title: ESCRT-III assembly and cytokinetic abscission are induced by tension release in the intercellular bridge.
abstract: The last step of cell division, cytokinesis, produces two daughter cells that remain connected by an intercellular bridge. This state often represents the longest stage of the division process. Severing the bridge (abscission) requires a well-described series of molecular events, but the trigger for abscission remains unknown. We found that pulling forces exerted by daughter cells on the intercellular bridge appear to regulate abscission. Counterintuitively, these forces prolonged connection, whereas a release of tension induced abscission. Tension release triggered the assembly of ESCRT-III (endosomal sorting complex required for transport-III), which was followed by membrane fission. This mechanism may allow daughter cells to remain connected until they have settled in their final locations, a process potentially important for tissue organization and morphogenesis.
authors.affiliation: Institut Curie, CNRS UMR 144, Paris, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Lafaurie-Janvore
authors.forename: Paolo
authors.initials: P
authors.lastname: Maiuri
authors.forename: Irène
authors.initials: I
authors.lastname: Wang
authors.forename: Mathieu
authors.initials: M
authors.lastname: Pinot
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Manneville
authors.forename: Timo
authors.initials: T
authors.lastname: Betz
authors.forename: Martial
authors.initials: M
authors.lastname: Balland
authors.forename: Matthieu
authors.initials: M
authors.lastname: Piel
mesh_terms: D002450:Cell Communication; D048749:Cytokinesis; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D055595:Mechanical Phenomena; D034741:RNA, Small Interfering / Q000235:genetics; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: C507674:CHMP2A protein, human; D056827:Endosomal Sorting Complexes Required for Transport; D034741:RNA, Small Interfering; D014404:Tubulin; D049452:Green Fluorescent Proteins