title: Synthesis and characterization of a poly(lactic-co-glycolic acid) core + poly(N-isopropylacrylamide) shell nanoparticle system.
abstract: Poly(lactic-co-glycolic acid) (PLGA) is a popular material used to prepare nanoparticles for drug delivery. However, PLGA nanoparticles lack desirable attributes including active targeting abilities, resistance to aggregation during lyophilization, and the ability to respond to dynamic environmental stimuli. To overcome these issues, we fabricated a nanoparticle consisting of a PLGA core encapsulated within a shell of poly(N-isopropylacrylamide). Dynamic light scattering and transmission electron microscope imaging were used to characterize the nanoparticles, while an MTT assay and ELISA suggested biocompatibility in THP1 cells. Finally, a collagen type II binding assay showed successful modification of these nanoparticles with an active targeting moiety.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Kosinski
authors.forename: Jamie L
authors.initials: JL
authors.lastname: Brugnano
authors.forename: Brandon L
authors.initials: BL
authors.lastname: Seal
authors.forename: Frances C
authors.initials: FC
authors.lastname: Knight
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D002460:Cell Line; D024043:Collagen Type II / Q000737:chemistry; D016503:Drug Delivery Systems; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D008027:Light; D046529:Microscopy, Electron, Transmission; D009000:Monocytes / Q000166:cytology; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011485:Protein Binding; D012542:Scattering, Radiation; D013696:Temperature; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D024043:Collagen Type II; D010455:Peptides; D013778:Tetrazolium Salts; D013844:Thiazoles; D014409:Tumor Necrosis Factor-alpha; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C052970:poly-N-isopropylacrylamide; D011100:Polyglycolic Acid; D019344:Lactic Acid; C022616:thiazolyl blue
keywords: core-shell; functionalization of polymers; nanoparticles; poly(N-isopropylacrylamide) (pNIPAM); poly(lactic-co-glycolic acid) (PLGA)