title: Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex.
abstract: The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 Å.
authors.affiliation: Molecular Structural Biology, Georg-August-University Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
authors.forename: Peter Thomas
authors.initials: PT
authors.lastname: Naumann
authors.forename: Charles T
authors.initials: CT
authors.lastname: Lauhon
authors.forename: Ralf
authors.initials: R
authors.lastname: Ficner
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D008025:Ligases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D020124:Thermotoga maritima / Q000201:enzymology*; D013891:Thiouridine / Q000378:metabolism
chemical_list: D013891:Thiouridine; D012313:RNA; D008025:Ligases
keywords: 4-thiouridine; protein–RNA complex
references.citation: Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):282-92
references.pmid: 21460446
references.citation: Nucleic Acids Res. 2010 Jul;38(12):4154-62
references.pmid: 20203084
references.citation: Chem Commun (Camb). 2006 Aug 7;(29):3104-6
references.pmid: 16855700
references.citation: Nucleic Acids Res. 2013 Jan;41(Database issue):D262-7
references.pmid: 23118484
references.citation: J Mol Biol. 1998 Dec 4;284(3):621-31
references.pmid: 9826503
references.citation: Biochemistry. 2010 Jun 22;49(24):4934-44
references.pmid: 20459084
references.citation: J Biol Chem. 2001 Sep 7;276(36):33588-95
references.pmid: 11443125
references.citation: Chembiochem. 2008 Aug 11;9(12):1879-82
references.pmid: 18604845
references.citation: FEBS Lett. 1999 Jun 4;452(1-2):47-51
references.pmid: 10376676
references.citation: J Biol Chem. 2002 May 10;277(19):16391-5
references.pmid: 11861649
references.citation: J Biomol Struct Dyn. 1987 Dec;5(3):669-87
references.pmid: 3078237
references.citation: Biochem Biophys Res Commun. 1988 Feb 15;150(3):979-86
references.pmid: 2449211
references.citation: Eur J Biochem. 1980 Dec;113(1):67-74
references.pmid: 7007049
references.citation: J Mol Biol. 1978 Sep 25;124(3):523-34
references.pmid: 361973
references.citation: J Biol Chem. 2000 Apr 14;275(15):10727-30
references.pmid: 10753862
references.citation: J Bacteriol. 2012 Sep;194(18):4933-40
references.pmid: 22773787
references.citation: Proc Natl Acad Sci U S A. 1976 Jan;73(1):59-63
references.pmid: 1108019
references.citation: RNA. 2009 Aug;15(8):1498-506
references.pmid: 19509301
references.citation: J Bacteriol. 2002 Dec;184(24):6820-9
references.pmid: 12446632
references.citation: Nucleic Acids Res. 1999 Jan 1;27(1):196-7
references.pmid: 9847178
references.citation: Nucleic Acids Res. 1998 Jun 1;26(11):2606-10
references.pmid: 9592144
references.citation: RNA. 1999 Feb;5(2):188-94
references.pmid: 10024171
references.citation: J Biol Chem. 2004 May 28;279(22):23022-9
references.pmid: 15037613
references.citation: Nucleic Acids Res. 1997 Mar 15;25(6):1272-80
references.pmid: 9092639
references.citation: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt 1):56-8
references.pmid: 17183176
references.citation: J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
references.pmid: 19461840
references.citation: J Mol Biol. 2006 Feb 10;356(1):97-110
references.pmid: 16343540
references.citation: J Mol Biol. 1971 May 28;58(1):367-79
references.pmid: 4932656
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707