title: Effect of cadmium on photosynthetic pigments, lipid peroxidation, antioxidants, and artemisinin in hydroponically grown Artemisia annua.
abstract: The effects of different cadmium (Cd) concentrations (0, 20, 60, and 100 micromol/L) on hydroponically grown Artemisia annua L. were investigated. Cd treatments applied for 0, 4, 12, 24, 72, 144, 216, and 336 hr were assessed by measuring the changes in photosynthetic pigments, electrolyte leakage, malondialdehyde (MDA) and antioxidants (ascorbic acid and glutathione), while the artemisinin content was tested after 0, 12, 144, 216, and 336 hr. A significant decrease was observed in photosynthetic pigment levels over time with increasing Cd concentration. Chlorophyll b levels were more affected by Cd than were chlorophyll a or carotenoid levels. The cell membrane was sensitive to Cd stress, as MDA content in all treatment groups showed insignificant differences from the control group, except at 12 hr treatment time. Ascorbic acid (AsA) content changed slightly over time, while glutathione (GSH) content took less time to reach a maximum as Cd concentration increased. Cd was found to promote synthesis and accumulation of artemisinin, especially at concentrations of 20 and 100 micromol/L. In conclusion, Cd stress can damage to photosynthetic pigments, and vigorously growing A. annua showed a strong tolerance for Cd stress. Appropriate amounts of added Cd aided synthesis and accumulation of artemisinin.
authors.affiliation: School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. lixuanlwj@hotmail.com
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Manxi
authors.initials: M
authors.lastname: Zhao
authors.forename: Lanping
authors.initials: L
authors.lastname: Guo
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D031172:Artemisia annua / Q000187:drug effects* / Q000378:metabolism; D037621:Artemisinins / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D004553:Electric Conductivity; D005978:Glutathione / Q000378:metabolism; D018527:Hydroponics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*
chemical_list: D037621:Artemisinins; D002104:Cadmium; D008315:Malondialdehyde; C031327:artemisinin; D005978:Glutathione; D001205:Ascorbic Acid