title: Maternal diabetes causes alterations of DNA methylation statuses of some imprinted genes in murine oocytes.
abstract: Maternal diabetes has adverse effects not only on oocyte quality but also on embryo development. However, it is still unknown whether the DNA imprinting in oocytes is altered by diabetes. By using streptozotocin (STZ)-induced and nonobese diabetic (NOD) mouse models we investigated the effect of maternal diabetes on DNA methylation of imprinted genes in oocytes. Mice which were judged as being diabetic 4 days after STZ injection were used for experiments. In superovulated oocytes of diabetic mice, the methylation pattern of Peg3 differential methylation regions (DMR) was affected in a time-dependent manner, and evident demethylation was observed on Day 35 after STZ injection. The expression level of DNA methyltransferases (DNMTs) was also decreased in a time-dependent manner in diabetic oocytes. However, the methylation patterns of H19 and Snrpn DMRs were not significantly altered by maternal diabetes, although there were some changes in Snrpn. In NOD mice, the methylation pattern of Peg3 was similar to that of STZ-induced mice. Embryo development was adversely affected by maternal diabetes; however, no evident imprinting abnormality was observed in oocytes from female offspring derived from a diabetic mother. These results indicate that maternal diabetes has adverse effects on DNA methylation of maternally imprinted gene Peg3 in oocytes of a diabetic female in a time-dependent manner, but methylation in offspring's oocytes is normal.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhao-Jia
authors.initials: ZJ
authors.lastname: Ge
authors.forename: Xing-Wei
authors.initials: XW
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Liang
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Luo
authors.forename: Ya-Peng
authors.initials: YP
authors.lastname: Wang
authors.forename: Yan-Chang
authors.initials: YC
authors.lastname: Wei
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Han
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D019175:DNA Methylation; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D015870:Gene Expression; D018392:Genomic Imprinting; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D009865:Oocytes / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D055517:snRNP Core Proteins
chemical_list: C094335:H19 long non-coding RNA; D051741:Kruppel-Like Transcription Factors; C097901:Peg3 protein, mouse; D062085:RNA, Long Noncoding; D055517:snRNP Core Proteins
keywords: imprinted gene; maternal diabetes mellitus; oocyte