title: Structural glycomic analyses at high sensitivity: a decade of progress.
abstract: The field of glycomics has recently advanced in response to the urgent need for structural characterization and quantification of complex carbohydrates in biologically and medically important applications. The recent success of analytical glycobiology at high sensitivity reflects numerous advances in biomolecular mass spectrometry and its instrumentation, capillary and microchip separation techniques, and microchemical manipulations of carbohydrate reactivity. The multimethodological approach appears to be necessary to gain an in-depth understanding of very complex glycomes in different biological systems.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Alley
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D005260:Female; D054794:Glycomics / Q000379:methods; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D008745:Methylation; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002241:Carbohydrates; D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D019158:N-Acetylneuraminic Acid
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 5;793(1):167-79
references.pmid: 12880864
references.citation: Glycobiology. 2007 Dec;17(12):1344-56
references.pmid: 17884841
references.citation: J Am Soc Mass Spectrom. 2009 Apr;20(4):539-56
references.pmid: 19181540
references.citation: J Proteome Res. 2010 Aug 6;9(8):4138-51
references.pmid: 20578730
references.citation: Anal Chem. 1999 Aug 1;71(15):3206-18
references.pmid: 10450162
references.citation: J Am Chem Soc. 2003 Dec 31;125(52):16213-9
references.pmid: 14692762
references.citation: Anal Chem. 2010 Dec 15;82(24):10095-101
references.pmid: 21077632
references.citation: Mol Biosyst. 2009 Nov;5(11):1298-302
references.pmid: 19823744
references.citation: Anal Chem. 1998 Jul 15;70(14):3053-9
references.pmid: 9684552
references.citation: Anal Chem. 2009 Dec 1;81(23):9546-52
references.pmid: 19874002
references.citation: Anal Chem. 2010 May 1;82(9):3719-26
references.pmid: 20387804
references.citation: Glycobiology. 2012 Dec;22(12):1684-708
references.pmid: 22781126
references.citation: Anal Chem. 2011 Jul 1;83(13):5329-36
references.pmid: 21591710
references.citation: Electrophoresis. 2011 Dec;32(24):3516-25
references.pmid: 22120947
references.citation: Anal Chem. 2011 Sep 1;83(17):6738-45
references.pmid: 21774516
references.citation: Rapid Commun Mass Spectrom. 2009 Jan;23(2):303-12
references.pmid: 19089860
references.citation: Glycobiology. 2010 Sep;20(9):1160-9
references.pmid: 20507882
references.citation: Anal Chem. 2004 Apr 15;76(8):2343-54
references.pmid: 15080747
references.citation: Anal Chem. 2007 Aug 15;79(16):6064-73
references.pmid: 17630715
references.citation: Glycobiology. 2012 Mar;22(3):389-99
references.pmid: 22038479
references.citation: Anal Chem. 2007 Sep 15;79(18):7170-5
references.pmid: 17685584
references.citation: J Proteome Res. 2008 Mar;7(3):1109-17
references.pmid: 18237112
references.citation: J Am Soc Mass Spectrom. 2006 Jun;17(6):844-854
references.pmid: 16603372
references.citation: J Proteome Res. 2012 Feb 3;11(2):576-85
references.pmid: 22148953
references.citation: Anal Chem. 2009 Nov 1;81(21):8818-25
references.pmid: 19807107
references.citation: Proteomics. 2009 Aug;9(15):3796-801
references.pmid: 19670245
references.citation: Rapid Commun Mass Spectrom. 2008;22(5):721-34
references.pmid: 18265433
references.citation: Mol Cell Proteomics. 2009 May;8(5):986-94
references.pmid: 19181623
references.citation: Anal Chem. 2010 Mar 15;82(6):2588-93
references.pmid: 20170179
references.citation: Anal Chem. 2008 Oct 1;80(19):7534-42
references.pmid: 18778038
references.citation: J Am Soc Mass Spectrom. 2010 Mar;21(3):348-57
references.pmid: 20022260
references.citation: Anal Chem. 2010 Sep 1;82(17):7408-15
references.pmid: 20684520
references.citation: Biochemistry. 1994 Nov 22;33(46):13524-30
references.pmid: 7947762
references.citation: Anal Biochem. 2009 Apr 15;387(2):162-70
references.pmid: 19454239
references.citation: Anal Chem. 2010 Apr 1;82(7):3023-31
references.pmid: 20230064
references.citation: Electrophoresis. 2011 Jan;32(2):246-53
references.pmid: 21254122
references.citation: Anal Chem. 2007 May 15;79(10):3830-42
references.pmid: 17397137
references.citation: J Biol Chem. 1968 Feb 10;243(3):616-26
references.pmid: 5637714
references.citation: Lab Chip. 2005 Mar;5(3):298-307
