title: Association of monocyte chemoattractant protein-1 (MCP-1) 2518A/G polymorphism with proliferative diabetic retinopathy in Korean type 2 diabetes.
abstract: OBJECTIVE
  Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that can increase adhesion molecule expression on monocytes and produce superoxide anions. Hyperglycemia induces MCP-1 production in vascular endothelial cells and retinal pigmented epithelial cells, and has been implicated as a causal factor in the facilitation of vascular complications in diabetes. In the present study, we evaluated the association of a single nucleotide polymorphism (SNP) in the MCP-1 gene with proliferative diabetic retinopathy (PDR) in a Korean population with type 2 diabetes.
  
  
  METHODS
  We conducted a case-control study, which enrolled 590 subjects with type 2 diabetes, and SNP genotyping of c.2518A/G in the MCP-1 gene was performed using polymerase chain reaction followed by digestion with PvuII restriction enzyme.
  
  
  RESULTS
  The prevalence of c.2518A/G polymorphism in diabetic patients was 13.2% (A/A), 47.1% (A/G) and 39.7% (G/G). In patients with diabetic retinopathy, the prevalence of PDR was significantly higher (p=0.009) in diabetic subjects with the c.2518A/A genotype (35.9%; n=78) compared to those with either the A/G or G/G genotype (22.3%, n=512). The prevalence of any other micro and macro-complications, including nephropathy and cerebrovascular events, were not different according to the c.2518A/G genotype.
  
  
  CONCLUSIONS
  Our new genetic findings suggest that the c.2518A/A genotype in MCP-1 could be used as a susceptibility gene to predispose Koreans exhibiting type 2 diabetes for the development of PDR.
authors.affiliation: Department of Internal Medicine, Chungbuk National University College of Medicine, 776 1sunhwan-ro, Heungdeok-gu, Cheongju 361-711, Korea.
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Jeon
authors.forename: Hyung Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Byong Hee
authors.initials: BH
authors.lastname: Park
authors.forename: Yong Hee
authors.initials: YH
authors.lastname: Lee
authors.forename: Taekeun
authors.initials: T
authors.lastname: Oh
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D003930:Diabetic Retinopathy / Q000209:etiology / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D056910:Republic of Korea
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2
keywords: MCP-1 polymorphism; proliferative diabetic retinopathy; type 2 DM
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