title: Neutrophil-infiltrated paw edema induced by mannose-binding Dioclea violacea lectin.
abstract: BACKGROUND
  The potential edematogenic effect and the pharmacological characterization of a glucose-mannose-binding lectin from Dioclea violacea (DvL) were investigated.
  
  
  METHODS
  Paw edema was induced with DvL in control animals, and in animals pretreated with glucocorticoid or with blockers of histamine, nitric oxide synthase, cyclooxygenase, platelet activating factor (PAF), bradykinin and lipoxygenase.
  
  
  RESULTS
  DvL-induced paw edema paralleled with an increase in vascular permeability and myeloperoxidase (MPO) activity. DvL-induced edema could be prevented by pre-treatment with the lectin-binding sugar α-D-methyl mannoside. Dexamethasone, meclizine and Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) inhibited this effect.
  
  
  CONCLUSIONS
  DvL induces edema, increase in vascular permeability and neutrophil infiltration. The edematogenic activity involves the lectin mannose-binding sites and is associated with histamine, cytokines and nitric oxide, since it could be treated with meclizine, dexamethasone and L-NAME.
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceará, Cel. Nunes de Melo 1127, 60430-270, Fortaleza, CE, Brazil.
authors.forename: Nylane Maria N
authors.initials: NM
authors.lastname: de Alencar
authors.forename: Mário Rogério L
authors.initials: MR
authors.lastname: Mota
authors.forename: Natália V F C
authors.initials: NV
authors.lastname: Rodrigues
authors.forename: Jorge Luiz
authors.initials: JL
authors.lastname: Martins
authors.forename: Kyria S
authors.initials: KS
authors.lastname: do Nascimento
authors.forename: Ana Maria S
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001665:Binding Sites; D016207:Cytokines / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D052598:Dioclea / Q000737:chemistry*; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced* / Q000517:prevention & control; D005260:Female; D006632:Histamine / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000302:isolation & purification / Q000633:toxicity*; D008468:Meclizine / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D037601:Mannose-Binding Lectin; D009569:Nitric Oxide; D008468:Meclizine; D003907:Dexamethasone; D006632:Histamine; D009195:Peroxidase; D019331:NG-Nitroarginine Methyl Ester