title: α-Synuclein in CSF of patients with severe traumatic brain injury.
abstract: OBJECTIVE
  The study aims to examine α-synuclein in the CSF of patients with severe traumatic brain injury (TBI) and its relationship with clinical characteristics and long-term outcomes.
  
  
  METHODS
  This prospective case-control study enrolled patients with severe TBI (Glasgow Coma Score ≤ 8) who underwent ventriculostomy. CSF samples were taken from each TBI patient at admission and daily for up to 8 days after injury and successively assessed by ELISA. Control CSF was collected for analysis from subjects receiving lumbar puncture for other medical reasons. We used trajectory analysis to identify distinct temporal profiles of CSF α-synuclein that were compared with clinical outcomes.
  
  
  RESULTS
  CSF α-synuclein was elevated in TBI patients after injury as compared to controls (p = 0.0008). Overall, patients who died had higher concentrations (area under the curve) over 8 days of observation compared to those who survived at 6 months postinjury (p = 0.002). Two distinct temporal α-synuclein profiles were recognized over time. Subjects who died had consistently elevated α-synuclein levels compared to those who survived with α-synuclein levels near controls. High-risk trajectory was a strong and accurate predictor of death with 100% specificity and a very high sensitivity (83%).
  
  
  CONCLUSIONS
  Taken together, these data support the hypothesis that in severe TBI patients, substantial increase of CSF α-synuclein may indicate widespread neurodegeneration and reflect secondary neuropathologic events occurring after injury. The determination of CSF α-synuclein may be a valuable prognostic marker, adding to the clinical assessment and creating opportunities for medical intervention.
authors.affiliation: Clinical Department, Banyan Biomarkers, Inc., Alachua, FL, USA. stm_mondello@hotmail.com
authors.forename: Stefania
authors.initials: S
authors.lastname: Mondello
authors.forename: Andras
authors.initials: A
authors.lastname: Buki
authors.forename: Domenico
authors.initials: D
authors.lastname: Italiano
authors.forename: Andreas
authors.initials: A
authors.lastname: Jeromin
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000134:cerebrospinal fluid; D001930:Brain Injuries / Q000134:cerebrospinal fluid* / Q000401:mortality*; D016022:Case-Control Studies; D005260:Female; D015600:Glasgow Coma Scale; D006801:Humans; D008297:Male; D008875:Middle Aged; D009410:Nerve Degeneration / Q000134:cerebrospinal fluid / Q000401:mortality; D010865:Pilot Projects; D011379:Prognosis; D011446:Prospective Studies; D012307:Risk Factors; D016019:Survival Analysis; D055815:Young Adult; D051844:alpha-Synuclein / Q000134:cerebrospinal fluid*
chemical_list: D015415:Biomarkers; C497604:SNCA protein, human; D051844:alpha-Synuclein