title: Identification of compounds from Paris polyphylla (ChongLou) active against Dactylogyrus intermedius.
abstract: The present study was designated to ascertain the anthelmintic activity of the rhizomes of Paris polyphylla and to isolate and characterize the active constituents. The methanol extract from rhizomes of P. polyphylla showed signiﬁcant anthelmintic activity against Dactylogyrus intermedius with the median effective concentration (EC50) 22.5 mg L(-1). Based on this finding, the methanol extract was fractionated by silica gel column chromatography in a bioassay-guided fractionation yielding 2 bioactive compounds, the structures of these compounds were elucidated as formosanin C and polyphyllin VII. The in vivo tests revealed that formosanin C and polyphyllin VII were significantly effective against D. intermedius with EC50 values of 0.6 and 1.2 mg L(-1), respectively. The acute toxicities (LC50) of formosanin C and polyphyllin VII for grass carp were 2.8 and 2.9 mg L(-1), respectively. The overall results provide important information for the potential application of formosanin C and polyphyllin VII in the therapy of serious infection caused by D. intermedius.
authors.affiliation: College of Life Sciences, Jilin Agriculture University, Jilin, Changchun 130118, China.
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Li
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Wan
authors.forename: Gui-Qin
authors.initials: GQ
authors.lastname: Wang
authors.forename: Fu-Guang
authors.initials: FG
authors.lastname: Zhao
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Wen
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D017756:Aquaculture; D001681:Biological Assay / Q000662:veterinary; D002347:Carps; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D005393:Fish Diseases / Q000188:drug therapy* / Q000469:parasitology; D006374:Helminthiasis, Animal / Q000188:drug therapy* / Q000469:parasitology; D019684:Magnoliopsida / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D000432:Methanol / Q000737:chemistry; D021261:Parasitic Sensitivity Tests / Q000662:veterinary; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D010986:Platyhelminths / Q000187:drug effects*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D012503:Saponins; C585643:polyphyllin VII; C067584:formosanin C; D004144:Diosgenin; D000432:Methanol