title: Whole-cell patch-clamp recording of nicotinic acetylcholine receptors in adult Brugia malayi muscle.
abstract: Lymphatic filariasis is a debilitating disease caused by clade III parasites like Brugia malayi and Wuchereria bancrofti. Current recommended treatment regimen for this disease relies on albendazole, ivermectin and diethylcarbamazine, none of which targets the nicotinic acetylcholine receptors in these parasitic nematodes. Our aim therefore has been to develop adult B. malayi for electrophysiological recordings to aid in characterizing the ion channels in this parasite as anthelmintic target sites. In that regard, we recently demonstrated the amenability of adult B. malayi to patch-clamp recordings and presented results on the single-channel properties of nAChR in this nematode. We have built on this by recording whole-cell nAChR currents from adult B. malayi muscle. Acetylcholine, levamisole, pyrantel, bephenium and tribendimidine activated the receptors on B. malayi muscle, producing robust currents ranging from >200 pA to ~1.5 nA. Levamisole completely inhibited motility of the adult B. malayi within 10 min and after 60 min, motility had recovered back to control values.
authors.affiliation: Department Biomedical Science, College of Veterinary Medicine, Iowa State University, Ames, USA. Electronic address: alanr@iastate.edu.
authors.forename: A P
authors.initials: AP
authors.lastname: Robertson
authors.forename: S K
authors.initials: SK
authors.lastname: Buxton
authors.forename: R J
authors.initials: RJ
authors.lastname: Martin
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001598:Bephenium Compounds / Q000494:pharmacology; D017178:Brugia malayi / Q000187:drug effects / Q000502:physiology*; D004594:Electrophysiology; D004605:Elephantiasis, Filarial / Q000469:parasitology*; D005260:Female; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007978:Levamisole / Q000494:pharmacology; D032342:Muscle Cells / Q000187:drug effects / Q000502:physiology; D018722:Nicotinic Agonists / Q000494:pharmacology*; D018408:Patch-Clamp Techniques / Q000379:methods*; D010655:Phenylenediamines / Q000494:pharmacology; D011715:Pyrantel / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*
chemical_list: D000871:Anthelmintics; D001598:Bephenium Compounds; D007473:Ion Channels; D018722:Nicotinic Agonists; D010655:Phenylenediamines; D011978:Receptors, Nicotinic; C056017:tribendimidine; D007978:Levamisole; D011715:Pyrantel; D000109:Acetylcholine
keywords: Acetylcholine receptor; Anthelmintic; Brugia malayi
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