title: Deregulated systemic IL-10/IL-12 balance in advanced and poor prognosis paediatric soft tissue sarcomas.
abstract: BACKGROUND
  The roles of interleukin 10 (IL-10) and IL-12 in regulation of cancer growth and Th1/Th2 immune responses towards cancer are unclear.
  
  
  OBJECTIVE
  To establish the prognostic significance of serum IL-10 and IL-12 in paediatric soft tissue sarcomas (STS).
  
  
  METHODS
  ELISA determinations of cytokines were performed as pre-treatment in 59 children with STS and 30 healthy controls.
  
  
  RESULTS
  Elevated IL-10 and decreased IL-12 serum levels correlated with advanced disease, poor response to chemotherapy and poor outcome. IL-10 ≥ 9.5 pg/ml, IL-12 ≤ 65 pg/ml and lymph nodes involvement independently predicted poor overall survival (OS) in multivariate Cox analysis.
  
  
  CONCLUSIONS
  Serum IL-10/IL-12 balance determination may facilitate to assess risk groups and prognosis in childhood STS.
authors.affiliation: Department of Paediatrics, Haematology, Oncology and Endocrinology, Medical University of Gdansk, Gdansk, Poland. ewabien1@wp.pl
authors.forename: Ewa
authors.initials: E
authors.lastname: Bien
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Krawczyk
authors.forename: Ewa
authors.initials: E
authors.lastname: Izycka-Swieszewska
authors.forename: Piotr
authors.initials: P
authors.lastname: Trzonkowski
authors.forename: Bernarda
authors.initials: B
authors.lastname: Kazanowska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Adamkiewicz-Drozynska
authors.forename: Anna
authors.initials: A
authors.lastname: Balcerska
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000097:blood* / Q000276:immunology; D018664:Interleukin-12 / Q000097:blood* / Q000276:immunology; D008207:Lymphatic Metastasis; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis; D018232:Rhabdomyosarcoma, Alveolar / Q000097:blood* / Q000175:diagnosis / Q000276:immunology / Q000401:mortality; D018233:Rhabdomyosarcoma, Embryonal / Q000097:blood* / Q000175:diagnosis / Q000276:immunology / Q000401:mortality; D012509:Sarcoma / Q000097:blood* / Q000175:diagnosis / Q000276:immunology / Q000401:mortality; D016019:Survival Analysis; D058408:Th1-Th2 Balance
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10; D018664:Interleukin-12