title: The Arabidopsis general transcription factor TFIIB1 (AtTFIIB1) is required for pollen tube growth and endosperm development.
abstract: Pollen tube growth and endosperm development are important for fertilization and seed formation. The genetic mechanism of the processes remains poorly understood. This study reports the functional characterization of AtTFIIB1 in pollen tube growth and endosperm development. AtTFIIB1 shares 86% and 44% similarity with AtTFIIB2 and AtTFIIB3/AtpBRP2, respectively. It is expressed in many tissues including vegetative nuclei and generative cells of pollen grains and pollen tubes, endosperm, and embryos. It is thus different from AtTFIIB2, whose expression is not found in the endosperm and vegetative nucleus of mature pollen, and AtTFIIB3/AtpBRP2, which is expressed mostly in male gametophytes and weakly in seeds. Mutations in AtTFIIB1 caused a drastic retardation of pollen tube growth and endosperm development, as well as impaired pollen tube guidance and reception, leading to disruption of fertilization and seed development. Expression of AtTFIIB2 driven by the AtTFIIB1 promoter could restore the defective pollen tube growth, guidance, and reception completely, but only partially recovered the seed development in attfiib1, whilst expression of AtTFIIB3/AtpBRP2 driven by the AtTFIIB1 promoter could rescue only the defective attfiib1 seeds. All these results suggest that AtTFIIB1 plays important roles in pollen tube growth, guidance, and reception as well as endosperm development and is partially functionally different from AtTFIIB2 and AtTFIIB3/AtpBRP2.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Qian-Kun
authors.initials: QK
authors.lastname: Niu
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Chen
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.forename: De
authors.initials: D
authors.lastname: Ye
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D056625:Endosperm / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D053205:Pollen Tube / Q000235:genetics* / Q000254:growth & development; D054817:Pollination; D035581:Transcription Factor TFIIB / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C584932:TFIIB1 protein, Arabidopsis; D035581:Transcription Factor TFIIB; D014157:Transcription Factors
keywords: Arabidopsis; AtTFIIB1; endosperm; fertilization; pollen; transcription factor.
references.citation: Nature. 1995 Sep 14;377(6545):119-28
references.pmid: 7675079
references.citation: Plant Cell. 2005 Feb;17(2):584-96
references.pmid: 15659637
references.citation: Plant Physiol. 2000 Aug;123(4):1325-36
references.pmid: 10938351
references.citation: AoB Plants. 2011;2011:plr015
references.pmid: 22476485
references.citation: Science. 2007 Aug 3;317(5838):656-60
references.pmid: 17673660
references.citation: Genes Dev. 1996 Nov 1;10(21):2657-83
references.pmid: 8946909
references.citation: Plant Cell. 2000 Jan;12(1):125-36
references.pmid: 10634912
references.citation: Chromosoma. 2007 Oct;116(5):417-29
references.pmid: 17593382
references.citation: Plant J. 2009 Mar;57(5):870-82
references.pmid: 18980646
references.citation: Trends Biochem Sci. 1996 Sep;21(9):327-35
references.pmid: 8870495
references.citation: Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5628-32
references.pmid: 8516311
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Cell. 1996 Jan 26;84(2):245-52
references.pmid: 8565070
references.citation: Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5633-7
references.pmid: 8516312
references.citation: Development. 2008 Nov;135(21):3501-9
references.pmid: 18849529
references.citation: Plant J. 1996 Sep;10(3):561-8
references.pmid: 8811869
references.citation: J Integr Plant Biol. 2011 Jan;53(1):74-88
references.pmid: 21205178
references.citation: Microbiol Mol Biol Rev. 1998 Jun;62(2):465-503
references.pmid: 9618449
references.citation: Science. 2004 Feb 13;303(5660):983-8
references.pmid: 14963322
references.citation: J Exp Bot. 2011 Oct;62(14):5161-77
references.pmid: 21765162
references.citation: Biochem J. 2008 Apr 15;411(2):333-42
references.pmid: 18076381
references.citation: Plant J. 2010 Jul;63(2):189-202
references.pmid: 20444226
references.citation: Plant Cell. 2010 Feb;22(2):307-20
references.pmid: 20139161
references.citation: Plant J. 1998 Oct;16(1):21-31
references.pmid: 9807824
references.citation: Plant Physiol. 2008 Nov;148(3):1201-11
references.pmid: 18775970
references.citation: Plant Cell. 2004;16 Suppl:S214-27
references.pmid: 15010513
references.citation: PLoS One. 2011 Feb 24;6(2):e17216
references.pmid: 21390310
references.citation: Genes Dev. 1995 Jul 15;9(14):1797-810
references.pmid: 7622040
references.citation: Stain Technol. 1969 May;44(3):117-22
references.pmid: 4181665
references.citation: PLoS Genet. 2009 Aug;5(8):e1000621
references.pmid: 19714218
references.citation: BMC Mol Biol. 2007 Nov 12;8:102
references.pmid: 17997859
references.citation: Plant Physiol. 2008 Dec;148(4):1964-84
references.pmid: 18923020
references.citation: Genome Biol. 2004;5(11):R85
references.pmid: 15535861
references.citation: Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10637-42
references.pmid: 10962025
references.citation: Plant Mol Biol. 2002 Dec;50(6):925-47
references.pmid: 12516863
references.citation: Genome Res. 2008 Jul;18(7):1073-83
references.pmid: 18550805
references.citation: Development. 2009 Oct;136(19):3279-88
references.pmid: 19736323
references.citation: Nature. 2009 Nov 19;462(7271):323-30
references.pmid: 19820686
references.citation: Plant Cell. 2004 Aug;16(8):2020-34
references.pmid: 15295103