title: Determination of 3,4-dichlorinated biphenyl in soil samples by real-time immuno-PCR assay.
abstract: A real-time fluorescent quantitative immuno-polymerase chain reaction (RT-IPCR) assay was developed for the detection of non-dioxin-like polychlorinated biphenyl (PCB) congener in soil samples. Based on the construction of 3,4-dichlorinated biphenyl (IUPAC PCB12) hapten and its immunogen, the specific polyclonal antibodies (pAbs) to PCB12 was obtained and used to develop a direct competitive RT-IPCR assay. Using the optimized assay, a standard curve for PCB12 was prepared. The linear range for the determination of PCB12 was from 10.0 to 1.0 x 106 fg/mL with a correlation coefficient of 0.98 and a detection limit of 1.53 fg/mL. The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products. The results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry. This highly specific, sensitive, and robust assay can be applied to on-site tests of PCBs and serve as a model for other pollutant immunoassays.
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. chypds@126.com
authors.forename: Hanyu
authors.initials: H
authors.lastname: Chen
authors.forename: Huisheng
authors.initials: H
authors.lastname: Zhuang
mesh_terms: D000918:Antibody Specificity; D001360:Avidin; D007118:Immunoassay; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: C581581:3,4-dichlorinated biphenyl; D012989:Soil Pollutants; D001360:Avidin; D011078:Polychlorinated Biphenyls