title: [TSP-2 suppresses the expression and DNA-binding activity of nuclear factor-κB p65 protein in mice with ulcerative colitis].
abstract: OBJECTIVE
  To observe the effect of TSP-2, the antibody of Toll-like receptor 2 extracellular domain, on the expression and DNA-binding activity of nuclear factor-κB (NF-κB) p65 protein in mice with ulcerative colitis (UC).
  
  
  METHODS
  Sixty BALB/c mice were randomized equally into normal control group, UC model group, TSP-2 treatment group, and rabbit IgG treatment group. In the latter 3 groups, the mice were fed with 5% DSS (C6H7Na3O14S3) solution for 7 days to induced UC, followed then by treatment with daily injections of TSP-2 or rabbit IgG as appropriate for 7 days. The disease activity index was recorded during the treatment. The colitis tissues were collected after the treatments for HE staining and detecting the expression and DNA-binding activity of NF-κB p65 in the colon mucosa by Western blotting and ELISA.
  
  
  RESULTS
  The DNA binding activity and expressions of NF-κB P65 protein increased significantly in UC model group (P<0.05). TSP-2 treatment group significantly decreased the disease activity index (P<0.05) and lowered the DNA-binding activity and expression of NF-κB P65 protein (P<0.05) in the UC mouse models, while rabbit IgG produced no such effects (P>0.05).
  
  
  CONCLUSIONS
  TSP-2 can suppress the DNA-binding activity and protein expressions of NF-κB P65 and regulate excessive immune response in the intestines to ameliorate ulcerative colitis in mice.
authors.affiliation: Department of Gastroenterology, Shenzhen Municipal People's Hospital/ Second Clinical Medical College of Jinan University, Shenzhen 518020, China. laimingguang@yahoo.com.cn
authors.forename: Mingguang
authors.initials: M
authors.lastname: Lai
authors.forename: Lisheng
authors.initials: L
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Chen
authors.initials: C
authors.lastname: Wei
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D019699:Thrombospondins / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*; D000099262:Thrombospondin 2
chemical_list: D019699:Thrombospondins; D051996:Transcription Factor RelA; D000099262:Thrombospondin 2; C498144:Rela protein, mouse