title: Wu-Chia-Pi solution attenuates carbon tetrachloride-induced hepatic injury through the antioxidative abilities of its components acteoside and quercetin.
abstract: Wu-Chia-Pi medicated wine, composed nine Chinese medicines soaked in 35% alcohol, is widely used in Asia for its health-promoting functions. However, long-term consumption of alcohol could result in liver dysfunction. In this study, Wu-Chia-Pi solution (WCPS) and extract (WCPE) were prepared by modification of the principals given by the Committee on Chinese Medicine and Pharmacy in Taiwan. The aim of this study was to explore the protective effect of WCPS against carbon tetrachloride (CCl4)-induced liver injury and to clarify its active component(s). Antioxidative effects of the test samples were evaluated via MDA inhibition, catalase activity and DPPH-scavenging assays. HPLC was used to analysis the active components. Results showed that WCPS (1 and 5 mL/kg) significantly prevented CCl4-induced liver injury without chronic liver toxicity. Referring to the antioxidative activities, WCPE displayed significant MDA inhibitory and DPPH-scavenging activities with IC50 values of 0.91 ± 0.03 and 0.60 ± 0.04 mg/mL, respectively. Catalase activity was also enhanced by treatment of WCPE, acteoside and quercetin. Therefore, we suggest that acteoside and quercetin are the major contributors to the antioxidative and hepatoprotective activities of WCPS, and a possible mechanism could be mediated through reduction of oxidative stress.
authors.affiliation: Division of Urology, Department of Surgery, Chi Mei Medical Center, No 21, Taikang, Liuying Dist, Tainan 73657, Taiwan. skhsteven@yahoo.com.tw
authors.forename: Steven Kuan-Hua
authors.initials: SK
authors.lastname: Huan
authors.forename: Kun-Teng
authors.initials: KT
authors.lastname: Wang
authors.forename: Chia-Jung
authors.initials: CJ
authors.lastname: Lee
authors.forename: Chun-Hsien
authors.initials: CH
authors.lastname: Sung
authors.forename: Ting-Yi
authors.initials: TY
authors.lastname: Chien
authors.forename: Ching-Chiung
authors.initials: CC
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000009:adverse effects*; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008107:Liver Diseases / Q000188:drug therapy / Q000517:prevention & control; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D013624:Taiwan; D059808:Polyphenols
chemical_list: D000975:Antioxidants; D002251:Carbon Tetrachloride; D002374:Catalase; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D011794:Quercetin; C058956:acteoside; D059808:Polyphenols