title: Interlocking of β-carotene in beta-lactoglobulin aggregates produced under high pressure.
abstract: Vitamin A deficiency is one of the major causes of mortality and morbidity in the developing World. This deficiency can be prevented by alimentary or pharmaceutical supplementation. However, both vitamin A oxidation and isomerization should be prevented, as these phenomenons result in loss of nutritional efficacy. The aim of this study was to investigate the effect of a food protein matrix, β-lactoglobulin (β-Lg) aggregates produced by high pressure (HP), on the stabilization of β-carotene during storage and gastro-duodenal digestion and therefore on its bioavailability. In vitro gastro-duodenal digestion of β-Lg aggregates entrapping β-carotene showed that up to 12% and 33% of total β-carotene was released after peptic and pancreatic digestion, respectively. Overall, our study showed that β-Lg aggregates are efficient for caging and stabilization of β-carotene during storage and digestion. Hence, it may be an interesting approach for the protection and the delivery of vitamin A.
authors.affiliation: UR 1268, INRA Biopolymères Interactions Assemblages-équipe Fonctions et Interactions des Protéines, rue de la Géraudière, B.P. 71627, 44316 Nantes Cedex 03, France.
authors.forename: Azza
authors.initials: A
authors.lastname: Mensi
authors.forename: Yvan
authors.initials: Y
authors.lastname: Choiset
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Reboul
authors.forename: Patrick
authors.initials: P
authors.lastname: Borel
authors.forename: Claire
authors.initials: C
authors.lastname: Guyon
authors.forename: Marie
authors.initials: M
authors.lastname: de Lamballerie
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
mesh_terms: D001682:Biological Availability; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis; D004063:Digestion; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry*; D008954:Models, Biological; D011312:Pressure; D014802:Vitamin A Deficiency / Q000178:diet therapy / Q000378:metabolism; D019207:beta Carotene / Q000737:chemistry* / Q000493:pharmacokinetics
chemical_list: D004337:Drug Carriers; D007782:Lactoglobulins; D019207:beta Carotene