title: Optimized GC-MS method to simultaneously quantify acetylated aldose, ketose, and alditol for plant tissues based on derivatization in a methyl sulfoxide/1-methylimidazole system.
abstract: The isomers of monosaccharide always produce multiple chromatographic peaks as volatile derivatives during gas chromatography, which may result in the overlapping of different sugar peaks. Whereas reduction and oximation of sugar carbonyl groups for GC analysis do eliminate many isomer derivatives, the approaches create new problems. One ketose can yield two peaks by oximation, and different aldoses and ketoses can yield the same alditol upon reduction, leading to the inability to detect some important monosaccharides. This paper reports an optimal method that yields a single peak per sugar by acetylation directly. By using a methyl sulfoxide (Me2SO)/1-methylimidazole (1-MeIm) system, the carbohydrates in acetic anhydride (Ac2O) esterification reactions were solubilized, and the oxidation that normally occurs was inhibited. The results demonstrate that acetylated derivatives of 23 saccharides had unique peaks, which indicates aldose, ketose, and alditol can be determined simultaneously by GC-MS.
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University , Beijing 100083, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Shichang
authors.initials: S
authors.lastname: Liu
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Tan
authors.forename: Nan
authors.initials: N
authors.lastname: Chao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Tian
authors.forename: Liwang
authors.initials: L
authors.lastname: Qi
authors.forename: William A
authors.initials: WA
authors.lastname: Powell
authors.forename: Xiangning
authors.initials: X
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Gai
mesh_terms: D000089:Acetic Anhydrides / Q000737:chemistry; D000107:Acetylation; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D020441:Ginkgo biloba / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D007093:Imidazoles / Q000737:chemistry; D007661:Ketoses / Q000032:analysis*; D057230:Limit of Detection; D009005:Monosaccharides / Q000737:chemistry; D032107:Populus / Q000737:chemistry*; D013402:Sugar Alcohols / Q000032:analysis*
chemical_list: D000089:Acetic Anhydrides; D007093:Imidazoles; D007661:Ketoses; D009005:Monosaccharides; D013402:Sugar Alcohols; C031800:acetic anhydride; D005947:Glucose; C018100:1-methylimidazole; D004121:Dimethyl Sulfoxide