title: Effect of brominated furanones on the formation of biofilm by Escherichia coli on polyvinyl chloride materials.
abstract: To study the influence of brominated furanones on the biofilm (BF) formation by Escherichia coli (E. coli) on polyvinyl chloride (PVC) material, and to provide new ways of surface modification of materials to clinically prevent biomaterial centered infection. Three brominated furanones, dissolved in ethanol, furanone-1(3,4-dibromo-5-hydroxyl-furanone), furanone-2(4-bromo-5-(4-methoxypheny)-3-(methylamino)-furanone), and furanone-3(3,4-dibromo-5,5-dimethoxypheny-2(5H)-furanone) with representative chemical structure, were coated on the surfaces of separate PVC materials (1 × 1 cm), respectively. The surface-modified PVC materials were incubated with E. coli and for controls, 75 % ethanol-treated PVC materials were used. This treatment played as control group. The cultivation incubations were for 6, 12, 18, and 24 h. The thickness of bacterial BF and bacterial community quantity unit area on the PVC materials was determined by confocal laser scanning microscopy (CLSM), and the surface structure of bacterial BF formation was examined by scanning electron microscopy (SEM). The results of CLSM indicated the thickness of bacterial BF and bacterial community quantity unit area on PVC materials treated with furanone-3 were significantly lower than that of control at all time points (P < 0.05), whereas, the differences between furanone-1 and furanone-2 groups and control group were not significantly different (P > 0.05). The results of SEM indicated that after 6 h incubation, the quantity of bacterial attachment to the surface of PVC material treated with furanone-3 was lower than the control group. By 18 h incubation there was completely formed BF structure on the surface of control PVC material. However, there was no significant BF formation on the surface of PVC material treated with furanone-3. The impact of different brominated furanones on SA biofilm formation on the surface of PVC materials are different, furanone-3 can inhibit E. coli biofilm formation on the surface of PVC material.
authors.affiliation: Cardiothoracic Surgery Section, Lung Cancer Research Center, Yunnan Institute of Oncology, No. 3 Affiliated Hospital of Kunming Medical University, Yunnan Province Tumor Hospital, Kunming, 650118, Yunnan, China, Lhye1204@yahoo.com.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Lianhua
authors.forename: Huang
authors.initials: H
authors.lastname: Yunchao
authors.forename: Xu
authors.initials: X
authors.lastname: Geng
authors.forename: Zhou
authors.initials: Z
authors.lastname: Youquang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Guangqiang
authors.forename: Lei
authors.initials: L
authors.lastname: Yujie
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D005663:Furans / Q000737:chemistry* / Q000494:pharmacology*; D054879:Halogenation; D008855:Microscopy, Electron, Scanning; D011143:Polyvinyl Chloride / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005663:Furans; D011143:Polyvinyl Chloride
references.citation: Nature. 2002 Jan 31;415(6871):545-9
references.pmid: 11823863
references.citation: Infect Immun. 2010 Aug;78(8):3506-15
references.pmid: 20498265
references.citation: Antimicrob Agents Chemother. 2011 Jul;55(7):3510-6
references.pmid: 21576433
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2386-91
references.pmid: 16467145
references.citation: Vet Microbiol. 2009 Mar 30;135(3-4):187-95
references.pmid: 19185433
references.citation: Mol Cell. 2008 Oct 10;32(1):150-8
references.pmid: 18851841
references.citation: Clin Oral Implants Res. 2010 Feb;21(2):137-47
references.pmid: 19912272
references.citation: Mol Microbiol. 2007 Aug;65(3):780-98
references.pmid: 17608791
references.citation: Mar Biotechnol (NY). 2011 Aug;13(4):722-32
references.pmid: 21152942
references.citation: Cell Biochem Biophys. 2011 Nov;61(2):371-6
references.pmid: 21735132
references.citation: Mol Cell. 2011 Apr 22;42(2):199-209
references.pmid: 21504831
references.citation: Bioorg Med Chem Lett. 2008 Feb 1;18(3):1006-10
references.pmid: 18164977
references.citation: Planta Med. 2011 Jan;77(2):188-95
references.pmid: 20645243
references.citation: Annu Rev Microbiol. 2001;55:165-99
references.pmid: 11544353
references.citation: J Med Microbiol. 2008 Jul;57(Pt 7):904-906
references.pmid: 18566153
references.citation: Int J Artif Organs. 2010 Sep;33(9):565-7
references.pmid: 20963722