title: PPAR-γ agonist pioglitazone prevents apoptosis of endothelial progenitor cells from rat bone marrow.
abstract: Selective peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist affects the functions of endothelial progenitor cells (EPCs). This study explores the effect of selective PPAR-γ agonist, pioglitazone, on EPC apoptosis. The cells were cultured and identified via the double staining method in a medium containing different concentrations of pioglitazone. EPC apoptosis was detected by flow cytometry. On Day 7, EPCs engulfed DiL-ac-LDL and FITC-UEA-1, and showed yellow fluorescence in a laser-scanning confocal microscope. EPC apoptosis inhibition was maximal at 50 µmol/L. The ability of pioglitazone to prevent EPC apoptosis may be mediated by the PI3K/Akt signal pathway. The use of thiazolidine two ketone (TZD) to reduce EPC apoptosis may have some potential in treating vascular diseases.
authors.affiliation: The Second Department of Endocrinology, Henan Provincial People's Hospital, Zhengzhou Henan Province, China.
authors.forename: Hui-Feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Yuan
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Ma
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Wang
authors.forename: Zi-Ying
authors.initials: ZY
authors.lastname: Hu
authors.forename: Yong
authors.initials: Y
authors.lastname: Su
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D047495:PPAR gamma / Q000819:agonists* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000077205:Pioglitazone; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D045162:Thiazolidinediones / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D005456:Fluorescent Dyes; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D045162:Thiazolidinediones; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D000077205:Pioglitazone