title: New inhibitors of ROS generation and T-cell proliferation from Myrtus communis.
abstract: Phytochemical investigation on Myrtus communis Linn. afforded myrtucommuacetalone (1) with an unprecedented carbon skeleton and a new phloroglucinol-type compound, myrtucommulone M (2), along with four known constituents 3-6. Their structures were established by extensive analyses of NMR and mass spectral data as well as by single-crystal X-ray diffraction studies. These constituents were evaluated for their ability to modulate the immune response, based on their effects on various components of immune system. Compounds 1 and 5 exhibited significant inhibitory effect against nitric oxide (NO(•)) production. Compound 1 also exhibited significant antiproliferative activity (IC50 < 0.5 μg/mL) against T-cell proliferation. Myricetin (3) exerted a significant inhibition (IC50 = 1.6 μg/mL) on zymosan-stimulated whole blood phagocytes ROS production. Compounds 1 and 3 were active against PMA-stimulated ROS generation.
authors.affiliation: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Noureen
authors.initials: N
authors.lastname: Khan
authors.forename: Manzoor
authors.initials: M
authors.lastname: Ahmad
authors.forename: Sammer
authors.initials: S
authors.lastname: Yousuf
authors.forename: Hoong-Kun
authors.initials: HK
authors.lastname: Fun
authors.forename: Samreen
authors.initials: S
authors.lastname: Soomro
authors.forename: M
authors.initials: M
authors.lastname: Asif
authors.forename: M Ahmed
authors.initials: MA
authors.lastname: Mesaik
authors.forename: Farzana
authors.initials: F
authors.lastname: Shaheen
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D018360:Crystallography, X-Ray; D005419:Flavonoids / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008968:Molecular Conformation; D015394:Molecular Structure; D031651:Myrtus / Q000737:chemistry*; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D013601:T-Lymphocytes / Q000187:drug effects*; D015054:Zymosan / Q000097:blood
chemical_list: D005419:Flavonoids; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C040015:myricetin; D015054:Zymosan; D010696:Phloroglucinol