title: Which bovine endometrial cells are the source of and target for lysophosphatidic acid?
abstract: The objective of the study was to examine which cultured endometrial cells are the source and which are the target for lysophosphatidic acid (LPA) in the bovine uterus. LPA concentration as well as mRNA and protein expressions of the enzymes responsible for LPA synthesis (phospholipase A2: PLA2, autotaxin: AX) were greater in epithelial than in stromal cells (P<0.05). In turn, mRNA and protein expression of LPA receptor (LPAR1) was lower in epithelial than in stromal cells (P<0.05). We suggest that LPA in bovine endometrium is produced mainly by epithelial cells and affects mostly stromal cells acting via LPAR1.
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Boruszewska
authors.forename: Ilona
authors.initials: I
authors.lastname: Kowalczyk-Zieba
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Piotrowska-Tomala
authors.forename: Jean Sebastien
authors.initials: JS
authors.lastname: Saulnier-Blache
authors.forename: Tomas
authors.initials: T
authors.lastname: Acosta
authors.forename: Dariusz Jan
authors.initials: DJ
authors.lastname: Skarzynski
authors.forename: Izabela
authors.initials: I
authors.lastname: Woclawek-Potocka
mesh_terms: D000818:Animals; D002417:Cattle; D004717:Endometrium / Q000166:cytology* / Q000201:enzymology*; D030762:Estrous Cycle / Q000378:metabolism; D005260:Female; D008246:Lysophospholipids / Q000096:biosynthesis*; D054467:Phospholipases A2 / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid / Q000378:metabolism*; D017154:Stromal Cells / Q000201:enzymology; D000099189:Lysophospholipase D
chemical_list: D008246:Lysophospholipids; D054467:Phospholipases A2; D010727:Phosphoric Diester Hydrolases; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; D000099189:Lysophospholipase D; C032881:lysophosphatidic acid