title: Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris.
abstract: The polysaccharides extracted by hot water and precipitated by 50% ethanol from the fruiting bodies of cultured Cordyceps militaris (W-CBP50) were introduced. Antioxidant activities of W-CBP50, W-CBP50 I (obtained from W-CBP50 purified by Sephadex G-100 chromatography) and W-CBP50 II (obtained from W-CBP50I purified by Sephadex G-200 chromatography) were evaluated and structural features of W-CBP50 II were analyzed. The polysaccharide W-CBP50 II was able to scavenge the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), while W-CBP50 and W-CBP50I could scavenge DPPH, hydroxyl and superoxide radical. Structural characteristics of W-CBP50 II were investigated using GPC, GC-MS, (1)H NMR and IR. The results showed that it was mainly composed of α-glucose, α-mannose, α-galactose and α-arabinose with α-type glycosidic linkage and the molecular weight of its main component was 8.97 kD.
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. lilychen77@163.com
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Chen
authors.forename: Guanghong
authors.initials: G
authors.lastname: Wu
authors.forename: Zhuolie
authors.initials: Z
authors.lastname: Huang
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D032661:Cordyceps / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008516:Medicine, Chinese Traditional; D008970:Molecular Weight; D010851:Picrates / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry; D014574:Uronic Acids / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D010851:Picrates; D014574:Uronic Acids; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl