title: Inhibitory effect of atorvastatin on AGE-induced HCAEC apoptosis by upregulating HSF-1 protein.
abstract: This study evaluates effect and mechanism of atorvastatin on human coronary artery endothelial cells (HCAEC) apoptosis. Results have shown that HCAEC apoptosis had increased with increasing concentration of AGEs. Atorvastatin may decrease HCAEC apoptosis, but the effect can be attenuated by PI3K inhibitor. Secretion of PI3K and P-Akt in HCAEC increased with increasing concentration of AGEs. Secretion of PI3K in HCAEC may be decreased by atorvastatin. The effect may be attenuated by PI3K inhibitor. HSF-1, HSP-70 mRNA expression level decreased with increasing concentration of AGEs. Atorvastatin may attenuate AGEs-induced HSF-1, HSP-70 mRNA expression in HCAEC, but the effect can be attenuated by PI3K inhibitor. It can be concluded that AGE can dose-dependently promote HCAEC apoptosis by the PI3K/AKT pathway. Atorvastatin may attenuate the effect possibly by upregulating HSF-1.
authors.affiliation: Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China. yingcnli@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jiming
authors.initials: J
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Yan
authors.initials: Y
authors.lastname: Lai
authors.forename: Yian
authors.initials: Y
authors.lastname: Yao
authors.forename: Yibo
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Pang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Xuebo
authors.initials: X
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000069059:Atorvastatin; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D000076249:Heat Shock Transcription Factors; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011758:Pyrroles / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D004268:DNA-Binding Proteins; D017127:Glycation End Products, Advanced; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011758:Pyrroles; D014157:Transcription Factors; D000069059:Atorvastatin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt