title: Aurora A is involved in central spindle assembly through phosphorylation of Ser 19 in P150Glued.
abstract: Knowledge of Aurora A kinase functions is limited to premetaphase events, particularly centrosome maturation, G2/M transition, and mitotic spindle assembly. The involvement of Aurora A in events after metaphase has only been suggested because appropriate experiments are technically difficult. We report here the design of the first human Aurora A kinase (as-AurA) engineered by chemical genetics techniques. This kinase is fully functional biochemically and in cells, and is rapidly and specifically inhibited by the ATP analogue 1-Naphthyl-PP1 (1-Na-PP1). By treating cells exclusively expressing the as-AurA with 1-Na-PP1, we discovered that Aurora A is required for central spindle assembly in anaphase through phosphorylation of Ser 19 of P150Glued. This paper thus describes a new Aurora A function that takes place after the metaphase-to-anaphase transition and a new powerful tool to search for and study new Aurora A functions.
authors.affiliation: Unité Mixte de Recherche 6290, Centre National de la Recherche Scientifique, F-35043 Rennes, France. david.reboutier@univ-rennes1.fr
authors.forename: David
authors.initials: D
authors.lastname: Reboutier
authors.forename: Marie-Bérengère
authors.initials: MB
authors.lastname: Troadec
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Cremet
authors.forename: Lucie
authors.initials: L
authors.lastname: Chauvin
authors.forename: Vincent
authors.initials: V
authors.lastname: Guen
authors.forename: Patrick
authors.initials: P
authors.lastname: Salaun
authors.forename: Claude
authors.initials: C
authors.lastname: Prigent
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000705:Anaphase / Q000187:drug effects / Q000502:physiology*; D064127:Aurora Kinases; D002460:Cell Line; D000072159:Dynactin Complex; D006801:Humans; D008677:Metaphase / Q000187:drug effects / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*
chemical_list: D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D012694:Serine; D000255:Adenosine Triphosphate; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases
references.citation: Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):6998-7003
references.pmid: 19369198
references.citation: Chem Biol. 1998 Feb;5(2):91-101
references.pmid: 9495830
references.citation: J Biol Chem. 2003 Jan 24;278(4):2236-41
references.pmid: 12426316
references.citation: Mol Cell Biol. 2005 Jul;25(14):5789-800
references.pmid: 15987997
references.citation: Curr Opin Cell Biol. 2008 Feb;20(1):77-84
references.pmid: 18249108
references.citation: Curr Biol. 2005 Dec 20;15(24):2249-55
references.pmid: 16360686
references.citation: Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4383-8
references.pmid: 17360533
references.citation: Genes Cells. 2002 Nov;7(11):1173-82
references.pmid: 12390251
references.citation: J Biol Chem. 1997 May 23;272(21):13766-71
references.pmid: 9153231
references.citation: Curr Biol. 2002 Apr 16;12(8):640-7
references.pmid: 11967150
references.citation: J Proteome Res. 2009 Nov;8(11):4943-53
references.pmid: 19799413
references.citation: Mol Cell Biochem. 2003 Jan;243(1-2):123-31
references.pmid: 12619897
references.citation: J Cell Sci. 2006 Nov 1;119(Pt 21):4431-41
references.pmid: 17046997
references.citation: ACS Chem Biol. 2010 Jun 18;5(6):563-76
references.pmid: 20426425
references.citation: Mol Cancer. 2011 Oct 19;10:131
references.pmid: 22011530
references.citation: Dev Cell. 2006 Apr;10(4):509-20
references.pmid: 16580995
references.citation: Sci Signal. 2011 Jun 28;4(179):rs5
references.pmid: 21712546
