title: Toward improving fine needle aspiration cytology by applying Raman microspectroscopy.
abstract: ABSTRACT. Medical diagnosis of biopsies performed by fine needle aspiration has to be very reliable. Therefore, pathologists/cytologists need additional biochemical information on single cancer cells for an accurate diagnosis. Accordingly, we applied three different classification models for discriminating various features of six breast cancer cell lines by analyzing Raman microspectroscopic data. The statistical evaluations are implemented by linear discriminant analysis (LDA) and support vector machines (SVM). For the first model, a total of 61,580 Raman spectra from 110 single cells are discriminated at the cell-line level with an accuracy of 99.52% using an SVM. The LDA classification based on Raman data achieved an accuracy of 94.04% by discriminating cell lines by their origin (solid tumor versus pleural effusion). In the third model, Raman cell spectra are classified by their cancer subtypes. LDA results show an accuracy of 97.45% and specificities of 97.78%, 99.11%, and 98.97% for the subtypes basal-like, HER2+/ER-, and luminal, respectively. These subtypes are confirmed by gene expression patterns, which are important prognostic features in diagnosis. This work shows the applicability of Raman spectroscopy and statistical data handling in analyzing cancer-relevant biochemical information for advanced medical diagnosis on the single-cell level.
authors.affiliation: University of Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Becker-Putsche
authors.forename: Thomas
authors.initials: T
authors.lastname: Bocklitz
authors.forename: Joachim
authors.initials: J
authors.lastname: Clement
authors.forename: Petra
authors.initials: P
authors.lastname: Rösch
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D044963:Biopsy, Fine-Needle / Q000379:methods*; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology; D045744:Cell Line, Tumor; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D013059:Spectrum Analysis, Raman / Q000379:methods*; D060388:Support Vector Machine