title: [Peptide fragments of chemokine domain of fractalkine: effect on human monocyte migration].
abstract: Leukocyte chemotaxis to the area of tissue damage is mediated by chemokines. According to the primary structure, chemokines are divided into four families, fractalkine (CX3CL1) is the only one member of CX3C family and the only membrane-bound chemokine. Fractalkine molecule includes the extracellular N-terminal chemokine domain, mucin-like rod, the transmembrane and the intracellular domains. In membrane-bound state fractalkine has the properties of an adhesion molecule. Chemokine domain of fractalkine (CDF) is released from cell membrane by proteolysis, and this soluble form acts as a chemoattractant for leukocytes expressing fractalkine receptor CX3CR1. Fractalkine is involved in development of a number of pathological processes caused by inflammation, and therefore a search for fractalkine inhibitors is very important. For this purpose we identified several antigenic determinants--the fragments of CDF, and the following peptides were synthesized--P41-52 H-Leu-Glu-Thr-Arg-Gln-His-Arg-Leu-Phe-Cys-Ala-Asp-NH2, P53-60 H-Pro-Lys-Glu-Gln-Trp-Val-Lys-Asp-NH2 and P60-71 H-Asp-Ala-Met-Gln-His-Leu-Asp-Arg-Gln-Ala-Ala-Ala-NH2. The peptide effects on adhesion and migration of human peripheral blood monocytes expressing fractalkine receptors were investigated. In the presence of CDF and P41-52 we observed the increased adhesion and migration of monocytes compared with spontaneous values. Peptides P53-60 and P60-71 significantly inhibited monocyte adhesion and migration stimulated by CDF. Since the chemotactic activity of chemokines was shown to be dependent on their binding to glycosaminoglycans of the cell surface and extracellular matrix, the effect ofpeptides on the interaction of CDF with heparin was analyzed by ELISA. Peptide P41-52 competed with CDF for heparin binding, while peptides P53-60 and P60-71 had no significant activity.
authors.forename: N B
authors.initials: NB
authors.lastname: Kukhtina
authors.forename: T I
authors.initials: TI
authors.lastname: Aref'eva
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Ruleva
authors.forename: M V
authors.initials: MV
authors.lastname: Sidorova
authors.forename: A A
authors.initials: AA
authors.lastname: Az'muko
authors.forename: Zh D
authors.initials: ZhD
authors.lastname: Bespalova
authors.forename: T L
authors.initials: TL
authors.lastname: Krasnikova
mesh_terms: D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D054428:Chemokine CX3CL1 / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D006801:Humans; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology
chemical_list: D054428:Chemokine CX3CL1; D010446:Peptide Fragments