title: Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples.
abstract: Most Escherichia coli resistant to quinolones are not haemolytic. The objective of this study was to determine the phylogroup, clonal relationship, mechanism of quinolone resistance and virulence factors in 70 haemolytic E. coli resistant to nalidixic acid. Sixty-six isolates contained the hlyA gene, belonged to phylogroup B2, and 61 of them presented low-level resistance to fluoroquinolones. Four isolates presented high-level resistance to fluoroquinolones, contained the clyA gene and were included in phylogroup D. One single isolate (phylogroup D, with low level resistance to fluoroquinolones) contained both cytotoxins.
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IFIMAV, Santander, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Márquez-López
authors.forename: Belén
authors.initials: B
authors.lastname: Ruiz Del Castillo
authors.forename: María Eliecer
authors.initials: ME
authors.lastname: Cano
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodríguez-Mirones
authors.forename: Jesús
authors.initials: J
authors.lastname: Oteo
authors.forename: David
authors.initials: D
authors.lastname: Sáez
authors.forename: Luis
authors.initials: L
authors.lastname: Martínez-Martínez
keywords: Escherichia coli; Haemolysis; Quinolone resistance; clyA; hlyA
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