title: Actinotalea ferrariae sp. nov., isolated from an iron mine, and emended description of the genus Actinotalea.
abstract: A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, designated strain CF5-4(T), was isolated from iron mining powder. 16S rRNA gene sequence analysis grouped strain CF5-4(T) in a single cluster with Actinotalea fermentans DSM 3133(T) (97.6% similarity). The major fatty acids (>5%) of strain CF5-4(T) were anteiso-C(15:0), anteiso-C(15:1) A, C(16:0), iso-C(16:0), iso-C(15:0) and anteiso-C(17:0). The predominant respiratory quinone was MK-10(H₄) and the genomic DNA G+C content was 74.7 mol%. The major polar lipids were diphosphatidylglycerol and one unidentified phosphoglycolipid. The peptidoglycan type of strain CF5-4(T) was A4β, containing L-Orn-D-Ser-D-Asp. The cell-wall sugars were rhamnose, fucose, mannose and galactose. The results of DNA-DNA hybridization in combination with the comparison of phenotypic and phylogenetic characteristics among strain CF5-4(T) and related micro-organisms revealed that the isolate represents a novel species of the genus Actinotalea, for which the name Actinotalea ferrariae sp. nov. is proposed. The type strain is CF5-4(T) ( =KCTC 29134(T) =CCTCC AB2012198(T)).
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Gejiao
authors.initials: G
authors.lastname: Wang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007501:Iron; D008906:Mining; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D007501:Iron