title: Curcumin loaded NIPAAM/VP/PEG-A nanoparticles: physicochemical and chemopreventive properties.
abstract: This study aims at modifying the synthesis method of preparing N-isopropylacrylamide (NIPAAM)/N-vinyl-2-pyrrolidone (VP)/Polyethylene glycol monoacrylate (PEG-A) polymeric nanoparticles encapsulating curcumin as a model drug. The optimal concentration of nanoparticle reagents was determined using Fourier Transform Infrared Spectroscopy. Curcumin nanoparticles mean hydrodynamic size was found to be 104 nm with zeta potential of 3 ± 13 mV. The release kinetic study of curcumin nanoparticles indicates that a maximum release of curcumin at 24 h positively correlates with increase in temperature; however, change in pH did not produce any substantial drug release. In vitro cell viability assay performed on cancer cells exposed to various concentrations of model compound displayed the IC50 ranging between 100 and 200 μg/mL for human prostate cancer cells (PC3 cells) and 50 and 200 μg/mL for epidermoid carcinoma (A431 cell line). The Hoechst staining and phase contrast micrographs for 48 h exposure of curcumin nanoparticles at a concentration of 400 μg/mL resulted in almost 92% of cells death in both cell lines. This study concludes that the physiochemical characteristics of NIPAAM/VP/PEG-A polymer with key features of water solubility, sustained drug release, small particle size make these nanoparticles a prominent drug delivery device.
authors.affiliation: Department of Experimental Surgery, McGill University, Montreal, QC H3H1P3, Canada.
authors.forename: Pezhman
authors.initials: P
authors.lastname: Salehi
authors.forename: Georges
authors.initials: G
authors.lastname: Makhoul
authors.forename: Ranjan
authors.initials: R
authors.lastname: Roy
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Malhotra
authors.forename: Zhoobin A
authors.initials: ZA
authors.lastname: Mood
authors.forename: Sam J
authors.initials: SJ
authors.lastname: Daniel
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000179:Acrylates / Q000737:chemistry; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage* / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry*; D006801:Humans; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011467:Prostate / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000188:drug therapy; D011760:Pyrrolidinones / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D000179:Acrylates; D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D011760:Pyrrolidinones; D011092:Polyethylene Glycols; C067295:N-isopropylacrylamide; D003474:Curcumin; C028537:2-pyrrolidone