title: In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
abstract: Expressed prostatic secretions (EPS) are proximal fluids of the prostate that are increasingly being utilized as a clinical source for diagnostic and prognostic assays for prostate cancer (PCa). These fluids contain an abundant amount of microvesicles reflecting the secretory function of the prostate gland, and their protein composition remains poorly defined in relation to PCa. Using expressed prostatic secretions in urine (EPS-urine), exosome preparations were characterized by a shotgun proteomics procedure. In pooled EPS-urine exosome samples, ~900 proteins were detected. Many of these have not been previously observed in the soluble proteome of EPS generated by our labs or other related exosome proteomes. We performed systematic comparisons of our data against previously published, prostate-related proteomes, and global annotation analyses to highlight functional processes within the proteome of EPS-urine derived exosomes. The acquired proteomic data have been deposited to the Tranche repository and will lay the foundation for more extensive investigations of PCa derived exosomes in the context of biomarker discovery and cancer biology.
authors.affiliation: Ontario Cancer Institute, University Health Network, Toronto, Canada.
authors.forename: Simona
authors.initials: S
authors.lastname: Principe
authors.affiliation: Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA.
authors.forename: E Ellen
authors.initials: EE
authors.lastname: Jones
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto, Canada.
authors.forename: Yunee
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto, Canada.
authors.forename: Ankit
authors.initials: A
authors.lastname: Sinha
authors.affiliation: Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA.|The Leroy T. Canoles Jr., Cancer Research Center, Eastern Virginia Medical School Norfolk, VA, USA.
authors.forename: Julius O
authors.initials: JO
authors.lastname: Nyalwidhe
authors.affiliation: Ontario Cancer Institute, University Health Network, Toronto, Canada.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Brooks
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto, Canada.|Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA.
authors.forename: O John
authors.initials: OJ
authors.lastname: Semmes
authors.affiliation: Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA.|The Leroy T. Canoles Jr., Cancer Research Center, Eastern Virginia Medical School Norfolk, VA, USA.
authors.forename: Dean A
authors.initials: DA
authors.lastname: Troyer
authors.affiliation: The Leroy T. Canoles Jr., Cancer Research Center, Eastern Virginia Medical School Norfolk, VA, USA.
authors.forename: Raymond S
authors.initials: RS
authors.lastname: Lance
authors.affiliation: Ontario Cancer Institute, University Health Network, Toronto, Canada.|Department of Medical Biophysics, University of Toronto, Toronto, Canada.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kislinger
authors.affiliation: Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Drake
mesh_terms: D016022:Case-Control Studies; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000652:urine; D011507:Proteinuria / Q000652:urine; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D020543:Proteome