title: Combined treatments of enterocin AS-48 with biocides to improve the inactivation of methicillin-sensitive and methicillin-resistant Staphylococcus aureus planktonic and sessile cells.
abstract: Control of staphylococci during cleaning and disinfection is important to the food industry. Broad-spectrum bacteriocins with proved anti-staphylococcal activity, such as enterocin AS-48, could open new possibilities for disinfection in combination with biocides. In the present study, enterocin AS-48 was tested singly or in combination with biocides against a cocktail of six Staphylococcus aureus strains (including three methicillin-resistant strains) in planktonic state as well as in biofilms formed on polystyrene microtiter plates. Cells were challenged with enterocin, biocides or enterocin/biocide combinations. Inactivation of planktonic cells increased significantly (p<0.05) when enterocin AS-48 (25mg/l) was tested in combination with benzalkonium chloride (BC), cetrimide (CT) and hexadecylpyridinium chloride (HDP), and non-significantly in combination with didecyldimethylammonium bromide (AB), triclosan (TC), hexachlorophene (CF), polyhexamethylen guanidinium chloride (PHMG), chlorhexidine (CH) or P3-oxonia (OX). In the sessile state (24h biofilms), staphylococci required higher biocide concentrations in most cases, except for OX. Inactivation of sessile staphylococci increased remarkably when biocides were applied in combination with enterocin AS-48, especially when the bacteriocin was added at 50mg/l. During storage, the concentrations of sessile as well as planktonic cells in the treated samples decreased remarkably for BC, TC and PHMG, but OX failed to inhibit proliferation of the treated biofilms as well as growth of planktonic cells. The observed inhibitory effects during storage were potentiated when the biocides were combined with 50 mg/l enterocin AS-48. Results from this study suggest that selected combinations of enterocin AS-48 and biocides offer potential use against planktonic and sessile, methicillin-sensitive and methicillin-resistant S. aureus.
authors.affiliation: Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071 Jaén, Spain.
authors.forename: Natacha
authors.initials: N
authors.lastname: Caballero Gómez
authors.forename: Hikmate
authors.initials: H
authors.lastname: Abriouel
authors.forename: M José
authors.initials: MJ
authors.lastname: Grande
authors.forename: Rubén
authors.initials: R
authors.lastname: Pérez Pulido
authors.forename: Antonio
authors.initials: A
authors.lastname: Gálvez
mesh_terms: D001430:Bacteriocins / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000592:standards; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D050296:Microbial Viability / Q000187:drug effects; D010933:Plankton / Q000187:drug effects; D013997:Time Factors
chemical_list: D001430:Bacteriocins; D004202:Disinfectants; C466236:enterocin AS-48, Enterococcus faecalis