title: Parthenolide attenuates LPS-induced activation of NF-κB in a time-dependent manner in rat myocardium.
abstract: Parthenolide (PTN), a selective nuclear factor kappa B (NF-κB) inhibitor, has been used extensively to inhibit NF-κB activation. The duration of the inhibitory effect of PTN on NF-κB in vivo remains unclear. This study was to determine whether a lipopolysaccharide (LPS) challenge 6, 12 and 24 h after the administration of PTN could activate NF-κB. Rats were devided into five groups. The rats in the PTN, PTN+LPS and DMSO groups were injected intraperitoneally with PTN or DMSO. After 6, 12 or 24 h, LPS was administered in LPS and PTN+LPS groups. The expressions of NF-κB p50, IκBα and p-IκBα were inhibited in both PTN and PTN+LPS group at end of 6 and 12 h and no effects at 24 h. In summary, myocardial NF-κB expression occurs 1 h after the administration of LPS. PTN blocks this effect given at 6 h and no inhibitory effect 24 h after administration in vivo.
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China;
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wu
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Shigang
authors.initials: S
authors.lastname: Qiao
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
keywords: lipopolysaccharide; myocardium; nuclear-factor-κB; parthenolide
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