title: Characterization and endocrine regulation of proliferation and differentiation of primary cultured preadipocytes from gilthead sea bream (Sparus aurata).
abstract: A preadipocyte primary cell culture was established to gain knowledge about adipose tissue development in gilthead sea bream (Sparus aurata), one of the most extensively produced marine aquaculture species in the Mediterranean. The preadipocytes obtained from the stromal-vascular cell fraction of adipose tissue proliferated in culture, reaching confluence around day 8. At that time, the addition of an adipogenic medium promoted differentiation of the cells into mature adipocytes, which showed an enlarged cytoplasm filled with lipid droplets. First, cell proliferation and differentiation were analyzed under control and adipogenic conditions during culture development. Next, the effects of insulin, GH, and IGF-I on cell proliferation were evaluated at day 8. All peptides significantly stimulated proliferation of the cells after 48 h of incubation (P < 0.002 for GH and IGF-I and P < 0.05 for insulin), despite no differences were observed between the different doses tested. Subsequently, the effects of insulin and IGF-I maintaining differentiation when added to growth medium were studied at day 11, after 3 d of induction with adipogenic medium. The results showed that IGF-I is more potent than insulin enhancing differentiation (P < 0.01 for IGF-I compared with the control). In summary, a primary culture of gilthead sea bream preadipocytes has been characterized and the effects of several regulators of growth and development have been evaluated. This cellular system can be a good model to study the process of adipogenesis in fish, which may help improve the quality of the product in aquaculture.
authors.affiliation: Department of Physiology and Immunology, Faculty of Biology, University of Barcelona, Av. Diagonal 643, Barcelona 08028, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Salmerón
authors.forename: L
authors.initials: L
authors.lastname: Acerete
authors.forename: J
authors.initials: J
authors.lastname: Gutiérrez
authors.forename: I
authors.initials: I
authors.lastname: Navarro
authors.forename: E
authors.initials: E
authors.lastname: Capilla
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D017756:Aquaculture; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D013006:Growth Hormone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D008954:Models, Biological; D021541:Sea Bream / Q000502:physiology*
chemical_list: D007328:Insulin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone