title: Bio-nanocapsules for signal enhancement of alkaline phosphatase-linked immunosorbent assays.
abstract: The bio-nanocapsules displaying about 240 molecules of immunoglobulin G Fc-binding Z domains (ZZ-BNCs) enhanced the signals of enzyme-linked immunosorbent assay by tethering the Fc regions of secondary antibodies (Abs), which were eliminated using high-molecular mass enzymes (e.g., alkaline phosphatase). By way of optimizing the distance between enzymes and Abs, ZZ-BNCs improved sensitivity independently of enzymes.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Iijima
authors.forename: Mikako
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yoshimoto
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Niimi
authors.forename: Shun'ichi
authors.initials: S
authors.lastname: Kuroda
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000276:immunology; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D008081:Liposomes / Q000737:chemistry; D008970:Molecular Weight; D053769:Nanocapsules; D017434:Protein Structure, Tertiary
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008081:Liposomes; D053769:Nanocapsules; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase