title: Enzyme-assisted extraction of bioactive compounds from ginger (Zingiber officinale Roscoe).
abstract: Ginger (Zingiber officinale R.) is a popular spice used in various foods and beverages. 6-Gingerol is the major bioactive constituent responsible for the antiinflammatory, antitumour and antioxidant activities of ginger. The effect of application of α-amylase, viscozyme, cellulase, protease and pectinase enzymes to ginger on the oleoresin yield and 6-gingerol content has been investigated. Pre-treatment of ginger with α-amylase or viscozyme followed by extraction with acetone afforded higher yield of oleoresin (20%±0.5) and gingerol (12.2%±0.4) compared to control (15%±0.6 oleoresin, 6.4%±0.4 gingerol). Extraction of ginger pre-treated with enzymes followed by extraction with ethanol provided higher yield of gingerol (6.2-6.3%) than the control (5.5%) with comparable yields of the oleoresin (31-32%). Also, ethanol extract of cellulase pre-treated ginger had the maximum polyphenol content (37.5 mg/g). Apart from 6-gingerol, 6-paradol along with 6- and 8-methyl shogaols were the other important bio-active constituents in the oleoresin from cellulase-treated ginger.
authors.affiliation: Department of Plantation Products, Spices and Flavour Technology, CSIR-Central Food Technological Research Institute, Mysore 570 020, India.
authors.forename: K L
authors.initials: KL
authors.lastname: Nagendra chari
authors.forename: D
authors.initials: D
authors.lastname: Manasa
authors.forename: P
authors.initials: P
authors.lastname: Srinivas
authors.forename: H B
authors.initials: HB
authors.lastname: Sowbhagya
mesh_terms: D002396:Catechols / Q000032:analysis / Q000302:isolation & purification*; D002480:Cellulase / Q000737:chemistry*; D005591:Chemical Fractionation; D005233:Fatty Alcohols / Q000032:analysis / Q000302:isolation & purification*; D020939:Zingiber officinale / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; D009097:Multienzyme Complexes; D010936:Plant Extracts; C578134:Viscozyme L, Aspergillus; C018620:oleoresins; C007845:gingerol; D000516:alpha-Amylases; D002480:Cellulase