title: Structural elucidation of an immunoenhancing heteroglycan isolated from Russula albonigra (Krombh.) Fr.
abstract: A water soluble heteroglycan (PS-II) of average molecular weight ∼1.45 × 10(5)Da was isolated from the aqueous extract of an ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr. Structural characterization of PS-II was carried out using acid hydrolysis, methylation analysis, periodate oxidation, and 1D/2D NMR studies. Structural analysis revealed that PS-II was composed of terminal 2-O-methyl-Fucp, terminal Manp, (1→2)-Fucp, (1→3)-Glcp, (1→3,4)-Glcp, (1→6)-Galp, and (1→2,6)-Galp residues in a relative proportion of approximately 1:1:1:1:1:1:1. The proposed repeating unit of the PS-II had a backbone consisting of two (1→3)-β-d-glucopyranosyl, two (1→6)-α-d-galactopyranosyl, and one (1→2)-α-l-fucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-4 position with terminal 2-O-methyl-α-l-fucopyranosyl and one (1→6)-α-d-galactopyranosyl residue was branched at O-2 position with terminal α-d-mannopyranosyl residue. This PS-II showed in vitro macrophage activation by NO production as well as splenocytes and thymocytes proliferation.
authors.affiliation: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006868:Hydrolysis; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009569:Nitric Oxide / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides; D009569:Nitric Oxide