title: Gender-dependence of bone structure and properties in adult osteogenesis imperfecta murine model.
abstract: Osteogenesis imperfecta (OI) is a dominant skeletal disorder characterized by bone fragility and deformities. Though the oim mouse model has been the most widely studied of the OI models, it has only recently been suggested to exhibit gender-dependent differences in bone mineralization. To characterize the impact of gender on the morphometry/ultra-structure, mechanical properties, and biochemical composition of oim bone on the congenic C57BL/J6 background, 4-month-old oim/oim, +/oim, and wild-type (wt) female and male tibiae were evaluated using micro-computed tomography, three-point bending, and Raman spectroscopy. Dramatic gender differences were evident in both cortical and trabecular bone morphological and geometric parameters. Male mice had inherently more bone and increased moment of inertia than genotype-matched female counterparts with corresponding increases in bone biomechanical strength. The primary influence of gender was structure/geometry in bone growth and mechanical properties, whereas the mineral/matrix composition and hydroxyproline content of bone were influenced primarily by the oim collagen mutation. This study provides evidence of the importance of gender in the evaluation and interpretation of potential therapeutic strategies when using mouse models of OI.
authors.affiliation: Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri-Kansas City, 650 E. 25th St., Kansas City, MO 64108, USA.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Yao
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Carleton
authors.forename: Arin D
authors.initials: AD
authors.lastname: Kettle
authors.forename: Jennifer
authors.initials: J
authors.lastname: Melander
authors.forename: Charlotte L
authors.initials: CL
authors.lastname: Phillips
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002254:Carbonates / Q000378:metabolism; D004195:Disease Models, Animal; D055119:Elastic Modulus; D005260:Female; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010012:Osteogenesis; D010013:Osteogenesis Imperfecta / Q000000981:diagnostic imaging / Q000378:metabolism / Q000503:physiopathology*; D010710:Phosphates / Q000378:metabolism; D012737:Sex Factors; D013059:Spectrum Analysis, Raman; D013977:Tibia / Q000000981:diagnostic imaging / Q000502:physiology*; D055114:X-Ray Microtomography
chemical_list: D002254:Carbonates; D010710:Phosphates; D006909:Hydroxyproline
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