title: Screening of genomic libraries.
abstract: Microsatellites, or simple sequence repeats (SSRs), have proven to be an important molecular marker in plant genetics and breeding research. The main strategies to obtain these markers can be through genomic DNA and from expressed sequence tags (ESTs) from mRNA/cDNA libraries. Genetic studies using microsatellite markers have increased rapidly because they can be highly polymorphic, codominant markers and they show heterozygous conserved sequences. Here, we describe a methodology to obtain microsatellite using the enrichment library of DNA genomic sequences. This method is highly efficient to development microsatellite markers especially in plants that do not have available ESTs or genome databases. This methodology has been used to enrich SSR marker libraries in Citrus spp., an important tool to genotype germplasm, to select zygotic hybrids, and to saturate genetic maps in breeding programs.
authors.affiliation: Instituto Agronômico, Centro APTA Citros Sylvio Moreira, Cordeirópolis, SP, Brazil.
authors.forename: Valdenice M
authors.initials: VM
authors.lastname: Novelli
authors.forename: Mariângela
authors.initials: M
authors.lastname: Cristofani-Yaly
authors.forename: Marinês
authors.initials: M
authors.lastname: Bastianel
authors.forename: Dario A
authors.initials: DA
authors.lastname: Palmieri
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Machado
mesh_terms: D001947:Breeding / Q000379:methods; D002957:Citrus / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D018745:Genome, Plant; D015698:Genomic Library; D018895:Microsatellite Repeats / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers