title: Relating centromeric topography in fixed human chromosomes to α-satellite DNA and CENP-B distribution.
abstract: Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of α-satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and α-satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes.
authors.affiliation: Department of Pathology, Schulich School of Medicine and Dentistry, London, Ont, Canada.
authors.forename: W A
authors.initials: WA
authors.lastname: Khan
authors.forename: R
authors.initials: R
authors.lastname: Chisholm
authors.forename: S
authors.initials: S
authors.lastname: Tadayyon
authors.forename: A
authors.initials: A
authors.lastname: Subasinghe
authors.forename: P
authors.initials: P
authors.lastname: Norton
authors.forename: J
authors.initials: J
authors.lastname: Samarabandu
authors.forename: L J
authors.initials: LJ
authors.lastname: Johnston
authors.forename: J H
authors.initials: JH
authors.lastname: Knoll
authors.forename: P K
authors.initials: PK
authors.lastname: Rogan
mesh_terms: D045744:Cell Line, Tumor; D002503:Centromere / Q000235:genetics / Q000378:metabolism*; D051936:Centromere Protein B / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D004276:DNA, Satellite / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008214:Lymphocytes / Q000166:cytology; D008677:Metaphase; D018625:Microscopy, Atomic Force; D016707:Tissue Fixation / Q000379:methods
chemical_list: C497926:CENPB protein, human; D051936:Centromere Protein B; D002843:Chromatin; D015342:DNA Probes; D004276:DNA, Satellite