title: Identification of a novel sperm motility-stimulating protein from caprine serum: its characterization and functional significance.
abstract: OBJECTIVE
  To identify and characterize a novel sperm motility-stimulating protein (MSP) from caprine serum.
  
  
  METHODS
  Prospective experimental study.
  
  
  METHODS
  Research laboratory.
  
  
  METHODS
  Rabbits and male and female BALB/c mice.
  
  
  METHODS
  Protein purification by conventional methods and functional and immunological characterization.
  
  
  METHODS
  MSP was purified, purity was checked, and molecular weight was determined. Surface localization, tissue distribution, and IVF for contraceptive efficacy were studied.
  
  
  RESULTS
  MSP is a heat-stable 66-kDa monomeric novel protein. At 0.9 μM, it showed much higher forward motility and longer motility maintenance than other known activators. Studies with the motility analyzers CASA and SPERMA showed an increase in horizontal and vertical velocities, respectively. MSP action was cyclic AMP independent. Its occurrence was higher in testis, although blood was the richest source. MSP was localized throughout the cell surface of spermatozoa. Its antibody caused significant inhibition of sperm motility and inhibited fertilization up to 100% at 1:25 dilution, which showed its contraceptive efficacy.
  
  
  CONCLUSIONS
  MSP has high efficacy to stimulate sperm forward motility and thus may be used in biomedical application in infertility clinics, animal breeding centers, poultries, and animal conservation centers.
authors.affiliation: Sperm Biology Laboratory, Cell Biology and Physiology Division, Council of Scientific and Industrial Research-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Saha
authors.forename: Sujoy
authors.initials: S
authors.lastname: Das
authors.forename: Arpita
authors.initials: A
authors.lastname: Bhoumik
authors.forename: Prasanta
authors.initials: P
authors.lastname: Ghosh
authors.forename: Gopal Chandra
authors.initials: GC
authors.lastname: Majumder
authors.forename: Sandhya Rekha
authors.initials: SR
authors.lastname: Dungdung
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification* / Q000502:physiology*; D005260:Female; D006041:Goats / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011446:Prospective Studies; D011817:Rabbits; D013081:Sperm Motility / Q000502:physiology*
chemical_list: D001798:Blood Proteins