title: High activity catechol 1,2-dioxygenase from Stenotrophomonas maltophilia strain KB2 as a useful tool in cis,cis-muconic acid production.
abstract: This is the first report of a catechol 1,2-dioxygenase from Stenotrophomonas maltophilia strain KB2 with high activity against catechol and its methyl derivatives. This enzyme was maximally active at pH 8.0 and 40 °C and the half-life of the enzyme at this temperature was 3 h. Kinetic studies showed that the value of K m and V max was 12.8 μM and 1,218.8 U/mg of protein, respectively. During our studies on kinetic properties of the catechol 1,2-dioxygenase we observed substrate inhibition at >80 μM. The nucleotide sequence of the gene encoding the S. maltophilia strain KB2 catechol 1,2-dioxygenase has high identity with other catA genes from members of the genus Pseudomonas. The deduced 314-residue sequence of the enzyme corresponds to a protein of molecular mass 34.5 kDa. This enzyme was inhibited by competitive inhibitors (phenol derivatives) only by ca. 30 %. High tolerance against condition changes is desirable in industrial processes. Our data suggest that this enzyme could be of use as a tool in production of cis,cis-muconic acid and its derivatives.
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellonska 28, 40-032, Katowice, Poland. urszula.guzik@us.edu.pl
authors.forename: Urszula
authors.initials: U
authors.lastname: Guzik
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hupert-Kocurek
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sitnik
authors.forename: Danuta
authors.initials: D
authors.lastname: Wojcieszyńska
mesh_terms: D000595:Amino Acid Sequence; D050548:Catechol 1,2-Dioxygenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D020615:Stenotrophomonas maltophilia / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D002396:Catechols; C005939:muconic acid; D050548:Catechol 1,2-Dioxygenase; D013011:Sorbic Acid
references.citation: J Biol Chem. 2004 Jun 25;279(26):27646-55
references.pmid: 15060064
references.citation: Eur J Biochem. 1970 Feb;12(3):427-34
references.pmid: 5462291
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: AMB Express. 2011 May 27;1(1):8
references.pmid: 21906340
references.citation: J Struct Biol. 2010 Jun;170(3):548-64
references.pmid: 20040374
references.citation: Arch Microbiol. 1996 Jul;166(1):42-50
references.pmid: 8661943
references.citation: J Biosci Bioeng. 2004;98(2):71-6
references.pmid: 16233669
references.citation: Microbiol Res. 1998 Aug;153(2):163-71
references.pmid: 9760749
references.citation: J Bacteriol. 1988 Oct;170(10):4874-80
references.pmid: 3170486
references.citation: Biochem Biophys Res Commun. 2005 Dec 9;338(1):198-205
references.pmid: 16171781
references.citation: Crit Rev Biochem Mol Biol. 2006 Jul-Aug;41(4):241-67
references.pmid: 16849108
references.citation: World J Microbiol Biotechnol. 2011 Apr;27(4):805-811
references.pmid: 21475727
references.citation: J Mol Biol. 1994 Dec 16;244(5):586-608
references.pmid: 7990141
references.citation: Int J Biol Macromol. 2010 Aug 1;47(2):233-7
references.pmid: 20452370
references.citation: J Protein Chem. 2000 Nov;19(8):709-16
references.pmid: 11307956
references.citation: J Bacteriol. 1976 Jul;127(1):536-44
references.pmid: 58860
references.citation: Biochemistry (Mosc). 2009 Sep;74(9):994-1001
references.pmid: 19916910
references.citation: Structure. 2000 Apr 15;8(4):429-40
references.pmid: 10801478
references.citation: Biochem J. 1978 Jul 15;174(1):85-94
references.pmid: 697766
references.citation: Biochemistry (Mosc). 2006 Dec;71(12):1316-23
references.pmid: 17223783
references.citation: Chem Soc Rev. 2008 Dec;37(12):2716-44
references.pmid: 19020684
references.citation: Appl Environ Microbiol. 1991 Mar;57(3):812-9
references.pmid: 16348446
references.citation: J Biol Chem. 1998 Dec 25;273(52):34887-95
references.pmid: 9857017