title: Late gadolinium enhancement in the assessment of the infarcted mouse heart: a longitudinal comparison with manganese-enhanced MRI.
abstract: OBJECTIVE
  To evaluate late gadolinium-enhanced (LGE) assessment of infarct size, a comparison with manganese-enhanced magnetic resonance imaging (MEMRI), and histology was performed in a permanent infarction model in the mouse at the acute and chronic stage.
  
  
  METHODS
  In a paired fashion at the acute and chronic stage after infarction (3-4 days and 21 days, respectively), LGE and MEMRI was performed using a self-gated fast low flip angle shot (FLASH). Infarct size was evaluated as the enhanced area relative to the complete myocardial wall area in a mid-ventricular slice. Paired comparisons were made between contrast agents and between timepoints, as well as to histology.
  
  
  RESULTS
  At the acute stage, LGE delineated a larger infarct size as compared to both MEMRI and histology. Infarct size from LGE decreased from the acute to chronic stage, a temporal development not seen with MEMRI. At the chronic stage, no significant differences in infarct size were found between the methods.
  
  
  CONCLUSIONS
  This study indicates an overenhancement of infarct size when using LGE, supported by an initial overestimation at the acute stage and a temporal decrease in infarct size from the acute to chronic stage, as compared to infarct size from MEMRI.
authors.affiliation: Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; MILab, Trondheim, Norway.
authors.forename: Kristine
authors.initials: K
authors.lastname: Skårdal
authors.forename: Natale P L
authors.initials: NP
authors.lastname: Rolim
authors.forename: Olav
authors.initials: O
authors.lastname: Haraldseth
authors.forename: Pål Erik
authors.initials: PE
authors.lastname: Goa
authors.forename: Marte
authors.initials: M
authors.lastname: Thuen
mesh_terms: D000465:Algorithms; D000818:Animals; D055424:Cardiac-Gated Imaging Techniques / Q000379:methods*; D003287:Contrast Media / Q000008:administration & dosage; D005260:Female; D019786:Gadolinium DTPA / Q000008:administration & dosage*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008137:Longitudinal Studies; D015636:Magnesium Chloride / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003287:Contrast Media; D015636:Magnesium Chloride; C064925:gadodiamide; D019786:Gadolinium DTPA
keywords: contrast enhancement; infarct size; late gadolinium enhancement; manganese enhanced MRI; mice; permanent infarction