title: Analysis of peptidome profiling of serum from patients with early onset symptoms of ischemic stroke.
abstract: BACKGROUND
  We sought to identify new serum biomarkers for the early diagnosis of ischemic stroke.
  
  
  METHODS
  We collected 63 serum samples from patients with neurologic disease (45 patients with ischemic stroke, 18 patients with other neurologic disorders, and 56 healthy controls). Serum peptides were extracted using immobilized copper ion chromatography on a robotic platform. Mass spectra were acquired by matrix-assisted laser desorption/ionization-time of flight mass spectrometry using an Autoflex II spectrometer (Bruker Daltonics, Billerica, MA). Statistical analyses were performed with Clinprotools 2.2 software (Bruker Daltonics) and SPSS software (version 15.0; SPSS, Inc., Chicago, IL).
  
  
  RESULTS
  No peptide biomarker or panel of peptide biomarkers was identified to differentiate between ischemic stroke and other neurologic disease, but ischemic stroke patients were differentiated from healthy controls with a single feature of the peptidome (sensitivity 88.6%; specificity 96.4%).
  
  
  CONCLUSIONS
  Analysis of peptidome profiling of serum could be a useful tool in the search for early diagnostic biomarkers of ischemic stroke.
authors.affiliation: Department of Biochemistry, Hospital Clínic, Barcelona, Spain. Electronic address: nrico@clinic.ub.es.
authors.forename: Naira
authors.initials: N
authors.lastname: Rico Santana
authors.affiliation: Department of Biochemistry, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Edgar
authors.initials: E
authors.lastname: Zapico Muñiz
authors.affiliation: Department of Neurology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Cocho
authors.affiliation: Department of Neurology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Bravo
authors.affiliation: Department of Neurology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Delgado Mederos
authors.affiliation: Department of Neurology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Martí-Fàbregas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D002545:Brain Ischemia / Q000097:blood* / Q000175:diagnosis; D016022:Case-Control Studies; D002852:Chromatography, Ion Exchange; D003937:Diagnosis, Differential; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D040901:Proteomics / Q000379:methods; D012371:Robotics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020521:Stroke / Q000097:blood* / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: MALDI; biomarker; serum peptide profiling; stroke