title: HLA-B*5701 genetic screening among HIV-1 infected patients in Hong Kong: is this a practical approach in Han-Chinese?
abstract: Abacavir hypersensitivity is associated with the presence of human leukocyte antigen (HLA)-allele B*5701. However, the cost and workload of routine HLA-B*5701 pretreatment screening is relatively heavy. This study aimed to determine the prevalence of the HLA-B*5701 allele in the HIV-positive population under care in Hong Kong. Blood samples from 1264 HIV-1 infected patients in Hong Kong were collected between 2007 and 2011 for this study. HLA-B*5701 screening of the study group was determined by in-house polymerase chain reaction (PCR) followed by confirmation using the AlleleSEQR(®) HLA-B PCR/Sequencing Kit (Celera Corporation for Abbott, San Francisco, USA). HLA-B*5701 carriers were identified among 3% of Caucasians, 1% of non-Chinese Asians and 0.5% of Han-Chinese in Hong Kong. Our findings revealed that HLA-B*5701 pretreatment screening might not be necessary for the local Han-Chinese population due to its low prevalence.
authors.affiliation: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong.
authors.forename: S W C
authors.initials: SW
authors.lastname: To
authors.forename: J H K
authors.initials: JH
authors.lastname: Chen
authors.forename: K H
authors.initials: KH
authors.lastname: Wong
authors.forename: K C W
authors.initials: KC
authors.lastname: Chan
authors.forename: O T Y
authors.initials: OT
authors.lastname: Tsang
authors.forename: W C
authors.initials: WC
authors.lastname: Yam
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D015224:Dideoxynucleosides / Q000627:therapeutic use*; D004342:Drug Hypersensitivity / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D005820:Genetic Testing / Q000379:methods*; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology; D015497:HIV-1 / Q000235:genetics; D015235:HLA-B Antigens / Q000097:blood / Q000235:genetics*; D006723:Hong Kong; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018894:Reverse Transcriptase Inhibitors / Q000009:adverse effects* / Q000627:therapeutic use; D055815:Young Adult; D000099227:Abacavir
chemical_list: D015224:Dideoxynucleosides; D005819:Genetic Markers; D015235:HLA-B Antigens; D018894:Reverse Transcriptase Inhibitors; D000099227:Abacavir; C484830:HLA-B*57:01 antigen
keywords: HLA-B*5701; Han-Chinese; Hong Kong; abacavir hypersensitivity