title: Structures of biomolecular ions in the gas phase probed by infrared light sources.
abstract: Infrared (IR) spectroscopy of biomolecular ions combines mass spectrometry's high sensitivity and ability to analyze complex mixtures with the enhanced structural information available from vibrational spectroscopy. IR spectroscopy is in principle well placed to distinguish isomers and allow chemical classification of unknown molecules. This review gives an outline of current instrumentation, spectroscopic approaches, and potential bottlenecks. We discuss the most promising applications in bioanalytical mass spectrometry in view of recent experimental results, as well as future applications based on bioinformatics.
authors.affiliation: Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, USA. polfer@chem.ufl.edu
authors.forename: Corey N
authors.initials: CN
authors.lastname: Stedwell
authors.forename: Johan F
authors.initials: JF
authors.lastname: Galindo
authors.forename: Adrian E
authors.initials: AE
authors.lastname: Roitberg
authors.forename: Nicolas C
authors.initials: NC
authors.lastname: Polfer
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D005740:Gases / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D005740:Gases; D007477:Ions; D010455:Peptides