title: Brief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells.
abstract: Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings toward the development of novel diagnostics and therapies with direct clinical relevance. This study demonstrates that the cluster of differentiation genes CD24 and CD44 are differentially expressed across LGR5 positive "active" stem cells as well as HOPX positive "facultative" stem cells. Fluorescence-activated cell sorting enables differential enrichment of LGR5 (CD24-/CD44+) and HOPX (CD24+/CD44+) cells for gene expression analysis and culture. These findings provide the fundamental methodology and basic cell surface signature necessary for isolating and studying intestinal stem cell populations in human physiology and disease.
authors.affiliation: Department of Medicine Division of Gastroenterology and Hepatology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA; Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: Adam D
authors.initials: AD
authors.lastname: Gracz
authors.forename: Megan K
authors.initials: MK
authors.lastname: Fuller
authors.forename: Fengchao
authors.initials: F
authors.lastname: Wang
authors.forename: Linheng
authors.initials: L
authors.lastname: Li
authors.forename: Matthias
authors.initials: M
authors.lastname: Stelzner
authors.forename: James C Y
authors.initials: JC
authors.lastname: Dunn
authors.forename: Martin G
authors.initials: MG
authors.lastname: Martin
authors.forename: Scott T
authors.initials: ST
authors.lastname: Magness
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D051927:CD24 Antigen / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007422:Intestines / Q000166:cytology*; D008875:Middle Aged; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D051927:CD24 Antigen; D018960:Hyaluronan Receptors
keywords: Cell culture; Cell surface markers; Flow cytometry; Fluorescence-activated cell sorting; Tissue-specific stem cells
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