title: Parasitological and molecular diagnosis in experimental Strongyloides venezuelensis infection.
abstract: Strongyloides venezuelensis is a parasitic nematode of rats which is frequently used as a model to study human and animal strongyloidiasis. The aim of this study was to evaluate the correlation between parasitological and molecular diagnosis in Strongyloides venezuelensis infection. PCR assays were used to detect S. venezuelensis DNA in fecal samples obtained from experimentally infected Rattus norvegicus. The results showed a higher sensitivity of the PCR assay in detecting the infection compared to parasitological methods.
authors.affiliation: Hospital das Clínicas da Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, USP, São Paulo, SP, Brazil. fmp@hcnet.usp.br
authors.forename: Fabiana Martins
authors.initials: FM
authors.lastname: Paula
authors.forename: Renata Barnabé
authors.initials: RB
authors.lastname: Sitta
authors.forename: Fernanda Mello
authors.initials: FM
authors.lastname: Malta
authors.forename: Maiara
authors.initials: M
authors.lastname: Gottardi
authors.forename: Marcelo Andreetta
authors.initials: MA
authors.lastname: Corral
authors.forename: Ronaldo César Borges
authors.initials: RC
authors.lastname: Gryschek
authors.forename: Pedro Paulo
authors.initials: PP
authors.lastname: Chieffi
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000032:analysis*; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000235:genetics / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis*
chemical_list: D018509:DNA, Helminth