title: Distinct set of kinases induced after retrieval of spatial memory discriminate memory modulation processes in the mouse hippocampus.
abstract: Protein phosphorylation and dephosphorylation events play a key role in memory formation and various protein kinases and phosphatases have been firmly associated with memory performance. Here, we determined expression changes of protein kinases and phosphatases following retrieval of spatial memory in CD1 mice in a Morris Water Maze task, using antibody microarrays and confirmatory Western blot. Comparing changes following single and consecutive retrieval, we identified stably and differentially expressed kinases, some of which have never been implicated before in memory functions. On the basis of these findings we define a small signaling network associated with spatial memory retrieval. Moreover, we describe differential regulation and correlation of expression levels with behavioral performance of polo-like kinase 1. Together with its recently observed genetic association to autism-spectrum disorders our data suggest a role of this kinase in balancing preservation and flexibility of learned behavior.
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Ajinkya
authors.initials: A
authors.lastname: Sase
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Patil
authors.forename: Berta
authors.initials: B
authors.lastname: Sunyer
authors.forename: Harald
authors.initials: H
authors.lastname: Höger
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Smalla
authors.forename: Oliver
authors.initials: O
authors.lastname: Stork
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D004192:Discrimination, Psychological / Q000502:physiology*; D005108:Extinction, Psychological; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D018782:Maze Learning; D011939:Mental Recall / Q000502:physiology*; D051379:Mice; D010766:Phosphorylation / Q000502:physiology; D040081:Protein Array Analysis; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013028:Space Perception / Q000502:physiology*; D013997:Time Factors; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1
keywords: CD1 mouse; Morris water maze; memory; protein kinases; protein phosphatases; spatial memory