title: Transgenic manipulation of plant embryo sacs tracked through cell-type-specific fluorescent markers: cell labeling, cell ablation, and adventitious embryos.
abstract: Expression datasets relating to the Arabidopsis female gametophyte have enabled the creation of a tool set which allows simultaneous visual tracking of each specific cell type (egg, synergids, central cell, and antipodals). This cell-specific, fluorescent labeling tool-set functions from gametophyte cellularization through fertilization and early embryo development. Using this system, cell fates were tracked within Arabidopsis ovules following molecular manipulations, such as the ablation of the egg and/or synergids. Upon egg cell ablation, it was observed that a synergid can switch its developmental fate to become egg/embryo-like upon loss of the native egg. Also, manipulated was the fate of the somatic ovular cells, which can become egg- and embryo-like, reminiscent of adventitious embryony. These advances represent initial steps toward engineering synthetic apomixis resulting in seed derived wholly from the maternal plant. The end goal of applied apomixis research, fixing important agronomic traits such as hybrid vigor, would be a key benefit to agricultural productivity.
authors.affiliation: Agricultural Biotechnology, DuPont Pioneer, Johnston, IA 50131-1004, USA. shai.lawit@pioneer.com
authors.forename: Shai J
authors.initials: SJ
authors.lastname: Lawit
authors.forename: Mark A
authors.initials: MA
authors.lastname: Chamberlin
authors.forename: April
authors.initials: A
authors.lastname: Agee
authors.forename: Eric S
authors.initials: ES
authors.lastname: Caswell
authors.forename: Marc C
authors.initials: MC
authors.lastname: Albertsen
mesh_terms: D060832:Apomixis / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D005306:Fertilization; D005453:Fluorescence; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D009154:Mutation; D009928:Organ Specificity; D056771:Ovule / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D010641:Phenotype; D012639:Seeds / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D029681:Arabidopsis Proteins; D005819:Genetic Markers
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