title: Fabrication of a multi-layer three-dimensional scaffold with controlled porous micro-architecture for application in small intestine tissue engineering.
abstract: Various methods can be employed to fabricate scaffolds with characteristics that promote cell-to-material interaction. This report examines the use of a novel technique combining compression molding with particulate leaching to create a unique multi-layered scaffold with differential porosities and pore sizes that provides a high level of control to influence cell behavior. These cell behavioral responses were primarily characterized by bridging and penetration of two cell types (epithelial and smooth muscle cells) on the scaffold in vitro. Larger pore sizes corresponded to an increase in pore penetration, and a decrease in pore bridging. In addition, smaller cells (epithelial) penetrated further into the scaffold than larger cells (smooth muscle cells). In vivo evaluation of a multi-layered scaffold was well tolerated for 75 d in a rodent model. This data shows the ability of the components of multi-layered scaffolds to influence cell behavior, and demonstrates the potential for these scaffolds to promote desired tissue outcomes in vivo.
authors.affiliation: Tengion, Inc., Winston Salem, NC, USA. toyin.knight@tengion.com
authors.forename: Toyin
authors.initials: T
authors.lastname: Knight
authors.forename: Joydeep
authors.initials: J
authors.lastname: Basu
authors.forename: Elias A
authors.initials: EA
authors.lastname: Rivera
authors.forename: Thomas
authors.initials: T
authors.lastname: Spencer
authors.forename: Deepak
authors.initials: D
authors.lastname: Jain
authors.forename: Richard
authors.initials: R
authors.lastname: Payne
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002460:Cell Line; D002465:Cell Movement; D004847:Epithelial Cells / Q000378:metabolism; D007421:Intestine, Small; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D016062:Porosity; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials
keywords: multi-layer scaffold; pore size; porosity; small intestine; tissue engineering
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