title: Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model.
abstract: A biodegradable fibrous tube was fabricated by electrospinning method using a combination of Poly(lactic-co-glycolic acid) (PLGA) and gelatin dissolved in trifluoroethanol (TFE). Different ratios of the two polymers (PLGA/Gelatin: 1/9, 3/7, 5/5) were used for electrospinning to determine the optimum condition appropriate for intestinal stent application. Fiber morphology was visualized and analyzed using a scanning electron microscope (SEM). Characterizations of physical properties were done according to its tensile strength, surface hydrophilicity, swelling ability, and biodegradability. Biocompatibility of the scaffolds was investigated in vitro using IEC-18 (Rat intestinal epithelial cell). Cell proliferation was quantified using MTT assay and cell adhesion behavior was visualized by SEM and confocal laser scanning microscope. PLGA/Gelatin (5/5) was determined to have adequate material properties and sufficient in vitro biocompatibility. This was then implanted in a male Sprague-Dawley rat for 14 days to determine in vivo behavior of the sample. Histological examination on the intestinal tissue surrounding the graft showed normal morphology comparable to non-implanted intestine.
authors.affiliation: Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Son
authors.forename: Rose-Ann
authors.initials: RA
authors.lastname: Franco
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Bae
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Min
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D020341:Absorbable Implants; D000714:Anastomosis, Surgical; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation; D005780:Gelatin / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000166:cytology; D007422:Intestines / Q000166:cytology / Q000601:surgery; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D015607:Stents
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005780:Gelatin
keywords: PLGA; biodegradable; electrospinning; gelatin; intestinal stent