title: Inhibitory effect of mitomycin C on proliferation of primary cultured fibroblasts from human airway granulation tissues.
abstract: BACKGROUND
  Airway granulation tissue and scar formation pose a challenge because of the high incidence of recurrence after treatment. As an emerging treatment modality, topical application of mitomycin C has potential value in delaying the recurrence of airway obstruction. Several animal and clinical studies have already proven its feasibility and efficacy. However, the ideal dosage has still not been determined.
  
  
  OBJECTIVE
  To establish a novel method for culturing primary fibroblasts isolated from human airway granulation tissue, and to investigate the dose-effect of mitomycin C on the fibroblast proliferation in vitro, so as to provide an experimental reference for clinical practitioners.
  
  
  METHODS
  Granulation tissues were collected during the routine bronchoscopy at our department. The primary fibroblasts were obtained by culturing the explanted tissues. The cells were treated with different concentrations of mitomycin C (0.1, 0.2, 0.4, 0.8 and 1.6 mg/ml) for 5 min followed by additional 48-hour culture before an MTT assay was performed to measure cell viability.
  
  
  RESULTS
  MTT assay showed that mitomycin C reduced cell viability at all tested concentrations. The inhibitory ratios were 10.26, 26.77, 32.88, 64.91 and 80.45% for cells treated with mitomycin C at 0.1, 0.2, 0.4, 0.8 and 1.6 mg/ml, respectively.
  
  
  CONCLUSIONS
  Explant culture is a reliable method for culturing primary fibroblasts from human airway granulation tissue, and mitomycin C can inhibit proliferation of the fibroblasts in vitro.
authors.affiliation: Department of Pulmonary Medicine, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Yu Ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Ying Hua
authors.initials: YH
authors.lastname: Pei
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001980:Bronchi / Q000166:cytology; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D006097:Granulation Tissue / Q000166:cytology*; D006801:Humans; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage*
chemical_list: D000903:Antibiotics, Antineoplastic; D016685:Mitomycin