title: Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol.
abstract: To enhance the value-added use of methanol-containing raw glycerol derived from biodiesel fuel production, the effect of methanol supplementation on glyceric acid (GA) production by Gluconobacter spp. was investigated. We first conducted fed-batch fermentation with Gluconobacter frateurii NBRC103465 using raw glycerol as a feeding solution. GA productivity decreased with increasing dihydroxyacetone (DHA) formation when the raw glycerol contained methanol. The results of this experiment and comparative experiments using a synthetic solution modeled after the raw glycerol indicate that the presence of methanol caused a change in the concentrations of GA and DHA, two glycerol derivatives produced during fermentation. Other Gluconobacter spp. also decreased GA production in the presence of 1% (v/v) methanol. In addition, purified membrane-bound alcohol dehydrogenase (mADH) from Gluconobacter oxydans, which is a key enzyme in GA production, showed a decrease in dehydrogenase activity toward glycerol as the methanol concentration increased. These results strongly suggest that the observed decrease in GA production by Gluconobacter spp. resulted from the methanol-induced inhibition of mADH-mediated glycerol oxidation.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan. hiroshi.habe@aist.go.jp.
authors.forename: Shun
authors.initials: S
authors.lastname: Sato
authors.forename: Naoki
authors.initials: N
authors.lastname: Morita
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
references.citation: Biotechnol Bioeng. 2009 Nov 1;104(4):729-39
references.pmid: 19575416
references.citation: Appl Microbiol Biotechnol. 2009 Jan;81(6):1033-9
references.pmid: 18853153
references.citation: Biosci Biotechnol Biochem. 2008 Mar;72(3):842-50
references.pmid: 18323643
references.citation: Appl Environ Microbiol. 2009 Dec;75(24):7760-6
references.pmid: 19837846
references.citation: J Oleo Sci. 2011;60(6):327-31
references.pmid: 21606621
references.citation: Appl Microbiol Biotechnol. 2009 Sep;84(3):445-52
references.pmid: 19621222
references.citation: Bioresour Technol. 2011 Jul;102(14):7177-82
references.pmid: 21592784
references.citation: Biosci Biotechnol Biochem. 2005 Jun;69(6):1120-9
references.pmid: 15973043
references.citation: Biosci Biotechnol Biochem. 2009 Aug;73(8):1799-805
references.pmid: 19661679
references.citation: J Oleo Sci. 2011;60(11):585-90
references.pmid: 22027025
references.citation: J Oleo Sci. 2011;60(7):369-73
references.pmid: 21701101
references.citation: J Oleo Sci. 2012;61(6):343-8
references.pmid: 22687780
references.citation: Metabolism. 2007 Jul;56(7):895-8
references.pmid: 17570248
references.citation: Biosci Biotechnol Biochem. 2010;74(7):1391-5
references.pmid: 20622460