title: Accumulation of phenolic compounds in in vitro cultures and wild plants of Lavandula viridis L'Hér and their antioxidant and anti-cholinesterase potential.
abstract: In this study, we evaluated the phenolic profile, antioxidant and anti-cholinesterase potential of different extracts from wild plants and in vitro cultures of Lavandula viridis L'Hér. The HPLC-DAD analysis allowed the identification and quantification of 3-O-caffeoylquinic, 4-O-caffeoylquinic, 5-O-caffeoylquinic and rosmarinic acids, and luteolin and pinocembrin. Water/ethanol extract from in vitro cultures contained the highest amount of the identified phenolic compounds (51652.92 mg/kg). To investigate the antioxidant activity we used Trolox equivalent antioxidant capacity, oxygen radical absorbance capacity, Fe(2+) chelation activity and the inhibition of Fe(2+)-induced lipid peroxidation in mouse brain homogenates (in vitro). Overall, all the extracts from both wild plants and in vitro cultures exhibited ability to scavenge free radicals, to chelate Fe(2+) and to protect against lipid peroxidation. In addition, the extracts from L. viridis were active in inhibiting both acetylcholinesterase and butyrylcholinesterase (Ellman's method). Our findings suggest that L. viridis in vitro cultures represent a promising alternative for the production of active metabolites with antioxidant and anti-cholinesterase activity.
authors.affiliation: IBB-CGB, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139 Faro, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Costa
authors.forename: Sandra
authors.initials: S
authors.lastname: Gonçalves
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.forename: Anabela
authors.initials: A
authors.lastname: Romano
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002934:Cinnamates / Q000378:metabolism; D053630:Depsides / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology; D027523:Lavandula / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D046509:Tissue Culture Techniques; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D002934:Cinnamates; D053630:Depsides; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010636:Phenols; D010936:Plant Extracts; D000097123:Rosmarinic Acid