title: Inorganic coatings for optimized non-viral transfection of stem cells.
abstract: "Biomimetic" approaches for heterogeneous growth of inorganic coatings have become particularly widespread in biomedical applications, where calcium phosphate (CaP) mineral coatings are used to improve biomedical implants. Changes in coating properties can influence the effects of mineral coatings on adjacent cells, but to date it has not been practical to systematically vary inorganic coating properties to optimize specific cell behaviors. Here, we present an approach to grow CaP mineral coatings in an enhanced throughput format to identify unprecedented capabilities in non-viral gene delivery. Subtle changes in coating properties resulted in widely variable transfection, and optimized coatings led to greater than 10-fold increases in transgene expression by multiple target cell types when compared to standard techniques. The enhanced transfection observed here is substrate-mediated, and related to the characteristics of the local environment near the surface of dissolving mineral coatings. These findings may be particularly translatable to medical device applications.
authors.affiliation: Materials Science Program, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Siyoung
authors.initials: S
authors.lastname: Choi
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yu
authors.forename: Leenaporn
authors.initials: L
authors.lastname: Jongpaiboonkit
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Hollister
authors.forename: William L
authors.initials: WL
authors.lastname: Murphy
mesh_terms: D000818:Animals; D032701:Biomimetics; D002130:Calcium Phosphates; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D004247:DNA / Q000378:metabolism*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D013234:Stem Cells / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D004247:DNA; C020243:calcium phosphate
references.citation: Biomaterials. 1998 Jul;19(14):1245-53
references.pmid: 9720888
references.citation: Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1517-22
references.pmid: 9465047
references.citation: Science. 1999 Oct 29;286(5441):945-7
references.pmid: 10542146
references.citation: Langmuir. 2010 May 18;26(10):7380-7
references.pmid: 20039599
references.citation: Curr Biol. 1998 Dec 3;8(24):1335-8
references.pmid: 9843688
references.citation: Biomaterials. 2002 May;23(10):2211-20
references.pmid: 11962662
references.citation: Acta Biomater. 2006 Mar;2(2):181-9
references.pmid: 16701876
references.citation: Bioconjug Chem. 2002 May-Jun;13(3):621-9
references.pmid: 12009954
references.citation: Arthroscopy. 2009 Dec;25(12):1427-34.e1
references.pmid: 19962070
references.citation: Nature. 1974 Jan 4;247(5435):64-5
references.pmid: 4462607
references.citation: J Biomed Mater Res. 2002 Mar 5;59(3):535-46
references.pmid: 11774312
references.citation: Biomaterials. 2004 Oct;25(22):5323-31
references.pmid: 15110483
references.citation: Biomaterials. 2005 Jan;26(3):327-37
references.pmid: 15262475
references.citation: Nucleic Acids Res. 1996 Feb 15;24(4):596-601
references.pmid: 8604299
references.citation: Nat Mater. 2004 Aug;3(8):569-74
references.pmid: 15258575
references.citation: Pharm Res. 2007 Aug;24(8):1564-71
references.pmid: 17385014
references.citation: Nat Biotechnol. 2008 May;26(5):561-9
references.pmid: 18438401
references.citation: J Biomed Mater Res A. 2003 Nov 1;67(2):578-81
references.pmid: 14566800
references.citation: J Am Chem Soc. 2002 Mar 6;124(9):1910-7
references.pmid: 11866603
references.citation: Biochim Biophys Acta. 1999 Apr 14;1445(1):21-30
references.pmid: 10209255
references.citation: J Biomed Mater Res A. 2006 Apr;77(1):50-8
references.pmid: 16353173
references.citation: Anal Biochem. 2005 Jun 1;341(1):156-64
references.pmid: 15866540
references.citation: J Biol Inorg Chem. 2007 Feb;12(2):174-9
references.pmid: 17031704
references.citation: Int J Nanomedicine. 2011;6:3335-49
references.pmid: 22229000
references.citation: Gene. 2004 Oct 27;341:77-82
references.pmid: 15474290
references.citation: Chemistry. 2004 Jan 23;10(2):463-73
references.pmid: 14735515
references.citation: Biochim Biophys Acta. 2000 Feb 15;1463(2):279-90
references.pmid: 10675506
references.citation: Science. 1994 Apr 1;264(5155):48-55
references.pmid: 17778133
references.citation: Biomaterials. 2011 May;32(14):3611-9
references.pmid: 21334062
references.citation: Biomaterials. 2005 Jan;26(3):285-95
references.pmid: 15262470
references.citation: Biomaterials. 2009 Oct;30(31):6386-93
references.pmid: 19695695
references.citation: Biotechnol J. 2012 Jan;7(1):90-102
references.pmid: 22147583
references.citation: J Am Chem Soc. 2001 Aug 22;123(33):8155-6
references.pmid: 11506588
references.citation: J Biomed Mater Res A. 2007 Dec 15;83(4):1076-1086
references.pmid: 17584901
references.citation: Biomaterials. 1997 Sep;18(17):1185-90
references.pmid: 9259516
references.citation: Int J Pharm. 2009 Feb 23;368(1-2):186-92
references.pmid: 18996454
references.citation: Spine J. 2011 Jun;11(6):560-7
references.pmid: 20097616
references.citation: Langmuir. 2012 Feb 28;28(8):3871-80
references.pmid: 22242934
references.citation: Nature. 1973 Jun 15;243(5407):420-1
references.pmid: 4743638