title: In vivo antitumor activity of biosynthesized silver nanoparticles using Ficus religiosa as a nanofactory in DAL induced mice model.
abstract: Ficus religiosa leaf extract was chosen as a reducing agent to fabricate silver nanoparticles (AgNPs) by a simple, cost-effective and eco-friendly process with the aim of treating Dalton's ascites lymphoma (DAL) in mice model. The formation of synthesized nanoparticles were characterized by UV-visible analysis (UV-vis), Fourier transform infra-red (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD) and zeta potential analyses. A peak at 431nm indicated the surface plasmon resonance of AgNPs. FTIR studies indicated polyphenols and proteins as possible encapsulates. TEM analysis showed particles size in the range of 5-35nm. Healthy Swiss Albino mice (30-35g) were intraperitoneally induced with DAL cells and treated with F. religiosa derived AgNPs at a dose of 50μg/ml. Blood and liver tissues were collected subsequent to dissection and subjected to hematological, biochemical and anticancer assays. Hematological and biochemical analyses revealed revival after treating with F. religiosa derived AgNPs. Antioxidant activity results further proved supportive evidence. The apoptosis inducing effect of AgNPs was observed through acridine orange staining (AO and EB) and DNA fragmentation assay. Anti- angiogenic activity was confirmed by observing vessel development. All these observations indicate that the AgNPs were effective in treatment of DAL.
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli-24, Tamil Nadu, India.
authors.forename: Jacob Joe
authors.initials: JJ
authors.lastname: Antony
authors.forename: Mohamed Ali Ayisha
authors.initials: MA
authors.lastname: Sithika
authors.forename: Thomas Amal
authors.initials: TA
authors.lastname: Joseph
authors.forename: Udhayaraj
authors.initials: U
authors.lastname: Suriyakalaa
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Sankarganesh
authors.forename: Durairaj
authors.initials: D
authors.lastname: Siva
authors.forename: Seenivasan
authors.initials: S
authors.lastname: Kalaiselvi
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Achiraman
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001201:Ascites / Q000188:drug therapy* / Q000473:pathology; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D030681:Ficus / Q000737:chemistry*; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D008223:Lymphoma / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009389:Neovascularization, Pathologic; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D012834:Silver