title: Impact of Lycium Barbarum Polysaccharide and Danshensu on vascular endothelial growth factor in the process of retinal neovascularization of rabbit.
abstract: OBJECTIVE
  To discuss the impact of Lycium Barbarum Polysaccharide (LBP) and Danshensu purified from Traditional Chinese Medicine (TCM) on vascular endothelial growth factor (VEGF) of rabbits with retinal neovascularization.
  
  
  METHODS
  Forty rabbits were divided into normal control group, model control group, LBP group and Danshensu group. Animals in the normal control group were fed in the normal oxygen environment. Animals in the other three groups were put into the environment with 70% oxygen for 5 days in order to build the model of oxygen-induced vascular proliferation retinopathy. And then different TCM extract was injected into the abdominal cavities of these annimals. After 7 days, the VEGF content of in the serum of rabbit was measured by double antibody sandwich method.
  
  
  RESULTS
  DATA ANALYSIS INDICATED THAT VEGF CONTENT WAS AS FOLLOWS: Danshensu group was lower than model control group (12.92±3.84ng/L vs 19.32±4.15ng/L, P<0.05); LBP group and normal control group were lower than model control group (12.92±3.84ng/L, 9.26±1.61ng/L vs 19.32±4.15ng/L, P<0.01); total blood viscosity, plasma viscosity, cholesterol content, fibrinogen content and triacylglycerol content after peritoneal injection of LBP and Danshensu were obviously lower than before injection.
  
  
  CONCLUSIONS
  TCM extract-LBP and Danshensu can prominently reduce the content of VEGF in the process of vascular proliferative retinopathy of rabbit; can prevent the occurrence of retinal microvascular disease by improving partial oxygen-deficient environment or affecting all kinds of new growth factor.
authors.affiliation: Department of Ophthalmology, No.153 Hospital of Liberation Army, Zhengzhou 450042, Henan Province, China.
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Tian
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Bai-Ke
authors.initials: BK
authors.lastname: Zhang
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.forename: Shi-Jin
authors.initials: SJ
authors.lastname: Zhang
keywords: Danshensu; Lycium Barbarum Polysaccharide; rabbit; retinal neovascularization; vascular endothelial growth factor
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