title: In vivo detection of human TRPV6-rich tumors with anti-cancer peptides derived from soricidin.
abstract: Soricidin is a 54-amino acid peptide found in the paralytic venom of the northern short-tailed shrew (Blarina brevicauda) and has been found to inhibit the transient receptor potential of vallinoid type 6 (TRPV6) calcium channels. We report that two shorter peptides, SOR-C13 and SOR-C27, derived from the C-terminus of soricidin, are high-affinity antagonists of human TRPV6 channels that are up-regulated in a number of cancers. Herein, we report molecular imaging methods that demonstrate the in vivo diagnostic potential of SOR-C13 and SOR-C27 to target tumor sites in mice bearing ovarian or prostate tumors. Our results suggest that these novel peptides may provide an avenue to deliver diagnostic and therapeutic reagents directly to TRPV6-rich tumors and, as such, have potential applications for a range of carcinomas including ovarian, breast, thyroid, prostate and colon, as well as certain leukemia's and lymphomas.
authors.affiliation: National Research Council of Canada, Institute for Biodiagnostics (Atlantic)-Neuroimaging Research Laboratory, Halifax, Nova Scotia, Canada.
authors.forename: Chris V
authors.initials: CV
authors.lastname: Bowen
authors.forename: Drew
authors.initials: D
authors.lastname: DeBay
authors.forename: H Stephen
authors.initials: HS
authors.lastname: Ewart
authors.forename: Pamela
authors.initials: P
authors.lastname: Gallant
authors.forename: Sean
authors.initials: S
authors.lastname: Gormley
authors.forename: T Toney
authors.initials: TT
authors.lastname: Ilenchuk
authors.forename: Umar
authors.initials: U
authors.lastname: Iqbal
authors.forename: Tyler
authors.initials: T
authors.lastname: Lutes
authors.forename: Marzia
authors.initials: M
authors.lastname: Martina
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Mealing
authors.forename: Nadine
authors.initials: N
authors.lastname: Merkley
authors.forename: Sandra
authors.initials: S
authors.lastname: Sperker
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Moreno
authors.forename: Christopher
authors.initials: C
authors.lastname: Rice
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Syvitski
authors.forename: John M
authors.initials: JM
authors.lastname: Stewart
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D005456:Fluorescent Dyes; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008968:Molecular Conformation; D057054:Molecular Imaging; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D061848:Optical Imaging; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014183:Transplantation, Heterologous
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D050916:TRPV Cation Channels; C120776:TRPV6 channel
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