title: Edaravone increases regional cerebral blood flow after traumatic brain injury in mice.
abstract: Traumatic brain injury (TBI) is a major cause of preventable death and serious morbidity, with subsequent low cerebral blood flow (CBF) considered to be associated with poor prognosis. In the present study, we demonstrated the effect of the free radical scavenger edaravone on regional CBF (rCBF) after TBI. Male mice (C57/BL6) were subjected to TBI using a controlled cortical impactor device. Immediately after TBI, the animals were intravenously administered 3.0 mg/kg of edaravone or a vehicle saline solution. Two-dimensional rCBF images were acquired before and 24 h post-TBI, and were quantified in the ipsilateral and contralateral hemispheres (n = 5 animals per group). CBF in the vehicle-treated animals decreased broadly over the ipsilateral hemisphere, with the region of low rCBF spreading from the frontal cortex to the occipital lobe. The zone of lowest rCBF matched that of the contusion area. The mean rCBF at 24 h for a defined elliptical region between the bregma and lambda was 73.7 ± 5.8 %. In comparison, the reduction of rCBF in edaravone-treated animals was significantly attenuated (93.4 ± 5.7 %, p < 0.05). The edaravone-treated animals also exhibited higher rCBF in the contralateral hemisphere compared with that seen in -vehicle-treated animals. It is suggested that edaravone reduces neuronal damage by scavenging reactive oxygen species (ROS) and by maintaining intact the autoregulation of the cerebral vasculature.
authors.affiliation: Department of Anatomy, Showa University School of Medicine, Shinagawa-Ku, Tokyo, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ohtaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Dohi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Tsumuraya
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Nakano
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kiriyama
authors.forename: Dandan
authors.initials: D
authors.lastname: Song
authors.forename: Tohru
authors.initials: T
authors.lastname: Aruga
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D001921:Brain / Q000098:blood supply* / Q000187:drug effects*; D001930:Brain Injuries / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004195:Disease Models, Animal; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007839:Functional Laterality; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012039:Regional Blood Flow / Q000187:drug effects
chemical_list: D016166:Free Radical Scavengers; D000077553:Edaravone; D000983:Antipyrine