title: Sugar promotes vegetative phase change in Arabidopsis thaliana by repressing the expression of MIR156A and MIR156C.
abstract: Nutrients shape the growth, maturation, and aging of plants and animals. In plants, the juvenile to adult transition (vegetative phase change) is initiated by a decrease in miR156. In Arabidopsis, we found that exogenous sugar decreased the abundance of miR156, whereas reduced photosynthesis increased the level of this miRNA. This effect was correlated with a change in the timing of vegetative phase change, and was primarily attributable to a change in the expression of two genes, MIR156A and MIR156C, which were found to play dominant roles in this transition. The glucose-induced repression of miR156 was dependent on the signaling activity of HEXOKINASE1. We also show that the defoliation-induced increase in miR156 levels can be suppressed by exogenous glucose. These results provide a molecular link between nutrient availability and developmental timing in plants, and suggest that sugar is a component of the leaf signal that mediates vegetative phase change. DOI:http://dx.doi.org/10.7554/eLife.00260.001.
authors.affiliation: Department of Biology , University of Pennsylvania , Philadelphia , United States.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Mingli
authors.initials: M
authors.lastname: Xu
authors.forename: Yeonjong
authors.initials: Y
authors.lastname: Koo
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.forename: R Scott
authors.initials: RS
authors.lastname: Poethig
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D016922:Cellular Senescence; D018506:Gene Expression Regulation, Plant; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D035683:MicroRNAs; C559759:Mirn156 microRNA, Arabidopsis; D012333:RNA, Messenger; D018749:RNA, Plant; D006593:Hexokinase; C473630:ATHXK1 protein, Arabidopsis; D005947:Glucose
keywords: Arabidopsis; Nicotiana benthamiana; heteroblasty; heterochrony; miRNAs; nutrition; phase change
references.citation: Development. 2011 Jan;138(2):245-9
references.pmid: 21148189
references.citation: Science. 1990 Nov 16;250(4983):923-30
references.pmid: 17746915
references.citation: Development. 1997 Feb;124(3):645-54
references.pmid: 9043079
references.citation: Plant Cell Physiol. 2002 Jun;43(6):684-8
references.pmid: 12091723
references.citation: Curr Opin Plant Biol. 2010 Jun;13(3):274-9
references.pmid: 20056477
references.citation: Development. 2006 Sep;133(18):3539-47
references.pmid: 16914499
references.citation: Plant Cell Environ. 2010 Dec;33(12):2084-100
references.pmid: 20636491
references.citation: Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10507-11
references.pmid: 10468639
references.citation: EMBO Rep. 2006 Oct;7(10):1052-8
references.pmid: 16977334
references.citation: Science. 2003 Apr 11;300(5617):332-6
references.pmid: 12690200
references.citation: Plant Physiol. 2010 Sep;154(1):357-72
references.pmid: 20631317
references.citation: Cell. 2009 Aug 21;138(4):750-9
references.pmid: 19703400
references.citation: Development. 2011 Feb;138(4):677-85
references.pmid: 21228003
references.citation: Cell. 2006 Nov 3;127(3):579-89
references.pmid: 17081979
references.citation: Plant J. 2006 Sep;47(6):841-50
references.pmid: 16889646
references.citation: Adv Morphog. 1967;6:127-71
references.pmid: 4894125
references.citation: New Phytol. 1997 Jan;135(1):59-66
references.pmid: 33863157
references.citation: Development. 2002 Jan;129(1):265-73
references.pmid: 11782419
references.citation: Tree Physiol. 2000 Jun;20(12):815-823
references.pmid: 12651502
references.citation: Trends Plant Sci. 2008 Jul;13(7):343-9
references.pmid: 18502167
references.citation: Plant Physiol. 2005 Aug;138(4):2145-54
references.pmid: 16040653
references.citation: Plant Physiol. 1988 Apr;86(4):1095-8
references.pmid: 16666037
references.citation: Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8795-800
references.pmid: 18550839
references.citation: Nat Genet. 2007 Apr;39(4):544-9
references.pmid: 17369828
references.citation: Plant Cell. 2005 Dec;17(12):3257-81
references.pmid: 16299223
references.citation: Annu Rev Plant Biol. 2006;57:675-709
references.pmid: 16669778
references.citation: Development. 2005 Sep;132(17):3787-98
references.pmid: 16100088
references.citation: Dev Cell. 2008 Oct;15(4):568-77
references.pmid: 18854141
references.citation: Dev Cell. 2005 Apr;8(4):517-27
references.pmid: 15809034
references.citation: Plant J. 2000 Feb;21(3):305-10
references.pmid: 10758481
references.citation: Front Endocrinol (Lausanne). 2012 Mar 28;3:45
references.pmid: 22645521
references.citation: Plant Cell. 2002;14 Suppl:S185-205
references.pmid: 12045277
references.citation: Plant J. 2010 Mar;61(6):1001-13
references.pmid: 20409274