title: Characterization of gastric and neuronal histaminergic populations using a transgenic mouse model.
abstract: Histamine is a potent biogenic amine that mediates numerous physiological processes throughout the body, including digestion, sleep, and immunity. It is synthesized by gastric enterochromaffin-like cells, a specific set of hypothalamic neurons, as well as a subset of white blood cells, including mast cells. Much remains to be learned about these varied histamine-producing cell populations. Here, we report the validation of a transgenic mouse line in which Cre recombinase expression has been targeted to cells expressing histidine decarboxylase (HDC), which catalyzes the rate-limiting step in the synthesis of histamine. This was achieved by crossing the HDC-Cre mouse line with Rosa26-tdTomato reporter mice, thus resulting in the expression of the fluorescent Tomato (Tmt) signal in cells containing Cre recombinase activity. As expected, the Tmt signal co-localized with HDC-immunoreactivity within the gastric mucosa and gastric submucosa and also within the tuberomamillary nucleus of the brain. HDC expression within Tmt-positive gastric cells was further confirmed by quantitative PCR analysis of mRNA isolated from highly purified populations of Tmt-positive cells obtained by fluorescent activated cell sorting (FACS). HDC expression within these FACS-separated cells was found to coincide with other markers of both ECL cells and mast cells. Gastrin expression was co-localized with HDC expression in a subset of histaminergic gastric mucosal cells. We suggest that these transgenic mice will facilitate future studies aimed at investigating the function of histamine-producing cells.
authors.affiliation: The Department of Internal Medicine (Division of Hypothalamic Research and Division of Endocrinology & Metabolism) and the Department of Psychiatry, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Angela K
authors.initials: AK
authors.lastname: Walker
authors.forename: Won-Mee
authors.initials: WM
authors.lastname: Park
authors.forename: Jen-Chieh
authors.initials: JC
authors.lastname: Chuang
authors.forename: Mario
authors.initials: M
authors.lastname: Perello
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sakata
authors.forename: Sherri
authors.initials: S
authors.lastname: Osborne-Lawrence
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Zigman
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction
chemical_list: D006632:Histamine; C045073:Cre recombinase; D019426:Integrases; D006640:Histidine Decarboxylase
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