title: Synthesis of fucosyl-N-acetylglucosamine disaccharides by transfucosylation using α-L-fucosidases from Lactobacillus casei.
abstract: AlfB and AlfC α-l-fucosidases from Lactobacillus casei were used in transglycosylation reactions, and they showed high efficiency in synthesizing fucosyldisaccharides. AlfB and AlfC activities exclusively produced fucosyl-α-1,3-N-acetylglucosamine and fucosyl-α-1,6-N-acetylglucosamine, respectively. The reaction kinetics showed that AlfB can convert 23% p-nitrophenyl-α-l-fucopyranoside into fucosyl-α-1,3-N-acetylglucosamine and AlfC at up to 56% into fucosyl-α-1,6-N-acetylglucosamine.
authors.affiliation: Laboratorio de Bacterias Lácticas y Probióticos, Departamento de Biotecnología de Alimentos, IATA-CSIC, Valencia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Rodríguez-Díaz
authors.forename: Rodrigo J
authors.initials: RJ
authors.lastname: Carbajo
authors.forename: Antonio
authors.initials: A
authors.lastname: Pineda-Lucena
authors.forename: Vicente
authors.initials: V
authors.lastname: Monedero
authors.forename: María J
authors.initials: MJ
authors.lastname: Yebra
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D004187:Disaccharides / Q000096:biosynthesis*; D004795:Enzyme Stability; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D005644:alpha-L-Fucosidase / Q000378:metabolism*
chemical_list: D004187:Disaccharides; C028011:fucosyl alpha 1,3 N-acetylglucosamine; D005644:alpha-L-Fucosidase; D000117:Acetylglucosamine
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