title: Prognostic value of changes in the expression of stem cell markers in the peripheral blood of patients with colon cancer.
abstract: Cancer stem cells play an important role in carcinogenesis and resistance to treatment and may lead to metastasis. The isolation of circulating stem cells involves cell sorting based on the presence of cell surface markers. Many surface markers such as CD133, c-Kit, SOX, OCT4 and TWIST have been reported. In the present study, we determined the expression of different stem cell markers and their variation in expression at different stages of the treatment process. Samples of EDTA blood were collected from metastatic colorectal cancer patients, and circulating cancer stem cells were isolated for the analysis of the expression of stem cell markers using RT-PCR. These findings were correlated with the response to therapy. All statistical analyses were performed using the GraphPad Prism 5.03 software. Significant differences were found in the expression levels of the markers CD133, SOX2, OCT4 and TWIST1. No differences were found in c-Kit expression. Correlation in the expression levels of most of the markers was observed. Expression of CD133, OCT4, SOX2 and TWIST1 had a predictive value for colon cancer behavior. Evaluation of this stem cell gene expression panel may be useful for predicting the response during the process of treatment, and the relative easy access to samples facilitates this method. Moreover the correlation between CD133 and TWIST1 expression may be associated with tumor regrowth and metastatic relapse.
authors.affiliation: Department of Medical Oncology, University Hospital of Santiago de Compostela, La Coruña, Galicia, Spain. mariaelena.padin.iruegas@sergas.es
authors.forename: Maria-Elena
authors.initials: ME
authors.lastname: Padín-Iruegas
authors.forename: Michel
authors.initials: M
authors.lastname: Herranz-Carnero
authors.forename: Santiago
authors.initials: S
authors.lastname: Aguin-Losada
authors.forename: Elena
authors.initials: E
authors.lastname: Brozos-Vazquez
authors.forename: U
authors.initials: U
authors.lastname: Anido-Herranz
authors.forename: Jose-Ramon
authors.initials: JR
authors.lastname: Antunez-Lopez
authors.forename: Alvaro
authors.initials: A
authors.lastname: Ruibal-Morell
authors.forename: Rafael
authors.initials: R
authors.lastname: López-López
mesh_terms: D000071916:AC133 Antigen; D000230:Adenocarcinoma / Q000097:blood* / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000097:blood* / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D009687:Nuclear Proteins / Q000097:blood; D050814:Octamer Transcription Factor-3 / Q000097:blood; D010455:Peptides / Q000097:blood; D011379:Prognosis; D019009:Proto-Oncogene Proteins c-kit / Q000097:blood; D055748:SOXB1 Transcription Factors / Q000097:blood; D051879:Twist-Related Protein 1 / Q000097:blood
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D009687:Nuclear Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C000606023:PROM1 protein, human; D010455:Peptides; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; D019009:Proto-Oncogene Proteins c-kit