title: Evaluation of a combined linear-nonlinear approach for column characterization using modern alkaline-stable columns as model.
abstract: This study investigates if deeper understanding is achieved when combining nonlinear and linear chromatographic column characterization methods. As test systems, two hybrid columns (Phenomenex Gemini-NX C18 and Kromasil Eternity C18) and one classic one (Kromasil-C18) were selected. The nonlinear methods were based on firm adsorption theory and involved determination of adsorption isotherms followed by calculations with a new numerical tool, adsorption energy distribution, on probe components at different pH values. The linear methods involved the hydrophobic subtraction model and selected probe components retention factors as a function of pH. The combined analysis indicated that both complementary and confirmative information can be achieved regarding the actual model systems.
authors.affiliation: Department of Chemistry-BMC, Uppsala University, Uppsala, Sweden.
authors.forename: Torgny
authors.initials: T
authors.lastname: Undin
authors.forename: Jörgen
authors.initials: J
authors.lastname: Samuelsson
authors.forename: Anders
authors.initials: A
authors.lastname: Törncrona
authors.forename: Torgny
authors.initials: T
authors.lastname: Fornstedt