title: High level expression, purification and characterization of recombinant CCR5 as a vaccine candidate against HIV.
abstract: Cysteine-cysteine chemokine receptor type 5 (CCR5) is an important co-receptor for human immunodeficiency virus (HIV) infection and CCR5 neutralizing agents have proven efficient in patients suffering from HIV infection. Here, we expressed and purified various CCR5 vaccines named rCCR5, PADRE-rCCR5, GST-C1 and GST-C2 composed of different epitopes of CCR5. Results showed that vaccines containing multiple epitopes (rCCR5 and PADRE-rCCR5) induced stronger immune responses than single-epitope ones (GST-C1 and GST-C2). In addition, the elicited antibodies can specifically bind CCR5(+) U937 but not CCR5(-) Wish cells. These results demonstrate that the CCR5 vaccines are useful for further research, especially for the in vitro preclinical evaluation of their potential as biological CCR5 neutralizing agents.
authors.affiliation: State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Kongtian
authors.initials: K
authors.lastname: Wu
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Xue
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Qin
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Weina
authors.initials: W
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hao
authors.forename: Zenglu
authors.initials: Z
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D016915:AIDS Vaccines / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D041702:BALB 3T3 Cells; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D004926:Escherichia coli / Q000235:genetics; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D019713:Receptors, CCR5 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use
chemical_list: D016915:AIDS Vaccines; D000939:Epitopes; D019713:Receptors, CCR5; D011994:Recombinant Proteins