title: High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array.
abstract: BACKGROUND
  Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable of performing high-throughput simultaneous detection of HPA-1 to -16, and the accuracy of many methods needs to be further enhanced.
  
  
  METHODS
  We have developed a new HPA-genotyping method for simultaneous detection of HPA-1 to -16 based on suspension array technology. A total of 216 samples from Chinese Han donors in Xi'an were genotyped using the developed method, and all the samples again were genotyped using polymerase chain reaction (PCR) sequence-based typing (PCR-SBT), which is considered the gold standard.
  
  
  RESULTS
  All 216 samples were successfully genotyped for HPA-1 to -16 using both our method and PCR-SBT. Results showed that the genotype and allele frequencies obtained using our method were fully consistent with those obtained using PCR-SBT.
  
  
  CONCLUSIONS
  Our method provides accurate, high-throughput, and simultaneous genotyping of HPA-1 to -16 and will serve as the foundation for large-scale clinical genotyping of HPAs and for the establishment of an HPA-typed PLT donor registry.
authors.affiliation: Department of Blood Transfusion, Xijing Hospital, Xi'an, China; Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China; State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China; Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China; Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China.
authors.forename: Qun-Xing
authors.initials: QX
authors.lastname: An
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Li
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Xu
authors.forename: Xian-Qing
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Bai
authors.forename: Zhong-Jun
authors.initials: ZJ
authors.lastname: Shao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D005838:Genotype; D057166:High-Throughput Screening Assays; D006801:Humans; D008969:Molecular Sequence Data; D017713:Platelet Transfusion; D016133:Polymerase Chain Reaction; D013535:Suspensions
chemical_list: D016824:Antigens, Human Platelet; D013535:Suspensions