title: Calpains mediate axonal cytoskeleton disintegration during Wallerian degeneration.
abstract: In both the central nervous system (CNS) and peripheral nervous system (PNS), transected axons undergo Wallerian degeneration. Even though Augustus Waller first described this process after transection of axons in 1850, the molecular mechanisms may be shared, at least in part, by many human diseases. Early pathology includes failure of synaptic transmission, target denervation, and granular disintegration of the axonal cytoskeleton (GDC). The Ca(2+)-dependent protease calpains have been implicated in GDC but causality has not been established. To test the hypothesis that calpains play a causal role in axonal and synaptic degeneration in vivo, we studied transgenic mice that express human calpastatin (hCAST), the endogenous calpain inhibitor, in optic and sciatic nerve axons. Five days after optic nerve transection and 48 h after sciatic nerve transection, robust neurofilament proteolysis observed in wild-type controls was reduced in hCAST transgenic mice. Protection of the axonal cytoskeleton in sciatic nerves of hCAST mice was nearly complete 48 h post-transection. In addition, hCAST expression preserved the morphological integrity of neuromuscular junctions. However, compound muscle action potential amplitudes after nerve transection were similar in wild-type and hCAST mice. These results, in total, provide direct evidence that calpains are responsible for the morphological degeneration of the axon and synapse during Wallerian degeneration.
authors.affiliation: Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. marekma@mail.med.upenn.edu
authors.forename: Marek
authors.initials: M
authors.lastname: Ma
authors.forename: Toby A
authors.initials: TA
authors.lastname: Ferguson
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Schoch
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Yaping
authors.initials: Y
authors.lastname: Qian
authors.forename: Frances S
authors.initials: FS
authors.lastname: Shofer
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Saatman
authors.forename: Robert W
authors.initials: RW
authors.lastname: Neumar
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000502:physiology; D002154:Calpain / Q000502:physiology*; D002540:Cerebral Cortex / Q000473:pathology; D003599:Cytoskeleton / Q000473:pathology*; D004576:Electromyography; D055724:Electrophysiological Phenomena / Q000502:physiology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D016900:Neurofilament Proteins / Q000378:metabolism; D009469:Neuromuscular Junction / Q000473:pathology; D009900:Optic Nerve / Q000473:pathology; D012160:Retina / Q000473:pathology; D012584:Sciatic Nerve / Q000473:pathology; D014855:Wallerian Degeneration / Q000473:pathology*
chemical_list: D002135:Calcium-Binding Proteins; D016900:Neurofilament Proteins; C033668:calpastatin; D002154:Calpain
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