title: Dynamic interplay of ParA with the polarity protein, Scy, coordinates the growth with chromosome segregation in Streptomyces coelicolor.
abstract: Prior to bacterial cell division, the ATP-dependent polymerization of the cytoskeletal protein, ParA, positions the newly replicated origin-proximal region of the chromosome by interacting with ParB complexes assembled on parS sites located close to the origin. During the formation of unigenomic spores from multi-genomic aerial hyphae compartments of Streptomyces coelicolor, ParA is developmentally triggered to form filaments along the hyphae; this promotes the accurate and synchronized segregation of tens of chromosomes into prespore compartments. Here, we show that in addition to being a segregation protein, ParA also interacts with the polarity protein, Scy, which is a component of the tip-organizing centre that controls tip growth. Scy recruits ParA to the hyphal tips and regulates ParA polymerization. These results are supported by the phenotype of a strain with a mutant form of ParA that uncouples ParA polymerization from Scy. We suggest that the ParA-Scy interaction coordinates the transition from hyphal elongation to sporulation.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Ditkowski
authors.forename: Neil
authors.initials: N
authors.lastname: Holmes
authors.forename: Joanna
authors.initials: J
authors.lastname: Rydzak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Donczew
authors.forename: Martyna
authors.initials: M
authors.lastname: Bezulska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Ginda
authors.forename: Pawel
authors.initials: P
authors.lastname: Kedzierski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zakrzewska-Czerwińska
authors.forename: Gabriella H
authors.initials: GH
authors.lastname: Kelemen
authors.forename: Dagmara
authors.initials: D
authors.lastname: Jakimowicz
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D048372:Streptomyces coelicolor / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; C052883:chromosome partition proteins, bacterial
references.citation: Curr Opin Struct Biol. 2007 Feb;17(1):103-9
references.pmid: 17161598
references.citation: Mol Microbiol. 2011 Feb;79(4):1089-100
references.pmid: 21235642
references.citation: Nat Rev Microbiol. 2009 Jan;7(1):36-49
references.pmid: 19079351
references.citation: J Bacteriol. 2008 Nov;190(22):7579-83
references.pmid: 18805980
references.citation: J Bacteriol. 2006 Mar;188(5):1710-20
references.pmid: 16484182
references.citation: Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14194-8
references.pmid: 20660743
references.citation: J Bacteriol. 2005 May;187(10):3572-80
references.pmid: 15866947
references.citation: Mol Microbiol. 2007 Aug;65(3):625-41
references.pmid: 17635186
references.citation: EMBO J. 2005 Jan 26;24(2):270-82
references.pmid: 15635448
references.citation: Mol Cell. 2002 Aug;10(2):427-34
references.pmid: 12191487
references.citation: Mol Microbiol. 2008 Nov;70(4):1037-50
references.pmid: 18976278
references.citation: Nat Cell Biol. 2010 Aug;12(8):791-8
references.pmid: 20657594
references.citation: Mol Microbiol. 2007 Jan;63(2):468-81
references.pmid: 17166176
references.citation: Mol Microbiol. 2012 Aug;85(3):393-404
references.pmid: 22646484
references.citation: Mol Microbiol. 1997 Sep;25(5):847-58
references.pmid: 9364911
references.citation: Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):E397-406
references.pmid: 23297235
references.citation: Nat Rev Microbiol. 2006 Feb;4(2):133-43
references.pmid: 16415929
references.citation: Mol Microbiol. 2004 Apr;52(2):385-98
references.pmid: 15066028
references.citation: Mol Microbiol. 2002 Sep;45(5):1365-77
references.pmid: 12207703
references.citation: J Bacteriol. 2011 Mar;193(5):1273-5
references.pmid: 21193604
references.citation: Proc Natl Acad Sci U S A. 1998 May 12;95(10):5752-6
references.pmid: 9576956
references.citation: Curr Opin Microbiol. 2010 Dec;13(6):758-65
references.pmid: 21036658
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1541-6
references.pmid: 12563033
references.citation: EMBO J. 2005 Apr 6;24(7):1453-64
references.pmid: 15775965
references.citation: EMBO J. 2010 Sep 15;29(18):3068-81
references.pmid: 20802464
references.citation: Microbiology (Reading). 1999 Sep;145 ( Pt 9):2229-2243
references.pmid: 10517576
references.citation: Mol Microbiol. 2010 Dec;78(6):1403-15
references.pmid: 21143314
references.citation: Sci Rep. 2011;1:156
references.pmid: 22355671
references.citation: Mol Microbiol. 2012 Jun;84(6):1033-49
references.pmid: 22582857
references.citation: Philos Trans R Soc Lond B Biol Sci. 2005 Mar 29;360(1455):523-35
references.pmid: 15897178
references.citation: Genes Dev. 2006 Dec 1;20(23):3269-82
references.pmid: 17158745
references.citation: Curr Opin Microbiol. 2012 Dec;15(6):737-43
references.pmid: 23153774
references.citation: J Struct Biol. 2010 May;170(2):202-15
references.pmid: 20178847
references.citation: Cell. 2010 Jun 11;141(6):927-42
references.pmid: 20550930
references.citation: Mol Microbiol. 2012 Apr;84(1):105-16
references.pmid: 22340668