title: 1,8-Naphthalimide-Cu(ІІ) ensemble based turn-on fluorescent probe for the detection of thiols in organic aqueous media.
abstract: A 1,8-naphthalimide-Cu(II) ensemble was rationally designed and synthesized as a new turn-on fluorescent probe utilizing the 'chemosensing ensemble' method for detections of thiols (Cys, Hcy and GSH) with high selectivity over other α-amino acids at pH 7.4 in organic aqueous media (EtOH/HEPES, v/v=9:1). The recognition mechanism was attributed to the remove Cu(II) from the 1,8-naphthalimide-Cu(II) ensemble by thiols and the release of flurescence of ligand 1. Remarkable fluorescence enhancements were therefore observed in the sensing process of thiols by the 1,8-naphthalimide-Cu(II) ensemble. Furthermore, the 1,8-naphthalimide-Cu(II) ensemble was successfully applied to the fluorescence imaging of thiols in CHO cells with high sensitivity and selectivity.
authors.affiliation: Key Laboratory of Molecular Engineering and Photo-functional Materials, College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, PR China.
authors.forename: Yong Gang
authors.initials: YG
authors.lastname: Shi
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Yao
authors.forename: Yu Lian
authors.initials: YL
authors.lastname: Duan
authors.forename: Qi Li
authors.initials: QL
authors.lastname: Mi
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Chen
authors.forename: Quan Qing
authors.initials: QQ
authors.lastname: Xu
authors.forename: Gao Zhang
authors.initials: GZ
authors.lastname: Gou
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jun Feng
authors.initials: JF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016466:CHO Cells; D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D053644:Naphthalimides / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D013438:Sulfhydryl Compounds; D003300:Copper