title: Resveratrol prevents interleukin-1β-induced dysfunction of pancreatic β-cells.
abstract: OBJECTIVE
  Interleukin-1β (IL-1β) plays an important role in the development of type 1 and type 2 diabetes mellitus. Resveratrol, a polyphenol, is known to have a wide range of pharmacological properties in vitro. In this research, we examined the effects of resveratrol on IL-1β-induced β-cell dysfunction.
  
  
  METHODS
  We first evaluated the effect of resveratrol on nitric oxide (NO) formation in RINm5F cells stimulated with IL-1β using the Griess method. Next, we performed transient transfection and reporter assays to measure the transcriptional activity of peroxisome proliferator-activated receptor-γ (PPAR-γ). We also used Western blotting analysis to assess the effect of resveratrol on inducible nitric oxide synthase (iNOS) expression and nuclear factor-κB (NF-κB) translocation to the nuclei in cells treated with IL-1β. In addition, we assessed the transcriptional activity of NF-κB using an electrophoretic mobility shift assay (EMSA). Finally, we evaluated the effect of resveratrol on IL-1β-induced inhibition of glucose-stimulated insulin secretion in freshly isolated rat pancreatic islets.
  
  
  RESULTS
  Resveratrol significantly suppressed IL-1β-induced NO production, a finding that correlated well with reduced levels of iNOS mRNA and protein. The molecular mechanism by which resveratrol inhibited iNOS gene expression appeared to involve increased PPAR-γ activity, which resulted in the inhibition of NF-κB activation. Further analysis showed that resveratrol could prevent IL-1β-induced inhibition of glucose-stimulated insulin secretion in rat islets.
  
  
  CONCLUSIONS
  In this study, we demonstrated that resveratrol could protect against pancreatic β-cell dysfunction caused by IL-1β.
authors.affiliation: Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.forename: Changwen
authors.initials: C
authors.lastname: Jing
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ji
authors.forename: Yujie
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
keywords: interleukin-1β; nitric oxide; nuclear factor-κB.; peroxisome proliferator-activated receptor-γ; resveratrol
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