title: Biodegradation of 2,4-dinitrophenol with laccase immobilized on nano-porous silica beads.
abstract: Many organic hazardous pollutants, including 2,4-dinitrophenol (2,4-DNP), which are water soluble, toxic, and not easily biodegradable make concerns for environmental pollution worldwide. In the present study, degradation of nitrophenols-contained effluents by using laccase immobilized on the nano-porous silica beads was evaluated. 2,4-DNP was selected as the main constituent of industrial effluents containing nitrophenols. The performance of the system was characterized as a function of pH, contact time, temperature, pollutant, and mediator concentrations. The laccase-silica beads were employed in a mixed-batch reactor to determine the degradation efficiency after 12 h of enzyme treatment. The obtained data showed that the immobilized laccase degraded more than 90% of 2,4-DNP within 12 h treatment. The immobilization process improved the activity and sustainability of laccase for degradation of the pollutant. Temperatures more than 50°C reduced the enzyme activity to about 60%. However, pH and the mediator concentration could not affect the enzyme activity. The degradation kinetic was in accordance with a Michaelis-Menten equation with Vmax and Km obtained as 0.25-0.38 μmoles/min and 0.13-0.017 mM, respectively. The stability of the immobilized enzyme was maintained for more than 85% of its initial activity after 30 days. Based on the results, it can be concluded that high resistibility and reusability of immobilized laccase on CPC-silica beads make it considerable choice for wastewater treatment.
authors.affiliation: Department of Environmental Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. a.jonidi@modares.ac.ir.
authors.forename: Emad
authors.initials: E
authors.lastname: Dehghanifard
authors.forename: Ahmad
authors.initials: A
authors.lastname: Jonidi Jafari
authors.forename: Roshanak
authors.initials: R
authors.lastname: Rezaei Kalantary
authors.forename: Amir Hosein
authors.initials: AH
authors.lastname: Mahvi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Faramarzi
authors.forename: Ali
authors.initials: A
authors.lastname: Esrafili
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