title: Gingival crevicularfluid osteoprotegerin levels in Indian population.
abstract: BACKGROUND
  Initial research indicated that higher concentration of osteoprotegerin (OPG) is associated with healthy periodontium (protective) and its concentration decreases as the periodontal disease progresses. However, till date, there are no studies to investigate the levels of OPG in gingival crevicular fluid (GCF) after the treatment of periodontitis. Hence, the present study was carried out to assess its concentration in GCF to find out their association if any, and to explore its possible use as a 'novel bone marker' of the host modulation of periodontal disease.
  
  
  METHODS
  Sixty-four subjects were divided into 4 groups (16 each), based on clinical attachment loss (CAL) and radiological parameters (bone loss); healthy (group I), gingivitis (group II), slight periodontitis (group III), and moderate-to-severe periodontitis (group IV). Moderate-to-severe periodontitis subjects, after nonsurgical periodontal treatment, (SRP) constituted group V. GCF samples were collected to estimate the levels of OPG using enzyme-linked immunosorbent assay (ELISA). The Kruskal-Wallis, Man-Whitney U test, and Wilcoxon signed-rank tests were carried out to compare OPG levels among groups. The Spearman rank correlation test was used to correlate OPG levels between the study groups and the clinical parameters; P < 0.05 was considered significant.
  
  
  RESULTS
  The highest mean OPG concentration in GCF was obtained for group I (162.47 ± 51.171 pg/ μL) and the least for group IV (10.92 ± 1.913 pg/μL), suggesting a negative correlation between OPG concentration and CAL. OPG concentrations in GCF after the treatment of group IV increased from 10.92 ± 1.913 pg/μL to 15.63 ± 4.679 pg/μL.
  
  
  CONCLUSIONS
  OPG concentration in GCF was inversely proportional to CAL and not an active progression factor for periodontal disease. Further, after the treatment of moderate-to-severe periodontitis subjects (group IV), OPG concentrations increased. Hence, it can be concluded that OPG could be considered as a 'novel bone marker' the host modulation of periodontal disease.
authors.affiliation: Department of Periodontics, Government Dental College and RI, Bengaluru, Karnataka, India.
authors.forename: Purnima
authors.initials: P
authors.lastname: Bandari
authors.forename: M V Ramachandra
authors.initials: MV
authors.lastname: Prasad
authors.forename: Arun
authors.initials: A
authors.lastname: Maradi
authors.forename: A R
authors.initials: AR
authors.lastname: Pradeep
authors.forename: A
authors.initials: A
authors.lastname: Mallika
authors.forename: Dileep
authors.initials: D
authors.lastname: Sharma
keywords: Gingival crevicular fluid; Osteoprotegerin; periodontal disease
references.citation: J Dent Res. 2004 Feb;83(2):166-9
references.pmid: 14742657
references.citation: Cell. 1997 Apr 18;89(2):309-19
references.pmid: 9108485
references.citation: Curr Pharm Des. 2001 May;7(8):613-35
references.pmid: 11375772
references.citation: Clin Prev Dent. 1986 Jan-Feb;8(1):3-6
references.pmid: 3485495
references.citation: Physiol Rev. 1986 Oct;66(4):855-86
references.pmid: 3532144
references.citation: Inflammation. 2010 Jun;33(3):200-6
references.pmid: 20033478
references.citation: J Periodontol. 2006 Oct;77(10):1674-80
references.pmid: 17032109
references.citation: J Dent Res. 2004 Mar;83(3):241-4
references.pmid: 14981127
references.citation: Ann Periodontol. 1999 Dec;4(1):1-6
references.pmid: 10863370
references.citation: Infect Immun. 2005 Jan;73(1):342-51
references.pmid: 15618171
references.citation: Arthritis Res Ther. 2003;5(5):239-45
references.pmid: 12932284
references.citation: J Periodontal Res. 2006 Aug;41(4):354-60
references.pmid: 16827732
references.citation: Endocrinology. 1998 Mar;139(3):1329-37
references.pmid: 9492069
references.citation: J Dent Res. 2010 Dec;89(12):1349-63
references.pmid: 20739705
references.citation: J Periodontol. 2005 Nov;76(11 Suppl):2033-41
references.pmid: 16277573
references.citation: Annu Rev Immunol. 2002;20:795-823
references.pmid: 11861618
references.citation: Rheumatology (Oxford). 2003 Jan;42(1):123-34
references.pmid: 12509625
references.citation: J Periodontal Res. 1981 Jan;16(1):13-9
references.pmid: 6453968