title: Function prediction from networks of local evolutionary similarity in protein structure.
abstract: BACKGROUND
  Annotating protein function with both high accuracy and sensitivity remains a major challenge in structural genomics. One proven computational strategy has been to group a few key functional amino acids into templates and search for these templates in other protein structures, so as to transfer function when a match is found. To this end, we previously developed Evolutionary Trace Annotation (ETA) and showed that diffusing known annotations over a network of template matches on a structural genomic scale improved predictions of function. In order to further increase sensitivity, we now let each protein contribute multiple templates rather than just one, and also let the template size vary.
  
  
  RESULTS
  Retrospective benchmarks in 605 Structural Genomics enzymes showed that multiple templates increased sensitivity by up to 14% when combined with single template predictions even as they maintained the accuracy over 91%. Diffusing function globally on networks of single and multiple template matches marginally increased the area under the ROC curve over 0.97, but in a subset of proteins that could not be annotated by ETA, the network approach recovered annotations for the most confident 20-23 of 91 cases with 100% accuracy.
  
  
  CONCLUSIONS
  We improve the accuracy and sensitivity of predictions by using multiple templates per protein structure when constructing networks of ETA matches and diffusing annotations.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
authors.forename: Serkan
authors.initials: S
authors.lastname: Erdin
authors.forename: Eric
authors.initials: E
authors.lastname: Venner
authors.forename: Andreas Martin
authors.initials: AM
authors.lastname: Lisewski
authors.forename: Olivier
authors.initials: O
authors.lastname: Lichtarge
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D030562:Databases, Protein; D004798:Enzymes / Q000737:chemistry; D019143:Evolution, Molecular; D023281:Genomics; D058977:Molecular Sequence Annotation; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
chemical_list: D004798:Enzymes; D011506:Proteins