title: Development of a singleplex PCR assay for rapid identification and differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and hybrids.
abstract: A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.
authors.affiliation: Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Feng
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Fu
authors.forename: Bo
authors.initials: B
authors.lastname: Ling
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Wanqing
authors.initials: W
authors.lastname: Liao
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Yao
mesh_terms: D003453:Cryptococcosis / Q000175:diagnosis* / Q000382:microbiology; D056285:Cryptococcus gattii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal
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