title: Sphingobium sufflavum sp. nov., isolated from a freshwater lake.
abstract: A Gram-stain negative, rod-shaped, non-motile, non-spore-forming and aerobic bacterial strain, designated HL-25(T), was isolated and characterized in a taxonomic study using a polyphasic approach. Comparative analysis of the 16S rRNA gene sequences showed that the isolate constituted a distinct branch within the genus Sphingobium, showing the highest level of 16S rRNA gene sequence similarity to Sphingobium vulgare HU1-GD12(T) (96.6 %). The major fatty acids (>10%) of strain HL-25(T) were C(18:1)ω7c, C(16:0), summed feature 3 (comprising C(16:1)ω7c and/or C(16:1)ω6c) and C(18:0). The major cellular hydroxy fatty acid was C(14:0) 2-OH. The major isoprenoid quinone was Q-10 and the DNA G+C content was 63.8 mol%. The polar lipid profile consisted of a mixture of sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, diphosphatidylglycerol, an uncharacterized glycolipid, an uncharacterized aminophospholipid and four uncharacterized phospholipids. The polyamine pattern of strain HL-25(T) contained spermidine and putrescine. On the basis of these genotypic, chemotaxonomic and phenotypic data, strain HL-25(T) represents a novel species in the genus Sphingobium, for which the name Sphingobium sufflavum sp. nov. is proposed. The type strain is HL-25(T) ( =BCRC 80413(T) =KCTC 23953(T)).
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Shiau
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013624:Taiwan; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10