title: Kinetics of olfactory responses might largely depend on the odorant-receptor interaction and the odorant deactivation postulated for flux detectors.
abstract: Experimental data together with modeling of pheromone perireceptor and receptor events in moths (Bombyx mori, Antheraea polyphemus) suggest that the kinetics of olfactory receptor potentials largely depend on the association of the odorant with the neuronal receptor molecules and the deactivation of the odorant accumulated around the receptor neuron. The first process could be responsible for the reaction times (mean about 400 ms) of the nerve impulses at threshold. The second process has been postulated for flux detectors such as olfactory sensilla of moths. The odorant deactivation could involve a modification of the pheromone-binding protein (PBP) that "locks" the pheromone inside the inner binding cavity of the protein. The model combines seemingly contradictory functions of the PBP such as pheromone transport, protection of the pheromone from enzymatic degradation, pheromone deactivation, and pheromone-receptor interaction. Model calculations reveal a density of at least 6,000 receptor molecules per µm(2) of neuronal membrane. The volatile decanoyl-thio-1,1,1-trifluoropropanone specifically blocks pheromone receptor neurons, probably when bound to the PBP and by competitive binding to the receptor molecules. The shallow dose-response curve of the receptor potential and altered response properties observed with pheromone derivatives or after adaptation may indicate shortened opening of ion channels.
authors.affiliation: Max-Planck-Institut fuer Verhaltensphysiologie/Ornithologie, Seewiesen, 82319, Starnberg, Germany, kaissling@orn.mpg.de.
authors.forename: Karl-Ernst
authors.initials: KE
authors.lastname: Kaissling
mesh_terms: D000818:Animals; D007700:Kinetics; D008954:Models, Biological; D009036:Moths; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D018035:Receptors, Odorant / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D012903:Smell / Q000502:physiology*
chemical_list: D018035:Receptors, Odorant
references.citation: PLoS Comput Biol. 2009 Mar;5(3):e1000321
references.pmid: 19300479
references.citation: Chem Biol. 2000 Feb;7(2):143-51
references.pmid: 10662696
references.citation: Cell Tissue Res. 1998 Oct;294(1):179-85
references.pmid: 9724468
references.citation: Ann N Y Acad Sci. 1998 Nov 30;855:316-9
references.pmid: 10049224
references.citation: Nature. 2007 Nov 8;450(7167):289-93
references.pmid: 17943085
references.citation: Cell Tissue Res. 2001 Mar;303(3):433-46
references.pmid: 11320659
references.citation: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2009 Oct;195(10):895-922
references.pmid: 19697043
references.citation: Naturwissenschaften. 1970 Jan;57(1):23-8
references.pmid: 5417282
references.citation: Genet Mol Res. 2011 Dec 08;10(4):3056-69
references.pmid: 22180039
references.citation: Chem Senses. 2011 May;36(4):335-44
references.pmid: 21220518
references.citation: Front Cell Neurosci. 2009 Sep 09;3:10
references.pmid: 19826623
references.citation: Chem Senses. 2000 Feb;25(1):31-7
references.pmid: 10667991
references.citation: J Exp Biol. 2003 May;206(Pt 9):1575-88
references.pmid: 12654896
references.citation: Chem Senses. 1998 Feb;23(1):99-111
references.pmid: 9530975
references.citation: J Biomol NMR. 2001 Jan;19(1):79-80
references.pmid: 11246858
references.citation: Chem Senses. 2001 Feb;26(2):125-50
references.pmid: 11238244
references.citation: Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14075-9
references.pmid: 16172410
references.citation: Chemphyschem. 2008 Dec 22;9(18):2785-93
references.pmid: 19025731
references.citation: Biochemistry. 1995 Jul 11;34(27):8726-32
references.pmid: 7612612
references.citation: J Comp Physiol B. 2003 Sep;173(7):565-73
references.pmid: 12879348
references.citation: Chem Biol. 2003 Apr;10(4):331-40
references.pmid: 12725861
references.citation: J Biol Chem. 1999 Oct 22;274(43):30950-6
references.pmid: 10521490
references.citation: Nature. 1981 Sep 10-16;293(5828):161-3
references.pmid: 18074618
references.citation: J Neurophysiol. 2012 Oct;108(7):2051-9
references.pmid: 22815404
references.citation: Science. 2005 Jun 24;308(5730):1931-4
references.pmid: 15976304
references.citation: Chem Commun (Camb). 2009 Oct 14;(38):5725-7
references.pmid: 19774249
references.citation: J Comp Physiol A. 2000 Mar;186(3):315-23
references.pmid: 10757247
references.citation: Cell Mol Neurobiol. 1984 Dec;4(4):385-96
