title: The ATP-binding cassette transporter ABCB19 regulates postembryonic organ separation in Arabidopsis.
abstract: The phytohormone auxin plays a critical role in plant development, including embryogenesis, organogenesis, tropism, apical dominance and in cell growth, division, and expansion. In these processes, the concentration gradient of auxin, which is established by polar auxin transport mediated by PIN-FORMED (PIN) proteins and several ATP-binding cassette/multi-drug resistance/P-glycoprotein (ABCB/MDR/PGP) transporters, is a crucial signal. Here, we characterized the function of ABCB19 in the control of Arabidopsis organ boundary development. We identified a new abcb19 allele, abcb19-5, which showed stem-cauline leaf and stem-pedicel fusion defects. By virtue of the DII-VENUS marker, the auxin level was found to be increased at the organ boundary region in the inflorescence apex. The expression of CUP-SHAPED COTYLEDON2 (CUC2) was decreased, while no obvious change in the expression of CUC3 was observed, in abcb19. In addition, the fusion defects were greatly enhanced in cuc3 abcb19-5, which was reminiscent of cuc2 cuc3. We also found that some other organ boundary genes, such as LOF1/2 were down-regulated in abcb19. Together, these results reveal a new aspect of auxin transporter ABCB19 function, which is largely dependent on the positive regulation of organ boundary genes CUC2 and LOFs at the postembryonic organ boundary.
authors.affiliation: Laboratory of Molecular Cell Biology, Hebei Normal University, Shijiazhuang, Hebei, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Huixian
authors.initials: H
authors.lastname: Mo
authors.forename: Litao
authors.initials: L
authors.lastname: Qian
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.forename: Sujuan
authors.initials: S
authors.lastname: Cui
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Ligeng
authors.initials: L
authors.lastname: Ma
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000483:Alleles; D017360:Arabidopsis / Q000187:drug effects / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D063245:Plant Development / Q000187:drug effects / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C532604:ABCB19 protein, Arabidopsis; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; C107294:CUC2 protein, Arabidopsis; C478975:CUC3 protein, Arabidopsis; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D020033:Protein Isoforms; D014157:Transcription Factors
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