title: Isolation of liquiritigenin-4'-apiosyl-glucoside and liquiritin from the root of Glycyrrhiza uralensis by high-performance centrifugal partition chromatography.
abstract: High-performance centrifugal partition chromatography (HPCPC) combined with ultraviolet detection was employed for the separation and purification of flavonoids from Glycyrrhiza. At the detection wavelength of 276 nm, two flavonoids, liquiritigenin-4'-apiosyl-glucoside and liquiritin, were successfully separated by HPCPC with an optimized two-phase solvent system composed of ethyl acetate-ethanol-water (1:0.1:1, v/v/v). The purity of these compounds was 95.0 and 97.1%, respectively, as determined by high-performance liquid chromatography. Their structures were identified by electrospray ion source mass spectroscopy (ESI-MS(n)) in the negative ion mode.
authors.affiliation: Central Laboratory, Changchun Normal University, China, Changchun, 130032.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Duo
authors.initials: D
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Chunming
authors.initials: C
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; C083152:liquiritigenin