title: The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection.
abstract: Carbohydrates of pathogen antigens have been disrupted by periodate oxidation, in order to reduce nonspecific bindings and improve serodiagnosis of parasite infections. In the present study, the enzyme-linked immunosorbent assay (ELISA) was carried out with filariform larvae antigen treated, or not treated, with sodium metaperiodate. Groups of sera from patients with Strongyloides stercoralis infection, with other intestinal parasites and a normal control, were used. The oxidation of Strongyloides stercoralis glycosylated epitopes reduced the seroreactivity of sera from patients with S. stercoralis infection as demonstrated by ELISA, with a decrease in sera optical densities. The number of cross-reactions of IgG and IgE-ELISAs increased by 12% and 16%, respectively, after antigen treatment with metaperiodate. This was more often observed in patients infected with Schistosoma mansoni and hookworm. Moreover, the IgG depletion from sera tested by IgE-ELISA led to the detection of previous false-negative samples from S. stercoralis-infected patients.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal da Bahia, 40170-115, Salvador, Bahia, Brazil.
authors.forename: Elizabete de Jesus
authors.initials: Ede J
authors.lastname: Inês
authors.forename: Mônica Lopes Sampaio
authors.initials: ML
authors.lastname: Silva
authors.forename: Joelma Nascimento
authors.initials: JN
authors.lastname: Souza
authors.forename: Márcia Cristina Aquino
authors.initials: MC
authors.lastname: Teixeira
authors.forename: Neci Matos
authors.initials: NM
authors.lastname: Soares
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000097:blood*; D005243:Feces / Q000469:parasitology; D006031:Glycosylation; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007814:Larva / Q000254:growth & development; D010504:Periodic Acid / Q000627:therapeutic use; D012698:Serologic Tests / Q000379:methods*; D017171:Strongyloides stercoralis / Q000276:immunology* / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D007074:Immunoglobulin G; D010504:Periodic Acid; D007073:Immunoglobulin E; C009288:metaperiodate