title: Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species.
abstract: Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species.
authors.affiliation: Programa de Pós-Graduação em Biotecnologia e Recursos Naturais, Universidade do Estado do Amazonas, Manaus, AM, Brasil.
authors.forename: P F
authors.initials: PF
authors.lastname: Cruz
authors.forename: J S
authors.initials: JS
authors.lastname: Batista
authors.forename: K M
authors.initials: KM
authors.lastname: Formiga
authors.forename: M S
authors.initials: MS
authors.lastname: Rafael
authors.forename: W P
authors.initials: WP
authors.lastname: Tadei
authors.forename: J M M
authors.initials: JM
authors.lastname: Santos
mesh_terms: D000483:Alleles; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001938:Brazil; D004247:DNA / Q000235:genetics; D005828:Genetics, Population; D006801:Humans; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D004247:DNA