title: Low level laser therapy (AlGaInP) applied at 5J/cm2 reduces the proliferation of Staphylococcus aureus MRSA in infected wounds and intact skin of rats.
abstract: BACKGROUND
  Laser therapy is a low cost, non-invasive procedure with good healing results. Doubts exist as to whether laser therapy action on microorganisms can justify research aimed at investigating its possible effects on bacteria-infected wounds.
  
  
  OBJECTIVE
  To assess the effect of low intensity laser on the rate of bacterial contamination in infected wounds in the skin of rats.
  
  
  METHODS
  An experimental study using 56 male Wistar rats. The animals were randomly divided into eight groups of seven each. Those in the "infected" groups were infected by Staphylococcus aureus MRSA in the dorsal region. Red laser diode (AlGaInP) 658nm, 5J/cm2 was used to treat the animals in the "treated" groups in scan for 3 consecutive days. Samples were drawn before inoculating bacteria and following laser treatment. For statistical analysis we used the nonparametric Wilcoxon (paired data) method with a significance level of p <0.05.
  
  
  RESULTS
  The statistical analysis of median values showed that the groups submitted to laser treatment had low bacterial proliferation.
  
  
  CONCLUSIONS
  The laser (AlGaInP), with a dose of 5J/cm2 in both intact skin and in wounds of rats infected with Staphylococcus aureus MRSA, is shown to reduce bacterial proliferation.
authors.affiliation: Faculdade Inspirar Petrolina, Petrolina, PE, Brazil. danielaftgomes@hotmail.com
authors.forename: Daniela Conceição Gomes Gonçalves e
authors.initials: DC
authors.lastname: Silva
authors.forename: Helio
authors.initials: H
authors.lastname: Plapler
authors.forename: Mateus Matiuzzi da
authors.initials: MM
authors.lastname: Costa
authors.forename: Silvio Romero Gonçalves e
authors.initials: SR
authors.lastname: Silva
authors.forename: Maria da Conceição Aquino de
authors.initials: Mda C
authors.lastname: Sá
authors.forename: Benedito Sávio Lima e
authors.initials: BS
authors.lastname: Silva
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development / Q000528:radiation effects*; D011829:Radiation Dosage; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000532:radiotherapy*; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000528:radiation effects*; D014946:Wound Infection / Q000382:microbiology / Q000532:radiotherapy*
references.citation: J Clin Laser Med Surg. 2002 Dec;20(6):325-33
references.pmid: 12513919
references.citation: Rev Col Bras Cir. 2010 Aug;37(4):295-302
references.pmid: 21085848
references.citation: Lasers Surg Med. 2003;32(3):197-202
references.pmid: 12605426
references.citation: Curr Microbiol. 2006 Oct;53(4):282-6
references.pmid: 16941244
references.citation: Photomed Laser Surg. 2010 Jun;28(3):291-325
references.pmid: 19995230
references.citation: Photochem Photobiol. 2009 Jan-Feb;85(1):227-33
references.pmid: 18764901
references.citation: JAMA. 2008 Dec 10;300(22):2647-62
references.pmid: 19066385
references.citation: Am J Otolaryngol. 2011 May-Jun;32(3):198-202
references.pmid: 20434806
references.citation: Rev Bras Ter Intensiva. 2007 Sep;19(3):337-41
references.pmid: 25310069
references.citation: Photomed Laser Surg. 2011 Jun;29(6):405-12
references.pmid: 21219239
references.citation: Photomed Laser Surg. 2008 Feb;26(1):6-9
references.pmid: 18248154
references.citation: Rev Bras Fisioter. 2010 Jan-Feb;14(1):45-51
references.pmid: 20414561
references.citation: J Rehabil Res Dev. 2009;46(4):543-54
references.pmid: 19882488
references.citation: Lasers Med Sci. 2006 Sep;21(3):153-9
references.pmid: 16897111