title: Effects of lysed Enterococcus faecalis FK-23 on experimental allergic rhinitis in a murine model.
abstract: In the current study, we sought to investigate whether lysed Enterococcus faecalis FK-23 (LFK), a heat-killed probiotic preparation, attenuated eosinophil influx into the upper airway and had immunomodulatory activity in a murine allergic rhinitis model. Eighteen BALB/c mice were divided into three groups; the ovalbumin (OVA)-sensitized/challenged group, which received saline orally for 6 weeks (OVA group), the OVA-sensitized/challenged group, which received LFK orally for 6 weeks (LFK-fed group), and the non-sensitized group, which received saline for 6 weeks (saline control group). Nasal rubbing and sneezing were monitored during the study. After the final challenge, interleukin (IL)-4, interferon (IFN)-γ, and OVA-specific IgE levels in the sera and splenocyte culture supernatants were determined, eosinophilic infiltrate into the upper airway was quantified, and splenic CD4+CD25+ regulatory T cells (Tregs) were examined by flow cytometry. We found that nasal rubbing was significantly reduced in LFK-fed mice compared to the OVA group on d 27 and 35, and sneezing was significantly inhibited by LFK administration for 35 d. LFK-fed mice had significantly less eosinophil influx into the nasal mucosa than the OVA group. There were no significant differences between the LFK-fed group and OVA group in the serum and splenocyte culture supernatant levels of IL-4, IFN-γ, and OVA-specific IgE. Interestingly, the LFK-fed mice had a significantly greater percentage of splenic CD4+CD25+ Tregs than OVA group. Our results indicate that oral administration of LFK may alleviate nasal symptoms, reduce nasal eosinophilia, and increase the percentage of CD4+CD25+ Tregs in experimental allergic rhinitis.
authors.affiliation: Department of Otorhinolaryngology, the First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors.forename: Luping
authors.initials: L
authors.lastname: Zhu
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimada
authors.forename: Ruoxi
authors.initials: R
authors.lastname: Chen
authors.forename: Meiping
authors.initials: M
authors.lastname: Lu
authors.forename: Qingzhao
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wenmin
authors.initials: W
authors.lastname: Lu
authors.forename: Min
authors.initials: M
authors.lastname: Yin
authors.forename: Tadao
authors.initials: T
authors.lastname: Enomoto
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
keywords: Enterococcus faecalis; allergic rhinitis; cytokines; eosinophils; mice; probiotics; regulatory T-lymphocytes
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