title: Immunolocalization of meiotic proteins in Arabidopsis thaliana: method 2.
abstract: Advances in the molecular biology and genetics of Arabidopsis thaliana have led to it becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behavior during meiosis (Jones et al. Chromosome Res 11:205-215, 2003). This chapter contains a detailed description of an immunological technique currently used in our lab for the preparation of meiotic chromosomes for immunolocalization.
authors.affiliation: School of Biosciences, The University of Birmingham, Birmingham, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Armstrong
authors.forename: Kim
authors.initials: K
authors.lastname: Osman
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D032461:Chromosomes, Plant / Q000378:metabolism; D003584:Cytological Techniques; D055993:Germ Cells, Plant; D008540:Meiosis; D008853:Microscopy / Q000379:methods
chemical_list: D029681:Arabidopsis Proteins; D002868:Chromosomal Proteins, Non-Histone