title: Hyaluronic acid/chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes.
abstract: Binding, stabilizing and promoting cellular uptake of siRNA are all critical efforts in creating matrices for the localized delivery of siRNA molecules to target cells. In this study, we describe the generation of chitosan imidazole/siRNA nanoplexes (NPs) embedded in nano scope polyelectrolyte multilayers (PEMs) composed of hyaluronic acid and chitosan for sustained and localized drug delivery. Regular PEM build-up, successful integration of NPs and controlled release under physiological conditions were shown. Biological efficacy was evaluated in neuronal cell culture concerning cell adhesion, viability, NPs uptake and gene silencing. The additionally shown biological functionalization of neuronal implants possesses potential for future applications in the field of regenerative medicine and treatment of spinal cord injuries.
authors.affiliation: NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Hartmann
authors.forename: Susanne
authors.initials: S
authors.lastname: Hossfeld
authors.forename: Burkhard
authors.initials: B
authors.lastname: Schlosshauer
authors.forename: Ursula
authors.initials: U
authors.lastname: Mittnacht
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Pêgo
authors.forename: Martin
authors.initials: M
authors.lastname: Dauner
authors.forename: Michael
authors.initials: M
authors.lastname: Doser
authors.forename: Dieter
authors.initials: D
authors.lastname: Stoll
authors.forename: Rumen
authors.initials: R
authors.lastname: Krastev
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D055785:Gene Knockdown Techniques; D006820:Hyaluronic Acid / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry; D009474:Neurons; D016716:PC12 Cells; D019736:Prostheses and Implants; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D020742:rhoA GTP-Binding Protein / Q000235:genetics
chemical_list: D007093:Imidazoles; D034741:RNA, Small Interfering; C029899:imidazole; D006820:Hyaluronic Acid; D048271:Chitosan; D020742:rhoA GTP-Binding Protein