title: Gene expression profiling of intrahepatic cholangiocarcinoma.
abstract: Intrahepatic cholangiocarcinoma (ICC) is ranked as one of the top five causes of cancer-related deaths. ICC in Thai patients is associated with infection with the liver fluke, Opisthorchis viverrini, but the molecular basis for development remains unclear. The present study employed a microarray approach to compare gene expression profiles of ICCs and normal liver tissues from the same patients residing in Northeast Thailand, a region with a high prevalence of liver fluke infection. In ICC samples, 2,821 and 1,361 genes were found to be significantly up- and down-regulated respectively (unpaired t-test, p<0.05; fold-change>2.0). For validation of the microarray results, 7 up-regulated genes (FXYD3, GPRC5A, CEACAM5, MUC13, EPCAM, TMC5, and EHF) and 3 down- regulated genes (CPS1, TAT, and ITIH1) were selected for confirmation using quantitative RT-PCR, resulting in 100% agreement. The metallothionine heavy metal pathway contains the highest percentage of genes with statistically significant changes in expression. This study provides exon-level expression profiles in ICC that should be fruitful in identifying novel genetic markers for classifying and possibly early diagnosis of this highly fatal type of cholangiocarcinoma.
authors.affiliation: Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Ittisak
authors.initials: I
authors.lastname: Subrungruanga
authors.forename: Charin
authors.initials: C
authors.lastname: Thawornkunob
authors.forename: Porntip
authors.initials: P
authors.lastname: Chawalitchewinkoon-Petmitrc
authors.forename: Chawalit
authors.initials: C
authors.lastname: Pairojkul
authors.forename: Sopit
authors.initials: S
authors.lastname: Wongkham
authors.forename: Songsak
authors.initials: S
authors.lastname: Petmitrb
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001650:Bile Duct Neoplasms / Q000235:genetics* / Q000469:parasitology; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma / Q000235:genetics* / Q000469:parasitology; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D053858:Metabolic Networks and Pathways; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D009889:Opisthorchiasis / Q000150:complications; D009891:Opisthorchis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013785:Thailand; D015854:Up-Regulation