title: Photodegradation of cobalamins in aqueous solutions and in human blood.
abstract: Vitamin B12 (cobalamin) is required for proper red blood cell formation, neurologic function, and DNA synthesis. Cobalamins in solutions are light sensitive, but no comprehensive study has been performed to compare the photostability of different cobalamins under UVA exposure. Their indirect photodegradation due to their antioxidant properties and their photostability in vivo have also not been studied so far. The photodegradation of four cobalamins (methylcobalamin (MeCbl), adenosylcobalamin (AdCbl), hydroxocobalamin (OHCbl) and cyanocobalamin (CNCbl)) under UVA exposure in aqueous solutions (pH=7.4) have been investigated by absorption spectroscopy. The photodegradation of OHCbl in the absence and presence of the endogenous photosensitizer riboflavin was studied. Serum vitamin B12 concentrations before and after summer were measured in four patients with psoriasis. All studied cobalamins are photolabile. The biologically active forms of cobalamin, AdCbl and MeCbl, are converted to OHCbl within seconds during UVA exposure. OHCbl is the most stable cobalamin. However, reactive oxygen species increases the degradation rate of OHCbl. Our pilot study on humans demonstrates that serum vitamin B12 concentrations are not significantly affected during summertime in Norway. Further work is needed to determine vitamin B12 photostability in humans living at lower latitudes or using sunbeds.
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Montebello, N-0310 Oslo, Norway. asta.juzeniene@rr-reearch.no
authors.forename: Asta
authors.initials: A
authors.lastname: Juzeniene
authors.forename: Zivile
authors.initials: Z
authors.lastname: Nizauskaite
mesh_terms: D000293:Adolescent; D006801:Humans; D008027:Light; D010782:Photolysis; D011565:Psoriasis / Q000097:blood*; D012996:Solutions; D014805:Vitamin B 12 / Q000097:blood / Q000378:metabolism / Q000528:radiation effects*; D014867:Water / Q000737:chemistry*; D056928:X-Ray Absorption Spectroscopy; D055815:Young Adult
chemical_list: D012996:Solutions; D014867:Water; D014805:Vitamin B 12