title: Phytochemical, antioxidant and protective effect of Rhus tripartitum root bark extract against ethanol-induced ulcer in rats.
abstract: Rhus tripartitum (sumac) is an Anacardiaceae tree with a wide phytotherapeutic application including the use of its roots in the management of gastric ulcer. In the present study the Rhus tripartitum root barks extract (RTE) was phytochemical studied, in vitro tested for their potential antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay and in vivo evaluated for its ability to prevent ethanol-induced gastric ulcer in rats. The RTE was rich in phenolics, flavonoids, tannins and polysaccharide contents and exhibited a low but not weak in vitro antioxidant activity when compared with (+)-catechin. Pre-treatment with RTE at oral doses 50, 200 and 400 mg/kg body weight was found to provide a dose-dependent protection against ethanol-induced ulcer by averting the deep ulcer lesions of the gastric epithelium, by reducing gastric juice and acid output, by enhancing gastric mucus production by preserving normal antioxidant enzymes activities, and inhibiting the lipid peroxidation. The antiulcerogenic activity of RTE might be due to a possible synergistic antioxidant and antisecretory effects.
authors.affiliation: Research unit of Macromolecular Biochemistry and Genetic, Faculty of Sciences of Gafsa, Gafsa, Tunisia. alimihichem@yahoo.fr
authors.forename: Hichem
authors.initials: H
authors.lastname: Alimi
authors.forename: Sakhria
authors.initials: S
authors.lastname: Mbarki
authors.forename: Zeineb B
authors.initials: ZB
authors.lastname: Barka
authors.forename: Anwer
authors.initials: A
authors.lastname: Feriani
authors.forename: Zouhour
authors.initials: Z
authors.lastname: Bouoni
authors.forename: Najla
authors.initials: N
authors.lastname: Hfaeidh
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.forename: Olfa
authors.initials: O
authors.lastname: Tebourbi
authors.forename: Khémais B
authors.initials: KB
authors.lastname: Rhouma
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000009:adverse effects; D005419:Flavonoids / Q000737:chemistry; D008297:Male; D019800:Phenol / Q000737:chemistry; D008517:Phytotherapy / Q000379:methods; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D029045:Rhus / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D013634:Tannins / Q000737:chemistry; D013997:Time Factors; D014456:Ulcer / Q000188:drug therapy*
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D011134:Polysaccharides; D013634:Tannins; D019800:Phenol; D000431:Ethanol