title: TRESK potassium channel in human T lymphoblasts.
abstract: TRESK (TWIK-related spinal cord K(+)) channel, encoded by KCNK18 gene, belongs to the double-pore domain K(+) channel family and in normal conditions is expressed predominantly in the central nervous system. In our previous patch-clamp study on Jurkat T lymphoblasts we have characterized highly selective K(+) channel with pharmacological profile identical to TRESK. In the present work, the presence of KCNK18 mRNA was confirmed in T lymphoblastic cell lines (Jurkat, JCaM, H9) but not in resting peripheral blood lymphocytes of healthy donors. Positive immunostaining for TRESK was demonstrated in lymphoblastic cell lines, in germinal centers of non-tumoral lymph nodes, and in clinical samples of T acute lymphoblastic leukemias/lymphomas. Besides detection in the plasma membrane, intracellular TRESK localization was also revealed. Possible involvement of TRESK channel in lymphocyte proliferation and tumorigenesis is discussed.
authors.affiliation: Center for Biomedical Research, University of Colima, Av. 25 de Julio 965, Villa San Sebastian, C.P. 28045 Colima, Mexico. Electronic address: amurusk@hotmail.com.
authors.forename: Dénison Selene
authors.initials: DS
authors.lastname: Sánchez-Miguel
authors.forename: Fernando
authors.initials: F
authors.lastname: García-Dolores
authors.forename: María
authors.initials: M
authors.lastname: Rosa Flores-Márquez
authors.forename: Iván
authors.initials: I
authors.lastname: Delgado-Enciso
authors.forename: Igor
authors.initials: I
authors.lastname: Pottosin
authors.forename: Oxana
authors.initials: O
authors.lastname: Dobrovinskaya
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology; D002471:Cell Transformation, Neoplastic; D003593:Cytoplasm / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C476574:KCNK18 protein, human; D009363:Neoplasm Proteins; D015221:Potassium Channels; D012333:RNA, Messenger