title: Crlz-1 is prominently expressed in spermatogonia and Sertoli cells during early testis development and in spermatids during late spermatogenesis.
abstract: The expression of the Crlz-1 gene in mouse testis, where it was found to be expressed most highly among the tested mouse organs, was analyzed spatiotemporally by employing RT-PCR and in situ hybridization techniques with the aid of immunohistochemistry and/or immunofluorescence methods. In 1-week-old neonatal testis, Crlz-1 was strongly expressed in the spermatogonia and Sertoli cells in its seminiferous cord. In 2- to 3-week-old prepubertal testis, where Sertoli cells cease to proliferate, Crlz-1 expression dropped and remained weakly at the rim layer of seminiferous cords and/or tubules, where spermatogonia are present. In the adult testis at 12 weeks after birth, Crlz-1 was expressed mainly in the spermatids near the lumen of seminiferous tubules. In a further in situ hybridization of Crlz-1 in the 12-week-old adult testis with hematoxylin nuclear counterstaining, Crlz-1 was mainly expressed at step 16 of spermatids between stages VII and VIII of seminiferous tubules as well as in their residual bodies at stage IX of seminiferous tubules.
authors.affiliation: Department of Genetic Engineering, College of Life Sciences, Kyung Hee University, Republic of Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lim
authors.forename: Seong-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Han-Woong
authors.initials: HW
authors.lastname: Yoo
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Cho
authors.forename: Youngsook
authors.initials: Y
authors.lastname: Son
authors.forename: Chang-Joong
authors.initials: CJ
authors.lastname: Kang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D050657:Core Binding Factor alpha Subunits / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D012671:Seminiferous Tubules / Q000254:growth & development / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D013087:Spermatids / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D050657:Core Binding Factor alpha Subunits; C435748:Crlz1 protein, mouse; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin
keywords: Crlz-1; Sertoli cell; seminiferous tubule; spermatid; spermatogonia