title: Host susceptibility to periodontitis: mapping murine genomic regions.
abstract: Host susceptibility to periodontal infection is controlled by genetic factors. As a step toward identifying and cloning these factors, we generated an A/J x BALB/cJ F2 mouse resource population. A genome-wide search for Quantitative Trait Loci (QTL) associated with periodontitis was performed. We aimed to quantify the phenotypic response of the progenies to periodontitis by microCT analysis, to perform a genome-wide search for QTL associated with periodontitis, and, finally, to suggest candidate genes for periodontitis. We were able to produce 408 F2 mice. All mice were co-infected with Porphyromonas gingivalis and Fusobacterium nucleatum bacteria. Six weeks following infection, alveolar bone loss was quantified by computerized tomography (microCT) technology. We found normal distribution of the phenotype, with 2 highly significant QTL on chromosomes 5 and 3. A third significant QTL was found on chromosome 1. Candidate genes were suggested, such as Toll-like receptors (TLR) 1 and 6, chemokines, and bone-remodeling genes (enamelin, ameloblastin, and amelotin). This report shows that periodontitis in mice is a polygenic trait with highly significant mapped QTL.
authors.affiliation: Department of Prosthodontics, Hadassah Medical Center, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Shusterman
authors.forename: C
authors.initials: C
authors.lastname: Durrant
authors.forename: R
authors.initials: R
authors.lastname: Mott
authors.forename: D
authors.initials: D
authors.lastname: Polak
authors.forename: A
authors.initials: A
authors.lastname: Schaefer
authors.forename: E I
authors.initials: EI
authors.lastname: Weiss
authors.forename: F A
authors.initials: FA
authors.lastname: Iraqi
authors.forename: Y
authors.initials: Y
authors.lastname: Houri-Haddad
mesh_terms: D016301:Alveolar Bone Loss / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D000818:Animals; D002874:Chromosome Mapping; D060085:Coinfection; D016967:Fusobacterium nucleatum / Q000502:physiology; D020022:Genetic Predisposition to Disease; D054884:Host-Pathogen Interactions / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D020412:Multifactorial Inheritance; D010518:Periodontitis / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000502:physiology; D040641:Quantitative Trait Loci / Q000235:genetics*