title: Effect of Y-27632 on the cultured retinal neurocytes of rats.
abstract: OBJECTIVE
  To investigate the effect of Y-27632 on the survival and neurite outgrowth of the cultured retinal neurocytes.
  
  
  METHODS
  After the postnatal day 2-3, Sprague-Dawley retinal neurocytes were cultured for 48 hours, the culture media was replaced with serum-free media (control group) and serum-free media contained 30µmol/L Y-27632 (Y-27632 group), and the cells were continually cultured another 48 hours. The cultured retinal neurocytes were identified with anti-neuron specific enolase (NSE) immunocytochemistry. The survival state of those cells was estimated by MTT assay, and the neurite outgrowth of those cells was evaluated by the computerized image-analysis system.
  
  
  RESULTS
  Compared with the control group, the absorbance values of cells survival in Y-27632 group increased 12.90% and 33.33% respectively after 72 and 96 hours culture. Y-27632 had no significant effect on the diameter of cultured retinal neurocytes. Compared with the control group, Y-27632 induced a stable improvement of neurite outgrowth of retinal neurocytes after 72 and 96 hours culture (P=0.001).
  
  
  CONCLUSIONS
  Y-27632 could promote the survival and neurite outgrowth of the early postnatal cultured retinal neurocytes.
authors.affiliation: Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Pei-Li
authors.initials: PL
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Zhong
keywords: Y-27632; cell culture; neurites; retinal neurocytes
references.citation: Mol Cell Neurosci. 2009 Oct;42(2):128-33
references.pmid: 19524675
references.citation: ACS Chem Neurosci. 2010 Mar 17;1(3):215-222
references.pmid: 20436925
references.citation: J Neurosci. 2010 May 19;30(20):6825-37
references.pmid: 20484625
references.citation: J Neurol Sci. 2005 Jun 15;233(1-2):43-7
references.pmid: 15949495
references.citation: Mol Cell Neurosci. 2007 Jul;35(3):409-23
references.pmid: 17493827
references.citation: EMBO J. 1996 Apr 15;15(8):1885-93
references.pmid: 8617235
references.citation: J Neurosci. 2004 Sep 29;24(39):8510-21
references.pmid: 15456825
references.citation: J Biol Chem. 2002 Sep 6;277(36):32820-9
references.pmid: 12091381
references.citation: J Neurosci. 2011 Feb 2;31(5):1664-75
references.pmid: 21289175
references.citation: J Neurosci. 2005 Feb 2;25(5):1113-21
references.pmid: 15689547
references.citation: Int J Ophthalmol. 2011;4(6):652-7
references.pmid: 22553739
references.citation: Neurosciences (Riyadh). 2010 Jul;15(3):152-8
references.pmid: 20831022
references.citation: Brain. 2008 Jan;131(Pt 1):250-63
references.pmid: 18063589
references.citation: J Neurosci. 2002 Aug 1;22(15):6570-7
references.pmid: 12151536
references.citation: J Neurosci. 2003 Feb 15;23(4):1416-23
references.pmid: 12598630