title: Selective chemical labelling of 5-formylcytosine in DNA by fluorescent dyes.
abstract: Direct labelling: 5-Formylcytosine in DNA can be selectively labelled by fluorescent dyes containing an active amino group. The labelled DNA shows strong fluorescence and can be detected by polyacrylamide gel electrophoresis (PAGE) and fluorescence measurements (see scheme). This method can distinguish 5-formylcytosine from other methylation forms of cytosine in DNA.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, PR China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Hu
authors.forename: Xiwen
authors.initials: X
authors.lastname: Xing
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.forename: Pu
authors.initials: P
authors.lastname: Guo
authors.forename: Shengyong
authors.initials: S
authors.lastname: Yan
authors.forename: Zhenghao
authors.initials: Z
authors.lastname: Xu
authors.forename: Jiahui
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D015415:Biomarkers; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004249:DNA Damage; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009705:Nucleosides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: C560973:5-formylcytosine; D015415:Biomarkers; D005456:Fluorescent Dyes; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D003596:Cytosine; D004247:DNA