title: miRNA expression profiles of oral squamous cell carcinomas.
abstract: BACKGROUND
  Oral squamous cell carcinoma (OSCC) development involves complex machinery of genomic and epigenetic regulations including microRNA (miRNA) expression changes. We aimed to analyze the expression patterns of a set of miRNAs in human TNM stage I and II OSCC samples in an autologous normal mucosa-controlled experimental design.
  
  
  METHODS
  Forty samples of OSCC and 40 matched normal tissues were evaluated for miR-21, miR-155, miR-191, miR-146a, miR-221 and miR-222 expression in a LightCycler 480(PCR) system.
  
  
  RESULTS
  Our results showed significant overexpression of miR-21, miR-155, miR-191 and miR-221 in paired-sample t-test and the sensitivity/specificity of tests were over 90% in the case of miR-21 and miR-155 on the receiver operating characteristic curve (ROC) analysis.
  
  
  CONCLUSIONS
  Our results underline the role of miR-21 in OSCC and support the possible causal role of miR-155 and miR-221 in oral carcinogenesis. The overexpression of miR-191 is a novel finding in squamous cell carcinoma.
authors.affiliation: Institute of Public Health, Faculty of Medicine, Clinic Center University of Pécs, Pécs, Hungary. katalin_gombos@yahoo.com
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
authors.forename: Róbert
authors.initials: R
authors.lastname: Horváth
authors.forename: Eszter
authors.initials: E
authors.lastname: Szele
authors.forename: Krisztina
authors.initials: K
authors.lastname: Juhász
authors.forename: Katalin
authors.initials: K
authors.lastname: Gocze
authors.forename: Károly
authors.initials: K
authors.lastname: Somlai
authors.forename: Gábor
authors.initials: G
authors.lastname: Pajkos
authors.forename: István
authors.initials: I
authors.lastname: Ember
authors.forename: Lajos
authors.initials: L
authors.lastname: Olasz
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger