title: Dummy-template molecularly imprinted solid phase extraction for selective analysis of ractopamine in pork.
abstract: Molecularly imprinted polymers (MIPs) for selective adsorption of ractopamine hydrochloride (RAC) were synthesised by an in situ method, in which salbutamol (SAL) was used as the dummy-template to avoid the template leakage. Scanning electron microscopy (SEM), mercury porosimerty and Fourier transform infrared spectroscopy (FTIR) were used to investigate the physical and morphological characteristics of the dummy-template MIPs. The test of adsorption selectivity indicated that the dummy-template MIPs exhibited high selectivity to RAC. The saturated adsorption capacity for RAC on dummy-template MIPs was 90.9 μg g(-1). Based on the dummy-template polymers, a liquid chromatography-mass spectrometry (LC-MS) method was developed for the selective analysis of RAC in real pork samples. The averages of intra- and inter-day accuracy ranged from 78.9% to 92.2% and from 90.7% to 93.1%, respectively. The RSD% of repeatability ranged from 1.9% to 6.3%, and the RSD% of intermediate precision ranged from 3.5% to 9.2%, while the limit of detection (LOD) was 0.02 μg kg(-1).
authors.affiliation: School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Jiao
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Luo
authors.forename: Chun
authors.initials: C
authors.lastname: Chang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D006133:Growth Substances / Q000737:chemistry / Q000302:isolation & purification*; D008460:Meat / Q000032:analysis*; D054802:Molecular Imprinting; D010627:Phenethylamines / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine
chemical_list: D006133:Growth Substances; D010627:Phenethylamines; D011108:Polymers; C060897:ractopamine