title: Nanodiamond based sponges with entrapped enzyme: a novel electrochemical probe for hydrogen peroxide.
abstract: A "modular approach" has been demonstrated for the preparation of nanodiamond (ND) based sponges (NS) with entrapped enzyme (horseradish peroxidase, HRP) (ND-NS(HRP)) and utilization as an electrochemical probe for detection of hydrogen peroxide (H₂O₂). ND-NS comprises ND, porous poly(aniline)-poly(2-acrylamido 2-methyl propane sulfonic acid) (PANI-PAMPSA) network and entrapped HRP. Field emission scanning electron microscope image of ND-NS(HRP) reveals sponge like suprastructure comprising interconnected nanospheres with numerous openings/pinholes/cavities. The entrapped HRP in ND-NS exhibits effective direct electron transfer with an electron transfer rate constant of 1.85 s(-1). ND-NS(HRP) exhibited excellent bioelectrocatalytic reduction of hydrogen peroxide (H₂O₂) with a wide linear concentration range (1-45 mM), quick response (5s), high sensitivity (129.6 μAM(-1)) and low detection limit 59 μM (S/N=3).
authors.affiliation: Department of Chemistry Education, Kyungpook National University, Republic of Korea.
authors.forename: Anantha Iyengar
authors.initials: AI
authors.lastname: Gopalan
authors.forename: Shanmugasundaram
authors.initials: S
authors.lastname: Komathi
authors.forename: Gopalan
authors.initials: G
authors.lastname: Sai Anand
authors.forename: Kwang-Pill
authors.initials: KP
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D058612:Nanodiamonds / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D004800:Enzymes, Immobilized; D058612:Nanodiamonds; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase