title: Quantitative analysis of organochlorine pesticides in human serum using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry.
abstract: Persistent organochlorine pesticides (OCPs) are well known to accumulate in environment and to instigate disruptions in endocrine system. In the present study, 18 kinds of OCPs were bio-monitored from 1904 adult serums. Analysis was performed with headspace gas chromatography (HS-GC) which has the advantage to require small volume of serum (1mL) without complex pretreatment and (13)C isotope labeled internal standard mix was used for quantitation. As a result, eleven out of 18 OCPs, including hexachlorobenzene (HCB) and dichlorodiphenyltrichloroethane metabolites (DDTs) were detected. HCB was the most frequently detected OCP (8.0%) with an average concentration of 1.74ngg(-1) lipid (2.10ngg(-1) lipid-99.80ngg(-1) lipid). p,p'-Dichlorodiphenyldichloroethylene (p,p'-DDE) had the highest average concentration of 21.65ngg(-1) lipid (12.80ngg(-1) lipid-2270ngg(-1) lipid) and was detected from 7.5% of serum samples. The average concentration of ∑DDT metabolites was 32.04ngg(-1) lipid (6.61ngg(-1) lipid-2800ngg(-1) lipid) with detection frequency of 9.0%. Results interpreted with respect to age groups showed increase in ∑OCPs and ∑DDTs concentrations as age increases, except for the 20. Results interpreted with respect to gender, in general, illustrated higher detected concentration of OCPs in male serums than in female serums; however, the result trend was reversed in age group of the sixties. The above results will be applicable to health impact assessment of Koreans in relation to environmental contaminations.
authors.affiliation: Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Miok
authors.initials: M
authors.lastname: Kim
authors.forename: Na Rae
authors.initials: NR
authors.lastname: Song
authors.forename: Jongki
authors.initials: J
authors.lastname: Hong
authors.forename: Jeongae
authors.initials: J
authors.lastname: Lee
authors.forename: Heesoo
authors.initials: H
authors.lastname: Pyo
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000097:blood* / Q000302:isolation & purification*; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D010575:Pesticides / Q000097:blood* / Q000302:isolation & purification*; D012737:Sex Factors; D052617:Solid Phase Microextraction; D055815:Young Adult
chemical_list: D006843:Hydrocarbons, Chlorinated; D010575:Pesticides