title: Electric field-assisted delivery of photofrin to human breast carcinoma cells.
abstract: The influence of electroporation on the Photofrin uptake and distribution was evaluated in the breast adenocarcinoma cells (MCF-7) and normal Chinese hamster ovary cells (CHO) lacking voltage-dependent channels in vitro. Photofrin was used at a concentration of 5 and 25 μM. The uptake of Photofrin was assessed using flow cytometry and fluorescence microscopy methods. Cells viability was evaluated with crystal violet assay. Our results indicated that electropermeabilization of cells, in the presence of Photofrin, increased the uptake of the photosensitizer. Even at the lowest electric field intensity (700 V/cm) Photofrin transport was enhanced. Flow cytometry results for MCF-7 cells revealed ~1.7 times stronger fluorescence emission intensity for cells exposed to Photofrin and electric field of 700 V/cm than cells treated with Photofrin alone. Photofrin was effective only when irradiated with blue light. Our studies on combination of photodynamic reaction with electroporation suggested improved effectiveness of the treatment and showed intracellular distribution of Photofrin. This approach may be attractive for cancer treatment as enhanced cellular uptake of Photofrin in MCF-7 cells can help to reduce effective dose of the photosensitizer and exposure time in this type of cancer, diminishing side effects of the therapy.
authors.affiliation: Institute of Biomedical Engineering and Instrumentation, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50-370, Wrocław, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wezgowiec
authors.forename: Maria B
authors.initials: MB
authors.lastname: Derylo
authors.forename: Justin
authors.initials: J
authors.lastname: Teissie
authors.forename: Julie
authors.initials: J
authors.lastname: Orio
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Rols
authors.forename: Julita
authors.initials: J
authors.lastname: Kulbacka
authors.forename: Jolanta
authors.initials: J
authors.lastname: Saczko
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kotulska
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D016466:CHO Cells; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D017323:Dihematoporphyrin Ether / Q000378:metabolism*; D018274:Electroporation / Q000379:methods; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D017319:Photosensitizing Agents / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D017323:Dihematoporphyrin Ether
references.citation: Cancer Treat Rev. 2012 Aug;38(5):379-86
references.pmid: 21856080
references.citation: Biochim Biophys Acta. 2006 Mar;1758(3):423-8
references.pmid: 16483538
references.citation: Clin Cancer Res. 2005 Aug 1;11(15):5381-9
references.pmid: 16061851
references.citation: Cancer Res. 1991 May 1;51(9):2251-5
references.pmid: 1826630
references.citation: Photochem Photobiol. 2002 May;75(5):534-40
references.pmid: 12017481
references.citation: Br J Cancer. 2007 Mar 26;96(6):944-51
references.pmid: 17325708
references.citation: Ultrastruct Pathol. 1996 Mar-Apr;20(2):109-129
references.pmid: 8882357
references.citation: Lasers Surg Med. 1998;22(2):74-80
references.pmid: 9484699
references.citation: Folia Biol (Praha). 2011;57(3):112-8
references.pmid: 21888834
references.citation: J Cell Physiol. 2003 Mar;194(3):363-75
references.pmid: 12548556
references.citation: Photochem Photobiol Sci. 2011 May;10(5):647-8
references.pmid: 21487635
references.citation: Anticancer Drugs. 2004 Jul;15(6):593-7
references.pmid: 15205602
references.citation: Chang Gung Med J. 2008 May-Jun;31(3):260-7
references.pmid: 18782948
references.citation: Photochem Photobiol. 1996 Jul;64(1):182-7
references.pmid: 8787012
references.citation: Folia Biol (Praha). 2007;53(1):7-12
references.pmid: 17328837
references.citation: Br J Cancer. 1996 Feb;73(4):506-13
references.pmid: 8595166
references.citation: Lasers Med Sci. 2009 Nov;24(6):971-80
references.pmid: 19653060
references.citation: Biophys J. 1992 Nov;63(5):1320-7
references.pmid: 1282374
references.citation: J Cell Physiol. 2010 Aug;224(2):414-22
references.pmid: 20432438
references.citation: Photochem Photobiol Sci. 2010 Jul 30;9(7):1033-41
references.pmid: 20532306
references.citation: Photochem Photobiol Sci. 2007 Dec;6(12):1234-45
references.pmid: 18046478
references.citation: Lasers Surg Med. 2001;29(4):305-13
references.pmid: 11746107
references.citation: Mitochondrion. 2008 Jun;8(3):237-46
references.pmid: 18514589
references.citation: J Photochem Photobiol B. 2001 Sep 15;62(3):146-52
references.pmid: 11566278
references.citation: J Photochem Photobiol B. 2009 Jul 17;96(1):1-8
references.pmid: 19406659
references.citation: Biochem Biophys Res Commun. 1995 Mar 8;208(1):26-35
references.pmid: 7887937
references.citation: Ann Biomed Eng. 2005 Feb;33(2):223-31
references.pmid: 15771276
references.citation: Folia Biol (Praha). 2008;54(1):24-9
references.pmid: 18226362
references.citation: Eur J Surg Oncol. 2008 Feb;34(2):232-40
references.pmid: 17614247
references.citation: Cancer Res. 2000 Feb 1;60(3):525-9
references.pmid: 10676629
references.citation: Am J Physiol Cell Physiol. 2007 Sep;293(3):C1010-9
references.pmid: 17596298
references.citation: Met Based Drugs. 2008;2008:286363
references.pmid: 18810273
references.citation: Photochem Photobiol. 2000 Feb;71(2):201-10
references.pmid: 10687395
references.citation: Int J Physiol Pathophysiol Pharmacol. 2011;3(2):156-66
references.pmid: 21760973
references.citation: Cancer Lett. 1997 Feb 26;113(1-2):145-51
references.pmid: 9065814
references.citation: Gene Ther. 2013 Jan;20(1):62-8
references.pmid: 22257936
references.citation: Adv Drug Deliv Rev. 2001 Jul 2;49(1-2):71-86
references.pmid: 11377804
references.citation: Med Biol Eng Comput. 2012 Dec;50(12):1213-25
references.pmid: 23179413
references.citation: Photochem Photobiol. 1997 Jan;65(1):166-76
references.pmid: 9066298
references.citation: Ann Surg Oncol. 2004 Mar;11(3):322-7
references.pmid: 14993029
references.citation: Exp Biol Med (Maywood). 2010 Jan;235(1):98-110
references.pmid: 20404024
references.citation: Medicina (Kaunas). 2009;45(5):372-7
references.pmid: 19535883
references.citation: Recent Pat Drug Deliv Formul. 2009 Jan;3(1):1-7
references.pmid: 19149725
references.citation: Recent Pat Anticancer Drug Discov. 2007 Nov;2(3):189-202
references.pmid: 18221062
references.citation: Curr Drug Metab. 2013 Mar;14(3):309-18
references.pmid: 23116111
references.citation: Acta Physiol Scand. 2003 Apr;177(4):437-47
references.pmid: 12648161
references.citation: CA Cancer J Clin. 2011 Jul-Aug;61(4):250-81
references.pmid: 21617154
references.citation: Photochem Photobiol. 2002 Feb;75(2):140-8
references.pmid: 11883602
references.citation: Acta Biochim Pol. 2003;50(2):509-13
references.pmid: 12833175
references.citation: J Cell Physiol. 1995 Oct;165(1):177-85
references.pmid: 7559799
references.citation: Bioelectrochemistry. 2004 Apr;62(1):95-8
references.pmid: 14990330
references.citation: Curr Gene Ther. 2010 Aug;10(4):255
references.pmid: 20718698
references.citation: J Pharmacol Toxicol Methods. 2005 May-Jun;51(3):177-85
references.pmid: 15862463
references.citation: Photochem Photobiol. 2006 May-Jun;82(3):617-25
references.pmid: 16475871
references.citation: J Invest Dermatol. 1998 Sep;111(3):457-63
references.pmid: 9740241