title: Evaluation of diabetic polyneuropathy in Type 2 diabetes mellitus by nerve conduction study and association of severity of neuropathy with serum sFasL level.
abstract: BACKGROUND
  Diabetes mellitus (DM), a growing health problem globally, has reached epidemic proportions in India. Recently, Fas-mediated apoptosis has been proposed as a causative factor responsible for neuronal degeneration in diabetic polyneuropathy (DPN), but there are very few studies to show association of serum soluble Fas ligand (sFasL) level with severity of neuropathy.
  
  
  OBJECTIVE
  The aim of this study was to investigate whether serum sFasL, a transmembrane glycoprotein involved in apoptosis, has any association with severity of peripheral neuropathy in Type 2 DM.
  
  
  METHODS
  The study was conducted in Department of Physiology in collaboration with Department of Endocrinology, IPGME&R. sFasL levels in serum were assessed using ELISA method in healthy individuals (n = 16), newly diagnosed diabetic controls (n = 16) without any complications, and in DPN cases (n = 33) with predominant neuropathy only. All subjects underwent both electrodiagnostic procedures and vibration perception threshold (VPT) for quantitative assessment of the severity of neuropathy. Using nerve conduction studies, amplitudes, velocities, and latencies of both sensory and motor nerves were recorded.
  
  
  RESULTS
  In DPN patients, concentration of sFasL levels (87.53 ± 3.49) was significantly decreased (P < 0.0001) not only when compared with normal controls (225.30 ± 2.97) but also when compared with diabetic patients without any complication (161 ± 3.63). Moreover, the concentration of sFasL is significantly (P < 0.0001) associated with the severity of neuropathy both by VPT and nerve conduction velocity (NCV).
  
  
  CONCLUSIONS
  Fas-mediated apoptosis is involved in Type 2 DM and might be associated with the severity of polyneuropathy.
authors.affiliation: Department of Physiology, IPGME&R, Kolkata, India.
authors.forename: Avijit
authors.initials: A
authors.lastname: Mondal
authors.forename: Sangita
authors.initials: S
authors.lastname: Sen
authors.forename: Debarati
authors.initials: D
authors.lastname: Chanda
authors.forename: Sunanda
authors.initials: S
authors.lastname: Kundu
authors.forename: Mitali
authors.initials: M
authors.lastname: Chatterjee
authors.forename: Satinath
authors.initials: S
authors.lastname: Mukherjee
keywords: Diabetic polyneuropathy; nerve conduction velocity; soluble Fas ligand
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