title: Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing.
abstract: The objective of this study was to investigate the compositional profiles and microbial shifts of oral microbiota during head-and-neck radiotherapy. Bioinformatic analysis based on 16S rRNA gene pyrosequencing was performed to assess the diversity and variation of oral microbiota of irradiated patients. Eight patients with head and neck cancers were involved in this study. For each patient, supragingival plaque samples were collected at seven time points before and during radiotherapy. A total of 147,232 qualified sequences were obtained through pyrosequencing and bioinformatic analysis, representing 3,460 species level operational taxonomic units (OTUs) and 140 genus level taxa. Temporal variations were observed across different time points and supported by cluster analysis based on weighted UniFrac metrics. Moreover, the low evenness of oral microbial communities in relative abundance was revealed by Lorenz curves. This study contributed to a better understanding of the detailed characterization of oral bacterial diversity of irradiated patients.
authors.affiliation: Department of Endodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors.forename: Yue-Jian
authors.initials: YJ
authors.lastname: Hu
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Yun-Tao
authors.initials: YT
authors.lastname: Jiang
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Xia
authors.forename: Zi-Sheng
authors.initials: ZS
authors.lastname: Tang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Liang
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Huang
mesh_terms: D000190:Actinomyces / Q000145:classification / Q000528:radiation effects; D000191:Actinomycetaceae / Q000145:classification / Q000528:radiation effects; D042401:Alcaligenaceae / Q000145:classification / Q000528:radiation effects; D001419:Bacteria / Q000145:classification* / Q000528:radiation effects; D002206:Capnocytophaga / Q000145:classification / Q000528:radiation effects; D056568:Carnobacteriaceae / Q000145:classification / Q000528:radiation effects; D019295:Computational Biology; D003773:Dental Plaque / Q000382:microbiology*; D005500:Follow-Up Studies; D058834:Gemella / Q000145:classification / Q000528:radiation effects; D006258:Head and Neck Neoplasms / Q000532:radiotherapy*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008875:Middle Aged; D009343:Neisseria / Q000145:classification / Q000528:radiation effects; D018720:Prevotella / Q000145:classification / Q000528:radiation effects; D011423:Propionibacteriaceae / Q000145:classification / Q000528:radiation effects; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013291:Streptococcus / Q000145:classification / Q000528:radiation effects; D014678:Veillonella / Q000145:classification / Q000528:radiation effects
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
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