title: Effects of resveratrol on changes induced by high-fat feeding on clock genes in rats.
abstract: In mammals, the main component of the circadian system is the suprachiasmatic nucleus in the hypothalamus. However, circadian clocks are also present in most peripheral tissues, such as adipose tissue. The aim of the present study was to analyse the potential effects of resveratrol on changes induced by high-fat feeding in the expression of clock genes and clock-controlled genes in the white adipose tissue from rats. For this purpose, rats were divided into three groups: a control group, fed a standard diet, and two other groups, either fed a high-fat diet supplemented with resveratrol (RSV) or no resveratrol (HF). The expression of clock genes and clock-controlled genes was analysed by RT-PCR. Protein expression and fatty acid synthase (FAS) activity were also analysed. When comparing the controls, the RSV group showed similar patterns of response to the HF group, except for reverse erythroblastosis virus α (Rev-Erbα), which was down-regulated. The expression of this gene reached the same levels as in control rats. The response pattern of protein expression for Rev-Erbα was similar to that found for gene expression. High-fat feeding up-regulated all adipogenic genes and resveratrol did not modify them. In the HF group, the activity of FAS tended to increase, while resveratrol decreased. In conclusion, resveratrol reverses the change induced by high-fat feeding in the expression of Rev-Erbα in adipose tissue, which means that clock machinery is a target for this polyphenol. This change seems to be related to reduced lipogenesis, which might be involved in the body fat-lowering effect of this molecule.
authors.affiliation: Department of Nutrition and Food Sciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria, Spain.
authors.forename: Jonatan
authors.initials: J
authors.lastname: Miranda
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Madrid
authors.forename: Noemí
authors.initials: N
authors.lastname: Arias
authors.forename: M Terasa
authors.initials: MT
authors.lastname: Macarulla
authors.forename: Marta
authors.initials: M
authors.lastname: Garaulet
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects; D056926:CLOCK Proteins / Q000378:metabolism*; D002940:Circadian Rhythm; D059305:Diet, High-Fat; D015536:Down-Regulation; D064429:Fatty Acid Synthases / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050155:Lipogenesis; D008099:Liver / Q000187:drug effects; D008297:Male; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D013493:Suprachiasmatic Nucleus / Q000187:drug effects
chemical_list: D000975:Antioxidants; C498263:Nr1d1 protein, rat; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; D013267:Stilbenes; D056926:CLOCK Proteins; D064429:Fatty Acid Synthases; D000077185:Resveratrol