title: Charged gold nanoparticles with essentially zero serum protein adsorption in undiluted fetal bovine serum.
abstract: The adsorption of even a single serum protein molecule on a gold nanosphere used in biomedical imaging may increase the size too much for renal clearance. In this work, we designed charged ~5 nm Au nanospheres coated with binary mixed-charge ligand monolayers that do not change in size upon incubation in pure fetal bovine serum (FBS). This lack of protein adsorption was unexpected in view of the fact that the Au surface was moderately charged. The mixed-charge monolayers were composed of anionic citrate ligands modified by place exchange with naturally occurring amino acids: either cationic lysine or zwitterionic cysteine ligands. The zwitterionic tips of either the lysine or cysteine ligands interact weakly with the proteins and furthermore increase the distance between the "buried" charges closer to the Au surface and the interacting sites on the protein surface. The ~5 nm nanospheres were assembled into ~20 nm diameter nanoclusters with strong near-IR absorbance (of interest in biomedical imaging and therapy) with a biodegradable polymer, PLA(1k)-b-PEG(10k)-b-PLA(1k). Upon biodegradation of the polymer in acidic solution, the nanoclusters dissociated into primary ~5 nm Au nanospheres, which also did not adsorb any detectable serum protein in undiluted FBS.
authors.affiliation: McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712, USA.
authors.forename: Avinash K
authors.initials: AK
authors.lastname: Murthy
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Stover
authors.forename: William G
authors.initials: WG
authors.lastname: Hardin
authors.forename: Robert
authors.initials: R
authors.lastname: Schramm
authors.forename: Golay D
authors.initials: GD
authors.lastname: Nie
authors.forename: Sai
authors.initials: S
authors.lastname: Gourisankar
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Truskett
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Sokolov
authors.forename: Keith P
authors.initials: KP
authors.lastname: Johnston
mesh_terms: D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D044967:Serum; D019265:Spectroscopy, Near-Infrared
chemical_list: D001798:Blood Proteins; D006046:Gold
references.citation: ACS Nano. 2009 Sep 22;3(9):2686-96
references.pmid: 19711944
references.citation: ACS Nano. 2010 Apr 27;4(4):2178-84
references.pmid: 20373747
references.citation: Nat Mater. 2004 May;3(5):330-6
references.pmid: 15098025
references.citation: Nat Mater. 2008 Jul;7(7):588-95
references.pmid: 18500347
references.citation: J Am Chem Soc. 2005 Sep 21;127(37):12873-81
references.pmid: 16159281
references.citation: J Am Chem Soc. 2007 Nov 28;129(47):14530-1
references.pmid: 17983223
references.citation: Langmuir. 2011 May 3;27(9):5242-51
references.pmid: 21476529
references.citation: Mol Cell Proteomics. 2003 Oct;2(10):1096-103
references.pmid: 12917320
references.citation: Angew Chem Int Ed Engl. 2008;47(12):2208-12
references.pmid: 18236484
references.citation: Nat Nanotechnol. 2011 Aug 21;6(9):580-7
references.pmid: 21857686
references.citation: Langmuir. 2007 May 22;23(11):6053-62
references.pmid: 17465581
references.citation: Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14265-70
references.pmid: 18809927
references.citation: Eur Biophys J. 2011 Feb;40(2):103-15
references.pmid: 21153635
references.citation: J Phys Chem B. 2005 Feb 24;109(7):2934-41
references.pmid: 16851306
references.citation: Angew Chem Int Ed Engl. 2011 Mar 28;50(14):3168-72
references.pmid: 21374769
references.citation: ACS Nano. 2013 Jan 22;7(1):239-51
references.pmid: 23230905
references.citation: Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8
references.pmid: 6167991
references.citation: Langmuir. 2009 Mar 3;25(5):3196-9
references.pmid: 19437722
references.citation: ACS Nano. 2012 Oct 23;6(10):9182-90
references.pmid: 23009596
references.citation: Chem Rev. 2011 Sep 14;111(9):5610-37
references.pmid: 21688848
references.citation: Langmuir. 2009 Oct 6;25(19):11911-6
references.pmid: 19583183
references.citation: Langmuir. 2012 Jun 19;28(24):9131-9
references.pmid: 22515552
references.citation: Langmuir. 2010 Nov 16;26(22):16884-9
references.pmid: 20942453
references.citation: Langmuir. 2006 Sep 12;22(19):8186-91
references.pmid: 16952260
references.citation: J Am Chem Soc. 2005 Oct 19;127(41):14473-8
references.pmid: 16218643
references.citation: Langmuir. 2005 Sep 27;21(20):9303-7
references.pmid: 16171365
references.citation: Opt Lett. 2010 Nov 15;35(22):3751-3
references.pmid: 21081985
references.citation: J Am Chem Soc. 2012 Feb 1;134(4):2139-47
references.pmid: 22191645
references.citation: Langmuir. 2012 Feb 14;28(6):3159-66
references.pmid: 22216744
references.citation: Biomacromolecules. 2008 May;9(5):1357-61
references.pmid: 18376858
references.citation: J Am Chem Soc. 2011 Jul 27;133(29):11014-7
references.pmid: 21714577
references.citation: Langmuir. 2001 May 1;17(9):2841-2850
references.pmid: 34139796
references.citation: ACS Nano. 2010 Jul 27;4(7):3623-32
references.pmid: 20553005
references.citation: Biomaterials. 2009 Oct;30(29):5617-21
references.pmid: 19595457
references.citation: ACS Nano. 2010 Jan 26;4(1):365-79
references.pmid: 20020753
references.citation: Nat Biotechnol. 2007 Oct;25(10):1165-70
references.pmid: 17891134
references.citation: Biomaterials. 2009 Oct;30(29):5892-6
references.pmid: 19631374
references.citation: Nature. 2007 Feb 15;445(7129):749-53
references.pmid: 17301789
references.citation: Nanomedicine. 2009 Jun;5(2):106-17
references.pmid: 19071065
references.citation: Langmuir. 2010 Jun 1;26(11):8988-99
references.pmid: 20361735
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2050-5
references.pmid: 17267609
references.citation: Chem Soc Rev. 2012 Nov 7;41(21):6874-87
references.pmid: 22868949