title: Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction.
abstract: The fabrication of patterned microstructures within three-dimensional (3D) matrices is a challenging subject in tissue engineering and regenerative medicine. A 3D, free-moving bioprinting system was developed and hydrogels were patterned by varying the process parameters of z-axis moving velocity and ejection velocity. The patterning of hydrogel based microfibers in a 3D matrigel was achieved with dimensions of 4.5 mm length and widths from 79 to 200 μm. Hyaluronan-based hydrogels mixed with fibroblasts (L929), mouse endothelial cells (MS1), or human mesenchymal stem cells (hMSCs) were patterned using a 3D moving axis bioprinter and cell behavior was monitored in culture for up to 16 days. L929 and MS1 cells and hMSCs in patterned hydrogel revealed cell-cell interactions and a morphological dependency on cell types. HMSCs formed spheres through cell aggregation, while L929 cells increased in cellular mass without cell aggregation and MS1 dispersed into the matrix instead of aggregating. The aggregation of hMSCs was attenuated by treatment with Rho kinase (ROCK) inhibitor and cadherin antibody. This reflected the close relationship between cell aggregation and migration with RhoA and cell-cell adhesion molecules. Angiogenic-specific gene expression profiles showed that expression of CD105 decreased to 22% in the ROCK inhibitor group compared to control group. These results showed that cell-based patterns in a 3D matrix are highly dependent on both cell aggregation and migration over time.
authors.affiliation: Department of Biomedical Engineering, Biomedical Science of Brain Korea 21, Medical College, Korea University, Seoul 136-705, South Korea.
authors.forename: Soyoung
authors.initials: S
authors.lastname: Hong
authors.forename: Seung-Joon
authors.initials: SJ
authors.lastname: Song
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Hwanseok
authors.initials: H
authors.lastname: Jang
authors.forename: Jaesoon
authors.initials: J
authors.lastname: Choi
authors.forename: Kyung
authors.initials: K
authors.lastname: Sun
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Park
mesh_terms: D000818:Animals; D062028:Bioprinting / Q000379:methods*; D002449:Cell Aggregation; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D006801:Humans; D006820:Hyaluronic Acid; D020100:Hydrogels / Q000737:chemistry*; D007797:Laminin; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D011509:Proteoglycans; D023822:Tissue Engineering / Q000379:methods
chemical_list: D004338:Drug Combinations; D020100:Hydrogels; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D006820:Hyaluronic Acid; D003094:Collagen