title: Two-day detection of infectious enteric and non-enteric adenoviruses by improved ICC-qPCR.
abstract: In order to provide a more suitable response to public health concerns, we improved the detection of infectious human adenoviruses in water by optimising the commonly used integrated cell culture-PCR method. Risk evaluation studies seek for rapid detection of infectious adenoviruses, including the enteric types 40 and 41 that are considered as the second most common agents of gastroenteritis in children next to rotaviruses. The here-employed 293A cell line used for infectious status assessment showed its ability to multiply adenoviruses including type 41. Two modifications were moreover applied to the workflow for viral detection. The first occurred at the nucleic acid extraction step performed directly on all infected cells, while the second was the application of real-time quantitative PCR as detection tool. All adaptations led to a 3-day reduction of the response delay and an improved sensitivity especially for the enteric adenoviral types. The infectious status of laboratory strain types 2 and 41 was demonstrated by a more than 2-log10 increase in genome quantity. These conclusions were confirmed and reinforced by the analysis of water samples applying the improved assay. Naturally occurring infectious adenoviruses were detected in wastewater and river water, within 2 days. Types belonging to the species human adenoviruses C and type 31 were observed, but the most frequently identified type was 41 (71 % of identified sequences, n = 34). This highlights the usefulness of our method for a wide range of types, and especially for the most prevalent and public health-relevant enteric adenoviruses.
authors.affiliation: Centre de Recherche Public-Gabriel Lippmann, Department of Environment and Agro-biotechnologies (EVA), 41 rue du Brill, 4422 Belvaux, Luxembourg. ogorzaly@lippmann.lu
authors.forename: Leslie
authors.initials: L
authors.lastname: Ogorzaly
authors.forename: Henry-Michel
authors.initials: HM
authors.lastname: Cauchie
authors.forename: Christian
authors.initials: C
authors.lastname: Penny
authors.forename: Aline
authors.initials: A
authors.lastname: Perrin
authors.forename: Christophe
authors.initials: C
authors.lastname: Gantzer
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bertrand
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000472:pathogenicity; D002460:Cell Line; D006801:Humans; D016133:Polymerase Chain Reaction