title: Direct transfer of whole genomes from bacteria to yeast.
abstract: Transfer of genomes into yeast facilitates genome engineering for genetically intractable organisms, but this process has been hampered by the need for cumbersome isolation of intact genomes before transfer. Here we demonstrate direct cell-to-cell transfer of bacterial genomes as large as 1.8 megabases (Mb) into yeast under conditions that promote cell fusion. Moreover, we discovered that removal of restriction endonucleases from donor bacteria resulted in the enhancement of genome transfer.
authors.affiliation: Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, San Diego, California, USA. bkaras@jcvi.org
authors.forename: Bogumil J
authors.initials: BJ
authors.lastname: Karas
authors.forename: Jelena
authors.initials: J
authors.lastname: Jablanovic
authors.forename: Lijie
authors.initials: L
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Goldgof
authors.forename: Jason
authors.initials: J
authors.lastname: Stam
authors.forename: Adi
authors.initials: A
authors.lastname: Ramon
authors.forename: Micah J
authors.initials: MJ
authors.lastname: Manary
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Winzeler
authors.forename: J Craig
authors.initials: JC
authors.lastname: Venter
authors.forename: Philip D
authors.initials: PD
authors.lastname: Weyman
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Gibson
authors.forename: John I
authors.initials: JI
authors.lastname: Glass
authors.forename: Clyde A
authors.initials: CA
authors.lastname: Hutchison
authors.forename: Hamilton O
authors.initials: HO
authors.lastname: Smith
authors.forename: Yo
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D016680:Genome, Bacterial; D016681:Genome, Fungal; D014162:Transfection
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