title: Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11.
abstract: The selective autophagy receptors Atg19 and Atg32 interact with two proteins of the core autophagic machinery: the scaffold protein Atg11 and the ubiquitin-like protein Atg8. We found that the Pichia pastoris pexophagy receptor, Atg30, also interacts with Atg8. Both Atg30 and Atg32 interactions are regulated by phosphorylation close to Atg8-interaction motifs. Extending this finding to Saccharomyces cerevisiae, we confirmed phosphoregulation for the mitophagy and pexophagy receptors, Atg32 and Atg36. Each Atg30 molecule must interact with both Atg8 and Atg11 for full functionality, and these interactions occur independently and not simultaneously, but rather in random order. We present a common model for the phosphoregulation of selective autophagy receptors.
authors.affiliation: Section of Molecular Biology, Division of Biological Sciences, University California, San Diego, California 92093-0322, USA.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Farré
authors.forename: Aaron
authors.initials: A
authors.lastname: Burkenroad
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Burnett
authors.forename: Suresh
authors.initials: S
authors.lastname: Subramani
mesh_terms: D000595:Amino Acid Sequence; D001343:Autophagy; D000071190:Autophagy-Related Protein 8 Family; D000071183:Autophagy-Related Proteins; D001665:Binding Sites; D016384:Consensus Sequence; D055786:Gene Knockout Techniques; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D063306:Mitophagy; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010843:Pichia / Q000235:genetics; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D011956:Receptors, Cell Surface / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: C434480:ATG19 protein, S cerevisiae; C113757:ATG8 protein, S cerevisiae; C502923:Atg11 protein, S cerevisiae; C543183:Atg32 protein, S cerevisiae; D000071190:Autophagy-Related Protein 8 Family; D000071183:Autophagy-Related Proteins; D008869:Microtubule-Associated Proteins; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins
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