title: Inflammatory Regulation Effect and Action Mechanism of Anti-Inflammatory Effective Parts of Housefly (Musca domestica) Larvae on Atherosclerosis.
abstract: The protein-enriched extracts of housefly larvae were segregated by gel-filtration chromatography (GFC) and then anti-inflammatory activity screening in RAW264.7 (induced by LPS) was carried out. After acquire the anti-inflammatory effective parts, its anti-atherosclerotic properties in vivo were then evaluated. Results showed that the anti-inflammatory effective parts of housefly larvae were low-molecular-weight parts. After treated with the effective parts oral gavaged for 4 weeks, the atherosclerotic lesions of the mouse were significantly decreased. The inflammatory and lipid parameters were also reduced (except HDL which was increased). Western blot analysis demonstrated that the effective parts exerted potent inhibitory effect on expression of p65 in nucleus and cytoplasm. The results of immunofluorescence microscopy analysis also showed that the expressions of p65 both in cytoplasm and nucleus were significantly reduced. The hypothesis that the anti-inflammatory effective parts of housefly larvae possessed anti-atherosclerosis activity in mouse and the possible mechanism could be associated with the inhibition of expression and nuclear transfer of NF- κ B p65 could be derived.
authors.affiliation: Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 280 Wai Huan Dong Road, Guangzhou 510006, China ; School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 280 Wai Huan Dong Road, Guangzhou 510006, China.
authors.forename: Fu Jiang
authors.initials: FJ
authors.lastname: Chu
authors.forename: Xiao Bao
authors.initials: XB
authors.lastname: Jin
authors.forename: Yin Ye
authors.initials: YY
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Xiao Bo
authors.initials: XB
authors.lastname: Li
authors.forename: Xue Mei
authors.initials: XM
authors.lastname: Lu
authors.forename: Wen Bin
authors.initials: WB
authors.lastname: Liu
authors.forename: Jia Yong
authors.initials: JY
authors.lastname: Zhu
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