title: Differential propensity of citrate- and polyethylene glycol-coated silver nanoparticles to bovine hemoglobin.
abstract: Propensity of two different silver nanoparticles (Ag-NPs) to bovine hemoglobin (BHb) was investigated by means of spectroscopic methods. We have combined spectrophotometric and calorimetric methods to show that there is no significant interaction between citrate-coated Ag-NPs and BHb at physiological pH and 20°C. However, our previous results show that polyethylene glycol-coated Ag-NPs strongly bind to Hb and effect on the secondary and tertiary structures of BHb. Thus, a suitable surface coating and modification of surface charge would increase the NPs safety and reduce adverse biological responses.
authors.affiliation: Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Zolghadri
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran saboury@ut.ac.ir.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
authors.affiliation: Molecular and Cell Biology Department, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran.
authors.forename: Maliheh Sadat
authors.initials: MS
authors.lastname: Atri
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: A A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
mesh_terms: D000818:Animals; D002417:Cattle; D019343:Citric Acid / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000378:metabolism; D011485:Protein Binding; D012834:Silver / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D006454:Hemoglobins; D019343:Citric Acid; D012834:Silver; D011092:Polyethylene Glycols
keywords: Citrate-coated silver nanoparticles; circular dichroism; fluorescence; hemoglobin; nanoparticle safety; titration calorimetry