title: RNA interference-mediated knockdown of DGAT1 decreases triglyceride content of bovine mammary epithelial cell line.
abstract: Diacylglyceroltransferase-1 (DGAT1) expresses in nearly all tissues, including the mammary gland. Mice lacking DGAT1 exhibit decreased triglyceride content in mammary tissue, and are resistant to diet-induced obesity and diabetes mellitus. Thus, DGAT1 has received considerable attention. In the present study, the function of DGAT1 was examined by liposome mediated RNA interference (RNAi) to knockdown the expression of endogenous DGAT1 expression in bovine mammary epithelial cells (BMEC) and the changes of the biological functions of cells were analyzed. The mRNA and protein levels, intracellular triglyceride (TG) content, and total protein of BMECs were analyzed by real-time PCR, Western blot, TG kit, and ultraviolet spectrophotometer, respectively, before and after RNAi treatment. The results indicated that knockdown of DGAT1 expression significantly reduced TG content in BMECs. This study further confirmed the importance of DGAT1 in triglyceride synthesis in bovine mammary tissue.
authors.affiliation: College of Animal Science, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, Jilin Province, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Lu
authors.forename: Runjun
authors.initials: R
authors.lastname: Yang
authors.forename: Binglei
authors.initials: B
authors.lastname: Shen
authors.forename: Hassan
authors.initials: H
authors.lastname: Osman
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shouqing
authors.initials: S
authors.lastname: Yan
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D017931:DNA Primers; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism; D055785:Gene Knockdown Techniques; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D014280:Triglycerides; D051048:Diacylglycerol O-Acyltransferase
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