title: Profiling the gastrointestinal microbiota in response to Salmonella: low versus high Salmonella shedding in the natural porcine host.
abstract: Controlling Salmonella in the food chain is complicated by the ability of Salmonella to colonize livestock without causing clinical symptoms/disease. Salmonella-carrier animals are a significant reservoir for contamination of naïve animals, the environment, and our food supply. Salmonella carriage and shedding in pigs varies greatly both experimentally and on-farm. To investigate the dynamics between the porcine intestinal microbiota and Salmonella shedding, we temporally profiled the microbiota of pigs retrospectively classified as low and high Salmonella-shedders. Fifty-four piglets were collectively housed, fed and challenged with 10(9)Salmonella enterica serovar Typhimurium. Bacterial quantitation of Salmonella in swine feces was determined, and total fecal DNA was isolated for 16S rRNA gene sequencing from groups of high-shedder, low-shedder, and non-inoculated pigs (n=5/group; 15 pigs total). Analyses of bacterial community structures revealed significant differences between the microbiota of high-shedder and low-shedder pigs before inoculation and at 2 and 7 days post-inoculation (d.p.i.); microbiota differences were not detected between low-shedder and non-inoculated pigs. Because the microbiota composition prior to Salmonella challenge may influence future shedding status, the "will-be" high and low shedder phylotypes were compared, revealing higher abundance of the Ruminococcaceae family in the "will-be" low shedders. At 2d.p.i., a significant difference in evenness for the high shedder microbiota compared to the other two groups was driven by decreases in Prevotella abundance and increases in various genera (e.g. Catenibacterium, Xylanibacter). By 21 d.p.i., the microbial communities of high-shedder and low-shedder pigs were no longer significantly different from one another, but were both significantly different from non-inoculated pigs, suggesting a similar Salmonella-induced alteration in maturation of the swine intestinal microbiota regardless of shedding status. Our results correlate microbial shifts with Salmonella shedding status in pigs, further defining the complex interactions among the host, pathogen, and microbiota of this important public health issue and food safety concern.
authors.affiliation: USDA/ARS/National Animal Disease Center, Ames, IA 50010, USA. shawn.bearson@ars.usda.gov
authors.forename: Shawn M D
authors.initials: SM
authors.lastname: Bearson
authors.forename: Heather K
authors.initials: HK
authors.lastname: Allen
authors.forename: Bradley L
authors.initials: BL
authors.lastname: Bearson
authors.forename: Torey
authors.initials: T
authors.lastname: Looft
authors.forename: Brian W
authors.initials: BW
authors.lastname: Brunelle
authors.forename: Jalusa D
authors.initials: JD
authors.lastname: Kich
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Tuggle
authors.forename: Darrell O
authors.initials: DO
authors.lastname: Bayles
authors.forename: David
authors.initials: D
authors.lastname: Alt
authors.forename: Uri Y
authors.initials: UY
authors.lastname: Levine
authors.forename: Thaddeus B
authors.initials: TB
authors.lastname: Stanton
mesh_terms: D000818:Animals; D057138:Bacterial Shedding; D001831:Body Temperature; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology; D054884:Host-Pathogen Interactions; D064307:Microbiota / Q000502:physiology*; D018720:Prevotella / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D013552:Swine
chemical_list: D004269:DNA, Bacterial