title: A choline derivate-modified nanoprobe for glioma diagnosis using MRI.
abstract: Gadolinium (Gd) chelate contrast-enhanced magnetic resonance imaging (MRI) is a preferred method of glioma detection and preoperative localisation because it offers high spatial resolution and non-invasive deep tissue penetration. Gd-based contrast agents, such as Gd-diethyltriaminepentaacetic acid (DTPA-Gd, Magnevist), are widely used clinically for tumor diagnosis. However, the Gd-based MRI approach is limited for patients with glioma who have an uncompromised blood-brain barrier (BBB). Moreover, the rapid renal clearance and non-specificity of such contrast agents further hinders their prevalence. We present a choline derivate (CD)-modified nanoprobe with BBB permeability, glioma specificity and a long blood half-life. Specific accumulation of the nanoprobe in gliomas and subsequent MRI contrast enhancement are demonstrated in vitro in U87 MG cells and in vivo in a xenograft nude model. BBB and glioma dual targeting by this nanoprobe may facilitate precise detection of gliomas with an uncompromised BBB and may offer better preoperative and intraoperative tumor localization.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.forename: Shixian
authors.initials: S
authors.lastname: Huang
authors.forename: Kun
authors.initials: K
authors.lastname: Shao
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Sai
authors.initials: S
authors.lastname: An
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Kuang
authors.forename: Yubo
authors.initials: Y
authors.lastname: Guo
authors.forename: Haojun
authors.initials: H
authors.lastname: Ma
authors.forename: Xuxia
authors.initials: X
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D002794:Choline / Q000737:chemistry; D003287:Contrast Media / Q000138:chemical synthesis; D005910:Glioma / Q000473:pathology*; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D003287:Contrast Media; D002794:Choline
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