title: Magnetic resonance imaging at 7T reveals common events in age-related sarcopenia and in the homeostatic response to muscle sterile injury.
abstract: Skeletal muscle remodeling in response to various noxae physiologically includes structural changes and inflammatory events. The possibility to study those phenomena in-vivo has been hampered by the lack of validated imaging tools. In our study, we have relied on multiparametric magnetic resonance imaging for quantitative monitoring of muscle changes in mice experiencing age-related sarcopenia or active regeneration after sterile acute injury of tibialis anterior muscle induced by cardiotoxin (CTX) injection. The extent of myofibrils' necrosis, leukocyte infiltration, and regeneration have been evaluated and compared with parameters from magnetic resonance imaging: T2-mapping (T2 relaxation time; T2-rt), diffusion-tensor imaging (fractional anisotropy, F.A.) and diffusion weighted imaging (apparent diffusion coefficient, ADC). Inflammatory leukocytes within the perimysium and heterogeneous size of fibers characterized aged muscles. They displayed significantly increased T2-rt (P<0.05) and F.A. (P<0.05) compared with young muscles. After acute damage T2-rt increased in otherwise healthy young muscles with a peak at day 3, followed by a progressive decrease to basal values. F.A. dropped 24 hours after injury and afterward increased above the basal level in the regenerated muscle (from day 7 to day 15) returning to the basal value at the end of the follow up period. The ADC displayed opposite kinetics. T2-rt positively correlated with the number of infiltrating leucocytes retrieved by immunomagnetic bead sorting from the tissue (r = 0.92) and with the damage/infiltration score (r = 0.88) while F.A. correlated with the extent of tissue regeneration evaluated at various time points after injury (r = 0.88). Our results indicate that multiparametric MRI is a sensitive and informative tool for monitoring inflammatory and structural muscle changes in living experimental animals; particularly, it allows identifying the increase of T2-rt and F.A. as common events reflecting inflammatory infiltration and muscle regeneration in the transient response of the tissue to acute injury and in the persistent adaptation to aging.
authors.affiliation: Department of Radiology and Preclinical MR and US Facility of Experimental Imaging Center, San Raffaele Scientific Institute, Milano, Italy. esposito.antonio@hsr.it
authors.forename: Antonio
authors.initials: A
authors.lastname: Esposito
authors.forename: Lara
authors.initials: L
authors.lastname: Campana
authors.forename: Anna
authors.initials: A
authors.lastname: Palmisano
authors.forename: Francesco
authors.initials: F
authors.lastname: De Cobelli
authors.forename: Tamara
authors.initials: T
authors.lastname: Canu
authors.forename: Francesco
authors.initials: F
authors.lastname: Santarella
authors.forename: Caterina
authors.initials: C
authors.lastname: Colantoni
authors.forename: Antonella
authors.initials: A
authors.lastname: Monno
authors.forename: Michela
authors.initials: M
authors.lastname: Vezzoli
authors.forename: Giulio
authors.initials: G
authors.lastname: Pezzetti
authors.forename: Angelo A
authors.initials: AA
authors.lastname: Manfredi
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rovere-Querini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Del Maschio
mesh_terms: D000375:Aging; D000818:Animals; D054715:Cardiotoxins; D002465:Cell Movement; D056324:Diffusion Tensor Imaging / Q000379:methods*; D005260:Female; D006706:Homeostasis / Q000502:physiology; D007962:Leukocytes / Q000166:cytology; D051379:Mice; D018482:Muscle, Skeletal / Q000293:injuries / Q000473:pathology*; D009210:Myofibrils / Q000473:pathology; D012038:Regeneration / Q000502:physiology*; D055948:Sarcopenia / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology
chemical_list: D054715:Cardiotoxins
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