title: Iron indices in bottlenose dolphins (Tursiops truncatus).
abstract: Bottlenose dolphins can have iron overload (that is, hemochromatosis), and managed populations of dolphins may be more susceptible to this disease than are wild dolphins. Serum iron, total iron-binding capacity (TIBC), transferrin saturation, and ferritin were measured in 181 samples from 141 dolphins in 2 managed collections and 2 free-ranging populations. Although no iron indices increased with age among free-ranging dolphins, ferritin increased with age in managed collections. Dolphins from managed collections had higher iron, ferritin, and transferrin saturation values than did free-ranging dolphins. Dolphins with high serum iron (exceeding 300 μg/dL) were more likely to have elevated ferritin but not ceruloplasmin or haptoglobin, demonstrating that high serum levels of iron are due to a true increase in total body iron. A time-series study of 4 dolphins with hemochromatosis that were treated with phlebotomy demonstrated significant decreases in serum ferritin, iron, and TIBC between pre- and posttreatment samples; transferrin saturation initially fell but returned to prephlebotomy levels by 6 mo after treatment. Compared with those in managed collections, wild dolphins were 15 times more likely to have low serum iron (100 μg/dL or less), and this measure was associated with lower haptoglobin. In conclusion, bottlenose dolphins in managed collections are more likely to have greater iron stores than are free-ranging dolphins. Determining why this situation occurs among some dolphin populations and not others may improve the treatment of hemochromatosis in dolphins and provide clues to causes of nonhereditary hemochromatosis in humans.
authors.affiliation: Mystic Aquarium, a Division of Sea Research Foundation, Mystic, CT, USA. LMazzaro@searesearch.org
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Mazzaro
authors.forename: Shawn P
authors.initials: SP
authors.lastname: Johnson
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Fair
authors.forename: Greg
authors.initials: G
authors.lastname: Bossart
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Carlin
authors.forename: Eric D
authors.initials: ED
authors.lastname: Jensen
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Smith
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Andrews
authors.forename: Patricia S
authors.initials: PS
authors.lastname: Chavey
authors.forename: Stephanie
authors.initials: S
authors.lastname: Venn-Watson
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000835:Animals, Wild / Q000097:blood*; D000836:Animals, Zoo / Q000097:blood*; D050681:Bottle-Nosed Dolphin / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005293:Ferritins / Q000097:blood; D006242:Haptoglobins / Q000032:analysis; D006432:Hemochromatosis / Q000097:blood / Q000662:veterinary*; D007501:Iron / Q000097:blood*; D033862:Iron-Binding Proteins / Q000378:metabolism*; D018962:Phlebotomy / Q000662:veterinary; D013053:Spectrophotometry / Q000662:veterinary; D014168:Transferrin / Q000378:metabolism*
chemical_list: D006242:Haptoglobins; D033862:Iron-Binding Proteins; D014168:Transferrin; D005293:Ferritins; D007501:Iron
references.citation: J Am Vet Med Assoc. 2006 Jan 1;228(1):104-8
references.pmid: 16426180
references.citation: Am J Primatol. 2008 Jul;70(7):629-33
references.pmid: 18366107
references.citation: Mar Pollut Bull. 2012 Jan;64(1):66-74
references.pmid: 22118898
references.citation: Primates. 2002 Jul;43(3):179-90
references.pmid: 12145399
references.citation: Acta Haematol. 2002;107(3):145-9
references.pmid: 11978935
references.citation: J Wildl Dis. 2006 Apr;42(2):447-54
references.pmid: 16870874
references.citation: Vet Clin Pathol. 1998;27(1):11-14
references.pmid: 12075554
references.citation: J Wildl Dis. 2008 Apr;44(2):318-30
references.pmid: 18436664
references.citation: J Endotoxin Res. 2005;11(5):267-80
references.pmid: 16262999
references.citation: J Zoo Wildl Med. 2005 Jun;36(2):212-21
references.pmid: 17323561
references.citation: Sci Total Environ. 2010 Mar 1;408(7):1577-97
references.pmid: 20096443
references.citation: Gen Comp Endocrinol. 2011 Jan 1;170(1):193-9
references.pmid: 20951701
references.citation: Anal Biochem. 1972 Jul;48(1):266-78
references.pmid: 5041404
references.citation: J Am Vet Med Assoc. 2007 Aug 15;231(4):596-601
references.pmid: 17696863
references.citation: Nat Genet. 1996 Aug;13(4):399-408
references.pmid: 8696333
references.citation: Mod Pathol. 2007 Feb;20 Suppl 1:S31-9
references.pmid: 17486050
references.citation: J Vet Intern Med. 1994 Mar-Apr;8(2):105-11
references.pmid: 8046672
references.citation: J Zoo Wildl Med. 2006 Mar;37(1):49-52
references.pmid: 17312812
references.citation: J Am Vet Med Assoc. 2008 Jul 15;233(2):299-307
references.pmid: 18627240
references.citation: Eur J Endocrinol. 2006 Feb;154(2):333-40
references.pmid: 16452549
references.citation: J Zoo Wildl Med. 2012 Sep;43(3 Suppl):S35-47
references.pmid: 23156704
references.citation: J Am Vet Med Assoc. 2009 Jul 15;235(2):194-200
references.pmid: 19601742