title: Amplification of antioxidant activity of haptoglobin(2-2)-hemoglobin at pathologic temperature and presence of antibiotics.
abstract: It is clear that Haptoglobin binds to Hemoglobin strongly and irreversibly. This binding, protects body tissues against heme-mediated oxidative tissue damages via peroxidase activity of Haptoglobin-Hemoglobin complex. Peroxidase activity of Haptoglobin(2-2)-Hemoglobin complex was determined via measurement of following increase in absorption of produced tetraguaiacol as the second substrate of Haptoglobin-Hemoglobin complex by UV-Vis spectrophotometer at 470 nm and 42°C. The results are showing that peroxidase activity of Haptoglobin(2-2)-Hemoglobin complex is modulated by homotropic effect of hydrogen peroxide as the allosteric substrate. On the other hand, antioxidant activity of Haptoglobin(2-2)-Hemoglobin is increased via heterotropic effect of two antibiotics (especially ampicillin) on the peroxidase activity of the complex. The condition of pathologic temperature along with the administration of ampicillin and/or coamoxiclav is in favor of amplification in antioxidant activity of Haptoglobin(2-2)-Hemoglobin and combating against free radicals in individuals with Hp2-2 phenotype. Therefore, oxidative stress effects have been diminished in the population with this phenotype.
authors.affiliation: Department of Biology, Faculty of Basic Science, Alzahra University, Tehran, Iran ; Departments of Genetics and Pathology, University Medical Centre Groningen, University of Groningen, Groningen, Netherlands.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Tayari
authors.forename: Danial
authors.initials: D
authors.lastname: Afsharzadeh
keywords: Antibiotics; Antioxidant; Haptoglobin; Hemoglobin; Pathologic
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