title: Three novel antimicrobial peptides from the skin of Rana shuchinae.
abstract: Intensive studies have demonstrated that there are many antimicrobial peptides in amphibian skins. Three novel antimicrobial peptides were identified from the skin of the frog, Rana shuchinae. They are named shuchins 3-5. Their sequences were determined as KAYSMPRCKGGFRAVMCWL-NH2, KAYSTPRCKGLFRALMCWL-NH2, and KAYSMPRCKYLFRAVLCWL-NH2 by Edman degradation and mass spectrometry analysis, respectively. They are composed of 19 amino acids (aa) with unique sequences. BLAST search indicated that they showed no similarity to any known peptides or proteins. They are a novel family of antimicrobial peptide. These peptides showed antimicrobial activities against all of tested microorganisms including Gram-positive bacteria, Gram-negative bacteria and fungi. The cDNAs encoding precursors of these peptides were cloned from the skin cDNA library of R. shuchinae. The precursors are composed of 64 amino acid residues including predicted signal peptides, acidic spacer peptides, and mature antimicrobial peptides. The current work identified a novel antimicrobial peptide family.
authors.affiliation: The Department of Breast Surgery of the First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei 230022, Anhui, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Pei
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Benzhong
authors.initials: B
authors.lastname: Wang
authors.forename: Hanjin
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005658:Fungi / Q000187:drug effects; D015723:Gene Library; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006461:Hemolysis; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D011898:Ranidae; D012867:Skin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary