title: Disruption of the integrity and function of brain microvascular endothelial cells in culture by exposure to diesel engine exhaust particles.
abstract: Diesel exhaust particles (DEPs), a by-product of diesel engine exhaust (DEE), are known to produce pro-oxidative and pro-inflammatory effects, thereby leading to oxidative stress-induced damage. Given the key role of DEPs in inducing oxidative stress, we investigated the role of DEPs in disrupting the integrity and function of immortalized human brain microvascular endothelial cells (HBMVEC). To study this, HBMVEC cells were exposed to media containing three different concentrations of DEPs or plain media for 24h. Those exposed to DEPs showed significantly higher oxidative stress than the untreated group, as indicated by the glutathione (GSH) and malondialdehyde (MDA) levels, and the glutathione peroxidase and glutathione reductase activities. DEPs also induced oxidative stress-related disruption of the HBMVEC cells monolayer, as measured by trans-epithelial electrical resistance. Taken together, these data suggest that DEPs induce cell death and disrupt the function and integrity of HBMVEC cells, indicating a potential role of DEPs in neurotoxicities.
authors.affiliation: Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA.
authors.forename: Shakila
authors.initials: S
authors.lastname: Tobwala
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Youyou
authors.initials: Y
authors.lastname: Zheng
authors.forename: Hsiu-Jen
authors.initials: HJ
authors.lastname: Wang
authors.forename: William A
authors.initials: WA
authors.lastname: Banks
authors.forename: Nuran
authors.initials: N
authors.lastname: Ercal
mesh_terms: D001921:Brain / Q000098:blood supply / Q000187:drug effects*; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003911:Dextrans / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D017097:Electric Impedance; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D055806:Microvessels / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D003911:Dextrans; D001335:Vehicle Emissions; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione
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