title: Toehold-mediated nonenzymatic DNA strand displacement as a platform for DNA genotyping.
abstract: Toehold-mediated DNA strand displacement provides unique advantages in the construction and manipulation of multidimensional DNA nanostructures as well as nucleic acid sequence analysis. We demonstrate a step change in the use of toehold-mediated DNA strand displacement reactions, where a double-stranded DNA duplex, containing a single-stranded toehold domain, enzymatically generated and then treated as a molecular target for analysis. The approach was successfully implemented for human DNA genotyping, such as gender identification where the amelogenin gene was used as a model target system, and detecting single nucleotide polymorphisms of human mitochondrial DNA. Kinetics of the strand displacement was monitored by the quenched Förster resonance energy transfer effect.
authors.affiliation: Flinders Centre for Nanoscale Science and Technology, Flinders University, GPO Box 2100, Adelaide, S.A, 5001 Australia. dmitriy.khodakov@flinders.edu.au
authors.forename: Dmitriy A
authors.initials: DA
authors.lastname: Khodakov
authors.forename: Anastasia S
authors.initials: AS
authors.lastname: Khodakova
authors.forename: Adrian
authors.initials: A
authors.lastname: Linacre
authors.forename: Amanda V
authors.initials: AV
authors.lastname: Ellis
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics*; D031541:Fluorescence Resonance Energy Transfer; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D004277:DNA, Single-Stranded