title: Eplerenone promotes alternative activation in human monocyte-derived macrophages.
abstract: BACKGROUND
  In this study, we have analyzed the response of human monocyte-derived macrophages to mineralocorticoid axis modulators.
  
  
  METHODS
  Human monocyte-derived macrophages were incubated with aldosterone alone, eplerenone alone, and the combination of aldosterone and eplerenone. The analyzed variables were nitric oxide and reactive oxygen species production, and the gene and protein expression of inducible nitric oxide synthase, arginase I, and mannose receptor.
  
  
  RESULTS
  We showed that aldosterone promotes a classic inflammatory response in macrophages, whereas its antagonist, eplerenone, attenuates aldosterone-induced activity.
  
  
  CONCLUSIONS
  Eplerenone did not quantitatively weaken the response of macrophages to aldosterone but instead qualitatively changed their behavior.
authors.affiliation: Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia, Medyków 18, PL 40-752 Katowice, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Łabuzek
authors.forename: Sebastian
authors.initials: S
authors.lastname: Liber
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Bułdak
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Machnik
authors.forename: Justyna
authors.initials: J
authors.lastname: Liber
authors.forename: Bogusław
authors.initials: B
authors.lastname: Okopień
mesh_terms: D000293:Adolescent; D000328:Adult; D000450:Aldosterone / Q000008:administration & dosage / Q000494:pharmacology*; D001119:Arginase / Q000235:genetics; D002478:Cells, Cultured; D000077545:Eplerenone; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000235:genetics; D000451:Mineralocorticoid Receptor Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics; D013148:Spironolactone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D055815:Young Adult
chemical_list: D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D000451:Mineralocorticoid Receptor Antagonists; D017382:Reactive Oxygen Species; D011956:Receptors, Cell Surface; D013148:Spironolactone; D009569:Nitric Oxide; D000450:Aldosterone; D000077545:Eplerenone; D052247:Nitric Oxide Synthase Type II; C574619:ARG1 protein, human; D001119:Arginase