title: An archaeal homolog of proteasome assembly factor functions as a proteasome activator.
abstract: Assembly of the eukaryotic 20S proteasome is an ordered process involving several proteins operating as proteasome assembly factors including PAC1-PAC2 but archaeal 20S proteasome subunits can spontaneously assemble into an active cylindrical architecture. Recent bioinformatic analysis identified archaeal PAC1-PAC2 homologs PbaA and PbaB. However, it remains unclear whether such assembly factor-like proteins play an indispensable role in orchestration of proteasome subunits in archaea. We revealed that PbaB forms a homotetramer and exerts a dual function as an ATP-independent proteasome activator and a molecular chaperone through its tentacle-like C-terminal segments. Our findings provide insights into molecular evolution relationships between proteasome activators and assembly factors.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku Nagoya, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kumoi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Satoh
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Murata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hiromoto
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Mizushima
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Masanori
authors.initials: M
authors.lastname: Noda
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yagi
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D001105:Archaea / Q000378:metabolism*; D019843:Archaeal Proteins / Q000378:metabolism*; D019143:Evolution, Molecular; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D019843:Archaeal Proteins; D046988:Proteasome Endopeptidase Complex
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