title: An immunoassay utilizing the DNA-coated polydiacetylene micelles as a signal generator.
abstract: Immunoassay is an important technique to detect the disease biomarkers and pathogenic biological agents which often present at low levels in clinical samples. To improve sensitivity of the immunoassay, here we described the DNA-coated, nano-sized micelles in which the DNA strands play a role as signal generators in an immunoassay. This micelle-based immunoassay was evaluated for quantitation of a liver cancer biomarker and the sensitivity of the method was compared with those of the conventional methods.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil, Seongbuk-gu, Seoul 136 791, Republic of Korea.
authors.forename: Hoa Thi
authors.initials: HT
authors.lastname: Hoang
authors.forename: Taemin
authors.initials: T
authors.lastname: Lee
authors.forename: Byeong-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Ki-Cheol
authors.initials: KC
authors.lastname: Han
authors.forename: Dae-Ro
authors.initials: DR
authors.lastname: Ahn
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism; D008823:Micelles; D049329:Nanostructures / Q000737:chemistry; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D015347:RNA Probes / Q000737:chemistry / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D008823:Micelles; D000078789:Polyacetylene Polymer; D011108:Polymers; D015347:RNA Probes; D053279:Polyynes; C082361:polydiacetylene; D004247:DNA; D016914:Ribonuclease H