title: A novel PCR assay for quantification of HIV-1 RNA.
abstract: Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.
authors.affiliation: Department of Medicine, Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Shan
authors.forename: S Alireza
authors.initials: SA
authors.lastname: Rabi
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Laird
authors.forename: Evelyn E
authors.initials: EE
authors.lastname: Eisele
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Margolick
authors.forename: Robert F
authors.initials: RF
authors.lastname: Siliciano
mesh_terms: D017931:DNA Primers / Q000235:genetics; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
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