title: Defining the transcriptional and cellular landscape of type 1 diabetes in the NOD mouse.
abstract: Our ability to successfully intervene in disease processes is dependent on definitive diagnosis. In the case of autoimmune disease, this is particularly challenging because progression of disease is lengthy and multifactorial. Here we show the first chronological compendium of transcriptional and cellular signatures of diabetes in the non-obese diabetic mouse. Our data relates the immunological environment of the islets of Langerhans with the transcriptional profile at discrete times. Based on these data, we have parsed diabetes into several discrete phases. First, there is a type I interferon signature that precedes T cell activation. Second, there is synchronous infiltration of all immunological cellular subsets and a period of control. Finally, there is the killing phase of the diabetogenic process that is correlated with an NF-kB signature. Our data provides a framework for future examination of autoimmune diabetes and its disease progression markers.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, United States of America. jacarrer@wustl.edu
authors.forename: Javier A
authors.initials: JA
authors.lastname: Carrero
authors.forename: Boris
authors.initials: B
authors.lastname: Calderon
authors.forename: Fadi
authors.initials: F
authors.lastname: Towfic
authors.forename: Maxim N
authors.initials: MN
authors.lastname: Artyomov
authors.forename: Emil R
authors.initials: ER
authors.lastname: Unanue
mesh_terms: D000818:Animals; D002453:Cell Cycle; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism; D007370:Interferon Type I / Q000378:metabolism; D007515:Islets of Langerhans / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D016688:Mice, Inbred NOD; D022423:Myeloid Cells / Q000276:immunology; D060066:Protein Interaction Maps; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D018398:Homeodomain Proteins; D007370:Interferon Type I; C064658:RAG-1 protein
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