title: The effect of curcumin on sepsis-induced acute lung injury in a rat model through the inhibition of the TGF-β1/SMAD3 pathway.
abstract: Curcumin has the potential to treat inflammatory diseases. This study investigated its effect on sepsis-induced acute lung injury (ALI) in a rat model. 125 healthy rats were randomly divided into five groups, including normal group, sham-operated group, sepsis group, dimethyl sulfoxide group, and curcumin-treated group (25 rats in each subgroup). Sepsis-induced acute lung injury was affected by cecal ligation and puncture surgery. At 0, 6, 12, 24, and 48 h after treatment, the lungs were harvested for histological and protein expression examinations. 24h after the initial treatment, real-time PCR and Western blot analysis showed that the expression of TGF-β1 and SMAD3-dependent signaling pathway was significantly decreased in the curcumin-treated group than other control groups (P<0.05). Therefore, curcumin played a protective role in sepsis-induced ALI, possibly through the inhibition of the expression of TGF-β1/SMAD3 pathway which may provide a new strategy for the treatment of sepsis-induced ALI.
authors.affiliation: Department of Emergency, the First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Shi-hui
authors.initials: SH
authors.lastname: Lin
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.forename: Ju
authors.initials: J
authors.lastname: Cao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003474:Curcumin / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000150:complications / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D051900:Smad3 Protein / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D051900:Smad3 Protein; C497787:Smad3 protein, rat; D053773:Transforming Growth Factor beta1; D003474:Curcumin