title: mRNA profiles of cytokine receptors in unstimulated peripheral blood mononuclear cells from patients with chronic idiopathic urticaria.
abstract: This present study was aimed to investigate the roles of the receptors of Th1/Th2 cytokines and chemokines in the pathogenesis of chronic idiopathic urticaria (CIU). Thirty patients with CIU, 30 patients with dermographism and 30 healthy controls were randomly enrolled. Reverse transcription-PCR (RT-PCR) was used to analyze the mRNA of cytokine receptors in peripheral blood mononuclear cells (PBMCs). The mRNA levels of tumor necrosis factor receptor (TNFR), interferon-γ receptor (IFN-γR), and interleukin-10 receptor (IL-10R) were statistically increased in the CIU group (P < 0.05), while IL-2R, IL-4R, IL-6R, and IL-13R showed no significant differences between the CIU and other groups. The mRNA levels of CCR3 and CCR6 were statistically increased in the CIU group (P < 0.05). The toll-like receptor 2 (TLR2) mRNA level was significantly lower in the CIU group than the healthy control group (P < 0.05). These findings indicate that the regulation of mRNA of TNFR, IFN-γR, IL-10R, CCR3, CCR6 and TLR2 may be involved in the pathogenesis of CIU.
authors.affiliation: Department of Dermatology, Haidian Hospital, Beijing 10080, China ; Section of Clinical Immunology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, Jiangsu, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Gao
authors.forename: Aizhen
authors.initials: A
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Ansheng
authors.initials: A
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Yao
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.forename: Shihai
authors.initials: S
authors.lastname: Xie
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Yang
authors.forename: Liansheng
authors.initials: L
authors.lastname: Zhong
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chen
keywords: chemokine; cytokine; receptor; urticaria
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