title: Analysis of meiotic protein complexes from Arabidopsis and Brassica using affinity-based proteomics.
abstract: The application of proteomics techniques to the study of plant meiosis has the potential to make a valuable contribution to our understanding of the molecular events underpinning meiotic processes. Here we describe the preparation of meiotic protein complexes from Arabidopsis thaliana and its close crop relative, Brassica oleracea, by co-immunoprecipitation for in-solution analysis by tandem mass spectrometry (MS/MS). Early results using these techniques have proved encouraging, enabling the identification of candidate AtASY1-interacting proteins in A. thaliana and providing evidence of an in planta interaction between BoASY1 and BoASY3 in B. oleracea. The detection of phospho-modified peptides of BoASY1 and BoASY3 suggests that this approach may be useful for studying meiotic protein modification events.
authors.affiliation: School of Biosciences, The University of Birmingham, Birmingham, UK.
authors.forename: Kim
authors.initials: K
authors.lastname: Osman
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Roitinger
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yang
authors.forename: Susan
authors.initials: S
authors.lastname: Armstrong
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.forename: F Chris H
authors.initials: FC
authors.lastname: Franklin
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001937:Brassica / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008540:Meiosis; D046912:Multiprotein Complexes / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D046912:Multiprotein Complexes; D010940:Plant Proteins