title: Natural phage nanoparticle-mediated real-time immuno-PCR for ultrasensitive detection of protein marker.
abstract: Current immuno-PCR methods either use bare or nanostructured DNA as a reporter or combine phage display for antigen binding and reporting, however, they are often complex to carry out and lack universality. We present a novel and universal design of immuno-PCR termed natural phage nanoparticle-mediated real-time immuno-PCR.
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Xu
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Huang
authors.forename: Changqing
authors.initials: C
authors.lastname: Yi
authors.forename: Tan
authors.initials: T
authors.lastname: Xiao
authors.forename: Qingge
authors.initials: Q
authors.lastname: Li
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D017123:Bacteriophage T7 / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000276:immunology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011506:Proteins; D016650:Fluorescein-5-isothiocyanate