title: Osteogenic differentiation of human bone marrow mesenchymal stem cells in hydrogel containing nacre powder.
abstract: Nacre (or mother of pearl) can facilitate bone cell differentiation and can speed up their mineralization. Here we report on the capability of nacre to induce differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) and the production of extracellular matrix. hBM-MSCs were encapsulated in an alginate hydrogel containing different concentrations of powdered nacre and cultured in the same environment until Day 28. Analysis of osteogenic gene expression, histochemistry, second harmonic generation (SHG) microscopy, and Raman scattering spectroscopy were used to characterize the synthesis of the extracellular matrix. In the presence of nacre powder, a significant increase in matrix synthesis from D21 in comparison with pure alginate was observed. Histochemistry revealed the formation of a new tissue composed of collagen fibers in the presence of nacre (immunostaining and SHG), and hydroxyapatite crystals (Raman) in the alginate beads. These results suggest that nacre is efficient in hBM-MSCs differentiation, extracellular matrix production and mineralization in alginate 3D biomaterials.
authors.affiliation: UMR 7365 CNRS-Université de Lorraine, Faculté de médecine, 9 avenue de la forêt de Haye, 54505, Vandoeuvre-les-Nancy, France.
authors.forename: Alicia
authors.initials: A
authors.lastname: Flausse
authors.forename: Christel
authors.initials: C
authors.lastname: Henrionnet
authors.forename: Manuel
authors.initials: M
authors.lastname: Dossot
authors.forename: Dominique
authors.initials: D
authors.lastname: Dumas
authors.forename: Sébastien
authors.initials: S
authors.lastname: Hupont
authors.forename: Astrid
authors.initials: A
authors.lastname: Pinzano
authors.forename: Didier
authors.initials: D
authors.lastname: Mainard
authors.forename: Laurent
authors.initials: L
authors.lastname: Galois
authors.forename: Jacques
authors.initials: J
authors.lastname: Magdalou
authors.forename: Evelyne
authors.initials: E
authors.lastname: Lopez
authors.forename: Pierre
authors.initials: P
authors.lastname: Gillet
authors.forename: Marthe
authors.initials: M
authors.lastname: Rousseau
mesh_terms: D000368:Aged; D000464:Alginates / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D024121:Collagen Type X / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D036641:Microscopy, Fluorescence, Multiphoton; D008863:Microspheres; D008875:Middle Aged; D060734:Nacre / Q000494:pharmacology*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D011208:Powders; D013059:Spectrum Analysis, Raman
chemical_list: D000464:Alginates; D024121:Collagen Type X; D050659:Core Binding Factor Alpha 1 Subunit; D006603:Hexuronic Acids; D060734:Nacre; D011208:Powders; D015675:Osteocalcin; D053495:Osteopontin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid
keywords: ECM; bone tissue engineering; hBM-MSCs; nacre; osteogenic differentiation