title: Evaluation of the diagnostic efficacy of Fasciola adult worm vomit for serodiagnosis of human fasciolosis.
abstract: The diagnostic efficacy of Fasciola gigantica adult worm vomit (AWV) preparation in diagnosis of human fasciolosis was evaluated using conventional enzyme-linked immunosorbent assay (ELISA) and Falcon assay screening test (FAST)-ELISA in comparison with F. gigantica adult total soluble extract (TSE). Sera of fasciolosis patients, patients with other parasitic diseases (hydatid disease, schistosomiasis, toxoplasmosis, and amebiasis), and sera of healthy individuals were enrolled in this study. The results showed that the sensitivity of both conventional ELISA and FAST-ELISA was improved from 95 % using TSE to 100 % when using AWV. The specificity of conventional ELISA was 93.3 % using TSE and increased to 96.7 % using AWV. The specificity of FAST-ELISA using TSE was 96.7 % and became 100 % AWV antigen. The diagnostic accuracy of conventional ELISA was 94 % using TSE and increased to 98 % using AWV. The diagnostic accuracy of FAST-ELISA was 96 % using TSE and increased to 100 % using AWV. It is concluded that both TSE and AWV antigenic preparations are efficient for use in the serodiagnosis of human fasciolosis.
authors.affiliation: Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, 11566, Egypt.
authors.forename: Mahmoud Mohamed
authors.initials: MM
authors.lastname: Elsibaei
authors.forename: Nehad Mahmoud
authors.initials: NM
authors.lastname: Ali
authors.forename: Ayman Nabil
authors.initials: AN
authors.lastname: Ibrahim
authors.forename: Basant Osama
authors.initials: BO
authors.lastname: Mohammed
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D005766:Gastrointestinal Contents / Q000737:chemistry; D006801:Humans; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth