title: Cytotoxicity evaluation of three resin-based sealers on an L929 cell line.
abstract: BACKGROUND
  Endodontic sealers usually come in contact with adjacent tissues and their biocompatibility is key in a successful treatment. The purpose of this study was to assess the cytotoxicity of three resin-based sealers, namely AH Plus, EndoREZ, and Epiphany in set and fresh states on an L929 cell line.
  
  
  METHODS
  In this in vitro experimental study, the materials were mixed according to the manufacturers' instructions, and were divided into two groups, fresh and set. The elutes of materials were prepared separately and were incubated with L929 fibroblasts for 1 hour, 24 hours, and 72 hours. Pulp Canal Sealer and Dulbecco's Modified Eagle Medium (DMEM) served as positive and negative controls respectively. Cell viability was evaluated by MTT assay ([3-4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide succinate), after 1 hour, 24 hours, and 72 hours. The data were analyzed by analysis of variance (ANOVA), and Tukey multiple comparison test.
  
  
  RESULTS
  After 1 hour, fresh Epiphany and fresh AH Plus were significantly more cytotoxic than their set samples. No significant difference was perceived between cytotoxicity of fresh state of sealers and positive control, or between set state and negative control. After 24 hours, both fresh and set samples of all materials were significantly more cytotoxic than the negative control group, and were less cytotoxic than the positive control group. After 72 hours, the fresh and set samples of all materials were as cytotoxic as the positive control group. At each time point, no significant difference was perceived among different materials in terms of cell viability.
  
  
  CONCLUSIONS
  The observed differences among the cytotoxicity of AH Plus, EndoREZ, and Epiphany did not reach a significant level at comparable time points after exposure.
authors.affiliation: Department of Endodontic, Iranian Center of Endodontic Research, Tehran, Iran.
authors.forename: Hengameh
authors.initials: H
authors.lastname: Ashraf
authors.forename: Nima
authors.initials: N
authors.lastname: Moradimajd
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Mozayeni
authors.forename: Omid
authors.initials: O
authors.lastname: Dianat
authors.forename: Faranak
authors.initials: F
authors.lastname: Mahjour
authors.forename: Zahra
authors.initials: Z
authors.lastname: Yadegari
keywords: Cytotoxicity; MTT assay; fibroblasts; root canal sealer
references.citation: Oral Health. 1998 Dec;88(12):37-9
references.pmid: 10323136
references.citation: Int Endod J. 2003 Apr;36(4):256-61
references.pmid: 12702119
references.citation: J Endod. 2007 Jan;33(1):24-7
references.pmid: 17185123
references.citation: J Endod. 2008 Sep;34(9):1085-8
references.pmid: 18718370
references.citation: J Oral Rehabil. 2003 Apr;30(4):426-9
references.pmid: 12631168
references.citation: Dent Mater J. 1999 Mar;18(1):76-86
references.pmid: 10786150
references.citation: J Endod. 2006 Feb;32(2):130-2
references.pmid: 16427461
references.citation: J Endod. 2011 Feb;37(2):246-9
references.pmid: 21238811
references.citation: Int Endod J. 1996 Mar;29(2):76-83
references.pmid: 9206428
references.citation: J Endod. 2009 Feb;35(2):225-8
references.pmid: 19166778
references.citation: J Endod. 2006 Oct;32(10):989-92
references.pmid: 16982280
references.citation: Oral Surg Oral Med Oral Pathol. 1993 Mar;75(3):357-61
references.pmid: 8469549
references.citation: Int Endod J. 2010 Feb;43(2):148-53
references.pmid: 20078704
references.citation: J Endod. 2010 Jan;36(1):40-4
references.pmid: 20003933
references.citation: Int Endod J. 2002 Feb;35(2):153-8
references.pmid: 11843970
references.citation: Dent Res J (Isfahan). 2012 Jan;9(1):86-92
references.pmid: 22363369
references.citation: J Mater Sci Mater Med. 2004 Jul;15(7):767-71
references.pmid: 15387412
references.citation: J Endod. 2010 Jan;36(1):110-4
references.pmid: 20003946
references.citation: Biomaterials. 1991 Oct;12(8):737-40
references.pmid: 1799649
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Dec;108(6):952-60
references.pmid: 19913728
references.citation: Dent Res J (Isfahan). 2011 Dec;8(Suppl 1):S21-9
references.pmid: 23372592
references.citation: Eur J Oral Sci. 2004 Apr;112(2):182-7
references.pmid: 15056117
references.citation: Int Endod J. 2011 Jul;44(7):626-34
references.pmid: 21306404
references.citation: Int Endod J. 2008 Jan;41(1):72-7
references.pmid: 17931390
references.citation: Int Endod J. 2008 Dec;41(12):1072-8
references.pmid: 19133096
references.citation: Aust Endod J. 2001 Apr;27(1):12-21
references.pmid: 11481874
references.citation: J Endod. 2002 Nov;28(11):784-6
references.pmid: 12470025
references.citation: Int Endod J. 2006 Jun;39(6):464-71
references.pmid: 16674741
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Jul;92(1):89-92
references.pmid: 11458251
references.citation: Dent Mater. 2003 Jan;19(1):25-31
references.pmid: 12498893
references.citation: Int Endod J. 2007 May;40(5):329-37
references.pmid: 17309743
references.citation: Clin Oral Investig. 1997 Feb;1(1):5-11
references.pmid: 9552811