title: Intracellular delivery of biologically active proteins with peptide-based carriers.
abstract: The medical applications of protein-based therapeutics are hampered by low bioavailability associated with inefficient intracellular delivery. Various delivery materials have been developed and tested to interact with protein cargos in a manner of stabilizing proteins extracellularly and facilitating cellular uptake of proteins, thus enhancing delivery efficiency. Peptides that can form stable complexes with proteins through non-covalent interaction appear to be a promising tool to improve intracellular delivery of proteins. Here we describe the preparation of complexes formed between β-galactosidase and peptide-based carrier, protein transfer of the complexes, and the methods to evaluate delivery efficiency qualitatively and quantitatively.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
authors.forename: Seong Loong
authors.initials: SL
authors.lastname: Lo
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D004337:Drug Carriers; D010455:Peptides / Q000008:administration & dosage*; D011506:Proteins / Q000008:administration & dosage*
chemical_list: D004337:Drug Carriers; D010455:Peptides; D011506:Proteins