title: Oxidation of Microcystis aeruginosa and Anabaena flos-aquae by ozone: impacts on cell integrity and chlorination by-product formation.
abstract: Pre-ozonation of cyanobacterial (CB) cells in raw water and inter-ozonation of settled water can cause CB cell damage. However, there is limited information about the level of lysis or changes in cell properties after ozonation, release of intracellular compounds and their contribution to the formation of disinfection by-products (DBPs). This study aims to: (1) assess the extent of the pre-ozonation effects on CB cell properties; (2) determine the CT (ozone concentration × detention time) values required for complete loss of cell viability; and (3) study the DBPs formation associated with the pre-ozonation of cyanobacterial cells in laboratorial suspensions. To these ends, both Microcystis aeruginosa and Anabaena flos-aquae suspensions were prepared at concentrations of 250,000 cells mL(-1) and 1,500,000 cells mL(-1) and were subjected to ozone dosages of 0.5, 2.0 and 4.0 mg L(-1) at pH 6 and pH 8. A quick and complete loss of viability was achieved for both CB species after exposure (CT) to ozone of <0.2 mg min L(-1), although no significant decrease in total cell numbers was observed. Maximum dissolved organic carbon (DOC) releases of 0.96 mg L(-1) and 1.63 mg L(-1) were measured after ozonation of 250,000 cells mL(-1) of M. aeruginosa and A. flos-aquae, respectively. DOC release was found to be pH and ozone dose dependent. Ozonation of CB cells increased formation of trihalomethanes (THM) and haloacetic acids (HAA), mainly for suspensions of A. flos-aquae at pH 8 (by 174% and 65% for THM and HAA respectively). Utilities considering using ozone for oxidising CB cells should weigh out the benefit of CB control with the potential increased formation of chlorinated DBPs.
authors.affiliation: École Polytechnique de Montreal, Civil, Mineral and Mining Engineering Department, P.O. Box 6079, Station Centre-ville, Montreal, Quebec, Canada. lucila_coral@yahoo.com.br
authors.forename: Lucila A
authors.initials: LA
authors.lastname: Coral
authors.forename: Arash
authors.initials: A
authors.lastname: Zamyadi
authors.forename: Benoit
authors.initials: B
authors.lastname: Barbeau
authors.forename: Fatima J
authors.initials: FJ
authors.lastname: Bassetti
authors.forename: Flávio R
authors.initials: FR
authors.lastname: Lapolli
authors.forename: Michèle
authors.initials: M
authors.lastname: Prévost
mesh_terms: D002244:Carbon / Q000032:analysis; D004203:Disinfection; D046869:Dolichospermum flos-aquae / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D054879:Halogenation / Q000187:drug effects*; D007700:Kinetics; D046931:Microcystis / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D010084:Oxidation-Reduction / Q000187:drug effects; D010126:Ozone / Q000494:pharmacology*; D022882:Trihalomethanes / Q000032:analysis
chemical_list: D022882:Trihalomethanes; D010126:Ozone; D002244:Carbon