title: ROS1 expression in invasive ductal carcinoma of the breast related to proliferation activity.
abstract: OBJECTIVE
  ROS1 is an oncogene, expressed primarily in glioblastomas of the brain that has been hypothesized to mediate the effects of early stage tumor progression. In addition, it was reported that ROS1 expression was observed in diverse cancer tissue or cell lines and ROS1 is associated with the development of several tumors. However, ROS1 expression has not been studied in breast cancer to date. Therefore, we investigated ROS1 expression at the protein and gene level to compare expression patterns and to verify the association with prognostic factors in invasive ductal carcinoma (IDC) of the breast.
  
  
  METHODS
  Tissue samples from 203 patients were used. Forty-six cases were available for fresh tissue. We performed immunohistochemical staining and real-time polymerase chain reaction (PCR).
  
  
  RESULTS
  ROS1 expression was significantly lower in proportion to higher histologic grade, higher mitotic counts, lower estrogen receptor expression, and a higher Ki-67 proliferation index, although ROS1 expression was not significantly associated with the survival rate. The result of real-time PCR revealed similar trends, however not statistically significant.
  
  
  CONCLUSIONS
  Higher ROS1 expression may be associated with favorable prognostic factors of IDC and its expression in IDC is related to the proliferation of tumor cells.
authors.affiliation: Department of Pathology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 220-701, Korea.
authors.forename: Minseob
authors.initials: M
authors.lastname: Eom
authors.forename: Sayamaa
authors.initials: S
authors.lastname: Lkhagvadorj
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Oh
authors.forename: Airi
authors.initials: A
authors.lastname: Han
authors.forename: Kwang Hwa
authors.initials: KH
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D016019:Survival Analysis
chemical_list: D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C062333:ROS1 protein, human
keywords: Breast; ROS1; invasive ductal carcinoma; prognostic factors; proliferation rate
references.citation: Nat Med. 2012 Feb 12;18(3):378-81
references.pmid: 22327623
references.citation: Oncology. 2010;79(3-4):255-61
references.pmid: 21372600
references.citation: Breast Cancer Res. 2005;7(2):R238-47
references.pmid: 15743504
references.citation: Pathol Int. 2008 Apr;58(4):226-32
references.pmid: 18324915
references.citation: Mod Pathol. 2005 Aug;18(8):1067-78
references.pmid: 15920556
references.citation: Cancer. 2003 Mar 1;97(5):1321-31
references.pmid: 12599241
references.citation: Cancer Genet Cytogenet. 1995 Sep;83(2):148-54
references.pmid: 7553586
references.citation: Genes Chromosomes Cancer. 2003 May;37(1):58-71
references.pmid: 12661006
references.citation: Pathology. 2004 Aug;36(4):295-300
references.pmid: 15370126
references.citation: Hepatology. 2007 Jan;45(1):139-49
references.pmid: 17187413
references.citation: Histopathology. 1991 Nov;19(5):403-10
references.pmid: 1757079
references.citation: Oncogene. 1994 Mar;9(3):773-80
references.pmid: 8108119
references.citation: J Clin Pathol. 2004 Jul;57(7):675-81
references.pmid: 15220356
references.citation: Cancer Res. 2007 Dec 1;67(23):11368-76
references.pmid: 18056464
references.citation: Cell. 2007 Dec 14;131(6):1190-203
references.pmid: 18083107
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):916-21
references.pmid: 12538861
references.citation: Oncogene. 2004 Feb 5;23(5):1166-76
references.pmid: 14647414
references.citation: J Clin Oncol. 2012 Mar 10;30(8):863-70
references.pmid: 22215748
references.citation: PLoS One. 2011 Jan 06;6(1):e15640
references.pmid: 21253578
references.citation: Biochim Biophys Acta. 2009 Jan;1795(1):37-52
references.pmid: 18778756
references.citation: J Thorac Oncol. 2012 Jul;7(7):1086-90
references.pmid: 22617245
references.citation: Proc Natl Acad Sci U S A. 1987 Dec;84(24):9270-4
references.pmid: 2827175
references.citation: Br J Cancer. 1957 Sep;11(3):359-77
references.pmid: 13499785
references.citation: Proc Natl Acad Sci U S A. 1990 Jun;87(12):4799-803
references.pmid: 2352949