title: Molecular and phylogenetic analysis of influenza A H1N1 pandemic viruses in Cuba, May 2009 to August 2010.
abstract: The influenza A(H1N1)pdm09 virus was detected in Cuba in May 2009. The introduction of a new virus with increased transmissibility into a population makes surveillance of the pandemic strain to the molecular level necessary. The aim of the present study was the molecular and phylogenetic analysis of pandemic influenza A(H1N1)pdm09 strains that circulated in Cuba between May 2009 and August 2010. Seventy clinical samples were included in the study. Nucleotide sequences from the hemagglutinin HA1 region segment were obtained directly from clinical samples. Genetic distances were calculated using MEGA v.5.05. A phylogenetic tree was constructed using MrBayes v.3.1.2 software. Potential N-glycosylation sites were predicted using NetNGlyc server 1.0. The 48 Cuban sequences of influenza A(H1N1)pdm09 obtained were similar to the A/California/07/2009 (H1N1) vaccine strain. Most of the Cuban strains belonged to clade 7. Cuban viruses showed amino acid changes, some of them located at three antigenic sites: Ca, Sa, and Sb. Two dominant mutations were detected: P83S (100%) and S203T (85.7%). Glycosylation site analysis revealed the gain of one site at position 162 in 13 sequences. The findings in this study contribute to our understanding of the progress of the influenza A(H1N1)pdm09 virus, since this virus is at the starting point of its evolution in humans.
authors.affiliation: Tropical Medicine Institute Pedro Kourí, Autopista Novia del Mediodía Km 6½, La Lisa, Ciudad de La Habana, 17100, Cuba. alexpr@ipk.sld.cu
authors.forename: Alexander Piñón
authors.initials: AP
authors.lastname: Ramos
authors.forename: Belsy Acosta
authors.initials: BA
authors.lastname: Herrera
authors.forename: Odalys Valdés
authors.initials: OV
authors.lastname: Ramírez
authors.forename: Amely Arencibia
authors.initials: AA
authors.lastname: García
authors.forename: Mayra Muné
authors.initials: MM
authors.lastname: Jiménez
authors.forename: Clara Savón
authors.initials: CS
authors.lastname: Valdés
authors.forename: Angel Goyenechea
authors.initials: AG
authors.lastname: Fernández
authors.forename: Grehete
authors.initials: G
authors.lastname: González
authors.forename: Suset I Oropesa
authors.initials: SI
authors.lastname: Fernández
authors.forename: Guelsys González
authors.initials: GG
authors.lastname: Báez
authors.forename: Bárbara Hernández
authors.initials: BH
authors.lastname: Espinosa
mesh_terms: D001499:Bayes Theorem; D003462:Cuba / Q000453:epidemiology; D006031:Glycosylation; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000453:epidemiology*; D058873:Pandemics / Q000706:statistics & numerical data*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000276:immunology
chemical_list: D012367:RNA, Viral