title: Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development: advances and applications.
abstract: The cellulosome is one of nature's most elegant and elaborate nanomachines and a key biological and biotechnological macromolecule that can be used as a multi-functional protein complex tool. Each protein module in the cellulosome system is potentially useful in an advanced biotechnology application. The high-affinity interactions between the cohesin and dockerin domains can be used in protein-based biosensors to improve both sensitivity and selectivity. The scaffolding protein includes a carbohydrate-binding module (CBM) that attaches strongly to cellulose substrates and facilitates the purification of proteins fused with the dockerin module through a one-step CBM purification method. Although the surface layer homology (SLH) domain of CbpA is not present in other strains, replacement of the cell surface anchoring domain allows a foreign protein to be displayed on the surface of other strains. The development of a hydrolysis enzyme complex is a useful strategy for consolidated bioprocessing (CBP), enabling microorganisms with biomass hydrolysis activity. Thus, the development of various configurations of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications. This review provides a detailed summary of the current achievements in Clostridium-derived multi-functional complex development and the impact of these complexes in various areas of biotechnology.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Republic of Korea.
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Hyeon
authors.forename: Sang Duck
authors.initials: SD
authors.lastname: Jeon
authors.forename: Sung Ok
authors.initials: SO
authors.lastname: Han
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques; D001709:Biotechnology; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000235:genetics; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D002480:Cellulase; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009097:Multienzyme Complexes; D000097722:Cohesins
chemical_list: D002241:Carbohydrates; D018797:Cell Cycle Proteins; D002480:Cellulase; D002868:Chromosomal Proteins, Non-Histone; D009097:Multienzyme Complexes; D000097722:Cohesins; C473132:cellulosome, Clostridium
keywords: Biosensor; Carbohydrate binding module; Cell surface anchoring; Cellulosome; Clostridium cellulovorans; Cohesin–dockerin interaction; Consolidated bioprocessing; Designer minicellulosome; One-step CBM purification; Whole-cell biocatalyst