title: Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae.
abstract: The effector protein MoHrip1 from the pathogenic fungus Magnaporthe oryzae was purified and crystallized using the sitting-drop vapour-diffusion method. Native crystals appeared in a solution composed of 0.005 M cobalt(II) chloride hexahydrate, 0.005 M nickel(II) chloride hexahydrate, 0.005 M cadmium chloride hydrate, 0.005 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 12%(w/v) polyethylene glycol 3350. A native data set was collected to 1.9 Å resolution at 100 K using an in-house X-ray source. The structure of MoHrip1 was successfully determined by molecular replacement using a homologous structure.
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, People's Republic of China.
authors.forename: Caizhi
authors.initials: C
authors.lastname: Zhang
authors.forename: Xinqi
authors.initials: X
authors.lastname: Liu
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zeng
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D020082:Magnaporthe / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins
keywords: Magnaporthe oryzae; MoHrip1
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