title: Recombinant human butyrylcholinesterase as a new-age bioscavenger drug: development of the expression system.
abstract: Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encoded bioscavenger is a vival task. Several transgenic expression systems of human BChE were developed over the past 20 years; however, none of them has been shown to make economic sense or has been approved for administration to humans. In this study, a CHO-based expression system was redesigned, resulting in a significant increase in the production level of functional recombinant human butyrylcholinesterase as compared to the hitherto existing systems. The recombinant enzyme was characterized with Elman and ELISA methods.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997.
authors.forename: D G
authors.initials: DG
authors.lastname: Ilyushin
authors.forename: O M
authors.initials: OM
authors.lastname: Haertley
authors.forename: T V
authors.initials: TV
authors.lastname: Bobik
authors.forename: O G
authors.initials: OG
authors.lastname: Shamborant
authors.forename: E A
authors.initials: EA
authors.lastname: Surina
authors.forename: V D
authors.initials: VD
authors.lastname: Knorre
authors.forename: P
authors.initials: P
authors.lastname: Masson
authors.forename: I V
authors.initials: IV
authors.lastname: Smirnov
authors.forename: A G
authors.initials: AG
authors.lastname: Gabibov
authors.forename: N A
authors.initials: NA
authors.lastname: Ponomarenko
keywords: CHO cell line; bioscavenger; butyrylcholinesterase; recombinant protein; оrganophosphorus toxins
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