title: Colorimetric and fluorometric assays based on conjugated polydiacetylene supramolecules for screening acetylcholinesterase and its inhibitors.
abstract: Polydiacetylene supramolecules (PDAs) are unique sensing materials. Upon environmental stimulation, blue PDAs can undergo a colorimetric transition from blue to red accompanied by fluorescence enhancement. In this paper, we report a new PDA system polymerized from a mixed liposome comprising 2-(2-(2-hydroxyethoxy)ethoxy)ethyl pentacosa-10,12-diynoate and pentacosa-10,12-diynoic acid at a 3:7 ratio. The PDA system provided new colorimetric and fluorometric assay methods for screening acetylcholinesterase and its inhibitors through three processes. First, myristoylcholine reacted with PDAs, which then underwent colorimetric and fluorometric transition. Second, acetylcholinesterase catalyzed the hydrolysis of myristoylcholine into tetradecanoic acid, which reduced the myristoylcholine concentration and led to faded color and fluorescence. Third and last, acetylcholinesterase inhibitors retarded the activity of acetylcholinesterase, thereby inducing the recovery of color and fluorescence.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing 210009, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Huilin
authors.initials: H
authors.lastname: Wang
authors.forename: Srinivasulu
authors.initials: S
authors.lastname: Kambam
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Chen
authors.forename: Juyoung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D005470:Fluorometry / Q000379:methods*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*
chemical_list: D002800:Cholinesterase Inhibitors; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D000110:Acetylcholinesterase