title: The effect of hydrogen gas on a mouse bilateral common carotid artery occlusion.
abstract: In recent studies, molecular hydrogen selectively reduced the levels of hydroxyl radicals in vitro and exerted a therapeutic anti-oxidant activity in a rat middle cerebral artery occlusion model. The aim of this study was to investigate the effect of hydrogen gas on a mouse bilateral common carotid artery occlusion (BCCAO) model. Male C57BL/6J mice were subjected to transient BCCAO with a nontraumatic aneurysm clip. The mice were divided into three groups: sham, BCCAO, and BCCAO treated with 1.3 % hydrogen gas. Cerebral blood flow (CBF) in the cortex was measured sequentially for both hemispheres with a non--invasive and noncontact laser Doppler blood perfusion imager during the procedure. Vital signs were also recorded. Oxidative stress evaluated by measuring the level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), neuronal injury in the hippocampal CA1 sector, and brain water content were assessed 24 h after ischemia. The hydrogen gas treatment had no significant effect on vital signs or CBF values. However, the reduction of the expression of 8-OHdG, the decrease in the neuronal injury in the hippocampal CA1 sector, and the attenuation in brain water content were observed in hydrogen-treated mice. In conclusion, hydrogen gas might be effective in a mouse BCCAO model.
authors.affiliation: Department of Neurosurgery, National Defense Medical College, Saitama, Japan. naval.kimi@gmail.com
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Nagatani
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeuchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Otani
authors.forename: Kojiro
authors.initials: K
authors.lastname: Wada
authors.forename: Masanori
authors.initials: M
authors.lastname: Fujita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nawashiro
authors.forename: Shoichi
authors.initials: S
authors.lastname: Tachibana
authors.forename: Katsuji
authors.initials: K
authors.lastname: Shima
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000704:Analysis of Variance; D000818:Animals; D001157:Arterial Occlusive Diseases; D001929:Brain Edema / Q000209:etiology / Q000517:prevention & control; D017536:Carotid Artery, Common / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology*; D002560:Cerebrovascular Circulation / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D006624:Hippocampus / Q000473:pathology; D006859:Hydrogen / Q000627:therapeutic use*; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015424:Reperfusion; D014463:Ultrasonography
chemical_list: D006859:Hydrogen; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine