title: Crystallization and preliminary crystallographic analysis of Axe2, an acetylxylan esterase from Geobacillus stearothermophilus.
abstract: Acetylxylan esterases are part of the hemi-cellulolytic system of many microorganisms which utilize plant biomass for growth. Xylans, which are polymeric sugars that constitute a significant part of the plant biomass, are usually substituted with acetyl side groups attached at position 2 or 3 of the xylose backbone units. Acetylxylan esterases hydrolyse the ester linkages of the xylan acetyl groups and thus improve the ability of main-chain hydrolysing enzymes to break down the sugar backbone units. As such, these enzymes play an important part in the hemi-cellulolytic utilization system of many microorganisms that use plant biomass for growth. Interest in the biochemical characterization and structural analysis of these enzymes stems from their numerous potential biotechnological applications. An acetylxylan esterase (Axe2) of this type from Geobacillus stearothermophilus T-6 has recently been cloned, overexpressed, purified, biochemically characterized and crystallized. One of the crystal forms obtained (RB1) belonged to the tetragonal space group I422, with unit-cell parameters a = b = 110.2, c = 213.1 Å. A full diffraction data set was collected to 1.85 Å resolution from flash-cooled crystals of the wild-type enzyme at 100 K using synchrotron radiation. A selenomethionine derivative of Axe2 has also been prepared and crystallized for single-wavelength anomalous diffraction experiments. The crystals of the selenomethionine-derivatized Axe2 appeared to be isomorphous to those of the wild-type enzyme and enabled the measurement of a full 1.85 Å resolution diffraction data set at the selenium absorption edge and a full 1.70 Å resolution data set at a remote wavelength. These data are currently being used for three-dimensional structure determination of the Axe2 protein.
authors.affiliation: Institute of Chemistry and the Laboratory for Structural Chemistry and Biology, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Shifra
authors.initials: S
authors.lastname: Lansky
authors.forename: Onit
authors.initials: O
authors.lastname: Alalouf
authors.forename: Vered
authors.initials: V
authors.lastname: Solomon
authors.forename: Anat
authors.initials: A
authors.lastname: Alhassid
authors.forename: Lata
authors.initials: L
authors.lastname: Govada
authors.forename: Naomi E
authors.initials: NE
authors.lastname: Chayen
authors.forename: Naomi E
authors.initials: NE
authors.lastname: Chayan
authors.forename: Hassan
authors.initials: H
authors.lastname: Belrhali
authors.forename: Yuval
authors.initials: Y
authors.lastname: Shoham
authors.forename: Gil
authors.initials: G
authors.lastname: Shoham
mesh_terms: D000115:Acetylesterase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D001411:Geobacillus stearothermophilus / Q000201:enzymology*
chemical_list: D000115:Acetylesterase; C081851:acetylxylan esterase
keywords: Geobacillus stearothermophilus; SAD data collection; selenium absorption edge; serine esterases; xylan esterases
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