title: Histologic evaluation of chitosan as an accelerator of bone regeneration in microdrilled rat tibias.
abstract: BACKGROUND
  Chitosan compounds have been shown to be suitable bone replacement materials. To evaluate the accelerating effects of chitosan on the bone regeneration process and assessing its histopathological adverse effects, we conducted this study on rat tibias.
  
  
  METHODS
  In a laboratory experimental study, micro-drilled bone defects were created in the upper tibia of each leg in 15 adult male rats. The defect in the right leg, filled by the chitosan powder, was compared with the untreated defect in the left leg in each rat at 1, 2, and 4 weeks after surgery. Bone repair and inflammation in each specimen was blindly graded by a pathologist. Reaction to the foreign body and the amount of the remaining chitosan were studied in chitosan-treated specimens at the three stages of the study.
  
  
  RESULTS
  Bone repair was significantly faster in the chitosan group, 1 week (P = 0.01) and 4 weeks (P = 0.038) after surgery, while the difference was not significant at the 2-week stage (P = 0.197) between chitosan and control groups. Chitosan-induced inflammation was not significant in any stage of the study. Reaction to the foreign body was seen in one case at 2 weeks and one case at 4 weeks postoperation.
  
  
  CONCLUSIONS
  Chitosan significantly accelerated the bone regeneration process in rat tibias. Regarding its biocompatibility and osteoinductivity, it can be studied as a biomaterial in human bone healing.
authors.affiliation: Department of Maxillofacial Radiology, Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ezoddini-Ardakani
authors.forename: Alireza
authors.initials: A
authors.lastname: Navabazam
authors.forename: Farhad
authors.initials: F
authors.lastname: Fatehi
authors.forename: Mohamad
authors.initials: M
authors.lastname: Danesh-Ardekani
authors.forename: Somayyeh
authors.initials: S
authors.lastname: Khadem
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Rouhi
keywords: Bone regeneration; chitosan; rat
references.citation: Osteoarthritis Cartilage. 2007 Mar;15(3):316-27
references.pmid: 17008111
references.citation: J Biomed Mater Res A. 2009 Mar 1;88(3):569-80
references.pmid: 18306317
references.citation: Pharm Res. 1996 Apr;13(4):588-93
references.pmid: 8710751
references.citation: J Biomed Mater Res B Appl Biomater. 2009 Jul;90(1):461-6
references.pmid: 19145627
references.citation: Artif Organs. 1992 Dec;16(6):568-76
references.pmid: 1482326
references.citation: Small. 2008 Feb;4(2):240-6
references.pmid: 18205152
references.citation: Biomaterials. 1997 Mar;18(5):375-81
references.pmid: 9061177
references.citation: J Biomed Mater Res A. 2010 Apr;93(1):107-14
references.pmid: 19536827
references.citation: J Biomed Mater Res B Appl Biomater. 2012 Jul;100(5):1435-43
references.pmid: 22287502
references.citation: Biomater Artif Cells Artif Organs. 1990;18(1):1-24
references.pmid: 2185854
references.citation: Biomaterials. 1994 Oct;15(13):1075-81
references.pmid: 7888578
references.citation: J Invest Surg. 2010 Jun;23(3):134-41
references.pmid: 20590384
references.citation: Biomaterials. 2005 Oct;26(30):5983-90
references.pmid: 15894370
references.citation: Biomaterials. 2001 Jan;22(2):135-50
references.pmid: 11101158
references.citation: Osteoarthritis Cartilage. 2007 Jan;15(1):78-89
references.pmid: 16895758
references.citation: J Appl Biomater. 1991 Fall;2(3):187-208
references.pmid: 10149083
references.citation: Mar Drugs. 2010 Aug 02;8(8):2252-66
references.pmid: 20948907
references.citation: J Periodontol. 2005 Oct;76(10):1778-84
references.pmid: 16253101
references.citation: J Colloid Interface Sci. 2012 Jan 15;366(1):224-232
references.pmid: 22024371
references.citation: Biomaterials. 2002 Nov;23(21):4167-76
references.pmid: 12194519
references.citation: Acta Biomater. 2009 May;5(4):1182-97
references.pmid: 19121983
references.citation: Cell Prolif. 2011 Dec;44(6):537-49
references.pmid: 22011046
references.citation: J Biomed Mater Res A. 2005 Nov 1;75(2):275-82
references.pmid: 16044404
references.citation: Biomaterials. 1993 Oct;14(12):939-44
references.pmid: 8268386
references.citation: J Control Release. 2002 Jan 17;78(1-3):187-97
references.pmid: 11772460
references.citation: J Craniofac Surg. 2005 Mar;16(2):213-24; discussion 225-7
references.pmid: 15750417
references.citation: J Biomater Sci Polym Ed. 2012;23(1-4):425-38
references.pmid: 21255485