title: Dynamic behavior of rat phosphoenolpyruvate carboxykinase inhibitors: new mechanism for enzyme inhibition.
abstract: As an enzyme acting at the junction of gluconeogenic pathway, phosphoenolpyruvate carboxykinase (PEPCK) controls substrate flow from Krebs cycle toward glucose production. Therefore, it would be advantageous to design effective inhibitors to inactivate PEPCK in diabetes mellitus and other abnormalities caused by insulin resistance. Such inhibitors may compensate the metabolic consequences of ex-activity of PEPCK at these conditions. Understanding the mechanism by which inhibitors exert their effect on enzyme activity is of great interest for designing stronger inhibitors. In the present work, molecular dynamic simulations were used to study enzyme-inhibitor interactions. Our results indicate that inhibitors of PEPCK with their short chains interact with enzyme active site through non-covalent interactions of electrostatic and hydrogen bond nature. The data also show that inhibitors neither reach a stable state in their binding site nor make static complex with the enzyme active site. Instead, they interact with functional groups of active site residues in a dynamic fashion. In this way, oxalate and sulfoacetate carrying two negative groups of higher charge density and optimum spacing from each other, show more dynamic behavior (lower stability in their binding site) and more inhibitory effects than other inhibitors used (phosphonoformate, phosphoglycolate and 3-phosphonopropionate).
authors.affiliation: Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran. mrdayer@scu.ac.ir
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Dayer
authors.forename: Mohammad Saaid
authors.initials: MS
authors.lastname: Dayer
authors.forename: Omid
authors.initials: O
authors.lastname: Ghayour
mesh_terms: D000144:Acids, Acyclic / Q000378:metabolism; D000818:Animals; D020134:Catalytic Domain; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D051381:Rats
chemical_list: D000144:Acids, Acyclic; D004791:Enzyme Inhibitors; D010729:Phosphoenolpyruvate Carboxykinase (GTP)