title: Alleviation of chromium toxicity in rice seedlings by applying exogenous glutathione.
abstract: The effect of exogenous reduced glutathione (GSH) on alleviation of hexavalent chromium (Cr(6+)) toxicity to rice seedlings and its physiological mechanisms were comprehensively investigated in a series of experiments. Our results showed that growth and nutrient uptake of rice seedlings were dramatically reduced under 100 μM Cr(6+) stress, and the reduction was significantly alleviated by exogenous GSH. Cr(6+) stress also reduced cell viability in root tips and damaged ultrastructure of both chloroplasts and root cells, while the addition of GSH alleviates those negative effects. Cr-induced toxicity and GSH-caused Cr alleviation differed significantly between Cr-tolerant Line 117 (L117) and Cr-sensitive Line 41 (L41). Under Cr(6+) stress, cystine content was increased and GSH content was decreased in rice plants, exogenous GSH, however, mitigated the Cr-toxicity by reversing the Cr-induced changes of the two compounds. The types of Cr-induced secretion of organic acids varied between the genotypes, where reduction in the contents of acetic and lactic acids and tartaric and malic acids were observed in L117 and L41, respectively. The addition of GSH alleviated the reduction of secretion of these organic acids. Exogenous GSH also altered the forms of Cr ions in the rhizosphere and the fraction of distribution at subcellular level in both shoots and roots. It may be concluded that the alleviation of Cr(6+) toxicity by exogenous GSH is directly attributed to its regulation on forms of Cr ions in rhizosphere and their distribution at subcellular levels.
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
authors.forename: Boyin
authors.initials: B
authors.lastname: Qiu
authors.forename: Fanrong
authors.initials: F
authors.lastname: Zeng
authors.forename: Shengguan
authors.initials: S
authors.lastname: Cai
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Wu
authors.forename: Shamsi Imran
authors.initials: SI
authors.lastname: Haider
authors.forename: Feibo
authors.initials: F
authors.lastname: Wu
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D002264:Carboxylic Acids / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002857:Chromium / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity*; D005978:Glutathione / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D018517:Plant Roots / Q000502:physiology; D036226:Seedlings / Q000187:drug effects
chemical_list: D002264:Carboxylic Acids; D002857:Chromium; D005978:Glutathione