title: Size-dependent photodynamic activity of gold nanoparticles conjugate of water soluble purpurin-18-N-methyl-d-glucamine.
abstract: Gold nanoparticles (GNPs) conjugates of water soluble ionic photosensitizer (PS), purpurin-18-N-methyl-D-glucamine (Pu-18-NMGA), were synthesized using various molar ratios between HAuCl4 and Pu-18-NMGA without adding any particular reducing agents and surfactants. The PS-GNPs conjugates showed long wavelength absorption of range 702-762 nm, and their different shapes and diameters depend on the molar ratios used in the synthesis. In vitro anticancer efficacy of the PS-GNPs conjugates was investigated by MTT assay against A549 cells, resulting in higher photodynamic activity than that of the free Pu-18-NMGA. Among the PS-GNPs conjugates, the GNPs conjugate from the molar ratio of 1 : 2 (Au(III): Pu-18-NMGA) exhibits the highest photodynamic activity corresponding to bigger size (~60 nm) of the GNPs conjugate which could efficiently transport the PS into the cells than that of smaller size of the GNPs conjugate.
authors.affiliation: PDT Research Institute, School of Nano System Engineering, Inje University, Gimhae 621-749, Republic of Korea.
authors.forename: Byambajav
authors.initials: B
authors.lastname: Lkhagvadulam
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Il
authors.initials: I
authors.lastname: Yoon
authors.forename: Young Key
authors.initials: YK
authors.lastname: Shim
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D045744:Cell Line, Tumor; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D011166:Porphyrins / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D011166:Porphyrins; D014867:Water; C059051:purpurin 18; D006046:Gold