title: Ancient host shifts followed by host conservatism in a group of ant parasitoids.
abstract: While ant colonies serve as host to a diverse array of myrmecophiles, few parasitoids are able to exploit this vast resource. A notable exception is the wasp family Eucharitidae, which is the only family of insects known to exclusively parasitize ants. Worldwide, approximately 700 Eucharitidae species attack five subfamilies across the ant phylogeny. Our goal is to uncover the pattern of eucharitid diversification, including timing of key evolutionary events, biogeographic patterns and potential cophylogeny with ant hosts. We present the most comprehensive molecular phylogeny of Eucharitidae to date, including 44 of the 53 genera and fossil-calibrated estimates of divergence dates. Eucharitidae arose approximately 50 Ma after their hosts, during the time when the major ant lineages were already established and diversifying. We incorporate host association data to test for congruence between eucharitid and ant phylogenies and find that their evolutionary histories are more similar than expected at random. After a series of initial host shifts, clades within Eucharitidae maintained their host affinity. Even after multiple dispersal events to the New World and extensive speciation within biogeographic regions, eucharitids remain parasitic on the same ant subfamilies as their Old World relatives, suggesting host conservatism despite access to a diverse novel ant fauna.
authors.affiliation: Department of Entomology, University of California, Riverside, CA 92521, USA. elizabeth.murray@email.ucr.edu
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Murray
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Carmichael
authors.forename: John M
authors.initials: JM
authors.lastname: Heraty
mesh_terms: D000818:Animals; D001000:Ants / Q000469:parasitology*; D005075:Biological Evolution; D002467:Cell Nucleus / Q000235:genetics; D019143:Evolution, Molecular; D006790:Host-Parasite Interactions; D024101:Mitochondrial Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D014863:Wasps / Q000166:cytology / Q000235:genetics / Q000502:physiology*
chemical_list: D024101:Mitochondrial Proteins
references.citation: Evolution. 2007 Jun;61(6):1368-79
references.pmid: 17542846
references.citation: Algorithms Mol Biol. 2010 Feb 03;5:16
references.pmid: 20181081
references.citation: Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18172-7
references.pmid: 17079492
references.citation: Syst Biol. 2004 Oct;53(5):673-84
references.pmid: 15545248
references.citation: Cladistics. 2013 Oct;29(5):466-542
references.pmid: 34798768
references.citation: Syst Biol. 2008 Feb;57(1):4-14
references.pmid: 18253896
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13410-4
references.pmid: 19633192
references.citation: J Evol Biol. 2012 Oct;25(10):2139-2148
references.pmid: 22905859
references.citation: Mol Phylogenet Evol. 2003 Jan;26(1):26-35
references.pmid: 12470935
references.citation: Mol Phylogenet Evol. 2008 Jul;48(1):224-39
references.pmid: 18394929
references.citation: Science. 2006 Apr 7;312(5770):101-4
references.pmid: 16601190
references.citation: Zootaxa. 2013;3647:201-50
references.pmid: 26295106
references.citation: Mol Phylogenet Evol. 2006 Mar;38(3):575-82
references.pmid: 16503279
references.citation: J Chem Ecol. 1989 Aug;15(8):2247-61
references.pmid: 24272384
references.citation: J Parasitol. 1992 Aug;78(4):573-87
references.pmid: 1635016
references.citation: Proc Natl Acad Sci U S A. 2005 May 24;102(21):7411-4
references.pmid: 15899976
references.citation: Annu Rev Entomol. 2013;58:609-30
references.pmid: 23317048
references.citation: Adv Parasitol. 2003;54:69-100
references.pmid: 14711084
references.citation: Biol Lett. 2007 Jun 22;3(3):275-9
references.pmid: 17363358
references.citation: Mol Phylogenet Evol. 2010 Jul;56(1):294-304
references.pmid: 20159044
references.citation: Syst Biol. 2012 Dec 1;61(6):1029-47
references.pmid: 22848088
references.citation: BMC Evol Biol. 2007 Nov 08;7:214
references.pmid: 17996036
references.citation: Mol Ecol. 2010 Apr;19(7):1483-96
references.pmid: 20456233
references.citation: PLoS One. 2011;6(11):e27023
references.pmid: 22087244
references.citation: Science. 2001 Apr 27;292(5517):686-93
references.pmid: 11326091
references.citation: Syst Biol. 2010 May;59(3):342-62
references.pmid: 20525640
references.citation: Syst Biol. 2011 Jan;60(1):3-15
references.pmid: 20952756
references.citation: Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13678-83
references.pmid: 11078527
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7329-34
references.pmid: 20368427
references.citation: Evolution. 2005 Nov;59(11):2299-311
references.pmid: 16396171
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12359-64
references.pmid: 18716001