title: β-Ionone arrests cell cycle of gastric carcinoma cancer cells by a MAPK pathway.
abstract: β-Ionone is an end ring analog of β-carotenoid which has been shown to possess potent anti-proliferative activity both in vitro and in vivo. To investigate the possible inhibitory effects of β-ionone, we studied cell growth characteristics, DNA synthesis, cell cycle progression, as well as mitogen-activated protein kinases (MAPKs) pathways in the human gastric adenocarcinoma cancer cell line (SGC-7901). Our results show that cell growth and DNA synthesis were inhibited, and the cell cycle was arrested at the G0/G1 phase in a dose-dependent manner in cells treated with β-ionone (25, 50, 100 and 200 μmol/L) for 24 h. We found that the β-ionone significantly decreased the extracellular signal-regulated kinase protein expression and significantly increased the levels of p38 and Jun-amino-terminal kinase protein expression (P < 0.01). β-Ionone also inhibited cell cycle-related proteins of Cdk4, Cyclin B1, D1 and increased p27 protein expression in SGC-7901 cells. These results suggested that the cell cycle arrest observed may be regulated through a MAPK pathway by transcriptional down-regulation of cell cycle proteins. These results demonstrate potent ability of β-ionone to arrest cell cycle of SGC-7901 cells and decrease proliferation.
authors.affiliation: Harbin Medical University, 157 BaoJian Road, NanGang District, Harbin, 150081, People's Republic of China, donghongwei0824@yahoo.com.cn.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Dong
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Sun
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Juan C
authors.initials: JC
authors.lastname: Ibla
authors.forename: Sulpicio G
authors.initials: SG
authors.lastname: Soriano
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Han
authors.forename: Lian-Xin
authors.initials: LX
authors.lastname: Liu
authors.forename: Ming-Song
authors.initials: MS
authors.lastname: Li
authors.forename: Jia-Ren
authors.initials: JR
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D045792:Norisoprenoids / Q000008:administration & dosage / Q000494:pharmacology*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D045792:Norisoprenoids; D004247:DNA; C008157:beta-ionone; D048049:Extracellular Signal-Regulated MAP Kinases