title: The δ subunit of RNA polymerase is required for rapid changes in gene expression and competitive fitness of the cell.
abstract: RNA polymerase (RNAP) is an extensively studied multisubunit enzyme required for transcription of DNA into RNA, yet the δ subunit of RNAP remains an enigmatic protein whose physiological roles have not been fully elucidated. Here, we identify a novel, so far unrecognized function of δ from Bacillus subtilis. We demonstrate that δ affects the regulation of RNAP by the concentration of the initiating nucleoside triphosphate ([iNTP]), an important mechanism crucial for rapid changes in gene expression in response to environmental changes. Consequently, we demonstrate that δ is essential for cell survival when facing a competing strain in a changing environment. Hence, although δ is not essential per se, it is vital for the cell's ability to rapidly adapt and survive in nature. Finally, we show that two other proteins, GreA and YdeB, previously implicated to affect regulation of RNAP by [iNTP] in other organisms, do not have this function in B. subtilis.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Alžbeta
authors.initials: A
authors.lastname: Rabatinová
authors.forename: Hana
authors.initials: H
authors.lastname: Šanderová
authors.forename: Jitka
authors.initials: J
authors.lastname: Jirát Matějčková
authors.forename: Jana
authors.initials: J
authors.lastname: Korelusová
authors.forename: Luděk
authors.initials: L
authors.lastname: Sojka
authors.forename: Ivan
authors.initials: I
authors.lastname: Barvík
authors.forename: Veronika
authors.initials: V
authors.lastname: Papoušková
authors.forename: Vladimír
authors.initials: V
authors.lastname: Sklenár
authors.forename: Lukáš
authors.initials: L
authors.lastname: Žídek
authors.forename: Libor
authors.initials: L
authors.lastname: Krásný
mesh_terms: D000222:Adaptation, Physiological; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D055786:Gene Knockout Techniques; D050296:Microbial Viability; D011401:Promoter Regions, Genetic / Q000235:genetics; D021122:Protein Subunits; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D021122:Protein Subunits; D012321:DNA-Directed RNA Polymerases
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