title: A liaF codon deletion abolishes daptomycin bactericidal activity against vancomycin-resistant Enterococcus faecalis.
abstract: The genetic bases for antibiotic tolerance are obscure. Daptomycin (DAP) is a lipopeptide antibiotic with bactericidal activity against enterococci. Using time-kill assays, we provide evidence for the first time that a deletion of isoleucine in position 177 of LiaF, a member of the three-component regulatory system LiaFSR involved in the cell envelope response to antimicrobials, is directly responsible for a DAP-tolerant phenotype and is likely to negatively affect response to DAP therapy.
authors.affiliation: Laboratory for Antimicrobial Research, University of Texas Medical School at Houston, Houston, Texas, USA.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Munita
authors.forename: Truc T
authors.initials: TT
authors.lastname: Tran
authors.forename: Lorena
authors.initials: L
authors.lastname: Diaz
authors.forename: Diana
authors.initials: D
authors.lastname: Panesso
authors.forename: Jinnethe
authors.initials: J
authors.lastname: Reyes
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Murray
authors.forename: Cesar A
authors.initials: CA
authors.lastname: Arias
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002473:Cell Wall / Q000187:drug effects; D003062:Codon / Q000235:genetics*; D017576:Daptomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016984:Enterococcus faecium / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000592:standards; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics*; D013234:Stem Cells; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003062:Codon; D014640:Vancomycin; D017576:Daptomycin