title: Dietary glucosylceramide enhances tight junction function in skin epidermis via induction of claudin-1.
abstract: Dietary glucosylceramide increased the expression of claudin-1 in UVB-irradiated mouse epidermis. Sphingosine and phytosphingosine, metabolites of glucosylceramide, increased trans-epithelial electrical resistance, and phytosphingosine increased claudin-1 mRNA expression in cultured keratinocytes. Our results indicate that the skin barrier improvement induced by dietary glucosylceramide might be due to enhancement of tight junction function, mediated by increased expression of claudin-1 induced by sphingoid metabolites.
authors.affiliation: Tokyo University of Agriculture and Technology, Faculty of Agriculture, Scleroprotein and Leather Research Institute, Tokyo, Japan.
authors.forename: Chinatsu
authors.initials: C
authors.lastname: Kawada
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Mutsuto
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nomura
mesh_terms: D000818:Animals; D002460:Cell Line; D062445:Claudin-1 / Q000235:genetics*; D004040:Dietary Carbohydrates / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D005963:Glucosylceramides / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D021381:Protein Transport / Q000187:drug effects / Q000528:radiation effects; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D015533:Transcriptional Activation / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D062445:Claudin-1; D004040:Dietary Carbohydrates; D005963:Glucosylceramides