title: Bone marrow-derived mononuclear cells vs. mesenchymal stromal cells in experimental allergic asthma.
abstract: We compared the effects of bone marrow-derived mononuclear cells (BMMCs) and mesenchymal stromal cells (MSCs) on airway inflammation and remodeling and lung mechanics in experimental allergic asthma. C57BL/6 mice were sensitized and challenged with ovalbumin (OVA group). A control group received saline using the same protocol. Twenty-four hours after the last challenge, groups were further randomized into subgroups to receive saline, BMMCs (2×10(6)) or MSCs (1×10(5)) intratracheally. BMMC and MSC administration decreased cell infiltration, bronchoconstriction index, alveolar collapse, collagen fiber content in the alveolar septa, and interleukin (IL)-4, IL-13, transforming growth factor (TGF)-β and vascular endothelial growth factor (VEGF) levels compared to OVA-SAL. Lung function, alveolar collapse, collagen fiber deposition in alveolar septa, and levels of TGF-β and VEGF improved more after BMMC than MSC therapy. In conclusion, intratracheal BMMC and MSC administration effectively modulated inflammation and fibrogenesis in an experimental model of asthma, but BMMCs was associated with greater benefit in terms of reducing levels of fibrogenesis-related growth factors.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Soraia C
authors.initials: SC
authors.lastname: Abreu
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Antunes
authors.forename: Júlia C
authors.initials: JC
authors.lastname: de Castro
authors.forename: Milena V
authors.initials: MV
authors.lastname: de Oliveira
authors.forename: Elga
authors.initials: E
authors.lastname: Bandeira
authors.forename: Debora S
authors.initials: DS
authors.lastname: Ornellas
authors.forename: Bruno L
authors.initials: BL
authors.lastname: Diaz
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
authors.forename: Débora G
authors.initials: DG
authors.lastname: Xisto
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001249:Asthma / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D001854:Bone Marrow Cells / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D010047:Ovalbumin