title: Anopheles arabiensis sperm production after genetic manipulation, dieldrin treatment, and irradiation.
abstract: The use of the sterile insect technique relies on the release of sterilized mass-reared male insects which, before field releases, endure several unnatural treatments. In the case of Anopheles arabiensis (Patton) sterile insect technique program in Sudan, the genetic background of the original strain was first changed to create a genetic sexing strain that is based on a dieldrin-resistant mutation. Secondly, the eggs of the genetic sexing strain require treatment with dieldrin to allow complete elimination of female L1 larvae to enable the release of males only. Finally, male mosquitoes receive an irradiation dose of 70 Gy as pupae for sterilization. The effects of these treatments on sperm production were tested separately and in combination. Irradiation alone significantly decreased the initial sperm number and prevented new sperm production. However, the dieldrin treatment, aimed at eliminating females, appears to have an unexpected radioprotectant effect.
authors.affiliation: Insect Pest Control Laboratory, Joint Food and Agriculture Organization/International Atomic Energy Agency (FAO/IAEA), Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramerstrasse 5, PO Box 100, A-1400 Vienna, Austria. d.damiens@iaea.org
authors.forename: D
authors.initials: D
authors.lastname: Damiens
authors.forename: M J B
authors.initials: MJ
authors.lastname: Vreysen
authors.forename: J R L
authors.initials: JR
authors.lastname: Gilles
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000502:physiology; D000852:Anopheles / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D004026:Dieldrin / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology*; D009032:Mosquito Control / Q000379:methods*; D010063:Ovum / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects* / Q000528:radiation effects*; D013242:Sterilization; D013397:Sudan
chemical_list: D007306:Insecticides; D004026:Dieldrin