title: Development of a DNA microarray for species identification of quarantine aphids.
abstract: BACKGROUND
  Aphid pests are being brought into Korea as a result of increased crop trading. Aphids exist on growth areas of plants, and thus plant growth is seriously affected by aphid pests. However, aphids are very small and have several sexual morphs and life stages, so it is difficult to identify species on the basis of morphological features. This problem was approached using DNA microarray technology.
  
  
  RESULTS
  DNA targets of the cytochrome c oxidase subunit I gene were generated with a fluorescent dye-labelled primer and were hybridised onto a DNA microarray consisting of specific probes. After analysing the signal intensity of the specific probes, the unique patterns from the DNA microarray, consisting of 47 species-specific probes, were obtained to identify 23 aphid species. To confirm the accuracy of the developed DNA microarray, ten individual blind samples were used in blind trials, and the identifications were completely consistent with the sequencing data of all individual blind samples.
  
  
  CONCLUSIONS
  A microarray has been developed to distinguish aphid species. DNA microarray technology provides a rapid, easy, cost-effective and accurate method for identifying aphid species for pest control management.
authors.affiliation: Department of Biochemistry, Hanyang University and GenoCheck Co., Ltd, Sangrok-gu, Ansan, Gyeonggi-do, Republic of Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Lee
authors.forename: Hwalran
authors.initials: H
authors.lastname: Choi
authors.forename: Jinseok
authors.initials: J
authors.lastname: Kang
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Si Hyeock
authors.initials: SH
authors.lastname: Lee
authors.forename: Seunghwan
authors.initials: S
authors.lastname: Lee
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D001042:Aphids / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011790:Quarantine / Q000662:veterinary; D056910:Republic of Korea
chemical_list: D017931:DNA Primers; D019476:Insect Proteins; D003576:Electron Transport Complex IV
keywords: COI; DNA microarray; aphids; quarantine; species identification