title: Multiple roles of RNase Y in Streptococcus pyogenes mRNA processing and degradation.
abstract: Control over mRNA stability is an essential part of gene regulation that involves both endo- and exoribonucleases. RNase Y is a recently identified endoribonuclease in Gram-positive bacteria, and an RNase Y ortholog has been identified in Streptococcus pyogenes (group A streptococcus [GAS]). In this study, we used microarray and Northern blot analyses to determine the S. pyogenes mRNA half-life of the transcriptome and to understand the role of RNase Y in global mRNA degradation and processing. We demonstrated that S. pyogenes has an unusually high mRNA turnover rate, with median and mean half-lives of 0.88 min and 1.26 min, respectively. A mutation of the RNase Y-encoding gene (rny) led to a 2-fold increase in overall mRNA stability. RNase Y was also found to play a significant role in the mRNA processing of virulence-associated genes as well as in the rapid degradation of rnpB read-through transcripts. From these results, we conclude that RNase Y is a pleiotropic regulator required for mRNA stability, mRNA processing, and removal of read-through transcripts in S. pyogenes.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA. Zhiyun-Chen@ouhsc.edu
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Chen
authors.forename: Andreas
authors.initials: A
authors.lastname: Itzek
authors.forename: Horst
authors.initials: H
authors.lastname: Malke
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Ferretti
authors.forename: Jens
authors.initials: J
authors.lastname: Kreth
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006207:Half-Life; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D020871:RNA Stability; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D012329:RNA, Bacterial; D012333:RNA, Messenger; D004722:Endoribonucleases
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