title: Molecularly imprinted polymer-based catalytic micromotors for selective protein transport.
abstract: We demonstrate an attractive nanomachine "capture and transport" target isolation strategy based on molecularly imprinted polymers (MIPs). MIP-based catalytic microtubular engines are prepared by electropolymerization of the outer polymeric layer in the presence of the target analyte (template). Tailor-made selective artificial recognition sites are thus introduced into the tubular microtransporters through complementary nanocavities in the outer polymeric layer. The new microtransporter concept is illustrated using bilayer poly(3,4-ethylenedioxythiophene) (PEDOT)/Pt-Ni microengines and fluorescein isothiocyanate (FITC)-labeled avidin (Av-FITC) as the template. The avidin-imprinted polymeric layer selectively concentrates the fluorescent-tagged protein target onto the moving microengine without the need for additional external functionalization, allowing "on-the-fly" extraction and isolation of Av-FITC from raw serum and saliva samples along with real-time visualization of the protein loading and transport. The new micromachine-MIP-based target isolation strategy can be extended to the capture and transport of other important target molecules, leading toward diverse biomedical and environmental applications.
authors.affiliation: Department of Nanoengineering, University of California, San Diego, La Jolla, California 92093, USA.
authors.forename: Jahir
authors.initials: J
authors.lastname: Orozco
authors.forename: Allan
authors.initials: A
authors.lastname: Cortés
authors.forename: Guanzhi
authors.initials: G
authors.lastname: Cheng
authors.forename: Sirilak
authors.initials: S
authors.lastname: Sattayasamitsathit
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Xiaomiao
authors.initials: X
authors.lastname: Feng
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Shen
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
mesh_terms: D001360:Avidin / Q000737:chemistry; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D002384:Catalysis; D006801:Humans; D017879:Isothiocyanates / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D054802:Molecular Imprinting; D009532:Nickel / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D017879:Isothiocyanates; D008051:Lipid Bilayers; D011108:Polymers; D011506:Proteins; C121383:poly(3,4-ethylene dioxythiophene); D001360:Avidin; C037152:isothiocyanic acid; D010984:Platinum; D009532:Nickel