title: MicroRNA-365 regulates NKX2-1, a key mediator of lung cancer.
abstract: MicroRNAs constitute a class of small noncoding RNAs that play roles in tumorigenesis. We found that NKX2-1 protein levels were generally high in the lung cancer tissues whereas miRNA-365 expression levels were downregulated. Ectopic miR-365 expression decreased NKX2-1 expression in lung cancer cell lines. Transfection of a miR-365 mimic led to reduced proliferation of lung cancer cells; conversely, a miR-365 inhibitor slightly increased cell proliferation. The NKX2-1 overexpression significantly increased the cell proliferation by overcoming the suppressive effect of miR-365. Our data suggest that miR-365 is an important regulator of NKX2-1 and can be a target for lung cancer therapies.
authors.affiliation: Department of Oral Microbiology, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Kang
authors.forename: Heon-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Je-Yoel
authors.initials: JY
authors.lastname: Cho
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C546819:MIRN365 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors