title: Among-population variation in microbial community structure in the floral nectar of the bee-pollinated forest herb Pulmonaria officinalis L.
abstract: BACKGROUND
  Microbial communities in floral nectar have been shown to be characterized by low levels of species diversity, yet little is known about among-plant population variation in microbial community composition.
  
  
  RESULTS
  We investigated the microbial community structure (yeasts and bacteria) in floral nectar of ten fragmented populations of the bee-pollinated forest herb Pulmonaria officinalis. We also explored possible relationships between plant population size and microbial diversity in nectar, and related microbial community composition to the distance separating plant populations. Culturable bacteria and yeasts occurring in the floral nectar of a total of 100 plant individuals were isolated and identified by partially sequencing the 16S rRNA gene and D1/D2 domains of the 26S rRNA gene, respectively. A total of 9 and 11 yeast and 28 and 39 bacterial OTUs was found, taking into account a 3% (OTU0.03) and 1% sequence dissimilarity cut-off (OTU0.01). OTU richness at the plant population level (i.e. the number of OTUs per population) was low for yeasts (mean: 1.7, range: 0-4 OTUs0.01/0.03 per population), whereas on average 6.9 (range: 2-13) OTUs0.03 and 7.9 (range 2-16) OTUs0.01 per population were found for bacteria. Both for yeasts and bacteria, OTU richness was not significantly related to plant population size. Similarity in community composition among populations was low (average Jaccard index: 0.14), and did not decline with increasing distance between populations.
  
  
  CONCLUSIONS
  We found low similarity in microbial community structure among populations, suggesting that the assembly of nectar microbiota is to a large extent context-dependent. Although the precise factors that affect variation in microbial community structure in floral nectar require further study, our results indicate that both local and regional processes may contribute to among-population variation in microbial community structure in nectar.
authors.affiliation: Division of Plant Ecology and Systematics, Biology Department, KU Leuven, Heverlee, Belgium. hans.jacquemyn@bio.kuleuven.be
authors.forename: Hans
authors.initials: H
authors.lastname: Jacquemyn
authors.forename: Marijke
authors.initials: M
authors.lastname: Lenaerts
authors.forename: Rein
authors.initials: R
authors.lastname: Brys
authors.forename: Kris
authors.initials: K
authors.lastname: Willems
authors.forename: Olivier
authors.initials: O
authors.lastname: Honnay
authors.forename: Bart
authors.initials: B
authors.lastname: Lievens
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001516:Bees / Q000502:physiology; D001530:Belgium; D044822:Biodiversity; D064307:Microbiota; D008969:Molecular Sequence Data; D010802:Phylogeny; D057048:Plant Nectar; D054817:Pollination; D029685:Pulmonaria / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014197:Trees; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D057048:Plant Nectar; D012336:RNA, Ribosomal, 16S
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