title: Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves.
abstract: BACKGROUND
  Research on natural products has gained a wide popularity due to the potential of discovering active compounds. The antioxidant properties contained in plants have been proposed as one of the mechanisms for the observed beneficial effect. Therefore, the present study investigated the antioxidant activity and total phenolic contents of various solvent extracts of Albizia procera leaves.
  
  
  METHODS
  Antioxidant activity of the methanol extract and its derived fractions petroleum ether (APP), carbon tetrachloride (APC), dichloromethane (APD), ethyl acetate (APE), and residual aqueous fraction (APA) of the leaves of Albizia procera was performed by in vitro chemical analyses. Total phenolic content of the APM and other five fractions were also determined. APM and its derived fractions were also subjected to preliminary phytochemical screening test for various constituents.
  
  
  RESULTS
  Phytochemical screening revealed the presence of saponins, steroids, tannins, glycosides and flavonoids in the extracts. Amongst the extracts, APE showed the highest total phenolic content (449.18 ± 18.41mg of gallic acid equivalent/g of extract). In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging test, the IC(50) value of APM, APP, APC, APD, APE and APA was 43.43, 63.60, 166.18, 41.15, 11.79, and 63.06 μg/mL, respectively. Therefore, among the APM and its derived fractions, APE showed the highest antioxidant activity which is comparable to that of standard ascorbic acid (AA) (IC(50) 10.12 μg/mL). The total antioxidant capacity was found to be varied in different fractions. The reducing activity on ferrous ion was ranked as APE > APD > APM > APA > APC.
  
  
  CONCLUSIONS
  The above evidences suggest that APE of A. procera leaf is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: Mahfuza
authors.initials: M
authors.lastname: khatoon
authors.forename: Ekramul
authors.initials: E
authors.lastname: Islam
authors.forename: Rafikul
authors.initials: R
authors.lastname: Islam
authors.forename: Aziz Abdur
authors.initials: AA
authors.lastname: Rahman
authors.forename: A H M Khurshid
authors.initials: AH
authors.lastname: Alam
authors.forename: Proma
authors.initials: P
authors.lastname: Khondkar
authors.forename: Mamunur
authors.initials: M
authors.lastname: Rashid
authors.forename: Shahnaj
authors.initials: S
authors.lastname: Parvin
mesh_terms: D000085:Acetates; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals; D000432:Methanol; D019800:Phenol / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012997:Solvents
chemical_list: D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D019800:Phenol; C007650:ethyl acetate; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol