title: Cell cycle-regulated protein abundance changes in synchronously proliferating HeLa cells include regulation of pre-mRNA splicing proteins.
abstract: Cell proliferation involves dramatic changes in DNA metabolism and cell division, and control of DNA replication, mitosis, and cytokinesis have received the greatest attention in the cell cycle field. To catalogue a wider range of cell cycle-regulated processes, we employed quantitative proteomics of synchronized HeLa cells. We quantified changes in protein abundance as cells actively progress from G1 to S phase and from S to G2 phase. We also describe a cohort of proteins whose abundance changes in response to pharmacological inhibition of the proteasome. Our analysis reveals not only the expected changes in proteins required for DNA replication and mitosis but also cell cycle-associated changes in proteins required for biological processes not known to be cell-cycle regulated. For example, many pre-mRNA alternative splicing proteins are down-regulated in S phase. Comparison of this dataset to several other proteomic datasets sheds light on global mechanisms of cell cycle phase transitions and underscores the importance of both phosphorylation and ubiquitination in cell cycle changes.
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
authors.forename: Karen R
authors.initials: KR
authors.lastname: Lane
authors.forename: Yanbao
authors.initials: Y
authors.lastname: Yu
authors.forename: Patrick E
authors.initials: PE
authors.lastname: Lackey
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.forename: William F
authors.initials: WF
authors.lastname: Marzluff
authors.forename: Jeanette Gowen
authors.initials: JG
authors.lastname: Cook
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation / Q000502:physiology; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012322:RNA Precursors / Q000378:metabolism; D054875:Ubiquitination / Q000502:physiology*
chemical_list: D020543:Proteome; D012322:RNA Precursors