title: Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae.
abstract: MoHrip2, a novel effector protein from the pathogenic fungus Magnaporthe oryzae, was purified and crystallized using the sitting-drop vapour-diffusion method. Native crystals and selenomethionine-labelled crystals were obtained using 2.2 M ammonium sulfate as a precipitant. A native data set was collected to 2.0 Å resolution at 100 K using an in-house X-ray source and a selenomethionine-labelled data set containing anomalous signal was collected to 1.8 Å resolution at 100 K using a synchrotron source. Based on the anomalous signal generated from the Se atom, the MoHrip2 structure was successfully solved using the single-wavelength anomalous dispersion (SAD) method.
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, People's Republic of China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Liu
authors.forename: Xinqi
authors.initials: X
authors.lastname: Liu
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zeng
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D020082:Magnaporthe / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins
keywords: Magnaporthe oryzae; MoHrip2; SAD; effector protein; selenomethionine
references.citation: Plant Cell. 2011 Jan;23(1):4-15
references.pmid: 21278123
references.citation: Science. 2012 Jun 1;336(6085):1160-4
references.pmid: 22654057
references.citation: Science. 2011 Jul 29;333(6042):596-601
references.pmid: 21798943
references.citation: J Mol Biol. 2004 Jul 16;340(4):783-95
references.pmid: 15223320
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: Nucleic Acids Res. 1990 Oct 25;18(20):6069-74
references.pmid: 2235490
references.citation: Plant Cell. 2005 Nov;17(11):3190-202
references.pmid: 16214901
references.citation: Curr Opin Plant Biol. 2008 Aug;11(4):380-8
references.pmid: 18550418
references.citation: Nucleic Acids Res. 2004 Aug 10;32(14):e115
references.pmid: 15304544
references.citation: Appl Microbiol Biotechnol. 2012 Jan;93(1):191-201
references.pmid: 21691787
references.citation: Mol Plant Microbe Interact. 2006 Jul;19(7):711-24
references.pmid: 16838784
references.citation: Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10359-64
references.pmid: 19520828
references.citation: PLoS One. 2012;7(5):e37654
references.pmid: 22624059
references.citation: Science. 2009 May 8;324(5928):742-4
references.pmid: 19423812
references.citation: Nature. 2006 Nov 16;444(7117):323-9
references.pmid: 17108957
references.citation: Nature. 2004 Apr 15;428(6984):764-7
references.pmid: 15085136
references.citation: Curr Opin Immunol. 2008 Feb;20(1):10-6
references.pmid: 18206360
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: Plant Cell Environ. 2012 Dec;35(12):2104-20
references.pmid: 22591019
references.citation: Mol Microbiol. 2002 Aug;45(3):875-84
references.pmid: 12139631