title: Ebola virus VP35 induces high-level production of recombinant TPL-2-ABIN-2-NF-κB1 p105 complex in co-transfected HEK-293 cells.
abstract: Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)-ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]-NF-κB1 p105 complex. VP35 also increased expression of other co-transfected proteins, suggesting that VP35 could be employed generally to boost recombinant protein production by HEK-293 cells.
authors.affiliation: Division of Immune Cell Biology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Gantke
authors.forename: Sabrina
authors.initials: S
authors.lastname: Boussouf
authors.forename: Julia
authors.initials: J
authors.lastname: Janzen
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Morrice
authors.forename: Steven
authors.initials: S
authors.lastname: Howell
authors.forename: Elke
authors.initials: E
authors.lastname: Mühlberger
authors.forename: Steven C
authors.initials: SC
authors.lastname: Ley
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000235:genetics; D029043:Ebolavirus / Q000235:genetics / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D020930:MAP Kinase Kinase Kinases / Q000096:biosynthesis* / Q000235:genetics; D046912:Multiprotein Complexes / Q000096:biosynthesis / Q000235:genetics; D052002:NF-kappa B p50 Subunit / Q000096:biosynthesis* / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D015854:Up-Regulation / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D046912:Multiprotein Complexes; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; C434062:TNIP2 protein, human; C080980:VP35 protein, filovirus; D054334:Viral Regulatory and Accessory Proteins; D020930:MAP Kinase Kinase Kinases; C069937:MAP3K8 protein, human
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