title: First isolation and genetic characterization of a Toxoplasma gondii strain from a symptomatic human case of congenital toxoplasmosis in Romania.
abstract: Very limited data exists on the genetic diversity of Toxoplasma gondii from Eastern Europe. We present the first Romanian case of symptomatic congenital toxoplasmosis in which the T. gondii strain was isolated after inoculation in mice of a cerebrospinal fluid sample from a living neonate. The T. gondii strain was genotyped with 15 microsatellite markers distributed on 10 of the 14 chromosomes of T. gondii. The strain had a type II genotype.
authors.affiliation: Department of Microbiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, 6 Louis Pasteur street, Cluj-Napoca 400349, Romania.
authors.forename: Carmen Anca
authors.initials: CA
authors.lastname: Costache
authors.forename: Horaţiu Alexandru
authors.initials: HA
authors.lastname: Colosi
authors.forename: Ligia
authors.initials: L
authors.lastname: Blaga
authors.forename: Adriana
authors.initials: A
authors.lastname: Györke
authors.forename: Anamaria Ioana
authors.initials: AI
authors.lastname: Paştiu
authors.forename: Ioana Alina
authors.initials: IA
authors.lastname: Colosi
authors.forename: Daniel
authors.initials: D
authors.lastname: Ajzenberg
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001681:Biological Assay; D015153:Blotting, Western; D002555:Cerebrospinal Fluid / Q000469:parasitology; D016054:DNA, Protozoan / Q000134:cerebrospinal fluid / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D060005:Genotyping Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000097:blood; D007231:Infant, Newborn; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000469:parasitology*; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D012383:Romania; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007136:Immunoglobulins
references.citation: PLoS Negl Trop Dis. 2010 Nov 02;4(11):e876
references.pmid: 21072237
references.citation: J Parasitol. 2010 Dec;96(6):1112-4
references.pmid: 21158618
references.citation: J Infect Dis. 2009 Apr 15;199(8):1155-67
references.pmid: 19265484
references.citation: Int J Parasitol. 2002 Jan;32(1):27-38
references.pmid: 11796120
references.citation: Clin Infect Dis. 2012 Jun;54(11):1595-605
references.pmid: 22499837
references.citation: J Infect Dis. 2002 Sep 1;186(5):684-9
references.pmid: 12195356
references.citation: Parasitol Int. 2009 Jun;58(2):193-5
references.pmid: 19567233
references.citation: mBio. 2011 Nov 08;2(6):e00228-11
references.pmid: 22068979
references.citation: Int J Parasitol. 2010 Feb;40(2):193-200
references.pmid: 19631651
references.citation: Vet Parasitol. 2009 Oct 14;164(2-4):335-9
references.pmid: 19556061
references.citation: J Clin Microbiol. 2010 Dec;48(12):4641-5
references.pmid: 20881166
references.citation: Future Microbiol. 2010 Jun;5(6):841-3
references.pmid: 20521929
references.citation: Infect Genet Evol. 2012 Mar;12(2):359-68
references.pmid: 22226702
references.citation: J Parasitol. 2008 Feb;94(1):68-71
references.pmid: 18372623
references.citation: Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13458-63
references.pmid: 22847430
references.citation: Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5844-9
references.pmid: 22431627
references.citation: J Clin Microbiol. 2006 Apr;44(4):1382-9
references.pmid: 16597866
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11423-8
references.pmid: 16849431
references.citation: Microbes Infect. 2003 Jun;5(7):685-90
references.pmid: 12787745
references.citation: Prenat Diagn. 2010 Sep;30(9):902-5
references.pmid: 20582922
references.citation: Microbes Infect. 2006 Jul;8(8):2206-12
references.pmid: 16797199
references.citation: Vet Parasitol. 2012 Aug 13;188(1-2):14-8
references.pmid: 22483557