title: Use of gel permeation chromatography for clean-up in the analysis of coccidiostats in eggs by liquid chromatography-tandem mass spectrometry.
abstract: An analytical method for determination and confirmation of nine coccidiostatics in eggs is reported. Ethyl acetate is used as extraction solvent, with satisfactory results, and simple automated clean-up is based on gel-permeation chromatography (GPC) . The target compounds are then analysed by liquid chromatography-electrospray ionization-tandem mass spectrometry. The method was validated in-house in accordance with Commission Decision 2002/657/EC. Trueness and precision were determined at four concentrations, and the mean errors obtained were <10 %, with relative standard deviations ranging from 3 to 18 %. For three non-authorized coccidiostatics (clopidol, ethopabate, and ronizadole), decision limit and detection capability were in the ranges 0.12-0.16 and 0.18-0.23 μg kg(-1), respectively. The results obtained prove the suitability of this new analytical method for routine monitoring of these substances in eggs.
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Chico
authors.forename: A
authors.initials: A
authors.lastname: Rúbies
authors.forename: F
authors.initials: F
authors.lastname: Centrich
authors.forename: R
authors.initials: R
authors.lastname: Companyó
authors.forename: M D
authors.initials: MD
authors.lastname: Prat
authors.forename: M
authors.initials: M
authors.lastname: Granados
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003049:Coccidiostats