title: Phenotypic and Genotypic Characterization of Escherichia coli Isolated from Untreated Surface Waters.
abstract: A common member of the intestinal microbiota in humans and animals is Escherichia coli. Based on the presence of virulence factors, E. coli can be potentially pathogenic. The focus of this study was to isolate E. coli from untreated surface waters (37 sites) in Illinois and Missouri and determine phenotypic and genotypic diversity among isolates. Water samples positive for fecal coliforms based on the Colisure(®) test were streaked directly onto Eosin Methylene Blue (EMB) agar (37°C) or transferred to EC broth (44.5°C). EC broth cultures producing gas were then streaked onto EMB agar. Forty-five isolates were identified as E. coli using API 20E and Enterotube II identification systems, and some phenotypic variation was observed in metabolism and fermentation. Antibiotic susceptibility of each isolate was also determined using the Kirby-Bauer Method. Differential responses to 10 antimicrobial agents were seen with 7, 16, 2, and 9 of the isolates resistant to ampicillin, cephalothin, tetracycline, and triple sulfonamide, respectively. All of the isolates were susceptible or intermediate to amoxicillin, ciprofloxacin, polymyxin B, gentamicin, imipenem, and nalidixic acid. Genotypic variation was assessed through multiplex Polymerase Chain Reaction for four virulence genes (stx1 and stx2 [shiga toxin], eaeA [intimin]; and hlyA [enterohemolysin]) and one housekeeping gene (uidA [β-D-glucuronidase]). Genotypic variation was observed with two of the isolates possessing the virulence gene (eaeA) for intimin. These findings increase our understanding of the diversity of E. coli in the environment which will ultimately help in the assessment of this organism and its role in public health.
authors.affiliation: Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois 61920, USA.
authors.forename: Kristopher J
authors.initials: KJ
authors.lastname: Janezic
authors.forename: Blake
authors.initials: B
authors.lastname: Ferry
authors.forename: Eric W
authors.initials: EW
authors.lastname: Hendricks
authors.forename: Brian A
authors.initials: BA
authors.lastname: Janiga
authors.forename: Tiffany
authors.initials: T
authors.lastname: Johnson
authors.forename: Samantha
authors.initials: S
authors.lastname: Murphy
authors.forename: Morgan E
authors.initials: ME
authors.lastname: Roberts
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Scott
authors.forename: Alexandra N
authors.initials: AN
authors.lastname: Theisen
authors.forename: Kai F
authors.initials: KF
authors.lastname: Hung
authors.forename: Steven L
authors.initials: SL
authors.lastname: Daniel
keywords: E. coli; antibiotics; intimin; multiplex PCR; surface water; β-D-glucuronidase.
references.citation: Appl Environ Microbiol. 2005 Aug;71(8):4461-8
references.pmid: 16085837
references.citation: Appl Environ Microbiol. 2011 Dec;77(23):8427-33
references.pmid: 21965413
references.citation: J Biomed Biotechnol. 2012;2012:834598
references.pmid: 22791963
references.citation: PLoS One. 2011;6(6):e20819
references.pmid: 21687635
references.citation: BMC Microbiol. 2009 Mar 05;9:50
references.pmid: 19265535
references.citation: Nat Rev Microbiol. 2010 Jan;8(1):26-38
references.pmid: 19966814
references.citation: Appl Environ Microbiol. 2012 Oct;78(20):7317-26
references.pmid: 22885746
references.citation: FEMS Microbiol Ecol. 2003 Jun 1;44(3):303-8
references.pmid: 19719611
