title: MiR-449c targets c-Myc and inhibits NSCLC cell progression.
abstract: MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in this study. NSCLC cell proliferation and invasion assays indicate that transfection of miR-449c expression plasmid inhibits the proliferation and invasion ability of NCI-H23 and NCI-H838 cells. In addition, miR-449c overexpression could suppress tumor growth in vivo. Morever, c-Myc was identified as a direct target gene of miR-449c. These findings clearly suggest that miR-449c downregulation and c-Myc amplification may be involved in the development of NSCLC.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Open Laboratory of Clinical Medicine and Key Subject, Henan Institution of Higher Education, PR China. miaolily@126.com
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Miao
authors.forename: Shi-Fu
authors.initials: SF
authors.lastname: Huang
authors.forename: Zhen-Tao
authors.initials: ZT
authors.lastname: Sun
authors.forename: Zeng-Yan
authors.initials: ZY
authors.lastname: Gao
authors.forename: Rui-Xia
authors.initials: RX
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*
chemical_list: C544530:MIRN449 microRNA, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc