title: Oxidative status in ICU patients with septic shock.
abstract: The aim of this pilot study was to investigate variability of oxidative stress during sepsis evolution. ICU patients with the diagnosis of septic shock were included. Thiobarbituric-acid reactive substances, total antioxidant capacity, protein carbonyls in plasma, reduced, oxidized glutathione and catalase activity in erythrocyte lysate were assessed in the 1st, 3rd, 5th and 8th day after sepsis appearance. A total of 17 patients were divided in two groups: survivors (n=7) and non-survivors (n=10). APACHE II was 11.5 ± 5.4 and 19.9 ± 4.97 in survivors and non-survivors respectively (p=0.005), while mean age and SOFA score at sepsis diagnosis, were similar between the two groups. GSH levels, catalase activity and protein carbonyls presented significant different course in time between survivors and non-survivors (p<0.05). Catalase activity was significantly higher in survivors (238.8 ± 51.5) than non-survivors (166.4 ± 40.2; p=0.005), while protein carbonyls levels were significantly lower in survivors (0.32 ± 0.09) than non-survivors (0.48 ± 0.16; p=0.036) on the 1st day. Yet, non-survivors exhibited a declining course in GSH levels during time, while GSH levels were maintained in survivors. Conclusively, a longstanding antioxidant deficiency in non-surviving patients was noted. This phenomenon was clearly prominent in patients' erythrocytes.
authors.affiliation: Intensive Care Department, University Hospital of Thessaly Biopolis, Larissa 41110, Greece. Electronic address: mkarapetsa@gmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Karapetsa
authors.forename: Marina
authors.initials: M
authors.lastname: Pitsika
authors.forename: Nikos
authors.initials: N
authors.lastname: Goutzourelas
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stagos
authors.forename: Aphrodite
authors.initials: A
authors.lastname: Tousia Becker
authors.forename: Epaminondas
authors.initials: E
authors.lastname: Zakynthinos
mesh_terms: D018806:APACHE; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D019803:Glutathione Disulfide / Q000097:blood; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D050050:Protein Carbonylation; D012772:Shock, Septic / Q000097:blood* / Q000401:mortality; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D019803:Glutathione Disulfide
keywords: APACHE II; ARDS; Acute Physiology And Chronic Health Evaluation II score; C-reactive protein; CAT; CRP; Catalase; FSR; GSH; GSSG; Glutathione; ICU; MOF; Oxidative stress; RBCs; ROS; SIRS; SOFA; Sepsis; Sequential Organ Failure Assessment score; TAC; TBARS; acute respiratory distress syndrome; catalase; fractional synthetic rate; intensive care unit; multiple organ failure; oxidized glutathione; reactive oxygen species; red blood cells; reduced glutathione; systemic inflammatory response syndrome; thiobarbituric acid-reactive substances; total antioxidant capacity