title: Detection of mouse cough based on sound monitoring and respiratory airflow waveforms.
abstract: Detection for cough in mice has never yielded clearly audible sounds, so there is still a great deal of debates as to whether mice can cough in response to tussive stimuli. Here we introduce an approach for detection of mouse cough based on sound monitoring and airflow signals. 40 Female BALB/c mice were pretreated with normal saline, codeine, capasazepine or desensitized with capsaicin. Single mouse was put in a plethysmograph, exposed to aerosolized 100 µmol/L capsaicin for 3 min, followed by continuous observation for 3 min. Airflow signals of total 6 min were recorded and analyzed to detect coughs. Simultaneously, mouse cough sounds were sensed by a mini-microphone, monitored manually by an operator. When manual and automatic detection coincided, the cough was positively identified. Sound and sound waveforms were also recorded and filtered for further analysis. Body movements were observed by operator. Manual versus automated counts were compared. Seven types of airflow signals were identified by integrating manual and automated monitoring. Observation of mouse movements and analysis of sound waveforms alone did not produce meaningful data. Mouse cough numbers decreased significantly after all above drugs treatment. The Bland-Altman and consistency analysis between automatic and manual counts was 0.968 and 0.956. The study suggests that the mouse is able to present with cough, which could be detected by sound monitoring and respiratory airflow waveform changes.
authors.affiliation: Department of Respiratory Diseases, The 1st Affiliated Hospital of Guangzhou Medical College, Guangzhou, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Chen
authors.forename: Kefang
authors.initials: K
authors.lastname: Lai
authors.forename: Joseph Mark
authors.initials: JM
authors.lastname: Lomask
authors.forename: Bert
authors.initials: B
authors.lastname: Jiang
authors.forename: Nanshan
authors.initials: N
authors.lastname: Zhong
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology; D003061:Codeine / Q000494:pharmacology; D003371:Cough / Q000175:diagnosis* / Q000503:physiopathology / Q000517:prevention & control; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010993:Plethysmography, Whole Body / Q000295:instrumentation* / Q000379:methods; D012123:Pulmonary Ventilation / Q000187:drug effects / Q000502:physiology*; D012135:Respiratory Sounds / Q000175:diagnosis*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D012965:Sodium Chloride; C071423:capsazepine; D002211:Capsaicin; D003061:Codeine
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