title: The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding.
abstract: Resistance (R) proteins recognize pathogen avirulence (Avr) proteins by direct or indirect binding and are multidomain proteins generally carrying a nucleotide binding (NB) and a leucine-rich repeat (LRR) domain. Two NB-LRR protein-coding genes from rice (Oryza sativa), RGA4 and RGA5, were found to be required for the recognition of the Magnaporthe oryzae effector AVR1-CO39. RGA4 and RGA5 also mediate recognition of the unrelated M. oryzae effector AVR-Pia, indicating that the corresponding R proteins possess dual recognition specificity. For RGA5, two alternative transcripts, RGA5-A and RGA5-B, were identified. Genetic analysis showed that only RGA5-A confers resistance, while RGA5-B is inactive. Yeast two-hybrid, coimmunoprecipitation, and fluorescence resonance energy transfer-fluorescence lifetime imaging experiments revealed direct binding of AVR-Pia and AVR1-CO39 to RGA5-A, providing evidence for the recognition of multiple Avr proteins by direct binding to a single R protein. Direct binding seems to be required for resistance as an inactive AVR-Pia allele did not bind RGA5-A. A small Avr interaction domain with homology to the Avr recognition domain in the rice R protein Pik-1 was identified in the C terminus of RGA5-A. This reveals a mode of Avr protein recognition through direct binding to a novel, non-LRR interaction domain.
authors.affiliation: INRA, UMR 385 Biologie et Génétique des Interactions Plante-Parasite, F-34398 Montpellier, France.
authors.forename: Stella
authors.initials: S
authors.lastname: Cesari
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Thilliez
authors.forename: Cécile
authors.initials: C
authors.lastname: Ribot
authors.forename: Véronique
authors.initials: V
authors.lastname: Chalvon
authors.forename: Corinne
authors.initials: C
authors.lastname: Michel
authors.forename: Alain
authors.initials: A
authors.lastname: Jauneau
authors.forename: Susana
authors.initials: S
authors.lastname: Rivas
authors.forename: Ludovic
authors.initials: L
authors.lastname: Alaux
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanzaki
authors.forename: Yudai
authors.initials: Y
authors.lastname: Okuyama
authors.forename: Jean-Benoit
authors.initials: JB
authors.lastname: Morel
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Fournier
authors.forename: Didier
authors.initials: D
authors.lastname: Tharreau
authors.forename: Ryohei
authors.initials: R
authors.lastname: Terauchi
authors.forename: Thomas
authors.initials: T
authors.lastname: Kroj
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions; D015151:Immunoblotting; D020082:Magnaporthe / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D020033:Protein Isoforms
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