title: LIN-12/Notch signaling instructs postsynaptic muscle arm development by regulating UNC-40/DCC and MADD-2 in Caenorhabditis elegans.
abstract: The diverse cell types and the precise synaptic connectivity between them are the cardinal features of the nervous system. Little is known about how cell fate diversification is linked to synaptic target choices. Here we investigate how presynaptic neurons select one type of muscles, vm2, as a synaptic target and form synapses on its dendritic spine-like muscle arms. We found that the Notch-Delta pathway was required to distinguish target from non-target muscles. APX-1/Delta acts in surrounding cells including the non-target vm1 to activate LIN-12/Notch in the target vm2. LIN-12 functions cell-autonomously to up-regulate the expression of UNC-40/DCC and MADD-2 in vm2, which in turn function together to promote muscle arm formation and guidance. Ectopic expression of UNC-40/DCC in non-target vm1 muscle is sufficient to induce muscle arm extension from these cells. Therefore, the LIN-12/Notch signaling specifies target selection by selectively up-regulating guidance molecules and forming muscle arms in target cells. DOI:http://dx.doi.org/10.7554/eLife.00378.001.
authors.affiliation: Department of Biology , Howard Hughes Medical Institute, Stanford University , Stanford , United States.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Li
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Collins
authors.forename: Michael R
authors.initials: MR
authors.lastname: Koelle
authors.forename: Kang
authors.initials: K
authors.lastname: Shen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005838:Genotype; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis; D009119:Muscle Contraction; D009132:Muscles / Q000294:innervation*; D009154:Mutation; D055495:Neurogenesis; D010058:Oviposition; D019899:Paracrine Communication; D010641:Phenotype; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D015222:Sodium Channels / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*; D014844:Vulva / Q000294:innervation*
chemical_list: D029742:Caenorhabditis elegans Proteins; D015815:Cell Adhesion Molecules; D047908:Intracellular Signaling Peptides and Proteins; C082460:Lin-12 protein, C elegans; D051880:Receptors, Notch; D015222:Sodium Channels; C551653:TRIM-9 protein, C elegans; C102174:UNC-40 protein, C elegans; C489774:apx-1 protein, C elegans
keywords: C. elegans; Notch/LIN-12; UNC-40/DCC; muscle arm; postsynaptic development
references.citation: PLoS One. 2009;4(3):e4625
references.pmid: 19259264
references.citation: Nature. 2009 Jun 11;459(7248):842-6
references.pmid: 19421194
references.citation: Cell. 2002 Sep 6;110(5):649-60
references.pmid: 12230981
references.citation: Nucleic Acids Res. 2007;35(14):4583-96
references.pmid: 17586813
references.citation: Biochem Biophys Res Commun. 2000 Aug 28;275(2):652-60
references.pmid: 10964718
references.citation: Nat Rev Genet. 2000 Oct;1(1):20-9
references.pmid: 11262869
references.citation: Annu Rev Cell Dev Biol. 2011;27:697-729
references.pmid: 21740233
references.citation: Dev Dyn. 2011 Feb;240(2):324-32
references.pmid: 21246649
references.citation: Nature. 1995 Sep 28;377(6547):351-4
references.pmid: 7566091
references.citation: Development. 2009 Mar;136(6):911-22
references.pmid: 19211675
references.citation: Dev Biol. 2008 Jan 1;313(1):256-66
references.pmid: 18036582
references.citation: Genetics. 1994 Aug;137(4):987-97
references.pmid: 7982579
references.citation: Neuron. 1999 Mar;22(3):451-61
references.pmid: 10197526
references.citation: Neuron. 2010 Oct 6;68(1):9-18
references.pmid: 20920787
references.citation: Dev Cell. 2004 Feb;6(2):183-92
references.pmid: 14960273
references.citation: Neuron. 1990 Jan;4(1):61-85
references.pmid: 2310575
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3263-8
references.pmid: 10077672
references.citation: Nature. 1988 Oct 6;335(6190):551-4
references.pmid: 3419532
references.citation: Dev Biol. 2002 Nov 1;251(1):178-92
references.pmid: 12413907
references.citation: Cell. 1996 Oct 18;87(2):175-85
references.pmid: 8861902
references.citation: Science. 2007 Oct 5;318(5847):103-6
references.pmid: 17916735
references.citation: Genes Dev. 2000 Aug 15;14(16):2003-14
references.pmid: 10950865
references.citation: Mech Dev. 1995 Sep;53(1):73-85
references.pmid: 8555113
references.citation: Annu Rev Cell Dev Biol. 2009;25:161-95
references.pmid: 19575668
references.citation: Genetics. 1993 Nov;135(3):755-63
references.pmid: 8293977
references.citation: Development. 1996 May;122(5):1373-83
references.pmid: 8625826
references.citation: Genes Dev. 1998 Aug 15;12(16):2623-35
references.pmid: 9716413
references.citation: Development. 2005 Jul;132(13):3079-92
references.pmid: 15930100
references.citation: Curr Biol. 2005 Aug 9;15(15):1413-9
references.pmid: 16085495
references.citation: Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19389-94
references.pmid: 22084112
references.citation: Neuroscience. 1999;93(2):433-9
references.pmid: 10465425
