title: Characterization of MYC-induced tumorigenesis by in situ lipid profiling.
abstract: We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.
authors.forename: Richard H
authors.initials: RH
authors.lastname: Perry
authors.forename: David I
authors.initials: DI
authors.lastname: Bellovin
authors.forename: Emelyn H
authors.initials: EH
authors.lastname: Shroff
authors.forename: Ali I
authors.initials: AI
authors.lastname: Ismail
authors.forename: Tahera
authors.initials: T
authors.lastname: Zabuawala
authors.forename: Dean W
authors.initials: DW
authors.lastname: Felsher
authors.forename: Richard N
authors.initials: RN
authors.lastname: Zare
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008055:Lipids; D016271:Proto-Oncogene Proteins c-myc
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