title: Structure of glutaminyl cyclase from Drosophila melanogaster in space group I4.
abstract: The structure of ligand-free glutaminyl cyclase (QC) from Drosophila melanogaster (DmQC) has been determined in a novel crystal form. The protein crystallized in space group I4, with unit-cell parameters a = b = 122.3, c = 72.7 Å. The crystal diffracted to a resolution of 2 Å at the home source. The structure was solved by molecular replacement and was refined to an R factor of 0.169. DmQC exhibits a typical α/β-hydrolase fold. The electron density of three monosaccharides could be localized. The accessibility of the active site will facilitate structural studies of novel inhibitor-binding modes.
authors.affiliation: Department of Physical Biochemistry, Institute of Biochemistry and Biotechnology, MLU, Kurt-Mothes-Strasse 3, 06120 Halle, Germany. petr.kolenko@gmail.com
authors.forename: Petr
authors.initials: P
authors.lastname: Kolenko
authors.forename: Birgit
authors.initials: B
authors.lastname: Koch
authors.forename: Jens Ulrich
authors.initials: JU
authors.lastname: Rahfeld
authors.forename: Stephan
authors.initials: S
authors.lastname: Schilling
authors.forename: Hans Ulrich
authors.initials: HU
authors.lastname: Demuth
authors.forename: Milton T
authors.initials: MT
authors.lastname: Stubbs
mesh_terms: D019881:Aminoacyltransferases / Q000737:chemistry*; D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology*; D008958:Models, Molecular; D017434:Protein Structure, Tertiary
chemical_list: D019881:Aminoacyltransferases; C059280:glutaminyl-peptide cyclotransferase
keywords: Drosophila melanogaster; glutaminyl cyclase; soaking
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