title: Immunomodulatory effects of Escherichia coli ATCC 25922 on allergic airway inflammation in a mouse model.
abstract: BACKGROUND
  Hygiene hypothesis demonstrates that the lack of microbial exposure would promote the development of allergic airway disease (AAD). Therefore, the gut microbiota, including Escherichia coli (E. coli), would probably offer a potential strategy for AAD.
  
  
  OBJECTIVE
  To investigate whether E. coli infection is able to suppress the induction of AAD and to elucidate the underlying mechanisms.
  
  
  METHODS
  Nonpathogenic E. coli ATCC 25922 was infected by gavage before AAD phase in three patterns: 10(8) or 10(6) CFU in neonates or 10(8) CFU in adults. Then mice were sensitized and challenged with ovalbumin (OVA) to induce allergic inflammation in both the upper and lower airways. Hallmarks of AAD, in terms of eosinophil infiltration and goblet cell metaplasia in subepithelial mucosa, Th2 skewing of the immune response, and levels of T regulate cells (Tregs), were examined by histological analysis, ELISA, and flow cytometry, respectively.
  
  
  RESULTS
  E. coli, especially neonatally infected with an optimal dose, attenuated allergic responses, including a decrease in nasal rubbing and sneezing, a reduction in eosinophil inflammation and goblet cell metaplasia in subepithelial mucosa, decreased serum levels of OVA-specific IgE, and reduced Th2 (IL-4) cytokines. In contrast, this effect came with an increase of Th1 (IFN-r and IL-2) cytokines, and an enhancement of IL-10-secreting Tregs in paratracheal lymph nodes (PTLN).
  
  
  CONCLUSIONS
  E. coli suppresses allergic responses in mice, probably via a shift from Th1 to Th2 and/or induction of Tregs. Moreover, this infection is age- and dose-dependent, which may open up novel possibilities for new therapeutic interventions.
authors.affiliation: Institute of Eye and Otorhinolaryngology, Department of Otorhinolaryngology-Head and Neck Surgery, Provincial Hospital affiliated to Shandong University, Jinan, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Pang
authors.forename: Hefeng
authors.initials: H
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Yueqi
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Wang
authors.forename: Mingming
authors.initials: M
authors.lastname: Wang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Guanggang
authors.initials: G
authors.lastname: Shi
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D016535:Bronchial Hyperreactivity / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D056747:Immunomodulation; D007249:Inflammation / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010047:Ovalbumin / Q000009:adverse effects / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E; D010047:Ovalbumin
references.citation: Science. 2002 Apr 19;296(5567):490-4
references.pmid: 11964470
references.citation: BMC Complement Altern Med. 2011 Jun 03;11:45
references.pmid: 21639872
references.citation: J Allergy Clin Immunol. 2011 Mar;127(3):701-21.e1-70
references.pmid: 21377040
references.citation: Allergy. 2008 Apr;63 Suppl 86:8-160
references.pmid: 18331513
references.citation: Nat Rev Immunol. 2011 Dec 09;12(1):9-23
references.pmid: 22158411
references.citation: Am J Rhinol. 2000 Sep-Oct;14(5):325-33
references.pmid: 11068658
references.citation: Am J Respir Crit Care Med. 2009 May 15;179(10):875-82
references.pmid: 19234107
references.citation: Allergy. 1998;53(46 Suppl):20-5
references.pmid: 9825991
references.citation: Eur J Pharmacol. 2008 May 13;585(2-3):354-60
references.pmid: 18410921
references.citation: Stem Cells. 2012 Dec;30(12):2692-9
references.pmid: 22987325
references.citation: Science. 2012 Jun 8;336(6086):1268-73
references.pmid: 22674334
references.citation: Aliment Pharmacol Ther. 2003 Jul 1;18(1):45-56
references.pmid: 12848625
references.citation: N Engl J Med. 2011 Feb 24;364(8):701-9
references.pmid: 21345099
references.citation: J Bacteriol. 1992 Aug;174(15):5057-62
references.pmid: 1629162
references.citation: Am J Clin Nutr. 1999 May;69(5):1035S-1045S
references.pmid: 10232646
references.citation: Nat Med. 2005 Apr;11(4):446-9
references.pmid: 15793580
references.citation: Int Immunopharmacol. 2008 Aug;8(8):1083-8
references.pmid: 18550011
references.citation: Nat Med. 2012 Mar 25;18(4):538-46
references.pmid: 22447074
references.citation: Expert Rev Clin Immunol. 2010 May;6(3):413-23
references.pmid: 20441427
references.citation: Science. 1997 Jan 3;275(5296):77-9
references.pmid: 8974396
references.citation: Clin Exp Allergy. 2012 Mar;42(3):405-11
references.pmid: 22093109
references.citation: Clin Exp Allergy. 2009 Sep;39(9):1314-23
references.pmid: 19538496
references.citation: Nat Rev Microbiol. 2008 Oct;6(10):776-88
references.pmid: 18794915
references.citation: Clin Exp Allergy. 2003 Aug;33(8):1083-9
references.pmid: 12911782
references.citation: PLoS One. 2011;6(7):e22004
references.pmid: 21789200
references.citation: Clin Exp Allergy. 2001 May;31(5):782-90
references.pmid: 11422139
references.citation: N Engl J Med. 2006 Nov 23;355(21):2226-35
references.pmid: 17124020
references.citation: J Clin Invest. 2011 Aug;121(8):3088-93
references.pmid: 21737881
references.citation: Stem Cells. 2009 Jan;27(1):259-65
references.pmid: 18832595
references.citation: J Food Prot. 2009 May;72(5):937-44
references.pmid: 19517718
references.citation: J Allergy Clin Immunol. 2006 May;117(5):969-77; quiz 978
references.pmid: 16675321
references.citation: Nature. 2008 Jul 24;454(7203):445-54
references.pmid: 18650915
references.citation: Microbiol Mol Biol Rev. 2010 Sep;74(3):453-76
references.pmid: 20805407
references.citation: Clin Exp Immunol. 2010 Apr;160(1):1-9
references.pmid: 20415844
references.citation: EMBO Rep. 2012 May 01;13(5):440-7
references.pmid: 22422004
references.citation: BMJ. 1989 Nov 18;299(6710):1259-60
references.pmid: 2513902
references.citation: J Antimicrob Chemother. 1992 Feb;29(2):121-7
references.pmid: 1506326
references.citation: Science. 2012 Apr 27;336(6080):489-93
references.pmid: 22442383
references.citation: J Allergy Clin Immunol. 2005 Aug;116(2):332-40
references.pmid: 16083788
references.citation: Methods Mol Med. 2007;136:87-96
references.pmid: 17983142
references.citation: J Allergy Clin Immunol. 2010 Sep;126(3):466-76
references.pmid: 20816182
references.citation: Acta Microbiol Immunol Hung. 1994;41(2):215-9
references.pmid: 7804725
references.citation: Nat Rev Immunol. 2012 Apr 13;12(5):319
references.pmid: 22498779
references.citation: Nat Rev Microbiol. 2009 Dec;7(12):887-94
references.pmid: 19898491
references.citation: Ann Agric Environ Med. 2012;19(3):475-81
references.pmid: 23020042
references.citation: Nat Rev Immunol. 2001 Nov;1(2):108-16
references.pmid: 11905818
references.citation: Thorax. 2010 Dec;65(12):1053-60
references.pmid: 20965927