title: Ethylene emission and PR protein synthesis in ACC deaminase producing Methylobacterium spp. inoculated tomato plants (Lycopersicon esculentum Mill.) challenged with Ralstonia solanacearum under greenhouse conditions.
abstract: Bacteria of genus Methylobacterium have been found to promote plant growth and regulate the level of ethylene in crop plants. This work is aimed to test the induction of defense responses in tomato against bacterial wilt by stress ethylene level reduction mediated by the ACC deaminase activity of Methylobacterium strains. Under greenhouse conditions, the disease index value in Methylobacterium sp. inoculated tomato plants was lower than control plants. Plants treated with Methylobacterium sp. challenge inoculated with Ralstonia solanacearum (RS) showed significantly reduced disease symptoms and lowered ethylene emission under greenhouse condition. The ACC and ACO (1-aminocyclopropane-1-carboxylate oxidase) accumulation in tomato leaves were significantly reduced with Methylobacterium strains inoculation. While ACC oxidase gene expression was found higher in plants treated with R. solanacearum than Methylobacterium sp. treatment, PR proteins related to induced systemic resistance like β-1,3-glucanase, PAL, PO and PPO were increased in Methylobacterium sp. inoculated plants. A significant increase in β-1,3-glucanase and PAL gene expression was found in all the Methylobacterium spp. treatments compared to the R. solanacearum treatment. This study confirms the activity of Methylobacterium sp. in increasing the defense enzymes by modulating the ethylene biosynthesis pathway and suggests the use of methylotrophic bacteria as potential biocontrol agents in tomato cultivation.
authors.affiliation: Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, South Korea.
authors.forename: Woojong
authors.initials: W
authors.lastname: Yim
authors.affiliation: Shri AMM Murugappa Chettiar Research Centre (MCRC), Taramani, Chennai 600113, India.
authors.forename: Sundaram
authors.initials: S
authors.lastname: Seshadri
authors.affiliation: Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, South Korea.
authors.forename: Kiyoon
authors.initials: K
authors.lastname: Kim
authors.affiliation: National Research Foundational of Korea, Daejeon 305-754, South Korea.
authors.forename: Gillseung
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, South Korea. Electronic address: tomsa@chungbuk.ac.kr.
authors.forename: Tongmin
authors.initials: T
authors.lastname: Sa
mesh_terms: D005030:Ethylenes / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000378:metabolism* / Q000382:microbiology*; D020580:Methylobacterium / Q000502:physiology*; D043368:Ralstonia solanacearum / Q000502:physiology*
chemical_list: D005030:Ethylenes; C036216:ethylene
keywords: ACC deaminase; Bacterial wilt; Ethylene; Methylobacterium; Plant growth promoting bacteria (PGPB); Ralstonia solanacearum