title: CD36 is important for adipocyte recruitment and affects lipolysis.
abstract: OBJECTIVE
  The scavenger receptor CD36 facilitates the cellular uptake of long-chain fatty acids. As CD36-deficiency attenuates the development of high fat diet (HFD)-induced obesity, the role of CD36-deficiency in preadipocyte recruitment and adipocyte function was set out to characterize.
  
  
  METHODS
  Fat cell size and number were determined in gonadal, visceral, and subcutaneous adipose tissue of CD36(-/-) and WT mice after 6 weeks on HFD. Basal lipolysis and insulin-inhibited lipolysis were investigated in gonadal adipose tissue.
  
  
  RESULTS
  CD36(-/-) mice showed a reduction in adipocyte size in all fat pads. Gonadal adipose tissue also showed a lower total number of adipocytes because of a lower number of very small adipocytes (diameter <50 μm). This was accompanied by an increased pool of preadipocytes, which suggests that CD36-deficiency reduces the capacity of preadipocytes to become adipocytes. Regarding lipolysis, in adipose tissue from CD36(-/-) mice, cAMP levels were increased and both basal and 8-bromo-cAMP stimulated lipolysis were higher. However, insulin-mediated inhibition of lipolysis was more potent in CD36(-/-) mice.
  
  
  CONCLUSIONS
  These results indicate that during fat depot expansion, CD36-deficiency negatively affects preadipocyte recruitment and that in mature adipocytes, CD36-deficiency is associated with increased basal lipolysis and insulin responsiveness.
authors.affiliation: Department of General Internal Medicine, Endocrinology and Metabolic Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Irene O C M
authors.initials: IO
authors.lastname: Vroegrijk
authors.forename: Jan Bert
authors.initials: JB
authors.lastname: van Klinken
authors.forename: Janna A
authors.initials: JA
authors.lastname: van Diepen
authors.forename: Sjoerd A A
authors.initials: SA
authors.lastname: van den Berg
authors.forename: Maria
authors.initials: M
authors.lastname: Febbraio
authors.forename: Laura K M
authors.initials: LK
authors.lastname: Steinbusch
authors.forename: Jan F C
authors.initials: JF
authors.lastname: Glatz
authors.forename: Louis M
authors.initials: LM
authors.lastname: Havekes
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Voshol
authors.forename: Patrick C N
authors.initials: PC
authors.lastname: Rensen
authors.forename: Ko Willems
authors.initials: KW
authors.lastname: van Dijk
authors.forename: Vanessa
authors.initials: V
authors.lastname: van Harmelen
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000378:metabolism; D017667:Adipocytes / Q000502:physiology*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D018955:CD36 Antigens / Q000172:deficiency / Q000502:physiology*; D002454:Cell Differentiation; D048429:Cell Size; D059305:Diet, High-Fat / Q000009:adverse effects; D005227:Fatty Acids / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D008066:Lipolysis / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009765:Obesity / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D018955:CD36 Antigens; D005227:Fatty Acids; D007328:Insulin; D014280:Triglycerides; D015124:8-Bromo Cyclic Adenosine Monophosphate