title: Highly sensitive localized surface plasmon resonance immunosensor for label-free detection of HIV-1.
abstract: A highly sensitive label-free immunosensor for the detection of HIV-1 is newly developed based on localized surface plasmon resonance (LSPR) method. Uniform nanopattern of circular Au-dots (10-20nm) was fabricated on indium tin oxide (ITO) coated glass substrate by simple electrochemical deposition method. The surface of Au nanopattern was modified with HIV-1 neutralizing gp120 monoclonal antibody fragments. The modified substrate was employed to measure various concentrations of HIV-1 particles quantitatively based on the shift of longitudinal wavelength in the UV-Vis spectrum which results from the changes of local refractive index induced by specific antigen-antibody recognition events. The detection limit of the HIV-1 particles was estimated to be 200fg/mL, which is 10 fold higher than that of previously reported virus detection method based on LSPR. Since fabricated LSPR immunosensor has high sensitivity and selectivity, it is a promising approach for biological/medical sample analysis and various kinds of virus detection.
  
  
  BACKGROUND
  A localized surface plasmon resonance-based virus detection method is shown to have an order of magnitude improvement in detectable concentration of HIV-1 particles. Similar approaches may be used for screening other viral particles as well.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Mapo-Gu,, Seoul, 121-742, Republic of Korea; Research Institute for Basic Science, Sogang University, Mapo-Gu, Seoul, 121-742, Republic of Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung-Chan
authors.initials: BC
authors.lastname: Kim
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral / Q000235:genetics; D006046:Gold; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D053758:Nanoparticles / Q000648:ultrastructure; D055620:Optical Phenomena; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D014771:Virion / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D006046:Gold
keywords: AIDS; Biosensor; HIV-1 virus; Immunosensor; Localized surface plasmon resonance