title: Sitagliptin, sitagliptin and metformin, or sitagliptin and amitriptyline attenuate streptozotocin-nicotinamide induced diabetic neuropathy in rats.
abstract: Diabetic neuropathies are a family of nerve disorders caused by diabetes. Symptoms of the disease include nerve palsy, mononeuropathy, mononeuropathy multiplex, diabetic amyotrophy, painful polyneuropathy, autonomic neuropathy, and thoracoabdominal neuropathy. In this study, type 2 diabetes in rats was induced with nicotinamide-streptozotocin. Drug treatment was initiated on the d 15, with the combination regimen of metformin, pioglitazone and glimipiride or metformin and sitagliptin or sitagliptin, amitriptyline and sitagliptin and led to significantly improved glycemic control, increased grip strength and paw jumping response on d 21, 28 and 35 (P < 0.001). Significant increases in blood protein levels and decreases in urinary protein levels were observed in the animals treated with the different regimens on d 21, 28 and 35 (P < 0.001). Combined treatment of streptozotocin and nicotinamide caused marked degeneration of nerve cells, while administration of metformin and sitagliptin showed tissue regeneration and no body weight gain. In conclusion, treatment with sitagliptin and sitagliptin combined with metformin or amitriptyline results in no body weight gain, but causes an increase in grip strength and pain sensitivity, exhibits neural protection, and reverses the alteration of biochemical parameters in rats with streptozotocin-nicotinamide induced type 2 diabetes.
authors.affiliation: Department of Pharmacology, Gyan Vihar School of Pharmacy, Suresh Gyan Vihar University, Jagatpura, Jaipur (Rajasthan) 302025, India;
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Sharma
authors.forename: Akash
authors.initials: A
authors.lastname: Sharma
authors.forename: Rita
authors.initials: R
authors.lastname: Kumari
authors.forename: Kunal
authors.initials: K
authors.lastname: Kishore
authors.forename: Divya
authors.initials: D
authors.lastname: Sharma
authors.forename: Bharthu Parthsarthi
authors.initials: BP
authors.lastname: Srinivasan
authors.forename: Ashok
authors.initials: A
authors.lastname: Sharma
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Singh
authors.forename: Samir
authors.initials: S
authors.lastname: Gaur
authors.forename: Vijay Singh
authors.initials: VS
authors.lastname: Jatav
authors.forename: Prashant
authors.initials: P
authors.lastname: Sharma
authors.forename: Varnika
authors.initials: V
authors.lastname: Srivastava
authors.forename: Sneha
authors.initials: S
authors.lastname: Joshi
authors.forename: Megha
authors.initials: M
authors.lastname: Joshi
authors.forename: Prashant Kumar
authors.initials: PK
authors.lastname: Dhakad
authors.forename: Davender Singh
authors.initials: DS
authors.lastname: Kanawat
authors.forename: Akanksha
authors.initials: A
authors.lastname: Mishra
authors.forename: Anil
authors.initials: A
authors.lastname: Sharma
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Singh
authors.forename: Ravinder Pal
authors.initials: RP
authors.lastname: Singh
authors.forename: Himmat Singh
authors.initials: HS
authors.lastname: Chawda
authors.forename: Rambir
authors.initials: R
authors.lastname: Singh
authors.forename: Sachin Kumar
authors.initials: SK
authors.lastname: Raikwar
authors.forename: Muneem Kumar
authors.initials: MK
authors.lastname: Kurmi
authors.forename: Pankaj
authors.initials: P
authors.lastname: Khatri
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Agarwal
authors.forename: Arshee
authors.initials: A
authors.lastname: Munajjam
keywords: amitriptyline; diabetic neuropathy; glimipiride; metformin; nicotinamide-streptozotocin; pioglitazone; sitagliptin
references.citation: J Diabetes Sci Technol. 2010 Jan 01;4(1):228-9
references.pmid: 20167189
references.citation: J Neurosci Methods. 2006 Mar 15;151(2):131-8
references.pmid: 16125783
references.citation: Diabetes Care. 2006 Dec;29(12):2638-43
references.pmid: 17130197
references.citation: Diabetologia. 1990 Sep;33(9):561-8
references.pmid: 2253834
references.citation: Clin Pharmacol Ther. 2005 Dec;78(6):675-88
references.pmid: 16338283
references.citation: Hypertension. 2010 Oct;56(4):728-33
references.pmid: 20679179
references.citation: Diabetes Metab Res Rev. 2010 Oct;26(7):540-9
references.pmid: 20824678
references.citation: Eur J Pharm Sci. 2009 Dec 8;38(5):433-44
references.pmid: 19765654
references.citation: Neurology. 1987 Apr;37(4):589-96
references.pmid: 2436092
references.citation: Clin Sci (Lond). 2011 Jan;120(2):73-80
references.pmid: 20795946
references.citation: Regul Pept. 2011 Jan 17;166(1-3):48-54
references.pmid: 20728477
references.citation: Histol Histopathol. 2004 Oct;19(4):1345-51
references.pmid: 15375776
references.citation: Cancer Res. 2005 Feb 15;65(4):1325-34
references.pmid: 15735018
references.citation: Am J Med. 1999 Aug 30;107(2B):1S
references.pmid: 10484038
references.citation: Plant Foods Hum Nutr. 2007 Mar;62(1):25-9
references.pmid: 17226069
references.citation: Regul Pept. 2005 Jun 15;128(2):159-65
references.pmid: 15780435
references.citation: Diabetes. 1998 Feb;47(2):224-9
references.pmid: 9519717
references.citation: Clin Chem. 1975 Nov;21(12):1795-8
references.pmid: 1182999
references.citation: Diabetes Care. 1994 Nov;17(11):1337-40
references.pmid: 7821176
references.citation: N Engl J Med. 2001 Sep 27;345(13):971-80
references.pmid: 11575290
references.citation: Diabetes. 1997 Apr;46(4):622-31
references.pmid: 9075802
references.citation: Diabetes. 1975 Jan;24(1):44-53
references.pmid: 1122063