title: Transport across Caco-2 monolayers of peptides arising from in vitro digestion of bovine milk proteins.
abstract: The entire panel of peptides produced from caseins (CN) and whey proteins (WP) that survive in vitro sequential gastro-pancreatic digestion and translocate across monolayers of Caco-2 cells, used as a model of the intestinal epithelium, has been characterised by HPLC and mass spectrometry. Among the milk-derived bioactive peptides, only minor amounts of mono-phosphorylated peptides arising from αs1- and β-CN were detected. The absorption behaviour of two resistant β-lactoglobulin (β-Lg) domains, β-Lg 125-135 and β-Lg 40-60, was studied in detail using synthetic peptides. The IgE-binding properties of the digests recovered from the apical and basolateral monolayer compartments were evaluated by dot-blot, using the sera of milk allergic children (N=5). Outcomes indicated β-Lg 127-135 as a possible "immune sensitising factor"in vivo. The almost complete loss of the IgE-affinity of CN and WP after digestion points out the need to design in vivo experiments to track the metabolic fate of dietary proteins.
authors.affiliation: Istituto di Scienze dell'Alimentazione (ISA), CNR, Via Roma 64, 83100 Avellino, Italy. picariello@isa.cnr.it
authors.forename: Gianluca
authors.initials: G
authors.lastname: Picariello
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Iacomino
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Mamone
authors.forename: Pasquale
authors.initials: P
authors.lastname: Ferranti
authors.forename: Olga
authors.initials: O
authors.lastname: Fierro
authors.forename: Carmen
authors.initials: C
authors.lastname: Gianfrani
authors.forename: Aldo
authors.initials: A
authors.lastname: Di Luccia
authors.forename: Francesco
authors.initials: F
authors.lastname: Addeo
mesh_terms: D000818:Animals; D001692:Biological Transport; D018938:Caco-2 Cells; D002364:Caseins / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004063:Digestion; D006801:Humans; D007700:Kinetics; D008894:Milk Proteins / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D000067816:Whey Proteins
chemical_list: D002364:Caseins; D008894:Milk Proteins; D010455:Peptides; D000067816:Whey Proteins