title: Clonal development and organization of the adult Drosophila central brain.
abstract: BACKGROUND
  The insect brain can be divided into neuropils that are formed by neurites of both local and remote origin. The complexity of the interconnections obscures how these neuropils are established and interconnected through development. The Drosophila central brain develops from a fixed number of neuroblasts (NBs) that deposit neurons in regional clusters.
  
  
  RESULTS
  By determining individual NB clones and pursuing their projections into specific neuropils, we unravel the regional development of the brain neural network. Exhaustive clonal analysis revealed 95 stereotyped neuronal lineages with characteristic cell-body locations and neurite trajectories. Most clones show complex projection patterns, but despite the complexity, neighboring clones often coinnervate the same local neuropil or neuropils and further target a restricted set of distant neuropils.
  
  
  CONCLUSIONS
  These observations argue for regional clonal development of both neuropils and neuropil connectivity throughout the Drosophila central brain.
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Hung-Hsiang
authors.initials: HH
authors.lastname: Yu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Awasaki
authors.forename: Mark David
authors.initials: MD
authors.lastname: Schroeder
authors.forename: Fuhui
authors.initials: F
authors.lastname: Long
authors.forename: Jacob S
authors.initials: JS
authors.lastname: Yang
authors.forename: Yisheng
authors.initials: Y
authors.lastname: He
authors.forename: Peng
authors.initials: P
authors.lastname: Ding
authors.forename: Jui-Chun
authors.initials: JC
authors.lastname: Kao
authors.forename: Gloria Yueh-Yi
authors.initials: GY
authors.lastname: Wu
authors.forename: Hanchuan
authors.initials: H
authors.lastname: Peng
authors.forename: Gene
authors.initials: G
authors.lastname: Myers
authors.forename: Tzumin
authors.initials: T
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D019070:Cell Lineage; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D005260:Female; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D019581:Neuropil / Q000166:cytology / Q000378:metabolism
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