title: Evaluation of osteogenic cell culture and osteogenic/peripheral blood mononuclear human cell co-culture on modified titanium surfaces.
abstract: This study aimed to determine the effect of a bioactive ceramic coating on titanium in the nanothickness range on human osteogenic cells, peripheral blood mononuclear cells (PBMC) and on osteogenic cells co-cultured with PBMC without exogenous stimuli. Cell viability, proliferation, adhesion, cytokine release (IL1β, TGFβ1, IL10 and IL17) and intracellular stain for osteopontin and alkaline phosphatase were assessed. Morphologic evaluation showed smaller and less spread cell aspects in co-culture relative to osteogenic cell culture. Cell viability, proliferation and adhesion kinetics were differently influenced by surface texture/chemistry in culture versus co-culture. Cytokine release was also influenced by the interaction between mononuclear and osteogenic cells (mediators released by mononuclear cells acted on osteogenic cells and vice versa). In general, 'multi-cell type' interactions played a more remarkable role than the surface roughness or chemistry utilized on the in vitro cellular events related to initial stages of bone formation.
authors.affiliation: Department of Cell Biology, Universidade Federal do Triângulo Mineiro, Uberaba, Brazil.
authors.forename: Camilla G
authors.initials: CG
authors.lastname: Moura
authors.forename: Maria A
authors.initials: MA
authors.lastname: Souza
authors.forename: Ralf J
authors.initials: RJ
authors.lastname: Kohal
authors.forename: Paula
authors.initials: P
authors.lastname: Dechichi
authors.forename: Darceny
authors.initials: D
authors.lastname: Zanetta-Barbosa
authors.forename: Ryo
authors.initials: R
authors.lastname: Jimbo
authors.forename: Cristina C
authors.initials: CC
authors.lastname: Teixeira
authors.forename: Hellen S
authors.initials: HS
authors.lastname: Teixeira
authors.forename: Nick
authors.initials: N
authors.lastname: Tovar
authors.forename: Paulo G
authors.initials: PG
authors.lastname: Coelho
mesh_terms: D002448:Cell Adhesion; D002470:Cell Survival; D002516:Ceramics / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D010012:Osteogenesis; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D016207:Cytokines; D014025:Titanium