title: Genetic heterogeneity of Campylobacter jejuni NCTC 11168 upon human infection.
abstract: Campylobacter jejuni NCTC 11168 variants before and after accidental human infection were sequenced with Illumina technology and mapped against the isogenic reference genome applying the Breseq pipeline. Only the frequencies of length variations of homopolymeric tracts in the contingency genes Cj0045c, Cj0456c, Cj1139c, Cj1145c, and Cj1306c and a deletion in Cj0184c were significantly different after human passage (p<0.01). Our results highlight differences in the selection of C. jejuni variants after human infection compared with those observed in animal models, emphasizing the genetic diversity of C. jejuni NCTC 11168 and the possible role of the host in the selection of bacterial determinants that might be involved in the adaptation and disease development.
authors.affiliation: Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. joana.revez@helsinki.fi
authors.forename: Joana
authors.initials: J
authors.lastname: Revez
authors.forename: Thomas
authors.initials: T
authors.lastname: Schott
authors.forename: Ann-Katrin
authors.initials: AK
authors.lastname: Llarena
authors.forename: Mirko
authors.initials: M
authors.lastname: Rossi
authors.forename: Marja-Liisa
authors.initials: ML
authors.lastname: Hänninen
mesh_terms: D000059:Accidents; D000818:Animals; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics*; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004781:Environmental Exposure; D006801:Humans; D051379:Mice; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D012692:Serial Passage
chemical_list: D004269:DNA, Bacterial