title: Determination of cyclamate in urine by derivatized gas chromatography-mass spectrometry.
abstract: OBJECTIVE
  It is important in toxicological/drug screening work to rule out the possible interfering analytes, to eliminate the false positive or negative results. In this paper, we describe a simple, selective, and sensitive derivatized GC-MS method for the determination of cyclohexylsulfamic acid (cyclamate) in urine.
  
  
  METHODS
  Elite- 5MS capillary column was used for the separation of analytes and detection using GC-MS. The analysis was carried out in selected ion monitoring mode (SIM) in the range of 26 to 200 using m/z values of 57, 30, 55, 41, 44, 67, 82, 98, and 39.
  
  
  CONCLUSIONS
  The method is based on the conversion of cyclamate into nitroso derivative of cyclamate followed by its gas chromatography-mass spectrometry determination. The limit of detection, limit of quantitation, and linearity range of the proposed method were found to be 0.2 μg/ ml, 0.7 μg/ml, and 1-15 μg/ml, respectively. The recovery of the present method is in the range of 88-94%.
  
  
  CONCLUSIONS
  The proposed method can be applied for detection and quantification of cyclamate in urine.
authors.affiliation: Central Forensic Science Laboratory, Ministry of Home Affairs, Govt. of India, Ramanthapur, Hyderabad, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Idris
authors.forename: Deepak
authors.initials: D
authors.lastname: Middha
authors.forename: Shaik N
authors.initials: SN
authors.lastname: Rasool
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Shukla
authors.forename: Tulsidas R
authors.initials: TR
authors.lastname: Baggi
keywords: Cyclamate; GC-MS; screening; toxicology; urine
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