title: A magnetically separable biocatalyst for resolution of racemic naproxen methyl ester.
abstract: Candida rugosa lipase (CRL) was encapsulated via the sol-gel method, using 5, 11, 17, 23-tetra-tert-butyl-25,27-bis(2-aminopyridine)carbonylmethoxy-26, 28-dihydroxy-calix[4]arene-grafted magnetic Fe3O4 nanoparticles (Calix-M-E). The catalytic activity of encapsulated lipase (Calix-M-E) was tested both in the hydrolysis of p-nitrophenyl palmitate (p-NPP) and the enantioselective hydrolysis of racemic naproxen methyl ester. The present study demonstrated that the calixarene-based compound has the potential to enhance both reaction rate and enantioselectivity of the lipase-catalyzed hydrolysis of racemic naproxen methyl ester. The encapsulated lipase (Calix-M-E) had great catalytic activity and enantioselectivity (E > 400), as well as remarkable reusability as compared to the encapsulated lipase without supports (E = 137) for S-Naproxen.
authors.affiliation: Department of Chemistry, Faculty of Science, Selcuk University, 42075, Konya, Turkey, eyilmaz80@gmail.com.
authors.forename: Elif
authors.initials: E
authors.lastname: Ozyilmaz
authors.forename: Serkan
authors.initials: S
authors.lastname: Sayin
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000201:enzymology; D008049:Lipase / Q000737:chemistry; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D009288:Naproxen / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: C487872:naproxen methyl esther; D009288:Naproxen; D008049:Lipase