title: Potential anticancer activity of carvone in N2a neuroblastoma cell line.
abstract: Carvone (CVN) is a monocyclic monoterpene found in the essential oils of Mentha spicata var. crispa (Lamiaceae) and Carum carvi L. (Apiaceae) plants and has been reported to have antioxidant, antimicrobial, anticonvulsant, and antitumor activities. The beneficial health properties of CVN have encouraged us to look into its anticancer activity. To the best of our knowledge, reports are not available on the anticancer activity of CVN in cultured primary rat neuron and N2a neuroblastoma (NB) cells. Therefore, the present study is an attempt toward exploring the potential anticancer activity of CVN, if any, in cultured primary rat neuron and N2a NB cells. Our results indicated that CVN (only at 25 mg/L) treatment led to an increase in the total antioxidant capacity levels in cultured primary rat neuron cells compared with control cells. Also, CVN (at concentrations higher than 100 mg/L) treatment led to an increase in the total oxidative stress levels in both cell types. The mean values of the total scores of cells showing DNA damage (for comet assay) were not found to be significantly different from the control values in both cells (p > 0.05). On the other hand, after 24 h treatment with CVN, 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay showed that CVN application significantly reduced the cell viability rates in both cell types at concentrations higher than 100 mg/L. Summarizing, our data suggest that CVN represents little potential for promising anticancer agent to improve brain tumors therapy.
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey elanuraydinn@gmail.com.
authors.forename: Elanur
authors.initials: E
authors.lastname: Aydın
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Türkez
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey.
authors.forename: Mevlüt Sait
authors.initials: MS
authors.lastname: Keleş
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D020552:Comet Assay; D000081005:Cyclohexane Monoterpenes; D004305:Dose-Response Relationship, Drug; D039821:Monoterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D009153:Mutagens / Q000008:administration & dosage / Q000494:pharmacology; D009447:Neuroblastoma / Q000188:drug therapy*; D009474:Neurons / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats
chemical_list: D000970:Antineoplastic Agents; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D009153:Mutagens; C006923:carvone
keywords: Carvone; MTT assay; N2a neuroblastoma cell line; cell viability; comet assay; neurotoxicity; oxidative status