title: Simultaneous determination of banned acid orange dyes and basic orange dyes in foodstuffs by liquid chromatography-tandem electrospray ionization mass spectrometry via negative/positive ion switching mode.
abstract: Simultaneous detection of two classes of dyes possessing different chemical properties is difficult. In this study, through negative/positive ion switching mode, simultaneous determination of four typical acid orange dyes and three typical basic orange dyes was achieved by a single high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, and the analytical efficiency of multiresidues identification was greatly improved. To enhance detection sensitivity, the sample pretreatment conditions and HPLC-MS/MS determining conditions were carefully optimized. Under optimal conditions, good linearity was obtained over the range of 5-500 μg L(-1) with a correlation coefficient (R(2)) >0.9998. Limits of detection (LODs) and limits of quantification (LOQs) of the seven dyes were 0.5-3.0 and 2.0-6.0 μg kg(-1), respectively. The recoveries of the seven dyes in soybean products and marinated eggs were 74-126% with relative standard deviations (RSDs) of 2.22-25.4%, suggesting the developed method is promising for the accurate quantification of the seven dyes at trace levels in foods.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology , Tianjin 300457, China.
authors.forename: Guozhen
authors.initials: G
authors.lastname: Fang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Dong
authors.forename: Cuicui
authors.initials: C
authors.lastname: Liu
authors.forename: Shaoyuan
authors.initials: S
authors.lastname: He
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
mesh_terms: D001391:Azo Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D004531:Eggs / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis* / Q000331:legislation & jurisprudence; D013058:Mass Spectrometry / Q000379:methods*; D013025:Glycine max / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents