title: Influence of Kupffer cells and platelets on ischemia-reperfusion injury in mild steatotic liver.
abstract: OBJECTIVE
  To investigate the effect of mild steatotic liver on ischemia-reperfusion injury by focusing on Kupffer cells (KCs) and platelets.
  
  
  METHODS
  Wistar rats were divided into a normal liver group (N group) and a mild steatotic liver group (S group) induced by feeding a choline-deficient diet for 2 wk. Both groups were subjected to 20 min of warm ischemia followed by 120 min of reperfusion. The number of labeled KCs and platelets in sinusoids and the blood perfusion in sinusoids were observed by intravital microscopy (IVM), which was performed at 30, 60 and 120 min after reperfusion. To evaluate serum alanine aminotransferase as a marker of liver deterioration, blood samples were taken at the same time as IVM.
  
  
  RESULTS
  In the S group, the number of platelets adhering to KCs decreased significantly compared with the N group (120 after reperfusion; 2.9 ± 1.1 cells/acinus vs 4.8 ± 1.2 cells/acinus, P < 0.01). The number of KCs in sinusoids was significantly less in the S group than in the N group throughout the observation periods (before ischemia, 19.6 ± 3.3 cells/acinus vs 28.2 ± 4.1 cells/acinus, P < 0.01 and 120 min after reperfusion, 29.0 ± 4.3 cells/acinus vs 40.2 ± 3.3 cells/acinus, P < 0.01). The blood perfusion of sinusoids 120 min after reperfusion was maintained in the S group more than in the N group. Furthermore, elevation of serum alanine aminotransferase was lower in the S group than in the N group 120 min after reperfusion (99.7 ± 19.8 IU/L vs 166.3 ± 61.1 IU/L, P = 0.041), and histological impairment of hepatocyte structure was prevented in the S group.
  
  
  CONCLUSIONS
  Ischemia-reperfusion injury in mild steatotic liver was attenuated compared with normal liver due to the decreased number of KCs and the reduction of the KC-platelet interaction.
authors.affiliation: Department of Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305-8575, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ogawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tamura
authors.forename: Hideki
authors.initials: H
authors.lastname: Matsumura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Fukunaga
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Oda
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohkohchi
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism / Q000473:pathology; D002450:Cell Communication; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000097:blood / Q000473:pathology*; D015850:Interleukin-6 / Q000097:blood; D007728:Kupffer Cells / Q000378:metabolism / Q000473:pathology*; D008297:Male; D010973:Platelet Adhesiveness; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D000410:Alanine Transaminase
keywords: Intravital microscopy; Ischemia-reperfusion; Kupffer cell; Mild steatotic liver; Platelet; Steatotic liver
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