title: A novel antithrombotic coronary stent: lysine-poly(HEMA)-modified cobalt-chromium stent with fibrinolytic activity.
abstract: Prevention of coagulation appears not to be possible when a foreign surface is in contact with blood; the alternative concept of a clot lysing surface has therefore been suggested. In this work, a mimic of the fibrinolytic system was constructed on L605 cobalt-chromium coronary stents. Lysine which, immobilized on a surface, has been shown previously to adsorb plasminogen in contact with blood was attached to the stent using poly(2-hydroxyethyl methacrylate) (poly(HEMA)) as a spacer. The lysine-poly(HEMA) modified stent was shown to have low nonspecific protein adsorption and to bind plasminogen in high quantity from plasma. Following exposure to plasma and treatment with tissue plasminogen activator, the lysine-modified stents showed clot-lysing properties in vitro while the unmodified L605 stents did not. It was shown that the modified stents retained their clot lysing properties after 24 h exposure to plasma.
authors.affiliation: Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Wu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Xu
authors.forename: John L
authors.initials: JL
authors.lastname: Brash
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D002857:Chromium / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000737:chemistry / Q000494:pharmacology*; D010958:Plasminogen / Q000302:isolation & purification / Q000378:metabolism*; D011073:Polyamines / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000737:chemistry; D011485:Protein Binding; D015607:Stents
chemical_list: D000925:Anticoagulants; D011073:Polyamines; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D002857:Chromium; D011102:Polyhydroxyethyl Methacrylate; D003035:Cobalt; D010958:Plasminogen; D008239:Lysine