title: In-stem labelling allows visualization of DNA strand displacements by distinct fluorescent colour change.
abstract: The combination of thiazole orange (TO) and thiazole red (TR) as an internal pair of fluorescent DNA base surrogates ("DNA traffic lights") allows us to follow at least two consecutive DNA strand displacements in real time through a distinct fluorescence colour change from green to red and vice versa.
authors.affiliation: Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Barrois
authors.forename: Hans-Achim
authors.initials: HA
authors.lastname: Wagenknecht
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D002232:Carbocyanines; D003116:Color; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D011804:Quinolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011804:Quinolines; C584189:thiazole red; C051000:thiazole orange; D004247:DNA