title: Circulating miRNA profile in HCV infected serum: novel insight into pathogenesis.
abstract: Changes in circulating miRNA profiles have been associated with different diseases. Here we demonstrate the circulating miRNA profile in serum of HCV infected individuals using a microRNA array that profiles the expression of 940 miRNAs. Serum samples from two HCV genotype - 1 and two HCV genotype - 3 infected individuals were compared with healthy controls. Expression levels of miR-134, miR-198, miR-320c and miR-483-5p that were commonly upregulated in case of both genotypes were validated in 36 individual patient serum samples. Serum miR-134, miR-320c and miR-483-5p were significantly upregulated during HCV infection. miR-320c and miR-483-5p were also upregulated in HCV- JFH1 infected cells and cell culture supernatant. Pathway analysis of putative target genes of these miRNAs indicated involvement of PI3K-Akt, NFKB and MAPK signaling pathways. Results revealed novel insights on the role of circulating miRNAs in mediating pathogenesis in HCV-infected cells.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
authors.forename: Shivaprasad
authors.initials: S
authors.lastname: Shwetha
authors.forename: K
authors.initials: K
authors.lastname: Gouthamchandra
authors.forename: Madhavi
authors.initials: M
authors.lastname: Chandra
authors.forename: B
authors.initials: B
authors.lastname: Ravishankar
authors.forename: M N
authors.initials: MN
authors.lastname: Khaja
authors.forename: Saumitra
authors.initials: S
authors.lastname: Das
mesh_terms: D016174:Hepacivirus / Q000235:genetics* / Q000472:pathogenicity*; D006526:Hepatitis C / Q000097:blood* / Q000235:genetics / Q000821:virology*; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics* / Q000302:isolation & purification; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D035683:MicroRNAs
references.citation: Oncogene. 2011 Apr 28;30(17):1969-83
references.pmid: 21258404
references.citation: PLoS One. 2011;6(8):e23937
references.pmid: 21886843
references.citation: Nucleic Acids Res. 2012 Jan;40(Database issue):D109-14
references.pmid: 22080510
references.citation: J Orthop Res. 2012 Dec;30(12):1915-22
references.pmid: 22674437
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4402-7
references.pmid: 19246379
references.citation: Stroke. 2010 Dec;41(12):2894-900
references.pmid: 20966408
references.citation: Hepatology. 2008 Apr;47(4):1223-32
references.pmid: 18307259
references.citation: Nat Rev Cancer. 2013 Feb;13(2):123-35
references.pmid: 23344543
references.citation: FEBS Lett. 2011 Oct 3;585(19):3095-100
references.pmid: 21893058
references.citation: Cancer Epidemiol. 2012 Feb;36(1):e61-7
references.pmid: 22018950
references.citation: Science. 2005 Sep 2;309(5740):1577-81
references.pmid: 16141076
references.citation: Nature. 2007 Oct 18;449(7164):919-22
references.pmid: 17943132
references.citation: Hepatology. 2012 Nov;56(5):1631-40
references.pmid: 22610915
references.citation: Genome Res. 2003 Nov;13(11):2498-504
references.pmid: 14597658
references.citation: J Hepatol. 2009 Mar;50(3):453-60
references.pmid: 19144437
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6328-33
references.pmid: 20304794
references.citation: Mutat Res. 2011 Dec 1;717(1-2):85-90
references.pmid: 21402084
references.citation: Nucleic Acids Res. 2011 Jan;39(Database issue):D561-8
references.pmid: 21045058
references.citation: Hepatology. 2009 Apr;49(4):1098-112
references.pmid: 19173277
references.citation: Am J Gastroenterol. 2011 Sep;106(9):1663-9
references.pmid: 21606975
references.citation: Science. 2010 Jan 8;327(5962):198-201
references.pmid: 19965718
references.citation: Science. 1989 Apr 21;244(4902):362-4
references.pmid: 2496467
references.citation: FEBS Lett. 2012 May 7;586(9):1312-7
references.pmid: 22465663
references.citation: Hepatology. 2013 Sep;58(3):863-71
references.pmid: 23390075
references.citation: Nat Med. 2005 Jul;11(7):791-6
references.pmid: 15951748
references.citation: Mol Carcinog. 2011 Feb;50(2):136-42
references.pmid: 21229610
references.citation: Gut. 2011 May;60(5):688-94
references.pmid: 21068131
references.citation: Cancer Res. 2010 Dec 1;70(23):9798-807
references.pmid: 21098710
references.citation: J Hepatol. 2003 Mar;38(3):257-65
references.pmid: 12586290
references.citation: J Biol Chem. 2003 May 16;278(20):17775-84
references.pmid: 12621033
references.citation: PLoS One. 2011;6(10):e26971
references.pmid: 22066022