title: Epidemiology and acquisition of extended-spectrum beta-lactamase-producing Enterobacteriaceae in a septic orthopedic ward.
abstract: Wards cohorting infected orthopaedic patients may be particularly prone to transmitting extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E). We analyze their epidemic pattern by performing molecular typing of ESBL-E isolated from patients and healthcare workers (HCW) from our septic ward. Between March 2010 and November 2011, 186 patients were admitted. Among 565 anal swabs, ESBL-E were detected in 204 samples from 45 patients, suggesting prolonged carriage in affected patients. Among 25 cases with identical ESBL-E species and positive epidemiological links, only 9 were really attributable to our service. We also screened 41 healthcare workers (HCW) on 49 occasions during the study period. Six samples (13%) were positive. None of the ESBL-E detected in HCW were related to any of the patient isolates. Among 60 environmental samples taken at the peak of the epidemic none revealed ESBL-E. We conclude that HCW also were anal carriers of ESBL-E, however the ESBL- strains from the HCW were not the same strains isolated from patients in the septic ward. Moreover, the epidemiological attribution of ESBL by simple vicinity, timing, and species identification might grossly overestimate transmission within a given unit.
authors.affiliation: Infection Control Program, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
authors.forename: Americo
authors.initials: A
authors.lastname: Agostinho
authors.forename: Gesuele
authors.initials: G
authors.lastname: Renzi
authors.forename: Thomas
authors.initials: T
authors.lastname: Haustein
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Jourdan
authors.forename: Chantal
authors.initials: C
authors.lastname: Bonfillon
authors.forename: Mathieu
authors.initials: M
authors.lastname: Rougemont
authors.forename: Pierre
authors.initials: P
authors.lastname: Hoffmeyer
authors.forename: Stephan
authors.initials: S
authors.lastname: Harbarth
authors.forename: Ilker
authors.initials: I
authors.lastname: Uçkay
keywords: Antibiotics; ESBL; Healthcare workers; Patients; Septic orthopedics
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