title: Inverse correlation between Naa10p and MMP-9 expression  and the combined prognostic value in breast cancer patients.
abstract: To analyze the expression profiles of N-a-acetyltransferase 10 protein (Naa10p/ARD1) and matrix metalloproteinase 9 (MMP-9) in human breast cancer and evaluate their possible prognostic values in breast cancer patients. Quantitative RT-PCR was used to evaluate mRNA expression of Naa10p and MMP-9 in 55 cases of fresh breast cancer tissues, and immunohistochemistry was performed for detecting Naa10p and MMP-9 proteins on breast cancer with tissue microarray containing 80 specimens. Furthermore, Naa10p and MMP-9 were measured in 253 breast cancer tissues from patients with up to 15-year follow-up. Survival curves were generated using the Kaplan-Meier method. Multivariate analysis was performed by using the Cox proportional hazard regression model to assess the prognostic values of Naa10p and MMP-9. Both Naa10p and MMP-9 expression in breast cancer tissues were significantly higher than those in the matched non-cancerous tissues (p = 0.000 for both). There was an inverse correlation between Naa10p and MMP-9 expression at mRNA and protein levels (p = 0.000 for both). Patients with MMP-9- positive expression had a poorer overall survival (OS) and disease-free survival (DFS) than those with MMP-9-negative expression (p = 0.001 for both). However, patients with Naa10p-positive expression had better OS and DFS (p = 0.000 for both). In addition, Naa10p-positive/MMP-9- negative patients had the best OS and DFS (p = 0.000 for both). In multivariate survival analysis, TNM stage, Naa10p expression, MMP-9 expression, and combined expression status of Naa10p/MMP-9 were independent prognostic factors related to OS (p = 0.000, 0.007, 0.012, and 0.000, respectively) and DFS (p = 0.000, 0.002, 0.014, and 0.000, respectively).The expression level of Naa10p was inversely correlated with that of MMP-9 in human breast cancer samples. Combined analysis of Naa10p and MMP-9 had a significantly increased value for determining the prognosis of breast cancer patients.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, 52 Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.forename: Caiyun
authors.initials: C
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Beihai
authors.initials: B
authors.lastname: Jiang
authors.forename: Ye
authors.initials: Y
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Meng
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Like
authors.initials: L
authors.lastname: Qu
authors.forename: Chengchao
authors.initials: C
authors.lastname: Shou
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000201:enzymology* / Q000401:mortality; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D063209:N-Terminal Acetyltransferase A / Q000096:biosynthesis / Q000235:genetics*; D063213:N-Terminal Acetyltransferase E / Q000096:biosynthesis / Q000235:genetics*; D011379:Prognosis; D015996:Survival Rate / Q000639:trends
chemical_list: D014408:Biomarkers, Tumor; D063209:N-Terminal Acetyltransferase A; C504824:NAA10 protein, human; D063213:N-Terminal Acetyltransferase E; D020780:Matrix Metalloproteinase 9
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