title: [MicroRNA differential expression profiles in DC after the stimulation of HBsAg].
abstract: OBJECTIVE
  To establish the differential expression profiles for exploring new immune mechanism of hepatitis B virus infection.
  
  
  METHODS
  DCs were separated from the bone marrow of healthy mouse and cultured in vitro. Then DCs were stimulated with HBsAg, LPS, TNF-alpha, respectively. Twenty-four hours later, the total RNA of cell was extracted. cDNA microarray was used to compare the differential expression of RNA. The significant differential expression of miRNA after the stimulation of HBsAg was chosen. Subsequently, target genes of the significant differential miRNA were forecasted by using computer software.
  
  
  RESULTS
  There were 30 miRNAs whose significant differential expressions were beyond two times after the stimulation of HBsAg. Among them, 16 miRNAs expressions were increased and 14 miRNAs expressions were decreased. There was significant difference in differential expression of miRNA among the 3 different stimulations. The selected target genes included relevant elements of signal pathway of DC.
  
  
  CONCLUSIONS
  The alteration of expression profiles of miRNA was specific after DC was stimulated by HBsAg. The selected target genes further demonstrated that miRNA could play important roles in the immune mechanism of HBV infection.
authors.affiliation: Department of Infectious Diseases, Peking University Shenzhen Hospital, Shenzhen 518036, China.
authors.forename: Xiao-Jia
authors.initials: XJ
authors.lastname: Xiong
authors.forename: Yan-Zhong
authors.initials: YZ
authors.lastname: Peng
authors.forename: Xing-Fei
authors.initials: XF
authors.lastname: Pan
authors.forename: Qi-Huan
authors.initials: QH
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism*; D020869:Gene Expression Profiling; D006514:Hepatitis B Surface Antigens / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D006514:Hepatitis B Surface Antigens; D008070:Lipopolysaccharides; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha