title: Antigenotoxic properties of chlorophyll b against cisplatin-induced DNA damage and its relationship with distribution of platinum and magnesium in vivo.
abstract: The chemotherapeutic agent cisplatin (cDDP) is widely used to treat a variety of solid and hematological tumors. However, cDDP exerts severe side effects, such as nephrotoxicity, neurotoxicity, and bone-marrow suppression. The use of some dietary compounds to protect organs that are not targets in association with chemotherapy has been encouraged. This study evaluated the protective effects of chlorophyll b (CLb) on DNA damage induced by cDDP by use of single-cell gel electrophoresis (SCGE) assays. Further, this investigation also determined platinum (Pt) and magnesium (Mg) bioaccumulation in mice tissues after treatment with CLb alone and/or in association of cDDP (simultaneous treatment) by inductively coupled plasma-mass spectroscopy (ICP-MS). All parameters were studied in peripheral blood cells (PBC), kidneys, and liver of mice after administration of CLb (0.2 or 0.5 mg/kg of body weight [b.w.]), cDDP (6 mg/kg b.w.), and the combination CLb 0.2 plus cDDP or CLb 0.5 plus cDDP. Pt accumulation in liver and kidneys was higher than that found in PBC, while DNA damage was higher in kidneys and liver than in PBC. Further, treatment with CLb alone did not induce DNA damage. Evidence indicates that genotoxic effects produced by cDDP may not be related to Pt accumulation and distribution. In combined treatments, CLb decreased DNA damage in tissues, but the PT contents did not change and these treatments also showed that CLb may be an important source of Mg. Thus, our results indicate that consumption of CLb-rich foods may diminish the adverse health effects induced by cDDP exposure.
authors.affiliation: Department of Clinical, Toxicologic, and Bromatological Analysis, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Juliana Mara
authors.initials: JM
authors.lastname: Serpeloni
authors.forename: Bruno Lemos
authors.initials: BL
authors.lastname: Batista
authors.forename: José Pedro Friedmann
authors.initials: JP
authors.lastname: Angeli
authors.forename: Gustavo Rafael Mazzaron
authors.initials: GR
authors.lastname: Barcelos
authors.forename: Maria de Lourdes Pires
authors.initials: Mde L
authors.lastname: Bianchi
authors.forename: Fernando
authors.initials: F
authors.lastname: Barbosa
authors.forename: Lusânia Maria Greggi
authors.initials: LM
authors.lastname: Antunes
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000633:toxicity*; D002734:Chlorophyll / Q000494:pharmacology*; D002945:Cisplatin / Q000493:pharmacokinetics / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D017616:Magnesium Compounds / Q000378:metabolism; D008297:Male; D051379:Mice; D017671:Platinum Compounds / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D000970:Antineoplastic Agents; D017616:Magnesium Compounds; D017671:Platinum Compounds; D002734:Chlorophyll; C037184:chlorophyll b; D002945:Cisplatin