title: Variants of the arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene and risk of ischemic stroke in Han Chinese of eastern China.
abstract: Variants of the arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene have been suggested to play an important role in the pathogenesis of atherosclerosis and ischemic stroke. This study was aimed to explore the association of ALOX5AP variants with ischemic stroke risk in Han Chinese of eastern China. A total of 690 ischemic stroke cases and 767 controls were recruited. The subjects were further subtyped according to the Trial of Org 10172 in Acute Stroke Treatment (TOAST) criteria. On the basis of that, two polymorphisms of the ALOX5AP gene (rs10507391 and rs12429692) were determined by TaqMan genotyping assay. In addition, plasma leukotriene B4 (LTB4) levels were analyzed in these subjects. There was no evidence of association between the two variants of ALOX5AP and the risk of ischemic stroke or its TOAST-subtypes. Haplotype analysis and stratification analysis according to sex, age, body mass index, hypertension, and diabetes also showed negative association. Analysis of LTB4 levels in a subset of cases and controls revealed that LTB4 levels were significantly higher in ischemic stroke cases than in the controls (70.06±14.75 ng/L vs 57.34±10.93 ng/L; P = 0.000) and carriers of the T allele of the rs10507391 variant were associated with higher plasma LTB4 levels (P = 0.000). The present study suggests there is no association of the two polymorphisms in the ALOX5AP gene with ischemic stroke risk in Han Chinese of eastern China.
authors.affiliation: Emergency Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors.forename: Gannan
authors.initials: G
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.forename: Weijuan
authors.initials: W
authors.lastname: Cao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhang
keywords: arachidonate 5-lipoxygenase-activating protein; ischemic stroke; leukotriene B4; risk factors; variants
references.citation: Yi Chuan Xue Bao. 2006 Aug;33(8):678-84
references.pmid: 16939001
references.citation: Cerebrovasc Dis. 2010;29(6):528-37
references.pmid: 20357438
references.citation: Am J Hum Genet. 2005 Mar;76(3):505-9
references.pmid: 15640973
references.citation: Am J Epidemiol. 2009 Mar 1;169(5):523-32
references.pmid: 19126581
references.citation: Eur J Hum Genet. 2009 Nov;17(11):1448-53
references.pmid: 19417766
references.citation: Stroke. 1993 Jan;24(1):35-41
references.pmid: 7678184
references.citation: Trends Pharmacol Sci. 2008 Feb;29(2):72-8
references.pmid: 18187210
references.citation: Curr Pharm Des. 2008;14(33):3590-600
references.pmid: 19075735
references.citation: Int J Mol Med. 2008 Jun;21(6):801-8
references.pmid: 18506375
references.citation: Stroke. 2006 Feb;37(2):288-90
references.pmid: 16410477
references.citation: Stroke. 2003 Sep;34(9):2091-6
references.pmid: 12907817
references.citation: Eur J Hum Genet. 2008 Sep;16(9):1117-25
references.pmid: 18398440
references.citation: J Neurol Sci. 2007 Dec 15;263(1-2):113-7
references.pmid: 17655870
references.citation: Clin Exp Pharmacol Physiol. 2010 Feb;37(2):259-64
references.pmid: 19769611
references.citation: Curr Drug Targets. 2010 Jul;11(7):882-7
references.pmid: 20388065
references.citation: Curr Pharm Des. 2009;15(27):3116-32
references.pmid: 19754386
references.citation: Ann Neurol. 2005 Sep;58(3):351-61
references.pmid: 16130105
references.citation: J Intern Med. 2010 Feb;267(2):139-55
references.pmid: 20175863
references.citation: Ann Hum Genet. 2007 Sep;71(Pt 5):620-9
references.pmid: 17521309
references.citation: Hum Genet. 2007 Jun;121(5):601-7
references.pmid: 17387518
references.citation: Atherosclerosis. 2009 Jun;204(2):e58-63
references.pmid: 19046748
references.citation: Mol Biol Rep. 2011 Oct;38(7):4731-8
references.pmid: 21153769
references.citation: Stroke. 2005 Apr;36(4):731-6
references.pmid: 15731479
references.citation: Trends Mol Med. 2005 May;11(5):217-24
references.pmid: 15882609
references.citation: Stroke. 2009 Nov;40(11):3436-42
references.pmid: 19729601
references.citation: Stroke. 2003 Oct;34(10):2518-32
references.pmid: 14500942
references.citation: Stroke. 2008 Jun;39(6):1688-93
references.pmid: 18323480
references.citation: Nat Genet. 2004 Mar;36(3):233-9
references.pmid: 14770184
references.citation: Stroke. 2008 Apr;39(4):1109-14
references.pmid: 18323512
references.citation: Stroke. 2006 Aug;37(8):2007-11
references.pmid: 16778124
references.citation: Am J Hum Genet. 2003 Nov;73(5):1162-9
references.pmid: 14574645