title: Alteration in abundance and compartmentalization of inflammation-related miRNAs in plasma after intracerebral hemorrhage.
abstract: OBJECTIVE
  We tested the hypothesis that circulating microRNAs (miRNAs) present in plasma might display a specific signature in patients with intracerebral hemorrhage.
  
  
  METHODS
  Global miRNA profiles were determined with the Agilent Human miRNA Microarray platform, and validated by quantitative polymerase chain reaction.
  
  
  RESULTS
  A subset of 30 miRNAs were selectively upregulated in both male and female patients with intracerebral hemorrhage. Network analysis revealed that the most significantly overrepresented biological process associated with these miRNAs was inflammation. Unlike the plasma miRNAs in healthy controls, which were detected exclusively in the microvesicle fraction, the deregulated plasma miRNAs after intracerebral hemorrhage were present in both the microvesicle and the supernatant fractions.
  
  
  CONCLUSIONS
  Intracerebral hemorrhage altered both the abundance and the compartmentalization of several inflammation-related miRNAs in plasma.
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, 107 Wenhuaxi Rd, Jinan 250012, Shandong Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Guo
authors.forename: Junni
authors.initials: J
authors.lastname: Liu
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Hao
authors.forename: Xuedong
authors.initials: X
authors.lastname: Sun
authors.forename: Xiao
authors.initials: X
authors.lastname: Wu
authors.forename: Peili
authors.initials: P
authors.lastname: Bu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Fengqing
authors.initials: F
authors.lastname: Liu
authors.forename: Qunye
authors.initials: Q
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
mesh_terms: D016022:Case-Control Studies; D002543:Cerebral Hemorrhage / Q000097:blood*; D004660:Encephalitis / Q000097:blood*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D046228:Microarray Analysis; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C533097:MIR150, human; C531388:MIRN27 microRNA, human; C546819:MIRN365 microRNA, human
keywords: intracerebral hemorrhage; microRNA; microvesicle; plasma