title: Development of affinity-based delivery of NGF from a chondroitin sulfate biomaterial.
abstract: Chondroitin sulfate is a major component of the extracellular matrix in both the central and peripheral nervous systems. Chondroitin sulfate is upregulated at injury, thus methods to promote neurite extension through chondroitin sulfate-rich matrices and synthetic scaffolds are needed. We describe the use of both chondroitin sulfate and a novel chondroitin sulfate-binding peptide to control the release of nerve growth factor. Interestingly, the novel chondroitin sulfate-binding peptide enhances the controlled release properties of the chondroitin sulfate gels. While introduction of chondroitin sulfate into a scaffold inhibits primary cortical outgrowth, the combination of chondroitin sulfate, chondroitin sulfate-binding peptide and nerve growth factor promotes primary cortical neurite outgrowth in chondroitin sulfate gels.
authors.affiliation: Harrington Department of Bioengineering, Arizona State University, Tempe, AZ, USA.
authors.forename: Karen Chao
authors.initials: KC
authors.lastname: Butterfield
authors.forename: Aaron W
authors.initials: AW
authors.lastname: Conovaloff
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
mesh_terms: D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry; D001921:Brain / Q000196:embryology; D002809:Chondroitin Sulfates / Q000737:chemistry*; D016503:Drug Delivery Systems; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D020932:Nerve Growth Factor / Q000008:administration & dosage*; D009416:Nerve Regeneration; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D051381:Rats; D013431:Sulfates / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides; D013431:Sulfates; D011092:Polyethylene Glycols; D002809:Chondroitin Sulfates; D020932:Nerve Growth Factor
keywords: chondroitin sulfate; controlled release; hydrogel; nerve growth factor; nerve regeneration; spinal cord injury