title: Periodontal regeneration following application of basic fibroblast growth factor-2 in combination with beta tricalcium phosphate in class III furcation defects in dogs.
abstract: The aim of this study was to investigate the effect of tunnel structured β-tricalcium phosphate (β-TCP) on the regenerative potential of basic fibroblast growth factor-2 (bFGF-2) in class III furcation defects in dogs. The furcations of 30 mandibular premolar teeth received: 1) 0.3% bFGF-2 solution in conjunction with β-TCP; 2) 0.3% bFGF-2 solution; and 3) no implant material (Control group). The dogs were sacrificed 8 weeks post-surgery, and healing was evaluated histologically. New bone formation was significantly greater in the bFGF-2/β-TCP group compared to the bFGF-2 solution and Control groups (p<0.01). New cementum formation in the bFGF-2/β-TCP and bFGF-2 solution groups was significantly greater than that in the Control group (p<0.01). These findings suggested that bFGF-2 alone enhances connective tissue attachment in a manner similar to the combination of bFGF-2 and β-TCP. Furthermore, this combination enhances bone formation up to the fornix in class III furcation defects.
authors.affiliation: Department of Oral Rehabilitation, Division of Oral Functional Science, Hokkaido University Graduate School of Dental Medicine, Kita-ku, Sapporo, Japan. saito@den.hokudai.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Saito
authors.forename: Emiko
authors.initials: E
authors.lastname: Saito
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kuboki
authors.forename: Mariko
authors.initials: M
authors.lastname: Kimura
authors.forename: Toshinori
authors.initials: T
authors.lastname: Nakajima
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yuge
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kato
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Honma
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Noboru
authors.initials: N
authors.lastname: Ohata
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001641:Bicuspid / Q000601:surgery; D001672:Biocompatible Materials / Q000627:therapeutic use*; D015415:Biomarkers / Q000032:analysis; D018786:Bone Substitutes / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000627:therapeutic use*; D025021:Cementogenesis / Q000502:physiology; D003094:Collagen; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D017823:Furcation Defects / Q000145:classification / Q000601:surgery*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods; D007527:Isoenzymes / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology; D010513:Periodontal Ligament / Q000187:drug effects*; D011897:Random Allocation; D012038:Regeneration / Q000187:drug effects; D016745:Root Planing / Q000379:methods; D012391:Root Resorption / Q000209:etiology; D000071681:Tartrate-Resistant Acid Phosphatase; D020254:Tooth Ankylosis / Q000209:etiology
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D018786:Bone Substitutes; D002130:Calcium Phosphates; D007527:Isoenzymes; C485817:beta-tricalcium phosphate; D016222:Fibroblast Growth Factor 2; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase