title: Suppression of the lipid peroxidation process in the CNS reduces neurological expression of experimentally induced autoimmune encephalomyelitis.
abstract: OBJECTIVE
  Here we report the influence of malondialdehyde (MDA) as a measure of the lipid peroxidation process (LP), on multiple sclerosis (MS) pathogenesis and its neurological signs, during the treatment with aminoguanidine (AG) - a selective inducible nitric oxide synthase inhibitor and N-Acetyl cysteine (NAC) - an oxidative scavenger, in the experimental autoimmune encephalomyelitis (EAE), an animal model for studying MS.
  
  
  METHODS
  Encephalomyelitis induction by the subcutaneous injection of myelin basic protein of bovine type, dissolved in phosphate buffered saline (PBS) emulsified in equal volume of the complete Freund's adjuvant (CFA), was described in detail in our earlier published papers. Each of animals was randomly assigned to seven groups - control (PBS), EAE, CFA, EAE + AG, AG, EAE + NAC and NAC group. In each animal, the development of neurological signs of EAE was scored, these results were published earlier. MDA was evaluated in the central nervous system (CNS) structure - cerebellums and spinal cords.
  
  
  RESULTS
  The obtained results show that the AG and NAC treatment significantly reduces the MDA level in both examined tissues (p < 0.05) ameliorating at the same time EAE clinical signs (p < 0.05).
  
  
  CONCLUSIONS
  Taking together our present and earlier findings we conclude that LP may provoke and promote MS, while blocking of this process results in amelioration of the clinical onset and disease activity. These results may be useful as a new insight into mechanisms and potential targets for therapeutic strategies in MS.
authors.affiliation: Clinic of Neurology, University Clinical Centre of Nis, Nis, Serbia. srljub@gmail.com
authors.forename: Srdjan
authors.initials: S
authors.lastname: Ljubisavljevic
authors.forename: Ivana
authors.initials: I
authors.lastname: Stojanovic
authors.forename: Dusica
authors.initials: D
authors.lastname: Pavlovic
authors.forename: Maja
authors.initials: M
authors.lastname: Milojkovic
authors.forename: Dusan
authors.initials: D
authors.lastname: Sokolovic
authors.forename: Ivana
authors.initials: I
authors.lastname: Stevanovic
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Petrovic
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D015227:Lipid Peroxidation / Q000502:physiology*; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry / Q000378:metabolism / Q000473:pathology
chemical_list: D008315:Malondialdehyde