title: Structure analysis and antimutagenic activity of a novel salt-soluble polysaccharide from Auricularia polytricha.
abstract: BACKGROUND
  Auricularia polytricha is known to be a highly nutritious foodstuff. We report here the purification, structure characterization and antimutagenic activity in vivo of a 0.9% NaCl solution-soluble polysaccharide (SSP) from the mycelia of A. polytricha.
  
  
  RESULTS
  Analysis by high-performance liquid chromatography coupled to a TSK-G5000PWXL column and gel filtration chromatography on Sephacryl S-400 HR indicated that SSP is homogeneous with an average molecular weight of about 9.30 × 10(5) Da. The structure of SSP was revealed by chemical methods, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Results indicated that SSP is a glucan consisting of a1,3-β-glucan, 1,6-α-glucan, 1,4-α-glucan and 1,3-α-glucan backbone with a single 1,6-α-d-glucopyranosyl side-branching unit on every nine residues, on average, along the main chain. Atomic force microscopy indicates the presence of macromolecular species in morphology and shows a clear association of prolate particle. Meanwhile, SSP was found to significantly preventing micronuclei in polychromatic erythrocytes and reticulocytes of mice (P < 0.01).
  
  
  CONCLUSIONS
  These results indicate that polysaccharide SSP from A. polytricha exhibits antimutagenic activity against the in vivo DNA-damaging effect of the indirectly acting alkylating agent cyclophosphamide.
authors.affiliation: Institute of Food and Biological Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310035, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Mengting
authors.initials: M
authors.lastname: Xin
authors.forename: Qinyao
authors.initials: Q
authors.lastname: Luo
authors.forename: Jialu
authors.initials: J
authors.lastname: Gu
authors.forename: Mengtian
authors.initials: M
authors.lastname: Zhao
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Xu
authors.forename: Xuanqi
authors.initials: X
authors.lastname: Lu
authors.forename: Guanglei
authors.initials: G
authors.lastname: Song
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents; D001487:Basidiomycota / Q000737:chemistry*; D002236:Carbohydrate Conformation; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D005936:Glucans / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D025282:Mycelium / Q000737:chemistry; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D012965:Sodium Chloride; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D016587:Antimutagenic Agents; D062610:Fungal Polysaccharides; D005936:Glucans; D009005:Monosaccharides; D010504:Periodic Acid; D012965:Sodium Chloride; C009288:metaperiodate
keywords: Auricularia polytricha; antimutagenic activity; polysaccharide; structure