title: Enhanced production of full-length immunoglobulin G via the signal recognition particle (SRP)-dependent pathway in Escherichia coli.
abstract: Because of the lack of post-translational glycosylation, Escherichia coli is not a preferable host for immunoglobulin G (IgG) production. However, recent successes in the developments of aglycosylated IgG variants that do not require glycosylation for effector functions have increased the likelihood of using E. coli for IgG production. Here, we have developed a new E. coli host-vector system for enhanced production of recombinant IgG using: (i) a combination of SRP/Sec-dependent pathways for the efficient secretion of heavy and light chains in the periplasm; (ii) co-expression of periplasmic foldase (DsbC) for efficient assembly of IgG in the periplasm; and (iii) co-expression of Ffh for enhancing the SRP machinery. Finally, with engineered host-vector system, fed-batch cultivations were conducted at four different conditions, and under an optimized condition, up to 62 mg/L of active full-length IgG was produced during a 28-h cultivation.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology-KAIST, Republic of Korea.
authors.forename: Yong Jae
authors.initials: YJ
authors.lastname: Lee
authors.forename: Hee Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Ae Jin
authors.initials: AJ
authors.lastname: Ryu
authors.forename: Ki Jun
authors.initials: KJ
authors.lastname: Jeong
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D018271:Signal Recognition Particle / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D007074:Immunoglobulin G; D018271:Signal Recognition Particle