title: [Comparison of selective oxidative stress parameters in the follicular fluid of infertile women and healthy fertile oocyte donors].
abstract: OBJECTIVE
  Follicular fluid (FF) affects oocyte development and disruption of its homeostasis has a crucial effect on egg developmental potential. The aim of this study was to compare the levels of selected oxidative stress markers in the FF of women with impaired fertility and healthy fertile oocytes donors.
  
  
  METHODS
  A retrospective comparative study.
  
  
  METHODS
  Faculty of Medicine in Pilsen, Charles University in Prague; Institute of Reproductive Medicine and Endocrinology, IVF Center Prof. Zech, Pilsen.
  
  
  METHODS
  Levels of homocysteine (Hcy), malondialdehyde (MDA), glutathione peroxidase (GPx), total antioxidant capacity (AOK) and total protein (CB) were analyzed in the FF. We have analysed FF of 146 women - 74 infertile patients (mean age 31 years, SD = 4.65) and 72 healthy fertile oocyte donors (mean age 26 years, SD = 4.44). Only blood free samples were studied after pooling of all FF samples each patient.
  
  
  RESULTS
  The study showed a statistically significantly higher Hcy levels (p < 0.0001) in the FF of healthy fertile women compared with impaired fertility group both - comparing the two groups regardless the age and in groups of the same age range (for the age group between 20 to 29 years isp = 0.0002, for the age group between 30 to 39 years is p < 0.0001). When divided into above age ranges we found statistically significantly higher levels of MDA in the control group aged 20 to 29 years compared to same age infertile patients (p = 0.0374) and statistically significantly higher AOK in infertile women between 30 to 39 years of age compared to same age control group (p = 0.0458).
  
  
  CONCLUSIONS
  The presence or on the contrary the absence of prooxidant parameters in the FF has an important role in the ability of conception and subsequent embryo development.
authors.affiliation: Ustav lekarske chemie a biochemie, LF UK, Plzen. vaclav.babuska@lfp.cuni.cz
authors.forename: V
authors.initials: V
authors.lastname: Babuška
authors.forename: M
authors.initials: M
authors.lastname: Cedíková
authors.forename: D
authors.initials: D
authors.lastname: Rajdl
authors.forename: J
authors.initials: J
authors.lastname: Racek
authors.forename: N H
authors.initials: NH
authors.lastname: Zech
authors.forename: L
authors.initials: L
authors.lastname: Trefil
authors.forename: A
authors.initials: A
authors.lastname: Mocková
authors.forename: Z
authors.initials: Z
authors.lastname: Ulčová-Gallová
authors.forename: Z
authors.initials: Z
authors.lastname: Novotný
authors.forename: M
authors.initials: M
authors.lastname: Králíčková
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006710:Homocysteine / Q000378:metabolism; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D014019:Tissue Donors
chemical_list: D000975:Antioxidants; D006710:Homocysteine; D008315:Malondialdehyde; D005979:Glutathione Peroxidase