title: Accumulation of anthocyanin and associated gene expression in radish sprouts exposed to light and methyl jasmonate.
abstract: Radish (Raphanus sativus) sprouts have received attention as an important dietary vegetable in Asian countries. The flavonoid pathway leading to anthocyanin biosynthesis in radishes is induced by multiple regulatory genes as well as various developmental and environmental factors. This study investigated anthocyanin accumulation and the transcript level of associated genes in radish sprouts exposed to light and methyl jasmonate (MeJA). The anthocyanin content of sprouts exposed to light and treated with MeJA was higher than that of sprouts grown under dark conditions without MeJA, and the highest anthocyanin content was observed within 6-9 days after sowing (DAS). Transcript levels of almost all genes were increased in radish sprouts grown in light conditions with 100 μM MeJA relative to sprouts grown under dark conditions with or without MeJA treatment, especially at 3 DAS. The results suggest that light and MeJA treatment applied together during radish seedling development enhance anthocyanin accumulation.
authors.affiliation: Department of Crop Science and ‡Department of Bio-Environmental Chemistry, Chungnam National University , 99 Daehak-Ro, Yuseong-Gu, Daejeon 305-764, Korea.
authors.forename: Woo Tae
authors.initials: WT
authors.lastname: Park
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Seo
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Eunsook
authors.initials: E
authors.lastname: Chung
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D000085:Acetates / Q000737:chemistry*; D000872:Anthocyanins / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000737:chemistry*; D018506:Gene Expression Regulation, Plant; D008027:Light; D054883:Oxylipins / Q000737:chemistry*; D018749:RNA, Plant / Q000235:genetics; D031224:Raphanus / Q000737:chemistry* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000254:growth & development
chemical_list: D000085:Acetates; D000872:Anthocyanins; D003517:Cyclopentanes; D054883:Oxylipins; D018749:RNA, Plant; C072239:methyl jasmonate