title: Adhesion strength-based, label-free isolation of human pluripotent stem cells.
abstract: We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over ∼10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.
authors.affiliation: Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
authors.forename: Ankur
authors.initials: A
authors.lastname: Singh
authors.forename: Shalu
authors.initials: S
authors.lastname: Suri
authors.forename: Ted
authors.initials: T
authors.lastname: Lee
authors.forename: Jamie M
authors.initials: JM
authors.lastname: Chilton
authors.forename: Marissa T
authors.initials: MT
authors.lastname: Cooke
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Fu
authors.forename: Steven L
authors.initials: SL
authors.lastname: Stice
authors.forename: Hang
authors.initials: H
authors.lastname: Lu
authors.forename: Todd C
authors.initials: TC
authors.lastname: McDevitt
authors.forename: Andrés J
authors.initials: AJ
authors.lastname: García
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D002469:Cell Separation; D006801:Humans; D007621:Karyotyping; D039904:Pluripotent Stem Cells / Q000166:cytology*
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