title: Determination of enterotoxigenic and methicillin resistant Staphylococcus aureus in ice cream.
abstract: The aim of this study was to determine the prevalence of enterotoxigenic and methicillin-resistant Staphylococcus aureus in ice creams. After culture-based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32 vanilla (12.5%), and 1 of 12 chocolate (8.3%) ice creams. S. aureus was identified as 38 isolates in 23 ice cream samples by culture-based techniques, but only 35 isolates were confirmed by PCR as S. aureus. To determine the enterotoxigenic properties of PCR-confirmed S. aureus isolates, a toxin detection kit was used (SET RPLA®). Of the 12 enterotoxigenic S. aureus isolates, 9 SEB (75%), 1 SED (8.3%), 1 SEB+SED (8.3%), and 1 SEA+SEB+SED (8.3%) expressing isolates were found. The presence of enterotoxin genes (sea, seb, sed) was identified in 13 (37.1%) out of 35 isolates by the mPCR technique. In the ice cream isolates, the sea, seb, and sed genes were detected: 1 sea (7.6%), 9 seb (69.2%), 1 sed (7.6%), 1 seb+sed (7.6%), and 1 sea+seb+sed (7.6%), respectively. The sec gene was not detected in any of these isolates. One of the 35 (2.8%) S. aureus strain was mecA positive.
authors.affiliation: Dept. of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ondokuz Mayıs Univ., Kurupelit/Samsun, Turkey. aligucuk77@hotmail.com
authors.forename: Ali
authors.initials: A
authors.lastname: Gücükoğlu
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Çadirci
authors.forename: Göknur
authors.initials: G
authors.lastname: Terzi
authors.forename: T Onur
authors.initials: TO
authors.lastname: Kevenk
authors.forename: Mustafa
authors.initials: M
authors.lastname: Alişarli
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D007054:Ice Cream / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins; D012336:RNA, Ribosomal, 16S; C031818:enterotoxin B, staphylococcal