title: Improved sample preparation and rapid UHPLC analysis of SO2 binding carbonyls in wine by derivatisation to 2,4-dinitrophenylhydrazine.
abstract: Sulphur dioxide (SO2) is essential for the preservation of wines. The presence of SO2 binding compounds in musts and wines may limit sulphite efficacy leading to higher total SO2 additions, which may exceed SO2 limits permitted by law and pose health risks for sensitive individuals. An improved method for the quantification of significant wine SO2 binding compounds is presented that applies a novel sample treatment approach and rapid UHPLC separation. Glucose, galacturonic acid, alpha-ketoglutarate, pyruvate, acetoin and acetaldehyde were derivatised with 2,4-dinitrophenylhydrazine and separated using a solid core C18 phase by ultra high performance liquid chromatography. Addition of EDTA to samples prevented de novo acetaldehyde formation from ethanol oxidation. Optimised derivatisation duration enhanced reproducibility and allowed for glucose and galacturonic acid quantification. High glucose residues were found to interfere with the recovery of other SO2 binders, but practical SO2 concentrations and red wine pigments did not affect derivatisation efficiency. The calibration range, method accuracy, precision and limits of detection were found to be satisfactory for routine analysis of SO2 binders in wines. The current method represents a significant improvement in the comprehensive analysis of SO2 binding wine carbonyls. It allows for the quantification of major SO2 binders at practical analyte concentrations, and uses a simple sample treatment method that prevents treatment artifacts. Equipment utilisation could be reduced by rapid LC separation while maintaining analytical performance parameters. The improved method will be a valuable addition for the analysis of total SO2 binder pools in oenological samples.
authors.affiliation: Cornell University, NYSAES, Department of Food Science, Geneva, NY 14456, USA.
authors.forename: J N
authors.initials: JN
authors.lastname: Jackowetz
authors.forename: R
authors.initials: R
authors.lastname: Mira de Orduña
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D010084:Oxidation-Reduction; D010659:Phenylhydrazines / Q000737:chemistry*; D013458:Sulfur Dioxide / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D010659:Phenylhydrazines; D013458:Sulfur Dioxide; C004787:2,4-dinitrophenylhydrazine