title: The effect of laser-treated titanium surface on human gingival fibroblast behavior.
abstract: Surface modification, as a means of enhancing soft tissue integration in titanium would have significant advantages including less marginal bone resorption, predictable esthetic outcome, improved soft tissue stability, and seal against bacterial leakage. The aim of this study was to evaluate the effects of laser-roughened titanium surfaces on human gingival fibroblast (HGF) viability, proliferation, and adhesion. Titanium discs were ablated with impulse laser in four different patterns. Polished and sand-blasted titanium discs were used as control groups. Specimen surface properties were determined using optical profilometry and scanning electron microscopy. HGF behavior on modified surfaces was analyzed using cell adhesion, viability, proliferation, and ELISA assays. Results suggested that modified Ti surfaces did not affect the viability of HGFs and improved adhesion was measured in laser treatment groups after 24 h. However, proliferation study showed that the adsorbance of fibroblast cells after 72 h cultured on polished titanium was higher and comparable with that of control cells. As for focal adhesion kinase (FAK), cells grown on laser modified surfaces had higher expression of FAK as compared with polished titanium. In conclusion, tested laser-treated surfaces seem to favor HGF adhesion. There were no significant differences between different laser treatment groups.
authors.affiliation: Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: D
authors.initials: D
authors.lastname: Baltriukienė
authors.forename: V
authors.initials: V
authors.lastname: Sabaliauskas
authors.forename: E
authors.initials: E
authors.lastname: Balčiūnas
authors.forename: A
authors.initials: A
authors.lastname: Melninkaitis
authors.forename: E
authors.initials: E
authors.lastname: Liutkevičius
authors.forename: V
authors.initials: V
authors.lastname: Bukelskienė
authors.forename: V
authors.initials: V
authors.lastname: Rutkūnas
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D015921:Dental Implants; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007834:Lasers; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D014025:Titanium; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: FAK; cell adhesion; cell proliferation; human gingival fibroblasts; titanium alloy