title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis.
abstract: Tryparedoxin peroxidase (TXNPx) is an essential constituent of the main enzymatic scavenger system for reactive oxygen species (ROS) in trypanosomatids. Genetic studies have demonstrated the importance of this system for the development and virulence of these parasites, representing a potential target for the discovery of new trypanocidal drugs. In this work, the mitochondrial TXNPx from Leishmania braziliensis was cloned, overexpressed, purified and crystallized. The crystals diffracted to 3.3 Å resolution and belonged to space group P4(2)2(1)2, with unit-cell parameters a = b = 131.8, c = 44.4 Å. These studies will contribute to a better understanding of the molecular mechanisms involved in ROS detoxification by trypanosomatids.
authors.affiliation: Laboratório Nacional de Biociências, Laboratório Nacional de Luz Síncrotron, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP 13083-970, Brazil.
authors.forename: Mariana Abrahão Bueno
authors.initials: MA
authors.lastname: de Morais
authors.forename: Tatiana de Arruda Campos Brasil
authors.initials: Tde A
authors.lastname: de Souza
authors.forename: Mario Tyago
authors.initials: MT
authors.lastname: Murakami
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D007892:Leishmania braziliensis / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification; D015800:Protozoan Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D015800:Protozoan Proteins; D010544:Peroxidases; C110959:tryparedoxin peroxidase
keywords: Leishmania braziliensis; tryparedoxin peroxidase
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