title: Ultrasound-sensitive siRNA-loaded nanobubbles formed by hetero-assembly of polymeric micelles and liposomes and their therapeutic effect in gliomas.
abstract: Ultrasound (US)-sensitive nanobubble (NB) which may utilize the physical power of US exposure to improve delivery efficiency to target cells is emerging as one of the most promising nanocarriers for drug delivery. On the basis of successfully fabricating NBs with the ability of passively accumulating in tumor tissue, in this study we synthesized a US-sensitive NB bearing siRNA (siRNA-NB) for tumor therapy via a hetero-assembling strategy using the siRNA-complexed polymeric micelles and gas-cored liposomes. The US exposure-aided siRNA transfection effectively enhanced the gene silencing effect of siRNA-NBs both in vitro and in vivo, which resulted in much elevated level of cancer cell apoptosis. Consequently, significantly improved therapeutic effect was achieved in a nude mouse glioma model, using siRNA-NBs bearing siRNA to target the anti-apoptosis gene sirtuin 2 (SIRT2). These results show that, with the aid of US exposure, the US-sensitive siRNA-NB may be an ideal delivery vector to mediate highly effective RNA interference for tumor treatment.
authors.affiliation: Department of Medical Ultrasonic, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Tinghui
authors.initials: T
authors.lastname: Yin
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Jingguo
authors.initials: J
authors.lastname: Li
authors.forename: Rongqin
authors.initials: R
authors.lastname: Zheng
authors.forename: Bowen
authors.initials: B
authors.lastname: Zheng
authors.forename: Du
authors.initials: D
authors.lastname: Cheng
authors.forename: Ruitang
authors.initials: R
authors.lastname: Li
authors.forename: Jieyi
authors.initials: J
authors.lastname: Lai
authors.forename: Xintao
authors.initials: X
authors.lastname: Shuai
mesh_terms: D000818:Animals; D017209:Apoptosis; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D005910:Glioma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D045423:Microbubbles; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000378:metabolism*; D051381:Rats; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014465:Ultrasonics; D023041:Xenograft Model Antitumor Assays
chemical_list: D008081:Liposomes; D008823:Micelles; D011108:Polymers; D034741:RNA, Small Interfering; C097475:Sirt2 protein, rat; D056565:Sirtuin 2