title: Autoantigenicity of carbonic anhydrase 1 in patients with abdominal aortic aneurysm, revealed by proteomic surveillance.
abstract: Abdominal aortic aneurysm (AAA) is sometimes detected in patients with atherosclerosis. One of the histological characteristics of AAA walls is infiltration of inflammatory cells, in which autoimmunity may be involved. Thereby, we here surveyed autoantigens in AAA walls by proteomics. Specifically, we separated proteins extracted from AAA wall samples by 2-dimensional electrophoresis and detected candidate autoantigens by western blotting. One of the detected candidates was carbonic anhydrase 1 (CA1). ELISA confirmed that the autoantibodies to CA1 were detected more frequently in AAA patients (n=13) than in healthy donors (n=25) (p=0.03). Interestingly, some serum samples from the AAA patients reacted to CA1 of the AAA walls stronger than to CA1 of peripheral blood mononuclear cells from healthy donors. Our data indicate that CA1 in the AAA walls would be modified to express neo-epitope(s) and that the autoimmunity to CA1 may be involved in the pathogenesis of AAA.
authors.affiliation: Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kanagawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ando
authors.forename: Nobuko
authors.initials: N
authors.lastname: Iizuka
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sato
authors.forename: Masahide
authors.initials: M
authors.lastname: Chikada
authors.forename: Manae S
authors.initials: MS
authors.lastname: Kurokawa
authors.forename: Mitsumi
authors.initials: M
authors.lastname: Arito
authors.forename: Kazuki
authors.initials: K
authors.lastname: Okamoto
authors.forename: Naoya
authors.initials: N
authors.lastname: Suematsu
authors.forename: Haruo
authors.initials: H
authors.lastname: Makuuchi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kato
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017544:Aortic Aneurysm, Abdominal / Q000175:diagnosis / Q000276:immunology*; D050197:Atherosclerosis / Q000175:diagnosis / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001808:Blood Vessels / Q000276:immunology; D024401:Carbonic Anhydrase I / Q000097:blood / Q000276:immunology*; D002457:Cell Extracts; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D020543:Proteome
chemical_list: D001324:Autoantigens; D002457:Cell Extracts; D020543:Proteome; D024401:Carbonic Anhydrase I