title: Oxysophocarpine induces anti-nociception and increases the expression of GABAAα1 receptors in mice.
abstract: Oxysophocarpine (OSC) is an alkaloid extracted from Siphocampylus verticillatus. The aim of this study was to investigate the anti-nociceptive effects of OSC through systemic and intracerebroventricular administration in mice. Moreover, to evaluate its effectiveness and mechanism of action, this study investigated whether OSC altered the expression of γ-aminobutyric acid type A α1 (GABAAα1) receptors in the central nervous system. Thermal and chemical behavioral models of nociception were used to assess the anti‑nociceptive action of OSC. The warm water tail-flick test, the hot‑plate test, acetic acid-induced abdominal constriction and formalin‑induced pain were used in mice. OSC was administered intraperitoneally (i.p.) or intracerebroventricularly (i.c.v.). Results showed that OSC (80 mg/kg, i.p.) significantly increased the tail withdrawal threshold with a peak effect of 25.46% maximal possible effect (MPE) at 60 min (P﹤0.01). Additionally, OSC (80 mg/kg) increased the positive staining of GABAAα1 receptors in cells. In conclusion, OSC administration is suggested to have anti-nociceptive effects on the central and peripheral nervous systems. The involvement of GABAA receptors in the anti-nociceptive activity of OSC is currently being investigated.
authors.affiliation: Department of Anesthesiology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Xu
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Li
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Hanxiang
authors.initials: H
authors.lastname: Ma
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Yu
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D029422:Campanulaceae / Q000737:chemistry; D002490:Central Nervous System / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D059408:Pain Management; D011963:Receptors, GABA-A / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000700:Analgesics; D011963:Receptors, GABA-A; C046246:oxysophocarpine; D000093842:Matrines