title: Pectin-chitosan-PVA nanofibrous scaffold made by electrospinning and its potential use as a skin tissue scaffold.
abstract: Scaffolds made of chitosan nanofibers are often too mechanically weak for their application and often their manufacturing processes involve the use of harmful and flammable organic solvents. In the attempt to improve the mechanical properties of nanofibrous scaffolds made of chitosan without the use of harmful chemicals, pectin, an anionic polymer was blended with chitosan, a cationic polymer, to form a polyelectrolyte complex and electrospun into nanofibers for the first time. The electrospun chitosan-pectin scaffolds, when compared to electrospun chitosan scaffolds, had a 58% larger diameter, a 21% higher Young's modulus, a 162% larger strain at break, and a 104% higher ultimate tensile strength. Compared to the chitosan scaffolds, the chitosan-pectin scaffolds' swelling ratios decreased by 55% after 60 min in a saline solution and more quickly released the preloaded tetracycline HCl. The L929 fibroblast cells proliferated slightly slower on the chitosan-pectin scaffolds than on the chitosan scaffolds. Nonetheless, cells on both materials deposited similar levels of extracellular type I collagen on a per DNA basis. In conclusion, a novel chitosan-pectin nanofibrous scaffold with superior mechanical properties than a chitosan nanofibrous scaffold was successfully made without the use of harmful solvents.
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Sec 3, Zhongxiao E. Rd, Taipei 106, Taiwan. hbrunken@ntut.edu.tw
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Lin
authors.forename: Hsin-Hung
authors.initials: HH
authors.lastname: Chen
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Chang
authors.forename: Tsung-Sheng
authors.initials: TS
authors.lastname: Ni
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D024042:Collagen Type I / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry; D055664:Electrochemical Techniques; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010368:Pectins / Q000737:chemistry*; D017768:Skin, Artificial; D013718:Tensile Strength; D013752:Tetracycline / Q000008:administration & dosage; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D024042:Collagen Type I; D004337:Drug Carriers; D010368:Pectins; D048271:Chitosan; D013752:Tetracycline