title: Genetic regulation of glucoraphanin accumulation in Beneforté broccoli.
abstract: · Diets rich in broccoli (Brassica oleracea var italica) have been associated with maintenance of cardiovascular health and reduction in risk of cancer. These health benefits have been attributed to glucoraphanin that specifically accumulates in broccoli. The development of broccoli with enhanced concentrations of glucoraphanin may deliver greater health benefits. · Three high-glucoraphanin F1 broccoli hybrids were developed in independent programmes through genome introgression from the wild species Brassica villosa. Glucoraphanin and other metabolites were quantified in experimental field trials. Global SNP analyses quantified the differential extent of B. villosa introgression · The high-glucoraphanin broccoli hybrids contained 2.5-3 times the glucoraphanin content of standard hybrids due to enhanced sulphate assimilation and modifications in sulphur partitioning between sulphur-containing metabolites. All of the high-glucoraphanin hybrids possessed an introgressed B. villosa segment which contained a B. villosa Myb28 allele. Myb28 expression was increased in all of the high-glucoraphanin hybrids. Two high-glucoraphanin hybrids have been commercialised as Beneforté broccoli. · The study illustrates the translation of research on glucosinolate genetics from Arabidopsis to broccoli, the use of wild Brassica species to develop cultivars with potential consumer benefits, and the development of cultivars with contrasting concentrations of glucoraphanin for use in blinded human intervention studies.
authors.affiliation: Food & Health Programme, Institute of Food Research, Norwich Research Park, NR4 7UA, UK.
authors.forename: Maria H
authors.initials: MH
authors.lastname: Traka
authors.affiliation: Food & Health Programme, Institute of Food Research, Norwich Research Park, NR4 7UA, UK.
authors.forename: Shikha
authors.initials: S
authors.lastname: Saha
authors.affiliation: Food & Health Programme, Institute of Food Research, Norwich Research Park, NR4 7UA, UK.|Metabolic Biology, John Innes Centre, Norwich Research Park, NR4 7UH, UK.
authors.forename: Stine
authors.initials: S
authors.lastname: Huseby
authors.affiliation: Metabolic Biology, John Innes Centre, Norwich Research Park, NR4 7UH, UK.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kopriva
authors.affiliation: Warwick Life Sciences, The University of Warwick, Wellesbourne, Warwick, CV35 9EF, UK.
authors.forename: Peter G
authors.initials: PG
authors.lastname: Walley
authors.affiliation: Warwick Life Sciences, The University of Warwick, Wellesbourne, Warwick, CV35 9EF, UK.
authors.forename: Guy C
authors.initials: GC
authors.lastname: Barker
authors.affiliation: Warwick Systems Biology, The University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Moore
authors.affiliation: Seminis Vegetable Seeds, Inc., Arroyo Grande, CA, 93420, USA.
authors.forename: Gene
authors.initials: G
authors.lastname: Mero
authors.affiliation: Seminis Vegetable Seeds, Inc., Wageningse Afweg 31, 6702 PD, Wageningen, the Netherlands.
authors.forename: Frans
authors.initials: F
authors.lastname: van den Bosch
authors.affiliation: Monsanto Center for Food and Nutrition Research, Seminis Vegetable Seeds, Inc., Kannapolis, NC, 28081, USA.
authors.forename: Howard
authors.initials: H
authors.lastname: Constant
authors.affiliation: Seminis Vegetable Seeds, Inc., Woodland, CA, 95695, USA.
authors.forename: Leo
authors.initials: L
authors.lastname: Kelly
authors.affiliation: Seminis Vegetable Seeds, Inc., Wageningse Afweg 31, 6702 PD, Wageningen, the Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Schepers
authors.affiliation: Seminis Vegetable Seeds, Inc., Woodland, CA, 95695, USA.
authors.forename: Sekhar
authors.initials: S
authors.lastname: Boddupalli
authors.affiliation: Food & Health Programme, Institute of Food Research, Norwich Research Park, NR4 7UA, UK.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Mithen
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000235:genetics* / Q000378:metabolism*; D001947:Breeding; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D035264:Flowers / Q000378:metabolism; D005502:Food; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006824:Hybridization, Genetic; D007096:Imidoesters / Q000737:chemistry / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D010091:Oximes; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D013454:Sulfoxides; D013455:Sulfur / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D005961:Glucosinolates; D007096:Imidoesters; D010091:Oximes; D010940:Plant Proteins; D013454:Sulfoxides; D014157:Transcription Factors; D013455:Sulfur; D008715:Methionine; C119494:glucoraphanin
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