references.citation: Cell. 1998 May 15;93(4):519-29
references.pmid: 9604928
references.citation: Science. 1999 Oct 22;286(5440):741-6
references.pmid: 10531053
references.citation: Development. 1998 Jul;125(13):2479-88
references.pmid: 9609831
references.citation: Development. 2003 Aug;130(16):3757-66
references.pmid: 12835392
references.citation: Neuron. 1998 Apr;20(4):667-81
references.pmid: 9581760
references.citation: Neuron. 2011 Feb 10;69(3):437-44
references.pmid: 21315255
references.citation: EMBO J. 1994 Apr 15;13(8):1799-805
references.pmid: 7909512
references.citation: Nature. 2011 Feb 10;470(7333):259-63
references.pmid: 21270798
references.citation: Development. 2010 Nov;137(21):3719-27
references.pmid: 20940230
references.citation: WormBook. 2005 Aug 08;:1-16
references.pmid: 18050403
references.citation: Cell. 1983 Sep;34(2):435-44
references.pmid: 6616618
references.citation: J Neurogenet. 2009;23(4):395-404
references.pmid: 19863270
references.citation: Nat Cell Biol. 2001 Nov;3(11):983-91
references.pmid: 11715019
references.citation: Genetics. 2012 Jul;191(3):655-69
references.pmid: 22785620
references.citation: Nat Neurosci. 2007 Feb;10(2):153-60
references.pmid: 17220884
references.citation: Annu Rev Neurosci. 2003;26:565-97
references.pmid: 12730322
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15787-91
references.pmid: 9861048
references.citation: Neuron. 2000 Mar;25(3):549-61
references.pmid: 10774724
references.citation: Development. 2011 Sep;138(17):3593-612
references.pmid: 21828089
references.citation: Cell. 1996 Oct 18;87(2):187-95
references.pmid: 8861903
references.citation: Cell. 1985 Dec;43(3 Pt 2):583-90
references.pmid: 3000611
references.citation: Cell. 1994 Aug 12;78(3):409-24
references.pmid: 8062384
references.citation: Nature. 2002 Dec 12;420(6916):686-90
references.pmid: 12478297
references.citation: Cell. 1993 Jul 16;74(1):43-55
references.pmid: 8101475
references.citation: Nat Genet. 2008 Nov;40(11):1375-83
references.pmid: 18953339
references.citation: Annu Rev Neurosci. 2002;25:251-81
references.pmid: 12052910
references.citation: Genetics. 2001 Apr;157(4):1599-610
references.pmid: 11290716
references.citation: Am J Physiol Cell Physiol. 2001 Jun;280(6):C1616-22
references.pmid: 11350757
references.citation: Nat Neurosci. 2000 Jan;3(1):30-40
references.pmid: 10607392
references.citation: Dev Cell. 2010 Jun 15;18(6):950-60
references.pmid: 20627077
references.citation: Development. 1997 Oct;124(20):4133-41
references.pmid: 9374409
references.citation: Philos Trans R Soc Lond B Biol Sci. 1986 Nov 12;314(1165):1-340
references.pmid: 22462104
references.citation: Nature. 2008 Jan 24;451(7177):465-9
references.pmid: 18216854
references.citation: Cell. 1989 Jun 30;57(7):1237-45
references.pmid: 2736627
references.citation: J Neurosci. 2001 Mar 15;21(6):2001-14
references.pmid: 11245684
references.citation: Cell. 1995 Aug 25;82(4):631-41
references.pmid: 7664342
references.citation: Curr Opin Genet Dev. 2012 Aug;22(4):339-46
references.pmid: 22608692
references.citation: Nat Rev Neurosci. 2006 Feb;7(2):93-102
references.pmid: 16429119
references.citation: PLoS Biol. 2008 Aug 5;6(8):e198
references.pmid: 18687026
references.citation: Curr Biol. 2004 Apr 20;14(8):R311-3
references.pmid: 15084301
references.citation: Cell. 1985 Dec;43(3 Pt 2):567-81
references.pmid: 3935325
references.citation: Dev Cell. 2010 Jun 15;18(6):961-72
references.pmid: 20627078
references.citation: Genes Dev. 1996 Jun 1;10(11):1395-410
references.pmid: 8647436
references.citation: Nat Neurosci. 2011 Dec 04;15(1):57-63
references.pmid: 22138642
references.citation: J Biol Chem. 1999 Dec 17;274(51):36801-7
references.pmid: 10593990
references.citation: Development. 2004 Jun;131(11):2595-604
references.pmid: 15115754
references.citation: Dev Biol. 1999 Dec 1;216(1):114-34
references.pmid: 10588867
references.citation: Cell. 2004 Mar 19;116(6):869-81
references.pmid: 15035988
references.citation: Nat Neurosci. 2009 Dec;12(12):1542-50
references.pmid: 19915565
references.citation: Nat Neurosci. 2011 Feb;14(2):165-72
references.pmid: 21186357
references.citation: Neuron. 1998 Jul;21(1):203-14
references.pmid: 9697864
references.citation: Biochim Biophys Acta. 2007 Jan;1769(1):5-19
references.pmid: 17258327
references.citation: Nature. 2012 Mar 18;484(7393):201-7
references.pmid: 22425994
references.citation: Cell. 2007 Jan 26;128(2):399-410
references.pmid: 17254975
references.citation: Cell. 1994 Dec 30;79(7):1187-98
references.pmid: 8001154
references.citation: Cell. 2003 Mar 7;112(5):619-30
references.pmid: 12628183
references.citation: J Neurosci. 2013 Jan 9;33(2):761-75
references.pmid: 23303953
references.citation: Curr Opin Neurobiol. 2011 Feb;21(1):93-9
references.pmid: 21087855
references.citation: Nature. 1992 Aug 6;358(6386):470-6
references.pmid: 1641037
references.citation: Genes Dev. 1998 Jun 15;12(12):1751-62
references.pmid: 9637676
references.citation: Curr Opin Cell Biol. 2003 Oct;15(5):621-32
references.pmid: 14519398