title: Highly sensitive fluorescent protein FRET detection using optofluidic lasers.
abstract: We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.
authors.affiliation: Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA.
authors.forename: Qiushu
authors.initials: Q
authors.lastname: Chen
authors.forename: Xingwang
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuze
authors.initials: Y
authors.lastname: Sun
authors.forename: Michael
authors.initials: M
authors.lastname: Ritt
authors.forename: Sivaraj
authors.initials: S
authors.lastname: Sivaramakrishnan
authors.forename: Xudong
authors.initials: X
authors.lastname: Fan
mesh_terms: D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007834:Lasers; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein