title: Effects of long-term high dose testosterone administration on vaginal epithelium structure and estrogen receptor-α and -β expression of young women.
abstract: To date, the effects of long-term testosterone (T) administration on the human vagina are not completely understood. Thus, the aim of this study was to investigate the effects of long-term T treatment on vaginal tissue histology, estrogen receptor alpha (ERα) and beta (ERβ) expression and proliferation in female to male transsexual subjects (FtM). We compared vaginal samples from FtM subjects with those of premenopausal women (PrM) and postmenopausal women (M) not receiving any hormonal treatment for at least 2 years. Vaginal tissue samples from 16 FtM subjects treated with T (intramuscular injections of 100 mg Testoviron Depot/7-10 days for at least 1 year), undergoing sex reassignment surgery, and 16 PrM and 16 M subjects undergoing a vaginal hysterectomy for prolapse, were collected. For each sample, morphology, glycogen content, proliferation (ki-67), ERα and ERβ expression were evaluated. Vaginal samples from FtM showed a loss of normal architecture of the epithelium, intermediate and superficial layers were completely lost, and glycogen content was depleted. T administration resulted in a strong proliferation reduction when compared with both M and PrM subjects. Stromal and epithelial ERα as well as ERβ were significantly decreased in FtM when compared with PrM subjects. In conclusion, our data suggests that systemic T administration at supraphysiological dosage, determines profound changes in histomorphology and reduces ERs expression and proliferation of vaginal epithelium.
authors.affiliation: 1] Center for Protection of Sexual Health, Physiopathology of Reproduction and Gynecology, Alma Mater Studiorum University of Bologna, Bologna, Italy [2] Center for Applied Biomedical Research (CRBA), S. Orsola-Malpighi University Hospital-NHS Trust, Bologna, Italy [3] Department of Clinical Medicine, Alma Mater Studiorum University of Bologna, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Baldassarre
authors.forename: F A
authors.initials: FA
authors.lastname: Giannone
authors.forename: M P
authors.initials: MP
authors.lastname: Foschini
authors.forename: C
authors.initials: C
authors.lastname: Battaglia
authors.forename: P
authors.initials: P
authors.lastname: Busacchi
authors.forename: S
authors.initials: S
authors.lastname: Venturoli
authors.forename: M C
authors.initials: MC
authors.lastname: Meriggiola
mesh_terms: D000328:Adult; D000368:Aged; D004338:Drug Combinations; D004848:Epithelium / Q000033:anatomy & histology / Q000187:drug effects*; D047628:Estrogen Receptor alpha / Q000032:analysis*; D047629:Estrogen Receptor beta / Q000032:analysis*; D005260:Female; D006003:Glycogen / Q000032:analysis; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D008875:Middle Aged; D017698:Postmenopause; D017697:Premenopause; D057829:Gender-Affirming Procedures; D013739:Testosterone / Q000008:administration & dosage*; D014189:Transsexualism; D014621:Vagina / Q000033:anatomy & histology / Q000187:drug effects*
chemical_list: D004338:Drug Combinations; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D019394:Ki-67 Antigen; D013739:Testosterone; C036098:Testoviron-depot; D006003:Glycogen