title: Similar pyruvate kinase modifications in glioblastoma cells by 7β-hydroxycholesterol and glutamine withdrawal.
abstract: Oxysterols possess anti-proliferative properties that may be used with much effect in the treatment of cancer. We have demonstrated previously that 7 beta-hydroxycholesterol (7b-HC) provokes both metabolic stress, as witnessed by AMPK activation, and changes in lipid raft composition in C6 glioblastoma cells. These observations suggested that glycolysis might have been changed. Here we will show that 7b-HC increases cell cycle time and that it changes the affinity of pyruvate kinase to its substrate, phosphoenol pyruvate. The latter effect is mimicked by glutamine withdrawal.
authors.affiliation: Institut des Neurosciences de Montpellier, U1051 INSERM, 80 rue Augustin Fliche, 34295 Montpellier cedex 05, France. Jan.de-Weille@INSERM.fr
authors.forename: Jan
authors.initials: J
authors.lastname: de Weille
authors.forename: Christine
authors.initials: C
authors.lastname: Fabre
authors.forename: Camille
authors.initials: C
authors.lastname: Gaven
authors.forename: Norbert
authors.initials: N
authors.lastname: Bakalara
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D004789:Enzyme Activation; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D005973:Glutamine / Q000378:metabolism*; D006019:Glycolysis; D006888:Hydroxycholesterols / Q000378:metabolism* / Q000494:pharmacology; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D013312:Stress, Physiological
chemical_list: D019927:Cyclin E; D006888:Hydroxycholesterols; D005973:Glutamine; D019938:Cyclin D1; C011724:cholest-5-en-3 beta,7 alpha-diol; D010728:Phosphoenolpyruvate; D011770:Pyruvate Kinase; D055372:AMP-Activated Protein Kinases