title: A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity.
abstract: A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 × 10(-6) U mL(-1), using a supersandwich amplification technique.
authors.affiliation: Key Laboratory of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, Xi'an, Shaanxi 710069, PR China. yanli@nwu.edu.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Luo
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yan
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zheng
authors.forename: Honglan
authors.initials: H
authors.lastname: Qi
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D015257:DNA-Cytosine Methylases / Q000032:analysis* / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry
chemical_list: D009942:Organometallic Compounds; D010618:Phenanthrolines; C034282:tris-(1,10-phenanthroline)ruthenium; D004247:DNA; C029641:DNA modification methylase HpaII; C081573:DNA modification methylase SssI; D015257:DNA-Cytosine Methylases