title: Ras guanyl nucleotide releasing protein 2 affects cell viability and cell-matrix adhesion in ECV304 endothelial cells.
abstract: Ras guanyl nucleotide releasing proteins (RasGRPs) are guanine nucleotide exchange factors that activate Ras and Rap. We recently reported that xrasgrp2, which is a homolog of the human rasgrp2, plays a role in vasculogenesis and/or angiogenesis during early development of Xenopus embryos. However, the function of RasGRP2 in human vascular endothelium remains unknown. Therefore we aimed to analyze the function of human RasGRP2 in vascular endothelial cells. RasGRP2 overexpression did not increase Ras activation. However, it slightly increased Ras expression and increased proliferation in ECV304 cells. Furthermore, RasGRP2 overexpression increased Rap1 activation and cell-matrix adhesion in ECV304 cells. These data demonstrate that RasGRP2 increases cell viability and cell-matrix adhesion through increased Ras expression and Rap1 activation, respectively, in endothelial cells.
authors.affiliation: Laboratory of Biochemistry, Hiroshima International University, Hiroshima, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Takino
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nagamine
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Hori
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D021982:Cell-Matrix Junctions / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D015398:Signal Transduction; D020827:rap1 GTP-Binding Proteins / Q000378:metabolism*; D020663:ras Guanine Nucleotide Exchange Factors / Q000378:metabolism*
chemical_list: D020662:Guanine Nucleotide Exchange Factors; C415483:RASGRP2 protein, human; D020663:ras Guanine Nucleotide Exchange Factors; D020827:rap1 GTP-Binding Proteins
keywords: Rap1; Ras; RasGRP2; cell proliferation; cell–matrix adhesion; endothelial cells
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