title: Androgen inhibits abdominal fat accumulation and negatively regulates the PCK1 gene in male chickens.
abstract: Capons are male chickens whose testes have been surgically incised. Capons show a significant increase in fat accumulation compared to intact male chickens. However, while caponization leads to a significant reduction in androgen levels in roosters, little is known about the molecular mechanisms through which androgen status affects lipogenesis in avian species. Therefore, investigation of the influence of androgens on fat accumulation in the chicken will provide insights into this process. In this study, Affymetrix microarray technology was used to analyze the gene expression profiles of livers from capons and intact male chickens because the liver is the major site of lipogenesis in avian species. Through gene ontology, we found that genes involved in hepatic lipogenic biosynthesis were the most highly enriched. Interestingly, among the upregulated genes, the cytosolic form of the phosphoenolpyruvate carboxykinase (PCK1) gene showed the greatest fold change. Additionally, in conjunction with quantitative real-time PCR data, our results suggested that androgen status negatively regulated the PCK1 gene in male chickens.
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Duan
authors.forename: Fan
authors.initials: F
authors.lastname: Shao
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Shao
authors.forename: Junying
authors.initials: J
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Ling
authors.forename: Kedao
authors.initials: K
authors.lastname: Teng
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
authors.forename: Changxin
authors.initials: C
authors.lastname: Wu
mesh_terms: D050153:Abdominal Fat / Q000187:drug effects / Q000378:metabolism*; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002645:Chickens / Q000235:genetics*; D004958:Estradiol / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013739:Testosterone / Q000097:blood
chemical_list: D000728:Androgens; D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D008291:Malate Dehydrogenase; C034544:D-malate dehydrogenase (decarboxylating); D010729:Phosphoenolpyruvate Carboxykinase (GTP)
references.citation: Cell Biochem Biophys. 2007;48(2-3):89-95
references.pmid: 17709878
references.citation: Nutr Metab (Lond). 2005 Nov 21;2:33
references.pmid: 16300682
references.citation: J Endocrinol. 2001 Jul;170(1):27-38
references.pmid: 11431134
references.citation: Nat Med. 1998 Jul;4(7):844-7
references.pmid: 9662379
references.citation: PLoS One. 2011;6(6):e21342
references.pmid: 21731709
references.citation: Science. 1995 Oct 20;270(5235):467-70
references.pmid: 7569999
references.citation: J Nutr. 2009 Dec;139(12):2257-65
references.pmid: 19812223
references.citation: Endocrinology. 1982 Nov;111(5):1469-75
references.pmid: 6290183
references.citation: Science. 1997 Oct 24;278(5338):680-6
references.pmid: 9381177
references.citation: Metabolism. 1991 Jan;40(1):101-4
references.pmid: 1984562
references.citation: J Biol Chem. 1981 Oct 10;256(19):10023-7
references.pmid: 7275964
references.citation: J Lipid Res. 2001 Jan;42(1):106-13
references.pmid: 11160371
references.citation: Proc Natl Acad Sci U S A. 1974 Apr;71(4):1304-8
references.pmid: 4364533
references.citation: J Clin Endocrinol Metab. 1997 Feb;82(2):407-13
references.pmid: 9024227
references.citation: Biostatistics. 2003 Apr;4(2):249-64
references.pmid: 12925520
references.citation: Nature. 2001 Feb 15;409(6822):922-7
references.pmid: 11237012
references.citation: Diabetes. 2008 Aug;57(8):2199-210
references.pmid: 18443203
references.citation: Nat Genet. 1999 Jan;21(1 Suppl):10-4
references.pmid: 9915494
references.citation: Nat Immunol. 2005 Sep;6(9):946-53
references.pmid: 16086017
references.citation: J Endocrinol. 1999 Apr;161(1):89-97
references.pmid: 10194532
references.citation: Ann Epidemiol. 1992 Sep;2(5):675-82
references.pmid: 1342319
references.citation: J Lipid Res. 2011 Jul;52(7):1352-62
references.pmid: 21504969
references.citation: J Biol Chem. 2002 Jun 28;277(26):23301-7
references.pmid: 11964395
references.citation: Gen Comp Endocrinol. 1990 Mar;77(3):466-75
references.pmid: 2338225
references.citation: J Clin Endocrinol Metab. 1996 Oct;81(10):3469-75
references.pmid: 8855787
references.citation: Mol Ther. 2006 Feb;13(2):401-10
references.pmid: 16271515
references.citation: BMC Med Genet. 2009 Sep 02;10:83
references.pmid: 19725958
references.citation: Biochim Biophys Acta. 1972 Mar 30;264(1):177-85
references.pmid: 4401804
references.citation: J Clin Endocrinol Metab. 2000 Aug;85(8):2839-53
references.pmid: 10946892
references.citation: BMC Bioinformatics. 2005 Mar 21;6:62
references.pmid: 15780134
references.citation: J Clin Endocrinol Metab. 2004 Feb;89(2):898-903
references.pmid: 14764811
references.citation: Mol Cell Biol. 2000 Sep;20(17):6508-17
references.pmid: 10938127
references.citation: Nat Genet. 1996 Dec;14(4):457-60
references.pmid: 8944026
references.citation: Endocr Rev. 1988 May;9(2):181-99
references.pmid: 3042375
references.citation: Endocrinology. 1997 Aug;138(8):3181-6
references.pmid: 9231766
references.citation: J Clin Endocrinol Metab. 1996 Dec;81(12):4358-65
references.pmid: 8954042
references.citation: J Lipid Res. 2010 Jun;51(6):1452-63
references.pmid: 20124556
references.citation: J Immunol. 2007 Mar 1;178(5):2623-9
references.pmid: 17312100
references.citation: Endocrine. 1995 Nov;3(11):789-93
references.pmid: 21153122
references.citation: Biochem J. 1975 Aug;150(2):195-203
references.pmid: 170919
references.citation: Cell. 1996 Feb 9;84(3):431-42
references.pmid: 8608597
references.citation: Poult Sci. 2007 Aug;86(8):1754-9
references.pmid: 17626821
references.citation: J Lipid Res. 1980 Sep;21(7):789-853
references.pmid: 7003040
references.citation: J Clin Endocrinol Metab. 2000 Aug;85(8):2670-7
references.pmid: 10946864
references.citation: Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9151-4
references.pmid: 8090784
references.citation: Poult Sci. 2005 Apr;84(4):547-52
references.pmid: 15844810
references.citation: Metabolism. 1971 Jan;20(1):54-62
references.pmid: 4992541
references.citation: Clin Endocrinol (Oxf). 2010 Feb;72(2):176-88
references.pmid: 19500113
references.citation: Endocrinology. 2003 Nov;144(11):5081-8
references.pmid: 12960001
references.citation: Poult Sci. 1992 Feb;71(2):357-66
references.pmid: 1546048
references.citation: J Clin Endocrinol Metab. 2000 Mar;85(3):1026-31
references.pmid: 10720034
references.citation: Proc Natl Acad Sci U S A. 1986 Oct;83(20):7583-7
references.pmid: 3094011
references.citation: J Biol Chem. 1982 Jan 10;257(1):35-8
references.pmid: 6273436
references.citation: J Biol Chem. 1984 Dec 25;259(24):15242-51
references.pmid: 6096365
references.citation: Cancer Epidemiol Biomarkers Prev. 2002 Oct;11(10 Pt 1):1041-7
references.pmid: 12376505
references.citation: Poult Sci. 2009 Sep;88(9):1832-7
references.pmid: 19687267
references.citation: Nat Biotechnol. 1996 Dec;14(13):1675-80
references.pmid: 9634850
references.citation: Nat Biotechnol. 2006 Sep;24(9):1071-3
references.pmid: 16964210
references.citation: Fertil Steril. 2006 May;85(5):1319-40
references.pmid: 16647374
references.citation: Diabetes. 2003 Jul;52(7):1649-54
references.pmid: 12829628
references.citation: J Clin Endocrinol Metab. 1995 Jan;80(1):239-43
references.pmid: 7829619
references.citation: J Anim Sci. 1971 May;32(5):849-58
references.pmid: 5571574