title: Preparation and characterization of poly(pluronic-co-L-lactide) nanofibers for tissue engineering.
abstract: In this work, a new kind of biodegradable poly(pluronic-co-L-lactide) (Pluronic-PLLA copolymers) was successfully prepared by melt-polycondensation method from L-lactide, Pluronic and isophorone diisocyanate (IPDI). The obtained copolymers were characterized by (1)H NMR, FT-IR, X-ray, and TGA/DTA. Meanwhile, three-dimensional (3-D) porous scaffolds based on Pluronic-PLLA were prepared by the electrospinning method, the factors of concentration, flow rate and voltage that influence the formation of the Pluronic-PLLA nanofibers were studied and the structure of Pluronic-PLLA nanofibers were investigated by scanning electron microscopy (SEM). MTT results revealed that the Pluronic-PLLA scaffolds had good biocompatibility and nontoxicity. Morphological study using fluorescence micrographs and scanning electron microscopy showed that in vitro osteoblast cell culture demonstrated the electrospun Pluronic-PLLA composite scaffolds could provide a suitable environment for good cell attachment. These results suggested that such Pluronic-PLLA nanofibers membranes might have prospective applications in tissue engineering field.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Qingfa
authors.initials: Q
authors.lastname: Guo
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Ding
authors.forename: Dexia
authors.initials: D
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Tang
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D018786:Bone Substitutes / Q000138:chemical synthesis / Q000633:toxicity; D002448:Cell Adhesion; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D018360:Crystallography, X-Ray; D055664:Electrochemical Techniques; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000502:physiology; D011060:Poloxalene / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000633:toxicity; D011091:Polyesters / Q000138:chemical synthesis* / Q000633:toxicity; D013818:Thermogravimetry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; C430808:poly(lactide-pluronic)copolymer; D011060:Poloxalene