title: Effect of enamel matrix derivative on bone formation around intraosseous titanium implant: An experimental study in canine model.
abstract: BACKGROUND
  The purpose of this study was to perform a histological, histomorphometrical, and immunohistochemical evaluation of the effect of Enamel matrix derivative (EMD) on bone formation around titanium dental implant.
  
  
  METHODS
  In this animal study, 12 implants (10 × 3.8 mm) were inserted in the tibia bone of three dogs of Iranian breed. Two implants were placed in each tibia with EMD only on the left side. The dogs were sacrificed 2, 4, and 6 weeks after implantation. Following decalcification of the implants' surrounding tissue and preparation of 4 μm thick sections, they were stained with hematoxylin and eosin (H and E) and immunohistochemical (IHC) stain for osteopontin (OPN) marker. Histomorphometric evaluation was performed via measurement of the percentage of the woven, lamellar, and total generated bone. Light microscopy osteoblastic intensity of OPN in osteoblasts and bone matrix was also evaluated Data were analyzed by Wilcoxon signed Ranks, and Mc Nemar tests.
  
  
  RESULTS
  In both control and EMD-applied groups, bone formation was recognized around the implants at the 4(th) week postimplantation. The percentage of total generated bone in the test group was higher than the control group, although being not statistically significant (P value = 0.917). Osteoclasts exhibited significantly higher proliferation activity compared the control group when stimulated by EMD (P value = 0.027). On average, the staining intensity in osteoblasts and extracellular matrix of bone, in EMD-applied subjects was higher than those of the controls (P value = 0.167 and P value = 0.414, respectively).
  
  
  CONCLUSIONS
  EMD enhanced bone formation around dental implants, but this increase was not significant.
authors.affiliation: Dental Implants Research Center, Department of Periodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Birang
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shahabooei
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mashhadiabbas
authors.forename: Mehdi
authors.initials: M
authors.lastname: Atabaki
authors.forename: Narges
authors.initials: N
authors.lastname: Naghsh
authors.forename: Keikavous
authors.initials: K
authors.lastname: Kavosh
authors.forename: Ehsan
authors.initials: E
authors.lastname: Birang
authors.forename: Ahmad
authors.initials: A
authors.lastname: Mogharehabed
keywords: Bone formation; dental implant; enamel matrix protein; osteopontin
references.citation: Crit Rev Oral Biol Med. 2000;11(3):279-303
references.pmid: 11021631
references.citation: J Periodontol. 2000 Aug;71(8):1287-96
references.pmid: 10972644
references.citation: J Clin Periodontol. 1997 Sep;24(9 Pt 2):705-14
references.pmid: 9310876
references.citation: Int J Oral Maxillofac Surg. 2002 Oct;31(5):469-84
references.pmid: 12418561
references.citation: J Biomed Mater Res. 2002 May;60(2):269-76
references.pmid: 11857433
references.citation: Science. 2000 Sep 1;289(5484):1504-8
references.pmid: 10968780
references.citation: Implant Dent. 2008 Jun;17(2):200-7
references.pmid: 18545052
references.citation: J Dent. 2005 Oct;33(9):749-55
references.pmid: 16199283
references.citation: J Periodontol. 2002 May;73(5):543-50
references.pmid: 12027258
references.citation: Eur J Oral Sci. 1998 Jan;106 Suppl 1:282-91
references.pmid: 9541238
references.citation: J Dent Res. 2008 Aug;87(8):782-7
references.pmid: 18650553
references.citation: J Clin Periodontol. 1997 Sep;24(9 Pt 2):693-6
references.pmid: 9310874
references.citation: Bone. 2011 Apr 1;48(4):918-26
references.pmid: 21172465
references.citation: Crit Rev Oral Biol Med. 1995;6(2):84-108
references.pmid: 7548623
references.citation: Ciba Found Symp. 1997;205:246-55; discussion 255-60
references.pmid: 9189629
references.citation: Med Oral Patol Oral Cir Bucal. 2011 Jan 01;16(1):e42-4
references.pmid: 20526267
references.citation: J Clin Periodontol. 2003 Apr;30(4):359-63
references.pmid: 12694436
references.citation: Arch Oral Biol. 1995 Jan;40(1):1-14
references.pmid: 7748107
references.citation: J Periodontol. 2002 Jul;73(7):789-96
references.pmid: 12146539
references.citation: J Periodontal Res. 2006 Oct;41(5):471-6
references.pmid: 16953824
references.citation: J Clin Periodontol. 2001 Feb;28(2):181-8
references.pmid: 11168744
references.citation: J Periodontal Res. 2006 Aug;41(4):273-9
references.pmid: 16827720
references.citation: J Periodontol. 2000 Jan;71(1):31-43
references.pmid: 10695936
references.citation: J Periodontol. 2000 Dec;71(12):1829-39
references.pmid: 11156039
references.citation: Anat Rec. 2001 Dec 1;264(4):438-46
references.pmid: 11745098
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004 Feb;97(2):239-45
references.pmid: 14970783
references.citation: J Clin Periodontol. 1997 Sep;24(9 Pt 2):658-68
references.pmid: 9310870
references.citation: J Histochem Cytochem. 2002 Apr;50(4):567-74
references.pmid: 11897810
references.citation: Clin Oral Investig. 2004 Sep;8(3):165-71
references.pmid: 15060819
references.citation: J Dent Res. 1998 Feb;77(2):377-86
references.pmid: 9465170
references.citation: J Periodontal Res. 2003 Jun;38(3):333-42
references.pmid: 12753373