title: Polysaccharide extraction from Spirulina sp. and its antioxidant capacity.
abstract: To optimize polysaccharide extraction from Spirulina sp., the effect of solid-to-liquid ratio, extraction temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that extraction temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, temperature of 90°C, and time of 120 min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45 mg GAE/g sample.
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bang Khun Thain, Bangkok 10150, Thailand. ratana.cha@kmutt.ac.th
authors.forename: Ratana
authors.initials: R
authors.lastname: Chaiklahan
authors.forename: Nattayaporn
authors.initials: N
authors.lastname: Chirasuwan
authors.forename: Panya
authors.initials: P
authors.lastname: Triratana
authors.forename: Veara
authors.initials: V
authors.lastname: Loha
authors.forename: Suvit
authors.initials: S
authors.lastname: Tia
authors.forename: Boosya
authors.initials: B
authors.lastname: Bunnag
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification*; D005609:Free Radicals / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D012210:Rhamnose / Q000737:chemistry; D052616:Solid Phase Extraction; D053545:Spirulina / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010636:Phenols; D010851:Picrates; D011135:Polysaccharides, Bacterial; C004931:1,1-diphenyl-2-picrylhydrazyl; D012210:Rhamnose