title: Fast isolation by AFLP of sequences containing repeats.
abstract: Fast isolation by AFLP of sequences containing repeats (FIASCO) is a rapid and simple method for separating microsatellite-containing DNA fragments from genomic DNA de novo. The method takes the advantage of the amplified fragment length polymorphism (AFLP) technique that relies on effective digestion-ligation reaction. The repeat-containing fragments are selectively hybridized to biotinylated probes and harvested by streptavidin-coated magnetic beads. The enriched microsatellite-containing fragments can be cloned and sequenced to yield a variety of microsatellite loci for applications in many different fields in molecular genetics.
authors.affiliation: Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.
authors.forename: Kaisa
authors.initials: K
authors.lastname: Rikalainen
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D001483:Base Sequence / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods; D009693:Nucleic Acid Hybridization
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D004247:DNA; C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific