title: [The management of the metastatic disease by the surgeons].
abstract: The high rate of recurrence after apparently complete surgery and/or complete clinical response to chemotherapy implies that most patients have undetected minimal residual disease. Novel techniques such as laparoscopic or laparotomic fluorescence may prove to be crucial in reassessing the definition of primary outcome in ovarian cancer management. For this purpose, the importance of fluorescence in the peroperative detection of human ovarian adenocarcinoma cells has to be validated in animal models prior to be used in clinic by determining its efficiency in detecting infra-millimetric tumor metastases. In the preclinical data presented, a fluorescent RAFT-(cRGD)4 tracer molecule (AngioStamp(®)) with a very high affinity for the αvβ3 integrin was used. Infrared fluorescence was visualized with Fluobeam(®), an open fluorescent imaging system that could potentially be used in peroperative conditions in the future. We showed that this novel technique allowed the specific detection of residual tumor deposits and inframillimetric metastases in mice and dogs.
authors.affiliation: Faculté Toulouse-III, Institut Claudius-Regaud, 20-24 rue du Pont-Saint-Pierre, Toulouse, France.
authors.forename: Eliane
authors.initials: E
authors.lastname: Mery
authors.forename: Muriel
authors.initials: M
authors.lastname: Golzio
authors.forename: Benoît
authors.initials: B
authors.lastname: Thibault
authors.forename: Gwenael
authors.initials: G
authors.lastname: Ferron
authors.forename: Denis
authors.initials: D
authors.lastname: Querleu
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Delord
authors.forename: Bettina
authors.initials: B
authors.lastname: Couderc
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000032:analysis*; D051379:Mice; D061206:Neoplasm Micrometastasis / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis*; D009364:Neoplasm Recurrence, Local; D018365:Neoplasm, Residual; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods
chemical_list: D039302:Integrin alphaVbeta3; D009363:Neoplasm Proteins
keywords: fluorescent imaging; integrins; metastasis; ovarain cancer; surgery