title: Development of polymeric nanoprobes with improved lifetime dynamic range and stability for intracellular oxygen sensing.
abstract: A class of core-shell nanoparticles possessing a layer of biocompatible shell and hydrophobic core with embedded oxygen-sensitive platinum-porphyrin (PtTFPP) dyes is developed via a radical-initiated microemulsion co-polymerization strategy. The influences of host matrices and the PtTFPP incorporation manner on the photophysical properties and the oxygen-sensing performance of the nanoparticles are investigated. Self-loading capability with cells and intracellular-oxygen-sensing ability of the as-prepared nanoparticle probes in the range 0%-20% oxygen concentration are confirmed. Polymeric nanoparticles with optimized formats are characterized by their relatively small diameter (<50 nm), core-shell structures with biocompatible shells, covalent-attachment-imparted leak-free construction, improved lifetime dynamic range (up to 44 μs), excellent storage stability and photostability, and facile cell uptake. The nanoparticles' small sensor diameter and core-shell structure with biocompatible shell make them suitable for intracellular detection applications. For intracellular detection applications, the leak-free feature of the as-prepared nanoparticle sensor effectively minimizes potential chemical interferences and cytotoxicity. As a salient feature, improved lifetime dynamic range of the sensor is expected to enable precise oxygen detection and control in specific practical applications in stem-cell biology and medical research. Such a feature-packed nanoparticle oxygen sensor may find applications in precise oxygen-level mapping of living cells and tissue.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences-UCAS, Beijing 100049, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Xian
authors.initials: X
authors.lastname: Hao
authors.forename: Haijiao
authors.initials: H
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
authors.forename: Debao
authors.initials: D
authors.lastname: Xiao
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Tian
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002522:Chlorocebus aethiops; D042541:Intracellular Space / Q000737:chemistry*; D049449:Luminescence; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D010100:Oxygen / Q000032:analysis*; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D020058:Styrene / Q000737:chemistry; D014709:Vero Cells
chemical_list: D011108:Polymers; D011166:Porphyrins; D020058:Styrene; D010984:Platinum; D010100:Oxygen
keywords: core/shell nanoparticles; nanomaterials; oxygen-level mapping; photoluminescence; polymeric nanoprobes