title: Lipopolysaccharide structure of Helicobacter pylori serogroup O:3.
abstract: In this study, we describe a re-investigation of the lipopolysaccharide structure of Helicobacter pylori serogroup O:3. Application of NMR and MS approaches to the analysis of oligosaccharides obtained through degradation of LPS from H. pylori serogroup O:3 by various methods confirmed that its general architecture was identical to that of LPS from H. pylori strains 26695 and SS1 and followed a sequential linear assembly of the α-1,6-glucan, dd-heptan, and O-chain components. Additionally, MALDI-MS analysis demonstrated that a significant proportion of H. pylori serogroup O:3 LPS was terminated with α-1,6-glucan and was not further substituted by dd-heptan and the O-chain polysaccharide.
authors.affiliation: National Research Council Canada, 100 Sussex Drive, Ottawa, ON, Canada K1A 0R6.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Altman
authors.forename: Vandana
authors.initials: V
authors.lastname: Chandan
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
mesh_terms: D002240:Carbohydrate Sequence; D016480:Helicobacter pylori / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data
chemical_list: D008070:Lipopolysaccharides
keywords: DQCOSY; GLC; HMBC; HSQC; Helicobacter pylori; Hep; Hex; HexNAc; LPS; Le; Lewis; MALDI; MS; N-acetyl-hexosamine; NMR; NOESY; PEtN; Structure; TOCSY; double-quantum correlated spectroscopy; gas liquid chromatography; heptose; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; hexose; lipopolysaccharide; mass spectrometry; matrix-assisted laser desorption ionization; nuclear Overhauser enhancement spectroscopy; phosphoethanolamine; total correlated spectroscopy