title: Regulation of podocalyxin expression in the kidney of streptozotocin-induced diabetic rats with Chinese herbs (Yishen capsule).
abstract: BACKGROUND
  Diabetic nephropathy is an emergent issue in China with increase in patients with type II diabetes. There are several successful Chinese herbal products for the treatment of patients with diabetic nephropathy in China. However, the mechanisms mediating the biological activity of these products are still unclear. Podocalyxin is a sialoprotein critical to maintaining integrity of filtration function of glomerulus.
  
  
  METHODS
  By employing streptozotocin-induced diabetic rats and a Chinese herb formulation (Yishen capsule), we examined the regulation of podocalyxin expression in the kidney by Yishen capsule through immunofluorescent staining and reverse transcriptase polymerase chain reaction.
  
  
  RESULTS
  After injection of STZ, there were significant increase in both blood glucose and urinary protein. Serum creatinine and BUN were also increased in rats with injection of STZ. Moreover, expression of podocalyxin in the glomerulus was gradually reduced after injection of STZ. There was also a loss of podocyte foot processes in the glomerular basement membrane. However, Yishen capsule or benazepril was able to restore the expression of podocalyxin and podocyte foot processes in the kidney. Although Yishen capsule could reduce urinary protein level, it has little effect on blood glucose level in the rats injected with STZ.
  
  
  CONCLUSIONS
  Yishen capsule could attenuate the loss of podocalyxin in the glomerulus of rats injected with STZ.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital to the Shanxi Medical University, 56 Xinjian South Road, Taiyuan, Shanxi Province 030001, People's Republic of China. fja3455001@126.com
authors.forename: Jingai
authors.initials: J
authors.lastname: Fang
authors.forename: Hongkun
authors.initials: H
authors.lastname: Wei
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Liu
authors.forename: Qintao
authors.initials: Q
authors.lastname: Chang
authors.forename: Ruihua
authors.initials: R
authors.lastname: Wang
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Gong
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D012795:Sialoglycoproteins; C001643:podocalyxin; C525740:yishen
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