title: Isolation of phytase-producing bacteria from Himalayan soils and their effect on growth and phosphorus uptake of Indian mustard (Brassica juncea).
abstract: Phytase-producing bacteria (PPB) is being investigated as plant growth promoting rhizobacteria (PGPR) to improve the phosphorus (P) nutrition and growth of plants grown in soil with high phytate content. Phytate is dominant organic P forms in many soils and must be hydrolyzed to be available for plants. Indian mustard (Brassica juncea) is a plant with economic importance in agriculture and phytoremediation, therefore biotechnological tools to improve growth and environmental stress tolerance are needed. In this study, we isolated and characterized PPB from Himalayan soils and evaluated their effect on growth and P uptake by B. juncea under greenhouse conditions. Sixty five PPB were isolated and based on phytate hydrolysis, three efficient PPB were chosen and identified as Acromobacter sp. PB-01, Tetrathiobacter sp. PB-03 and Bacillus sp. PB-13. Selected PPB showed ability to grow at wide range of pH, temperature and salt concentrations as well as to harbour diverse PGPR activities, such as: solubilization of insoluble Ca-phosphate (193-642 μg ml(-1)), production of phytohormone indole acetic acid (5-39 μg ml(-1)) and siderophore. Tetrathiobacter sp. PB-03 and Bacillus sp. PB-13 showed 50 and 70 % inhibition of phytopathogen Rhizoctonia solani, respectively. Greenhouse potting assay also showed that the bacterization of B. juncea seeds with Tetrathiobacter sp. PB-03 and Bacillus sp. PB-13 significantly increased the biomass and P content in 30 days old seedlings. This study reveals the potential of PPB as PGPR to improve the growth of B. juncea.
authors.affiliation: Department of Biochemistry, G. B. Pant University of Agriculture and Technology, Pantnagar, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.forename: Prashant
authors.initials: P
authors.lastname: Singh
authors.forename: Milko A
authors.initials: MA
authors.lastname: Jorquera
authors.forename: Punesh
authors.initials: P
authors.lastname: Sangwan
authors.forename: Piyush
authors.initials: P
authors.lastname: Kumar
authors.forename: A K
authors.initials: AK
authors.lastname: Verma
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Agrawal
mesh_terms: D010832:6-Phytase / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D007194:India; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000254:growth & development* / Q000378:metabolism* / Q000382:microbiology; D010758:Phosphorus / Q000378:metabolism*; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D010758:Phosphorus; D010832:6-Phytase
references.citation: Microbiology (Reading). 1998 Jun;144 ( Pt 6):1565-1573
references.pmid: 9639927
references.citation: Trends Genet. 1992 Oct;8(10):332-3
references.pmid: 1475843
references.citation: Appl Environ Microbiol. 1998 Jun;64(6):2079-85
references.pmid: 9603817
references.citation: Appl Biochem Biotechnol. 2010 Mar;160(5):1267-76
references.pmid: 19333564
references.citation: FEMS Microbiol Lett. 1999 Jan 1;170(1):265-70
references.pmid: 9919677
references.citation: Appl Microbiol Biotechnol. 2004 Jan;63(4):362-72
references.pmid: 14586576
references.citation: Microbiol Res. 2003;158(3):243-8
references.pmid: 14521234
references.citation: J AOAC Int. 1994 May-Jun;77(3):760-4
references.pmid: 8012231
references.citation: Res Microbiol. 2008 May;159(4):244-50
references.pmid: 18490146
references.citation: Environ Microbiol. 2005 Mar;7(3):396-404
references.pmid: 15683400
references.citation: Microb Ecol. 2012 Nov;64(4):1008-17
references.pmid: 22639075
references.citation: Biotechnol Lett. 2003 Nov;25(21):1787-94
references.pmid: 14677699
references.citation: World J Microbiol Biotechnol. 2012 Mar;28(3):891-9
references.pmid: 22805809
references.citation: Indian J Microbiol. 2008 Sep;48(3):342-7
references.pmid: 23100732
references.citation: Microbiology (Reading). 2002 Jul;148(Pt 7):2097-2109
references.pmid: 12101298
references.citation: World J Microbiol Biotechnol. 2007 Dec;23(12):1653-60
references.pmid: 27517819
references.citation: Anal Biochem. 1987 Jan;160(1):47-56
references.pmid: 2952030
references.citation: Folia Microbiol (Praha). 2008;53(2):110-4
references.pmid: 18500629
references.citation: FEMS Microbiol Ecol. 2011 Jan;75(1):163-72
references.pmid: 21073489
references.citation: Can J Microbiol. 1997 Jun;43(6):509-16
references.pmid: 9226870
references.citation: Appl Environ Microbiol. 2002 Aug;68(8):3795-801
references.pmid: 12147474
references.citation: Philos Trans R Soc Lond B Biol Sci. 2002 Apr 29;357(1420):449-69
references.pmid: 12028785