title: Isoprene synthase genes form a monophyletic clade of acyclic terpene synthases in the TPS-B terpene synthase family.
abstract: Many plants emit significant amounts of isoprene, which is hypothesized to help leaves tolerate short episodes of high temperature. Isoprene emission is found in all major groups of land plants including mosses, ferns, gymnosperms, and angiosperms; however, within these groups isoprene emission is variable. The patchy distribution of isoprene emission implies an evolutionary pattern characterized by many origins or many losses. To better understand the evolution of isoprene emission, we examine the phylogenetic relationships among isoprene synthase and monoterpene synthase genes in the angiosperms. In this study we identify nine new isoprene synthases within the rosid angiosperms. We also document the capacity of a myrcene synthase in Humulus lupulus to produce isoprene. Isoprene synthases and (E)-β-ocimene synthases form a monophyletic group within the Tps-b clade of terpene synthases. No asterid genes fall within this clade. The chemistry of isoprene synthase and ocimene synthase is similar and likely affects the apparent relationships among Tps-b enzymes. The chronology of rosid evolution suggests a Cretaceous origin followed by many losses of isoprene synthase over the course of evolutionary history. The phylogenetic pattern of Tps-b genes indicates that isoprene emission from non-rosid angiosperms likely arose independently.
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA. tsharkey@msu.edu
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Sharkey
authors.forename: Dennis W
authors.initials: DW
authors.lastname: Gray
authors.forename: Heather K
authors.initials: HK
authors.lastname: Pell
authors.forename: Steven R
authors.initials: SR
authors.lastname: Breneman
authors.forename: Lauren
authors.initials: L
authors.lastname: Topper
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D019143:Evolution, Molecular; D017343:Genes, Plant; D027582:Humulus / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D013729:Terpenes / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C093854:isoprene synthase; C108877:terpene synthase