title: Aluminium phosphide-induced genetic and oxidative damages in vitro: attenuation by Laurus nobilis L. leaf extract.
abstract: OBJECTIVE
  The present investigation was undertaken to assess the protective effect of Laurus nobilis leaf extract (LNE) against aluminum phosphide (AIP)-induced genotoxic and oxidative damages stress in cultured human blood cells in the presence of a metabolic activator (S9 mix).
  
  
  METHODS
  Sister chromatid exchange (SCE) and chromosome aberration (CA) assays were used to assess AlP-induced genotoxicity and to establish the protective effects of LNE. In addition, we determined total antioxidant capacity (TAC) and total oxidative status (TOS) levels in AlP and LNE treated cultures for biomonitoring the oxidative alterations.
  
  
  RESULTS
  There was significant increases (P < 0.05) in both SCE and CA frequencies of cultures treated with AlP as compared to controls. Our results also showed that AlP (58 mg/l) caused oxidative stress by altering TAC and TOS levels. However, co-application of LNE (25, 50, 100 and 200 mg/l) and AlP resulted in decreases of SCE, CA rates and TOS level and increases of TAC level as compared to the group treated with AlP alone.
  
  
  CONCLUSIONS
  The preventive role of LNE in alleviating AlP-induced DNA and oxidative damages was indicated for the first time in the present study.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Sciences, Erzurum Technical University, Erzurum, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Türkez
authors.forename: Başak
authors.initials: B
authors.lastname: Toğar
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D002478:Cells, Cultured / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D006801:Humans; D027442:Laurus / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010575:Pesticides / Q000633:toxicity*; D010720:Phosphines / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012854:Sister Chromatid Exchange / Q000187:drug effects
chemical_list: D017607:Aluminum Compounds; D010575:Pesticides; D010720:Phosphines; D010936:Plant Extracts; C001864:aluminum phosphide
keywords: Aluminum phosphide; Laurus nobilis leaf extract; genotoxicity; human blood culture; oxidative stress
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