title: Effect of a combination of low level ozone and metal ions on reducing Escherichia coli O157:H7 and Listeria monocytogenes.
abstract: Ozonated water has been used as a strong antimicrobial agent against foodborne pathogens. In this study, the combined effect of low level ozonated water and different added components, including 0.2% starch and metal ions (1 mM CuCl2·2H2O and 0.1 mM AgNO3), on inactivation of Escherichia coli O157:H7 and Listeria monocytogenes was investigated. Treatment with 0.4 ppm ozonated water for 30 min resulted in a maximum log reduction in E. coli O157:H7 and L. monocytogenes compared to initial bacterial counts. The log reductions of bacteria in a starch solution containing ozonated water were slightly higher than those in ozonated water alone. Furthermore, the log reductions of E. coli O157:H7 (2.59 and 4.71 log cfu/mL) and L. monocytogenes (2.53 and 4.28 log cfu/mL) in a metal ion solution containing 0.2 and 0.4 ppm ozone for 30 min were significantly higher than those of the water and starch added groups (p < 0.05). These results indicate that a combination of ozonated water and metal ions may be useful as a antimicrobial agent.
authors.affiliation: Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Gyeongnam 660-758, Korea. white@gntech.ac.kr
authors.forename: Suk-Nam
authors.initials: SN
authors.lastname: Kang
authors.forename: Kui-Jin
authors.initials: KJ
authors.lastname: Kim
authors.forename: Joung-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Ok-Hwan
authors.initials: OH
authors.lastname: Lee
mesh_terms: D019453:Escherichia coli O157 / Q000187:drug effects*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology / Q000379:methods; D007477:Ions / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects*; D008670:Metals / Q000737:chemistry / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D013213:Starch / Q000737:chemistry / Q000494:pharmacology; D014867:Water / Q000737:chemistry / Q000494:pharmacology
chemical_list: D007477:Ions; D008670:Metals; D014867:Water; D010126:Ozone; D013213:Starch
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