title: Kinesin KIFC1 actively transports bare double-stranded DNA.
abstract: During the past years, exogenous DNA molecules have been used in gene and molecular therapy. At present, it is not known how these DNA molecules reach the cell nucleus. We used an in cell single-molecule approach to observe the motion of exogenous short DNA molecules in the cytoplasm of eukaryotic cells. Our observations suggest an active transport of the DNA along the cytoskeleton filaments. We used an in vitro motility assay, in which the motion of single-DNA molecules along cytoskeleton filaments in cell extracts is monitored; we demonstrate that microtubule-associated motors are involved in this transport. Precipitation of DNA-bound proteins and mass spectrometry analyses reveal the preferential binding of the kinesin KIFC1 on DNA. Cell extract depletion of kinesin KIFC1 significantly decreases DNA motion, confirming the active implication of this molecular motor in the intracellular DNA transport.
authors.affiliation: Physico-Chimie-Curie/UMR168 Institut Curie, Centre National de la Recherche Scientiﬁque, Université Pierre et Marie Curie, 75231 Paris, France.
authors.forename: Francesca
authors.initials: F
authors.lastname: Farina
authors.forename: Paolo
authors.initials: P
authors.lastname: Pierobon
authors.forename: Cédric
authors.initials: C
authors.lastname: Delevoye
authors.forename: Jordan
authors.initials: J
authors.lastname: Monnet
authors.forename: Florent
authors.initials: F
authors.lastname: Dingli
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.forename: Maria
authors.initials: M
authors.lastname: Quanz
authors.forename: Marie
authors.initials: M
authors.lastname: Dutreix
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cappello
mesh_terms: D001693:Biological Transport, Active; D003599:Cytoskeleton / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000032:analysis / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D008870:Microtubules / Q000378:metabolism
chemical_list: C486566:KIFC1 protein, human; D004247:DNA; D004398:Dyneins; D016547:Kinesins
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