title: Proteomic analysis of a twin-arginine translocation-deficient mutant unravel its functions involved in stress adaptation and virulence in fish pathogen Edwardsiella tarda.
abstract: Edwardsiella tarda is a Gram-negative, facultative aerobic pathogen which infects multifarious hosts including fish, amphibians and human beings. A twin-arginine translocation (Tat) gene cluster important for high-salt tolerance in E. tarda was identified previously. Here the genetic structure and pleiotropic roles of the Tat system in physiological adaptation of the bacterium were further characterized. Functional analysis indicated that tatD was not required for Tat export process and tatE might be an allelic gene of tatA in the bacterium. The results showed that disruption in the Tat system did not affect the morphology and biofilm formation in E. tarda, but did affect motility, hemagglutination, cell aggregation and infection of eukaryotic cells (e.g. macrophage J774a). Comparative proteomics analysis of subcellular proteins using two-dimensional gel electrophoresis and a qualitative shotgun protein sequencing method were implemented to identify proteins differentially expressed in E. tarda EIB202 vs. ∆tatABCD. The results revealed a large repertoire of differentially expressed proteins (n = 61), shedding light on the Tat system associated with virulence and stress-associated processes in E. tarda.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Wang
authors.forename: Qiyao
authors.initials: Q
authors.lastname: Wang
authors.forename: Minjun
authors.initials: M
authors.lastname: Yang
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics; D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D018441:Biofilms; D001692:Biological Transport / Q000235:genetics; D002460:Cell Line; D020609:Edwardsiella tarda / Q000472:pathogenicity* / Q000502:physiology*; D005393:Fish Diseases / Q000382:microbiology; D005399:Fishes / Q000382:microbiology; D006384:Hemagglutination / Q000235:genetics; D026901:Membrane Transport Proteins / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D009154:Mutation; D010641:Phenotype; D040901:Proteomics / Q000379:methods; D013312:Stress, Physiological; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
chemical_list: D026901:Membrane Transport Proteins