title: Genotyping Cryptosporidium andersoni in cattle in Shaanxi Province, Northwestern China.
abstract: The present study examined the prevalence and genotypes of Cryptosporidium andersoni in cattle in Shaanxi province, China. A total of 2071 fecal samples (847 from Qinchuan cattle and 1224 from dairy cattle) were examined for the presence of Cryptosporidium oocysts, and 70 samples (3.4%) were C. andersoni-positive and those positive samples were identified by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) and the Cryptosporidium oocyst wall protein (COWP) genes. C. andersoni was the only species found in the examined cattle in this province. Fifty-seven C. andersoni isolates were characterized into 5 MLST subtypes using multilocus sequence typing analysis, including a new subtype in the native beef breed Qinchuan cattle. All of these C. andersoni isolates presented a clonal genetic structure. These findings provide new insights into the genetic structure of C. andersoni isolates in Shaanxi province and basic data of Cryptosporidium prevalence status, which in turn have implications for controlling cryptosporidiosis in this province.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhao
authors.forename: Wan-Xin
authors.initials: WX
authors.lastname: Ren
authors.forename: Man
authors.initials: M
authors.lastname: Gao
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Bian
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.forename: Mei-Mei
authors.initials: MM
authors.lastname: Cong
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.forename: Rong-Jun
authors.initials: RJ
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Qi
authors.forename: Mao-Zhen
authors.initials: MZ
authors.lastname: Qi
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
authors.forename: Long-Xian
authors.initials: LX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology; D002681:China / Q000453:epidemiology; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000235:genetics / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan; D005243:Feces / Q000469:parasitology; D005838:Genotype; D058885:Multilocus Sequence Typing; D033661:Oocysts / Q000502:physiology; D010802:Phylogeny; D015995:Prevalence; D015800:Protozoan Proteins / Q000145:classification / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000145:classification / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S; C106305:oocyst wall protein, Cryptosporidium
references.citation: Vet Parasitol. 2002 Jul 29;107(1-2):37-49
references.pmid: 12072212
references.citation: Exp Parasitol. 2010 Jan;124(1):90-7
references.pmid: 19303009
references.citation: Vet Parasitol. 2004 Jun 21;122(2):103-17
references.pmid: 15177715
references.citation: Vet Parasitol. 2006 Apr 30;137(3-4):202-9
references.pmid: 16488542
references.citation: PLoS One. 2012;7(8):e43782
references.pmid: 22937094
references.citation: FEMS Immunol Med Microbiol. 2008 Apr;52(3):309-23
references.pmid: 18205803
references.citation: J Parasitol. 2003 Apr;89(2):399-402
references.pmid: 12760666
references.citation: Parasitol Res. 2008 Apr;102(5):901-5
references.pmid: 18259778
references.citation: Vet Parasitol. 2012 May 25;186(3-4):188-95
references.pmid: 22154970
references.citation: Vet Parasitol. 2007 Mar 15;144(1-2):1-9
references.pmid: 17097231
references.citation: Appl Environ Microbiol. 2000 Dec;66(12):5499-502
references.pmid: 11097936
references.citation: Korean J Parasitol. 2004 Mar;42(1):1-6
references.pmid: 15060334
references.citation: Parasitol Int. 2011 Jan;60(1):1-4
references.pmid: 20884374
references.citation: Infect Genet Evol. 2007 Mar;7(2):197-205
references.pmid: 17010677
references.citation: Vet Parasitol. 2010 May 11;169(3-4):382-6
references.pmid: 20193982
references.citation: Vet Parasitol. 2006 Jan 30;135(2):105-12
references.pmid: 16159697
references.citation: Vet Parasitol. 2003 Feb 13;111(2-3):153-9
references.pmid: 12531291
references.citation: Infect Genet Evol. 2006 Mar;6(2):113-22
references.pmid: 16503512
references.citation: Exp Parasitol. 2010 Jan;124(1):80-9
references.pmid: 19358845
references.citation: Vet Parasitol. 2011 May 11;177(3-4):366-70
references.pmid: 21242035
references.citation: Vet Parasitol. 2008 Aug 1;155(1-2):15-23
references.pmid: 18565677
references.citation: Vet Parasitol. 2011 Sep 27;181(2-4):321-4
references.pmid: 21612871
references.citation: J Med Microbiol. 2006 Jun;55(Pt 6):703-707
references.pmid: 16687587
references.citation: Appl Environ Microbiol. 1999 Apr;65(4):1578-83
references.pmid: 10103253
references.citation: Parasitology. 2007 Mar;134(Pt 3):339-50
references.pmid: 17076923
references.citation: J Dairy Sci. 1998 Nov;81(11):3036-41
references.pmid: 9839243
references.citation: Epidemiol Infect. 2007 Nov;135(8):1307-15
references.pmid: 17224087
references.citation: Vet Parasitol. 2008 Oct 1;156(3-4):191-8
references.pmid: 18583057
references.citation: J Vet Med B Infect Dis Vet Public Health. 2003 Nov;50(9):451-7
references.pmid: 14633200
references.citation: Vet Parasitol. 2009 Nov 12;165(3-4):187-99
references.pmid: 19660869
references.citation: J Clin Microbiol. 2011 Jan;49(1):34-41
references.pmid: 20980577
references.citation: J Clin Microbiol. 1985 Oct;22(4):587-9
references.pmid: 2416771
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: Vet Pathol. 2006 Mar;43(2):202-7
references.pmid: 16537941
references.citation: J Dairy Sci. 1995 May;78(5):1068-72
references.pmid: 7622718
references.citation: J Eukaryot Microbiol. 2006;53 Suppl 1:S43-8
references.pmid: 17169064
references.citation: J Clin Microbiol. 2005 Jun;43(6):2805-9
references.pmid: 15956401