title: High-resolution 3D imaging and quantification of gold nanoparticles in a whole cell using scanning transmission ion microscopy.
abstract: Increasing interest in the use of nanoparticles (NPs) to elucidate the function of nanometer-sized assemblies of macromolecules and organelles within cells, and to develop biomedical applications such as drug delivery, labeling, diagnostic sensing, and heat treatment of cancer cells has prompted investigations into novel techniques that can image NPs within whole cells and tissue at high resolution. Using fast ions focused to nanodimensions, we show that gold NPs (AuNPs) inside whole cells can be imaged at high resolution, and the precise location of the particles and the number of particles can be quantified. High-resolution density information of the cell can be generated using scanning transmission ion microscopy, enhanced contrast for AuNPs can be achieved using forward scattering transmission ion microscopy, and depth information can be generated from elastically backscattered ions (Rutherford backscattering spectrometry). These techniques and associated instrumentation are at an early stage of technical development, but we believe there are no physical constraints that will prevent whole-cell three-dimensional imaging at <10 nm resolution.
authors.affiliation: Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Ce-Belle
authors.initials: CB
authors.lastname: Chen
authors.forename: Chammika N B
authors.initials: CN
authors.lastname: Udalagama
authors.forename: Minqin
authors.initials: M
authors.lastname: Ren
authors.forename: Kah Ee
authors.initials: KE
authors.lastname: Fong
authors.forename: Lin Yue Lanry
authors.initials: LY
authors.lastname: Yung
authors.forename: Pastorin
authors.initials: P
authors.lastname: Giorgia
authors.forename: Andrew Anthony
authors.initials: AA
authors.lastname: Bettiol
authors.forename: Frank
authors.initials: F
authors.lastname: Watt
mesh_terms: D006046:Gold / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D053768:Metal Nanoparticles; D008853:Microscopy / Q000379:methods*
chemical_list: D006046:Gold
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