title: A simple and sensitive quality control method of the anaerobic atmosphere for identification and antimicrobial susceptibility testing of anaerobic bacteria.
abstract: The maintenance of a strict anaerobic atmosphere is essential for the culture of strict anaerobic bacteria. We describe a simple and sensitive quality control method of the anaerobic atmosphere, based on the measurement of the zone diameter around a 5-μg metronidazole disk when testing an aerotolerant Clostridium perfringens strain. A zone diameter above 27 mm was indicative of acceptable anaerobic conditions.
authors.affiliation: Department of Clinical Microbiology, Odense University Hospital, Odense DK-5000, Denmark.
authors.forename: Tage
authors.initials: T
authors.lastname: Justesen
authors.forename: Ulrik Stenz
authors.initials: US
authors.lastname: Justesen
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*; D003016:Clostridium perfringens / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D000890:Anti-Infective Agents; D008795:Metronidazole