title: Genome characterisation of enteroviruses 117 and 118: a new group within human enterovirus species C.
abstract: The more than 120 genotypes of human enteroviruses (HEVs) reflect a wide range of evolutionary divergence, and there are 23 currently classified as human enterovirus C species (HEV-C). Two new HEV-C (EV-C117 and EV-C118) were identified in the Community-Acquired Pneumonia Pediatric Research Initiative (CAP-PRI) study, and the present paper describes the characterisation of the complete genome of one EV-C117 strain (LIT22) and two EV-C118 (ISR38 and ISR10) strains. The EV-C117 and EV-C118 5'UTR sequences were related to those of EV-C104, EV-C105 and EV-C109, and were slightly shorter than those of other HEV A-D species. Similarity plot analyses showed that EV-C117 and EV-C118 have a P1 region that is highly divergent from that of the other HEV-C, and phylogenetic analyses highly supported a monophyletic group consisting of EV-C117, EV-C118, EV-C104, EV-C105 and EV-C109 strains. Phylogenetic, Simplot and Bootscan analyses indicated that recombination was not the main mechanism of EV-C117 and EV-C118 evolution, thus strengthening the hypothesis of the monophyletic origin of the coding regions, as in the case of other HEV-C. Phylogenetic analysis also revealed the emergence of a new group within HEV-C that is divided into two subgroups. Nucleotide and amino acid identity in VP1 sequences have been established as useful criteria for assigning new HEV types, but analysis of the complete P1 region improves resolution.
authors.affiliation: Molecular Virology Unit, Fondazione Istituto Di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Piralla
authors.forename: Cristina
authors.initials: C
authors.lastname: Daleno
authors.forename: Alessia
authors.initials: A
authors.lastname: Scala
authors.forename: David
authors.initials: D
authors.lastname: Greenberg
authors.forename: Vytautas
authors.initials: V
authors.lastname: Usonis
authors.forename: Nicola
authors.initials: N
authors.lastname: Principi
authors.forename: Fausto
authors.initials: F
authors.lastname: Baldanti
authors.forename: Susanna
authors.initials: S
authors.lastname: Esposito
mesh_terms: D030015:Enterovirus C, Human / Q000145:classification* / Q000235:genetics*; D019143:Evolution, Molecular; D016679:Genome, Viral / Q000235:genetics*; D005838:Genotype; D006801:Humans; D010802:Phylogeny
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