title: The potential for transmission of BCG from orally vaccinated white-tailed deer (Odocoileus virginianus) to cattle (Bos taurus) through a contaminated environment: experimental findings.
abstract: White-tailed deer (Odocoileus virginianus) experimentally infected with a virulent strain of Mycobacterium bovis have been shown to transmit the bacterium to other deer and cattle (Bos taurus) by sharing of pen waste and feed. The risk of transmission of M. bovis bacille Calmette-Guerin (BCG) vaccine from orally vaccinated white-tailed deer to other deer and cattle, however, is not well understood. In order to evaluate this risk, we orally vaccinated 14 white-tailed deer with 1×10(9) colony forming units BCG in lipid-formulated baits and housed them with nine non-vaccinated deer. Each day we exposed the same seven naïve cattle to pen space utilized by the deer to look for transmission between the two species. Before vaccination and every 60 days until the end of the study, we performed tuberculin skin testing on deer and cattle, as well as interferon-gamma testing in cattle, to detect cellular immune response to BCG exposure. At approximately 27 weeks all cattle and deer were euthanized and necropsied. None of the cattle converted on either caudal fold, comparative cervical tests, or interferon-gamma assay. None of the cattle were culture positive for BCG. Although there was immunological evidence that BCG transmission occurred from deer to deer, we were unable to detect immunological or microbiological evidence of transmission to cattle. This study suggests that the risk is likely to be low that BCG-vaccinated white-tailed deer would cause domestic cattle to react to the tuberculin skin test or interferon-gamma test through exposure to a BCG-contaminated environment.
authors.affiliation: United States Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Wildlife Research Center, Fort Collins, Colorado, United States of America. pauline.nol@aphis.usda.gov
authors.forename: Pauline
authors.initials: P
authors.lastname: Nol
authors.forename: Jack C
authors.initials: JC
authors.lastname: Rhyan
authors.forename: Suelee
authors.initials: S
authors.lastname: Robbe-Austerman
authors.forename: Matt P
authors.initials: MP
authors.lastname: McCollum
authors.forename: Tara D
authors.initials: TD
authors.lastname: Rigg
authors.forename: Nadia T
authors.initials: NT
authors.lastname: Saklou
authors.forename: Mo D
authors.initials: MD
authors.lastname: Salman
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology*; D002417:Cattle; D003670:Deer; D009163:Mycobacterium bovis / Q000276:immunology*; D012882:Skin Tests; D014376:Tuberculosis / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000175:diagnosis / Q000517:prevention & control* / Q000635:transmission*
chemical_list: D001500:BCG Vaccine
references.citation: Emerg Infect Dis. 2008 Aug;14(8):1330-2
references.pmid: 18680678
references.citation: J Wildl Dis. 1997 Oct;33(4):749-58
references.pmid: 9391958
references.citation: Vet Microbiol. 2006 Feb 25;112(2-4):313-23
references.pmid: 16376030
references.citation: J Wildl Dis. 2008 Apr;44(2):247-59
references.pmid: 18436658
references.citation: Zoonoses Public Health. 2009 Jun;56(5):243-51
references.pmid: 19175569
references.citation: Vaccine. 2007 Sep 4;25(36):6589-97
references.pmid: 17688976
references.citation: Am J Vet Res. 2001 May;62(5):692-6
references.pmid: 11341387
references.citation: Am J Vet Res. 2004 Nov;65(11):1483-9
references.pmid: 15566085
references.citation: Zoonoses Public Health. 2010 Dec;57(7-8):e206-12
references.pmid: 20707863
references.citation: Vaccine. 2005 May 20;23(27):3581-9
references.pmid: 15855017
references.citation: Jpn J Infect Dis. 2011;64(3):234-6
references.pmid: 21617309
references.citation: Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):486-90
references.pmid: 18218954
references.citation: Clin Vaccine Immunol. 2006 Jun;13(6):611-9
references.pmid: 16760317
references.citation: Rev Sci Tech. 2002 Aug;21(2):317-34
references.pmid: 11974618
references.citation: Tuberculosis (Edinb). 2008 Nov;88(6):595-600
references.pmid: 18439875
references.citation: J Wildl Dis. 2004 Jan;40(1):87-91
references.pmid: 15137492