title: Limited perturbation of a DPPC bilayer by fluorescent lipid probes: a molecular dynamics study.
abstract: The properties of lipid bilayer nanometer-scale domains could be crucial for understanding cell membranes. Fluorescent probes are often used to study bilayers, yet their effects on host lipids are not well understood. We used molecular dynamics simulations to investigate perturbations in a fluid DPPC bilayer upon incorporation of three indocarbocyanine probes: DiI-C18:0, DiI-C18:2, or DiI-C12:0. We find a 10-12% decrease in chain order for DPPC in the solvation shell nearest the probe but smaller effects in subsequent shells, indicating that the probes significantly alter only their local environment. We also observe order perturbations of lipids directly across from the probe in the opposite leaflet. Additionally, the DPPC headgroup phosphorus-to-nitrogen vector of lipids nearest the probe exhibits preferential orientation pointing away from the DiI. We show that, while DiI probes perturb their local environment, they do not strongly influence the average properties of "nanoscopic" domains containing a few hundred lipids.
authors.affiliation: Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, United States.
authors.forename: David G
authors.initials: DG
authors.lastname: Ackerman
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Heberle
authors.forename: Gerald W
authors.initials: GW
authors.lastname: Feigenson
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D012997:Solvents / Q000737:chemistry; D055672:Static Electricity
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D012997:Solvents; D015060:1,2-Dipalmitoylphosphatidylcholine; D002244:Carbon
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