title: New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering.
abstract: Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique. It was found that ChSs prepared with 200 mg chitosan powder (ChSs200) had well-structured three-dimensional architecture with high porosity and good retentive form without brittleness. In addition, it was shown that the number of osteoblast-like cells MC3T3-E1 proliferated on desalinated ChSs200 was larger than that on the non-desalinated ChSs200. CA-ChSs were fabricated by adding 100 mg carbonate apatite (CA) to 200 mg chitosan gels followed by freeze-drying (CA100ChSs200). SEM observation revealed that CA100ChSs200 had favorable three- dimensional porous structures. The number of living cells increased more rapidly on CA100ChSs200 prepared with different amounts of CA than on ChSs. ALP activity significantly increased after day 14 and reached a plateau after day 21 in ChSs200 and CA100ChSs200. It was concluded that newly developed CA100ChSs200 may be a possible scaffold material for tissue engineering.
authors.affiliation: Department of Advanced Prosthodontics, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Maretaningtias Dwi
authors.initials: MD
authors.lastname: Ariani
authors.forename: Ayumu
authors.initials: A
authors.lastname: Matsuura
authors.forename: Isao
authors.initials: I
authors.lastname: Hirata
authors.forename: Takayasu
authors.initials: T
authors.lastname: Kubo
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Akagawa
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D001861:Bone Regeneration / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D048271:Chitosan / Q000737:chemistry*; D005612:Freeze Drying; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology; D018583:Pliability; D016062:Porosity; D011208:Powders; D011474:Prosthesis Design; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
chemical_list: D001031:Apatites; D011208:Powders; C030782:carboapatite; D048271:Chitosan; D000469:Alkaline Phosphatase