title: Kinesin 5B (KIF5B) is required for progression through female meiosis and proper chromosomal segregation in mitotic cells.
abstract: The fidelity of chromosomal segregation during cell division is important to maintain chromosomal stability in order to prevent cancer and birth defects. Although several spindle-associated molecular motors have been shown to be essential for cell division, only a few chromosome arm-associated motors have been described. Here, we investigated the role of Kinesin 5b (Kif5b) during female mouse meiotic cell development and mitotic cell division. RNA interference (RNAi)-mediated silencing of Kif5b in mouse oocytes induced significant delay in germinal vesicle breakdown (GVBD) and failure in extrusion of the first polar body (PBE). In mitotic cells, knockdown of Kif5b leads to centrosome amplification and a chromosomal segregation defect. These data suggest that KIF5B is critical in suppressing chromosomal instability at the early stages of female meiotic cell development and mitotic cell division.
authors.affiliation: Departments of Therapeutic Radiology and Genetics and The Yale Comprehensive Cancer Center, New Haven, CT, USA.
authors.forename: Dawit
authors.initials: D
authors.lastname: Kidane
authors.forename: Denny
authors.initials: D
authors.lastname: Sakkas
authors.forename: Timothy
authors.initials: T
authors.lastname: Nottoli
authors.forename: James
authors.initials: J
authors.lastname: McGrath
authors.forename: Joann B
authors.initials: JB
authors.lastname: Sweasy
mesh_terms: D000818:Animals; D018385:Centrosome / Q000378:metabolism* / Q000473:pathology; D043171:Chromosomal Instability; D020090:Chromosome Segregation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D016547:Kinesins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008540:Meiosis / Q000235:genetics; D051379:Mice; D008938:Mitosis / Q000235:genetics; D009865:Oocytes / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D008941:Spindle Apparatus / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D034741:RNA, Small Interfering; C475469:Kif5b protein, mouse; D016547:Kinesins
references.citation: J Cell Biol. 1994 Oct;127(1):187-201
references.pmid: 7929562
references.citation: Cell Motil Cytoskeleton. 1997;36(3):291-302
references.pmid: 9067624
references.citation: J Cell Biol. 2007 Jan 1;176(1):65-75
references.pmid: 17190794
references.citation: Development. 1997 Jun;124(12):2365-76
references.pmid: 9199363
references.citation: Nature. 1997 Apr 10;386(6625):623-7
references.pmid: 9121588
references.citation: Nature. 2007 Nov 29;450(7170):745-9
references.pmid: 18046416
references.citation: Curr Opin Genet Dev. 2007 Apr;17(2):157-62
references.pmid: 17324569
references.citation: Neuron. 2004 Aug 19;43(4):513-25
references.pmid: 15312650
references.citation: Cell. 1998 Jun 26;93(7):1147-58
references.pmid: 9657148
references.citation: Drug Discov Today. 2003 Jun 1;8(11):494-502
references.pmid: 12818519
references.citation: Front Biosci. 2006 Jan 01;11:620-36
references.pmid: 16146756
references.citation: J Cell Biol. 1995 May;129(3):709-23
references.pmid: 7730406
references.citation: J Cell Sci. 1972 Sep;11(2):521-41
references.pmid: 5076360
references.citation: Cell. 1986 May 9;45(3):329-42
references.pmid: 3516413
references.citation: Nature. 2002 May 2;417(6884):83-7
references.pmid: 11986669
references.citation: Chromosoma. 2006 Jun;115(3):241-9
references.pmid: 16547726
references.citation: Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7004-11
references.pmid: 11416179
references.citation: Science. 2004 Mar 5;303(5663):1519-22
references.pmid: 15001780
references.citation: Trends Cell Biol. 2005 Jul;15(7):371-7
references.pmid: 15935670
references.citation: Trends Cell Biol. 2002 Jan;12(1):9
references.pmid: 11853996
references.citation: Dev Cell. 2004 Nov;7(5):637-51
references.pmid: 15525526
references.citation: J Cell Sci. 2002 Mar 1;115(Pt 5):931-40
references.pmid: 11870212
references.citation: Biochem J. 2001 Dec 15;360(Pt 3):549-56
references.pmid: 11736643
references.citation: J Cell Biol. 2003 Sep 15;162(6):1003-16
references.pmid: 12975346
references.citation: Nat Rev Genet. 2001 Apr;2(4):280-91
references.pmid: 11283700
references.citation: Nature. 2003 Apr 17;422(6933):746-52
references.pmid: 12700768
references.citation: J Cell Biol. 1977 Jun;73(3):672-84
references.pmid: 873994
references.citation: Nat Genet. 2004 Feb;36(2):172-7
references.pmid: 14730302
references.citation: Biol Cell. 2005 Jun;97(6):425-34
references.pmid: 15898952
references.citation: J Cell Biol. 2004 Aug 30;166(5):613-20
references.pmid: 15326200
references.citation: Nat Med. 2012 Feb 12;18(3):375-7
references.pmid: 22327624
references.citation: Mol Cell Biol. 2006 May;26(10):3853-63
references.pmid: 16648480
references.citation: Nature. 2004 Jan 22;427(6972):364-70
references.pmid: 14681690
references.citation: Mol Biol Cell. 2004 Jan;15(1):219-33
references.pmid: 14528012
references.citation: Int Rev Cytol. 2008;265:111-58
references.pmid: 18275887
references.citation: J Cell Biol. 2008 Feb 25;180(4):665-72
references.pmid: 18283116
references.citation: Nat Rev Mol Cell Biol. 2007 May;8(5):379-93
references.pmid: 17426725
references.citation: J Cell Biol. 1997 Jan 27;136(2):229-40
references.pmid: 9015296
references.citation: Annu Rev Cell Dev Biol. 2002;18:193-219
references.pmid: 12142285
references.citation: Curr Biol. 2007 Nov 6;17(21):1837-46
references.pmid: 17980598