title: Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: effective photoinduced in vitro killing of osteosarcoma.
abstract: Mesenchymal stem cells (MSC) have the unique ability to home and engraft in tumor stroma. These features render them potentially a very useful tool as targeted delivery vehicles which can deliver therapeutic drugs to the tumor stroma. In the present study, we investigate whether fluorescent core-shell PMMA nanoparticles (FNPs) post-loaded with a photosensitizer, namely meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS) and uploaded by MSC could trigger osteosarcoma (OS) cell death in vitro upon specific photoactivation. In co-culture studies we demonstrate using laser confocal microscopy and time lapse imaging, that only after laser irradiation MSC loaded with photosensitizer-coated fluorescent NPs (TPPS@FNPs) undergo cell death and release reactive oxygen species (ROS) which are sufficient to trigger cell death of all OS cells in the culture. These results encourage further studies aimed at proving the efficacy of this novel tri-component system for PDT applications.
authors.affiliation: Osteoarticolar Regeneration Laboratory, Rizzoli Orthopaedic Institute IOR, Via di Barbiano 1/10, 40136, Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Duchi
authors.forename: G
authors.initials: G
authors.lastname: Sotgiu
authors.forename: E
authors.initials: E
authors.lastname: Lucarelli
authors.forename: M
authors.initials: M
authors.lastname: Ballestri
authors.forename: B
authors.initials: B
authors.lastname: Dozza
authors.forename: S
authors.initials: S
authors.lastname: Santi
authors.forename: A
authors.initials: A
authors.lastname: Guerrini
authors.forename: P
authors.initials: P
authors.lastname: Dambruoso
authors.forename: S
authors.initials: S
authors.lastname: Giannini
authors.forename: D
authors.initials: D
authors.lastname: Donati
authors.forename: C
authors.initials: C
authors.lastname: Ferroni
authors.forename: G
authors.initials: G
authors.lastname: Varchi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D006801:Humans; D059630:Mesenchymal Stem Cells; D053758:Nanoparticles / Q000008:administration & dosage; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011166:Porphyrins / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; D017382:Reactive Oxygen Species; C016136:tetraphenylporphine sulfonate