title: In vitro bioavailability of total selenium and selenium species from seafood.
abstract: In vitro bioavailability of total selenium and selenium species from different raw seafood has been assessed by using a simulated gastric and intestinal digestion/dialysis method. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to assess total selenium contents after a microwave assisted acid digestion, and also to quantify total selenium in the dialyzable and non-dialyzable fractions. Selenium speciation in the dialyzates was assessed by high performance liquid chromatography (HPLC) coupled with ICP-MS detection. Major Se species (selenium methionine and oxidized selenium methionine) from dialyzate were identified and characterized by HPLC coupled to mass spectrometry (HPLC-MS). Selenocystine was detected at low concentrations while Se-(Methyl)selenocysteine and inorganic selenium species (selenite and selenate) were not detected in the dialyzate. Low bioavailability percentages for total selenium (6.69±3.39 and 5.45±2.44% for fish and mollusk samples, respectively) were obtained. Similar bioavailability percentages was achieved for total selenium as a sum of selenium species (selenocystine plus oxidized selenium methionine and selenium methionine, mainly). HPLC-MS data confirmed SeMet oxidation during the in vitro procedure.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of A Coruña, Campus da Zapateira, s/n., 15071 A Coruña, Spain. jorge.moreda@udc.es
authors.forename: Jorge
authors.initials: J
authors.lastname: Moreda-Piñeiro
authors.forename: Antonio
authors.initials: A
authors.lastname: Moreda-Piñeiro
authors.forename: Vanessa
authors.initials: V
authors.lastname: Romarís-Hortas
authors.forename: Raquel
authors.initials: R
authors.lastname: Domínguez-González
authors.forename: Elia
authors.initials: E
authors.lastname: Alonso-Rodríguez
authors.forename: Purificación
authors.initials: P
authors.lastname: López-Mahía
authors.forename: Soledad
authors.initials: S
authors.lastname: Muniategui-Lorenzo
authors.forename: Darío
authors.initials: D
authors.lastname: Prada-Rodríguez
authors.forename: Pilar
authors.initials: P
authors.lastname: Bermejo-Barrera
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003553:Cystine / Q000031:analogs & derivatives* / Q000032:analysis; D005399:Fishes; D013058:Mass Spectrometry; D008974:Mollusca / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D012643:Selenium / Q000032:analysis*; D012645:Selenomethionine / Q000032:analysis*
chemical_list: D016566:Organoselenium Compounds; C009226:selenocystine; D003553:Cystine; D012645:Selenomethionine; D012643:Selenium