title: Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined Essential 8™ Medium conditions.
abstract: Human-induced pluripotent stem cells (iPSCs) are an important potential source of cells for regenerative medicine due to their inherent ability to differentiate into all cell types of the three germ layers. Generation of iPSCs with a non-integrating reprogramming method and in culture conditions that are completely absent of animal proteins will be ideal for such regenerative and cell therapy applications. Here we describe a method to generate non-integrating iPSCs using the Episomal iPSC Reprogramming Vectors.
authors.affiliation: Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Fontes
authors.forename: Chad C
authors.initials: CC
authors.lastname: Macarthur
authors.forename: Pauline T
authors.initials: PT
authors.lastname: Lieu
authors.forename: Mohan C
authors.initials: MC
authors.lastname: Vemuri
mesh_terms: D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation; D002478:Cells, Cultured; D003470:Culture Media; D018274:Electroporation; D005347:Fibroblasts / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection; D019096:Vitronectin / Q000737:chemistry
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D014157:Transcription Factors; D019096:Vitronectin