title: Aminosilane functionalizations of mesoporous oxidized silicon for oligonucleotide synthesis and detection.
abstract: Direct solid phase synthesis of peptides and oligonucleotides (ONs) requires high chemical stability of the support material. In this work, we have investigated the passivation ability of porous oxidized silicon multilayered structures by two aminosilane compounds, 3-aminopropyltriethoxysilane and 3-aminopropyldimethylethoxysilane (APDMES), for optical label-free ON biosensor fabrication. We have also studied by spectroscopic reflectometry the hybridization between a 13 bases ON, directly grown on the aminosilane modified porous oxidized silicon by in situ synthesis, and its complementary sequence. Even if the results show that both devices are stable to the chemicals (carbonate/methanol) used, the porous silica structure passivated by APDMES reveals higher functionalization degree due to less steric hindrance of pores.
authors.affiliation: National Council of Research, Institute for Microelectronics and Microsystems, Via P. Castellino 111, Naples, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: De Stefano
authors.forename: Giorgia
authors.initials: G
authors.lastname: Oliviero
authors.forename: Jussara
authors.initials: J
authors.lastname: Amato
authors.forename: Nicola
authors.initials: N
authors.lastname: Borbone
authors.forename: Gennaro
authors.initials: G
authors.lastname: Piccialli
authors.forename: Luciano
authors.initials: L
authors.lastname: Mayol
authors.forename: Ivo
authors.initials: I
authors.lastname: Rendina
authors.forename: Monica
authors.initials: M
authors.lastname: Terracciano
authors.forename: Ilaria
authors.initials: I
authors.lastname: Rea
mesh_terms: D015374:Biosensing Techniques; D009841:Oligonucleotides / Q000138:chemical synthesis*; D016062:Porosity; D011437:Propylamines / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques
chemical_list: C582385:(3-aminopropyl)dimethylethoxysilane; D009841:Oligonucleotides; D011437:Propylamines; D012821:Silanes; C477625:amino-propyl-triethoxysilane; D012825:Silicon
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