title: Zuo Jin Wan, a Traditional Chinese Herbal Formula, Reverses P-gp-Mediated MDR In Vitro and In Vivo.
abstract: Zuo Jin Wan (ZJW), a typical traditional Chinese medicine (TCM) formula, has been identified to have anticancer activity in recent studies. In this study, we determined the underlying mechanism of ZJW in the reversal effect of multidrug resistance on colorectal cancer in vitro and in vivo. Our results showed that ZJW significantly enhanced the sensitivity of chemotherapeutic drugs in HCT116/L-OHP, SGC7901/DDP, and Bel/Fu MDR cells. Moreover, combination of chemotherapy with ZJW could reverse the drug resistance of HCT116/L-OHP cells, increase the sensitivity of HCT116/L-OHP cells to L-OHP, DDP, 5-Fu, and MMC in vitro, and inhibit the tumor growth in the colorectal MDR cancer xenograft model. ICP-MS results showed that ZJW could increase the concentration of chemotherapeutic drugs in HCT116/L-OHP cells in a dose-dependent manner. Furthermore, we showed that ZJW could reverse drug resistance of colorectal cancer cells by decreasing P-gp level in vitro and in vivo, which has been represented as one of the major mechanisms that contribute to the MDR phenotype. Our study has provided the first direct evidence that ZJW plays an important role in reversing multidrug resistance of human colorectal cancer and may be considered as a useful target for cancer therapy.
authors.affiliation: Department of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China ; Interventional Cancer Institute of Integrative Medicine & Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Sui
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Bao-Hui
authors.initials: BH
authors.lastname: Jin
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Pan
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhou
authors.forename: Nikitin Alexander
authors.initials: NA
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Cai
authors.forename: Zhong-Ze
authors.initials: ZZ
authors.lastname: Fan
authors.forename: Hui-Rong
authors.initials: HR
authors.lastname: Zhu
authors.forename: Qi
authors.initials: Q
authors.lastname: Li