title: Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata.
abstract: CEL-III is a Ca(2+)-dependent haemolytic lectin isolated from the marine invertebrate Cucumaria echinata. This lectin binds to Gal/GalNAc-containing carbohydrate chains on the cell surface and, after conformational changes, oligomerizes to form ion-permeable pores in cell membranes. CEL-III also forms soluble oligomers similar to those formed in cell membranes upon binding of specific carbohydrates in high-pH and high-salt solutions. These soluble and membrane CEL-III oligomers were crystallized and X-ray diffraction data were collected. Crystals of soluble oligomers and membrane oligomers diffracted X-rays to 3.3 and 4.2 Å resolution, respectively, using synchrotron radiation and the former was found to belong to space group C2. Self-rotation functional analysis of the soluble oligomer crystal suggested that it might be composed of heptameric CEL-III.
authors.affiliation: Laboratory of Biomolecular Chemistry, Graduate School of Engineering, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Unno
authors.forename: Keigo
authors.initials: K
authors.lastname: Hisamatsu
authors.forename: Tomonao
authors.initials: T
authors.lastname: Nagao
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tateya
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Goda
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Hatakeyama
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D037102:Lectins / Q000737:chemistry*; D055503:Protein Multimerization; D012616:Sea Cucumbers / Q000737:chemistry*
chemical_list: C402644:CEL-III lectin, Cucumaria echinata; D037102:Lectins
keywords: Cucumaria echinata; haemolytic lectin CEL-III
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