title: Lectin array analysis for wild-type and α-Gal-knockout pig islets versus healthy human islets.
abstract: OBJECTIVE
  We performed lectin microarray analyses of islets from wild-type (WT) pigs and α1-3galactosyltransferase gene knockout (GKO) pigs and compared the results with the corresponding values for islets from healthy humans.
  
  
  METHODS
  Islets were isolated from the pancreas. After sonication and centrifugation, the proteins in the supernatant from each islet were labeled with Cy3 and applied to a lectin array.
  
  
  RESULTS
  Despite negligible expression of the Gal antigen on the adult pig islets (APIs), GKO-islets showed weaker signals, not only for GS-I-B4 but also for PNA, WFA, PTL-I, and GS-I-A4, than the WT islets, indicating reduced contents of α-linked GalNAc and Galβ1-3GalNAc. In comparing the islets of pigs vs. humans, human islets showed stronger signals for UEA-I, AAL, TJA-II, EEL, WFA, HPA, DBA, SBA and PTL-I, indicating that besides ABO blood type antigens, high levels of fucose and α-linked GalNAc are present. On the other hand, the high mannose form was very rich in the APIs.
  
  
  CONCLUSIONS
  GKO reduced alpha-linked GalNAc, despite negligible expression of the Gal antigen on WT-API. On the other hand, the high-mannose form was richer in both APIs than in healthy human islets. These results provide useful information for future studies.
authors.affiliation: Division of Organ Transplantation, Department of Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan, miyagawa@orgtrp.med.osaka-u.ac.jp.
authors.forename: Shuji
authors.initials: S
authors.lastname: Miyagawa
authors.forename: Akira
authors.initials: A
authors.lastname: Maeda
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Takeishi
authors.forename: Takehisa
authors.initials: T
authors.lastname: Ueno
authors.forename: Noriaki
authors.initials: N
authors.lastname: Usui
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Teru
authors.initials: T
authors.lastname: Okitsu
authors.forename: Masafumi
authors.initials: M
authors.lastname: Goto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagashima
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis*; D000818:Animals; D000941:Antigens; D002478:Cells, Cultured; D004187:Disaccharides / Q000032:analysis*; D005685:Galactans / Q000032:analysis*; D005700:Galactosyltransferases / Q000235:genetics*; D055786:Gene Knockout Techniques; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology / Q000378:metabolism*; D037102:Lectins; D008358:Mannose; D011134:Polysaccharides / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D013552:Swine; D014183:Transplantation, Heterologous
chemical_list: D000941:Antigens; D004187:Disaccharides; D005685:Galactans; D037102:Lectins; D011134:Polysaccharides; C443716:galactosyll(1,3)-N-acetylgalactosamine; D005700:Galactosyltransferases; D000116:Acetylgalactosamine; D008358:Mannose
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