title: Comparative study of human aortic and mitral valve interstitial cell gene expression and cellular function.
abstract: Valve interstitial cells (VICs) are essential for valvular pathogenesis. However, the transcriptional profiles and cellular functions of human aortic VICs (hAVICs) and mitral VICs (hMVICs) have not been directly compared. We performed NimbleGen gene expression profiling analyses of hAVICs and hMVICs. Seventy-eight known genes were differentially expressed between hAVICs and hMVICs. Higher expression of NKX2-5, TBX15, OGN, OMD, and CDKN1C and lower expression of TBX5, MMP1, and PCDH10 were found in hAVICs compared to hMVICs. The differences in these genes, excepting OGN and OMD, remained in rheumatic VICs. We also compared cell proliferation, migration, and response to mineralization medium. hMVICs proliferated more quickly but showed more calcium deposition and alkaline phosphatase activity than hAVICs after culture in mineralization medium, indicating that hMVICs were more susceptible to in vitro calcification. Our findings reveal differences in the transcription profiles and cellular functions of hAVICs and hMVICs.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Shao
authors.forename: Xiangqing
authors.initials: X
authors.lastname: Kong
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001021:Aortic Valve / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D001024:Aortic Valve Stenosis / Q000378:metabolism / Q000473:pathology; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008943:Mitral Valve / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D008946:Mitral Valve Stenosis / Q000378:metabolism / Q000473:pathology; D012214:Rheumatic Heart Disease / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D018797:Cell Cycle Proteins; D014157:Transcription Factors; D000469:Alkaline Phosphatase; D002118:Calcium