title: Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis.
abstract: Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.
authors.affiliation: R&D Center, Diatech Korea Co. Ltd., Seoul 138-200, Republic of Korea.
authors.forename: Youn Jee
authors.initials: YJ
authors.lastname: Yeo
authors.forename: Kyoungmin
authors.initials: K
authors.lastname: Roh
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Bang
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Lee
authors.forename: Hyung Soon
authors.initials: HS
authors.lastname: Park
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D020137:Base Pair Mismatch; D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000737:chemistry / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004279:DNA, Viral; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; D004247:DNA