title: C/EBPα is required for development of dendritic cell progenitors.
abstract: Dendritic cells (DCs) are master regulators of the immune system, but molecular regulation of early DC differentiation has been poorly understood. Here, we report that the transcription factor C/EBPα coordinates the development of progenitor cells required for production of multiple categories of DCs. C/EBPα was needed for differentiation from stem/progenitor cells to common DC progenitors (CDPs), but not for transition of CDP to mature DCs. C/EBPα deletion in mature DCs did not affect their numbers or function, suggesting that this transcription factor is not needed for maintenance of DCs in lymphoid tissues. ChIP-seq and microarrays were used to identify candidate genes regulated by C/EBPα and required for DC formation. Genes previously shown to be critical for DC formation were bound by C/EBPα, and their expression was decreased in the earliest hematopoietic compartments in the absence of C/EBPα. These data indicate that C/EBPα is important for the earliest stages of steady-state DC differentiation.
authors.affiliation: Harvard Stem Cell Institute, Harvard Medical School, Boston, MA, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Welner
authors.forename: Deepak
authors.initials: D
authors.lastname: Bararia
authors.forename: Giovanni
authors.initials: G
authors.lastname: Amabile
authors.forename: Akos
authors.initials: A
authors.lastname: Czibere
authors.forename: Touati
authors.initials: T
authors.lastname: Benoukraf
authors.forename: Christian
authors.initials: C
authors.lastname: Bach
authors.forename: K Disnika Senali Abayratna
authors.initials: KD
authors.lastname: Wansa
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Tadafumi
authors.initials: T
authors.lastname: Iino
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hetherington
authors.forename: Koichi
authors.initials: K
authors.lastname: Akashi
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Tenen
mesh_terms: D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D016000:Cluster Analysis; D003713:Dendritic Cells / Q000378:metabolism / Q000502:physiology*; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha
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