title: Homologue of Sox10 in Misgurnus anguillicaudatus: sequence, expression pattern during early embryogenesis.
abstract: A number of genetic studies have established that Sox10 is a transcription factor associated with neurogenesis in vertebrates. We have isolated a homologue of Sox10 gene from the brain of Misgurnus anguillicaudatus by using homologous cloning and RACE method, designated as MaSox10b. The full-length cDNA of MaSox10b contained a 311 bp 5'UTR, a 312 bp 3'UTR and an ORF encoding a putative protein of 490 amino acids with a characteristic HMG-box DNA-binding domain of 79 amino acids (aa: 105-183). Phylogenetic tree shows that the MaSOX10b fits within the Sox10 clade and clusters firmly into Sox10b branches. During embryogenesis, MaSox10b was first detected in gastrulae stage. From somitogenesis stage and thereafter, distinct expression was observed in the medial neural tube, extending from the hindbrain through the posterior trunk. Taken together, these preliminary findings suggested that MaSox10b is highly conserved during vertebrate evolution and involved in a wide range of developmental processes including embryogenesis and neurogenesis.
authors.affiliation: Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, 46# East of Construction Road, Xinxiang, 453007, Henan, People's Republic of China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xia
authors.forename: Ping
authors.initials: P
authors.lastname: Nan
authors.forename: Linxia
authors.initials: L
authors.lastname: Zhang
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Sun
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Chang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D003531:Cypriniformes / Q000196:embryology* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D017403:In Situ Hybridization / Q000662:veterinary; D008969:Molecular Sequence Data; D054259:Neural Tube / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055757:SOXE Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D055757:SOXE Transcription Factors
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