title: High environmental temperature increases glucose requirement in the developing chicken embryo.
abstract: Environmental conditions during the perinatal period influence metabolic and developmental processes in mammals and avian species, which could impact pre- and postnatal survival and development. The current study investigated the effect of eggshell temperature (EST) on glucose metabolism in broiler chicken embryos. Broiler eggs were incubated at a high (38.9°C) or normal (37.8°C) EST from day 10.5 of incubation onward and were injected with a bolus of [U-(13)C]glucose in the chorio-allantoic fluid at day 17.5 of incubation. After [U-(13)C]glucose administration, (13)C enrichment was determined in intermediate pools and end-products of glucose metabolism. Oxidation of labeled glucose occurred for approximately 3 days after injection. Glucose oxidation was higher in the high than in the normal EST treatment from day 17.6 until 17.8 of incubation. The overall recovery of (13)CO2 tended to be 4.7% higher in the high than in the normal EST treatment. An increase in EST (38.9°C vs 37.8°C) increased (13)C enrichment in plasma lactate at day 17.8 of incubation and (13)C in hepatic glycogen at day 18.8 of incubation. Furthermore, high compared to normal EST resulted in a lower yolk-free body mass at day 20.9 (-2.74 g) and 21.7 (-3.81 g) of incubation, a lower hepatic glycogen concentration at day 18.2 (-4.37 mg/g) and 18.8 (-4.59 mg/g) of incubation, and a higher plasma uric acid concentration (+2.8 mg/mL/+43%) at day 21.6 of incubation. These results indicate that the glucose oxidation pattern is relatively slow, but the intensity increased consistently with an increase in developmental stage of the embryo. High environmental temperatures in the perinatal period of chicken embryos increased glucose oxidation and decreased hepatic glycogen prior to the hatching process. This may limit glucose availability for successful hatching and could impact body development, probably by increased gluconeogenesis from glucogenic amino acids to allow anaerobic glycolysis.
authors.affiliation: HatchTech B.V., Veenendaal, The Netherlands. mail@roosmolenaar.com
authors.forename: Roos
authors.initials: R
authors.lastname: Molenaar
authors.forename: Joost J G C
authors.initials: JJ
authors.lastname: van den Borne
authors.forename: Ewoud
authors.initials: E
authors.lastname: Hazejager
authors.forename: Niels B
authors.initials: NB
authors.lastname: Kristensen
authors.forename: Marcel J W
authors.initials: MJ
authors.lastname: Heetkamp
authors.forename: Ron
authors.initials: R
authors.lastname: Meijerhof
authors.forename: Bas
authors.initials: B
authors.lastname: Kemp
authors.forename: Henry
authors.initials: H
authors.lastname: van den Brand
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002247:Carbon Isotopes; D002642:Chick Embryo; D002645:Chickens / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D004777:Environment; D005947:Glucose / Q000378:metabolism*; D006358:Hot Temperature; D019344:Lactic Acid / Q000097:blood; D008112:Liver Glycogen / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology*; D014527:Uric Acid / Q000097:blood
chemical_list: D002247:Carbon Isotopes; D008112:Liver Glycogen; D002245:Carbon Dioxide; D014527:Uric Acid; D019344:Lactic Acid; D005947:Glucose; D010100:Oxygen
references.citation: J Pediatr Gastroenterol Nutr. 2002 Feb;34(2):158-64
references.pmid: 11840033
references.citation: Anal Chem. 1995 Mar 1;67(5):858-62
references.pmid: 7762822
references.citation: Respir Physiol. 1983 Aug;53(2):173-85
references.pmid: 6415775
references.citation: Comp Biochem Physiol. 1965 Feb;14:217-22
references.pmid: 14325998
references.citation: Poult Sci. 2007 May;86(5):1043-9
references.pmid: 17435045
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Nov 1;877(29):3638-44
references.pmid: 19775945
references.citation: J Embryol Exp Morphol. 1965 Dec;14(3):273-80
references.pmid: 5861723
references.citation: Reprod Suppl. 2001;58:247-61
references.pmid: 11980194
references.citation: Poult Sci. 2005 Jun;84(6):914-20
references.pmid: 15971530
references.citation: Poult Sci. 2011 Jun;90(6):1302-12
references.pmid: 21597072
references.citation: Comp Biochem Physiol A Comp Physiol. 1975 Aug 1;51(4):769-76
references.pmid: 237698
references.citation: Poult Sci. 2007 Sep;86(9):1960-71
references.pmid: 17704385
references.citation: Enzymologia. 1962 May 15;24:230-6
references.pmid: 14492328
references.citation: Biochim Biophys Acta. 1963 Jun 4;71:578-88
references.pmid: 13966157
references.citation: Poult Sci. 2006 Apr;85(4):770-6
references.pmid: 16615362
references.citation: J Nutr. 2011 Sep;141(9):1657-63
references.pmid: 21795428
references.citation: Arch Biochem Biophys. 1966 Jun;114(3):543-6
references.pmid: 4289267
references.citation: Cytobios. 1987;49(198-199):197-210
references.pmid: 2953561
references.citation: Br J Nutr. 2002 Jun;87(6):569-77
references.pmid: 12067427
references.citation: Comp Biochem Physiol B. 1986;85(4):727-31
references.pmid: 3816147
references.citation: Poult Sci. 1988 Mar;67(3):463-9
references.pmid: 2970065
references.citation: Poult Sci. 2010 Sep;89(9):2010-21
references.pmid: 20709988
references.citation: Am J Physiol. 1959 Jul;197(1):47-51
references.pmid: 13661386
references.citation: Poult Sci. 2010 Dec;89(12):2678-90
references.pmid: 21076107
references.citation: Poult Sci. 2007 Dec;86(12):2685-91
references.pmid: 18029817
references.citation: Poult Sci. 2011 Mar;90(3):624-32
references.pmid: 21325234
references.citation: Z Ernahrungswiss. 1997 Dec;36(4):268-72
references.pmid: 9467214
references.citation: Exp Physiol. 2007 Mar;92(2):287-98
references.pmid: 17170060
references.citation: J Nutr. 2007 Oct;137(10):2234-41
references.pmid: 17885004
references.citation: Biosci Biotechnol Biochem. 2002 Feb;66(2):426-9
references.pmid: 11999421
references.citation: Comp Biochem Physiol C Comp Pharmacol. 1981;69C(1):1-6
references.pmid: 6113077
references.citation: Poult Sci. 2007 Oct;86(10):2194-9
references.pmid: 17878449
references.citation: Proc Nutr Soc. 1971 Dec;30(3):254-9
references.pmid: 4949518
references.citation: Poult Sci. 2011 Jun;90(6):1257-66
references.pmid: 21597067
references.citation: Am J Clin Nutr. 2000 Aug;72(2):432-8
references.pmid: 10919938
references.citation: J Anim Sci. 2010 Mar;88(3):912-21
references.pmid: 19966165
references.citation: Comp Biochem Physiol. 1969 Mar;28(3):1169-76
references.pmid: 5786822
references.citation: Poult Sci. 2001 Dec;80(12):1729-35
references.pmid: 11771888
references.citation: Proc Soc Exp Biol Med. 1960 Jul;104:515-8
references.pmid: 14409078
references.citation: Poult Sci. 2012 Feb;91(2):444-53
references.pmid: 22252359