title: Composition and expression of genes encoding carbohydrate-active enzymes in the straw-degrading mushroom Volvariella volvacea.
abstract: Volvariella volvacea is one of a few commercial cultivated mushrooms mainly using straw as carbon source. In this study, the genome of V. volcacea was sequenced and assembled. A total of 285 genes encoding carbohydrate-active enzymes (CAZymes) in V. volvacea were identified and annotated. Among 15 fungi with sequenced genomes, V. volvacea ranks seventh in the number of genes encoding CAZymes. In addition, the composition of glycoside hydrolases in V. volcacea is dramatically different from other basidiomycetes: it is particularly rich in members of the glycoside hydrolase families GH10 (hemicellulose degradation) and GH43 (hemicellulose and pectin degradation), and the lyase families PL1, PL3 and PL4 (pectin degradation) but lacks families GH5b, GH11, GH26, GH62, GH93, GH115, GH105, GH9, GH53, GH32, GH74 and CE12. Analysis of genome-wide gene expression profiles of 3 strains using 3'-tag digital gene expression (DGE) reveals that 239 CAZyme genes were expressed even in potato destrose broth medium. Our data also showed that the formation of a heterokaryotic strain could dramatically increase the expression of a number of genes which were poorly expressed in its parental homokaryotic strains.
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Bingzhi
authors.initials: B
authors.lastname: Chen
authors.forename: Fu
authors.initials: F
authors.lastname: Gui
authors.forename: Baogui
authors.initials: B
authors.lastname: Xie
authors.forename: Youjin
authors.initials: Y
authors.lastname: Deng
authors.forename: Xianyun
authors.initials: X
authors.lastname: Sun
authors.forename: Mengying
authors.initials: M
authors.lastname: Lin
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Tao
authors.forename: Shaojie
authors.initials: S
authors.lastname: Li
mesh_terms: D050260:Carbohydrate Metabolism / Q000235:genetics*; D016000:Cluster Analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D016681:Genome, Fungal; D055367:Volvariella / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
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