title: An investigation of the presence of specific anaerobic species in necrotic primary teeth.
abstract: Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The "red complex", which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, School of Dentistry, Univ Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil.
authors.forename: Genara Brum
authors.initials: GB
authors.lastname: Gomes
authors.forename: Rafael
authors.initials: R
authors.lastname: Sarkis-Onofre
authors.forename: Maria Laura Menezes
authors.initials: ML
authors.lastname: Bonow
authors.forename: Adriana
authors.initials: A
authors.lastname: Etges
authors.forename: Rogério Castilho
authors.initials: RC
authors.lastname: Jacinto
mesh_terms: D001421:Bacteria, Anaerobic / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014094:Tooth, Deciduous / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial