title: PodNet, a protein-protein interaction network of the podocyte.
abstract: Interactions between proteins crucially determine cellular structure and function. Differential analysis of the interactome may help elucidate molecular mechanisms during disease development; however, this analysis necessitates mapping of expression data on protein-protein interaction networks. These networks do not exist for the podocyte; therefore, we built PodNet, a literature-based mouse podocyte network in Cytoscape format. Using database protein-protein interactions, we expanded PodNet to XPodNet with enhanced connectivity. In order to test the performance of XPodNet in differential interactome analysis, we examined podocyte developmental differentiation and the effect of cell culture. Transcriptomes of podocytes in 10 different states were mapped on XPodNet and analyzed with the Cytoscape plugin ExprEssence, based on the law of mass action. Interactions between slit diaphragm proteins are most significantly upregulated during podocyte development and most significantly downregulated in culture. On the other hand, our analysis revealed that interactions lost during podocyte differentiation are not regained in culture, suggesting a loss rather than a reversal of differentiation for podocytes in culture. Thus, we have developed PodNet as a valuable tool for differential interactome analysis in podocytes, and we have identified established and unexplored regulated interactions in developing and cultured podocytes.
authors.affiliation: Department of Anatomy and Cell Biology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Warsow
authors.forename: Nicole
authors.initials: N
authors.lastname: Endlich
authors.forename: Eric
authors.initials: E
authors.lastname: Schordan
authors.forename: Sandra
authors.initials: S
authors.lastname: Schordan
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Chilukoti
authors.forename: Georg
authors.initials: G
authors.lastname: Homuth
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Moeller
authors.forename: Georg
authors.initials: G
authors.lastname: Fuellen
authors.forename: Karlhans
authors.initials: K
authors.lastname: Endlich
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D030562:Databases, Protein; D005786:Gene Expression Regulation; D051379:Mice; D050199:Podocytes / Q000378:metabolism*; D025341:Principal Component Analysis; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D011506:Proteins