title: Detecting low-affinity extracellular protein interactions using protein microarrays.
abstract: Low-affinity extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high-throughput manner. This unit describes a protein microarray platform that significantly improves the throughput of assays capable of detecting transient extracellular protein interactions. These methodological improvements now permit screening for novel extracellular receptor-ligand interactions on a genome-wide scale.
authors.affiliation: Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Gavin J
authors.initials: GJ
authors.lastname: Wright
mesh_terms: D005110:Extracellular Space / Q000737:chemistry* / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D011506:Proteins