title: Functional analysis-make or break for cancer predictability.
abstract: Copy number variations (CNVs) encompass a variety of genetic alterations including deletions and amplifications and cluster in regions of the human genome with intrinsic instability. Small-sized CNVs can act as initial genetic changes giving rise to larger CNVs such as acquired somatic copy number aberrations (CNAs) promoting cancer formation. Previous studies provided evidence for CNVs as an underlying cause of elevated breast cancer risk when targeting breast cancer susceptibility genes and of accelerated breast cancer progression when targeting oncogenes. With the development of novel techniques for genome-wide detection of CNVs at increasingly higher resolution, it became possible to qualitatively and quantitatively analyse manifestation of DNA damage resulting from defects in any of the large variety of DNA double-strand break (DSB) repair mechanisms. Breast carcinogenesis, particularly in familial cases, has been linked with a defect in the homologous recombination (HR) pathway, which in turn switches damage removal towards alternative, more error-prone DSB repair pathways such as microhomology-mediated non-homologous end joining (mmNHEJ). Indeed, increased error-prone DSB repair activities were detected in peripheral blood lymphocytes from individuals with familial breast cancer risk independently of specific gene mutations. Intriguingly, sequence analysis of breakpoint regions revealed that the majority of genome aberrations found in breast cancer specimens are formed by mmNHEJ. Detection of pathway-specific error-prone DSB repair activities by functional testing was proposed to serve as biomarker for hereditary breast cancer risk and responsiveness to therapies targeting HR dysfunction. Identification of specific error-prone DSB repair mechanisms underlying CNAs and ultimately mammary tumour formation highlights potential targets for future breast cancer prevention regimens.
authors.affiliation: Department of Obstetrics and Gynaecology, Ulm University, Germany. Electronic address: miriam_deniz@web.de.
authors.forename: Miriam
authors.initials: M
authors.lastname: Deniz
authors.affiliation: Chip-Facility, IZKF, Ulm University, Germany. Electronic address: karlheinz.holzmann@uni-ulm.de.
authors.forename: Karlheinz
authors.initials: K
authors.lastname: Holzmann
authors.affiliation: Department of Obstetrics and Gynaecology, Ulm University, Germany. Electronic address: lisa.wiesmueller@uni-ulm.de.
authors.forename: Lisa
authors.initials: L
authors.lastname: Wiesmüller
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002869:Chromosome Aberrations; D053903:DNA Breaks, Double-Stranded; D056915:DNA Copy Number Variations; D004260:DNA Repair; D005260:Female; D020022:Genetic Predisposition to Disease; D015894:Genome, Human; D042822:Genomic Instability; D006801:Humans