title: Does 4-tert-octylphenol affect estrogen signaling pathways in bank vole Leydig cells and tumor mouse Leydig cells in vitro?
abstract: Primary Leydig cells obtained from bank vole testes and the established tumor Leydig cell line (MA-10) have been used to explore the effects of 4-tert-octylphenol (OP). Leydig cells were treated with two concentrations of OP (10(-4) M, 10(-8) M) alone or concomitantly with anti-estrogen ICI 182,780 (1 μM). In OP-treated bank vole Leydig cells, inhomogeneous staining of estrogen receptor α (ERα) within cell nuclei was found, whereas it was of various intensity among MA-10 Leydig cells. The expression of ERα mRNA and protein decreased in both primary and immortalized Leydig cells independently of OP dose. ICI partially reversed these effects at mRNA level while at protein level abrogation was found only in vole cells. Dissimilar action of OP on cAMP and androgen production was also observed. This study provides further evidence that OP shows estrogenic properties acting on Leydig cells. However, its effect is diverse depending on the cellular origin.
authors.affiliation: Department of Endocrinology, Institute of Zoology, Jagiellonian University, Krakow, Poland. malgorzata.kotula-balak@uj.edu.pl
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kotula-Balak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Chojnacka
authors.forename: Anna
authors.initials: A
authors.lastname: Hejmej
authors.forename: Jerzy
authors.initials: J
authors.lastname: Galas
authors.forename: Monika
authors.initials: M
authors.lastname: Satola
authors.forename: Barbara
authors.initials: B
authors.lastname: Bilinska
mesh_terms: D000818:Animals; D003411:Arvicolinae; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D004967:Estrogens / Q000633:toxicity*; D000077267:Fulvestrant; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010636:Phenols / Q000633:toxicity*; D015398:Signal Transduction; D013739:Testosterone / Q000378:metabolism
chemical_list: D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D004967:Estrogens; D010636:Phenols; D000077267:Fulvestrant; D013739:Testosterone; D004958:Estradiol; D000242:Cyclic AMP; C105260:4-tert-octylphenol