title: MicroRNA-200b targets CREB1 and suppresses cell growth in human malignant glioma.
abstract: MicroRNAs can coordinately repress multiple target genes and interfere with the biological functions of the cell, such as proliferation and apoptosis. In the present study, we report that miR-200b was downregulated in malignant glioma cell lines and specimens. Overexpression of miR-200b suppressed the proliferation and colony formation of glioma cells. An oncogene encoding cAMP responsive element-binding protein 1 (CREB1), which has been shown to be an important transcription factor involved in the proliferation, survival, and metastasis of tumor cells, was here confirmed as a direct target gene of miR-200b. CREB1 was also found to be present at a high level in human glioma tissues. This was inversely correlated with miR-200b expression. Ectopic expression of CREB1 attenuated the growth suppressive phenotypes of glioma cells caused by miR-200b. These results indicate that miR-200b targets the CREB1 gene and suppresses glioma cell growth, suggesting that miR-200b shows tumor-suppressive activity in human malignant glioma.
authors.affiliation: Affiliated Cancer Hospital, Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China. pengbiaopengbiao@msn.com
authors.forename: Biao
authors.initials: B
authors.lastname: Peng
authors.forename: Su
authors.initials: S
authors.lastname: Hu
authors.forename: Qinming
authors.initials: Q
authors.lastname: Jun
authors.forename: Dongdong
authors.initials: D
authors.lastname: Luo
authors.forename: Xun
authors.initials: X
authors.lastname: Zhang
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhao
authors.forename: Dan
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009368:Neoplasm Transplantation; D034622:RNA Interference; D047368:Tumor Burden
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C533896:MIRN200 microRNA, human; D035683:MicroRNAs
references.citation: Cancer Cell. 2005 Apr;7(4):351-62
references.pmid: 15837624
references.citation: Neurosci Lett. 2003 Jan 30;337(1):9-12
references.pmid: 12524159
references.citation: Biomed Pharmacother. 2012 Sep;66(6):397-402
references.pmid: 22795796
references.citation: Cancer Res. 2007 Sep 15;67(18):8699-707
references.pmid: 17875710
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2257-61
references.pmid: 16461460
references.citation: Genes Dev. 2008 Apr 1;22(7):894-907
references.pmid: 18381893
references.citation: Nat Rev Mol Cell Biol. 2010 Apr;11(4):252-63
references.pmid: 20216554
references.citation: Arch Neurol. 2010 Mar;67(3):279-83
references.pmid: 20212224
references.citation: Nat Cell Biol. 2008 May;10(5):593-601
references.pmid: 18376396
references.citation: J Biol Chem. 2008 May 30;283(22):14910-4
references.pmid: 18411277
references.citation: Cancer Lett. 2011 Jan 1;300(1):10-9
references.pmid: 20965651
references.citation: PLoS One. 2010 Oct 04;5(10):
references.pmid: 20957176
references.citation: Nat Rev Mol Cell Biol. 2001 Aug;2(8):599-609
references.pmid: 11483993
references.citation: Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4459-64
references.pmid: 15753290
references.citation: Clin Cancer Res. 2009 Aug 15;15(16):5060-72
references.pmid: 19671845
references.citation: N Engl J Med. 2005 Oct 27;353(17):1768-71
references.pmid: 16251533
references.citation: Endocr Relat Cancer. 2010 Dec 21;18(1):85-95
references.pmid: 21051560
references.citation: Mol Pharmacol. 2009 Jun;75(6):1374-9
references.pmid: 19270061
references.citation: Mol Cell Biochem. 2010 Nov;344(1-2):261-6
references.pmid: 20683643
references.citation: EMBO Rep. 2008 Jun;9(6):582-9
references.pmid: 18483486
references.citation: Nature. 2005 Jun 9;435(7043):834-8
references.pmid: 15944708
references.citation: Annu Rev Biochem. 1999;68:821-61
references.pmid: 10872467
references.citation: Mol Cancer Ther. 2010 Aug;9(8):2265-75
references.pmid: 20647341
references.citation: Haematologica. 2007 Oct;92(10):1435-7
references.pmid: 18024382
references.citation: Nat Rev Cancer. 2006 Nov;6(11):857-66
references.pmid: 17060945
references.citation: Int J Cancer. 2010 Dec 1;127(11):2520-9
references.pmid: 20162574