title: A 5'-flanking region polymorphism in toll-like receptor 4 is associated with gastric cancer in a Chinese population.
abstract: OBJECTIVE
  Inflammation induced by H.pylori colonization in the stomach is related to the development of gastric cancer and the genetic variations of the genes involved in the immune responses modify the host response to the infection. The aim of this study was to evaluate whether polymorphisms in the toll-like receptor 4 (TLR4) gene, a key regulator of both innate and adaptive immunity, were related to the susceptibility to gastric cancer in a Chinese population.
  
  
  METHODS
  Two variations in the 5'-flanking region of TLR4 (rs1927914 T > C and rs10759932 T > C) were genotyped by using the PCR-restriction fragment length polymorphism (RFLP) assay in a case-control study of 1,053 incident gastric cancer cases and 1,100 cancer-free controls in a Chinese population.
  
  
  RESULTS
  Individuals carrying the C allele of rs10759932 had a significantly reduced risk of gastric cancer (adjusted OR = 0.81; 95%CI = 0.67-0.96), compared with the wild-type homozygote (TT), and the protective effect was not significantly different among subgroups stratified by age, sex, smoking, drinking and H.pylori infection status (P for heterogeneity > 0.05). No significant association was observed between rs1927914 and gastric cancer risk in this study population.
  
  
  CONCLUSIONS
  The T to C allele substitution of rs10759932 may play a protective role in gastric carcinogenesis in a Chinese population. Large studies with different ethnic populations are warranted to confirm these findings.
authors.affiliation: Department of Epidemiology and Biostatistics, Cancer Center, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.forename: Guangfu
authors.initials: G
authors.lastname: Jin
authors.forename: Hanze
authors.initials: H
authors.lastname: Zhang
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhaolai
authors.initials: Z
authors.lastname: Hua
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Hu
authors.forename: Yaochu
authors.initials: Y
authors.lastname: Xu
authors.forename: Hongbing
authors.initials: H
authors.lastname: Shen
keywords: gastric cancer; polymorphisms; susceptibility; toll-like receptor 4
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