title: [Molecular detection of hepatitis E virus in pig livers destined for human consumption in the state of Nuevo Leon, Mexico].
abstract: OBJECTIVE
  Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico.
  
  
  METHODS
  87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR.
  
  
  RESULTS
  19.54% (17) of tif's and 22.5% (9) of butcher's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3.
  
  
  CONCLUSIONS
  Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.
authors.affiliation: Departamento de Virología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, Nuevo León, México.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Cantú-Martínez
authors.forename: Artur Xavier
authors.initials: AX
authors.lastname: Roig-Sagués
authors.forename: Sibilina
authors.initials: S
authors.lastname: Cedillo-Rosales
authors.forename: Diana Elisa
authors.initials: DE
authors.lastname: Zamora-Ávila
authors.forename: Ramiro
authors.initials: R
authors.lastname: Avalos-Ramírez
mesh_terms: D000818:Animals; D019649:Food Industry; D016752:Hepatitis E virus / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000821:virology*; D008800:Mexico; D025202:Molecular Diagnostic Techniques; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine