title: In-depth proteome analysis of the rubber particle of Hevea brasiliensis (para rubber tree).
abstract: The rubber particle is a special organelle in which natural rubber is synthesised and stored in the laticifers of Hevea brasiliensis. To better understand the biological functions of rubber particles and to identify the candidate rubber biosynthesis-related proteins, a comprehensive proteome analysis was performed on H. brasiliensis rubber particles using shotgun tandem mass spectrometry profiling approaches-resulting in a thorough report on the rubber particle proteins. A total of 186 rubber particle proteins were identified, with a range in relative molecular mass of 3.9-194.2 kDa and in isoelectric point values of 4.0-11.2. The rubber particle proteins were analysed for gene ontology and could be categorised into eight major groups according to their functions: including rubber biosynthesis, stress- or defence-related responses, protein processing and folding, signal transduction and cellular transport. In addition to well-known rubber biosynthesis-related proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and cis-prenyl transferase (CPT), many proteins were firstly identified to be on the rubber particles, including cyclophilin, phospholipase D, cytochrome P450, small GTP-binding protein, clathrin, eukaryotic translation initiation factor, annexin, ABC transporter, translationally controlled tumour protein, ubiquitin-conjugating enzymes, and several homologues of REF, SRPP and CPT. A procedure of multiple reaction monitoring was established for further protein validation. This comprehensive proteome data of rubber particles would facilitate investigation into molecular mechanisms of biogenesis, self-homeostasis and rubber biosynthesis of the rubber particle, and might serve as valuable biomarkers in molecular breeding studies of H. brasiliensis and other alternative rubber-producing species.
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou, Hainan, PR China. dailongjun@gmail.com
authors.forename: Longjun
authors.initials: L
authors.lastname: Dai
authors.forename: Guijuan
authors.initials: G
authors.lastname: Kang
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Nie
authors.forename: Cuifang
authors.initials: C
authors.lastname: Duan
authors.forename: Rizhong
authors.initials: R
authors.lastname: Zeng
mesh_terms: D015153:Blotting, Western; D005591:Chemical Fractionation; D002853:Chromatography, Liquid; D028482:Hevea / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D015151:Immunoblotting; D010455:Peptides / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012408:Rubber / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013312:Stress, Physiological
chemical_list: D010455:Peptides; D010940:Plant Proteins; D020543:Proteome; D012408:Rubber
references.citation: Plant Cell Physiol. 2003 Apr;44(4):412-4
references.pmid: 12721382
references.citation: Annu Rev Plant Biol. 2010;61:291-315
references.pmid: 20192745
references.citation: PLoS One. 2012;7(6):e39494
references.pmid: 22768084
references.citation: Mol Syst Biol. 2008;4:222
references.pmid: 18854821
references.citation: Eur J Biochem. 2003 Dec;270(23):4671-80
references.pmid: 14622254
references.citation: Nat Biotechnol. 2009 Jul;27(7):633-41
references.pmid: 19561596
references.citation: Plant Mol Biol. 2011 Jan;75(1-2):167-78
references.pmid: 21107886
references.citation: Planta. 2011 Oct;234(4):785-98
references.pmid: 21630042
references.citation: Mol Cell Proteomics. 2006 Apr;5(4):573-88
references.pmid: 16332733
references.citation: J Plant Res. 2002 Jun;115(3):169-77
references.pmid: 12579366
references.citation: Phytochemistry. 2003 Sep;64(1):123-34
references.pmid: 12946411
references.citation: Plant J. 2008 Aug;55(4):639-51
references.pmid: 18445132
references.citation: Nat Methods. 2007 Mar;4(3):207-14
references.pmid: 17327847
references.citation: Proteomics. 2009 Nov;9(21):4871-80
references.pmid: 19743414
references.citation: J Exp Bot. 2007;58(10):2429-40
references.pmid: 17545224
references.citation: J Proteome Res. 2010 Dec 3;9(12):6696-704
references.pmid: 20968308
references.citation: Plant Physiol. 1991 Sep;97(1):317-21
references.pmid: 16668388
references.citation: Plant Physiol. 2012 Mar;158(3):1406-17
references.pmid: 22238421
references.citation: Planta. 1999 Nov;210(1):85-96
references.pmid: 10592036
references.citation: Proteome Sci. 2010 Jun 18;8:35
references.pmid: 20565811
references.citation: Plant Cell Physiol. 2006 Jul;47(7):839-52
references.pmid: 16672254
references.citation: Plant Cell. 2008 Dec;20(12):3430-47
references.pmid: 19060111
references.citation: Bioinform Biol Insights. 2011 Feb 07;5:41-58
references.pmid: 21423406
references.citation: Curr Opin Mol Ther. 2002 Jun;4(3):242-50
references.pmid: 12139310
references.citation: Science. 2009 Sep 4;325(5945):1217-20
references.pmid: 19729648
references.citation: Mol Cells. 2012 Jun;33(6):617-26
references.pmid: 22610367
references.citation: J Neurochem. 2006 Aug;98(4):1126-40
references.pmid: 16895580
references.citation: J Exp Bot. 2003 Mar;54(384):985-92
references.pmid: 12598569
references.citation: J Biol Chem. 1999 Jun 11;274(24):17132-8
references.pmid: 10358068
references.citation: Arch Biochem Biophys. 2011 Mar 1;507(1):194-203
references.pmid: 20920462
references.citation: Trends Cell Biol. 2001 Dec;11(12):487-91
references.pmid: 11719054
references.citation: Cell. 1998 Feb 6;92(3):367-80
references.pmid: 9476896
references.citation: Genes Dev. 2002 Mar 15;16(6):707-19
references.pmid: 11914276
references.citation: Proteomics. 2012 Mar;12(6):901-5
references.pmid: 22539439
references.citation: Phytochemistry. 2001 Aug;57(7):1123-34
references.pmid: 11430985
references.citation: Genome Res. 2010 Feb;20(2):265-72
references.pmid: 20019144
references.citation: Plant Physiol Biochem. 2011 Jul;49(7):729-37
references.pmid: 21530287
references.citation: Planta. 2008 May;227(6):1213-9
references.pmid: 18270732
references.citation: Prog Nucleic Acid Res Mol Biol. 1998;60:47-78
references.pmid: 9594571
references.citation: BMC Biochem. 2010 Feb 19;11:11
references.pmid: 20170509
references.citation: Phytochemistry. 2009 Mar;70(5):570-8
references.pmid: 19356777
references.citation: Annu Rev Biochem. 2011;80:273-99
references.pmid: 21548781
references.citation: Bioinformatics. 2010 Apr 1;26(7):966-8
references.pmid: 20147306
references.citation: Traffic. 2009 Jul;10(7):819-28
references.pmid: 19416470
references.citation: Plant Physiol. 2000 Aug;123(4):1227-34
references.pmid: 10938342
references.citation: PLoS One. 2012;7(7):e41874
references.pmid: 22911861
references.citation: Plant J. 1998 Aug;15(3):441-7
references.pmid: 9750355
references.citation: Nat Cell Biol. 2010 Jul;12(7):645-54
references.pmid: 20562865
references.citation: J Biol Chem. 1989 Nov 5;264(31):18608-17
references.pmid: 2681199
references.citation: Trends Biotechnol. 2007 Nov;25(11):522-9
references.pmid: 17936926
references.citation: Plant Cell. 2004 Dec;16(12):3285-303
references.pmid: 15539469
references.citation: Plant Mol Biol. 2003 Nov;53(4):479-92
references.pmid: 15010614
references.citation: Plant Physiol. 2000 Jul;123(3):1133-42
references.pmid: 10889262
references.citation: J Biol Chem. 2010 Apr 2;285(14):10627-37
references.pmid: 20154091
references.citation: J Biol Chem. 1995 Apr 14;270(15):8487-94
references.pmid: 7721745
references.citation: Plant Physiol Biochem. 2012 Nov;60:207-13
references.pmid: 22995218
references.citation: J Exp Bot. 2012 Mar;63(5):1863-71
references.pmid: 22162870
references.citation: Tree Physiol. 2010 Oct;30(10):1349-59
references.pmid: 20660491