title: Bacterial cellulose nanofibrillar patch as a wound healing platform of tympanic membrane perforation.
abstract: Bacterial cellulose (BC)-based biomaterials on medical device platforms have gained significant interest for tissue-engineered scaffolds or engraftment materials in regenerative medicine. In particular, BC has an ultrafine and highly pure nanofibril network structure and can be used as an efficient wound-healing platform since cell migration into a wound site is strongly meditated by the structural properties of the extracellular matrix. Here, the fabrication of a nanofibrillar patch by using BC and its application as a new wound-healing platform for traumatic tympanic membrane (TM) perforation is reported. TM perforation is a very common clinical problem worldwide and presents as conductive hearing loss and chronic perforations. The BC nanofibrillar patch can be synthesized from Gluconacetobacter xylinus; it is found that the patch contained a network of nanofibrils and is transparent. The thickness of the BC nanofibrillar patch is found to be approximately 10.33 ± 0.58 μm, and the tensile strength and Young's modulus of the BC nanofibrillar patch are 11.85 ± 2.43 and 11.90 ± 0.48 MPa, respectively, satisfying the requirements of an ideal wound-healing platform for TM regeneration. In vitro studies involving TM cells show that TM cell proliferation and migration are stimulated under the guidance of the BC nanofibrillar patch. In vivo animal studies demonstrate that the BC nanofibrillar patch promotes the rate of TM healing as well as aids in the recovery of TM function. These data demonstrate that the BC nanofibrillar patch is a useful wound-healing platform for TM perforation.
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-742 Republic of Korea.
authors.forename: Jangho
authors.initials: J
authors.lastname: Kim
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Subeom
authors.initials: S
authors.lastname: Park
authors.forename: Ki Taek
authors.initials: KT
authors.lastname: Lim
authors.forename: Hoon
authors.initials: H
authors.lastname: Seonwoo
authors.forename: Yeonju
authors.initials: Y
authors.lastname: Kim
authors.forename: Byung Hee
authors.initials: BH
authors.lastname: Hong
authors.forename: Yun-Hoon
authors.initials: YH
authors.lastname: Choung
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000091:Acetobacter / Q000737:chemistry*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014432:Tympanic Membrane / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D018058:Tympanic Membrane Perforation / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D002482:Cellulose
keywords: bacterial cellulose; nanofibrillar patch; regenerative medicine; tympanic membrane; wound healing