title: Construction and expression of an eukaryotic expression vector containing the IER3 gene.
abstract: BACKGROUND
  More and more research indicate that the immediately early response gene 3 (IER3) is involved in many biological processes, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression.
  
  
  METHODS
  Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Western- blotting.
  
  
  RESULTS
  Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls.
  
  
  CONCLUSIONS
  The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.
authors.affiliation: Department of Oncology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Yu
authors.forename: Ru-Yong
authors.initials: RY
authors.lastname: Yao
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Qiu
authors.forename: Lu
authors.initials: L
authors.lastname: Yue
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Sui
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Liu
authors.forename: Shi-Hai
authors.initials: SH
authors.lastname: Liu
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D004262:DNA Restriction Enzymes; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014162:Transfection
chemical_list: D051017:Apoptosis Regulatory Proteins; C099815:IER3 protein, human; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins; D004262:DNA Restriction Enzymes