title: Retinol-encapsulated water-soluble succinated chitosan nanoparticles for antioxidant applications.
abstract: The aim of this study was to stabilize all-trans-retinol (RE) by complexification with chitosan derivatives through H-bonding. Succinated chitosan (CHI-succ) with three different degrees (5, 10, 20 mol%) of succinylation were synthesized to form complexes with RE. Various weight ratios (w/w) of CHI-succ/RE complexes were prepared and characterized to produce stable complexes in nanometer size. The CHI-succ(0.20)/RE complex with approximate 250 nm in diameter was obtained using a CHI-succ(0.20) concentration of 0.005% (w/v) in double deionized water with various contents of RE. From fine-tuning the degree of succinylation and the weight ratio of the CHI-succ and RE, the formation of supramolecular complexes simultaneously improved water solubility and stability of RE. The cell viability of CHI-succ polymers and their RE complexes in 3T3 cells were all>85% relative to the control. The antioxidant ability of the CHI-succ(0.20)/RE complexes was significantly greater than that of pure RE using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay (p<0.01).
authors.affiliation: Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.
authors.forename: Shih-Jer
authors.initials: SJ
authors.lastname: Huang
authors.forename: Shou-Li
authors.initials: SL
authors.lastname: Sun
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D006860:Hydrogen Bonding; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D012995:Solubility; D019802:Succinic Acid / Q000737:chemistry*; D014801:Vitamin A / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D014867:Water / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D002214:Capsules; D016166:Free Radical Scavengers; D010851:Picrates; D014867:Water; D014801:Vitamin A; D048271:Chitosan; D019802:Succinic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl