title: Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library.
abstract: RNA interference (RNAi) is an endogenous RNA-destruction phenomenon induced by certain double-stranded RNAs (dsRNAs). In RNAi, dsRNAs are processed into small interfering RNAs (siRNAs) which in turn trigger the cleavage of the target mRNA. Here, using a short hairpin RNA-expression library, we identified a DEAD-box helicase 3, DDX3, as an essential factor involved in RNAi pathway and revealed that DDX3 is colocalized with Ago2, an essential factor in RNAi pathway that cleaves target mRNA. Results of experiments with a dominant negative mutant of DDX3 further confirmed that this factor affects the RNAi activity. Together, DDX3 functions to assure mammalian RNAi pathway. Together, our results indicate that DDX3 is a new key molecule to understand the molecular mechanism underlying RNAi pathway in mammals.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.
authors.forename: Vivi
authors.initials: V
authors.lastname: Kasim
authors.forename: Shourong
authors.initials: S
authors.lastname: Wu
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyagishi
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism*; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008156:Luciferases; D016297:Mutagenesis, Site-Directed; D034622:RNA Interference / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D008156:Luciferases; C119332:DDX3X protein, human; D053487:DEAD-box RNA Helicases