title: Light scattering methods for tracking gold nanoparticles aggregation induced by biotin-neutravidin interaction.
abstract: Dynamic and Static Light Scattering (DLS and SLS), Resonance Light Scattering (RLS) and angular-ratiometric methods have been used for investigating the aggregation of biotinylated bovine serum albumin gold nanoparticles induced by the interaction of biotin with its partner neutravidin. All the approaches have shown to be sensitive to the presence of neutravidin in solution at nM-concentrations. Changes in scattered light intensity dependence on the scattering angle (studied by SLS) and on the wavelength of the incident light (by RLS) have been observed. Predictions from Mie theory have enabled to connect these changes to the increase in the hydrodynamic size of the aggregates, quantified by DLS. The results here reported confirm the potentials of light scattering approach for realizing methods for analyte quantification and offer a good starting point for evaluating the limits and advantages of each method, which would further widen the use of light scattering approach in biosensing.
authors.affiliation: Biophysics and Nanoscience Centre, Dipartimento di Scienze Ecologiche e Biologiche, Università della Tuscia, Viterbo, Italy. delfino@unitus.it
authors.forename: Ines
authors.initials: I
authors.lastname: Delfino
mesh_terms: D000818:Animals; D001360:Avidin / Q000737:chemistry / Q000378:metabolism*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D019921:Biotinylation; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D057446:Hydrodynamics; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism
chemical_list: C093483:neutravidin; D001360:Avidin; D012710:Serum Albumin, Bovine; D001710:Biotin; D006046:Gold