title: Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins.
abstract: N-methylpurine DNA glycosylase (MPG), a DNA repair enzyme, functions in the DNA base excision repair (BER) pathway. Aberrant over-expression of MPG in various cancers suggests an important role of MPG in carcinogenesis. Identification of MPG-interacting proteins will help to dissect the molecular link between MPG and cancer development. In the present study, using immunoprecipitation coupled with mass spectrometry (IP/MS), we screened ubiquitin-like, containing PHD and RING finger domains 1 (UHRF1), an essential protein required for the maintenance of DNA methylation, as a MPG-interacting protein. Endogenous co-immunoprecipitation assay in cancer cells confirmed that UHRF1 interacted with MPG in a p53 status-independent manner. Confocal microscopy showed that endogenous MPG and UHRF1 were co-localized in the nucleoplasm. Furthermore, co-immunoprecipitation assay indicated that UHRF2, the homolog of UHRF1, could also interact with MPG. These results show that MPG and the UHRF family of proteins interact, thus providing a functional linkage between MPG and UHRF1/2.
authors.affiliation: Department of Cell Biology, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Song
authors.forename: Chunyan
authors.initials: C
authors.lastname: Tian
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Yin
authors.forename: Guichun
authors.initials: G
authors.lastname: Xing
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D045325:HCT116 Cells; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D061986:MCF-7 Cells; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D016601:RNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000068103:Serine-Arginine Splicing Factors; D013963:Thyroid Hormones / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D000097810:Thyroid Hormone-Binding Proteins
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins; D045647:DNA Glycosylases; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D000068103:Serine-Arginine Splicing Factors; D013963:Thyroid Hormones; D016159:Tumor Suppressor Protein p53; D044767:Ubiquitin-Protein Ligases; D000097810:Thyroid Hormone-Binding Proteins; C406364:UHRF1 protein, human; C465619:UHRF2 protein, human; C076131:DNA-3-methyladenine glycosidase II