title: Expression patterns of chemokine receptors on circulating T cells from myelodysplastic syndrome patients.
abstract: Chemokines and their receptors are involved in the recruitment of leukocytes to sites of inflammation. Recently, chemokine expression signatures have been reported to convey a prognostic value in myelodysplastic syndrome (MDS) patients. In the present study, we investigated the chemokine receptor repertoire on fresh peripheral blood lymphocytes from 31 (22 low-risk and 9 high-risk) patients affected by MDS. Chemokine receptor expression was studied in defined T-cell subsets using eight-color flow cytometry. MDS patients exhibited quantitative differences in peripheral lymphocyte subpopulations. In addition, T cells obtained from MDS patients expressed a chemokine receptor pattern suggesting a dominance of mature and activated T cells. This is illustrated by increased levels of CCR3, CCR5, CX3CR1 and/or by a decreased abundance of CCR7 in defined T-cell subsets. The T-cell subset distribution appears to differ between the peripheral blood and the bone marrow of MDS patients, suggesting a preferential recruitment of specific T-cell subsets to the latter compartment. Alteration in chemokine receptor expression can develop over time even in patients that are considered clinically stable. Elevated expression levels of CXCR4 by CD8+cells were associated with prolonged patient survival and reduced numbers of bone marrow blasts. We conclude that immunological abnormalities in MDS also involve chemokine receptors on different subsets of T cells, and that these changes may have a prognostic value.
authors.affiliation: Institute of Medicine; University of Bergen; Bergen, Norway.
authors.forename: Kristoffer Evebø
authors.initials: KE
authors.lastname: Sand
authors.forename: Kristin Paulsen
authors.initials: KP
authors.lastname: Rye
authors.forename: Bård
authors.initials: B
authors.lastname: Mannsåker
authors.forename: Oystein
authors.initials: O
authors.lastname: Bruserud
authors.forename: Astrid Olsnes
authors.initials: AO
authors.lastname: Kittang
keywords: T cells; chemokines; myelodysplastic syndrome