title: High fat and high cholesterol diet induces DPP-IV activity in intestinal lymph.
abstract: Recent studies have reported that dipeptidyl-peptitase IV (DPP-IV) is correlated with diabetic conditions and also with dyslipidemia caused by overnutrition, especially a high fat diet. However, the role of DPP-IV in diabetes during dyslipidemia has been unclear. We utilized a lymph fistula rat model to determine whether intestinal lymph, which absorbs dietary fats, is affected by a chronic high-fat and high-cholesterol diet (HFHC). HFHC diet rats showed significantly higher DPP-IV activity in intestinal lymph and plasma compared to rats receiving a normal chow diet. In addition, HFHC diet rats showed significantly increased DPP-IV mRNA expression in the intestine. However, DPP-IV mRNA in the lymphocytes isolated from intestinal lymph and mesenteric lymph nodes did not show significant differences from that in the normal diet rats. In conclusion, HFHC diets increased DPP-IV expression in intestinal lymph; these results indicate the applicability of a previously unrecognized role for DPP-IV in metabolic disorders, including diabetes.
authors.affiliation: Institute of Environmental Science for Human life, Ochanomizu University, Tokyo, Japan.
authors.forename: Miku
authors.initials: M
authors.lastname: Toyozaki
authors.forename: Mizuko
authors.initials: M
authors.lastname: Osaka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kondo
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D059305:Diet, High-Fat; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D007422:Intestines / Q000187:drug effects / Q000201:enzymology*; D008196:Lymph / Q000187:drug effects / Q000201:enzymology*; D008198:Lymph Nodes / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002791:Cholesterol, Dietary; D012333:RNA, Messenger; D018819:Dipeptidyl Peptidase 4