title: Involvement of superoxide dismutase in oxidative stress in the oriental fruit fly, Bactrocera dorsalis: molecular cloning and expression profiles.
abstract: BACKGROUND
  Bactrocera dorsalis, one of the most economically important fruit fly pests in East Asia, is well adapted to various environmental conditions. Pesticides, pathogens and other stresses can cause oxidative damage in most organisms. The superoxide dismutase (SOD) family contains some of the most important enzymes in the antioxidant protection system of the fruit fly and other organisms.
  
  
  RESULTS
  Four full-length cDNA sequences encoding one MnSOD (BdSOD2-1) and three Cu-ZnSODs (BdSOD1-1, BdSOD1-2 and BdSOD1-3) were cloned. The expression profiles of these four genes under different stresses showed them to be involved in response to detrimental conditions including heavy metals, pesticides, extreme temperatures and lipopolysaccharide (LPS) stresses. More specifically, the expression levels of these genes were found to be depressed in the presence of copper, zinc and manganese. The expression of all four SOD genes increased upon exposure to lead, cadmium, low temperature (0 °C) and LPS stresses. Only BdSOD1-3 transcription increased significantly at high temperature (40 °C) exposure. The expressions levels of BdSOD1-2 and BdSOD1-3 increased significantly in the presence of β-cypermethrin and malathion, but only the expression of BdSOD2-1 increased in the presence of avermectin treatment.
  
  
  CONCLUSIONS
  These different expression profiles suggest that the four BdSODs play different roles and respond to different oxidative stresses in B. dorsalis. Some BdSODs undergo specific reaction in the response to specific oxidative stresses.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, PR China.
authors.forename: Xue-Meng
authors.initials: XM
authors.lastname: Gao
authors.forename: Fu-Xian
authors.initials: FX
authors.lastname: Jia
authors.forename: Guang-Mao
authors.initials: GM
authors.lastname: Shen
authors.forename: Hua-Qin
authors.initials: HQ
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Dou
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010802:Phylogeny; D016415:Sequence Alignment; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D033621:Tephritidae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D000099156:Superoxide Dismutase 2
chemical_list: D019476:Insect Proteins; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2
keywords: Bactrocera dorsalis; RT-qPCR; cloning; oxidative stress; superoxide dismutase