title: Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs by using peptides selected from hypothetical proteins identified by an immunoproteomic approach.
abstract: In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruzi-infected animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected dogs were used. The study's findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of L. infantum infection in dogs.
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Chávez-Fumagalli
authors.forename: Vivian T
authors.initials: VT
authors.lastname: Martins
authors.forename: Miriam C S
authors.initials: MC
authors.lastname: Testasicca
authors.forename: Daniela P
authors.initials: DP
authors.lastname: Lage
authors.forename: Lourena E
authors.initials: LE
authors.lastname: Costa
authors.forename: Paula S
authors.initials: PS
authors.lastname: Lage
authors.forename: Mariana C
authors.initials: MC
authors.lastname: Duarte
authors.forename: Henrique G
authors.initials: HG
authors.lastname: Ker
authors.forename: Tatiana G
authors.initials: TG
authors.lastname: Ribeiro
authors.forename: Fernando A A
authors.initials: FA
authors.lastname: Carvalho
authors.forename: Wiliam C B
authors.initials: WC
authors.lastname: Régis
authors.forename: Alexandre B
authors.initials: AB
authors.lastname: Dos Reis
authors.forename: Carlos A P
authors.initials: CA
authors.lastname: Tavares
authors.forename: Manuel
authors.initials: M
authors.lastname: Soto
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Fernandes
authors.forename: Eduardo A F
authors.initials: EA
authors.lastname: Coelho
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001938:Brazil; D019295:Computational Biology / Q000379:methods; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D010455:Peptides; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000913:Antibodies, Protozoan; D010455:Peptides
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