title: Molecular detection of Ehrlichia canis in dogs from the Pantanal of Mato Grosso State, Brazil.
abstract: The present study evaluated the presence of Ehrlichia DNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsb gene. Risk factors for infection in dogs were also evaluated. Forty-eight (15%, 95% CI: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% CI: 10.4-22.2%) from the urban area and 23 (14.4%, 95% CI: 9.3-20.8%) from the rural area (P > 0.05). Partial DNA sequence obtained from PCR products of 18 samples from the urban area and 16 samples from the rural area were 100% identical to E. canis from Brazil and the USA. This study reports the first E. canis molecular detection in dogs from the northern Pantanal region.
authors.forename: Luana Gabriela Ferreira Dos
authors.initials: LG
authors.lastname: Santos
authors.forename: Andréia Lima Tomé
authors.initials: AL
authors.lastname: Melo
authors.forename: Jonas
authors.initials: J
authors.lastname: Moraes-Filho
authors.forename: Rute
authors.initials: R
authors.lastname: Witter
authors.forename: Marcelo Bahia
authors.initials: MB
authors.lastname: Labruna
authors.forename: Daniel Moura de
authors.initials: DM
authors.lastname: Aguiar
mesh_terms: D000818:Animals; D001938:Brazil; D004269:DNA, Bacterial / Q000097:blood*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics*; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D025202:Molecular Diagnostic Techniques
chemical_list: D004269:DNA, Bacterial