title: Atomic force microscopy for protein nanotechnology.
abstract: This chapter introduces atomic force microscopy (AFM) as an important tool for protein nanotechnology. A short review of AFM-based imaging, mapping, and spectroscopy of protein samples is given. AFM imaging of β-lactoglobulin nanofibrils in air is demonstrated. Basic concepts of AFM are described. Protocols for β-lactoglobulin nanofibrils and multiwall carbon nanotubes (MWCNT) samples preparation are defined. The operation of the microscope is described using MWCNT and the NanoScope E instrument in contact mode as an example. Nanostructure manipulation based on AFM nano-sweeping is demonstrated.
authors.affiliation: Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Sokolov
mesh_terms: D000818:Animals; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007782:Lactoglobulins / Q000737:chemistry / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000379:methods*; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D012996:Solutions; D013499:Surface Properties; D014584:User-Computer Interface
chemical_list: D007782:Lactoglobulins; D037742:Nanotubes, Carbon; D012996:Solutions