title: Highly sensitive detection of thrombin using SERS-based magnetic aptasensors.
abstract: This paper reports a method of highly sensitive detection of thrombin using a surface-enhanced Raman scattering (SERS)-based magnetic aptasensor. Magnetic beads and gold nanoparticles (Au NPs) were used as supporting substrates and sensing probes, respectively. For this purpose, 15-mer thrombin-binding aptamers (TBA15) were immobilized onto the surface of magnetic beads, and then thrombin antigens and 29-mer thrombin-binding aptamer (TBA29)-conjugated Au NPs were sequentially added for the formation of sandwich aptamer complexes. Quantitative analysis was performed by monitoring the intensity variation of a characteristic SERS signal of Raman reporter molecules. Because all of the reactions occur in solution, this SERS-based immunoassay technique can solve the diffusion-limited kinetic problems on a solid substrate. The limit of detection (LOD) of thrombin, determined by the SERS-based aptasensor, was estimated to be 0.27pM. The proposed method is expected to be a good clinical tool for the diagnosis of a thrombotic disease.
authors.affiliation: Department of Bionano Engineering, Hanyang University, Sa-1-dong 1271, Ansan 426-791, Republic of Korea.
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Yoon
authors.forename: Namhyun
authors.initials: N
authors.lastname: Choi
authors.forename: Juhui
authors.initials: J
authors.lastname: Ko
authors.forename: Kihyung
authors.initials: K
authors.lastname: Kim
authors.forename: Sangyeop
authors.initials: S
authors.lastname: Lee
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
mesh_terms: D000941:Antigens / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D006801:Humans; D018189:Immunomagnetic Separation; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013917:Thrombin / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000941:Antigens; D052157:Aptamers, Nucleotide; C081676:thrombin aptamer; D006046:Gold; D013917:Thrombin