title: Glucosinolate biosynthesis in hairy root cultures of broccoli (Brassica oleracea var. italica).
abstract: Here we present previously unreported glucosinolate production by hairy root cultures of broccoli (B. oleracea var. italica). Growth media greatly influenced the growth and glucosinolate content of hairy root cultures of broccoli. Seven glucosinolates, glucoraphanin, gluconapin, glucoerucin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin, were identified by analysis of the broccoli hairy root cultures. Both half and full strength B5 and SH media enabled the highest accumulation of glucosinolates. In most cases, the levels of glucosinolates were higher in SH and BS media. Among the 7 glucosinolates, the accumulation of neoglucobrassicin was very high, irrespective of growth medium. The neoglucobrassicin content was 7.4-fold higher in SH medium than 1/2 MS, in which its level was the lowest. The 1/2 B5 medium supported the production of the highest amounts of glucobrassicin and 4-methoxyglucobrassicin, the levels for which were 36.2- and 7.9- fold higher, respectively, than their lowest content in 1/2 MS medium. The 1/2 SH medium enabled the highest accumulation of glucoraphanin and gluconapin in the broccoli hairy root cultures, whose levels were 1.8- and 4.6-fold higher, respectively, than their lowest content in 1/2 MS medium. Our results suggest that hairy root cultures of broccoli could be a valuable alternative approach for the production of glucosinolate compounds.
authors.affiliation: Department of Bio-Environmental Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 305-764, Republic of Korea.
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Woo Tae
authors.initials: WT
authors.lastname: Park
authors.forename: Md Romij
authors.initials: MR
authors.lastname: Uddin
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Nam
authors.forename: Kwang Hyun
authors.initials: KH
authors.lastname: Jho
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D001937:Brassica / Q000254:growth & development / Q000378:metabolism*; D005961:Glucosinolates / Q000096:biosynthesis*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism
chemical_list: D005961:Glucosinolates