title: Evaluation of the effects of homologous platelet gel on healing lower extremity wounds in patients with diabetes.
abstract: The treatment of chronic diabetic wounds remains complicated, despite new insight into the cellular and molecular basis of wound healing and cutaneous regeneration. A growing body of clinical trials has shown that platelet release has a notable effectiveness on refractory ulcer healing. However, patients with chronic diabetic ulcers usually have poor general health, and the large-volume blood absence required to produce autologous platelet-rich plasma often causes adverse effects. To overcome the limitation, the homologous platelet gel (PG) from healthy donor was used for the treatment of chronic diabetic lower extremity wound in the study. We show here that homologous derived platelets significantly enhanced EVC304 cell and HaCaT cell proliferation and homologous PG was capable of prompting cell migration. Twenty-one patients with refractory diabetic lower extremity ulcers, who had no response to conventional treatments, were treated in this study. Our data indicated that homologous PG was effective for the enhancement and acceleration of diabetic lower extremity wounds healing. We propose that homologous PG appeared to enhance vascularization and epithelialization, which might induce a quicker healing process and and encourage controlled studies in future.
authors.affiliation: Southern Medical University, Guangzhou, China.
authors.forename: Gui-Qiu
authors.initials: GQ
authors.lastname: Shan
authors.forename: Ya-Ni
authors.initials: YN
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Da-Ming
authors.initials: DM
authors.lastname: Zuo
authors.forename: Jin-Lang
authors.initials: JL
authors.lastname: Qiu
authors.forename: Biao
authors.initials: B
authors.lastname: Cheng
authors.forename: Zheng-Liang
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002460:Cell Line; D017719:Diabetic Foot / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D042783:Endothelial Cells / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D005782:Gels; D006801:Humans; D015603:Keratinocytes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D053657:Platelet-Rich Plasma; D012189:Retrospective Studies; D016896:Treatment Outcome; D018608:Ultrasonography, Doppler; D014945:Wound Healing
chemical_list: D005782:Gels