title: Lack of galectin-1 or galectin-3 alters B cell deletion and anergy in an autoantibody transgene model.
abstract: Members of the galectin family of proteins have been shown to regulate the development and the function of immune cells. We previously identified the increased expression of galectin-1 and galectin-3 mRNA and protein in anergic B cells relative to their naïve counterparts. To investigate the role of these galectins in maintaining B cell tolerance, we crossed mice deficient in galectin-1 or galectin-3 with mice bearing a lupus autoantigen-binding transgenic (Tg) B cell receptor, using a model with a well-characterized B cell tolerance phenotype of deletion, receptor editing and anergy. Here, we present data showing that the global knockout of galectin-1 or galectin-3 yields subtle alterations in B cell fate in autoantibody Tg mice. The absence of galectin-3 leads to a significant increase in the number of Tg spleen B cells, with the recovery of anti-laminin antibodies from a subset of mice. The B cell number increases further in antibody Tg mice with the dual deficiency of both galectin-1 and galectin-3. Isolated galectin-1 deficiency significantly enhances the proliferation of Tg B cells in response to lipopolysaccharide stimulation. These findings add to the growing body of evidence indicating a role for the various galectin family members, and for galectins 1 and 3 in particular, in the regulation of autoimmunity.
authors.affiliation: Department of Medicine, Duke University Medical Center, and Research Service, Durham VA Medical Center, Box 103015, Durham, NC 27710, USA.
authors.forename: Amy G
authors.initials: AG
authors.lastname: Clark
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Weston
authors.forename: Mary H
authors.initials: MH
authors.lastname: Foster
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D017635:Clonal Anergy / Q000235:genetics*; D017637:Clonal Deletion / Q000235:genetics*; D037483:Galectin 1 / Q000235:genetics*; D037502:Galectin 3 / Q000235:genetics*; D007797:Laminin / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D019076:Transgenes
chemical_list: D001323:Autoantibodies; D037483:Galectin 1; D037502:Galectin 3; D007797:Laminin; C485095:Lgals3 protein, mouse
keywords: CD62L; CD86; MHCII; autoimmunity; humoral immunity
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