title: Lack of genomic instability in bone marrow cells of SCID mice exposed whole-body to low-dose radiation.
abstract: It is clear that high-dose radiation is harmful. However, despite extensive research, assessment of potential health-risks associated with exposure to low-dose radiation (at doses below or equal to 0.1 Gy) is still challenging. Recently, we reported that 0.05 Gy of 137Cs gamma rays (the existing limit for radiation-exposure in the workplace) was incapable of inducing significant in vivo genomic instability (measured by the presence of late-occurring chromosomal damage at 6 months post-irradiation) in bone marrow (BM) cells of two mouse strains, one with constitutively high and one with intermediate levels of the repair enzyme DNA-dependent protein-kinase catalytic-subunit (DNA-PKcs). In this study, we present evidence for a lack of genomic instability in BM cells of the severely combined-immunodeficiency (SCID/J) mouse (which has an extremely low-level of DNA-PKcs activity) exposed whole-body to low-dose radiation (0.05 Gy). Together with our previous report, the data indicate that low-dose radiation (0.05 Gy) is incapable of inducing genomic instability in vivo (regardless of the levels of DNA-PKcs activity of the exposed mice), yet higher doses of radiation (0.1 and 1 Gy) do induce genomic instability in mice with intermediate and extremely low-levels of DNA-PKcs activity (indicating an important role of DNA-PKcs in DNA repair).
authors.affiliation: Pathology Department, Stony Brook University, Stony Brook, NY 11974, USA. Kanokporn.Rithidech@sbumed.org
authors.forename: Kanokporn Noy
authors.initials: KN
authors.lastname: Rithidech
authors.forename: Chatchanok
authors.initials: C
authors.lastname: Udomtanakunchai
authors.forename: Louise
authors.initials: L
authors.lastname: Honikel
authors.forename: Elbert
authors.initials: E
authors.lastname: Whorton
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000528:radiation effects*; D002586:Cesium; D002869:Chromosome Aberrations / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects*; D042822:Genomic Instability; D008297:Male; D051379:Mice; D016513:Mice, SCID; D011829:Radiation Dosage
chemical_list: D002586:Cesium
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