title: Glycosylated aniline polymer sensor: amine to imine conversion on protein-carbohydrate binding.
abstract: In this report, functionalized mannosylated aniline polymer (manno-PANI) was investigated as an electrochemical platform to study carbohydrate-protein interactions by exploiting the conductivity change of manno-PANI when the specific lectin binding occurs. A systematic study was performed to characterize the interconversion of polyaniline content (from amine to imine) in manno-PANI by UV-vis spectroscopy during its binding with concanavalin A (Con A). Both X-ray photoelectron spectrometry (XPS) and UV-vis results suggest that Con A binding with the manno-PANI film triggers the switching of amine functionalities in the polyaniline backbone, converting them to imine forms. Electrochemical impedance spectroscopy (EIS) was used to quantify the specific interactions between Con A and mannose by measuring the impedance change of manno-PANI film for the detection of Con A. A linear relationship between the impedance and Con A concentration was obtained, and the detection limit reaches to 0.12 nM Con A in a buffer solution (pH=7.4), whereas the addition of nonspecific control lectins to the same manno-PANI film gave very little impedance variations. Stability characterization of the manno-PANI film over 20 weeks shows a maximum drift of only 3% from the original signal. Thus, the uniquely constructed carbohydrate-PANI hybrid is a promising new carbohydrate recognition moiety for studying carbohydrate-protein interactions, presumably leading to a new electrochemical method for characterization of carbohydrate-protein interactions and carbohydrate-mediated intercellular recognitions.
authors.affiliation: Department of Chemistry, Oakland University, Rochester, MI 48309, United States.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Sun
authors.forename: Giri
authors.initials: G
authors.lastname: Vegesna
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jinghong
authors.initials: J
authors.lastname: Li
authors.forename: Xiangqun
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000588:Amines / Q000378:metabolism; D000814:Aniline Compounds / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000032:analysis / Q000378:metabolism*; D058266:Dielectric Spectroscopy / Q000379:methods; D006031:Glycosylation; D007097:Imines / Q000378:metabolism; D057230:Limit of Detection; D008358:Mannose / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000588:Amines; D000814:Aniline Compounds; D007097:Imines; C416807:polyaniline; D003208:Concanavalin A; D008358:Mannose
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