title: Historical shifts in Brazilian P. falciparum population structure and drug resistance alleles.
abstract: Previous work suggests that Brazilian Plasmodium falciparum has limited genetic diversity and a history of bottlenecks, multiple reintroductions due to human migration, and clonal expansions. We hypothesized that Brazilian P. falciparum would exhibit clonal structure. We examined isolates collected across two decades from Amapá, Rondônia, and Pará state (n = 190). By examining more microsatellites markers on more chromosomes than previous studies, we hoped to define the extent of low diversity, linkage disequilibrium, bottlenecks, population structure, and parasite migration within Brazil. We used retrospective genotyping of samples from the 1980s and 1990s to explore the population genetics of SP resistant dhfr and dhps alleles. We tested an existing hypothesis that the triple mutant dhfr mutations 50R/51I/108N and 51I/108N/164L developed in southern Amazon from a single origin of common or similar parasites. We found that Brazilian P. falciparum had limited genetic diversity and isolation by distance was rejected, which suggests it underwent bottlenecks followed by migration between sites. Unlike Peru, there appeared to be gene flow across the Brazilian Amazon basin. We were unable to divide parasite populations by clonal lineages and pairwise FST were common. Most parasite diversity was found within sites in the Brazilian Amazon, according to AMOVA. Our results challenge the hypothesis that triple mutant alleles arose from a single lineage in the Southern Amazon. SP resistance, at both the double and triple mutant stages, developed twice and potentially in different regions of the Brazilian Amazon. We would have required samples from before the 1980s to describe how SP resistance spread across the basin or describe the complex internal migration of Brazilian parasites after the colonization efforts of past decades. The Brazilian Amazon basin may have sufficient internal migration for drug resistance reported in any particular region to rapidly spread to other parts of basin under similar drug pressure.
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Griffing
authors.forename: Giselle M Rachid
authors.initials: GM
authors.lastname: Viana
authors.forename: Tonya
authors.initials: T
authors.lastname: Mixson-Hayden
authors.forename: Sankar
authors.initials: S
authors.lastname: Sridaran
authors.forename: Mohammad Tauqeer
authors.initials: MT
authors.lastname: Alam
authors.forename: Alexandre Macedo
authors.initials: AM
authors.lastname: de Oliveira
authors.forename: John W
authors.initials: JW
authors.lastname: Barnwell
authors.forename: Ananias A
authors.initials: AA
authors.lastname: Escalante
authors.forename: Marinete Marins
authors.initials: MM
authors.lastname: Povoa
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Udhayakumar
mesh_terms: D000483:Alleles; D005075:Biological Evolution; D001938:Brazil; D004351:Drug Resistance / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D006801:Humans; D018895:Microsatellite Repeats; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
chemical_list: D015800:Protozoan Proteins; D013762:Tetrahydrofolate Dehydrogenase
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