title: Synthesis of oleic acid functionalized Fe3O4 magnetic nanoparticles and studying their interaction with tumor cells for potential hyperthermia applications.
abstract: In the present study, oleic acid (OA) functionalized Fe3O4 magnetic nanoparticles (MN) were synthesized following modified wet method of MN synthesis. The optimum amount of OA required for capping of MN and the amount of bound and unbound/free OA was determined by thermogravimetric analysis (TGA). Further, we have studied the effect of water molecules, associated with MN, on the variation in their induction heating ability under alternating current (AC) magnetic field conditions. We have employed a new approach to achieve dispersion of OA functionalized MN (MN-OA) in aqueous medium using sodium carbonate, which improves their biological applicability. Interactions amongst MN, OA and sodium carbonate were studied by Fourier transform infrared spectroscopy (FT-IR). Intracellular localization of MN-OA was studied in mouse fibrosarcoma cells (WEHI-164) by prussian blue staining and confocal laser scanning microscopy (CLSM) using nile blue A as a fluorescent probe. Results showed MN-OA to be interacting mainly with the cell membrane. Their hyperthermic killing ability was evaluated in WEHI-164 cells by trypan blue method. Cells treated with MN-OA in combination with induction heating showed decreased viability as compared to respective induction heating controls. These results were supported by altered cellular morphology after treatment of MN-OA in combination with induction heating. Further, the magnitude of apoptosis was found to be ~5 folds higher in cells treated with MN-OA in combination with induction heating as compared to untreated control. These results suggest the efficacy of MN-OA in killing of tumor cells by cellular hyperthermia.
authors.affiliation: Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Neena V
authors.initials: NV
authors.lastname: Jadhav
authors.forename: Amresh I
authors.initials: AI
authors.lastname: Prasad
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.forename: R
authors.initials: R
authors.lastname: Mishra
authors.forename: Sangita
authors.initials: S
authors.lastname: Dhara
authors.forename: K R
authors.initials: KR
authors.lastname: Babu
authors.forename: C L
authors.initials: CL
authors.lastname: Prajapat
authors.forename: N L
authors.initials: NL
authors.lastname: Misra
authors.forename: R S
authors.initials: RS
authors.lastname: Ningthoujam
authors.forename: B N
authors.initials: BN
authors.lastname: Pandey
authors.forename: R K
authors.initials: RK
authors.lastname: Vatsa
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002254:Carbonates / Q000737:chemistry*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006361:Heating; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D019301:Oleic Acid / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014867:Water
chemical_list: D000970:Antineoplastic Agents; D002254:Carbonates; D058185:Magnetite Nanoparticles; D014867:Water; D019301:Oleic Acid; C005686:sodium carbonate; D052203:Ferrosoferric Oxide