title: Growth inhibition and antioxidative status induced by selenium-enriched broccoli extract and selenocompounds in DNA mismatch repair-deficient human colon cancer cells.
abstract: The effects of enzymatic-digested Se-enriched broccoli extracts (SeB) and selenocompounds on growth and antioxidative status in human colon cancer cells was investigated in this study. HCT116 and HCT116+Chr.3 cells were treated with selenocompounds (sodium selenite, sodium selenate, Se-Met, MeSeCys) or SeB [high-Se (H-SeB) or low-Se (L-SeB)]. The cytotoxicity induced by selenocompounds in HCT116 cells was not associated with cellular H2O2 level, while the differential cytotoxicity observed by sodium selenite between HCT116 and HCT116+Chr.3 cell lines was related to cellular H2O2 production with the change in antioxidative enzyme activity, and the restoration of chromosome 3. H-SeB was found to reduce the cellular H2O2 content in HCT116+Chr.3 cells. The results in this study indicate that regardless of Se content, the cytotoxicity in HCT116 cells of both SeB forms appeared to be H2O2-independent, whereas the cytotoxicity in HCT116+Chr.3 of either SeB form appeared to be H2O2-dependent with an increase in antioxidative ability for H-SeB.
authors.affiliation: Department of Biotechnology, Asia University, Wu-Feng Chu, Taichung 413, Taiwan, ROC.
authors.forename: Cheng-Fang
authors.initials: CF
authors.lastname: Tsai
authors.forename: Bor-Rung
authors.initials: BR
authors.lastname: Ou
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Liang
authors.forename: Jan-Ying
authors.initials: JY
authors.lastname: Yeh
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D053843:DNA Mismatch Repair / Q000187:drug effects*; D045325:HCT116 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012643:Selenium / Q000494:pharmacology*; D018036:Selenium Compounds / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D018036:Selenium Compounds; D006861:Hydrogen Peroxide; D012643:Selenium