title: Paraffin sections of 70-100 μm: a novel technique and its benefits for studying the nervous system.
abstract: This technical communication provides a protocol for processing paraffin-embedded tissue blocks from the nervous system sectioned at 70-100 μm. Staining is performed on free-floating sections. Unlike 4-15 μm thick paraffin sections, this technique permits (1) the accurate recognition of specific cell types and cytoarchitectonic units (subcortical nuclei and cortical layers) and, owing to the superimposition of large numbers of biological structures, (2) the accurate assessment of pathological changes, e.g., those associated with synucleinopathies, tauopathies, and TDP-43 proteinopathies.
authors.affiliation: Clinical Neuroanatomy Section, Department of Neurology, Center for Biomedical Research, University of Ulm, Helmholtzstrasse 8/1, 89081 Ulm, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Feldengut
authors.forename: Kelly
authors.initials: K
authors.lastname: Del Tredici
authors.forename: Heiko
authors.initials: H
authors.lastname: Braak
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016229:Amyloid beta-Peptides / Q000378:metabolism; D006801:Humans; D008297:Male; D009420:Nervous System / Q000166:cytology*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D016612:Paraffin Embedding / Q000379:methods*; D013194:Staining and Labeling; D057177:TDP-43 Proteinopathies / Q000378:metabolism; D055815:Young Adult; D016875:tau Proteins / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D016875:tau Proteins