title: Harnessing single cell sorting to identify cell division genes and regulators in bacteria.
abstract: Cell division is an essential cellular process that requires an array of known and unknown proteins for its spatial and temporal regulation. Here we develop a novel, high-throughput screening method for the identification of bacterial cell division genes and regulators. The method combines the over-expression of a shotgun genomic expression library to perturb the cell division process with high-throughput flow cytometry sorting to screen many thousands of clones. Using this approach, we recovered clones with a filamentous morphology for the model bacterium, Escherichia coli. Genetic analysis revealed that our screen identified both known cell division genes, and genes that have not previously been identified to be involved in cell division. This novel screening strategy is applicable to a wide range of organisms, including pathogenic bacteria, where cell division genes and regulators are attractive drug targets for antibiotic development.
authors.affiliation: The ithree Institute, University of Technology, Sydney, New South Wales, Australia. Catherine.Burke@uts.edu.au
authors.forename: Catherine
authors.initials: C
authors.lastname: Burke
authors.forename: Michael
authors.initials: M
authors.lastname: Liu
authors.forename: Warwick
authors.initials: W
authors.lastname: Britton
authors.forename: James A
authors.initials: JA
authors.lastname: Triccas
authors.forename: Torsten
authors.initials: T
authors.lastname: Thomas
authors.forename: Adrian L
authors.initials: AL
authors.lastname: Smith
authors.forename: Steven
authors.initials: S
authors.lastname: Allen
authors.forename: Robert
authors.initials: R
authors.lastname: Salomon
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Harry
mesh_terms: D018407:Bacterial Physiological Phenomena / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics; D005434:Flow Cytometry; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology*
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