title: Gold nanoparticle as a marker for precise localization of nano-objects within intracellular sub-domains.
abstract: Delivery of nano-objects to certain intracellular sub-domains is crucial for nanomedicine. Therefore delivery of nano-object to desirable cellular compartment has to be confirmed. The most valuable confirmation of the delivery comes from direct visualization of the nano-object. This visualization usually requires use of microscope and corresponding probe which has to be conjugated with the nano-object. There are two most popular methods of the visualization: confocal and electron microscopy. The former has significant limitations due to diffraction limited resolution of confocal systems and three-dimensional convolution of fluorescence. The latter should be significantly modified for needs of the visualization. Here we describe the method for precise localization of nano-object within the cell using electron microscopy and 1-2 nm gold particles as a nanomarker.
authors.affiliation: Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
authors.forename: Valeriy
authors.initials: V
authors.lastname: Lukyanenko
authors.forename: Vadim
authors.initials: V
authors.lastname: Salnikov
mesh_terms: D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D013347:Subcellular Fractions / Q000378:metabolism*
chemical_list: D006046:Gold