references.citation: Water Res. 2011 Dec 1;45(19):6321-33
references.pmid: 21982678
references.citation: J Clin Microbiol. 1998 Feb;36(2):598-602
references.pmid: 9466788
references.citation: Int J Antimicrob Agents. 2011 Apr;37(4):365-7
references.pmid: 21330111
references.citation: J Water Health. 2008 Jun;6(2):197-207
references.pmid: 18209282
references.citation: Appl Environ Microbiol. 1981 Mar;41(3):657-63
references.pmid: 7013706
references.citation: PLoS One. 2012;7(10):e47005
references.pmid: 23056556
references.citation: Genome Biol. 2007;8(7):R138
references.pmid: 17711596
references.citation: Nat Rev Microbiol. 2004 Feb;2(2):123-40
references.pmid: 15040260
references.citation: Environ Microbiol. 2006 Mar;8(3):504-13
references.pmid: 16478456
references.citation: MMWR Morb Mortal Wkly Rep. 2011 Sep 9;60(35):1197-202
references.pmid: 21900873
references.citation: Vet Microbiol. 2008 Nov 25;132(1-2):1-18
references.pmid: 18586416
references.citation: Appl Environ Microbiol. 2012 Dec;78(23):8272-80
references.pmid: 23001658
references.citation: Appl Environ Microbiol. 2007 Jun;73(12):3771-8
references.pmid: 17468280
references.citation: Appl Environ Microbiol. 1997 Jul;63(7):2607-12
references.pmid: 9212410
references.citation: J Appl Microbiol. 2007 May;102(5):1372-81
references.pmid: 17448172
references.citation: PLoS One. 2010 Jan 14;5(1):e8700
references.pmid: 20090843
references.citation: Environ Pollut. 2009 Nov;157(11):2893-902
references.pmid: 19560847
references.citation: Emerg Infect Dis. 2005 Apr;11(4):603-9
references.pmid: 15829201
references.citation: J Clin Microbiol. 1996 Jul;34(7):1788-93
references.pmid: 8784591
references.citation: Appl Environ Microbiol. 2006 Jan;72(1):612-21
references.pmid: 16391098
references.citation: J Biol Dyn. 2012;6:1052-66
references.pmid: 22988977
references.citation: Appl Environ Microbiol. 1991 Apr;57(4):1013-7
references.pmid: 2059028
references.citation: Infect Ecol Epidemiol. 2012;2:
references.pmid: 22957135
references.citation: FEMS Microbiol Ecol. 2012 Jul;81(1):267-80
references.pmid: 22486636
references.citation: J Water Health. 2012 Mar;10(1):1-10
references.pmid: 22361697
references.citation: Can J Microbiol. 1990 Dec;36(12):891-4
references.pmid: 2081335
references.citation: Appl Environ Microbiol. 2005 Jun;71(6):3041-8
references.pmid: 15933000
references.citation: Environ Sci Technol. 2002 Dec 15;36(24):5279-88
references.pmid: 12521151
references.citation: Water Res. 2006 Feb;40(3):401-26
references.pmid: 16434075
references.citation: Appl Environ Microbiol. 2011 Jun;77(12):3988-97
references.pmid: 21515724
references.citation: Nat Rev Microbiol. 2010 Mar;8(3):207-17
references.pmid: 20157339
references.citation: Biotechnol Adv. 2012 Sep-Oct;30(5):1001-10
references.pmid: 21907272
references.citation: J Water Health. 2004 Jun;2(2):103-14
references.pmid: 15387134
references.citation: World J Gastroenterol. 2011 Apr 14;17(14):1910-4
references.pmid: 21528067
references.citation: J Clin Microbiol. 2003 May;41(5):2113-25
references.pmid: 12734257
references.citation: J Clin Microbiol. 1994 Mar;32(3):823-4
references.pmid: 8195398
references.citation: Eur J Clin Microbiol Infect Dis. 2012 Jun;31(6):1081-7
references.pmid: 21938538
references.citation: MMWR Surveill Summ. 2011 Sep 23;60(12):38-68
references.pmid: 21937977
references.citation: Microbes Environ. 2008;23(2):101-8
references.pmid: 21558695
references.citation: MMWR Surveill Summ. 2011 Sep 23;60(12):1-32
references.pmid: 21937976