title: SH3 interactome conserves general function over specific form.
abstract: Src homology 3 (SH3) domains bind peptides to mediate protein-protein interactions that assemble and regulate dynamic biological processes. We surveyed the repertoire of SH3 binding specificity using peptide phage display in a metazoan, the worm Caenorhabditis elegans, and discovered that it structurally mirrors that of the budding yeast Saccharomyces cerevisiae. We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast. We found that the worm SH3 interactome resembles the analogous yeast network because it is significantly enriched for proteins with roles in endocytosis. Nevertheless, orthologous SH3 domain-mediated interactions are highly rewired. Our results suggest a model of network evolution where general function of the SH3 domain network is conserved over its specific form.
authors.affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Xin
authors.forename: David
authors.initials: D
authors.lastname: Gfeller
authors.forename: Jackie
authors.initials: J
authors.lastname: Cheng
authors.forename: Raffi
authors.initials: R
authors.lastname: Tonikian
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Ailan
authors.initials: A
authors.lastname: Guo
authors.forename: Lianet
authors.initials: L
authors.lastname: Lopez
authors.forename: Alevtina
authors.initials: A
authors.lastname: Pavlenco
authors.forename: Adenrele
authors.initials: A
authors.lastname: Akintobi
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Rual
authors.forename: Bridget
authors.initials: B
authors.lastname: Currell
authors.forename: Somasekar
authors.initials: S
authors.lastname: Seshagiri
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.forename: Xinping
authors.initials: X
authors.lastname: Yang
authors.forename: Yun A
authors.initials: YA
authors.lastname: Shen
authors.forename: Kourosh
authors.initials: K
authors.lastname: Salehi-Ashtiani
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Cheng
authors.forename: Dryden
authors.initials: D
authors.lastname: Bouamalay
authors.forename: Adrien
authors.initials: A
authors.lastname: Lugari
authors.forename: David E
authors.initials: DE
authors.lastname: Hill
authors.forename: Mark L
authors.initials: ML
authors.lastname: Grimes
authors.forename: David G
authors.initials: DG
authors.lastname: Drubin
authors.forename: Barth D
authors.initials: BD
authors.lastname: Grant
authors.forename: Marc
authors.initials: M
authors.lastname: Vidal
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: Sachdev S
authors.initials: SS
authors.lastname: Sidhu
authors.forename: Gary D
authors.initials: GD
authors.lastname: Bader
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D004705:Endocytosis / Q000235:genetics; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D040681:Structural Homology, Protein; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains / Q000235:genetics*
chemical_list: D029742:Caenorhabditis elegans Proteins; D029701:Saccharomyces cerevisiae Proteins
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