title: Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. Metabolomics and 13C-Labeling Study.
abstract: In this work we use metabolomics and (13)C-labeling data to refine central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotrophic bacterium. We demonstrate here that similar to non-methane utilizing methylotrophic alphaproteobacteria the core metabolism of the microbe is represented by several tightly connected metabolic cycles, such as the serine pathway, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle. Both in silico estimations and stable isotope labeling experiments combined with single cell (NanoSIMS) and bulk biomass analyses indicate that a significantly larger portion of the cell carbon (over 60%) is derived from CO2 in this methanotroph. Our(13) C-labeling studies revealed an unusual topology of the assimilatory network in which phosph(enol) pyruvate/pyruvate interconversions are key metabolic switches. A set of additional pathways for carbon fixation are identified and discussed.
authors.affiliation: Department of Chemical Engineering, University of Washington Seattle, WA, USA.
authors.forename: Song
authors.initials: S
authors.lastname: Yang
authors.forename: Janet B
authors.initials: JB
authors.lastname: Matsen
authors.forename: Michael
authors.initials: M
authors.lastname: Konopka
authors.forename: Abigail
authors.initials: A
authors.lastname: Green-Saxena
authors.forename: Justin
authors.initials: J
authors.lastname: Clubb
authors.forename: Martin
authors.initials: M
authors.lastname: Sadilek
authors.forename: Victoria J
authors.initials: VJ
authors.lastname: Orphan
authors.forename: David
authors.initials: D
authors.lastname: Beck
authors.forename: Marina G
authors.initials: MG
authors.lastname: Kalyuzhnaya
keywords: ethylmalonyl-CoA pathway in methanotrophs; metabolic fluxes labeling; methanotrophic proteobacteria; serine cycle
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