title: RNA interference-mediated simultaneous silencing of four genes using cross-shaped RNA.
abstract: The structural flexibility of RNA interference (RNAi)-triggering nucleic acids suggests that the design of unconventional RNAi trigger structures with novel features is possible. Here, we report a cross-shaped RNA duplex structure, termed quadruple interfering RNA (qiRNA), with multiple target gene silencing activity. qiRNA triggers the simultaneous down-regulation of four cellular target genes via an RNAi mechanism. In addition, qiRNA shows enhanced intracellular delivery and target gene silencing over conventional siRNA when complexed with jetPEI, a linear polyethyleneimine (PEI). We also show that the long antisense strand of qiRNA is incorporated intact into an RNA-induced silencing complex (RISC). This novel RNA scaffold further expands the repertoire of RNAi-triggering molecular structures and could be used in the development of therapeutics for various diseases including viral infections and cancer.
authors.affiliation: Global Research Laboratory for RNAi Medicine, Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Tae Yeon
authors.initials: TY
authors.lastname: Lee
authors.forename: Chan Il
authors.initials: CI
authors.lastname: Chang
authors.forename: Dooyoung
authors.initials: D
authors.lastname: Lee
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Hong
authors.forename: Chanseok
authors.initials: C
authors.lastname: Shin
authors.forename: Chiang J
authors.initials: CJ
authors.lastname: Li
authors.forename: Soyoun
authors.initials: S
authors.lastname: Kim
authors.forename: Dirk
authors.initials: D
authors.lastname: Haussecker
authors.forename: Dong-Ki
authors.initials: DK
authors.lastname: Lee
mesh_terms: D020868:Gene Silencing; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D034622:RNA Interference; D014162:Transfection
chemical_list: D012313:RNA
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