title: Insulin sensitizing effect of 3 Indian medicinal plants: an in vitro study.
abstract: OBJECTIVE
  Measurement of glucose uptake into peripheral tissue is an important mechanism to assess Insulin sensitivity. The present in vitro study was conducted to evaluate the Insulin sensitizing activity of Phyllanthus emblica (Pe), Tinospora cordifolia (Tc) and Curcuma longa (Cl) by assessing glucose uptake activity in a 3T3L1 adipocyte model.
  
  
  METHODS
  The 3T3 L1 fibroblast cells were differentiated to adipocytes, using a cocktail of insulin, isobutyl-1-methylxanthine and dexamethazone. These adipocytes were initially treated with different concentrations of the selected plants following which 2-deoxy glucose uptake was estimated using a radioactive assay. The effects of plants on glucose uptake both in the presence and absence of insulin was evaluated and compared with pioglitazone, a known insulin sensitizer.
  
  
  RESULTS
  Pe and Tc per se significantly stimulated glucose uptake in 3T3-L1 adipocytes in a dose dependent manner with maximal effect at higher concentrations (200 μg/ml). The effect of both Pe and Tc at 200 μg/ml was comparable to insulin and greater than pioglitazone. Cl per se stimulated glucose uptake with maximal effect at 50 μg/ml. However, this effect was lesser as compared to insulin with higher concentrations inhibiting glucose uptake. When combined with insulin, an antagonist effect was observed between Pe, Tc and insulin indicating a possible plant-drug interaction while Cl in combination with insulin showed an increase in the glucose uptake as compared to Cl alone.
  
  
  CONCLUSIONS
  The results suggest that one of the mechanisms for the anti-diabetic effect of Pe, Cl and Tc may be through an insulin sensitizing effect (stimulation of glucose uptake into adipocytes). Further studies using other target sites viz. skeletal muscle and hepatocytes models and in an insulin resistant state would help substantiate this conclusion.
authors.affiliation: Department of Clinical Pharmacology, TN Medical College and BYL Nair Charitable Hospital, Mumbai Central, Mumbai - 400 008, India.
authors.forename: Samidha A
authors.initials: SA
authors.lastname: Kalekar
authors.forename: Renuka P
authors.initials: RP
authors.lastname: Munshi
authors.forename: Supriya S
authors.initials: SS
authors.lastname: Bhalerao
authors.forename: Urmila M
authors.initials: UM
authors.lastname: Thatte
mesh_terms: D041721:3T3-L1 Cells / Q000187:drug effects* / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D030024:Curcuma / Q000737:chemistry; D003847:Deoxyglucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D051379:Mice; D029842:Phyllanthus emblica / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D031610:Tinospora / Q000737:chemistry
chemical_list: D007328:Insulin; D010936:Plant Extracts; D003847:Deoxyglucose
keywords: 3T3 L1 adipocytes; Curcuma longa; Glucose uptake; Phyllanthus emblica; Tinospora cordifolia
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