title: The Drosophila clotting system and its messages for mammals.
abstract: Drosophila has been increasingly used as a model to study hemolymph clotting. Proteomics and bioinformatics identified candidate clotting-factors, several of which were tested using genetics. Mutants and lines with reduced expression of clotting-factors show subtle effects after wounding, indicating that sealing wounds may rely on redundant mechanisms. More striking effects are observed after infection, in particular when a natural infection model involving entomopathogenic nematodes is used. When translated into mammalian models these results reveal that mammalian blood clots serve a similar immune function, thus providing a new example of the usefulness of studying invertebrate models.
authors.affiliation: Department of Molecular Biosciences, Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden. Electronic address: uli.theopold@su.se.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Theopold
authors.forename: Robert
authors.initials: R
authors.lastname: Krautz
authors.forename: Mitchell S
authors.initials: MS
authors.lastname: Dushay
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000235:genetics / Q000276:immunology; D001778:Blood Coagulation Disorders / Q000235:genetics / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology*; D019295:Computational Biology / Q000379:methods; D004195:Disease Models, Animal; D029721:Drosophila Proteins / Q000235:genetics / Q000276:immunology*; D004331:Drosophila melanogaster / Q000276:immunology*; D006458:Hemolymph / Q000276:immunology*; D006801:Humans; D008322:Mammals; D040901:Proteomics / Q000379:methods
chemical_list: D001798:Blood Proteins; D029721:Drosophila Proteins
keywords: APMAP; Hml; Ig; Innate immunity; Insect immunity; Phenoloxidase; Transglutaminase; adipocyte membrane associated protein; hemolectin; immunoglobulin