title: A calibration-based approach to real-time in vivo monitoring of pyruvate C₁ and C₂ polarization using the JCC spectral asymmetry.
abstract: A calibration-based technique for real-time measurement of pyruvate polarization by partial integral analysis of the doublet from the neighbouring J-coupled carbon is presented. In vitro calibration data relating the C2 and C1 asymmetries to the instantaneous C1 and C2 polarizations, respectively, were acquired in blood. The feasibility of using the in vitro calibration data to determine the instantaneous in vivo C1 and C2 polarizations was demonstrated in the analysis of rat kidney and pig heart spectral data. An approach for incorporating this technique into in vivo protocols is proposed.
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada; Imaging Research, Sunnybrook Research Institute, Toronto, Ontario, Canada.
authors.forename: Justin Y C
authors.initials: JY
authors.lastname: Lau
authors.forename: Albert P
authors.initials: AP
authors.lastname: Chen
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Gu
authors.forename: Charles H
authors.initials: CH
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D002138:Calibration; D002247:Carbon Isotopes; D003199:Computer Systems; D007668:Kidney / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D019289:Pyruvic Acid / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D011923:Rats, Nude; D034421:Sus scrofa; D013816:Thermodynamics; D013997:Time Factors
chemical_list: D002247:Carbon Isotopes; D019289:Pyruvic Acid
keywords: DNP; asymmetry; carbon-13; hyperpolarized; polarization measurement; pyruvate