title: Role of melatonin receptor MT(2) and quinone reductase II in the regulation of the redox status of 3T3-L1 preadipocytes in vitro.
abstract: We have examined the role of melatonin receptor MT2 and quinone reductase II in the regulation of the redox status of preadipocytes (3T3-L1) in vitro. 3T3-L1 cells were treated with melatonin at a physiological concentration (10(-9) mol/L) and a supraphysiological (pharmacological) concentration (10(-3) mol/L) for 24 h. Luzindole (10(-4) mol/L), an antagonist of MT2 receptor, and prazosin (10(-5) mol/L), an inhibitor of quinone reductase II, were added 30 min before subsequent exposure of the cells to melatonin. The level of oxidative stress was determined by the analysis of activities of enzymes neutralising reactive oxygen species, and determination of the malondialdehyde (MDA) content. Melatonin increased activities of manganese and copper-zinc superoxide dismutase (MnSOD, Cu/ZnSOD) and catalase (CAT) at both a physiological concentration (10(-9) mol/L) and a pharmacological concentration (10(-3) mol/L). MDA content was unchanged, whereas activities of glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-Rd) were increased only by the physiological concentration. Both effects were partially inhibited by luzindole, but not prazosin. These observations suggest that melatonin, acting at least partially via MT2 receptors, can increase antioxidant enzymes activities in 3T3-L1 preadipocytes.
authors.affiliation: Department of Neurology in Zabrze, Medical University of Silesia, ul. 3-go Maja 13-15, Zabrze, 41-800, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Adamczyk-Sowa
authors.forename: Pawel
authors.initials: P
authors.lastname: Sowa
authors.forename: Krystyna
authors.initials: K
authors.lastname: Zwirska-Korczala
authors.forename: Krystyna
authors.initials: K
authors.lastname: Pierzchala
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
authors.forename: Izabela
authors.initials: I
authors.lastname: Sadowska-Bartosz
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000502:physiology; D051379:Mice; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011224:Prazosin / Q000494:pharmacology; D011808:Quinone Reductases / Q000037:antagonists & inhibitors / Q000502:physiology*; D044123:Receptor, Melatonin, MT2 / Q000037:antagonists & inhibitors / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014363:Tryptamines / Q000494:pharmacology
chemical_list: D044123:Receptor, Melatonin, MT2; D014363:Tryptamines; C057154:luzindole; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C419642:NRH - quinone oxidoreductase2; D011808:Quinone Reductases; D005980:Glutathione Reductase; D008550:Melatonin; D011224:Prazosin
keywords: 3T3-L1 preadipocytes; antioxidant enzymes; luzindole; melatonin; oxidative stress; prazosin