title: Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells.
abstract: In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, India.
authors.forename: B
authors.initials: B
authors.lastname: Swarnalatha
authors.forename: S L
authors.initials: SL
authors.lastname: Nair
authors.forename: K T
authors.initials: KT
authors.lastname: Shalumon
authors.forename: L C
authors.initials: LC
authors.lastname: Milbauer
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
authors.forename: B
authors.initials: B
authors.lastname: Paul-Prasanth
authors.forename: K K
authors.initials: KK
authors.lastname: Menon
authors.forename: R P
authors.initials: RP
authors.lastname: Hebbel
authors.forename: A
authors.initials: A
authors.lastname: Somani
authors.forename: Shantikumar V
authors.initials: SV
authors.lastname: Nair
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000502:physiology; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D003470:Culture Media; D042783:Endothelial Cells / Q000502:physiology*; D019344:Lactic Acid / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D044968:Regenerative Medicine; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D003470:Culture Media; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D048271:Chitosan; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat