title: Surface CD3 expression proceeds through both myosin regulatory light chain 9 (MYL9)-dependent and MYL9-independent pathways in Jurkat cells.
abstract: CD3 is a complex of polypeptides which form part of the T cell receptor. Normal human peripheral pan T cells, express not only CD3, but the mRNA for myosin regulatory light chains MYL9, MYL12A, and MYL12B are also significantly expressed. In the Jurkat wild strain, an acute T cell leukemia cell line, CD3 on the surface and MYL9 mRNA are not expressed, while both MYL12A mRNA and MYL12B mRNA are expressed. Jurkat-I, a new clone was established by the transfection of the MYL9 gene into the Jurkat wild strain. As a result, the level of CD3 expressed on the surface of Jurkat-I cells was significantly higher than those in the Jurkat wild strain. Phorbol 12-myristate 13-acetate increased the surface CD3 levels in Jurkat wild strain cells without resulting in MYL9 gene expression, indicating that protein kinase C is partially involved in the expression of CD3 on the surface. These results suggest that surface CD3 expression proceeds through both MYL9-dependent and MYL9-independent pathways (i.e. the protein kinase C- dependent pathway) in Jurkat cells.
authors.affiliation: Hematology, Clinical Medicine, Kitasato University Graduate School of Medical Science, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Aoki
authors.forename: Koji
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Takuji
authors.initials: T
authors.lastname: Katayama
authors.forename: Mariko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Horie
authors.forename: Mikio
authors.initials: M
authors.lastname: Danbara
authors.forename: Masaaki
authors.initials: M
authors.lastname: Higashihara
mesh_terms: D017252:CD3 Complex / Q000096:biosynthesis* / Q000235:genetics; D002273:Carcinogens / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D018994:Myosin Light Chains / Q000096:biosynthesis* / Q000235:genetics; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D017252:CD3 Complex; D002273:Carcinogens; D018994:Myosin Light Chains; D012333:RNA, Messenger; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate