title: Research resource: identification of novel coregulators specific for thyroid hormone receptor-β2.
abstract: Thyroid hormone receptors (TRs) are expressed as a series of interrelated isoforms that perform distinct biological roles. The TRβ2 isoform is found predominantly in the hypothalamus, pituitary, retina, and cochlea and displays unique transcriptional properties relative to the other TR isoforms. To more fully understand the isoform-specific biological and molecular properties of TRβ2, we have identified a series of previously unrecognized proteins that selectively interact with TRβ2 compared with the more widely expressed TRβ1. Several of these proteins preferentially enhance the transcriptional activity of TRβ2 when coexpressed in cells and are likely to represent novel, isoform-specific coactivators. Additional proteins were also identified in our screen that bind equally to TRβ1 and TRβ2 and may function as isoform-independent auxiliary proteins for these and/or other nuclear receptors. We propose that a combination of isoform-specific recruitment and tissue-specific expression of these newly identified coregulator candidates serves to customize TR function for different biological purposes in different cell types.
authors.affiliation: Department of Microbiology, University of California at Davis, Davis, CA 95616, USA.
authors.forename: Johnnie B
authors.initials: JB
authors.lastname: Hahm
authors.forename: Martin L
authors.initials: ML
authors.lastname: Privalsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002457:Cell Extracts; D002460:Cell Line; D002853:Chromatography, Liquid; D004247:DNA / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D037042:Thyroid Hormone Receptors beta / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D002457:Cell Extracts; D009687:Nuclear Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D037042:Thyroid Hormone Receptors beta; D004247:DNA
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