title: A miniaturized immunoassay platform to measure neutrophil gelatinase-associated lipocalin (NGAL) for diagnosis of acute kidney injury.
abstract: In this study, we describe the development and evaluation of a slide-based immunoassay platform for the detection of neutrophil gelatinase associated-lipocalin (NGAL) in plasma and urine samples. The capture NGAL antibody was immobilized onto a microscope slide before an analysis of NGAL based on a sandwich immunoassay was further carried out. This assay system exhibited linearity between 50 to 1000 ng/ml of NGAL. The coefficients of variability (CVs) indicated good reproducibility and repeatability of the system. The levels of plasma NGAL measured by the slide-based system were highly correlated with those of ELISA, while this system over-predicted urine NGAL.
authors.affiliation: Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology, Thonburi, Bangkok, Thailand.
authors.forename: Thanaporn
authors.initials: T
authors.lastname: Bovornvirakit
authors.forename: Yingyos
authors.initials: Y
authors.lastname: Avihingsanon
authors.forename: Khajohn
authors.initials: K
authors.lastname: Tiranathanagul
authors.forename: Kwanchanok
authors.initials: K
authors.lastname: Viravaidya-Pasuwat
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood / Q000175:diagnosis* / Q000473:pathology / Q000652:urine; D000209:Acute-Phase Proteins / Q000652:urine*; D000328:Adult; D000906:Antibodies / Q000737:chemistry; D002138:Calibration; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D000071068:Lipocalin-2; D054834:Lipocalins / Q000097:blood* / Q000652:urine*; D008297:Male; D008904:Miniaturization; D011518:Proto-Oncogene Proteins / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000209:Acute-Phase Proteins; D000906:Antibodies; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins