title: Downregulation of miR-145 expression in oral squamous cell carcinomas and its clinical significance.
abstract: BACKGROUND
  MicroRNAs have been reported to play roles as oncogenes or tumor suppressor genes in human cancers. However, the expression levels of miR-145 in oral squamous cell carcinoma (OSCC) are unclear. The purpose of this study was to investigate the status of miR-145 expression in OSCC and determine its clinical significance.
  
  
  METHODS
  We examined miR-145 levels in 62 OSCC tissue samples and cell lines by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The relationship between miR-145 expression and clinicopathologic factors of OSCC patients was analyzed.
  
  
  RESULTS
  The proportion of miR-145 low expression was 82.26% (51/62) among the 62 OSCC patients, and expression levels of miR-145 in OSCC tissue samples and cell lines were significantly lower than in non-tumor controls. miR-145 expression levels were not significantly associated with age (p = 0.607), sex (p = 0.213), location (p = 0.952), histology (p = 0.603), pT stage (p = 0.305), pTNM stage (p = 0.471), and lymphatic metastasis (p = 1.000).
  
  
  CONCLUSIONS
  miR-145 may be involved in the early tumorigenesis of oral squamous cells, and might be a potential biomarker in the early diagnosis of OSCC.
authors.affiliation: Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an, Shaanxi, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Wenhao
authors.initials: W
authors.lastname: Ren
authors.forename: Su'e
authors.initials: S
authors.lastname: Chang
authors.forename: Bo
authors.initials: B
authors.lastname: Guo
authors.forename: Shuo
authors.initials: S
authors.lastname: Huang
authors.forename: Menghe
authors.initials: M
authors.lastname: Li
authors.forename: Youmin
authors.initials: Y
authors.lastname: Guo
authors.forename: Zongfang
authors.initials: Z
authors.lastname: Li
authors.forename: Tusheng
authors.initials: T
authors.lastname: Song
authors.forename: Keqian
authors.initials: K
authors.lastname: Zhi
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs