title: Partial protective of chickens against Eimeria tenella challenge with recombinant EtMIC-1 antigen.
abstract: Eimeria tenella microneme protein 1 (EtMIC-1) is highly conserved with TgMIC-2, which is involved in parasite binding specifically to host cells. Little is known about the immune responses and protective efficacy against E. tenella infection with EtMIC-1 antigen. In the present study, the recombinant proteins of E. tenella mature MIC-1 and adhesive domain (von Willebrand factor type A domain, EtMIC-1-VD) were obtained, protective efficacy against E.tenella infection and the mucosal immune response, which is induced in broilers was evaluated. The antibody levels and the transcription profiles of cytokine of chickens, such as interleukin-12 (IL-12) and interferon-γ (IFN-γ), were detected after being immunized three times with the recombinant EtMIC-1 and EtMIC-1-VD by ELISA assay and quantitative real-time PCR, respectively. The results showed that both groups of chickens, after being immunized with 100 μg EtMIC-1 or EtMIC-1-VD antigen, induced about tenfold higher IgG levels compared to the nonimmune groups. The transcription profiles of IL-12 and IFN-γ of the immunized groups were significantly higher than the control groups as well. The anticoccidial index of the group immunized with 100 μg EtMIC-1 and the group immunized with 100 μg EtMIC-1-VD were 167.2 and 165.5, respectively, which are significantly higher than low-dose immunized groups and challenged control groups. Our data suggests that VD domain is the key functional structure of EtMIC-1 that could trigger a significant humoral and cellular response against E. tenella infection, and EtMIC-1 had the potential in imparting partial protection in chickens against homologous challenge.
authors.affiliation: Institute for Animal Health, Guangdong Academy Agricultural Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: N S
authors.initials: NS
authors.lastname: Qi
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wang
authors.forename: S Q
authors.initials: SQ
authors.lastname: Liao
authors.forename: C Y
authors.initials: CY
authors.lastname: Wu
authors.forename: M N
authors.initials: MN
authors.lastname: Lv
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: Z X
authors.initials: ZX
authors.lastname: Tong
authors.forename: M F
authors.initials: MF
authors.lastname: Sun
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016207:Cytokines / Q000096:biosynthesis; D016786:Eimeria tenella / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control*; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016207:Cytokines; C117852:Etmic-1 protein, Eimeria tenella; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic
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