title: Serum IL-10 from systemic lupus erythematosus patients suppresses the differentiation and function of monocyte-derived dendritic cells.
abstract: The role played by cytokines, other than interferon (IFN)-α, in the differentiation and function of dendritic cells (DCs) in systemic lupus erythematosus (SLE), remains unclear. Serum interleukin-10 (IL-10) levels are generally elevated in SLE patients, which might modulate the differentiation of DCs. In this study, DCs were induced from monocytes either by transendothelial trafficking or by culture with granulocyte-macrophage colony-stimulating factor (GM-CSF) + IL-4 + tumor necrosis factor (TNF)-α. Both systems were used to investigate the effects of elevated serum IL-10 level on DC differentiation in SLE patients. The results showed that monocyte-derived DCs induced by either SLE serum or exogenous IL-10 reduced the expression of human leukocyte antigen (HLA)-DR and CD80, decreased IL-12p40 level, and increased IL-10 level, and exhibited an impaired capacity to stimulate allogenic T-cell proliferation. These results indicate that serum IL-10 may be involved in the pathogenesis of SLE by modulating the differentiation and function of DCs.
authors.affiliation: Department of Oral Mucosal Diseases, the Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors.forename: Zhida
authors.initials: Z
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wang
authors.forename: Pengtao
authors.initials: P
authors.lastname: Jiang
authors.forename: Jiangquan
authors.initials: J
authors.lastname: Zhang
authors.forename: Mingshun
authors.initials: M
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Gu
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Yang
authors.forename: Miaojia
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ji
keywords: dendritic cells (DCs); differentiation; interleukin-10 (IL-10); lupus erythematosus systemic (SLE)
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