title: Potential roles of CCR5(+) CCR6(+) dendritic cells induced by nasal ovalbumin plus Flt3 ligand expressing adenovirus for mucosal IgA responses.
abstract: We assessed the role of CCR5(+)/CCR6(+)/CD11b(+)/CD11c(+) dendritic cells (DCs) for induction of ovalbumin (OVA)-specific antibody (Ab) responses following mucosal immunization. Mice given nasal OVA plus an adenovirus expressing Flt3 ligand (Ad-FL) showed early expansion of CCR5(+)/CCR6(+)/CD11b(+)/CD11c(+) DCs in nasopharyngeal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLNs). Subsequently, this DC subset became resident in submandibular glands (SMGs) and nasal passages (NPs) in response to high levels of CCR-ligands produced in these tissues. CD11b(+)/CD11c(+) DCs were markedly decreased in both CCR5(-/-) and CCR6(-/-) mice. Chimera mice reconstituted with bone marrow cells from CD11c-diphtheria toxin receptor (CD11c-DTR) and CCR5(-/-) or CD11c-DTR and CCR6(-/-) mice given nasal OVA plus Ad-FL had elevated plasma IgG, but reduced IgA as well as low anti-OVA secretory IgA (SIgA )Ab responses in saliva and nasal washes. These results suggest that CCR5(+)CCR6(+) DCs play an important role in the induction of Ag-specific SIgA Ab responses.
authors.affiliation: Departments of Pediatric Dentistry and Microbiology, The Immunobiology Vaccine Center, The Institute of Oral Health Research, The University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Fukuyama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tokuhara
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sekine
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Aso
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kataoka
authors.forename: Julia
authors.initials: J
authors.lastname: Davydova
authors.forename: Masato
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Rebekah S
authors.initials: RS
authors.lastname: Gilbert
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tokuhara
authors.forename: Keiko
authors.initials: K
authors.lastname: Fujihashi
authors.forename: Jun
authors.initials: J
authors.lastname: Kunisawa
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Yuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
authors.forename: Jerry R
authors.initials: JR
authors.lastname: McGhee
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Fujihashi
mesh_terms: D000256:Adenoviridae; D000281:Administration, Intranasal; D000818:Animals; D003713:Dendritic Cells / Q000378:metabolism*; D007070:Immunoglobulin A / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009092:Mucous Membrane / Q000187:drug effects / Q000276:immunology*; D010047:Ovalbumin / Q000494:pharmacology*; D019713:Receptors, CCR5 / Q000378:metabolism*; D054399:Receptors, CCR6 / Q000378:metabolism*
chemical_list: D007070:Immunoglobulin A; D008565:Membrane Proteins; D019713:Receptors, CCR5; D054399:Receptors, CCR6; C084881:flt3 ligand protein; D010047:Ovalbumin
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