title: Odorant metabolism catalyzed by olfactory mucosal enzymes influences peripheral olfactory responses in rats.
abstract: A large set of xenobiotic-metabolizing enzymes (XMEs), such as the cytochrome P450 monooxygenases (CYPs), esterases and transferases, are highly expressed in mammalian olfactory mucosa (OM). These enzymes are known to catalyze the biotransformation of exogenous compounds to facilitate elimination. However, the functions of these enzymes in the olfactory epithelium are not clearly understood. In addition to protecting against inhaled toxic compounds, these enzymes could also metabolize odorant molecules, and thus modify their stimulating properties or inactivate them. In the present study, we investigated the in vitro biotransformation of odorant molecules in the rat OM and assessed the impact of this metabolism on peripheral olfactory responses. Rat OM was found to efficiently metabolize quinoline, coumarin and isoamyl acetate. Quinoline and coumarin are metabolized by CYPs whereas isoamyl acetate is hydrolyzed by carboxylesterases. Electro-olfactogram (EOG) recordings revealed that the hydroxylated metabolites derived from these odorants elicited lower olfactory response amplitudes than the parent molecules. We also observed that glucurono-conjugated derivatives induced no olfactory signal. Furthermore, we demonstrated that the local application of a CYP inhibitor on rat olfactory epithelium increased EOG responses elicited by quinoline and coumarin. Similarly, the application of a carboxylesterase inhibitor increased the EOG response elicited by isoamyl acetate. This increase in EOG amplitude provoked by XME inhibitors is likely due to enhanced olfactory sensory neuron activation in response to odorant accumulation. Taken together, these findings strongly suggest that biotransformation of odorant molecules by enzymes localized to the olfactory mucosa may change the odorant's stimulating properties and may facilitate the clearance of odorants to avoid receptor saturation.
authors.affiliation: CNRS, UMR6265, Centre des Sciences du Goût et de l'Alimentation, Dijon, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Thiebaud
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Veloso Da Silva
authors.forename: Ingrid
authors.initials: I
authors.lastname: Jakob
authors.forename: Gilles
authors.initials: G
authors.lastname: Sicard
authors.forename: Joëlle
authors.initials: J
authors.lastname: Chevalier
authors.forename: Franck
authors.initials: F
authors.lastname: Ménétrier
authors.forename: Olivier
authors.initials: O
authors.lastname: Berdeaux
authors.forename: Yves
authors.initials: Y
authors.lastname: Artur
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Heydel
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Le Bon
mesh_terms: D000818:Animals; D055162:Biocatalysis / Q000187:drug effects; D003374:Coumarins / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D009812:Odorants; D009831:Olfactory Mucosa / Q000201:enzymology* / Q000378:metabolism; D055696:Olfactory Perception / Q000187:drug effects; D000439:Pentanols / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D015363:Quinolones / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D003374:Coumarins; D004791:Enzyme Inhibitors; D000439:Pentanols; D015363:Quinolones; D015262:Xenobiotics; C030123:coumarin; C020377:isoamyl acetate
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