title: Analysis of genetic variability and population structure of the endemic medicinal Limonium sinense using molecular markers.
abstract: Limonium sinense is an endemic medicinal herb used to treat fever, hemorrhage and other disorders. In the present study, population genetic diversity was elucidated using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) primers. Percentage of polymorphic bands, Nei's gene diversity and Shannon's information index revealed a high level of genetic diversity at species level. The analysis of molecular variance revealed that 69.88% (RAPD), 71.19% (ISSR) and 70.97% (AFLP) of variability were partitioned among individuals within populations, which indicated the coherent trend by Gst (0.3849/0.3577/0.3670). Gene flow number (Nm) was 0.581/0.618/0.612, which indicated that there was a limited gene exchange between populations. The UPGMA clustering results showed that the genetic distance had no significant correlation with geographic distance. These results indicate that these markers were reliable tools for the differentiation and determination of the genetic diversity among the populations of L. sinense and the conservation of existing natural population is necessary.
authors.affiliation: Chemical and Biological Engineering College, Yancheng Institute of Technology, Jiangsu, Yancheng 224003, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Ding
authors.forename: Daizhen
authors.initials: D
authors.lastname: Zhang
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Yu
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhao
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002681:China; D051456:Gene Flow; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005843:Geography; D018895:Microsatellite Repeats / Q000235:genetics; D010802:Phylogeny; D029606:Plumbaginaceae / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
chemical_list: D005819:Genetic Markers