title: Different distributions of Epstein-Barr virus early and late gene transcripts within viral replication compartments.
abstract: Productive replication of the Epstein-Barr virus (EBV) occurs in discrete sites in nuclei, called replication compartments, where viral genome DNA synthesis and transcription take place. The replication compartments include subnuclear domains, designated BMRF1 cores, which are highly enriched in the BMRF1 protein. During viral lytic replication, newly synthesized viral DNA genomes are organized around and then stored inside BMRF1 cores. Here, we examined spatial distribution of viral early and late gene mRNAs within replication compartments using confocal laser scanning microscopy and three-dimensional surface reconstruction imaging. EBV early mRNAs were mainly located outside the BMRF1 cores, while viral late mRNAs were identified inside, corresponding well with the fact that late gene transcription is dependent on viral DNA replication. From these results, we speculate that sites for viral early and late gene transcription are separated with reference to BMRF1 cores.
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, Nagoya, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Sugimoto
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Sato
authors.forename: Teru
authors.initials: T
authors.lastname: Kanda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Murata
authors.forename: Yohei
authors.initials: Y
authors.lastname: Narita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kawashima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tsurumi
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure* / Q000821:virology*; D004261:DNA Replication; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D021621:Imaging, Three-Dimensional; D017404:In Situ Hybridization, Fluorescence; D018613:Microscopy, Confocal; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; C083204:Epstein-Barr virus early antigen diffuse component; D012333:RNA, Messenger; D014764:Viral Proteins
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