title: Impact of inulin and okara on Lactobacillus acidophilus La-5 and Bifidobacterium animalis Bb-12 viability in a fermented soy product and probiotic survival under in vitro simulated gastrointestinal conditions.
abstract: The effect of inulin and/or okara flour on Lactobacillus acidophilus La-5 and Bifidobacterium animalis Bb-12 viability in a fermented soy product (FSP) and on probiotic survival under in vitro simulated gastrointestinal conditions were investigated throughout 28 days of storage at 4 °C. Employing a 2(2) design, four FSP trials were produced from soymilk fermented with ABT-4 culture (La-5, Bb-12, and Streptococcus thermophilus): FSP (control); FSP-I (with inulin, 3 g/100 mL of soymilk); FSP-O (with okara, 5 g/100 mL); FSP-IO (with inulin + okara, ratio 3:5 g/100 mL). Probiotic viabilities ranged from 8 to 9 log cfu/g during the 28 days of storage, and inulin and/or okara flour did not affect the viability of La-5 and Bb-12. Bb-12 resistance to the artificial gastrointestinal juices was higher than for La-5, since the Bb-12 and La-5 populations decreased approximately 0.6 log cfu/g and 3.8 log cfu/g, respectively, throughout storage period. Even though the protective effect of inulin and/or okara flour on probiotic microorganisms was not significant, when compared to a fresh culture, the FSP matrix improved Bb-12 survival on day 1 of storage and may be considered a good vehicle for Bb-12 and could play an important role in probiotic protection against gastrointestinal juices.
authors.affiliation: Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, 05508-000 São Paulo, SP, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Bedani
authors.forename: Elizeu Antonio
authors.initials: EA
authors.lastname: Rossi
authors.forename: Susana Marta
authors.initials: SM
authors.lastname: Isay Saad
mesh_terms: D001644:Bifidobacterium / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D007444:Inulin / Q000378:metabolism*; D007779:Lactobacillus acidophilus / Q000254:growth & development / Q000378:metabolism*; D050296:Microbial Viability; D008954:Models, Biological; D010940:Plant Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D019936:Probiotics / Q000032:analysis*; D045730:Soy Foods; D041942:Soy Milk / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D011134:Polysaccharides; C412882:okara, Glycine max; D007444:Inulin