title: Epithelial to mesenchymal transition and the generation of stem-like cells in pancreatic cancer.
abstract: An epithelial-to-mesenchymal transition (EMT) is thought to be an important process in the acquisition of capabilities required for metastasis. Until recently, studies of EMT involved mostly in vitro assays and transplantation experiments of cancer cells that overexpressed known EMT drivers. While valuable, these studies do not allow us to conclude if an EMT sustained under "physiologic conditions" within the tumor microenvironment leads to the myriad changes in phenotype observed in vitro. Here we review our recently published work using a lineage labeled genetically engineered mouse model of pancreatic ductal adenocarcinoma to characterize cells that have sustained an EMT in vivo.
authors.affiliation: Gastroenterology Division and Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. arhim@mail.med.upenn.edu
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Rhim
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D010179:Pancreas / Q000166:cytology / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000473:pathology*
references.citation: Cancer Res. 2006 Jan 15;66(2):944-50
references.pmid: 16424029
references.citation: Science. 2009 Jun 12;324(5933):1457-61
references.pmid: 19460966
references.citation: Cancer Cell. 2005 May;7(5):469-83
references.pmid: 15894267
references.citation: Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14884-9
references.pmid: 19706487
references.citation: Nat Cell Biol. 2000 Feb;2(2):76-83
references.pmid: 10655586
references.citation: Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):75-80
references.pmid: 20018761
references.citation: Cancer Res. 2007 Feb 1;67(3):1030-7
references.pmid: 17283135
references.citation: Development. 2012 Oct;139(19):3471-86
references.pmid: 22949611
references.citation: Oncogene. 2005 Mar 31;24(14):2375-85
references.pmid: 15674322
references.citation: Cell. 2008 May 16;133(4):704-15
references.pmid: 18485877
references.citation: Cell. 2009 Nov 25;139(5):871-90
references.pmid: 19945376
references.citation: Oncogene. 2010 Aug 26;29(34):4741-51
references.pmid: 20531305
references.citation: Cancer Res. 2008 Jan 15;68(2):537-44
references.pmid: 18199550
references.citation: Cancer Cell. 2006 Dec;10(6):515-27
references.pmid: 17157791
references.citation: Cell. 2012 Jan 20;148(1-2):349-61
references.pmid: 22265420
references.citation: Cell. 2004 Jun 25;117(7):927-39
references.pmid: 15210113
references.citation: Carcinogenesis. 2008 Jun;29(6):1092-5
references.pmid: 18453542
references.citation: Nat Rev Cancer. 2009 Apr;9(4):265-73
references.pmid: 19262571