title: Quantum chemistry as a tool in asymmetric biocatalysis: limonene epoxide hydrolase test case.
abstract: Cluster model: Large active-site models (see figure) are used to investigate the selectivity of limonene epoxide hydrolase, both the wild type and mutants optimized through directed evolution. Good agreement is found between theory and the experimental data, thus demonstrating that the quantum chemical cluster approach can be a powerful tool in the field of asymmetric biocatalysis.
authors.affiliation: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, 10691 Stockholm, Sweden.
authors.forename: Maria E S
authors.initials: ME
authors.lastname: Lind
authors.forename: Fahmi
authors.initials: F
authors.lastname: Himo
mesh_terms: D055162:Biocatalysis; D020134:Catalytic Domain; D053138:Cyclohexenes / Q000737:chemistry*; D004851:Epoxide Hydrolases / Q000737:chemistry*; D000077222:Limonene; D008958:Models, Molecular; D011789:Quantum Theory; D013237:Stereoisomerism; D013729:Terpenes / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D053138:Cyclohexenes; D013729:Terpenes; D000077222:Limonene; D004851:Epoxide Hydrolases