title: Heat shock protein profiles on the protein and gene expression levels in olive flounder kidney infected with Streptococcus parauberis.
abstract: Heat shock proteins (HSPs) have been observed in cells exposed to a variety of stresses, including infectious pathogens. This study used a label-free, quantitative proteomic approach and transcriptional gene expression analysis to investigate infection-related HSP proteins and their encoding genes in whole kidneys from olive flounder (Paralichthys olivaceus). During Streptococcus parauberis infection in the flounder, the genes encoding Hsp10, Hsp40A4, Hsp40B6, Hsp40B11, Hsp60, Hsp70, glucose regulated protein 78 (Grp78), Hsp90α, Hsp90β and Grp94 were induced, and the protein levels of Hsp60, Hsp70, Hsp90α, Hsp90β and Grp94 were differentially regulated over time. Subsequent results also revealed that Hsp60, Hsp70, Hsp90α, Hsp90β and Grp94 appear to be the dominant and critical HSPs in olive flounder during bacterial infection. This is the first estimation of the differential involvement of HSPs in the immune response of olive flounder exposed to bacterial infection.
authors.affiliation: Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.
authors.forename: In Seok
authors.initials: IS
authors.lastname: Cha
authors.forename: Joseph
authors.initials: J
authors.lastname: Kwon
authors.forename: Seong Bin
authors.initials: SB
authors.lastname: Park
authors.forename: Ho Bin
authors.initials: HB
authors.lastname: Jang
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Nho
authors.forename: Young Kyu
authors.initials: YK
authors.lastname: Kim
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005432:Flounder; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D020539:Sequence Analysis, Protein / Q000662:veterinary; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000502:physiology; D053719:Tandem Mass Spectrometry / Q000662:veterinary
chemical_list: D029941:Fish Proteins; D006360:Heat-Shock Proteins