title: [Isolation and sequence analysis on virus of HFMD with encephalitis in Yantal city].
abstract: OBJECTIVE
  To learn about the pathogen spectrum and genetic characterization of HFMD with encephalitis in Yantai city.
  
  
  METHODS
  Stool samples and cerebrospinal fluids (CSF) collected from HFMD with encephalitis cases in Yantai. Virus were isolated from stool samples and identified by fluorescence reverse transcription-polymerase chain reaction. The VPl region was amplified and sequenced from positive specimens. Genetic characterization was identified by sequence analysis.
  
  
  RESULTS
  Getting 3 virus strains from 10 stool specimens and all of them belong to EV71. The nucleotide and amino acid homogeneity with the representative isolates of C4a were 98%-99% and 98.90%-99.45% respectively.
  
  
  CONCLUSIONS
  The pathogen of HFMD with encephalitis in Yantai city were mainly EV71 wich belong to subgenogroup C4 cluster C4a.
authors.affiliation: Yantai Center for Disease Control and Prevention, Yantai 264003, China.
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Han
authors.forename: Lian-Feng
authors.initials: LF
authors.lastname: Gong
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Gao
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Lv
authors.forename: Hong
authors.initials: H
authors.lastname: Memg
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D018792:Encephalitis, Viral / Q000821:virology*; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006232:Hand, Foot and Mouth Disease / Q000150:complications / Q000821:virology*; D006801:Humans; D010802:Phylogeny
chemical_list: D036022:Capsid Proteins