title: Brevibacterium jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood.
abstract: A Gram-staining-positive, aerobic, non-motile bacterium, designated strain SJ5-8(T), was isolated from seau-jeot (shrimp jeotgal), a traditional fermented seafood in South Korea. Cells were non-spore-forming rods showing catalase- and oxidase-positive reactions. Growth of strain SJ5-8(T) was observed at 10-37 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.5-8.5) and in the presence of 0-14% (w/v) NaCl (optimum, 5%). Phylogenetic inference based on 16S rRNA gene sequences showed that the strain formed a tight phyletic lineage with members of the genus Brevibacterium. Strain SJ5-8(T) was most closely related to Brevibacterium yomogidense MN-6-a(T), Brevibacterium daeguense 2C6-41(T) and Brevibacterium salitolerans TRM 415(T) with similarities of 98.9, 97.5 and 97.4%, respectively. The DNA-DNA relatedness values between strain SJ5-8(T) and the type strains of B. yomogidense, B. daeguense and B. salitolerans were 51.7 ± 1.9%, 22.2 ± 4.0% and 52.4 ± 3.8%, respectively. Chemotaxonomic data (major sole isoprenoid quinone, MK-8(H₂); major diagnostic diamino acid, meso-diaminopimelic acid; major polyamines, putrescine and cadaverine; major cellular fatty acids, anteiso-C(15:0), iso-C(15:0) and anteiso-C(17:0); major polar lipids, phosphatidylglycerol and diphosphatidylglycerol; DNA G+C content, 69.3 mol%) also supported the affiliation of strain SJ5-8(T) to the genus Brevibacterium. Therefore, strain SJ5-8(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium jeotgali sp. nov. is proposed. The type strain is SJ5-8(T) ( =KACC 16911(T) =JCM 18571(T)).
authors.affiliation: School of Biological Sciences & Research Center for Biomolecules and Biosystems Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: School of Biological Sciences & Research Center for Biomolecules and Biosystems Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Se Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: School of Biological Sciences & Research Center for Biomolecules and Biosystems Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Jung
authors.affiliation: School of Biological Sciences & Research Center for Biomolecules and Biosystems Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D001482:Base Composition; D001951:Brevibacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017747:Seafood / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid