title: Interactions of nanoparticles with proteins: determination of equilibrium constants.
abstract: The behavior of nanoparticles towards proteins is an important aspect across wide areas of nanotoxicology and nanomedicine. In this chapter, we describe a procedure to study the adsorption of proteins onto nanoparticle surfaces. Circular dichroism (CD) spectroscopy is utilized to quantify the amount of free protein in a solution, and the experimental information is evaluated to derive equilibrium constants for the protein adsorption/desorption equilibrium. These equilibrium constants are comparable parameters in describing the interactions between proteins and nanoparticles.
authors.affiliation: Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Lennart
authors.initials: L
authors.lastname: Treuel
authors.forename: Marcelina
authors.initials: M
authors.lastname: Malissek
mesh_terms: D002942:Circular Dichroism; D053758:Nanoparticles; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins