title: The effects of α-defensin 1 on electrical field stimulation-induced contraction of rat bladders.
abstract: This study was designed to investigate the effects of α-defensin 1 on electrical field stimulation (EFS)-induced contractions in isolated rat bladder detrusor muscles. We evaluated the effects of α-defensin 1 (50 pM∼5 nM) on EFS-induced contractions in the detrusor smooth muscles from 35 rats (2-30 Hz). Bladder strips were pretreated with α-defensin 1 and then changes of contractions by adenosine 5'-triphosphate (ATP) were observed. Moreover, after pretreatment with α-defensin 1 for 10 min, changes in concentration-response curves to hydrogen peroxide (H2O2) were investigated. Alpha-defensin 1 has increased EFS-induced contractions, significantly, and the contractile response to ATP (1,2,5,10mM) was also increased significantly when strips were pretreated with α-defensin 1. In addition, alpha-defensin 1 increased H2O2-induced contractions. The present study demonstrates that α-defensin 1 increases EFS-induced contractions of rat detrusor muscles via purinergic contraction coupled with the Rho kinase pathway.
authors.affiliation: Department of Urology, Seoul Medical Center, Seoul, Republic of Korea.
authors.forename: Shin Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyung Do
authors.initials: KD
authors.lastname: Kim
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Moo Yeol
authors.initials: MY
authors.lastname: Lee
authors.forename: Hae Jong
authors.initials: HJ
authors.lastname: Kim
authors.forename: June Hyun
authors.initials: JH
authors.lastname: Han
authors.forename: Soon Chul
authors.initials: SC
authors.lastname: Myung
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D023084:alpha-Defensins / Q000494:pharmacology*
chemical_list: D023084:alpha-Defensins; C406413:human neutrophil peptide 1; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide
keywords: Bladder overactivity; EFS (Electrical field stimulation); Purinergic contraction; Rat bladder muscle; α-defensin 1 (HNP1)