title: Increasing the production yield of recombinant protein in transgenic seeds by expanding the deposition space within the intracellular compartment.
abstract: Seeds must maintain a constant level of nitrogen in order to germinate. When recombinant proteins are produced while endogenous seed protein expression is suppressed, the production levels of the foreign proteins increase to compensate for the decreased synthesis of endogenous proteins. Thus, exchanging the production of endogenous seed proteins for that of foreign proteins is a promising approach to increase the yield of foreign recombinant proteins. Providing a space for the deposition of recombinant protein in the intracellular compartment is critical, at this would lessen any competition in this region between the endogenous seed proteins and the introduced foreign protein. The production yields of several recombinant proteins have been greatly increased by this strategy.
authors.affiliation: Functional Transgenic Crop Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Japan. takaiwa@nias.affrc.go.jp
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
mesh_terms: D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods*; D042541:Intracellular Space / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins
keywords: PSV; RNA interference; glutelin; molecular farming; prolamin; protein body; recombinant protein; seed protein
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