title: Two-electrode voltage clamp.
abstract: Two-electrode voltage clamp (TEVC) is a conventional electrophysiological technique used to artificially control the membrane potential (V m) of large cells to study the properties of electrogenic membrane proteins, especially ion channels. It makes use of two intracellular electrodes-a voltage electrode as V m sensor and a current electrode for current injection to adjust the V m, thus setting the membrane potential at desired values and recording the membrane current to analyze ion channel activities. Here we describe the use of TEVC in combination with exogenous mRNA expression in Xenopus oocytes for ion channel recording.
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, Hebei Province, China.
authors.forename: Bingcai
authors.initials: B
authors.lastname: Guan
authors.forename: Xingjuan
authors.initials: X
authors.lastname: Chen
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004566:Electrodes; D055724:Electrophysiological Phenomena; D051658:KCNQ2 Potassium Channel / Q000378:metabolism; D051659:KCNQ3 Potassium Channel / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques / Q000295:instrumentation*; D014981:Xenopus
chemical_list: D051658:KCNQ2 Potassium Channel; D051659:KCNQ3 Potassium Channel