title: Chitosan(PEO)/silica hybrid nanofibers as a potential biomaterial for bone regeneration.
abstract: New hybrid nanofibers prepared with chitosan (CTS), containing a total amount of polyethylene oxide (PEO) down to 3.6wt.%, and silica precursors were produced by electrospinning. The solution of modified sol-gel particles contained tetraethoxysilane (TEOS) and the organosilane 3-glycidyloxypropyltriethoxysilane (GPTEOS). This is rending stable solution toward gelation and contributing in covalent bonding with chitosan. The fibers encompass advantages of biocompatible polymer template silicate components to form self-assembled core-shell structure of the polymer CTS/PEO encapsulated by the silica. Potential applicability of this hybrid material to bone tissue engineering was studied examining its cellular compatibility and bioactivity. The nanofiber matrices were proved cytocompatible when seeded with bone-forming 7F2-cells, promoting attachment and proliferation over 7 days. These found to enhance a fast apatite formation by incorporation of Ca(2+) ions and subsequent immersion in modified simulated body fluid (m-SBF). The tunable properties of these hybrid nanofibers can find applications as active biomaterials in bone repair and regeneration.
authors.affiliation: Institute of Textile Machinery and High Performance Material Technology (ITM), Technische Universität Dresden, Hohe Str. 6, 01069 Dresden, Germany. georg.toskas@gmx.de
authors.forename: Georgios
authors.initials: G
authors.lastname: Toskas
authors.forename: Chokri
authors.initials: C
authors.lastname: Cherif
authors.forename: Rolf-Dieter
authors.initials: RD
authors.lastname: Hund
authors.forename: Ezzeddine
authors.initials: E
authors.lastname: Laourine
authors.forename: Boris
authors.initials: B
authors.lastname: Mahltig
authors.forename: Amir
authors.initials: A
authors.lastname: Fahmi
authors.forename: Christiane
authors.initials: C
authors.lastname: Heinemann
authors.forename: Thomas
authors.initials: T
authors.lastname: Hanke
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D012821:Silanes; D048271:Chitosan; D017886:Durapatite