title: Species distribution and virulence factors of Candida spp. isolated from the oral cavity of kidney transplant recipients in Brazil.
abstract: Although yeasts belonging to the genus Candida are frequently seen as commensals in the oral cavity, they possess virulence attributes that contribute for pathogenicity. The aims of the present study were to study the prevalence of Candida spp. isolated from the oral cavity of renal transplant recipients and to analyze strains virulence factors. We isolated a total of 70 Candida strains from 111 transplant recipients, and Candida albicans was the most prevalent species (82.86 %). Oral candidiasis was diagnosed in 14.4 % kidney transplant patients, while 11 isolates (15.7 %) corresponded to non-Candida albicans Candida (NCAC) species. C. albicans adhered to a higher extension than NCAC strains. Some isolates of Candida tropicalis were markedly adherent to human buccal epithelial cells and highly biofilm-forming strains. Regarding proteinase activity, Candida orthopsilosis was more proteolytic than Candida metapsilosis. Candida glabrata and Candida dubliniensis showed very low ability to form biofilm on polystyrene microtiter plates. We have demonstrated here diverse peculiarities of different Candida species regarding the ability to express virulence factors. This study will contribute for the understanding of the natural history and pathogenesis of yeasts belonging to the genus Candida in the oral cavity of patients who were submitted to kidney transplant and are under immunosuppressive therapies.
authors.affiliation: Laboratório de Micologia Médica e Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. guilherme.chaves@ufrnet.br
authors.forename: Guilherme Maranhão
authors.initials: GM
authors.lastname: Chaves
authors.forename: Mariana Guimarães
authors.initials: MG
authors.lastname: Diniz
authors.forename: Walicyranison Plinio
authors.initials: WP
authors.lastname: da Silva-Rocha
authors.forename: Luanda Bárbara Ferreira Canário
authors.initials: LB
authors.lastname: de Souza
authors.forename: Libia Augusta Maciel
authors.initials: LA
authors.lastname: Gondim
authors.forename: Maria Angela Fernandes
authors.initials: MA
authors.lastname: Ferreira
authors.forename: Terezinha Inez Estivalet
authors.initials: TI
authors.lastname: Svidzinski
authors.forename: Eveline Pipolo
authors.initials: EP
authors.lastname: Milan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018441:Biofilms / Q000254:growth & development; D001938:Brazil; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002353:Carrier State / Q000382:microbiology*; D002448:Cell Adhesion; D002648:Child; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D010447:Peptide Hydrolases / Q000378:metabolism; D015995:Prevalence; D014180:Transplantation; D037521:Virulence Factors / Q000378:metabolism*; D055815:Young Adult
chemical_list: D037521:Virulence Factors; D010447:Peptide Hydrolases
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