title: Comparison of NIMS and MALDI platforms for neuropeptide and lipid mass spectrometric imaging inC. borealisbrain tissue.
abstract: Nanostructure-initiator mass spectrometry (NIMS) is a recently developed matrix-free laser desorption/ionization technique that has shown promise for peptide analyses. It is also useful in mass spectrometric imaging (MSI) studies of small molecule drugs, metabolites, and lipids, minimizing analyte diffusion caused by matrix application. In this study, NIMS and matrix-assisted laser desorption/ionization (MALDI) MSI of a crustacean model organismCancer borealisbrain were compared. MALDI was found to perform better than NIMS in these neuropeptide imaging experiments. Twelve neuropeptides were identified in MALDI MSI experiments whereas none were identified in NIMS MSI experiments. In addition, lipid profiles were compared using each ionization method. Both techniques provided similar lipid profiles in them/zrange 700 - 900.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Sturm
authors.forename: Tyler
authors.initials: T
authors.lastname: Greer
authors.forename: Ruibing
authors.initials: R
authors.lastname: Chen
authors.forename: Broderick
authors.initials: B
authors.lastname: Hensen
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
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