title: Simultaneous determination of danshensu, salvianolic acid B, and paeonol in ShuangDan oral liquid by HPLC.
abstract: An HPLC-photodiode array detector (PDAD) method was developed for simultaneous determination of danshensu, salvianolic acid B, and paeonol in a traditional Chinese medicinal preparation ShuangDan oral liquid, which is widely used to treat coronary heart disease in China. The samples were separated on a SinoChrom ODS-BP C18 column with the column temperature maintained at 30 degrees C. The mobile phase was composed of methanol and 2% (v/v) glacial acetic acid at a flow rate of 1.0 mL/min. The separation was finished within 30 min using linear gradient elution. All calibration curves showed good linearity (r > 0.9997) within the test ranges. The method was simple, reliable, accurate, and specific. The intraday and interday precisions (RSD) were less than 0.85%. The recoveries were between 96.97 and 102.14%. The method was successfully applied to the determination of the three components in three commercial samples of ShuangDan oral liquid. The results indicated that the developed HPLC-PDAD assay could be readily utilized as a comprehensive quality control method for ShuangDan oral liquid.
authors.affiliation: The Fourth Military Medical University, School of Pharmacy, Institute of Materia Medica, 710032 Xi'an, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Siwang
authors.initials: S
authors.lastname: Wang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xie
authors.forename: Bangle
authors.initials: B
authors.lastname: Zhang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D000098:Acetophenones / Q000032:analysis*; D001572:Benzofurans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007773:Lactates / Q000032:analysis*; D053630:Depsides
chemical_list: D000098:Acetophenones; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D007773:Lactates; C521472:Shuangdan; C013638:paeonol; C035055:3,4-dihydroxyphenyllactic acid; C076944:salvianolic acid B; D053630:Depsides