title: Biomechanical phenotyping of central arteries in health and disease: advantages of and methods for murine models.
abstract: The stiffness and structural integrity of the arterial wall depends primarily on the organization of the extracellular matrix and the cells that fashion and maintain this matrix. Fundamental to the latter is a delicate balance in the continuous production and removal of structural constituents and the mechanical state in which such turnover occurs. Perturbations in this balance due to genetic mutations, altered hemodynamics, or pathological processes result in diverse vascular phenotypes, many of which have yet to be well characterized biomechanically. In this paper, we emphasize the particular need to understand regional variations in the biaxial biomechanical properties of central arteries in health and disease and, in addition, the need for standardization in the associated biaxial testing and quantification. As an example of possible experimental methods, we summarize testing protocols that have evolved in our laboratory over the past 8 years. Moreover, we note advantages of a four fiber family stress-stretch relation for quantifying passive biaxial behaviors, the use of stored energy as a convenient scalar metric of the associated material stiffness, and the utility of appropriate linearizations of the nonlinear, anisotropic relations both for purposes of comparison across laboratories and to inform computational fluid-solid-interaction models. We conclude that, notwithstanding prior advances, there remain many opportunities to advance our understanding of arterial mechanics and mechanobiology, particularly via the diverse genetic, pharmacological, and surgical models that are, or soon will be, available in the mouse.
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ferruzzi
authors.forename: M R
authors.initials: MR
authors.lastname: Bersi
authors.forename: J D
authors.initials: JD
authors.lastname: Humphrey
mesh_terms: D000818:Animals; D001158:Arteries / Q000502:physiology*; D001696:Biomechanical Phenomena; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D010641:Phenotype
references.citation: Math Med Biol. 2010 Jun;27(2):129-55
references.pmid: 19592484
references.citation: J Hypertens. 2009 Oct;27(10):2010-21
references.pmid: 19584753
references.citation: J Biomech Eng. 1986 May;108(2):189-92
references.pmid: 3079517
references.citation: J Biomech Eng. 2009 Oct;131(10):101015
references.pmid: 19831485
references.citation: J Biomech Eng. 2004 Dec;126(6):815-22
references.pmid: 15796340
references.citation: Br J Pharmacol. 2007 Oct;152(3):305-12
references.pmid: 17641673
references.citation: Int J Eng Sci. 2010 Nov 1;48(11):1357-1372
references.pmid: 21218158
references.citation: Lancet. 2005 Apr 30-May 6;365(9470):1577-89
references.pmid: 15866312
references.citation: Med Clin North Am. 2009 May;93(3):583-604, Table of Contents
references.pmid: 19427493
references.citation: Thromb Res. 2011 Oct;128(4):317-24
references.pmid: 21843900
references.citation: Atherosclerosis. 2012 Oct;224(2):355-62
references.pmid: 22921425
references.citation: Ann Biomed Eng. 1999 Jul-Aug;27(4):459-68
references.pmid: 10468230
references.citation: Cardiovasc Res. 2011 Nov 1;92(2):287-95
references.pmid: 21730037
references.citation: J Vasc Res. 2011;48(6):495-504
references.pmid: 21832839
references.citation: Am J Physiol Heart Circ Physiol. 2002 Nov;283(5):H1829-37
references.pmid: 12384460
references.citation: J Biomech Eng. 2011 May;133(5):051009
references.pmid: 21599100
references.citation: J Physiol. 2010 Oct 15;588(Pt 20):3971-82
references.pmid: 20807791
references.citation: Nat Rev Cardiol. 2010 Aug;7(8):442-9
references.pmid: 20657613
references.citation: Am J Physiol. 1978 May;234(5):H542-51
references.pmid: 645919
references.citation: Cell Tissue Res. 2012 Jan;347(1):267-77
references.pmid: 22105919
references.citation: J Histochem Cytochem. 2008 Apr;56(4):359-70
references.pmid: 18071063
references.citation: J Biomech. 2009 Jan 5;42(1):1-8
references.pmid: 19070860
references.citation: Am J Physiol. 1979 Nov;237(5):H620-31
references.pmid: 495769
references.citation: J Biomech. 2011 Sep 23;44(14):2532-7
references.pmid: 21851943
references.citation: Biophys J. 2012 Jun 20;102(12):2916-25
references.pmid: 22735542
references.citation: Am J Physiol Heart Circ Physiol. 2003 Dec;285(6):H2614-22
references.pmid: 14613915
references.citation: Circ Res. 2009 May 22;104(10):1217-24
