title: ApoG2 induces ER stress-dependent apoptosis in gastric cancer cells in vitro and its real-time evaluation by bioluminescence imaging in vivo.
abstract: Apogossypolone (ApoG2), a potent small molecular inhibitor of Bcl-2 family proteins, is reported to have a significant anti-cancer effect in several types of cancers, but it has not been investigated in gastric cancer. In this study, we demonstrate in vitro and in vivo that ApoG2 inhibits human gastric cancer. Gastric carcinoma cell growth and proliferation was significantly hampered in vitro, as measured by MTT and colony formation assays. Real-time bioluminescence imaging indicated that ApoG2 causes tumor growth delay in a murine xenograft model. Further studies revealed that the ApoG2 induced apoptosis in gastric cancer cells was associated with the endoplasmic reticulum stress-induced apoptosis pathway. Conclusively, our results indicate that ApoG2 may be a promising agent for gastric cancer therapy.
authors.affiliation: School of Life Sciences and Technology, Xidian University, Xi'an 710071, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xin
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zhan
authors.forename: Muhan
authors.initials: M
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.forename: Limin
authors.initials: L
authors.lastname: Xia
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Jimin
authors.initials: J
authors.lastname: Liang
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005260:Female; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D017382:Reactive Oxygen Species; C492806:apogossypol; D006072:Gossypol; D002118:Calcium