title: Ultrastructure and phylogeny of Ustilago coicis.
abstract: Ustilago coicis causes serious smut on Coix lacryma-jobi in Dayang Town, Jinyun County, Zhejiang Province of China. In this paper, ultrastructural assessments on fungus-host interactions and teliospore development are presented, and molecular phylogenetic analyses have been done to elucidate the phylogenetic placement of the taxon. Hyphal growth within infected tissues was both intracellular and intercellular and on the surface of fungus-host interaction, and the fungal cell wall and the invaginated host plasma membrane were separated by a sheath comprising two distinct layers between the fungal cell wall and the invaginated host plasma membrane. Ornamentation development of teliospore walls was unique as they appeared to be originated from the exosporium. In addition, internal transcribed spacer (ITS) and large subunit (LSU) sequence data showed that U. coicis is closely related to Ustilago trichophora which infects grass species of the genus Echinochloa (Poaceae).
authors.affiliation: Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China. jzzhang@zju.edu.cn
authors.forename: Jing-ze
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Pei-gang
authors.initials: PG
authors.lastname: Guan
authors.forename: Gang
authors.initials: G
authors.lastname: Tao
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ojaghian
authors.forename: Kevin David
authors.initials: KD
authors.lastname: Hyde
mesh_terms: D031742:Coix / Q000235:genetics / Q000382:microbiology* / Q000648:ultrastructure*; D004271:DNA, Fungal / Q000235:genetics*; D006790:Host-Parasite Interactions / Q000502:physiology*; D010802:Phylogeny; D014588:Ustilago / Q000502:physiology* / Q000648:ultrastructure*
chemical_list: D004271:DNA, Fungal
references.citation: J Mol Evol. 1989 Aug;29(2):170-9
references.pmid: 2509717
references.citation: J Agric Food Chem. 2003 Jun 4;51(12):3656-60
references.pmid: 12769541
references.citation: Mycol Res. 2005 Mar;109(Pt 3):342-56
references.pmid: 15912952
references.citation: J Zhejiang Univ Sci B. 2010 Jan;11(1):61-70
references.pmid: 20043353
references.citation: Comput Appl Biosci. 1996 Aug;12(4):357-8
references.pmid: 8902363