references.pmid: 19372465
references.citation: J Biomech. 2006;39(13):2347-54
references.pmid: 16872617
references.citation: Am J Physiol Heart Circ Physiol. 2010 Dec;299(6):H1875-83
references.pmid: 20852047
references.citation: Physiol Rev. 2009 Jul;89(3):957-89
references.pmid: 19584318
references.citation: Am J Physiol Heart Circ Physiol. 2005 Sep;289(3):H1209-17
references.pmid: 15863465
references.citation: J Biomech Eng. 2004 Dec;126(6):787-95
references.pmid: 15796337
references.citation: Am J Hypertens. 2011 Jan;24(1):5-17
references.pmid: 20940710
references.citation: Med Eng Phys. 2011 Dec;33(10):1262-9
references.pmid: 21742539
references.citation: Anat Rec. 2000 Jan 1;258(1):1-14
references.pmid: 10603443
references.citation: Atherosclerosis. 2012 Aug;223(2):314-20
references.pmid: 22770991
references.citation: Biomech Model Mechanobiol. 2013 Jun;12(3):497-510
references.pmid: 22790326
references.citation: J R Soc Interface. 2012 Sep 7;9(74):2047-58
references.pmid: 22491975
references.citation: J Biomech. 2009 Mar 11;42(4):524-30
references.pmid: 19159887
references.citation: Ann Biomed Eng. 2007 Apr;35(4):530-45
references.pmid: 17285364
references.citation: J Theor Biol. 2006 Jan 21;238(2):290-302
references.pmid: 16043190
references.citation: Rejuvenation Res. 2008 Feb;11(1):97-112
references.pmid: 18173368
references.citation: Int J Adv Eng Sci Appl Math. 2013 Dec 1;4(4):228-240
references.pmid: 24944461
references.citation: J Biomech. 2008 Aug 28;41(12):2618-27
references.pmid: 18684458
references.citation: Biorheology. 1973 Jun;10(2):139-55
references.pmid: 4728631
references.citation: Ann Biomed Eng. 2008 May;36(5):685-99
references.pmid: 18299987
references.citation: J Appl Physiol (1985). 2007 Aug;103(2):664-72
references.pmid: 17525297
references.citation: Comput Methods Biomech Biomed Engin. 2010 Dec;13(6):749-63
references.pmid: 20221934
references.citation: J Pathol. 2011 May;224(1):33-44
references.pmid: 21432852
references.citation: Ann Biomed Eng. 2009 Jun;37(6):1093-104
references.pmid: 19350391
references.citation: J Biomech. 1986;19(5):351-8
references.pmid: 3733760
references.citation: Am J Physiol Renal Physiol. 2006 Jun;290(6):F1329-36
references.pmid: 16380460
references.citation: J Biomech. 2008 Nov 14;41(15):3213-8
references.pmid: 18842267
references.citation: Biorheology. 1977;14(4):181-201
references.pmid: 912047
references.citation: J Biomech Eng. 2010 Dec;132(12):121010
references.pmid: 21142324
references.citation: Front Physiol. 2012 Nov 12;3:420
references.pmid: 23162468
references.citation: Annu Rev Genomics Hum Genet. 2008;9:283-302
references.pmid: 18544034
references.citation: J Biomech. 2012 Mar 15;45(5):772-9
references.pmid: 22196971
references.citation: Cell Biochem Biophys. 2008;50(2):53-78
references.pmid: 18209957
references.citation: J Biomech Eng. 2001 Dec;123(6):638-41
references.pmid: 11783737
references.citation: Circ Res. 2007 Aug 31;101(5):512-22
references.pmid: 17641224
references.citation: J R Soc Interface. 2011 Mar 6;8(56):435-50
references.pmid: 20659928
references.citation: Am J Physiol Heart Circ Physiol. 2005 Nov;289(5):H2048-58
references.pmid: 16006541
references.citation: J Biomech. 1983;16(9):703-15
references.pmid: 6643542
references.citation: Am J Physiol. 1967 Dec;213(6):1532-44
references.pmid: 6075755
references.citation: Math Med Biol. 2010 Dec;27(4):343-71
references.pmid: 20484365
references.citation: Arterioscler Thromb Vasc Biol. 2009 Dec;29(12):2083-9
references.pmid: 19850904
references.citation: J Theor Biol. 2011 Jan 7;268(1):120-30
references.pmid: 20946904
references.citation: J Mech Behav Biomed Mater. 2012 Jan;5(1):116-28
references.pmid: 22100086
references.citation: Hypertension. 2010 May;55(5):1267-74
references.pmid: 20212272
references.citation: Comput Methods Appl Mech Eng. 2009 Sep 15;198(45-46):3583-3602
references.pmid: 20160923
references.citation: J R Soc Interface. 2012 Jun 7;9(71):1275-86
references.pmid: 22171063
references.citation: J Biomech. 2012 Jul 26;45(11):1924-7
references.pmid: 22698830
references.citation: Hypertension. 2008 Aug;52(2):195-200
references.pmid: 18541735
references.citation: Ann Biomed Eng. 2010 Dec;38(12):3605-17
references.pmid: 20614245
references.citation: FASEB J. 2000 Nov;14(14):2213-20
references.pmid: 11053242
references.citation: Am J Physiol. 1983 Feb;244(2):H298-303
references.pmid: 6824097
references.citation: Biomech Model Mechanobiol. 2010 Dec;9(6):749-62
references.pmid: 20354752