title: RNAi-mediated MMP-9 silencing inhibits mouse melanoma cell invasion and migration in vitro and in vivo.
abstract: MMP-9 participates in tumour growth, invasion, metastasis and vascularisation. Thus, inhibition of MMP-9 may be involved in the process of tumourigenesis. We have investigated the effect of RNAi-mediated MMP-9 silencing on inhibiting invasion and migration of mouse melanoma cell B16. A specific and optimised siRNA vector was used to target MMP-9 mRNA synthesis in B16 cells. Changes of invasion and migration capability of B16 cell were examined after transfection at different time, and a footpad tumour model was performed to measure the effect of MMP-9 siRNA on melanoma tumourigenesis in vivo. In vitro, down-regulation of MMP-9 expression significantly inhibited B16 cell invasion and migration. In vivo, intratumoural injection of plasmid DNA expressing MMP-9 siRNA every 3 days for three times remarkably inhibited melanoma growth and also suppressed tumour metastasis. The results indicate that RNA-mediated targeting of MMP-9 may have promising applications for the treatment of melanoma.
authors.affiliation: College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Zhao-Yong
authors.initials: ZY
authors.lastname: Tang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Long-Xing
authors.initials: LX
authors.lastname: Liu
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Ding
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Liao-Qiong
authors.initials: LQ
authors.lastname: Fang
mesh_terms: D000818:Animals; D002465:Cell Movement; D055785:Gene Knockdown Techniques; D008175:Lung Neoplasms / Q000201:enzymology* / Q000556:secondary; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D008546:Melanoma, Experimental / Q000201:enzymology* / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D047368:Tumor Burden
chemical_list: D034741:RNA, Small Interfering; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse
keywords: MMP-9; anti-cancer therapy; invasion; melanoma; metastasis; siRNA; tumour growth