title: Analyzing microsatellites using the QIAxcel system.
abstract: Microsatellites are ubiquitous throughout eukaryotic genomes and are useful in analyzing populations and genetic diversity. The QIAxcel system, an automated capillary electrophoresis device, allows the user to determine the size of microsatellite fragments, to discern allelic polymorphisms among individuals, and to differentiate homozygous and heterozygous individuals. This system provides comparable base pair resolution to more expensive systems at a relatively affordable cost.
authors.affiliation: Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Dean
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Wadl
authors.forename: Denita
authors.initials: D
authors.lastname: Hadziabdic
authors.forename: Xinwang
authors.initials: X
authors.lastname: Wang
authors.forename: Robert N
authors.initials: RN
authors.lastname: Trigiano
mesh_terms: D000180:Acrylic Resins; D000483:Alleles; D000818:Animals; D004247:DNA / Q000032:analysis / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D014644:Genetic Variation; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels; D004247:DNA