title: Multifunctional hybrid silica nanoparticles for controlled doxorubicin loading and release with thermal and pH dually response.
abstract: Controlled drug loading and release into tumor cells to increase the intracellular drug concentration is a major challenge for cancer therapy due to resistance and inefficient cellular uptake. Here a temperature and pH dually responsive PNiPAM/AA@SiO2core-shell particles with internal controlled release were designed and fabricated for efficient cancer treatment, which could recognize the intrinsic pH differences between cancers and normal tissues. Upon lowering the temperature, doxorubicin was loaded into the PNiPAM/AA@SiO2nanoparticles, whereas by increasing the acidity, previously loaded doxorubicin was quickly released. Comparing with common mesoporous silica particles (MSNs), this core-shell particle has more uniform size and better dispersity. In addition, dried PNiPAM/AA@SiO2nanoparticles could be easily redispersed in distilled water. The in vitro cell culture experiments showed that not only PNiPAM/AA@SiO2particles were more biocompatible and lower cytotoxic than MSN, but also DOX@PNiPAM/AA@SiO2had higher drug releasing efficiency in the lysosomes and stronger inhibitory effect on tumor cell growth than DOX@MSN. All these features indicated that PNiPAM/AA@SiO2particles have great potential in therapy applications.
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and nanosafety, National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Xixue
authors.initials: X
authors.lastname: Hu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Hao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Jinchao
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Zhang
authors.forename: Paul C
authors.initials: PC
authors.lastname: Wang
authors.forename: Guozhang
authors.initials: G
authors.lastname: Zou
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Liang
references.citation: Langmuir. 2006 Jul 4;22(14):5974-8
references.pmid: 16800645
references.citation: ACS Nano. 2010 Sep 28;4(9):5301-13
references.pmid: 20698555
references.citation: Int J Pharm. 2011 Dec 15;421(2):388-96
references.pmid: 22001840
references.citation: Adv Drug Deliv Rev. 2006 Dec 30;58(15):1655-70
references.pmid: 17125884
references.citation: Angew Chem Int Ed Engl. 2011 Jan 17;50(3):640-3
references.pmid: 21226142
references.citation: J Control Release. 2007 Oct 18;123(1):19-26
references.pmid: 17826863
references.citation: Angew Chem Int Ed Engl. 2007;46(40):7548-58
references.pmid: 17854012
references.citation: J Am Chem Soc. 2003 Apr 16;125(15):4451-9
references.pmid: 12683815
references.citation: J Control Release. 2007 Feb 26;117(3):396-402
references.pmid: 17239981
references.citation: Int J Pharm. 2011 Jan 17;403(1-2):139-61
references.pmid: 20951779
references.citation: Adv Drug Deliv Rev. 2001 Dec 31;53(3):321-39
references.pmid: 11744175
references.citation: Adv Healthc Mater. 2012 Sep;1(5):567-72
references.pmid: 23184791
references.citation: Nano Lett. 2005 Oct;5(10):1987-91
references.pmid: 16218723
references.citation: J Control Release. 2004 Jul 7;97(3):513-25
references.pmid: 15212883
references.citation: Phys Chem Chem Phys. 2011 Jun 7;13(21):9982-5
references.pmid: 21739680
references.citation: J Colloid Interface Sci. 2002 Jun 15;250(2):484-91
references.pmid: 16290689
references.citation: J Control Release. 1998 Oct 30;55(1):87-98
references.pmid: 9795019
references.citation: J Am Chem Soc. 2010 Sep 22;132(37):13016-25
references.pmid: 20799689
references.citation: J Biomed Nanotechnol. 2011 Feb;7(1):60-2
references.pmid: 21485803
references.citation: Nat Mater. 2011 May;10(5):389-97
references.pmid: 21499315
references.citation: Angew Chem Int Ed Engl. 2011 Sep 12;50(38):8853-7
references.pmid: 21826770
references.citation: J Am Chem Soc. 2010 Sep 15;132(36):12690-7
references.pmid: 20718462
references.citation: Small. 2010 Aug 16;6(16):1794-805
references.pmid: 20623530
references.citation: Acc Chem Res. 2007 Sep;40(9):846-53
references.pmid: 17645305
references.citation: J Am Chem Soc. 2011 Dec 14;133(49):19582-5
references.pmid: 21981330
references.citation: ACS Nano. 2008 May;2(5):889-96
references.pmid: 19206485
references.citation: Eur J Pharm Biopharm. 2007 Mar;65(3):379-87
references.pmid: 17123802
references.citation: J Am Chem Soc. 2011 Dec 21;133(50):20444-57
references.pmid: 22050408
references.citation: Angew Chem Int Ed Engl. 2010 Oct 25;49(44):8214-9
references.pmid: 20865709
references.citation: Nanoscale. 2012 Jun 7;4(11):3577-83
references.pmid: 22543578
references.citation: J Control Release. 1999 Nov 1;62(1-2):115-27
references.pmid: 10518643
references.citation: Int J Hyperthermia. 1995 Mar-Apr;11(2):211-6
references.pmid: 7790735
references.citation: Nano Lett. 2006 Apr;6(4):662-8
references.pmid: 16608261