references.pmid: 15726206
references.citation: Anal Chem. 2006 Dec 15;78(24):8491-8
references.pmid: 17165844
references.citation: Rapid Commun Mass Spectrom. 1996;10(1):100-3
references.pmid: 8563011
references.citation: J Biochem. 1964 Feb;55:205-8
references.pmid: 14135466
references.citation: Proteomics. 2010 Jun;10(12):2330-6
references.pmid: 20391534
references.citation: Rapid Commun Mass Spectrom. 1996;10(9):1027-32
references.pmid: 8755235
references.citation: Nature. 1996 Apr 4;380(6573):461-2
references.pmid: 8602248
references.citation: Analyst. 2011 Sep 21;136(18):3663-71
references.pmid: 21776491
references.citation: Mol Cell Proteomics. 2011 Oct;10(10):M111.010090
references.pmid: 21653738
references.citation: Rapid Commun Mass Spectrom. 2002;16(12):1199-204
references.pmid: 12112272
references.citation: Mol Cell Proteomics. 2002 Jan;1(1):11-8
references.pmid: 12096136
references.citation: J Am Soc Mass Spectrom. 2005 May;16(5):622-30
references.pmid: 15862764
references.citation: Glycobiology. 2008 Dec;18(12):1105-18
references.pmid: 18818422
references.citation: J Mass Spectrom. 2005 May;40(5):642-53
references.pmid: 15751107
references.citation: J Am Soc Mass Spectrom. 2007 Oct;18(10):1799-812
references.pmid: 17719235
references.citation: Anal Chem. 1999 Apr 1;71(7):1431-40
references.pmid: 10204042
references.citation: Anal Chem. 2010 Dec 15;82(24):10021-9
references.pmid: 21077635
references.citation: Anal Chem. 1995 Nov 1;67(21):4028-31
references.pmid: 8633763
references.citation: Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2302-6
references.pmid: 1706520
references.citation: Anal Chem. 2005 Nov 1;77(21):6954-9
references.pmid: 16255595
references.citation: J Proteome Res. 2011 Feb 4;10(2):856-68
references.pmid: 21133381
references.citation: Eur J Biochem. 1991 Aug 1;199(3):647-52
references.pmid: 1868849
references.citation: Glycobiology. 2007 Jul;17(7):677-87
references.pmid: 17384119
references.citation: Electrophoresis. 1996 Feb;17(2):412-7
references.pmid: 8900952
references.citation: Glycobiology. 2009 Oct;19(10):1136-49
references.pmid: 19671626
references.citation: Electrophoresis. 2011 Jan;32(1):14-29
references.pmid: 21171110
references.citation: J Biol Chem. 1990 Nov 15;265(32):19611-23
references.pmid: 2246247
references.citation: Anal Chem. 1997 Jul 1;69(13):2504-9
references.pmid: 21639386
references.citation: J Proteome Res. 2012 Mar 2;11(3):1949-57
references.pmid: 22256963
references.citation: J Am Soc Mass Spectrom. 2005 May;16(5):631-46
references.pmid: 15862765
references.citation: Anal Chem. 2010 Aug 1;82(15):6636-42
references.pmid: 20590124
references.citation: Electrophoresis. 2011 May;32(10):1129-32
references.pmid: 21500212
references.citation: Anal Chem. 2007 Jul 1;79(13):5051-7
references.pmid: 17539604
references.citation: J Biol Chem. 2010 Aug 6;285(32):24882-91
references.pmid: 20507986
references.citation: Electrophoresis. 2011 Dec;32(24):3499-509
references.pmid: 22180204
references.citation: J Am Soc Mass Spectrom. 2008 Nov;19(11):1706-15
references.pmid: 18760624
references.citation: Electrophoresis. 1997 Jun;18(7):1136-41
references.pmid: 9237569
references.citation: Anal Chem. 1992 Jul 1;64(13):1328-36
references.pmid: 1503214
references.citation: Anal Biochem. 2011 Nov 1;418(1):1-9
references.pmid: 21803021
references.citation: Rapid Commun Mass Spectrom. 2005;19(24):3681-8
references.pmid: 16287041
references.citation: Anal Biochem. 1995 Sep 20;230(2):229-38
references.pmid: 7503412
references.citation: Nat Chem Biol. 2011 Feb;7(2):78-80
references.pmid: 21196935
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Oct 1;879(27):2928-34
references.pmid: 21907640
references.citation: Anal Chem. 2010 Oct 1;82(19):8300-6
references.pmid: 20831242
references.citation: Glycobiology. 2005 Apr;15(4):361-7
references.pmid: 15574802
references.citation: Anal Chem. 2008 Jul 1;80(13):5211-8
references.pmid: 18484736
references.citation: J Proteome Res. 2009 Aug;8(8):3816-23
references.pmid: 19449840
references.citation: Anal Chem. 2007 May 15;79(10):3894-900
references.pmid: 17411071
references.citation: J Proteome Res. 2011 Apr 1;10(4):1548-57
references.pmid: 21214271
references.citation: Rapid Commun Mass Spectrom. 2006;20(8):1381-9
references.pmid: 16557638
references.citation: Cell. 2010 May 28;141(5):897-907
references.pmid: 20510933
references.citation: J Proteome Res. 2010 Jun 4;9(6):3062-72
