title: 1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice.
abstract: We investigated the role of 1-deoxynojirimycin (DNJ) on glucose absorption and metabolism in normal and diabetic mice. Oral and intravenous glucose tolerance tests and labeled (13)C6-glucose uptake assays suggested that DNJ inhibited intestinal glucose absorption in intestine. We also showed that DNJ down-regulated intestinal SGLT1, Na(+)/K(+)-ATP and GLUT2 mRNA and protein expression. Pretreatment with DNJ (50 mg/kg) increased the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreased the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase). Assays of protein expression in hepatic cells and in vitro tests with purified enzymes indicated that the increased activity of glucose glycolysis enzymes was resulted from the relative increase in protein expression, rather than from direct enzyme activation. These results suggest that DNJ inhibits intestinal glucose absorption and accelerates hepatic glucose metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes.
authors.affiliation: Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: You-Gui
authors.initials: YG
authors.lastname: Li
authors.forename: Dong-Feng
authors.initials: DF
authors.lastname: Ji
authors.forename: Shi
authors.initials: S
authors.lastname: Zhong
authors.forename: Tian-Bao
authors.initials: TB
authors.lastname: Lin
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Lv
authors.forename: Gui-Yan
authors.initials: GY
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D017485:1-Deoxynojirimycin / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000096:biosynthesis / Q000187:drug effects*; D051273:Glucose Transporter Type 2 / Q000096:biosynthesis / Q000235:genetics; D006019:Glycolysis / Q000187:drug effects; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000096:biosynthesis; D051269:Sodium-Glucose Transporter 1 / Q000096:biosynthesis / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis / Q000235:genetics; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D004791:Enzyme Inhibitors; D051246:Glucose Transport Proteins, Facilitative; D051273:Glucose Transporter Type 2; D012333:RNA, Messenger; C495682:Slc2a2 protein, mouse; C495675:Slc5a1 protein, mouse; D051269:Sodium-Glucose Transporter 1; D017485:1-Deoxynojirimycin; D013311:Streptozocin; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose
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