title: Esophageal cancer alters the expression of nuclear pore complex binding protein Hsc70 and eIF5A-1.
abstract: Nuclear pore complex (NPC) is the only corridor for macromolecules exchange between nucleus and cytoplasm. NPC and its components, nucleoporins, play important role in the diverse physiological processes including macromolecule exchange, chromosome segregation, apoptosis and gene expression. Recent reports also suggest involvement of nucleoporins in carcinogenesis. Applying proteomics, we analyzed expression pattern of the NPC components in a newly established esophageal cancer cell line from Persia (Iran), the high-risk region for esophageal cancer. Our results indicate overexpression of Hsc70 and downregulation of subunit alpha type-3 of proteasome, calpain small subunit 1, and eIF5A-1. Among these proteins, Hsc70 and eIF5A-1 are in direct interaction with NPC and involved in the nucleocytoplasmic exchange. Hsc70 plays a critical role as a chaperone in the formation of a cargo-receptor complex in nucleocytoplasmic transport. On the other hand, it is an NPC-associated protein that binds to nucleoporins and contributes in recycling of the nucleocytoplasmic transport receptors in mammals and affects transport of proteins between nucleus and cytoplasm. The other nuclear pore interacting protein: eIF5A-1 binds to the several nucleoporins and participates in nucleocytoplasmic transport. Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. mehdimoghani@yahoo.com
authors.forename: Mehdi
authors.initials: M
authors.lastname: Moghanibashi
authors.forename: Ferdous
authors.initials: F
authors.lastname: Rastgar Jazii
authors.forename: Zahra-Soheila
authors.initials: ZS
authors.lastname: Soheili
authors.forename: Maryam
authors.initials: M
authors.lastname: Zare
authors.forename: Aliasghar
authors.initials: A
authors.lastname: Karkhane
authors.forename: Kazem
authors.initials: K
authors.lastname: Parivar
authors.forename: Parisa
authors.initials: P
authors.lastname: Mohamadynejad
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D004938:Esophageal Neoplasms / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D022022:Nuclear Pore / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000378:metabolism*; D011485:Protein Binding; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D050883:HSC70 Heat-Shock Proteins; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C494371:HSPA8 protein, human
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