title: Expression of estrogen receptor α 36 (ESR36) in the hamster ovary throughout the estrous cycle: effects of gonadotropins.
abstract: Estradiol-17β (E) plays an important role in ovarian follicular development. Evidence indicates that some of the effect of E is mediated by the transmembrane estrogen receptor. In this study, we examined the spatio-temporal expression of recently discovered ERα36 (ESR36), a splice variant of Esr1 and a receptor for non-genomic E signaling, in the hamster ovary during the estrous cycle and the role of gonadotropins and ovarian steroid hormones in ESR36 expression. ESR36 expression was high on estrus (D1:0900 h) and declined precipitously by proestrus (D4:0900 h) and remained low up to D4:1600 h. Immunofluorescence findings corroborated immunoblot findings and revealed that ESR36 was expressed only in the cell membrane of both follicular and non-follicular cells, except the oocytes. Ovarian ESR36 was capable of binding to the E-affinity matrix, and have different molecular weight than that of the ESR1 or GPER. Hypophysectomy (Hx) resulted in a marked decline in ESR36 protein levels. FSH and LH, alone or combined, markedly upregulated ESR36 protein in Hx hamsters to the levels observed in D1 hamsters, but neither E nor P had any effect. Inhibition of the gonadotropin surge by phenobarbital treatment on D4:1100 h attenuated ESR36 expression in D1:0900 h ovaries, but the decline was restored by either FSH or LH replacement on D4 afternoon. This is the first report to show that ESR36, which is distinct from ESR1 or GPER is expressed in the plasma membrane of ovarian follicular and non-follicular cells, binds to E and its expression is regulated directly by the gonadotropins. In light of our previous findings, the results suggest that ovarian cells contain at least two distinct membrane estrogen receptors, such as GPER and ESR36, and strongly suggest for a non-genomic action of E regulating ovarian follicular functions.
authors.affiliation: Departments of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors.forename: Prabuddha
authors.initials: P
authors.lastname: Chakraborty
authors.forename: Shyamal K
authors.initials: SK
authors.lastname: Roy
mesh_terms: D000818:Animals; D006224:Cricetinae; D047628:Estrogen Receptor alpha / Q000096:biosynthesis*; D030762:Estrous Cycle / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D006062:Gonadotropins / Q000494:pharmacology*; D008647:Mesocricetus; D010053:Ovary / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; D006062:Gonadotropins
references.citation: Pharmacol Rev. 2000 Dec;52(4):513-56
references.pmid: 11121509
references.citation: Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9063-8
references.pmid: 16754886
references.citation: Biol Reprod. 1987 Aug;37(1):39-46
references.pmid: 3115324
references.citation: Biol Reprod. 2004 Jul;71(1):146-55
references.pmid: 14985249
references.citation: J Cell Biochem. 1999 Oct 1;75(1):138-46
references.pmid: 10462712
references.citation: Trends Endocrinol Metab. 2001 May-Jun;12(4):152-6
references.pmid: 11295570
references.citation: Endocrinology. 2002 Jun;143(6):2385-98
references.pmid: 12021204
references.citation: Arterioscler Thromb Vasc Biol. 2002 Dec 1;22(12):1952-61
references.pmid: 12482819
references.citation: J Reprod Fertil. 1986 Sep;78(1):201-9
references.pmid: 3093677
references.citation: J Cell Mol Med. 2010 Jun;14(6B):1485-93
references.pmid: 19840189
references.citation: Endocrinology. 1999 Jun;140(6):2733-44
references.pmid: 10342864
references.citation: Endocrinology. 1972 Apr;90(4):992-1007
references.pmid: 5011067
references.citation: Endocrinology. 1995 Oct;136(10):4610-20
references.pmid: 7664681
references.citation: Mol Endocrinol. 1995 Nov;9(11):1441-54
references.pmid: 8584021
references.citation: Endocrinology. 1974 May;94(5):1295-9
references.pmid: 4856727
references.citation: Am J Anat. 1974 Jan;139(1):37-48
references.pmid: 4810015
references.citation: Endocr Rev. 2001 Jun;22(3):389-424
references.pmid: 11399749
references.citation: Mol Cell Endocrinol. 1999 May 25;151(1-2):57-64
references.pmid: 10411320
references.citation: Endocrinology. 1974 Nov;95(5):1282-6
references.pmid: 4473328
references.citation: J Clin Endocrinol Metab. 1995 Apr;80(4):1438-43
references.pmid: 7714121
references.citation: Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11162-6
references.pmid: 8248223
references.citation: J Appl Physiol (1985). 2001 Oct;91(4):1860-7
references.pmid: 11568173
references.citation: PLoS One. 2010 Feb 02;5(2):e9013
references.pmid: 20126312
references.citation: Endocrinology. 2007 Oct;148(10):4853-64
references.pmid: 17640985
references.citation: Endocr Rev. 1999 Jun;20(3):358-417
references.pmid: 10368776
references.citation: Oncogene. 2001 Sep 6;20(39):5420-30
references.pmid: 11571639
references.citation: Mol Endocrinol. 1999 Feb;13(2):307-19
references.pmid: 9973260
references.citation: Biol Reprod. 2000 Jun;62(6):1858-65
references.pmid: 10819793
references.citation: Biochem Biophys Res Commun. 2005 Nov 4;336(4):1023-7
references.pmid: 16165085
references.citation: Microsc Res Tech. 1994 Feb 1;27(2):83-96
references.pmid: 8123910
references.citation: Endocrinology. 2008 Sep;149(9):4452-61
references.pmid: 18499747
references.citation: Biol Reprod. 2004 Jun;70(6):1580-8
references.pmid: 14749302
references.citation: Am J Obstet Gynecol. 2011 Sep;205(3):227.e1-6
references.pmid: 21684519
references.citation: Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8517-21
references.pmid: 8078914
references.citation: Histochem Cell Biol. 2009 Mar;131(3):347-54
references.pmid: 18956209
references.citation: Nat Med. 1999 Oct;5(10):1130-1
references.pmid: 10502813
references.citation: Physiol Rev. 1980 Jan;60(1):51-89
references.pmid: 6243782
references.citation: Science. 2005 Mar 11;307(5715):1625-30
references.pmid: 15705806
references.citation: Development. 2000 Oct;127(19):4277-91
references.pmid: 10976058