title: Influence of bacteriophage preparations on migration of HL-60 leukemia cells in vitro.
abstract: BACKGROUND
  Bacteriophage therapy is considered one of the most attractive alternatives to antibiotic treatment, which may be significant due to the rising number of antibiotic-resistant bacterial strains. Patients with cancer frequently suffer bacterial infections resulting from immunosuppression caused by anticancer treatment; thus they constitute a considerable group of patients subjected to phage therapy. In this study, we investigated the influence of bacteriophages on the migration of human leukemia (HL-60) cells. Results of these studies provide data regarding phage treatment of patients with cancer, especially with this type of leukemia.
  
  
  METHODS
  The influence of phage preparation on migration of HL-60 leukemia cells was evaluated with BD Bioscience Migration Chambers.
  
  
  RESULTS
  Bacteriophages have no influence on migration of HL-60 cells. The only phage preparation which stimulated migration of HL-60 cells was Staph.liz, specific to S. aureus, however, the molecular basis of these interactions cannot be currently explained.
  
  
  CONCLUSIONS
  Results of our studies may be in line with previous data indicating that phage therapy is safe for patients with cancer.
authors.affiliation: Laboratory of Bacteriophages, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. aneta.kurzepa@up.wroc.pl
authors.forename: Aneta
authors.initials: A
authors.lastname: Kurzepa-Skaradzinska
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Skaradzinski
authors.forename: Beata
authors.initials: B
authors.lastname: Weber-Dabrowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Zaczek
authors.forename: Tomasz
authors.initials: T
authors.lastname: Maj
authors.forename: Anna
authors.initials: A
authors.lastname: Slawek
authors.forename: Marta
authors.initials: M
authors.lastname: Switalska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Maciejewska
authors.forename: Joanna
authors.initials: J
authors.lastname: Wietrzyk
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rymowicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gorski
mesh_terms: D001435:Bacteriophages / Q000502:physiology*; D002465:Cell Movement; D004926:Escherichia coli / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology / Q000628:therapy*; D018922:HL-60 Cells; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000378:metabolism; D011552:Pseudomonas Infections / Q000382:microbiology / Q000628:therapy*; D011550:Pseudomonas aeruginosa / Q000502:physiology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000628:therapy*; D013211:Staphylococcus aureus / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides