title: Bone morphogenetic protein-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect.
abstract: OBJECTIVE
  To test the osteoregenerative potential and dosing of bone morphogenetic protein-2 (BMP-2)-impregnated biomimetic scaffolds in a rat model of a mandibular defect.
  
  
  METHODS
  Prospective study using an animal model.
  
  
  METHODS
  Varied doses of BMP-2 (0.5, 1, 0.5, 0.5 in microspheres, 5, and 15 μg) were absorbed onto a biomimetic scaffold. Scaffolds were then implanted into marginal mandibular defects in rats. Blank scaffolds and unfilled defects were used as negative controls. Two months postoperatively, bone healing was analyzed with microcomputerized tomography (microCT).
  
  
  RESULTS
  MicroCT analysis demonstrated that all doses of BMP-2 induced successful healing of marginal mandibular defects in a rat mandible. Increasing doses of BMP-2 on the scaffolds produced increased tissue healing, with 15 μg demonstrating significantly more healing than all other dosing (P < .01).
  
  
  CONCLUSIONS
  BMP-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect in the rat. Percentage healing of defect, percentage of bone within healed tissue, and total bone volume are all a function of BMP-2 dosing. There appears to be an optimal dose of 5 μg beyond which there is no increase in bone volume.
  
  
  METHODS
  NA.
authors.affiliation: Department of Head and Neck Surgery, David Geffen School of Medicine at University of California at Los Angeles, Los Angeles, California, USA.
authors.forename: Adam S
authors.initials: AS
authors.lastname: DeConde
authors.forename: Douglas
authors.initials: D
authors.lastname: Sidell
authors.forename: Min
authors.initials: M
authors.lastname: Lee
authors.forename: Olga
authors.initials: O
authors.lastname: Bezouglaia
authors.forename: Kyle
authors.initials: K
authors.lastname: Low
authors.forename: David
authors.initials: D
authors.lastname: Elashoff
authors.forename: Tristan
authors.initials: T
authors.lastname: Grogan
authors.forename: Sotirios
authors.initials: S
authors.lastname: Tetradis
authors.forename: Tara
authors.initials: T
authors.lastname: Aghaloo
authors.forename: Maie
authors.initials: M
authors.lastname: St John
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D032701:Biomimetics / Q000379:methods*; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects*; D004195:Disease Models, Animal; D008334:Mandible / Q000187:drug effects* / Q000601:surgery*; D010012:Osteogenesis / Q000187:drug effects; D011446:Prospective Studies; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds
chemical_list: D055396:Bone Morphogenetic Protein 2
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