title: A preclinical xenograft model of prostate cancer using human tumors.
abstract: Most cases of prostate cancer are now diagnosed as moderate-grade localized disease. These tumor specimens are important tools in the discovery and translation of prostate cancer research; however, unlike more advanced tumors, they are notoriously difficult to grow in the laboratory. We developed a system for efficiently xenografting localized human prostate cancer tissue, and we adapted this protocol to study the interactions between the specific subsets of epithelial and stromal cells. Fresh prostate tissues or isolated epithelial cells are recombined with mouse seminal vesicle mesenchyme (SVM) and grafted under the renal capsule of immunodeficient mice for optimum growth and survival. Alternatively, mouse mesenchyme can be replaced with human prostate fibroblasts in order to determine their contribution to tumor progression. Grafts can be grown for several months to determine the effectiveness of novel therapeutic compounds when administered to host mice, thereby paving the way for personalizing the treatment of individual prostate cancers.
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Mitchell G
authors.initials: MG
authors.lastname: Lawrence
authors.forename: Renea A
authors.initials: RA
authors.lastname: Taylor
authors.forename: Roxanne
authors.initials: R
authors.lastname: Toivanen
authors.forename: John
authors.initials: J
authors.lastname: Pedersen
authors.forename: Sam
authors.initials: S
authors.lastname: Norden
authors.forename: David W
authors.initials: DW
authors.lastname: Pook
authors.forename: Mark
authors.initials: M
authors.lastname: Frydenberg
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Papargiris
authors.forename: Birunthi
authors.initials: B
authors.lastname: Niranjan
authors.forename: Michelle G
authors.initials: MG
authors.lastname: Richards
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Anne T
authors.initials: AT
authors.lastname: Collins
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Maitland
authors.forename: Gail P
authors.initials: GP
authors.lastname: Risbridger
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D018920:Coculture Techniques; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007668:Kidney / Q000601:surgery; D008297:Male; D051379:Mice; D016513:Mice, SCID; D011471:Prostatic Neoplasms / Q000473:pathology*; D012669:Seminal Vesicles / Q000473:pathology; D014183:Transplantation, Heterologous / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
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