title: The effects of RFRP-3, the mammalian ortholog of GnIH, on the female pig reproductive axis in vitro.
abstract: RFamide-related peptide-3 (RFRP-3) has been proposed as a key inhibitory regulator of mammalian reproduction. To further determine the potential mechanisms and sites of action of RFRP-3, we systematically investigated the direct effect of RFRP-3 on the female pig reproductive axis in vitro. Initially, we confirmed that G protein-coupled receptor 147 (GPR147) was distributed in isolated hypothalamic, anterior pituitary and ovarian granulosa cells, suggesting that RFRP-3 could act on these cells in vitro. Subsequently, the direct effects of RFRP-3 on hormone and steroid secretion, the synthesis of subunit genes and the expression of proteins related to proliferation in the hypothalamus, pituitary and ovary were evaluated. Our results demonstrate that different doses of RFRP-3 inhibited the release and synthesis of gonadotrophin releasing hormone, gonadotrophin and steroid hormones and impacted the relative gene expression of KISS1 and GnRHR and the protein expression of cyclin B1, PCNA and ERK 1/2.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xun
authors.initials: X
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Su
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.forename: Lucheng
authors.initials: L
authors.lastname: Zheng
authors.forename: Ruipeng
authors.initials: R
authors.lastname: Lei
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Wang
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Lei
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Jin
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.forename: Yuanlong
authors.initials: Y
authors.lastname: Hou
authors.forename: Tingting
authors.initials: T
authors.lastname: Guo
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D004958:Estradiol / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D006062:Gonadotropins / Q000235:genetics / Q000378:metabolism; D006107:Granulosa Cells / Q000378:metabolism; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D007031:Hypothalamus / Q000166:cytology; D009479:Neuropeptides / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000166:cytology; D011374:Progesterone / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000378:metabolism; D055703:Reproductive Physiological Phenomena; D034421:Sus scrofa / Q000502:physiology*
chemical_list: D006062:Gonadotropins; D009479:Neuropeptides; C416987:RFamide peptide; D018013:Receptors, Neuropeptide; C083929:neuropeptide FF receptor; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol