title: Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation.
abstract: A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 10(8) cells) is achieved.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
authors.forename: Mado
authors.initials: M
authors.lastname: Miyahara
authors.forename: Masaki
authors.initials: M
authors.lastname: Aoi
authors.forename: Natsuko
authors.initials: N
authors.lastname: Inoue-Kashino
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kashino
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ifuku
mesh_terms: D017377:Diatoms / Q000166:cytology / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D030781:Organisms, Genetically Modified; D014170:Transformation, Genetic