title: Notch pathway is activated by MAPK signaling and influences papillary thyroid cancer proliferation.
abstract: Mutually exclusive genetic alterations in the RET, RAS, or BRAF genes, which result in constitutively active mitogen-activated protein kinase (MAPK) signaling, are present in about 70% of papillary thyroid carcinomas (PTCs). However, the effect of MAPK activation on other signaling pathways involved in oncogenic transformation, such as Notch, remains unclear. In this study, we tested the hypothesis that the MAPK pathway regulates Notch signaling and that Notch signaling plays a role in PTC cell proliferation. Conditional induction of MAPK signaling oncogenes RET/PTC3 or BRAF(T1799A) in normal rat thyroid cell line mediated activation of Notch signaling, upregulating Notch1 receptor and Hes1, the downstream effector of Notch pathway. Conversely, pharmacological inhibition of MAPK reduced Notch signaling in PTC cell. Thyroid tumor samples from transgenic mice expressing BRAF(T1799A) and primary human PTC samples showed high levels of Notch1 expression. Down-regulation of Notch signaling by γ-secretase inhibitor (GSI) or NOTCH1 RNA interference reduces PTC cell proliferation. Moreover, the combination of GSI with a MAPK inhibitor enhanced the growth suppression in PTC cells. This study revealed that RET/PTC and BRAF(T1799A) activate Notch signaling and promote tumor growth in thyroid follicular cell. Taken together, these data suggest that Notch signaling may be explored as an adjuvant therapy for thyroid papillary cancer.
authors.affiliation: Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, Sã Paulo, Brazil.
authors.forename: Alex Shimura
authors.initials: AS
authors.lastname: Yamashita
authors.forename: Murilo Vieira
authors.initials: MV
authors.lastname: Geraldo
authors.forename: Cesar Seigi
authors.initials: CS
authors.lastname: Fuziwara
authors.forename: Marco Aurélio Vamondes
authors.initials: MA
authors.lastname: Kulcsar
authors.forename: Celso Ubirajara Moretto
authors.initials: CU
authors.lastname: Friguglietti
authors.forename: Ricardo Borges
authors.initials: RB
authors.lastname: da Costa
authors.forename: Gilson Soares
authors.initials: GS
authors.lastname: Baia
authors.forename: Edna Teruko
authors.initials: ET
authors.lastname: Kimura
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