title: Genetic structuring of nine Indian domestic goat breeds based on SNPs identified in IGF-1 gene.
abstract: The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); three medium sized breeds (Sirohi, Barbari, and Osmanabadi) and three small sized breeds (Black Bengal, Changthangi, and Gaddi) were screened for SNP identification and diversity analysis. The comparative gene sequence analysis of all the nine goat breeds studied revealed a total of 18 SNPs in IGF1 gene. All the nucleotide changes were found to be synonymous. The mean observed heterozygosity was found to be maximum (0.074) in Sirohi, Beetal, Osmanabadi, and Gaddi breeds of goat, whereas it is found to be minimum (0.019) in Black Bengal breed of goat. The rest of the breeds were intermediate in terms of heterozygosity. The same has been confirmed by allele frequency distribution across the studied loci. Barbari and Gaddi were found to be more differentiated (0.0123), Changthangi and Jamunapari were least differentiated (0.00110) based on Nei's genetic distance.
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, Haryana, India.
authors.forename: Anurodh
authors.initials: A
authors.lastname: Sharma
authors.forename: Gautam
authors.initials: G
authors.lastname: Dutt
authors.forename: S
authors.initials: S
authors.lastname: Jayakumar
authors.forename: Vinita
authors.initials: V
authors.lastname: Saroha
authors.forename: N K
authors.initials: NK
authors.lastname: Verma
authors.forename: S P
authors.initials: SP
authors.lastname: Dixit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D006041:Goats / Q000235:genetics*; D007194:India; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D007334:Insulin-Like Growth Factor I; D004247:DNA