title: Immunobiologic and antiinflammatory properties of a bark extract from Ampelozizyphus amazonicus Ducke.
abstract: Ampelozizyphus amazonicus is used in the treatment and prevention of malaria. The effect of an aqueous extract from this plant (SART) on the immune response was investigated by measuring immunoglobulin production induced by immunization with the antigen TNP-Ficoll in Plasmodium chabaudi-infected mice. SART treatment increased antigen-specific IgM and IgG levels in TNP-Ficoll-immunized mice. The B cell response during malarial infection was also modified by SART. There was an increase in total serum IgM and IgG and a decrease in the percentage of splenic plasma cells (CD138+ cells) in P. chabaudi-infected, SART-treated animals. SART (1, 3 or 10 mg/kg, p.o.) and the reference drug dexamethasone (5 mg/kg) were also tested in carrageenan-induced leukocyte migration to the subcutaneous air pouch (SAP). All SART doses significantly reduced leukocyte migration into the SAP. The protein concentration resulting from extravasation into the peritoneum was also significantly reduced. Our data indicate that SART possesses immunomodulatory properties, inducing an in vivo modification of the B lymphocyte response and anti-inflammatory properties, which are partly due to a reduction in cell migration and are most likely due to an inhibition of the production of inflammatory mediators. Preliminary HPLC-ESI-MS/MS analysis of SART shows a complex saponin profile with deprotonated molecule [M-H](-) ions in the range of m/z 800-1000.
authors.affiliation: Departamento de Imunologia, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, CCS Bloco I, 2° andar, 21941-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Ligia Maria Torres
authors.initials: LM
authors.lastname: Peçanha
authors.forename: Patricia Dias
authors.initials: PD
authors.lastname: Fernandes
authors.forename: Tatiana Jotha-Mattos
authors.initials: TJ
authors.lastname: Simen
authors.forename: Danilo Ribeiro
authors.initials: DR
authors.lastname: de Oliveira
authors.forename: Priscilla Vanessa
authors.initials: PV
authors.lastname: Finotelli
authors.forename: Marina Vieira Agostinho
authors.initials: MV
authors.lastname: Pereira
authors.forename: Fernanda Ferreira
authors.initials: FF
authors.lastname: Barboza
authors.forename: Thays da Silva
authors.initials: Tda S
authors.lastname: Almeida
authors.forename: Stephanie
authors.initials: S
authors.lastname: Carvalhal
authors.forename: Anna Paola Trindade Rocha
authors.initials: AP
authors.lastname: Pierucci
authors.forename: Gilda Guimarães
authors.initials: GG
authors.lastname: Leitão
authors.forename: Luca
authors.initials: L
authors.lastname: Rastrelli
authors.forename: Anna Lisa
authors.initials: AL
authors.lastname: Piccinelli
authors.forename: Suzana Guimarães
authors.initials: SG
authors.lastname: Leitão
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects*; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008288:Malaria / Q000188:drug therapy* / Q000276:immunology / Q000469:parasitology; D051379:Mice; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D016788:Plasmodium chabaudi / Q000276:immunology* / Q000472:pathogenicity; D027823:Rhamnaceae / Q000737:chemistry*
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010936:Plant Extracts
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