title: Separation science: Principles and applications for the analysis of bionanoparticles by asymmetrical flow field-flow fractionation (AF4).
abstract: Field-flow fractionation is an analytical technique that allows the separation of particles over a size range, from a few nanometers to several microns in diameter. The separation takes place under mild conditions and is suited for the analysis of neutral or charged particles. A single measurement yields the size and concentration of each component of a mixture. However, developing a suitable fractionation method can be tedious and time-consuming. In this chapter, we present asymmetrical flow field-flow fractionation (AF4) conditions that have proven their reliability for the analysis of quantum dots and other nanoparticles in the 5-50 nm size range. Common pitfalls are emphasized together with strategies to overcome them.
authors.affiliation: Faculty of Pharmacy, and Department of Pharmacology & Therapeutics, Faculty of Medicine, Université de Montréal and McGill University, Montreal, QC, Canada.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Moquin
authors.forename: Françoise M
authors.initials: FM
authors.lastname: Winnik
authors.forename: Dusica
authors.initials: D
authors.lastname: Maysinger
mesh_terms: D031521:Fractionation, Field Flow / Q000379:methods*; D053758:Nanoparticles