title: Single cell time-lapse imaging of focus formation by the DNA damage-response protein 53BP1 after UVC irradiation of human pancreatic cancer cells.
abstract: We have previously demonstrated that ultraviolet (UV) light treatment is effective against various types of cancer cells expressing fluorescent proteins. In order to further understand the efficacy of UV treatment of cancer cells, we determined the kinetics of focus formation by imaging of a DNA damage-response (DDR) protein after UVC irradiation of human pancreatic cancer cells. A fusion protein consisting of the DDR protein 53BP1 and green fluorescent protein (GFP) (GFP-53BP1) was used as a live-cell imaging marker for cellular response after UVC irradiation. GFP-53BP1 foci were observed after UVC irradiation of MiaPaCa-2 human pancreatic cancer cells. During live-cell imaging, GFP-53BP1 foci were observed in the cells within 15 min after UVC irradiation, and some of the foci remained stable for at least three hours. GFP-53BP1 focus formation was observed in the pancreatic-cancer cells irradiated by 25-200 J/m(2) UVC. Our results indicate that an early response to DNA damage caused by UVC irradiation can be visualized by increased GFP-53BP1 focus formation by pancreatic cancer cells.
authors.affiliation: AntiCancer, Inc, San Diego, CA 92111, USA.
authors.forename: Shinji
authors.initials: S
authors.lastname: Miwa
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Tome
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Hiroshima
authors.forename: Fuminari
authors.initials: F
authors.lastname: Uehara
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kimura
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Hayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.forename: Elena V
authors.initials: EV
authors.lastname: Efimova
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy; D059008:Time-Lapse Imaging; D014407:Tumor Cells, Cultured; D000071857:Tumor Suppressor p53-Binding Protein 1; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1; D049452:Green Fluorescent Proteins