title: Virulence gene typing of methicillin-resistant Staphylococcus aureus as a complement in epidemiological typing.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) has widely spread to all parts of the world. For surveillance and effective infection control molecular typing is required. We have evaluated the utility of virulence gene determination as a complementary tool for epidemiological typing of MRSA in relation to spa-typing and pulsed-field gel electrophoresis (PFGE). We assessed 63 community-acquired MRSA (CA-MRSA) isolates detected in the West part of Sweden for 30 virulence factor genes (VF) and agr allele variations by serial polymerase chain reaction (PCR) assays. These isolates belonged to sequence types (ST) 8, 80, 45 and 30 as classified by multilocus sequence typing. The isolates in each spa-type and PFGE-type were examined over an extended time-period and constituted a varying number of PFGE-subtypes (5-14) and spa-types (3-11) within four major PFGE types. Each ST had a unique VF profile. For isolates within a major PFGE type showing high diversity both in PFGE subtypes and spa the VF profile varied as well in contrast to those with low diversity where no alterations were seen. Thus, the accuracy of each typing method does not only vary by the method per se but is rather dependent on the genetic repertoire of the typed strains and genes evaluated. For strains demonstrating high diversity VF typing may be a useful complement in the epidemiological investigations, and may highlight the accurate discriminatory power of spa or PFGE typing.
authors.affiliation: Institution for Biomedicine, Department of Infectious Disease, University of Gothenburg, Gothenburg, Sweden. forough.nowrouzian@microbio.gu.se
authors.forename: Forough L
authors.initials: FL
authors.lastname: Nowrouzian
authors.forename: Nahid
authors.initials: N
authors.lastname: Karami
authors.forename: Christina
authors.initials: C
authors.lastname: Welinder-Olsson
authors.forename: Christina
authors.initials: C
authors.lastname: Ahrén
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013548:Sweden / Q000453:epidemiology; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors