title: [Effects of rhAm on attachment, proliferation and immigration of human fibroblasts].
abstract: OBJECTIVE
  To compare the effects of 25 kDa full-length rhAm and porcine EMPs on cell behaviors of human periodontal ligament fibroblasts (HPDLF) and foreskin fibroblasts(HFF).
  
  
  METHODS
  rhAm was induced by BL21/pET28a-His-SUMO-rhAm express system, and 25 kDa full-length rhAm was analyzed by SDS-PAGE and Western blot. EMPs were extracted by acetic acid method. HPDLF and HFF were cultured in vitro. The cells were treated with rhAm and EMPs at different concentrations. The cell adhesion, proliferation and migration assays were qualitatively analyzed. The data was statistically analyzed with SAS 5.0 software package.
  
  
  RESULTS
  10-20 μg/mL rhAm significantly promoted the adhesion, proliferation and migration of HPDLF and HFF (P<0.05), but no significant difference between two proteins was found (P>0.05).
  
  
  CONCLUSIONS
  25 kDa rhAm and EMPs shows similar biological effects on fibroblast, which indicates that rhAm may play an important role in the periodontal regeneration through the activation of fibroblasts.
authors.affiliation: Department of Periodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. zhklin8744@yahoo.cn
authors.forename: Zhi-kai
authors.initials: ZK
authors.lastname: Lin
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.forename: Zhong-chen
authors.initials: ZC
authors.lastname: Song
authors.forename: Qiu-man
authors.initials: QM
authors.lastname: Guo
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D005347:Fibroblasts; D006801:Humans; D010513:Periodontal Ligament; D013552:Swine