title: Prediction of Brugia malayi antigenic peptides: candidates for synthetic vaccine design against lymphatic filariasis.
abstract: Brugia malayi is a threadlike nematode cause's swelling of lymphatic organs, condition well known as lymphatic filariasis; till date no invention made to effectively address lymphatic filariasis. In this analysis we a have predicted suitable antigenic peptides from Brugia malayi antigen protein for peptide vaccine design against lymphatic filariasis based on cross protection phenomenon as, an ample immune response can be generated with a single protein subunit. We found MHC class II binding peptides of Brugia malayi antigen protein are important determinant against the diseased condition. The analysis shows Brugia malayi antigen protein having 505 amino acids, which shows 497 nonamers. In this assay, we have predicted MHC-I binding peptides for 8mer_H2_Db (optimal score- 15.966), 9mer_H2_Db (optimal score- 15.595), 10mer_H2_Db (optimal score- 19.405), 11mer_H2_Dballeles (optimal score- 23.801). We also predicted the SVM based MHCII-IAb nonamers, 51-FQQIDPLDA, 442-FAAIACLVH, 206-YLNPFGHQF, 167-WYVIMAACY, 367-YAMIVIRLL, 434- LVITTAANF, 176-LDSYCLWKP, 435-VITTAANFA, 364-WPGYAMIVI (optimal score- 13.963); MHCII-IAd nonamers, 52-QQIDPLDAE, 171-MAACYLDSY, 239-QWRSVILCN, 168-YVIMAACYL, 3-QYLSVHSLS, 322-EILLHAKVV, 417- LGIIASFVS, 396-KAIFLAHFG, 167-WYVIMAACY, 269-LALHCINVI, 93-FINKAAPKQ, 259-NCIIVLKAF, 79- QGVLLIIPR, 22-TILQRSQAI, 63-RGFVYGNVS, 109-NISSLAFET,(optimal score- 16.748); and MHCII-IAg7 nonamers 171-MAACYLDSY, 73-KIVNGAQGV, 259-NCIIVLKAF, 209-PFGHQFSFE, 102-SCDTLLKNI, 25-QRSQAIRIV, 444- AIACLVHLF, 88-SLVNGFINK, 252-FPRHQLLNC, 471-RFVLANDNE, 52-QQIDPLDAE, 469-HRRFVLAND, 457- SNRHYFLAD, 362-KSWPGYAMI, 476-NDNEGEDFE, 370-IVIRLLQAL (optimal score- 19.847) which represents potential binders from Brugia malayi antigen protein. The method integrates prediction of MHC class I binding proteasomal C-terminal cleavage peptides and Eighteen potential antigenic peptides at average propensity 1.063 having highest local hydrophilicity. Thus a small antigen fragment can induce immune response against whole antigen. This approach can be applied for designing subunit and synthetic peptide vaccines.
authors.affiliation: Department of Bioinformatics, Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu-333001 Rajasthan, India. gomase.viren@gmail.com
authors.forename: Virendra S
authors.initials: VS
authors.lastname: Gomase
authors.forename: Nikhilkumar R
authors.initials: NR
authors.lastname: Chitlange
authors.forename: Smruti S
authors.initials: SS
authors.lastname: Changbhale
authors.forename: Karbhari V
authors.initials: KV
authors.lastname: Kale
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000627:therapeutic use; D001665:Binding Sites / Q000235:genetics; D017178:Brugia malayi / Q000235:genetics / Q000276:immunology*; D015195:Drug Design; D004605:Elephantiasis, Filarial / Q000276:immunology / Q000517:prevention & control*; D000939:Epitopes / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D017433:Protein Structure, Secondary; D020539:Sequence Analysis, Protein; D014614:Vaccines, Synthetic / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D000947:Antigens, Helminth; D000939:Epitopes; C408902:MHC binding peptide; D009842:Oligopeptides; D014614:Vaccines, Synthetic