title: In vitro anti-propionibacterium activity by curcumin containing vesicle system.
abstract: Propionibacterium acnes acts a critical role in the development of inflammatory acne when it overgrows in pilosebaceous units. The spread of multiple drug resistance bacteria indicates a growing need for new antimicrobial agents. The objective of this study is to develop lipid vehicles to deliver curcumin and inhibit P. acnes in the skin. The inhibitory activities of the curcumin containing vehicles against P. acnes were studied by the bioluminescence assay. Curcumin accumulation patterns in neonate pig skin were studied using Franz diffusion cells and confocal laser scanning microscopy. The physicochemical properties of the curcumin containing vehicles including interfacial tension, size distribution and viscosity were analyzed. Significant curcumin accumulation (362±8 µg/g skin) was observed with the lipid vehicles developed herein. Curcumin (0.43 µg/mL) in the vehicles significantly inhibited the growth of P. acnes. Confocal laser scanning microscopy confirmed the formation of curcumin reservoir in the skin by the curcumin-loaded vehicles. Curcumin-loaded vehicles could efficiently accumulate in the skin and inhibit P. acnes in vitro. Our results highlight the potential of using vehicles containing lauric acid and curcumin as an alternative treatment for acne vulgaris.
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan, Taiwan 333, R.O.C. chl@mail.cgu.edu.tw
authors.forename: Chi-Hsien
authors.initials: CH
authors.lastname: Liu
authors.forename: Hsin-Ying
authors.initials: HY
authors.lastname: Huang
mesh_terms: D000152:Acne Vulgaris / Q000188:drug therapy; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D003474:Curcumin / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D007850:Lauric Acids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D011425:Propionibacterium acnes / Q000187:drug effects; D012867:Skin / Q000382:microbiology / Q000473:pathology; D012869:Skin Absorption; D013500:Surface Tension; D013552:Swine; D014783:Viscosity
chemical_list: D000900:Anti-Bacterial Agents; D007850:Lauric Acids; D008081:Liposomes; C030358:lauric acid; D003474:Curcumin