title: Scanning transmission electron microscopic tomography of cortical bone using Z-contrast imaging.
abstract: Previously we presented (McNally et al., 2012) a model for the ultrastructure of bone showing that the mineral resides principally outside collagen fibrils in the form of 5 nm thick mineral structures hundreds of nanometers long oriented parallel to the fibrils. Here we use high-angle annular dark-field electron tomography in the scanning transmission electron microscope to confirm this model and further elucidate the composite structure. Views of a section cut parallel to the fibril axes show bundles of mineral structures extending parallel to the fibrils and encircling them. The mineral density inside the fibrils is too low to be visualized in these tomographic images. A section cut perpendicular to the fibril axes, shows quasi-circular walls composed of mineral structures, wrapping around apparently empty holes marking the sites of fibrils. These images confirm our original model that the majority of mineral in bone resides outside the collagen fibrils.
authors.affiliation: Department of Materials Science and Engineering and Canadian Centre for Electron Microscopy, McMaster University, Hamilton, Ontario L8S 4M1, Canada.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: McNally
authors.forename: Feihong
authors.initials: F
authors.lastname: Nan
authors.forename: Gianluigi A
authors.initials: GA
authors.lastname: Botton
authors.forename: Henry P
authors.initials: HP
authors.lastname: Schwarcz
mesh_terms: D001842:Bone and Bones / Q000737:chemistry* / Q000648:ultrastructure*; D003094:Collagen / Q000032:analysis / Q000648:ultrastructure; D055032:Electron Microscope Tomography / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008903:Minerals / Q000032:analysis
chemical_list: D046911:Macromolecular Substances; D008903:Minerals; D003094:Collagen