title: Transient receptor potential vanilloid-2 mediates the effects of transient heat shock on endocytosis of human monocyte-derived dendritic cells.
abstract: Our goal was to investigate the effect of heat shock on human monocyte-derived dendritic cells (DCs) and to dissect the role of thermosensitive transient receptor potential (TRP) channels in the process. We provide evidence that a short heat shock challenge (43 °C) decreased the endocytotic activity of the DCs and that this effect could be alleviated by the RNAi-mediated knockdown of TRPV2 but, importantly, not by the pharmacological (antagonists) or molecular (RNAi) suppression of TRPV1 and TRPV4 activities/levels. Likewise, the heat shock-induced robust membrane currents were selectively and markedly inhibited by TRPV2 "silencing" whereas modulation of TRPV1 and TRPV4 activities, again, had no effect. These intriguing data introduce TRPV2-coupled signaling as a key player in mediating the cellular actions of heat shock on DCs.
authors.affiliation: DE-MTA Lendület Cellular Physiology Research Group, Department of Physiology, University of Debrecen, Medical and Health Science Center, Research Center for Molecular Medicine, Debrecen, Hungary.
authors.forename: Attila Gábor
authors.initials: AG
authors.lastname: Szöllősi
authors.forename: Attila
authors.initials: A
authors.lastname: Oláh
authors.forename: István Balázs
authors.initials: IB
authors.lastname: Tóth
authors.forename: Ferenc
authors.initials: F
authors.lastname: Papp
authors.forename: Gabriella
authors.initials: G
authors.lastname: Czifra
authors.forename: György
authors.initials: G
authors.lastname: Panyi
authors.forename: Tamás
authors.initials: T
authors.lastname: Bíró
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism*; D055724:Electrophysiological Phenomena; D004705:Endocytosis; D005786:Gene Expression Regulation; D018869:Heat-Shock Response; D006801:Humans; D009000:Monocytes / Q000166:cytology*; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; C400769:TRPV2 protein, human; C416986:TRPV4 protein, human