title: Single cell gene expression analysis of pluripotent stem cells.
abstract: Analyzing gene expression profiles from cells en masse provides an average profile for the population which may obscure differences in individual cells. Using an optimized workflow for qRT-PCR, gene expression profiles of undifferentiated pluripotent stem cells reveal distinct gene expression profiles for individual cells, and a large expression level range of almost every gene. Importantly, this technique allows for the identification and characterization of small subpopulations.
authors.affiliation: Molecular And Cell biology, Life Technologies, Austin, TX, USA.
authors.forename: Ronald V
authors.initials: RV
authors.lastname: Abruzzese
authors.forename: Richard A
authors.initials: RA
authors.lastname: Fekete
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D039904:Pluripotent Stem Cells / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059010:Single-Cell Analysis; D059467:Transcriptome