title: The effect of two differentially expressed wheat VRN-B1 alleles on the heading time is associated with structural variation in the first intron.
abstract: We measured the level of VRN-B1 transcripts within near-isogenic lines of Triticum aestivum cultivar 'Bezostaya 1' carrying two VRN-B1 alleles from cultivars 'Saratovskaya 29' and 'Diamant 2' (VRN-B1c and VRN-B1a, respectively). Both lines have similar kinetics of VRN-B1 transcript accumulation at the third to fifth leaf stages. However, quantitative PCR analysis at the third leaf stage showed that the VRN-B1c allele was transcribed 10 times faster than the VRN-B1a allele. The F1 hybrid between the lines is more similar to the line carrying the VRN-B1a allele with regard to the level of VRN-B1 transcription. The difference in the transcript levels of the VRN-B1 alleles appears to be due to the previously identified structural changes in the first intron of VRN-B1c (deletion of 0.8 kb and duplication of 0.4 kb) as compared with VRN-B1a, as no other differences were revealed in the present study. We suggested that the first intron structural changes in the VRN-B1c allele as compared with VRN-B1a result in a higher level of VRN-B1 transcripts and an earlier heading time. We confirmed that the expression of the dominant VRN-1 gene induces transcription of the recessive homoeoalleles, coupled with the reduction of the transcript level of a flowering repressor VRN-2.
authors.affiliation: Institute of Cytology and Genetics, Lavrentiev Ave. 10, Novosibirsk 630090, Russia. atos@bionet.nsc.ru
authors.forename: A B
authors.initials: AB
authors.lastname: Shcherban
authors.forename: E K
authors.initials: EK
authors.lastname: Khlestkina
authors.forename: T T
authors.initials: TT
authors.lastname: Efremova
authors.forename: E A
authors.initials: EA
authors.lastname: Salina
mesh_terms: D000483:Alleles; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D005799:Genes, Dominant; D017343:Genes, Plant; D014644:Genetic Variation; D007438:Introns; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
references.citation: Science. 2011 Jan 7;331(6013):76-9
references.pmid: 21127216
references.citation: Theor Appl Genet. 2004 Nov;109(8):1677-86
references.pmid: 15480533
references.citation: Mol Genet Genomics. 2005 Mar;273(1):54-65
references.pmid: 15690172
references.citation: Trends Plant Sci. 2007 Aug;12(8):352-7
references.pmid: 17629542
references.citation: Plant Physiol. 2008 May;147(1):355-66
references.pmid: 18359843
references.citation: Proc Natl Acad Sci U S A. 2009 May 19;106(20):8386-91
references.pmid: 19416817
references.citation: Mol Genet Genomics. 2007 Mar;277(3):249-61
references.pmid: 17151889
references.citation: Genes Genet Syst. 2007 Apr;82(2):167-70
references.pmid: 17507783
references.citation: J Hered. 2009 May-Jun;100(3):355-64
references.pmid: 19251764
references.citation: Genome. 2005 Aug;48(4):747-54
references.pmid: 16094442
references.citation: Plant Physiol. 2006 Apr;140(4):1397-405
references.pmid: 16500994
references.citation: Plant Mol Biol. 2006 Mar;60(4):469-80
references.pmid: 16525885
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):16831-6
references.pmid: 18854416
references.citation: Proc Natl Acad Sci U S A. 2003 May 13;100(10):6263-8
references.pmid: 12730378
references.citation: Plant Cell Physiol. 2003 Dec;44(12):1255-65
references.pmid: 14701921
references.citation: Plant Physiol. 2005 Aug;138(4):2364-73
references.pmid: 16055679
references.citation: Science. 2004 Mar 12;303(5664):1640-4
references.pmid: 15016992
references.citation: Plant Mol Biol. 2005 Oct;59(3):449-67
references.pmid: 16235110