title: Human cytomegalovirus induces a biphasic inflammatory response in primary endothelial cells.
abstract: Contradictory studies report either pro- or anti-inflammatory endothelial cell (EC) responses to human cytomegalovirus (hCMV) infection, hindering the validation of a potential link between this virus and associated inflammatory pathologies. Clarifying this issue, we report that hCMV induces a biphasic response. Early after inoculation, hCMV promoted lymphocyte and, to a lesser extent, neutrophil capture under in vivo relevant shear stresses. In contrast, later stages of infection rendered EC refractory to basal, or cytokine-induced, leukocyte recruitment.
authors.affiliation: Department of Medicine, Centre for Molecular Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: H C
authors.initials: HC
authors.lastname: Jeffery
authors.forename: C
authors.initials: C
authors.lastname: Söderberg-Naucler
authors.forename: L M
authors.initials: LM
authors.lastname: Butler
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000276:immunology* / Q000821:virology; D006801:Humans; D007962:Leukocytes / Q000276:immunology / Q000821:virology
chemical_list: D016207:Cytokines
references.citation: J Virol. 2011 Jul;85(14):7321-32
references.pmid: 21543487
references.citation: Immunology. 1997 Sep;92(1):138-45
references.pmid: 9370936
references.citation: PLoS Biol. 2009 Aug;7(8):e1000177
references.pmid: 19707265
references.citation: Blood. 1994 Jan 15;83(2):370-6
references.pmid: 8286737
references.citation: Blood. 2011 Jan 6;117(1):352-61
references.pmid: 20930069
references.citation: J Virol. 2007 Mar;81(5):2095-101
references.pmid: 16956936
references.citation: J Lab Clin Med. 1999 Dec;134(6):623-30
references.pmid: 10595791
references.citation: J Leukoc Biol. 2005 Jan;77(1):44-51
references.pmid: 15459233
references.citation: Inflamm Bowel Dis. 2010 Sep;16(9):1620-7
references.pmid: 20232408
references.citation: Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3652-6
references.pmid: 8170963
references.citation: Arterioscler Thromb Vasc Biol. 2012 Sep;32(9):2045-51
references.pmid: 22895665
references.citation: Microvasc Res. 1993 Jan;45(1):33-45
references.pmid: 8479340
references.citation: Eur J Clin Invest. 1989 Dec;19(6):542-8
references.pmid: 2559852
references.citation: J Infect Dis. 1998 Jun;177(6):1465-74
references.pmid: 9607821
references.citation: Transplantation. 1999 Dec 15;68(11):1814-8
references.pmid: 10609963
references.citation: J Virol. 1999 Jan;73(1):404-10
references.pmid: 9847345
references.citation: Am J Pathol. 2004 Feb;164(2):589-600
references.pmid: 14742264
references.citation: Transplantation. 1994 Dec 27;58(12):1379-85
references.pmid: 7528950
references.citation: Blood. 2005 Jul 15;106(2):584-92
references.pmid: 15811956
references.citation: J Virol. 2006 Dec;80(23):11539-55
references.pmid: 16987970
references.citation: Transplantation. 1999 Jan 15;67(1):137-44
references.pmid: 9921810
references.citation: Immunol Cell Biol. 1998 Jun;76(3):217-21
references.pmid: 9682965
references.citation: Blood. 1993 Aug 15;82(4):1165-74
references.pmid: 7688989
references.citation: Transplantation. 2000 Mar 27;69(6):1160-8
references.pmid: 10762222
references.citation: J Periodontol. 1993 May;64(5 Suppl):456-60
references.pmid: 8315568
references.citation: Cardiovasc Res. 1996 Oct;32(4):733-42
references.pmid: 8915191
references.citation: Nat Rev Immunol. 2007 Sep;7(9):678-89
references.pmid: 17717539
references.citation: Exp Cell Res. 2005 Oct 15;310(1):22-32
references.pmid: 16109405
references.citation: J Virol. 2006 Jun;80(11):5588-98
references.pmid: 16699040