title: Induction of specific immune responses in piglets by intramuscular immunization with fimbrial adhesin FaeG expressed in Lactococcus lactis.
abstract: Fimbrial adhesin plays a critical role in the pathogenesis of enterotoxigenic Escherichia coli (ETEC)-induced piglet diarrhoea. Lactococcus lactis is an attractive food-grade host for the production of heterologous antigens. We previously demonstrated that fimbrial adhesin FaeG was expressed in L. lactis and that oral immunization in mice with recombinant L. lactis expressing FaeG induced F4-specific mucosal and systemic immune responses. In the present study, we explored the immune responses of piglets induced by intramuscular vaccination with recombinant L. lactis expressing rFaeG. Intramuscular vaccination resulted in significantly elevated serum IgG level and modest increases in serum IgA and IgM levels. In addition, IgG, IgA, and IgM antibody secreting cells were induced in the spleen, mesenteric lymph nodes, and jejunum. The growth performance of piglets was not influenced by intramuscular vaccination. The results suggest that L. lactis expressing FaeG is a promising candidate vaccine against ETEC.
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, 145 Shiqiao Road, Hangzhou, Zhejiang 310021, China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Liu
authors.forename: Yongming
authors.initials: Y
authors.lastname: Li
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Xu
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology*; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D013294:Lactococcus lactis / Q000235:genetics / Q000276:immunology*; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control
chemical_list: D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; C494388:FaeG protein, E coli; D011994:Recombinant Proteins