title: Protective effect of the polysaccharide from Ophiopogon japonicus on streptozotocin-induced diabetic rats.
abstract: The present study was designed to investigate the effect of OJP1, a polysaccharide isolated from the roots of Ophiopogon japonica, on blood lipid metabolism, antioxidant activity, as well as its protective effect on the liver and kidneys in diabetic rats. Results showed that OJP1 significantly reduced the MDA concentration and increased the activity of both GPx and SOD in the serum, liver and kidneys of diabetic rats. Moreover, the values of TG, TC, LDL-C and HDL-C in diabetic rats were significantly reversed by OJP1 treatment. Biochemical and histopathological analyses also showed that OJP1 can alleviate liver and kidneys injury in diabetic rats. The mRNA expression of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) in diabetic rats decreased significantly after administration of OJP1. Altogether, these results suggest that OJP1 possess potent antioxidant activity and can protect the liver and kidneys from the injurious effects of diabetes.
authors.affiliation: Institute of Glycobiological Engineering/School of Laboratory Medicine & Life Science, Wenzhou Medical College, Wenzhou 325035, China. xmchen01@163.com
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Tang
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Xie
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Liqin
authors.initials: L
authors.lastname: Jin
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000097:blood; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007668:Kidney / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D031441:Ophiopogon / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000097:blood; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D001786:Blood Glucose; C526316:CCN2 protein, rat; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D008055:Lipids; D011134:Polysaccharides; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D008315:Malondialdehyde; D013311:Streptozocin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase