title: RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.
abstract: Our previous study using suppression subtractive hybridization (SSH), cDNA microarray and semi-quantitative RT-PCR showed that RPS12 was overexpressed in gastric cancer and it was closely related to metastasis. However, the role of RPS12 in gastric cancer is not clear, which led us to conduct the current study to further investigate the effects of RPS12 on the proliferation and migration of gastric cancer cells, and also to explore the underlying molecular mechanisms. RNA interference was used to inhibit the expression of RPS12. The expression of RPS12 and S100A4 in gastric cancer cells was determined using semi-quantitative RT-PCR and western blot analysis. Cell proliferation and migration were detected by MTT and transwell assay, respectively. In addition, the promoter activity of S100A4 was measured by a Dual-Luciferase Reporter Assay System. We found that RNAi‑mediated RPS12 downregulation led to reduced proliferation and migration of BGC823 and SGC7901 gastric cancer cells. Further results showed that RPS12 inhibition led to reduced S100A4 expression and decreased promoter activity of S100A4 in BGC823 cells. We demonstrated that ectopic expression of S100A4 reversed the reduced proliferation and migration ability after RPS12 inhibition in BGC823 cells. Our findings provide the first demonstration that RPS12 plays important roles in regulating the proliferation and migration of gastric cancer cells. S100A4 can mediate the effects of RPS12 as a downstream effector.
authors.affiliation: Department of Medical Genetics, China Medical University, Shenyang 110001, PR China.
authors.forename: Danqi
authors.initials: D
authors.lastname: Chen
authors.forename: Ruixiu
authors.initials: R
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Hao
authors.initials: H
authors.lastname: Fu
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liu
authors.forename: Kailai
authors.initials: K
authors.lastname: Sun
authors.forename: Xiuju
authors.initials: X
authors.lastname: Sun
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D009362:Neoplasm Metastasis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D024101:Mitochondrial Proteins; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C112310:mitochondrial ribosomal protein S12; C077591:S100A4 protein, human