title: Selective isotope labelling of leucine residues by using α-ketoacid precursor compounds.
abstract: You can have one without the other: A new metabolic precursor compound can selectively introduce (13)C and (2)H patterns at leucine residues in proteins in cell-based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high-molecular-weight proteins by NMR spectroscopy.
authors.affiliation: Institute of Organic Chemistry, University of Vienna, Währingerstrasse 38, 1090 Vienna, Austria. roman.lichtenecker@univie.ac.at
authors.forename: Roman J
authors.initials: RJ
authors.lastname: Lichtenecker
authors.forename: Nicolas
authors.initials: N
authors.lastname: Coudevylle
authors.forename: Robert
authors.initials: R
authors.lastname: Konrat
authors.forename: Walther
authors.initials: W
authors.lastname: Schmid
mesh_terms: D002247:Carbon Isotopes; D007553:Isotope Labeling; D007651:Keto Acids / Q000737:chemistry*; D007930:Leucine / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D007651:Keto Acids; D011522:Protons; D007930:Leucine