title: Evaluation of Luteolin in the Prevention of N-nitrosodiethylamine-induced Hepatocellular Carcinoma Using Animal Model System.
abstract: Hepatocellular carcinoma (HCC) is one of the commonest tumors worldwide. The treatment of HCC is vital for disease diagnosis and prognosis, as the liver is the most important organ controlling metabolic functions. Now-a-days, western folklore medicines are largely dependent on the phyto compounds which are highly effective in therapy and with low side effects. Luteolin is a flavonoid (3,4,5,7-Tetrahydro flavones) possess anti-inflammatory, anticancer and anti allergic property. The present study evaluates the efficacy of luteolin against N-nitrosodiethylamine (DEN) induced HCC in albino rats. In the highlight of the above, luteolin was evaluated for its efficacy against DEN induced HCC in male Wistar albino rats. The Biochemical parameters such as tissue damaging enzymes viz., AST, ALP, LDH and γ-GT, enzymatic antioxidants viz., SOD, CAT, GSH and GPx and histopathological changes have been estimated. The tissue damaging enzymes were found to be high in DEN alone treated group whereas the enzymatic antioxidants decreased destructively. Severe lesions and cirrhosis were observed in the toxin (DEN alone) treated group. The luteolin treated DEN group altered the tissue damaging enzymes and the enzymatic antioxidants. The damaged lesion in the histoarchitecture of DEN treated rat liver was almost completely restored. Finally this study strongly demonstrates that luteolin has potent curative property against HCC in albino rats.
authors.affiliation: Department of Biochemistry, PRIST University, Thanjavur, South India.
authors.forename: K
authors.initials: K
authors.lastname: Balamurugan
authors.forename: J
authors.initials: J
authors.lastname: Karthikeyan
keywords: DEN; Enzymatic/Non-enzymatic Antioxidants; Hepatocellular carcinoma; Luteolin; Ultrastructural study
references.citation: J Ethnopharmacol. 2004 Mar;91(1):65-8
references.pmid: 15036470
references.citation: Arch Biochem Biophys. 1959 May;82(1):70-7
references.pmid: 13650640
references.citation: J Lab Clin Med. 1967 Jul;70(1):158-69
references.pmid: 6066618
references.citation: Biochem Pharmacol. 1999 Sep 1;58(5):759-65
references.pmid: 10449184
references.citation: J Ethnopharmacol. 2006 Feb 20;103(3):484-90
references.pmid: 16213120
references.citation: Int J Cancer. 1999 Mar 15;80(6):827-41
references.pmid: 10074914
references.citation: Chem Biol Interact. 2006 Jun 10;161(2):104-14
references.pmid: 16643877
references.citation: Planta Med. 2000 Feb;66(1):25-9
references.pmid: 10705729
references.citation: Mutat Res. 1989 Sep;214(1):33-40
references.pmid: 2671699
references.citation: J Electron Microsc (Tokyo). 1994 Dec;43(6):347-55
references.pmid: 7722427
references.citation: Free Radic Res. 2000 Nov;33(5):623-33
references.pmid: 11200093
references.citation: Carcinogenesis. 1984 Nov;5(11):1381-93
references.pmid: 6386215
references.citation: Biochem Pharmacol. 2004 Jun 1;67(11):2103-14
references.pmid: 15135307
references.citation: Clin Chem. 1976 Dec;22(12):2051-5
references.pmid: 11903
references.citation: Free Radic Biol Med. 1994 Sep;17(3):235-48
references.pmid: 7982629
references.citation: Free Radic Res. 2009 Feb;43(2):128-37
references.pmid: 19115120
references.citation: J Surg Res. 2005 Aug;127(2):112-7
references.pmid: 16083749
references.citation: Cancer Lett. 2000 Dec 20;161(2):221-9
references.pmid: 11090973
references.citation: Chem Biol Interact. 2005 Oct 20;156(2-3):101-11
references.pmid: 16144695
references.citation: Cancer Lett. 1999 Feb 8;136(1):11-6
references.pmid: 10211933
references.citation: J Biol Chem. 1974 Nov 25;249(22):7130-9
references.pmid: 4436300
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Am J Clin Pathol. 1957 Jul;28(1):56-63
references.pmid: 13458125