title: Dynamic evaluation of cell surface N-glycan expression via an electrogenerated chemiluminescence biosensor based on concanavalin A-integrating gold-nanoparticle-modified Ru(bpy)3(2+)-doped silica nanoprobe.
abstract: A sandwich electrogenerated chemiluminescence (ECL) biosensor was fabricated based on concanavalin A (Con A)-integrating gold-nanoparticle-modified Ru(bpy)3(2+)-doped silica nanoprobe (Au-RuSiO2 NPs) for in situ and dynamically evaluating cell surface N-glycan expression. Owing to the specific recognition of Con A with mannose and the core trimannoside fragment of N-glycan and the effective ECL amplification of Au-RuSiO2 NPs, the as-proposed biosensor exhibited excellent analytical performance toward the cytosensing of K562 cells with a wide detection linear range from 1.0 × 10(3) to 1.0 × 10(7) cells mL(-1) and a detection limit of 600 cells mL(-1). More importantly, the strategy was successfully applied to evaluate cell surface N-glycan expression under different external stimuli of inhibitors and enzyme. This biosensor is endowed with feasibility and reliability of generating sensitive insight into the majority of N-glycan expression on the cell surface. Furthermore, the biosensor was employed to dynamically profile cell surface N-glycan expression at different phases of cell growth in vitro. This biosensor is promising in studying and elucidating the N-glycan function in biological and physiological processes.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Tsinghua University , Beijing 100084, China.
authors.forename: Zhuhai
authors.initials: Z
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yangzhong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Jinghong
authors.initials: J
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques; D002462:Cell Membrane / Q000378:metabolism; D003208:Concanavalin A / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D049449:Luminescence; D015335:Molecular Probes / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis*; D012822:Silicon Dioxide / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D015335:Molecular Probes; D009942:Organometallic Compounds; D011134:Polysaccharides; C415768:tris(2,2'-bipyridyl)ruthenium(II); D003208:Concanavalin A; D006046:Gold; D012822:Silicon Dioxide