title: Three ERF transcription factors from Chinese wild grapevine Vitis pseudoreticulata participate in different biotic and abiotic stress-responsive pathways.
abstract: Ethylene response factor (ERF) functions as an important plant-specific transcription factor in regulating biotic and abiotic stress response through interaction with various stress pathways. We previously obtained three ERF members, VpERF1, VpERF2, and VpERF3 from a highly powdery mildew (PM)-resistant Chinese wild Vitis pseudoreticulata cDNA full-length library. To explore their functions associated with plant disease resistance or biotic stress, we report here to characterize three ERF members from this library. PM-inoculation analysis on three different resistant grapevine genotypes revealed that three VpERFs displayed significant responses, but a different expression pattern. Over-expression of VpERF1, VpERF2, and VpERF3 in transgenic tobacco plants demonstrated that VpERF2 and VpERF3 enhanced resistance to both bacterial pathogen Ralstonia solanacearum and fungal pathogen Phytophtora parasitica var. nicotianae Tucker. Importantly, VpERF1-overexpressing transgenic Arabidopsis plants increased susceptibility toward these pathogens. Investigation on drought, cold, and heat treatments suggested, VpERF2 was distinctly induced, whereas VpERF3 displayed a very weak response and VpERF1 was distinctly induced by drought and heat. Concurrently, VpERF3 was significantly induced by salicylic acid (SA), methyl jasmonate (MeJA), and ET. Our results showed that the three VpERFs from Chinese wild V. pseudoreticulata play different roles in either preventing disease progression via regulating the expression of relevant defense genes, or directly involving abiotic stress responsive pathways.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People's Republic of China.
authors.forename: Ziguo
authors.initials: Z
authors.lastname: Zhu
authors.forename: Jiangli
authors.initials: J
authors.lastname: Shi
authors.forename: Weirong
authors.initials: W
authors.lastname: Xu
authors.forename: Huie
authors.initials: H
authors.lastname: Li
authors.forename: Mingyang
authors.initials: M
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Tengfei
authors.initials: T
authors.lastname: Xu
authors.forename: Yazhou
authors.initials: Y
authors.lastname: Yang
authors.forename: Jiangling
authors.initials: J
authors.lastname: Cao
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000187:drug effects / Q000502:physiology; D002681:China; D018744:DNA, Plant / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013696:Temperature; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D027843:Vitis / Q000187:drug effects / Q000235:genetics / Q000382:microbiology / Q000502:physiology*
chemical_list: D018744:DNA, Plant; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors