title: Influence of pH, metal chelator, free radical scavenger and interfacial characteristics on the oxidative stability of β-carotene in conjugated whey protein-pectin stabilised emulsion.
abstract: The influence of whey protein isolate (WPI)-beet pectin conjugates formed by dry-heating on the oxidative stability of β-carotene in O/W emulsions was studied. It was mainly focused on the influence of pH, metal chelator, free radical scavenger and interfacial characteristics on the degradation of β-carotene in the emulsion stabilised by conjugate. The conjugate increased the oxidative stability of β-carotene in the emulsion as compared to their unconjugated mixture at pH 7.0. The desferoxamine retarded β-carotene degradation at pH 4.0 more effectively than pH 7.0 and more effectively in the emulsion with the conjugate than the unconjugated mixture (p<0.05). The addition of 200 mg/kg α-tocopherol significantly improved the stability of β-carotene in the conjugate stabilised emulsion. The emulsions were washed to remove conjugate not absorbed to the emulsion droplet interface, indicating that unabsorbed emulsifiers could protect β-carotene. It suggested that WPI-pectin conjugate could be used to protect bioactive lipids in emulsions.
authors.affiliation: College of Food Science & Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing, China.
authors.forename: Duoxia
authors.initials: D
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Gao
authors.forename: D Julian
authors.initials: DJ
authors.lastname: McClements
authors.forename: Eric A
authors.initials: EA
authors.lastname: Decker
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers / Q000737:chemistry; D004355:Drug Stability; D045703:Emulsifying Agents / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry*; D010084:Oxidation-Reduction; D010368:Pectins / Q000737:chemistry*; D000067816:Whey Proteins; D019207:beta Carotene / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D004337:Drug Carriers; D045703:Emulsifying Agents; D004655:Emulsions; D016166:Free Radical Scavengers; D008670:Metals; D008894:Milk Proteins; D000067816:Whey Proteins; D019207:beta Carotene; D010368:Pectins