title: Effect of L-arginine on HSP70 expression in liver in weanling piglets.
abstract: BACKGROUND
  This study was conducted to evaluate the effects of L-arginine (Arg) on photomicrographs and HSP70 expression in the liver of weanling piglets. Twelve healthy Landrace × Yorkshire piglets that had been weaned at 21 d (average body weight 5.56 ± 0.51 kg) were randomly divided into a control group and an Arg group (6 g/kg feed). At age 28 d, all of the piglets were slaughtered to obtain liver samples to determine HSP70 expression by real-time PCR, western blot and immunohistochemistry.
  
  
  RESULTS
  The results showed that, compared to control piglets, treatment with Arg decreased inflammatory reactions caused by weaning. The immunohistochemical localization of HSP70 in liver revealed strong expression in the Arg group. Arg increased HSP70 mRNA and HSP70 expression in the liver (P < 0.05).
  
  
  CONCLUSIONS
  These findings suggest that dietary supplementation with Arg could maintain liver health by inducing HSP70 expression in weanling piglets.
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xie
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.forename: Ruilin
authors.initials: R
authors.lastname: Huang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000818:Animals; D000833:Animals, Suckling / Q000276:immunology / Q000378:metabolism; D001120:Arginine / Q000494:pharmacology*; D015153:Blotting, Western / Q000662:veterinary; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000276:immunology / Q000378:metabolism; D014886:Weaning
chemical_list: D018840:HSP70 Heat-Shock Proteins; D001120:Arginine
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