title: Immunohistochemical study of doublecortin and nucleostemin in canine brain.
abstract: Finding a marker of neural stem cells remains a medical research priority. It was reported that the proteins doublecortin and nucleostemin were related with stem/progenitor cells in central nervous system. The aim of the present immunohistochemical study was to evaluate the expression of these proteins and their pattern of distribution in canine brain, including age-related changes, and in non-nervous tissues. We found that doublecortin had a more specific expression pattern, related with neurogenesis and neuronal migration, while nucleostemin was expressed in most cells of almost every tissue studied. The immunolabeling of both proteins decreased with age. We may conclude that nucleostemin is not a specific marker of stem/progenitor cells in the dog. Doublecortin, however, is not an exclusive marker of neural stem cells, but also of neuronal precursors.
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain. eleonora.denevi@gmail.com
authors.forename: E
authors.initials: E
authors.lastname: De Nevi
authors.forename: P
authors.initials: P
authors.lastname: Marco-Salazar
authors.forename: D
authors.initials: D
authors.lastname: Fondevila
authors.forename: E
authors.initials: E
authors.lastname: Blasco
authors.forename: L
authors.initials: L
authors.lastname: Pérez
authors.forename: M
authors.initials: M
authors.lastname: Pumarola
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D004285:Dogs; D000089622:Doublecortin Domain Proteins; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009479:Neuropeptides / Q000096:biosynthesis*; D009687:Nuclear Proteins / Q000096:biosynthesis*
chemical_list: D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins
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