title: Genomic analysis of a newly isolated of Japanese encephalitis virus strain, CQ11-66, from a pediatric patient in China.
abstract: BACKGROUND
  Japanese encephalitis virus (JEV) is one of the major causative agents of viral encephalitis in East Asia, Southeast Asia and Australia. However, no clinical JEV strain has yet been isolated from JE patients in Chongqing, China. In this study, we report the genomic analysis of a new JEV strain, CQ11-66, isolated from a pediatric patient in Chongqing, China.
  
  
  RESULTS
  Virus isolation was carried out in BHK-21 cells. Nested PCR was used to detect and isolate the JEV strain, and computer analysis of phylogenetic relationships, nucleic acid homology studies and deduction of the amino acid sequence were conducted using ClustalX (1.8) and Mega5 software. The JEV strain CQ11-66 was isolated from patient cerebrospinal fluid. The sequenced genome of CQ11-66 was 10,863 nucleotides in length, whereas other strains, such as SX09S-01, contain 10,965 nucleotides. Sequence comparison of the CQ11-66 polyprotein open reading frame (ORF) with those of 21 other JEV strains revealed that the nucleotide sequence divergence ranged from 1.68% to 18.46%. Sequence analysis of the full-length CQ11-66 E gene sequence with those of 30 other JEV isolates also identified nucleotide divergence, ranging from 1.69% to 18.74%. Phylogenetic analyses indicated that the CQ11-66 strain belonged to genotype III.
  
  
  CONCLUSIONS
  JEV genotype III still circulates in Chongqing and it is therefore important for active surveillance of JEV genotype III to be conducted in the pediatric population.
authors.affiliation: Department of Infectious diseases and Gastroenterology, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Er Road, Yuzhong District, Chongqing 400014, PR China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Xu
authors.forename: Ruixi
authors.initials: R
authors.lastname: Liu
authors.forename: Sheng
authors.initials: S
authors.lastname: Ye
authors.forename: Hua
authors.initials: H
authors.lastname: Ling
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D000818:Animals; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D002675:Child, Preschool; D002681:China; D016000:Cluster Analysis; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000821:virology*; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral
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