title: [Effect of RelB on HIV-1 Vpr-mediated transcription activation and cell G2/M arrest].
abstract: Vpr, an auxiliary protein of HIV-1(Human immunodeficiency virus type 1), exerts important functions to promote viral replication and AIDS progression. In this study, we performed a yeast two-hybrid screening assay using human cDNA library to further investigate the molecular mechanism of various functions of Vpr RelB, a key protein in NF-kappaB signaling pathway, was identified as a Vpr interaction protein by co-immunoprecipitation. Further investigations indicated that RelB not only promoted the Vpr-mediated activation of NF-kappaB reporter gene, but also enhanced the transactivation of HIV LTR. Moreover, the results showed that RelB promoted Vpr-induced cell cycle G2/M arrest. Collectively, these results indicated that RelB might interact with Vpr and regulate its transcriptional activation and cell cycle arrest.
authors.affiliation: Key Laboratory of Molecular Microbiology and Biotechnology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Rui-Kang
authors.initials: RK
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Lin
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
authors.forename: Yun-Qi
authors.initials: YQ
authors.lastname: Geng
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Qiao
mesh_terms: D059447:Cell Cycle Checkpoints; D002455:Cell Division; D016195:G2 Phase; D016325:HIV Long Terminal Repeat; D006367:HeLa Cells; D006801:Humans; D016328:NF-kappa B / Q000235:genetics; D051841:Transcription Factor RelB / Q000502:physiology*; D015533:Transcriptional Activation; D054325:vpr Gene Products, Human Immunodeficiency Virus / Q000502:physiology*
chemical_list: D016328:NF-kappa B; C497593:RELB protein, human; D054325:vpr Gene Products, Human Immunodeficiency Virus; C514912:vpr protein, Human immunodeficiency virus 1; D051841:Transcription Factor RelB