title: lnterleukin-8 gene polymorphism and susceptibility to gastric cancer in a Brazilian population.
abstract: BACKGROUND
  Studies have demonstrated that some polymorphisms in different interleukin genes may increase the risk of cancer. The aim of this study was to investigate the association between the IL-8 (rs4073) -251A/T gene polymorphism and the risk of gastric cancer (GC).
  
  
  METHODS
  A case-control study was conducted on patients with noncardia gastric cancer. DNA was extracted from leukocytes and the IL-8 (rs4073) -251A/T polymorphism was analyzed by PCR-RFLP. Infection with Helicobacter pylori was investigated in the serum by ELISA.
  
  
  RESULTS
  The sample consisted of 104 patients with GC and 196 controls. Cigarette smoking (P=0.007) and high fat intake (P=0.01) were more frequent in patients with GC. The proportion of patients infected with H. pylori was similar in the two groups (P=0.101). The frequency of the genotype A/T was higher in the cancer group (P=0.008). An increased risk of GC was found in subjects carrying the genotype A/T (OR=2.50, CI: 1.27-4.90), subjects with high fat intake (OR=1.92, CI: 1.17-3.15), and smokers (OR=2.00, CI: 1.203.31).
  
  
  CONCLUSIONS
  Subjects with the heterozygous A/T genotype, high fat intake and smokers or ex-smokers presented an increased risk of GC. Individuals with A/A genotype may have protective effect for GC.
authors.affiliation: Grupo de Oncologia, Disciplina de Gastroenterologia, Universidade Federal de Sao Paulo, SP, Brasil. aledson.felipe@gmail.com
authors.forename: Aledson V
authors.initials: AV
authors.lastname: Felipe
authors.forename: Tiago D
authors.initials: TD
authors.lastname: Silva
authors.forename: Célia A
authors.initials: CA
authors.lastname: Pimenta
authors.forename: Paulo
authors.initials: P
authors.lastname: Kassab
authors.forename: Nora M
authors.initials: NM
authors.lastname: Forones
mesh_terms: D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics* / Q000382:microbiology
chemical_list: D016209:Interleukin-8