references.pmid: 20345175
references.citation: Anal Bioanal Chem. 2005 Mar;381(6):1130-7
references.pmid: 15761738
references.citation: J Am Soc Mass Spectrom. 2009 Sep;20(9):1660-71
references.pmid: 19546010
references.citation: Glycobiology. 2010 May;20(5):629-47
references.pmid: 20110246
references.citation: Chem Rev. 2002 Feb;102(2):321-69
references.pmid: 11841246
references.citation: Electrophoresis. 2010 Nov;31(22):3783-6
references.pmid: 20972992
references.citation: J Proteome Res. 2008 Jan;7(1):367-74
references.pmid: 18047270
references.citation: J Biol Chem. 2008 Nov 28;283(48):33674-84
references.pmid: 18818196
references.citation: Anal Chem. 2005 Jan 15;77(2):527-33
references.pmid: 15649049
references.citation: J Chromatogr. 1991 Oct 18;559(1-2):223-35
references.pmid: 1761624
references.citation: Anal Chem. 2010 Feb 15;82(4):1498-508
references.pmid: 20108906
references.citation: Rapid Commun Mass Spectrom. 2008 Apr;22(7):1047-52
references.pmid: 18327885
references.citation: J Chromatogr. 1990 Jan 19;499:177-96
references.pmid: 2324207
references.citation: J Am Soc Mass Spectrom. 2005 May;16(5):683-96
references.pmid: 15862770
references.citation: Electrophoresis. 2008 Jun;29(12):2485-507
references.pmid: 18512669
references.citation: J Biol Chem. 1984 Sep 10;259(17):10700-4
references.pmid: 6206060
references.citation: Methods Mol Biol. 1993;14:151-9
references.pmid: 8348231
references.citation: Immunology. 2009 Mar;126(3):336-45
references.pmid: 18710403
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Feb 1;878(3-4):403-8
references.pmid: 20036624
references.citation: Anal Chem. 2008 May 15;80(10):3838-45
references.pmid: 18426228
references.citation: Electrophoresis. 2005 May;26(10):2034-46
references.pmid: 15841499
references.citation: Electrophoresis. 2008 Nov;29(20):4203-14
references.pmid: 18925582
references.citation: Anal Chem. 2003 Oct 15;75(20):5628-37
references.pmid: 14710847
references.citation: Glycobiology. 2010 Aug;20(8):959-69
references.pmid: 20356825
references.citation: Anal Chem. 2001 Dec 15;73(24):6063-9
references.pmid: 11791581
references.citation: J Biol Chem. 2010 May 21;285(21):16012-22
references.pmid: 20233717
references.citation: Rapid Commun Mass Spectrom. 2004;18(20):2401-14
references.pmid: 15386629
references.citation: Cell. 2010 Nov 24;143(5):672-6
references.pmid: 21111227
references.citation: Mass Spectrom Rev. 2009 Mar-Apr;28(2):207-22
references.pmid: 18973241
references.citation: Anal Chem. 2005 Aug 1;77(15):4962-8
references.pmid: 16053310
references.citation: Science. 2012 Aug 24;337(6097):975-80
references.pmid: 22923583
references.citation: J Mass Spectrom. 2000 Oct;35(10):1178-90
references.pmid: 11110090
references.citation: Anal Chem. 2008 Sep 1;80(17):6809-14
references.pmid: 18671408
references.citation: J Agric Food Chem. 2011 Apr 27;59(8):4255-63
references.pmid: 21384928
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2011 May 15;879(17-18):1196-225
references.pmid: 21145794
references.citation: Proteomics. 2009 Apr;9(7):1939-51
references.pmid: 19288519
references.citation: Nat Rev Mol Cell Biol. 2012 Jun 22;13(7):448-62
references.pmid: 22722607
references.citation: Anal Chem. 2006 Sep 1;78(17):6081-7
references.pmid: 16944887
references.citation: Anal Chem. 2007 Jan 1;79(1):355-63
references.pmid: 17194161
references.citation: J Proteome Res. 2011 Apr 1;10(4):1755-64
references.pmid: 21214223
references.citation: Rapid Commun Mass Spectrom. 2005;19(23):3421-8
references.pmid: 16252310
references.citation: Glycobiology. 2008 May;18(5):353-66
references.pmid: 18256178
references.citation: Anal Chem. 2003 Sep 15;75(18):4895-903
references.pmid: 14674469
references.citation: J Am Soc Mass Spectrom. 2011 Aug;22(8):1309-17
references.pmid: 21953184
references.citation: Anal Chem. 2010 Mar 15;82(6):2421-5
references.pmid: 20178317
references.citation: Curr Opin Cell Biol. 1992 Dec;4(6):1017-23
references.pmid: 1485955
references.citation: J Am Soc Mass Spectrom. 2005 May;16(5):647-59
references.pmid: 15862766
references.citation: Rapid Commun Mass Spectrom. 2008 Sep;22(17):2723-30
references.pmid: 18677720
references.citation: J Am Soc Mass Spectrom. 2011 Mar;22(3):568-81
references.pmid: 21472575
references.citation: Anal Chem. 2007 Aug 1;79(15):5777-84
references.pmid: 17605469
references.citation: Anal Chem. 2010 Jun 15;82(12):5095-106
references.pmid: 20491449