title: NeuroGPS: automated localization of neurons for brain circuits using L1 minimization model.
abstract: Drawing the map of neuronal circuits at microscopic resolution is important to explain how brain works. Recent progresses in fluorescence labeling and imaging techniques have enabled measuring the whole brain of a rodent like a mouse at submicron-resolution. Considering the huge volume of such datasets, automatic tracing and reconstruct the neuronal connections from the image stacks is essential to form the large scale circuits. However, the first step among which, automated location the soma across different brain areas remains a challenge. Here, we addressed this problem by introducing L1 minimization model. We developed a fully automated system, NeuronGlobalPositionSystem (NeuroGPS) that is robust to the broad diversity of shape, size and density of the neurons in a mouse brain. This method allows locating the neurons across different brain areas without human intervention. We believe this method would facilitate the analysis of the neuronal circuits for brain function and disease studies.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China.
authors.forename: Tingwei
authors.initials: T
authors.lastname: Quan
authors.forename: Ting
authors.initials: T
authors.lastname: Zheng
authors.forename: Zhongqing
authors.initials: Z
authors.lastname: Yang
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Ding
authors.forename: Shiwei
authors.initials: S
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Hang
authors.initials: H
authors.lastname: Zhou
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D001921:Brain / Q000166:cytology*; D003198:Computer Simulation; D063132:Connectome / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D008953:Models, Anatomic; D008959:Models, Neurological; D009474:Neurons / Q000166:cytology*; D010363:Pattern Recognition, Automated / Q000379:methods*; D013569:Synapses / Q000648:ultrastructure*
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