title: Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter molecules.
abstract: Molecularly imprinted polymers were prepared using a protein-conjugated disulfide cleavable monomer. After removing the protein by disulfide reduction, a thiol-reactive fluorophore was introduced into the thiol residue located only inside the imprinted cavity, resulting in specific transduction of the binding events into fluorescence spectral change.
authors.affiliation: Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan. takeuchi@gold.kobe-u.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suga
authors.forename: Hirobumi
authors.initials: H
authors.lastname: Sunayama
authors.forename: Tooru
authors.initials: T
authors.lastname: Ooya
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D004220:Disulfides / Q000737:chemistry; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D004220:Disulfides; D011108:Polymers; D011506:Proteins