title: A retrospective study of the prevalence and classification of intestinal neoplasia in zebrafish (Danio rerio).
abstract: For over a decade, spontaneous intestinal neoplasia has been observed in zebrafish (Danio rerio) submitted to the ZIRC (Zebrafish International Resource Center) diagnostic service. In addition, zebrafish displayed preneoplastic intestinal changes including hyperplasia, dysplasia, and enteritis. A total of 195 zebrafish, representing 2% of the total fish submitted to the service, were diagnosed with these lesions. Neoplastic changes were classified either as adenocarcinoma or small cell carcinoma, with a few exceptions (carcinoma not otherwise specified, tubular adenoma, and tubulovillous adenoma). Tumor prevalence appeared similarly distributed between sexes and generally occurred in zebrafish greater than 1 year of age, although neoplastic changes were observed in fish 6 months of age. Eleven lines displayed these preneoplastic and neoplastic changes, including wild-types and mutants. Affected zebrafish originated from 18 facilities, but the majority of fish were from a single zebrafish research facility (hereafter referred to as the primary facility) that has submitted numerous samples to the ZIRC diagnostic service. Zebrafish from the primary facility submitted as normal sentinel fish demonstrate that these lesions are most often subclinical. Fish fed the diet from the primary facility and held at another location did not develop intestinal lesions, indicating that diet is not the etiologic agent.
authors.affiliation: Department of Microbiology, Oregon State University, Corvallis, OR 97331-3804, USA.
authors.forename: Colleen E
authors.initials: CE
authors.lastname: Paquette
authors.forename: Michael L
authors.initials: ML
authors.lastname: Kent
authors.forename: Cari
authors.initials: C
authors.lastname: Buchner
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
authors.forename: Karen
authors.initials: K
authors.lastname: Guillemin
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Mason
authors.forename: Tracy S
authors.initials: TS
authors.lastname: Peterson
mesh_terms: D000230:Adenocarcinoma / Q000145:classification / Q000453:epidemiology* / Q000209:etiology; D000367:Age Factors; D000821:Animal Feed / Q000009:adverse effects / Q000032:analysis; D000818:Animals; D058345:Asymptomatic Infections / Q000453:epidemiology; D018288:Carcinoma, Small Cell / Q000145:classification / Q000453:epidemiology* / Q000209:etiology; D004032:Diet / Q000009:adverse effects / Q000662:veterinary; D005260:Female; D005393:Fish Diseases / Q000145:classification / Q000453:epidemiology* / Q000209:etiology; D007414:Intestinal Neoplasms / Q000145:classification / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary*; D008297:Male; D015995:Prevalence; D012189:Retrospective Studies; D012737:Sex Factors; D015027:Zebrafish
references.citation: Mod Pathol. 2003 Sep;16(9):913-9
references.pmid: 13679455
references.citation: J Clin Oncol. 2004 Jul 1;22(13):2730-9
references.pmid: 15226341
references.citation: Cancer Res. 1998 Oct 1;58(19):4255-9
references.pmid: 9766647
references.citation: Am J Anat. 1974 Dec;141(4):503-19
references.pmid: 4216261
references.citation: Comp Med. 2002 Aug;52(4):354-8
references.pmid: 12211280
references.citation: Prog Clin Pathol. 1984;9:121-33
references.pmid: 6364219
references.citation: Toxicol Pathol. 2003 Jan-Feb;31 Suppl:62-87
references.pmid: 12597434
references.citation: Trends Biotechnol. 2006 Jan;24(1):15-21
references.pmid: 16309768
references.citation: Biol Bull. 2002 Oct;203(2):265-6
references.pmid: 12414614
references.citation: J Comp Pathol. 2013 Feb;148(2-3):220-4
references.pmid: 22819012
references.citation: Vet Pathol. 2013 May;50(3):468-82
references.pmid: 23203679
references.citation: ILAR J. 2012;53(2):135-43
references.pmid: 23382345
references.citation: Mar Biotechnol (NY). 2001 Jun;3(Supplement 1):S88-99
references.pmid: 14961304
references.citation: Annu Rev Pathol. 2011;6:71-93
references.pmid: 21261518
references.citation: Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):407-12
references.pmid: 15630097
references.citation: Endocrinol Metab Clin North Am. 2011 Mar;40(1):111-34, viii
references.pmid: 21349414
references.citation: Toxicol Pathol. 2011 Aug;39(5):759-75
references.pmid: 21636695
references.citation: J Virol. 1992 Jan;66(1):596-9
references.pmid: 1727503
references.citation: Vet Parasitol. 2010 Feb 26;168(1-2):71-7
references.pmid: 19963322
references.citation: Vet Pathol. 2010 May;47(3):553-9
references.pmid: 20351360
references.citation: Cancer Res. 2009 May 15;69(10):4355-62
references.pmid: 19417130
references.citation: Zebrafish. 2009 Dec;6(4):361-76
references.pmid: 19929219
references.citation: Mech Dev. 2005 Feb;122(2):157-73
references.pmid: 15652704
references.citation: Best Pract Res Clin Endocrinol Metab. 2007 Mar;21(1):15-31
references.pmid: 17382263
references.citation: Virchows Arch. 2006 Oct;449(4):402-9
references.pmid: 16955307
references.citation: ILAR J. 2012;53(2):114-25
references.pmid: 23382343
references.citation: Oncogene. 2008 Jul 31;27(33):4509-20
references.pmid: 18372910
references.citation: J Virol. 2004 Jan;78(2):899-911
references.pmid: 14694121
references.citation: Dev Biol. 2003 Mar 1;255(1):12-29
references.pmid: 12618131
references.citation: J Natl Cancer Inst. 1972 Feb;48(2):325-46
references.pmid: 4652379
references.citation: Toxicol Pathol. 2000 Sep-Oct;28(5):705-15
references.pmid: 11026607
references.citation: Acta Pathol Microbiol Scand Suppl. 1961;Suppl 152:1-151
references.pmid: 14462807
references.citation: Int Rev Cell Mol Biol. 2008;271:1-34
references.pmid: 19081540
references.citation: EMBO Rep. 2006 Apr;7(4):444-9
references.pmid: 16439994
references.citation: Am J Pathol. 1979 Jul;96(1):5-20
references.pmid: 37740
references.citation: Cancer Res. 1992 Dec 1;52(23):6496-500
references.pmid: 1384964
references.citation: Dis Aquat Organ. 2011 May 24;95(1):73-9
references.pmid: 21797038