title: A transdermal delivery system to enhance quercetin nanoparticle permeability.
abstract: Targeting oxidative stress with inhibiting or boosting the endogenous levels of antioxidants potentially has an outstanding effect in the treatment of oxidative-stress-related diseases. The present work demonstrates the synthesis of quercetin nanoemulsion as one of the potential antioxidants for the treatment of oxidative- stress-related diseases. A quercetin nanoemulsion was prepared using poly(lactic-co-glycolic acid) (PLGA), hyaluronic acid (HA) and emulsifier (Tween-20) through a solvent evaporation technique. The efficiency of the nanoemulsion was evaluated with and without chemical permeation enhancer (CPE). The FT-IR result shows no interaction between quercetin and polymer that proves excellent compatibility. The transdermal delivery ability was evaluated using in vitro release and ex vivo permeation analysis. The transdermal drug-release mechanism was studied by the mathematical model and was found to obey a zero-order, diffusion-controlled mechanism. In vitro toxicity and cell behavior, including cell adhesion, proliferation and cell death of quercetin-nanoemulsion-treated L929 cells, were elucidated by the electrical cell-substrate impedance sensing (ECIS) technique. The produced nanoemulsion showed a high encapsulation efficiency, less toxicity, controlled delivery with enhanced transdermal drug permeation and effective scavenging of free radicals.
authors.affiliation: Department of Bionanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do, 461-701, South Korea.
authors.forename: Devasier
authors.initials: D
authors.lastname: Bennet
authors.forename: Sanghyo
authors.initials: S
authors.lastname: Kim
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000633:toxicity; D019344:Lactic Acid / Q000737:chemistry* / Q000633:toxicity; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D019301:Oleic Acid / Q000737:chemistry / Q000633:toxicity; D010539:Permeability; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000633:toxicity; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011794:Quercetin / Q000008:administration & dosage* / Q000494:pharmacology; D012867:Skin / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D004655:Emulsions; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019301:Oleic Acid; D019344:Lactic Acid; D006820:Hyaluronic Acid; D011794:Quercetin