title: Biochemical and haematological assessment of toxic effects of the leaf ethanol extract of Petroselinum crispum (Mill) Nyman ex A.W. Hill (Parsley) in rats.
abstract: BACKGROUND
  Petroselinum crispum, a bright green biennial shrub is widely used traditionally as a food additive and herbal remedies for many ailments. This study therefore aimed to assess the toxic effects of its leaf extract using some biochemical, haematological parameters.
  
  
  METHODS
  The toxic effects were assessed by quantifying liver enzymes such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total serum protein and liver weight. Effects on haematological parameters were assessed by analysis of parked cell volume (PCV), red blood cell count (RBC), white blood cells (WBC) and haemoglobin (Hb) concentrations. Histopathological studies were done on the liver and kidneys.
  
  
  RESULTS
  The extract caused significant increase in serum activity of alanine amino transferase and blood urea nitrogen (BUN) levels at the dose of 1000 mg/kg. Other biochemical and haematological parameters were not affected at lower doses. Conversely, the liver weight was not affected after eight weeks of treatment at the dose levels studied. The organs obtained for pathological study, were structurally unchanged under histopathological evaluation at lower doses but inflammatory and necrotic features were observed at doses ≥ 1000 mg/kg.
  
  
  CONCLUSIONS
  The results indicate that the leaf ethanol extract of Petroselinum crispum was hepatotoxic and nephrotoxic at continued oral doses equal to or more than 1000 mg/kg, but no obvious toxicity when used at lower doses. Therefore, there should be caution in its administration to avoid overdosing and known interaction with some medications. In addition, the plant should be kept away from pets and domestic animals and should not be cultivated on soil irrigated with waste water due to their ability to bio-accumulate toxic metals.
authors.affiliation: Department of Pharmacology & Therapeutics, College of Health Sciences, Ladoke, Akintola University of Technology, Ogbomoso, Nigeria. awemann@yahoo.co.uk
authors.forename: Emmanuel Olorunju
authors.initials: EO
authors.lastname: Awe
authors.forename: S Olatunbosun
authors.initials: SO
authors.lastname: Banjoko
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001772:Blood Cell Count; D006454:Hemoglobins / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D028528:Petroselinum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006454:Hemoglobins; D010936:Plant Extracts; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase
references.citation: J Ethnopharmacol. 2002 Mar;79(3):353-7
references.pmid: 11849841
references.citation: Biol Trace Elem Res. 2012 Jun;146(3):369-75
references.pmid: 22147333
references.citation: J Clin Pathol. 1960 Mar;13:156-9
references.pmid: 13821779
references.citation: Clin Exp Dermatol. 1985 Jul;10(4):328-31
references.pmid: 4042407
references.citation: Phytother Res. 2006 Jul;20(7):531-7
references.pmid: 16685681
references.citation: J Food Prot. 1997 Jan;60(1):72-7
references.pmid: 10465045
references.citation: Am J Clin Pathol. 1957 Jul;28(1):56-63
references.pmid: 13458125
references.citation: Pharmacogn Mag. 2011 Apr;7(26):151-6
references.pmid: 21716923
references.citation: Semin Liver Dis. 2002;22(2):145-55
references.pmid: 12016546
references.citation: N Engl J Med. 1998 Oct 22;339(17):1217-27
references.pmid: 9780343
references.citation: Eur J Drug Metab Pharmacokinet. 2002 Jul-Sep;27(3):153-6
references.pmid: 12365194
references.citation: Proc Soc Exp Biol Med. 1960 Aug-Sep;104:751-4
references.pmid: 13741689
references.citation: Chem Pharm Bull (Tokyo). 2000 Jul;48(7):1039-44
references.pmid: 10923837
references.citation: Clin Chim Acta. 1971 Jan;31(1):87-96
references.pmid: 5544065
references.citation: Science. 1954 Jan 15;119(3081):100
references.pmid: 17840528