title: Prognostic value of CAPZA1 overexpression in gastric cancer.
abstract: F-actin capping protein α1 subunit (CAPZA1) was previously identified in a proteomic analysis of human gastric cancer clinical specimens and selected for further study. The association between CAPZA1 overexpression, detected by immunohistochemistry, and clinicopathological features including survival were evaluated. In vitro gain-of-function and loss-of-function approaches were utilized to assess the function of CPAZA1 in malignancy. Univariate analysis revealed that poorly differentiated disease, according to the World Health Organization (WHO) classification, advanced T stage, positive lymph nodes, high TNM stage, D2 lymph node dissection, adjuvant chemotherapy and CAPZA1 underexpression were significantly associated with cancer-related death (p<0.05); however, only high TNM stage remained significantly associated by multivariate analysis (p<0.01). CAPZA1 overexpression was associated with well differentiated histology, smaller tumor size, lower T stage, absence of lymph node metastasis, lower TNM stage, lower recurrence rate and longer survival time, compared to CAPZA1 underexpression. In vitro, forced expression of CAPZA1 caused a significant decrease in gastric cancer cell migration and invasion, whereas CAPZA1 depletion had the opposite effect. The present study suggests that CAPZA1 could be a marker of good prognosis in gastric cancer and shows that CAPZA1 is associated with decreased cancer cell migration and invasion.
authors.affiliation: Department of Surgery, Postgraduate School of Medicine, Gyeongnam Regional Cancer Center, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Lee
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Jeong
authors.forename: Soon-Chan
authors.initials: SC
authors.lastname: Hong
authors.forename: Bok-Im
authors.initials: BI
authors.lastname: Cho
authors.forename: Woo-Song
authors.initials: WS
authors.lastname: Ha
authors.forename: Soon-Tae
authors.initials: ST
authors.lastname: Park
authors.forename: Sang-Kyung
authors.initials: SK
authors.lastname: Choi
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Jung
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ju
authors.forename: Chi-Young
authors.initials: CY
authors.lastname: Jeong
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Lee
authors.forename: Jiyun
authors.initials: J
authors.lastname: Yoo
authors.forename: Gyung Hyuck
authors.initials: GH
authors.lastname: Ko
mesh_terms: D000368:Aged; D051347:CapZ Actin Capping Protein / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008197:Lymph Node Excision; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D013274:Stomach Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology
chemical_list: C495842:CAPZA1 protein, human; D051347:CapZ Actin Capping Protein
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