title: Alum Directly Modulates Murine B Lymphocytes to Produce IgG1 Isotype.
abstract: Aluminum hydroxide (alum) is the most widely used adjuvant in human vaccines. Nevertheless, it is virtually unknown whether alum acts on B cells. In the present study, we explored the direct effect of alum on Ig expression by murine B cells in vitro. LPS-activated mouse spleen B cells were cultured with alum, and the level of isotype-specific Ig secretion, IgG1 secreting cell numbers, and Ig germ-line transcripts (GLT) were measured using ELISA, ELISPOT, and RT-PCR, respectively. Alum consistently enhanced total IgG1 production, numbers of IgG1 secreting cells, and GLTγ1 expression. These results demonstrate that alum can directly cause IgG1 isotype switching leading to IgG1 production.
authors.affiliation: Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Jin
authors.forename: Sun-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Hye-Ju
authors.initials: HJ
authors.lastname: Han
authors.forename: Young-Saeng
authors.initials: YS
authors.lastname: Jang
authors.forename: Goo-Young
authors.initials: GY
authors.lastname: Seo
authors.forename: Pyeung-Hyeun
authors.initials: PH
authors.lastname: Kim
keywords: Alum; B lymphocyte; IgG1; Isotype switch
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