title: The wheat chloroplastic proteome.
abstract: With the availability of plant genome sequencing, analysis of plant proteins with mass spectrometry has become promising and admired. Determining the proteome of a cell is still a challenging assignment, which is convoluted by proteome dynamics and convolution. Chloroplast is fastidious curiosity for plant biologists due to their intricate biochemical pathways for indispensable metabolite functions. In this review, an overview on proteomic studies conducted in wheat with a special focus on subcellular proteomics of chloroplast, salt and water stress. In recent years, we and other groups have attempted to understand the photosynthesis in wheat and abiotic stress under salt imposed and water deficit during vegetative stage. Those studies provide interesting results leading to better understanding of the photosynthesis and identifying the stress-responsive proteins. Indeed, recent studies aimed at resolving the photosynthesis pathway in wheat. Proteomic analysis combining two complementary approaches such as 2-DE and shotgun methods couple to high through put mass spectrometry (LTQ-FTICR and MALDI-TOF/TOF) in order to better understand the responsible proteins in photosynthesis and abiotic stress (salt and water) in wheat chloroplast will be focused.
  
  
  CONCLUSIONS
  In this review we discussed the identification of the most abundant protein in wheat chloroplast and stress-responsive under salt and water stress in chloroplast of wheat seedlings, thus providing the proteomic view of the events during the development of this seedling under stress conditions. Chloroplast is fastidious curiosity for plant biologists due to their intricate biochemical pathways for indispensable metabolite functions. An overview on proteomic studies conducted in wheat with a special focus on subcellular proteomics of chloroplast, salt and water stress. We have attempted to understand the photosynthesis in wheat and abiotic stress under salt imposed and water deficit during seedling stage. Those studies provide interesting results leading to a better understanding of the photosynthesis and identifying the stress-responsive proteins. In reality, our studies aspired at resolving the photosynthesis pathway in wheat. Proteomic analysis united two complementary approaches such as Tricine SDS-PAGE and 2-DE methods couple to high through put mass spectrometry (LTQ-FTICR and MALDI-TOF/TOF) in order to better understand the responsible proteins in photosynthesis and abiotic stress (salt and water) in wheat chloroplast will be highlighted. This article is part of a Special Issue entitled: Translational Plant Proteomics.
authors.affiliation: Research Center for Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Abu Hena Mostafa
authors.initials: AH
authors.lastname: Kamal
authors.forename: Kun
authors.initials: K
authors.lastname: Cho
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Uozumi
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Woo
mesh_terms: D002736:Chloroplasts / Q000737:chemistry / Q000235:genetics*; D013058:Mass Spectrometry / Q000379:methods; D010788:Photosynthesis / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics; D040901:Proteomics / Q000379:methods; D036226:Seedlings / Q000737:chemistry / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D020524:Thylakoids / Q000737:chemistry; D014908:Triticum / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: FT; Fourier transform; ICR; LTQ; LTQ-FTICR-MS; MALDI; MALDI-TOF/TOF-MS; PIR; SP; Salt stress; TMD; TOF; Thylakoid; Water stress; Wheat chloroplast; cTP; chloroplast transit peptide; ion cyclotron resonance; linear quadruple trap; matrix-assisted laser desorption/ionization; protein information resources; signal peptide; time of flight; transmembrane domain