title: Effect of overexpression of the brain-specific Na(+)/Ca(2+) exchanger splice variant NCX1.5 on NO cytotoxicity in HEK293 cells.
abstract: Nitric oxide (NO) induces cytotoxicity in neuronal and glial cells via activation of the Na(+)/Ca(2+) exchanger (NCX). This study examined the role of the predominant brain-specific NCX splice variant NCX1.5 in NO-induced cytotoxicity in the HEK293 cell expression system. Cells were transfected with the plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA. There was no difference in the cytotoxic effects of the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine between control and transfected cells. These results suggest that NO cytotoxicity is not dependent on NCX1.5.
authors.affiliation: Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ota
authors.forename: Takuya
authors.initials: T
authors.lastname: Kawanai
authors.forename: Ryo
authors.initials: R
authors.lastname: Watanabe
authors.forename: Akira
authors.initials: A
authors.lastname: Nishimura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ago
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takuma
authors.forename: Toshio
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects* / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression / Q000235:genetics* / Q000502:physiology*; D014644:Genetic Variation / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000633:toxicity*; D020030:Nitric Oxide Donors / Q000633:toxicity; D019154:Protein Splicing / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D019831:Sodium-Calcium Exchanger / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology
chemical_list: D020030:Nitric Oxide Donors; D019831:Sodium-Calcium Exchanger; C110374:sodium-calcium exchanger 1; D009569:Nitric Oxide