title: Efficient gene transfection in the neurotypic cells by star-shaped polymer consisting of β-cyclodextrin core and poly(amidoamine) dendron arms.
abstract: In order to develop the effective vectors that had high gene transfection capability and low cytotoxicity in the neuronal cells, we tested the star-shaped polymer consisting of β-cyclodextrin core and poly(amidoamine) (PAMAM) dendron arms [β-CD-(D3)7] as the vector to transfect the human neuroblastoma SH-SY5Y cells. The physicochemical properties of the β-CD-(D3)7/plasmid DNA (pDNA) complexes were characterized by using gel electrophoresis, dynamic light scattering, transmission electron microscopy and zeta-potential experiments. Among the human neuroblastoma SH-SY5Y cells, β-CD-(D3)7/pDNA complex demonstrated a lower toxicity compared to those of PAMAM (G=4, with an ethylenediamine core)/pDNA complex. When the N/P ratio was over 20, it was observed that PAMAM had a faster increment in toxicity compared to β-CD-(D3)7. Fluorescent image, confocal microscopy image and flow cytometry showed that β-CD-(D3)7/pDNA complexes had significantly higher transgene activity than that of PAMAM/pDNA complexes. For example, the transfection efficiency was 20% and 7.5% for β-CD-(D3)7/pDNA and PAMAM/pDNA complexes, respectively. These results indicated that β-CD-(D3)7 might be a promising candidate for neurotypic cells gene delivery with the characteristics of good biocompatibility, relatively high gene transfection capability and potential in vivo gene delivery ability.
authors.affiliation: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, Guangdong Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liang
authors.forename: Jun Jie
authors.initials: JJ
authors.lastname: Deng
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jian Rui
authors.initials: JR
authors.lastname: Yin
authors.forename: Shu Xiang
authors.initials: SX
authors.lastname: Pu
authors.forename: Long Chang
authors.initials: LC
authors.lastname: Xie
authors.forename: Cong
authors.initials: C
authors.lastname: Gao
authors.forename: Li Ming
authors.initials: LM
authors.lastname: Zhang
mesh_terms: D000873:Anthracenes / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008968:Molecular Conformation; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011073:Polyamines / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000633:toxicity
chemical_list: D000873:Anthracenes; C531249:Poly(amidoamine); D011073:Polyamines; D047392:beta-Cyclodextrins; C501244:dendron; D049452:Green Fluorescent Proteins; D004247:DNA