title: A time course for the analysis of meiotic progression in Arabidopsis thaliana.
abstract: We have developed a technique that enables the thymidine analogues bromodeoxyuridine (BrdU) or ethynyldeoxyuridine (EdU) to be taken up via the transpiration stream into pollen mother cells and incorporated into the meiotic S-phase. Labelled inflorescences are sampled at intervals, which enables assessment of the progression of labelled meiocytes by detection of the incorporated BrdU or EdU.
authors.affiliation: School of Biosciences, The University of Birmingham, Birmingham, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Armstrong
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D055993:Germ Cells, Plant / Q000254:growth & development; D008540:Meiosis / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods; D016196:S Phase
chemical_list: D001973:Bromodeoxyuridine