title: Establishment of a novel model of peritoneal carcinomatosis of the peritoneal extension type.
abstract: OBJECTIVE
  Patients with scirrhous carcinoma of the gastrointestinal tract frequently develop peritoneal carcinomatosis-particularly of the peritoneal extension type (PET), which has a bad prognosis. We developed a novel animal model, suitable for testing treatments for PET.
  
  
  METHODS
  In order to develop the model, we scraped the entire peritoneum of Fischer 344 rats with sterile cotton swabs and injected 1 × 10(6) cells of the RCN-9 cell type into the peritoneal cavity.
  
  
  RESULTS
  In the novel experimental model, RCN-9 cells adhered only to the exposed basement membrane. The submesothelial layer and fibroblasts in the submesothelial layer grew and increased to a maximum at day 7, then decreased during late-phase peritoneal carcinomatosis. At day 14, RCN-9 cells coated the peritoneum in a manner similar to PET.
  
  
  CONCLUSIONS
  We successfully established a novel animal model of peritoneal carcinomatosis that mimics clinicopathological features of PET. Fibroblasts in the submesothelial layer potentially play an important role in peritoneal carcinomatosis.
authors.affiliation: Department of Surgery, Kinki University Faculty of Medicine, Osaka-Sayama, Osaka, Japan. imano@med.kindai.ac.jp
authors.forename: Motohiro
authors.initials: M
authors.lastname: Imano
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Itoh
authors.forename: Takao
authors.initials: T
authors.lastname: Satou
authors.forename: Akira
authors.initials: A
authors.lastname: Kido
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tsubaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yasuda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kato
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Imamoto
authors.forename: Shozo
authors.initials: S
authors.lastname: Nishida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Takeyama
authors.forename: Kiyokata
authors.initials: K
authors.lastname: Okuno
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiozaki
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D003110:Colonic Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D010534:Peritoneal Neoplasms / Q000556:secondary*; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured