title: Microsatellite fragment analysis using the ABI PRISM ® 377 DNA sequencer.
abstract: The ABI PRISM (®) 377 DNA Sequencer is used for a variety of microsatellite-based research. The platform provides researchers with a cost-effective means for high-throughput genotyping, which can be further optimized by multiplexing microsatellite loci or by using a tail-labeling approach to screen large sets of markers. The goals of this chapter are to present a protocol for performing microsatellite-based analyses on the ABI 377 and to provide researchers with information on how to troubleshoot common issues associated with running the ABI 377 sequencers.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Renshaw
authors.forename: Melissa
authors.initials: M
authors.lastname: Giresi
authors.forename: J Orville
authors.initials: JO
authors.lastname: Adams
mesh_terms: D004247:DNA / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods
chemical_list: D004247:DNA