title: Acute peripheral GLP-1 receptor agonism or antagonism does not alter energy expenditure in rats after Roux-en-Y gastric bypass.
abstract: Compared to traditional weight loss strategies, the compensatory decrease in energy expenditure in response to body weight loss is markedly attenuated after Roux-en-Y gastric bypass surgery (RYGB). Because basal and postprandial levels of glucagon-like peptide-1 (GLP-1) are increased after RYGB surgery, and because GLP-1 has been shown to increase energy expenditure, we investigated if increased GLP-1 levels are involved in the alterations in energy expenditure after RYGB. Adult male Wistar rats were randomized for RYGB (n=8) or sham surgery (n=17). Part of the sham-operated rats were food restricted and body weight-matched (n=8) to the RYGB animals. The effects of acute subcutaneous administration of the GLP-1 antagonist Exendin (9-39) (Ex-9, 30μg/kg) or the GLP-1 agonist Exendin-4 (Ex-4, 5μg/kg), respectively, on energy expenditure were tested using indirect calorimetry. We found that Ex-9 increased food intake in RYGB, but not in sham-operated rats. Energy expenditure was lower in RYGB and sham-operated body weight-matched rats compared to sham-operated ad libitum fed rats, but significantly higher in RYGB rats compared to sham-operated body weight-matched rats. There was no effect of Ex-9 treatment on energy expenditure in either group of animals. Similarly, Ex-4 decreased food intake more in RYGB than in sham-operated rats, but Ex-4 did not modulate energy expenditure in any surgical group. We conclude that acute modulation of GLP-1 signaling is not directly involved in altered energy expenditure after RYGB surgery in rats.
authors.affiliation: Institute of Veterinary Physiology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Abegg
authors.forename: Marc
authors.initials: M
authors.lastname: Schiesser
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Lutz
authors.forename: Marco
authors.initials: M
authors.lastname: Bueter
mesh_terms: D000204:Activity Cycles; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002151:Calorimetry; D004435:Eating / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects*; D000077270:Exenatide; D005508:Food Deprivation; D015390:Gastric Bypass; D000067757:Glucagon-Like Peptide-1 Receptor; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D010446:Peptide Fragments / Q000008:administration & dosage*; D010455:Peptides / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D018027:Receptors, Glucagon / Q000819:agonists* / Q000037:antagonists & inhibitors*; D012119:Respiration / Q000187:drug effects; D013997:Time Factors; D014688:Venoms / Q000008:administration & dosage*
chemical_list: C000593835:Glp1r protein, rat; D000067757:Glucagon-Like Peptide-1 Receptor; D010446:Peptide Fragments; D010455:Peptides; D018027:Receptors, Glucagon; D014688:Venoms; C083773:exendin (9-39); D000077270:Exenatide
keywords: Energy expenditure; Glucagon-like peptide-1; Indirect calorimetry; Roux-en-Y gastric bypass
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