title: Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment.
abstract: A keratinase isolated from Paecilomyces lilacinus (LPS #876) was tested against proteins present in the skin but the high enzyme activity was detected on collagen. Keratinase was physically immobilized onto PVA-pectin cryogels and enzyme release was 20.8±2.1%, 63.8±0.2%, 41.5±3.5% and 26.0±3.5% in cryogels containing pectins with esterification degrees (DE) 33.0%, 55.0%, 62.7% and 71.7% respectively at 37°C after 3h incubation. In presence of 0.75 M NaCl, the percentage of enzyme release changed to: 57.5±1.5, 65.8±3.8, 57.3±0.2 and 34.0±4.0 for the four pectins respectively. In-vitro studies of enrofloxacin release from PVA-pectin cryogels at pH close to the human skin (pH=5.5) showed 15.0% free antibiotic following first order kinetic at 37°C after 5h incubation. However, in the presence of keratinase only 6.9% of enrofloxacin was released under the same experimental conditions.
authors.affiliation: Institute of Applied Biotechnology (CINDEFI, UNLP-CONICET-CCT La Plata), School of Sciences, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: Yanina N
authors.initials: YN
authors.lastname: Martínez
authors.forename: Ivana
authors.initials: I
authors.lastname: Cavello
authors.forename: Roque
authors.initials: R
authors.lastname: Hours
authors.forename: Sebastián
authors.initials: S
authors.lastname: Cavalitto
authors.forename: Guillermo R
authors.initials: GR
authors.lastname: Castro
mesh_terms: D000287:Administration, Topical; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D059985:Cryogels / Q000627:therapeutic use; D000077422:Enrofloxacin; D004800:Enzymes, Immobilized / Q000494:pharmacology*; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D007239:Infections / Q000188:drug therapy*; D010368:Pectins; D010447:Peptide Hydrolases / Q000494:pharmacology*; D057968:Transdermal Patch; D014947:Wounds and Injuries / Q000382:microbiology*
chemical_list: D000891:Anti-Infective Agents, Local; D059985:Cryogels; D004800:Enzymes, Immobilized; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D010368:Pectins; D010447:Peptide Hydrolases; C035855:keratinase
keywords: Controlled release; Enrofloxacin; Keratinase; PVA cryogels; Wound healing