title: Alteration of intracellular calcium and its modulator SLC24A6 after experimental intracerebral hemorrhage.
abstract: Intracerebral hemorrhage (ICH) can lead to tragic disability and mortality. Accumulating evidence has shown that sodium calcium exchanger (NCX) may contribute to the secondary injury of a stroke. Recently, a novel member of NCX, SLC24A6, was discovered with knowledge of its abundant distribution in brain. In the present study, we examined the time course of expression of SLC24A6 and its mediated intracellular calcium concentration ([Ca(2+)]i) to investigate its potential roles in brain damage after ICH. An ICH model was established as previously reported. Real-time PCR and Western blotting were used to test the mRNA and protein levels of SLC24A6 on the hemorrhagic side and on the contralateral side caudate nucleus tissues at 6 h, and on days 1, 3, 5, and 7 after ICH. Immunohistochemistry was used to analyze the morphological changes. Fura-2/AM loaded, dual wavelength spectrophotofluorometry was used to test [Ca(2+)]i. The data presented a remarkable decrease in SLC24A6 early after ICH, along with a comparable increase in [Ca(2+)]i. Our results indicated that SLC24A6 presents specific and remarkable alterations in both mRNA and protein levels after ICH. Decreases in SLC24A6 level were correlated with [Ca(2+)]i elevation. These data suggest that SLC24A6-mediated calcium overload plays an important role in brain damage after ICH.
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Zheng
authors.forename: Ye
authors.initials: Y
authors.lastname: Gong
authors.forename: Xuanchun
authors.initials: X
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.forename: Hailiang
authors.initials: H
authors.lastname: Tang
authors.forename: Daijun
authors.initials: D
authors.lastname: Wang
authors.forename: Xiancheng
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002118:Calcium / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000150:complications* / Q000473:pathology*; D004195:Disease Models, Animal; D016257:Fura-2 / Q000031:analogs & derivatives; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000378:metabolism*; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D019831:Sodium-Calcium Exchanger; C049925:fura-2-am; D002118:Calcium; D016257:Fura-2