title: Reversed phase High Performance Liquid Chromatography used for the physicochemical and thermodynamic characterization of piceatannol/β-cyclodextrin complex.
abstract: Piceatannol is one of resveratrol derivatives having health promoting potential. However, its low water-solubility and bioavailability could limit its use in both food and pharmaceutical fields. The aim of this work is the study of piceatannol complexation by β-cyclodextrin (β-CD) in aqueous media. The complex formed could improve the bioavailability, the solubility and the stability of piceatannol. The method used was based on RP-HPLC in which, β-CD was added to methanol/water mixtures mobile phases. The apparent formation constant of piceatannol/β-cyclodextrin complex was determined. Within the concentration interval studied (0-4 mM), whenever the concentration of β-CD increased, retention time of piceatannol decreased; indicating an enhancement of solubility due to the formation of the piceatannol/β-CD complex. The formation constants (KF) of the piceatannol/β-CD complex varied significantly with both the methanol concentration in the water-methanol mixture and the temperature. Moreover, in all the physicochemical conditions tested, the stoichiometry of piceatannol/β-CD complex was 1:1. Finally, the thermodynamic parameters were determined: ΔG°=-13.123 kJ mol(-1), ΔH°=-33. 265 kJ mol(-1) and ΔS°=-67.559 J mol(-1) K(-1).
authors.affiliation: Laboratory of Applied Chemistry, 8 Mai 1945 Guelma University, Guelma 24000, Algeria.
authors.forename: Hanane
authors.initials: H
authors.lastname: Messiad
authors.forename: Habiba
authors.initials: H
authors.lastname: Amira-Guebailia
authors.forename: Omar
authors.initials: O
authors.lastname: Houache
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry*; D013816:Thermodynamics; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D013267:Stilbenes; D047392:beta-Cyclodextrins; C041525:3,3',4,5'-tetrahydroxystilbene; C031215:betadex; D000077185:Resveratrol