title: The role of thioredoxin h in protein metabolism during wheat (Triticum aestivum L.) seed germination.
abstract: Thioredoxin h can regulate the redox environment in the cell and play an important role in the germination of cereals. In the present study, the thioredoxin s antisense transgenic wheat with down-regulation of thioredoxin h was used to study the role of thioredoxin h in protein metabolism during germination of wheat seeds, and to explore the mechanism of the thioredoxin s antisense transgenic wheat seeds having high resistance to pre-harvest sprouting. The qRT-PCR results showed that the expression of protein disulfide isomerase in the thioredoxin s antisense transgenic wheat was up-regulated, which induced easily forming glutenin macropolymers and the resistance of storage proteins to degradation. The expression of serine protease inhibitor was also up-regulated in transgenic wheat, which might be responsible for the decreased activity of thiocalsin during the germination. The expression of WRKY6 in transgenic wheat was down-regulated, which was consistent with the decreased activity of glutamine oxoglutarate aminotransferase. In transgenic wheat, the activities of glutamate dehydrogenase, glutamic pyruvic transaminase and glutamic oxaloacetic transaminase were down-regulated, indicating that the metabolism of amino acid was lower than that in wild-type wheat during seed germination. A putative model for the role of thioredoxin h in protein metabolism during wheat seed germination was proposed and discussed.
authors.affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, 450002, China; College of Life Sciences, Henan Agricultural University, Zhengzhou, China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, China.
authors.forename: Shaoxin
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Xu
authors.affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Jiangping
authors.initials: J
authors.lastname: Ren
authors.affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Niu
authors.affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address: xmzxyj@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D018506:Gene Expression Regulation, Plant; D018525:Germination; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000378:metabolism*; D054480:Thioredoxin h / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D054480:Thioredoxin h
keywords: Protein metabolism; Seed germination; Thioredoxin h; Wheat