title: Flow field-flow fractionation: critical overview.
abstract: This overview regards some critical issues in performing flow field-flow fractionation (flow FFF, FlFFF, AF4, HF-FlFFF, HF5). It includes the channel thickness, void time, channel-flow parabolic profile, channel-flow velocity gradient, uniformity of the cross-flow, sample injection time, relaxation/focusing time, width of sample starting zone, retention level, theoretical and experimental zone broadening, hydrodynamic threshold immobilisation/re-mobilisation, sample loss and adsorption, membrane fouling, sample mass overloading, problems with symmetrical channels, non-spherical sample particles, and method development. Good method development practice (GMDP) and good fractogram practice (GFP) is suggested.
authors.affiliation: Unit for Analysis and Synthesis, Department of Chemistry, Center for Chemistry and Chemical Engineering, Faculty of Engineering LTH, Lund University, Box 124, SE-221 00 Lund, Sweden. Karl-Gustav.Wahlund@analykem.lu.se
authors.forename: Karl-Gustav
authors.initials: KG
authors.lastname: Wahlund
mesh_terms: D031521:Fractionation, Field Flow / Q000379:methods*; D008567:Membranes, Artificial; D011506:Proteins / Q000302:isolation & purification; D014462:Ultrafiltration
chemical_list: D008567:Membranes, Artificial; D011506:Proteins