title: Polymer coated liposomes for dental drug delivery--interactions with parotid saliva and dental enamel.
abstract: The interactions between pectin coated liposomes and parotid saliva and dental enamel were studied to investigate their potential to mimic the protective biofilm formed naturally on tooth surfaces. Different pectin coated liposomes with respect to pectin type (LM-, HM- and AM-pectin) and concentration (0.05% and 0.2%) were prepared. Interactions between the pectin coated liposomes and parotid saliva were studied by turbidimetry and imaging by atomic force microscopy. The liposomes were adsorbed to hydroxyapatite (HA) and human dental enamel using phosphate buffer and parotid saliva as adsorption media. A continuous flow was imposed on the enamel surfaces for various time intervals to examine their retention on the dental enamel. The results were compared to uncoated, charged liposomes. No aggregation tendencies for the pectin coated liposomes and parotid saliva were revealed. This makes them promising as drug delivery systems to be used in the oral cavity. In phosphate buffer the adsorption to HA of pectin coated liposomes was significantly lower than the negative liposomes. The difference diminished in parotid saliva. Positive liposomes adsorbed better to the dental enamel than the pectin coated liposomes. However, when subjected to flow for 1h, no significant differences in the retention levels on the enamel were found between the formulations. For all formulations, more than 40% of the liposomes still remained on the enamel surfaces. At time point 20 min the retention of HM-pectin coated and positive liposomes were significantly higher. It was concluded that pectin coated liposomes can adsorb to HA as well as to the dental enamel. Their ability to retain on the enamel surfaces promotes the concept of using them as protective structures for the teeth.
authors.affiliation: Department of Pharmacy, School of Pharmacy, University of Oslo, Sem Saelands vei 3, 0371 Oslo, Norway. sanko.nguyen@utoledo.edu
authors.forename: S
authors.initials: S
authors.lastname: Nguyen
authors.forename: M
authors.initials: M
authors.lastname: Hiorth
authors.forename: M
authors.initials: M
authors.lastname: Rykke
authors.forename: G
authors.initials: G
authors.lastname: Smistad
mesh_terms: D000284:Administration, Oral; D000327:Adsorption; D003743:Dental Enamel / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D016503:Drug Delivery Systems; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D018625:Microscopy, Atomic Force; D009391:Nephelometry and Turbidimetry; D010306:Parotid Gland / Q000378:metabolism*; D010368:Pectins / Q000737:chemistry* / Q000494:pharmacology; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D012463:Saliva / Q000737:chemistry*; D013499:Surface Properties; D014070:Tooth / Q000737:chemistry / Q000187:drug effects
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D004364:Pharmaceutical Preparations; D010368:Pectins; D017886:Durapatite
keywords: Adsorption; Dental enamel; Hydroxyapatite; Liposomes; Pectin; Saliva