title: The FCRL3 -169T>C polymorphism and the risk of endometriosis-related infertility in a Polish population.
abstract: OBJECTIVE
  Recently, the FCRL3 -169T>C (rs7528684) single-nucleotide polymorphism (SNP) has been demonstrated to be a risk factor of endometriosis related infertility. We studied whether the FCRL -169T>C SNP can be associated with endometriosis-related infertility in a sample of the Polish population
  
  
  METHODS
  Using PCR-RFLP analysis we genotyped 141 infertile women with endometriosis and 519 fertile women. FCRL3 transcript levels were determined by reverse transcription and real-time quantitative PCR analysis in CD19(+) B cells from women with endometriosis-associated infertility and fertile women
  
  
  RESULTS
  We found a significantly increased frequency of the FCRL3 C/C genotype in women with endometriosis-associated infertility than controls [OR = 1.681 (95 % CI = 1.120-2.522, p = 0.0116, p corr = 0.0348)]. There was also a statistically increased frequency of the C/C and C/T genotypes in patients compared with controls [OR = 2.009 (95 % CI = 1.214-3.324, p = 0.0059, p corr = 0.0177)]. The p value of the χ (2) test for the trend observed for the FCRL3 -169T>C polymorphism was also statistically significant (p trend = 0.0012, p corr = 0.0036). We also found significantly increased FCRL3 transcript levels in carriers of the FCRL3 -169 CC vs TT and CT vs TT genotype both in women with endometriosis-related infertility (p = 0.012; p = 0.015) and fertile women (p = 0.017; p = 0.032) CONCLUSIONS: FCRL3 -169T>C polymorphism alters the expression of FCRL3 and can be a risk factor of endometriosis-related infertility.
authors.affiliation: Division of Reproduction, Department of Obstetrics, Gynecology and Gynecological Oncology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szczepańska
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Wirstlein
authors.forename: Hanna
authors.initials: H
authors.lastname: Hołysz
authors.forename: Jana
authors.initials: J
authors.lastname: Skrzypczak
authors.forename: Paweł P
authors.initials: PP
authors.lastname: Jagodziński
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000150:complications / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007247:Infertility, Female / Q000209:etiology* / Q000235:genetics; D016017:Odds Ratio; D011044:Poland; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D011971:Receptors, Immunologic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: C457775:FCRL3 protein, human; D005819:Genetic Markers; D011971:Receptors, Immunologic
references.citation: Fertil Steril. 1995 Mar;63(3):555-62
references.pmid: 7851586
references.citation: Indian J Med Res. 2011 Jun;133:581-93
references.pmid: 21727656
references.citation: J Soc Gynecol Investig. 2006 Oct;13(7):467-76
references.pmid: 16990031
references.citation: J Reprod Immunol. 2011 Mar;88(2):149-55
references.pmid: 21269709
references.citation: Fertil Steril. 2012 May;97(5):1124-8
references.pmid: 22341374
references.citation: Hum Reprod. 2002 Oct;17(10):2715-24
references.pmid: 12351553
references.citation: J Autoimmun. 2012 May;38(2-3):J266-74
references.pmid: 22284905
references.citation: Tissue Antigens. 2011 Mar;77(3):239-43
references.pmid: 21299530
references.citation: Am J Reprod Immunol. 2003 Jul;50(1):48-59
references.pmid: 14506928
references.citation: Arthritis Rheum. 2009 Nov;60(11):3510-2
references.pmid: 19877046
references.citation: Hum Immunol. 2011 Sep;72(9):774-8
references.pmid: 21663782
references.citation: N Engl J Med. 2009 Jan 15;360(3):268-79
references.pmid: 19144942
references.citation: Arq Bras Endocrinol Metabol. 2010 Aug;54(6):567-71
references.pmid: 20857063
references.citation: Hum Reprod. 1994 Apr;9(4):725-9
references.pmid: 8046030
references.citation: Am J Reprod Immunol. 2005 Feb;53(2):85-93
references.pmid: 15790342
references.citation: Nat Genet. 2005 May;37(5):478-85
references.pmid: 15838509
references.citation: Lancet. 2010 Aug 28;376(9742):730-8
references.pmid: 20801404
references.citation: Gynecol Endocrinol. 2011 Jun;27(6):425-33
references.pmid: 20586553
references.citation: Curr Med Chem. 2011;18(2):200-8
references.pmid: 21110806
references.citation: Fertil Steril. 1997 May;67(5):817-21
references.pmid: 9130884
references.citation: Hum Reprod Update. 2008 Sep-Oct;14(5):447-57
references.pmid: 18535005
references.citation: Fertil Steril. 2001 Jan;75(1):1-10
references.pmid: 11163805
references.citation: Headache. 2007 Jul-Aug;47(7):1069-78
references.pmid: 17635599
references.citation: Fertil Steril. 2011 Aug;96(2):512-5
references.pmid: 21733505
references.citation: Br J Obstet Gynaecol. 1991 Jul;98(7):680-4
references.pmid: 1883792
references.citation: Trends Mol Med. 2003 May;9(5):223-8
references.pmid: 12763528
references.citation: Hum Immunol. 2012 Mar;73(3):306-15
references.pmid: 22222816
references.citation: Fertil Steril. 1998 Jan;69(1):102-6
references.pmid: 9457942
references.citation: J Immunol. 2009 Nov 1;183(9):5502-10
references.pmid: 19843936
references.citation: Hum Reprod. 2003 Mar;18(3):544-9
references.pmid: 12615822
references.citation: J Reprod Immunol. 2011 May;89(2):212-5
references.pmid: 21529967
references.citation: Eur J Obstet Gynecol Reprod Biol. 2010 Jul;151(1):66-9
references.pmid: 20430510
references.citation: Fertil Steril. 1988 Nov;50(5):716-20
references.pmid: 3181483
references.citation: Anal Biochem. 1987 Apr;162(1):156-9
references.pmid: 2440339