title: γ-Aminobutyric acid B receptor immunoreactivity in the mouse adrenal medulla.
abstract: The present study examined gamma-aminobutyric acid B (GABAB ) receptor, GABA, choline acetyltransferase (ChAT), and neuronal nitric oxide synthase (nNOS) immunoreactivities in the mouse adrenal medulla. GABAB receptor immunoreactivity was seen in numerous chromaffin cells and in a few ganglion cells of the adrenal medulla. By using a formaldehyde-induced fluorescence (FIF) method, GABAB receptor immunoreactivity was observed in numerous adrenaline (A) cells, but not in noradrenaline (NA) cells showing blue-white fluorescence. This suggests that GABAB receptors may be present in the A cells and be related to the secretory activity of A cells but not NA cells in the mouse adrenal medulla. GABAB receptor immunoreactive ganglion cells were shown to be nNOS immunopositive by using a double immunostaining method. Weak GABA immunoreactivity was visible in some chromaffin cells and in the numerous nerve fibers of the medulla. By using the FIF method, weak GABA-immunoreactive chromaffin cells were shown to be in the NA cells showing blue-white fluorescence. GABA-immunoreactive nerve fibers were in dense contact in A cells, but not NA cells. GABA-immunoreactive nerve fibers closely contacted a few ganglion cells. Numerous GABA-immunoreactive nerve fibers in the medulla showed ChAT immunoreactive. This result suggests that GABA and acetylcholine may be released from the same nerve fibers and may have a secretory effect on the A cells of the medulla.
authors.affiliation: Division of Anatomy and Physiology, Japanese Red Cross Hokkaido College of Nursing, Kitami, Japan. oomori@rchokkaido-cn.ac.jp
authors.forename: Yukio
authors.initials: Y
authors.lastname: Oomori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murabayashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kuramoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kawano
authors.forename: Kanae
authors.initials: K
authors.lastname: Kato
authors.forename: Chieko
authors.initials: C
authors.lastname: Nakagawa
authors.forename: Motoki
authors.initials: M
authors.lastname: Sasaki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kitamura
authors.forename: Katsushi
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Kunio
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D018080:Receptors, GABA-B / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018080:Receptors, GABA-B; D005680:gamma-Aminobutyric Acid; D052248:Nitric Oxide Synthase Type I; D002795:Choline O-Acetyltransferase