title: Methods for isolation and identification of nanoparticle-containing subcellular compartments.
abstract: Nanoparticle-based drug delivery systems have considerable potential for improvement of drug stability, bioavailability, and reduced dosing frequency. Important technological advantages of nanoparticles include high carrier capacity across biological membranes and controlled drug release. Ultimately, success of nanodelivery systems depends on toxicologic issues associated with the understanding of the fate of nanocarriers and their polymeric constituents within the targeted cells. Here we describe a method for determining subcellular distribution of nanoparticles by isolation and identification of organelles that come in direct contact with these structures.
authors.forename: Ari
authors.initials: A
authors.lastname: Nowacek
authors.forename: Irena
authors.initials: I
authors.lastname: Kadiu
authors.forename: JoEllyn
authors.initials: J
authors.lastname: McMillan
authors.forename: Howard E
authors.initials: HE
authors.lastname: Gendelman
mesh_terms: D002451:Cell Compartmentation; D006801:Humans; D053758:Nanoparticles; D013347:Subcellular Fractions / Q000378:metabolism*
references.citation: Nanomedicine (Lond). 2009 Jul;4(5):541-55
references.pmid: 19572820
references.citation: Nanomedicine (Lond). 2009 Jul;4(5):557-74
references.pmid: 19572821
references.citation: Nanomedicine (Lond). 2011 Aug;6(6):975-94
references.pmid: 21417829