title: Genetic regulation of human adipose microRNA expression and its consequences for metabolic traits.
abstract: The genetics of messenger RNA (mRNA) expression has been extensively studied in humans and other organisms, but little is known about genetic factors contributing to microRNA (miRNA) expression. We examined natural variation of miRNA expression in adipose tissue in a population of 200 men who have been carefully characterized for metabolic syndrome (MetSyn) phenotypes as part of the Metabolic Syndrome in Men (METSIM) study. We genotyped the subjects using high-density single-nucleotide polymorphism microarrays and quantified the mRNA abundance using genome-wide expression arrays and miRNA abundance using next-generation sequencing. We reliably quantified 356 miRNA species that were expressed in human adipose tissue, a limited number of which made up most of the expressed miRNAs. We mapped the miRNA abundance as an expression quantitative trait and determined cis regulation of expression for nine of the miRNAs and of the processing of one miRNA (miR-28). The degree of genetic variation of miRNA expression was substantially less than that of mRNAs. For the majority of the miRNAs, genetic regulation of expression was independent of the expression of mRNA from which the miRNA is transcribed. We also showed that for 108 miRNAs, mapped reads displayed widespread variation from the canonical sequence. We found a total of 24 miRNAs to be significantly associated with MetSyn traits. We suggest a regulatory role for miR-204-5p which was predicted to inhibit acetyl coenzyme A carboxylase β, a key fatty acid oxidation enzyme that has been shown to play a role in regulating body fat and insulin resistance in adipose tissue.
authors.affiliation: Department of Medicine, University of California, Los Angeles, CA 90095, USA
authors.forename: Mete
authors.initials: M
authors.lastname: Civelek
authors.forename: Raffi
authors.initials: R
authors.lastname: Hagopian
authors.forename: Calvin
authors.initials: C
authors.lastname: Pan
authors.forename: Nam
authors.initials: N
authors.lastname: Che
authors.forename: Wen-pin
authors.initials: WP
authors.lastname: Yang
authors.forename: Paul S
authors.initials: PS
authors.lastname: Kayne
authors.forename: Niyas K
authors.initials: NK
authors.lastname: Saleem
authors.forename: Henna
authors.initials: H
authors.lastname: Cederberg
authors.forename: Johanna
authors.initials: J
authors.lastname: Kuusisto
authors.forename: Peter S
authors.initials: PS
authors.lastname: Gargalovic
authors.forename: Todd G
authors.initials: TG
authors.lastname: Kirchgessner
authors.forename: Markku
authors.initials: M
authors.lastname: Laakso
authors.forename: Aldons J
authors.initials: AJ
authors.lastname: Lusis
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D006801:Humans; D024821:Metabolic Syndrome / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
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