title: Interstitial engraftment of adipose-derived stem cells into an acellular dermal matrix results in improved inward angiogenesis and tissue incorporation.
abstract: Acellular dermal matrices (ADM) are commonly used in reconstructive procedures and rely on host cell invasion to become incorporated into host tissues. We investigated different approaches to adipose-derived stem cells (ASCs) engraftment into ADM to enhance this process. Lewis rat adipose-derived stem cells were isolated and grafted (3.0 × 10(5) cells) to porcine ADM disks (1.5 mm thick × 6 mm diameter) using either passive onlay or interstitial injection seeding techniques. Following incubation, seeding efficiency and seeded cell viability were measured in vitro. In addition, Eighteen Lewis rats underwent subcutaneous placement of ADM disk either as control or seeded with PKH67 labeled ASCs. ADM disks were seeded with ASCs using either onlay or injection techniques. On day 7 and or 14, ADM disks were harvested and analyzed for host cell infiltration. Onlay and injection techniques resulted in unique seeding patterns; however cell seeding efficiency and cell viability were similar. In-vivo studies showed significantly increased host cell infiltration towards the ASCs foci following injection seeding in comparison to control group (p < 0.05). Moreover, regional endothelial cell invasion was significantly greater in ASCs injected grafts in comparison to onlay seeding (p < 0.05). ADM can successfully be engrafted with ASCs. Interstitial engraftment of ASCs into ADM via injection enhances regional infiltration of host cells and angiogenesis, whereas onlay seeding showed relatively broad and superficial cell infiltration. These findings may be applied to improve the incorporation of avascular engineered constructs.
authors.affiliation: Division of Plastic, Reconstructive, Maxillofacial and Oral Surgery, Duke University Medical Center, Durham, North Carolina.
authors.forename: Issei
authors.initials: I
authors.lastname: Komatsu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: L Scott
authors.initials: LS
authors.lastname: Levin
authors.forename: Detlev
authors.initials: D
authors.lastname: Erdmann
authors.forename: Bruce
authors.initials: B
authors.lastname: Klitzman
authors.forename: Scott T
authors.initials: ST
authors.lastname: Hollenbeck
mesh_terms: D062088:Acellular Dermis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology; D008297:Male; D018919:Neovascularization, Physiologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D019919:Prosthesis Implantation; D051381:Rats; D011917:Rats, Inbred Lew; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D040521:Subcutaneous Tissue / Q000502:physiology*; D034421:Sus scrofa
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: acellular dermal matrix; adipose-derived stem cells; angiogenesis; cell engraftment