title: TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells.
abstract: It is widely accepted that somatic cells can be reprogrammed by a set of transcription factors to become embryonic stem cell-like: These reprogrammed cells, induced pluripotent stem cells (iPSCs), are nearly identical to embryonic stem cells (ESCs), because both have the capacity to self-renew and to form all cellular lineages of the body. Transcriptional differences between ESCs, iPSCs, and fibroblasts can be analyzed by quantitative PCR (qPCR) using TaqMan(®) Gene Expression assays, a widely used tool for rapid analysis of different cell types. In this chapter, we describe the OpenArray(®) platform which generates qPCR data from high-throughput instrumentation. We examined the gene signature profiles of ESCs, fibroblasts, and iPSCs with a TaqMan(®) OpenArray(®) Human Stem Cell Panel containing 631 TaqMan(®) Gene Expression assays that represent pathways involved in self-renewal, pluripotency, lineage patterning, transcriptional networks, stem cell differentiation, and development.
authors.affiliation: Genetic Analysis, Life Technologies, Forter City, CA, USA.
authors.forename: Sunali N
authors.initials: SN
authors.lastname: Patel
authors.forename: Yalei
authors.initials: Y
authors.lastname: Wu
authors.forename: Yun
authors.initials: Y
authors.lastname: Bao
authors.forename: Ricardo
authors.initials: R
authors.lastname: Mancebo
authors.forename: Janice
authors.initials: J
authors.lastname: Au-Young
authors.forename: Elena
authors.initials: E
authors.lastname: Grigorenko
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D015342:DNA Probes / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology; D052816:Foreskin / Q000166:cytology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger