title: Effect of an extract of Withania somnifera root on estrogen receptor-positive mammary carcinomas.
abstract: The chemopreventive activity of an extract of Withania somnifera (WS) roots was examined in female Sprague-Dawley rats that received the mammary carcinogen methylnitrosourea (MNU). The dose of the extract, administered by gavage, was 150 mg/kg body weight daily for 155 days, after injection of MNU. Rats in the treated group (N=15) had an average of 3.47 tumors, and rats in the control group (N=15) had 4.53, a reduction of 23%. The average weights of tumors were 4.98 g for rats in the treated group and 6.30 g for the controls, a difference of 21%. Labeling indices for Ki67 and proliferating cell nuclear antigen (PCNA) markers in cancers of the treated group were 42% and 38% lower, respectively, than those of the corresponding indices for the control group. These results indicate that the root extract significantly reduced the rate of cell division in the mammary tumors.
authors.affiliation: Department of Biomedical Sciences, School of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088, USA. kamel@mytu.tuskegee.edu
authors.forename: Kamel F
authors.initials: KF
authors.lastname: Khazal
authors.forename: Temesgen
authors.initials: T
authors.lastname: Samuel
authors.forename: Donald L
authors.initials: DL
authors.lastname: Hill
authors.forename: Clinton J
authors.initials: CJ
authors.lastname: Grubbs
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007124:Immunoenzyme Techniques; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008770:Methylnitrosourea / Q000633:toxicity; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000378:metabolism*; D032341:Withania / Q000737:chemistry*
chemical_list: D000477:Alkylating Agents; D010936:Plant Extracts; D011960:Receptors, Estrogen; D008770:Methylnitrosourea
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