title: Using sequential fluorescence and genomic in situ hybridization (FISH and GISH) to distinguish the A and C genomes in Brassica napus.
abstract: We have developed a sequential procedure with fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) that enables us to distinguish between the A and C genomes in Brassica napus and to identify certain individual chromosomes or chromosome groups within a genome. Our modified GISH technique uses a repetitive sequence in addition to the whole genome in the blocking DNA, and it is effective on meiotic and mitotic cells present in the anther material that we use.
authors.affiliation: School of Biosciences, The University of Birmingham, Birmingham, UK.
authors.forename: Elaine C
authors.initials: EC
authors.lastname: Howell
authors.forename: Susan
authors.initials: S
authors.lastname: Armstrong
mesh_terms: D029688:Brassica napus / Q000235:genetics*; D018745:Genome, Plant; D023281:Genomics / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods