title: A novel role of the N terminus of budding yeast histone H3 variant Cse4 in ubiquitin-mediated proteolysis.
abstract: Regulating levels of centromeric histone H3 (CenH3) variant is crucial for genome stability. Interaction of Psh1, an E3 ligase, with the C terminus of Cse4 has been shown to contribute to its proteolysis. Here, we demonstrate a role for ubiquitination of the N terminus of Cse4 in regulating Cse4 proteolysis for faithful chromosome segregation and a role for Doa1 in ubiquitination of Cse4.
authors.affiliation: Genetics Branch Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20889, USA.
authors.forename: Wei Chun
authors.initials: WC
authors.lastname: Au
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Dawson
authors.forename: David W
authors.initials: DW
authors.lastname: Rawson
authors.forename: Sara B
authors.initials: SB
authors.lastname: Taylor
authors.forename: Richard E
authors.initials: RE
authors.lastname: Baker
authors.forename: Munira A
authors.initials: MA
authors.lastname: Basrai
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C092746:CSE4 protein, S cerevisiae; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C099944:DOA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: Cse4; Doa1; Psh1; chromosome segregation; ubiquitin-mediated protein degradation