title: Extracellular polymeric substances from two biofilm forming Vibrio species: characterization and applications.
abstract: The early biofilm colonizing Vibrio campbellii and Vibrio fortis produced 400 and 134 mg L(-1) extracellular polymeric substances (EPS), respectively of distinct composition in planktonic cultures. The EPS of V. campbellii consisted of five monosaccharides: arabinose, galactose, glucose, mannose and rhamnose, however, that of V. fortis has only three monosaccharides: arabinose, galactose and mannose. The AFM topography analysis showed different morphology and physical characteristics of EPSs. FTIR and NMR spectral analyses revealed characteristic functional groups of the polysaccharides. EPSs were amorphous in nature and contained characteristic diffraction peaks. EPSs showed high emulsifying activity and pseudoplastic rheology. This is the first report of detailed characterization of EPS produced by V. campbellii and V. fortis, so far. Detailed analytical (FT-IR, (1)H NMR, PXRD, etc.) and bio-physicochemical characteristics (thermostability, emulsifying and rheological properties) of EPSs showed potential for industrial applications.
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G.B. Marg, Bhavnagar 364 002, Gujarat, India.
authors.forename: Kumari
authors.initials: K
authors.lastname: Kavita
authors.forename: Avinash
authors.initials: A
authors.lastname: Mishra
authors.forename: Bhavanath
authors.initials: B
authors.lastname: Jha
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012212:Rheology; D013499:Surface Properties; D014733:Vibrio / Q000235:genetics / Q000502:physiology*
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S