title: MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
abstract: The purpose of this study was to describe the microRNA (miRNA) expression profiles of neutrophils and their precursors from the initiation of granulopoiesis in the bone marrow to extravasation and accumulation in skin windows. We analyzed three different cell populations from human bone marrow, polymorphonuclear neutrophil (PMNs) from peripheral blood, and extravasated PMNs from skin windows using the Affymetrix 2.0 platform. Our data reveal 135 miRNAs differentially regulated during bone marrow granulopoiesis. The majority is differentially regulated between the myeloblast/promyelocyte (MB/PM) and myelocyte/metamyelocyte (MC/MM) stages of development. These 135 miRNAs were divided into six clusters according to the pattern of their expression. Several miRNAs demonstrate a pronounced increase or reduction at the transition between MB/PM and MC/MM, which is associated with cell cycle arrest and the initiation of terminal differentiation. Seven miRNAs are differentially up-regulated between peripheral blood PMNs and extravasated PMNs and only one of these (miR-132) is also differentially regulated during granulopoiesis. The study indicates that several different miRNAs participate in the regulation of normal granulopoiesis and that miRNAs might also regulate activities of extravasated neutrophils. The data present the miRNA profiles during the development and activation of the neutrophil granulocyte in healthy humans and thus serves as a reference for further research of normal and malignant granulocytic development.
authors.affiliation: The Granulocyte Research Laboratory, Department of Hematology, National University Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Larsen
authors.forename: Christoffer
authors.initials: C
authors.lastname: Hother
authors.forename: Mattias
authors.initials: M
authors.lastname: Häger
authors.forename: Corinna C
authors.initials: CC
authors.lastname: Pedersen
authors.forename: Kim
authors.initials: K
authors.lastname: Theilgaard-Mönch
authors.forename: Niels
authors.initials: N
authors.lastname: Borregaard
authors.forename: Jack B
authors.initials: JB
authors.lastname: Cowland
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D038042:Myelopoiesis / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*
chemical_list: D035683:MicroRNAs
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