title: Flavobacterium marinum sp. nov., isolated from seawater.
abstract: A Gram-staining-negative, strictly aerobic, non-gliding, rod-shaped bacterial strain, designated SW105(T), was isolated from a seawater sample collected from the Indian Ocean. The strain produced flexirubin-type pigments and grew at 15-45 °C (optimum, 35 °C), at pH 5.5-8.5 (optimum, pH 7.0-7.5) and in the presence of 0-5.0 % (w/v) NaCl (optimum, 1.0-1.5 %). The predominant cellular fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 1ω9c and iso-C17 : 0 3-OH. The major menaquinone was menaquinone 6 (MK-6) and the major polar lipids were phosphatidylethanolamine and two unidentified aminophospholipids. The genomic DNA G+C content of strain SW105(T) was 36.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate was related to members of the genus Flavobacterium, showing the highest similarity to Flavobacterium ummariense DS-12(T) and Flavobacterium ceti CCUG 52969(T) (94.3 and 93.0 % sequence similarity, respectively). On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain SW105(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium marinum sp. nov. is proposed. The type strain is SW105(T) ( = CGMCC 1.10825(T) = JCM 18132(T)).
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dai
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019156:Indian Ocean; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D011090:Polyenes / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D011090:Polyenes; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C017313:flexirubins; C042455:menaquinone 6