title: T-type ca(2+) channel blockers increase smooth muscle progenitor cells and endothelial progenitor cells in bone marrow stromal cells in culture by suppression of cell death.
abstract: OBJECTIVE
  To examine the expression patterns and roles of voltage-dependent Ca2+ channels in bone marrow stromal cells (BMSCs).
  
  
  METHODS
  Ca(2+) currents of BMSCs were measured by the whole-cell patch clamp method. The number and percentage of deaths of BMSCs cultured for 14 days with or without Ca(2+) channel blockers were evaluated using a MTT assay and an LDH assay, respectively.
  
  
  RESULTS
  T-type Ca(2+) channel current was recorded in 0, 2, 10, and 4% of BMSCs on days 3, 10, 17, and 24 in culture, respectively. L-type Ca(2+) channel current was first recorded on day 24 in 6% of BMSCs. Addition of the T-type Ca(2+) channel blocker mibefradil but not the L-type Ca(2+) channel blocker nifedipine significantly increased the cell count. Immunocytochemical analysis revealed increases in the counts of smooth muscle progenitor cells (SMPCs) and endothelial progenitor cells (EPCs). Mibefradil but not nifedipine significantly decreased the rate of cell death.
  
  
  CONCLUSIONS
  T-type Ca(2+) channel blockers increased the numbers of SMPCs and EPCs in cultured BMSCs, partly through suppression of cell death. Thus, T-type Ca(2+) channel blockers may have the potential to provide an increased number of both BMSC-derived SMCs and ECs of potential use in cell and gene therapy.
authors.affiliation: Department of Physiology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Hashimoto
authors.forename: Youichi
authors.initials: Y
authors.lastname: Katoh
authors.forename: Seigo
authors.initials: S
authors.lastname: Itoh
authors.forename: Takafumi
authors.initials: T
authors.lastname: Iesaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Daida
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakazato
authors.forename: Takao
authors.initials: T
authors.lastname: Okada
keywords: T-type Ca2+ channel blocker; bone marrow stromal cells; endothelial progenitor cells; smooth muscle progenitor cells
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