title: Influence of oily vehicles on fetal testis and lipid profile of rats exposed to di-butyl phthalate.
abstract: It has been hypothesized that oils containing high levels of omega-3 polyunsaturated fatty acids, such as canola and fish oil, could counteract some of the adverse effects induced by phthalates. In the present study, the influence of different oily vehicles on di-butyl phthalate (DBP)-induced testicular toxicity and lipid profile was investigated. Pregnant Wistar rats were treated by oral gavage from gestation days 13 to 20 with DBP (500 mg/kg/day) diluted in three different vehicles: corn, canola or fish oil. Male fetuses were analyzed on gestation day 20. DBP exposure lowered intratesticular testosterone levels and anogenital distance, regardless of the vehicle used. The percentage of seminiferous cords containing multinucleated gonocytes and cord diameter was increased in DBP-exposed groups, compared with vehicle controls, with no difference between the three DBP-exposed groups. Clustering of Leydig cells was seen in all DBP groups. Lipid profile indicated that administration of canola and fish oil can increase the content of omega-3 fatty acids in rat testis. However, content of omega-3 was diminished in DBP-treated groups. Overall, our results indicate that different oily vehicles did not alter fetal rat testicular toxicity induced by a high DBP dose.
authors.affiliation: 1Department of Pharmacology, Federal University of Paraná (UFPR), Curitiba, PR, Brazil.
authors.forename: A C S
authors.initials: AC
authors.lastname: Lourenço
authors.forename: C
authors.initials: C
authors.lastname: Gomes
authors.forename: A C
authors.initials: AC
authors.lastname: Boareto
authors.forename: R P
authors.initials: RP
authors.lastname: Mueller
authors.forename: F
authors.initials: F
authors.lastname: Nihi
authors.forename: L F
authors.initials: LF
authors.lastname: Andrade
authors.forename: E S
authors.initials: ES
authors.lastname: Trindade
authors.forename: I
authors.initials: I
authors.lastname: Coelho
authors.forename: K
authors.initials: K
authors.lastname: Naliwaiko
authors.forename: R N
authors.initials: RN
authors.lastname: Morais
authors.forename: A J
authors.initials: AJ
authors.lastname: Martino-Andrade
mesh_terms: D000818:Animals; D003314:Corn Oil / Q000737:chemistry / Q000378:metabolism; D003993:Dibutyl Phthalate / Q000008:administration & dosage / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000633:toxicity*; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D047109:Fetal Development / Q000187:drug effects; D005395:Fish Oils / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D014677:Pharmaceutical Vehicles / Q000737:chemistry / Q000378:metabolism*; D010968:Plasticizers / Q000008:administration & dosage / Q000633:toxicity; D011247:Pregnancy; D000074262:Rapeseed Oil; D051381:Rats; D019849:Sex Determination Processes / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D005229:Fatty Acids, Monounsaturated; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D014677:Pharmaceutical Vehicles; D010968:Plasticizers; D000074262:Rapeseed Oil; D003993:Dibutyl Phthalate; D013739:Testosterone; D003314:Corn Oil
keywords: Di-butyl phthalate; fatty acids; rats; testis; vehicles