title: Prevalence of porcine enterovirus 9 in pigs in middle and eastern China.
abstract: Little information on the epidemiology and pathogenicity of porcine enterovirus 9 (PEV-9) is available. The present study investigated the prevalence of PEV-9 in pig populations in middle and eastern China using reverse transcriptase (RT)-PCR. All 14 sampled farms were positive for PEV-9 and the overall prevalence of infection in the studied pigs was 8.3% (37/447). There was a higher frequency of infection in pigs aged 10-15 weeks (12/119, 10.1%) than in pigs aged >20 weeks (5/103, 4.9%). A 313 nucleotide sequence from the 5'-UTR region of 37 Chinese PEV-9 positive samples had 96.1-100% sequence homology. On phylogenetic analysis, sequences clustered into two major groups, from which two representative strains were selected to determine the complete RNA-dependent RNA polymerase (RdRp) gene sequence. Phylogenetic analysis based on the RdRp gene suggested that PEV-9 strains from China formed a new subgroup. Piglets were inoculated orally with the PEV-9 strain identified in this study. Although most experimental pigs showed no clinical signs, almost all carried PEV-9 in one or more tissues after 6 days post-inoculation. The results of tissue histologic examination suggested that PEV9 can cause pathological changes in cerebrum and lung.
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu, PR China.
authors.forename: Shixing
authors.initials: S
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D054022:Cerebrum / Q000473:pathology / Q000821:virology; D002681:China; D016000:Cluster Analysis; D004769:Enterovirus Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004771:Enteroviruses, Porcine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014644:Genetic Variation; D005838:Genotype; D008168:Lung / Q000473:pathology / Q000821:virology; D010802:Phylogeny; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017385:Sequence Homology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D014764:Viral Proteins
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