title: Curculigo orchioides protects cisplatin-induced cell damage.
abstract: Cisplatin is commonly used as a chemotherapeutic agent against many human cancers. However, it generates reactive oxygen species (ROS) and has serious dose-limiting side effects, including ototoxicity. The roots of Curculigo orchioides (C. orchioides) have been used to treat auditory diseases such as tinnitus and hearing loss in Chinese traditional medicine. In the present study, we investigated the protective effects of an ethanol extract obtained from C. orchioides rhizome (COR) on cisplatin-induced cell damage in auditory cells (HEI-OC1). COR (2.5-25 μg/ml) inhibited cisplatin-induced HEI-OC1 cell damage in a dose-dependent manner. To investigate the protective mechanism of COR on cisplatin cytotoxicity in HEI-OC1 cells, we measured the effects of COR on ROS generation and lipid peroxidation in cisplatin-treated cells as well as its scavenging activities against superoxide radicals, hydroxyl radicals, hydrogen peroxide, and DPPH radicals. COR (1-25 μg/ml) had scavenging activities against superoxide radicals, hydroxyl radicals, hydrogen peroxide, and DPPH radicals, as well as reduced lipid peroxidation. In in vivo experiments, COR was shown to reduce cochlear and peripheral auditory function impairments through cisplatin-induced auditory damage in mice. These results indicate that COR protects from cisplatin-induced auditory damage by inhibiting lipid peroxidation and scavenging activities against free radicals.
authors.affiliation: College of Life Sciences, Kyung Hee University, Gyeonggi 446-701, South Korea.
authors.forename: Tong Ho
authors.initials: TH
authors.lastname: Kang
authors.forename: Bin Na
authors.initials: BN
authors.lastname: Hong
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Jung
authors.forename: Jeong-Han
authors.initials: JH
authors.lastname: Lee
authors.forename: Hong-Seob
authors.initials: HS
authors.lastname: So
authors.forename: Raekil
authors.initials: R
authors.lastname: Park
authors.forename: Yong-Ouk
authors.initials: YO
authors.lastname: You
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002945:Cisplatin / Q000633:toxicity*; D031408:Curculigo / Q000737:chemistry*; D006198:Hair Cells, Auditory / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D002945:Cisplatin