title: DNA isolation from mammalian samples.
abstract: DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Ximeng
authors.initials: X
authors.lastname: Liu
authors.forename: Shuko
authors.initials: S
authors.lastname: Harada
mesh_terms: D000818:Animals; D002725:Chloroform / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D008322:Mammals; D016612:Paraffin Embedding; D019800:Phenol / Q000737:chemistry
chemical_list: D019800:Phenol; D002725:Chloroform; D004247:DNA