title: Purification, crystallization and preliminary crystallographic analysis of the marine α-amylase AmyP.
abstract: AmyP is a raw-starch-degrading α-amylase newly identified from a marine metagenome library. It shares low sequence similarity with characterized glycoside hydrolases and was classified into a new subfamily of GH13. In particular, it showed preferential degradation to raw rice starch. Full-length AmyP was cloned and overexpressed in Escherichia coli, then purified and crystallized in the presence of its substrate analogue β-cyclodextrin. X-ray diffraction data were collected to a resolution of 2.1 Å. The crystal belonged to space group P2₁2₁2, with unit-cell parameters a=129.824, b=215.534, c=79.699 Å, α=β=γ=90°, and was estimated to contain two molecules in one asymmetric unit.
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230039, People's Republic of China.
authors.forename: Jigang
authors.initials: J
authors.lastname: Yu
authors.forename: Chengliang
authors.initials: C
authors.lastname: Wang
authors.forename: Yanjin
authors.initials: Y
authors.lastname: Hu
authors.forename: Yuanqiu
authors.initials: Y
authors.lastname: Dong
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Tu
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Zhang
mesh_terms: D059001:Aquatic Organisms / Q000737:chemistry* / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D054892:Metagenome; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013213:Starch / Q000737:chemistry / Q000378:metabolism; D000516:alpha-Amylases / Q000737:chemistry* / Q000235:genetics; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D047392:beta-Cyclodextrins; D013213:Starch; D000516:alpha-Amylases; C031215:betadex
keywords: AmyP; α-amylase