title: Specific binding of paraprotein to platelet receptors as a cause of platelet dysfunction in monoclonal gammopathies.
abstract: The study included 48 untreated patients with monoclonal gammopathies (MG). Paraprotein was isolated from the serum of 10 patients with decreased platelet aggregation. Platelet aggregation was measured before and after the addition of the isolated paraprotein to platelet-rich plasma (PRP) from 10 healthy donors, in vitro. Expression of platelet von Willebrand factor (vWF) receptor glycoprotein (GP)Ib and platelet collagen receptor GPVI was determined by flow cytometry in the PRP of healthy donors before and after the addition of isolated paraprotein using the monoclonal antibodies, CD42b (for GPIb) and CD36 (for GPVI). Flowcytometry showed that expression of CD42b and CD36 positive cells was reduced after the addition of isolated paraprotein to PRP from healthy donors (p < 0.001). These investigations demonstrated that paraprotein causes platelet dysfunction in patients with MG due to specific binding to the platelet vWF receptor GPIb and platelet collagen receptor GPVI.
authors.affiliation: Clinic for Hematology, Clinical Center of Serbia, Belgrade, Serbia.
authors.forename: I
authors.initials: I
authors.lastname: Djunic
authors.forename: I
authors.initials: I
authors.lastname: Elezovic
authors.forename: M
authors.initials: M
authors.lastname: Vucic
authors.forename: T
authors.initials: T
authors.lastname: Srdic-Rajic
authors.forename: A
authors.initials: A
authors.lastname: Konic-Ristic
authors.forename: V
authors.initials: V
authors.lastname: Ilic
authors.forename: N
authors.initials: N
authors.lastname: Milic
authors.forename: Jelena
authors.initials: J
authors.lastname: Bila
authors.forename: Nada
authors.initials: N
authors.lastname: Suvajdzic-Vukovic
authors.forename: Marijana
authors.initials: M
authors.lastname: Virijevic
authors.forename: Darko
authors.initials: D
authors.lastname: Antic
authors.forename: Ana
authors.initials: A
authors.lastname: Vidovic
authors.forename: D
authors.initials: D
authors.lastname: Tomin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000378:metabolism*; D018955:CD36 Antigens / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010265:Paraproteinemias / Q000175:diagnosis* / Q000378:metabolism; D010266:Paraproteins / Q000378:metabolism*; D010974:Platelet Aggregation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D053657:Platelet-Rich Plasma / Q000378:metabolism; D011485:Protein Binding
chemical_list: D018955:CD36 Antigens; D010266:Paraproteins; D019038:Platelet Glycoprotein GPIb-IX Complex