title: Rapid detection of exon 2-deleted AIMP2 mutation as a potential biomarker for lung cancer by molecular beacons.
abstract: Exon 2 deletion in aminoacyl tRNA synthetase complex-interacting multifunctional protein 2 (AIMP2) has been suggested to be associated with the progression of various cancers such as lung and ovarian cancers. However, few studies have been conducted regarding detection and relevance of exon 2-deleted AIMP2 (AIMP2-DX2) mutation to a specific cancer. Here, we demonstrate the rapid and simple detection of the AIMP2-DX2 mutation by molecular beacons and its relation to lung cancer. Real-time PCR with molecular beacons allowed a sensitive detection of the AIMP2-DX2 mutation as low as 0.3 pg initial template. Dual-conjugated liposomes with folate and molecular beacon enabled fluorescence imaging of cancer cells harboring the AIMP2-DX2 mutation with high resolution. Association of the AIMP2-DX2 mutation with lung cancer was shown by analyzing tissue samples from lung cancer patients using real-time PCR. Approximately, 60% of lung cancer patients harbored the AIMP2-DX2 mutation, which implies a potential of the AIMP2-DX2 mutation as a prognostic biomarker for lung cancer. Molecular beacon-based approaches will find applications in the simple and rapid detection of mutations on nucleotides for diagnosing and monitoring the progression of relevant cancer.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Jo
authors.forename: Youngwook
authors.initials: Y
authors.lastname: Kim
authors.forename: Young-Su
authors.initials: YS
authors.lastname: Jeong
authors.forename: Young
authors.initials: Y
authors.lastname: Hee Oh
authors.forename: Keunchil
authors.initials: K
authors.lastname: Park
authors.forename: Hak-Sung
authors.initials: HS
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D005091:Exons; D005492:Folic Acid / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics*; D009687:Nuclear Proteins; D061848:Optical Imaging / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017384:Sequence Deletion
chemical_list: C575685:AIMP2 protein, human; D002352:Carrier Proteins; D008081:Liposomes; D009687:Nuclear Proteins; D005492:Folic Acid