title: From waste to plastic: synthesis of poly(3-hydroxypropionate) in Shimwellia blattae.
abstract: In recent years, glycerol has become an attractive carbon source for microbial processes, as it accumulates massively as a by-product of biodiesel production, also resulting in a decline of its price. A potential use of glycerol in biotechnology is the synthesis of poly(3-hydroxypropionate) [poly(3HP)], a biopolymer with promising properties which is not synthesized by any known wild-type organism. In this study, the genes for 1,3-propanediol dehydrogenase (dhaT) and aldehyde dehydrogenase (aldD) of Pseudomonas putida KT2442, propionate-coenzyme A (propionate-CoA) transferase (pct) of Clostridium propionicum X2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha H16 were cloned and expressed in the 1,3-propanediol producer Shimwellia blattae. In a two-step cultivation process, recombinant S. blattae cells accumulated up to 9.8% ± 0.4% (wt/wt [cell dry weight]) poly(3HP) with glycerol as the sole carbon source. Furthermore, the engineered strain tolerated the application of crude glycerol derived from biodiesel production, yielding a cell density of 4.05 g cell dry weight/liter in a 2-liter fed-batch fermentation process.
authors.affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms Universität Münster, Münster, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Heinrich
authors.forename: Björn
authors.initials: B
authors.lastname: Andreessen
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Madkour
authors.forename: Mansour A
authors.initials: MA
authors.lastname: Al-Ghamdi
authors.forename: Ibrahim I
authors.initials: II
authors.lastname: Shabbaj
authors.forename: Alexander
authors.initials: A
authors.lastname: Steinbüchel
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics; D000444:Aldehyde Dehydrogenase / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D060846:Cell Engineering / Q000379:methods; D004755:Enterobacteriaceae / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli; D005822:Genetic Vectors / Q000235:genetics; D005990:Glycerol / Q000378:metabolism*; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011108:Polymers; D016958:Pseudomonas putida / Q000201:enzymology
chemical_list: D011108:Polymers; D019344:Lactic Acid; C031601:hydracrylic acid; D000426:Alcohol Dehydrogenase; C052370:1,3-propanediol dehydrogenase; D000444:Aldehyde Dehydrogenase; D005990:Glycerol
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