title: Human CD1c+ dendritic cells drive the differentiation of CD103+ CD8+ mucosal effector T cells via the cytokine TGF-β.
abstract: In comparison to murine dendritic cells (DCs), less is known about the function of human DCs in tissues. Here, we analyzed, by using lung tissues from humans and humanized mice, the role of human CD1c(+) and CD141(+) DCs in determining the type of CD8(+) T cell immunity generated to live-attenuated influenza virus (LAIV) vaccine. We found that both lung DC subsets acquired influenza antigens in vivo and expanded specific cytotoxic CD8(+) T cells in vitro. However, lung-tissue-resident CD1c(+) DCs, but not CD141(+) DCs, were able to drive CD103 expression on CD8(+) T cells and promoted CD8(+) T cell accumulation in lung epithelia in vitro and in vivo. CD1c(+) DCs induction of CD103 expression was dependent on membrane-bound cytokine TGF-β1. Thus, CD1c(+) and CD141(+) DCs generate CD8(+) T cells with different properties, and CD1c(+) DCs specialize in the regulation of mucosal CD8(+) T cells.
authors.affiliation: Baylor Institute for Immunology Research, Dallas, TX 75204, USA.
authors.forename: Chun I
authors.initials: CI
authors.lastname: Yu
authors.forename: Christian
authors.initials: C
authors.lastname: Becker
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Florentina
authors.initials: F
authors.lastname: Marches
authors.forename: Julie
authors.initials: J
authors.lastname: Helft
authors.forename: Marylene
authors.initials: M
authors.lastname: Leboeuf
authors.forename: Esperanza
authors.initials: E
authors.lastname: Anguiano
authors.forename: Stephane
authors.initials: S
authors.lastname: Pourpe
authors.forename: Kristina
authors.initials: K
authors.lastname: Goller
authors.forename: Virginia
authors.initials: V
authors.lastname: Pascual
authors.forename: Jacques
authors.initials: J
authors.lastname: Banchereau
authors.forename: Miriam
authors.initials: M
authors.lastname: Merad
authors.forename: Karolina
authors.initials: K
authors.lastname: Palucka
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000378:metabolism; D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D018928:Immunity, Mucosal; D007156:Immunologic Memory; D007252:Influenza Vaccines / Q000276:immunology; D039001:Integrin alpha Chains / Q000378:metabolism; D008168:Lung / Q000276:immunology* / Q000821:virology; D008213:Lymphocyte Activation; D051379:Mice; D016513:Mice, SCID; D046228:Microarray Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D000956:Antigens, Viral; C437944:CD1C protein, human; D006023:Glycoproteins; D007252:Influenza Vaccines; D039001:Integrin alpha Chains; D016212:Transforming Growth Factor beta; C091367:alpha E integrins
references.citation: Semin Immunol. 1995 Oct;7(5):335-42
references.pmid: 8580465
references.citation: Nature. 1994 Nov 10;372(6502):190-3
references.pmid: 7969453
references.citation: J Exp Med. 2010 Jun 7;207(6):1273-81
references.pmid: 20479115
references.citation: Immunity. 2008 Jun;28(6):870-80
references.pmid: 18513999
references.citation: Am J Respir Cell Mol Biol. 2005 Mar;32(3):177-84
references.pmid: 15576669
references.citation: Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1924-8
references.pmid: 1542691
references.citation: J Biol Chem. 1992 Aug 15;267(23):16607-12
references.pmid: 1379600
references.citation: J Immunol. 2011 Jun 1;186(11):6207-17
references.pmid: 21515786
references.citation: Eur J Immunol. 2011 Sep;41(9):2585-95
references.pmid: 21660938
references.citation: Nat Immunol. 2009 May;10(5):524-30
references.pmid: 19305395
references.citation: Blood. 2002 Dec 15;100(13):4512-20
references.pmid: 12393628
references.citation: J Clin Invest. 2007 Sep;117(9):2517-25
references.pmid: 17786242
references.citation: Lung. 2005 Mar-Apr;183(2):123-38
references.pmid: 16026016
references.citation: Immunity. 2011 Sep 23;35(3):323-35
references.pmid: 21943488
references.citation: J Exp Med. 2012 Apr 9;209(4):653-60
references.pmid: 22430490
