title: Ultrasound bio-microscopic image segmentation for evaluation of zebrafish cardiac function.
abstract: Zebrafish can fully regenerate their myocardium after ventricular resection without evidence of scars. This extraordinary regenerative ability provides an excellent model system to study the activation of the regenerative potential for human heart tissue. In addition to the morphology, it is vital to understand the cardiac function of zebrafish. To characterize adult zebrafish cardiac function, an ultrasound biomicroscope (UBM) was customized for real-time imaging of the zebrafish heart (about 1 mm in diameter) at a resolution of around 37 μm. Moreover, we developed an image segmentation algorithm to track the cardiac boundary and measure the dynamic size of the zebrafish heart for further quantification of zebrafish cardiac function. The effectiveness and accuracy of the proposed segmentation algorithm were verified on a tissuemimicking phantom and in vivo zebrafish echocardiography. The quantitative evaluation demonstrated that the accuracy of the proposed algorithm is comparable to the manual delineation by experts.
authors.affiliation: Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Hong Kong SAR, China. eexwzhou@ust.kh
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yu
authors.forename: Weibao
authors.initials: W
authors.lastname: Qiu
authors.forename: Ching-Ling
authors.initials: CL
authors.lastname: Lien
authors.forename: K Kirk
authors.initials: KK
authors.lastname: Shung
authors.forename: Weichuan
authors.initials: W
authors.lastname: Yu
mesh_terms: D000465:Algorithms; D000818:Animals; D004452:Echocardiography / Q000379:methods*; D006321:Heart / Q000033:anatomy & histology* / Q000502:physiology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D033401:Microscopy, Acoustic / Q000379:methods*; D019047:Phantoms, Imaging; D015027:Zebrafish
references.citation: Science. 2002 Dec 13;298(5601):2188-90
references.pmid: 12481136
references.citation: Phys Med Biol. 1998 May;43(5):1241-54
references.pmid: 9623653
references.citation: Ultrasound Med Biol. 2008 Jan;34(1):31-9
references.pmid: 17825980
references.citation: IEEE Trans Biomed Eng. 2008 Aug;55(8):2039-49
references.pmid: 18632366
references.citation: IEEE Trans Image Process. 2001;10(2):266-77
references.pmid: 18249617
references.citation: Annu Rev Biomed Eng. 2005;7:223-53
references.pmid: 16004571
references.citation: Ultrasound Med Biol. 2011 Sep;37(9):1409-20
references.pmid: 21683512
references.citation: Am Heart J. 2006 Jun;151(6 Suppl):S30-9
references.pmid: 16777508
references.citation: Anat Rec. 2001 Sep 1;264(1):1-12
references.pmid: 11505366
references.citation: IEEE Trans Med Imaging. 2006 Aug;25(8):987-1010
references.pmid: 16894993
references.citation: PLoS Biol. 2006 Aug;4(8):e260
references.pmid: 16869712
references.citation: IEEE Trans Ultrason Ferroelectr Freq Control. 1999;46(1):82-96
references.pmid: 18238401
references.citation: Med Image Anal. 2003 Sep;7(3):353-67
references.pmid: 12946474
references.citation: IEEE Trans Ultrason Ferroelectr Freq Control. 2010 Oct;57(10):2284-92
references.pmid: 20889416
references.citation: Proc Natl Acad Sci U S A. 2003 Sep 30;100 Suppl 1:11889-95
references.pmid: 12909711
references.citation: Med Image Anal. 2006 Apr;10(2):162-77
references.pmid: 16165394
references.citation: Ultrasound Med Biol. 2002 Sep;28(9):1137-43
references.pmid: 12401383
references.citation: Zebrafish. 2006;3(4):431-9
references.pmid: 18377223