title: Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins.
abstract: A novel strategy for preparation of a boronate affinity hybrid monolith was developed using a Cu(I)-catalyzed 1,3-dipolar azide-alkyne cycloaddition (CuAAC) reaction of an alkyne-boronate ligand with an azide-functionalized monolithic intermediate. An azide-functionalized hybrid monolith was first synthesized via a single-step procedure to provide reactive sites for click chemistry; then the alkyne-boronate ligands were covalently immobilized on the azide-functionalized hybrid monolith via an in-column CuAAC reaction to form a boronate affinity hybrid monolith under mild conditions. The boronate affinity monolith was characterized and evaluated by means of elemental analysis, Fourier transform infrared spectroscopy, and scanning electron microscopy. The boronate affinity hybrid monolith exhibited excellent specificity toward nucleosides and glycoproteins, which were chosen as test cis-diol-containing compounds under neutral conditions. The binding capacity of the monolith for the glycoprotein ovalbumin was 2.36 mg · g(-1) at pH 7.0. The practicability of the boronate affinity hybrid monolithic material was demonstrated by specific capture of the glycoproteins ovalbumin and ovotransferrin from an egg sample.
authors.affiliation: State Key Laboratory of Medical Chemical Biology, Department of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Mao
authors.forename: X W
authors.initials: XW
authors.lastname: He
authors.forename: L X
authors.initials: LX
authors.lastname: Chen
authors.forename: Y K
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry*; D015394:Molecular Structure; D011485:Protein Binding
chemical_list: D001897:Boronic Acids; D006023:Glycoproteins