title: Cardamonin suppresses the proliferation of colon cancer cells by promoting β-catenin degradation.
abstract: Aberrant accumulation of intracellular β-catenin and subsequent activation of β-catenin response transcription (CRT) in intestinal epithelial cells is a frequent early event during the development of colon cancer. Here we show that cardamonin, a chalcone isolated from Aplinia katsumadai Hayata, inhibited CRT in SW480 colon cancer cells that carry inactivating mutation in the adenomatous polyposis coli (APC) gene. Cardamonin also down-regulated intracellular β-catenin levels in SW480 cells without affecting its mRNA levels. Interestingly, pharmacological inhibition of the proteasome prevented the cardamonin-induced down-regulation of β-catenin. In addition, cardamonin suppressed the expression of cyclin D1 and c-myc, which are known β-catenin/T cell factor (TCF)-dependent genes. Moreover, cardamonin inhibited the growth of various colon cancer cells and induced G2/M cell cycle arrest in SW480 colon cancer cells. These findings indicate that cardamonin is a potential chemotherapeutic agent against colon cancer.
authors.affiliation: Department of Advanced Fermentation Fusion Science & Technology, Kookmin University, Seoul, Republic of Korea.
authors.forename: Seoyoung
authors.initials: S
authors.lastname: Park
authors.forename: Jungsug
authors.initials: J
authors.lastname: Gwak
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Han
authors.forename: Sangtaek
authors.initials: S
authors.lastname: Oh
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D047188:Chalcones; D051176:beta Catenin; C436747:cardamonin