title: Transcriptomic and cellular response to bacterial challenge (pathogenic Vibrio parahaemolyticus) in farmed juvenile Haliotis rufescens fed with or without probiotic diet.
abstract: The abalone production in Chile has increased considerably in recent years with no sign of tapering off. Open and semi-closed circuits in the marine water zones in the north and south of Chile are the preferred areas of culture. Coastal ecosystems are subjected to a wide variety of contaminants that generate stress that affects populations via their impacts to individuals at both physiological and genetic levels. This work investigated the genomic and cellular response of post-weaning juvenile Haliotis rufescens abalone under hatchery conditions, fed with probiotic diets, and subsequently challenged with Vibrio parahaemolyticus. The expression patterns of 16 selected genes associated with different metabolic pathways were analyzed using Real-Time PCR. Gene expression was then compared to immunological response parameters in the abalone and quantification of V. parahaemolyticus during the experimental period. Both transcriptomic and immunological analyses indicated significant alteration of physiological processes in H. rufescens correlated to exposure to the pathogenic bacteria, as well as to probiotic nutrition.
authors.affiliation: Laboratorio de Ecología Microbiana, Universidad de Antofagasta, 170 Antofagasta, Chile. fsaciares@uantof.cl
authors.forename: Fernando
authors.initials: F
authors.lastname: Silva-Aciares
authors.forename: Dario
authors.initials: D
authors.lastname: Moraga
authors.forename: Michel
authors.initials: M
authors.lastname: Auffret
authors.forename: Arnaud
authors.initials: A
authors.lastname: Tanguy
authors.forename: Carlos
authors.initials: C
authors.lastname: Riquelme
mesh_terms: D000818:Animals; D004032:Diet; D049851:Gastropoda / Q000187:drug effects / Q000382:microbiology* / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D054884:Host-Pathogen Interactions / Q000235:genetics*; D010587:Phagocytosis / Q000187:drug effects*; D019936:Probiotics / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects; D014736:Vibrio parahaemolyticus / Q000502:physiology*