title: Preventing adsorption of immunoglobulin G to solid surfaces using poloxamer 407 eliminates artifactual stimulation of neutrophils.
abstract: To study the effect of polyclonal intravenous immunoglobulin G (IVIG) on neutrophils in vitro, adsorption of immunoglobulin G (IgG) to solid surfaces has to be prevented, because IgG bound to a solid surface can activate neutrophils through activating FcγRs. In this study we demonstrate that poloxamer 407, a non ionic surfactant, at low concentration (0.05%) prevented the adsorption of high concentrations of IgG (5 mg/ml) better than other blocking agents without interfering with the interaction of IgG with the neutrophils. Poloxamer 407 is therefore a suitable blocking agent to prevent the interaction of immunoglobulin with solid surfaces in cell-based in vitro experiments.
authors.affiliation: Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands. I.Kustiawan@Sanquin.nl
authors.forename: Iwan
authors.initials: I
authors.lastname: Kustiawan
authors.forename: Ninotska I L
authors.initials: NI
authors.lastname: Derksen
authors.forename: Theo
authors.initials: T
authors.lastname: Rispens
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008823:Micelles; D009504:Neutrophils / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D008823:Micelles; D013501:Surface-Active Agents; D020442:Poloxamer