title: Enzymatic synthesis of novel phloretin glucosides.
abstract: A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.
authors.affiliation: Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, Tangjeong-myeon, Asansi, Chungnam, Republic of Korea.
authors.forename: Ramesh Prasad
authors.initials: RP
authors.lastname: Pandey
authors.forename: Tai Feng
authors.initials: TF
authors.lastname: Li
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Kim
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Yong Il
authors.initials: YI
authors.lastname: Park
authors.forename: Joong Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005818:Genetic Engineering; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010693:Phloretin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D014539:Uridine Diphosphate Sugars; C583941:YjiC protein, E coli; D016695:Glycosyltransferases; D010693:Phloretin
references.citation: Biochem J. 1997 Sep 15;326 ( Pt 3):929-39
references.pmid: 9334165
references.citation: Annu Rev Nutr. 2001;21:381-406
references.pmid: 11375442
references.citation: J Mol Biol. 2003 Apr 25;328(2):307-17
references.pmid: 12691742
references.citation: J Chromatogr A. 1998 Oct 9;823(1-2):331-7
references.pmid: 9818410
references.citation: J Biol Chem. 2003 Dec 5;278(49):49154-63
references.pmid: 12954647
references.citation: Biochem Biophys Res Commun. 2002 Jul 5;295(1):9-13
references.pmid: 12083758
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8
references.pmid: 18838391
references.citation: Anticancer Res. 2001 Nov-Dec;21(6A):3949-56
references.pmid: 11911276
references.citation: J Nutr. 2005 Feb;135(2):172-8
references.pmid: 15671209
references.citation: J Agric Food Chem. 2003 Oct 8;51(21):6347-53
references.pmid: 14518966
references.citation: Curr Opin Biotechnol. 2005 Dec;16(6):622-30
references.pmid: 16226456
references.citation: J Agric Food Chem. 2003 May 7;51(10):2896-9
references.pmid: 12720368
references.citation: J Am Coll Cardiol. 2000 Jun;35(7):1977-85
references.pmid: 10841251
references.citation: Appl Environ Microbiol. 2012 Nov;78(21):7680-6
references.pmid: 22923401
references.citation: Appl Microbiol Biotechnol. 2013 Mar;97(5):1889-901
references.pmid: 23053089
references.citation: Nat Rev Drug Discov. 2010 Jul;9(7):551-9
references.pmid: 20508640
references.citation: ACS Chem Biol. 2011 Jan 21;6(1):95-100
references.pmid: 20886903
references.citation: Trends Plant Sci. 2000 Sep;5(9):380-6
references.pmid: 10973093
references.citation: Infect Immun. 2011 Dec;79(12):4819-27
references.pmid: 21930760
references.citation: Nat Prod Rep. 1997 Apr;14(2):99-110
references.pmid: 9149408