title: Mastoid obliteration using three-dimensional composite scaffolds consisting of polycaprolactone/β-tricalcium phosphate/collagen nanofibers: an in vitro and in vivo study.
abstract: Two different composite scaffolds, solid-freeform-fabricated PCL/β-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular activities of osteoblast-like-cells (MG63), SEM images and MTT assays are conducted. Experimental mastoid obliteration is performed using guinea pigs that are divided group A (PCL/β-TCP/collagen-nanofiber scaffold) and group B (PCL/β-TCP scaffold). The results reveal that PCL/β-TCP/collagen scaffold provide much broader cell attachment sites than PCL/β-TCP scaffold. The µ-CT and fluorescent microscopy results reveal that the acceleration of early new bone formation within the pores and scaffold itself at week 4 post-operation is more effective in group A. In addition, based on the results of the histological and µ-CT at 12 weeks post-surgery, the effective regeneration of bone in the PCL/β-TCP/collagen scaffold is appeared.
authors.affiliation: Department of Otolaryngology, Chonnam National University Medical School, Gwangju; Research Center for Resistant Cells, Chosun Medical School, Gwangju. chulsavio@hanmail.net
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Jang
authors.forename: Yong Beom
authors.initials: YB
authors.lastname: Cho
authors.forename: Myung Gu
authors.initials: MG
authors.lastname: Yeo
authors.forename: Geun Hyung
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D055119:Elastic Modulus / Q000187:drug effects; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008416:Mastoid / Q000000981:diagnostic imaging / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water; D055114:X-Ray Microtomography
chemical_list: D002130:Calcium Phosphates; D011091:Polyesters; C485817:beta-tricalcium phosphate; D014867:Water; C016240:polycaprolactone; D003094:Collagen