title: Fishing and knockout of bioactive compounds using a combination of high-speed counter-current chromatography (HSCCC) and preparative HPLC for evaluating the holistic efficacy and interaction of the components of Herba Epimedii.
abstract: Due to the complex chemical compositions and pharmacological effects of traditional Chinese medicines, we developed a strategy based on fishing and knockout of bioactive compounds using a combination of high-speed counter-current chromatography (HSCCC) and preparative HPLC for evaluating the holistic activity and interaction of the components of Herba Epimedii. First, osteoblast target cell extraction was used for preliminary screening of the potential bioactive compounds of Herba Epimedii. Second, the bioactive compounds identified (epimedin A, epimedin B, epimedin C and icariin) were fished and knocked out using high-speed counter-current chromatography and preparative HPLC. Third, the bioactivity of resulting fractions was assessed by determining their influence on cell proliferation and differentiation, thereby allowing for an evaluation of their interaction.The pharmacodynamic contribution ratio of each bioactive compound to the efficacy of the herbal medicine could then be comprehensively and intuitively determined based on the spectra-activity correlations (VIP values) of the tested compositions using partial least-squares regression (PLS-R), through which the reliability of the screening and isolation of bioactive compounds by the target cell extraction technique were verified. The proposed strategy is a useful approach with potential application in other traditional Chinese medicines.
authors.affiliation: Tianjin Key Laboratory of TCM Chemistry and Analysis, School of Traditional Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Yubo
authors.initials: Y
authors.lastname: Li
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Kipletting Tanui
authors.forename: Liwen
authors.initials: L
authors.lastname: Han
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jia
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Zhang
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution; D034181:Epimedium / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D035261:Plant Components, Aerial; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D028321:Plant Preparations