title: Microplates as a microreactor platform for microalgae research.
abstract: High-throughput platforms for microalgae screening are not yet commercially available. In this study, the feasibility of 96-well microplates was analyzed for microalgae research. Equivalence among wells, as culture microreactors, was investigated in controlled high CO2 conditions. Specific growth rates of two microalgae species, Scenedesmus sp. UTEX1589 and an environmental isolate, were significantly higher in border wells than in internal positions. Furthermore, growth rate gradients analyzed as contours throughout the platform were observed for Scenedesmus sp. However, the output variable exhibited high precision associated with a low coefficient of variation (CV), between 6.8 and 7.8%. In a demonstrative experiment to determine the effect of culture media dilution on six microalgae species, treatments were randomized in the central subset of a microplate. Results were consistent and statistically sound (CV 9.4-12.9%), and showed that microalgae species could grow with no detrimental effect in 50% (v/v) dilution of the culture medium. Provided border wells exclusion and a randomized design, 96-well microplates are a practical and statistical robust platform for microalgae research.
authors.affiliation: Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey, Campus Monterrey, Eugenio Garza Sada 2501, 64849, Monterrey, NL, México. adrianap@itesm.mx
authors.forename: Adriana
authors.initials: A
authors.lastname: Pacheco
authors.forename: Ileana
authors.initials: I
authors.lastname: Hernández-Mireles
authors.forename: Cecilia
authors.initials: C
authors.lastname: García-Martínez
authors.forename: Mario M
authors.initials: MM
authors.lastname: Alvarez
mesh_terms: D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D005240:Feasibility Studies; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods; D007700:Kinetics; D058086:Microalgae / Q000254:growth & development / Q000502:physiology*; D015203:Reproducibility of Results; D044444:Scenedesmus / Q000254:growth & development / Q000502:physiology
chemical_list: D002245:Carbon Dioxide