title: Structure of Neisseria meningitidis lipoprotein GNA1162.
abstract: GNA1162, a predicted lipoprotein from Neisseria meningitidis, is a potential candidate for a universal vaccine against meningococcal disease caused by N. meningitidis serogroup B. Here, the crystal structure of GNA1162 at 1.89 Å resolution determined by single-wavelength anomalous dispersion (SAD) is reported. The structure of GNA1162 appears to be a dimer in the crystallographic asymmetric unit as well as in solution. The overall structure of the dimer indicates that each monomer inserts its C-terminal α5 helix into the hydrophobic groove of the other molecule. Moreover, the β4 strands of each monomer lie antiparallel to each other and interact through multiple main-chain hydrogen bonds. Through structural comparisons and operon predictions, it is hypothesized that GNA1162 is part of a transport system and assists in transport and reassembly. The crystal structure of GNA1162 sheds light on its possible function and provides potentially valuable information for the design of a vaccine against meningococcal disease.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, People’s Republic of China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Cai
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wu
authors.forename: Chunting
authors.initials: C
authors.lastname: Yang
authors.forename: Honglin
authors.initials: H
authors.lastname: Xu
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Lin
authors.forename: Yuequan
authors.initials: Y
authors.lastname: Shen
mesh_terms: D008074:Lipoproteins / Q000737:chemistry*; D008958:Models, Molecular; D009345:Neisseria meningitidis / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D040681:Structural Homology, Protein
chemical_list: D008074:Lipoproteins
keywords: GNA1162; lipoproteins; outer membrane proteins; transport systems
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