title: Micro-nanopatterning as tool to study the role of physicochemical properties on cell-surface interactions.
abstract: The current nano-biotechnologies interfacing synthetic materials and cell biology requires a better understanding of cell-surface interactions on the micro-to-nanometer scale. Cell-substrate interactions are mediated by the presence of proteins adsorbed from biological fluids to the substrate. The effect of nanotopography and surface chemistry on protein adsorption as well as the mediation effect on subsequent cellular communication with substratum is not well documented. This review discusses the role of physicochemical properties of cell-surface interactions and state-of-the-art methods currently available for micro-nanoscale surface fabrication and patterning. We also briefly discuss the current surface patterning techniques that allow the combination of a fine and independent control on nanotopography and chemistry to understand the effect of surface nanoscale substrate morphology on cell-surface interactions which has never been realized in previous reports. In addition, we discuss the influence of various chemical patterning and modulation of the nano-topography of surfaces on cell functionality and phenotype.
authors.affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180-3590; Center for Biotechnology and Interdisciplinary Studies, Room 2145, Rensselaer Polytechnic Institute, 110 8th Street, Troy, New York 12180.
authors.forename: Ajay Vikram
authors.initials: AV
authors.lastname: Singh
authors.forename: Rajendra
authors.initials: R
authors.lastname: Patil
authors.forename: Dipalee K
authors.initials: DK
authors.lastname: Thombre
authors.forename: W N
authors.initials: WN
authors.lastname: Gade
mesh_terms: D000818:Animals; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism*; D055598:Chemical Phenomena; D006801:Humans; D055616:Microtechnology / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*
keywords: AFM; ECM; cell-surface interactions; e-beam lithography; micro-nanopatterning