title: Quantification of intracellular mitochondrial displacements in response to nanomechanical forces.
abstract: Mechanical stress affects various aspects of cell behavior, including cell growth, morphology, differentiation, and genetic expression. Here, we describe a method to quantify the intracellular mechanical response to an extracellular mechanical perturbation, specifically the displacement of mitochondria. A combined fluorescent-atomic force microscope (AFM) was used to simultaneously produce well-defined nanomechanical stimulation to a living cell while optically recording the real-time displacement of fluorescently labeled mitochondria. A single-particle tracking (SPT) approach was then applied in order to quantify the two-dimensional displacement of mitochondria in response to local forces.
authors.affiliation: Biomedical Research Institute (BMRI), National Institute of Advanced Industrial Science and Technology (AIST), Kyoto University, Kyoto, Japan.
authors.forename: Yaron R
authors.initials: YR
authors.lastname: Silberberg
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Pelling
mesh_terms: D000818:Animals; D051379:Mice; D018625:Microscopy, Atomic Force; D008928:Mitochondria / Q000378:metabolism*; D041681:NIH 3T3 Cells; D036103:Nanotechnology