title: Separation and purification of the antioxidant compounds, caffeic acid phenethyl ester and caffeic acid from mushrooms by molecularly imprinted polymer.
abstract: Caffeic acid phenethyl ester (CAPE) and caffeic acid (CA), two naturally occurring phenolic antioxidants, have been reported to have a diversity of biological activities. In this investigation, a novel approach to separate and enrich CAPE and CA from 25 species of mushrooms using molecularly imprinted polymers (MIPs) as the sorbent material is reported. The MIPs were synthesized using CAPE as the template, and its adsorption behavior was investigated in detail. In comparison with C18-solid phase extraction (SPE), MIP-SPE displayed high selectivity and good affinity for CAPE and CA. The antioxidant potential of the mushroom extracts, before and after preconcentration using MIPs, was assayed by inhibition of erythrocyte hemolysis and lipid peroxidation. Application of MIPs with a high affinity toward CAPE and CA provides a novel method for obtaining active compounds from natural products.
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. ln031101@yahoo.com.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.forename: Jack Ho
authors.initials: JH
authors.lastname: Wong
authors.forename: Ji Xuan
authors.initials: JX
authors.lastname: Qiao
authors.forename: Ye Ni
authors.initials: YN
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.forename: Rong Rong
authors.initials: RR
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification*; D054802:Molecular Imprinting; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D011108:Polymers; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol