title: Conventional micropipette-based patch clamp techniques.
abstract: The patch clamp technique revolutionized the study of ion channels and is considered the gold standard of measuring ion channel activity, from the academic laboratory to industrial-scale drug screening. This technique enables the study of ion channels, from single molecules up to the whole-cell ion channel population, and in their native environment. Whilst the study of single protein molecular behavior is the ultimate goal of biophysicists from all fields, this is a routine ability for ion channel specialists. This chapter is aimed at helping the beginner to design patch clamp experiments and to obtain the fundamental micropipette configurations with mammalian cells: cell-attached patch, whole cell, inside-out patch, and outside-out patch.
authors.affiliation: Faculty of Biological Sciences, School of Biomedical Sciences, University of Leeds, Leeds, UK.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Lippiat
authors.forename: David C
authors.initials: DC
authors.lastname: Wrighton
mesh_terms: D004553:Electric Conductivity; D057809:HEK293 Cells; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000378:metabolism; D008024:Ligands; D055616:Microtechnology / Q000379:methods*; D018408:Patch-Clamp Techniques / Q000379:methods*
chemical_list: D007473:Ion Channels; D008024:Ligands