title: A novel method for preparing complete antigens of gonyautoxin 2,3 and their feature of immunogenicity.
abstract: In this paper, a novel method was proposed to prepare artificial antigens of gonyaulax parlaytic shellfish toxin 2 and 3 (GTX2,3). An intermediate GTX2,3-aldehyde was first synthesized by activating the NH₂ group of the 2nd and 8th amino acid residues with three different aldehydes and two artificial complete antigens GTX2,3-aldehyde-bovine serum albumin (BSA) and GTX2,3-aldehyde- keyhole limpet hemocyanin (KLH) were then prepared by cross-linking the intermediate with BSA or KLH. The successful preparation of the two complete antigens was confirmed by UV spectral scanning, HPLC, production of antibodies with titer of 1.28 × 10⁴ from mice immunized with the two complete antigens, indirect ELISA and Western-blot. In conclusion, the synthesized complete antigens have strong immunogenicity, which provides a solid foundation for preparing GTX2,3 monoclonal antibody and rapid detection kit.
authors.affiliation: Center of Modern Biochemistry of Experimental Teaching Department of Guangdong Ocean University, Zhanjiang 524088, China. zhangr1168@163.com
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Caohua
authors.initials: C
authors.lastname: Zhang
authors.forename: Xihong
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Qin
authors.forename: Huajuan
authors.initials: H
authors.lastname: Lin
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000917:Antibody Formation / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology; D012758:Shellfish
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D012530:Saxitoxin; C043405:gonyautoxin II