title: Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses.
abstract: This study aimed to investigate anti-Toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in Brazil. We collected brain and blood samples from 398 horses of various ages, from six Brazilian states. Serum samples were evaluated by indirect fluorescent antibody test (IFAT cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. Among the 398 horses, positivity for T. gondii was identified in 46 (11.6%) by IFAT and in 14 (3.5%) by mouse bioassay. In 12 of those 14 bioassays, mice were positive only by IFAT (cut-off titre ≥ 1:16), T. gondii being isolated in the remaining two. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rDNA to differentiate among T. gondii, Neospora caninum, and Sarcocystis neurona, we found that two of the 14 brains were positive for T. gondii only. For genotyping of the two isolates and the PCR-positive brain, we performed PCR-RFLP based on 13 markers, and SAG2 all samples were Toxoplasma gondii type I. Collectively, IFAT of horse sera and mouse bioassay identified positivity in 60 (15%) of the samples. Our results show that some horses sent to slaughter in Brazil have been exposed to T. gondii.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Evers
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.forename: Italmar Teodorico
authors.initials: IT
authors.lastname: Navarro
authors.forename: Dauton Luiz
authors.initials: DL
authors.lastname: Zulpo
authors.forename: Beatriz de Souza Lima
authors.initials: Bde S
authors.lastname: Nino
authors.forename: Maria Paula de Carvalho
authors.initials: MP
authors.lastname: Ewald
authors.forename: Sthefany
authors.initials: S
authors.lastname: Pagliari
authors.forename: Jonatas Campos de
authors.initials: JC
authors.lastname: Almeida
authors.forename: Roberta Lemos
authors.initials: RL
authors.lastname: Freire
mesh_terms: D000003:Abattoirs; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001938:Brazil; D006734:Horse Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology*; D006736:Horses; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan