title: Paracoccus siganidrum sp. nov., isolated from fish gastrointestinal tract.
abstract: A bacterial strain, designated M26(T), was isolated from a fish gastrointestinal tract, collected from Zhanjiang Port, South China. 16S rRNA gene sequence analysis indicated that strain M26(T) belongs to the subclass α-Proteobacteria, being related to the genus Paracoccus, and sharing highest sequence similarity with Paracoccus alcaliphilus JCM 7364(T) (98.1 %), Paracoccus huijuniae FLN-7(T) (97.3 %), Paracoccus stylophorae KTW-16(T) (97.1 %) and Paracoccus seriniphilus DSM 14827(T) (96.9 %). The major quinone was determined to be ubiquinone Q-10, with Q-9 and Q-8 as minor components. The major fatty acid was identified as C18:1ω7c, with smaller amounts of C18:0 and C16:0. The G+C content of the genomic DNA was determined to be 64.3 mol%. The DNA hybridization value between strain M26(T) and the most closely related type strain, P. alcaliphilus, was 29.0 ± 1.0 %. The results of physiological and biochemical tests and low DNA-DNA relatedness showed that the strain could be readily distinguished from closely related species. On the basis of these phenotypic and genotypic data, strain M26(T) is concluded to represent a novel species of the genus Paracoccus, for which the name Paracoccus siganidrum sp. nov. is proposed. The type strain is M26(T) (=CCTCC AB 2012865(T) = DSM 26381(T)).
authors.affiliation: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China. liuyingxk@sina.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing-yi
authors.initials: QY
authors.lastname: Xie
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Ya-mei
authors.initials: YM
authors.lastname: Zhao
authors.forename: Yi-li
authors.initials: YL
authors.lastname: Tang
authors.forename: Jun-ying
authors.initials: JY
authors.lastname: An
authors.forename: Ping-ping
authors.initials: PP
authors.lastname: Zhu
authors.forename: Chun-hou
authors.initials: CH
authors.lastname: Xu
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005399:Fishes / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010230:Paracoccus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S