title: Decreased expression of the DBC2 gene and its clinicopathological significance in breast cancer: correlation with aberrant DNA methylation.
abstract: Loss of DBC2 (deleted in breast cancer 2) gene expression is frequent in breast cancer tissues. This can be explained by homozygous deletions or other mutations in a minority of cases but alternative mechanisms need to be investigated. Here, DBC2 expression was significantly suppressed compared with normal breast tissues in breast cancer tissues when analyzed by RT-PCR. Furthermore, DNA methylation on DBC2 was more prevalent in breast tumors than in normal tissues. DBC2 mRNA levels correlated with the degree of DBC2 methylation in breast cancer tissues and in a breast cancer cell line (T47D). Clinico-pathological correlation analysis showed that DBC2 promoter methylation was associated with tumor-node-metastasis stages II and III/IV, lymph node metastasis, p53 mutation, and HER2-positive status. Thus loss of DBC2 expression is caused by abnormal methylation of DBC2 and might have a role in breast cancer development.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao, 266021, Shandong, China. qdhllabc@126.com
authors.forename: Linlin
authors.initials: L
authors.lastname: Han
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Jinlian
authors.initials: J
authors.lastname: Song
authors.forename: Dongliang
authors.initials: D
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Yinlin
authors.initials: Y
authors.lastname: Ge
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D019175:DNA Methylation; D005260:Female; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: C497336:RHOBTB2 protein, human; D025521:Tumor Suppressor Proteins; D019204:GTP-Binding Proteins