title: Screening of microRNA in patients with esophageal cancer at same tumor node metastasis stage with different prognoses.
abstract: Patients at the same pathological stage of esophageal cancer (EC) that received the same surgical therapy by the same surgeon may have distinct prognoses. The current study aimed to explore the possibility of differentially- expressed microRNAs (miRNAs) underlying this phenomenon. Samples were collected from EC patients at the same tumor node metastasis (TNM) stage but with different prognoses. Paracancerous normal tissues were taken as controls. The specimens were histopathologically analyzed. Differentially-expressed miRNAs were analyzed using real-time quantitative reverse transcription polymerase chain reaction. Compared with patients with poor prognosis, those with good prognosis exhibited 88 two-fold or more than two-fold increased miRNA fragments and 4 half-decreased miRNAs. The most noticeably up-regulated miRNAs included hsa-miR-31, hsa-miR- 196b, hsa-miR-652, hsa-miR-125a-5p, hsa-miR-146b, hsa-miR-200c, hsa-miR-23b, hsa-miR-29a, hsa-miR-186, hsa-miR-205, hsa-miR-376a, hsa-miR-410, hsa-miR-532-3p, and hsa-miR-598, whereas the most significantly- downregulated miRNAs were hsa-let-7e, hsa-miR-130b, and hsa-miR-103. EC patients at same TNM stage but with different prognoses show differentially-expressed miRNAs.
authors.affiliation: Department of Thoracic Surgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui, China.
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Zhao
authors.forename: Shang-Guo
authors.initials: SG
authors.lastname: Liu
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Ji
authors.forename: Bo
authors.initials: B
authors.lastname: Qi
authors.forename: Yi-Peng
authors.initials: YP
authors.lastname: Tao
authors.forename: Han-Chen
authors.initials: HC
authors.lastname: Li
authors.forename: Xiang-Nan
authors.initials: XN
authors.lastname: Wu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis
chemical_list: D035683:MicroRNAs