title: Stochastic models of transcription: from single molecules to single cells.
abstract: Genes in prokaryotic and eukaryotic cells are typically regulated by complex promoters containing multiple binding sites for a variety of transcription factors leading to a specific functional dependence between regulatory inputs and transcriptional outputs. With increasing regularity, the transcriptional outputs from different promoters are being measured in quantitative detail in single-cell experiments thus providing the impetus for the development of quantitative models of transcription. We describe recent progress in developing models of transcriptional regulation that incorporate, to different degrees, the complexity of multi-state promoter dynamics, and its effect on the transcriptional outputs of single cells. The goal of these models is to predict the statistical properties of transcriptional outputs and characterize their variability in time and across a population of cells, as a function of the input concentrations of transcription factors. The interplay between mathematical models of different regulatory mechanisms and quantitative biophysical experiments holds the promise of elucidating the molecular-scale mechanisms of transcriptional regulation in cells, from bacteria to higher eukaryotes.
authors.affiliation: Department of Physics, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Sanchez
authors.forename: Sandeep
authors.initials: S
authors.lastname: Choubey
authors.forename: Jane
authors.initials: J
authors.lastname: Kondev
mesh_terms: D001419:Bacteria; D001665:Binding Sites; D056890:Eukaryota; D005786:Gene Expression Regulation; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D059010:Single-Cell Analysis; D013269:Stochastic Processes; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D014157:Transcription Factors
keywords: Chemical master equation; Single cell experiments; Stochastic models; Transcription; Transcriptional regulation