title: Kba.62 and S100 protein expression in cytologic samples of metastatic malignant melanoma.
abstract: The diagnosis of melanoma can be challenging, especially in metastatic lesions, due to the ability of melanoma cells to morphologically mimic carcinoma, sarcoma and even lymphoma cells. Moreover, melanomas can exhibit negative immunostaining for the melanoma markers HMB-45 and MART-1/Melan-A, often used in the diagnosis of this tumor. KBA.62 is a recently described antibody that reacts with benign and malignant melanocytic proliferations. In this study, we report our experience with KBA.62 and S100 protein immunostaining in the diagnosis of metastatic melanoma on fine-needle aspiration and effusion samples. We reviewed 60 cytology samples from 58 patients with metastatic melanoma. Our results showed that KBA.62 stained 75% of the cases and S100 protein 87% of the cases. KBA.62 and S100 protein stained the majority of metastatic melanomas that were negative for HMB-45 and MART-1; KBA.62 stained 73% of the cases and S100 protein 73% of the cases. The majority (85%) of the cases negative for HMB-45 and MART-1 were positive for KBA.62 and/or S100 protein. Additionally, we also observed that KBA.62 staining was positive in the majority of epithelioid and spindle cell type melanoma cells. In conclusion, the performances of KBA.62 and S100 protein were similar and both markers are useful in the diagnosis of metastatic melanoma in cytology material, especially when the tumor cells lack expression of HMB-45 and MART-1.
authors.affiliation: Cytopathology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
authors.forename: Gulsun
authors.initials: G
authors.lastname: Erdag
authors.forename: Sinchita Roy
authors.initials: SR
authors.lastname: Chowdhuri
authors.forename: Patricia
authors.initials: P
authors.lastname: Fetsch
authors.forename: Dana
authors.initials: D
authors.lastname: Erickson
authors.forename: Marybeth S
authors.initials: MS
authors.lastname: Hughes
authors.forename: Armando C
authors.initials: AC
authors.lastname: Filie
mesh_terms: D000911:Antibodies, Monoclonal; D044963:Biopsy, Fine-Needle; D016022:Case-Control Studies; D006801:Humans; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology / Q000556:secondary; D058950:Melanoma-Specific Antigens / Q000378:metabolism*; D009418:S100 Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; C588000:KBA.62 monoclonal antibody, human; D058950:Melanoma-Specific Antigens; D009418:S100 Proteins
keywords: KBA.62; S100 protein; cytology; immunocytochemistry/immunohistochemistry; melanoma
references.citation: Am J Surg Pathol. 2012 Feb;36(2):265-72
references.pmid: 22067329
references.citation: Mod Pathol. 1998 Aug;11(8):740-6
references.pmid: 9720502
references.citation: Diagn Cytopathol. 1991;7(4):380-6
references.pmid: 1935517
references.citation: Cancer. 1999 Feb 25;87(1):37-42
references.pmid: 10096358
references.citation: J Clin Pathol. 1995 Sep;48(9):826-31
references.pmid: 7490315
references.citation: Am J Surg Pathol. 2004 May;28(5):596-600
references.pmid: 15105646
references.citation: Virchows Arch. 2008 Feb;452(2):119-32
references.pmid: 18080139
references.citation: Am J Clin Pathol. 1988 Oct;90(4):385-90
references.pmid: 2459952
references.citation: Cancer Cytopathol. 2012 Feb 25;120(1):52-61
references.pmid: 21793228
references.citation: Am J Surg Pathol. 2002 Jan;26(1):82-7
references.pmid: 11756773
references.citation: Cytopathology. 1991;2(3):137-47
references.pmid: 1932577
references.citation: Hum Pathol. 2008 Aug;39(8):1136-42
references.pmid: 18495211
references.citation: Cesk Patol. 2004 Apr;40(2):50-6
references.pmid: 15233017
references.citation: Cytopathology. 2000 Oct;11(5):312-21
references.pmid: 11014658
references.citation: Am J Clin Pathol. 2007 Mar;127(3):385-97
references.pmid: 17276948
references.citation: Hum Pathol. 2005 Sep;36(9):1016-9
references.pmid: 16153466
references.citation: Acta Cytol. 1995 May-Jun;39(3):481-4
references.pmid: 7762336
references.citation: Diagn Cytopathol. 2009 Jul;37(7):516-21
references.pmid: 19301358
references.citation: Cancer. 1997 Feb 25;81(1):57-63
references.pmid: 9100543
references.citation: Am J Dermatopathol. 1994 Jun;16(3):233-40
references.pmid: 7943629
references.citation: Diagn Cytopathol. 1999 Jun;20(6):393-6
references.pmid: 10352917
references.citation: Am J Dermatopathol. 1997 Dec;19(6):580-4
references.pmid: 9415614
references.citation: Am J Surg Pathol. 2008 Mar;32(3):452-60
references.pmid: 18300804
references.citation: Cancer. 2008 Apr 25;114(2):94-101
references.pmid: 18260089
references.citation: Mod Pathol. 1998 Aug;11(8):699-703
references.pmid: 9720495
references.citation: Cancer. 1998 Apr 25;84(2):92-7
references.pmid: 9570211