title: Preparation of chitosan/silk fibroin/hydroxyapatite porous scaffold and its characteristics in comparison to bi-component scaffolds.
abstract: Composite porous scaffolds have attracted extensive attention in the biomedical material field. The aim of this research was to prepare a novel tri-component composite porous scaffold and to evaluate its relevant properties. The porous scaffold was composed of chitosan (CS), silk fibroin (SF), and nanohydroxyapatite particles (nHA), which we named CS/SF/nHA scaffold and prepared via salt fractionation method combined with lyophilization. The porous structure was achieved using a porogen (salt), and the pore size was controlled by the size of porogen. To evaluate the characteristics of the tri-component scaffold, three bi-component scaffolds, CS/SF, CS/nHA, and SF/nHA, were simultaneously prepared for comparison. The scaffolds were subjected to morphological, micro-structural, and biodegradation analyses. Results demonstrated that all of the scaffolds had pore sizes of 100-300 μm and a porosity of 90.5-96.1%. The biodegradation characteristics of all scaffolds meet the requirements of good biomedical materials. The investigation of the mechanical properties showed that the tri-component scaffold has better properties than the bi-component scaffolds. The in vitro biocompatibility with osteoblast-like MG-63 cells showed that all the scaffolds are suitable for cell attachment and proliferation; however, the CS/SF/nHA composite porous scaffold is much more effective than the others.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
authors.forename: Xiao-Ni
authors.initials: XN
authors.lastname: Qi
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Mou
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005349:Fibroins; D048271:Chitosan; D017886:Durapatite
keywords: biocompatibility; biodegradation; composite porous scaffold; mechanical properties