title: MiRNA-429 suppresses the growth of gastric cancer cells in vitro.
abstract: Micro-RNAs (miRNAs) have been found to be implicated in a very wide range of physiological processes. This study was aimed to investigate the regulation of miRNA-429 (miR-429) in gastric cancer cells on cell proliferation and apoptosis. Quantitative PCR was employed to detect the expressions of miR-429 after eukaryotic expression plasmid of miR-429 and its inhibitor were transiently transfected into poorly differentiated human gastric cancer cell line BGC823. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assays were used to examine proliferation ability. Apoptosis was analyzed by flow cytometry after transfection. The results showed that 48 h after transfection, overexpression of miR-429 reached maximum efficiency. Compared with mock transfection, miR-429 inhibited tumor cell proliferation significantly (P < 0.05) at 48 h and 72 h. of Overexpression of miR-429 promoted tumor cell apoptosis when compared with mock transfected cells (P < 0.05). On the contrary, miR-429 inhibitor promoted tumor cell proliferation and inhibited apoptosis when compared with controls (P < 0.05). Our results suggested that miRNA-429 may serve as a tumor suppressor during tumorigenesis of gastric cancer and may be a potential gastric cancer therapeutic target.
authors.affiliation: Department of Surgical Oncology, the First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China;
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Xia
authors.forename: Dongmei
authors.initials: D
authors.lastname: Diao
authors.forename: Yao
authors.initials: Y
authors.lastname: Cheng
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Dawei
authors.initials: D
authors.lastname: Yuan
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.forename: Chengxue
authors.initials: C
authors.lastname: Dang
keywords: apoptosis; cell cycle; gastric cancer; microRNA
references.citation: Nat Med. 2011 Dec 11;18(1):74-82
references.pmid: 22157681
references.citation: Clin Cancer Res. 2011 Aug 15;17(16):5287-98
references.pmid: 21159887
references.citation: Cancer Res. 2008 Jan 15;68(2):425-33
references.pmid: 18199536
references.citation: J Pathol. 2011 Jan;223(2):102-15
references.pmid: 21125669
references.citation: Gynecol Oncol. 2009 Sep;114(3):457-64
references.pmid: 19501389
references.citation: Oncogene. 2007 Apr 26;26(19):2799-803
references.pmid: 17072344
references.citation: Br J Haematol. 2008 May;141(5):672-5
references.pmid: 18318758
references.citation: Carcinogenesis. 2010 Jun;31(6):1037-44
references.pmid: 20351093
references.citation: PLoS One. 2011;6(12):e29043
references.pmid: 22194984
references.citation: Annu Rev Biochem. 2010;79:351-79
references.pmid: 20533884
references.citation: Cancer Res. 2010 Nov 15;70(22):9473-82
references.pmid: 21062975
references.citation: Expert Rev Mol Diagn. 2011 Nov;11(8):813-27
references.pmid: 22022944
references.citation: Eur J Cancer. 2011 Jan;47(2):163-74
references.pmid: 21145728
references.citation: Circ Res. 2011 Jan 21;108(2):219-34
references.pmid: 21252150
references.citation: Genes Cancer. 2010 Nov;1(11):1100-14
references.pmid: 21533017
references.citation: Int J Cancer. 2010 Jun 1;126(11):2575-83
references.pmid: 19839049
references.citation: Nat Rev Genet. 2011 Nov 18;12(12):861-74
references.pmid: 22094949
references.citation: Prog Mol Subcell Biol. 2010;50:1-20
references.pmid: 19841878
references.citation: Nat Med. 2011 Nov 20;17(12):1627-35
references.pmid: 22101765
references.citation: PLoS One. 2011;6(8):e23787
references.pmid: 21901135
references.citation: Am J Physiol Renal Physiol. 2011 Mar;300(3):F602-10
references.pmid: 21228106
references.citation: Nucleic Acids Res. 2009 Apr;37(5):1672-81
references.pmid: 19153141
references.citation: Cell. 2009 Jun 12;137(6):1005-17
references.pmid: 19524505
references.citation: Nat Cell Biol. 2008 May;10(5):593-601
references.pmid: 18376396
references.citation: J Biol Chem. 2011 Nov 25;286(47):40725-33
references.pmid: 21926171
references.citation: Oncogene. 2009 Jan 8;28(1):140-5
references.pmid: 18836483