title: Assessment of nanosilver toxicity during zebrafish (Danio rerio) development.
abstract: Nanomaterials (NMs) including silver nanoparticles (AgNPs) are incorporated into an increasing number of consumer and medical products. However, the potential toxicity of AgNPs to aquatic organisms is largely unknown. This study characterizes the effects of AgNPs on zebrafish (Danio rerio) development. The effects of silver ions (Ag(+)) and AgNPs were examined at equivalent Ag concentrations, which ranged from 0.03 to 1.55 μg mL(-1) total Ag. The Ag(+) was more toxic than AgNPs but both lead to death and delayed hatching in surviving embryos. Both silver types depleted glutathione levels but generally did not affect antioxidant enzymes activities. In addition to silver some of the embryos were also exposed to cysteine, which generally reduced the toxicity of both silver types. This study demonstrates that AgNPs and Ag(+) are capable of inducing toxicity in zebrafish embryos including the induction of oxidative stress.
authors.affiliation: Department of Biology & Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada. amass017@uottawa.ca
authors.forename: Andrey
authors.initials: A
authors.lastname: Massarsky
authors.forename: Lisa
authors.initials: L
authors.lastname: Dupuis
authors.forename: Jessica
authors.initials: J
authors.lastname: Taylor
authors.forename: Shahram
authors.initials: S
authors.lastname: Eisa-Beygi
authors.forename: Laura
authors.initials: L
authors.lastname: Strek
authors.forename: Vance L
authors.initials: VL
authors.lastname: Trudeau
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Moon
mesh_terms: D000818:Animals; D003545:Cysteine / Q000737:chemistry; D047108:Embryonic Development / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006339:Heart Rate / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry; D012835:Silver Nitrate / Q000737:chemistry / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development*
chemical_list: D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D012834:Silver; D012835:Silver Nitrate; D005978:Glutathione; D003545:Cysteine