title: Ubiquitin-like protein from human placental extract exhibits collagenase activity.
abstract: An aqueous extract of human placenta exhibits strong gelatinase/collagenase activity in zymography. 2-D gel electrophoresis of the extract with gelatin zymography in the second dimension displayed a single spot, identified as ubiquitin-like component upon MALDI/TOF MS/MS analysis. Immunoblot indicated presence of ubiquitin and absence of collagenase in the extract. Collagenase activity of the ubiquitin-like component was confirmed from the change in solubility of collagen in aqueous buffer, degradation of collagen by size-exclusion HPLC and atomic force microscopy. Quantification with DQ-gelatin showed that the extract contains 0.04 U/ml of collagenase activity that was inhibited up to 95% by ubiquitin antibody. Ubiquitin from bovine erythrocytes demonstrated mild collagenase activity. Bioinformatics studies suggest that placental ubiquitin and collagenase follow structurally divergent evolution. This thermostable intrinsic collagenase activity of placental extract might have wide physiological relevance in degrading and remodeling collagen as it is used as a drug for wound healing and pelvic inflammatory diseases.
authors.affiliation: Division of Structural Biology and Bioinformatics, Council of Scientific and Industrial Research-Indian Institute of Chemical Biology, Calcutta, West Bengal, India.
authors.forename: Debashree
authors.initials: D
authors.lastname: De
authors.forename: Piyali
authors.initials: P
authors.lastname: Datta Chakraborty
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Mitra
authors.forename: Kanika
authors.initials: K
authors.lastname: Sharma
authors.forename: Somnath
authors.initials: S
authors.lastname: Mandal
authors.forename: Aneesha
authors.initials: A
authors.lastname: Das
authors.forename: Saikat
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: Debasish
authors.initials: D
authors.lastname: Bhattacharyya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D017364:Collagenases / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D018093:Gelatinases / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013045:Species Specificity; D014452:Ubiquitins / Q000737:chemistry / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D010446:Peptide Fragments; D014452:Ubiquitins; D017364:Collagenases; D018093:Gelatinases
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