title: New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris.
abstract: Biopharmaceuticals are an integral part of modern medicine and pharmacy. Both, the development and the biotechnological production of biopharmaceuticals are highly cost-intensive and require suitable expression systems. In this review we discuss established and emerging tools for reengineering the methylotrophic yeast Pichia pastoris for biopharmaceutical production. Recent advancements of this industrial expression system through synthetic biology include synthetic promoters to avoid methanol induction and to fine-tune protein production. New platform strains and molecular cloning tools as well as in vivo glycoengineering to produce humanized glycoforms have made P. pastoris an important host for biopharmaceutical production.
authors.affiliation: Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz A-8010, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Vogl
authors.forename: Franz S
authors.initials: FS
authors.lastname: Hartner
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
mesh_terms: D000818:Animals; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D001709:Biotechnology / Q000379:methods*; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D006031:Glycosylation; D006801:Humans; D000432:Methanol / Q000378:metabolism / Q000494:pharmacology; D010843:Pichia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D001688:Biological Products; D006023:Glycoproteins; D000432:Methanol