title: Rapid characterization of wheat low molecular weight glutenin subunits by ultraperformance liquid chromatography (UPLC).
abstract: Low molecular weight glutenin subunits (LMW-GS), as important seed storage proteins, together with HMW-GS significantly define unique dough viscoelastic properties. In this study, a rapid ultraperformance liquid chromatography (UPLC) method for the separation and characterization of LMW-GS in wheat was optimized and established. The fast, reproducible, and high-resolution UPLC separation of LMW-GS could be obtained by gradually increasing eluting gradient from 21 to 47% in 30 min at flow rate of 0.55 mL/min and 60 °C for separation temperature. By this method, analysis of one sample could be completed in <20 min, significantly less time than the traditional reversed-phase high-performance liquid chromatography (RP-HPLC) method. Under the optimized conditions, the genetic features of LMW-GS and genotype × environmental interaction were successfully analyzed, leading to a fast identification of 17 main LMW-GS alleles that were related to different quality properties in wheat. The results demonstrated that UPLC could be a powerful and alternative tool for genetic and proteomic studies of wheat grain proteins and fast identification or screening of desirable LMW-GS alleles in wheat quality improvement.
authors.affiliation: College of Life Science, Capital Normal University , 100048 Beijing, China.
authors.forename: Zitong
authors.initials: Z
authors.lastname: Yu
authors.forename: Caixia
authors.initials: C
authors.lastname: Han
authors.forename: Xing
authors.initials: X
authors.lastname: Yan
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Guoliang
authors.initials: G
authors.lastname: Jiang
authors.forename: Yueming
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000483:Alleles; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000737:chemistry* / Q000235:genetics; D008970:Molecular Weight; D040901:Proteomics; D014908:Triticum / Q000737:chemistry* / Q000235:genetics
chemical_list: D005983:Glutens; C037302:glutenin