title: Transcription of the tyrosinase gene family in an Atlantic salmon leukocyte cell line (SHK-1) is influenced by temperature, but not by virus infection or bacterin stimulation.
abstract: The present study was performed to address putative links between the immune and pigmentary systems. A pigment-producing leukocyte-like cell-line (SHK-1 cells) of Atlantic salmon (Salmo salar L.) was exposed to different temperatures, poly I:C, bacterin or infected with virus (infectious pancreatic necrosis virus or infectious salmon anaemia virus). The effect of this stimulation regarding the transcription-pattern of the tyrosinase gene family (melanin genes) and the immune-related genes MHC class II and IFN-1 was analysed using real-time RT-qPCR. At 10°C cultivation, tyrosinase and dopachrome tautomerase remained unregulated. At 15°C, a moderate up-regulation was induced, while at 20°C, these genes were up-regulated in an exponential manner over time. Temperature did not affect the transcription of the immune-related genes. Virus infections, poly I:C or bacterin had no influence on the transcription of the melanogenesis-related genes, but triggered the immune-related genes. Our findings revealed no connections between the pigmentary and immune systems, but demonstrated a hereto undiscovered temperature-effect on the tyrosinase gene family.
authors.affiliation: Section of Anatomy and Pathology, Department of Basic Science and Aquatic Medicine, Norwegian School of Veterinary Science, Oslo, Norway.
authors.forename: Hilde A S
authors.initials: HA
authors.lastname: Larsen
authors.forename: Lars
authors.initials: L
authors.lastname: Austbø
authors.forename: Melanie
authors.initials: M
authors.lastname: König
authors.forename: Henning
authors.initials: H
authors.lastname: Sørum
authors.forename: Espen
authors.initials: E
authors.lastname: Rimstad
authors.forename: Erling O
authors.initials: EO
authors.lastname: Koppang
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology; D002460:Cell Line; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D018061:Infectious pancreatic necrosis virus / Q000276:immunology; D007370:Interferon Type I / Q000235:genetics / Q000276:immunology; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000276:immunology; D046829:Isavirus / Q000276:immunology; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000276:immunology*; D010860:Pigments, Biological / Q000235:genetics / Q000276:immunology*; D011070:Poly I-C / Q000494:pharmacology; D020317:Salmo salar / Q000235:genetics / Q000276:immunology*; D013696:Temperature; D014158:Transcription, Genetic / Q000187:drug effects / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D029941:Fish Proteins; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I; D010860:Pigments, Biological; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase; D011070:Poly I-C