title: Transcriptomic analysis of Streptomyces coelicolor differentiation in solid sporulating cultures: first compartmentalized and second multinucleated mycelia have different and distinctive transcriptomes.
abstract: Streptomycetes are very important industrial bacteria, which produce two thirds of all clinically relevant secondary metabolites. They have a complex developmental-cycle in which an early compartmentalized mycelium (MI) differentiates to a multinucleated mycelium (MII) that grows inside the culture medium (substrate mycelium) until it starts to growth into the air (aerial mycelium) and ends up forming spores. Streptomyces developmental studies have focused mainly on the later stages of MII differentiation (aerial mycelium and sporulation), with regulation of pre-sporulation stages (MI/MII transition) essentially unknown. This work represents the first study of the Streptomyces MI transcriptome, analyzing how it differs from the MII transcriptome. We have used a very conservative experimental approach to fractionate MI from MII and quantify gene expressions. The expression of well characterized key developmental/metabolic genes involved in bioactive compound production (actinorhodin, undecylprodigiosin, calcium-dependent antibiotic, cpk, geosmin) or hydrophobic cover formation-sporulation (bld, whi, wbl, rdl, chp, ram) was correlated with MII differentiation. Additionally, 122 genes conserved in the Streptomyces genus, whose biological function had not been previously characterized, were found to be differentially expressed (more than 4-fold) in MI or MII. These genes encoded for putative regulatory proteins (transcriptional regulators, kinases), as well as hypothetical proteins. Knowledge about differences between the MI (vegetative) and MII (reproductive) transcriptomes represents a huge advance in Streptomyces biology that will make future experiments possible aimed at characterizing the biochemical pathways controlling pre-sporulation developmental stages and activation of secondary metabolism in Streptomyces.
authors.affiliation: Área de Microbiología, Departamento de Biología Funcional and Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, Oviedo, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Yagüe
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez-García
authors.forename: María T
authors.initials: MT
authors.lastname: López-García
authors.forename: Juan F
authors.initials: JF
authors.lastname: Martín
authors.forename: Beatriz
authors.initials: B
authors.lastname: Rioseras
authors.forename: Jesús
authors.initials: J
authors.lastname: Sánchez
authors.forename: Angel
authors.initials: A
authors.lastname: Manteca
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D012329:RNA, Bacterial
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