title: In vitro effect of human serum and fetal calf serum on CD4+ T cells proliferation in response to myelin oligodendrocyte glycoprotein (MOG) in correlation with RBP/TTR ratio in multiple sclerotic patients.
abstract: Myelin oligodendrocyte glycoprotein (MOG) is one of the autoantigens used in evaluation of the CD4(+) T cells proliferation response in multiple sclerotic patients. In cell culture, human serum (HS) is one of the promising substitutions for fetal calf serum (FCS) that can induce different autoreactivity of T cells and fluctuation of autoantibody production from B cells. Because of immunomodulatory function of vitamin A, we examined the effect of HS and FCS on CD4(+) T cells proliferation in response to MOG in correlation with serum retinol-binding protein (RBP)/transthyretin (TTR) ratio, as an indirect way to assess vitamin A status in multiple sclerotic patients. Patients' peripheral blood mononuclear cells were isolated and cultured in the presence of MOG as well as FCS and HS both separately and together. Cell proliferation was evaluated using BrdU kit. Serum RBP and TTR levels were measured by ELISA kit. FCS and HS increase CD4(+) T cell proliferation. RBP/TTR ratio has significant negative correlation with cell proliferation in the presence of MOG, HS, and FCS. HS with FCS provides an appropriate medium for autoreactivity and proliferation of CD4(+) T cells. Vitamin A has a crucial role in regulation of this pathway.
authors.affiliation: Cellular and Molecular Nutrition Department, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Niyaz Mohammadzadeh
authors.initials: NM
authors.lastname: Honarvar
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Harrirchian
authors.forename: Fariba
authors.initials: F
authors.lastname: Koohdani
authors.forename: Fereydoon
authors.initials: F
authors.lastname: Siassi
authors.forename: Sima
authors.initials: S
authors.lastname: Jafari Rad
authors.forename: Mina
authors.initials: M
authors.lastname: Abdolahi
authors.forename: Sama
authors.initials: S
authors.lastname: Bitarafan
authors.forename: Eisa
authors.initials: E
authors.lastname: Salehi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Sahraian
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Eshraghian
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboor-Yarghi
mesh_terms: D000328:Adult; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology*; D011228:Prealbumin / Q000032:analysis; D012177:Retinol-Binding Proteins / Q000032:analysis; D014801:Vitamin A / Q000378:metabolism
chemical_list: D003470:Culture Media; D063308:Myelin-Oligodendrocyte Glycoprotein; D011228:Prealbumin; D012177:Retinol-Binding Proteins; D014801:Vitamin A
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