title: Intracellular surface-enhanced Raman scattering (SERS) with thermally stable gold nanoflowers grown from Pt and Pd seeds.
abstract: SERS provides great sensitivity at low concentrations of analytes. SERS combined with near infrared (NIR)-resonant gold nanomaterials are important candidates for theranostic agents due to their combined extinction properties and sensing abilities stemming from the deep penetration of laser light in the NIR region. Here, highly branched gold nanoflowers (GNFs) grown from Pd and Pt seeds are prepared and their SERS properties are studied. The growth was performed at 80 °C without stirring, and this high temperature growth method is assumed to provide great shape stability of sharp tips in GNFs. We found that seed size must be large enough (>30 nm in diameter) to induce the growth of those SERS-active and thermally stable GNFs. We also found that the addition of silver nitrate (AgNO3) is important to induce sharp tip growth and shape stability. Incubation with Hela cells indicates that GNFs are taken up and reside in the cytoplasm. SERS was observed in those cells incubated with 1,10-phenanthroline (Phen)-loaded GNFs.
authors.affiliation: Physical Sciences and Engineering Division, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Hyon Min
authors.initials: HM
authors.lastname: Song
authors.forename: Lin
authors.initials: L
authors.lastname: Deng
authors.forename: Niveen M
authors.initials: NM
authors.lastname: Khashab
mesh_terms: D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006046:Gold / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010165:Palladium / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D010618:Phenanthrolines / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D010984:Platinum / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D012835:Silver Nitrate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D013059:Spectrum Analysis, Raman
chemical_list: D002800:Cholinesterase Inhibitors; D004337:Drug Carriers; D010618:Phenanthrolines; D010984:Platinum; D010165:Palladium; D006046:Gold; D012835:Silver Nitrate; C025205:1,10-phenanthroline