title: Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus).
abstract: As an essential member of the HSP70 family, heat shock cognate 70 (Hsc70) is a constitutively expressed molecular chaperone involved in protein metabolism. In this paper, turbot Hsc70 was cloned and the expression profile was also analyzed. The full-length cDNA of the turbot Hsc70 was 2,292 bp in length, including a 113-bp 5' UTR, a 223-bp 3' UTR and a 1,956-bp open reading frame coding a protein with 651 amino acid residues. Comparison of amino acid sequence revealed the existence of three classical HSP70 family signature motifs, a signature nonapeptide and one repeat of tetrapeptide in turbot Hsc70. The turbot Hsc70-deduced amino acids sequence exhibited 75.4-96.8 % homology with Hsp70s/Hsc70s of 24 other known sequences. In particular, the strongest homology was found with the cognate members of Hsc70 subfamily and the highest identity was found with Japanese flounder Hsc70. Semi-quantitative RT-PCR revealed that turbot Hsc70 transcripts were stably expressed in all tested tissues under normal physiological condition, while the expression levels also increased (~1.5-fold to ~threefold) after heat shock and bacterial infection. In addition, Hsc70 transcripts were detected throughout embryonic development and in turbot embryonic cell line (TEC) in the absence of any stress. Meanwhile, it was also heat inducible, but not cold inducible in TEC. These results suggest that Hsc70 gene may be involved in embryogenesis and cellular protection events under normal and stress condition.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071, China.
authors.forename: T T
authors.initials: TT
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Wang
authors.forename: X L
authors.initials: XL
authors.lastname: Liao
authors.forename: L
authors.initials: L
authors.lastname: Meng
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D047108:Embryonic Development; D005412:Flatfishes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D050883:HSC70 Heat-Shock Proteins
references.citation: Dis Aquat Organ. 2004 Dec 13;62(3):205-15
references.pmid: 15672876
references.citation: Dev Genet. 1997;21(3):223-33
references.pmid: 9397538
references.citation: Nature. 1996 Jun 13;381(6583):571-9
references.pmid: 8637592
references.citation: EMBO J. 2008 Jan 23;27(2):328-35
references.pmid: 18216875
references.citation: Genetica. 1996-1997;98(3):273-6
references.pmid: 9204550
references.citation: Aquat Toxicol. 2003 Oct 8;65(1):39-54
references.pmid: 12932700
references.citation: FASEB J. 2006 Jul;20(9):1549-51
references.pmid: 16723383
references.citation: Biol Res. 2005;38(1):69-82
references.pmid: 15977412
references.citation: J Immunol. 2005 Sep 1;175(5):2900-12
references.pmid: 16116176
references.citation: Cancer Res. 1999 Sep 1;59(17):4219-21
references.pmid: 10485459
references.citation: Gene. 2001 Feb 21;264(2):197-203
references.pmid: 11250074
references.citation: J Immunol. 1990 May 15;144(10):3987-91
references.pmid: 2110215
references.citation: Cell Stress Chaperones. 2004 Autumn;9(3):313-23
references.pmid: 15544169
references.citation: Science. 2002 Mar 8;295(5561):1852-8
references.pmid: 11884745
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2002 Jun;132(2):223-33
references.pmid: 12106899
references.citation: Annu Rev Genet. 1988;22:631-77
references.pmid: 2853609
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2003 Oct;136(2):275-82
references.pmid: 14529753
references.citation: Am J Physiol Regul Integr Comp Physiol. 2004 Aug;287(2):R429-36
references.pmid: 15117724
references.citation: Biochem Biophys Res Commun. 2005 Sep 30;335(3):799-809
references.pmid: 16098479
references.citation: Biochem Biophys Res Commun. 2005 May 13;330(3):776-83
references.pmid: 15809064
references.citation: Biochim Biophys Acta. 1999 Mar 19;1444(3):315-25
references.pmid: 10095055
references.citation: Biochem Biophys Res Commun. 2003 Aug 1;307(3):503-9
references.pmid: 12893250
references.citation: Jpn J Genet. 1995 Jun;70(3):423-33
references.pmid: 7632444
references.citation: Plant Cell. 2007 Dec;19(12):4061-76
references.pmid: 18065690
references.citation: Blood. 1997 Aug 15;90(4):1516-26
references.pmid: 9269769
references.citation: DNA Seq. 2000;11(3-4):265-70
references.pmid: 11092738
references.citation: Biol Bull. 2002 Dec;203(3):331-7
references.pmid: 12480723
references.citation: J Cell Sci. 2004 Jun 1;117(Pt 13):2641-51
references.pmid: 15169835
references.citation: Fish Shellfish Immunol. 2007 Mar;22(3):172-81
references.pmid: 16908195
references.citation: J Endocrinol. 1999 Jun;161(3):413-21
references.pmid: 10333544
references.citation: Int J Mol Med. 1999 Nov;4(5):463-74
references.pmid: 10534567
references.citation: J Endocrinol. 2006 Aug;190(2):433-40
references.pmid: 16899576
references.citation: Eur J Appl Physiol. 2009 Apr;105(6):909-17
references.pmid: 19125275
references.citation: Mol Cell Biol. 1998 Nov;18(11):6238-44
references.pmid: 9774640
references.citation: J Toxicol Environ Health A. 2007 Jun;70(12):1005-13
references.pmid: 17497411
references.citation: Eur J Biochem. 1992 Mar 1;204(2):893-900
references.pmid: 1371753
references.citation: Aquat Toxicol. 2006 Aug 12;79(1):9-15
references.pmid: 16780970
references.citation: Cell. 2000 Apr 14;101(2):199-210
references.pmid: 10786835
references.citation: Eur J Pharmacol. 2012 May 15;683(1-3):10-5
references.pmid: 22387093
references.citation: Cell Stress Chaperones. 2004 Winter;9(4):332-43
references.pmid: 15633291
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2003 Apr;134(4):839-46
references.pmid: 12814792
references.citation: J Neurosci Res. 2007 Feb 1;85(2):402-9
references.pmid: 17203483
references.citation: Annu Rev Physiol. 1999;61:243-82
references.pmid: 10099689
references.citation: J Cell Biol. 1993 Mar;120(5):1101-12
references.pmid: 8436586
references.citation: Cell. 1986 Apr 11;45(1):3-13
references.pmid: 2937542