title: Supramolecular autoregulation.
abstract: Enzyme activity in biological systems is often governed by control mechanisms in which the catalytic properties are made sensitive or insensitive to differences in enzyme or substrate concentration. Here, we report the first supramolecular system where the catalytic activity is made concentration independent through the use of newly designed inhibitor molecules. The precise concentration dependence of coupled supramolecular equilibriums between free catalyst, inhibited catalyst, active inhibitor, and inactive inhibitor allows to keep the concentration of free catalyst at 1 mM in a broad concentration range, yielding an autoregulated catalytic system.
authors.affiliation: Institute for Complex Molecular Systems and Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, The Netherlands.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodríguez-Llansola
authors.forename: E W
authors.initials: EW
authors.lastname: Meijer
mesh_terms: D019256:Cadmium Chloride / Q000737:chemistry; D002384:Catalysis; D015195:Drug Design; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004798:Enzymes / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D015233:Models, Statistical; D009287:Naphthyridines / Q000737:chemistry; D011744:Pyrimidinones / Q000737:chemistry
chemical_list: C516002:2,7-diamido-1,8-naphthyridine; C029046:2-ureido-4(3H)-pyrimidinone; D004791:Enzyme Inhibitors; D004798:Enzymes; D009287:Naphthyridines; D011744:Pyrimidinones; D019256:Cadmium Chloride