title: Semiquinone derivative isolated from Bacillus sp. INM-1 protects cellular antioxidant enzymes from γ-radiation-induced renal toxicity.
abstract: This study was focused to evaluate protection of indigenous antioxidant system of mice against gamma radiation-induced oxidative stress using a semiquinone (SQGD)-rich fraction isolated from Bacillus sp. INM-1. Male C57bl/6 mice were administered SQGD (50 mg/kgb.w.i.p.) 2 h before irradiation (10 Gy) and modulation in antioxidant enzymes activities was estimated at different time intervals and compared with irradiated mice which were not pretreated by SQGD. Compared to untreated controls, SQGD pretreatment significantly (p < 0.05) accelerates superoxide dismutase, catalase, GSH, and glutathione-S-transferase activities. Similarly, significant (p < 0.05) increase in the expression of superoxide dismutase, catalase, GSH, and glutathione-S-transferase was observed in irradiated mice pretreated by SQGD, compared to only irradiated groups. Total antioxidant status equivalent to trolox was estimated in renal tissue of the mice after SQGD administration. Significant ABTS(+) radical formation was observed in H2O2-treated kidney homogenate, due to oxidative stress in the tissue. However, significant decrease in the levels of ABTS(+) radical was observed in kidney homogenate of the mice pretreated with SQGD. Therefore, it can be concluded that SQGD neutralizes oxidative stress by induction of antioxidant enzymes activities and thus improved total antioxidant status in cellular system and hence contributes to radioprotection.
authors.affiliation: Radiation Biosciences Division, Radiation Biotechnology Group, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: S
authors.initials: S
authors.lastname: Mishra
authors.forename: D S K
authors.initials: DS
authors.lastname: Reddy
authors.forename: V S
authors.initials: VS
authors.lastname: Jamwal
authors.forename: D D
authors.initials: DD
authors.lastname: Bansal
authors.forename: D D
authors.initials: DD
authors.lastname: Patel
authors.forename: P
authors.initials: P
authors.lastname: Malhotra
authors.forename: A K
authors.initials: AK
authors.lastname: Gupta
authors.forename: P K
authors.initials: PK
authors.lastname: Singh
authors.forename: S
authors.initials: S
authors.lastname: Jawed
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001407:Bacillus / Q000737:chemistry*; D016227:Benzoquinones / Q000494:pharmacology* / Q000627:therapeutic use; D002374:Catalase / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D011833:Radiation Injuries, Experimental / Q000201:enzymology / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D016166:Free Radical Scavengers; D011837:Radiation-Protective Agents; C025232:semiquinone radicals; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione
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