title: Post-transcriptional gene silencing of the gene encoding aldolase from soybean cyst nematode by transformed soybean roots.
abstract: Plant parasitic nematodes cause approximately 157 billion US dollars in losses worldwide annually. The soybean cyst nematode (SCN), Heterodera glycines, is responsible for an estimated one billion dollars in losses to the US farmer each year. A promising new approach for control of plant parasitic nematode control is gene silencing. We tested this approach by silencing the SCN gene HgALD, encoding fructose-1,6-diphosphate aldolase. This enzyme is important in the conversion of glucose into energy and may be especially important in actin-based motility during parasite invasion of its host. An RNAi construct targeted to silence HgALD was transformed into soybean roots of composite plants to examine its efficacy to reduce the development of females formed by SCN. The number of mature females on roots transformed with the RNAi construct designed to silence the HgALD gene was reduced by 58%. These results indicate that silencing the aldolase gene of SCN +can greatly decrease the number of female SCN reaching maturity, and it is a promising step towards broadening resistance of plants against plant-parasitic nematodes.
authors.affiliation: USDA-ARS, Soybean Genomic and Improvement Laboratory, 10300 Baltimore Ave., Beltsville, MD 20705, United States.
authors.forename: Reham M
authors.initials: RM
authors.lastname: Youssef
authors.forename: Kyung-Hwan
authors.initials: KH
authors.lastname: Kim
authors.forename: Sanaa A
authors.initials: SA
authors.lastname: Haroon
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Matthews
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D016960:Agrobacterium tumefaciens / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D015536:Down-Regulation; D018274:Electroporation; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000592:standards; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D010572:Pest Control, Biological / Q000379:methods; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D016415:Sequence Alignment; D013025:Glycine max / Q000235:genetics / Q000469:parasitology*; D014170:Transformation, Genetic; D014431:Tylenchoidea / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D010088:Oxidoreductases; C022398:phytoene dehydrogenase; D005634:Fructose-Bisphosphate Aldolase