title: Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation.
abstract: The bovine immunodeficiency virus (BIV) transactivator (BTat) recruits the bovine cyclin T1 (B-cyclin T1) to the LTR to facilitate the transcription of BIV. Here, we demonstrate that bovine hexamethylene bisacetamide (HMBA)-induced protein 1 (BHEXIM1) inhibits BTat-mediated BIV LTR transcription. The results of in vivo and in vitro assays show direct binding of BHEXIM1 to the B-cyclin T1. These results suggest that the repression arises from BHEXIM1-BTat competition for B-cyclin T1, which allows BHEXIM1 to displace BTat from B-cyclin T1. Furthermore, we found that the C-terminal region and the centrally located region of BHEXIM1 are required for BHEXIM1 to associate with B-cyclin T1. Knockdown of BHEXIM1 enhances BIV replication. Taken together, our study provides the first clear evidence that BHEXIM1 is involved in BIV replication through regulating BTat-mediated transactivation.
authors.affiliation: Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University, Tianjin, 300071, China. juantan@nankai.edu.cn.
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Guo
authors.forename: Yong-gang
authors.initials: YG
authors.lastname: Ma
authors.forename: Yuan-ming
authors.initials: YM
authors.lastname: Gai
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Liang
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Yang
authors.initials: Y
authors.lastname: Su
authors.forename: Qi-cheng
authors.initials: QC
authors.lastname: Zhang
authors.forename: Qi-min
authors.initials: QM
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
mesh_terms: D000818:Animals; D002417:Cattle; D056906:Cyclin T / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli; D016087:Immunodeficiency Virus, Bovine / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000662:veterinary; D042925:Positive Transcriptional Elongation Factor B / Q000235:genetics* / Q000378:metabolism; D012191:Retroviridae Proteins / Q000378:metabolism*; D020079:Terminal Repeat Sequences; D015533:Transcriptional Activation
chemical_list: D056906:Cyclin T; D012191:Retroviridae Proteins; D042925:Positive Transcriptional Elongation Factor B
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