title: Function and horizontal transfer of the small terminase subunit of the tailed bacteriophage Sf6 DNA packaging nanomotor.
abstract: Bacteriophage Sf6 DNA packaging series initiate at many locations across a 2kbp region. Our in vivo studies show that Sf6 small terminase subunit (TerS) protein recognizes a specific packaging (pac) site near the center of this region, that this site lies within the portion of the Sf6 gene that encodes the DNA-binding domain of TerS protein, that this domain of the TerS protein is responsible for the imprecision in Sf6 packaging initiation, and that the DNA-binding domain of TerS must be covalently attached to the domain that interacts with the rest of the packaging motor. The TerS DNA-binding domain is self-contained in that it apparently does not interact closely with the rest of the motor and it binds to a recognition site that lies within the DNA that encodes the domain. This arrangement has allowed the horizontal exchange of terS genes among phages to be very successful.
authors.affiliation: Biology Department, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Justin C
authors.initials: JC
authors.lastname: Leavitt
authors.forename: Eddie B
authors.initials: EB
authors.lastname: Gilcrease
authors.forename: Kassandra
authors.initials: K
authors.lastname: Wilson
authors.forename: Sherwood R
authors.initials: SR
authors.lastname: Casjens
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000502:physiology*; D042003:DNA Packaging; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D021122:Protein Subunits; D014764:Viral Proteins; D004706:Endodeoxyribonucleases; C030894:terminase
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