title: The disordered C-terminal domain of human DNA glycosylase NEIL1 contributes to its stability via intramolecular interactions.
abstract: NEIL1 [Nei (endonuclease VIII)-like protein 1], one of the five mammalian DNA glycosylases that excise oxidized DNA base lesions in the human genome to initiate base excision repair, contains an intrinsically disordered C-terminal domain (CTD; ~100 residues), not conserved in its Escherichia coli prototype Nei. Although dispensable for NEIL1's lesion excision and AP lyase activities, this segment is required for efficient in vivo enzymatic activity and may provide an interaction interface for many of NEIL1's interactions with other base excision repair proteins. Here, we show that the CTD interacts with the folded domain in native NEIL1 containing 389 residues. The CTD is poised for local folding in an ordered structure that is induced in the purified fragment by osmolytes. Furthermore, deletion of the disordered tail lacking both Tyr and Trp residues causes a red shift in NEIL1's intrinsic Trp-specific fluorescence, indicating a more solvent-exposed environment for the Trp residues in the truncated protein, which also exhibits reduced stability compared to the native enzyme. These observations are consistent with stabilization of the native NEIL1 structure via intramolecular, mostly electrostatic, interactions that were disrupted by mutating a positively charged (Lys-rich) cluster of residues (amino acids 355-360) near the C-terminus. Small-angle X-ray scattering (SAXS) analysis confirms the flexibility and dynamic nature of NEIL1's CTD, a feature that may be critical to providing specificity for NEIL1's multiple, functional interactions.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Muralidhar L
authors.initials: ML
authors.lastname: Hegde
authors.forename: Susan E
authors.initials: SE
authors.lastname: Tsutakawa
authors.forename: Pavana M
authors.initials: PM
authors.lastname: Hegde
authors.forename: Luis Marcelo F
authors.initials: LM
authors.lastname: Holthauzen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Numan
authors.initials: N
authors.lastname: Oezguen
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Hilser
authors.forename: John A
authors.initials: JA
authors.lastname: Tainer
authors.forename: Sankar
authors.initials: S
authors.lastname: Mitra
mesh_terms: D045647:DNA Glycosylases / Q000737:chemistry* / Q000378:metabolism*; D004252:DNA Mutational Analysis; D004795:Enzyme Stability; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D025941:Protein Interaction Mapping; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D053838:Scattering, Small Angle
chemical_list: D045647:DNA Glycosylases; C473634:NEIL1 protein, human
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