title: Effects of Fam83h overexpression on enamel and dentine formation.
abstract: OBJECTIVE
  The aim of this study was to determine if FAM83H over-expression causes dentine or enamel malformations.
  
  
  METHODS
  The full-length mouse Fam83h cDNA was inserted into the pCAGIG vector between a β-actin promoter and β-globin enhancer for ubiquitous expression in transgenic mice. Recombinant mouse FAM83H was expressed and used to generate polyclonal antibodies. Western blots showed enhanced expression of the Fam83h transgene. The effects of transgene expression on tooth development were assessed by microhardness measurements of enamel and dentine. Total thickness of incisor enamel at the level of the alveolar crest was measured and decussating rod patterns were visualized by scanning electron microscopy (SEM).
  
  
  RESULTS
  Three transgenic mouse lines were selected based upon their transgene expression levels. There was no statistically significant difference in the Vickers microhardness values of enamel or dentine between the transgenic lines or between the transgenic lines and wild type mice. No statistically significant differences in enamel thickness were observed between the transgenic lines and the wild type mice. SEM analysis revealed no apparent differences in the enamel crystal and rod morphologies.
  
  
  CONCLUSIONS
  Our findings demonstrate that over-expression of FAM83H in mice does not produce a phenotype in dentine or enamel.
authors.affiliation: Department of Pediatric Dentistry & Dental Research Institute School of Dentistry, Seoul National University, 275-1 Yongon-dong, Chongno-gu, Seoul, Republic of Korea. nillilia01@empas.com
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kweon
authors.forename: Kyung-Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Ko
authors.forename: Jan C-C
authors.initials: JC
authors.lastname: Hu
authors.forename: James P
authors.initials: JP
authors.lastname: Simmer
authors.forename: Jung-Wook
authors.initials: JW
authors.lastname: Kim
mesh_terms: D000566:Amelogenesis / Q000235:genetics*; D000567:Amelogenesis Imperfecta / Q000235:genetics*; D000704:Analysis of Variance; D000818:Animals; D003743:Dental Enamel / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D003804:Dentin / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D003810:Dentinogenesis / Q000235:genetics*; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D008855:Microscopy, Electron, Scanning; D011506:Proteins / Q000235:genetics*
chemical_list: C587940:FAM83h protein, mouse; D011506:Proteins
keywords: Amelogenesis imperfecta; Dentinogenesis; Family with sequence similarity 83, member h; Tooth
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