title: The biophysical property of A549 cells transferred by VEGF-D.
abstract: Vascular endothelial growth factor-D (VEGF-D) together with VEGF-C is considered to be associated with lymphangiogenesis and angiogenesis and involve in tumorization. This study aims to investigate the influence of exogenous VEGF-D gene on the biophysical property of cell surface of lung adenocarcinoma cell line. A panel of lung adenocarcinoma cell lines were examined the expression of VEGF-D and VEGF-C by real-time PCR. The VEGF-D recombinant plasmid containing enhanced green fluorescence protein (EGFP) was constructed and transfected to the cell line with no expression of VEGF-D and confirmed by real-time PCR and Western blot analysis. Topographic images of cells were obtained by using atomic force microscope (AFM) in contact mode. Unlike VEGF-C, VEGF-D was found to have a very low expression or undetectable expression in lung adenocarcinoma cell lines. The VEGF-D recombinant plasmid had been constructed successfully and was transferred into the human lung adenocarcinoma cell line A549 cells which had no endogenous expression of VEGF-D, and exogenous VEGF-D could be detected in mRNA and protein expression levels in the gene modified cells, while the VEGF-C gene expression had no change after VEGF-D transfection. After transfection, the irregular microspikes or nano clusters could observe on the surface of A549 cells, and VEGF-D transfected A549 cells became more rigid. The exogenous VEGF-D gene might cause the remarkable biophysical architectural changes in the A549 cells, which might as a novel biomarker for evaluation of its biological function.
authors.affiliation: Guangdong Lung Cancer Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, PR China.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wu
authors.affiliation: Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Guangdong Lung Cancer Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, PR China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Tian
authors.affiliation: Guangdong Lung Cancer Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, PR China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, PR China.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, PR China.
authors.forename: Yang-Qiu
authors.initials: YQ
authors.lastname: Li
mesh_terms: D055592:Biophysical Phenomena; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000502:physiology* / Q000648:ultrastructure; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D018625:Microscopy, Atomic Force; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics / Q000378:metabolism; D042643:Vascular Endothelial Growth Factor D / Q000235:genetics / Q000378:metabolism*
chemical_list: D042582:Vascular Endothelial Growth Factor C; D042643:Vascular Endothelial Growth Factor D
keywords: VEGF‐D; atomic force microscope; gene transfer; lung cancer