title: Substrate recognition in selective autophagy and the ubiquitin-proteasome system.
abstract: Dynamic protein turnover through regulated protein synthesis and degradation ensures cellular growth, proliferation, differentiation and adaptation. Eukaryotic cells utilize two mechanistically distinct but largely complementary systems - the 26S proteasome and the lysosome (or vacuole in yeast and plants) - to effectively target a wide range of proteins for degradation. The concerted action of the ubiquitination machinery and the 26S proteasome ensures the targeted and tightly regulated degradation of a subset of commonly short-lived cellular proteins. Autophagy is a distinct degradation pathway, which transports a highly heterogeneous set of cargos in dedicated vesicles, called autophagosomes, to the lysosome. There the cargo becomes degraded and its molecular building blocks are recycled. While general autophagy randomly engulfs portions of the cytosol, selective autophagy employs dedicated cargo adaptors to specifically enrich the forming autophagosomes for a certain type of cargo as a response to various intra- or extracellular signals. Selective autophagy targets a wide range of cargos including long-lived proteins and protein complexes, organelles, protein aggregates and even intracellular microbes. In this review we summarize available data on cargo recognition mechanisms operating in selective autophagy and the ubiquitin-proteasome system (UPS), and emphasize their differences and common themes. Moreover, we derive general regulatory principles underlying cargo recognition in selective autophagy, and describe the system-wide crosstalk between these two cellular protein degradation systems. This article is part of a Special Issue entitled: Ubiquitin-Proteasome System. Guest Editors: Thomas Sommer and Dieter H. Wolf.
authors.affiliation: Institute of Biochemistry, HPM, ETH Hönggerberg, 8093 Zürich, Switzerland.
authors.forename: Anne
authors.initials: A
authors.lastname: Schreiber
authors.forename: Matthias
authors.initials: M
authors.lastname: Peter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D025801:Ubiquitin / Q000378:metabolism; D054875:Ubiquitination / Q000502:physiology
chemical_list: D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex
keywords: Posttranslational modification; Protein degradation; Selective autophagy; Substrate recognition; Ubiquitination; Ubiquitin–proteasome system