title: Identification and characterization of a novel C20-elongase gene from the marine microalgae, Pavlova viridis, and its use for the reconstitution of two pathways of long-chain polyunsatured fatty acids biosynthesis in Saccharomyces cerevisiae.
abstract: The marine microalga, Pavlova viridis, contains long-chain polyunsatured fatty acids including eicosapentaenoic acid (EPA, 20:5n-3) and docosapentaenoic acid (DPA, 22:5n-3). A full-length cDNA sequence, pvelo5, was isolated from P. viridis. From sequence alignment, the gene was homologous to fatty acyl elongases from other organisms. Heterologous expression of pvelo5 in Saccharomyces cerevisiae confirmed that it encoded a specific C20-elongase within the n-3 and n-6 pathways. Elongation activity was confined exclusively to EPA and arachidonic acid (20:4n-6). GC analysis indicated that pvelo5 could co-express with other genes for biosynthesis to reconstitute the Δ8 and Δ6 pathways. Real-time PCR results and fatty acid analysis demonstrated that long-chain polyunsatured fatty acids production by the Δ8 pathway might be more effective than that by the Δ6 pathway.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin, 300071, China. bluelovely123@yahoo.com.cn
authors.forename: Tonglei
authors.initials: T
authors.lastname: Shi
authors.forename: Aiqun
authors.initials: A
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000096:biosynthesis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D015870:Gene Expression; D058087:Haptophyta / Q000201:enzymology* / Q000235:genetics; D060847:Metabolic Engineering / Q000379:methods; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D048028:DNA, Algal; D005227:Fatty Acids; D011994:Recombinant Proteins; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases