title: Large scale purification of the SERCA inhibitor thapsigargin from Thapsia garganica L. roots using centrifugal partition chromatography.
abstract: Thapsigargin (Tg) is a selective and irreversible inhibitor of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA)-dependent pump at subnanomolecular concentrations. As such, it has become a powerful tool in the study of Ca(2+) signaling pathway. Purification of Tg from Thapsia species requires repeated chromatographic steps with normal-phase alumina or silica and reverse phase chromatography. We thus developed an innovative procedure coupling high pressure automatized extraction with centrifugal partition chromatography allowing a fast and safe large-scale isolation of highly pure Tg, in two steps from Thapsia garganica L. roots. Comparison of influence of extraction procedures, storage conditions and harvesting areas on Tg content in different Algerian specimens of Thapsia garganica L. roots has been precised by mean of HPLC quantification procedure. Highest Tg content were found in the fresh material of the sample from Setif area.
authors.affiliation: Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ollivier
authors.forename: Raphaël
authors.initials: R
authors.lastname: Grougnet
authors.forename: Xavier
authors.initials: X
authors.lastname: Cachet
authors.forename: Djamila
authors.initials: D
authors.lastname: Meriane
authors.forename: Janick
authors.initials: J
authors.lastname: Ardisson
authors.forename: Sabrina
authors.initials: S
authors.lastname: Boutefnouchet
authors.forename: Brigitte
authors.initials: B
authors.lastname: Deguin
mesh_terms: D002498:Centrifugation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000737:chemistry*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D013056:Spectrophotometry, Ultraviolet; D053629:Thapsia / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases