title: Blood-pressure-lowering effect of glycosaminoglycan derived from Isaria sinclairii in spontaneously hypertensive rats.
abstract: The antihypertensive effects of both extracts and glycosaminoglycan derived from Isaria sinclairii (IS) were investigated in a spontaneously hypertensive rat (SHR) model. Groups of rats were treated orally with 30 mg/kg each of: (1) saline control or extracts of (2) water-IS (3) methanol-IS, (4) butanol-IS, (5) ethyl acetate-IS, or (6) captopril as positive control. The 30-mg/kg dose was administered with a standard diet every day for a period of 2 wk. The antihypertensive effects of the individual extracts were in the following order: methanol > water > ethyl acetate > butanol. Glycosaminoglycan (GAG) obtained from IS as a water-soluble alcohol precipitation fraction produced an antihypertensive effect. One month following administration of GAG derived from IS to SHR animals there was a marked decrease in systolic blood pressure from 183 to 105 mm Hg and reduced diastolic blood pressure from 148 to 80 mm Hg compared to untreated control SHR rats. It was found that GAG produced an antihypertensive effect, which was more effective than the positive control captopril. In the SHR animal model a fall of 19% in body weight was observed in the group that received GAG. Data thus indicate that GAG derived from I. sinclairii may be a potent, naturally occurring antihypertensive agent.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon, South Korea. amy@korea.kr
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Yi Sook
authors.initials: YS
authors.lastname: Jung
authors.forename: Sang Duck
authors.initials: SD
authors.lastname: Jee
authors.forename: Jea Woong
authors.initials: JW
authors.lastname: Han
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Byung Mu
authors.initials: BM
authors.lastname: Lee
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D006999:Hypocreales / Q000737:chemistry*; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D015430:Weight Gain / Q000187:drug effects; D014914:Whole Blood Coagulation Time
chemical_list: D000959:Antihypertensive Agents; D006025:Glycosaminoglycans; D010936:Plant Extracts; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III