title: Molecular characterisation of the amino- and carboxyl-domains in different Glu-A1x alleles of Triticum urartu Thum. ex Gandil.
abstract: The wild diploid wheat (Triticum urartu Thum. ex Gandil.) is a potential gene source for wheat breeding, as this species has been identified as the A-genome donor in polyploid wheats. One important wheat breeding trait is bread-making quality, which is associated in bread wheat (T. aestivum ssp. aestivum L. em. Thell.) with the high-molecular-weight glutenin subunits. In T. urartu, these proteins are encoded by the Glu-A1x and Glu-A1Ay genes at the Glu-A (u) 1 locus. The Glu-A1x genes of 12 Glu-A (u) 1 allelic variants previously detected in this species were analysed using PCR amplification and sequencing. Data showed wide diversity for the Glu-A1x alleles in T. urartu, which also showed clear differences to the bread wheat alleles. This variation could enlarge the high-quality genetic pool of modern wheat and be used to diversify the bread-making quality in durum (T. turgidum ssp. durum Desf. em. Husn.) and common wheat.
authors.affiliation: Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Universidad de Córdoba, Edificio Gregor Mendel, Campus de Rabanales, CeiA3, 14071 Córdoba, Spain. jb.alvarez@uco.es
authors.forename: Juan B
authors.initials: JB
authors.lastname: Alvarez
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Gutiérrez
authors.forename: Carlos
authors.initials: C
authors.lastname: Guzmán
authors.forename: Luis M
authors.initials: LM
authors.lastname: Martín
mesh_terms: D000483:Alleles; D018745:Genome, Plant; D005983:Glutens / Q000235:genetics*; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014908:Triticum / Q000235:genetics*
chemical_list: D021122:Protein Subunits; D005983:Glutens; C037302:glutenin