title: Biomimetic antigenic nanoparticles elicit controlled protective immune response to influenza.
abstract: Here we present a biomimetic strategy toward nanoparticle design for controlled immune response through encapsulation of conserved internal influenza proteins on the interior of virus-like particles (VLPs) to direct CD8+ cytotoxic T cell protection. Programmed encapsulation and sequestration of the conserved nucleoprotein (NP) from influenza on the interior of a VLP, derived from the bacteriophage P22, results in a vaccine that provides multistrain protection against 100 times lethal doses of influenza in an NP specific CD8+ T cell-dependent manner. VLP assembly and encapsulation of the immunogenic NP cargo protein is the result of a genetically programmed self-assembly making this strategy amendable to the quick production of vaccines to rapidly emerging pathogens. Addition of adjuvants or targeting molecules were not required for eliciting the protective response.
authors.affiliation: Department of Chemistry and Biochemistry, Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana 59717, United States.
authors.forename: Dustin P
authors.initials: DP
authors.lastname: Patterson
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Rynda-Apple
authors.forename: Ann L
authors.initials: AL
authors.lastname: Harmsen
authors.forename: Allen G
authors.initials: AG
authors.lastname: Harmsen
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000008:administration & dosage / Q000138:chemical synthesis; D000949:Histocompatibility Antigens Class II / Q000008:administration & dosage* / Q000737:chemistry; D053118:Influenza A Virus, H1N1 Subtype; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control*; D016896:Treatment Outcome; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II; D058425:Vaccines, Virus-Like Particle
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