title: Structure of an atypical FeoB G-domain reveals a putative domain-swapped dimer.
abstract: FeoB is a transmembrane protein involved in ferrous iron uptake in prokaryotic organisms. FeoB comprises a cytoplasmic soluble domain termed NFeoB and a C-terminal polytopic transmembrane domain. Recent structures of NFeoB have revealed two structural subdomains: a canonical GTPase domain and a five-helix helical domain. The GTPase domain hydrolyses GTP to GDP through a well characterized mechanism, a process which is required for Fe(2+) transport. In contrast, the precise role of the helical domain has not yet been fully determined. Here, the structure of the cytoplasmic domain of FeoB from Gallionella capsiferriformans is reported. Unlike recent structures of NFeoB, the G. capsiferriformans NFeoB structure is highly unusual in that it does not contain a helical domain. The crystal structures of both apo and GDP-bound protein forms a domain-swapped dimer.
authors.affiliation: Structural Biology Program, Centenary Institute, Sydney, NSW 2042, Australia.
authors.forename: Chandrika N
authors.initials: CN
authors.lastname: Deshpande
authors.forename: Aaron P
authors.initials: AP
authors.lastname: McGrath
authors.forename: Josep
authors.initials: J
authors.lastname: Font
authors.forename: Amy P
authors.initials: AP
authors.lastname: Guilfoyle
authors.forename: Megan J
authors.initials: MJ
authors.lastname: Maher
authors.forename: Mika
authors.initials: M
authors.lastname: Jormakka
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D020558:GTP Phosphohydrolases / Q000737:chemistry*; D043372:Gallionellaceae / Q000201:enzymology*; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D040681:Structural Homology, Protein
chemical_list: D008565:Membrane Proteins; D020558:GTP Phosphohydrolases
keywords: FeoB; Gallionella capsiferriformans; NFeoB; domain-swapped dimer
references.citation: J Mol Biol. 2005 Sep 9;352(1):202-11
references.pmid: 16061250
references.citation: Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
references.pmid: 21460454
references.citation: Annu Rev Biochem. 2011;80:943-71
references.pmid: 21675921
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
references.pmid: 20057044
references.citation: Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):61-9
references.pmid: 18094468
references.citation: Methods Mol Biol. 2008;426:419-35
references.pmid: 18542881
references.citation: J Mol Biol. 2007 Sep 21;372(3):774-97
references.pmid: 17681537
references.citation: EMBO J. 2009 Sep 2;28(17):2677-85
references.pmid: 19629046
references.citation: J Struct Biol. 2010 Jun;170(3):501-12
references.pmid: 20123128
references.citation: FEBS Lett. 2010 Feb 19;584(4):733-8
references.pmid: 20036663
references.citation: J Mol Biol. 2008 Jan 25;375(4):1086-97
references.pmid: 18068722
references.citation: Annu Rev Microbiol. 2010;64:561-83
references.pmid: 20565252
references.citation: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1511-5
references.pmid: 22139154
references.citation: Bioorg Med Chem. 2005 Sep 1;13(17):5013-20
references.pmid: 15993087
references.citation: J Biol Chem. 2010 May 7;285(19):14594-602
references.pmid: 20220129
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):48-57
references.pmid: 16369093
references.citation: Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
references.pmid: 15572765
references.citation: Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):32-41
references.pmid: 17164524
references.citation: Structure. 2009 Oct 14;17(10):1345-55
references.pmid: 19733088
references.citation: Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):973-80
references.pmid: 22101824
references.citation: J Bacteriol. 1993 Oct;175(19):6212-9
references.pmid: 8407793
references.citation: Biometals. 2006 Apr;19(2):143-57
references.pmid: 16718600
references.citation: J Bacteriol. 2012 Dec;194(23):6518-26
references.pmid: 23024345
references.citation: Protein Sci. 2011 Feb;20(2):448-56
references.pmid: 21280135
references.citation: FEBS Lett. 2012 Jul 30;586(16):2218-24
references.pmid: 22750478
references.citation: Protein Sci. 2001 Nov;10(11):2325-35
references.pmid: 11604538