title: Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris.
abstract: Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H(57) and D(102) was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Ling
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Shaolei
authors.initials: S
authors.lastname: Teng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Kang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
mesh_terms: D019149:Bioreactors; D003198:Computer Simulation; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000235:genetics; D006860:Hydrogen Bonding; D008958:Models, Molecular; D010843:Pichia / Q000378:metabolism*; D011498:Protein Precursors / Q000008:administration & dosage / Q000494:pharmacology*; D013305:Streptomyces griseus / Q000737:chemistry / Q000201:enzymology*; D014357:Trypsin / Q000096:biosynthesis*; D014362:Trypsinogen / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D014362:Trypsinogen; D014357:Trypsin
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