title: Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns.
abstract: METHODS
  IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain.
  
  
  RESULTS
  Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein.
  
  
  CONCLUSIONS
  We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.
authors.affiliation: Department of Allergy, IIS-Fundación Jiménez Díaz, Madrid, Spain. marina.perezgordo@ceu.es
authors.forename: Marina
authors.initials: M
authors.lastname: Perez-Gordo
authors.forename: Carlos
authors.initials: C
authors.lastname: Pastor-Vargas
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Ludmilla
authors.initials: L
authors.lastname: Bardina
authors.forename: Barbara
authors.initials: B
authors.lastname: Cases
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Ibáñez
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
authors.forename: Javier
authors.initials: J
authors.lastname: Cuesta-Herranz
authors.forename: Hugh A
authors.initials: HA
authors.lastname: Sampson
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000097:blood / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D048389:Gadus morhua / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D008969:Molecular Sequence Data; D010320:Parvalbumins / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D013030:Spain; D044465:White People; D055815:Young Adult
chemical_list: D000485:Allergens; D000941:Antigens; D000939:Epitopes; D010320:Parvalbumins; D007073:Immunoglobulin E
keywords: Cod parvalbumin; Epitope mapping; Fish allergy; Food allergy; Peptide microarray