title: The impact of cystic fibrosis on the immunologic profile of pediatric patients.
abstract: OBJECTIVE
  To compare the immunologic state of 44 pediatric patients with cystic fibrosis (CF) with a control group consisting of 16 healthy individuals.
  
  
  METHODS
  CF patients aged 3 to 12 years with moderate to good clinical score were selected for the study. Erythrocytic glutathione, production of reactive oxygen species, cytokines (TNF-a, IFN-g, IL-8, IL-6, IL-10) in peripheral blood mononuclear cells cultures under spontaneous and BCG- or PHA-stimulated conditions, serum concentrations of TGF-b2, IgA, IgG, IgM, IgE, and salivary IgA were evaluated.
  
  
  RESULTS
  The spontaneous production of TNF-a, IL-6, and IL-10, the PHA-stimulated production of IL-6, and the serum TGF-b2, IgA, and IgG were increased in samples from CF patients. Healthy subjects had a higher production of TNF-a in response to BCG.
  
  
  CONCLUSIONS
  Although CF patients appeared clinically stable, the results of their peripheral blood examinations demonstrated an impact on the immune system.
authors.affiliation: Department of Food and Nutrition, School of Food Engineering, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil. dani_miotto@yahoo.com.br
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Bernardi
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Ribeiro
authors.forename: Tais N
authors.initials: TN
authors.lastname: Mazzola
authors.forename: Maria M S
authors.initials: MM
authors.lastname: Vilela
authors.forename: Valdemiro C
authors.initials: VC
authors.lastname: Sgarbieri
mesh_terms: D016022:Case-Control Studies; D018929:Cell Culture Techniques; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003550:Cystic Fibrosis / Q000097:blood / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005978:Glutathione / Q000096:biosynthesis*; D006801:Humans; D007136:Immunoglobulins / Q000097:blood*; D018836:Inflammation Mediators / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D015166:Monitoring, Immunologic; D017382:Reactive Oxygen Species / Q000097:blood; D012463:Saliva / Q000276:immunology; D053781:Transforming Growth Factor beta2 / Q000097:blood
chemical_list: D016207:Cytokines; D007136:Immunoglobulins; D018836:Inflammation Mediators; D017382:Reactive Oxygen Species; D053781:Transforming Growth Factor beta2; D005978:Glutathione