title: Identification and functional characterization of novel MATE1 genetic variations in Koreans.
abstract: Multidrug and toxin extrusion 1 (MATE1, SLC47A1), an organic cation transporter, plays an important role in the renal and biliary elimination of various clinical drugs, including the anti-diabetic drug metformin. The goal of this study was to identify and characterize novel genetic variants of MATE1. Five variants in the promoter region and two nonsynonymous variants, p.D64G and p.L125F, were identified in 48 DNA samples from healthy Koreans. MATE1 promoter haplotype 3 containing g.-1975C>A showed a significant increase in reporter activity. Three transcription factors, Nkx-2.5, SREBP-1, and USF-1 were predicted to bind to the promoter in the region of g.-1975C>A. Results from electrophoretic mobility shift assays showed that the g.-1975A allele exhibits greater binding affinity to all of these transcription factors than the g.-1975C allele. In particular, we found that Nkx-2.5 and USF-1 induce MATE1 transcription. Our study suggests that the common promoter haplotype of MATE1 changes MATE1 transcriptional activity regulated by Nkx-2.5, SREBP-1, and USF-1.
authors.affiliation: Department of Pharmacology, Tissue Injury Defense Research Center, School of Medicine, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Tae Hee
authors.initials: TH
authors.lastname: Kim
authors.forename: Kyoung Hee
authors.initials: KH
authors.lastname: Kim
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Siyoon
authors.initials: S
authors.lastname: Kim
authors.forename: Ji Ha
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population / Q000379:methods; D015894:Genome, Human; D045325:HCT116 Cells; D006239:Haplotypes; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D056910:Republic of Korea; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D051720:Upstream Stimulatory Factors / Q000235:genetics
chemical_list: D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; C095552:NKX2-5 protein, human; D027701:Organic Cation Transport Proteins; C521869:SLC47A1 protein, human; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors