title: Extracellular Matrix Protein Mindin is Required for the Complete Allergic Response to Fungal-Associated Proteinase.
abstract: Asthma remains an important cause of morbidity and mortality with an incidence that continues to rise. Despite the importance of this disease, the mechanisms by which the host develops allergic airways disease remain poorly understood. The development of allergic airways disease appears to be contingent on activation of both the innate and adaptive immune system, but little is known about the cross-talk between these two systems. The extracellular matrix protein mindin (Spondin 2) has been previously demonstrated to have functional roles in both the innate and adaptive immunological responses. Previous work supports that pulmonary challenge with fungal-associated allergenic proteinase (FAP) induces an innate allergic response. We hypothesized that mindin would modify the biological response to FAP. Saline or FAP was administered by oropharyngeal aspiration to C57BL/6 wild type or mindin-null mice every 4 days for a total of five exposures. FAP exposed C57BL/6 mice developed enhanced airway hyperresponsiveness (AHR) to methacholine challenge and increased neutrophils and eosinophils in the bronchoalveolar lavage as compared to saline exposed controls. These responses were significantly reduced in mindin-null mice exposed to FAP. FAP challenge was associated with a broad induction of cytokines (IL-1β, TNFα, Th1, Th2, and IL-17), chemokines, and growth factors, which were reduced in mindin-null mice exposed to FAP. RNA expression in lung monocytes for representative M1 and M2 activation markers were increased by FAP, but were independent of mindin. Our observations support that challenge with FAP results in activation of both innate and adaptive immune signaling pathways in a manner partially dependent on mindin. These findings suggest a potential role for the extracellular matrix protein mindin in cross-talk between the innate and adaptive immune systems.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, NC, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Tighe
authors.forename: Erin N
authors.initials: EN
authors.lastname: Potts
authors.forename: Feifei
authors.initials: F
authors.lastname: Feng
authors.forename: Zhuowei
authors.initials: Z
authors.lastname: Li
authors.forename: Benjamin
authors.initials: B
authors.lastname: Frush
authors.forename: You-Wen
authors.initials: YW
authors.lastname: He
authors.forename: David B
authors.initials: DB
authors.lastname: Corry
authors.forename: Paul W
authors.initials: PW
authors.lastname: Noble
authors.forename: John W
authors.initials: JW
authors.lastname: Hollingsworth
keywords: Allergy; Aspergillus; Asthma; Environment; Extracellular matrix; Reactive airways disease
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