title: Retinal function assessment of trypan blue versus indocyanine green assisted internal limiting membrane peeling during macular hole surgery.
abstract: OBJECTIVE
  To compare multifocal electroretinography (mfERG) retinal function and the anatomical and visual outcomes of macular hole surgery performed with indocyanine green (ICG) or trypan blue (TB).
  
  
  METHODS
  Prospective, randomized study.
  
  
  METHODS
  Twenty-five eyes of 24 patients.
  
  
  METHODS
  Patients underwent a pars plana vitrectomy with removal of the internal limiting membrane. In 14 eyes, internal limiting membrane visualization during macular hole repair was performed using TB, and ICG was used in 11 eyes. The examination protocol (performed before surgery and at 3 weeks, 3 months, 6 months, and 12 months after surgery) included optical coherence tomography, mfERG (mfERG-103 hexagons), and assessment of best corrected visual acuity (BCVA) and contrast sensitivity (CS).
  
  
  RESULTS
  Closure of macular hole was achieved in 100% of the cases. In the TB group, P1 amplitude and implicit time improved significantly at 12 months after surgery (P < 0.05), whereas in the ICG group, significant improvement occurred at both 6 (P < 0.05) and 12 months (P < 0.01). BCVA improved significantly in both groups at 6 and 12 months (P < 0.01). Both groups also showed a statistically significant CS improvement at spatial frequency of 6 cycles per degree (P = 0.01) 1 year postoperatively. At 12 months, improvement of P1 amplitude and implicit time, BCVA, and CS was not different between groups.
  
  
  CONCLUSIONS
  In this study, the use of TB or ICG appears to yield similar improvement in terms of BCVA, CS, and mfERG amplitude and implicit time changes at 12 months.
authors.affiliation: Department of Ophthalmology, Saint-Sacrement Hospital, Laval University, Quebec, QC. claudinebellerive@hotmail.com
authors.forename: Claudine
authors.initials: C
authors.lastname: Bellerive
authors.forename: Benoit
authors.initials: B
authors.lastname: Cinq-Mars
authors.forename: Mélissa
authors.initials: M
authors.lastname: Louis
authors.forename: Yvon
authors.initials: Y
authors.lastname: Tardif
authors.forename: Marcelle
authors.initials: M
authors.lastname: Giasson
authors.forename: Kathy
authors.initials: K
authors.lastname: Francis
authors.forename: Marc
authors.initials: M
authors.lastname: Hébert
mesh_terms: D000368:Aged; D001485:Basement Membrane / Q000473:pathology / Q000601:surgery*; D004396:Coloring Agents; D015350:Contrast Sensitivity / Q000502:physiology; D004596:Electroretinography; D058450:Endotamponade; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage; D005500:Follow-Up Studies; D006801:Humans; D007208:Indocyanine Green; D008297:Male; D008875:Middle Aged; D016684:Prone Position; D011446:Prospective Studies; D012160:Retina / Q000502:physiology*; D012167:Retinal Perforations / Q000175:diagnosis / Q000503:physiopathology / Q000601:surgery*; D041623:Tomography, Optical Coherence; D014343:Trypan Blue; D014792:Visual Acuity / Q000502:physiology; D014821:Vitrectomy
chemical_list: D004396:Coloring Agents; D005466:Fluorocarbons; C042852:perflutren; D014343:Trypan Blue; D007208:Indocyanine Green