title: Lipo-oligoarginine-based intracellular delivery.
abstract: Efficient cellular delivery, including plasma membrane permeability and intracellular metabolic stability, is a crucial factor determining the success of therapeutic agents. Cell-penetrating peptides (CPPs) have been widely used for the intracellular delivery of various bioactive molecules into cells to modify cellular functions. We have developed an improved CPP-based cellular delivery vector, named lipo-oligoarginine peptide (LOAP), by conjugating an oligoarginine peptide with a fatty acid moiety. The prepared LOAPs were further stabilized by introducing different combinations of D-Arg residues into the peptide backbone and were systematically evaluated for their membrane-penetrating properties and metabolic stabilities in cells.
authors.affiliation: Department of Radiology, Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX, USA.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Lee
authors.forename: Ching-Hsuan
authors.initials: CH
authors.lastname: Tung
mesh_terms: D001120:Arginine / Q000737:chemistry*; D016503:Drug Delivery Systems; D006801:Humans; D019169:Jurkat Cells; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
chemical_list: D001120:Arginine