title: Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury.
abstract: BACKGROUND
  Reestablishment of functional networks after traumatic brain injury (TBI) has been proffered as one of the goals of neural stem cell (NSC) transplantation therapeutics. Gap junctions provide essential means for direct cellular communication by transferring small molecules and ions, which may provide insights into the interplay between grafted NSCs and host cells.
  
  
  METHODS
  Thirty-six adult male Wister rats were used in this study. The controlled cortical impact (CCI) model of brain injury has been performed. Seventy-two hours after CCI injury, animals were randomly assigned to two groups: PBS- and NSC- transplanted group. NSCs-transplanted group received delivery of the NSCs suspension to the cortex below the injury cavity in the ipsilateral hemisphere. At 1, 2, and 4 weeks post-transplantation, we investigated the expression patterns of gap junction-associated connexin 43 (Cx43) in the transplant site and the border of CCI by immunohistochemistry, Western blot and RT-PCR.
  
  
  RESULTS
  Our findings showed that Cx43 staining was significantly greater in the transplant site and the border of CCI in the NSCs-transplanted rats compared to the control rats at different time points (p < 0.01 at 1 week, p < 0.05 at 2 and 4 weeks). Significantly higher gene and protein expression of Cx43 was found in NSCs-transplanted rats compared to the control rats in the period of 4 weeks post-transplantation (p < 0.01), and remained at a higher level until 2 weeks with or without NSC transplantation.
  
  
  CONCLUSIONS
  It is proposed that gap junction-associated Cx43 might participate in NSCs' beneficial effects via gap-junctional coupling by which grafted NSCs integrate into host neural tissue following transplantation after TBI.
authors.affiliation: Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Haiying
authors.initials: H
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Kong
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Shi
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Liu
keywords: connexin43; neural stem cell; transplantation; traumatic brain injury