title: Identification and antifungal susceptibility of fungi isolated from dermatomycoses.
abstract: BACKGROUND
  Dermatomycoses are superficial fungal infections of the skin, hair and nails that affect more than 20-25% of the people worldwide. These infections can be caused by yeasts, dermatophytes and non-dermatophyte filamentous fungi (NDFF) and are considered a public health problem. Despite this, few studies have investigated the prevalence and antifungal susceptibility of causative agents of dermatomycoses in the developing world.
  
  
  OBJECTIVE
  The aims of this study were to identify and determine the antifungal susceptibility profile of yeast and filamentous fungi isolated from dermatomycoses in Uberaba, Minas Gerais, Brazil.
  
  
  METHODS
  Specimens were obtained from patients with clinically diagnosed and laboratory confirmed dermatomycosis between July 2009 and July 2011. Fungal identification was based on classical methods and antifungal susceptibility testing was performed by broth microdilution method.
  
  
  RESULTS
  Of the 216 fungal isolates, 116 (53.8%) were yeasts, 70 (32.4%) dermatophytes and 30 (13.8%) NDFF. Onychomycosis was the most common clinical condition. Candida parapsilosis (24.1%) and Trichophyton rubrum (17.1%) were the fungi most frequently isolated. Voriconazole, ketoconazole and itraconazole were the most potent antifungal agents against yeast, whereas terbinafine, voriconazole and itraconazole had a high in vitro activity against dermatophytes. Overall, the antifungal agents had little or no activity against NDFF and the highest minimum inhibitory concentrations were those against Fusarium spp.
  
  
  CONCLUSIONS
  Yeasts, particularly C. parapsilosis, play an important role as causative agents of dermatomycosis in our region. Our results suggest that the antifungal susceptibility testing coupled with proper identification of the fungi may be useful to assist clinicians in determining the appropriate therapy for dermatomycoses.
authors.affiliation: Laboratory of Microbiology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
authors.forename: L B
authors.initials: LB
authors.lastname: Silva
authors.forename: D B C
authors.initials: DB
authors.lastname: de Oliveira
authors.forename: B V
authors.initials: BV
authors.lastname: da Silva
authors.forename: R A
authors.initials: RA
authors.lastname: de Souza
authors.forename: P R
authors.initials: PR
authors.lastname: da Silva
authors.forename: K
authors.initials: K
authors.lastname: Ferreira-Paim
authors.forename: L E
authors.initials: LE
authors.lastname: Andrade-Silva
authors.forename: M L
authors.initials: ML
authors.lastname: Silva-Vergara
authors.forename: A A
authors.initials: AA
authors.lastname: Andrade
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003881:Dermatomycoses / Q000188:drug therapy* / Q000382:microbiology; D005658:Fungi / Q000145:classification / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents