title: Spatial and temporal control of laminin-332 and -511 expressions during hair morphogenesis.
abstract: Hair is one of the smallest organs, but has many important functions to mammals. Hair morphogenesis occurs through the reciprocal exchange of epithelial and mesenchymal signals. There are some reports about the expression of laminin-511 and -332 during hair morphogenesis, but are no reports of the chronological expression and function of laminin-511 and its counter regulator laminin-332 during hair morphogenesis. Our results of immunoblotting revealed that laminin-332 proteins were detected at stage 0 and downregulated during stage 1 to stage 2, and then recovered at stage 3. However, laminin α5 expression was constant throughout stages 0-3. According to the results of semi-quantitative RT-PCR, the mRNA expression of all laminin-332 subunits increased gradually from stage 0 to stage 2, while the mRNA expression of all laminin-511 subunits remained constant from stage 0 to stage 3. Our results suggest that the proper expression of laminin-332 and laminin-511 may regulate appropriate hair morphogenesis.
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Imanishi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tsuruta
authors.forename: Chiharu
authors.initials: C
authors.lastname: Tateishi
authors.forename: Koji
authors.initials: K
authors.lastname: Sugawara
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Ishii
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kishi
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006197:Hair / Q000196:embryology* / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D039422:Integrin alpha3 / Q000378:metabolism; D039663:Integrin beta4 / Q000378:metabolism; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008813:Mice, Inbred ICR; D009024:Morphogenesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D039422:Integrin alpha3; D039663:Integrin beta4; D007797:Laminin; D000097794:Kalinin; C531281:laminin-511, mouse