title: apoB-independent enzyme immunoassay for lipoprotein(a) by capture on immobilized lectin (jacalin).
abstract: Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).
authors.affiliation: Department of Biochemistry, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Sreekumar
authors.forename: Geetha
authors.initials: G
authors.lastname: Mandagini
authors.forename: Sabarinath P
authors.initials: SP
authors.lastname: Subramanian
authors.forename: Appukuttan P
authors.initials: AP
authors.lastname: Sankunni
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000737:chemistry; D001054:Apolipoproteins A / Q000737:chemistry; D001055:Apolipoproteins B / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood; D017270:Lipoprotein(a) / Q000097:blood*; D009195:Peroxidase / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry*
chemical_list: D000906:Antibodies; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D055767:Immobilized Proteins; D007070:Immunoglobulin A; D017270:Lipoprotein(a); D037121:Plant Lectins; C043696:jacalin; D009195:Peroxidase