title: CD106 identifies a subpopulation of mesenchymal stem cells with unique immunomodulatory properties.
abstract: Mesenchymal stem cells (MSCs) reside in almost all of the body tissues, where they undergo self-renewal and multi-lineage differentiation. MSCs derived from different tissues share many similarities but also show some differences in term of biological properties. We aim to search for significant differences among various sources of MSCs and to explore their implications in physiopathology and clinical translation. We compared the phenotype and biological properties among different MSCs isolated from human term placental chorionic villi (CV), umbilical cord (UC), adult bone marrow (BM) and adipose (AD). We found that CD106 (VCAM-1) was expressed highest on the CV-MSCs, moderately on BM-MSCs, lightly on UC-MSCs and absent on AD-MSCs. CV-MSCs also showed unique immune-associated gene expression and immunomodulation. We thus separated CD106(+)cells and CD106(-)cells from CV-MSCs and compared their biological activities. Both two subpopulations were capable of osteogenic and adipogenic differentiation while CD106(+)CV-MSCs were more effective to modulate T helper subsets but possessed decreased colony formation capacity. In addition, CD106(+)CV-MSCs expressed more cytokines than CD106(-)CV-MSCs. These data demonstrate that CD106 identifies a subpopulation of CV-MSCs with unique immunoregulatory activity and reveal a previously unrecognized mechanism underlying immunomodulation of MSCs.
authors.affiliation: The State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union of Medical College, Tianjin, China.
authors.forename: Zhou Xin
authors.initials: ZX
authors.lastname: Yang
authors.forename: Zhi-Bo
authors.initials: ZB
authors.lastname: Han
authors.forename: Yue Ru
authors.initials: YR
authors.lastname: Ji
authors.forename: You Wei
authors.initials: YW
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Liang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chi
authors.forename: Shao Guang
authors.initials: SG
authors.lastname: Yang
authors.forename: Li Na
authors.initials: LN
authors.lastname: Li
authors.forename: Wei Feng
authors.initials: WF
authors.lastname: Luo
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Li
authors.forename: Dan Dan
authors.initials: DD
authors.lastname: Chen
authors.forename: Wen Jing
authors.initials: WJ
authors.lastname: Du
authors.forename: Xiao Cang
authors.initials: XC
authors.lastname: Cao
authors.forename: Guang Sheng
authors.initials: GS
authors.lastname: Zhuo
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Zhong Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000276:immunology; D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D002823:Chorion / Q000166:cytology* / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006801:Humans; D056747:Immunomodulation; D059630:Mesenchymal Stem Cells / Q000145:classification / Q000166:cytology* / Q000276:immunology; D010011:Osteocytes / Q000166:cytology / Q000276:immunology; D011247:Pregnancy; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology; D014470:Umbilical Cord / Q000166:cytology / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D019010:Vascular Cell Adhesion Molecule-1
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