title: Oral administration of heparin or heparosan increases the Lactobacillus population in gut microbiota of rats.
abstract: Heparin and heparosan have been confirmed to be effective blockers in inhibiting adhesion of pathogens in vitro. However, their effects on gut microbiota in vivo remain unknown. Here we have studied the effects of oral administration of heparin or heparosan on gut microbiota in rats by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Results showed that the predominant bacterial communities in the feces of heparin- or heparosan-treated animals were different from those of the saline-treated animals, with increased Lactobacillus spp. and decreased Enterococcus sp. Different DGGE banding patterns were also observed for the subpopulations of Lactobacillus and Bacteroides groups. In conclusion, heparin or heparosan may be used as an effective gut microbiota modulator by increasing the subpopulation of Lactobacillus.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors.forename: Rongshuai
authors.initials: R
authors.lastname: Duan
authors.forename: Xiang'e
authors.initials: X
authors.lastname: Chen
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
authors.forename: Tianmin
authors.initials: T
authors.lastname: Zhang
authors.forename: Peixue
authors.initials: P
authors.lastname: Ling
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001439:Bacteroides / Q000235:genetics; D003013:Clostridium / Q000187:drug effects / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004187:Disaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D016983:Enterococcus / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005798:Genes, Bacterial; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology*; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics*; D008297:Male; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004187:Disaccharides; D012336:RNA, Ribosomal, 16S; C560363:heparosan; D006493:Heparin