title: Pusillimonas sp. 5HP degrading 5-hydroxypicolinic acid.
abstract: A bacterial strain 5HP capable of degrading and utilizing 5-hydroxypicolinic acid as the sole source of carbon and energy was isolated from soil. In addition, the isolate 5HP could also utilize 3-hydroxypyridine and 3-cyanopyridine as well as nicotinic, benzoic and p-hydroxybenzoic acids for growth in the basic salt media. On the basis of 16S rRNA gene sequence analysis, the isolate 5HP was shown to belong to the genus Pusillimonas. Both the bioconversion analysis using resting cells and the enzymatic assay showed that the degradation of 5-hydroxypicolinic acid, 3-hydroxypyridine and nicotinic acid was inducible and proceeded via formation of the same metabolite, 2,5-dihydroxypyridine. The activity of a novel enzyme, 5-hydroxypicolinate 2-monooxygenase, was detected in the cell-free extracts prepared from 5-hydroxypicolinate-grown cells. The enzyme was partially purified and was shown to catalyze the oxidative decarboxylation of 5-hydroxypicolinate to 2,5-dihydroxypyridine. The activity of 5-hydroxypicolinate 2-monooxygenase was dependent on O2, NADH and FAD.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Mokslininku 12, LT-08662, Vilnius, Lithuania.
authors.forename: Laimonas
authors.initials: L
authors.lastname: Karvelis
authors.forename: Renata
authors.initials: R
authors.lastname: Gasparavičiūtė
authors.forename: Algirdas
authors.initials: A
authors.lastname: Klimavičius
authors.forename: Regina
authors.initials: R
authors.lastname: Jančienė
authors.forename: Jonita
authors.initials: J
authors.lastname: Stankevičiūtė
authors.forename: Rolandas
authors.initials: R
authors.lastname: Meškys
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D019817:Benzoic Acid / Q000378:metabolism; D020562:Betaproteobacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000302:isolation & purification / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009525:Niacin / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010226:Parabens / Q000378:metabolism; D010802:Phylogeny; D010848:Picolinic Acids / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D010226:Parabens; D010848:Picolinic Acids; D011725:Pyridines; D012336:RNA, Ribosomal, 16S; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D009525:Niacin; C023500:3-hydroxypyridine; D019817:Benzoic Acid; D006899:Mixed Function Oxygenases; C038193:4-hydroxybenzoic acid; D010100:Oxygen; C020729:3-cyanopyridine; C065452:2,5-dihydroxypyridine