title: Removal characteristics and kinetic analysis of an aerobic vapor-phase bioreactor for hydrophobic alpha-pinene.
abstract: Biofiltration is considered an effective method to control volatile organic compounds (VOCs) pollution. This study was conducted to evaluate the potential use of a bacterial biofilter packed with wood chips and peat for the removal of hydrophobic alpha-pinene. When inoculated with two pure degraders and adapted activated sludge, a removal efficiency (RE) of more than 95% was achieved after a startup period of 11 days. The maximum elimination capacity (EC) of 50 g/(m3 x hr) with RE of 94% was obtained at empty bed retention time (EBRT) of 102 sec. When higher alpha-pinene concentrations and shorter EBRTs were applied, the REs and ECs decreased significantly due to mass-transfer and biological reaction limitations. As deduced from the experimental results, approximately 74% of alpha-pinene were completely mineralized by the consortiums and the biomass yield was 0.60 g biomass/g alpha-pinene. Sequence analysis of the selected bands excised from denaturing gradient gel electrophoresis revealed that the inoculated pure cultures could be present during the whole operation, and others were closely related to bacteria being able to degrade hydrocarbons. The kinetic results demonstrated that the whole biofiltration for alpha-pinene was diffusion-limit controlled owing to its hydrophobic characteristics. These findings indicated that this bacterial biofiltration is a promising technology for the remediation of hydrophobic industrial waste gases containing alpha-pinene.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. jyf@zjut.edu.cn
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Jiang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Zhuowei
authors.initials: Z
authors.lastname: Cheng
authors.forename: Runye
authors.initials: R
authors.lastname: Zhu
authors.forename: Jianmeng
authors.initials: J
authors.lastname: Chen
mesh_terms: D000393:Air Pollutants / Q000302:isolation & purification*; D000397:Air Pollution / Q000517:prevention & control*; D000081008:Bicyclic Monoterpenes; D019149:Bioreactors / Q000382:microbiology; D002245:Carbon Dioxide / Q000032:analysis; D058645:Denaturing Gradient Gel Electrophoresis; D007700:Kinetics; D059013:Microbial Consortia; D039821:Monoterpenes / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011551:Pseudomonas fluorescens / Q000378:metabolism; D055549:Volatile Organic Compounds / Q000302:isolation & purification*
chemical_list: D000393:Air Pollutants; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D055549:Volatile Organic Compounds; D002245:Carbon Dioxide; C005451:alpha-pinene