title: Patagonian red wines: selection of Lactobacillus plantarum isolates as potential starter cultures for malolactic fermentation.
abstract: The aim of this study was to evaluate fifty-three Lactobacillus plantarum isolates obtained from a Patagonian red wine, molecularly identified and typified using RAPD analysis, in order to select starter cultures for malolactic fermentation (MLF). The results obtained suggest a considerable genetic diversity, taking into account that all L. plantarum isolates were obtained from one cellar and one vintage. Based on the capacity to tolerate a concentration of 14 % ethanol in MRS broth for 2 days, eight isolates were selected for the subsequent analysis. The incidence of various wine stress factors (ethanol, acid pH, lysozyme and sulfur dioxide) on isolates growth was studied. Besides, glucosidase and tannase activities were evaluated, and the presence of genes involved in the synthesis of biogenic amines was examined by PCR. A previously characterized indigenous Oenococcus oeni strain was included with comparative purposes. Differences in technologically relevant characteristics were observed among the eight L. plantarum selected isolates, revealing an isolate-dependent behavior. Detectable glucosidase and tannase activities were found in all isolates. The presence of genes encoding histidine and tyrosine descarboxylases and putrescine carbamoyltransferase was not detected. The ability of L. plantarum isolates to grow and consume L-malic acid in simulated laboratory-scale vinifications revealed that two of them could be considered as possible MLF starter cultures for Patagonian red wines. These isolates will be subjected to further analysis, for a final winery technological characterization.
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencia y Tecnología, Instituto de Microbiología Básica y Aplicada-IMBA, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina. bbferrada@unq.edu.ar
authors.forename: Bárbara Mercedes
authors.initials: BM
authors.lastname: Bravo-Ferrada
authors.forename: Axel
authors.initials: A
authors.lastname: Hollmann
authors.forename: Lucrecia
authors.initials: L
authors.lastname: Delfederico
authors.forename: Danay
authors.initials: D
authors.lastname: Valdés La Hens
authors.forename: Adriana
authors.initials: A
authors.lastname: Caballero
authors.forename: Liliana
authors.initials: L
authors.lastname: Semorile
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D005959:Glucosidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D008293:Malates / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D014920:Wine / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D008293:Malates; D019344:Lactic Acid; C030298:malic acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase; D005959:Glucosidases
references.citation: Curr Microbiol. 2011 Jun;62(6):1682-8
references.pmid: 21404095
references.citation: J Appl Microbiol. 2000 Apr;88(4):584-93
references.pmid: 10792516
references.citation: Lett Appl Microbiol. 1995 Dec;21(6):376-9
references.pmid: 8554764
references.citation: Nucleic Acids Res. 1989 Oct 11;17(19):7843-53
references.pmid: 2798131
references.citation: Int J Food Microbiol. 2003 Jul 15;84(1):117-23
references.pmid: 12781962
references.citation: J Appl Microbiol. 2006 Apr;100(4):712-27
references.pmid: 16553726
references.citation: Appl Environ Microbiol. 1989 Aug;55(8):2010-2016
references.pmid: 16347992
references.citation: Int J Food Microbiol. 2004 Nov 1;96(2):199-204
references.pmid: 15364474
references.citation: J Agric Food Chem. 2004 Apr 7;52(7):1861-6
references.pmid: 15053521
references.citation: FEMS Microbiol Lett. 2003 Dec 5;229(1):65-71
references.pmid: 14659544
references.citation: Lett Appl Microbiol. 2010 Mar;50(3):327-34
references.pmid: 20408255
references.citation: J Appl Microbiol. 2002;92(4):589-601
references.pmid: 11966898
references.citation: Food Microbiol. 2007 Aug;24(5):482-91
references.pmid: 17367681
references.citation: Int J Food Microbiol. 2010 Dec 15;144(2):270-9
references.pmid: 21035223
references.citation: J Microbiol Biotechnol. 2011 Dec;21(12):1280-6
references.pmid: 22210614
references.citation: Int J Food Microbiol. 2004 Nov 15;96(3):235-52
references.pmid: 15454314
references.citation: FEMS Microbiol Lett. 2004 Jan 15;230(1):53-61
references.pmid: 14734166
references.citation: J Ind Microbiol Biotechnol. 2007 Aug;34(8):539-46
references.pmid: 17576609
references.citation: Antonie Van Leeuwenhoek. 1999 Jul-Nov;76(1-4):317-31
references.pmid: 10532386
references.citation: Int J Food Microbiol. 2004 Feb 15;91(1):19-29
references.pmid: 14967557
references.citation: FEMS Microbiol Lett. 2002 May 21;211(1):85-9
references.pmid: 12052555
references.citation: Adv Appl Microbiol. 1997;44:215-60
references.pmid: 9311108
references.citation: Biochim Biophys Acta. 2005 Nov 30;1717(2):118-24
references.pmid: 16271350
references.citation: Int J Food Microbiol. 2010 Feb 28;137(2-3):230-5
references.pmid: 20051306
references.citation: Microb Ecol. 2012 Jan;63(1):12-9
references.pmid: 21779812
references.citation: J Microbiol Biotechnol. 2012 Apr;22(4):516-25
references.pmid: 22534299
references.citation: Food Microbiol. 2008 Oct;25(7):942-8
references.pmid: 18721686
references.citation: J Appl Microbiol. 2010 Jan;108(1):285-98
references.pmid: 19614854
references.citation: Food Microbiol. 2008 Dec;25(8):983-91
references.pmid: 18954734
references.citation: Appl Environ Microbiol. 1980 Apr;39(4):926-7
references.pmid: 16345559
references.citation: Int Microbiol. 2010 Dec;13(4):169-77
references.pmid: 21404211
references.citation: J Appl Bacteriol. 1995 Mar;78(3):316-26
references.pmid: 7730207
references.citation: Appl Environ Microbiol. 1993 Apr;59(4):1251-2
references.pmid: 16348918
references.citation: Syst Appl Microbiol. 2003 Sep;26(3):412-22
references.pmid: 14529184
references.citation: Theor Appl Genet. 1993 Sep;86(8):991-1000
references.pmid: 24194008
references.citation: J Dairy Res. 2006 Feb;73(1):20-7
references.pmid: 16433957
references.citation: J Microbiol Methods. 2007 May;69(2):387-90
references.pmid: 17303271