title: Mapping quantitative trait loci for tissue culture response in VCS3M-DH population of Brassica rapa.
abstract: Quantitative trait loci (QTL) controlling callus induction and plant regeneration were identified in the VCS3M-DH population of Brassica rapa. Quantitative trait loci (QTL) controlling callus induction and plant regeneration were identified in the VCS3M-DH population of Brassica rapa. The VCS3M-DH population showed wide and continuous variation in callus induction and shoot regeneration. Significant coefficient correlations were detected between these two parameters. Broad-sense heritability (h (2)) for the two traits was around 0.7, indicating genetic regulation of regeneration ability in this population. In the composite interval mapping analysis, two QTLs for callus induction ability, qCi2 and qCi7, were mapped on chromosome A02 and A07, explaining 28.6 % of phenotypic variation. For plant regeneration, four QTLs, qPr6-1 qPr6-2, qPr7, and qPr9 were identified on chromosome A06, A07, and A09, which in total explained 50.1 % of phenotypic variation. Furthermore, 15 putative candidate genes were found on the interval of the six QTLs, which were related to various plant hormones, MADS-box genes, and serine/threonine related genes. These results provide important information to identify genes related to tissue culture ability in B. rapa.
authors.affiliation: Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, 150 Suin-ro, Gwonseon-gu, Suwon, 441-707, Korea. sms1030@korea.kr
authors.forename: Mi-Suk
authors.initials: MS
authors.lastname: Seo
authors.forename: Mina
authors.initials: M
authors.lastname: Jin
authors.forename: Soo-Seong
authors.initials: SS
authors.lastname: Lee
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Mun
authors.forename: Beom-Seok
authors.initials: BS
authors.lastname: Park
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
authors.forename: Guusje
authors.initials: G
authors.lastname: Bonnema
authors.forename: Seong-Han
authors.initials: SH
authors.lastname: Sohn
mesh_terms: D000704:Analysis of Variance; D001937:Brassica / Q000235:genetics* / Q000502:physiology*; D002874:Chromosome Mapping; D056726:Genetic Association Studies; D006238:Haploidy; D040582:Inheritance Patterns / Q000235:genetics; D010641:Phenotype; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology; D040641:Quantitative Trait Loci / Q000235:genetics*; D012038:Regeneration / Q000502:physiology; D046509:Tissue Culture Techniques
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