title: [Effects of 17β-estrogen on expressions of C-reactive protein and its mRNA of vascular smooth muscle cells in rats].
abstract: To investigate the effects of 17β-estrogen on expressions of C-reactive protein (CRP) and its mRNA in vascular smooth muscle cells(VSMCs).
  
  
  
  Immunocytochemistry was used to detect CRP level in normal VSMCs. The expressions of C-reactive protein and p-ERK1/2 in Ang-II-stimulated VSMCs were evaluated with Western blot. C-reactive protein mRNA was examined with RT-PCR.
  
  
  
  17β-estrogen had no effect on cell morphology and C-reactive protein expression in normal VSMCs; however, C-reactive protein and mRNA, as well as p-ERK1/2 were decreased in Ang-II-stimulated VSMCs after 17β-estrogen treatment in a concentration-dependent manner.
  
  
  
  17β-estrogen may inhibit the expression of C-reactive protein and its mRNA in Ang-II-stimulated VSMCs via ERK1/2 signal transduction pathway in a concentration-dependent way.
authors.affiliation: Department of Pharmacy, the Affiliated Hospital of Xi'an Medical College, Xi'an, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Xinwu
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Pang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Kaige
authors.initials: K
authors.lastname: Liu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004967:Estrogens / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004967:Estrogens; D012333:RNA, Messenger; D002097:C-Reactive Protein