title: Making ends meet: chemically mediated circularization of recombinant proteins.
abstract: A selective N→S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.
authors.affiliation: Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London, WC1H 0AJ, UK.
authors.forename: Ben
authors.initials: B
authors.lastname: Cowper
authors.forename: David J
authors.initials: DJ
authors.lastname: Craik
authors.forename: Derek
authors.initials: D
authors.lastname: Macmillan
mesh_terms: D003500:Cyclization; D047169:Cyclotides / Q000096:biosynthesis* / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D047169:Cyclotides; D011994:Recombinant Proteins; C091930:kalata B1
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