title: HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy.
abstract: In human transcriptional regulation, DNA-sequence-specific factors can associate with intermediaries that orchestrate interactions with a diverse set of chromatin-modifying enzymes. One such intermediary is HCFC1 (also known as HCF-1). HCFC1, first identified in herpes simplex virus transcription, has a poorly defined role in cellular transcriptional regulation. We show here that, in HeLa cells, HCFC1 is observed bound to 5400 generally active CpG-island promoters. Examination of the DNA sequences underlying the HCFC1-binding sites revealed three sequence motifs associated with the binding of (1) ZNF143 and THAP11 (also known as Ronin), (2) GABP, and (3) YY1 sequence-specific transcription factors. Subsequent analysis revealed colocalization of HCFC1 with these four transcription factors at ∼90% of the 5400 HCFC1-bound promoters. These studies suggest that a relatively small number of transcription factors play a major role in HeLa-cell transcriptional regulation in association with HCFC1.
authors.affiliation: Center for Integrative Genomics, University of Lausanne, Génopode, 1015 Lausanne, Switzerland.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Michaud
authors.forename: Viviane
authors.initials: V
authors.lastname: Praz
authors.forename: Nicole
authors.initials: N
authors.lastname: James Faresse
authors.forename: Courtney K
authors.initials: CK
authors.lastname: Jnbaptiste
authors.forename: Shweta
authors.initials: S
authors.lastname: Tyagi
authors.forename: Frédéric
authors.initials: F
authors.lastname: Schütz
authors.forename: Winship
authors.initials: W
authors.lastname: Herr
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D018899:CpG Islands; D051268:GA-Binding Protein Transcription Factor / Q000378:metabolism*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D051863:Host Cell Factor C1 / Q000378:metabolism*; D006801:Humans; D059372:Nucleotide Motifs; D056510:Position-Specific Scoring Matrices; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism*; D024363:Transcription Initiation Site; D015533:Transcriptional Activation; D050986:YY1 Transcription Factor / Q000378:metabolism*
chemical_list: D051268:GA-Binding Protein Transcription Factor; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D012333:RNA, Messenger; D012097:Repressor Proteins; C498470:THAP11 protein, human; D015534:Trans-Activators; D050986:YY1 Transcription Factor; C114253:ZNF143 protein, human
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