title: Efficacy of decoquinate against Sarcocystis neurona in cell cultures.
abstract: Decoquinate is a quinolone anticoccidial approved for use in the prevention of intestinal coccidiosis in farm animals. This compound has good activity against Toxoplasma gondii and Neospora caninum in cell cultures. The drug acts on the parasites' mitochondria. The activity of decoquinate against developing merozoites of 2 isolates of Sarcocystis neurona was examined in cell culture. Merozoite production at 10 days was completely inhibited when decoquinate was used at 20 or 240 nM. The IC50 of decoquinate was 0.5 ± 0.09nM for the Sn6 isolate of S. neurona from a horse and 1.1 ± 0.6 nM for the SnOP15 isolate of S. neurona from an opossum. Levamisole was toxic at 5 μg/ml and no synergism was observed when decoquinate was combined with levamisole and tested against the Sn3YFP isolate of S. neurona. Decoquinate was cidal for developing schizonts of S. neurona at 240 nM.
authors.affiliation: Department of Biomedical Science and Pathology, Virginia Tech, 1401 Prices Fork Road, Blacksburg, VA 24061, USA. lindsayd@vt.edu
authors.forename: David S
authors.initials: DS
authors.lastname: Lindsay
authors.forename: M Mudasser
authors.initials: MM
authors.lastname: Nazir
authors.forename: Azhar
authors.initials: A
authors.lastname: Maqbool
authors.forename: Siobhan P
authors.initials: SP
authors.lastname: Ellison
authors.forename: Jeannine S
authors.initials: JS
authors.lastname: Strobl
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003049:Coccidiostats / Q000494:pharmacology; D003667:Decoquinate / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000166:cytology; D007978:Levamisole / Q000494:pharmacology; D012522:Sarcocystis / Q000187:drug effects*
chemical_list: D000969:Antinematodal Agents; D003049:Coccidiostats; D007978:Levamisole; D003667:Decoquinate
keywords: Decoquinate; Levamisole; Sarcocystis neurona