title: A role for eosinophils in the intestinal immunity against infective Ascaris suum larvae.
abstract: The aim of this study was to explore the mechanisms of resistance against invading Ascaris suum larvae in pigs. Pigs received a low dose of 100 A. suum eggs daily for 14 weeks. This resulted in a >99% reduction in the number of larvae that could migrate through the host after a challenge infection of 5000 A. suum eggs, compared to naïve pigs. Histological analysis at the site of parasite entry, i.e. the caecum, identified eosinophilia, mastocytosis and goblet cell hyperplasia. Increased local transcription levels of genes for IL5, IL13, eosinophil peroxidase and eotaxin further supported the observed eosinophil influx. Further analysis showed that eosinophils degranulated in vitro in response to contact with infective Ascaris larvae in the presence of serum from both immune and naïve animals. This effect was diminished with heat-inactivated serum, indicating a complement dependent mechanism. Furthermore, eosinophils were efficient in killing the larvae in vitro when incubated together with serum from immune animals, suggesting that A. suum specific antibodies are required for efficient elimination of the larvae. Together, these results indicate an important role for eosinophils in the intestinal defense against invading A. suum larvae.
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: Dries
authors.initials: D
authors.lastname: Masure
authors.forename: Johnny
authors.initials: J
authors.lastname: Vlaminck
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Koen
authors.initials: K
authors.lastname: Chiers
authors.forename: Wim
authors.initials: W
authors.lastname: Van den Broeck
authors.forename: Jozef
authors.initials: J
authors.lastname: Vercruysse
authors.forename: Peter
authors.initials: P
authors.lastname: Geldhof
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D001196:Ascariasis / Q000276:immunology* / Q000469:parasitology; D017165:Ascaris suum / Q000276:immunology*; D002432:Cecum / Q000276:immunology* / Q000473:pathology; D003165:Complement System Proteins / Q000276:immunology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006651:Histocytochemistry; D007814:Larva / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D003165:Complement System Proteins
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