title: Anxiolytic property of hydro-alcohol extract of Lactuca sativa and its effect on behavioral activities of mice.
abstract: Lactuca sativa, belonging to the Asteraceae family, is a leafy vegetable known for its medicinal properties. This study aimed to understand the mechanism of Lactuca sativa extract with respect to pharmacological action.We investigated the anxiolytic effects of hydro-alcoholic extract of leaves of Lactuca sativa on mice. The behavioral tests performed on mice models to assess anti-anxiety properties were: open field test (OFT), elevated plus maze test (EPM), elevated T maze test, and marble burying test. Increased locomotor activity and time spent in the "open-arm" were observed in extract fed group. Malondialdehyde (MDA) and nitrite levels were decreased, catalase and glutathione levels were increased in Lactuca sativa treated mice. The data obtained in the present study suggests that the extract of Lactuca sativa can afford significant protection against anxiolytic activity.
authors.affiliation: Applied Nutrition Discipline, Defence Food Research Laboratory, Mysore 570011, India.
authors.forename: Singapura Nagesh
authors.initials: SN
authors.lastname: Harsha
authors.forename: Kandangath Raghavan
authors.initials: KR
authors.lastname: Anilakumar
keywords: Lactuca sativa; anxiety; elevated plus maze test (EPM); malondialdehyde (MDA); nitrite; open field test (OFT)
references.citation: Behav Brain Res. 1998 Aug;94(2):249-54
references.pmid: 9722276
references.citation: Methods Enzymol. 1978;52:302-10
references.pmid: 672633
references.citation: J Ethnopharmacol. 2005 Oct 31;102(1):23-31
references.pmid: 16054316
references.citation: Arch Biochem Biophys. 1959 May;82(1):70-7
references.pmid: 13650640
references.citation: Diabetes. 1991 Apr;40(4):405-12
references.pmid: 2010041
references.citation: Arch Pharm Res. 2002 Oct;25(5):643-6
references.pmid: 12433198
references.citation: Biol Pharm Bull. 2006 Mar;29(3):451-4
references.pmid: 16508144
references.citation: J Biol Chem. 2000 Sep 01;275(35):26877-84
references.pmid: 10858433
references.citation: Free Radic Biol Med. 1999 May;26(9-10):1231-7
references.pmid: 10381194
references.citation: Int J Biochem Cell Biol. 2002 May;34(5):439-58
references.pmid: 11906817
references.citation: Anal Biochem. 1970 Mar;34:30-8
references.pmid: 5440916
references.citation: J Agric Food Chem. 2008 Feb 27;56(4):1415-22
references.pmid: 18220345
references.citation: J Agric Food Chem. 2002 Nov 6;50(23):6910-6
references.pmid: 12405796
references.citation: Behav Brain Res. 2003 Dec 17;147(1-2):185-92
references.pmid: 14659584
references.citation: Indian J Exp Biol. 1997 Jun;35(6):565-75
references.pmid: 9357158
references.citation: Behav Brain Res. 2002 Oct 17;136(1):1-12
references.pmid: 12385785
references.citation: Brain Res. 2002 Jun 28;942(1-2):71-8
references.pmid: 12031854
references.citation: Free Radic Res Commun. 1993;19(3):141-58
references.pmid: 8244084
references.citation: J Agric Food Chem. 2007 Dec 12;55(25):10366-72
references.pmid: 17990849
references.citation: J Ethnopharmacol. 2004 Jun;92(2-3):325-9
references.pmid: 15138019
references.citation: Pharmacol Biochem Behav. 1994 Nov;49(3):549-54
references.pmid: 7862706
references.citation: Eur J Biochem. 2002 Apr;269(8):1996-2002
references.pmid: 11985575
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Int J Neuropsychopharmacol. 2008 Sep;11(6):851-76
references.pmid: 18205981