title: Nanopatterned polymer brushes as switchable bioactive interfaces.
abstract: We report convenient methods for synthesis of nanopatterned, thermally responsive brushes of poly(N-isopropyl acrylamide) over large areas (e.g., 1 cm(2)) to form model, dynamic, biofunctional surfaces. The new nanopatterned brush structure can be used to control (i) the rate of both nonspecific and biospecific adsorption processes at the polymer-graft-free regions of the substrate, and (ii) the rate of cell detachment. These capabilities have potential implications in a number of areas of biotechnology including biosensing, separations and cell culture.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Phanindhar
authors.initials: P
authors.lastname: Shivapooja
authors.forename: Leah M
authors.initials: LM
authors.lastname: Johnson
authors.forename: Getachew
authors.initials: G
authors.lastname: Tizazu
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Leggett
authors.forename: Gabriel P
authors.initials: GP
authors.lastname: López
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000378:metabolism; D000180:Acrylic Resins; D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D002448:Cell Adhesion; D006863:Hydrogen-Ion Concentration; D008027:Light; D051379:Mice; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D012710:Serum Albumin, Bovine