title: A versatile star PEG grafting method for the generation of nonfouling and nonthrombogenic surfaces.
abstract: Polyethylene glycol (PEG) grafting has a great potential to create nonfouling and nonthrombogenic surfaces, but present techniques lack versatility and stability. The present work aimed to develop a versatile PEG grafting method applicable to most biomaterial surfaces, by taking advantage of novel primary amine-rich plasma-polymerized coatings. Star-shaped PEG covalent binding was studied using static contact angle, X-ray photoelectron spectroscopy (XPS), and quartz crystal microbalance with dissipation monitoring (QCM-D). Fluorescence and QCM-D both confirmed strong reduction of protein adsorption when compared to plasma-polymerized coatings and pristine poly(ethyleneterephthalate) (PET). Moreover, almost no platelet adhesion was observed after 15 min perfusion in whole blood. Altogether, our results suggest that primary amine-rich plasma-polymerized coatings offer a promising stable and versatile method for PEG grafting in order to create nonfouling and nonthrombogenic surfaces and micropatterns.
authors.affiliation: Laboratory of Endovascular Biomaterials (LBeV), Research Centre, Centre Hospitalier de l'Université de Montreal (CRCHUM), 2099 Alexandre de Sève, Montreal, QC, Canada H2L 2W5.
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Thalla
authors.forename: Angel
authors.initials: A
authors.lastname: Contreras-García
authors.forename: Hicham
authors.initials: H
authors.lastname: Fadlallah
authors.forename: Jérémie
authors.initials: J
authors.lastname: Barrette
authors.forename: Gregory
authors.initials: G
authors.lastname: De Crescenzo
authors.forename: Yahye
authors.initials: Y
authors.lastname: Merhi
authors.forename: Sophie
authors.initials: S
authors.lastname: Lerouge
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000187:drug effects; D002318:Cardiovascular Diseases / Q000628:therapy; D004867:Equipment Design; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D010973:Platelet Adhesiveness; D011092:Polyethylene Glycols / Q000737:chemistry*; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates; D011108:Polymers; D011506:Proteins; D014867:Water; D011092:Polyethylene Glycols