title: Cadmium, mercury, and lead in kidney cortex are not associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in living kidney donors.
abstract: OBJECTIVE
  Cadmium in urine is positively associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentrations, a sensitive marker of oxidative DNA damage. We determined whether kidney concentrations of cadmium, mercury, and lead, which may generate oxidative DNA damage, were associated with urinary 8-oxodG or not.
  
  
  METHODS
  8-OxodG was measured in separate 24 h and overnight urine samples from Swedish healthy adult kidney donors (N = 152) using LC-MS/MS. Concentrations of metals were measured in kidney biopsies (N = 109) by ICP-MS.
  
  
  RESULTS
  The median 8-oxodG concentrations (adjusted to specific gravity) in 24 h and overnight samples were 13.5 and 15.3 nmol/L; 8-oxodG excretion rates in 24 h and overnight samples were 0.93 and 0.86 nmol/h. In multivariable linear regression analyses, we did not find any association between 8-oxodG concentrations or rates and elements in the kidney. The 24-h 8-oxodG concentrations were positively associated with serum ferritin (β = 0.048, p < 0.0001), body weight (β = 0.13, p = 0.0019), and inversely with gender (β = -3.34, p = 0.0024). Similar associations with 8-oxodG excretion rates were stronger. Smoking was positively associated with 24-h 8-oxodG excretion rates (β = 0.26, p = 0.0090), but not with overnight samples.
  
  
  CONCLUSIONS
  Neither cadmium, nor mercury or lead in the kidney contributed to urinary 8-oxodG concentrations in non-occupationally exposed subjects. The iron status was positively associated with urinary 8-oxodG, particularly in women.
authors.affiliation: Division of Occupational and Environmental Medicine, Department of Laboratory Medicine, Lund University, SE 22185, Lund, Sweden, Bakhtiar.Hossain@med.lu.se.
authors.forename: Mohammad Bakhtiar
authors.initials: MB
authors.lastname: Hossain
authors.forename: Lars
authors.initials: L
authors.lastname: Barregard
authors.forename: Gerd
authors.initials: G
authors.lastname: Sallsten
authors.forename: Karin
authors.initials: K
authors.lastname: Broberg
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D002104:Cadmium / Q000032:analysis*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D007501:Iron / Q000032:analysis; D007668:Kidney / Q000737:chemistry*; D016030:Kidney Transplantation / Q000706:statistics & numerical data*; D007854:Lead / Q000032:analysis*; D019520:Living Donors / Q000706:statistics & numerical data*; D008297:Male; D008628:Mercury / Q000032:analysis*; D008875:Middle Aged; D018384:Oxidative Stress; D012737:Sex Factors; D012907:Smoking / Q000453:epidemiology; D013548:Sweden
chemical_list: D002104:Cadmium; D007854:Lead; D000080242:8-Hydroxy-2'-Deoxyguanosine; D007501:Iron; D008628:Mercury; D003849:Deoxyguanosine
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