title: Adipose stromal cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity and reactive oxygen species.
abstract: A recent in vivo study suggested that the delivery of adipose stromal cells (ASCs) protected rat brains from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. However, the molecular mechanism that underlies this neuroprotection remains unknown. It was suggested that ASCs-induced neuroprotection possibly resulting from released factors from ASCs. In this study, we investigated whether and how cell-free conditioned media collected from ASCs (ASC-CM) protect neurons against neurotoxicity induced by 6-OHDA in cultured rat rostral mesencephalic neurons (RMN) and cerebellar granule neurons (CGN). We now report that ASC-CM protects both RMN and CGN against 6-OHDA neurotoxicity. Exposure of CGN to 6-OHDA resulted in a significant increases in neuronal ROS and cell death. As expected, pretreatments with ASC-CM dramatically block both 6-OHDA-induced ROS and neurotoxicity. Additionally, ASC-CM also directly attenuated H2O2-induced neuronal death. Our results suggest that ASC-CM could block 6-OHDA-induced neuronal death by inhibiting both 6-OHDA-induced ROS generation and ROS-induced neurotoxicity in neurons. Both antioxidative and neuroprotective effects of ASC-CM may be beneficial in the therapy for Parkinson's disease and other neurodegenerative diseases.
authors.affiliation: Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Huiying
authors.initials: H
authors.lastname: Gu
authors.forename: Jimmy
authors.initials: J
authors.lastname: Wang
authors.forename: Nicole
authors.initials: N
authors.lastname: Du
authors.forename: Jiangning
authors.initials: J
authors.lastname: Tan
authors.forename: Brian
authors.initials: B
authors.lastname: Johnstone
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Du
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D000818:Animals; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D009474:Neurons / Q000187:drug effects / Q000473:pathology* / Q000502:physiology*; D016627:Oxidopamine / Q000633:toxicity*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D017382:Reactive Oxygen Species; D016627:Oxidopamine