title: Lactobacillus plantarum LG42 isolated from gajami sik-hae inhibits adipogenesis in 3T3-L1 adipocyte.
abstract: We investigated whether lactic acid bacteria isolated from gajami sik-hae (GLAB) are capable of reducing the intracellular lipid accumulation by downregulating the expression of adipogenesis-related genes in differentiated 3T3-L1 cells. The GLAB, Lactobacillus plantarum LG42, significantly decreased the intracellular triglyceride storage and the glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner. mRNA expression of transcription factors like peroxisome proliferator-activated receptor (PPAR) γ and CCAAT/enhancer-binding protein (C/EBP) α involved in adipogenesis was markedly decreased by the GLAB treatment. Moreover, the GLAB also decreased the expression level of adipogenic markers like adipocyte fatty acid binding protein (aP2), leptin, GPDH, and fatty acid translocase (CD36) significantly. These results suggest that the GLAB inhibits lipid accumulation in the differentiated adipocyte through downregulating the expression of adipogenic transcription factors and other specific genes involved in lipid metabolism.
authors.affiliation: Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Suk-Heung
authors.initials: SH
authors.lastname: Oh
authors.forename: Youn-Soo
authors.initials: YS
authors.lastname: Cha
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D047495:PPAR gamma / Q000096:biosynthesis / Q000235:genetics; D050656:Transcription Factor AP-2 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D047495:PPAR gamma; C493668:Tfap2a protein, mouse; D050656:Transcription Factor AP-2; D005993:Glycerolphosphate Dehydrogenase
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