title: High incidence of lipid deposition in the liver of rats fed a diet supplemented with branched-chain amino acids under vitamin B6 deficiency.
abstract: Male Wistar rats were fed four diets composed of purified 20% vitamin-free casein diet with (+) or without (-) vitamin B(6) (7.0 mg of pyridoxine HCl/kg of diet) and with (+) or without (-) branched-chain amino acids (BCAAs) of valine, leucine, and isoleucine (4.75%): B(6)(+)BCAA(-); B(6)(+)BCAA(+); B(6)(-)BCAA(-); and B(6)(-)BCAA(+) for 21 d. Among rats fed the B(6)(-)BCAA(+) diet, about a half showed lipid deposition in the liver. On the other hand, serum triacylglycerol levels in the B(6)(-)BCAA(+) group tended to be decreased. Hepatic triacylglycerol and cholesterol levels tended to increase in the B(6)(-)BCAA(+) group compared with the other three groups. Serum apolipoprotein B and apolipoprotein E (apo E) levels in the B(6)(-)BCAA(+) group were the lowest among the three groups. In contrast, hepatic apo E levels in the B(6)(-)BCAA(+) group were the highest among the three groups. High-performance liquid chromatography of pooled serum of rats with lipid deposits revealed that triacylglycerol and cholesterol levels in very low-density lipoprotein (VLDL) were decreased compared with other diet groups. These results strongly suggest that one of the mechanisms of lipid deposition in rats fed a B(6)(-)BCAA(+) diet is due to impaired secretion of VLDL.
authors.affiliation: Department of Living Science, Shikoku University, Tokushima, Tokushima, Japan. tae-kaimoto@shikoku-u.ac.jp
authors.forename: Tae
authors.initials: T
authors.lastname: Kaimoto
authors.forename: Mayumi
authors.initials: M
authors.lastname: Shibuya
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Hideo
authors.initials: H
authors.lastname: Maeda
mesh_terms: D000597:Amino Acids, Branched-Chain / Q000009:adverse effects* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001055:Apolipoproteins B / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood / Q000378:metabolism; D002364:Caseins / Q000008:administration & dosage; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D015243:Cholesterol, VLDL / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D019587:Dietary Supplements; D005234:Fatty Liver / Q000209:etiology* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011736:Pyridoxine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood / Q000378:metabolism; D026681:Vitamin B 6 Deficiency / Q000150:complications* / Q000378:metabolism; D014803:Vitamin B Complex / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000597:Amino Acids, Branched-Chain; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D002364:Caseins; D015243:Cholesterol, VLDL; D014280:Triglycerides; D014803:Vitamin B Complex; D002784:Cholesterol; D011736:Pyridoxine