title: Pharmacophore modeling, homology modeling, and in silico screening reveal mammalian target of rapamycin inhibitory activities for sotalol, glyburide, metipranolol, sulfamethizole, glipizide, and pioglitazone.
abstract: Mammalian target of rapamycin (mTOR) is a serine/threonine kinase and member of the PI3K-related kinase (PIKK) family. It plays a central role in integrating signals from metabolism, energy homeostasis, cell cycle, and stress response. Aberrant PI3K/mTOR activation is commonly observed in diseases such as cancer, diabetes and Alzheimer's disease. Accordingly, we developed common feature binding hypotheses for a set of 6 potent mTOR antagonists. The generated models were validated using receiver operating characteristic (ROC) curve analyses. To gain better insight into ligand-mTOR interactions, a homology model for the kinase domain of mTOR was built using the crystallographic structure of PI3Kγ as template. The optimal pharmacophore model was further improved based on detailed docking studies of potent training compound in the homology model. The modified binding model was employed as 3D search query to screen our in-house-built database of established drugs. Subsequent in vitro screening of captured hits showed that six of them have submicromolar to low micromolar bioactivities, namely, glyburide, metipranolol, sulfamethizole, glipizide, pioglitazone, and sotalol.
authors.affiliation: Drug Discovery Unit, Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman 11942, Jordan.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Khanfar
authors.forename: Majed M
authors.initials: MM
authors.lastname: AbuKhader
authors.forename: Saja
authors.initials: S
authors.lastname: Alqtaishat
authors.forename: Mutasem O
authors.initials: MO
authors.lastname: Taha
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D005913:Glipizide / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D014290:Metipranolol / Q000494:pharmacology; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D019869:Phosphatidylinositol 3-Kinases / Q000737:chemistry*; D000077205:Pioglitazone; D021281:Quantitative Structure-Activity Relationship; D012372:ROC Curve; D016415:Sequence Alignment; D013015:Sotalol / Q000494:pharmacology; D013419:Sulfamethizole / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors*; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D000319:Adrenergic beta-Antagonists; D000890:Anti-Infective Agents; D005913:Glipizide; D005905:Glyburide; D007004:Hypoglycemic Agents; D014290:Metipranolol; D019869:Phosphatidylinositol 3-Kinases; D000077205:Pioglitazone; D013015:Sotalol; D013419:Sulfamethizole; D058570:TOR Serine-Threonine Kinases; D045162:Thiazolidinediones; C546842:MTOR protein, human