title: Identification of serum microRNA profiles in colon cancer.
abstract: BACKGROUND
  microRNAs (miRNAs) exist in blood in an apparently stable form. We have explored whether serum miRNAs can be used as non-invasive early biomarkers of colon cancer.
  
  
  METHODS
  Serum samples from 30 patients with colon cancer stage IV and 10 healthy controls were examined for the expression of 375 cancer-relevant miRNAs. Based on the miRNA profile in this study, 34 selected miRNAs were measured in serum from 40 patients with stage I-II colon cancer and from 10 additional controls.
  
  
  RESULTS
  Twenty miRNAs were differentially expressed in serum from stage IV patients compared with controls (P<0.01). Unsupervised clustering revealed four subgroups; one corresponding mostly to the control group and the three others to the patient groups. Of the 34 miRNAs measured in the follow-up study of stage I-II patients, 21 showed concordant expression between stage IV and stage I-II patient. Based on the profiles of these 21 miRNAs, a supervised linear regression analysis (Partial Least Squares Regression) was performed. Using this model we correctly assigned stage I-II colon cancer patients based on miRNA profiles of stage IV patients.
  
  
  CONCLUSIONS
  Serum miRNA expression profiling may be utilised in early detection of colon cancer.
authors.affiliation: Department of Oncology, St Olavs Hospital, Trondheim University Hospital, Olav Kyrresgt 17, Trondheim 7006, Norway. eva.hofsli@stolav.no
authors.forename: E
authors.initials: E
authors.lastname: Hofsli
authors.forename: W
authors.initials: W
authors.lastname: Sjursen
authors.forename: W S
authors.initials: WS
authors.lastname: Prestvik
authors.forename: J
authors.initials: J
authors.lastname: Johansen
authors.forename: M
authors.initials: M
authors.lastname: Rye
authors.forename: G
authors.initials: G
authors.lastname: Tranø
authors.forename: H H
authors.initials: HH
authors.lastname: Wasmuth
authors.forename: I
authors.initials: I
authors.lastname: Hatlevoll
authors.forename: L
authors.initials: L
authors.lastname: Thommesen
mesh_terms: D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging
chemical_list: D035683:MicroRNAs
references.citation: Eur J Cancer. 2010 Jul;46(11):2059-66
references.pmid: 20621736
references.citation: Cancer Epidemiol Biomarkers Prev. 2010 Jul;19(7):1766-74
references.pmid: 20551304
references.citation: J Genet Genomics. 2010 Jun;37(6):347-58
references.pmid: 20621017
references.citation: Cancer Prev Res (Phila). 2010 Nov;3(11):1435-42
references.pmid: 20959518
references.citation: Sarcoma. 2011;2011:732690
references.pmid: 21789031
references.citation: Nat Cell Biol. 2007 Jun;9(6):654-9
references.pmid: 17486113
references.citation: Carcinogenesis. 2012 Jun;33(6):1126-33
references.pmid: 22491715
references.citation: BMC Cancer. 2010 Apr 29;10:173
references.pmid: 20429937
references.citation: Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20370-5
references.pmid: 21059916
references.citation: Biochim Biophys Acta. 2010 Dec;1806(2):200-7
references.pmid: 20637263
references.citation: Br J Cancer. 2011 Mar 15;104(6):893-8
references.pmid: 21364594
references.citation: Genes Chromosomes Cancer. 2011 Mar;50(3):196-206
references.pmid: 21213373
references.citation: J Clin Oncol. 2009 Dec 1;27(34):5848-56
references.pmid: 19884536
references.citation: J Surg Oncol. 2013 Apr;107(5):505-10
references.pmid: 22996433
references.citation: Cancer Lett. 2013 Feb 1;329(1):84-90
references.pmid: 23111103
references.citation: PLoS One. 2012;7(10):e47786
references.pmid: 23094086
references.citation: Mol Cancer. 2011 Apr 18;10:41
references.pmid: 21501493
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6982-7
references.pmid: 20351277
references.citation: Int J Cancer. 2010 Jul 1;127(1):118-26
references.pmid: 19876917
references.citation: Int J Oncol. 2012 Jun;40(6):2097-103
references.pmid: 22426940
references.citation: Nat Rev Mol Cell Biol. 2008 Mar;9(3):219-30
references.pmid: 18270516
references.citation: Mol Cancer Res. 2003 Oct;1(12):882-91
references.pmid: 14573789
references.citation: Mol Cancer. 2006 Jul 19;5:29
references.pmid: 16854228
references.citation: Gut. 2009 Nov;58(11):1546-54
references.pmid: 19834118
references.citation: Ann Surg. 2012 Sep;256(3):544-51
