title: An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce.
abstract: We have generated an ultra-high-density genetic map for lettuce, an economically important member of the Compositae, consisting of 12,842 unigenes (13,943 markers) mapped in 3696 genetic bins distributed over nine chromosomal linkage groups. Genomic DNA was hybridized to a custom Affymetrix oligonucleotide array containing 6.4 million features representing 35,628 unigenes of Lactuca spp. Segregation of single-position polymorphisms was analyzed using 213 F7:8recombinant inbred lines that had been generated by crossing cultivated Lactuca sativa cv. Salinas and L. serriola acc. US96UC23, the wild progenitor species of L. sativa The high level of replication of each allele in the recombinant inbred lines was exploited to identify single-position polymorphisms that were assigned to parental haplotypes. Marker information has been made available using GBrowse to facilitate access to the map. This map has been anchored to the previously published integrated map of lettuce providing candidate genes for multiple phenotypes. The high density of markers achieved in this ultradense map allowed syntenic studies between lettuce and Vitis vinifera as well as other plant species.
authors.affiliation: The Genome Center, University of California, Davis, California 95616.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Truco
authors.affiliation: Seed Biotechnology Center, University of California, Davis, California 95616.
authors.forename: Hamid
authors.initials: H
authors.lastname: Ashrafi
authors.affiliation: The Genome Center, University of California, Davis, California 95616.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kozik
authors.affiliation: The Genome Center, University of California, Davis, California 95616.
authors.forename: Hans
authors.initials: H
authors.lastname: van Leeuwen
authors.affiliation: Department of Plant Biology, University of Georgia, Athens, Georgia 30602.
authors.forename: John
authors.initials: J
authors.lastname: Bowers
authors.affiliation: The Genome Center, University of California, Davis, California 95616.
authors.forename: Sebastian Reyes Chin
authors.initials: SR
authors.lastname: Wo
authors.affiliation: Seed Biotechnology Center, University of California, Davis, California 95616.
authors.forename: Kevin
authors.initials: K
authors.lastname: Stoffel
authors.affiliation: The Genome Center, University of California, Davis, California 95616.
authors.forename: Huaqin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Seed Biotechnology Center, University of California, Davis, California 95616.
authors.forename: Theresa
authors.initials: T
authors.lastname: Hill
authors.affiliation: Seed Biotechnology Center, University of California, Davis, California 95616.|Department of Plant Sciences, University of California, Davis, California 95616.
authors.forename: Allen
authors.initials: A
authors.lastname: Van Deynze
authors.affiliation: The Genome Center, University of California, Davis, California 95616 rwmichelmore@ucdavis.edu.|Department of Plant Sciences, University of California, Davis, California 95616.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Michelmore
mesh_terms: D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D018545:Lactuca / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype
chemical_list: D005819:Genetic Markers
keywords: Lactuca sativa; linkage analysis; microarray; recombination
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