title: Mixed electrolytes in hydrophobic interaction chromatography.
abstract: An essential part of the modulation of protein-binding capacity in hydrophobic interaction chromatography is the buffer-salt system. Besides using "single" electrolytes, multicomponent electrolyte mixtures may be used as an additional tool. Both the protein solubility and the binding capacity depend on the position of a salt in the so-called Hofmeister series. Specific interactions are observed for an individual protein-salt combination. For salt mixtures, selectivity, recovery, and binding capacity do not behave like for the single salts that are positioned in between the two mixed components in the Hofmeister series, as the continuous correlation would suggest. Thus, finding strategies for mixed salts could potentially lead to improved capacities in hydrophobic interaction chromatography. Mixtures of ammonium sulfate, sodium citrate, sodium sulfate, sodium chloride, sodium acetate, and glycine were used to investigate the binding capacities for lysozyme and a monoclonal antibody on various hydrophobic resins. Resin capacity for two investigated proteins increases when mixtures consisting of a chaotropic and a kosmotropic salt are applied. It seems to be related to the rather basic isoelectric points of the proteins.
authors.affiliation: TOSOH Bioscience GmbH, Stuttgart, Germany. egbert.mueller@tosoh.com
authors.forename: Egbert
authors.initials: E
authors.lastname: Müller
authors.forename: Judith
authors.initials: J
authors.lastname: Vajda
authors.forename: Djuro
authors.initials: D
authors.lastname: Josic
authors.forename: Tim
authors.initials: T
authors.lastname: Schröder
authors.forename: Romain
authors.initials: R
authors.lastname: Dabre
authors.forename: Tim
authors.initials: T
authors.lastname: Frey
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004573:Electrolytes / Q000737:chemistry*; D012995:Solubility; D013500:Surface Tension
chemical_list: D004573:Electrolytes