title: Transcriptional profiling of rice early response to Magnaporthe oryzae identified OsWRKYs as important regulators in rice blast resistance.
abstract: Rice blast disease is a major threat to rice production worldwide, but the mechanisms underlying rice resistance to the causal agent Magnaporthe oryzae remain elusive. Therefore, we carried out a transcriptome study on rice early defense response to M. oryzae. We found that the transcriptional profiles of rice compatible and incompatible interactions with M. oryzae were mostly similar, with genes regulated more prominently in the incompatible interactions. The functional analysis showed that the genes involved in signaling and secondary metabolism were extensively up-regulated. In particular, WRKY transcription factor genes were significantly enriched among the up-regulated genes. Overexpressing one of these WRKY genes, OsWRKY47, in transgenic rice plants conferred enhanced resistance against rice blast fungus. Our results revealed the sophisticated transcriptional reprogramming of signaling and metabolic pathways during rice early response to M. oryzae and demonstrated the critical roles of WRKY transcription factors in rice blast resistance.
authors.affiliation: State Key Laboratory for Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wei
authors.forename: Bin
authors.initials: B
authors.lastname: Ou
authors.forename: Jinbin
authors.initials: J
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Dongshu
authors.initials: D
authors.lastname: Guo
authors.forename: Youyong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zhangliang
authors.initials: Z
authors.lastname: Chen
authors.forename: Hongya
authors.initials: H
authors.lastname: Gu
authors.forename: Chengyun
authors.initials: C
authors.lastname: Li
authors.forename: Genji
authors.initials: G
authors.lastname: Qin
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Qu
mesh_terms: D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D020082:Magnaporthe / Q000502:physiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
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