title: Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography.
abstract: OBJECTIVE
  To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography.
  
  
  METHODS
  The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes - central graft thickness) was calculated and tracked over time.
  
  
  RESULTS
  Five DSAEK grafts were measured. The average central graft thickness was 188.7 ± 44.4 μm (range, 146-255 µm) immediately after microkeratome cuts. The average central graft thickness was 147.5 ± 33.0 μm (range, 116-190 µm) after 4 hours in preservation medium (P < 0.001). The average hourly change in central graft thickness was -30.5 μm (P = 0.0051), -8.6 μm (P = 0.055), -2.0 μm (P = 0.42), and 0.0 μm (P = 0.93) at 1, 2, 3, and 4 hours, respectively, after microkeratome cuts. The average hourly change in graft shape was insignificant.
  
  
  CONCLUSIONS
  DSAEK grafts become thinner after microkeratome cut and stabilize at approximately 2 hours. Therefore, DSAEK graft thickness should be measured at 1.5 to 3 hours after microkeratome cut.
authors.affiliation: Center for Ophthalmic Optics & Lasers, Casey Eye Institute and Department of Ophthalmology, Oregon Health & Science University, Portland, OR, USA.
authors.forename: Maolong
authors.initials: M
authors.lastname: Tang
authors.forename: Christopher
authors.initials: C
authors.lastname: Stoeger
authors.forename: Joshua
authors.initials: J
authors.lastname: Galloway
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Holiman
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Bald
authors.forename: David
authors.initials: D
authors.lastname: Huang
mesh_terms: D003315:Cornea / Q000473:pathology*; D015925:Cryopreservation; D057111:Descemet Stripping Endothelial Keratoplasty; D004108:Dilatation, Pathologic; D005583:Fourier Analysis; D006801:Humans; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D013997:Time Factors; D041623:Tomography, Optical Coherence
chemical_list: D019188:Organ Preservation Solutions
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