title: Distribution of hydrocarbon-degrading bacteria in the soil environment and their contribution to bioremediation.
abstract: A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed throughout the soil environment and ranged from 3.7 × 10(7) to 5.0 × 10(8) cells/g-soil, and the ratio to total bacteria was 0.1-4.3 %. The dynamics of total bacteria, indigenous HDB, and Rhodococcus erythropolis NDKK6 (carrying alkB R2) during bioremediation were analyzed. During bioremediation with an inorganic nutrient treatment, the numbers of these bacteria were slightly increased. The numbers of HDB (both indigenous bacteria and strain NDKK6) were gradually decreased from the middle stage of bioremediation. Meanwhile, the numbers of these bacteria were highly increased and were maintained during bioremediation with an organic nutrient. The organic treatment led to activation of not only the soil bacteria but also the HDB, so an efficient bioremediation was carried out.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Life Science, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fukuhara
authors.forename: Sachie
authors.initials: S
authors.lastname: Horii
authors.forename: Toshihide
authors.initials: T
authors.lastname: Matsuno
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Matsumiya
authors.forename: Masaki
authors.initials: M
authors.lastname: Mukai
authors.forename: Motoki
authors.initials: M
authors.lastname: Kubo
mesh_terms: D000473:Alkanes / Q000378:metabolism; D058491:Bacterial Load; D001673:Biodegradation, Environmental; D058448:Biota; D002244:Carbon / Q000378:metabolism; D006838:Hydrocarbons / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism; D012240:Rhodococcus / Q000235:genetics / Q000378:metabolism*; D012987:Soil; D012988:Soil Microbiology; D013997:Time Factors
chemical_list: D000473:Alkanes; D006838:Hydrocarbons; D010578:Petroleum; D012987:Soil; D002244:Carbon; D006899:Mixed Function Oxygenases