title: [The change of transient receptor potential vanilloid-1 and interleukin-1α in periodontal tissue during experimental tooth movement].
abstract: OBJECTIVE
  To investigate the expression of TRPV1 and IL-1α in Sprague-Dawley rats' periodontal ligament (PLD) during experimental tooth movement of different orthodontic force and duration, and explore the role of TRPV1 and IL-1α in orthodontic pain.
  
  
  METHODS
  66 Sprague-Dawley rats were divided into control group (n=6), sham operation group (n=6) and experimental group (n=54) randomly. Orthodontic force was applied on right first maxillary molar in rats and the changes of TRPV1 and IL-1α expression were detected by real-time PCR at 4 h, 8 h, 1 d (three subgroups were added according to different forces: 1 d-30 g, 1 d-50 g, 1 d-80 g ), 3 d, 5 d, 7 d, 14 d after tooth movement. The data was analyzed using SPSS 13.0 software pakage.
  
  
  RESULTS
  Following the experimental tooth movement, the expression of TRPV1 and IL-1α in periodontal tissues were significantly up-regulated from 4 h to 7 d, with a peak at 1 d and returned to normal level at 1 week. The greater force applied during experimental tooth movement at 1 d, the higher expression of TRPV1 and IL-1α were detected in periodontal tissues.
  
  
  CONCLUSIONS
  Experimental tooth movement leads to regular change of TRPV1 and IL-1α expression in periodontal tissues, which indicates that TRPV1 and IL-1α may play an important role in orthodontic pain.
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, Shanxi Province, China. gaoyunan1111@stu.xjtu.edu.cn
authors.forename: Yu-Nan
authors.initials: YN
authors.lastname: Gao
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Zhu
authors.forename: Hu
authors.initials: H
authors.lastname: Qiao
mesh_terms: D000818:Animals; D053582:Interleukin-1alpha; D008437:Maxilla; D008963:Molar; D010513:Periodontal Ligament; D010519:Periodontium; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels; D014087:Tooth Movement Techniques
chemical_list: D053582:Interleukin-1alpha; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat