title: UVA1 irradiation inhibits fibroblast proliferation and alleviates pathological changes of scleroderma in a mouse model.
abstract: The purpose of the present study was to compare the effects of different doses of ultraviolet radiation A1 (UVA1) on human fibroblast proliferation and collagen level in a mouse model of scleroderma, so as to identify appropriate irradiation doses for clinical treatment of scleroderma. Monolayer from human fibroblasts was cultured in vitro, and a mouse model of scleroderma was established by subcutaneous injection of 100 µL of 400 µg/mL bleomycin into the back of BALB/c mice for 4 weeks. The mouse models and human fibroblasts were divided into UVA1-exposed (100, 60 and 20 J/cm(2)) and UVA-unexposed groups. At 0, 24 and 48 h after exposure, cell proliferation and levels of hydroxyproline and collagen were detected. UVA1 irradiation was performed 3 times weekly for 10 weeks, and the pathological changes of skin tissues, skin thickness and collagen level were observed after phototherapy. Cell proliferation and the levels of hydroxyproline and collagen were inhibited after phototherapy, and there was a significant difference between the UVA1-exposed cells and UVA1-unexposed cells (P < 0.001). In addition, UVA1 phototherapy improved dermal sclerosis and softened the skin, and there were significant differences between the high-dose UVA1 group and the model group, and the negative group (P < 0.05). It is concluded that UVA1 radiation can reduce cell proliferation, and decrease hydroxyproline and collagen levels in a dose-dependent manner in vitro. High-dose UVA1 phototherapy has marked therapeutic effect on scleroderma in the mouse model. Decreased collagen level may be related to the reduced number and activity of cells, as well as inhibition of collagen synthesis.
authors.affiliation: Institute of Dermatology, Chinese Academy of Medical Science, Nanjing Jiangsu 210042, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ju
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
authors.forename: Baozhu
authors.initials: B
authors.lastname: Chang
authors.forename: Heng
authors.initials: H
authors.lastname: Gu
keywords: fibroblasts; mouse model; proliferation; scleroderma; ultraviolet irradiation A1 (UVA1)
references.citation: J Invest Dermatol. 2001 Dec;117(6):1458-63
references.pmid: 11886509
references.citation: Br J Dermatol. 2007 May;156(5):951-6
references.pmid: 17381457
references.citation: Photodermatol Photoimmunol Photomed. 2009 Jun;25(3):153-5
references.pmid: 19438995
references.citation: J Am Acad Dermatol. 2000 Oct;43(4):670-4
references.pmid: 11004624
references.citation: Br J Dermatol. 2010 Feb 1;162(2):445-7
references.pmid: 19785603
references.citation: Eur J Cell Biol. 1992 Apr;57(2):244-53
references.pmid: 1511700
references.citation: Photodermatol Photoimmunol Photomed. 2004 Apr;20(2):93-100
references.pmid: 15030594
references.citation: Methods Enzymol. 2000;319:302-9
references.pmid: 10907522
references.citation: Lancet. 1997 Nov 1;350(9087):1295-6
references.pmid: 9357412
references.citation: Curr Opin Rheumatol. 2011 Nov;23(6):511-8
references.pmid: 21857225
references.citation: Photodermatol Photoimmunol Photomed. 2007 Dec;23(6):215-21
references.pmid: 17986056
references.citation: J Am Acad Dermatol. 1998 Jan;38(1):21-6
references.pmid: 9448200
references.citation: Inflamm Res. 2001 Aug;50(8):422-7
references.pmid: 11556523
references.citation: Br J Dermatol. 2006 Sep;155(3):600-7
references.pmid: 16911288
references.citation: Int J Dermatol. 2011 Aug;50(8):1006-13
references.pmid: 21781079
references.citation: J Am Acad Dermatol. 2009 May;60(5):786-91
references.pmid: 19211170
references.citation: J Dermatol. 2003 Mar;30(3):173-80
references.pmid: 12692352
references.citation: Dermatology. 2011;222(4):347-57
references.pmid: 21757879
references.citation: J Invest Dermatol. 1999 Apr;112(4):456-62
references.pmid: 10201529
references.citation: Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5953-8
references.pmid: 17389394
references.citation: Ann Dermatol. 2010 Feb;22(1):1-8
references.pmid: 20548873
references.citation: J Am Acad Dermatol. 1997 Jun;36(6 Pt 1):938-44
references.pmid: 9204059
references.citation: J Dermatol Sci. 2005 Mar;37(3):137-43
references.pmid: 15734282