title: Growth temperature and genome size in bacteria are negatively correlated, suggesting genomic streamlining during thermal adaptation.
abstract: Prokaryotic genomes are small and compact. Either this feature is caused by neutral evolution or by natural selection favoring small genomes-genome streamlining. Three separate prior lines of evidence argue against streamlining for most prokaryotes. We find that the same three lines of evidence argue for streamlining in the genomes of thermophile bacteria. Specifically, with increasing habitat temperature and decreasing genome size, the proportion of genomic DNA in intergenic regions decreases. Furthermore, with increasing habitat temperature, generation time decreases. Genome-wide selective constraints do not decrease as in the reduced genomes of host-associated species. Reduced habitat variability is not a likely explanation for the smaller genomes of thermophiles. Genome size may be an indirect target of selection due to its association with cell volume. We use metabolic modeling to demonstrate that known changes in cell structure and physiology at high temperature can provide a selective advantage to reduce cell volume at high temperatures.
authors.affiliation: Department of Immunology, Weizmann Institute of Science, Rehovot, Israel. nsabath@gmail.com
authors.forename: Niv
authors.initials: N
authors.lastname: Sabath
authors.forename: Evandro
authors.initials: E
authors.lastname: Ferrada
authors.forename: Aditya
authors.initials: A
authors.lastname: Barve
authors.forename: Andreas
authors.initials: A
authors.lastname: Wagner
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D001105:Archaea / Q000235:genetics; D001419:Bacteria / Q000235:genetics*; D019143:Evolution, Molecular; D059646:Genome Size; D020745:Genome, Archaeal; D016680:Genome, Bacterial; D006358:Hot Temperature; D011387:Prokaryotic Cells; D012641:Selection, Genetic
keywords: genome evolution; streamlining; thermophilic bacteria
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