title: A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres.
abstract: We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.
authors.affiliation: Technology Division, Joint BioEnergy Institute, Emeryville, CA, USA. achandrasekaran@lbl.gov
authors.forename: Aarthi
authors.initials: A
authors.lastname: Chandrasekaran
authors.forename: Kai
authors.initials: K
authors.lastname: Deng
authors.forename: Chung-Yan
authors.initials: CY
authors.lastname: Koh
authors.forename: Taichi
authors.initials: T
authors.lastname: Takasuka
authors.forename: Lai F
authors.initials: LF
authors.lastname: Bergeman
authors.forename: Brian G
authors.initials: BG
authors.lastname: Fox
authors.forename: Paul D
authors.initials: PD
authors.lastname: Adams
authors.forename: Anup K
authors.initials: AK
authors.lastname: Singh
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism*; D008863:Microspheres; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011134:Polysaccharides; D016695:Glycosyltransferases; D006026:Glycoside Hydrolases