title: Impact of arachidonic acid enrichment of live rotifer prey on bacterial communities in rotifer and larval fish cultures.
abstract: Rotifers (Brachionus plicatilis), commonly used at first feeding in commercial fish hatcheries, carry a large bacteria load. Because they are relatively poor in essential fatty acids, it is common practice to enrich them with fatty acids, including arachidonic acid (AA). This study aims to determine whether prey enrichment with AA may act as a prebiotic and modify the microbial community composition either in AA-enriched rotifer cultures or in larval-rearing water using winter flounder (Pseudopleuronectes americanus) as a larval fish model. AA enrichment modified the bacterial community composition in both the rotifer culture tanks and the larval-rearing tanks. We observed an increase in the number of cultivable bacteria on TCBS (thiosulfate-citrate-bile salts-sucrose) agar, used as a proxy for the abundance of Vibrio sp. The results suggest that AA may also play an indirect role in larval health.
authors.affiliation: Phytopathology Institute, Working group in aquaculture, Kiel University, Olshausenstraße 40-60, 24118 Kiel, Germany.
authors.forename: Laurent H
authors.initials: LH
authors.lastname: Seychelles
authors.forename: Kim
authors.initials: K
authors.lastname: Doiron
authors.forename: Céline
authors.initials: C
authors.lastname: Audet
authors.forename: Réjean
authors.initials: R
authors.lastname: Tremblay
authors.forename: Fabrice
authors.initials: F
authors.lastname: Pernet
authors.forename: Karine
authors.initials: K
authors.lastname: Lemarchand
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D005227:Fatty Acids / Q000032:analysis; D005432:Flounder / Q000254:growth & development* / Q000382:microbiology; D007814:Larva / Q000382:microbiology; D012403:Rotifera / Q000737:chemistry / Q000187:drug effects* / Q000382:microbiology; D012623:Seawater / Q000382:microbiology
chemical_list: D005227:Fatty Acids; D016718:Arachidonic Acid