title: Implications in the difference of anti-Mi-2 and -p155/140 autoantibody prevalence in two dermatomyositis cohorts from Mexico City and Guadalajara.
abstract: BACKGROUND
  Autoantibodies and clinical manifestations in polymyositis/dermatomyositis (PM/DM) are affected by both genetic and environmental factors. The high prevalence of DM and anti-Mi-2 in Central America is thought to be associated with the high UV index of the area. The prevalences of autoantibodies and the clinical manifestations of PM/DM were evaluated comparing two cohorts in Mexico.
  
  
  METHODS
  Ninety-five Mexican patients with PM/DM (66 DM, 29 PM; 67 Mexico City, 28 Guadalajara) were studied. Autoantibodies were characterized by immunoprecipitation using 35S-methionine labeled K562 cell extract. Clinical information was obtained from medical records.
  
  
  RESULTS
  DM represented 69% of PM/DM and anti-Mi-2 was the most common autoantibody (35%), followed by anti-p155/140 (11%); however, anti-Jo-1 was only 4%. The autoantibody profile in adult-onset DM in Mexico City versus Guadalajara showed striking differences: anti-Mi-2 was 59% versus 12% (P = 0.0012) whereas anti-p155/140 was 9% versus 35% (P = 0.02), respectively. A strong association of anti-Mi-2 with DM was confirmed and when clinical features of anti-Mi-2 (+) DM (n = 30) versus anti-Mi-2 (-) DM (n = 36) were compared, the shawl sign (86% versus 64%, P < 0.05) was more common in the anti-Mi-2 (+) group (P = 0.0001). Levels of creatine phosphokinase (CPK) were higher in those who were anti-Mi-2 (+) but they responded well to therapy.
  
  
  CONCLUSIONS
  Anti-Mi-2 has a high prevalence in Mexican DM and is associated with the shawl sign and high CPK. The prevalence of anti-Mi-2 and anti-p155/140 was significantly different in Mexico City versus Guadalajara, which have a similar UV index. This suggests roles of factors other than UV in anti-Mi-2 antibody production.
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: Petri
authors.forename: Minoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Beatriz T
authors.initials: BT
authors.lastname: Martin-Marquez
authors.forename: Raul
authors.initials: R
authors.lastname: Vargas-Ramírez
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Jara
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Saavedra
authors.forename: Claudia
authors.initials: C
authors.lastname: Cruz-Gonzalez
authors.forename: Lilia
authors.initials: L
authors.lastname: Andrade-Ortega
authors.forename: Olga
authors.initials: O
authors.lastname: Vera-Lastra
authors.forename: Mario
authors.initials: M
authors.lastname: Salazar-Páramo
authors.forename: Rosa E
authors.initials: RE
authors.lastname: Prieto-Parra
authors.forename: Laura
authors.initials: L
authors.lastname: Gonzalez-Lopez
authors.forename: Jorge I
authors.initials: JI
authors.lastname: Gamez-Nava
authors.forename: Hermes U
authors.initials: HU
authors.lastname: Ramírez-Sánchez
authors.forename: Jason Y F
authors.initials: JY
authors.lastname: Chan
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Ross
authors.forename: Edward K L
authors.initials: EK
authors.lastname: Chan
authors.forename: Mónica
authors.initials: M
authors.lastname: Vázquez-Del Mercado
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D003882:Dermatomyositis / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D008800:Mexico / Q000453:epidemiology; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex / Q000276:immunology; D008875:Middle Aged; D015995:Prevalence
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; C115323:CHD4 protein, human; C045760:Mi-2 antibodies; C542777:p140 autoantigen, human; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex
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