title: α-Cyclodextrin decreases cholera toxin binding to GM1-gangliosides.
abstract: Cholera toxin (CT), the principal virulence factor secreted by Vibrio cholerae, is an A-B5 type exotoxin that binds to host cell GM1-gangliosides and is responsible for cholera diarrhoea. We tested the hypothesis that the cyclic hexasaccharide α-cyclodextrin (α-CD), but not the cyclic heptasaccharides methyl-β-cyclodextrin (MD-β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) inhibit binding of CT to GM1-gangliosides. We report that α-CD decreases CT binding to GM1-ganglioside-coated microtitre plate wells and on the surface of fixed HeLa cells in a concentration-dependent manner, suggesting that this may be a promising lead for the development of compounds with therapeutic properties.
authors.affiliation: Department of Biological Sciences, University of Texas Brownsville, 80 Fort Brown, Brownsville, TX 78520, USA.|Department of Biomedical Sciences, University of Texas Brownsville, 80 Fort Brown, Brownsville, TX 78520, USA.
authors.forename: Boris
authors.initials: B
authors.lastname: Ermolinsky
authors.affiliation: Department of Biological Sciences, University of Texas Brownsville, 80 Fort Brown, Brownsville, TX 78520, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Peredelchuk
authors.affiliation: Department of Biological Sciences, University of Texas Brownsville, 80 Fort Brown, Brownsville, TX 78520, USA.|Department of Biomedical Sciences, University of Texas Brownsville, 80 Fort Brown, Brownsville, TX 78520, USA.
authors.forename: Daniele
authors.initials: D
authors.lastname: Provenzano
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002772:Cholera Toxin / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005732:Gangliosides / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D047391:alpha-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D005732:Gangliosides; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002772:Cholera Toxin; C032613:alpha-cyclodextrin
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