title: Silencing SATB1 inhibits proliferation of human osteosarcoma U2OS cells.
abstract: It has been shown that over-expression of Special AT-rich binding protein 1 (SATB1) in breast cancer predicts a poor prognosis. This study was aimed at investigating the effects of silencing SATB1 on mesenchymal derived human osteosarcoma U2OS cells and the underlying mechanisms. The expressions of SATB1 and the related genes in the cells were detected by qRT-PCR and/or Western Blotting. SATB1 silencing was achieved by stable transfection with the vectors expressing small hairpin RNA versus SATB1. Cell proliferation was detected in a microplate reader with Cell Counting Kit-8 and the cell cycle was analyzed by flow cytometry using a cell cycle detection kit. The study found that SATB1 was particularly over-expressed in human osteosarcoma U2OS. Silencing SATB1 inhibited the proliferation of U2OS. It was found that inhibition of cell proliferation resulted from cell cycle arrest due to down-regulated expression of CFGF and JunB. The over-expression of SATB1 is responsible for abnormal proliferation of mesenchymal derived human Osteosatcoma U2OS cells, indicating that silencing SATB1 expression in the cells might be developed as an efficient osteosarcoma therapy. CTGF and JunB were involved in SATB1-mediated proliferation of U2OS cells.
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Qu
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
authors.forename: Yimin
authors.initials: Y
authors.lastname: Wang
authors.forename: Zonggui
authors.initials: Z
authors.lastname: Wang
authors.forename: Ronggui
authors.initials: R
authors.lastname: Li
mesh_terms: D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics* / Q000378:metabolism; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism; D012516:Osteosarcoma; D011471:Prostatic Neoplasms; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C526317:CCN2 protein, human; C581727:JunB protein, human; C558319:MED19 protein, human; D036961:Matrix Attachment Region Binding Proteins; D056892:Mediator Complex; D034741:RNA, Small Interfering; C076189:SATB1 protein, human; D014157:Transcription Factors; D055513:Connective Tissue Growth Factor