title: TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats.
abstract: The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-1N) resembling human mesangioproliferative glomerulonephritis have been explored for many years; however, the molecular mechanisms of GMC proliferation and ECM production remain unclear. Our previous studies have demonstrated that the thrombospondin-1 (TSP-1) gene was involved in mediating rat GMC proliferation and ECM synthesis induced by sublytic C5b-9 in vitro. In the present study, the roles of the TSP-1 gene in GMC proliferation, ECM production, and urinary protein secretion in Thy-1N rats were determined by using TSP-1 small hairpin RNA, and the results revealed that silencing of the TSP-1 gene in rat renal tissues could diminish GMC proliferation (P < 0.01) and ECM secretion (P < 0.01) as well as urinary protein secretion (P < 0.05) in Thy-1N rats. Together, the current findings suggested that TSP-1 gene expression was required for GMC proliferation and ECM production in Thy-1N rats.
authors.affiliation: Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors.forename: Wen
authors.initials: W
authors.lastname: Qiu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhou
authors.forename: Chenhui
authors.initials: C
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Shan
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Wang
keywords: Thy-1 nephritis; extracellular matrix; glomerular mesangial cells (GMCs); proliferation; thrombospondin-1 (TSP-1)
references.citation: Clin J Am Soc Nephrol. 2007 Sep;2(5):1054-61
references.pmid: 17702711
references.citation: Int J Biochem Cell Biol. 2004 Jun;36(6):1102-14
references.pmid: 15094125
references.citation: Contrib Nephrol. 2007;157:56-63
references.pmid: 17495438
references.citation: Kidney Int. 1996 Feb;49(2):335-43
references.pmid: 8821815
references.citation: Immunol Res. 2005;31(1):37-46
references.pmid: 15591621
references.citation: Nephron Exp Nephrol. 2009;111(3):e61-6
references.pmid: 19182492
references.citation: Clin Sci (Lond). 2003 May;104(5):455-66
references.pmid: 12580763
references.citation: Curr Opin Nephrol Hypertens. 2005 May;14(3):223-8
references.pmid: 15821414
references.citation: Histol Histopathol. 2009 Jul;24(7):901-8
references.pmid: 19475536
references.citation: Hepatobiliary Pancreat Dis Int. 2007 Aug;6(4):424-9
references.pmid: 17690043
references.citation: Eur J Immunol. 2009 Nov;39(11):3251-66
references.pmid: 19882653
references.citation: Kidney Int. 2005 Sep;68(3):1086-95
references.pmid: 16105039
references.citation: J Histochem Cytochem. 2008 Jan;56(1):15-24
references.pmid: 17875657
references.citation: J Lab Clin Med. 2006 Jun;147(6):301-9
references.pmid: 16750668
references.citation: Am J Physiol Endocrinol Metab. 2007 Nov;293(5):E1459-64
references.pmid: 17726142
references.citation: Kidney Int. 1987 Oct;32(4):514-25
references.pmid: 2892961
references.citation: J Immunol. 2006 Mar 1;176(5):3173-80
references.pmid: 16493077
references.citation: J Immunol. 1987 Jun 1;138(11):3758-65
references.pmid: 3495569
references.citation: Am J Physiol Renal Physiol. 2010 Mar;298(3):F579-88
references.pmid: 20032116
references.citation: FASEB J. 2010 Jun;24(6):1925-34
references.pmid: 20124433
references.citation: Int J Biochem Cell Biol. 2004 Jun;36(6):1115-25
references.pmid: 15094126
references.citation: J Biol Chem. 2006 Jul 14;281(28):19009-18
references.pmid: 16670089
references.citation: Cell Mol Life Sci. 2008 Mar;65(5):672-86
references.pmid: 18193164
references.citation: Microvasc Res. 2007 Sep-Nov;74(2-3):90-9
references.pmid: 17559888
references.citation: J Am Soc Nephrol. 1999 Jun;10(6):1242-52
references.pmid: 10361862
references.citation: Clin Exp Immunol. 2006 May;144(2):326-34
references.pmid: 16634807
references.citation: Am J Pathol. 2003 Sep;163(3):1185-92
references.pmid: 12937160
references.citation: Am J Physiol Renal Physiol. 2009 Jan;296(1):F186-93
references.pmid: 18987115
references.citation: Scand J Urol Nephrol. 2008;42(4):373-80
references.pmid: 19230171
references.citation: Kidney Int. 2006 Oct;70(7):1251-60
references.pmid: 16900094
references.citation: Cell Tissue Res. 2006 Oct;326(1):159-68
references.pmid: 16736195
references.citation: Cell Prolif. 2009 Feb;42(1):83-93
references.pmid: 19143766
references.citation: Nephron. 2000 Oct;86(2):152-60
references.pmid: 11014985
references.citation: J Surg Res. 2004 Nov;122(1):135-42
references.pmid: 15522326
references.citation: Life Sci. 2006 Jun 6;79(2):182-92
references.pmid: 16458938