title: The biodiscovery potential of marine bacteria: an investigation of phylogeny and function.
abstract: A collection of marine bacteria isolated from a temperate coastal zone has been screened in a programme of biodiscovery. A total of 34 enzymes with biotechnological potential were screened in 374 isolates of marine bacteria. Only two enzymes were found in all isolates while the majority of enzyme activities were present in a smaller proportion of the isolates. A cluster analysis demonstrated no significant correlation between taxonomy and enzyme function. However, there was evidence of co-occurrence of some enzyme activity in the same isolate. In this study marine Proteobacteria had a higher complement of enzymes with biodiscovery potential than Actinobacteria; this contrasts with the terrestrial environment where the Actinobacteria phylum is a proven source of enzymes with important industrial applications. In addition, a number of novel enzyme functions were more abundant in this marine culture collection than would be expected on the basis of knowledge from terrestrial bacteria. There is a strong case for future investigation of marine bacteria as a source for biodiscovery.
authors.affiliation: TU Bergakademie Freiberg, Institute of Biological Sciences, Leipziger Str. 29, 09599, Freiberg, Germany. Martin.Muehling@ioez.tu-freiberg.de
authors.forename: Martin
authors.initials: M
authors.lastname: Mühling
authors.forename: Ian
authors.initials: I
authors.lastname: Joint
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Willetts
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D001688:Biological Products / Q000302:isolation & purification / Q000378:metabolism; D016000:Cluster Analysis; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*
chemical_list: D001688:Biological Products; D004798:Enzymes
references.citation: Science. 2004 Apr 2;304(5667):66-74
references.pmid: 15001713
references.citation: Curr Opin Biotechnol. 2002 Aug;13(4):345-51
references.pmid: 12323357
references.citation: Curr Opin Biotechnol. 2004 Aug;15(4):285-90
references.pmid: 15296926
references.citation: Science. 2006 Jan 27;311(5760):496-503
references.pmid: 16439655
references.citation: Microb Biotechnol. 2010 Sep;3(5):564-75
references.pmid: 21255353
references.citation: ISME J. 2008 Apr;2(4):379-92
references.pmid: 18340335
references.citation: Microb Biotechnol. 2010 Sep;3(5):531-43
references.pmid: 20953417
references.citation: ISME J. 2012 Feb;6(2):298-308
references.pmid: 21850055
references.citation: Mar Biotechnol (NY). 2005 May-Jun;7(3):173-8
references.pmid: 15906116
references.citation: J Protein Chem. 1996 May;15(4):345-50
references.pmid: 8819010
references.citation: Arch Microbiol. 1995 Sep;164(3):165-72
references.pmid: 7545384
references.citation: Appl Environ Microbiol. 2007 Jul;73(14):4559-69
references.pmid: 17526795
references.citation: Biotechnol J. 2009 Dec;4(12):1725-39
references.pmid: 19844915
references.citation: Adv Biochem Eng Biotechnol. 2002;74:207-38
references.pmid: 11991180
references.citation: J Bacteriol. 1991 Jan;173(2):697-703
references.pmid: 1987160
references.citation: J Am Chem Soc. 2005 Dec 21;127(50):17594-5
references.pmid: 16351075
references.citation: PLoS One. 2008 Aug 22;3(8):e3042
references.pmid: 18725995
references.citation: Trends Microbiol. 2007 Nov;15(11):491-9
references.pmid: 17997312
references.citation: Eur J Biochem. 1978 May;86(1):175-86
references.pmid: 77775
references.citation: Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21219-23
references.pmid: 19926862
references.citation: J Mol Microbiol Biotechnol. 2009;16(1-2):25-37
references.pmid: 18957860
references.citation: Science. 2005 Apr 22;308(5721):554-7
references.pmid: 15845853
references.citation: Annu Rev Microbiol. 2001;55:485-529
references.pmid: 11544365
references.citation: Mar Drugs. 2011;9(4):478-99
references.pmid: 21731544
references.citation: Nature. 2008 Apr 3;452(7187):629-32
references.pmid: 18337718
references.citation: Microbiol Mol Biol Rev. 2000 Sep;64(3):573-606
references.pmid: 10974127