title: Synthesis of the IGF-II-like hormone vesiculin using regioselective formation of disulfide bonds.
abstract: Diabetes mellitus, characterised by hyperglycemia and altered β-cell function, is an increasingly common disorder affecting millions of individuals world-wide. While therapeutic regimens exist to manage the condition, diabetic individuals remain prone to complications that are detrimental to both their length and quality of life. An improved understanding of the disease which may then enable development of new treatments is therefore a desirable goal. Vesiculin, a novel IGF-II-like protein was recently isolated from the secretory granules of murine β-cells, and preliminary studies indicate it is capable of signalling via the insulin receptor (IR)/insulin-like growth factor receptor 1(IGF1R) family giving it the potential to elicit both metabolic and mitogenic responses in the beta-cell. In order to facilitate further studies on this new member of the insulin-family of hormones, we undertook a chemical synthesis of the protein using regioselective disulfide bond formation.
authors.affiliation: School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland, New Zealand.
authors.forename: Geoffrey M
authors.initials: GM
authors.lastname: Williams
authors.forename: Garth J S
authors.initials: GJ
authors.lastname: Cooper
authors.forename: Kathryn
authors.initials: K
authors.lastname: Lee
authors.forename: Lynda
authors.initials: L
authors.lastname: Whiting
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Brimble
mesh_terms: D000818:Animals; D004220:Disulfides / Q000138:chemical synthesis* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006003:Glycogen / Q000096:biosynthesis / Q000737:chemistry; D007335:Insulin-Like Growth Factor II / Q000737:chemistry*; D008297:Male; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D004220:Disulfides; D009419:Nerve Tissue Proteins; C012605:vesiculin; D007335:Insulin-Like Growth Factor II; D006003:Glycogen