title: Crystallization and preliminary X-ray diffraction analysis of three recombinant mutants of Vaccinia virus uracil DNA glycosylase.
abstract: Amino-acid residues located at a highly flexible area in the uracil DNA glycosylase of Vaccinia virus were mutated. In the crystal structure of wild-type D4 these residues lie at the dimer interface. Specifically, three mutants were generated: (i) residue Arg167 was replaced with an alanine (R167AD4), (ii) residues Glu171, Ser172 and Pro173 were substituted with three glycine residues (3GD4) and (iii) residues Glu171 and Ser172 were deleted (Δ171-172D4). Mutant proteins were expressed, purified and crystallized in order to investigate the effects of these mutations on the structure of the protein.
authors.affiliation: Science and Technology Honors Program, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Darika
authors.initials: D
authors.lastname: Sartmatova
authors.forename: Taishayla
authors.initials: T
authors.lastname: Nash
authors.forename: Norbert
authors.initials: N
authors.lastname: Schormann
authors.forename: Manunya
authors.initials: M
authors.lastname: Nuth
authors.forename: Robert
authors.initials: R
authors.lastname: Ricciardi
authors.forename: Surajit
authors.initials: S
authors.lastname: Banerjee
authors.forename: Debasish
authors.initials: D
authors.lastname: Chattopadhyay
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D051981:Uracil-DNA Glycosidase / Q000737:chemistry* / Q000235:genetics; D014616:Vaccinia virus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D000596:Amino Acids; D011994:Recombinant Proteins; D014764:Viral Proteins; D051981:Uracil-DNA Glycosidase
keywords: Vaccinia virus; uracil DNA glycosylase