title: Two-dimensional visualization of multicolor FISH-generated data as a helpful tool for the analysis and understanding of cytogenetic and chromosomal alterations in melanocytic lesions.
abstract: For the evaluation of data generated by multicolor fluorescence in situ hybridization (FISH), we present here a synergistic approach that integrates the 3 most commonly used numerical algorithms in conjunction with 2 newly devised graphic tools for data visualization, namely "signal curves" and "rhombic heat maps." These two graphic tools provide information additional to the currently used numerical algorithms and thus facilitate the recognition and compensation of inherent errors that occur with the numerical method.
authors.affiliation: Department of Dermatology, Zürich University Hospital, Zürich, Switzerland. katrin.kerl@usz.ch
authors.forename: Katrin
authors.initials: K
authors.lastname: Kerl
authors.forename: Leo
authors.initials: L
authors.lastname: Schärer
authors.forename: Raphael
authors.initials: R
authors.lastname: Hesse
authors.forename: Benedikt
authors.initials: B
authors.lastname: Hesse
authors.forename: Gabriela
authors.initials: G
authors.lastname: Palmedo
authors.forename: Luis
authors.initials: L
authors.lastname: Requena
authors.forename: Thomas
authors.initials: T
authors.lastname: Wiesner
authors.forename: Heinz
authors.initials: H
authors.lastname: Kutzner
mesh_terms: D000465:Algorithms; D002869:Chromosome Aberrations; D003116:Color; D055028:Comparative Genomic Hybridization; D003582:Cytogenetics / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008545:Melanoma / Q000235:genetics*; D009508:Nevus, Pigmented / Q000235:genetics*; D012878:Skin Neoplasms / Q000235:genetics*