title: Structure of PCNA from Drosophila melanogaster.
abstract: Proliferating cell nuclear antigen (PCNA) plays essential roles in DNA replication, DNA repair, cell-cycle regulation and chromatin metabolism. The PCNA from Drosophila melanogaster (DmPCNA) was purified and crystallized. The crystal of DmPCNA diffracted to 2.0 Å resolution and belonged to space group H3, with unit-cell parameters a = b = 151.16, c = 38.28 Å. The structure of DmPCNA was determined by molecular replacement. DmPCNA forms a symmetric homotrimer in a head-to-tail manner. An interdomain connector loop (IDCL) links the N- and C-terminal domains. Additionally, the N-terminal and C-terminal domains contact each other through hydrophobic associations. Compared with human PCNA, the IDCL of DmPCNA has conformational changes, which may explain their difference in function. This work provides a structural basis for further functional and evolutionary studies of PCNA.
authors.affiliation: Department of Biology, Qingdao University, Qingdao, Shandong 266021, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Zhubing
authors.initials: Z
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Dianlin
authors.initials: D
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004331:Drosophila melanogaster / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D018809:Proliferating Cell Nuclear Antigen
keywords: DNA replication and repair; PCNA; cell-cycle regulation; homotrimer
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