title: Probing mercury(II)-DNA interactions by nanopore stochastic sensing.
abstract: In this work, DNA-Hg(II) interactions were investigated by monitoring the translocation of DNA hairpins in a protein ion channel in the absence and presence of metal ions. Our experiments demonstrate that target-specific hairpin structures could be stabilized much more significantly by mercuric ions than by the stem length and the loop size of the hairpin due to the formation of Thymine-Hg(II)-Thymine complexes. In addition, the designed DNA probe allows the development of a highly sensitive nanopore sensor for Hg(2+) with a detection limit of 25 nM. Further, the sensor is specific, and other tested metal ions including Pb(2+), Cu(2+), Cd(2+), and so on with concentrations of up to 2 orders of magnitude greater than that of Hg(2+) would not interfere with the mercury detection.
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, 3101 South Dearborn Street, Chicago, Illinois 60616, United States.
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Qitao
authors.initials: Q
authors.lastname: Zhao
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Kang
authors.forename: Xiyun
authors.initials: X
authors.lastname: Guan
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D055664:Electrochemical Techniques; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055029:Inverted Repeat Sequences; D007477:Ions / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008628:Mercury / Q000032:analysis*; D058608:Nanopores; D013941:Thymine / Q000737:chemistry
chemical_list: D015342:DNA Probes; D006460:Hemolysin Proteins; D007477:Ions; D008051:Lipid Bilayers; D008628:Mercury; D013941:Thymine
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