title: The contribution of ArsB to arsenic resistance in Campylobacter jejuni.
abstract: Arsenic, a toxic metalloid, exists in the natural environment and its organic form is approved for use as a feed additive for animal production. As a major foodborne pathogen of animal origin, Campylobacter is exposed to arsenic selection pressure in the food animal production environments. Previous studies showed that Campylobacter isolates from poultry were highly resistant to arsenic compounds and a 4-gene operon (containing arsP, arsR, arsC, and acr3) was associated with arsenic resistance in Campylobacter. However, this 4-gene operon is only present in some Campylobacter isolates and other arsenic resistance mechanisms in C. jejuni have not been characterized. In this study, we determined the role of several putative arsenic resistance genes including arsB, arsC2, and arsR3 in arsenic resistance in C. jejuni and found that arsB, but not the other two genes, contributes to the resistance to arsenite and arsenate. Inactivation of arsB in C. jejuni resulted in 8- and 4-fold reduction in the MICs of arsenite and arsenate, respectively, and complementation of the arsB mutant restored the MIC of arsenite. Additionally, overexpression of arsB in C. jejuni 11168 resulted in a 16-fold increase in the MIC of arsenite. PCR analysis of C. jejuni isolates from different animals hosts indicated that arsB and acr3 (the 4-gene operon) are widely distributed in various C. jejuni strains, suggesting that Campylobacter requires at least one of the two genes for adaptation to arsenic-containing environments. These results identify ArsB as an alternative mechanism for arsenic resistance in C. jejuni and provide new insights into the adaptive mechanisms of Campylobacter in animal food production environments.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Zhangqi
authors.initials: Z
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Orhan
authors.initials: O
authors.lastname: Sahin
authors.forename: Qijing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D001152:Arsenicals / Q000494:pharmacology*; D018053:Arsenites / Q000494:pharmacology; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D023061:Gene Order; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009876:Operon
chemical_list: D001152:Arsenicals; D018053:Arsenites; D008565:Membrane Proteins; D009097:Multienzyme Complexes
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