title: Development of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus.
abstract: A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 10(6) copies/liter.
authors.affiliation: International Research Center for River Basin Environment, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Yamanashi, Japan. eharamoto@yamanashi.ac.jp
authors.forename: Eiji
authors.initials: E
authors.lastname: Haramoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitajima
authors.forename: Mikie
authors.initials: M
authors.lastname: Otagiri
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D010849:Picornaviridae / Q000145:classification / Q000235:genetics*; D010850:Picornaviridae Infections / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D045483:Rivers / Q000382:microbiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D012722:Sewage
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