title: Fatty acid profiling of the seed oils of some varieties of field peas (Pisum sativum) by RP-LC/ESI-MS/MS: towards the development of an oilseed pea.
abstract: Reversed-phase liquid chromatography coupled to negative-ion electrospray tandem mass spectrometry (RP-LC/ESI-MS/MS) was used to study the fatty acid profile from the oil of harvested field pea (Pisum sativum) varieties as part of a research project to develop this legume as a commercial oilseed for Canada. The seed oils from pea samples contained palmitic and stearic acids as major saturated fatty acids. Oleic, linoleic and linolenic acids were the major unsaturated fatty acids found. Small percentages of other long chain fatty acids were also detected. This profile suggests that the species of field pea investigated might have the potential to be used as raw materials to develop a future new oilseed crop for the food industry. Fatty acid extracts did not require further manipulation before final analysis by RP-LC/ESI-MS/MS, indicating the utility and relative simplicity of this technique for future screening studies.
authors.affiliation: Bioresource Engineering Department, McGill University, Macdonald Campus, 21,111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, Canada H9X 3V9. manuel.villalobossolis@mail.mcgill.ca
authors.forename: Manuel Ivan
authors.initials: MI
authors.lastname: Villalobos Solis
authors.forename: Anil
authors.initials: A
authors.lastname: Patel
authors.forename: Valérie
authors.initials: V
authors.lastname: Orsat
authors.forename: Jaswinder
authors.initials: J
authors.lastname: Singh
authors.forename: Mark
authors.initials: M
authors.lastname: Lefsrud
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry*; D018532:Pisum sativum / Q000737:chemistry* / Q000145:classification; D010938:Plant Oils / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005227:Fatty Acids; D010938:Plant Oils