title: Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes.
abstract: The aim of this study was to develop a robust method for the simultaneous determination of the activities of three porcine CYP450 enzymes in hepatic microsomes. A cocktail consisting of three selective CYP450 probe substrates, 7-ethoxyresorufin (CYP1A), coumarin (CYP2A) and 7-benzyloxy-4-trifluoromethylcoumarin (BFC; CYP3A), was incubated with porcine liver microsomes. The presence of 7-ethoxyresorufin appears to significantly influence the kinetics of coumarin hydroxylation and BFC O-debenzylation. These results indicate that the use of 7-ethoxyresorufin in substrate cocktails together with coumarin and BFC should be avoided.
authors.affiliation: Department of Food Science, BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Monika
authors.initials: M
authors.lastname: Johansson
authors.forename: Jana
authors.initials: J
authors.lastname: Tomankova
authors.forename: Shengjie
authors.initials: S
authors.lastname: Li
authors.forename: Galia
authors.initials: G
authors.lastname: Zamaratskaia
keywords: HPLC; cytochrome P450; hepatic microsomes; multiple probe substrates
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