title: Efficient enzymatic hydrolysis of the bagasse pulp prepared with active oxygen and MgO-based solid alkali.
abstract: The enzymatic hydrolysis of the bagasse pulp prepared from the treatment process with active oxygen and MgO-based solid alkali was studied. The hydrolysates were tested by IC (ionic chromatography) for the analysis of monosaccharide. Additionally, the changes of pulp before and after hydrolysis were characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), Kajaani cellulose automatic analyzer and atomic force microscopy (AFM) techniques. The results showed that an optimized sugar yield of 82.38% was obtained at the substrate concentration of 5% for 72h with the enzyme dosage of 15IU/g. Furthermore, as the length of the cellulose fiber decreased, the crystallinity of cellulose increased, and more depressions appeared on the surface of pulp after enzymatic hydrolysis.
authors.affiliation: State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China. xietujun2006@163.com
authors.forename: Tujun
authors.initials: T
authors.lastname: Xie
authors.forename: Lu
authors.initials: L
authors.lastname: Lin
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Pang
authors.forename: Junping
authors.initials: J
authors.lastname: Zhuang
authors.forename: Jianbin
authors.initials: J
authors.lastname: Shi
authors.forename: Qiulin
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004798:Enzymes / Q000378:metabolism*; D006868:Hydrolysis; D008277:Magnesium Oxide / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis; D017382:Reactive Oxygen Species / Q000737:chemistry*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D000468:Alkalies; D004798:Enzymes; D009005:Monosaccharides; D017382:Reactive Oxygen Species; D008277:Magnesium Oxide; D002482:Cellulose; C027433:bagasse; D001617:beta-Glucosidase; D002480:Cellulase