title: Methods for culturing human embryonic stem cells on feeders.
abstract: Human embryonic stem cells can be cultured and maintained on fibroblast feeder cells of murine or human origin. Thorough protocols are provided for the growth and maintenance of human embryonic stem cells on either feeder cell type.
authors.affiliation: Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA.
authors.forename: Jasmeet
authors.initials: J
authors.lastname: Kaur
authors.forename: Mary Lynn
authors.initials: ML
authors.lastname: Tilkins
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D015925:Cryopreservation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D061252:Feeder Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D052816:Foreskin / Q000166:cytology; D006801:Humans; D008297:Male; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects; D046509:Tissue Culture Techniques; D014599:Uterus / Q000166:cytology
chemical_list: D016685:Mitomycin