title: Sequence analysis of the whole genome of a recombinant Marek's disease virus strain, GX0101, with a reticuloendotheliosis virus LTR insert.
abstract: Marek's disease virus Chinese strain GX0101, isolated in 2001, is the first reported recombinant gallid herpesvirus type 2 (GaHV-2) field strain with one reticuloendotheliosis virus (REV) long terminal repeat (LTR) insert. We constructed an infectious bacterial artificial chromosome (BAC) clone of GX0101, which showed characteristics very similar to those of the parental virus in replication and pathogenicity. Using the GX0101 BAC clone, the complete genome of GX0101 was sequenced and analyzed. The length of the GX0101 genome is 178,101 bp, and it contains only one REV-LTR insert at a site 267 bp upstream of the sorf2 gene.
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Su
authors.forename: Ning
authors.initials: N
authors.lastname: Cui
authors.forename: Aijun
authors.initials: A
authors.lastname: Sun
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Li
authors.forename: Jiabo
authors.initials: J
authors.lastname: Ding
authors.forename: Zimeng
authors.initials: Z
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D016679:Genome, Viral; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D008380:Marek Disease / Q000821:virology*; D016296:Mutagenesis; D011201:Poultry Diseases / Q000821:virology; D011995:Recombination, Genetic; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D017422:Sequence Analysis, DNA; D020079:Terminal Repeat Sequences / Q000235:genetics*; D014779:Virus Replication