title: No association between MGP rs1800802 polymorphism and stenosis of the coronary artery.
abstract: OBJECTIVE
  Matrix Gla protein (MGP) was originally isolated from bone but it is known to be expressed in several tissues including kidney, lung, heart, cartilage and vascular smooth muscle cells (VSMC) of the blood vessel wall. Since it inhibits calcification in subendothelial space of vessels thus, we evaluated the association of rs1800802(T > C) polymorphism and stenosis of the coronary artery.
  
  
  METHODS
  Cross-sectional case-control.
  
  
  METHODS
  One hundred eighty two subjects recruited on the basis of study protocol from who underwent coronary angiography. The controls (n=70) had normal coronary arteries (up to 5% stenosis). The patients (n=112) subdivided into three subgroups; single-vessel disease (SVD), two-vessel disease (2VD) and three-vessel disease (3VD) based on the number of stenosed coronary vessels (at least 50% stenosis). rs1800802 (T > C) polymorphism was determined by PCR-RFLP technique.
  
  
  RESULTS
  Genotype distribution was not significant between control and patient groups. In addition, there were no significant differences between rs1800802 (T > C) frequency and gender (P=.092), and also patient subgroups (one-, two- and three vessel disease) (P=.840).
  
  
  CONCLUSIONS
  We concluded that rs1800802 (T > C) polymorphism within the MGP promoter is not related to stenosis of the coronary artery.
authors.affiliation: Biochemistry Department, Tehran University of Medical Sciences, Tehran 141761351, Iran. A-Roustazadeh@razi.tums.ac.ir
authors.forename: Abazar
authors.initials: A
authors.lastname: Roustazadeh
authors.forename: Mohammad
authors.initials: M
authors.lastname: Najafi
authors.forename: Abdollah
authors.initials: A
authors.lastname: Amirfarhangi
authors.forename: Issa
authors.initials: I
authors.lastname: Nourmohammadi
mesh_terms: D000328:Adult; D000368:Aged; D054458:Amplified Fragment Length Polymorphism Analysis; D002135:Calcium-Binding Proteins / Q000235:genetics*; D016022:Case-Control Studies; D023921:Coronary Stenosis / Q000235:genetics*; D003430:Cross-Sectional Studies; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D000097568:Matrix Gla Protein
chemical_list: D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D005819:Genetic Markers; D000097568:Matrix Gla Protein
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