title: Efficient capture of pathogens with a zeolite matrix.
abstract: We show that a peptide linker sequence expressed as part of a fusion with protein G from Streptococcus binds to natural zeolite giving a complex to which a suitable antibody shows affinity binding. As a consequence, addition of the CRY104 antibody specific for an external surface epitope of Cryptosporidium allows the capture of this protozoan pathogen at high efficiency with the advantage of rapid concentration from water samples. The natural zeolite with the specific antibody attached was incorporated into a single and double zeolite column system. The results reported here show that it is possible to capture Cryptosporidium oocysts with outstanding efficiency (>90 %) from water and water incorporating QC-MUD using a size-sorted natural zeolite. The natural zeolite matrix not only allows for high flow rates but also for flexibility of column design and volume of water for sample collection. The system is versatile and it is possible to prepare columns with more than one specific surface antibody to allow the capture of one or two pathogens simultaneously.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, and Environmental Biotechnology CRC, Macquarie University, Sydney, Australia. anwar.sunna@mq.edu.au
authors.forename: Anwar
authors.initials: A
authors.lastname: Sunna
authors.forename: Fei
authors.initials: F
authors.lastname: Chi
authors.forename: Peter L
authors.initials: PL
authors.lastname: Bergquist
mesh_terms: D000913:Antibodies, Protozoan / Q000378:metabolism*; D002448:Cell Adhesion; D002846:Chromatography, Affinity / Q000379:methods*; D003458:Cryptosporidium / Q000302:isolation & purification*; D010274:Parasitology / Q000379:methods*; D011485:Protein Binding; D013048:Specimen Handling / Q000379:methods*; D014867:Water / Q000469:parasitology*; D017641:Zeolites
chemical_list: D000913:Antibodies, Protozoan; D014867:Water; D017641:Zeolites
references.citation: Protein Expr Purif. 2007 Jul;54(1):110-6
references.pmid: 17408966
references.citation: Nat Biotechnol. 1997 Mar;15(3):269-72
references.pmid: 9062928
references.citation: Int J Parasitol. 2006 Jun;36(7):811-9
references.pmid: 16696982
references.citation: Parasitol Res. 2013 Feb;112(2):467-71
references.pmid: 23052785
references.citation: Anal Biochem. 2005 Oct 15;345(2):250-7
references.pmid: 16125126
references.citation: J Microbiol Methods. 2004 Sep;58(3):321-5
references.pmid: 15279936
references.citation: J Biol Chem. 1986 Aug 5;261(22):10240-7
references.pmid: 3733709
references.citation: Emerg Infect Dis. 1999 Jul-Aug;5(4):579-84
references.pmid: 10458969
references.citation: J Appl Bacteriol. 1995 Jun;78(6):575-85
references.pmid: 7615412
references.citation: J Food Prot. 2013 Feb;76(2):307-13
references.pmid: 23433379
references.citation: Annu Rev Public Health. 1997;18:135-61
references.pmid: 9143715
references.citation: Anal Biochem. 2003 Jul 1;318(1):80-5
references.pmid: 12782034
references.citation: Vet Parasitol. 2004 Dec 9;126(1-2):219-34
references.pmid: 15567586
references.citation: J Microbiol Methods. 2001 Jun;45(2):69-76
references.pmid: 11311391
references.citation: J Chromatogr B Biomed Sci Appl. 1999 Feb 5;722(1-2):33-53
references.pmid: 10068132
references.citation: Cytometry. 2000 Nov 1;41(3):216-22
references.pmid: 11042619
references.citation: Protein Expr Purif. 1994 Dec;5(6):569-76
references.pmid: 7858426
references.citation: Parasitology. 2007 Mar;134(Pt 3):309-23
references.pmid: 17096874
references.citation: Vet Parasitol. 2007 Nov 30;150(1-2):13-7
references.pmid: 17954011
references.citation: J Microbiol Methods. 2005 Oct;63(1):73-88
references.pmid: 15893393
references.citation: Exp Parasitol. 2010 Jan;124(1):61-79
references.pmid: 19501088
references.citation: AIDS. 1993 Mar;7(3):349-54
references.pmid: 8471197
references.citation: Water Res. 2011 Dec 15;45(20):6603-14
references.pmid: 22048017
references.citation: N Biotechnol. 2013 Jun 25;30(5):485-92
references.pmid: 23238030
references.citation: Cytometry A. 2003 Feb;51(2):79-86
references.pmid: 12541282
references.citation: Appl Environ Microbiol. 2002 Dec;68(12):5952-5
references.pmid: 12450815
references.citation: J Water Health. 2007 Mar;5(1):1-38
references.pmid: 17402277
references.citation: Analyst. 2007 Nov;132(11):1075-82
references.pmid: 17955140