title: Extraction optimization of carbohydrate compound from Huangqi using orthogonal design.
abstract: The effect of extraction time, extraction temperature and time on the yield of Huangqi carbohydrate compound (HQCC) was investigated using single factor and orthogonal experiment design. The influence by the parameters on the extraction yields of carbohydrate compound decreased in the order of: C (extraction number)>A (extraction time)>B (extraction temperature) according to the R values. Based on this analysis, and considering the carbohydrate compound extraction efficiency, the cost of energy and the feasibility of experiment, the optimum conditions of extraction were therefore determined as follows: extraction time 120min, extraction temperature 80°C, and extraction number 4. Oral administration of HQCC reduced lipid peroxidation level and enhanced antioxidant enzymes activities in gastric mucosa. In addition, HQCC reduced the serum IL-8 and TNF-α levels. In conclusion, these data reveal that HQCC promotes regeneration of damaged gastric mucosa, probably through its antioxidative mechanism.
authors.affiliation: Gastrointestinal Surgery of Taizhou Hospital of Zhejiang Province, Linhai, Zhejiang Province, PR China.
authors.forename: Shen-kang
authors.initials: SK
authors.lastname: Zhou
authors.forename: Tie-Nan
authors.initials: TN
authors.lastname: Bi
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Xu
authors.forename: Rui-Li
authors.initials: RL
authors.lastname: Zhang
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry; D027885:Astragalus propinquus; D002374:Catalase / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D007213:Indomethacin; D008315:Malondialdehyde / Q000097:blood; D008516:Medicine, Chinese Traditional; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D052616:Solid Phase Extraction; D013276:Stomach Ulcer / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D008315:Malondialdehyde; C027492:Huang Qi; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D007213:Indomethacin