title: Hosting the plant cells in vitro: recent trends in bioreactors.
abstract: Biotechnological production of high-value metabolites and therapeutic proteins by plant in vitro systems has been considered as an attractive alternative of classical technologies. Numerous proof-of-concept studies have illustrated the feasibility of scaling up plant in vitro system-based processes while keeping their biosynthetic potential. Moreover, several commercial processes have been established so far. Though the progress on the field is still limited, in the recent years several bioreactor configurations has been developed (e.g., so-called single-use bioreactors) and successfully adapted for growing plant cells in vitro. This review highlights recent progress and limitations in the bioreactors for plant cells and outlines future perspectives for wider industrialization of plant in vitro systems as "green cell factories" for sustainable production of value-added molecules.
authors.affiliation: Department of Applied Biotechnologies, Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria. milengeorgiev@gbg.bg
authors.forename: Milen I
authors.initials: MI
authors.lastname: Georgiev
authors.forename: Regine
authors.initials: R
authors.lastname: Eibl
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Zhong
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D059828:Plant Cells