title: Next-generation sequencing-based transcriptome profiling analysis of Pohlia nutans reveals insight into the stress-relevant genes in Antarctic moss.
abstract: Genome-wide characterization of the Pohlia nutans transcriptome is essential for clarifying the role of stress-relevant genes in Antarctic moss adapting to the extreme polar environment. High-throughput Illumina sequencing was used to analyze the gene expression profile of P. nutans after cold treatment. A total of 93,488 unigenes, with an average length of 405 bp, were obtained. Gene annotation showed that 16,781 unigenes had significant similarity to known functional protein-coding genes, most of which were annotated using the GO, KOG and KEGG pathway databases. Global profiling of the differentially expressed genes revealed that 3,796 unigenes were significantly upregulated after cold treatment, while 1,405 unigenes were significantly downregulated. In addition, 816 receptor-like kinases and 1,309 transcription factors were identified from P. nutans. This overall survey of transcripts and stress-relevant genes can contribute to understanding the stress-resistance mechanism of Antarctic moss and will accelerate the practical exploitation of the genetic resources for this organism.
authors.affiliation: Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xian-Xia Ling Road, High-tech Park, Qingdao, 266061 Shandong, People's Republic of China.
authors.forename: Shenghao
authors.initials: S
authors.lastname: Liu
authors.forename: Nengfei
authors.initials: N
authors.lastname: Wang
authors.forename: Pengying
authors.initials: P
authors.lastname: Zhang
authors.forename: Bailin
authors.initials: B
authors.lastname: Cong
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Lin
authors.forename: Shouqiang
authors.initials: S
authors.lastname: Wang
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Huang
mesh_terms: D019068:Bryopsida / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D013312:Stress, Physiological / Q000235:genetics*; D059467:Transcriptome