title: Protein interaction discovery using parallel analysis of translated ORFs (PLATO).
abstract: Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of LYN kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib.
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.|Howard Hughes Medical Institute.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA.|Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.|Howard Hughes Medical Institute.
authors.forename: H Benjamin
authors.initials: HB
authors.lastname: Larman
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.|Howard Hughes Medical Institute.
authors.forename: Geng
authors.initials: G
authors.lastname: Gao
authors.affiliation: Human Oncology and Pathogenesis Program, Memorial Sloan-Kettering Cancer Center, New York, NY.
authors.forename: Romel
authors.initials: R
authors.lastname: Somwar
authors.affiliation: Department of Chemistry, University of Massachusetts Boston, Boston, MA.
authors.forename: Zijuan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.|Department of Mathematics, Massachusetts Institute of Technology, Cambridge, MA.
authors.forename: Uri
authors.initials: U
authors.lastname: Laserson
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.|Howard Hughes Medical Institute.
authors.forename: Alberto
authors.initials: A
authors.lastname: Ciccia
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.|Howard Hughes Medical Institute.
authors.forename: Natalya
authors.initials: N
authors.lastname: Pavlova
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.
authors.forename: George
authors.initials: G
authors.lastname: Church
authors.affiliation: Department of Chemistry, University of Massachusetts Boston, Boston, MA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Division of Neuro-Oncology, Translational Neuro-Oncology Laboratories, Department of Neurosciences, U.C. San Diego, Moores Cancer Center, La Jolla, CA.
authors.forename: Santosh
authors.initials: S
authors.lastname: Kesari
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA.|Department of Genetics, Harvard University Medical School, Boston, MA.|Howard Hughes Medical Institute.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Elledge
mesh_terms: D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics*; D011485:Protein Binding; D014176:Protein Biosynthesis; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases