title: Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model.
abstract: Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01).
authors.affiliation: Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran. yazdani@goums.ac.ir
authors.forename: Yaghoub
authors.initials: Y
authors.lastname: Yazdani
authors.forename: Neda
authors.initials: N
authors.lastname: Keyhanvar
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Kalhor
authors.forename: Abbas
authors.initials: A
authors.lastname: Rezaei
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D002460:Cell Line; D064451:Hepcidins / Q000235:genetics / Q000378:metabolism*; D007501:Iron / Q000097:blood / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D023421:Models, Animal; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D000098742:Ferroportin
chemical_list: D027682:Cation Transport Proteins; C579599:Hamp protein, mouse; D064451:Hepcidins; D011994:Recombinant Proteins; D000098742:Ferroportin; D007501:Iron