title: Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes.
abstract: In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL(-1) PC2 and 100 µg·mL(-1) of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. miguelangel.merlos@eez.csic.es
authors.forename: Miguel Angel Merlos
authors.initials: MA
authors.lastname: Rodrigo
authors.forename: Natalia
authors.initials: N
authors.lastname: Cernei
authors.forename: Marketa
authors.initials: M
authors.lastname: Kominkova
authors.forename: Ondrej
authors.initials: O
authors.lastname: Zitka
authors.forename: Miroslava
authors.initials: M
authors.lastname: Beklova
authors.forename: Josef
authors.initials: J
authors.lastname: Zehnalek
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
mesh_terms: D002104:Cadmium / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000737:chemistry*; D054811:Phytochelatins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004785:Environmental Pollutants; D002104:Cadmium; D054811:Phytochelatins
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