title: Measurement of G protein-coupled receptor surface expression.
abstract: The quantity of G protein-coupled receptors (GPCRs) expressed on the cell surface is an important factor regulating receptor signaling. Maturation, internalization, recycling and degradation together determine the net amount of receptor surface expression. Understanding every aspect of the receptor lifecycle will facilitate the development of therapeutic applications. A number of assays for measuring the surface expression of GPCRs are currently available. This minireview summarizes the currently available assays and their suitability and usage for measuring GPCR surface expression.
authors.affiliation: Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Pieter
authors.initials: P
authors.lastname: Beerepoot
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Lam
authors.forename: Ali
authors.initials: A
authors.lastname: Salahpour
mesh_terms: D019390:Arrestins / Q000378:metabolism*; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D059748:Proteolysis; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D015398:Signal Transduction / Q000502:physiology; D013499:Surface Properties
chemical_list: D019390:Arrestins; D043562:Receptors, G-Protein-Coupled