title: CD30L/CD30 is critical for maintenance of IL-17A-producing γδ T cells bearing Vγ6 in mucosa-associated tissues in mice.
abstract: CD30 ligand (CD30L, CD153), a member of the tumor necrosis factor (TNF) superfamily, and its receptor CD30 are important for differentiation and activation of CD4(+) T helper type 17 (Th17) cells. In this report, we demonstrate that the interleukin 17A (IL-17A)-producing γδ T cells normally developed in the fetal thymus, whereas Vγ1(-)Vγ4(-) γδ T cells expressed Vγ6/Vδ1 gene transcript selectively decreased in mucosa-associated tissues in naive CD30KO or CD30LKO mice. Moreover, CD30 and CD30L were expressed preferentially by Vγ1(-)Vγ4(-) γδ T cells in naive mice. The bacteria clearance was attenuated by the impaired response of the IL-17A-producing γδ T cells and decreased infiltration of neutrophils in CD30KO or CD30LKO mice. In vivo administration of agonistic anti-CD30 monoclonal antibody restored the ability of protection against Listeria monocytogenes by enhancing Vγ1(-)Vγ4(-) γδ T cells producing IL-17A not only in wild-type but also CD30LKO mice. Taken together, it appears that CD30L/CD30 signaling plays an important role in the maintenance and activation of IL-17A-producing γδ T cells presumably bearing Vγ6 in the mucosa-associated tissues of mice.
authors.affiliation: 1] Department of Immunology, China Medical University, Shenyang, China [2] Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan [3] Research Center for Advanced Immunology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.forename: K
authors.initials: K
authors.lastname: Shibata
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: H
authors.initials: H
authors.lastname: Muta
authors.forename: E R
authors.initials: ER
authors.lastname: Podack
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshikai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000494:pharmacology; D053285:CD30 Ligand / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006706:Homeostasis / Q000187:drug effects; D020381:Interleukin-17 / Q000378:metabolism; D017730:Ki-1 Antigen / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D008088:Listeriosis / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009092:Mucous Membrane / Q000276:immunology* / Q000382:microbiology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D053285:CD30 Ligand; D020381:Interleukin-17; D017730:Ki-1 Antigen; D016692:Receptors, Antigen, T-Cell, gamma-delta; C000589613:T-cell receptor Vgamma6, mouse; C505775:Tnfsf8 protein, mouse