title: Blind-deconvolution optical-resolution photoacoustic microscopy in vivo.
abstract: Optical-resolution photoacoustic microscopy (OR-PAM) is becoming a vital tool for studying the microcirculation system in vivo. By increasing the numerical aperture of optical focusing, the lateral resolution of OR-PAM can be improved; however, the depth of focus and thus the imaging range will be sacrificed correspondingly. In this work, we report our development of blind-deconvolution optical-resolution photoacoustic microscopy (BD-PAM) that can provide a lateral resolution ~2-fold finer than that of conventional OR-PAM (3.04 vs. 5.78μm), without physically increasing the system's numerical aperture. The improvement achieved with BD-PAM is demonstrated by imaging graphene nanoparticles and the microvasculature of mice ears in vivo. Our results suggest that BD-PAM may become a valuable tool for many biomedical applications that require both fine spatial resolution and extended depth of focus.
authors.affiliation: Research Laboratory for Biomedical Optics and Molecular Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Boulevard, Nanshan, Shenzhen 518055, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Chen
authors.forename: Riqiang
authors.initials: R
authors.lastname: Lin
authors.forename: Huina
authors.initials: H
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Meng
authors.forename: Hairong
authors.initials: H
authors.lastname: Zheng
authors.forename: Liang
authors.initials: L
authors.lastname: Song
mesh_terms: D000818:Animals; D004423:Ear / Q000033:anatomy & histology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D061088:Photoacoustic Techniques / Q000295:instrumentation*