title: Efficient embryo transfer in the common marmoset monkey (Callithrix jacchus) with a reduced transfer volume: a non-surgical approach with cryopreserved late-stage embryos.
abstract: Among primates, the common marmoset is suitable for primate embryology research. Its small body size, however, has delayed the technical development of efficient embryo transfer. Furthermore, three factors have been determined to adversely affect the performance of marmoset embryo transfer: nonsurgical approaches, the use of cryopreserved embryos, and the use of late-stage embryos. Here we performed embryo transfer under conditions that included the above three factors and using either a small (1 μl or less) or a large volume (2-3 μl) of medium. The pregnancy and birth rates were 50% (5/10) and 27% (3/11), respectively, when using the large volume, and 80% (8/10) and 75% (9/12), respectively, when using the small volume. The latter scores exceed those of previous reports using comparable conditions. Thus, it appears that these three previously considered factors could be overcome, and we propose that reducing the transfer volume to 1 μl or less is essential for successful marmoset embryo transfer.
authors.affiliation: Department of Neurophysiology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. ishiba@ncnp.go.jp
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ishibashi
authors.forename: Hideyuki H
authors.initials: HH
authors.lastname: Motohashi
authors.forename: Mami
authors.initials: M
authors.lastname: Kumon
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Hironori
authors.initials: H
authors.lastname: Okada
authors.forename: Takashi
authors.initials: T
authors.lastname: Okada
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Seki
mesh_terms: D000818:Animals; D002144:Callithrix / Q000196:embryology*; D015925:Cryopreservation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D008098:Litter Size; D011247:Pregnancy
keywords: birth weight; cryopreservation; double embryo transfer; endometrium; litter size