title: IGF2 expression in blood is not associated with its imprinting status in healthy pregnant Chinese women.
abstract: Loss of Imprinting (LOI) of IGF2 and over-expressed IGF2 are associated with tumorigenesis. Our previous epidemiological study found a relatively high frequency of IGF2 LOI in healthy mid-gestation pregnant women. The aim of this study is to determine whether the expression of IGF2 is associated with its imprinting status in healthy Chinese pregnant women. The IGF2 imprinting status of 300 pregnant women was analyzed. 20 cases of IGF2 LOI and 20 cases of IGF2 retention of imprinting (ROI) were selected randomly for IGF2 expression analysis. The expression pattern of IGF2 between the group with IGF2 ROI and group with IGF2 LOI in healthy Chinese pregnant women was evaluated by real time PCR and western blot. The result showed no significant differences between IGF2 ROI and LOI groups in mRNA and protein levels. These results imply that IGF2 imprinting status has no obvious impact on its expression. There may be some unknown important factors other than imprinting status driving IGF2 expression.
authors.affiliation: Department of Reproductive Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Ru-Fei
authors.forename: Liu
authors.initials: L
authors.lastname: Xue-Qing
authors.forename: Wang
authors.initials: W
authors.lastname: Ying-Xiong
authors.forename: Chen
authors.initials: C
authors.lastname: Xue-Mei
authors.forename: Ding
authors.initials: D
authors.lastname: Yu-Bin
authors.forename: He
authors.initials: H
authors.lastname: Jun-Lin
mesh_terms: D000328:Adult; D002681:China; D005260:Female; D018392:Genomic Imprinting; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II