title: Detoxification of malachite green by Pleurotus florida laccase produced under solid-state fermentation using agricultural residues.
abstract: Laccase was produced from Pleurotus florida under solid-state fermentation, and the production was optimized by response surface methodology. The predicted maximum laccase production of 8.81 U g(-1) was obtained by the optimum concentration of malt extract, banana peel, wheat bran and CuSO4, which was found to be 0.69 g, 10.61 g, 10.68 g and 77.15 ppm, respectively. The validation results suggested that the laccase production was 7.96 U g(-1) in the optimized medium, which was close to the predicted value. Decolorization efficiency of P. florida laccase was evaluated against malachite green (MG). Rapid decolorization of MG dye was observed, and a dark-coloured precipitate was formed in the reaction mixture. HPLC analysis indicated that the laccase enzyme degraded MG by the demethylation process. The toxicity of MG was reduced to 67% after the treatment with laccase, which was confirmed by a phytotoxicity study.
authors.affiliation: Laboratory of Bioprocess and Engineering, Department of Biochemistry, Periyar University, Salem, Tamil Nadu 636 011, India. pal2912@yahoo.com
authors.forename: Palanivel
authors.initials: P
authors.lastname: Sathishkumar
authors.forename: Thayumanavan
authors.initials: T
authors.lastname: Palvannan
authors.forename: Kumarasamy
authors.initials: K
authors.lastname: Murugesan
authors.forename: Seralathan
authors.initials: S
authors.lastname: Kamala-Kannan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018556:Crops, Agricultural / Q000378:metabolism*; D004586:Electrophoresis; D005285:Fermentation; D042845:Laccase / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology*; D012394:Rosaniline Dyes / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013053:Spectrophotometry
chemical_list: D012394:Rosaniline Dyes; C005095:malachite green; D042845:Laccase