title: Pannexin-1 influences peritoneal cavity cell population but is not involved in NLRP3 inflammasome activation.
abstract: Pannexin-1 (Panx1) forms nonselective large channel in cell plasma membrane and has been shown to be associated with NLRP3 inflammasome activation, ATP release and phagocytes recruitment. In the current study, by manipulation of Panx1 expression in human myeloid cells and application of Panx1 deficient mice, we failed to find a correlation between Panx1 and NLRP3 inflammasome activation, although an interaction between these two proteins was evident. However, in thioglycollate induced peritonitis, Panx1 deficient mice showed much more phagocytes infiltration. Further analyses showed that mice deficient for Panx1 exhibited enlarged F4/80(low)Gr1(-)Ly6C(-)cell population in the peritonea. Our study thus reveals an important role for Panx1 in regulation of peritoneal cell population and peritonitis development.
authors.affiliation: Unit of Innate Immunity, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai 200025, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Xing
authors.forename: Liming
authors.initials: L
authors.lastname: Mao
authors.forename: Yi
authors.initials: Y
authors.lastname: Luo
authors.forename: Lishan
authors.initials: L
authors.lastname: Kang
authors.forename: Guangxun
authors.initials: G
authors.lastname: Meng
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D017630:Connexins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology*; D010538:Peritonitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D013864:Thioglycolates / Q000633:toxicity
chemical_list: D002352:Carrier Proteins; D017630:Connexins; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D009419:Nerve Tissue Proteins; C492764:PANX1 protein, human; C503631:Panx1 protein, mouse; D034741:RNA, Small Interfering; D013864:Thioglycolates
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