title: Effect of interfacial serum proteins on melanoma cell adhesion to biodegradable poly(l-lactic acid) microspheres coated with hydroxyapatite.
abstract: We have measured the interaction forces between a murine melanoma cell and a poly(l-lactic acid) (PLLA) microsphere coated with/without hydroxyapatite (HAp) nanoparticles (i.e., an HAp/PLLA or a bare PLLA microsphere) in a serum-free culture medium, using atomic force microscopy (AFM) with colloid probe technique, in order to investigate how the HAp-nanoparticle coating as well as interfacial serum proteins influence the cell-microsphere adhesion. The cell adhesion force of the HAp/PLLA microspheres was 1.4-fold stronger than that of the bare PLLA microspheres. When the microspheres were pretreated with a culture medium supplemented with 10% fetal bovine serum, the cell adhesion force of the HAp/PLLA microspheres was increased by a factor of 2.1; in contrast, no change was observed in the cell adhesion force of the bare PLLA microspheres before/after the pretreatment. Indeed, the cell adhesion force of the HAp/PLLA was 2.8-fold larger than that of the bare PLLA after the pretreatment. Additionally, we have investigated the effect of interfacial serum proteins on the zeta potentials of these microspheres. On the basis of the obtained results, possible mechanism of cell adhesion to the HAp/PLLA and bare PLLA microspheres in the presence/absence of the interfacial serum proteins is discussed.
authors.affiliation: Department of Chemical Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. shinto@cheme.kyoto-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shinto
authors.forename: Takuya
authors.initials: T
authors.lastname: Hirata
authors.forename: Tomonori
authors.initials: T
authors.lastname: Fukasawa
authors.forename: Syuji
authors.initials: S
authors.lastname: Fujii
authors.forename: Hayata
authors.initials: H
authors.lastname: Maeda
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Furuzono
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D003102:Colloids; D017886:Durapatite / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008546:Melanoma, Experimental; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D001798:Blood Proteins; D003102:Colloids; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite