title: "Salt tolerant" anion exchange chromatography for direct capture of an acidic protein from CHO cell culture.
abstract: The present study describes the use of the new HyperCel STAR AX "salt tolerant" anion exchange sorbent for the capture from Chinese Hamster Ovary (CHO) cell culture supernatant (CCS) of an acidic model protein (α-amylase). HyperCel STAR AX sorbent and other conventional anion exchangers were evaluated to purify biologically-active enzyme. Salt tolerance of the sorbent allowed reaching 5-fold higher dynamic binding capacity than conventional anion exchange during capture of the enzyme from neat (undiluted) CCS. After optimization of operating conditions, HyperCel STAR AX turned out to be the only sorbent allowing efficient protein capture directly from both neat and diluted CCS with consistent and satisfying purity, yield and productivity. Therefore implementation of the salt tolerant sorbent in industrial purification processes should allow avoiding time and cost consuming steps such as dilution or UF/DF that exclusively aim at establishing suitable conditions for ion exchange step without bringing any added value to the purification process performance. Altogether this study highlights the flexibility and cost-reduction potential brought in process design by the HyperCel STAR AX salt tolerant sorbent.
authors.affiliation: Pall Life Sciences, 48 avenue des Genottes, 95800 Cergy Saint Christophe, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Champagne
authors.forename: Guillaume
authors.initials: G
authors.lastname: Balluet
authors.forename: René
authors.initials: R
authors.lastname: Gantier
authors.forename: Magali
authors.initials: M
authors.lastname: Toueille
mesh_terms: D000327:Adsorption; D000818:Animals; D000838:Anions / Q000737:chemistry; D016466:CHO Cells; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D004795:Enzyme Stability; D012492:Salts / Q000737:chemistry; D000516:alpha-Amylases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000838:Anions; D012492:Salts; D000516:alpha-Amylases