title: Composition-dependent protein secretion and integrin level of osteoblastic cell on calcium silicate cements.
abstract: The purpose of this study was to investigate the responses of the human osteosarcoma cell line MG63 to calcium silicate cements with different Si/Ca molar ratios and different surface roughness. In particular, the study evaluated integrin subunit levels, phosphor-focal adhesion kinase (pFAK) levels and protein production at the cell attachment stage. The results indicated that the surface roughness (variations within a factor of 10) of the cements did not play a prominent role in cell attachment and proliferation, but the effect of composition was highlighted. Increased pFAK and total integrin levels and promoted cell attachment and cell cycle progression were observed upon an increase in cement Si content. Cement with a higher Si content was beneficial for collagen Type I (COL I) adsorption, COL I secretion, and αlibβ3 subintegrin expression, whereas cement with a higher Ca content increased fibronectin (FN) adsorption, FN secretion, and enhanced αvβ1 subintegrin levels. These results establish composition-dependent differences in integrin binding as a mechanism regulating cellular responses to biomaterial surfaces.
authors.affiliation: Institute of Oral Science, Chung Shan Medical University, Taichung City, 402, Taiwan.
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
authors.forename: Hsien-Chang
authors.initials: HC
authors.lastname: Chang
authors.forename: Shinn-Jyh
authors.initials: SJ
authors.lastname: Ding
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D003738:Dental Cements / Q000737:chemistry*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D017640:Silicates / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D003738:Dental Cements; D016023:Integrins; D017640:Silicates; D051416:Focal Adhesion Protein-Tyrosine Kinases; C031293:calcium silicate
keywords: calcium silicate; cell adhesion; integrin; protein adsorption; protein secretion