title: Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak.
abstract: A multiplex PCR was designed to detect Escherichia coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested, and 20.6% were positive for serogroup O104. The O104 isolates did not carry genes characteristic of the virulent hybrid strain.
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS, USA.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Paddock
authors.forename: J
authors.initials: J
authors.lastname: Bai
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.forename: D G
authors.initials: DG
authors.lastname: Renter
authors.forename: T G
authors.initials: TG
authors.lastname: Nagaraja
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D006824:Hybridization, Genetic; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers
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