title: [Development of real-time fluorescent quantitative PCR method for detecting Bombyx mori cytoplasmic polyhedrosis virus].
abstract: A pair of specific primers were designed according to the published 118 bp conserved sequence of polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) in GenBank and a serial dilutions of a recombinant plasmid were prepared and used to generate standard curves, to establish a real-time fluorescent quantitative PCR method for detection of BmCPV. The results showed that the linear relationship between virus concentration (X) and Ct (Y) was Y = -3. 582 lgX + 38.748 with the correlation coefficient R2 = 0999. The method was very sensitive, specific and reproducible. It can be applied in the rapid detection of BmCPV and the prevalence investigation of this disease.
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China. wp4114@163.com
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: He-Ying
authors.initials: HY
authors.lastname: Qian
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D005453:Fluorescence; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012087:Reoviridae / Q000235:genetics / Q000302:isolation & purification*