title: Molecular cloning and expression analysis of chymotrypsin-like serine protease from the redclaw crayfish (Cherax quadricarinatus): a possible role in the junior and adult innate immune systems.
abstract: A novel chymotrypsin-like serine protease (CLSP) was isolated from the hepatopancreas of the redclaw crayfish Cherax quadricarinatus (Cq-chy). The full-length cDNA of Cq-chy contains 951 nucleotides encodes a peptide of 270 amino acids. The mature peptide comprising 223 amino acids contains the conserved catalytic triad (H, D, and S). Similarity analysis showed that Cq-chy shares high identity with chymotrypsins from the fiddler crab; Uca pugilator. Cq-chy mRNA expression in C. quadricarinatus was shown to be: (a) tissue-related with the highest expression in the hepatotpancreas and widely distributed, (b) highly responsive in the hepatopancreas to White Spot Syndrome Virus (WSSV) challenge, and (c) differently regulated in immature and adult crayfish. In this study we successfully isolated Cq-chy. Our observations indicate that Cq-chy is differently involved in the immature and adult innate immune reactions, thus suggesting a role for CLSPs in the invertebrate innate immune system.
authors.affiliation: Scientific Observing and Experimental Station of Fishery Resources and Environment in the Changjiang River, Freshwater Fisheries Research Center, Wuxi 214081, China. fangdyan@hotmail.com
authors.forename: Di-An
authors.initials: DA
authors.lastname: Fang
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Huang
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Dong-Po
authors.initials: DP
authors.lastname: Xu
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Zhou
authors.forename: Min-Ying
authors.initials: MY
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Jin-Rong
authors.initials: JR
authors.lastname: Duan
authors.forename: Wei-Gang
authors.initials: WG
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003400:Astacoidea / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D053818:Chymases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D009928:Organ Specificity; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D011994:Recombinant Proteins; D053818:Chymases