title: Detection of orexin A neuropeptide in biological fluids using a zinc oxide field effect transistor.
abstract: Biomarkers which are indicative of acute physiological and emotional states are studied in a number of different areas in cognitive neuroscience. Currently, many cognitive studies are conducted based on programmed tasks followed by timed biofluid sampling, central laboratory processing, and followed by data analysis. In this work, we present a sensor platform capable of rapid biomarker detection specific for detecting neuropeptide orexin A, found in blood and saliva and known as an indicator of fatigue and cognitive performance. A peptide recognition element that selectively binds to orexin A was designed, characterized, and functionalized onto a zinc oxide field effect transistor to enable rapid detection. The detection limit using the sensor platform was sub-picomolar in water, and picomolar to nanomolar levels in saliva and serum. The transistor and recognition element sensor platform can be easily expanded, allowing for multiple biomarkers to be detected simultaneously, lending itself to complex biomarker analysis applicable to rapid feedback for neuroscience research and physiological monitoring.
authors.affiliation: 711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratories, Wright Patterson Air Force Base, Dayton, OH 45433, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Hagen
authors.forename: Wanda
authors.initials: W
authors.lastname: Lyon
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Chushak
authors.forename: Melanie
authors.initials: M
authors.lastname: Tomczak
authors.forename: Rajesh
authors.initials: R
authors.lastname: Naik
authors.forename: Morley
authors.initials: M
authors.lastname: Stone
authors.forename: Nancy
authors.initials: N
authors.lastname: Kelley-Loughnane
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000378:metabolism; D000068797:Orexins; D051381:Rats; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D044967:Serum / Q000737:chemistry* / Q000378:metabolism; D014173:Transistors, Electronic; D015034:Zinc Oxide / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C000596229:HCRT protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D015034:Zinc Oxide