title: Rapid immune colloidal gold strip for cetacean meat restraining illegal trade and consumption: implications for conservation and public health.
abstract: The consumption of cetacean meat is geographically common and often of undetermined sustainability. Besides, it can expose humans to contaminants and zoonotic pathogens. The illegality of possessing cetacean meat was likely under-reported in some countries due to lack of attention paid by the officials although DNA analysis of market products helped to show such practices. We developed two monoclonal antibodies against synthetic peptides of myoglobin (Mb) for constructing a rapid immune colloidal gold strip. Only cetacean Mb is capable of binding to both antibodies and presents positive signal while the Mb from other animals can bind only 1 of the antibodies and presents negative result. The strip for cetacean meat would be an applicable and cost-effective test for field inspectors and even the general public. It contributes to increase the reporting capacity and coverage of illegal cetacean meat possession, which has implications for global cetacean conservation and public health.
authors.affiliation: Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan, ROC.
authors.forename: Chieh
authors.initials: C
authors.lastname: Lo
authors.forename: Li-Te
authors.initials: LT
authors.lastname: Chin
authors.forename: Chi-Shih
authors.initials: CS
authors.lastname: Chu
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Kun-Wei
authors.initials: KW
authors.lastname: Chan
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002591:Cetacea / Q000276:immunology*; D005260:Female; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D008460:Meat / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000032:analysis* / Q000276:immunology; D010455:Peptides / Q000032:analysis / Q000276:immunology; D011634:Public Health; D011934:Reagent Strips / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D009211:Myoglobin; D010455:Peptides; D011934:Reagent Strips
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: J Exp Biol. 1999 Feb;202(Pt 3):227-36
references.pmid: 9882635
references.citation: Immunochemistry. 1975 May;12(5):423-38
references.pmid: 53189
references.citation: Mol Ecol. 2007 Jul;16(13):2607-9
references.pmid: 17594431
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2000 Jun;126(2):181-91
references.pmid: 10936758
references.citation: J Food Sci. 2009 Oct;74(8):C602-7
references.pmid: 19799656
references.citation: Chem Biol Drug Des. 2009 Jul;74(1):33-42
references.pmid: 19519742
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: J Exp Biol. 2004 Sep;207(Pt 20):3441-6
references.pmid: 15339940
references.citation: J Wildl Dis. 1993 Jan;29(1):109-13
references.pmid: 8383251
references.citation: Vet Rec. 2001 May 26;148(21):657-61
references.pmid: 11400986
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2006 Oct;145(2):249-56
references.pmid: 16962803
references.citation: J Hered. 2003 Mar-Apr;94(2):111-4
references.pmid: 12721222
references.citation: Nature. 1975 Aug 7;256(5517):495-7
references.pmid: 1172191
references.citation: J Exp Biol. 2001 Jan;204(Pt 2):209-15
references.pmid: 11136607
references.citation: Environ Health Perspect. 2005 May;113(5):521-6
references.pmid: 15866757
references.citation: Environ Sci Technol. 2005 Aug 1;39(15):5703-8
references.pmid: 16124305
references.citation: Dis Aquat Organ. 2009 Sep 23;86(2):143-57
references.pmid: 19902843
references.citation: Meat Sci. 2007 Jul;76(3):489-94
references.pmid: 22060991