title: Preparation and enzymatic hydrolysis of nanoparticles made from single xyloglucan polysaccharide chain.
abstract: In this work, polysaccharide nanoparticles based on tamarind seeds xyloglucan are prepared, analyzed in term of characteristic sizes and morphology, and degraded by the action of a glycoside-hydrolase. Obtained in an aqueous NaNO2 solution (0.1M), these unaggregated nanoparticles have a characteristic diameter of ca. 60 nm (DLS, AFM and TEM measures). They are not compact, but highly swollen and look like hyperbranched and dendrimer-like (soft sphere model) structures. This observation is coherent with the native structure of the xyloglucan macromolecules which are themselves branched. The enzymatic hydrolysis by cellulase of Trichoderma reesei of the xyloglucan nanoparticles is investigated. In particular, the apparent mass molecular weight drastically decreases meaning that the xyloglucan nanoparticles are effectively fully hydrolyzed by the endo-β-(1,4)-glucanase. Furthermore, we observe that the enzyme has to uncoil the nanoparticles before cutting the β-(1→4) bonds and digesting the xyloglucan.
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales (CERMAV, CNRS UPR 5301), affiliated with the Université Joseph Fourier (UJF), member of the PolyNat Carnot Institute, 601 rue de la Chimie, BP 53, 38041 Grenoble cedex 9, France.
authors.forename: Ilham
authors.initials: I
authors.lastname: Mkedder
authors.forename: Christophe
authors.initials: C
authors.lastname: Travelet
authors.forename: Amandine
authors.initials: A
authors.lastname: Durand-Terrasson
authors.forename: Sami
authors.initials: S
authors.lastname: Halila
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dubreuil
authors.forename: Redouane
authors.initials: R
authors.lastname: Borsali
mesh_terms: D002240:Carbohydrate Sequence; D002480:Cellulase / Q000378:metabolism; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D008027:Light; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012542:Scattering, Radiation; D012977:Sodium Nitrite / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology; D014990:Xylans / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D002480:Cellulase; D012977:Sodium Nitrite