title: Eosinophil recruitment to nasal nerves after allergen challenge in allergic rhinitis.
abstract: In allergen challenged animal models, eosinophils localize to airway nerves leading to vagally-mediated hyperreactivity. We hypothesized that in allergic rhinitis eosinophils recruited to nasal nerves resulted in neural hyperreactivity. Patients with persistent allergic rhinitis (n=12), seasonal allergic rhinitis (n=7) and controls (n=10) were studied. Inferior nasal turbinate biopsies were obtained before, 8 and 48h after allergen challenge. Eight hours after allergen challenge eosinophils localized to nerves in both rhinitis groups; this was sustained through 48h. Bradykinin challenge, with secretion collection on the contralateral side, was performed to demonstrate nasal nerve reflexes. Twenty fourhours after allergen challenge, bradykinin induced a significant increase in secretions, indicating nasal hyperreactivity. Histological studies showed that nasal nerves expressed both vascular cell adhesion molecule-1 (VCAM-1) and chemokine (C-C motif) ligand 26 (CCL-26). Hence, after allergen challenge eosinophils are recruited and retained at nerves and so may be a mechanism for neural hyperreactivity.
authors.affiliation: Department of Otolaryngology, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Thornton
authors.affiliation: Department of Medicine, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland. Electronic address: nadimakasheh@physicians.ie.
authors.forename: Nadim
authors.initials: N
authors.lastname: Akasheh
authors.affiliation: Department of Medicine, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Marie-Therese
authors.initials: MT
authors.lastname: Walsh
authors.affiliation: Department of Medicine, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Michael
authors.initials: M
authors.lastname: Moloney
authors.affiliation: Department of Otolaryngology, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Patrick O
authors.initials: PO
authors.lastname: Sheahan
authors.affiliation: Department of Medicine, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Smyth
authors.affiliation: Department of Otolaryngology, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Rory McConn
authors.initials: RM
authors.lastname: Walsh
authors.affiliation: Department of Medicine, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Ross M
authors.initials: RM
authors.lastname: Morgan
authors.affiliation: Department of Medicine, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: David R
authors.initials: DR
authors.lastname: Curran
authors.affiliation: Department of Otolaryngology, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Walsh
authors.affiliation: Department of Dermatology, School of Medicine, Salt Lake City, Utah, 84132, USA.
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Gleich
authors.affiliation: Department of Medicine, Royal College of Surgeons in Ireland, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Costello
mesh_terms: D000485:Allergens / Q000276:immunology*; D000075906:Chemokine CCL26; D019742:Chemokines, CC / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000276:immunology* / Q000294:innervation / Q000378:metabolism; D009299:Nasal Provocation Tests; D009420:Nervous System / Q000276:immunology* / Q000378:metabolism; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology* / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology / Q000378:metabolism
chemical_list: D000485:Allergens; C120429:CCL26 protein, human; D000075906:Chemokine CCL26; D019742:Chemokines, CC; D019010:Vascular Cell Adhesion Molecule-1