title: Changes of free radicals and digestive enzymes in saliva in cases with deficiency in spleen-yin syndrome.
abstract: OBJECTIVE
  To explore the nature of deficiency in spleen-yin syndrome, which could provide scientific theoretical support and practical guidance for clinical Traditional Chinese Medicine (TCM) syndrome differentiation based on biology, and had a strong clinical significance.
  
  
  METHODS
  Serum Cu and Zn were detected by atomic absorption spectrophotometer, serum vitamin E by high performance liquid chromatography, serum vitamin C by 2,4-Dinitrophenylhydrazine Colorimetry, total superoxide dismutase (SOD) and Cu and Zn-SOD by the xanthine oxidase method, and malondialdehyde (MDA) by the 2-thiobarbituric acid method (TBA). Total antioxidant capacity was detected by a colorimetry kit. Amylase Activity was detected by an automatic biochemical analyzer. Lysozyme was detected by lysozyme detection plate, the diameter of bacteriolysis circle was measured and the corresponding content of lysozyme was obtained from a table of standard curve values.
  
  
  RESULTS
  No significant difference in total SOD and Cu, Zn-SOD was found between deficiency in spleen-yin group and normal group. However, such factors in deficiency in kidney-yin group were significantly lower than the other groups (P < 0.05). The MDA content in both deficiency in spleen-yin group and deficiency in kidney-yin group were significantly higher than that of normal group (P < 0.05), while the total antioxidant capacity was significantly lower than normal group (P < 0.05). The vitamin E content in deficiency in kidney-yin group was significantly lower than that in the other two groups (P < 0.05). No significant difference in the contents of vitamin C, Cu and Zn were observed in these groups. The Zn/Cu level in deficiency in kidney-yin group and the vitamin E level in deficiency in spleen-yin group decreased, but with no significant difference. Amylase activity in unit time in cases with deficiency in spleen-yin was lower than and had significant differences with that in normal cases, and higher than that in cases with deficiency in kidney-yin. The sectional velocity of saliva and the ratio of lysozyme in normal case group were significantly higher than other two groups, while deficiency in the spleen-yin group was significantly higher than the deficiency in kidney-yin group.
  
  
  CONCLUSIONS
  All the results indicated that the objective pathological mechanism between the deficiency in spleen-yin and deficiency in kidney-yin was different.
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, Jingsu Province, China.
authors.forename: Muxin
authors.initials: M
authors.lastname: Wei
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Wu
authors.forename: Dezheng
authors.initials: D
authors.lastname: Chen
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Gu
keywords: amylase; deficiency in kidney-yin; deficiency in spleen-yin; digestive enzymes in saliva; free radicals; salivary flow rate; salivary lysozyme
references.citation: Growth Factors. 2009 Oct;27(5):300-8
references.pmid: 19629819
references.citation: Oral Dis. 2005 Jul;11(4):230-5
references.pmid: 15984954
references.citation: Hepatol Int. 2009 Dec;3(4):526-36
references.pmid: 19941170
references.citation: Nutr Res Rev. 2009 Jun;22(1):49-67
references.pmid: 19555518
references.citation: Clin Endocrinol (Oxf). 2009 Mar;70(3):469-74
references.pmid: 18727709
references.citation: J Nutr Sci Vitaminol (Tokyo). 2009 Feb;55(1):1-8
references.pmid: 19352057
references.citation: Free Radic Biol Med. 2009 Dec 15;47(12):1673-706
references.pmid: 19751821
references.citation: Leuk Lymphoma. 2009 May;50(5):809-15
references.pmid: 19452319
references.citation: Cell Death Differ. 2009 Jul;16(7):1040-52
references.pmid: 19407826
references.citation: Braz J Med Biol Res. 2009 Jun;42(6):537-44
references.pmid: 19448903
references.citation: Arch Dermatol Res. 2009 Oct;301(10):731-7
references.pmid: 19488773
references.citation: Metabolism. 2009 Nov;58(11):1657-62
references.pmid: 19604518
references.citation: Recent Pat Cardiovasc Drug Discov. 2009 Jun;4(2):76-87
references.pmid: 19519550
references.citation: Free Radic Biol Med. 2009 Sep 15;47(6):786-93
references.pmid: 19539750
references.citation: Food Chem Toxicol. 2009 Aug;47(8):1914-20
references.pmid: 19445998
references.citation: J Dent Res. 2008 Aug;87(8):772-6
references.pmid: 18650551
references.citation: J Biomed Mater Res A. 2006 Sep 15;78(4):755-61
references.pmid: 16739107
references.citation: J Am Diet Assoc. 2003 Aug;103(8):1029-38
references.pmid: 12891154
references.citation: Redox Rep. 2009;14(2):69-74
references.pmid: 19389274