title: [The effect of potential-dependent potassium uptake on membrane potential in rat brain mitochondria].
abstract: The effect of potential-dependent potassium uptake on the transmembrane potential difference (DeltaPsi(m)) in rat brain mitochondria has been studied. It was shown that in potassium concentration range of 0-120 mM the potential-dependent K(+)-uptake into matrix leads to the increase in respiration rate and mitochondrial depolarization. ATP-dependent potassium channel (K+(ATP)-channel) blockers, glibenclamide and 5-hydroxydecanoate, block approximately 35% of potential-dependent potassium uptake in the brain mitochondria. It was shown that K+(ATP)-channel blockage results in membrane repolarization by approximately 20% of control, which is consistent with experimental dependence of DeltaPsi(m) on the rate of potential-dependent potassium uptake. Obtained experimental data give the evidence that functional activity of K+(ATP)-channel is physiologically important in the regulation of membrane potential and energy-dependent processes in brain mitochondria.
authors.forename: O V
authors.initials: OV
authors.lastname: Akopova
authors.forename: V I
authors.initials: VI
authors.lastname: Nosar'
authors.forename: L I
authors.initials: LI
authors.lastname: Kolchinskaia
authors.forename: I N
authors.initials: IN
authors.lastname: Man'kovskaia
authors.forename: M K
authors.initials: MK
authors.lastname: Malysheva
authors.forename: V F
authors.initials: VF
authors.lastname: Sagach
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D003652:Decanoic Acids / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D006880:Hydroxy Acids / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D007700:Kinetics; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011188:Potassium / Q000378:metabolism* / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003470:Culture Media; D003652:Decanoic Acids; D006880:Hydroxy Acids; D026902:Potassium Channel Blockers; D015221:Potassium Channels; C052853:5-hydroxydecanoic acid; D000255:Adenosine Triphosphate; D011188:Potassium; D005905:Glyburide