title: Environmentally safe treatment of black liquor with Comamonas sp. B-9 under high-alkaline conditions.
abstract: The strain Comamonas sp. B-9 was isolated from steeping fluid of erosive bamboo slips derived from Kingdom Wu during the Three-Kingdoms Dynasty of ancient China (A.D. 220-280). It could be used to treat black liquor (BL) with high-alkaline pH and with an initial chemical oxygen demand (COD) of 18,000-25,000 mg L(-1) , without the addition of other carbon and nitrogen sources. The results revealed that Comamonas sp. B-9 was capable of reducing the COD, color, and lignin content of BL by up to 56.8, 35.3, and 43.5%, respectively. High levels of laccase, manganese peroxidase, cellulase, and xylanase enzymatic activities were also observed, and these enzymes could play an important role in the biotreatment of BL. Further, GC-MS analysis showed that most of the compounds detected in BL after biotreatment with Comamonas sp. B-9 were diminished, while 4-methyl benzaldehyde, 3,4,5-trihydroxybenzoic acid ethyl ester, and 4-hydroxy-3,5-dimethoxy benzaldehyde were produced as metabolites. The presented results indicate that Comamonas sp. B-9 has potential application for the treatment of wastewaters from pulp and paper processing with high COD load under high-alkaline conditions.
authors.affiliation: Institute of Environmental Science and Engineering, School of Metallurgical Science and Engineering, Central South University, Changsha, P. R., China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Liyuan
authors.initials: L
authors.lastname: Chai
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yang
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001673:Biodegradation, Environmental; D020585:Comamonas / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008031:Lignin / Q000378:metabolism; D006109:Poaceae / Q000382:microbiology*; D062065:Wastewater
chemical_list: D007220:Industrial Waste; D008031:Lignin; D062065:Wastewater
keywords: Biotreatment; Black liquor; Comamonas sp. B-9; High-alkaline conditions; Lignocellulolytic enzymes