title: Structural characterization of coagulant Moringa oleifera Lectin and its effect on hemostatic parameters.
abstract: Lectins are carbohydrate recognition proteins. cMoL, a coagulant Moringa oleifera Lectin, was isolated from seeds of the plant. Structural studies revealed a heat-stable and pH resistant protein with 101 amino acids, 11.67 theoretical pI and 81% similarity with a M. oleifera flocculent protein. Secondary structure content was estimated as 46% α-helix, 12% β-sheets, 17% β-turns and 25% unordered structures belonging to the α/β tertiary structure class. cMoL significantly prolonged the time required for blood coagulation, activated partial thromboplastin (aPTT) and prothrombin times (PT), but was not so effective in prolonging aPTT in asialofetuin presence. cMoL acted as an anticoagulant protein on in vitro blood coagulation parameters and at least on aPTT, the lectin interacted through the carbohydrate recognition domain.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brazil. luciana_luz16@yahoo.com.br
authors.forename: Luciana de Andrade
authors.initials: Lde A
authors.lastname: Luz
authors.forename: Mariana Cristina Cabral
authors.initials: MC
authors.lastname: Silva
authors.forename: Rodrigo da Silva
authors.initials: Rda S
authors.lastname: Ferreira
authors.forename: Lucimeire Aparecida
authors.initials: LA
authors.lastname: Santana
authors.forename: Rosemeire Aparecida
authors.initials: RA
authors.lastname: Silva-Lucca
authors.forename: Reinhard
authors.initials: R
authors.lastname: Mentele
authors.forename: Maria Luiza Vilela
authors.initials: ML
authors.lastname: Oliva
authors.forename: Patricia Maria Guedes
authors.initials: PM
authors.lastname: Paiva
authors.forename: Luana Cassandra Breitenbach Barroso
authors.initials: LC
authors.lastname: Coelho
mesh_terms: D000595:Amino Acid Sequence; D003029:Coagulants / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D029588:Moringa oleifera / Q000737:chemistry*; D010314:Partial Thromboplastin Time; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D037121:Plant Lectins / Q000737:chemistry* / Q000494:pharmacology; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011517:Prothrombin Time
chemical_list: D003029:Coagulants; D010936:Plant Extracts; D037121:Plant Lectins