title: Potential contribution of SOCC to cerebral vasospasm after experimental subarachnoid hemorrhage in rats.
abstract: Cerebral vasospasm (CVS) is the most treatable component of subarachnoid hemorrhage (SAH), which can be reduced by endothelin receptor antagonists. Endothelin-evoked vasospasm is considered to be mediated by Ca(2+) influx in the smooth muscle through voltage-dependent Ca(2+) channel (VDCC) and nonselective cation channels (NSCC). Because VDCC antagonists such as nimodipine have been shown to be relatively less effective than the endothelin receptor antagonists, it is assumed that NSCC maybe a more important component in mediating Ca(2+) influx during CVS. In this study, we used the basilar arteries from a "two-hemorrhage" rat model of SAH to investigate expressions of transient receptor potential channel 1 (TRPC1), transient receptor potential channel 3 (TRPC3) and stromal interaction molecule 1 (STIM1), which are considered as the promising candidates constituting NSCC. To investigate the possible role of NSCC in phenotypic switching, we performed immunohistochemical staining to examine expressions of SMα-actin and PCNA, markers of smooth muscle phenotypic switching. We found that the basilar arteries exhibited vasospasm after SAH and that vasospasm became more severe on days 5 and 7 after SAH. Elevated mRNA and protein expressions of TRPC1 and STIM1 were detected after SAH and peaked on days 5 and 7, which was in a parallel time course to the development of cerebral vasospasm. The mRNA and protein expressions of TRPC3 were not changed in the SAH group when compared with those in the control. Results of immunohistochemical staining with anti-PCNA and anti-SMα-actin antibodies also showed enhanced expression of PCNA and disappearance of SMα-actin from day 1 to day 7. Taken together, the above results supported a novel mechanism that the components of store-operated calcium channels, TRPC1 and STIM1 mediated the Ca(2+) influx and phenotypic switching in smooth muscle cells, which promoted the development of vasospasm after SAH. TRPC3, which is a component of receptor-operated calcium channels, was not involved in the above-mentioned mechanism.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China. jinningsong@126.com
authors.forename: Jin-Ning
authors.initials: JN
authors.lastname: Song
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yan
authors.forename: Ji-Yang
authors.initials: JY
authors.lastname: An
authors.forename: Guang-Shan
authors.initials: GS
authors.lastname: Hao
authors.forename: Xiao-Ye
authors.initials: XY
authors.lastname: Guo
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Dan-Dong
authors.initials: DD
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
mesh_terms: D000199:Actins / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001488:Basilar Artery / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071737:Stromal Interaction Molecule 1; D013345:Subarachnoid Hemorrhage / Q000150:complications* / Q000473:pathology; D050052:TRPC Cation Channels / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D050051:Transient Receptor Potential Channels / Q000235:genetics / Q000378:metabolism*; D020301:Vasospasm, Intracranial / Q000209:etiology* / Q000378:metabolism*
chemical_list: D000199:Actins; D008562:Membrane Glycoproteins; D018809:Proliferating Cell Nuclear Antigen; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D050051:Transient Receptor Potential Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D002118:Calcium