title: The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii.
abstract: An extended outbreak of mycobacterial surgical infections occurred in Brazil during 2004-2008. Most infections were caused by a single strain of Mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpoB sequevar and two highly similar pulsed-field gel electrophoresis (PFGE) patterns differentiated by the presence of a ∼50 kb band. The nature of this band was investigated.
  
  
  
  Genomic sequencing of the prototype outbreak isolate INCQS 00594 using the SOLiD platform demonstrated the presence of a 56,267-bp [corrected] circular plasmid, designated pMAB01. Identity matrices, genetic distances and phylogeny analyses indicated that pMAB01 belongs to the broad-host-range plasmid subgroup IncP-1β and is highly related to BRA100, pJP4, pAKD33 and pB10. The presence of pMAB01-derived sequences in 41 M. abscessus subsp. bolletii isolates was evaluated using PCR, PFGE and Southern blot hybridization. Sixteen of the 41 isolates showed the presence of the plasmid. The plasmid was visualized as a ∼50-kb band using PFGE and Southern blot hybridization in 12 isolates. The remaining 25 isolates did not exhibit any evidence of this plasmid. The plasmid was successfully transferred to Escherichia coli by conjugation and transformation. Lateral transfer of pMAB01 to the high efficient plasmid transformation strain Mycobacterium smegmatis mc(2)155 could not be demonstrated.
  
  
  
