title: In vitro and computational studies of transdermal perfusion of nanoformulations containing a large molecular weight protein.
abstract: Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66kDa) of 160nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a range of medical and cosmetic applications.
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Madalena
authors.initials: M
authors.lastname: Martins
authors.forename: Nuno G
authors.initials: NG
authors.lastname: Azoia
authors.forename: Artur
authors.initials: A
authors.lastname: Ribeiro
authors.forename: Ulyana
authors.initials: U
authors.lastname: Shimanovich
authors.forename: Carla
authors.initials: C
authors.lastname: Silva
authors.forename: Artur
authors.initials: A
authors.lastname: Cavaco-Paulo
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002417:Cattle; D004655:Emulsions; D005456:Fluorescent Dyes; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D008970:Molecular Weight; D009226:Myristates / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry*; D013552:Swine
chemical_list: D004655:Emulsions; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D009226:Myristates; D013501:Surface-Active Agents; C008205:isopropyl myristate; D012710:Serum Albumin, Bovine