title: Activation of the MEK pathway is required for complete scattering of MCF7 cells stimulated with heregulin-β1.
abstract: Rac1 is important for dissociation of cells during scattering, but whether its activation alone is sufficient to induce complete scattering is not known. To test this, we created an inducible MCF7 cell line that expresses dominant active Rac1. Although induction of dominant active Rac1 resulted in dissociation of cells, their scattering was incomplete. We co-expressed dominant active MKK1a, an activator of ERK, and dominant active Rac1. In this case, cells completely scattered. These results suggest that not only Rac1 but also the MEK1 pathway is required for dissociation and complete scattering of MCF7 cells treated with HRG-β1.
authors.affiliation: Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan, ROC.
authors.forename: Yu-Tsu
authors.initials: YT
authors.lastname: Chang
authors.forename: Chung-Li
authors.initials: CL
authors.lastname: Shu
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Fukui
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000256:Adenoviridae / Q000235:genetics; D022005:Adherens Junctions / Q000187:drug effects / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D005822:Genetic Vectors; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D048369:MAP Kinase Kinase 1 / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D020890:Neuregulin-1 / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D059008:Time-Lapse Imaging; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D006023:Glycoproteins; D020890:Neuregulin-1; C107953:RAC1 protein, human; C087230:heregulin beta1; D048051:p38 Mitogen-Activated Protein Kinases; D048369:MAP Kinase Kinase 1; C482125:MAP2K1 protein, human; D020830:rac1 GTP-Binding Protein