title: Evidence in support of RNA-mediated inhibition of phosphatidylserine-dependent HIV-1 Gag membrane binding in cells.
abstract: The matrix domain promotes plasma-membrane-specific binding of HIV-1 Gag through interaction with an acidic lipid phosphatidylinositol-(4,5)-bisphosphate. In in vitro systems, matrix-bound RNA suppresses Gag interactions with phosphatidylserine, an acidic lipid prevalent in various cytoplasmic membranes, thereby enhancing the lipid specificity of the matrix domain. Here we provide in vitro and cell-based evidence supporting the idea that this RNA-mediated suppression occurs in cells and hence is a physiologically relevant mechanism that prevents Gag from binding promiscuously to phosphatidylserine-containing membranes.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
authors.forename: Vineela
authors.initials: V
authors.lastname: Chukkapalli
authors.forename: Jingga
authors.initials: J
authors.lastname: Inlora
authors.forename: Gabrielle C
authors.initials: GC
authors.lastname: Todd
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D010718:Phosphatidylserines / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012313:RNA / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D010718:Phosphatidylserines; D054301:gag Gene Products, Human Immunodeficiency Virus; D012313:RNA
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