title: Supplementation with α-lipoic acid, CoQ10, and vitamin E augments running performance and mitochondrial function in female mice.
abstract: Antioxidant supplements are widely consumed by the general public; however, their effects of on exercise performance are controversial. The aim of this study was to examine the effects of an antioxidant cocktail (α-lipoic acid, vitamin E and coenzyme Q10) on exercise performance, muscle function and training adaptations in mice. C57Bl/J6 mice were placed on antioxidant supplement or placebo-control diets (n = 36/group) and divided into trained (8 wks treadmill running) (n = 12/group) and untrained groups (n = 24/group). Antioxidant supplementation had no effect on the running performance of trained mice nor did it affect training adaptations; however, untrained female mice that received antioxidants performed significantly better than placebo-control mice (p ≤ 0.05). Furthermore, antioxidant-supplemented females (untrained) showed elevated respiratory capacity in freshly excised muscle fibers (quadriceps femoris) (p ≤ 0.05), reduced oxidative damage to muscle proteins (p ≤ 0.05), and increased expression of mitochondrial proteins (p ≤ 0.05) compared to placebo-controls. These changes were attributed to increased expression of proliferator-activated receptor gamma coactivator 1α (PGC-1α) (p ≤ 0.05) via activation of AMP-activated protein kinase (AMPK) (p ≤ 0.05) by antioxidant supplementation. Overall, these results indicate that this antioxidant supplement exerts gender specific effects; augmenting performance and mitochondrial function in untrained females, but does not attenuate training adaptations.
authors.affiliation: Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Arkan
authors.initials: A
authors.lastname: Abadi
authors.forename: Justin D
authors.initials: JD
authors.lastname: Crane
authors.forename: Daniel
authors.initials: D
authors.lastname: Ogborn
authors.forename: Bart
authors.initials: B
authors.lastname: Hettinga
authors.forename: Mahmood
authors.initials: M
authors.lastname: Akhtar
authors.forename: Andrew
authors.initials: A
authors.lastname: Stokl
authors.forename: Lauren
authors.initials: L
authors.lastname: Macneil
authors.forename: Adeel
authors.initials: A
authors.lastname: Safdar
authors.forename: Mark
authors.initials: M
authors.lastname: Tarnopolsky
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019587:Dietary Supplements; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects; D005082:Physical Exertion / Q000187:drug effects; D008063:Thioctic Acid / Q000494:pharmacology*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014451:Ubiquinone; D014810:Vitamin E; D008063:Thioctic Acid; C024989:coenzyme Q10
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