title: SCARB1 single nucleotide polymorphism (rs5888) is associated with serum lipid profile and myocardial infarction in an age- and gender-dependent manner.
abstract: BACKGROUND
  Mutation in SCARB1 gene, exon 8 rs5888, has been associated with altered lipid levels and cardiovascular risk in humans though the results have been inconsistent. We analysed the impact of SCARB1 single nucleotide polymorphism (SNP) rs5888 with plasma lipid profile and association with coronary artery disease (CAD) in a Lithuanian population characterized by high morbidity and mortality from CAD and high prevalence of hypercholesterolemia.
  
  
  METHODS
  The study included 1976 subjects from a random sample (reference group) and an myocardial infarction (MI) group of 463 patients. Genotyping of SCARB1 (rs5888) was carried out using the real-time polymerase chain reaction method.
  Analysis of rs5888 C/T gene polymorphism in the reference group revealed that male TT genotype carriers (25-74 years) had significantly higher total cholesterol and triglyceride concentrations (5.70 mmol/l vs. 5.49 mmol/l; p = 0.036, and 1.70 mmol/l vs. 1.40 mmol/l, p = 0.023, respectively) than CT carriers and the oldest males (65-74 years) TT carriers had significantly higher high density lipoprotein cholesterol concentrations in comparison to heterozygous (1.52 mmol/l vs. 1.36 mmol/l, p = 0.033). The youngest female (25-44 years) TT genotype carriers had significantly lower low density lipoprotein cholesterol concentrations in comparison to C homozygous (2.59 mmol/l vs. 2.92 mmol/l, p = 0.023). The frequency of the SCARB1 TT genotype in the oldest male MI group (65-74 years) was significantly lower than in the corresponding reference group subjects (9.4% vs. 22.3%, p = 0.006). SCARB1 TT genotype was associated with decreased odds of MI in males aged 65-75 years (OR = 0.24, 95% CI 0.10-0.56, p = 0.001).
  
  
  CONCLUSIONS
  SCARB1 polymorphism is associated with lipid metabolism and CAD in an age- and gender- dependent manner. Analysis of SCARB1 SNP rs5888 C/T genotypes revealed an atheroprotective phenotype of lipid profile in older men and in young women TT genotype carriers in the reference group. SCARB1 TT genotype was associated with decreased odds of MI in aged men.
authors.affiliation: Department of Ophthalmology, Medicine Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania. daivastanislovaitiene@yahoo.com
authors.forename: Daiva
authors.initials: D
authors.lastname: Stanislovaitiene
authors.forename: Vaiva
authors.initials: V
authors.lastname: Lesauskaite
authors.forename: Dalia
authors.initials: D
authors.lastname: Zaliuniene
authors.forename: Alina
authors.initials: A
authors.lastname: Smalinskiene
authors.forename: Olivija
authors.initials: O
authors.lastname: Gustiene
authors.forename: Diana
authors.initials: D
authors.lastname: Zaliaduonyte-Peksiene
authors.forename: Abdonas
authors.initials: A
authors.lastname: Tamosiunas
authors.forename: Dalia
authors.initials: D
authors.lastname: Luksiene
authors.forename: Janina
authors.initials: J
authors.lastname: Petkeviciene
authors.forename: Remigijus
authors.initials: R
authors.lastname: Zaliunas
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D005091:Exons; D005260:Female; D060005:Genotyping Techniques; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D051122:Scavenger Receptors, Class B / Q000235:genetics*; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; D014280:Triglycerides