title: [Effect of three dental alloys on cytotoxicity and apoptosis related gene expression in L929 cells].
abstract: OBJECTIVE
  To investigate the effect of the liching liquids of 3 different kinds of dental alloys on L929 cells both at cell level and molecular level.
  
  
  METHODS
  The fibroblast L929 cells of mouse were treated in vitro with leaching liquids of 3 different kinds of dental alloys which were Au alloy, Ti and Ni-Cr alloy, respectively. The RPMI 1640 cell medium containing 10% fetal calf serum was served as a negative control and the Cu alloy was served as a positive control. The cytotoxicities of 3 dental alloys were evaluated by means of MTT and the effects of these alloys on the expression of caspase-3, caspase-8 and caspase-9 of L929 cells were examined by RT-PCR method and immunohistochemistry. The data was analyzed with SPSS13.0 software package.
  
  
  RESULTS
  The cytotoxicity of three groups were all in grade 0 after 48 hours of cultures. Caspase-8 had no change in all groups at mRNA level.The expression of caspase-3 and caspase-9 in Ni-Gr alloy group was significantly different from other groups.
  
  
  CONCLUSIONS
  It is suggested from the results that the leaching liquids of Ni-Cr alloy may induce cell apoptosis through mitochondrion pathway.
authors.affiliation: Department of Prosthodontics, School of Stomatology, Hebei United University, Tangshan 063000, Hebei Province, China. menghe10.2@163.com
authors.forename: He
authors.initials: H
authors.lastname: Meng
authors.forename: Xiu-mei
authors.initials: XM
authors.lastname: Li
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Xu
authors.forename: Rui-ying
authors.initials: RY
authors.lastname: Liang
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002858:Chromium Alloys; D003722:Dental Alloys; D005347:Fibroblasts; D015870:Gene Expression; D051379:Mice; D014025:Titanium
chemical_list: D002858:Chromium Alloys; D003722:Dental Alloys; D014025:Titanium