title: Inosine-specific cleavage of RNA for microarray analysis of RNA A-to-I editing targets.
abstract: Dysregulations of RNA A-to-I editing are associated with developmental defects in mouse and human diseases. Although several methods of identifying RNA A-to-I editing sites are currently available, most of the critical editing targets responsible for the important biological functions of adenosine deaminases that act on RNA (ADARs) remain unknown. Here we report a modified I-specific cleavage method that improves the quality of the RNA product. Preliminary microarray comparison of RNAs subjected to I-specific cleavage or mock digestion reported 165 genes that showed more than 0.2-fold reductions due to the cleavage. Six of the 165 genes were randomly selected for further verification, and three were verified to be targets of I-specific cleavage. This method may provide an alternative method of identifying novel RNA A-to-I editing sites using a microarray and facilitate the inquiry into the roles of RNA A-to-I editing in various biological processes.
authors.affiliation: Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
authors.forename: Hurng-Wern
authors.initials: HW
authors.lastname: Huang
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Wang
authors.forename: Chiu-Jin
authors.initials: CJ
authors.lastname: Lu
authors.forename: Shaio-Wen
authors.initials: SW
authors.lastname: Chen
authors.forename: Szu-Cheng
authors.initials: SC
authors.lastname: Yi
authors.forename: Hay-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Chung-Lung
authors.initials: CL
authors.lastname: Cho
authors.forename: Cheng-Hsuan
authors.initials: CH
authors.lastname: Wu
authors.forename: Jyuer-Ger
authors.initials: JG
authors.lastname: Yang
authors.forename: Chao-Neng
authors.initials: CN
authors.lastname: Tseng
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D007288:Inosine / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D046228:Microarray Analysis; D059367:RNA Cleavage; D017393:RNA Editing
chemical_list: D007288:Inosine; D000243:Adenosine Deaminase