title: Method for electric parametric characterization and optimization of electroporation on a chip.
abstract: We have developed a rapid method to optimize the electric parameters of cell electroporation. In our design, a pair of ring-dot formatted electrodes was used to generate a radial distribution of electric field from the center to the periphery. Varied electric field intensity was acquired in different annulus when an electric pulse was applied. Cells were cultured on the microchips for adherent cell electroporation and in situ observation. The electroporation parameters of electric field intensity were explored and evaluated in terms of cell viability and transfection efficiency. The optimization was performed in consideration of both cell viability, which was investigated to decrease as electric field increases, and the transfection rate, which normally increases at stronger electric field. The electroporation characteristics HEK-293A and Hela cells were investigated, and the optimum parameters were obtained. Verified by a commercial electroporation system as well as self-made microchips endowed the optimization with wider meaning. At last, as applications, we acquired the optimal electroporation pulse intensity of Neuro-2A cells and a type of primary cell (human umbilical vein endothelial cell, HUVEC) by one time electroporation using the proposed method.
authors.affiliation: National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Institute of Microelectronics, Peking University, Beijing 100871, China.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Wu
authors.forename: Deyao
authors.initials: D
authors.lastname: Zhao
authors.forename: Zewen
authors.initials: Z
authors.lastname: Wei
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Zhong
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
mesh_terms: D002470:Cell Survival; D004566:Electrodes; D004583:Electrons; D018274:Electroporation / Q000295:instrumentation; D004867:Equipment Design; D057809:HEK293 Cells; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans