title: Induction of β-Defensin Expression by Porphyromonas gingivalis-Infected Human Gingival Graft Transplanted in nu/nu Mouse Subdermis.
abstract: It is important to understand the onset of periodontal disease in terms of bacterial infection and host factors. Host-bacteria interactions can be elicited in human cultured cells and animal models, but these models provide only limited biological information about human host reactions against bacterial attacks. Development of an in vivo model using human gingival tissue is needed. We established an in vivo model using nu/nu mice and evaluated host defense following bacterial infection in human gingiva. Human gingival samples were collected from periodontitis patients and transplanted in nu/nu mouse subdermis. After 2 weeks, human characteristics were confirmed by positive immunohistochemical reactions for human-specific markers. We used this model to investigate human β-defensin-2 (hBD-2), an antimicrobial peptide that contributes to initial defense against bacterial invasion. Using real-time polymerase chain reaction, in situ hybridization, and immunohistochemistry, we investigated whether hBD-2 expression was induced in human gingiva as a response to Porphyromonas gingivalis as a periodontal pathogen. Two hours after infection with bacteria, we detected increased expression of hBD-2 mRNA, which was localized in the epithelium of human gingiva. Using our in vivo model, we concluded that increased hBD-2 may play an important role in early defense from bacterial infection in human gingival epithelium.
authors.affiliation: Department of Environmental Pathology and Research Institute of Salivary Gland Health Medicine, Kanagawa Dental College Postgraduate School, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: To
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kamata
authors.forename: Juri
authors.initials: J
authors.lastname: Saruta
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shimizu
authors.forename: Takenori
authors.initials: T
authors.lastname: Sato
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kondo
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Nobushiro
authors.initials: N
authors.lastname: Hamada
authors.forename: Keiichi
authors.initials: K
authors.lastname: Tsukinoki
keywords: human gingiva; human β-defensin-2 (hBD-2); in vivo model; nude mice; periodontal disease
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