title: Corticotropin-releasing factor receptor subtype 2 in human colonic mucosa: down-regulation in ulcerative colitis.
abstract: OBJECTIVE
  To assess corticotropin-releasing factor receptor 2 (CRF2) expression in the colon of healthy subjects and patients with ulcerative colitis (UC).
  
  
  METHODS
  We examined CRF2 gene and protein expression in the distal/sigmoid colonic mucosal biopsies from healthy subjects and patients with UC (active or disease in remission), human immunodeficiency virus (HIV) and functional bowel disease (FBD) by reverse transcription-polymerase chain reaction and immunofluorescence.
  
  
  RESULTS
  Gene expression of CRF2 was demonstrated in the normal human colonic biopsies, but not in the human colorectal adenocarcinoma cell line Caco2. Receptor protein localization showed immunoreactive CRF2 receptors in the lamina propria and in the epithelial cells of the distal/sigmoid biopsy samples. Interestingly, CRF2 immunoreactivity was no longer observed in epithelial cells of patients with mild-moderately active UC and disease in remission, while receptor protein expression did not change in the lamina propria. No differences in CRF2 expression profile were observed in distal/sigmoid intestinal biopsies from HIV infection and FBD patients, showing no signs of inflammation.
  
  
  CONCLUSIONS
  The down-regulation of the CRF2 receptor in the distal/sigmoid biopsies of UC patients is indicative of change in CRF2 signalling associated with the process of inflammation.
authors.affiliation: Neurocrine Biosciences Inc., San Diego, CA 92121, USA. achatzak@med.duth.gr
authors.forename: Ekaterini
authors.initials: E
authors.lastname: Chatzaki
authors.forename: Peter A
authors.initials: PA
authors.lastname: Anton
authors.forename: Mulugeta
authors.initials: M
authors.lastname: Million
authors.forename: Maria
authors.initials: M
authors.lastname: Lambropoulou
authors.forename: Theodoros
authors.initials: T
authors.lastname: Constantinidis
authors.forename: George
authors.initials: G
authors.lastname: Kolios
authors.forename: Yvette
authors.initials: Y
authors.lastname: Taché
authors.forename: Dimitri E
authors.initials: DE
authors.lastname: Grigoriadis
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D001706:Biopsy; D018938:Caco-2 Cells; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D005765:Gastrointestinal Agents / Q000627:therapeutic use; D005819:Genetic Markers; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018019:Receptors, Corticotropin-Releasing Hormone / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012720:Severity of Illness Index
chemical_list: D000893:Anti-Inflammatory Agents; C101531:CRF receptor type 2; D005765:Gastrointestinal Agents; D005819:Genetic Markers; D018019:Receptors, Corticotropin-Releasing Hormone
keywords: Colonic mucosa; Corticotropin-releasing factor; Corticotropin-releasing factor receptor; Human immunodeficiency virus; Ulcerative colitis; Urocortin
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