title: The immune response to pneumococcal polysaccharides 14 and 23F among elderly individuals consists predominantly of switched memory B cells.
abstract: The phenotype of B cells that respond to vaccination with the purified pneumococcal polysaccharide (PPS) has been a topic of debate. We have recently identified the phenotype of cells from healthy young volunteers as CD27(+)IgM(+) B cells. However, the PPS-responding B-cell population has not yet been identified in high-risk populations, such as elderly individuals. Previous studies have shown that elderly individuals have a lower percentage of immunoglobulin M memory B cells than healthy young adults. In this study, we directly characterized the phenotype of PPS-specific B cells before and after vaccination with PPS vaccine (PPV) in elderly adults, using fluorescently labeled PPS14 and PPS23F. In contrast to our observations in healthy young volunteers, the PPS-responding B-cell population consisted primarily of switched memory (CD27(+)IgM(-)) B cells. In concurrence with these findings, postvaccination immunoglobulin M concentrations were not significantly increased in this population, and the opsonophagocytic response was decreased, compared with that in young adults. These findings identify a significant shift in the phenotype of the B-cell population in response to PPV among elderly individuals.
authors.affiliation: Department of Medicine, University of Toledo, Ohio 43614, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Leggat
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Thompson
authors.forename: Noor M
authors.initials: NM
authors.lastname: Khaskhely
authors.forename: Anita S
authors.initials: AS
authors.lastname: Iyer
authors.forename: M A Julie
authors.initials: MA
authors.lastname: Westerink
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D016667:Bacterial Capsules / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007111:Immunity, Cellular; D007156:Immunologic Memory / Q000276:immunology / Q000502:physiology; D018655:Lymphocyte Count; D008875:Middle Aged; D022242:Pneumococcal Vaccines / Q000276:immunology*
chemical_list: C414006:23-valent pneumococcal capsular polysaccharide vaccine; D000907:Antibodies, Bacterial; D022242:Pneumococcal Vaccines; C056696:pneumococcal polysaccharide, type 14
keywords: 23F; B cell; CD27; Pneumococcus; Streptococcus pneumoniae; elderly; flow cytometry; human; polysaccharide; vaccine
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