title: Molecular characterization of clinical isolates of herpes simplex virus type 1 collected in a tertiary-care hospital in Dublin, Ireland.
abstract: Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen. While there has been extensive research into the evolutionary relationships among herpesviruses, there is little data on the evolutionary relationship of HSV-1 based on sequence analysis of clinical isolates. The present study aims to be the first to document the molecular epidemiology and genetic diversity and frequency of recombination of HSV-1 (n = 42) clinical isolates in Ireland. The entire 1,171 bp of the gI-1 gene and 717 bp of the gG-1 gene of 42 clinical Irish isolates were amplified, sequenced and the phylogenies reconstructed. Putative recombinants were examined using bootscan analysis. Phylogenetic reconstruction of the nucleotide sequence alignments of the entire genes of amplified glycoproteins gI and gG suggested that three distinct HSV-1 genogroups were circulating in the Irish population. At least 15 HSV-1 intergenic recombinants with a recombination point between gI and gG, and 11 HSV-1 intragenic recombinants were detected. There was no evident association between genetic group and gender, disease recurrence or anatomical site of infection. Genital isolates (n = 30) belonged to all genogroups. However, two HSV-1 isolates, Irl 31 and Irl32, from a patient with severe mucocutaneous infection nonresponsive to acyclovir and isolated over a prolonged period were both intragenic and intergenic recombinants. The detection of variability and recombination in gG and gI genes of both HSV-1 may provide a mechanism to evade the host immune response thereby maintaining the viral genome. The variability and recombination detected may also have implications for the detection, diagnosis and treatment of HSV.
authors.affiliation: Department of Microbiology, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Lisa
authors.initials: L
authors.lastname: Rose
authors.forename: Brendan
authors.initials: B
authors.lastname: Crowley
mesh_terms: D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006561:Herpes Simplex / Q000453:epidemiology / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007494:Ireland / Q000453:epidemiology; D017720:Molecular Epidemiology; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D062606:Tertiary Care Centers; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C064959:glycoprotein I, herpes simplex virus type 1; C050591:glycoprotein gG-1, herpes simplex virus type 1