title: TRACER: a resource to study the regulatory architecture of the mouse genome.
abstract: BACKGROUND
  Mammalian genes are regulated through the action of multiple regulatory elements, often distributed across large regions. The mechanisms that control the integration of these diverse inputs into specific gene expression patterns are still poorly understood. New approaches enabling the dissection of these mechanisms in vivo are needed.
  
  
  RESULTS
  Here, we describe TRACER (http://tracerdatabase.embl.de), a resource that centralizes information from a large on-going functional exploration of the mouse genome with different transposon-associated regulatory sensors. Hundreds of insertions have been mapped to specific genomic positions, and their corresponding regulatory potential has been documented by analysis of the expression of the reporter sensor gene in mouse embryos. The data can be easily accessed and provides information on the regulatory activities present in a large number of genomic regions, notably in gene-poor intervals that have been associated with human diseases.
  
  
  CONCLUSIONS
  TRACER data enables comparisons with the expression pattern of neighbouring genes, activity of surrounding regulatory elements or with other genomic features, revealing the underlying regulatory architecture of these loci. TRACER mouse lines can also be requested for in vivo transposition and chromosomal engineering, to analyse further regions of interest.
authors.affiliation: European Bioinformatics Institute - European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
authors.forename: Chao-Kung
authors.initials: CK
authors.lastname: Chen
authors.forename: Orsolya
authors.initials: O
authors.lastname: Symmons
authors.forename: Veli Vural
authors.initials: VV
authors.lastname: Uslu
authors.forename: Taro
authors.initials: T
authors.lastname: Tsujimura
authors.forename: Sandra
authors.initials: S
authors.lastname: Ruf
authors.forename: Damian
authors.initials: D
authors.lastname: Smedley
authors.forename: François
authors.initials: F
authors.lastname: Spitz
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D030541:Databases, Genetic; D016678:Genome; D020407:Internet; D051379:Mice; D014584:User-Computer Interface
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