title: LC-QTOF/MS metabolomic profiles in human plasma after a 5-week high dietary fiber intake.
abstract: The objective was to investigate the alterations of plasma metabolome profiles to identify exposure and effect markers of dietary fiber intake. Subjects (n = 25) aged 58.6 (1.1) years (mean and SD) with a body mass index of 26.6 (0.5) kg/m(2) were given a high fiber (HF) and a low fiber (LF) diet, in a 5-week randomized controlled crossover intervention. The HF diet consisted of oat bran, rye bran, and sugar beet fiber incorporated into test food products, whereas the LF diet was made of equivalent food products to the HF diet, but without adding fibers. Blood plasma samples were collected at the start and end of each intervention period and analyzed by LC-QTOF/MS. In total, 6 features in positive mode and 14 features in negative mode were significantly different between the HF and the LF diet (p < 0.01, q < 0.05). Two markers, 2,6-dihydroxybenzoic acid and 2-aminophenol sulfate, were increased after HF diet, along with a tentatively identified saponin derived from oat avenacosides. The untargeted metabolomics approach enabled the identification of two new markers of dietary fiber intake in human plasma. Further studies will be needed to verify if these markers could serve as compliance markers of fiber intake.
authors.affiliation: Biomedical Nutrition, Pure and Applied Biochemistry, Center for Applied Life Sciences, Department of Chemistry, Lund University, Lund, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Johansson-Persson
authors.forename: Thaer
authors.initials: T
authors.lastname: Barri
authors.forename: Matilda
authors.initials: M
authors.lastname: Ulmius
authors.forename: Gunilla
authors.initials: G
authors.lastname: Onning
authors.forename: Lars Ove
authors.initials: LO
authors.lastname: Dragsted
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D018592:Cross-Over Studies; D004043:Dietary Fiber / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D004043:Dietary Fiber; D020543:Proteome