references.pmid: 22868372
references.citation: Int J Clin Exp Pathol. 2011 Aug 15;4(6):575-86
references.pmid: 21904633
references.citation: Clin Cancer Res. 2012 Sep 15;18(18):4919-30
references.pmid: 22850566
references.citation: Biomark Insights. 2006;2:113-121
references.pmid: 18079988
references.citation: Clin Colon Rectal Surg. 2009 Nov;22(4):209-17
references.pmid: 21037811
references.citation: Nat Rev Clin Oncol. 2011 Jun 07;8(8):467-77
references.pmid: 21647195
references.citation: Eur J Cancer. 2011 May;47(8):1127-37
references.pmid: 21402473
references.citation: Colorectal Dis. 2009 Jun;11(5):456-61
references.pmid: 19508550
references.citation: Int J Radiat Oncol Biol Phys. 2012 Jul 15;83(4):1113-9
references.pmid: 22172905
references.citation: Expert Opin Ther Targets. 2011 Mar;15(3):265-79
references.pmid: 21208133
references.citation: Cancer Res. 2012 Jul 15;72(14):3631-41
references.pmid: 22593189
references.citation: PLoS One. 2011 Mar 08;6(3):e16956
references.pmid: 21408091
references.citation: Urol Oncol. 2007 Sep-Oct;25(5):387-92
references.pmid: 17826655
references.citation: Biochem Biophys Res Commun. 2012 Apr 20;420(4):787-92
references.pmid: 22459450
references.citation: Commun Integr Biol. 2010 Sep;3(5):447-50
references.pmid: 21057637
references.citation: J Mol Biol. 2010 Sep 24;402(3):491-509
references.pmid: 20678503
references.citation: EMBO Mol Med. 2012 Mar;4(3):143-59
references.pmid: 22351564
references.citation: PLoS One. 2011 Mar 17;6(3):e17745
references.pmid: 21445232
references.citation: Lancet Oncol. 2012 Jun;13(6):e249-58
references.pmid: 22652233
references.citation: J Cell Mol Med. 2012 Nov;16(11):2655-66
references.pmid: 22469014
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20350-5
references.pmid: 18077375
references.citation: Kidney Blood Press Res. 2012;35(3):182-91
references.pmid: 22123256
references.citation: J Dig Dis. 2010 Feb;11(1):50-4
references.pmid: 20132431
references.citation: Anticancer Res. 2012 Jun;32(6):2271-5
references.pmid: 22641662
references.citation: J Natl Cancer Inst. 2012 Apr 4;104(7):528-40
references.pmid: 22395642
references.citation: J Mol Med (Berl). 2012 Dec;90(12):1421-38
references.pmid: 22660396
references.citation: CA Cancer J Clin. 2010 Sep-Oct;60(5):277-300
references.pmid: 20610543
references.citation: Oncogene. 2011 May 12;30(19):2242-51
references.pmid: 21242960
references.citation: Gut. 2012 Oct;61(10):1447-53
references.pmid: 22052060
references.citation: Nat Rev Mol Cell Biol. 2010 Apr;11(4):252-63
references.pmid: 20216554
references.citation: J Gastroenterol Hepatol. 2010 Oct;25(10):1674-80
references.pmid: 20880178
references.citation: J Surg Oncol. 2012 Dec;106(8):947-52
references.pmid: 22648208
references.citation: Cancer Res. 2008 Aug 1;68(15):6416-24
references.pmid: 18676867
references.citation: Cell Res. 2008 Oct;18(10):997-1006
references.pmid: 18766170
references.citation: J Surg Oncol. 2013 Feb;107(2):217-20
references.pmid: 22903548
references.citation: Front Genet. 2012 Oct 19;3:180
references.pmid: 23091478
references.citation: JAMA. 2008 Jan 30;299(4):425-36
references.pmid: 18230780
references.citation: Gut. 2009 Oct;58(10):1375-81
references.pmid: 19201770
references.citation: Nature. 2012 Jul 18;487(7407):330-7
references.pmid: 22810696
references.citation: PLoS One. 2010 Oct 08;5(10):e13247
references.pmid: 20949044
references.citation: Med Oncol. 2012 Dec;29(5):3113-8
references.pmid: 22562822
references.citation: Int J Colorectal Dis. 2013 Feb;28(2):247-60
references.pmid: 22903298
references.citation: Br J Cancer. 2012 Sep 25;107(7):1169-74
references.pmid: 23011541
references.citation: BMC Cancer. 2012 Mar 08;12:83
references.pmid: 22397399
references.citation: Neoplasia. 2012 Sep;14(9):868-79
references.pmid: 23019418
references.citation: Am J Pathol. 2010 Oct;177(4):1592-9
references.pmid: 20829435
references.citation: Genes Chromosomes Cancer. 2008 Sep;47(9):794-802
references.pmid: 18521848
references.citation: Mol Cancer Res. 2011 Jul;9(7):824-33
references.pmid: 21628394
references.citation: J Cancer Res Clin Oncol. 2011 Apr;137(4):557-66
references.pmid: 20508945
references.citation: Cell Metab. 2010 Oct 6;12(4):352-361
references.pmid: 20889127
references.citation: J Zhejiang Univ Sci B. 2009 Mar;10(3):219-29
references.pmid: 19283877