title: Succinic acid production by Actinobacillus succinogenes NJ113 using corn steep liquor powder as nitrogen source.
abstract: In this study, corn steep liquor powder (CSL) was used as nitrogen source to replace the relatively costly yeast extract typically used for the production of succinic acid with Actinobacillus succinogenes NJ113. Moreover, when heme was added to the fermentation medium and the culture was agitated at a low speed, a maximum succinic acid concentration of 37.9 g/l was obtained from a glucose concentration of 50 g/l, and a productivity of 0.75 g/l/h was achieved. These yields are almost as high as for fermentation with glucose and yeast extract. These results suggest that heme-supplemented CSL may be a suitable alternative nitrogen source for a cost-effective method of producing succinic acid with A. succinogenes NJ113 while consuming less energy than previous methods.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, China.
authors.forename: Yong-lan
authors.initials: YL
authors.lastname: Xi
authors.forename: Ke-quan
authors.initials: KQ
authors.lastname: Chen
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Dai
authors.forename: Jiang-feng
authors.initials: JF
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Ping-Kai
authors.initials: PK
authors.lastname: Ouyang
mesh_terms: D000188:Actinobacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006418:Heme / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism* / Q000494:pharmacology; D011208:Powders; D019802:Succinic Acid / Q000378:metabolism*; D013997:Time Factors; D014866:Waste Products; D003313:Zea mays / Q000737:chemistry*
chemical_list: D003470:Culture Media; D011208:Powders; D014866:Waste Products; D006418:Heme; D019802:Succinic Acid; D009584:Nitrogen