title: Separation of nonfucosylated antibodies with immobilized FcγRIII receptors.
abstract: Post-translational modifications can dramatically impact protein activity, but identifying such structure:function relationships, as well as capitalizing on functionally enhanced variants, is a significant challenge. Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity.
authors.affiliation: Process Biochemistry, Biogen Idec, Cambridge, MA, 02142, USA.
authors.forename: Glen R
authors.initials: GR
authors.lastname: Bolton
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Ackerman
authors.forename: Austin W
authors.initials: AW
authors.lastname: Boesch
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016466:CHO Cells; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D005643:Fucose; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D017452:Receptors, IgG / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D055767:Immobilized Proteins; D017452:Receptors, IgG; D005643:Fucose