title: Cloning of the Xuhuai goat PPARγ gene and the preparation of transgenic sheep.
abstract: This study aimed to clone the peroxisome proliferator-activated receptor γ (PPARγ) gene of the Xuhuai goat and to make transgenic sheep using intratesticular injection, so as to improve the meat quality and flavor by increasing the intramuscular fat content. The coding sequence of the goat PPARγ gene was 1,428 bp, encoding 475 amino acids. Its similarity with other species was 81 (chicken), 89 (mouse), 92 (pig), 98 (cow), and 99% (sheep). The similarity of the corresponding amino acid sequences was 92.9, 97.3, 98.3, 99.6, and 99.8%, respectively. The signal peptide region of the PPARγ protein was not found in this study, demonstrating that the protein is not secreted. RT-PCR and western blot revealed that PPARγ was expressed in vitro, and the protein was localized in the cytoplasm. The PPARγ gene was expressed in F1 transgenic sheep at both the mRNA and the protein levels; the positive ratio was 13.7%.
authors.affiliation: Academy of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Qin
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Yani
authors.initials: Y
authors.lastname: Zhang
authors.forename: Caiye
authors.initials: C
authors.lastname: Zhu
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Shi
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zheng
authors.forename: Qi
authors.initials: Q
authors.lastname: Xu
authors.forename: Jiuzhou
authors.initials: J
authors.lastname: Song
authors.forename: Bichun
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D006041:Goats / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008460:Meat; D051379:Mice; D041681:NIH 3T3 Cells; D047495:PPAR gamma / Q000235:genetics*; D010957:Plasmids / Q000378:metabolism; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D034561:Sheep, Domestic / Q000235:genetics*; D013045:Species Specificity; D013552:Swine
chemical_list: D018076:DNA, Complementary; D047495:PPAR gamma; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins