title: SOX7 is down-regulated in lung cancer.
abstract: BACKGROUND
  SOX7 is a transcription factor belonging to the SOX family. Its role in lung cancer is unknown.
  
  
  METHODS
  In this study, whole genomic copy number analysis was performed on a series of non-small cell lung cancer (NSCLC) cell lines and samples from individuals with epidermal growth factor receptor (EGFR) mutations using a SNP-Chip platform. SOX7 was measured in NSCLC samples and cell lines, and forced expressed in one of these lines.
  
  
  RESULTS
  A notable surprise was that the numerous copy number (CN) changes observed in samples of Asian, non-smoking EGFR mutant NSCLC were nearly the same as those CN alterations seen in a large collection of NSCLC from The Cancer Genome Atlas which is presumably composed of predominantly Caucasians who often smoked. However, four regions had CN changes fairly unique to the Asian EGFR mutant group. We also examined CN changes in NSCLC lines. The SOX7 gene was homozygously deleted in one (HCC2935) of 10 NSCLC cell lines and heterozygously deleted in two other NSCLC lines. Expression of SOX7 was significantly downregulated in NSCLC cell lines (8/10, 80%) and a large collection of NSCLC samples compared to matched normal lung (57/62, 92%, p= 0.0006). Forced-expression of SOX7 in NSCLC cell lines markedly reduced their cell growth and enhanced their apoptosis.
  
  
  CONCLUSIONS
  These data suggest that SOX7 is a novel tumor suppressor gene silenced in the majority of NSCLC samples.
authors.affiliation: Genomic Oncology Programme, Cancer Science Institute of Singapore, Singapore, Singapore.
authors.forename: Takahide
authors.initials: T
authors.lastname: Hayano
authors.forename: Manoj
authors.initials: M
authors.lastname: Garg
authors.forename: Dong
authors.initials: D
authors.lastname: Yin
authors.forename: Makoto
authors.initials: M
authors.lastname: Sudo
authors.forename: Norihiko
authors.initials: N
authors.lastname: Kawamata
authors.forename: Shuo
authors.initials: S
authors.lastname: Shi
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chien
authors.forename: Ling-Wen
authors.initials: LW
authors.lastname: Ding
authors.forename: Geraldine
authors.initials: G
authors.lastname: Leong
authors.forename: Seiichi
authors.initials: S
authors.lastname: Mori
authors.forename: Dong
authors.initials: D
authors.lastname: Xie
authors.forename: Patrick
authors.initials: P
authors.lastname: Tan
authors.forename: H Phillip
authors.initials: HP
authors.lastname: Koeffler
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D056915:DNA Copy Number Variations; D019175:DNA Methylation; D015536:Down-Regulation; D066246:ErbB Receptors / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055106:Genome-Wide Association Study; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D055760:SOXF Transcription Factors / Q000235:genetics*; D012907:Smoking
chemical_list: C484456:SOX7 protein, human; D055760:SOXF Transcription Factors; D066246:ErbB Receptors
references.citation: Blood. 2009 Nov 26;114(23):4813-22
references.pmid: 19801444
references.citation: Cell Stem Cell. 2008 Aug 7;3(2):182-95
references.pmid: 18682240
references.citation: Am J Hum Genet. 2007 Jul;81(1):114-26
references.pmid: 17564968
references.citation: Blood. 2008 Mar 1;111(5):2657-66
references.pmid: 18094332
references.citation: Stem Cells. 2009 Jun;27(6):1231-43
references.pmid: 19489079
references.citation: Clin Cancer Res. 2011 Jun 1;17(11):3542-50
references.pmid: 21521776
references.citation: Chin J Cancer. 2011 May;30(5):287-92
references.pmid: 21527061
references.citation: Mol Cancer Res. 2008 Sep;6(9):1421-30
references.pmid: 18819930
references.citation: Bioinformatics. 2002 Nov;18(11):1427-31
references.pmid: 12424112
references.citation: Differentiation. 2009 Mar;77(3):248-55
references.pmid: 19272523
references.citation: Cancer Res. 2005 Jul 15;65(14):6071-9
references.pmid: 16024607
references.citation: Cancer Lett. 2009 May 8;277(1):29-37
references.pmid: 19108950
references.citation: J Neurosci Res. 2009 Nov 15;87(15):3277-87
references.pmid: 19437544
references.citation: Dev Biol. 2000 Nov 15;227(2):239-55
references.pmid: 11071752
references.citation: Int J Biochem Cell Biol. 2010 Mar;42(3):445-8
references.pmid: 19733255