title: [Study on associated biomarkers influencing recurrence, metastasis and prognosis in patients with gastrointestinal stromal tumors after complete resection].
abstract: OBJECTIVE
  To explore the associated biomarkers influencing recurrence, metastasis and prognosis in patients with gastrointestinal stromal tumors (GIST) after complete resection.
  
  
  METHODS
  Tumor tissue samples of 148 patients with GIST undergoing complete resection from January 1990 to December 2008 in Sun Yat-sen University Cancer Center were collected. The expressions of Ki-67, E-cadherin, MMP7, CD44, nm23, P53, survivin, Cyclin D1, COX-2, and VEGF in tumor tissue samples were detected by tissue microarray and immunohistochemistry (IHC). The association of above factors expressions with recurrence, metastasis and prognosis was examined.
  
  
  RESULTS
  Log-rank test showed that Ki-67, E-cadherin, MMP7, CD44, P53 and survivin were associated to disease-free duration after complete GIST resection (all P<0.05), and the Ki-67, E-cadherin, P53 and survivin were associated to overall survival (all P<0.05). Cox multivariate analysis revealed that disease-free survival was associated with Ki-67, CD44 and P53 (all P<0.05), and the overall survival was only associated with Ki-67 (P<0.05).
  
  
  CONCLUSIONS
  Ki-67, CD44 and P53 are closely associated with recurrence and metastasis after complete GIST resection, and Ki-67 can predict the prognosis of GIST.
authors.affiliation: Department of Colorectal Surgery, Cancer Center, Sun Yat-sen University, State Key Laboratory in Southern China, Guangzhou 510060, China.
authors.forename: Yu-jing
authors.initials: YJ
authors.lastname: Fang
authors.forename: Rong-xin
authors.initials: RX
authors.lastname: Zhang
authors.forename: Rong-zhen
authors.initials: RZ
authors.lastname: Luo
authors.forename: Qing-jian
authors.initials: QJ
authors.lastname: Ou
authors.forename: De-sen
authors.initials: DS
authors.lastname: Wan
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Pan
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000378:metabolism / Q000601:surgery*; D046152:Gastrointestinal Stromal Tumors / Q000378:metabolism / Q000601:surgery*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D019394:Ki-67 Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53