title: Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout.
abstract: Teleost fish express highly diverse naive TCRβ (TRB) repertoires and mount strong public and private clonal responses upon infection with pathogens. Fish T cells express typical markers such as CD8, CD4-1 and CD4-2, CD3, CD28 and CTLA4. Fish CD8(+) T cells have been shown to be responsible for antigen-specific cell-mediated cytotoxicity in in vitro systems using histo-compatible effector and target cells. We compare here the complexity of TRB repertoires between FACS sorted CD8(+) and CD8(-) T cells from spleen and pronephros of rainbow trout. In contrast to human, while the TRB repertoire is highly diverse and polyclonal in CD8(+) T cells of naïve fish, it appeared very different in CD8(-) lymphocytes with irregular CDR3 length distributions suggesting a dominance of activated clones already in naïve fish or the presence of non conventional T cells. After infection with a systemic virus, CD8(+) T cells mount a typical response with significant skewing of CDR3 length profiles. The infection also induces significant modifications of the TRB repertoire expressed by the CD8(-) fraction, but for a different set of V/J combinations. In this fraction, the antiviral response results in an increase of the peak diversity of spectratypes. This unusual observation reflects the presence of a number of T cell expansions that rise the relative importance of minor peaks of the highly skewed distributions observed in unchallenged animals. These results suggest that the diversity of TRB expressed by CD8(+) and CD8(-) αβ T cells may be subjected to different regulatory patterns in fish and in mammals.
authors.affiliation: Institut National de la Recherche Agronomique, Virologie et Immunologie Moléculaires, Jouy-en-Josas, France.
authors.forename: Rosario
authors.initials: R
authors.lastname: Castro
authors.forename: Fumio
authors.initials: F
authors.lastname: Takizawa
authors.forename: Wahiba
authors.initials: W
authors.lastname: Chaara
authors.forename: Aurélie
authors.initials: A
authors.lastname: Lunazzi
authors.forename: Thi Huong
authors.initials: TH
authors.lastname: Dang
authors.forename: Bernd
authors.initials: B
authors.lastname: Koellner
authors.forename: Edwige
authors.initials: E
authors.lastname: Quillet
authors.forename: Adrien
authors.initials: A
authors.lastname: Six
authors.forename: Uwe
authors.initials: U
authors.lastname: Fischer
authors.forename: Pierre
authors.initials: P
authors.lastname: Boudinot
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta
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