title: Overexpression of ECRG4 enhances chemosensitivity to 5-fluorouracil in the human gastric cancer SGC-7901 cell line.
abstract: The aim of this study was to examine the effects of esophageal cancer-related gene 4 (ECRG4) expression levels on chemotherapeutic sensitivity of gastric cancer cells. A SGC-7901 cell system with tetracycline-inducible ECRG4 expression (SGC-7901/ECRG4) was successfully established. ECRG4 mRNA and protein expression levels were detected using quantitative reverse transcription polymerase chain reaction and Western blotting, respectively. Chemosensitivity to 5-fluorouracil (5-FU) was examined by cell proliferation assay and cell apoptosis assay. ECRG4 mRNA and protein expression levels were significantly upregulated in SGC-7901/ECRG4 cells induced with tetracycline. Compared with control cells, the growth inhibition rate of cells with ECRG4 overexpression was significantly increased when treated with 5-FU. Treatment with 5 μmol/l 5-FU resulted in 15.2 % apoptotic cells, whereas such treatment after overexpression of ECRG4 resulted in 44.5 % apoptotic cells. In conclusion, overexpression of ECRG4 enhanced the chemosensitivity of gastric cancer SGC-7901 cells to 5-FU through induction of apoptosis.
authors.affiliation: Department of Pharmacy, Affiliated Hospital of North Sichuan Medical College, No. 63, Wenhua Rd., Nanchong, 637000, China.
authors.forename: Cheng-Ping
authors.initials: CP
authors.lastname: Jiang
authors.forename: Bi-Hua
authors.initials: BH
authors.lastname: Wu
authors.forename: Bai-Qiang
authors.initials: BQ
authors.lastname: Wang
authors.forename: Mao-Yong
authors.initials: MY
authors.lastname: Fu
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Fu
authors.initials: F
authors.lastname: Liu
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation
chemical_list: D000964:Antimetabolites, Antineoplastic; C476999:ECRG4 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D005472:Fluorouracil
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