title: Multiple single-cell genomes provide insight into functions of uncultured Deltaproteobacteria in the human oral cavity.
abstract: Despite a long history of investigation, many bacteria associated with the human oral cavity have yet to be cultured. Studies that correlate the presence or abundance of uncultured species with oral health or disease highlight the importance of these community members. Thus, we sequenced several single-cell genomic amplicons from Desulfobulbus and Desulfovibrio (class Deltaproteobacteria) to better understand their function within the human oral community and their association with periodontitis, as well as other systemic diseases. Genomic data from oral Desulfobulbus and Desulfovibrio species were compared to other available deltaproteobacterial genomes, including from a subset of host-associated species. While both groups share a large number of genes with other environmental Deltaproteobacteria genomes, they encode a wide array of unique genes that appear to function in survival in a host environment. Many of these genes are similar to virulence and host adaptation factors of known human pathogens, suggesting that the oral Deltaproteobacteria have the potential to play a role in the etiology of periodontal disease.
authors.affiliation: Genome Science and Technology Program, University of Tennessee, Knoxville, TN, USA.
authors.forename: Alisha G
authors.initials: AG
authors.lastname: Campbell
authors.forename: James H
authors.initials: JH
authors.lastname: Campbell
authors.forename: Patrick
authors.initials: P
authors.lastname: Schwientek
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.forename: Alexander
authors.initials: A
authors.lastname: Sczyrba
authors.forename: Steve
authors.initials: S
authors.lastname: Allman
authors.forename: Clifford J
authors.initials: CJ
authors.lastname: Beall
authors.forename: Ann
authors.initials: A
authors.lastname: Griffen
authors.forename: Eugene
authors.initials: E
authors.lastname: Leys
authors.forename: Mircea
authors.initials: M
authors.lastname: Podar
mesh_terms: D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics*; D020564:Deltaproteobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020459:Genes, rRNA; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009055:Mouth / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000145:classification / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors
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