title: Do blood contamination and haemostatic agents affect microtensile bond strength of dual cured resin cement to dentin?
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effects of blood contamination and haemostatic agents such as Ankaferd Blood Stopper (ABS) and hydrogen peroxide (H2O2) on the microtensile bond strength between dual cured resin cement-dentin interface.
  
  
  METHODS
  Twelve pressed lithium disilicate glass ceramics were luted to flat occlusal dentin surfaces with Panavia F under the following conditions:
  
  
  METHODS
  no contamination, Group Blood: blood contamination, Group ABS: ABS contamination Group H2O2: H2O2 contamination. The specimens were sectioned to the beams and microtensile testing was carried out. Failure modes were classified under stereomicroscope. Two specimens were randomly selected from each group, and SEM analyses were performed.
  
  
  RESULTS
  There were significant differences in microtensile bond strengths (µTBS) between the control and blood-contaminated groups (p<0.05), whereas there were no significant differences found between the control and the other groups (p>0.05).
  
  
  CONCLUSIONS
  Contamination by blood of dentin surface prior to bonding reduced the bond strength between resin cement and the dentin. Ankaferd Blood Stoper and H2O2 could be used safely as blood stopping agents during cementation of all-ceramics to dentin to prevent bond failure due to blood contamination.
authors.affiliation: Department of Prosthodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey. dtkeremkilic@hotmail.com
authors.forename: Kerem
authors.initials: K
authors.lastname: Kilic
authors.forename: Soley
authors.initials: S
authors.lastname: Arslan
authors.forename: Goknil Alkan
authors.initials: GA
authors.lastname: Demetoglu
authors.forename: Gokmen
authors.initials: G
authors.lastname: Zararsiz
authors.forename: Bulent
authors.initials: B
authors.lastname: Kesim
mesh_terms: D001769:Blood; D001840:Dental Bonding / Q000379:methods*; D019232:Dental Restoration Failure; D003804:Dentin / Q000737:chemistry*; D004866:Equipment Contamination; D006490:Hemostatics / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D033081:Shear Strength; D013223:Statistics as Topic; D013499:Surface Properties; D013718:Tensile Strength
chemical_list: D006490:Hemostatics; C438843:Panavia-Fluoro; D010936:Plant Extracts; D019279:Resin Cements; C539751:ankaferd blood stopper; D006861:Hydrogen Peroxide
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