title: Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma.
abstract: To evaluate the clinical significance of TLR3 expression on neuroblastomas, we performed immunohistochemical study on archival tissues and in vitro studies on neuroblastoma cell lines. The results showed that positive TLR3 expression was associated with favorable histology and prognosis. Activation of TLR3 by polyinosinic:polycytidylic acid [poly(I:C)] treatment is effective to suppress cell migration and invasion and to decrease organized assembly of F-actin and filopodia formation, in TLR3-expressing SK-N-AS cells, which could be reversed by TLR3-targeting siRNA treatment. TLR3 agonist poly(I:C) promotes GAP-43 expression also in SK-N-AS cells only. Taken together, TLR3 could serve to predict favorable behavior in neuroblastomas.
authors.affiliation: Department of Surgery, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Hsu
authors.forename: Chao-Cheng
authors.initials: CC
authors.lastname: Huang
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Wu
authors.forename: Hsinyu
authors.initials: H
authors.lastname: Lee
authors.forename: Min-Chuan
authors.initials: MC
authors.lastname: Huang
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Tai
authors.forename: Jiin-Haur
authors.initials: JH
authors.lastname: Chuang
mesh_terms: D000199:Actins / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D019922:GAP-43 Protein / Q000096:biosynthesis; D015870:Gene Expression; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D011070:Poly I-C / Q000494:pharmacology; D011379:Prognosis; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D015398:Signal Transduction; D051196:Toll-Like Receptor 3 / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000199:Actins; D019922:GAP-43 Protein; D034741:RNA, Small Interfering; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; D011070:Poly I-C