title: Knockdown of Bcl-3 inhibits cell growth and induces DNA damage in HTLV-1-infected cells.
abstract: Oncoprotein Bcl-3 is perceived as an unusual member of IκB family since it can both stimulate and suppress NF-κB activation. Aberrant Bcl-3 results in increased cell proliferation and survival, suggesting a contribution to malignant potential and elevated levels of Bcl-3 have been observed in many HTLV-1-infected T cell lines and ATL cells. To investigate the specific roles of Bcl-3 in HTLV-1-infected cells, we knocked down Bcl-3 expression using shRNA and then examined the consequences with regard to DNA damage and cell proliferation, as well as NF-κB activation. The HTLV-1 encoded protein Tax promotes Bcl-3 expression and nuclear translocation. In HTLV-1-infected cells, Bcl-3 knockdown obviously induced DNA damage. Cell growth and NF-κB activation were reduced in HTLV-1-infected or Tax positive cells when Bcl-3 expression was decreased. Together, our results revealed positive roles of Bcl-3 in DNA stabilization, growth and NF-κB activation in HTLV-1-infected cells.
authors.affiliation: Research Center for Immunology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: Cai
authors.initials: C
authors.lastname: Gao
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Jin-Heng
authors.initials: JH
authors.lastname: Wang
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Gao
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000081242:B-Cell Lymphoma 3 Protein; D049109:Cell Proliferation; D002478:Cells, Cultured; D004249:DNA Damage; D055785:Gene Knockdown Techniques; D016356:Gene Products, tax / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1; D006801:Humans; D019169:Jurkat Cells; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000821:virology; D016328:NF-kappa B / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000821:virology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000081242:B-Cell Lymphoma 3 Protein; C000631344:BCL3 protein, human; D016356:Gene Products, tax; D016328:NF-kappa B; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; C514970:tax protein, Human T-lymphotrophic virus 1