title: Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14.
abstract: DING proteins form an emergent family of proteins consisting of an increasing number of homologues that have been identified in all kingdoms of life. They belong to the superfamily of phosphate-binding proteins and exhibit a high affinity for phosphate. In eukaryotes, DING proteins have been isolated by virtue of their implication in several diseases and biological processes. Some of them are potent inhibitors of HIV-1 replication/transcription, raising the question of their potential involvement in the human defence system. Recently, a protein from Pseudomonas aeruginosa strain PA14, named PA14DING or LapC, belonging to the DING family has been identified. The structure of PA14DING, combined with detailed biochemical characterization and comparative analysis with available DING protein structures, will be helpful in understanding the structural determinants implicated in the inhibition of HIV-1 by DING proteins. Here, the expression, purification and crystallization of PA14DING and the collection of X-ray data to 1.9 Å resolution are reported.
authors.affiliation: Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 27 Boulevard Jean Moulin, 13385 Marseille CEDEX 5, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Djeghader
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gotthard
authors.forename: Andrew
authors.initials: A
authors.lastname: Suh
authors.forename: Daniel
authors.initials: D
authors.lastname: Gonzalez
authors.forename: Ken
authors.initials: K
authors.lastname: Scott
authors.forename: Mikael
authors.initials: M
authors.lastname: Elias
authors.forename: Eric
authors.initials: E
authors.lastname: Chabriere
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D028044:Phosphate-Binding Proteins / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D028044:Phosphate-Binding Proteins
keywords: DING proteins; HIV; antivirals; phosphate-binding proteins; virulence factors
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