title: CD8(+) T cell activation by murine erythroblasts infected with malaria parasites.
abstract: Recent studies show that some human malaria parasite species Plasmodium falciparum and P. vivax parasitize erythroblasts; however, the biological and clinical significance of this is unclear. To investigate further, we generated a rodent malaria parasite (P. yoelii 17XNL) expressing GFP-ovalbumin (OVA). Its infectivity to erythroblasts was confirmed, and parasitized erythroblasts were capable of initiating malaria infections. Experiments showed that MHC class I molecules were highly expressed on parasitized erythroblasts. As CD8(+) T cells recognize MHC class I and peptide complexes on target cells, and are involved in protection or pathology against malaria, we examined whether erythroblasts are targeted by CD8(+) T cells. Purified non-parasitized erythroblasts pulsed with OVA peptides were recognized by OVA-specific CD8(+) T cells. Crucially, parasitized erythroblasts isolated from GFP-OVA-, but not GFP- infected-mice, activated OT-I CD8(+) T cells, indicating that CD8(+) T cells recognize parasitized erythroblasts in an antigen-specific manner.
authors.affiliation: Department of Parasitology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Imai
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Ishida
authors.forename: Kazutomo
authors.initials: K
authors.lastname: Suzue
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirai
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Okada
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Suzuki
authors.forename: Chikako
authors.initials: C
authors.lastname: Shimokawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Hisaeda
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004900:Erythroblasts / Q000276:immunology / Q000469:parasitology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology; D010967:Plasmodium yoelii / Q000276:immunology*
chemical_list: D015395:Histocompatibility Antigens Class I; D049452:Green Fluorescent Proteins; D010047:Ovalbumin
references.citation: Exp Hematol. 2002 Jul;30(7):634-9
references.pmid: 12135659
references.citation: Hematology Am Soc Hematol Educ Program. 2009;:87-93
references.pmid: 20008186
references.citation: Blood. 2001 Dec 1;98(12):3261-73
references.pmid: 11719363
references.citation: J Infect Dis. 2011 Dec 15;204(12):1989-96
references.pmid: 21998471
references.citation: Parasitol Res. 2012 Feb;110(2):913-23
references.pmid: 21842389
references.citation: Int J Parasitol. 2004 Dec;34(13-14):1501-16
references.pmid: 15582527
references.citation: Blood. 2004 May 15;103(10):3727-35
references.pmid: 14739226
references.citation: Trends Parasitol. 2012 Feb;28(2):73-82
references.pmid: 22100995
references.citation: Nature. 2005 Mar 10;434(7030):214-7
references.pmid: 15759000
references.citation: N Engl J Med. 2009 Jul 30;361(5):455-67
references.pmid: 19641202
references.citation: Nature. 1996 May 9;381(6578):166-8
references.pmid: 8610016
references.citation: J Immunol. 2010 May 1;184(9):4696-707
references.pmid: 20351188
references.citation: J Immunol. 2008 Jul 15;181(2):1420-8
references.pmid: 18606696
references.citation: Blood. 2009 Oct 22;114(17):3652-5
references.pmid: 19706885
references.citation: Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17413-8
references.pmid: 19805084
references.citation: Annu Rev Immunol. 2005;23:69-99
references.pmid: 15771566
references.citation: Ultrastruct Pathol. 2009 Oct;33(5):236-42
references.pmid: 19895296
references.citation: Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7167-72
references.pmid: 19346470
references.citation: Infect Immun. 1991 Jan;59(1):51-8
references.pmid: 1898902
references.citation: Br J Haematol. 2000 Dec;111(4):1010-22
references.pmid: 11167735
references.citation: Ann Trop Paediatr. 1988 Dec;8(4):250-8
references.pmid: 2467614
references.citation: Int J Parasitol. 2007 Dec;37(14):1551-7
references.pmid: 17610880
references.citation: Proc Natl Acad Sci U S A. 2011 Sep 27;108(39):16363-8
references.pmid: 21930907
references.citation: PLoS One. 2011 May 04;6(5):e19307
references.pmid: 21573240
references.citation: J Immunol Methods. 2004 Feb 1;285(1):25-40
references.pmid: 14871532
references.citation: Blood. 2000 Feb 15;95(4):1481-6
references.pmid: 10666228
references.citation: Int Rev Immunol. 2009;28(3-4):239-60
references.pmid: 19811323
references.citation: Cell Host Microbe. 2010 Mar 18;7(3):245-55
references.pmid: 20227667
references.citation: PLoS One. 2011;6(10):e26689
references.pmid: 22039533
references.citation: Immunol Lett. 1990 Aug;25(1-3):87-95
references.pmid: 2126529
references.citation: Nat Protoc. 2006;1(1):346-56
references.pmid: 17406255
references.citation: Blood. 2000 Apr 15;95(8):2559-68
references.pmid: 10753835
references.citation: J Immunol. 2005 Aug 1;175(3):1464-72
references.pmid: 16034083
references.citation: Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14509-14
references.pmid: 18799734
references.citation: Eur J Immunol. 2010 Apr;40(4):1053-61
references.pmid: 20101613