title: Coupling reactions of trehalose synthase from Pyrococcus horikoshii: cost-effective synthesis and anti-adhesive activity of α-galactosyl oligosaccharides using a one-pot three-enzyme system with trehalose.
abstract: A new sugar nucleotide cycling (SNC) process was established in a one-pot three enzyme-coupled reaction using disaccharide trehalose. Trehalose synthase from Pyrococcus horikoshii could be applied to the SNC process for the synthesis of functional α-galactosyl oligosaccharides, α-galactose (Gal) epitopes and globotriose, using the effective regeneration of UDP-Gal. The α-Gal epitopes and globotriose were found to attach to the cell-surface of enteropathogenic Escherichia coli O127 (EPEC) which were bound to human Caco-2 cells. These α-galactosyl oligosaccharides were able to prohibit the attachment of EPEC, which could have resulted in colonization and disease. The α-Gal epitope III with a lactulose acceptor showed the most inhibitory activity of anti-adhesion. The results suggest that the α-galactosyl oligosaccharides may be alternative anti-adhesion molecules that overcome antibiotic resistance.
authors.affiliation: Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Soo-In
authors.initials: SI
authors.lastname: Ryu
authors.forename: Jang-Bin
authors.initials: JB
authors.lastname: Woo
authors.forename: Soo-Bok
authors.initials: SB
authors.lastname: Lee
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D001709:Biotechnology / Q000191:economics* / Q000379:methods*; D018938:Caco-2 Cells; D002417:Cattle; D003362:Cost-Benefit Analysis; D000939:Epitopes / Q000378:metabolism; D005690:Galactose / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000494:pharmacology; D043902:Pyrococcus horikoshii / Q000201:enzymology*; D014199:Trehalose / Q000378:metabolism*; D014312:Trisaccharides / Q000096:biosynthesis
chemical_list: D000939:Epitopes; D009844:Oligosaccharides; D014312:Trisaccharides; C113460:globotriose; D014199:Trehalose; D005964:Glucosyltransferases; C099692:trehalose synthase; D005690:Galactose