title: Preparative separation of triterpene alcohol ferulates from rice bran oil using a high performance counter-current chromatography.
abstract: A novel method for the separation of two major triterpene alcohol ferulates from rice bran oil (RBO) was developed using a high performance counter-current chromatography (HPCCC). A two-phase solvent system of n-hexane-acetonitrile (1:1, v/v) was applied to purify cycloartenyl ferulate (CAF) and 24-methylene cycloartanyl ferulate (24-mCAF) from RBO. The yields were 20.50±2.60 mg CAF and 12.62±1.15 mg 24-mCAF from 390 mg RBO through a two-step separation procedure. The purities of the two compounds were 97.97±0.90% and 95.50±0.75%, respectively, as determined by high performance liquid chromatography (HPLC). Their chemical structures were confirmed by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and (1)H, (13)C and 2D nuclear magnetic resonance (NMR). This represents the first report on direct separation of CAF and 24-mCAF from RBO by HPCCC.
authors.affiliation: Institute of Food and Nutraceutical Science, Key Lab of Urban Agriculture (South), School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Man
authors.initials: M
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Haiming
authors.initials: H
authors.lastname: Shi
authors.forename: Casimir C
authors.initials: CC
authors.lastname: Akoh
authors.forename: Liangli Lucy
authors.initials: LL
authors.lastname: Yu
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D000073879:Rice Bran Oil; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D010938:Plant Oils; D014315:Triterpenes; D000073879:Rice Bran Oil