title: Trypanosoma cruzi trans-sialidase initiates a program independent of the transcription factors RORγt and Ahr that leads to IL-17 production by activated B cells.
abstract: Here we identified B cells as a major source of rapid, innate-like production of interleukin 17 (IL-17) in vivo in response to infection with Trypanosoma cruzi. IL-17(+) B cells had a plasmablast phenotype, outnumbered cells of the TH17 subset of helper T cells and were required for an optimal response to this pathogen. With both mouse and human primary B cells, we found that exposure to parasite-derived trans-sialidase in vitro was sufficient to trigger modification of the cell-surface mucin CD45, which led to signaling dependent on the kinase Btk and production of IL-17A or IL-17F via a transcriptional program independent of the transcription factors RORγt and Ahr. Our combined data suggest that the generation of IL-17(+) B cells may be a previously unappreciated feature of innate immune responses required for pathogen control or IL-17-mediated autoimmunity.
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Bermejo
authors.forename: Shaun W
authors.initials: SW
authors.lastname: Jackson
authors.forename: Melisa
authors.initials: M
authors.lastname: Gorosito-Serran
authors.forename: Eva V
authors.initials: EV
authors.lastname: Acosta-Rodriguez
authors.forename: Maria C
authors.initials: MC
authors.lastname: Amezcua-Vesely
authors.forename: Blythe D
authors.initials: BD
authors.lastname: Sather
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Singh
authors.forename: Socheath
authors.initials: S
authors.lastname: Khim
authors.forename: Juan
authors.initials: J
authors.lastname: Mucci
authors.forename: Denny
authors.initials: D
authors.lastname: Liggitt
authors.forename: Oscar
authors.initials: O
authors.lastname: Campetella
authors.forename: Mohamed
authors.initials: M
authors.lastname: Oukka
authors.forename: Adriana
authors.initials: A
authors.lastname: Gruppi
authors.forename: David J
authors.initials: DJ
authors.lastname: Rawlings
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000469:parasitology; D049109:Cell Proliferation; D002478:Cells, Cultured; D014355:Chagas Disease / Q000235:genetics / Q000276:immunology*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism*; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000469:parasitology; D058504:Th17 Cells / Q000276:immunology / Q000469:parasitology; D015533:Transcriptional Activation / Q000276:immunology; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000276:immunology*
chemical_list: D006023:Glycoproteins; D020381:Interleukin-17; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D018336:Receptors, Aryl Hydrocarbon; C069715:trans-sialidase; D009439:Neuraminidase
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