title: Expression and significance of PTEN and miR-92 in hepatocellular carcinoma.
abstract: The aim of this study was to investigate the correlation between the expression levels of the tumor suppressor gene phosphatase and tensin homolog (PTEN) and the microRNA (miRNA) miR-92 in hepatocellular carcinoma (HCC) and paracancerous tissue. Immunohistochemistry [streptavidin-peroxidase (SP)] and quantitative reverse transcriptase-polymerase chain reaction (qRT‑PCR) were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues. The correlation between PTEN and miR-92 was analyzed. The expression of PTEN mRNA and protein was significantly lower in HCC compared with that in paracancerous tissues (P<0.05). miR-92 showed the opposite expression pattern (P<0.05). Additionally, the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other (r=-0.858, P<0.05). In conclusion, PTEN and miR-92 have different roles in the development of HCC. The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC.
authors.affiliation: School Hospital, Shandong University, Jinan, Shandong 250011, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Yueting
authors.initials: Y
authors.lastname: Zhu
authors.forename: Kai
authors.initials: K
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Fachang
authors.initials: F
authors.lastname: Yu
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human