title: Neopterin negatively regulates expression of ABCA1 and ABCG1 by the LXRα signaling pathway in THP-1 macrophage-derived foam cells.
abstract: To investigate the effects of neopterin on ABCA1 expression and cholesterol efflux in human THP-1 macrophage-derived foam cells, and to explore the role of the liver X receptor alpha (LXRα) involved. In the present study, THP-1 cells were pre-incubated with ox-LDL to become foam cells. The protein and mRNA expression were examined by Western blot assays and real-time quantitative PCR, respectively. Liquid scintillation counting and high performance liquid chromatography assays were used to test cellular cholesterol efflux and cholesterol content. Neopterin decreased ABCA1 expression and cholesterol efflux in a time- and concentration-dependent manner in THP-1 macrophage-derived foam cells, and the LXRα siRNA can reverse the inhibitory effects induced by neopterin. Neoterin has a negative regulation on ABCA1 expression via the LXRα signaling pathway, which suggests the aggravated effects of neopterin on atherosclerosis.
authors.affiliation: Affiliated Hospital of Shaoyang Medical College, Shaoyang, Hunan, China.
authors.forename: Jin-quan
authors.initials: JQ
authors.lastname: Yan
authors.forename: Chun-zhi
authors.initials: CZ
authors.lastname: Tan
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Wu
authors.forename: Dong-cui
authors.initials: DC
authors.lastname: Zhang
authors.forename: Ji-ling
authors.initials: JL
authors.lastname: Chen
authors.forename: Bin-yuan
authors.initials: BY
authors.lastname: Zeng
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Jiang
authors.forename: Jin
authors.initials: J
authors.lastname: Nie
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Dai
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics / Q000378:metabolism*; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000378:metabolism; D015536:Down-Regulation; D005487:Foam Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D006845:Hydrocarbons, Fluorinated / Q000494:pharmacology; D000071518:Liver X Receptors; D019798:Neopterin / Q000502:physiology*; D057093:Orphan Nuclear Receptors / Q000819:agonists / Q000378:metabolism*; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D005466:Fluorocarbons; D000096926:Benzenesulfonamides
chemical_list: D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D002784:Cholesterol; D006845:Hydrocarbons, Fluorinated; D000071518:Liver X Receptors; D019798:Neopterin; D057093:Orphan Nuclear Receptors; D013449:Sulfonamides; C577836:ABCA1 protein, human; C419353:ABCG1 protein, human; C000605213:NR1H3 protein, human; C423915:T0901317; D005466:Fluorocarbons; D000096926:Benzenesulfonamides
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