title: Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects.
abstract: BACKGROUND
  The model of pulse plethysmograph using inhalational salbutamol 400 mcg is studied well to assess endothelium dependent vasodilation. Endothelial nitric oxide synthase (eNOS) gene polymorphism may influence the response to salbutamol in healthy subjects.
  
  
  OBJECTIVE
  To find the effect of polymorphisms 894G>T and -786T>C of eNOS gene on endothelium dependent vasodilation in healthy subjects.
  
  
  METHODS
  One hundred and two south Indian healthy subjects of either sex, aged between 18 to 35 years were recruited for the study. The digital volume pulse (DVP)was measured by pulse plethysmograph before and after salbutamol 400mcg inhalation. Three predose and five postdose recordings of DVP were measured. The average change in the DVP parameters namely reflection index (RI) and stiffness index (SI) were determined. The eNOS894G>T and -786T>C gene polymorphism were genotyped using polymerase chain reaction followed by restriction fragment length polymorphism. The percentage changes in RI and SI from predose baseline recordings were calculated and compared between the genotype groups.
  
  
  RESULTS
  The genotype and allele frequency of study subjects were in Hardy-Weinberg equilibrium. The changes in DVP parameters were not significantly different between the genotype groups.
  
  
  CONCLUSIONS
  eNOS polymorphism do not affect salbutamol evoked endothelium dependent vasodilation in the model of pulse plethysmograph in healthy subjects.
authors.affiliation: Pharmacogenomics Laboratory, Department of Pharmacology, JIPMER, Pondicherry, India.
authors.forename: Srinivasamurthy Suresh
authors.initials: SS
authors.lastname: Kumar
authors.forename: Annan Sudarsan Arun
authors.initials: AS
authors.lastname: Kumar
authors.forename: Ramamoorthy
authors.initials: R
authors.lastname: Padmapriya
authors.forename: Adithan
authors.initials: A
authors.lastname: Chandrasekaran
mesh_terms: D000280:Administration, Inhalation; D000293:Adolescent; D058666:Adrenergic beta-2 Receptor Agonists / Q000008:administration & dosage*; D000328:Adult; D000420:Albuterol / Q000008:administration & dosage*; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D010991:Plethysmography; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D014664:Vasodilation / Q000502:physiology*; D055815:Young Adult
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D052250:Nitric Oxide Synthase Type III; D000420:Albuterol
keywords: Digital volume pulse; eNOS; endothelium dependent vasodilation; polymorphism; pulse plethysmograph
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