title: Chromatin immunoprecipitation for studying chromosomal localization of meiotic proteins in maize.
abstract: Chromatin immunoprecipitation (ChIP) is a method that allows identification of chromosomal sites occupied by specific proteins. In this technique, chromatin is extracted from cells, sheared, and, using a specific antibody, enriched in fragments that contain a protein of interest. Genomic location of the protein can then be identified by hybridization of the resulting DNA to tiling microarrays or by sequencing. Thanks to advances in high-throughput sequencing methods, studying protein localization using ChIP has become possible even in species with relatively large genomes. Here, we describe a ChIP protocol that we developed to examine localization of meiotic proteins in maize.
authors.affiliation: Cornell University, Ithaca, NY, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Gaganpreet
authors.initials: G
authors.lastname: Sidhu
authors.forename: Wojciech P
authors.initials: WP
authors.lastname: Pawlowski
mesh_terms: D002843:Chromatin / Q000302:isolation & purification / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D032461:Chromosomes, Plant; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D008540:Meiosis; D010940:Plant Proteins / Q000378:metabolism*; D013010:Sonication; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D010940:Plant Proteins