title: Spectroscopic characterization of i-motif forming c-myc derived sequences double-labeled with pyrene.
abstract: In current studies we use the oligonucleotides based on c-myc sequence: CCC CAC CCT CCC CAC CCT CCC C (cmyc22) and CCC CAC CCT CCC CAC CCT CCC CA (cmyc22A) functionalized by pyrene moieties at both termini. Results of the circular dichroism (CD), UV absorption melting experiments, and steady-state fluorescence measurements of pyrene-modified i-motifs as well as their unlabeled precursors are presented and discussed here. The pyrene labels have a remarkable influence on i-motif stability which was deduced from CD spectra and confirmed by UV melting experiments. Both probes emit fluorescence band of pyrene monomer with intensity decreasing upon pH lowering.
authors.affiliation: Laboratory of Bioanalytical Chemistry, Faculty of Chemistry, A. Mickiewicz University, Umultowska 89b, 61-614, Poznań, Poland. aniojka@amu.edu.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Dembska
authors.forename: Patrycja
authors.initials: P
authors.lastname: Rzepecka
authors.forename: Bernard
authors.initials: B
authors.lastname: Juskowiak
mesh_terms: D001483:Base Sequence; D059372:Nucleotide Motifs; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D011721:Pyrenes / Q000737:chemistry*; D013057:Spectrum Analysis; D044366:Transition Temperature
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D011721:Pyrenes; C030984:pyrene