title: Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability.
abstract: The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation. Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system. In this study, twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection, regeneration capacity, and stable transgenic efficiency. Three genotypes, Yuechun 04-5, Yuechun 03-3, and Tianlong 1, showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system. For the Tianlong 1, the average stable transformation efficiency is 4.59%, higher than that of control genotypes (Jack and Williams 82), which is enough for further genomic research and genetic engineering. While polymerase chain reaction (PCR), LibertyLink strips, and β-glucuronidase (GUS) staining assays were used to detect the insertion and expression of the transgene, leaves painted with 135 mg/L Basta could efficiently identify the transformants.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhang-yue
authors.initials: ZY
authors.lastname: Song
authors.forename: Jing-luan
authors.initials: JL
authors.lastname: Tian
authors.forename: Wei-zhe
authors.initials: WZ
authors.lastname: Fu
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Ling-hong
authors.initials: LH
authors.lastname: Lu
authors.forename: Lian
authors.initials: L
authors.lastname: Zhou
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Shan
authors.forename: Gui-xiang
authors.initials: GX
authors.lastname: Tang
authors.forename: Hui-xia
authors.initials: HX
authors.lastname: Shou
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D005820:Genetic Testing; D005838:Genotype; D018515:Plant Leaves / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013025:Glycine max / Q000145:classification / Q000235:genetics* / Q000382:microbiology*; D014169:Transformation, Bacterial / Q000235:genetics*
chemical_list: D005819:Genetic Markers
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