title: Rotational separation of non-spherical bioparticles using I-shaped pillar arrays in a microfluidic device.
abstract: Most bioparticles, such as red blood cells and bacteria, are non-spherical in shape. However, conventional microfluidic separation devices are designed for spherical particles. This poses a challenge in designing a separation device for non-spherical bioparticles, as the smallest dimension of the bioparticle has to be considered, which increases fabrication challenges and decreases the throughput. If current methods do not take into account the shape of non-spherical bioparticles, the separation will be inefficient. Here, to address this challenge, we present a novel technique for the separation of red blood cells as a non-spherical bioparticle, using a new I-shaped pillar arrays design. It takes the shape into account and induces rotational movements, allowing us to leverage on the largest dimension, which increases its separation size. This technique has been used for 100% separation of red blood cells from blood samples in a focused stream, outperforming the conventional pillar array designs.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Kerwin Kwek
authors.initials: KK
authors.lastname: Zeming
authors.forename: Shashi
authors.initials: S
authors.lastname: Ranjan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D044085:Microfluidics / Q000295:instrumentation*
references.citation: Lab Chip. 2011 Apr 7;11(7):1326-32
references.pmid: 21331436
references.citation: Phys Rev E Stat Nonlin Soft Matter Phys. 2008 Oct;78(4 Pt 2):046304
references.pmid: 18999523
references.citation: Lab Chip. 2012 Nov 7;12(21):4296-304
references.pmid: 22955667
references.citation: Lab Chip. 2006 May;6(5):655-8
references.pmid: 16652181
references.citation: Prenat Diagn. 2008 Oct;28(10):892-9
references.pmid: 18821715
references.citation: Biomicrofluidics. 2011 Apr 20;5(2):24103
references.pmid: 21584211
references.citation: Chem Soc Rev. 2010 Mar;39(3):1203-17
references.pmid: 20179832
references.citation: Phys Rev E Stat Nonlin Soft Matter Phys. 2011 May;83(5 Pt 2):056301
references.pmid: 21728641
references.citation: Mech Res Commun. 2009 Jan 1;36(1):92-103
references.pmid: 20046897
references.citation: Lab Chip. 2010 Oct 7;10(19):2605-13
references.pmid: 20689864
references.citation: Phys Rev Lett. 2009 Jan 30;102(4):045301
references.pmid: 19257437
references.citation: Lab Chip. 2007 Dec;7(12):1644-59
references.pmid: 18030382
references.citation: Lab Chip. 2012 Mar 21;12(6):1048-51
references.pmid: 22327631
references.citation: Science. 2004 May 14;304(5673):987-90
references.pmid: 15143275
references.citation: Lab Chip. 2012 Aug 21;12(16):2903-8
references.pmid: 22699242
references.citation: Anal Bioanal Chem. 2010 Aug;397(8):3249-67
references.pmid: 20419490
references.citation: Anal Chem. 2012 Sep 18;84(18):7954-62
references.pmid: 22897670
references.citation: Anal Chem. 2012 Dec 18;84(24):10621-7
references.pmid: 23137317
references.citation: Biomicrofluidics. 2012 Jun;6(2):24115-2411513
references.pmid: 22655023
references.citation: Biomicrofluidics. 2010 May 24;4(2):
references.pmid: 20697580
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14779-84
references.pmid: 17001005
references.citation: Lab Chip. 2005 Sep;5(9):922-9
references.pmid: 16100575
references.citation: Anal Chem. 2006 Jun 1;78(11):3765-71
references.pmid: 16737235