title: Persistence of Eimeria bovis in soil.
abstract: Eimeriosis is a disease that occurs globally and often affects cattle grazing on pastures contaminated with oocysts of the pathogenic species Eimeria bovis, Eimeria zuernii or Eimeria alabamensis, respectively. Nonetheless, little is understood regarding oocyst persistence on the pasture. The study was performed in the temperate climate zone. Soil samples were spiked with 100,000 E. bovis oocysts in July 2010 or with 50,000 oocysts in October 2010, respectively, both either with our without addition of cattle faeces. The soil samples were exposed to natural environmental conditions until April 2011. A subset of the samples was analysed immediately after spiking as positive control. The oocysts were recovered by a flotation method and counted in a reading chamber. On average, 23 % of the oocysts could be recovered from the positive control. The recovery of oocysts dropped to 0.30 % of the original level in the samples prepared in July independent of the addition of faeces, whereas the oocyst count was higher in the samples prepared in October, both without (2.05 %) and with (2.64 %) faecal material. No differences were observed between presence of oocysts or oocyst counts recovered in the presence or absence of faeces. Presence of faeces had a positive influence on oocyst integrity. During the winter season, the number of oocysts in the soil was lowered under the detection limit in most samples. On the other hand, the comparatively short 3-month summer period had a significant influence on the number of persisting oocysts too.
authors.affiliation: Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51014 Tartu, Estonia. brian.lassen@gmail.com
authors.forename: Brian
authors.initials: B
authors.lastname: Lassen
authors.forename: Triin
authors.initials: T
authors.lastname: Lepik
authors.forename: Berit
authors.initials: B
authors.lastname: Bangoura
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival; D004539:Eimeria / Q000302:isolation & purification* / Q000502:physiology*; D005243:Feces / Q000469:parasitology; D059208:Parasite Load; D012621:Seasons; D012987:Soil / Q000469:parasitology*
chemical_list: D012987:Soil
references.citation: Vet Parasitol. 2006 Dec 20;142(3-4):197-206
references.pmid: 16971047
references.citation: Appl Environ Microbiol. 2002 Nov;68(11):5387-93
references.pmid: 12406729
references.citation: J Parasitol. 2006 Oct;92(5):1115-7
references.pmid: 17152965
references.citation: J Am Vet Med Assoc. 1962 Sep 15;141:706-9
references.pmid: 13881890
references.citation: Vet Parasitol. 2011 Jan 10;175(1-2):135-40
references.pmid: 20943321
references.citation: J Vet Med B Infect Dis Vet Public Health. 2005 Dec;52(10):417-27
references.pmid: 16364016
references.citation: Appl Environ Microbiol. 2001 Dec;67(12):5526-9
references.pmid: 11722902
references.citation: Vet Parasitol. 1984 Sep;15(3-4):213-21
references.pmid: 6541836
references.citation: Vet Parasitol. 2008 May 31;153(3-4):197-202
references.pmid: 18372114
references.citation: Am J Vet Res. 1967 May;28(124):659-65
references.pmid: 6036366
references.citation: Parasitol Res. 2011 May;108(5):1171-7
references.pmid: 21110042
references.citation: Vet Parasitol. 1995 Jan;56(1-3):7-16
references.pmid: 7732653
references.citation: Parasitol Res. 2009 Oct;105(5):1211-22
references.pmid: 19557434
references.citation: Vet Parasitol. 2011 Dec 29;183(1-2):59-67
references.pmid: 21764217
references.citation: Vet Parasitol. 1994 May;53(1-2):23-32
references.pmid: 8091615
references.citation: J Parasitol. 1998 Aug;84(4):862-5
references.pmid: 9714227
references.citation: Microb Ecol. 2004 May;47(4):359-65
references.pmid: 14749905
references.citation: Acta Vet Scand. 1995;36(1):9-20
references.pmid: 7572462
references.citation: Appl Environ Microbiol. 1996 Apr;62(4):1431-3
references.pmid: 8919806
references.citation: Appl Parasitol. 1996 Sep;37(3):209-16
references.pmid: 8856947
references.citation: Vet Parasitol. 2006 Mar 31;136(3-4):215-21
references.pmid: 16387444
references.citation: Vet Parasitol. 1997 May;69(3-4):211-8
references.pmid: 9195731
references.citation: Vet Parasitol. 1994 May;53(1-2):33-43
references.pmid: 8091616
references.citation: Vet Parasitol. 2009 May 12;161(3-4):302-6
references.pmid: 19251369
references.citation: Vet Parasitol. 2008 Feb 14;151(2-4):133-8
references.pmid: 18096320