title: Primary research on unrelated double umbilical cord blood transplantation and implantation kinetics.
abstract: OBJECTIVE
  This study sought to examine implantation and implantation kinetics in double umbilical cord blood transplantation (DUCBT).
  
  
  METHODS
  Twenty-nine patients who underwent a two-unit unrelated donor cord blood transplantation were included in this study. After transplantation, hematopoietic chimerism of the peripheral blood was evaluated based on the results of short tandem repeat polymerase chain reaction. Using these results, we were able to judge whether the transplanted cells implanted, determine which donor's cells implanted, and further examine the kinetics of implantation in DUCBT. The numbers of total nucleated cells (TNCs), CD34(+) cells, colony forming units (CFUs), colony forming unit-granulocytes and macrophages (CFU-GMs), and CD3(+) cells were compared between the dominant units and the non-dominant units in an attempt to understand the discipline and implantation kinetics of DUCBT.
  
  
  RESULTS
  Neither the TNC counts nor the counts of CD34(+) cells, CFU, CFU-GM, or CD3(+) cells were significantly different between the dominant units and the non-dominant units (P values of 0.584, 0.322, 0.842, 0.534, and 0.082, respectively).
  
  
  CONCLUSIONS
  We were able to determine the engraftment status at 14 days after DUCBT, although the implantation kinetics of DUCBT remain uncharacterized and require further research.
authors.affiliation: Anhui Provincial Hospital, China.
authors.forename: Kaidi
authors.initials: K
authors.lastname: Song
authors.forename: Zimin
authors.initials: Z
authors.lastname: Sun
authors.forename: Huilan
authors.initials: H
authors.lastname: Liu
authors.forename: Liangquan
authors.initials: L
authors.lastname: Geng
authors.forename: Kaiyang
authors.initials: K
authors.lastname: Ding
authors.forename: Xingbing
authors.initials: X
authors.lastname: Wang
authors.forename: Baolin
authors.initials: B
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: Tong
authors.forename: Wen
authors.initials: W
authors.lastname: Yao
authors.forename: Xuhan
authors.initials: X
authors.lastname: Zhang
authors.forename: Zuyi
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D005260:Female; D006402:Hematologic Diseases / Q000601:surgery*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016019:Survival Analysis; D018183:Transplantation Chimera; D061349:Unrelated Donors; D055815:Young Adult