title: Biomimetic construction of large engineered bone using hemoperfusion and cyto-capture in traumatic bone defect.
abstract: Due to lack of blood vessel systems, only a few tissues, such as skin, cartilage, and cornea, have been successfully constructed in vivo. Anticoagulative scaffolds have been used in drug-eluting stent systems both in animal studies and clinical therapies, as in the medicinal leech therapy used to salvage venous-congested microvascular free flaps improved perfusion inspired us to tackle this hurdle in bone tissue engineering. We hypothesize that a combination of bone marrow as the blood supply and a heparin/chitosan-coated acellular bone matrix that acts like hirudin, together with a vacuum-assisted closure therapy system, would provide blood perfusion to the scaffold. Using these methods, a biomimetically engineered bone construct would facilitate clinical translation in bone tissue engineering and offer new therapeutic strategies for reconstructing large bone defects if the hypothesis proves to be practical.
authors.affiliation: Department of Orthopedics, 89th Hospital of People's Liberation Army , Weifang, China .
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Shaofen
authors.initials: S
authors.lastname: Yu
authors.forename: Zhengguo
authors.initials: Z
authors.lastname: Wang
authors.forename: Xinjun
authors.initials: X
authors.lastname: Sun