references.citation: Blood. 2003 Nov 1;102(9):3302-10
references.pmid: 12869510
references.citation: J Exp Med. 2010 Apr 12;207(4):823-36
references.pmid: 20351058
references.citation: J Exp Med. 1999 Nov 15;190(10):1417-26
references.pmid: 10562317
references.citation: Nature. 1998 Mar 19;392(6673):245-52
references.pmid: 9521319
references.citation: Blood. 2008 Nov 1;112(9):3671-8
references.pmid: 18713944
references.citation: J Biol Chem. 1995 May 5;270(18):10618-24
references.pmid: 7737999
references.citation: J Immunol. 1999 Jun 1;162(11):6641-9
references.pmid: 10352281
references.citation: J Exp Med. 2005 May 16;201(10):1647-57
references.pmid: 15897278
references.citation: J Exp Med. 2010 Jun 7;207(6):1261-71
references.pmid: 20479117
references.citation: J Immunol. 2000 Dec 1;165(11):6037-46
references.pmid: 11086035
references.citation: Mol Biol Rep. 2010 Mar;37(3):1649-55
references.pmid: 19513812
references.citation: Blood. 1997 Aug 15;90(4):1458-70
references.pmid: 9269763
references.citation: J Exp Med. 1993 Sep 1;178(3):1067-76
references.pmid: 8102389
references.citation: J Exp Med. 2010 Jun 7;207(6):1247-60
references.pmid: 20479116
references.citation: Am J Respir Cell Mol Biol. 2000 Aug;23(2):204-12
references.pmid: 10919987
references.citation: J Exp Med. 2009 Dec 21;206(13):3115-30
references.pmid: 20008528
references.citation: Blood. 2012 Jun 14;119(24):5742-9
references.pmid: 22535664
references.citation: Genes Dev. 2000 Jan 15;14(2):163-76
references.pmid: 10652271
references.citation: Science. 2008 Nov 14;322(5904):1097-100
references.pmid: 19008445
references.citation: Nat Immunol. 2011 Jun;12(6):485-91
references.pmid: 21739671
references.citation: J Exp Med. 2010 Jun 7;207(6):1283-92
references.pmid: 20479118
references.citation: Immunity. 2008 Sep 19;29(3):497-510
references.pmid: 18789730
references.citation: Eur J Immunol. 1999 Mar;29(3):1004-13
references.pmid: 10092105
references.citation: Sci Transl Med. 2012 Jan 18;4(117):117ra7
references.pmid: 22261031
references.citation: PLoS One. 2011;6(10):e25660
references.pmid: 22065991
references.citation: J Immunol. 1993 Dec 1;151(11):6535-45
references.pmid: 7504023
references.citation: Nat Immunol. 2009 Dec;10(12):1237-44
references.pmid: 19915624
references.citation: Semin Immunol. 2005 Aug;17(4):273-83
references.pmid: 15953735
references.citation: J Virol. 2011 May;85(9):4085-94
references.pmid: 21345961
references.citation: Blood. 2012 May 31;119(22):5182-90
references.pmid: 22510877
references.citation: J Exp Med. 2010 Nov 22;207(12):2703-17
references.pmid: 20975040
references.citation: Eur J Immunol. 2011 Sep;41(9):2619-31
references.pmid: 21739428
references.citation: Immunity. 2012 Jul 27;37(1):60-73
references.pmid: 22795876
references.citation: Cell. 1998 Jun 26;93(7):1159-70
references.pmid: 9657149
references.citation: Curr Opin Cell Biol. 2000 Oct;12(5):563-8
references.pmid: 10978890
references.citation: Respir Res. 2009 May 13;10:36
references.pmid: 19439069
references.citation: Immunol Rev. 2010 Mar;234(1):199-212
references.pmid: 20193020
references.citation: Am J Respir Cell Mol Biol. 1996 Nov;15(5):600-10
references.pmid: 8918367
references.citation: J Immunol. 2011 Oct 1;187(7):3488-92
references.pmid: 21880979
references.citation: J Immunol. 1998 Sep 1;161(5):2223-31
references.pmid: 9725215
references.citation: Immunity. 2009 Jul 17;31(1):158-69
references.pmid: 19592276
references.citation: J Immunol. 2005 Dec 15;175(12):7905-15
references.pmid: 16339526
references.citation: Blood. 2010 Sep 9;116(10):1685-97
references.pmid: 20530286
references.citation: Nat Med. 2010 Feb;16(2):224-7
references.pmid: 20081864
references.citation: Clin Exp Immunol. 2007 Jul;149(1):162-70
references.pmid: 17403056
references.citation: Annu Rev Immunol. 2012;30:1-22
references.pmid: 22136168
references.citation: J Exp Med. 2012 May 7;209(5):935-45
references.pmid: 22547651
references.citation: J Clin Invest. 2011 Jun;121(6):2254-63
references.pmid: 21537083