title: Immunomodulating activities of cultivated maitake medicinal mushroom Grifola frondosa (Dicks.: Fr.) S.F. Gray (higher Basidiomycetes) on peripheral blood mononuclear cells.
abstract: Grifola frondosa is a culinary-medicinal mushroom that contains several physiologically active compounds, of which polysaccharides, specifically β-glucans, are known to possess immunomodulating properties. Its extracts are studied for application as adjuncts for chemotherapy, and experiments in animal models support the use of this mushroom for cancer treatment. The effect of extracts obtained from mushrooms cultivated on different substrates and their capacity of inducing the secretion of cytokines from human peripheral blood mononuclear cells were studied. The activity of extracts at concentrations 12.5, 100, and 200 μg/mL on induction of TNF-α, IFN-γ, and IL-12 was screened. Two extracts from substrates fortified with olive oil press cakes showed appreciable activity and induced the secretion of TNF-α, IL-12, and INF-γ. The extracts differed from the others in the amount of sugar, protein, and β-glucans, which can explain their higher activity. Present results show that different substrates and different source materials can reasonably modify the bioactivity of cultivated G. frondosa.
authors.affiliation: Department of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana.
authors.forename: Mirjan
authors.initials: M
authors.lastname: Svagelj
authors.forename: Marin
authors.initials: M
authors.lastname: Berovic
authors.forename: Andrej
authors.initials: A
authors.lastname: Gregori
authors.forename: Branka
authors.initials: B
authors.lastname: Wraber
authors.forename: Sasa
authors.initials: S
authors.lastname: Simcic
authors.forename: Bojana
authors.initials: B
authors.lastname: Boh
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D019649:Food Industry; D048690:Fruiting Bodies, Fungal / Q000254:growth & development; D005786:Gene Expression Regulation; D048691:Grifola / Q000737:chemistry*; D006801:Humans; D007220:Industrial Waste; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils; D014866:Waste Products
chemical_list: D016207:Cytokines; D007220:Industrial Waste; D000069463:Olive Oil; D010938:Plant Oils; D014866:Waste Products