title: Star polymers with a cationic core prepared by ATRP for cellular nucleic acids delivery.
abstract: Poly(ethylene glycol) (PEG)-based star polymers with a cationic core were prepared by atom transfer radical polymerization (ATRP) for in vitro nucleic acid (NA) delivery. The star polymers were synthesized by ATRP of 2-(dimethylamino)ethyl methacrylate (DMAEMA) and ethylene glycol dimethacrylate (EGDMA). Star polymers were characterized by gel permeation chromatography, zeta potential, and dynamic light scattering. These star polymers were combined with either plasmid DNA (pDNA) or short interfering RNA (siRNA) duplexes to form polyplexes for intracellular delivery. These polyplexes with either siRNA or pDNA were highly effective in NA delivery, particularly at relatively low star polymer weight or molar ratios, highlighting the importance of NA release in efficient delivery systems.
authors.affiliation: Department of Chemistry, Center for Nucleic Acids Science and Technology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, United States.
authors.forename: Hong Y
authors.initials: HY
authors.lastname: Cho
authors.forename: Saadyah E
authors.initials: SE
authors.lastname: Averick
authors.forename: Eduardo
authors.initials: E
authors.lastname: Paredes
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wegner
authors.forename: Amram
authors.initials: A
authors.lastname: Averick
authors.forename: Stefan
authors.initials: S
authors.lastname: Jurga
authors.forename: Subha R
authors.initials: SR
authors.lastname: Das
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Matyjaszewski
mesh_terms: D000818:Animals; D002412:Cations; D002460:Cell Line; D002850:Chromatography, Gel; D004331:Drosophila melanogaster / Q000166:cytology; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D008027:Light; D008156:Luciferases; D008689:Methacrylates / Q000737:chemistry*; D010957:Plasmids; D011092:Polyethylene Glycols / Q000737:chemistry*; D058105:Polymerization; D034741:RNA, Small Interfering / Q000235:genetics; D012542:Scattering, Radiation; D019076:Transgenes
chemical_list: D002412:Cations; D008689:Methacrylates; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols; C004919:ethylene dimethacrylate; D008156:Luciferases; C049840:2-(dimethylamino)ethyl methacrylate