title: Interaction of the iron(II) cage complexes with proteins: protein fluorescence quenching study.
abstract: Interaction of the iron(II) mono- and bis-clathrochelates with bovine serum albumin (BSA), β-lactoglobulin, lysozyme and insulin was studied by the steady-state and time-resolved fluorescent spectroscopies. These cage complexes do not make significant impact on fluorescent properties of β-lactoglobulin, lysozyme and insulin. At the same time, the monoclathrochelates strongly quench a fluorescence intensity of BSA and substantially decrease its excited state lifetime due to their binding to this protein. This occurs due to the excitation energy transfer from a tryptophan residue to a cage molecule or/and to the change of the tryptophan nearest environment caused by either clathrochelate binding or an alteration of the BSA conformation. The effect of the iron(II) bis-clathrochelate on BSA fluorescence is much weaker as compared to its monomacrobicyclic analogs as a result of an increase in its size.
authors.affiliation: Institute of Molecular Biology and Genetics NASU, Kyiv, Ukraine. mlosytskyy@gmail.com
authors.forename: Mykhaylo Y
authors.initials: MY
authors.lastname: Losytskyy
authors.forename: Vladyslava B
authors.initials: VB
authors.lastname: Kovalska
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Varzatskii
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Sergeev
authors.forename: Sergiy M
authors.initials: SM
authors.lastname: Yarmoluk
authors.forename: Yan Z
authors.initials: YZ
authors.lastname: Voloshin
mesh_terms: D000818:Animals; D002417:Cattle; D005296:Ferrous Compounds / Q000737:chemistry*; D005453:Fluorescence; D007328:Insulin / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D008968:Molecular Conformation; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005296:Ferrous Compounds; D007328:Insulin; D007782:Lactoglobulins; D012710:Serum Albumin, Bovine; D009113:Muramidase
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