title: Silencing of ADIPOQ efficiently suppresses preadipocyte differentiation in porcine.
abstract: OBJECTIVE
  Our study aims to characterize the functions of the ADIPOQ gene in the process of fat deposition of pigs, thereby providing a basis for the use of this gene as a molecular marker for pork quality.
  
  
  METHODS
  We used healthy Junmu1 piglets less than 7 days of age to establish an in vitro culture system for porcine preadipocytes. Chemically synthesized short hairpin RNAs (shRNA) were transfected into porcine preadipocytes to silence the expression of the ADIPOQ gene. We monitored preadipocyte differentiation and determined the levels of the adipocyte differentiation transcription factors lipoprotein lipase (LPL), peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte fatty acid binding protein (AP2) mRNAs to investigate the effects of ADIPOQ on the differentiation of porcine preadipocytes.
  
  
  RESULTS
  After transfection, the mRNA and protein levels of the ADIPOQ gene were significantly decreased (P < 0.01), the number of lipid droplets in the adipocytes was significantly reduced, the OD values reflecting the fat content were significantly decreased (P < 0.01), and the levels of LPL, PPARγ and AP2 were significantly reduced (P < 0.01).
  
  
  CONCLUSIONS
  These results suggest that interference with ADIPOQ gene expression can inhibit the differentiation of porcine preadipocytes.
authors.affiliation: College of Animal Sciences, Jilin University, Changchun 130062, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Fujuan
authors.initials: F
authors.lastname: Li
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lisheng
authors.initials: L
authors.lastname: Dai
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Huiyu
authors.initials: H
authors.lastname: Liu
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhang
authors.forename: Chengzhen
authors.initials: C
authors.lastname: Chen
authors.forename: Jiabao
authors.initials: J
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D052242:Adiponectin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D008460:Meat / Q000032:analysis; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D013552:Swine
chemical_list: D052242:Adiponectin; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D008071:Lipoprotein Lipase