title: Fumigant toxicity of Citrus sinensis essential oil on Musca domestica L. adults in the absence and presence of a P450 inhibitor.
abstract: Essential oils (EOs) are potential tools for controlling Musca domestica L. In a fumigant assay, M. domestica adults treated with Citrus sinensis EO (LC50=3.9mg/dm(3)), with (4R)(+)-limonene (95.1%) being its main component, died within 15min or less. The terpenes absorbed by the flies and their metabolites, analyzed using SPME fiber, were (4R)(+)-limonene (LC50=6.2mg/dm(3)), α-pinene (LC50=11.5mg/dm(3)), β-pinene (LC50=6.4mg/dm(3)), and two new components, carveol (LC50=1122mg/dm(3)) and carvone (LC50=19mg/dm(3)), in a proportion of 50, 6.2, 12.5, 6.3 and 25%, respectively. Carveol and carvone were formed by oxidation of (4R)(+)-limonene mediated by cytochrome P450, as was suggested by a fumigation assay on flies previously treated with piperonyl butoxide, a P450 inhibitor. In this experiment, an increase in the toxicity of the EO and (4R)(+)-limonene was observed, as well as a lower production of carveol and carvone.
authors.affiliation: Laboratorio de Química Fina y Productos Naturales, Universidad Católica de Córdoba, Avenida Armada Argentina 3555 (5016), Córdoba, Argentina.
authors.forename: Yanina Estefanía
authors.initials: YE
authors.lastname: Rossi
authors.forename: Sara María
authors.initials: SM
authors.lastname: Palacios
mesh_terms: D000818:Animals; D032084:Citrus sinensis / Q000737:chemistry*; D065607:Cytochrome P-450 Enzyme Inhibitors; D005260:Female; D006793:Houseflies / Q000187:drug effects*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D015394:Molecular Structure; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010882:Piperonyl Butoxide / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D007306:Insecticides; D009822:Oils, Volatile; D010938:Plant Oils; D013729:Terpenes; D010882:Piperonyl Butoxide