title: Improvement of mammalian cell culture performance through surfactant enabled concentrated feed media.
abstract: The design of basal and feed media in mammalian cell culture is paramount towards ensuring acceptable upstream process performance in various operation modes, especially fed-batch culture. Mammalian cell culture media designs have evolved from the classical formulations designed by Eagle and Ham, to today's formulations designed from continuous improvement and statistical frameworks. Feed media is especially important for ensuring robust cell growth, productivity, and ensuring the product quality of recombinant therapeutics are within acceptable ranges. Numerous studies have highlighted the benefit of various media designs, supplements, and feed addition strategies towards the resulting cell culture process. In this work we highlight the use of a top-down level approach towards feed media design enabled by the use of select surfactants for the targeted enrichment of a chemically defined feed media. The use of the enriched media was able to improve product titers at g/L levels, without adversely impacting the growth of multiple Chinese Hamster Ovary cell lines or the product quality of multiple recombinant antibodies.
authors.affiliation: Process Sciences, AbbVie Bioresearch Center, AbbVie Inc., Worcester, MA 01605, USA. patrick.hossler@abbvie.com
authors.forename: Patrick
authors.initials: P
authors.lastname: Hossler
authors.forename: Sean
authors.initials: S
authors.lastname: McDermott
authors.forename: Christopher
authors.initials: C
authors.lastname: Racicot
authors.forename: John C H
authors.initials: JC
authors.lastname: Fann
mesh_terms: D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D003412:Cricetulus; D017077:Culture Media, Conditioned / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D011136:Polysorbates; D013501:Surface-Active Agents; D020442:Poloxamer
keywords: chemically defined media; mammalian cell culture; polysorbate 80; surfactants