title: Creatine supplementation does not decrease oxidative stress and inflammation in skeletal muscle after eccentric exercise.
abstract: Thirty-six male rats were used; divided into 6 groups (n = 6): saline; creatine (Cr); eccentric exercise (EE) plus saline 24 h (saline + 24 h); eccentric exercise plus Cr 24 h (Cr + 24 h); eccentric exercise plus saline 48 h (saline + 48 h); and eccentric exercise plus Cr 48 h (Cr + 48 h). Cr supplementation was administered as a solution of 300 mg · kg body weight(-1) · day(-1) in 1 mL water, for two weeks, before the eccentric exercise. The animals were submitted to one downhill run session at 1.0 km · h(-1) until exhaustion. Twenty-four and forty-eight hours after the exercise, the animals were killed, and the quadriceps were removed. Creatine kinase levels, superoxide production, thiobarbituric acid reactive substances (TBARS) level, carbonyl content, total thiol content, superoxide dismutase, catalase, glutathione peroxidase, interleukin-1b (IL-1β), nuclear factor kappa B (NF-kb), and tumour necrosis factor (TNF) were analysed. Cr supplementation neither decreases Cr kinase, superoxide production, lipoperoxidation, carbonylation, total thiol, IL-1β, NF-kb, or TNF nor alters the enzyme activity of superoxide dismutase, catalase, and glutathione peroxides in relation to the saline group, respectively (P < 0.05). There are positive correlations between Cr kinase and TBARS and TNF-α 48 hours after eccentric exercise. The present study suggests that Cr supplementation does not decrease oxidative stress and inflammation after eccentric contraction.
authors.affiliation: Exercise Biochemistry and Physiology Laboratory, Postgraduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, 88806‑000, Criciúma, SC, Brazil. luciano_acordi@yahoo.com.br
authors.forename: Luciano A
authors.initials: LA
authors.lastname: Silva
authors.forename: Camila B
authors.initials: CB
authors.lastname: Tromm
authors.forename: Guilherme
authors.initials: G
authors.lastname: Da Rosa
authors.forename: Karoliny
authors.initials: K
authors.lastname: Bom
authors.forename: Thais F
authors.initials: TF
authors.lastname: Luciano
authors.forename: Talita
authors.initials: T
authors.lastname: Tuon
authors.forename: Cláudio T
authors.initials: CT
authors.lastname: De Souza
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Pinho
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003401:Creatine / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D019587:Dietary Supplements; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010805:Physical Conditioning, Animal / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology*; D052097:Quadriceps Muscle / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D012420:Running / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; D003402:Creatine Kinase; D003401:Creatine