title: Plasmid-borne type E neurotoxin gene clusters in Clostridium botulinum strains.
abstract: A collection of 36 Clostridium botulinum type E strains was examined by pulsed-field gel electrophoresis (PFGE) and Southern hybridization with probes targeted to botE and orfX1 in the neurotoxin gene cluster. Three strains were found to contain neurotoxin subtype E1 gene clusters in large plasmids of about 146 kb in size.
authors.affiliation: Department of Food Hygiene and Environmental Health, Centre of Excellence in Microbial Food Safety Research, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hannamari
authors.initials: H
authors.lastname: Hintsa
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Hannu
authors.initials: H
authors.lastname: Korkeala
authors.forename: Miia
authors.initials: M
authors.lastname: Lindström
mesh_terms: D015139:Blotting, Southern; D003014:Clostridium botulinum / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005810:Multigene Family / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D013744:Tetanus Toxin / Q000235:genetics*
chemical_list: D017931:DNA Primers; D013744:Tetanus Toxin
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