title: Characterization and expression of the gene encoding En-MAPK1, an intestinal cell kinase (ICK)-like kinase activated by the autocrine pheromone-signaling loop in the Polar Ciliate, Euplotes nobilii.
abstract: In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was previously shown to involve the phosphorylation of a nuclear protein kinase structurally similar to the intestinal-cell and male germ cell-associated kinases described in mammals. In E. nobilii, which is phylogenetically closely related to E. raikovi but inhabits Antarctic and Arctic waters, we have now characterized a gene encoding a structurally homologous kinase. The expression of this gene requires +1 translational frameshifting and a process of intron splicing for the production of the active protein, designated En-MAPK1, which contains amino acid substitutions of potential significance for cold-adaptation.
authors.affiliation: Laboratory of Eukaryotic Microbiology and Animal Biology, Department of Environmental and Natural Sciences, University of Camerino, Camerino 62032, Italy. adriana.vallesi@unicam.it.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Candelori
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Luporini
authors.forename: Claudio
authors.initials: C
authors.lastname: Alimenti
authors.forename: Adriana
authors.initials: A
authors.lastname: Vallesi
mesh_terms: D000595:Amino Acid Sequence; D019898:Autocrine Communication / Q000502:physiology*; D003001:Cloning, Molecular; D016812:Euplotes / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010675:Pheromones / Q000378:metabolism*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D010675:Pheromones; D015800:Protozoan Proteins; D019950:Mitogen-Activated Protein Kinase 1
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