title: Quantitative assessment of tumor blood flow changes in a murine breast cancer model after adriamycin chemotherapy using contrast-enhanced destruction-replenishment sonography.
abstract: OBJECTIVE
  The purpose of the study was to detect tumor blood flow changes after chemotherapy with contrast-enhanced destruction-replenishment sonography.
  
  
  METHODS
  Twenty-four MCF-7 breast cancer-bearing nude mice were included in this study. Animals received either adriamycin or sterile saline and underwent contrast-enhanced sonography before and after treatment using a destruction-replenishment technique. A monoexponential function, y = A(1 - e(-βt)), was used to fit the replenishment kinetics, where the plateau signal intensity A reflects the percent blood volume; the time constant β reflects the average speed of blood; and their product A*β reflects the nutrient blood flow. Tumor blood perfusion was compared to measurements of cell density and microvascular density.
  
  
  RESULTS
  Volumes of the treated tumors were significantly reduced after 7 days of adriamycin treatment compared with the control tumors (P < .001). Before adriamycin administration, there was no significant difference in blood perfusion between the treated and control groups (P > .05). Treatment with adriamycin resulted in a significant decrease in A, β, and A*β (P <.001) compared with the control tumors. The tumor cell density and microvascular density estimated by pathologic slices were significantly lower in the treated tumors than in the control tumors (P <.001).
  
  
  CONCLUSIONS
  Quantification of tumor blood flow using contrast-enhanced destruction-replenishment sonography shows the potential to evaluate tumor responses to chemotherapy.
authors.affiliation: Department of Ultrasound, State Key Laboratory of Oncology in Southern China, Sun Yat-Sen University Cancer Center, Guangzhou, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.forename: Long-Hui
authors.initials: LH
authors.lastname: Cao
authors.forename: Rong-Zhen
authors.initials: RZ
authors.lastname: Luo
authors.forename: An-Hua
authors.initials: AH
authors.lastname: Li
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging* / Q000188:drug therapy; D002452:Cell Count; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000627:therapeutic use; D005260:Female; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008819:Mice, Nude; D012039:Regional Blood Flow; D014463:Ultrasonography / Q000379:methods
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin