title: Use of transgenic animals in biotechnology: prospects and problems.
abstract: During the past two decades, there have been numerous attempts at using animals in order to produce recombinant human proteins and monoclonal antibodies. However, it is only recently that the first two therapeutic agents isolated from the milk of transgenic animals, C1 inhibitor (Ruconest) and antithrombin (ATryn), appeared on the market. This inspires hope that a considerable number of new recombinant proteins created using such technology could become available for practical use in the near future. In this review, the methods applied to produce transgenic animals are described and the advantages and drawbacks related to their use for producing recombinant human proteins and monoclonal antibodies are discussed.
authors.affiliation: Institute of Gene Biology of the Russian Academy of Sciences, Vavilov St., 34/5, Moscow, Russia, 119334.
authors.forename: O G
authors.initials: OG
authors.lastname: Maksimenko
authors.forename: A V
authors.initials: AV
authors.lastname: Deykin
authors.forename: Yu M
authors.initials: YM
authors.lastname: Khodarovich
authors.forename: P G
authors.initials: PG
authors.lastname: Georgiev
keywords: bioreactor; milk protein production; production of monoclonal antibodies; recombinant proteins; therapeutic drugs; transgenic animals
references.citation: Nature. 1987 Aug 6-12;328(6130):530-2
references.pmid: 3614356
references.citation: Transgenic Res. 2009 Aug;18(4):573-82
references.pmid: 19219636
references.citation: J Biol Chem. 2009 Aug 21;284(34):22815-24
references.pmid: 19542223
references.citation: Transgenic Res. 2003 Dec;12(6):723-30
references.pmid: 14713201
references.citation: Nat Biotechnol. 2001 Aug;19(8):741-5
references.pmid: 11479566
references.citation: Transgenic Res. 2009 Jun;18(3):445-53
references.pmid: 19130282
references.citation: Curr Opin Biotechnol. 2002 Dec;13(6):625-9
references.pmid: 12482525
references.citation: Mol Cell Biol. 1991 Jun;11(6):3070-4
references.pmid: 2038318
references.citation: Nature. 1985 May 30-Jun 5;315(6018):413-6
references.pmid: 3923368
references.citation: Nat Biotechnol. 2003 May;21(5):553-8
references.pmid: 12679786
references.citation: Curr Opin Biotechnol. 1996 Oct;7(5):536-40
references.pmid: 8939621
references.citation: Transgenic Res. 2009 Oct;18(5):747-56
references.pmid: 19396561
references.citation: J Nutr. 2008 May;138(5):921-6
references.pmid: 18424602
references.citation: Biotechnol Annu Rev. 1998;4:1-54
references.pmid: 9890137
references.citation: Immunotechnology. 1995 Aug;1(2):107-13
references.pmid: 9373339
references.citation: Transgenic Res. 2011 Apr;20(2):417-9
references.pmid: 20549346
references.citation: Basic Life Sci. 1986;37:95-111
references.pmid: 3010937
references.citation: Development. 2012 Mar;139(6):1045-57
references.pmid: 22354838
references.citation: Trends Biotechnol. 2003 Sep;21(9):394-9
references.pmid: 12948672
references.citation: Protein Expr Purif. 2007 May;53(1):225-31
references.pmid: 17208010
references.citation: J Mol Cell Biol. 2011 Apr;3(2):132-41
references.pmid: 21149239
references.citation: J Anim Sci. 1991 Nov;69(11):4552-62
references.pmid: 1721617
references.citation: Reprod Suppl. 2001;58:313-24
references.pmid: 11980200
references.citation: Transgenic Res. 2008 Aug;17(4):727-32
references.pmid: 18183493
references.citation: J Biotechnol. 2004 Oct 19;114(1-2):89-97
references.pmid: 15464602
references.citation: Glycobiology. 2008 Jul;18(7):526-39
references.pmid: 18456721
references.citation: Transgenic Res. 2011 Aug;20(4):933-8
references.pmid: 21107689
references.citation: Nat Biotechnol. 2001 Jan;19(1):66-70
references.pmid: 11135555
references.citation: Transgenic Res. 2003 Dec;12(6):751-5
references.pmid: 14713206
references.citation: J Dairy Sci. 2006 Feb;89(2):518-24
references.pmid: 16428620
references.citation: DNA Cell Biol. 2003 Jan;22(1):41-5
references.pmid: 12590736
references.citation: J Virol. 1998 May;72(5):3762-72
references.pmid: 9557658
references.citation: Mol Cell. 2004 Nov 5;16(3):453-63
references.pmid: 15525517
references.citation: Transgenic Res. 2012 Oct;21(5):1085-91
references.pmid: 22286336
references.citation: Nat Biotechnol. 2007 Jan;25(1):132-8
references.pmid: 17195841
references.citation: J Immunoassay Immunochem. 2009;30(3):262-90
references.pmid: 19591041
references.citation: Eur J Biochem. 1997 Apr 15;245(2):482-9
references.pmid: 9151983
references.citation: Cytotechnology. 2002 Jan;38(1-3):43-6
references.pmid: 19003085
references.citation: Biochem Cell Biol. 2012 Jun;90(3):513-9
references.pmid: 22360490
references.citation: Gene. 2007 Oct 15;401(1-2):97-107
references.pmid: 17692477
references.citation: Transgenic Res. 1994 Jan;3(1):36-42
references.pmid: 8142951
references.citation: PLoS One. 2011;6(8):e23573
references.pmid: 21897845
references.citation: Transgenic Res. 2010 Jun;19(3):357-61
references.pmid: 19888667
references.citation: Biotechnol Adv. 2012 Nov-Dec;30(6):1336-43
references.pmid: 22361646
references.citation: Transgenic Res. 2005 Aug;14(4):417-28
references.pmid: 16201408
references.citation: Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13603-8
references.pmid: 17660298
references.citation: Foodborne Pathog Dis. 2006 Winter;3(4):384-92
references.pmid: 17199520
references.citation: Mamm Genome. 2007 Oct;18(10):693-708
references.pmid: 17882484
references.citation: Vox Sang. 2011 Jan;100(1):68-83
references.pmid: 21175657
references.citation: Transgenic Res. 1994 Mar;3(2):99-108
references.pmid: 8193642
references.citation: Mol Reprod Dev. 2011 Oct-Nov;78(10-11):879-91
references.pmid: 21671302
references.citation: Transgenic Res. 2009 Oct;18(5):769-85
references.pmid: 19396621
references.citation: Nat Biotechnol. 1998 Apr;16(4):349-54
references.pmid: 9555725
references.citation: Res Immunol. 1998 Jul-Aug;149(6):609-10
references.pmid: 9835426
references.citation: J Biotechnol. 2006 Jul 13;124(2):469-72
references.pmid: 16716426
references.citation: Biotechnol Adv. 2009 May-Jun;27(3):297-306
references.pmid: 19500547
references.citation: Comp Immunol Microbiol Infect Dis. 2009 Mar;32(2):107-21
references.pmid: 18243312
references.citation: Nature. 1997 Feb 27;385(6619):810-3
references.pmid: 9039911
references.citation: PLoS One. 2008;3(10):e3453
references.pmid: 18941633
references.citation: Nat Biotechnol. 2003 Feb;21(2):157-62
references.pmid: 12548290
references.citation: Transgenic Res. 1991 Dec;1(1):3-13
references.pmid: 1844572
references.citation: Trends Biotechnol. 1999 Sep;17(9):367-74
references.pmid: 10461183
references.citation: Trends Biotechnol. 2006 Mar;24(3):137-42
references.pmid: 16460822
references.citation: Transgenic Res. 2012 Jun;21(3):485-98
references.pmid: 21881921
references.citation: Biotechnol Appl Biochem. 2010 Apr 07;55(4):175-89
references.pmid: 20392202
references.citation: J Pharm Sci. 2009 Oct;98(10):3499-508
references.pmid: 19199295
references.citation: Nat Biotechnol. 2002 May;20(5):484-7
references.pmid: 11981562
references.citation: Transgenic Res. 1997 May;6(3):203-12
references.pmid: 9167268
references.citation: Glycobiology. 2004 Jan;14(1):51-64
references.pmid: 14514717
references.citation: J Biol Chem. 2003 Jan 31;278(5):3466-73
references.pmid: 12427744
references.citation: Transgenic Res. 2010 Oct;19(5):923-31
references.pmid: 20107893
references.citation: J Immunol Methods. 1999 Dec 10;231(1-2):147-57
references.pmid: 10648934
references.citation: Glycobiology. 2000 May;10(5):477-86
references.pmid: 10764836
references.citation: Trends Biotechnol. 2010 May;28(5):253-61
references.pmid: 20304511
references.citation: Curr Opin Biotechnol. 2009 Dec;20(6):700-7
references.pmid: 19889531
references.citation: Blood. 1998 Jun 15;91(12):4561-71
references.pmid: 9616152
references.citation: J Dairy Sci. 1997 Sep;80(9):2225-32
references.pmid: 9313168
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 10;99 Suppl 4:16378-83
references.pmid: 12181490
references.citation: Transgenic Res. 2007 Jun;16(3):353-61
references.pmid: 17265165
references.citation: Bioessays. 2004 Jul;26(7):796-807
references.pmid: 15221861
references.citation: Transgenic Res. 2011 Dec;20(6):1265-72
references.pmid: 21340524
references.citation: Biotechnology (N Y). 1994 Jul;12(7):699-702
references.pmid: 7764915
references.citation: PLoS Genet. 2010 Jan;6(1):e1000804
references.pmid: 20062523
references.citation: Nat Biotechnol. 2005 Apr;23(4):445-51
references.pmid: 15806099
references.citation: Protein Expr Purif. 2008 Feb;57(2):127-35
references.pmid: 18054499
references.citation: Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):575-80
references.pmid: 9012826
references.citation: J Dairy Sci. 2008 Dec;91(12):4466-76
references.pmid: 19038921
references.citation: Biotechnol Bioeng. 2004 Aug 5;87(3):274-85
references.pmid: 15281102
references.citation: Biotechnol Bioeng. 2005 Jul 5;91(1):1-11
references.pmid: 15889435
references.citation: Biochem Genet. 2006 Oct;44(7-8):349-60
references.pmid: 17028784
references.citation: Biochem Biophys Res Commun. 2000 Jul 14;273(3):1015-8
references.pmid: 10891364
references.citation: Dokl Biochem Biophys. 2009 Jul-Aug;427:195-8
references.pmid: 19817135
references.citation: Reprod Nutr Dev. 2006 Sep-Oct;46(5):579-88
references.pmid: 17107647
references.citation: Prev Vet Med. 2011 Nov 1;102(2):146-56
references.pmid: 21601297
references.citation: Genet Anal. 1999 Nov;15(3-5):155-60
references.pmid: 10596756
references.citation: Nat Biotechnol. 2011 Aug 05;29(8):706-10
references.pmid: 21822244
references.citation: Transgenic Res. 2000 Jun;9(3):215-22
references.pmid: 11032370
references.citation: J Biotechnol. 2006 May 17;123(2):225-35
references.pmid: 16338016
references.citation: Cell. 1999 Aug 6;98(3):387-96
references.pmid: 10458613
references.citation: Nat Methods. 2007 Sep;4(9):747-53
references.pmid: 17676049
references.citation: Transgenic Res. 2009 Jun;18(3):361-76
references.pmid: 19031005
references.citation: Nucleic Acids Res. 2003 Nov 1;31(21):6290-305
references.pmid: 14576318
references.citation: Protein Expr Purif. 2004 Dec;38(2):177-83
references.pmid: 15555933
references.citation: Cold Spring Harb Symp Quant Biol. 2010;75:79-85
references.pmid: 21047907
references.citation: Genetika. 2006 Aug;42(8):1029-44
references.pmid: 17025153