title: Determination of plaque viability following a single brushing with commercial toothpastes.
abstract: OBJECTIVE
  The purpose of this study was to determine the viability of dental plaque at 12 hours following brushing with Colgate Great Regular Flavor (0.83% MFP), Crest Pro-Health (0.454% SnF), and Colgate Total Clean Mint Toothpaste (0.3% triclosan/ copolymer/0.22% NaF).
  
  
  METHODS
  Thirty-two healthy volunteers from Bangkok, Thailand were chosen to participate in a double-blind clinical crossover study for three one-week treatment periods. They were randomly assigned to three groups using different sequences of test toothpastes. The plaque samples were collected at baseline (prior to brushing) and 12 hours after brushing with assigned toothpastes at the scheduled appointments. Scores and percentages of plaque viability were examined under a fluorescent microscope using a mixture of 40 microl of green and red fluorescent dyes, and were statistically analyzed using a two-way ANOVA.
  
  
  RESULTS
  Plaque viability scores and percent plaque viability at baseline with all test toothpastes showed no significant differences with a p-value > 0.05. The post-treatment mean values of plaque viability scores for Colgate Great Regular, Crest Pro-Health, and Colgate Total 12 hours after brushing were 1.39, 3.01, and 3.37, and represented 90.06, 49.80, and 40.77 percent viable plaque, respectively. The Analysis of Variance (ANOVA) showed statistically significant differences when comparing Colgate Great Regular and Crest Pro-Health, Colgate Great Regular and Colgate Total, and Crest Pro-Health and Colgate Total using Tukey's test with a p-value < 0.001.
  
  
  CONCLUSIONS
  Colgate Total, containing triclosan/copolymer/NaF as the active ingredients, is effective and superior to Crest Pro-Health and Colgate Regular in controlling the viability of oral bacteria in dental plaque.
authors.affiliation: Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. dtpvp@mahidol.ac.th
authors.forename: Petcharat
authors.initials: P
authors.lastname: Kraivaphan
authors.forename: Cholticha
authors.initials: C
authors.lastname: Amornchat
authors.forename: Terdphong
authors.initials: T
authors.lastname: Triratana
mesh_terms: D000328:Adult; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects*; D002327:Cariostatic Agents / Q000627:therapeutic use*; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000382:microbiology*; D003774:Dental Plaque Index; D004311:Double-Blind Method; D005260:Female; D005456:Fluorescent Dyes; D005459:Fluorides / Q000627:therapeutic use; D006801:Humans; D008297:Male; D050296:Microbial Viability / Q000187:drug effects; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D010710:Phosphates / Q000627:therapeutic use; D012824:Silicic Acid / Q000627:therapeutic use; D012969:Sodium Fluoride / Q000627:therapeutic use; D014002:Tin Fluorides / Q000627:therapeutic use; D014099:Toothbrushing / Q000379:methods*; D014100:Toothpastes / Q000627:therapeutic use*; D014260:Triclosan / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D000891:Anti-Infective Agents, Local; D002327:Cariostatic Agents; C510825:Crest Pro-Health Dentifrice; D005456:Fluorescent Dyes; D010710:Phosphates; D014002:Tin Fluorides; D014100:Toothpastes; C546060:hydrated silica gel-based toothpaste; D012824:Silicic Acid; C012980:fluorophosphate; D014260:Triclosan; D012969:Sodium Fluoride; D005459:Fluorides