title: E-selectin mediated adhesion and migration of endothelial colony forming cells is enhanced by SDF-1α/CXCR4.
abstract: OBJECTIVE
  Endothelial-colony forming cells (ECFCs) can be readily expanded from human umbilical cord blood and can facilitate repair of endothelial injury. E-selectin and SDF-1α are produced following endothelial injury and can regulate endothelial progenitor homing. Mechanisms of vascular repair specific to the mode of injury have not been well described in homogenous cell populations such as ECFCs and are needed for development of more effective vascular repair strategies.
  
  
  RESULTS
  Lipopolysaccharide (LPS)-induced endotoxic injury to mature human umbilical vein endothelial cells (HUVEC) was compared with hypoxic and radiation injury. E-selectin expression in HUVEC cells is markedly increased (208-fold) following LPS-induced injury and facilitates increased ECFC adhesion and migration function in vitro. SDF-1α expression remains unchanged in LPS-treated HUVEC cells but increases more than 2 fold in fibroblasts undergoing similar endotoxic injury. SDF-1α induces expression of E-selectin ligands on ECFCs and facilitates greater E-selectin-mediated adhesion and migration of ECFCs in a CXCR4-dependent manner. Induction of E-selectin expression in HUVECs following hypoxic or radiation injury is negligible, however, while SDF-1α is increased markedly following hypoxia, highlighting injury-specific synergism between mediators of vascular repair.
  
  
  CONCLUSIONS
  E-selectin mediates adhesion and migration of ECFCs following endotoxic endothelial injury. SDF-1α augments E-selectin mediated ECFC adhesion and migration in a CXCR4-dependent manner.
authors.affiliation: Bone Marrow Transplantation Centre, Medical College of Zhejiang University, Hangzhou, Zhujiang Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Li
authors.forename: Gina M
authors.initials: GM
authors.lastname: Graziani
authors.forename: Lionel
authors.initials: L
authors.lastname: Filion
authors.forename: David S
authors.initials: DS
authors.lastname: Allan
mesh_terms: D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*
chemical_list: C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D019040:E-Selectin; D008070:Lipopolysaccharides; D019718:Receptors, CXCR4
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