title: Microfluidic space-domain time-resolved emission spectroscopy of terbium(III) and europium(III) chelates with pyridine-2,6-dicarboxylate.
abstract: This article describes the utilization of laminar microflows for time-resolved emission measurements with steady-state excitation and detection. Passing a laminar flow through a short illuminated section of a microchannel provided a means for pulsed-like photoexcitation of the moieties carried by the fluid. Imaging the microchannel flows carrying thus photoexcited chelates of lanthanide ions allowed us to extract their excited-state lifetimes from the spatial distribution of the changes in the emission intensity. The lifetime values obtained using this space-domain approach agreed well with the lifetimes from time-domain measurements. This validated space-domain microfluidic approach reveals a means for miniaturization of time-resolved emission spectroscopy.
authors.affiliation: Department of Bioengineering and Center for Bioengineering Research, University of California, Riverside, California 92521, United States.
authors.forename: Vicente
authors.initials: V
authors.lastname: Nuñez
authors.forename: Srigokul
authors.initials: S
authors.lastname: Upadhyayula
authors.forename: Brent
authors.initials: B
authors.lastname: Millare
authors.forename: Jillian M
authors.initials: JM
authors.lastname: Larsen
authors.forename: Ali
authors.initials: A
authors.lastname: Hadian
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Shin
authors.forename: Prashanthi
authors.initials: P
authors.lastname: Vandrangi
authors.forename: Sharad
authors.initials: S
authors.lastname: Gupta
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Adam P
authors.initials: AP
authors.lastname: Lin
authors.forename: Georgi Y
authors.initials: GY
authors.lastname: Georgiev
authors.forename: Valentine I
authors.initials: VI
authors.lastname: Vullev
mesh_terms: D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008856:Microscopy, Fluorescence; D010848:Picolinic Acids / Q000737:chemistry*; D013725:Terbium / Q000737:chemistry*; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D010848:Picolinic Acids; D013725:Terbium; D005063:Europium; C004860:dipicolinic acid