title: Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia.
abstract: OBJECTIVE
  To investigate the expressions of type I collagen, α2 integrin and β1 integrin in the posterior sclera of guinea pigs with defocus myopia and whether basic fibroblast growth factor (bFGF) injection inhibits the formation and development of myopia by upregulating the expression of type I collagen, α2 integrin and β1 integrin.
  
  
  METHODS
  After 14 days of treatment, the refractive state and axial length were measured and the levels of type I collagen, α2 integrin and β1 integrin were assayed in the posterior sclerae of groups of guinea pigs that wore a monocular -7D polymethylmethacrylate (PMMA) lens or had -7D lens wear followed by the peribulbar injection of Phosphate Buffer Solution (PBS) or bFGF. The untreated fellow eye served as a control. Guinea pigs with no treatment served as normal group.
  
  
  RESULTS
  The results showed that 14 days of monocular defocus increased axial eye length and refraction, while bFGF delivery inhibited them markedly. Further, it was also found that the monocular -7D lens could decrease the levels of type I collagen, α2 integrin and β1 integrin expressions, while, unlike PBS, bFGF increased them significantly in comparison to contralateral control eyes and normal eyes.
  
  
  CONCLUSIONS
  bFGF can prevent the formation and development of defocus myopia by upregulating the expressions of type I collagen, α2 integrin and β1 integrin. Taken together, our results demonstrate that bFGF promotes sclera remodeling to prevent myopia in guinea pigs.
authors.affiliation: Department of Ophthalmology, the First Hospital Affiliated to China Medical University, Shenyang 110001, Liaoning Province, China ; Department of Ophthalmology, the Fourth People's Hospital of Shenyang, Shenyang 110001, Liaoning Province China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Tian
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Cheng
authors.forename: Gui-Bo
authors.initials: GB
authors.lastname: Liu
authors.forename: Su-Fen
authors.initials: SF
authors.lastname: Guo
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Fan
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Zhan
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Xu
keywords: bFGF; defocus myopia; type I collagen; α2 integrin; β1 integrin
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