title: Phase diagram and tie-line determination for the ternary mixture DOPC/eSM/cholesterol.
abstract: We propose a novel, to our knowledge, method for the determination of tie lines in a phase diagram of ternary lipid mixtures. The method was applied to a system consisting of dioleoylphosphatidylcholine (DOPC), egg sphingomyelin (eSM), and cholesterol (Chol). The approach is based on electrofusion of single- or two-component homogeneous giant vesicles in the fluid phase and analyses of the domain areas of the fused vesicle. The electrofusion approach enables us to create three-component vesicles with precisely controlled composition, in contrast to conventional methods for giant vesicle formation. The tie lines determined in the two-liquid-phase coexistence region are found to be not parallel, suggesting that the dominant mechanism of lipid phase separation in this region changes with the membrane composition. We provide a phase diagram of the DOPC/eSM/Chol mixture and predict the location of the critical point. Finally, we evaluate the Gibbs free energy of transfer of individual lipid components from one phase to the other.
authors.affiliation: Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Bezlyepkina
authors.forename: R S
authors.initials: RS
authors.lastname: Gracià
authors.forename: P
authors.initials: P
authors.lastname: Shchelokovskyy
authors.forename: R
authors.initials: R
authors.lastname: Lipowsky
authors.forename: R
authors.initials: R
authors.lastname: Dimova
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry*; D010063:Ovum / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D010713:Phosphatidylcholines; D013109:Sphingomyelins; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine
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