title: [The relationship between two kinds of human herpesviruses and experimental gingivitis].
abstract: OBJECTIVE
  To investigate human cytomegalovirus (HCMV) and Epstein-Barr virus-type 1 (EBV-1) in GCF and saliva during experimental gingivitis in Chinese young subjects and to evaluate the effect of the virus in the initial stage of gingival inflammation.
  
  
  METHODS
  GCF of 14 and 45 and saliva without stimulating in 11 Chinese young males with healthy gingiva were collected at baseline (day 0), day 7, 14 and 21 after stopping oral hygiene and day7 after reestablishing oral hygiene (day 28). DNA of HCMV and EBV-1 were detected by nested-polymerase chain reaction (n-PCR) at the times mentioned above.
  
  
  RESULTS
  HCMV was detected in GCF of 4 subjects at baseline, 4 subjects at day 7, 3 subjects at day 14 and 2 subjects at day 21 while the subjects were different. At day 28 HCMV could not be detected. EBV-1 was not detectable in GCF during the experimental gingivitis. HCMV was detected in saliva in 4 subjects and EBV-1 was in 3 subjects. And there is no relationship between the detection of the herpesviruses and the clinical parameters as well.
  
  
  CONCLUSIONS
  We suggest that HCMV and EBV-1 are not the important factors during the initial stage of gingival inflammation.
authors.affiliation: Department of Periodontology, School and Hospital of Stomatology, Peking University, Beijing 100081, China.
authors.forename: Yi-Bing
authors.initials: YB
authors.lastname: Zhao
authors.forename: Huan-Xin
authors.initials: HX
authors.lastname: Meng
mesh_terms: D000328:Adult; D003587:Cytomegalovirus / Q000302:isolation & purification*; D005883:Gingival Crevicular Fluid / Q000821:virology*; D005891:Gingivitis / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D055815:Young Adult