title: Enzymatic preparation and structural determination of oligosaccharides derived from sea cucumber (Acaudina molpadioides) fucoidan.
abstract: Sea cucumber fucoidan is a major bioactive component of sea cucumber. Sea cucumber is widely consumed in East Asian countries as healthy food. Employing the degrading enzyme from the marine bacterium strain Flavobacteriaceae CZ1127, sea cucumber (Acaudina molpadioides) fucoidan oligosaccharides were prepared by enzymatic hydrolysis. The oligosaccharide profile of the hydrolysate was determined by liquid chromatography coupled with mass spectrometry (LC-MS). With the assistance of LC-MS, four major oligosaccharides in the hydrolysate were purified. By using tandem mass spectrometry and nuclear magnetic resonance, delicate structures of the oligosaccharides were verified as α-l-Fucp-1→3-α-l-Fucp(2,4OSO3(2-))-1→3-α-l-Fucp, α-l-Fucp-1→3-α-l-Fucp(2,4OSO3(2-))-1→3-α-l-Fucp-1→3-α-l-Fucp, α-l-Fucp-1→3-α-l-Fucp(2,4OSO3(2-))-1→3-α-l-Fucp-1→3-α-l-Fucp-1→3-α-l-Fucp-1→3-α-l-Fucp(2,4OSO3(2-))-1→3-α-l-Fucp and α-l-Fucp-1→3-α-l-Fucp(2,4OSO3(2-))-1→3-α-l-Fucp-1→3-α-l-Fucp-1→3-α-l-Fucp-1→3-α-l-Fucp(2,4OSO3(2-))-1→3-α-l-Fucp-1→3-α-l-Fucp.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Xu
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: Chang
authors.forename: Lei
authors.initials: L
authors.lastname: Ge
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Wang
authors.forename: Cuiyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Guanchen
authors.initials: G
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: He
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D002240:Carbohydrate Sequence; D041965:Flavobacteriaceae / Q000201:enzymology*; D058870:Food, Organic / Q000032:analysis*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012616:Sea Cucumbers / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D011134:Polysaccharides; C007789:fucoidan