title: Restoration of miR-1228* expression suppresses epithelial-mesenchymal transition in gastric cancer.
abstract: Dysregulated miRNAs play critical roles during carcinogenesis and cancer progression. In the present study, the function of miR-1228* in regulating cancer progression was investigated in gastric cancer. Decreased expression of miR-1228* was observed in human gastric cancer tissues comparing to normal tissues. Subsequently, the role of miR-1228* was evaluated in vivo using the tumor xenograft model. In this model, miR-1228* overexpression suppressed xenograft tumor formation. Furthermore, we demonstrated miR-1228* negatively regulated NF-κB activity in SGC-7901 gastric cancer cells and found that CK2A2 was a target of miR-1228*. Upregulation of miR-1228* decreased the expression of mesenchymal markers and increased the epithelial marker E-cadherin, suggesting its potential role in suppressing epithelial-mesenchymal transition. Collectively, these findings provide the first evidence that miR-1228* plays an important role in regulating gastric cancer growth and suggest that selective restoration of miR-1228* might be beneficial for gastric cancer therapy.
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University College of Medicine, Hangzhou, China.
authors.forename: Litao
authors.initials: L
authors.lastname: Jia
authors.forename: Jia
authors.initials: J
authors.lastname: Wu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Jiamin
authors.initials: J
authors.lastname: Chen
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhong
authors.forename: Song
authors.initials: S
authors.lastname: Xu
authors.forename: Chuangao
authors.initials: C
authors.lastname: Xie
authors.forename: Jianting
authors.initials: J
authors.lastname: Cai
mesh_terms: D000818:Animals; D015820:Cadherins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis*; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D013274:Stomach Neoplasms; D014183:Transplantation, Heterologous; D015854:Up-Regulation
chemical_list: D015820:Cadherins; C576906:MIRN1228 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm
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