title: Antiadhesive role of human amniotic fluid on peritoneal adhesion formation in a rat model. Experimental study.
abstract: OBJECTIVE
  To determine the role of human amniotic fluid (HAF) in preventing or reducing postoperative adhesions.
  
  
  METHODS
  Uterine horn adhesion model was carried out in 24 female Wistar rats. The animals were randomized into 4 groups: (1) control, (2) Ringer's lactate, (3) whole HAF, and (4) HAF depleted from cells and proteins. Adhesion grade and histologic findings of adhesion-carrying tissues were evaluated and groups were compared according to these parameters.
  
  
  RESULTS
  Rats treated with whole HAF had less adhesion grade when compared to the control group, but the difference was not statistically significant. On the other hand, centrifuged amniotic fluid treatment significantly reduced peritoneal adhesion grade, fibrosis and inflammation (p < 0.05).
  
  
  CONCLUSIONS
  Whole HAF seems to have no beneficial effect on peritoneal adhesion formation, but HAF depleted of protein and cells does have a positive effect on reducing adhesion formation.
authors.affiliation: Department of Obstetrics and Gynecology, Fatih University Faculty of Medicine, Vatan Street No. 81 Demetevler, Ankara, Turkey. denizhizli@yahoo.com
authors.forename: Deniz
authors.initials: D
authors.lastname: Hizli
authors.forename: Saynur
authors.initials: S
authors.lastname: Yilmaz
authors.forename: Nermin
authors.initials: N
authors.lastname: Kosus
authors.forename: Aydin
authors.initials: A
authors.lastname: Kosus
authors.forename: Hacer
authors.initials: H
authors.lastname: Haltas
authors.forename: Fatih
authors.initials: F
authors.lastname: Hizli
authors.forename: Hasan
authors.initials: H
authors.lastname: Kafali
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D001691:Biological Therapy; D004195:Disease Models, Animal; D005260:Female; D005355:Fibrosis / Q000150:complications / Q000517:prevention & control; D007249:Inflammation / Q000150:complications / Q000517:prevention & control; D010537:Peritoneum / Q000473:pathology* / Q000601:surgery*; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000150:complications / Q000473:pathology / Q000517:prevention & control*