title: Detection of HPV by PCR-A Novel Step in the Prevention of Cancer Cervix.
abstract: OBJECTIVE
  (1) To compare the efficacy of Pap smear and HPV PCR for detection of CIN; and (2) To study the distribution of HPV genotypes.
  
  
  METHODS
  One hundred women presenting at the female Outpatient Department with unhealthy cervices were subjected to a detailed history, clinical examination, Pap smear, HPV DNA PCR test, and colposcopic-directed biopsy (where indicated).
  
  
  RESULTS
  This study has shown that there is a strong association of HPV infection with higher grades of CIN (100 % in patients with CIN 2, CIN 3, and CIS). The detection of CIN by HPV PCR was more accurate than by Pap smear. The most prevalent HPV genotype found in our study was HPV 16.
  
  
  CONCLUSIONS
  The advent of HPV testing has opened the doors for more accurate cervical cancer surveillance strategies than Pap smear. Early detection and treatment of CIN will considerably reduce the incidence of cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Medical Sciences, Banaras Hindu University, 91 A, Opposite Baba Kinaram Temple, Ravindrapuri, Varanasi, 221005 India.
authors.forename: Manjari
authors.initials: M
authors.lastname: Matah
authors.forename: Sweta
authors.initials: S
authors.lastname: Sareen
keywords: Cancer cervix; Cervical intraepithelial neoplasia; Human Papillomavirus; Polymerase chain reaction
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