title: Short-term sleep deprivation reinstates memory retrieval in mice: the role of corticosterone secretion.
abstract: While the effects of sleep deprivation (SD) on the acquisition and consolidation phases of memory have been extensively characterized, its effects on memory retrieval remain overlooked. SD alone is a stressor, and stress-activated glucocorticoids promote bimodal effects on memory. Because we have recently demonstrated that 72h SD impairs memory retrieval in the plus-maze discriminative avoidance task (PM-DAT) in mice, this study investigated whether shorter SD periods would facilitate retrieval. In Experiment I, the temporal forgetting curve of the PM-DAT was determined and an interval between training/testing in which retrieval was no longer present was used in all subsequent experiments. In Experiments II and III, retrieval performance and corticosterone concentration, respectively, were quantified in mice that were sleep deprived for 12 or 24h before testing. In Experiments IV and V, the effects of the corticosterone synthesis inhibitor metyrapone were evaluated on 12h SD-induced retrieval reinstatement and corticosterone concentration enhancement, respectively. Experiment VI determined whether pre-test acute administration of exogenous corticosterone would mimic the facilitatory effects of 12h SD on retrieval. Thirty days after training, mice presented poor performance of the task; however, SD for 12h (but not for 24) before testing reinstated memory retrieval. This facilitatory effect was accompanied by increased corticosterone concentration, abolished by metyrapone, and mimicked by pre-test acute corticosterone administration. Collectively, short-term SD can facilitate memory retrieval by enhancing corticosterone secretion. This facilitatory effect is abolished by longer periods of SD.
authors.affiliation: Departamento de Farmacologia, Universidade Federal de São Paulo, Rua Botucatu 862, Ed. Leal Prado, 1(o) andar, 04023062 São Paulo, SP, Brazil.
authors.forename: André L
authors.initials: AL
authors.lastname: Takatsu-Coleman
authors.forename: Karina A
authors.initials: KA
authors.lastname: Zanin
authors.forename: Camilla L
authors.initials: CL
authors.lastname: Patti
authors.forename: Adriano
authors.initials: A
authors.lastname: Zager
authors.forename: Leonardo B
authors.initials: LB
authors.lastname: Lopes-Silva
authors.forename: Beatriz M
authors.initials: BM
authors.lastname: Longo
authors.forename: Sergio
authors.initials: S
authors.lastname: Tufik
authors.forename: Monica L
authors.initials: ML
authors.lastname: Andersen
authors.forename: Roberto
authors.initials: R
authors.lastname: Frussa-Filho
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D003345:Corticosterone / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects / Q000502:physiology; D011939:Mental Recall / Q000187:drug effects / Q000502:physiology*; D008797:Metyrapone / Q000494:pharmacology; D051379:Mice; D012892:Sleep Deprivation / Q000097:blood / Q000503:physiopathology / Q000523:psychology*; D013315:Stress, Psychological / Q000503:physiopathology / Q000523:psychology; D013997:Time Factors
chemical_list: D003345:Corticosterone; D008797:Metyrapone
keywords: Corticosterone; Memory; Metyrapone; Sleep deprivation; Stress