title: Embossing of micropatterned ceramics and their cellular response.
abstract: The aim of this work is to investigate the use of microtopographies in providing physical cues to modulate the cellular response of human mesenchymal stem cells on ceramics. Two microgrooved patterns (100 μm/50 μm, 10 μm/10 μm groove/pitch) were transcribed reversely onto alumina green ceramic tapes via an embossing technique followed by sintering. Characterization of the micropatterned alumina surfaces and their cellular response was carried out. Spread and polygonal cell morphologies were observed on the wider groove (50 μm/100 μm) surface. Cells seeded onto the narrow groove (10 μm/10 μm) surface aligned themselves alongside the grooves, resulting in more elongated cell morphology. More osteoid matrix nodules shown by osteopontin and osteocalcin biomarkers were detected on the larger grooved surfaces after cell culture of 21 days, indicating a greater level of osteogenicity. This study has shown that micropatterned wider groove (50 μm) topographies are more suitable surfaces for improving osseointegration of ceramic implants.
authors.affiliation: School of Oral and Dental Sciences, University of Bristol, Bristol, BS1 2LY, United Kingdom.
authors.forename: Danish
authors.initials: D
authors.lastname: Nadeem
authors.forename: Terje
authors.initials: T
authors.lastname: Sjostrom
authors.forename: Andrew
authors.initials: A
authors.lastname: Wilkinson
authors.forename: Carol-Anne
authors.initials: CA
authors.lastname: Smith
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.forename: Matt J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Bo
authors.initials: B
authors.lastname: Su
mesh_terms: D000537:Aluminum Oxide / Q000494:pharmacology; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000378:metabolism; D002516:Ceramics / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000378:metabolism; D015676:Osteonectin / Q000378:metabolism; D016133:Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015676:Osteonectin; D015675:Osteocalcin; D052006:Bone Morphogenetic Protein Receptors, Type II; D000537:Aluminum Oxide
keywords: ceramics; embossing; micropatterns; osseointegration; stem cells