title: Improvement of a gas chromatographic method for the analysis of iminosugars and other bioactive carbohydrates.
abstract: The analysis of derivatised iminosugars and other bioactive low molecular weight carbohydrates present at low concentrations in different vegetable extracts (hyacinth, mulberry and buckwheat) required the improvement of a previously developed gas chromatographic method. Among the different parameters optimized, the temperature of the injection port, evaluated for the first time, resulted to be the most important. Thus, 240 °C was chosen as a tradeoff to achieve the required volatility, to avoid degradation and to provide symmetric peaks for bioactive carbohydrates highly retained such as glycosyl inositols. GC-MS operating under selective ion monitoring (SIM) acquisition mode and GC-FID provided limits of detection and quantitation for the target compounds of 0.2 and 0.7 ng g(-1) and of 1 and 3 ng g(-1) on average, respectively. Therefore, both methodologies could be considered appropriate to extend the range of quantitation of these bioactives.
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Rodríguez-Sánchez
authors.forename: A C
authors.initials: AC
authors.lastname: Soria
authors.forename: A I
authors.initials: AI
authors.lastname: Ruiz-Matute
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D050111:Imino Sugars / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D050111:Imino Sugars