title: Effect of acute stress on immune cell counts and the expression of tight junction proteins in the duodenal mucosa of rats.
abstract: OBJECTIVE
  Duodenal immune alterations have been reported in a subset of patients with functional dyspepsia (FD). The aim of this study was to investigate the effect of acute stress on immune cell counts and the expression of tight junction proteins in the duodenal mucosa.
  
  
  METHODS
  Twenty-one male rats were divided into the following three experimental groups: 1) the nonstressed, control group, 2) the 2-hour-stressed group, and 3) the 4-hour-stressed group. Eosinophils, mast cells and CD4(+) and CD8(+) T lymphocytes in the duodenal mucosa were counted. The protein and mRNA expressions of occludin and zonula occludens-1 (ZO-1) were examined.
  
  
  RESULTS
  Eosinophils, mast cells and CD8(+) T lymphocyte counts did not differ between the stressed and control groups. The number of CD4(+) T lymphocytes and the protein and mRNA expressions of occludin and ZO-1 were significantly lower in the 4-hour-stressed group compared with the control group. The plasma adrenocorticotrophic hormone and cortisol levels of the 4-hour-stressed group were significantly higher than those of the control group.
  
  
  CONCLUSIONS
  Acute stress reduces the number of CD4(+) T lymphocytes and the expression of tight junction proteins in the duodenal mucosa, which might be associated with the duodenal immune alterations found in a subset of FD patients.
authors.affiliation: Department of Gastroenterology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Hong Sub
authors.initials: HS
authors.lastname: Lee
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Kim
authors.forename: Young Bae
authors.initials: YB
authors.lastname: Kim
authors.forename: Kwang Jae
authors.initials: KJ
authors.lastname: Lee
keywords: Duodenum; Lymphocytes; Stress; Tight junction protein
references.citation: J Physiol. 2003 Dec 15;553(Pt 3):959-66
references.pmid: 14555722
references.citation: Endocr J. 1993 Apr;40(2):213-20
references.pmid: 7951507
references.citation: Am J Physiol. 1992 Jan;262(1 Pt 1):G137-43
references.pmid: 1733259
references.citation: Neurogastroenterol Motil. 2004 Apr;16 Suppl 1:137-42
references.pmid: 15066020
references.citation: Neuroimmunomodulation. 2009;16(5):300-17
references.pmid: 19571591
references.citation: J Psychosom Res. 1994 May;38(4):281-91
references.pmid: 8064646
references.citation: Dig Dis Sci. 2002 Jan;47(1):208-15
references.pmid: 11852879
references.citation: Digestion. 2003;67(4):209-17
references.pmid: 12966228
references.citation: Dig Dis Sci. 1987 Dec;32(12):1411-7
references.pmid: 3500837
references.citation: World J Gastroenterol. 2010 Nov 28;16(44):5523-35
references.pmid: 21105185
references.citation: Gastroenterology. 2006 Apr;130(5):1466-79
references.pmid: 16678560
references.citation: Neurogastroenterol Motil. 2009 Aug;21(8):832-e56
references.pmid: 19460107
references.citation: J Neurogastroenterol Motil. 2010 Jul;16(3):251-7
references.pmid: 20680163
references.citation: Toxicol Appl Pharmacol. 2009 Nov 15;241(1):61-70
references.pmid: 19679145
references.citation: Am J Gastroenterol. 2011 Jun;106(6):1089-98
references.pmid: 21245834
references.citation: Gastroenterology. 1999 Mar;116(3):515-20
references.pmid: 10029608
references.citation: World J Gastrointest Pathophysiol. 2011 Oct 15;2(5):72-81
references.pmid: 22013552
references.citation: Aliment Pharmacol Ther. 2009 Apr 1;29(7):765-73
references.pmid: 19183150
references.citation: Dig Dis. 2001;19(3):201-11
references.pmid: 11752838
references.citation: Clin Gastroenterol Hepatol. 2007 Oct;5(10):1175-83
references.pmid: 17686660
references.citation: Neurogastroenterol Motil. 2012 Apr;24(4):318-e163
references.pmid: 22276853
references.citation: Nature. 1997 Oct 16;389(6652):737-42
references.pmid: 9338786
references.citation: Gut. 2002 Oct;51(4):507-13
references.pmid: 12235072
references.citation: Ann N Y Acad Sci. 2009 May;1165:44-52
references.pmid: 19538286
references.citation: J Clin Gastroenterol. 2009 Jul;43(6):527-33
references.pmid: 19318982
references.citation: Am J Physiol. 1998 Nov;275(5):G1037-44
references.pmid: 9815034
references.citation: Am J Physiol. 1994 Nov;267(5 Pt 1):G794-9
references.pmid: 7977741
references.citation: Scand J Gastroenterol. 1992;27(1):53-8
references.pmid: 1736343
references.citation: J Huazhong Univ Sci Technolog Med Sci. 2011 Feb;31(1):26-32
references.pmid: 21336719
references.citation: J Immunol. 1995 May 15;154(10):5511-27
references.pmid: 7730652
references.citation: Ann N Y Acad Sci. 2009 May;1165:220-7
references.pmid: 19538310
references.citation: Am J Gastroenterol. 2004 Sep;99(9):1765-73
references.pmid: 15330916
references.citation: Gut. 2002 Jul;51 Suppl 1:i54-7
references.pmid: 12077066
references.citation: Am J Physiol Gastrointest Liver Physiol. 2000 Nov;279(5):G851-7
references.pmid: 11052980