title: Efficacy of parenteral vaccination against porcine circovirus type 2 (PCV2) in seropositive piglets.
abstract: This study investigated if parenteral administration of a prototype adjuvanted vaccine against porcine circovirus type 2 (PCV2) could override maternally derived antibodies and induce acquired immunity in young piglets. Piglets with high levels of maternal PCV2 antibodies at 1 wk of age were randomly grouped into vaccinates and controls on the basis of body weight and inoculated with the vaccine or a control preparation twice, with an interval of 3 wk. Both groups were challenged 3 wk after the booster vaccination and euthanized 3 wk after challenge. The pigs were evaluated for clinical disease, histologic lesions in sections of gastric and left inguinal lymph nodes stained with hematoxylin and eosin, and the amount of PCV2 antigen in the lymph nodes by immunohistochemical study. The PCV2 antibody titers were monitored by competitive enzyme-linked immunosorbent assay throughout the experiment. The vaccinates showed significantly less decline (P < 0.05) in PCV2 antibody titers after the booster vaccination. Clinical disease did not develop in any of the piglets. The vaccinates and controls did not differ in either histologic lesions or amount of PCV2 antigen in the lymph nodes. This study demonstrated some evidence of priming of young piglets in the presence of maternal antibodies. Further studies are recommended to determine the optimum concentration of PCV2 antigen and a suitable adjuvant for the vaccine to achieve the full potential of the strategy of inducing acquired immunity in young piglets that have maternally derived antibodies.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4. lag153@mail.usask.ca
authors.forename: Lakshman N A
authors.initials: LN
authors.lastname: Gamage
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: McIntosh
authors.forename: Sarah
authors.initials: S
authors.lastname: Parker
authors.forename: John
authors.initials: J
authors.lastname: Harding
authors.forename: Steven
authors.initials: S
authors.lastname: Krakowka
authors.forename: John
authors.initials: J
authors.lastname: Ellis
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology; D000818:Animals; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007112:Immunity, Maternally-Acquired / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D008198:Lymph Nodes / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D014765:Viral Vaccines
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