title: Combined cell culture-biosensing platform using vertically aligned patterned peptide nanofibers for cellular studies.
abstract: This Article presents the development of a combined cell culture-biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture-biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsted Plads 345B. 2800 Kgs. Lyngby, Denmark.
authors.forename: Mehmet B
authors.initials: MB
authors.lastname: Taskin
authors.forename: Luigi
authors.initials: L
authors.lastname: Sasso
authors.forename: Maria
authors.initials: M
authors.lastname: Dimaki
authors.forename: Winnie E
authors.initials: WE
authors.lastname: Svendsen
authors.forename: Jaime
authors.initials: J
authors.lastname: Castillo-León
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D049109:Cell Proliferation; D002477:Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D008839:Microelectrodes; D057139:Nanofibers / Q000737:chemistry*; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry*; D051381:Rats
chemical_list: D010455:Peptides; D004298:Dopamine