title: A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo.
abstract: The integrin family of proteins has been shown to be involved in the malignant behavior of cells. We report here development of a color-coded imaging model that can visualize the interaction between αv integrin linked to green fluorescent protein (GFP) in osteosarcoma cells and blood vessels in Gelfoam® vascularized after implantation in red fluorescent protein (RFP) transgenic nude mice. Human 143B osteosarcoma cells expressing αv integrin-GFP were generated by transfection with an αv integrin-GFP vector. Gelfoam® (5×5 mm) was transplanted subcutaneously in transgenic RFP nude mice. The implanted Gelfoam® became highly vascularized with RFP vessels within 14 days. Skin flaps were made at days 7, 14, 21, 28 after transplantation of Gelfoam® for observing vascularization of the Gelfoam® using fluorescence imaging. Gelfoam® is a useful tool to observe angiogenesis in vivo. 143B cells (5 × 10(5)) expressing αv integrin-GFP were injected into the Gelfoam® seven days after transplantation of Gelfoam®. Seven days after cancer-cell injection, cancer cells and blood vessels were observed in the Gelfoam® by color-coded confocal microscopy via the skin flap. The 143B cells expressing αv integrin-GFP proliferated into the Gelfoam®, which contained RFP-expressing blood vessels. Strong expression of αv integrin-GFP in 143B cells was observed near RFP vessels in the Gelfoam®. The observation of the behavior of αv integrin-GFP and blood vessels will allow further understanding of the role of αv integrin in cancer cells.
authors.affiliation: AntiCancer, Inc, San Diego, CA 92111, USA. all@anticancer.com
authors.forename: Fuminari
authors.initials: F
authors.lastname: Uehara
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Tome
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.forename: Shinji
authors.initials: S
authors.lastname: Miwa
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Hiroshima
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.forename: Hiroki
authors.initials: H
authors.lastname: Maehara
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kanaya
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D003952:Diagnostic Imaging; D005260:Female; D005781:Gelatin Sponge, Absorbable; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D039564:Integrin alphaV / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D012516:Osteosarcoma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D039564:Integrin alphaV; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein