title: Thermostable luciferase from Luciola cruciate for imaging of carbon nanotubes and carbon nanotubes carrying doxorubicin using in vivo imaging system.
abstract: In the present study, we introduce a novel method for in vivo imaging of the biodistribution of single wall carbon nanotubes (SWNTs) labeled with recombinant thermo-stable Luciola cruciata luciferase (LcL). In addition, we highlight a new application for green fluorescent proteins in which they are utilized as imaging moieties for SWNTs. Carbon nanotubes show great positive potential compared to other drug nanocarriers with respect to loading capacity, cell internalization, and biodegradability. We have also studied the effect of binding mode (chemical conjugation and physical adsorption) on the chemiluminescence activity, decay rate, and half-life. We have shown that through proper chemical conjugation of LcL to CNTs, LcL remained biologically active for the catalysis of d-luciferin in the presence of ATP to release detectable amounts of photons for in vivo imaging. Chemiluminescence of LcL allows imaging of CNTs and their cargo in nonsuperficial locations at an organ resolution with no need of an excitation source. Loading LcL-CNTs with the antitumor antibiotic doxorubicin did not alter their biological activity for imaging. In vivo imaging of LcL-CNTs has been carried out using "IVIS spectrum" showing the uptake of LcL-CNTs by different organs in mice. We believe that the LcL-CNT system is an advanced powerful tool for in vivo imaging and therefore a step toward the advancement of the nanomedicine field.
authors.affiliation: Experimental Cancer Medicine, KFC, Novum, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Ramy
authors.initials: R
authors.lastname: El-Sayed
authors.forename: Mohamed
authors.initials: M
authors.lastname: Eita
authors.forename: Asa
authors.initials: A
authors.lastname: Barrefelt
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
authors.forename: Himanshu
authors.initials: H
authors.lastname: Jain
authors.forename: Mona
authors.initials: M
authors.lastname: Fares
authors.forename: Arne
authors.initials: A
authors.lastname: Lundin
authors.forename: Mikael
authors.initials: M
authors.lastname: Crona
authors.forename: Khalid
authors.initials: K
authors.lastname: Abu-Salah
authors.forename: Mamoun
authors.initials: M
authors.lastname: Muhammed
authors.forename: Moustapha
authors.initials: M
authors.lastname: Hassan
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging; D004317:Doxorubicin / Q000737:chemistry*; D004795:Enzyme Stability; D048888:Fireflies / Q000201:enzymology; D008156:Luciferases / Q000737:chemistry*; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013194:Staining and Labeling; D013696:Temperature
chemical_list: D037742:Nanotubes, Carbon; D004317:Doxorubicin; D008156:Luciferases