title: Molecular mechanism of the inhibition effect of Lipoxin A4 on corneal dissolving pathology process.
abstract: OBJECTIVE
  Excessive dissolve of corneal tissue induced by MMPs which were activated by cytokins and chemokines will lead to corneal ulcer. The molecular mechanism of Lipoxin A4 (LXA4) on corneal collagen degradation in three dimensions was investigated.
  
  
  METHODS
  Rabbit corneal fibroblasts were harvested and suspended in serum-free MEM. Type I collagen, DMEM, collagen reconstitution buffer and corneal fibroblast suspension were mixed on ice. The resultant mixture solidified in an incubator, after which test reagents and plasminogen was overlaid and the cultures were returned to the incubator. The supernatants from collagen gel incubations were collected and the amount of hydroxyproline in the hydrolysate was measured. Immunoblot analysis of MMP-1, -3 and TMMP-1,-2 was performed. MMP-2,-9 was detected by the method of Gelatin zymography. Cytotoxicity assay was measured.
  
  
  RESULTS
  LXA4 inhibited corneal collagen degradation in a dose and time manner. LXA4 inhibited the IL-1β induced increases in the pro-MMP-1, -2, -3, -9 and active MMP-1, -2, -3, -9 in a concentration dependent manner. LXA4 could also inhibit the IL-1β induced increases in TIMP-1, -2.
  
  
  CONCLUSIONS
  As a potent anti-inflammation reagent, LXA4 can inhibit corneal collagen degradation induced by IL-1β in corneal fibroblasts thus inhibiting corneal dissolving pathology process.
authors.affiliation: Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhou
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Hao
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Bi
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Zhang
keywords: IL-1β; collagen; cornea; dissolution; lipoxin A4
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