title: HIV-1 Vpu does not degrade interferon regulatory factor 3.
abstract: It has been reported that HIV-1 Vpu mediates the degradation of interferon regulatory factor 3 (IRF-3) to avoid innate immune sensing. Here, we show that Vpu does not deplete IRF-3 from transfected cell lines or HIV-1-infected primary cells. Furthermore, the Vpu-dependent suppression of beta interferon expression described in previous studies could be ascribed to inhibition of NF-κB activation. Thus, Vpu suppresses innate immune activation through inhibition of NF-κB rather than degradation of IRF-3.
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Hotter
authors.forename: Frank
authors.initials: F
authors.lastname: Kirchhoff
authors.forename: Daniel
authors.initials: D
authors.lastname: Sauter
mesh_terms: D015497:HIV-1 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006367:HeLa Cells; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D054298:Human Immunodeficiency Virus Proteins; D050838:Interferon Regulatory Factor-3; D016328:NF-kappa B; D054334:Viral Regulatory and Accessory Proteins; C494232:IRF3 protein, human; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins
references.citation: Cell Host Microbe. 2008 Apr 17;3(4):245-52
references.pmid: 18342597
references.citation: J Virol. 2009 Oct;83(20):10322-35
references.pmid: 19656876
references.citation: J Virol. 1992 Dec;66(12):7193-200
references.pmid: 1433512
references.citation: J Virol. 2012 Aug;86(16):8499-506
references.pmid: 22647704
references.citation: J Virol. 1995 Mar;69(3):1510-20
references.pmid: 7853484
references.citation: Blood. 2010 Sep 16;116(11):1876-84
references.pmid: 20530791
references.citation: J Virol. 2009 Oct;83(20):10395-405
references.pmid: 19706707
references.citation: J Gen Virol. 2007 Feb;88(Pt 2):613-620
references.pmid: 17251580
references.citation: J Virol. 2009 Aug;83(16):7931-47
references.pmid: 19515779
references.citation: Nature. 2008 Jan 24;451(7177):425-30
references.pmid: 18200009
references.citation: J Virol. 2012 Apr;86(8):4496-504
references.pmid: 22301152
references.citation: Cell Host Microbe. 2009 Mar 19;5(3):285-97
references.pmid: 19286137
references.citation: Cell Host Microbe. 2010 Nov 18;8(5):397-409
references.pmid: 21075351
references.citation: PLoS Pathog. 2009 May;5(5):e1000443
references.pmid: 19461879
references.citation: Cell. 2006 Jun 16;125(6):1055-67
references.pmid: 16777597
references.citation: Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7381-5
references.pmid: 8346259
references.citation: Cell Host Microbe. 2012 Nov 15;12(5):633-44
references.pmid: 23159053
references.citation: PLoS Pathog. 2009 Feb;5(2):e1000300
references.pmid: 19214216
references.citation: J Virol. 1989 Sep;63(9):3784-91
references.pmid: 2788224
references.citation: Virology. 2008 Mar 30;373(1):85-97
references.pmid: 18082865
references.citation: PLoS Pathog. 2009 Sep;5(9):e1000574
references.pmid: 19730691
references.citation: J Virol. 2012 Aug;86(16):8367-74
references.pmid: 22593165
references.citation: J Virol. 2006 Aug;80(16):8047-59
references.pmid: 16873261
references.citation: Cell Host Microbe. 2009 Nov 19;6(5):409-21
references.pmid: 19917496
references.citation: EMBO J. 1998 May 15;17(10):2777-89
references.pmid: 9582271
references.citation: J Virol. 2005 Aug;79(16):10108-25
references.pmid: 16051804
references.citation: Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):4114-9
references.pmid: 15741273
references.citation: PLoS Pathog. 2012 Dec;8(12):e1003093
references.pmid: 23308067