title: Versatility of enzymes catalyzing late steps in polyene 67-121C biosynthesis.
abstract: Actinoplanes caeruleus produces 67-121C, a heptaene macrolide modified with a D-mannosyl-D-mycosaminyl disaccharide. Draft genome sequencing revealed genes encoding mycosaminyltransferase, mycosamine synthase, a cytochrome P450 that modifies the macrolactone core, and the extending mannosyltransferase. Only the mycosamine synthase and P450 were active in the biosynthesis of amphotericins in Streptomyces nodosus, the amphotericin producer.
authors.affiliation: School of Biomolecular and Biomedical Science, Centre for Synthesis and Chemical Biology, University College Dublin, Dublin, Ireland.
authors.forename: Niamh
authors.initials: N
authors.lastname: Stephens
authors.forename: Bernard
authors.initials: B
authors.lastname: Rawlings
authors.forename: Patrick
authors.initials: P
authors.lastname: Caffrey
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D023281:Genomics; D006595:Hexosamines / Q000378:metabolism; D008848:Micromonosporaceae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011090:Polyenes / Q000378:metabolism*
chemical_list: D006595:Hexosamines; D011090:Polyenes; C014692:mycosamine; D003577:Cytochrome P-450 Enzyme System