title: Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells.
abstract: Less is known about the non-mesenchymal mononuclear cell fraction of human bone marrow on functional adaptation of neuroblastoma cells. Using immunocytochemistry, we showed that bone-marrow mononuclear cell (BMMC)-conditioned medium can induce tyrosine hydroxylase expression in neuroblastoma cells, which is similar to the effect of retinoic acid. Using quantitative RT-PCR, we showed that NGF, CNTF, and BDNF mRNAs were detected in unfractionated BMMC populations from all human donors at different expression levels. Our results suggest that cells of the non-mesenchymal mononuclear cell fraction can induce functional adaptation of neuroblastoma cells, probably via their secreted trophic factors.
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand. permphan.dha@mahidol.ac.th.
authors.forename: Chareerut
authors.initials: C
authors.lastname: Phruksaniyom
authors.forename: Permphan
authors.initials: P
authors.lastname: Dharmasaroja
authors.forename: Surapol
authors.initials: S
authors.lastname: Issaragrisil
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