title: Phylogenetic identification of marine bacteria isolated from deep-sea sediments of the eastern South Atlantic Ocean.
abstract: The deep-sea environments of the South Atlantic Ocean are less studied in comparison to the North Atlantic and Pacific Oceans. With the aim of identifying the deep-sea bacteria in this less known ocean, 70 strains were isolated from eight sediment samples (depth range between 1905 to 5560 m) collected in the eastern part of the South Atlantic, from the equatorial region to the Cape Abyssal Plain, using three different culture media. The strains were classified into three phylogenetic groups, Gammaproteobacteria, Firmicutes and Actinobacteria, by the analysis of 16s rRNA gene sequences. Gammaproteobacteria and Firmicutes were the most frequently identified groups, with Halomonas the most frequent genus among the strains. Microorganisms belonging to Firmicutes were the only ones observed in all samples. Sixteen of the 41 identified operational taxonomic units probably represent new species. The presence of potentially new species reinforces the need for new studies in the deep-sea environments of the South Atlantic.
authors.affiliation: Centro de Ciências Tecnológicas da Terra e do Mar, Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, CEP 88302202 Brazil.
authors.forename: Marcus Adonai Castro
authors.initials: MA
authors.lastname: da Silva
authors.forename: Angélica
authors.initials: A
authors.lastname: Cavalett
authors.forename: Ananda
authors.initials: A
authors.lastname: Spinner
authors.forename: Daniele Cristina
authors.initials: DC
authors.lastname: Rosa
authors.forename: Regina Beltrame
authors.initials: RB
authors.lastname: Jasper
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Quecine
authors.forename: Maria Letícia
authors.initials: ML
authors.lastname: Bonatelli
authors.forename: Aline
authors.initials: A
authors.lastname: Pizzirani-Kleiner
authors.forename: Gertrudes
authors.initials: G
authors.lastname: Corção
authors.forename: André Oliveira de Souza
authors.initials: AO
authors.lastname: Lima
keywords: Cultivable bacteria; Deep-sea sediments; Halomonas; Phylogenetic identification; South Atlantic Ocean
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