title: Photoluminescent gold nanoclusters as sensing probes for uropathogenic Escherichia coli.
abstract: Glycan-bound nanoprobes have been demonstrated as suitable sensing probes for bacteria containing glycan binding sites. In this study, we demonstrated a facile approach for generating glycan-bound gold nanoclusters (AuNCs). The generated AuNCs were used as sensing probes for corresponding target bacteria. Mannose-capped AuNCs (AuNCs@Mann) were generated and used as the model sensors for target bacteria. A one-step synthesis approach was employed to generate AuNCs@Mann. In this approach, an aqueous solution of tetrachloroauric acid and mannoside that functionized with a thiol group (Mann-SH) was stirred at room temperature for 48 h. The mannoside functions as reducing and capping agent. The size of the generated AuNCs@Mann is 1.95±0.27 nm, whereas the AuNCs with red photoluminescence have a maximum emission wavelength of ~630 nm (λexcitation = 375 nm). The synthesis of the AuNCs@Mann was accelerated by microwave heating, which enabled the synthesis of the AuNCs@Mann to complete within 1 h. The generated AuNCs@Mann are capable of selectively binding to the urinary tract infection isolate Escherichia coli J96 containing the mannose binding protein FimH expressed on the type 1 pili. On the basis of the naked eye observation, the limit of detection of the sensing approach is as low as ~2×10(6) cells/mL.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Po-Han
authors.initials: PH
authors.lastname: Chan
authors.forename: Bhaswati
authors.initials: B
authors.lastname: Ghosh
authors.forename: Hong-Zheng
authors.initials: HZ
authors.lastname: Lai
authors.forename: Hwei-Ling
authors.initials: HL
authors.lastname: Peng
authors.forename: Kwok Kong Tony
authors.initials: KK
authors.lastname: Mong
authors.forename: Yu-Chie
authors.initials: YC
authors.lastname: Chen
mesh_terms: D018830:Adhesins, Escherichia coli / Q000096:biosynthesis / Q000737:chemistry*; D015373:Bacterial Typing Techniques / Q000379:methods*; D036781:Fimbriae Proteins / Q000096:biosynthesis / Q000737:chemistry*; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008358:Mannose / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D056689:Uropathogenic Escherichia coli / Q000145:classification* / Q000378:metabolism
chemical_list: D018830:Adhesins, Escherichia coli; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; D006046:Gold; D008358:Mannose
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