title: Differentiation of tannin-containing herbal drugs by HPLC fingerprints.
abstract: A new HPLC system coupled with multiple detectors - Diode array detector (DAD), fluorescence detector (FLD), electrochemical amperometric detector (ADC) and mass spectrometry detector (MSD) was developed for the characterization and differentiation of tannin-containing herbal drugs included in The European Pharmacopoeia. The HPLC separation system consisted of an Agilent ZORBAX Eclipse XDB C18 column and a gradient water and methanol as the mobile phase which was kept at a flow rate of 0.3 mL x min(-1). Four kinds of detectors were connected by a micro-splitter valve and simultaneously recorded the response of each analytical sample. Thirty-one samples from eight kinds of tannin-containing drugs were measured using this HPLC system and their signals from all detectors were comprehensively processed via principal component analysis (PCA). The statistic result demonstrates that thirty-one batches from different herbal drugs can be reasonably identified and systematically classified by their chemical fingerprints. The proposed multi-detector HPLC method aided by chemometrics not only offers a new pattern for the study of tannin-containing herbs, but also provides a useful foundation for quality control of herbal medicines.
authors.affiliation: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Wu
authors.forename: S H
authors.initials: SH
authors.lastname: Hansen
authors.forename: C
authors.initials: C
authors.lastname: Cornett
authors.forename: C
authors.initials: C
authors.lastname: Møller
authors.forename: Pingfan
authors.initials: P
authors.lastname: Lai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004563:Electrochemistry; D013058:Mass Spectrometry; D028321:Plant Preparations / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D025341:Principal Component Analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013634:Tannins / Q000032:analysis*
chemical_list: D028321:Plant Preparations; D012996:Solutions; D013634:Tannins