title: Effects of vitamin E on expressions of eight microRNAs in the liver of Nile tilapia (Oreochromis niloticus).
abstract: Currently, microRNAs (miRNAs) are known to regulate cellular processes such as apoptosis, differentiation, cell cycle, and immune functions, and their expression can be altered by distinct stress conditions, such as oxidative stress. In immune systems of fish, vitamin E (VE) has a defined role as an antioxidant. In order to understand the molecular mechanism of vitamin E defending from oxidative stress, three groups of juvenile Nile tilapia (Oreochromis niloticus) (initial weight 3.25 ± 0.02 g) were fed to satiation with 3 semi-purified diets containing VE (DL-α-tocopherol acetate) of 0, 50, and 2500 mg/kg supplementation, respectively, with the expressions of eight miRNAs (miR-21, miR-223, miR-146a, miR-125b, miR-181a, miR-16, miR-155 and miR-122) in the liver of tilapia subsequently detected after 8-week growth experiment. Results showed that VE-deficient (0 mg/kg supplementation) decreased the activity of superoxide dismutase (SOD), and decreased the expressions of miR-223, miR-146a, miR-16 and miR-122, while excessive supplementation of VE (2500 mg/kg) decreased SOD activity and increased the expressions of all the eight miRNAs. The targets of the eight miRNAs were further predicated with bioinformatic approach and the possible regulating mechanisms of VE via miRNAs were analyzed. The present study confirmed that the differences in dietary VE affected expression of hepatic miRNAs which may partly demonstrate the molecular mechanism of VE, and the new idea of introducing miRNAs into research will provide the basic data for researches of molecular nutrition.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Tang
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Xu
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Pan
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Fu
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D023681:Cichlids / Q000235:genetics* / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D035683:MicroRNAs; D014810:Vitamin E; D013482:Superoxide Dismutase