title: Potentiation of mycovirus transmission by zinc compounds via attenuation of heterogenic incompatibility in Rosellinia necatrix.
abstract: Heterogenic incompatibility is considered a defense mechanism against deleterious intruders such as mycovirus. Rosellinia necatrix shows strong heterogenic incompatibility. In the heterogenic incompatibility reaction, the approaching hyphae hardly anastomosed, a distinctive barrage line formed, and green fluorescent protein (GFP)-labeled hyphae quickly lost their fluorescence when encountering incompatible hyphae. In this study, transmission of a hypovirulence-conferring mycovirus to strains with different genetic backgrounds was attempted. Various chemical reagents considered to affect the programmed cell death pathway or cell wall modification were examined. Treatment with zinc compounds was shown to aid in transmission of mycoviruses to strains with different genetic backgrounds. In incompatible pairings, treatment with zinc compounds accelerated hyphal anastomosis; moreover, cytosolic GFP was transmitted to the newly joined hyphae. These results suggest that zinc compounds not only increase hyphal anastomosis but also attenuate heterogenic incompatibility.
authors.affiliation: Stress Cytology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan. ikeken@phoenix.kobe-u.ac.jp
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Kanako
authors.initials: K
authors.lastname: Inoue
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kida
authors.forename: Takahiro
authors.initials: T
authors.lastname: Uwamori
authors.forename: Atsuko
authors.initials: A
authors.lastname: Sasaki
authors.forename: Satoko
authors.initials: S
authors.lastname: Kanematsu
authors.forename: Pyoyun
authors.initials: P
authors.lastname: Park
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018014:Gene Transfer Techniques; D025301:Hyphae / Q000187:drug effects / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D012328:RNA Viruses / Q000302:isolation & purification / Q000502:physiology*; D053586:Virus Internalization / Q000187:drug effects*; D013098:Xylariales / Q000648:ultrastructure / Q000821:virology*; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D017967:Zinc Compounds
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