title: Chemiluminescence and ELISA-based serum assays for diagnosing and monitoring celiac disease in children: a comparative study.
abstract: BACKGROUND
  Anti-transglutaminase (tTG) or anti-deamidated gliadin peptides (DGP) serum determination is the first step in diagnosing celiac disease (CD). Our aims were to: compare the performance of novel chemiluminescent tool in the detection of tTG and DGP (Q-Flash®, Inova) with that of the established ELISA (Q-Lite®, Inova) methods; identify the more reliable index for making a sound diagnosis and monitoring therapy.
  
  
  METHODS
  Using Q-Flash® and Q-Lite®, IgA and IgG class tTG and DGP were measured in the sera of 155 CD pediatric patients and 166 healthy age-matched controls. Forty-two of the patients had a follow-up one year after starting gluten free diet (GFD).
  
  
  RESULTS
  Q-Flash® IgA tTG, the more accurate (intra-assay CV for low, intermediate and high values: 2.2%, 1.6%, and 1.1%; inter-assay CV: 2.8%, 4%, and 3%), sensitive (96.1%) and specific (97%) test for diagnosing CD, was the only variable to be significantly correlated with CD at binary logistic regression analysis (r=0.263, p<0.0001, Exp(B)=1.0506, 95% CI=1.0286-1.0731). Q-Flash® IgA tTG or DGP screen were more accurate than Q-Lite® IgA tTG in monitoring CD patients on GFD (p=0.003).
  
  
  CONCLUSIONS
  Q- Flash® IgA tTG measurement is an extremely precise, sensitive and specific index for not only diagnosing CD, but also monitoring therapy.
authors.affiliation: Department of Medicine-DIMED, University of Padova, Padova, Italy.
authors.forename: Ada
authors.initials: A
authors.lastname: Aita
authors.forename: Elisa
authors.initials: E
authors.lastname: Rossi
authors.forename: Daniela
authors.initials: D
authors.lastname: Basso
authors.forename: Graziella
authors.initials: G
authors.lastname: Guariso
authors.forename: Dania
authors.initials: D
authors.lastname: Bozzato
authors.forename: Michela
authors.initials: M
authors.lastname: Pelloso
authors.forename: Matilde
authors.initials: M
authors.lastname: Pescarin
authors.forename: Carlo-Federico
authors.initials: CF
authors.lastname: Zambon
authors.forename: Filippo
authors.initials: F
authors.lastname: Navaglia
authors.forename: Eliana
authors.initials: E
authors.lastname: Greco
authors.forename: Marco
authors.initials: M
authors.lastname: Gasparetto
authors.forename: Paola
authors.initials: P
authors.lastname: Fogar
authors.forename: Andrea
authors.initials: A
authors.lastname: Padoan
authors.forename: Stefania
authors.initials: S
authors.lastname: Moz
authors.forename: Mario
authors.initials: M
authors.lastname: Plebani
mesh_terms: D016022:Case-Control Studies; D002446:Celiac Disease / Q000097:blood* / Q000175:diagnosis / Q000178:diet therapy / Q000276:immunology; D002648:Child; D055050:Diet, Gluten-Free; D019468:Disease Management; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005260:Female; D005903:Gliadin / Q000097:blood / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D008163:Luminescent Measurements / Q000592:standards*; D008297:Male; D015588:Observer Variation; D015203:Reproducibility of Results; D011503:Transglutaminases / Q000097:blood / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin; D011503:Transglutaminases