title: Electrochemical removal of metallic implants from Technovit 9100 New embedded hard and soft tissues prior to histological sectioning.
abstract: Solid metallic implants in soft or hard tissues are serious challenges for histological processing. However, metallic implants are more frequently used in e.g. cardiovascular or orthopaedic therapies. Before clinical use, these devices need to be tested thoroughly in a biological environment and histological analysis of their biocompatibility is a major requirement. To allow the histological analysis of metallic implants in tissues especially in calcified hard tissues, we describe a method for embedding these tissues in the resin Technovit 9100 New and removing the metallic implants by electrochemical dissolution. With the combination of these two processes, we are able to achieve 5 μm thick sections from soft or hard tissues with a superior preservation of tissue architecture and especially the implant-tissue interface. These sections can be stained by classical stainings, immunohistochemical and enzymehistochemical as well as DNA-based staining methods.
authors.affiliation: Laboratory for Biomechanics and Biomaterials, Department of Orthopaedic Surgery, Hannover Medical School, Anna-von-Borries-Straße 1-7, 30625, Hannover, Germany.
authors.forename: Elmar
authors.initials: E
authors.lastname: Willbold
authors.forename: Mattias
authors.initials: M
authors.lastname: Reebmann
authors.forename: Richard
authors.initials: R
authors.lastname: Jeffries
authors.forename: Frank
authors.initials: F
authors.lastname: Witte
mesh_terms: D000818:Animals; D055664:Electrochemical Techniques / Q000295:instrumentation; D005269:Femur / Q000166:cytology*; D006615:Hip; D016591:Histocytological Preparation Techniques / Q000295:instrumentation; D006801:Humans; D051379:Mice; D019736:Prostheses and Implants; D011817:Rabbits; D012867:Skin / Q000166:cytology*; D015607:Stents; D013552:Swine
references.citation: Biotech Histochem. 1999 Jan;74(1):40-8
references.pmid: 10190260
references.citation: Eur Cell Mater. 2003 Dec 31;6:57-71; discussion 71
references.pmid: 14722903
references.citation: J Biomed Mater Res A. 2007 Jun 1;81(3):748-56
references.pmid: 17390368
references.citation: Semin Immunopathol. 2011 May;33(3):221-33
references.pmid: 21271251
references.citation: Acta Biomater. 2010 Nov;6(11):4447-55
references.pmid: 20601246
references.citation: Biomaterials. 1990 Oct;11(8):596-601
references.pmid: 2279062
references.citation: J Biomed Mater Res. 1979 Mar;13(2):337-8
references.pmid: 372195
references.citation: J Biomed Mater Res. 1995 Aug;29(8):987-92
references.pmid: 7593042
references.citation: Microsc Res Tech. 2010 Aug;73(8):741-5
references.pmid: 19953665
references.citation: Biomaterials. 2008 Jul;29(20):2941-53
references.pmid: 18440630
references.citation: Acta Biomater. 2010 May;6(5):1861-8
references.pmid: 20035905
references.citation: J Oral Pathol. 1982 Aug;11(4):318-26
references.pmid: 6809919
references.citation: Microsc Res Tech. 2009 Jul;72(7):501-6
references.pmid: 19301267
references.citation: J Physiol Pharmacol. 2009 Dec;60 Suppl 8:43-9
references.pmid: 20400791