title: Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe.
abstract: We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Ka-Ho
authors.initials: KH
authors.lastname: Leung
authors.forename: Hong-Zhang
authors.initials: HZ
authors.lastname: He
authors.forename: Victor Pui-Yan
authors.initials: VP
authors.lastname: Ma
authors.forename: Hai-Jing
authors.initials: HJ
authors.lastname: Zhong
authors.forename: Daniel Shiu-Hin
authors.initials: DS
authors.lastname: Chan
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
authors.forename: Chung-Hang
authors.initials: CH
authors.lastname: Leung
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
mesh_terms: D001681:Biological Assay; D015374:Biosensing Techniques; D056831:Coordination Complexes / Q000737:chemistry; D004260:DNA Repair; D054856:G-Quadruplexes; D007495:Iridium / Q000737:chemistry; D049449:Luminescence; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques; D051981:Uracil-DNA Glycosidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D056831:Coordination Complexes; D007495:Iridium; D051981:Uracil-DNA Glycosidase