title: Detection of Mycobacterium avium subspecies paratuberculosis by IS900-based PCR assays from an alpaca (Vicugna pacos) kept in a German zoological garden.
abstract: An alpaca (Vicugna pacos) bred and kept in a German zoological garden exhibited clinical signs consistent with paratuberculosis. The presence of Mycobacterium avium ssp. paratuberculosis (MAP) was confirmed in feces and in the ileocecal lymph node (ILN) by IS900-based polymerase chain reaction (PCR) assays and culture. A bacterial burden of 7.6 x 10(6) MAP/g in feces and 4.4 x 10(7) MAP/g in lymph node tissue was determined by real-time PCR. For further characterization, a conventional PCR was developed. After sequencing of the 864-bp PCR amplicon covering nucleotide positions 13 to 876 within the IS900, the alpaca isolate shared 100% nucleotide homology with the bovine MAP-K10 IS900 reference sequence (GenBank: AE16958), indicating a cattle strain. This report supports the present occurrence of MAP in German camelid populations and highlights the need to expand routine MAP surveillance to South American camelids held in European zoos.
authors.affiliation: Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August-University, Burckhardtweg 2, Göttingen, Lower Saxony, D-37077, Germany.
authors.forename: Pia
authors.initials: P
authors.lastname: Münster
authors.forename: Inger
authors.initials: I
authors.lastname: Völkel
authors.forename: Aloys
authors.initials: A
authors.lastname: von Buchholz
authors.forename: Claus-Peter
authors.initials: CP
authors.lastname: Czerny
mesh_terms: D000818:Animals; D000836:Animals, Zoo; D002161:Camelids, New World; D005858:Germany / Q000453:epidemiology; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*