title: Kinetics of transesterification of olive oil with methanol catalyzed by immobilized lipase derived from an isolated Burkholderia sp. strain.
abstract: This work was carried out to investigate the acyl migration phenomena which has been considered as the factor having significant impact on kinetics of transesterification of oils catalyzed by a Burkholderia lipase with 1,3-regioselectivity. Transesterification of olive oil with methanol catalyzed by the immobilized lipase produces various intermediates, including 1-monoglyceride, 2-monoglyceride, 1,2-diglyceride, and 1,3-diglyceride. Migration kinetics of fatty acid groups from sn-2 of 2-monoglyceride and 1,2-diglyceride to 1-monoglyceride and 1,3-diglyceride were investigated for the temperature range of 25-65°C. The kinetics of transesterification of olive oil with methanol involving acyl migration in the presence of water was also systematically studied at 25, 40, and 65°C. Increasing temperature could increase the acyl migration rate. The overall biodiesel conversion was improved from 73.4% (at 25°C) to 90.0% and 92.4% when conducting at 40 and 65°C, respectively. Thermodynamics aspects of equilibrium state of the immobilized lipase-catalyzed transesterification were also discussed.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Dang-Thuan
authors.initials: DT
authors.lastname: Tran
authors.forename: Yi-Jan
authors.initials: YJ
authors.lastname: Lin
authors.forename: Ching-Lung
authors.initials: CL
authors.lastname: Chen
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D056804:Biofuels; D019117:Burkholderia / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D058185:Magnetite Nanoparticles; D000432:Methanol / Q000378:metabolism*; D008956:Models, Chemical; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D005227:Fatty Acids; D005989:Glycerides; D058185:Magnetite Nanoparticles; D000069463:Olive Oil; D010938:Plant Oils; D008049:Lipase; D000432:Methanol
keywords: Acyl migration; Burkholderia sp; Immobilized lipase; Olive oil; Transesterification kinetics