title: BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause.
abstract: BACKGROUND
  The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats.
  
  
  RESULTS
  Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P < 0.001). After 3 months, the apoptosis rate in the BMSC group was significantly lower than that of the control group (P = 0.007).
  
  
  CONCLUSIONS
  BMSC therapy may protect against GC apoptosis induced by cisplatin and perimenopause. Further studies are necessary to evaluate therapeutic efficacy of BMSCs.
authors.forename: Jun-Qi
authors.initials: JQ
authors.lastname: Guo
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Wei-Zhen
authors.initials: WZ
authors.lastname: Wu
authors.forename: Liang-Hu
authors.initials: LH
authors.lastname: Huang
authors.forename: Hui-Yue
authors.initials: HY
authors.lastname: Dong
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Yun-Fen
authors.initials: YF
authors.lastname: Fu
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Ma
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Chen
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Wu
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: He
authors.forename: Shun-Liang
authors.initials: SL
authors.lastname: Yang
authors.forename: Lian-Ming
authors.initials: LM
authors.lastname: Liao
authors.forename: Feng
authors.initials: F
authors.lastname: Zheng
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Tan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D002945:Cisplatin / Q000008:administration & dosage*; D018920:Coculture Techniques / Q000379:methods*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D004967:Estrogens / Q000008:administration & dosage; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006107:Granulosa Cells / Q000187:drug effects* / Q000502:physiology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D047648:Perimenopause / Q000502:physiology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D004967:Estrogens; D016271:Proto-Oncogene Proteins c-myc; D051028:bcl-2-Associated X Protein; D002945:Cisplatin