title: Bioaugmentation accelerates the shift of bacterial community structure against shock load: a case study of coking wastewater treatment by zeolite-sequencing batch reactor.
abstract: Bioaugmentation with degrading bacteria is an effective method to improve the treatment of refractory industrial wastewater; nevertheless there were controversial opinions about the fate of inoculated bacteria and microbial community dynamics. In this study, two lab-scale sequencing batch reactors filled with modified zeolite were used to treat a coking wastewater with pyridine and quinoline shock load, and a bacterial consortium containing three degrading strains was added in one reactor for bioaugmentation. During 120-day operation, the bioaugmented reactor removed over 99 % pyridine, 99 % quinoline, 85 % TOC, 65 % COD, and 95 % NO₃⁻--N with higher resistance to the shock load than the non-bioaugmented reactor. Based on the terminal restriction fragment length polymorphism of 16S rDNA, bacterial community diversity increased in the bioaugmented reactor. Principal component analysis revealed that, to cope with the shock load, the indigenous bacterial community recovered to the initial structure by acclimatizing itself constantly to the inhospitable environment; but bioaugmentation accelerated the shift of whole bacterial community, resulting in a far different structure from the initial one. Canonical correspondence analysis indicated that the environmental parameters of pyridine, quinoline, TOC, and NO₃⁻--N had close negative correlations with bioaugmentation; and NH₃--N and COD were the main parameters to impact on the bacterial community changes and treatment efficiency.
authors.affiliation: College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences (Ministry of Education), Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Donghui
authors.initials: D
authors.lastname: Wen
authors.forename: Cui
authors.initials: C
authors.lastname: Zhao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D001711:Biotransformation; D002244:Carbon / Q000032:analysis; D016172:DNA Fingerprinting; D009584:Nitrogen / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D011725:Pyridines / Q000378:metabolism; D015363:Quinolones / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology*; D014873:Water Pollutants / Q000378:metabolism*; D017641:Zeolites
chemical_list: D002244:Carbon; D009584:Nitrogen; D011725:Pyridines; D015363:Quinolones; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014873:Water Pollutants; D017641:Zeolites; C023666:pyridine