title: Enzymatic synthesis of glutathione using engineered Saccharomyces cerevisiae.
abstract: Two single gene cassettes, each containing one of the individual gene (γ-glutamylcysteine synthetase gene GSH1 or glutathione synthetase gene GSH2), were constructed under the control of alcohol dehydrogenase (ADH1) promoter and their respective native terminators. The recombinant plasmids constructed with Kan (r) or Hyg (r) as the selective markers and were transformed into Saccharomyces cerevisiae separately and jointly. Three engineered strains, GSH1-enhanced strain S.TS013/GSH1, GSH2-enhanced strain S.TS013/GSH2 and GSH1+GSH2 double-enhanced strain S.TS013/GSH1+GSH2, were constructed. Glutathione production using the recombinant strains to improve was then determined. By the cell dosage proportions of two engineered strains (S.TS013/GSH1, S.TS013/GSH2) and a two-stage reaction, GSH productivity increased by 84 and 59 % over that of the host strain and the S.TS013/GSH1+GSH2 strain, respectively.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Life Science and Food Engineering, Nanchang University, Nanchang, 330047, People's Republic of China. ahhblscjl@163.com
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Xie
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Cai
authors.forename: Cheng-shuai
authors.initials: CS
authors.lastname: Yang
authors.forename: Xue-hui
authors.initials: XH
authors.lastname: Duan
mesh_terms: D015870:Gene Expression; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D005981:Glutathione Synthase / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D005721:Glutamate-Cysteine Ligase; D005981:Glutathione Synthase; D005978:Glutathione