title: An easy, quick and cheap high-throughput method for yeast DNA extraction from microwell plates.
abstract: We herein present a high-throughput and cheap method for yeast DNA isolation in a 96 well microplate. About 1500 yeast isolates can be processed within one working day and final DNA concentrations are suitable for direct application in PCR-based molecular typing methods.
authors.affiliation: Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Braga 4710-057, Portugal. drumondeneves@gmail.com
authors.forename: J
authors.initials: J
authors.lastname: Drumonde-Neves
authors.forename: E
authors.initials: E
authors.lastname: Vieira
authors.forename: M T
authors.initials: MT
authors.lastname: Lima
authors.forename: I
authors.initials: I
authors.lastname: Araujo
authors.forename: M
authors.initials: M
authors.lastname: Casal
authors.forename: D
authors.initials: D
authors.lastname: Schuller
mesh_terms: D004271:DNA, Fungal / Q000302:isolation & purification*; D008828:Microbiological Techniques / Q000191:economics / Q000379:methods*; D008967:Molecular Biology / Q000191:economics / Q000379:methods*; D058889:Molecular Typing / Q000379:methods; D016533:Mycological Typing Techniques / Q000379:methods; D013048:Specimen Handling / Q000191:economics / Q000379:methods*; D013997:Time Factors; D015003:Yeasts / Q000235:genetics*
chemical_list: D004271:DNA, Fungal