title: Expression of glutamine metabolism-related proteins according to molecular subtype of breast cancer.
abstract: The aim of this study was to investigate the expression of glutamine metabolism-related proteins to determine whether glutamine is metabolized differently according to breast cancer molecular subtype. We generated a tissue microarray of 702 breast cancer patients and performed immunohistochemical staining for glutamine metabolism-related proteins, including glutaminase 1 (GLS1 (GLS)), glutamate dehydrogenase (GDH (H6PD)), and amino acid transporter-2 (ASCT2 (SLC1A5)), which were separately evaluated in tumor and stroma compartments and then analyzed by breast cancer molecular subtypes. Breast cancers were classified as follows: 293 luminal A (41.7%), 166 luminal B (23.6%), 67 HER2 type (9.6%), and 176 TNBC (25.1%). HER2 type showed the highest stromal GLS1 (P=0.001), tumoral GDH (P=0.001), stromal GDH (P<0.001), and tumoral ASCT (P<0.001) expression. We identified differential expression of glutamine metabolism-related proteins according to molecular subtype of breast cancer. The highest glutamine metabolic activity was seen in HER2-type breast cancer.
authors.affiliation: Department of Pathology, Yonsei University Health System, Seoul, South Korea.
authors.forename: Sewha
authors.initials: S
authors.lastname: Kim
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Woo-Hee
authors.initials: WH
authors.lastname: Jung
authors.forename: Ja Seung
authors.initials: JS
authors.lastname: Koo
mesh_terms: D000328:Adult; D026962:Amino Acid Transport System ASC / Q000378:metabolism*; D001943:Breast Neoplasms / Q000145:classification / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000145:classification / Q000378:metabolism*; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D005972:Glutaminase / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens; D010641:Phenotype; D046888:Tissue Array Analysis
chemical_list: D026962:Amino Acid Transport System ASC; D015778:Minor Histocompatibility Antigens; C420506:SLC1A5 protein, human; D005973:Glutamine; D005969:Glutamate Dehydrogenase; C579480:GLS protein, human; D005972:Glutaminase
keywords: breast cancer; glutamine; metabolism