title: Oral probiotic microcapsule formulation ameliorates non-alcoholic fatty liver disease in Bio F1B Golden Syrian hamsters.
abstract: The beneficial effect of a microencapsulated feruloyl esterase producing Lactobacillus fermentum ATCC 11976 formulation for use in non-alcoholic fatty liver disease (NAFLD) was investigated. For which Bio F1B Golden Syrian hamsters were fed a methionine deficient/choline devoid diet to induce non-alcoholic fatty liver disease. Results, for the first time, show significant clinical benefits in experimental animals. Examination of lipids show that concentrations of hepatic free cholesterol, esterified cholesterol, triglycerides and phospholipids were significantly lowered in treated animals. In addition, serum total cholesterol, triglycerides, uric acid and insulin resistance were found to decrease in treated animals. Liver histology evaluations showed reduced fat deposits. Western blot analysis shows significant differences in expression levels of key liver enzymes in treated animals. In conclusion, these findings suggest the excellent potential of using an oral probiotic formulation to ameliorate NAFLD.
authors.affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Québec, Canada.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Bhathena
authors.forename: Christopher
authors.initials: C
authors.lastname: Martoni
authors.forename: Arun
authors.initials: A
authors.lastname: Kulamarva
authors.forename: Catherine
authors.initials: C
authors.lastname: Tomaro-Duchesneau
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Malhotra
authors.forename: Arghya
authors.initials: A
authors.lastname: Paul
authors.forename: Aleksandra Malgorzata
authors.initials: AM
authors.lastname: Urbanska
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002214:Capsules; D006224:Cricetinae; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D052197:Limosilactobacillus fermentum; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008647:Mesocricetus; D065626:Non-alcoholic Fatty Liver Disease; D019936:Probiotics / Q000494:pharmacology*
chemical_list: D002214:Capsules
references.citation: J Agric Food Chem. 2003 Aug 27;51(18):5534-9
references.pmid: 12926910
references.citation: BMJ. 2011 Jul 18;343:d3897
references.pmid: 21768191
references.citation: Phytother Res. 2004 Apr;18(4):310-4
references.pmid: 15162367
references.citation: Diabetes. 2001 Aug;50(8):1844-50
references.pmid: 11473047
references.citation: J Lipid Res. 2000 Dec;41(12):1991-2001
references.pmid: 11108732
references.citation: J Nutr. 2002 Jul;132(7):1962-8
references.pmid: 12097677
references.citation: Expert Opin Biol Ther. 2012 Sep;12(9):1207-20
references.pmid: 22690730
references.citation: J Pharm (Cairo). 2013;2013:103527
references.pmid: 26555963
references.citation: J Microencapsul. 2005 Sep;22(6):603-19
references.pmid: 16401577
references.citation: Expert Opin Biol Ther. 2013 May;13(5):631-42
references.pmid: 23350815
references.citation: Biofactors. 2004;21(1-4):315-9
references.pmid: 15630218
references.citation: J Biomed Biotechnol. 2011;2011:981214
references.pmid: 21772792
references.citation: J Hepatol. 2006 Jun;44(6):1167-74
references.pmid: 16618517
references.citation: J Clin Gastroenterol. 2006 Mar;40 Suppl 1:S39-43
references.pmid: 16540766
references.citation: Am J Physiol Endocrinol Metab. 2003 Oct;285(4):E906-16
references.pmid: 12959938
references.citation: Biotechnol Appl Biochem. 2008 May;50(Pt 1):1-9
references.pmid: 17640174
references.citation: Nihon Rinsho. 2008 Apr;66(4):766-70
references.pmid: 18409529
references.citation: Liver Int. 2006 Jun;26(5):566-71
references.pmid: 16762001
references.citation: Br J Nutr. 2008 May;99(5):1013-7
references.pmid: 17977471
references.citation: Eksp Klin Gastroenterol. 2010;(7):25-33
references.pmid: 21033080
references.citation: J Nutr. 2009 May;139(5):905-11
references.pmid: 19321579
references.citation: Cochrane Database Syst Rev. 2007 Jan 24;(1):CD005165
references.pmid: 17253543
references.citation: FASEB J. 2005 Oct;19(12):1746-8
references.pmid: 16055499
references.citation: Postgrad Med J. 2004 Sep;80(947):516-26
references.pmid: 15356352
references.citation: Lett Appl Microbiol. 2012 Jan;54(1):18-25
references.pmid: 22017704
references.citation: Free Radic Biol Med. 2001 Aug 1;31(3):304-14
references.pmid: 11461767
references.citation: Pharmaceuticals (Basel). 2012 Feb 16;5(2):236-48
references.pmid: 24288090
references.citation: Kidney Int. 2003 Jul;64(1):192-8
references.pmid: 12787409
references.citation: J Nutr. 2003 Feb;133(2):468-75
references.pmid: 12566485
references.citation: Obes Rev. 2011 Apr;12(4):272-81
references.pmid: 20804522
references.citation: Hepatology. 2003 Feb;37(2):343-50
references.pmid: 12540784
references.citation: J Agric Food Chem. 2003 Sep 24;51(20):6050-5
references.pmid: 13129315
references.citation: Nutr Rev. 2007 Aug;65(8 Pt 1):376-84
references.pmid: 17867371
references.citation: Nat Med. 1996 Aug;2(8):883-7
references.pmid: 8705857
references.citation: Rev Endocr Metab Disord. 2004 Dec;5(4):311-8
references.pmid: 15486463
references.citation: Hepatology. 2007 Jul;46(1):158-65
references.pmid: 17596883
references.citation: Appl Microbiol Biotechnol. 2007 Jul;75(5):1023-9
references.pmid: 17483939
references.citation: J Clin Gastroenterol. 2005 Jul;39(6):540-3
references.pmid: 15942443
references.citation: Curr Opin Lipidol. 2010 Feb;21(1):76-83
references.pmid: 19915460
references.citation: Diabetes Metab Syndr Obes. 2011;4:195-203
references.pmid: 21760736
references.citation: J Nutr Biochem. 2011 Aug;22(8):699-711
references.pmid: 21292470