title: Structure of the hypothetical DUF1811-family protein GK0453 from Geobacillus kaustophilus HTA426.
abstract: The crystal structure of a conserved hypothetical protein, GK0453, from Geobacillus kaustophilus has been determined to 2.2 Å resolution. The crystal belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 75.69, c = 64.18 Å. The structure was determined by the molecular-replacement method and was refined to a final R factor of 22.6% (R(free) = 26.3%). Based on structural homology, the GK0453 protein possesses two independent binding sites and hence it may simultaneously interact with two proteins or with a protein and a nucleic acid.
authors.affiliation: Department of Biophysics, National Institute of Mental Health and Neuro Sciences, Bangalore 560 029, India.
authors.forename: Balasundaram
authors.initials: B
authors.lastname: Padmanabhan
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Antonyuk
authors.forename: Richard W
authors.initials: RW
authors.lastname: Strange
authors.forename: S Samar
authors.initials: SS
authors.lastname: Hasnain
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Bessho
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D056506:Geobacillus / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity; D040681:Structural Homology, Protein
chemical_list: D001426:Bacterial Proteins
keywords: DUF1811; Geobacillus kaustophilus; helix–turn–helix motif; β-barrel domain
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