title: Periodontal ligament stem cells regulate B lymphocyte function via programmed cell death protein 1.
abstract: Periodontal ligament stem cells (PDLSCs) have provided novel cell sources for tooth and periodontal tissue regeneration. Allogeneic PDLSCs can reconstruct periodontal ligament tissue that has been damaged by periodontal diseases and regulate T-cell immunity. However, the effect of PDLSCs on B cells remains unknown. Here, we treated periodontitis in a miniature pig model using allogeneic PDLSCs and showed a reduction in humoral immunity in the animals. When cocultured with normal B cells, human PDLSCs (hPDLSCs) had similar effects as bone marrow mesenchymal stem cells in suppressing B cell proliferation, differentiation, and migration, while intriguingly, hPDLSCs increased B cell viability by secreting interleukin-6. Mechanistically, hPDLSCs suppressed B cell activation through cell-to-cell contact mostly mediated by programmed cell death protein 1 and programmed cell death 1 ligand 1. Our data revealed a previously unrecognized function of PDLSCs in regulating humoral immune responses, which may represent a novel therapeutic strategy for immune-related disorders.
authors.affiliation: Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Ousheng
authors.initials: O
authors.lastname: Liu
authors.forename: Junji
authors.initials: J
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.forename: Dayong
authors.initials: D
authors.lastname: Liu
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Fan
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Wanjun
authors.initials: W
authors.lastname: Chen
authors.forename: Yaozhong
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhangui
authors.initials: Z
authors.lastname: Tang
authors.forename: Songlin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology*; D016923:Cell Death / Q000502:physiology; D002454:Cell Differentiation / Q000276:immunology / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D005260:Female; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D023421:Models, Animal; D010513:Periodontal Ligament / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D010518:Periodontitis / Q000473:pathology; D033581:Stem Cell Transplantation / Q000379:methods; D013552:Swine; D013556:Swine, Miniature
chemical_list: D051017:Apoptosis Regulatory Proteins
keywords: B lymphocyte; Humoral immunity; Immunoregulation; Mesenchymal stem cells; Periodontal ligament stem cells; Periodontitis; Programmed cell death protein 1