title: Texcoconibacillus texcoconensis gen. nov., sp. nov., alkalophilic and halotolerant bacteria isolated from soil of the former lake Texcoco (Mexico).
abstract: A novel Gram-positive, rod-shaped, spore-forming bacterium, designated 13CC(T) was isolated from soil of the former lake Texcoco. The strain was aerobic, catalase-positive and oxidase-negative. It grew at salinities of 0-26% (w/v) NaCl with an optimum at 9-16% (w/v) NaCl. The cells contain peptidoglycan type A1γ, A1γ' with glycine instead of l-alanine and three variations of peptidoglycan type A4γ. The only quinone detected was MK-7. The major fatty acid was anteiso-C(15:0). The polar lipids fraction consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three different phospholipids. The DNA G+C content was 37.5 mol%. Maximum-likelihood phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 13CC(T) was closely related to members of the genus Bacillus and shared 92.35% similarity with Bacillus agaradhaerens, 92.28% with Bacillus neizhouensis and 92.21% with Bacillus locisalis. It is proposed based on the phenotypic, genotypic and phylogenetic analyses that the novel isolate should be classified as a representative of a new genus and novel species, for which the name Texcoconibacillus texcoconensis gen. nov., sp. nov. is proposed. The type strain of Texcoconibacillus texcoconensis is 13CC(T) ( =JCM 17654(T) =DSM 24696(T)).
authors.affiliation: Laboratory of Soil Ecology, Cinvestav, Mexico.
authors.forename: Erick
authors.initials: E
authors.lastname: Ruiz-Romero
authors.affiliation: Laboratory of Soil Ecology, Cinvestav, Mexico.
authors.forename: María de Los Angeles
authors.initials: MLA
authors.lastname: Coutiño-Coutiño
authors.affiliation: Laboratory of Soil Ecology, Cinvestav, Mexico.
authors.forename: César
authors.initials: C
authors.lastname: Valenzuela-Encinas
authors.affiliation: Laboratory of Soil Ecology, Cinvestav, Mexico.
authors.forename: María Patricia
authors.initials: MP
authors.lastname: López-Ramírez
authors.affiliation: Laboratory of Soil Ecology, Cinvestav, Mexico.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Marsch
authors.affiliation: Laboratory of Soil Ecology, Cinvestav, Mexico.
authors.forename: Luc
authors.initials: L
authors.lastname: Dendooven
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060106:Lakes / Q000382:microbiology*; D008800:Mexico; D008969:Molecular Sequence Data; D010457:Peptidoglycan; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7