title: Alginate/quaternized carboxymethyl chitosan/clay nanocomposite microspheres: preparation and drug-controlled release behavior.
abstract: Drug-delivery systems, using natural drug carriers, have become increasingly important because of their nontoxicity and biodegradability. In this study, firstly, quaternized carboxymethyl chitosan (QCMC) was intercalated into the interlayer of organic montmorillonite (OMMT) to obtain the QCMC/OMMT nanocomposites, their structure, morphology, and thermal stability were investigated. Next, crosslinked alginate/QCMC/OMMT (AQCOM) microsphere was obtained by crosslinking with CaCl2, and the drug-controlled release behavior was evaluated with bovine serum albumin (BSA) as model drug. The results suggested that, carboxyl groups in alginate and QCMC crosslinked with Ca(2+), quaternary ammonium groups in QCMC or OMMT electrostatically interacted with carboxyl groups in alginate, and there was stable three-dimensional network in AQCOM microsphere. The swelling ratio of AQCOM microspheres decreased with the increase of OMMT content, the lowest one was only about 45% compared to the microsphere without OMMT of 197%. Besides, the in vitro release results for BSA indicated that the AQCOM microsphere displayed more excellent encapsulation and controlled release capacities than the microsphere without OMMT. The in vitro active cutaneous anaphylaxis test was carried out on Guinea pigs, which revealed that AQCOM microsphere did not cause anaphylaxis. Therefore, QCMC/OMMT nanocomposites from natural materials are considerably suitable to apply as drug-controlled release carriers.
authors.affiliation: State Key Laboratory of Pulp & Paper Engineering, School of Light Industry and Food, South China University of Technology, Guangzhou 510640, Guangdong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Jiwen
authors.initials: J
authors.lastname: Luo
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.forename: Junxiang
authors.initials: J
authors.lastname: Lu
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
authors.forename: Runcang
authors.initials: R
authors.lastname: Sun
mesh_terms: D000464:Alginates / Q000009:adverse effects / Q000737:chemistry*; D000707:Anaphylaxis / Q000139:chemically induced; D000818:Animals; D001546:Bentonite / Q000009:adverse effects / Q000737:chemistry*; D002417:Cattle; D048271:Chitosan / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000009:adverse effects / Q000737:chemistry*; D020723:Glucuronic Acid / Q000009:adverse effects / Q000737:chemistry; D006168:Guinea Pigs; D006603:Hexuronic Acids / Q000009:adverse effects / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D012867:Skin / Q000187:drug effects
chemical_list: D000464:Alginates; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; C514968:carboxymethyl-chitosan; D001546:Bentonite; D012710:Serum Albumin, Bovine; D020723:Glucuronic Acid; D048271:Chitosan