title: Characterization and lectin microarray of an immunomodulatory heteroglucan from Pleurotus ostreatus mycelia.
abstract: Glucans isolated from various mushroom and mycelia sources are interestingly being studied nowadays as a potent therapeutic agent. The present work was focused on the isolation, characterization and immunomodulatory study of a novel water soluble glucan from the pure mycelia of Pleurotus ostreatus. The extracted glucan was found to have a high molecular weight of ∼2.7 × 10(6)Da and mainly comprised of glucose, mannose and fucose in a ratio of 3:2:1 with both β and α linkages. Presence of terminal or interior glucose, mannose and fucose residues was also revealed using a high throughput miniaturized platform of lectin microarray. The heteroglucan folded into a triple helical conformation and exhibited enhanced immune cell activation and anti-tumor potential in tumor bearing mice model. Thus, potential biological functions incorporated in these glucan molecules acts in accord with its structural property and exploration of such structure-function relationship will unveil its diverse mechanism of action.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.forename: Bibhas
authors.initials: B
authors.lastname: Roy
authors.forename: Pradip
authors.initials: P
authors.lastname: Patra
authors.forename: Banalata
authors.initials: B
authors.lastname: Sahoo
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005643:Fucose / Q000737:chemistry; D005936:Glucans / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D005947:Glucose / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008358:Mannose / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D020076:Pleurotus / Q000378:metabolism*; D012509:Sarcoma / Q000188:drug therapy / Q000276:immunology
chemical_list: D005936:Glucans; D007155:Immunologic Factors; D037102:Lectins; D005643:Fucose; D009569:Nitric Oxide; D005947:Glucose; D008358:Mannose