title: Light regulation on growth, development, and secondary metabolism of marine-derived filamentous fungi.
abstract: Effects of different light conditions on development, growth, and secondary metabolism of three marine-derived filamentous fungi were investigated. Darkness irritated sexual development of Aspergillus glaucus HB1-19, while white, red, and blue lights improved its asexual behavior. The red and blue lights improved asexual stroma formation of Xylaria sp. (no. 2508), but the darkness and white light inhibited it. Differently, development of Halorosellinia sp. (no. 1403) turned out to be insensitive to any tested light irradiation. Upon the experimental data, no regularity was observed linking development with secondary metabolism. However, fungal growth showed inversely correlation with productions of major bioactive compounds (aspergiolide A, 1403C, and xyloketal B) from various strains. The results indicated that aspergiolide A biosynthesis favored blue light illumination, while 1403C and xyloketal B preferred red light irradiation. With the favorite light sensing conditions, productions of aspergiolide A, 1403C, and xyloketal B were enhanced by 32.9, 21.9, and 30.8 % compared with those in the dark, respectively. The phylogenetic analysis comparing the light-responding proteins of A. glaucus HB 1-19 with those in other systems indicated that A. glaucus HB 1-19 was closely related to Aspergillus spp. especially A. nidulans in spite of its role of marine-derived fungus. It indicated that marine fungi might conserve its light response system when adapting the marine environment. This work also offers useful information for process optimization involving light regulation on growth and metabolism for drug candidate production from light-sensitive marine fungi.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
authors.forename: Menghao
authors.initials: M
authors.lastname: Cai
authors.forename: Zhe
authors.initials: Z
authors.lastname: Fang
authors.forename: Chuanpeng
authors.initials: C
authors.lastname: Niu
authors.forename: Xiangshan
authors.initials: X
authors.lastname: Zhou
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D059001:Aquatic Organisms / Q000145:classification / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D001688:Biological Products / Q000378:metabolism; D016000:Cluster Analysis; D003624:Darkness; D005658:Fungi / Q000145:classification / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D008027:Light; D010802:Phylogeny; D011714:Pyrans / Q000378:metabolism; D064210:Secondary Metabolism / Q000528:radiation effects*
chemical_list: D000880:Anthraquinones; D001688:Biological Products; D011714:Pyrans; C569938:SZ 685C; C542894:aspergiolide A; C515343:xyloketal B
references.citation: Fungal Genet Biol. 2010 Dec;47(12):962-72
references.pmid: 20816830
references.citation: Curr Opin Microbiol. 2006 Dec;9(6):566-71
references.pmid: 17067849
references.citation: Appl Microbiol Biotechnol. 2010 Feb;85(5):1259-77
references.pmid: 19915832
references.citation: Fungal Genet Biol. 2011 Jan;48(1):15-22
references.pmid: 20433937
references.citation: Science. 1997 May 2;276(5313):763-9
references.pmid: 9115195
references.citation: Biochem Pharmacol. 2009 Oct 15;78(8):941-50
references.pmid: 19481065
references.citation: Bioresour Technol. 2011 Nov;102(22):10750-3
references.pmid: 21955880
references.citation: Brief Bioinform. 2004 Jun;5(2):150-63
references.pmid: 15260895
references.citation: EMBO J. 2003 Sep 15;22(18):4846-55
references.pmid: 12970196
references.citation: Curr Biol. 2008 Feb 26;18(4):255-9
references.pmid: 18291652
references.citation: J Ind Microbiol Biotechnol. 2006 May;33(5):325-37
references.pmid: 16429315
references.citation: Curr Biol. 2005 Oct 25;15(20):1833-8
references.pmid: 16243030
references.citation: Genetics. 1998 Aug;149(4):1777-86
references.pmid: 9691036
references.citation: Mol Biol Cell. 2008 Aug;19(8):3254-62
references.pmid: 18495868
references.citation: Mol Genet Genomics. 2009 Jan;281(1):35-42
references.pmid: 18936976
references.citation: Science. 2008 Jun 13;320(5882):1504-6
references.pmid: 18556559
references.citation: Microbiol Mol Biol Rev. 2002 Sep;66(3):447-59, table of contents
references.pmid: 12208999
references.citation: Immunopharmacol Immunotoxicol. 2010 Jun;32(2):228-37
references.pmid: 20441539
references.citation: Fungal Genet Biol. 2010 Nov;47(11):900-8
references.pmid: 20573560
references.citation: Arch Microbiol. 2007 Jul;188(1):69-79
references.pmid: 17375284
references.citation: Mol Microbiol. 2007 Apr;64(1):5-15
references.pmid: 17376067
references.citation: Bioresour Technol. 2010 Aug;101(15):6059-68
references.pmid: 20359887
references.citation: Science. 2002 Aug 2;297(5582):777-8
references.pmid: 12161636
references.citation: Biotechnol Adv. 2004 Jan;22(3):189-259
references.pmid: 14665401
references.citation: Br J Pharmacol. 2010 Feb 1;159(3):689-97
references.pmid: 20128807
references.citation: J Biol Rhythms. 2003 Jun;18(3):195-205
references.pmid: 12828277
references.citation: Genes Dev. 1990 Sep;4(9):1473-82
references.pmid: 2253875
references.citation: J Ind Microbiol Biotechnol. 2005 Mar;32(3):103-8
references.pmid: 15750807
references.citation: Magn Reson Chem. 2007 Nov;45(11):1006-9
references.pmid: 17894425