title: Bioimaging application of highly luminescent silica-coated ZnO-nanoparticle quantum dots with biotin.
abstract: We synthesized ZnO-nanoparticle quantum dots (QDs) as a fluorescent probe for biological applications. Highly luminescent silica-coated ZnO-nanoparticle QDs dispersed in an aqueous medium were synthesized using the sol-gel process. The ZnO-nanoparticle QDs were coated with silica to improve the water stability of the ZnO nanoparticles. NH2 groups were introduced on the surface of the silica-coated ZnO-nanoparticle QDs first by the addition of 3-aminopropyltrimethoxysilane and then by biotinylation with sulfosuccinimidyl-6-(biotin-amido) hexanoate (sulfo-NHS-LC-bioton). We demonstrated that avidin-immobilized agarose beads were tagged by the silica-coated ZnO-nanoparticle QDs with biotin by the selective avidin-biotin interaction, furnishing a fluorescent image upon excitation with UV light. Furthermore, use of the silica-coated ZnO-nanoparticle QDs with biotin in cell-labeling applications was attempted, and attachment of the silica-coated ZnO-nanoparticle QDs with biotin to nerve cells and actin filaments was achieved.
authors.affiliation: Department of Biochemistry and Applied Chemistry, Kurume National College of Technology, Kurume, Fukuoka, Japan. mtym@kurume-nct.ac.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsuyama
authors.forename: Neil
authors.initials: N
authors.lastname: Ihsan
authors.forename: Keiichi
authors.initials: K
authors.lastname: Irie
authors.forename: Kenichi
authors.initials: K
authors.lastname: Mishima
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Okuyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Muto
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D001710:Biotin / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D015034:Zinc Oxide / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001710:Biotin; D012822:Silicon Dioxide; D015034:Zinc Oxide