title: A new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim.
abstract: Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants' viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim.
authors.affiliation: Unidad de Bioquimica y Biologia Molecular de Plantas, Centro de Investigacion Cientifica de Yucatan AC, Calle 43 No. 130, Chuburna de Hidalgo, C.P. 97200 Merida, Yucatan, Mexico. hernan_villa66@hotmail.com
authors.forename: Hernan J
authors.initials: HJ
authors.lastname: Villanueva-Alonzo
authors.forename: Rosa Y
authors.initials: RY
authors.lastname: Us-Camas
authors.forename: Luisa A
authors.initials: LA
authors.lastname: López-Ochoa
authors.forename: Dominique
authors.initials: D
authors.lastname: Robertson
authors.forename: Orlene
authors.initials: O
authors.lastname: Guerra-Peraza
authors.forename: Yereni
authors.initials: Y
authors.lastname: Minero-García
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Moreno-Valenzuela
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D060467:Disease Resistance / Q000235:genetics; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D009029:Mosaic Viruses / Q000235:genetics*; D010935:Plant Diseases; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology
chemical_list: D010940:Plant Proteins