title: Pharmacokinetic studies of bergapten in dog plasma by using a LC-MS/MS method studies.
abstract: A sensitive LC-MS/MS method was developed for the determination of bergapten in dog plasma. The chromatographic separation was carried out on a Hypersil ODS column with a mobile phase consisting of methanol-water. The plasma sample was precipitated with methanol and prepare for injecting onto the LC-MS/MS system. The detection was performed on a triple quadrupole tandem mass spectrometer by MRM via electro spray ionization source. The standard curve for bergapten was linear over the concentration range of 0.5-500 ng/mL with a lower limit of quantification of 0.5 ng/mL. The inter-day and intra-day precision (R.S.D.%) for bergapten varied between 3.4 and 11.5. The corresponding inter-day and intra-day accuracy (Bias%) ranged between -3.8 and 6.9. For the pharmacokinetic analysis of serum, the mean (SD) values obtained for the bergapten were as follows: Cmax, 228.5 (14.3) ng/ml; Tmax, 4.2 (0.4) h; t1/2, 6.9 (2.3) h; AUC0-t h, 2507.2 (168.5) ng · h/mL and AUC0-∞, 3 219.2 (211.4) ng · h/mL, respectively.
authors.affiliation: Department of Oncology, Shanghai East Hospital, Tongji University, Shanghai China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Liu
authors.forename: X-M
authors.initials: XM
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: C-Y
authors.initials: CY
authors.lastname: Dong
mesh_terms: D000078223:5-Methoxypsoralen; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D006207:Half-Life; D057230:Limit of Detection; D008297:Male; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000078223:5-Methoxypsoralen; D008730:Methoxsalen