title: The 1.58 Å resolution structure of the DNA-binding domain of bacteriophage SF6 small terminase provides new hints on DNA binding.
abstract: DNA packaging in tailed bacteriophages and in evolutionarily related herpesviruses is controlled by a viral-encoded terminase. As in a number of other phages, in the Bacillus subtilis bacteriophages SF6 and SPP1 the terminase complex consists of two proteins: G1P and G2P. The crystal structure of the N-terminal DNA-binding domain of the bacteriophage SF6 small terminase subunit G1P is reported. Structural comparison with other DNA-binding proteins allows a general model for the interaction of G1P with the packaging-initiation site to be proposed.
authors.affiliation: Laboratory of Bioorganic Chemistry and Crystallography, Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, 39100 Bolzano, Italy. stefano.benini@unibz.it
authors.forename: Stefano
authors.initials: S
authors.lastname: Benini
authors.forename: Maria
authors.initials: M
authors.lastname: Chechik
authors.forename: Miguel
authors.initials: M
authors.lastname: Ortiz Lombardía
authors.forename: Sigrun
authors.initials: S
authors.lastname: Polier
authors.forename: Andrew
authors.initials: A
authors.lastname: Leech
authors.forename: Mikhail B
authors.initials: MB
authors.lastname: Shevtsov
authors.forename: Juan C
authors.initials: JC
authors.lastname: Alonso
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D017103:Bacillus Phages / Q000201:enzymology*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D054730:Protein Interaction Domains and Motifs; D016415:Sequence Alignment
chemical_list: D004247:DNA; D004706:Endodeoxyribonucleases; D000251:Adenosine Triphosphatases
keywords: DNA-binding domains; G1P; SF6; bacteriophages; terminases; viruses
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