title: Circulating miR-583 and miR-663 Refer to ZHENG Differentiation in Chronic Hepatitis B.
abstract: Traditional Chinese medicine (TCM) ZHENG as the key pathological principle is to understand the human homeostasis and guide TCM treatment. Here, circulating microRNAs (miRNAs) were utilized to differentiate between ZHENGs including liver-gallbladder dampness-heat syndrome (LGDHS) and liver-kidney yin deficiency syndrome (LKYDS) in chronic hepatitis B (CHB). Sera samples of CHB patients with LGDHS (n = 35), LKYDS (n = 24), and healthy controls (Ctrls, n = 21) were analyzed by microarray and real-time RT-PCR. Receiver-operator characteristic (ROC) curves were established to evaluate the levels of serum miRNA for discriminating LGDHS and LKYDS. The target genes of miRNAs were predicted by TargetScan. Gene Ontology (GO) and pathways were analyzed using DAVID tool. The results showed that 22 miRNAs were differentially expressed between LGDHS and LKYDS (fold change >2.0 and P < 0.01). Circulating miR-583 and miR-663 were significantly higher (P < 0.001) in CHB patients with LGDHS than those with LKYDS and Ctrls. ROC curve analysis revealed that miR-583 and miR-663 were sensitive and specific enough to distinguish LGDHS from LKYDS. Pathway enrichment analysis indicated that 354 putative targets for miR-583 and 68 putative targets for miR-663 were mainly involved in Axon guidance, Neurotrophin, and MAPK signaling pathway. miR-583 and miR-663 may be potential markers for ZHENG differentiation in CHB.
authors.affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong, Shanghai 201203, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Guan
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Lu
authors.forename: Yi-Yang
authors.initials: YY
authors.lastname: Hu
authors.forename: Shuang
authors.initials: S
authors.lastname: Huang
authors.forename: Shi-Bing
authors.initials: SB
authors.lastname: Su
references.citation: Clin Sci (Lond). 2010 Apr 20;119(2):87-95
references.pmid: 20218970
references.citation: PLoS One. 2008 Sep 05;3(9):e3148
references.pmid: 18773077
references.citation: J Gastroenterol Hepatol. 2000 May;15 Suppl:E67-70
references.pmid: 10921385
references.citation: Nature. 1985 Oct 10-16;317(6037):489-95
references.pmid: 2995835
references.citation: Eur Heart J. 2010 Mar;31(6):659-66
references.pmid: 20159880
references.citation: Int J Hepatol. 2012;2012:328372
references.pmid: 23133759
references.citation: World J Gastroenterol. 2012 Oct 7;18(37):5188-96
references.pmid: 23066312
references.citation: Sci Signal. 2009 Dec 08;2(100):ra81
references.pmid: 19996457
references.citation: J Clin Invest. 2010 Jul;120(7):2379-94
references.pmid: 20577048
references.citation: J Clin Invest. 2013 Jan;123(1):164-78
references.pmid: 23202732
references.citation: J Clin Gastroenterol. 2004 Nov-Dec;38(10 Suppl 3):S158-68
references.pmid: 15602165
references.citation: Clin Chem. 2004 Mar;50(3):564-73
references.pmid: 14718398
references.citation: Exp Neurol. 2009 Oct;219(2):424-9
references.pmid: 19576215
references.citation: Evid Based Complement Alternat Med. 2012;2012:246538
references.pmid: 22927876
references.citation: Clin Chem. 2008 Mar;54(3):482-90
references.pmid: 18218722
references.citation: Biochem Biophys Res Commun. 2010 Mar 26;394(1):184-8
references.pmid: 20188071
references.citation: Dev Cell. 2006 Oct;11(4):441-50
references.pmid: 17011485
references.citation: Hepatology. 2001 Jan;33(1):148-58
references.pmid: 11124831
references.citation: J Int Med Res. 2009 May-Jun;37(3):662-7
references.pmid: 19589248
references.citation: Nat Cell Biol. 2007 Jun;9(6):654-9
references.pmid: 17486113
references.citation: Clin Chem. 2009 Nov;55(11):1977-83
references.pmid: 19745058
references.citation: J Ethnopharmacol. 2012 Jun 1;141(2):549-56
references.pmid: 21896324
references.citation: Nature. 2004 Sep 16;431(7006):350-5
references.pmid: 15372042
references.citation: Cell Res. 2008 Oct;18(10):997-1006
references.pmid: 18766170
references.citation: Clin Sci (Lond). 2011 Mar;120(5):183-93
references.pmid: 20815808
references.citation: Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10513-8
references.pmid: 18663219
references.citation: PLoS One. 2008;3(11):e3694
references.pmid: 19002258
references.citation: Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(23):2468-70
references.pmid: 18330233
references.citation: Lancet. 2008 Dec 6;372(9654):1938-40
references.pmid: 18930523