title: Bacterial strains isolated from PCB-contaminated sediments and their use for bioaugmentation strategy in microcosms.
abstract: This study was focused on the characterization of 15 bacterial strains isolated from long-term PCB-contaminated sediment located at the Strážsky canal in eastern part of Slovakia, in the surroundings of a former PCB producer. PCB-degrading strains were isolated and identified as Microbacterium oleivorans, Stenotrophomonas maltophilia, Brevibacterium sp., Ochrobactrum anthropi, Pseudomonas mandelii, Rhodococcus sp., Achromobacter xylosoxidans, Stenotrophomonas sp., Ochrobactrum sp., Pseudomonas aeruginosa, and Starkeya novella by the 16S rRNA gene sequence phylogenetic analysis. This study presents a newly isolated bacterial strain S. novella with PCB-degrading ability in liquid medium as well as in sediment. For A. xylosoxidans, the bphA gene was identified. The best growth ability in the presence of all sole carbon sources (biphenyl and PCBs vapor) was obtained for Ochrobactrum sp. and Rhodococcus sp. Uncultured Achromobacter sp. showed the highest potential for bioaugmentation of PCB-contaminated sediment.
authors.affiliation: Faculty of Chemical and Food Technology, Department of Biochemical Technology, Institute of Biotechnology and Food Science, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Hana
authors.initials: H
authors.lastname: Dudášová
authors.forename: Lucia
authors.initials: L
authors.lastname: Lukáčová
authors.forename: Slavomíra
authors.initials: S
authors.lastname: Murínová
authors.forename: Andrea
authors.initials: A
authors.lastname: Puškárová
authors.forename: Domenico
authors.initials: D
authors.lastname: Pangallo
authors.forename: Katarína
authors.initials: K
authors.lastname: Dercová
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000737:chemistry*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D018154:Slovakia; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D007506:Iron-Sulfur Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D010105:Oxygenases; C074161:biphenyl-2,3-dioxygenase
keywords: Bioaugmentation; Biodegradation; PCBs; Starkeya novella; bphA gene