title: Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9.
abstract: Eukaryotic genomes harbor transposable elements and other repetitive sequences that must be silenced. Small RNA interference pathways play a major role in their repression. Here, we reveal another mechanism for silencing these sequences in Drosophila. Depleting the linker histone H1 in vivo leads to strong activation of these elements. H1-mediated silencing occurs in combination with the heterochromatin-specific histone H3 lysine 9 methyltransferase Su(var)3-9. H1 physically interacts with Su(var)3-9 and recruits it to chromatin in vitro, which promotes H3 methylation. We propose that H1 plays a key role in silencing by tethering Su(var)3-9 to heterochromatin. The tethering function of H1 adds to its established role as a regulator of chromatin compaction and accessibility.
authors.affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Xingwu
authors.initials: X
authors.lastname: Lu
authors.forename: Sandeep N
authors.initials: SN
authors.lastname: Wontakal
authors.forename: Harsh
authors.initials: H
authors.lastname: Kavi
authors.forename: Byung Ju
authors.initials: BJ
authors.lastname: Kim
authors.forename: Paloma M
authors.initials: PM
authors.lastname: Guzzardo
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Emelyanov
authors.forename: Na
authors.initials: N
authors.lastname: Xu
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Hannon
authors.forename: Jiri
authors.initials: J
authors.lastname: Zavadil
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Fyodorov
authors.forename: Arthur I
authors.initials: AI
authors.lastname: Skoultchi
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D020868:Gene Silencing; D006570:Heterochromatin / Q000378:metabolism*; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics; D034622:RNA Interference; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D006570:Heterochromatin; D006657:Histones; D009124:Muscle Proteins; D012097:Repressor Proteins; C089148:Su(var)3-9 protein, Drosophila; D014157:Transcription Factors; C066817:nau protein, Drosophila
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