title: Differential requirement of Oryza sativa RAR1 in immune receptor-mediated resistance of rice to Magnaporthe oryzae.
abstract: The required for Mla12 resistance (RAR1) protein is essential for the plant immune response. In rice, a model monocot species, the function of Oryza sativa RAR1 (OsRAR1) has been little explored. In our current study, we characterized the response of a rice osrar1 T-DNA insertion mutant to infection by Magnaporthe oryzae, the causal agent of rice blast disease. osrar1 mutants displayed reduced resistance compared with wild type rice when inoculated with the normally virulent M. oryzae isolate PO6-6, indicating that OsRAR1 is required for an immune response to this pathogen. We also investigated the function of OsRAR1 in the resistance mechanism mediated by the immune receptor genes Pib and Pi5 that encode nucleotide binding-leucine rich repeat (NB-LRR) proteins. We inoculated progeny from Pib/osrar1 and Pi5/osrar1 heterozygous plants with the avirulent M. oryzae isolates, race 007 and PO6-6, respectively. We found that only Pib-mediated resistance was compromised by the osrar1 mutation and that the introduction of the OsRAR1 cDNA into Pib/osrar1 rescued Pib-mediated resistance. These results indicate that OsRAR1 is required for Pib-mediated resistance but not Pi5-mediated resistance to M. oryzae.
authors.affiliation: Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea.
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Song
authors.forename: Chi-Yeol
authors.initials: CY
authors.lastname: Kim
authors.forename: Muho
authors.initials: M
authors.lastname: Han
authors.forename: Hak-Seung
authors.initials: HS
authors.lastname: Ryu
authors.forename: Sang-Kyu
authors.initials: SK
authors.lastname: Lee
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Zuhua
authors.initials: Z
authors.lastname: He
authors.forename: Young-Su
authors.initials: YS
authors.lastname: Seo
authors.forename: Patrick
authors.initials: P
authors.lastname: Canal
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
authors.forename: Jong-Seong
authors.initials: JS
authors.lastname: Jeon
mesh_terms: D020082:Magnaporthe / Q000235:genetics / Q000276:immunology*; D012275:Oryza / Q000235:genetics / Q000276:immunology*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D010940:Plant Proteins
references.citation: Mol Plant Microbe Interact. 2005 May;18(5):397-404
references.pmid: 15915638
references.citation: Plant Cell. 2011 Jan;23(1):4-15
references.pmid: 21278123
references.citation: PLoS Genet. 2011 Apr;7(4):e1002020
references.pmid: 21533176
references.citation: Mol Plant Microbe Interact. 2008 Mar;21(3):294-303
references.pmid: 18257679
references.citation: Science. 2009 Nov 6;326(5954):850-3
references.pmid: 19892983
references.citation: Genetics. 2009 Apr;181(4):1627-38
references.pmid: 19153255
references.citation: Plant J. 2011 May;66(3):467-79
references.pmid: 21251109
references.citation: Plant J. 2006 Jun;46(5):794-804
references.pmid: 16709195
references.citation: Biochem Biophys Res Commun. 2006 Jun 30;345(2):646-51
references.pmid: 16690022
references.citation: PLoS Pathog. 2006 Jan;2(1):e2
references.pmid: 16424920
references.citation: EMBO J. 2003 Nov 3;22(21):5679-89
references.pmid: 14592967
references.citation: Plant J. 2006 Jan;45(1):123-32
references.pmid: 16367959
references.citation: Trends Plant Sci. 2003 Jun;8(6):252-8
references.pmid: 12818658
references.citation: Science. 2002 Mar 15;295(5562):2073-6
references.pmid: 11847307
references.citation: Planta. 2006 Aug;224(3):598-611
references.pmid: 16552590
references.citation: Mol Plant Pathol. 2010 May;11(3):419-27
references.pmid: 20447289
references.citation: Plant Cell. 2005 Apr;17(4):1268-78
references.pmid: 15749757
references.citation: Science. 2009 May 8;324(5928):742-4
references.pmid: 19423812
references.citation: Annu Rev Plant Biol. 2009;60:139-64
references.pmid: 19014346
references.citation: Plant Cell. 2002 May;14(5):979-92
references.pmid: 12034891
references.citation: Plant Physiol. 2007 May;144(1):312-23
references.pmid: 17351050
references.citation: Plant Physiol. 2005 Aug;138(4):2165-73
references.pmid: 16024691
references.citation: Plant J. 1999 Mar;17(5):535-45
references.pmid: 10205906
references.citation: Science. 2002 Mar 15;295(5562):2077-80
references.pmid: 11847308
references.citation: Annu Rev Plant Biol. 2003;54:23-61
references.pmid: 14502984
references.citation: J Biol Chem. 2004 Jan 16;279(3):2101-8
references.pmid: 14583611
references.citation: Trends Biochem Sci. 2010 Apr;35(4):199-207
references.pmid: 20096590
references.citation: EMBO J. 2003 Nov 3;22(21):5690-9
references.pmid: 14592968
references.citation: Plant Cell. 2002 May;14(5):1005-15
references.pmid: 12034893
references.citation: Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10865-9
references.pmid: 12119413
references.citation: Theor Appl Genet. 2004 Sep;109(5):978-85
references.pmid: 15141293
references.citation: Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18395-400
references.pmid: 17082309
references.citation: Plant Cell. 2004 Dec;16(12):3480-95
references.pmid: 15548741
references.citation: Plant J. 2004 Dec;40(5):647-59
references.pmid: 15546349
references.citation: Plant Cell Physiol. 2005 Jan;46(1):14-22
references.pmid: 15659434
references.citation: Plant Physiol. 2011 Sep;157(1):109-19
references.pmid: 21771914
references.citation: Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11777-82
references.pmid: 14504384
references.citation: Plant J. 2005 Jul;43(2):284-98
references.pmid: 15998314
references.citation: Science. 2005 Aug 5;309(5736):929-32
references.pmid: 15976272
references.citation: Cell. 1999 Nov 12;99(4):355-66
references.pmid: 10571178
references.citation: Plant J. 1999 Jul;19(1):55-64
references.pmid: 10417726
references.citation: Plant Mol Biol. 2005 Sep;59(1):111-23
references.pmid: 16217606
references.citation: Curr Opin Plant Biol. 2007 Aug;10(4):335-41
references.pmid: 17652011
references.citation: Phytopathology. 2002 Aug;92(8):870-6
references.pmid: 18942966
references.citation: Nature. 2006 Nov 16;444(7117):323-9
references.pmid: 17108957
references.citation: Nature. 2004 Apr 15;428(6984):764-7
references.pmid: 15085136