title: Susceptibility of Brazilian isolates of Trypanosoma evansi to suramin sodium: test in experimentally infected mice.
abstract: This study aimed to evaluate the susceptibility of Brazilian isolates of Trypanosoma evansi to suramin sodium. For this purpose, three isolates of T. evansi (LPV-2005, LPV-2009 and LPV-2010) and seventy mice were used, with the animals divided in 10 groups (A, B, C, D, E, F, G, H, I and J) with seven animals each group. Mice of groups A, B, and C were infected with LPV-2005; Groups D, E and F with LPV-2009 and the groups G, H and I with LPV-2010. The group J was composed by healthy mice or uninfected. The parasitemia was monitored daily through blood smear, and the treatment of all groups was performed three days post-infection (PI), when all mice showed increased parasitemia. Groups A, D and G represented the positives controls, while groups B, E and H received a single dose of suramin sodium at 10 mgkg(-1) intramuscularly. Groups C, F and I were treated with three doses of suramin sodium at 10 mgkg(-1), respecting an interval of 24 h between each dose. Negative blood smears from all animals were obtained 24 h after treatment (AT), status maintained until the end of the experiment (50 days PI). The specific PCR for T. evansi was carried out from blood, showing negative results AT. Therefore, this study showed that a single dose of suramin sodium at 10 mgkg(-1) has the same efficacy of three doses, as recommended by the therapeutic literature. Furthermore, we observed that Brazilian isolates did not show resistance to the drug.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, Brazil. lucianabfaccio@hotmail.com
authors.forename: Luciana
authors.initials: L
authors.lastname: Faccio
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Lucas T
authors.initials: LT
authors.lastname: Gressler
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Tonin
authors.forename: Cícera R
authors.initials: CR
authors.lastname: Lazzarotto
authors.forename: Luiz Claudio
authors.initials: LC
authors.lastname: Miletti
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D001938:Brazil; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D018512:Parasitemia / Q000188:drug therapy / Q000469:parasitology; D013498:Suramin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D014345:Trypanosoma / Q000187:drug effects*; D014352:Trypanosomiasis / Q000188:drug therapy* / Q000469:parasitology
chemical_list: D014344:Trypanocidal Agents; D013498:Suramin