title: Minimal biofilm eradication concentration of antimicrobial agents against nontypeable Haemophilus influenzae isolated from middle ear fluids of intractable acute otitis media.
abstract: Nontypeable Haemophilus influenzae (NTHi) makes the clinical course of acute otitis media (AOM) intractable by forming a biofilm that may hamper the clearance of the bacteria from middle ear cavity. In this study, we evaluated the minimum biofilm eradication concentration (MBEC) of antimicrobial agents against biofilm-forming NTHi strains. Twelve NTHi strains isolated from middle ear fluids of Japanese children with intractable AOM before antimicrobial treatment were evaluated for MBEC of fluoroquinolones in comparison with those of β-lactams and macrolides. AMPC and CDTR required much higher concentration, i.e., high MBECs, to suppress the biofilm formation of NTHi. In contrast, fluoroquinolones followed by macrolides showed lower MBECs. MBEC would be a good parameter to infer the efficacies of antimicrobials against NTHi in biofilm.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, 811-1, Kimiidera, Wakayama, Wakayama, 640-8500, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Takei
authors.forename: Muneki
authors.initials: M
authors.lastname: Hotomi
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamanaka
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D024841:Fluoroquinolones / Q000494:pharmacology; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D018942:Macrolides / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010033:Otitis Media / Q000382:microbiology*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D018942:Macrolides; D047090:beta-Lactams