title: Cloud-based solution to identify statistically significant MS peaks differentiating sample categories.
abstract: BACKGROUND
  Mass spectrometry (MS) has evolved to become the primary high throughput tool for proteomics based biomarker discovery. Until now, multiple challenges in protein MS data analysis remain: large-scale and complex data set management; MS peak identification, indexing; and high dimensional peak differential analysis with the concurrent statistical tests based false discovery rate (FDR). "Turnkey" solutions are needed for biomarker investigations to rapidly process MS data sets to identify statistically significant peaks for subsequent validation.
  
  
  RESULTS
  Here we present an efficient and effective solution, which provides experimental biologists easy access to "cloud" computing capabilities to analyze MS data. The web portal can be accessed at http://transmed.stanford.edu/ssa/.
  
  
  CONCLUSIONS
  Presented web application supplies large scale MS data online uploading and analysis with a simple user interface. This bioinformatic tool will facilitate the discovery of the potential protein biomarkers using MS.
authors.affiliation: Departments of Surgery, Stanford University, Stanford, CA 94305, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Ling
authors.forename: Helen
authors.initials: H
authors.lastname: Jiang
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Wen
authors.forename: John C
authors.initials: JC
authors.lastname: Whitin
authors.forename: Lu
authors.initials: L
authors.lastname: Tian
authors.forename: Harvey J
authors.initials: HJ
authors.lastname: Cohen
authors.forename: Xuefeng B
authors.initials: XB
authors.lastname: Ling
mesh_terms: D000465:Algorithms; D019540:Area Under Curve; D015415:Biomarkers / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D003627:Data Interpretation, Statistical; D006801:Humans; D020407:Internet; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013223:Statistics as Topic
chemical_list: D015415:Biomarkers; D020543:Proteome