title: Sponge-derived Kocuria and Micrococcus spp. as sources of the new thiazolyl peptide antibiotic kocurin.
abstract: Forty four marine actinomycetes of the family Microccocaceae isolated from sponges collected primarily in Florida Keys (USA) were selected from our strain collection to be studied as new sources for the production of bioactive natural products. A 16S rRNA gene based phylogenetic analysis showed that the strains are members of the genera Kocuria and Micrococcus. To assess their biosynthetic potential, the strains were PCR screened for the presence of secondary metabolite genes encoding nonribosomal synthetase (NRPS) and polyketide synthases (PKS). A small extract collection of 528 crude extracts generated from nutritional microfermentation arrays was tested for the production of bioactive secondary metabolites against clinically relevant strains (Bacillus subtilis, methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumannii and Candida albicans). Three independent isolates were shown to produce a new anti-MRSA bioactive compound that was identified as kocurin, a new member of the thiazolyl peptide family of antibiotics emphasizing the role of this family as a prolific resource for novel drugs.
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Armilla, Granada, Spain. sara.palomo@medinaandalucia.es
authors.forename: Sara
authors.initials: S
authors.lastname: Palomo
authors.forename: Ignacio
authors.initials: I
authors.lastname: González
authors.forename: Mercedes
authors.initials: M
authors.lastname: de la Cruz
authors.forename: Jesús
authors.initials: J
authors.lastname: Martín
authors.forename: José Rubén
authors.initials: JR
authors.lastname: Tormo
authors.forename: Matthew
authors.initials: M
authors.lastname: Anderson
authors.forename: Russell T
authors.initials: RT
authors.lastname: Hill
authors.forename: Francisca
authors.initials: F
authors.lastname: Vicente
authors.forename: Fernando
authors.initials: F
authors.lastname: Reyes
authors.forename: Olga
authors.initials: O
authors.lastname: Genilloud
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D015373:Bacterial Typing Techniques; D002176:Candida albicans / Q000187:drug effects; D005285:Fermentation; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008835:Micrococcaceae / Q000302:isolation & purification / Q000378:metabolism*; D008837:Micrococcus / Q000302:isolation & purification / Q000378:metabolism; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011161:Porifera / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; C583478:kocurin
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