title: Mitochondrial DNA variability in populations of Centris aenea (Hymenoptera, Apidae), a crop-pollinating bee in Brazil.
abstract: Centris spp are oil-collecting solitary bees that are valuable pollinators of crops such as Brazil nut, cashew, and acerola. We investigated the genetic variability of populations of C. aenea in the northeastern region of Brazil. Total DNA was extracted from 59 individuals from 6 locations in the States of Pernambuco and Bahia and a 600-650-bp fragment of the mitochondrial COI/COII region amplified by PCR, followed by digestion with the restriction enzymes DraI and SspI. PCR-RFLP analysis revealed eight different haplotypes among the populations. Haplotype A1, revealed by DraI, was the most frequent (50%), and haplotypes A3 and A4 were exclusive to Feira de Santana, Bahia and Morro do Chapéu, Bahia, respectively. Among the haplotypes revealed by SspI, B2 was the most frequent (37%) and B3 was exclusive to Feira de Santana. This information revealing high haplotype diversity will be useful for developing management strategies for Centris, especially because of increasing interest in the rearing and/ or relocation of these bees for crop pollination.
authors.affiliation: Laboratório de Entomologia, Universidade Federal do Vale do São Francisco, Campus Ciências Agrárias, Petrolina, PE, Brasil. vininasf@gmail.com
authors.forename: V S
authors.initials: VS
authors.lastname: Ferreira
authors.forename: C M L
authors.initials: CM
authors.lastname: Aguiar
authors.forename: E J F
authors.initials: EJ
authors.lastname: Oliveira
authors.forename: M A
authors.initials: MA
authors.lastname: Costa
authors.forename: G M M
authors.initials: GM
authors.lastname: Santos
authors.forename: J G
authors.initials: JG
authors.lastname: Silva
mesh_terms: D000818:Animals; D001516:Bees / Q000145:classification / Q000235:genetics*; D001938:Brazil; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004272:DNA, Mitochondrial; D019476:Insect Proteins; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV; C061568:endodeoxyribonuclease SspI; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases