title: Beneficial influence of topical extra virgin olive oil application on an experimental model of penile fracture in rats.
abstract: Penile fracture (PF) is known as a traumatic rupture of the tunica albuginea of corpus cavernosum. In this study, we aimed to investigate the healing influence of topical extra virgin olive oil (EVOO) on PF through evaluating levels of some oxidative stress biomarkers for the first time. Histopathological evaluation was also realized. A total of 18 male Sprague-Dawley albino rats were divided into three groups of six rats each as control group, in PF (alone) group, and PF + EVOO group. Experimental PF was formed via incising from the proximal dorsal side of the penis in the rats of all groups except control. While in PF (alone) group, fracture was formed and the incision was primarily closed, in PF + EVOO group in addition to foregoing processes, EVOO was also administrated topically twice a day for 3 weeks. At the end of the experiment, all rats were killed and penectomy was carried out. While malondialdehyde, myeloperoxidase, lipid hyroperoxide, and total oxidant status significantly (p < 0.05) increased, reduced glutathione and total free sulfhydryl groups markedly (p < 0.05) decreased in PF (alone) group when compared with PF + EVOO group. Levels of these parameters were reversed to nearly normal values by topical EVOO application. Protection by EVOO is further substantiated via the improved histological findings in PF + EVOO group as against degenerative changes in the rats of PF (alone) group. Our data revealed that EVOO has protective effect in penile cavernosal tissue through probably its antioxidant, free radical defusing, anti-inflammatory, and antimicrobial effects.
authors.affiliation: Department of Urology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Gunes
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey h_ozkol@mynet.com.
authors.forename: Halil
authors.initials: H
authors.lastname: Ozkol
authors.affiliation: Department of Urology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Necip
authors.initials: N
authors.lastname: Pirincci
authors.affiliation: Department of Urology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Ilhan
authors.initials: I
authors.lastname: Gecit
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Salim
authors.initials: S
authors.lastname: Bilici
authors.affiliation: Department of Pathology, Faculty of Veterinary, Yuzuncu Yil University, Van, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Yildirim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers; D008297:Male; D000069463:Olive Oil / Q000008:administration & dosage / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects*; D010409:Penile Diseases / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D000069463:Olive Oil
keywords: Extra virgin olive oil; antioxidant; free radical; histopathological; penile fracture; rat