title: Peach (Prunus persica) extract inhibits angiotensin II-induced signal transduction in vascular smooth muscle cells.
abstract: Angiotensin II (Ang II) is a vasoactive hormone that has been implicated in cardiovascular diseases. Here, the effect of peach, Prunus persica L. Batsch, pulp extract on Ang II-induced intracellular Ca(2+) mobilization, reactive oxygen species (ROS) production and signal transduction events in cultured vascular smooth muscle cells (VSMCs) was investigated. Pretreatment of peach ethyl acetate extract inhibited Ang II-induced intracellular Ca(2+) elevation in VSMCs. Furthermore, Ang II-induced ROS generation, essential for signal transduction events, was diminished by the peach ethyl acetate extract. The peach ethyl acetate extract also attenuated the Ang II-induced phosphorylation of epidermal growth factor receptor and myosin phosphatase target subunit 1, both of which are associated with atherosclerosis and hypertension. These results suggest that peach ethyl acetate extract may have clinical potential for preventing cardiovascular diseases by interfering with Ang II-induced intracellular Ca(2+) elevation, the generation of ROS, and then blocking signal transduction events.
authors.affiliation: Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University, Kimiidera 811-1, Wakayama City, Wakayama 641-0012, Japan. r-kono@wakayama-med.ac.jp
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kono
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okuno
authors.forename: Misa
authors.initials: M
authors.lastname: Nakamura
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Inada
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tokuda
authors.forename: Miki
authors.initials: M
authors.lastname: Yamashita
authors.forename: Ryu
authors.initials: R
authors.lastname: Hidaka
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Utsunomiya
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects*; D066246:ErbB Receptors / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism*; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000804:Angiotensin II; D066246:ErbB Receptors