title: Evaluation of normal and metastatic mammary cells grown in different biomaterial matrices: establishing potential tissue test systems.
abstract: The in vitro growth and differentiation of normal mammalian cells is quite different than the growth of cells derived from tumors. Additionally, cells of the same origin (tissue) behave differently depending on the biomaterial matrix in or on which they are grown in vitro. We examined both Matrigel(TM) and a collagen/agarose blend and demonstrated that two murine mammary derived cells lines, 4T1 and NMuMG, derived from a metastatic mammary tumor or a normal mammary gland, respectively, exhibit different growth and differentiation patterns depending on the three-dimensional matrix in which they are grown. The shape and size of the colonies that formed were matrix dependent. The two cell lines produced different levels of growth factors and metalloproteinases, and expressed differentiation markers specific to a matrix. Through the classification of different cell behaviors in different growth matrices, we will be able to intelligently design and tune tissue test systems to ask and answer specific challenging scientific questions.
authors.affiliation: Institute for Biological Interfaces of Engineering, 401-2 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634, USA.
authors.forename: Brian W
authors.initials: BW
authors.lastname: Booth
authors.forename: Jang Pyo
authors.initials: JP
authors.lastname: Park
authors.forename: Karen J L
authors.initials: KJ
authors.lastname: Burg
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D001940:Breast / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D016764:Cell Polarity; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D004338:Drug Combinations; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D012685:Sepharose / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D036341:Intercellular Signaling Peptides and Proteins; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D012685:Sepharose; D020782:Matrix Metalloproteinases