title: Triterpenoid saponins from the roots of Gypsophila trichotoma Wender.
abstract: Eleven triterpenoid saponins were isolated from the roots of Gypsophila trichotoma Wender. (G. trichotoma Wender. var. trichotoma) (Caryophyllaceae), together with one known compound. The structures were established on the basis of extensive NMR analysis ((1)H, (13)C NMR, COSY, TOCSY, ROESY, HSQC, and HMBC), completed by analysis of HR-ESI-MS and ESI-MS(n). The saponins have the commonly found gypsogenin as the aglycone substituted at C-3 with trisaccharide and at C-28 with oligosaccharide through a fucose residue, as saponins isolated from Gypsophila perfoliata L. originated from China. The oligosaccharide attached to C-28 is substituted with acetyl and (or) sulfate groups. Тhe cytotoxicity of the saponin extract from G. trichotoma was evaluated against a rat alveolar macrophage-like cell line NR8383 and human leukemia cell lines U937 and BV-173. The synergistic effect of the aminoacyl saponins, previously isolated from G. trichotoma, was tested for its ability to enhance the cytotoxicity of the targeted toxin in HER14 cells.
authors.affiliation: Institute of Molecular Chemistry of Reims, UMR CNRS 7312, Bâtiment 18, BP 1039, 51687 Reims cedex 2, France. Laurence.Voutquenne@univ-reims.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Voutquenne-Nazabadioko
authors.forename: Reneta
authors.initials: R
authors.lastname: Gevrenova
authors.forename: Nicolas
authors.initials: N
authors.lastname: Borie
authors.forename: Dominique
authors.initials: D
authors.lastname: Harakat
authors.forename: Charlotte
authors.initials: C
authors.lastname: Sayagh
authors.forename: Alexander
authors.initials: A
authors.lastname: Weng
authors.forename: Mayank
authors.initials: M
authors.lastname: Thakur
authors.forename: Maya
authors.initials: M
authors.lastname: Zaharieva
authors.forename: Max
authors.initials: M
authors.lastname: Henry
mesh_terms: D000818:Animals; D029748:Caryophyllaceae / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006801:Humans; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D012503:Saponins; D014315:Triterpenes