title: Pretreatment and hydrolysis methods for recovery of fermentable sugars from de-oiled Jatropha waste.
abstract: The release of reducing sugars (RS) upon various pretreatments and hydrolysis methods from de-oiled Jatropha waste (DJW) was studied. The highest RS concentration of 12.9 g/L was observed at 10% enzyme hydrolysis. The next highest RS of 8.0 g/L and 7.8 g/L were obtained with 10% HCl and 2.5% H2SO4, respectively. The NaOH (2.5%), ultrasonication and heat (90°C for 60 min) treatments showed the RS concentration of 2.5 g/L, 1.1 g/L and 2.0 g/L, respectively. Autoclave treatment slightly enhanced the sugar release (0.9 g/L) compared to no treatment (0.7 g/L). Glucose release (11.4 g/L) peaked in enzyme hydrolysis. Enzyme treated acid unhydrolysed biomass showed 11.1 g/L RS. HCl and H2SO4 pretreatment gave maximal xylose (6.89 g/L and 6.16 g/L, respectively). Combined (acid and enzyme) hydrolysis employed was efficient and its subsequent batch hydrogen fermentation showed a production 3.1 L H2/L reactor.
authors.affiliation: Department of Environmental Engineering and Science, Feng Chia University, Taichung 40724, Taiwan.
authors.forename: Gopalakrishnan
authors.initials: G
authors.lastname: Kumar
authors.forename: Biswarup
authors.initials: B
authors.lastname: Sen
authors.forename: Chiu-Yue
authors.initials: CY
authors.lastname: Lin
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006358:Hot Temperature; D006851:Hydrochloric Acid / Q000737:chemistry; D006859:Hydrogen / Q000378:metabolism; D006868:Hydrolysis; D031286:Jatropha / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry; D014465:Ultrasonics; D014866:Waste Products / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D013464:Sulfuric Acids; D014866:Waste Products; D012972:Sodium Hydroxide; D006859:Hydrogen; C033158:sulfuric acid; D006851:Hydrochloric Acid
keywords: Acid treatment; Alkali treatment; Enzyme hydrolysis; Hydrogen fermentation; Reducing sugars