title: Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway.
abstract: Apoptosis plays an important role in the pathogenesis of viral infections. In this study, we investigated the cell death processes during productive HHV-6A infection and the underlying mechanisms. Annexin V-PI staining and electron microscopy indicated that HHV-6A is a strong inducer of apoptosis. HHV-6A infection decreased mitochondrial transmembrane potential and led to morphological changes of mitochondria. The cell death was associated with activation of caspase-3 and cleavage of DNA repair enzyme poly (ADP-ribose) polymerase, which is known to be an important substrate for activated caspase-3. Caspase-9 was activated significantly in HHV-6A-infected cells, whereas caspase-8 was not activated obviously. Moreover, HHV-6A infection upregulated Bax and downregulated Bcl-2. This is the first demonstration of mitochondrion-mediated, caspase-dependent apoptosis in HHV-6A-infected cells.
authors.affiliation: Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, Jiangsu 210029, China ; Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Chi
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Dandan
authors.initials: D
authors.lastname: Guo
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Genyan
authors.initials: G
authors.lastname: Liu
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Kun
authors.initials: K
authors.lastname: Yao
keywords: apoptosis; caspase; human herpesvirus 6; mitochondrion-mediated
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