title: High performance thin layer chromatography-densitometry: a step further for quality control of cranberry extracts.
abstract: A method for selective quantitation of catechin, proanthocyanidin (PAC) A2 and PAC-B1 in American cranberry (Vaccinium macrocarpon) extracts using high performance thin layer chromatography (HPTLC)-densitometry is presented. Methylene chloride/ethyl acetate/formic acid (6:10:1, v/v) as the mobile phase and 1% vanillin hydrochloric solution as staining reagent were used. In these conditions, three standards considered as quality markers, catechin, PAC-A2 and PAC-B1, were well resolved allowing simultaneous quantitation on one plate. All standards were quantified in the range of 0.7-5 μg with RSD of repeatability and intermediate precision not exceeding 5%. Catechin, PAC-A2 and PAC-B1 profiles of cranberry extracts were analysed regarding global PAC amounts obtained by BL-DMAC assay. It appears clearly that HPTLC-densitometry process provides additional information which, combined with BL-DMAC results, allows qualitative and quantitative control of cranberry extracts. Particularly, densitometric assay highlighted degradation of PACs in a 7 day-extract, leading to high overestimation with BL-DMAC protocol.
authors.affiliation: UMR INRA 1282 Infectiologie et Santé Publique, Equipe Recherche et Innovation en Chimie Médicinale, UFR Sciences Pharmaceutiques, Université de Tours François Rabelais, 31 Avenue Monge, 37200 Tours, France.
authors.forename: Leslie
authors.initials: L
authors.lastname: Boudesocque
authors.forename: Joëlle
authors.initials: J
authors.lastname: Dorat
authors.forename: Jacques
authors.initials: J
authors.lastname: Pothier
authors.forename: Alain
authors.initials: A
authors.lastname: Gueiffier
authors.forename: Cécile
authors.initials: C
authors.lastname: Enguehard-Gueiffier
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D010936:Plant Extracts