title: The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients.
abstract: The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.
authors.affiliation: Faculty of Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Katia Cristina
authors.initials: KC
authors.lastname: Dantas
authors.forename: Roseli S
authors.initials: RS
authors.lastname: Freitas
authors.forename: Adriana Pardini Vicentini
authors.initials: AP
authors.lastname: Moreira
authors.forename: Marcos Vinicius da
authors.initials: MV
authors.lastname: Silva
authors.forename: Gil
authors.initials: G
authors.lastname: Benard
authors.forename: Cidia
authors.initials: C
authors.lastname: Vasconcellos
authors.forename: Paulo Ricardo
authors.initials: PR
authors.lastname: Criado
mesh_terms: D042241:Early Diagnosis; D006679:HIV Seropositivity / Q000097:blood*; D006658:Histoplasma / Q000235:genetics* / Q000302:isolation & purification; D006660:Histoplasmosis / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
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