title: Comparison of polystyrene nanoparticles and UV-inactivated antigen-displaying adenovirus for vaccine delivery in mice.
abstract: BACKGROUND
  Inert nanoparticles are attracting attention as carriers for protein-based vaccines. Here we evaluate the immunogenicity of the model antigen ovalbumin delivered on polystyrene particles and directly compare particulate delivery with adenovirus-based immunization.
  
  
  RESULTS
  Mice were vaccinated with soluble ovalbumin, ovalbumin-coated polystyrene particles of different sizes, or an adenovirus-based expression-display vector that encodes and displays a pIX-ovalbumin fusion protein. Antibody responses were clearly higher when ovalbumin was administered on polystyrene particles compared to soluble protein administration, regardless of the particle size. Compared to adenovirus-based immunization, antibody levels were lower if an equivalent amount of protein was delivered, and no cellular immune response was detectable.
  
  
  CONCLUSIONS
  We demonstrate in a side-by-side comparison that inert nanoparticles allow for the reduction of the administered antigen amount compared to immunization with soluble protein and induce strongly enhanced antibody responses, but responses are lower compared to adenovirus-based immunization.
authors.affiliation: Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Johrden
authors.forename: Matthias
authors.initials: M
authors.lastname: Tenbusch
authors.forename: Ruth
authors.initials: R
authors.lastname: Lietz
authors.forename: Michael Storcksdieck Genannt
authors.initials: MS
authors.lastname: Bonsmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Niezold
authors.forename: Oliver
authors.initials: O
authors.lastname: Wildner
authors.forename: Wibke
authors.initials: W
authors.lastname: Bayer
mesh_terms: D000256:Adenoviridae / Q000737:chemistry*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011137:Polystyrenes; D014611:Vaccination / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000941:Antigens; D004337:Drug Carriers; D011137:Polystyrenes; D014612:Vaccines; D010047:Ovalbumin
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