title: Protective role of the endoplasmic reticulum protein mitsugumin23 against ultraviolet C-induced cell death.
abstract: The endoplasmic reticulum (ER) operates in adaptive responses to various stresses, dictating cell fate. Here we show that knockdown of the ER protein mitsugumin23 (MG23) enhances cell death induced by ultraviolet C (UVC), which causes DNA damage. The small heat shock protein αB-crystallin (αBC) is identified as a MG23 binding molecule and its knockdown facilitates death of UVC-exposed cells. Conversely, αBC lowered UVC sensitivity when expressed as an ER-anchored form. Taken together, the results suggest that MG23 plays a protective role against UVC by accumulating αBC in the close vicinity of the ER.
authors.affiliation: Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8505, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Yamashita
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Taniwaki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kaikoi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yamazaki
mesh_terms: D000818:Animals; D016923:Cell Death / Q000528:radiation effects; D004249:DNA Damage; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000528:radiation effects; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D038203:alpha-Crystallin B Chain / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D038203:alpha-Crystallin B Chain; C547817:mitsugumin23 protein, human; C547818:mitsugumin23 protein, mouse