title: The effects of engineered nanoparticles on the cellular structure and growth of Saccharomyces cerevisiae.
abstract: In order to study the effects of nanoparticles (NPs) with different physicochemical properties on cellular viability and structure, Saccharomyces cerevisiae were exposed to different concentrations of TiO2-NPs (1-3 nm), ZnO-NPs (<100 nm), CuO-NPs (<50 nm), their bulk forms, Ag-NPs (10 nm) and single-walled carbon nanotubes (SWCNTs). The GreenScreen assay was used to measure cyto- and genotoxicity, and transmission electron microscopy (TEM) used to assess ultrastructure. CuO-NPs were highly cytotoxic, reducing the cell density by 80% at 9 cm(2)/ml, and inducing lipid droplet formation. Cells exposed to Ag-NPs (19 cm(2)/ml) and TiO2-NPs (147 cm(2)/ml) contained dark deposits in intracellular vacuoles, the cell wall and vesicles, and reduced cell density (40 and 30%, respectively). ZnO-NPs (8 cm(2)/ml) caused an increase in the size of intracellular vacuoles, despite not being cytotoxic. SWCNTs did not cause cytotoxicity or significant alterations in ultrastructure, despite high oxidative potential. Two genotoxicity assays, GreenScreen and the comet assay, produced different results and the authors discuss the reasons for this discrepancy. Classical assays of toxicity may not be the most suitable for studying the effects of NPs in cellular systems, and the simultaneous assessment of other measures of the state of cells, such as TEM are highly recommended.
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University , Stockholm , Sweden.
authors.forename: Narges
authors.initials: N
authors.lastname: Bayat
authors.forename: Katarina
authors.initials: K
authors.lastname: Rajapakse
authors.forename: Romana
authors.initials: R
authors.lastname: Marinsek-Logar
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobne
authors.forename: Susana
authors.initials: S
authors.lastname: Cristobal
mesh_terms: D020552:Comet Assay; D053768:Metal Nanoparticles / Q000633:toxicity*; D050296:Microbial Viability / Q000187:drug effects; D009152:Mutagenicity Tests; D018384:Oxidative Stress / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D014025:Titanium / Q000633:toxicity*; D015034:Zinc Oxide / Q000633:toxicity*
chemical_list: C009495:titanium dioxide; D014025:Titanium; D015034:Zinc Oxide