title: Distinctive microbial community structure in highly stratified deep-sea brine water columns.
abstract: Atlantis II and Discovery are two hydrothermal and hypersaline deep-sea pools in the Red Sea rift that are characterized by strong thermohalo-stratification and temperatures steadily peaking near the bottom. We conducted comprehensive vertical profiling of the microbial populations in both pools and highlighted the influential environmental factors. Pyrosequencing of the 16S rRNA genes revealed shifts in community structures vis-à-vis depth. High diversity and low abundance were features of the deepest convective layers despite the low cell density. Surprisingly, the brine interfaces had significantly higher cell counts than the overlying deep-sea water, yet they were lowest in diversity. Vertical stratification of the bacterial populations was apparent as we moved from the Alphaproteobacteria-dominated deep sea to the Planctomycetaceae- or Deferribacteres-dominated interfaces to the Gammaproteobacteria-dominated brine layers. Archaeal marine group I was dominant in the deep-sea water and interfaces, while several euryarchaeotic groups increased in the brine. Across sites, microbial phylotypes and abundances varied substantially in the brine interface of Discovery compared with Atlantis II, despite the near-identical populations in the overlying deep-sea waters. The lowest convective layers harbored interestingly similar microbial communities, even though temperature and heavy metal concentrations were very different. Multivariate analysis indicated that temperature and salinity were the major influences shaping the communities. The harsh conditions and the low-abundance phylotypes could explain the observed correlation in the brine pools.
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
authors.forename: S
authors.initials: S
authors.lastname: Bougouffa
authors.forename: J K
authors.initials: JK
authors.lastname: Yang
authors.forename: O O
authors.initials: OO
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Batang
authors.forename: A
authors.initials: A
authors.lastname: Al-Suwailem
authors.forename: P Y
authors.initials: PY
authors.lastname: Qian
mesh_terms: D001105:Archaea / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D044822:Biodiversity; D002452:Cell Count; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D060086:Hydrothermal Vents / Q000382:microbiology*; D019156:Indian Ocean; D054892:Metagenome / Q000235:genetics*; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S
references.citation: Trends Microbiol. 2010 Sep;18(9):413-22
references.pmid: 20663673
references.citation: Nat Methods. 2010 Sep;7(9):668-9
references.pmid: 20805793
references.citation: Nucleic Acids Res. 2007;35(21):7188-96
references.pmid: 17947321
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):12115-20
references.pmid: 16880384
references.citation: PLoS One. 2009 Oct 09;4(10):e7401
references.pmid: 19816594
references.citation: Bioinformatics. 2010 Jan 15;26(2):266-7
references.pmid: 19914921
references.citation: Nature. 2009 May 14;459(7244):193-9
references.pmid: 19444205
references.citation: ISME J. 2011 Mar;5(3):507-18
references.pmid: 20668490
references.citation: Mol Biotechnol. 2012 Jan;50(1):39-48
references.pmid: 21656086
references.citation: Environ Microbiol. 2007 Oct;9(10):2603-21
references.pmid: 17803783
references.citation: Appl Environ Microbiol. 2004 Feb;70(2):1190-8
references.pmid: 14766605
references.citation: Science. 2009 Oct 23;326(5952):578-82
references.pmid: 19900896
references.citation: Microbiol Rev. 1994 Mar;58(1):27-38
references.pmid: 8177169
references.citation: Nature. 1995 Jul 6;376(6535):57-8
references.pmid: 7541115
references.citation: Bioinformatics. 2004 Jun 12;20(9):1453-4
references.pmid: 14871861
references.citation: ISME J. 2010 Apr;4(4):520-30
references.pmid: 20033068
references.citation: Environ Microbiol. 2012 Apr;14(4):909-23
references.pmid: 22176665
references.citation: PLoS One. 2011;6(12):e29149
references.pmid: 22216192
references.citation: Arch Microbiol. 1999 Oct;172(4):213-8
references.pmid: 10525737
references.citation: Appl Environ Microbiol. 2009 Dec;75(23):7537-41
references.pmid: 19801464
references.citation: Nat Rev Microbiol. 2008 Apr;6(4):320-6
references.pmid: 18340342
references.citation: Genome Res. 2011 Mar;21(3):494-504
references.pmid: 21212162
references.citation: Environ Microbiol. 2012 Mar;14(3):714-29
references.pmid: 22050634
references.citation: Biochem Soc Trans. 2011 Dec;39(6):1859-63
references.pmid: 22103540
references.citation: Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):330-5
references.pmid: 23263870
references.citation: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references.pmid: 17586664
references.citation: Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22427-32
references.pmid: 20018741
references.citation: Bioinformatics. 2004 Nov 22;20(17):3246-8
references.pmid: 15180930
references.citation: Nature. 2001 May 3;411(6833):66-9
references.pmid: 11333977
references.citation: Microb Ecol. 2012 Feb;63(2):324-33
references.pmid: 21887519
references.citation: Nature. 2002 Dec 19-26;420(6917):806-10
references.pmid: 12490947
references.citation: Biochim Biophys Acta. 2002 Mar 25;1595(1-2):367-81
references.pmid: 11983409
references.citation: FEMS Microbiol Rev. 2009 Sep;33(5):855-69
references.pmid: 19453522
references.citation: Environ Microbiol. 2012 Jan;14(1):268-81
references.pmid: 22040283
references.citation: Trends Microbiol. 2001 Feb;9(2):79-86
references.pmid: 11173247
references.citation: Science. 1966 Dec 23;154(3756):1544-8
references.pmid: 17807292
references.citation: Chembiochem. 2010 Sep 24;11(14):2010-7
references.pmid: 20726028
references.citation: Appl Environ Microbiol. 2001 Jul;67(7):3077-85
references.pmid: 11425725
references.citation: Nature. 2005 Sep 22;437(7058):543-6
references.pmid: 16177789
references.citation: Nature. 1973 Dec 21-28;246(5434):525-7
references.pmid: 4586891
references.citation: J Bacteriol. 1962 Dec;84:1228-36
references.pmid: 14003850
references.citation: Nature. 2009 Oct 15;461(7266):976-9
references.pmid: 19794413
references.citation: ISME J. 2012 May;6(5):1032-45
references.pmid: 22134644
references.citation: Biochim Biophys Acta. 2012 Mar;1824(3):511-9
references.pmid: 22266402
references.citation: Bioinformatics. 2011 Apr 15;27(8):1159-61
references.pmid: 21349862
references.citation: Nature. 2001 Jan 25;409(6819):507-10
references.pmid: 11206545
references.citation: Environ Microbiol. 2010 Jul;12(7):1989-2006
references.pmid: 20345944
references.citation: BMC Genomics. 2006 Aug 23;7:216
references.pmid: 16928277
references.citation: Microb Ecol. 2010 Nov;60(4):771-83
references.pmid: 20740282
references.citation: PLoS Genet. 2009 Feb;5(2):e1000362
references.pmid: 19197347
references.citation: ISME J. 2011 Oct;5(10):1652-9
references.pmid: 21525946
references.citation: PLoS One. 2012;7(8):e42872
references.pmid: 22916172
references.citation: PLoS One. 2011;6(8):e23973
references.pmid: 21886845
references.citation: Ann Rev Mar Sci. 2011;3:197-225
references.pmid: 21329204
references.citation: Nat Methods. 2010 May;7(5):335-6
references.pmid: 20383131
references.citation: Appl Environ Microbiol. 2012 Mar;78(5):1361-9
references.pmid: 22194288
references.citation: Lett Appl Microbiol. 2012 Mar;54(3):209-16
references.pmid: 22191639
references.citation: Int J Syst Evol Microbiol. 2003 Mar;53(Pt 2):533-538
references.pmid: 12710623
references.citation: Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
references.pmid: 15034147
references.citation: Mol Biol Evol. 2009 Jul;26(7):1641-50
references.pmid: 19377059
references.citation: Environ Microbiol. 2007 Aug;9(8):2079-89
references.pmid: 17635551
references.citation: FEMS Microbiol Ecol. 2007 Jul;61(1):16-25
references.pmid: 17428303
references.citation: ISME J. 2011 Apr;5(4):650-64
references.pmid: 21085196