title: Avidin adsorption on silicone elastomer: effects of stretching and polyelectrolyte coatings.
abstract: Zeta potential and avidin adsorption of silicone elastomer (SE) and SE coated with polyethyleneimine (PEI) and heparin is studied under controlled hydrodynamic conditions. Zeta potential (ζ) of SE depends on the duration of contact with buffer (0.02M MOPS-KCl, pH 7.2): ζ descends from 12mV to a plateau value of -32mV during 5h and decreases further if SE is stretched (30% elongation). The time variation of ζ is attributed to surface charging of the core polymer PDMS. Coating the negatively charged SE with PEI results in ζ=42mV; a layer of heparin on top of PEI yields ζ=-46mV. On the negatively charged surfaces, avidin adsorption levels off at 5mV. Desorption of avidin from SE is negligible even when SE with adsorbed avidin is stretched, or when the stretching force is removed after adsorption of avidin. The results can be employed for designing biomaterials with properties such as surface charge and protein adsorption adjustable by stretching; the strong attachment of avidin can allow for further surface modification.
authors.affiliation: European Membrane Institute, UMR 5635 (CNRS, ENSCM, UM2), Université Montpellier 2, CC047, 2 Place Eugène Bataillon, F-34095 Montpellier Cedex 5, France. elena202@yahoo.com
authors.forename: Elena N
authors.initials: EN
authors.lastname: Vasina
authors.forename: Philippe
authors.initials: P
authors.lastname: Déjardin
mesh_terms: D000327:Adsorption; D001360:Avidin / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018625:Microscopy, Atomic Force; D011094:Polyethyleneimine / Q000737:chemistry*; D012826:Silicone Elastomers / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D012826:Silicone Elastomers; D001360:Avidin; D011094:Polyethyleneimine; D006493:Heparin