  The occurrence of a broad-host-range IncP-1β plasmid in mycobacteria is reported for the first time. Thus, genetic exchange could result in the emergence of specific strains that might be better adapted to cause human disease.
authors.affiliation: Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia da Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil. sylvia.leao@unifesp.br
authors.forename: Sylvia Cardoso
authors.initials: SC
authors.lastname: Leão
authors.forename: Cristianne Kayoko
authors.initials: CK
authors.lastname: Matsumoto
authors.forename: Adriana
authors.initials: A
authors.lastname: Carneiro
authors.forename: Rommel Thiago
authors.initials: RT
authors.lastname: Ramos
authors.forename: Christiane Lourenço
authors.initials: CL
authors.lastname: Nogueira
authors.forename: James Daltro
authors.initials: JD
authors.lastname: Lima
authors.forename: Karla Valéria
authors.initials: KV
authors.lastname: Lima
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Lopes
authors.forename: Horacio
authors.initials: H
authors.lastname: Schneider
authors.forename: Vasco Ariston
authors.initials: VA
authors.lastname: Azevedo
authors.forename: Artur
authors.initials: A
authors.lastname: da Costa da Silva
mesh_terms: D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction
references.citation: Appl Environ Microbiol. 2006 Nov;72(11):7253-9
references.pmid: 16980416
references.citation: Genome Res. 2008 May;18(5):729-41
references.pmid: 18403782
references.citation: BMC Res Notes. 2011 Apr 18;4:130
references.pmid: 21501521
references.citation: Nucleic Acids Res. 1988 Feb 11;16(3):925-39
references.pmid: 3344223
references.citation: Genome Biol. 2010;11(4):R41
references.pmid: 20388197
references.citation: Antimicrob Agents Chemother. 1991 May;35(5):819-23
references.pmid: 1854163
references.citation: Microbiology (Reading). 2005 Mar;151(Pt 3):683-692
references.pmid: 15758215
references.citation: Plasmid. 2012 May;67(3):252-8
references.pmid: 22107909
references.citation: Stand Genomic Sci. 2011 Oct 15;5(1):144-53
references.pmid: 22180818
references.citation: Plasmid. 2002 Jul;48(1):1-12
references.pmid: 12206751
references.citation: J Clin Microbiol. 2009 Sep;47(9):2691-8
references.pmid: 19571015
references.citation: Mol Genet Genomics. 2003 Feb;268(5):570-84
references.pmid: 12589432
references.citation: Mem Inst Oswaldo Cruz. 2012 Dec;107(8):969-77
references.pmid: 23295745
references.citation: Nat Genet. 2003 May;34(1):80-4
references.pmid: 12669068
references.citation: Syst Biol. 2004 Oct;53(5):793-808
references.pmid: 15545256
references.citation: Microbiology (Reading). 1998 Jul;144 ( Pt 7):1981-1988
references.pmid: 9695931
references.citation: Proc Natl Acad Sci U S A. 1987 Jun;84(12):4054-7
references.pmid: 3295875
references.citation: FEMS Microbiol Lett. 1994 Feb 1;116(1):19-24
references.pmid: 7907567
references.citation: Nat Commun. 2011;2:268
references.pmid: 21468020
references.citation: Plasmid. 2005 Sep;54(2):135-48
references.pmid: 16122561
references.citation: J Clin Microbiol. 2011 Jan;49(1):62-8
references.pmid: 21084521
references.citation: Appl Environ Microbiol. 2011 Nov;77(22):7975-83
references.pmid: 21948829
references.citation: J Mol Biol. 1994 Jun 24;239(5):623-63
references.pmid: 8014987
references.citation: Microbiology (Reading). 2007 Feb;153(Pt 2):452-463
references.pmid: 17259616
references.citation: Microbiology (Reading). 2003 Nov;149(Pt 11):3139-3153
references.pmid: 14600226
references.citation: Microbiology (Reading). 2006 Sep;152(Pt 9):2689-2701
references.pmid: 16946264
references.citation: Microbiology (Reading). 2004 Nov;150(Pt 11):3591-3599
references.pmid: 15528648
references.citation: Plasmid. 2007 Jul;58(1):31-43
references.pmid: 17306874
references.citation: J Bacteriol. 1984 Feb;157(2):669-72
references.pmid: 6693354
references.citation: J Bacteriol. 1997 Apr;179(8):2753-6
references.pmid: 9098076
references.citation: PLoS One. 2012;7(6):e39107
references.pmid: 22723939
references.citation: Plasmid. 2006 Jul;56(1):11-23
references.pmid: 16445980
references.citation: Microbiology (Reading). 2006 Dec;152(Pt 12):3477-3484
references.pmid: 17159199
references.citation: FEMS Microbiol Rev. 2007 Jul;31(4):449-77
references.pmid: 17553065
references.citation: Braz J Infect Dis. 2011 Sep-Oct;15(5):436-41
references.pmid: 22230849
references.citation: J Clin Microbiol. 2009 Jul;47(7):2149-55
references.pmid: 19403765
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Jpn J Microbiol. 1971 Jul;15(4):359-66
references.pmid: 5315708
references.citation: Microb Drug Resist. 2012 Feb;18(1):1-6
references.pmid: 21711149
references.citation: BMC Genomics. 2008 Feb 08;9:75
references.pmid: 18261238
references.citation: Bioinformatics. 2003 Aug 12;19(12):1572-4
references.pmid: 12912839
references.citation: PLoS One. 2012;7(1):e29884
references.pmid: 22235347
references.citation: Microbes Infect. 2008 Nov-Dec;10(14-15):1552-7
references.pmid: 18950729
references.citation: Gene. 1988 Nov 30;71(2):315-21
references.pmid: 3224826
references.citation: J Clin Microbiol. 2011 Feb;49(2):491-9
references.pmid: 21106786
references.citation: J Clin Microbiol. 2008 Mar;46(3):850-5
references.pmid: 18174307
references.citation: J Bacteriol. 2004 Nov;186(21):7161-74
references.pmid: 15489427
references.citation: Future Microbiol. 2010 Jun;5(6):971-80
references.pmid: 20521940
references.citation: J Mol Biol. 1998 Oct 9;282(5):969-90
references.pmid: 9753548
references.citation: PLoS One. 2009 Jun 19;4(6):e5660
references.pmid: 19543527
references.citation: Mol Microbiol. 1990 Nov;4(11):1911-9
references.pmid: 2082148
references.citation: J Clin Microbiol. 2010 Jun;48(6):2257-9
references.pmid: 20392924
references.citation: Acta Biochim Pol. 2003;50(2):425-53
references.pmid: 12833168
references.citation: Microbiology (Reading). 2012 Aug;158(Pt 8):2060-2072
references.pmid: 22653947
references.citation: Microbiology (Reading). 2011 Jun;157(Pt 6):1714-1725
references.pmid: 21393370
references.citation: Environ Microbiol. 2004 Jul;6(7):655-68
references.pmid: 15186344
references.citation: Bioinformatics. 2000 Oct;16(10):944-5
references.pmid: 11120685
references.citation: Acta Cir Bras. 2010 Oct;25(5):455-9
references.pmid: 20877958
references.citation: Mol Gen Genet. 2000 Apr;263(3):471-82
references.pmid: 10821181
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: Minim Invasive Surg. 2011;2011:724635
references.pmid: 22110914