title: [The second generation universal oligonucleotide microarray for subtyping of influenza virus A].
abstract: The microchip for influenza A subtyping was developed, functioning on a principle "one spot--one subtype". Each spot contains the set of oligonucleotide probes, specific for a particular subtype of hemagglutinin, neuraminidase or matrix gene. Reliability of the proposed chip version is the same as for earlier created in our group full-size microchip for separate hemagglutinin and neuraminidase subtyping. To visualize the image, analyzed DNA can be labeled by either fluorescent dye or biotin with the further fixation in system streptavidin-gold nanoparticles and image development by silver precipitation. In the second case common version of scanner can be used for the image analysis, that essentially simplifies procedure of influenza A subtyping.
authors.forename: E V
authors.initials: EV
authors.lastname: Kostina
authors.forename: V A
authors.initials: VA
authors.lastname: Riabinin
authors.forename: G A
authors.initials: GA
authors.lastname: Maksakova
authors.forename: A N
authors.initials: AN
authors.lastname: Siniakov
mesh_terms: D060005:Genotyping Techniques; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods; D009439:Neuraminidase / Q000235:genetics / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D015345:Oligonucleotide Probes; D009439:Neuraminidase