title: ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse.
abstract: ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS) has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI.
authors.affiliation: Department of Medical Biology, Turgut Ozal University School of Medicine, Ankara, Turkey. kdemircan@yahoo.com
authors.forename: Kadir
authors.initials: K
authors.lastname: Demircan
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Takigawa
authors.forename: Vehap
authors.initials: V
authors.lastname: Topcu
authors.forename: Serpil
authors.initials: S
authors.lastname: Erdogan
authors.forename: Fatma
authors.initials: F
authors.lastname: Ucar
authors.forename: Ferah
authors.initials: F
authors.lastname: Armutcu
authors.forename: M Ramazan
authors.initials: MR
authors.lastname: Yigitoglu
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirohata
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D000071097:ADAMTS1 Protein; D000071116:ADAMTS5 Protein; D000071119:ADAMTS9 Protein; D000818:Animals; D010450:Endopeptidases / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011509:Proteoglycans / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism*; D013904:Thoracic Vertebrae / Q000293:injuries / Q000378:metabolism
chemical_list: D011509:Proteoglycans; D010450:Endopeptidases; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; D000071116:ADAMTS5 Protein; D000071119:ADAMTS9 Protein; C486323:Adamts1 protein, mouse; C498489:Adamts5 protein, mouse; C545434:Adamts9 protein, mouse; C083641:aggrecanase