title: Role of serotonin in zebrafish (Danio rerio) anxiety: relationship with serotonin levels and effect of buspirone, WAY 100635, SB 224289, fluoxetine and para-chlorophenylalanine (pCPA) in two behavioral models.
abstract: Serotonin (5-HT) is a neurotransmitter that is involved in many behavioral functions, including the organization of defense, and its putative pathological correlate, anxiety and stress disorders. Recently, behavioral tests for anxiety have been proposed in zebrafish. Exposure to the novel tank test or to the light/dark test increased extracellular fluid 5-HT content in the brain; anxiety-like behavior correlated positively with 5-HT content in the novel tank test, while the correlation was negative in the light/dark test. Acute treatment with a low dose of fluoxetine was anxiolytic in the geotaxis test and anxiogenic in the scototaxis test, while treatment with a higher dose produced a hyperlocomotor effect in both tasks. Buspirone and WAY 100635 were anxiolytic in both tests, while SB 224289 was anxiolytic in the geotaxis and slightly anxiogenic in the scototaxis test. Serotonin depletion with pCPA was anxiogenic in the geotaxis and anxiolytic in scototaxis. These results underline the differential sensitivity of these tasks to assess serotonergic agents; alternatively, serotonin might regulate zebrafish behavior differently in the novel tank test and in the light/dark test.
authors.affiliation: Laboratório de Neuroendocrinologia, Instituto de Ciências Biológicas, UFPa, Belém, PA, Brazil. caio@ufpa.br
authors.forename: Caio
authors.initials: C
authors.lastname: Maximino
authors.forename: Bruna
authors.initials: B
authors.lastname: Puty
authors.forename: Rancés
authors.initials: R
authors.lastname: Benzecry
authors.forename: Juliana
authors.initials: J
authors.lastname: Araújo
authors.forename: Monica Gomes
authors.initials: MG
authors.lastname: Lima
authors.forename: Evander
authors.initials: E
authors.lastname: de Jesus Oliveira Batista
authors.forename: Karen
authors.initials: K
authors.lastname: Renata de Matos Oliveira
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Crespo-Lopez
authors.forename: Anderson Manoel
authors.initials: AM
authors.lastname: Herculano
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D001007:Anxiety / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002065:Buspirone / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D045604:Extracellular Fluid / Q000187:drug effects / Q000378:metabolism; D010134:Fenclonine / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D005473:Fluoxetine / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D006948:Hyperkinesis / Q000139:chemically induced / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010879:Piperazines / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D010881:Piperidones / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D020033:Protein Isoforms / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D011725:Pyridines / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D012701:Serotonin / Q000737:chemistry / Q000378:metabolism*; D058825:Serotonin 5-HT1 Receptor Agonists / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use; D058829:Serotonin 5-HT1 Receptor Antagonists / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use; D017367:Selective Serotonin Reuptake Inhibitors / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use; D013141:Spiro Compounds / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D015027:Zebrafish
chemical_list: D014151:Anti-Anxiety Agents; D002065:Buspirone; D010134:Fenclonine; D005473:Fluoxetine; D009419:Nerve Tissue Proteins; D010879:Piperazines; D010881:Piperidones; D020033:Protein Isoforms; D011725:Pyridines; D012701:Serotonin; D058825:Serotonin 5-HT1 Receptor Agonists; D058829:Serotonin 5-HT1 Receptor Antagonists; D017367:Selective Serotonin Reuptake Inhibitors; D013141:Spiro Compounds; C108518:SB 22489G; C090413:N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide