calvin cycle, protein folding assembly and the inhibition of protein proteolysis by Spd might play important roles in salt tolerance. This study provides insight that may facilitate a better understanding of the salt resistance by Spd in cucumber seedlings.
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China.
authors.forename: Lizhong
authors.initials: L
authors.lastname: He
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China. Electronic address: srguo@njau.edu.cn.
authors.forename: Shirong
authors.initials: S
authors.lastname: Guo
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China.
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Yan
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agriculture University, Nanjing 210095, PR China; Nanjing Agricultural University (Suqian), Academy of Protected Horticulture, Jiangsu, Suqian 223800, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
mesh_terms: D028462:Cucumis / Q000187:drug effects* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013095:Spermidine / Q000494:pharmacology*
chemical_list: D012965:Sodium Chloride; D013095:Spermidine
keywords: Cucumber; Proteomics; Salt stress; Spermidine