title: Radioiodide induces apoptosis in human thyroid tissue in culture.
abstract: Radioiodide ((131)I) is routinely used for the treatment of toxic adenoma, Graves' disease, and for ablation of thyroid remnant after thyroidectomy in patients with thyroid cancer. The toxic effects of ionizing radiations on living cells can be mediated by a necrotic and/or apoptotic process. The involvement of apoptosis in radiation-induced cell death in the thyrocytes has been questioned. The knowledge of the mechanisms that underlie the thyrocyte death in response to radiations can help to achieve a successful treatment with the lowest (131)I dose. We developed a method to study the effects of (131)I in human thyroid tissue in culture, by which we demonstrated that (131)I induces thyroid cell apoptosis. Human thyroid tissues of about 1 mm(3) were cultured in vitro and cell viability was determined up to 3 weeks by the MTT assay. Radioiodide added to the culture medium was actively taken up by the tissues. The occurrence of apoptosis in the thyrocytes was assessed by measuring the production of a caspase-cleavage fragment of cytokeratin 18 (M30) by an enzyme-linked immunoassay. Neither variation of cell number nor spontaneous apoptosis was revealed after 1 week of culture. (131)I added to the culture medium induced a dose-dependent and a time-dependent generation of M30 fragment. The apoptotic process was confirmed by the generation of caspase-3 and PARP cleavage products. These results demonstrate that (131)I induces apoptosis in human thyrocytes. Human thyroid tissue cultures may be useful to investigate the cell death pathways induced by (131)I.
authors.affiliation: Department of Cellular and Molecular Biology and Pathology, University "Federico II", 80131, Naples, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Russo
authors.forename: Anna
authors.initials: A
authors.lastname: Guerra
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Marotta
authors.forename: Antongiulio
authors.initials: A
authors.lastname: Faggiano
authors.forename: Annamaria
authors.initials: A
authors.lastname: Colao
authors.forename: Silvana
authors.initials: S
authors.lastname: Del Vecchio
authors.forename: Massimo
authors.initials: M
authors.lastname: Tonacchera
authors.forename: Mario
authors.initials: M
authors.lastname: Vitale
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007457:Iodine Radioisotopes / Q000494:pharmacology*; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007457:Iodine Radioisotopes; D053148:Caspase 3
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