title: A proteomic characterization of factors enriched at nascent DNA molecules.
abstract: DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance.
authors.affiliation: Genomic Instability Group, Spanish National Cancer Research Centre, Madrid 28029, Spain.
authors.forename: Andres J
authors.initials: AJ
authors.lastname: Lopez-Contreras
authors.forename: Isabel
authors.initials: I
authors.lastname: Ruppen
authors.forename: Maria
authors.initials: M
authors.lastname: Nieto-Soler
authors.forename: Matilde
authors.initials: M
authors.lastname: Murga
authors.forename: Sara
authors.initials: S
authors.lastname: Rodriguez-Acebes
authors.forename: Silvia
authors.initials: S
authors.lastname: Remeseiro
authors.forename: Sara
authors.initials: S
authors.lastname: Rodrigo-Perez
authors.forename: Ana M
authors.initials: AM
authors.lastname: Rojas
authors.forename: Juan
authors.initials: J
authors.lastname: Mendez
authors.forename: Javier
authors.initials: J
authors.lastname: Muñoz
authors.forename: Oscar
authors.initials: O
authors.lastname: Fernandez-Capetillo
mesh_terms: D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication; D053263:Gene Regulatory Networks; D057809:HEK293 Cells; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D040901:Proteomics; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D054875:Ubiquitination; D018980:Williams Syndrome / Q000235:genetics / Q000378:metabolism
chemical_list: D051741:Kruppel-Like Transcription Factors; D025841:Small Ubiquitin-Related Modifier Proteins; C457300:ZNF24 protein, human; D004247:DNA
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