title: The effect of high-intensity focused ultrasound treatment on immune function in patients with uterine fibroids.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of high-intensity focused ultrasound (HIFU) on immune function in patients with uterine fibroids, in a randomised comparison to conventional myomectomy.
  
  
  METHODS
  The patients were assigned (1:1) to the HIFU group or the myomectomy (MY) group. Venous blood samples were collected 24 h before and 24 h and 72 h after operation. The percentages of CD4(+) and CD8(+) T cells and natural killer (NK) cells were quantified by flow cytometry (FCM). Serum levels of interleukin-2 (IL-2), IL-6 and IL-10 were determined using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  HIFU was associated with early ambulation, fewer post-operative complications, and shorter hospital stay (p < 0.001). The percentages of CD4(+) and CD8(+) T cells and NK cells in the HIFU group were not significantly altered after treatment compared with before treatment. In contrast, the numbers of these cells in the MY group decreased significantly 24 h after conventional myomectomy (p < 0.001). The CD4(+)/CD8(+) T cell ratios were also decreased significantly 24 h and 72 h after conventional myomectomy (p < 0.001). Serum levels of IL-6 and IL-10 increased after treatment in both groups. Peak IL-6 and IL-10 levels were significantly lower in the HIFU group than in the MY group (p < 0.001). In contrast, IL-2 level decreased significantly in the MY group compared to the HIFU group at 24 h post-operation (p < 0.001).
  
  
  CONCLUSIONS
  Short-term post-operative immune function is better preserved after HIFU treatment. Better preserved immune function may reflect a reduction in tissue trauma after HIFU treatment and contribute to reduced post-operative complications.
authors.affiliation: Department of Obstetrics and Gynaecology, First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Qin
authors.forename: Jinyun
authors.initials: J
authors.lastname: Chen
authors.forename: Lifang
authors.initials: L
authors.lastname: Wang
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Chen
authors.forename: Liangdan
authors.initials: L
authors.lastname: Tang
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000097:blood; D005260:Female; D057086:High-Intensity Focused Ultrasound Ablation / Q000009:adverse effects; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D007889:Leiomyoma / Q000097:blood / Q000276:immunology*; D018655:Lymphocyte Count; D063186:Uterine Myomectomy / Q000009:adverse effects
chemical_list: D016207:Cytokines