title: Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. "Mottenohoka" Is Enhanced by Suppressing Phosphorylation of p38MAPK.
abstract: Components from Dendranthema × grandiflorum cv. "Mottenohoka" that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth.
authors.affiliation: Yonezawa Women's Junior College, 6-15-1 Tohrimachi, Yonezawa, Yamagata 992-0025, Japan.
authors.forename: Atsuyoshi
authors.initials: A
authors.lastname: Nishina
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsukagoshi
authors.forename: Kunihisa
authors.initials: K
authors.lastname: Kozawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Koketsu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ninomiya
authors.forename: Shoei
authors.initials: S
authors.lastname: Furukawa
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