title: Self-assembled nanoparticles based on amphiphilic chitosan derivative and hyaluronic acid for gene delivery.
abstract: The present work described nanoparticles (NPs) made of oleoyl-carboxymethy-chitosan (OCMCS)/hyaluronic acid (HA) using coacervation process as novel potential carriers for gene delivery. An N/P ratio of 5 and OCMCS/HA weight ratio of 4 were the optimal conditions leading to the smallest (164.94 nm), positive charged (+14.2 mV) and monodispersed NPs. OCMCS-HA/DNA (OHD) NPs showed higher in vitro DNA release rates and increased cellular uptake by Caco-2 cells due to the HA involved in NPs. The MTT survival assay indicated no significant cytotoxicity. The transfection efficiency of OHD NPs was 5-fold higher than OCMCS/DNA (OD) NPs; however, it decreased significantly in the presence of excess free HA. The results indicated that OHD NPs internalized in Caco-2 cells were mediated by the hyaluronan receptor CD44. The data obtained in the present research gave evidence of the potential of OHD NPs for the targeting and further transfer of genes to the epithelial cells.
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao, 266003, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Kong
authors.forename: Xiao Jie
authors.initials: XJ
authors.lastname: Cheng
authors.forename: Qian Qian
authors.initials: QQ
authors.lastname: Wang
authors.forename: Li Ming
authors.initials: LM
authors.lastname: Jiang
authors.forename: Xi Guang
authors.initials: XG
authors.lastname: Chen
mesh_terms: D018938:Caco-2 Cells; D049109:Cell Proliferation; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000166:cytology; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D006820:Hyaluronic Acid; D004247:DNA; D048271:Chitosan