title: New stilbenoids isolated from fungus-challenged black skin peanut seeds and their adipogenesis inhibitory activity in 3T3-L1 cells.
abstract: One new stilbene derivative (3,5,3'-trihydroxy-4'-methoxy-5'-isopentenylstilbene, MIP) and two new stilbene dimers (arahypin-11 and arahypin-12) together with three known stilbenoids (arachidin-1, arachidin-3, and SB-1) were isolated from black skin peanut seeds challenged by the fungal strain Rhizopus oligoporus . The structures of the three new compounds were elucidated by analysis of HRESIMS, UV, 1D and 2D NMR spectra. The antiadipogenic and cytotoxic effects of the isolated compounds were investigated using 3T3-L1 cells at a concentration range of 1-10 μM. Among the compounds tested, arachidin-1 inhibited the 3T3-L1 adipocyte differentiation dose-dependently, whereas arahypin-11 and arahypin-12 exhibited significant cytotoxicity in 3T3-L1 preadipocytes.
authors.affiliation: Food Science and Technology Programme, ‡Department of Chemistry, National University of Singapore , 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Liu
authors.forename: Ji'en
authors.initials: J
authors.lastname: Wu
authors.forename: Dejian
authors.initials: D
authors.lastname: Huang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D010367:Arachis / Q000737:chemistry* / Q000382:microbiology; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry; D005658:Fungi; D045782:Hemiterpenes / Q000737:chemistry; D051379:Mice; D012639:Seeds / Q000737:chemistry* / Q000382:microbiology; D013267:Stilbenes / Q000737:chemistry*; D013312:Stress, Physiological
chemical_list: D005419:Flavonoids; D045782:Hemiterpenes; D013267:Stilbenes; C520888:arachidin-1; C553171:arachidin-3; C047101:ganhuangenin