title: Metre-long cell-laden microfibres exhibit tissue morphologies and functions.
abstract: Artificial reconstruction of fibre-shaped cellular constructs could greatly contribute to tissue assembly in vitro. Here we show that, by using a microfluidic device with double-coaxial laminar flow, metre-long core-shell hydrogel microfibres encapsulating ECM proteins and differentiated cells or somatic stem cells can be fabricated, and that the microfibres reconstitute intrinsic morphologies and functions of living tissues. We also show that these functional fibres can be assembled, by weaving and reeling, into macroscopic cellular structures with various spatial patterns. Moreover, fibres encapsulating primary pancreatic islet cells and transplanted through a microcatheter into the subrenal capsular space of diabetic mice normalized blood glucose concentrations for about two weeks. These microfibres may find use as templates for the reconstruction of fibre-shaped functional tissues that mimic muscle fibres, blood vessels or nerve networks in vivo.
authors.affiliation: Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onoe
authors.forename: Teru
authors.initials: T
authors.lastname: Okitsu
authors.forename: Akane
authors.initials: A
authors.lastname: Itou
authors.forename: Midori
authors.initials: M
authors.lastname: Kato-Negishi
authors.forename: Riho
authors.initials: R
authors.lastname: Gojo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kiriya
authors.forename: Koji
authors.initials: K
authors.lastname: Sato
authors.forename: Shigenori
authors.initials: S
authors.lastname: Miura
authors.forename: Shintaroh
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Kaori
authors.initials: K
authors.lastname: Kuribayashi-Shigetomi
authors.forename: Yukiko T
authors.initials: YT
authors.lastname: Matsunaga
authors.forename: Yuto
authors.initials: Y
authors.lastname: Shimoyama
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D000464:Alginates; D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D005109:Extracellular Matrix; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D032342:Muscle Cells / Q000166:cytology; D032383:Myocytes, Cardiac; D041681:NIH 3T3 Cells; D051381:Rats; D023822:Tissue Engineering / Q000379:methods
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
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