title: [Influence of cryopreservation and lyophilization on exopolysaccharide synthesis and viability of Xanthomonas campestris pv. campestris IMV B-7001].
abstract: It is shown, that the producer of exopolysaccharides Xantomonas campestris pv. campestris IMV B-7001 is more sensitive to freeze-drying, than to freezing-thawing and keeps stable morphological properties. Predicted preservation of viability is within the limits of 2-8 years at -70 degrees C and exceeds 30 years at -135 degrees C and freeze-drying. Results of the latter essentially depend on the medium composition and storage conditions. Synthesis of exopolysaccharides at preservation is kept, showing the tendency to temporal increase of productivity at regeneration from anabiosis. Such stimulating effect is more expressed at cryopreservation and depends on the cryoprotector and freezing temperature. The investigated cryoprotectors are arranged in a line according to their efficiency: 10% sucrose +1% gelatin > or = 10% glycerin > 5% dimethylsulfoxide > 4% ethylene glycol.
authors.forename: T N
authors.initials: TN
authors.lastname: Golovach
authors.forename: L I
authors.initials: LI
authors.lastname: Groma
mesh_terms: D003080:Cold Temperature; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D005612:Freeze Drying / Q000379:methods*; D005615:Freezing; D005780:Gelatin / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013395:Sucrose / Q000494:pharmacology; D016959:Xanthomonas campestris / Q000187:drug effects / Q000502:physiology*
chemical_list: D003451:Cryoprotective Agents; D011135:Polysaccharides, Bacterial; D013395:Sucrose; D005780:Gelatin; D019855:Ethylene Glycol; D005990:Glycerol; D004121:Dimethyl Sulfoxide