title: Cholesterol domains enhance transfection.
abstract: BACKGROUND
  The formation of cholesterol domains in lipoplexes has been associated with enhanced serum stability and transfection rates both in cell culture and in vivo.
  
  
  RESULTS
  This study utilizes the ability of saturated phosphatidylcholines to promote the formation of cholesterol domains at much lower cholesterol contents than have been utilized in previous work. The results demonstrate that lipoplexes with identical cholesterol and cationic lipid contents exhibit significantly improved transfection efficiencies when a domain is present, consistent with previous work. In addition, studies assessing transfection rates in the absence of serum demonstrate that the ability of domains to enhance transfection is not dependent on interactions with serum proteins. Consistent with this hypothesis, characterization of the adsorbed proteins composing the corona of these lipoplex formulations did not reveal a correlation between transfection and the adsorption of a specific protein.
  
  
  CONCLUSIONS
  We have demonstrated that the interaction with serum proteins can promote domain formation in some formulations, and thereby result in enhanced transfection only after serum exposure.
authors.affiliation: University of Colorado Denver, Skaggs School of Pharmacy & Pharmaceutical Sciences, Aurora, CO 80045, USA.
authors.forename: Jamie L
authors.initials: JL
authors.lastname: Betker
authors.forename: Max
authors.initials: M
authors.lastname: Kullberg
authors.forename: Joe
authors.initials: J
authors.lastname: Gomez
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Anchordoquy
mesh_terms: D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D002412:Cations; D002462:Cell Membrane / Q000378:metabolism*; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D061986:MCF-7 Cells; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014162:Transfection / Q000379:methods*
chemical_list: D001798:Blood Proteins; D002412:Cations; D010713:Phosphatidylcholines; D002784:Cholesterol; D008156:Luciferases
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