title: Demonstration of estrogen receptor α protein in glutamatergic (vesicular glutamate transporter 2 immunoreactive) neurons of the female rat hypothalamus and amygdala using double-label immunocytochemistry.
abstract: By means of double-label immunocytochemistry, authors studied the presence of estrogen receptor α (ER-α) protein in vesicular glutamate transporter 2 (VGluT2) protein-immunoreactive neurons in the female rat hypothalamus and amygdala. They examined colocalization of the 2 immunoreactive proteins in structures in which they found a significant overlap in the localization of the distribution of ER-α- and VGluT2-immunopositive nerve cells, namely in the medial preoptic area, the ventral subdivision of the ventromedial hypothalamic nucleus, and the medial amygdaloid nucleus. In the medial preoptic area, only 2.74 % of ER-α-immunoreactive neurons were VGluT2 positive, and conversely, 5 % of VGluT2-immunoreactive neurons contained ER-α immunofluorescent labeling. Highest degree of colocalization was detected in the ventral subdivision of the ventromedial hypothalamic nucleus, where 22.81 % of the ER-α-immunopositive neurons were VGluT2 immunoreactive and 37.14 % of the VGluT2-immunolabeled neurons contained ER-α-positive nucleus. In the medial amygdaloid nucleus, 15.38 % of the ER-α and 18.1 % of the VGluT2-immunoreactive neurons were double labeled. The colocalizations suggest that glutamatergic (VGluT2 protein immunoreactive) neurons are involved in the mediation of the action of estrogen on the rat brain.
authors.affiliation: Department of Human Morphology and Developmental Biology, Semmelweis University, Tűzoltó utca 58, 1094 Budapest, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Kiss
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Csaba
authors.forename: Agnes
authors.initials: A
authors.lastname: Csáki
authors.forename: Béla
authors.initials: B
authors.lastname: Halász
mesh_terms: D000679:Amygdala / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; C493475:Slc17a6 protein, rat; D050599:Vesicular Glutamate Transport Protein 2
references.citation: Brain Res Brain Res Rev. 2001 Nov;37(1-3):153-61
references.pmid: 11744083
references.citation: Neuroendocrinology. 1995 Mar;61(3):213-25
references.pmid: 7898626
references.citation: Science. 1990 Nov 30;250(4985):1276-8
references.pmid: 1978759
references.citation: Neuroscience. 2007 Apr 25;146(1):160-9
references.pmid: 17317017
references.citation: J Neuroendocrinol. 2004 Jan;16(1):26-31
references.pmid: 14962072
references.citation: J Comp Neurol. 2003 Oct 6;465(1):136-44
references.pmid: 12926021
references.citation: Endocrinology. 2003 Jan;144(1):299-305
references.pmid: 12488358
references.citation: Eur J Neurosci. 2005 Apr;21(8):2111-9
references.pmid: 15869507
references.citation: Endocrinology. 2004 Sep;145(9):4018-21
references.pmid: 15205380
references.citation: Brain Res. 1999 Dec 4;849(1-2):253-74
references.pmid: 10592312
references.citation: Endocrinology. 1997 Feb;138(2):778-89
references.pmid: 9003015
references.citation: J Neuroendocrinol. 2002 Feb;14(2):85-94
references.pmid: 11849367
references.citation: J Comp Neurol. 2004 May 24;473(2):270-91
references.pmid: 15101093
references.citation: Endocr Rev. 1999 Jun;20(3):279-307
references.pmid: 10368772
references.citation: Endocr Rev. 1997 Oct;18(5):678-700
references.pmid: 9331548
references.citation: Recent Prog Horm Res. 2002;57:357-84
references.pmid: 12017552
references.citation: Brain Res Bull. 2007 Nov 1;74(6):397-405
references.pmid: 17920448
references.citation: J Chem Neuroanat. 2009 Nov;38(3):185-96
references.pmid: 19505570
references.citation: Brain Res Mol Brain Res. 2002 Jul 15;104(1):47-54
references.pmid: 12117550
references.citation: J Comp Neurol. 1997 Dec 1;388(4):507-25
references.pmid: 9388012
references.citation: Endocrinology. 2003 Feb;144(2):662-70
references.pmid: 12538629