references.pmid: 6532523
references.citation: J Gen Physiol. 1975 Dec;66(6):823-36
references.pmid: 1194890
references.citation: Biochemistry. 2000 Aug 1;39(30):8953-62
references.pmid: 10913308
references.citation: Proc Natl Acad Sci U S A. 1985 Dec;82(24):8827-31
references.pmid: 3001718
references.citation: J Neurosci. 2008 Apr 30;28(18):4745-55
references.pmid: 18448651
references.citation: Science. 2005 Mar 11;307(5715):1638-42
references.pmid: 15692016
references.citation: Cold Spring Harb Symp Quant Biol. 1965;30:517-27
references.pmid: 5219500
references.citation: Microsc Res Tech. 1999 Dec 15;47(6):416-27
references.pmid: 10607381
references.citation: Proc Natl Acad Sci U S A. 2011 May 24;108(21):8821-5
references.pmid: 21555561
references.citation: J Mol Biol. 2009 Jun 12;389(3):529-45
references.pmid: 19371749
references.citation: Chem Senses. 2008 Mar;33(3):291-9
references.pmid: 18209018
references.citation: Structure. 2006 Oct;14(10):1577-86
references.pmid: 17027506
references.citation: Nature. 1982 Feb 18;295(5850):601-3
references.pmid: 6276777
references.citation: Neuropharmacology. 2012 Apr;62(5-6):2086-95
references.pmid: 22269900
references.citation: Chem Senses. 1996 Dec;21(6):719-27
references.pmid: 8985600
references.citation: Biochem Biophys Res Commun. 2008 Aug 8;372(4):559-64
references.pmid: 18503757
references.citation: Neuron. 2005 Jan 20;45(2):193-200
references.pmid: 15664171
references.citation: Pest Manag Sci. 2004 Jul;60(7):719-26
references.pmid: 15260305
references.citation: PLoS One. 2011;6(12):e29147
references.pmid: 22216190
references.citation: Insect Biochem Mol Biol. 2008 Aug;38(8):770-80
references.pmid: 18625400
references.citation: Biochemistry. 1994 Apr 26;33(16):4812-9
references.pmid: 8161541
references.citation: Nature. 2008 Apr 24;452(7190):1007-11
references.pmid: 18408711
references.citation: PLoS One. 2012;7(5):e36784
references.pmid: 22590607
references.citation: Biosystems. 2007 May-Jun;89(1-3):101-9
references.pmid: 17284344
references.citation: J Neurosci. 2011 May 25;31(21):7891-9
references.pmid: 21613503
references.citation: Ann N Y Acad Sci. 1987;510:104-12
references.pmid: 3324874
references.citation: Annu Rev Neurosci. 1986;9:121-45
references.pmid: 3518584
references.citation: Nat Rev Neurosci. 2010 Mar;11(3):188-200
references.pmid: 20145624
references.citation: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2003 Mar;189(3):221-30
references.pmid: 12664098
references.citation: Tissue Cell. 1984;16(5):705-17
references.pmid: 6515640
references.citation: PLoS One. 2012;7(9):e44190
references.pmid: 22957053
references.citation: Eur J Biochem. 1995 Sep 15;232(3):706-11
references.pmid: 7588707
references.citation: J Chem Ecol. 2010 Mar;36(3):245-8
references.pmid: 20191395
references.citation: Int J Biol Sci. 2009 Dec 03;5(7):745-57
references.pmid: 20011135
references.citation: J Mol Biol. 2011 May 20;408(5):922-31
references.pmid: 21396939
references.citation: Naturwissenschaften. 2002 Nov;89(11):515-8
references.pmid: 12451455
references.citation: Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14374-9
references.pmid: 11724947
references.citation: J Biol Chem. 1997 Jun 6;272(23):14792-9
references.pmid: 9169446
references.citation: Curr Opin Neurobiol. 2009 Jun;19(3):284-92
references.pmid: 19660933
references.citation: PLoS One. 2011 Mar 02;6(3):e17422
references.pmid: 21399691
references.citation: Nature. 2008 Apr 24;452(7190):1002-6
references.pmid: 18408712
references.citation: J Chem Ecol. 2010 Dec;36(12):1293-305
references.pmid: 20981477
references.citation: Cell. 2008 Jun 27;133(7):1255-1265
references.pmid: 18585358
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16538-43
references.pmid: 17060610
references.citation: Biochem Biophys Res Commun. 2005 Oct 7;335(4):1044-50
references.pmid: 16111659
references.citation: Chem Senses. 2004 Feb;29(2):117-25
references.pmid: 14977808
references.citation: Chem Biol. 2004 Aug;11(8):1029-31
references.pmid: 15324801
references.citation: Biosystems. 1997;40(1-2):203-10
references.pmid: 8971213
references.citation: Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5386-91
references.pmid: 15784736
references.citation: Front Cell Neurosci. 2011 Jun 16;5:5
references.pmid: 21720521
references.citation: Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8520-4
references.pmid: 1717980
references.citation: Chem Senses. 2011 Jul;36(6):497-8
references.pmid: 21441366
references.citation: Pest Manag Sci. 2002 Aug;58(8):839-44
references.pmid: 12192910