title: Detection and quantification of neurotransmitters in dialysates.
abstract: Sensitive analytical methods are needed for the separation and quantification of neurotransmitters obtained in microdialysate studies. This unit describes methods that permit quantification of nanomolar concentrations of monoamines and their metabolites (high-performance liquid chromatography [HPLC] electrochemical detection), acetylcholine (HPLC-coupled to an enzyme reactor), and amino acids (HPLC-fluorescence detection, capillary electrophoresis with laser-induced fluorescence detection).
authors.affiliation: National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA.
authors.forename: Agustin
authors.initials: A
authors.lastname: Zapata
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Chefer
authors.forename: Sandrine
authors.initials: S
authors.lastname: Parrot
authors.forename: Luc
authors.initials: L
authors.lastname: Denoroy
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015314:Dialysis Solutions / Q000737:chemistry* / Q000378:metabolism*; D055664:Electrochemical Techniques; D019075:Electrophoresis, Capillary; D005453:Fluorescence; D006801:Humans; D007834:Lasers; D017551:Microdialysis / Q000379:methods; D018377:Neurotransmitter Agents / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D015314:Dialysis Solutions; D018377:Neurotransmitter Agents