title: The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen.
abstract: In this study we evaluated the value of short-time insemination and early rescue intra-cytoplasmic sperm injection (ICSI) in preventing the occurrence of complete fertilisation failure for mild or moderate male infertility patients. A total of 866 couples with borderline semen who underwent in vitro fertilisation treatment in 2010 were included. Regular insemination was performed between January and June of 2010 and short-term insemination was performed from July through December 2010, where, as early as 4h after insemination, oocytes were denuded from cumulus cells and extrusion of the second polar body was evaluated. Of the 4153 mature oocytes with a detectable second polar body 4 h after insemination, 3874 (93.3%) showed signs of fertilisation on Day 1. Where no second polar body was present in any of the retrieved oocytes for a given patient, rescue ICSI was performed immediately. Similar rates of normal fertilisation and percentage of good-quality embryos were obtained between early rescue ICSI and regular ICSI. Clinical pregnancy occurred in 16 of 43 patients (37.2%) receiving early rescue ICSI. Our results showed early rescue ICSI in combination with evaluation of the second polar body 4 h following insemination is an effective method to prevent complete fertilisation failure for patients with mild or moderate male infertility.
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Shanjun
authors.initials: S
authors.lastname: Dai
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Shi
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Yingpu
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy*; D007315:Insemination, Artificial; D008297:Male; D059705:Polar Bodies; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D016879:Salvage Therapy; D055101:Semen Analysis; D012720:Severity of Illness Index; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000473:pathology*; D013997:Time Factors; D017211:Treatment Failure