title: Human parvovirus B19 NS1 protein aggravates liver injury in NZB/W F1 mice.
abstract: Human parvovirus B19 (B19) has been associated with a variety of diseases. However, the influence of B19 viral proteins on hepatic injury in SLE is still obscure. To elucidate the effects of B19 viral proteins on livers in SLE, recombinant B19 NS1, VP1u or VP2 proteins were injected subcutaneously into NZB/W F1 mice, respectively. Significant expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were detected in NZB/W F1 mice receiving B19 NS1 as compared to those mice receiving PBS. Markedly hepatocyte disarray and lymphocyte infiltration were observed in livers from NZB/WF 1 mice receiving B19 NS1 as compared to those mice receiving PBS. Additionally, significant increases of Tumor Necrosis Factor -α (TNF-α), TNF-α receptor, IκB kinase -α (IKK-α), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (IκB) and nuclear factor-kappa B (NF-κB) were detected in livers from NZB/W F1 mice receiving B19 NS1 as compared to those mice receiving PBS. Accordingly, significant increases of matrix metalloproteinase-9 (MMP9) and U-plasminogen activator (uPA) were also detected in livers from NZB/W F1 mice receiving B19 NS1 as compared to those mice receiving PBS. Contrarily, no significant variation on livers from NZB/W F1 mice receiving B19 VP1u or VP2 was observed as compared to those mice receiving PBS. These findings firstly demonstrated the aggravated effects of B19 NS1 but not VP1u or VP2 protein on hepatic injury and provide a clue in understanding the role of B19 NS1 on hepatic injury in SLE.
authors.affiliation: Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Chun-Chou
authors.initials: CC
authors.lastname: Tsai
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Chiu
authors.forename: Jeng-Dong
authors.initials: JD
authors.lastname: Hsu
authors.forename: Huai-Sheng
authors.initials: HS
authors.lastname: Hsu
authors.forename: Bor-Show
authors.initials: BS
authors.lastname: Tzang
authors.forename: Tsai-Ching
authors.initials: TC
authors.lastname: Hsu
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004731:Endotoxins / Q000378:metabolism; D005260:Female; D022781:Hepatocytes / Q000166:cytology; D051550:I-kappa B Kinase / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000821:virology*; D008180:Lupus Erythematosus, Systemic / Q000503:physiopathology*; D008214:Lymphocytes / Q000166:cytology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008814:Mice, Inbred NZB; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D016366:Open Reading Frames; D016732:Parvovirus B19, Human; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D004731:Endotoxins; D020672:I-kappa B Proteins; C106202:NS1 protein, parvovirus; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D017361:Viral Nonstructural Proteins; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D051550:I-kappa B Kinase; D014568:Urokinase-Type Plasminogen Activator; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse
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