references.citation: J Cell Sci. 1998 Mar;111 ( Pt 5):557-72
references.pmid: 9454730
references.citation: Cancer Cell. 2003 Jan;3(1):51-62
references.pmid: 12559175
references.citation: Mol Biol Cell. 2002 Oct;13(10):3627-45
references.pmid: 12388762
references.citation: Curr Biol. 1998 Feb 26;8(5):257-66
references.pmid: 9501066
references.citation: J Cell Biol. 2010 Oct 18;191(2):259-67
references.pmid: 20937700
references.citation: J Cell Biol. 1995 Dec;131(6 Pt 1):1507-16
references.pmid: 8522607
references.citation: J Biol Chem. 2002 Mar 22;277(12):10719-26
references.pmid: 11790771
references.citation: J Cell Biol. 2005 Sep 26;170(7):1047-55
references.pmid: 16172205
references.citation: Cancer Res. 2002 Jul 15;62(14):4115-22
references.pmid: 12124350
references.citation: Cell Cycle. 2006 Nov;5(22):2555-6
references.pmid: 17172841
references.citation: J Cell Biol. 2001 Dec 24;155(7):1109-16
references.pmid: 11748251
references.citation: J Cell Biol. 2012 Apr 2;197(1):19-26
references.pmid: 22451695
references.citation: Biochem Biophys Res Commun. 2011 Apr 15;407(3):610-4
references.pmid: 21426902
references.citation: J Cell Biol. 2003 Feb 3;160(3):297-301
references.pmid: 12551954
references.citation: Mol Cell Biol. 2007 Jan;27(1):352-67
references.pmid: 17060449
references.citation: J Biol Chem. 2001 Feb 23;276(8):5821-8
references.pmid: 11085985
references.citation: J Cell Biol. 2002 Feb 4;156(3):437-51
references.pmid: 11827981
references.citation: J Biol Chem. 2005 Oct 14;280(41):35051-61
references.pmid: 16081410
references.citation: J Cell Biol. 2011 Dec 26;195(7):1103-13
references.pmid: 22184196
references.citation: Genes Dev. 1998 May 15;12(10):1483-94
references.pmid: 9585508
references.citation: Nature. 2008 Sep 4;455(7209):119-23
references.pmid: 18615013
references.citation: Neuron. 2005 Apr 7;46(1):13-21
references.pmid: 15820690
references.citation: Mol Biol Cell. 2008 Jul;19(7):2752-65
references.pmid: 18434591
references.citation: Biochem Biophys Res Commun. 2006 Mar 3;341(1):261-5
references.pmid: 16431218
references.citation: Nat Rev Mol Cell Biol. 2009 Jan;10(1):9-20
references.pmid: 19197328
references.citation: J Cell Biol. 2010 May 17;189(4):651-9
references.pmid: 20479466
references.citation: Biochemistry. 2007 Sep 11;46(36):10287-95
references.pmid: 17705509
references.citation: J Cell Biol. 2001 Feb 19;152(4):669-82
references.pmid: 11266459
references.citation: J Cell Sci. 2009 May 1;122(Pt 9):1334-41
references.pmid: 19351716
references.citation: Curr Biol. 2006 Apr 18;16(8):743-54
references.pmid: 16631581
references.citation: J Cell Biol. 2002 Jul 22;158(2):305-19
references.pmid: 12119357
references.citation: Pediatr Blood Cancer. 2010 Jul 15;55(1):26-34
references.pmid: 20108338
references.citation: Mol Cell. 2005 Aug 19;19(4):449-60
references.pmid: 16109370
references.citation: Dev Biol. 2008 May 15;317(2):523-30
references.pmid: 18395707
references.citation: J Biol Chem. 2003 Dec 19;278(51):51786-95
references.pmid: 14523000
references.citation: Biochemistry. 2008 Jan 15;47(2):779-86
references.pmid: 18081319
references.citation: Oncogene. 2008 Nov 20;27(51):6539-49
references.pmid: 18663358
references.citation: Dev Cell. 2002 Jan;2(1):41-54
references.pmid: 11782313
references.citation: Curr Biol. 1999 Apr 22;9(8):425-8
references.pmid: 10226031
references.citation: Nat Rev Cancer. 2005 Jan;5(1):42-50
references.pmid: 15630414
references.citation: J Biol Chem. 2011 Aug 26;286(34):30097-106
references.pmid: 21705324
references.citation: J Cell Sci. 2004 May 15;117(Pt 12):2523-31
references.pmid: 15128871
references.citation: Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):4106-11
references.pmid: 17360485