title: Prevalence of contagious mastitis pathogens in bulk tank milk in Québec.
abstract: The objective of this study was to estimate the prevalence of mycoplasma, Staphylococcus aureus, and Streptococcus agalactiae in bulk tank milk (BTM) in Québec dairy herds. BTM was sampled 3 times a month in 117 randomly selected dairy herds. Samples were submitted for S. aureus, S. agalactiae, and mycoplasma and for direct mycoplasma detection by polymerase chain reaction (PCR). Mycoplasma spp. was identified at least once in 3 herds (2.6%) by primary culture and/or PCR and in 4 herds (3.4%) by enrichment culture and/or PCR. Staphylococcus aureus was isolated at least once in 99 (84.6%) and 112 (95.7%) herds in primary culture and after enrichment, respectively. Streptococcus agalactiae was isolated at least once in 9 (7.7%) and 10 (8.6%) herds in primary culture and after enrichment, respectively. Herd prevalence of mycoplasma was similar to that previously reported in Canada. Staphylococcus aureus is still by far the most important contagious mastitis pathogen.
authors.affiliation: Département des Sciences cliniques, Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, CP 5000, Saint-Hyacinthe, Québec J2S 7C6, Canada. david.francoz@umontreal.ca
authors.forename: David
authors.initials: D
authors.lastname: Francoz
authors.forename: Luc
authors.initials: L
authors.lastname: Bergeron
authors.forename: Marie
authors.initials: M
authors.lastname: Nadeau
authors.forename: Guy
authors.initials: G
authors.lastname: Beauchamp
mesh_terms: D000818:Animals; D002417:Cattle; D003612:Dairying; D005260:Female; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D009174:Mycoplasma / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D011792:Quebec / Q000453:epidemiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013292:Streptococcus agalactiae / Q000302:isolation & purification*
references.citation: J Dairy Sci. 2004 Apr;87(4):933-6
references.pmid: 15259227
references.citation: Cornell Vet. 1971 Jul;61(3):519-42
references.pmid: 4105470
references.citation: N Z Vet J. 2009 Feb;57(1):44-9
references.pmid: 19252542
references.citation: N Z Vet J. 2011 Mar;59(2):75-8
references.pmid: 21409733
references.citation: J Vet Diagn Invest. 2005 Nov;17(6):537-45
references.pmid: 16475511
references.citation: Acta Vet Scand. 1998;39(1):119-26
references.pmid: 9592952
references.citation: Vet Res. 1999 Sep-Oct;30(5):457-66
references.pmid: 10543380
references.citation: Vet J. 2010 Dec;186(3):299-303
references.pmid: 19926318
references.citation: Can Vet J. 2006 Jun;47(6):567-72
references.pmid: 16808229
references.citation: J Am Vet Med Assoc. 1996 May 15;208(10):1705-8
references.pmid: 8641956
references.citation: Ann N Y Acad Sci. 2008 Dec;1149:300-2
references.pmid: 19120233
references.citation: Vet Res Commun. 2007 Aug;31 Suppl 1:225-7
references.pmid: 17682881
references.citation: Prev Vet Med. 2010 Oct 1;97(1):20-8
references.pmid: 20696486
references.citation: J Dairy Sci. 2001 Jul;84(7):1640-5
references.pmid: 11467814
references.citation: J Vet Med B Infect Dis Vet Public Health. 2003 Jun;50(5):235-40
references.pmid: 12864899
references.citation: Am J Vet Res. 2000 Sep;61(9):1092-8
references.pmid: 10976742
references.citation: Nihon Juigaku Zasshi. 1969 Apr;31(2):83-93
references.pmid: 5817669
references.citation: Vet Clin North Am Food Anim Pract. 2003 Mar;19(1):199-221
references.pmid: 12682943
references.citation: Vet Microbiol. 2010 Jul 29;144(1-2):210-3
references.pmid: 20053506
references.citation: Adv Vet Sci Comp Med. 1981;25:121-57
references.pmid: 7034498
references.citation: Vet Clin North Am Food Anim Pract. 1993 Nov;9(3):475-87
references.pmid: 8242453
references.citation: J Dairy Sci. 2004 Oct;87(10):3561-73
references.pmid: 15377636
references.citation: J Am Vet Med Assoc. 2003 Oct 15;223(8):1163-6
references.pmid: 14584748
references.citation: J Am Vet Med Assoc. 2009 Sep 15;235(6):749-54
references.pmid: 19751174
references.citation: Nihon Juigaku Zasshi. 1969 Dec;31(6):313-24
references.pmid: 5392349
references.citation: Vet Rec. 2001 May 5;148(18):567-72
references.pmid: 11370882
references.citation: J Vet Med B Infect Dis Vet Public Health. 2005 May;52(4):153-60
references.pmid: 16000109
references.citation: Vet J. 2002 Sep;164(2):116-28
references.pmid: 12359466
references.citation: Vet Clin North Am Food Anim Pract. 2003 Mar;19(1):75-92, vi
references.pmid: 12682936
references.citation: J Am Vet Med Assoc. 1986 Aug 15;189(4):442-5
references.pmid: 3531123
references.citation: Vet Microbiol. 1999 Mar 12;65(3):185-94
references.pmid: 10189193
references.citation: J Dairy Res. 2009 May;76(2):129-36
references.pmid: 19121233
references.citation: J Dairy Sci. 2004 Aug;87(8):2433-41
references.pmid: 15328265
references.citation: Mol Cell Probes. 1998 Jun;12(3):161-9
references.pmid: 9664578