title: Crude glycerol-based production of amino acids and putrescine by Corynebacterium glutamicum.
abstract: Corynebacterium glutamicum possesses genes for glycerol kinase and glycerol-3-phosphate dehydrogenase that were shown to support slow growth with glycerol only when overexpressed from a plasmid. Pure glycerol and crude glycerol from biodiesel factories were tested for growth of recombinant strains expressing glpF, glpK and glpD from Escherichia coli. Some, but not all crude glycerol lots served as good carbon sources. Although the inhibitory compound(s) present in these crude glycerol lots remained unknown, the addition of substoichiometric glucose concentrations (below 10% by weight) enabled the utilization of some of the inhibitory crude glycerol lots. Besides growth, production of the amino acids L-glutamate, L-lysine, L-ornithine and L-arginine as well as of the diamine putrescine based on crude glycerol qualities from biodiesel factories was demonstrated.
authors.affiliation: Chair of Genetics of Prokaryotes, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Meiswinkel
authors.forename: Doris
authors.initials: D
authors.lastname: Rittmann
authors.forename: Steffen N
authors.initials: SN
authors.lastname: Lindner
authors.forename: Volker F
authors.initials: VF
authors.lastname: Wendisch
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis*; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism; D056804:Biofuels / Q000032:analysis*; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011700:Putrescine / Q000096:biosynthesis*
chemical_list: D000596:Amino Acids; D020346:Aquaporins; D056804:Biofuels; D017931:DNA Primers; D029968:Escherichia coli Proteins; C078043:GlpF protein, E coli; D005993:Glycerolphosphate Dehydrogenase; C098831:glycerol-3-phosphate oxidase; D005990:Glycerol; D011700:Putrescine
keywords: Biodiesel; Corynebacterium glutamicum; Putrescine; Raw glycerol; l-amino acids