title: [Construction and identification of a recombinant lentiviral expression plasmid pLentiTrident1-hBMP2-Neo-hNGF].
abstract: OBJECTIVE
  To construct and confirm a recombinant lentiviral vector containing human bone morphogenetic protein 2 (hBMP2) and human nerve growth factor (hNGF).
  
  
  METHODS
  The Neomycin gene was digested from pLentiTrident1-EGFP-Neo and then was subcloned into lentiviral vector. The hBMP2 and hNGF genes were amplified by polymerase chain reaction (PCR), and then the PCR product was inserted to proper sites of the vector. Finally, the recombinant vector pLentiTrident1-hBMP2-Neo-hNGF was confirmed by agarose gel electrophoresis and DNA sequence analysis.
  
  
  RESULTS
  The construction of recombinant lentiviral vector pLentiTrident-hBMP2 -Neo-hNGF was confirmed through restriction enzyme maping analysis and DNA sequencing.
  
  
  CONCLUSIONS
  The recombinant lentiviral vector which can coexpress hBMP2 and hNGF is successfully constructed,which lays a solid foundation of studying the effect of neuro factors on bone regeneration.
authors.affiliation: Department of Periodontology, School of Stomatology, Shandong University, Key Lab of Oral Biomedicine of Shandong Province, Jinan 250012, Shandong Province, China. hanqianqian04@163.com
authors.forename: Qian-qian
authors.initials: QQ
authors.lastname: Han
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Ge
authors.forename: Xiang-zhen
authors.initials: XZ
authors.lastname: Yan
authors.forename: Pi-shan
authors.initials: PS
authors.lastname: Yang
mesh_terms: D055396:Bone Morphogenetic Protein 2; D001842:Bone and Bones; D005822:Genetic Vectors; D006801:Humans; D020932:Nerve Growth Factor; D010957:Plasmids; D011994:Recombinant Proteins; D012038:Regeneration; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D020932:Nerve Growth Factor