title: Altering colloidal surface functionalization using DNA encapsulated inside monodisperse gelatin microsphere templates.
abstract: Soluble oligonucleotides are typically introduced to bulk solution to promote hybridization activity on DNA-functionalized surfaces. Here, an alternative approach is explored by encapsulating secondary target strands inside semipermeable colloidal satellite assemblies, then triggering their release at 37 °C for subsequent surface hybridization activity. To prepare DNA-loaded satellite assemblies, uniform gelatin microspheres are fabricated using microfluidics, loaded with 15 base-long secondary DNA targets, capped with a polyelectrolyte bilayer, and finally coated with a monolayer of polystyrene microspheres functionalized with duplexes comprised of immobilized probes and soluble, 13 base-long hybridization partner strands. Once warmed to 37 °C, secondary DNA targets are released from the gelatin template and then competitively displace the shorter, original hybridization partners on the polystyrene microspheres.
authors.affiliation: School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia 30032-0245, United States.
authors.forename: James O
authors.initials: JO
authors.lastname: Hardin
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernandez-Nieves
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Martinez
authors.forename: Valeria T
authors.initials: VT
authors.lastname: Milam
mesh_terms: D002214:Capsules / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D008863:Microspheres; D010316:Particle Size; D013499:Surface Properties
chemical_list: D002214:Capsules; D003102:Colloids; D005780:Gelatin; D004247:DNA