title: In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus.
abstract: BACKGROUND
  Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster differentiation 81 CD81, the main receptor for the virus in hepatocytes, although this receptor protein has been found in megakaryocytes. Still, it is not clear if HCV interacts with platelets directly or if this interaction is a consequence of its association with megakaryocytes. The aim of this study was to evaluate the interaction of HCV with platelets from non-infected individuals, after in vitro exposure to the virus.
  
  
  METHODS
  Platelets obtained from 50 blood donors not infected by HCV were incubated in vitro at 37°C for 48h with serum containing 100,000IU∕mL of genotype 1 HCV. After incubation, RNA extracted from the platelets was assayed for the presence of HCV by reverse transcription – polymerase chain reaction RT-PCR.
  
  
  RESULTS
  After incubation in the presence of virus, all samples of platelets showed HCV RNA.
  
  
  CONCLUSIONS
  The results demonstrate that, in vitro, the virus interacts with platelets despite the absence of the receptor CD81, suggesting that other molecules could be involved in this association.
authors.affiliation: Laboratório de Biologia Molecular do Hemocentro de Botucatu, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista -UNESP, Botucatu, SP, Brasil.
authors.forename: Juliana Lara
authors.initials: JL
authors.lastname: Padovani
authors.forename: Silvia Maria
authors.initials: SM
authors.lastname: Corvino
authors.forename: Jan Felix
authors.initials: JF
authors.lastname: Drexler
authors.forename: Giovanni Faria
authors.initials: GF
authors.lastname: Silva
authors.forename: Maria Inês de Moura Campos
authors.initials: MI
authors.lastname: Pardini
authors.forename: Rejane Maria Tommasini
authors.initials: RM
authors.lastname: Grotto
mesh_terms: D000328:Adult; D001782:Blood Donors; D001792:Blood Platelets / Q000821:virology*; D005260:Female; D016174:Hepacivirus / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060225:Tetraspanin 28 / Q000032:analysis*
chemical_list: D012367:RNA, Viral; D060225:Tetraspanin 28