title: The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome.
abstract: Decoding of the AUA isoleucine codon in bacteria and archaea requires modification of a C in the anticodon wobble position of the isoleucine tRNA. Here, we report the crystal structure of the archaeal tRNA2(Ile), which contains the modification agmatidine in its anticodon, in complex with the AUA codon on the 70S ribosome. The structure illustrates how agmatidine confers codon specificity for AUA over AUG.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Voorhees
authors.forename: Debabrata
authors.initials: D
authors.lastname: Mandal
authors.forename: Cajetan
authors.initials: C
authors.lastname: Neubauer
authors.forename: Caroline
authors.initials: C
authors.lastname: Köhrer
authors.forename: Uttam L
authors.initials: UL
authors.lastname: RajBhandary
authors.forename: V
authors.initials: V
authors.lastname: Ramakrishnan
mesh_terms: D001105:Archaea / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003062:Codon; D007532:Isoleucine / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012355:RNA, Transfer, Ile / Q000737:chemistry* / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003062:Codon; D012355:RNA, Transfer, Ile; D007532:Isoleucine
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