title: Infection of primary hepatocytes with adenoviral vectors alters biliary lipid metabolism.
abstract: In the context of a study of the involvement of SND1 (also known as coactivator p100) in biliary lipid secretion by primary rat hepatocytes, first-generation adenoviral vectors were used to promote the overexpression and underexpression of the protein SND1. Although differential expression of SND1 did not result in significant changes in the processes studied, some effects of the adenoviral infection itself were observed. In particular, infected hepatocytes showed a higher intracellular taurocholate accumulation capacity. Additionally, small heterodimer partner (SHP) and farnesoid X receptor (FXR), which are nuclear receptors essential for the regulation of bile salt metabolism and transport, were underregulated at the mRNA level. Our results suggest that adenoviral vectors could be altering some important control mechanism and indicate that adenoviral vectors should be used with caution as transfection vectors for hepatocytes when biliary lipid metabolism is to be studied.
authors.affiliation: Department of Physiology, Faculty of Medicine and Dentistry of University of Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Rueda
authors.forename: Itsaso
authors.initials: I
authors.lastname: Garcia-Arcos
authors.forename: Patricia
authors.initials: P
authors.lastname: Aspichueta
authors.forename: Begoña
authors.initials: B
authors.lastname: Ochoa
authors.forename: Lourdes
authors.initials: L
authors.lastname: Palacios
authors.forename: Olatz
authors.initials: O
authors.lastname: Fresnedo
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D016375:Antisense Elements (Genetics); D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism*; D004720:Endonucleases; D005822:Genetic Vectors / Q000235:genetics*; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology*; D050356:Lipid Metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D051381:Rats
chemical_list: D016375:Antisense Elements (Genetics); D001647:Bile Acids and Salts; D009687:Nuclear Proteins; D004720:Endonucleases; C490639:Snd1 protein, rat
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