title: A microRNA encoded by HSV-1 inhibits a cellular transcriptional repressor of viral immediate early and early genes.
abstract: Viral microRNAs are one component of the RNA interference phenomenon generated during viral infection. They were first identified in the Herpesviridae family, where they were found to regulate viral mRNA translation. In addition, prior work has suggested that Kaposi's sarcoma-associated herpesvirus (KSHV) is capable of regulating cellular gene transcription by miRNA. We demonstrate that a miRNA, hsv1-mir-H27, encoded within the genome of herpes simplex virus 1 (HSV-1), targets the mRNA of the cellular transcriptional repressor Kelch-like 24 (KLHL24) that inhibits transcriptional efficiency of viral immediate-early and early genes. The viral miRNA is able to block the expression of KLHL24 in cells infected by HSV-1. Our discovery reveals an effective viral strategy for evading host cell defenses and supporting the efficient replication and proliferation of HSV-1.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wu
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Guo
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liao
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Lichun
authors.initials: L
authors.lastname: Wang
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D017874:Immediate-Early Proteins; C584157:KLHL24 protein, human; C584156:MIRN-H27 microRNA, herpes simplex virus 1; D035683:MicroRNAs; D012333:RNA, Messenger; D012367:RNA, Viral; D012097:Repressor Proteins; D014764:Viral Proteins