title: In vitro antimicrobial and brine shrimp lethality of Allophylus cobbe L.
abstract: Plants are rich source of pharmacologically active agents, which could be explored in disease management. Methanol, ethanol, and petroleum ether extracts of the whole plant of Allophylus cobbe L. were evaluated for antimicrobial and cytotoxic activities. In vitro antimicrobial sensitivity by disk diffusion method was conducted against four Gram-positive and seven Gram-negative pathogenic bacteria and seven fungi. In the antibacterial and antifungal sensitivity tests, growth inhibition was found to be within the range of 10.0-17.67 mm. Strong zone of inhibition by the ethanol extract of A. cobbe (EEAC) was found against Trichophyton spp. With some exceptions, a mild to strong antimicrobial activity was observed in this study. Significant minimum inhibitory concentration (MIC; 15.625 μg/ml) was found against Trichophyton spp. Other detected MICs were within the range of 31.25-125 μg/ml. The petroleum ether extract of the plant exhibited strong cytotoxicity in the brine shrimp lethality bioassay test.
authors.affiliation: Lecturer, Department of Pharmacy, Faculty of Science and Engineering, Southern University Bangladesh, Chittagong, Bangladesh.
authors.forename: Md Torequl
authors.initials: MT
authors.lastname: Islam
authors.forename: Md Abdun
authors.initials: MA
authors.lastname: Noor
authors.forename: Bijoy
authors.initials: B
authors.lastname: Karon
authors.forename: Rivelilson Mendes
authors.initials: RM
authors.lastname: de Freitas
keywords: Allophylus cobbe; antimicrobial; bioassay; brine shrimp lethality
references.citation: J Antimicrob Chemother. 2001 Jul;48 Suppl 1:5-16
references.pmid: 11420333
references.citation: Nat Prod Res. 2003 Jan;17(1):5-8
references.pmid: 12674135
references.citation: Am J Clin Pathol. 1966 Apr;45(4):493-6
references.pmid: 5325707
references.citation: Planta Med. 1982 May;45(1):31-4
references.pmid: 7100305