title: Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o, histone acetyltransferase 1, and deacetylase 1 in metaphase II mouse oocytes.
abstract: OBJECTIVE
  To investigate the effect of vitrification on Dnmt1o, Hat1, and Hdac1 promoter CpG island methylation patterns and messenger RNA (mRNA) expression levels in mouse metaphase II (MII) oocytes.
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  Academic institution.
  
  
  METHODS
  Kunming white mice.
  
  
  METHODS
  After vitrification, surviving mouse MII oocytes subjected to methylation and expression analysis with fresh oocytes used as a control.
  
  
  METHODS
  Expression levels of mRNA as measured by real-time reverse-transcriptase polymerase chain reaction of methylation patterns of the CpG islands in the Dnmt1o, Hat1, and Hdac1 promoters analyzed by bisulfite mutagenesis and sequencing.
  
  
  RESULTS
  The methylation patterns of the promoter CpG islands in Dnmt1o, Hat1, and Hdac1 were not statistically significantly when comparing vitrified oocytes and fresh oocytes. The expression levels of Hat1 and Hdac1 mRNA were not statistically significantly different in comparing in vitrified oocytes and fresh oocytes. The expression of Dnmt1o mRNA was statistically significantly lower in vitrified oocytes compared with fresh oocytes.
  
  
  CONCLUSIONS
  Vitrification did not statistically significantly alter the methylation patterns of the promoter CpG islands in Dnmt1o, Hat1, or Hdac1, but did statistically significantly decrease the expression of Dnmt1o mRNA in mouse MII oocytes.
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Zhao
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Ren
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Du
authors.forename: Hai-Sheng
authors.initials: HS
authors.lastname: Hao
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Tong
authors.initials: T
authors.lastname: Qin
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hua-Bin
authors.initials: HB
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018899:CpG Islands / Q000235:genetics*; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D019175:DNA Methylation; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D051548:Histone Acetyltransferases / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D056284:Histone Deacetylase 1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D051379:Mice; D009865:Oocytes / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D058989:Vitrification
chemical_list: D012333:RNA, Messenger; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619381:Dnmt1 protein, mouse; D051548:Histone Acetyltransferases; C110880:histone acetyltransferase type B complex; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1