title: Survey of Anaplasmataceae bacteria in sheep from Senegal.
abstract: OBJECTIVE
  The authors studied the role of bacteria belonging to Anaplasmataceae family as the causes of acute illnesses of sheep in West Africa.
  
  
  METHODS
  We examined and sampled 120 febrile sheep in two regions of Senegal for this study. The DNA extracted from these blood samples was tested by PCR using two pairs of primers (groEL-based and 16S rRNA gene-based).
  
  
  RESULTS
  In 52/120 samples, the microscopic examination revealed intraerythrocytic and/or intraphagocytic spherical inclusions. In 48/52 cases, we succeeded in identifying the bacterial agent: in 38 cases, it was Anaplasma ovis; in six cases, it was Ehrlichia ruminantium; in two cases, Anaplasma phagocytophilum; in one case, Anaplasma platys; and in one case, a yet uncultured Anaplasma sp. closely related to A. phagocytophilum.
  
  
  CONCLUSIONS
  Our studies demonstrated the great variety of pathogenic bacteria from the Anaplasmataceae family in the blood of clinically ill sheep. A. ovis was identified unexpectedly often. For the first time, A. phagocytophilum was found in sub-Saharan Africa, and its further epidemiology may be now reconsidered. The roles of canine pathogen, A. platys, and yet undescribed Anaplasma sp. "Badiouré" in ovine pathology should be more closely studied.
authors.affiliation: Department of Microbiology, National Laboratory for Research on Animal Diseases, Hann, Dakar, BP2057, Senegal.
authors.forename: Mamadou Lamine
authors.initials: ML
authors.lastname: Djiba
authors.forename: Oleg
authors.initials: O
authors.lastname: Mediannikov
authors.forename: Mbaye
authors.initials: M
authors.lastname: Mbengue
authors.forename: Yaya
authors.initials: Y
authors.lastname: Thiongane
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Molez
authors.forename: Momar Talla
authors.initials: MT
authors.lastname: Seck
authors.forename: Florence
authors.initials: F
authors.lastname: Fenollar
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.forename: Mady
authors.initials: M
authors.lastname: Ndiaye
mesh_terms: D000710:Anaplasmataceae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000711:Anaplasmataceae Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012675:Senegal / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
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