title: Antioxidant status and oxidative stress markers in pancreatic cancer and chronic pancreatitis.
abstract: OBJECTIVE
  Oxidative stress has been implicated in the pathogenesis of chronic pancreatitis (CP) and pancreatic cancer (PC). The study aim was to assess the oxidative stress markers and antioxidant defense system in patients with CP and those with PC.
  
  
  METHODS
  Activities of superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), glutathione reductase (GR), arylesterase (PON1-A) and lactonase (PON1-L) activities of paraoxonase 1 (PON1) and concentrations of reduced glutathione, conjugated dienes in low-density lipoprotein (CD/LDL) and oxidized LDL (ox-LDL/LDL) were assessed in 50 PC and 50 CP patients and 50 age and sex-matched controls.
  
  
  RESULTS
  Comparison of PC and CP groups to controls found the following changes: glutathione peroxidase 1 (GPX1) (-20.2%, -25.5%; P < 0.001), glutathione reductase (GR) (-9.5%, -11.9%; P < 0.05), SOD1 (+22.9%; P < 0.01), CAT (-10.6%; P < 0.05), PON1-A (-34.3%, -16.0%; P < 0.001), PON1-L (-44.2%; -17.0%; P < 0.01), conjugated dienes in LDL (CD/LDL) (+20%, +33.3%; P < 0.05) and ox-LDL/LDL (+42.2%, +14.4%; P < 0.05). The patients with PC had changed activities and levels of SOD1 (+24.2%), CAT (-10.4); P < 0.01), PON1-A (-21.7%), PON1-L (-32.9%), and ox-LDL/LDL (+24.3%); (all P < 0.01) compared with the patients with CP.
  
  
  CONCLUSIONS
  Reduced antioxidant defense system capacity and increased markers of oxidative stress were found in PC and CP. PON1-L and CAT activities, along with ox-LDL/LDL levels, were the independent factors differentiating the patients with PC from the patients with CP.
authors.affiliation: Fourth Department of Internal Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital Prague, Czech Republic. Jana.Kodydkova@seznam.cz
authors.forename: Jana
authors.initials: J
authors.lastname: Kodydkova
authors.forename: Lucie
authors.initials: L
authors.lastname: Vavrova
authors.forename: Barbora
authors.initials: B
authors.lastname: Stankova
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Macasek
authors.forename: Tomas
authors.initials: T
authors.lastname: Krechler
authors.forename: Ales
authors.initials: A
authors.lastname: Zak
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000201:enzymology; D050500:Pancreatitis, Chronic / Q000097:blood* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000097:blood; D000072105:Superoxide Dismutase-1
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014408:Biomarkers, Tumor; D008077:Lipoproteins, LDL; C000606290:SOD1 protein, human; C099252:oxidized low density lipoprotein; D002374:Catalase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human