title: Evaluation of the NucliSENS EasyQ KPC assay for detection of Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae.
abstract: The NucliSENS EasyQ KPC assay (bioMérieux SA, Marcy l'Etoile, France) was compared with a routinely used phenotypic method for detection of Enterobacteriaceae producing Klebsiella pneumoniae carbapenemase (KPC)-type carbapenemases, using 806 stool samples and rectal swabs. Compared with the phenotypic method, the EasyQ KPC assay had a sensitivity and specificity of 93.3% and 99.0%, respectively, in this setting, with diverse KPC producers not limited to ST258 Klebsiella pneumoniae.
authors.affiliation: Manchester Royal Infirmary, Manchester, United Kingdom. ashley.mcewan@cmft.nhs.uk
authors.forename: A S
authors.initials: AS
authors.lastname: McEwan
authors.forename: A
authors.initials: A
authors.lastname: Derome
authors.forename: D
authors.initials: D
authors.lastname: Meunier
authors.forename: P J
authors.initials: PJ
authors.lastname: Burns
authors.forename: N
authors.initials: N
authors.lastname: Woodford
authors.forename: A R
authors.initials: AR
authors.lastname: Dodgson
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005602:France; D006801:Humans; D012007:Rectum / Q000382:microbiology; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
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