title: Aerobic metabolism and oxidative stress tolerance in the Lactobacillus plantarum group.
abstract: Aerobic metabolism and response to oxidative stress and starvation were studied in 11 Lactobacillus plantarum, L. paraplantarum and L. pentosus strains in order to assess the impact of aerobic metabolism on the growth and on the stress response. The strains were grown in aerobiosis without supplementation (AE), with hemin (AEH) or with hemin and menaquinone (AEHM) supplementation and in anaerobiosis (AN) in a complex buffered substrate. Growth rate, biomass yield, glucose and O₂ consumption, production of lactic acid and H₂O₂, catalase activity, oxidative and starvation stress tolerance were evaluated. Aerobic growth increased biomass yield in late stationary phase. Further increase in yield was obtained with both hemin (H) and menaquinone (M) addition. With few exceptions, the increase in biomass correlated with the decrease of lactic acid which, however, decreased in anaerobic conditions as well in some strains. Addition of H or H + M increased growth rate for some strains but reduced the duration of the lag phase. H₂O₂ production was found only for aerobic growth with no supplementation due to catalase production when hemin was supplemented. To our knowledge this is the first study in which the advantages of aerobic growth with H or H + M in improving tolerance of oxidative stress and long-term survival is demonstrated on several strains of the L. plantarum group. The results may have significant technological consequences for both starter and probiotic production.
authors.affiliation: Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy. angela.guidone@unibas.it
authors.forename: A
authors.initials: A
authors.lastname: Guidone
authors.forename: R G
authors.initials: RG
authors.lastname: Ianniello
authors.forename: A
authors.initials: A
authors.lastname: Ricciardi
authors.forename: T
authors.initials: T
authors.lastname: Zotta
authors.forename: E
authors.initials: E
authors.lastname: Parente
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D018533:Biomass; D002374:Catalase / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006427:Hemin; D006861:Hydrogen Peroxide / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D015999:Multivariate Analysis; D018384:Oxidative Stress; D010101:Oxygen Consumption; D019936:Probiotics / Q000378:metabolism; D024482:Vitamin K 2
chemical_list: D003470:Culture Media; D024482:Vitamin K 2; D019344:Lactic Acid; D006427:Hemin; D006861:Hydrogen Peroxide; D002374:Catalase
references.citation: Aust J Biol Sci. 1983;36(5-6):487-96
references.pmid: 6426447
references.citation: Microb Cell Fact. 2009 May 29;8:28
references.pmid: 19480672
references.citation: Biotechnol Appl Biochem. 2008 May;50(Pt 1):25-33
references.pmid: 17824842
references.citation: Protein Sci. 1992 Dec;1(12):1699-709
references.pmid: 1304899
references.citation: Appl Environ Microbiol. 2006 Dec;72(12):7933-40
references.pmid: 17012588
references.citation: Appl Environ Microbiol. 2004 Jan;70(1):603-6
references.pmid: 14711694
references.citation: J Bacteriol. 2004 Oct;186(19):6661-6
references.pmid: 15375150
references.citation: Antonie Van Leeuwenhoek. 2002 Aug;82(1-4):263-9
references.pmid: 12369192
references.citation: J Bacteriol. 2007 Jul;189(14):5203-9
references.pmid: 17496098
references.citation: Mol Biotechnol. 2010 Jun;45(2):155-60
references.pmid: 20195800
references.citation: World J Microbiol Biotechnol. 2012 Mar;28(3):919-27
references.pmid: 22805812
references.citation: Antonie Van Leeuwenhoek. 1997 Feb;71(1-2):117-28
references.pmid: 9049023
references.citation: Int J Food Microbiol. 1994 Nov;23(3-4):277-94
references.pmid: 7873331
references.citation: Appl Environ Microbiol. 1992 Mar;58(3):832-9
references.pmid: 1575485
references.citation: Int J Food Microbiol. 1994 Dec;24(1-2):191-8
references.pmid: 7703013
references.citation: Antonie Van Leeuwenhoek. 1983 Sep;49(3):209-24
references.pmid: 6354079
references.citation: J Biol Chem. 1983 May 25;258(10):6015-9
references.pmid: 6853475
references.citation: Mol Microbiol. 2004 Sep;53(5):1331-42
references.pmid: 15387813
references.citation: J Bacteriol. 2004 Jun;186(12):3749-59
references.pmid: 15175288
references.citation: J Bacteriol. 1983 Aug;155(2):742-6
references.pmid: 6874643
references.citation: Appl Environ Microbiol. 2009 Jun;75(11):3580-5
references.pmid: 19346351
references.citation: Appl Environ Microbiol. 2012 Jun;78(11):4062-4
references.pmid: 22447603
references.citation: Appl Microbiol Biotechnol. 2009 Apr;82(6):1115-22
references.pmid: 19214497
references.citation: J Bacteriol. 1984 Oct;160(1):273-8
references.pmid: 6480556
references.citation: Microbiology (Reading). 2012 Jan;158(Pt 1):293-300
references.pmid: 22016573
references.citation: Appl Environ Microbiol. 2008 Aug;74(15):4776-8
references.pmid: 18539801
references.citation: J Bacteriol. 1991 Jul;173(14):4411-6
references.pmid: 2066338
references.citation: J Bacteriol. 2001 Aug;183(15):4509-16
references.pmid: 11443085
references.citation: Arch Microbiol. 1984 May;138(1):44-8
references.pmid: 6742956
references.citation: Structure. 2001 Aug;9(8):725-38
references.pmid: 11587647
references.citation: J Bacteriol. 1975 Jul;123(1):242-7
references.pmid: 1141195
references.citation: Curr Opin Biotechnol. 2011 Apr;22(2):143-9
references.pmid: 21211959
references.citation: Anal Biochem. 1991 Jun;195(2):308-12
references.pmid: 1750685
references.citation: J Bacteriol. 2002 Nov;184(22):6351-6
references.pmid: 12399505
references.citation: J Appl Microbiol. 2006 Oct;101(4):903-12
references.pmid: 16968302
references.citation: Arch Microbiol. 1980 Apr;125(3):209-14
references.pmid: 7377904
references.citation: Int J Food Microbiol. 2010 Dec 15;144(2):270-9
references.pmid: 21035223
references.citation: Int J Food Microbiol. 1997 Apr 29;36(1):1-29
references.pmid: 9168311
references.citation: Int J Food Microbiol. 1991 Feb;12(2-3):133-40
references.pmid: 1904757
references.citation: Int J Food Microbiol. 2012 Apr 2;155(1-2):51-9
references.pmid: 22326142
references.citation: Appl Environ Microbiol. 1994 Jul;60(7):2627-36
references.pmid: 16349339