title: SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.
abstract: SOX2 was reported to promote metastasis in various tumor tissues; however the underlying mechanisms remain elusive. Here, we disclosed that SOX2 improves metastasis of breast and prostate cancer cells by promoting epithelial-to-mesenchymal transition (EMT) through WNT/β-catenin, but not TGF-β or Snail1 signaling. Dual luciferase assay and chromatin immunoprecipitation revealed activation and binding of SOX2 on promoter region of β-catenin. In addition, SOX2 affects the protein expression levels of DKK3, DVL1 and DVL3, which are regulators or downstream molecules of WNT signaling. Taken together, our findings demonstrated β-catenin as one of vital downstream molecules that mediate the EMT induced by SOX2.
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Li
authors.forename: Yingxi
authors.initials: Y
authors.lastname: Xu
authors.forename: Yanan
authors.initials: Y
authors.lastname: Chen
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.forename: Xianpei
authors.initials: X
authors.lastname: Jia
authors.forename: Tongchao
authors.initials: T
authors.lastname: Sun
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiru
authors.initials: X
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D018925:Chemokines; D000072261:Dishevelled Proteins; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010750:Phosphoproteins / Q000096:biosynthesis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C495270:CTNNB1 protein, human; D018925:Chemokines; C498624:DKK3 protein, human; C000606578:DVL1 protein, human; C000606584:DVL3 protein, human; D000072261:Dishevelled Proteins; C000606579:Dvl1 protein, mouse; C000606585:Dvl3 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D010750:Phosphoproteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin