title: Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces.
abstract: We describe an innovation in the immobilization, culture, and imaging of cells in calcium alginate within microfluidic devices. This technique allows unprecedented optical access to the entirety of the calcium alginate hydrogel, enabling observation of growth and behavior in a chemical and mechanical environment favored by many kinds of cells.
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
authors.forename: Jordan F
authors.initials: JF
authors.lastname: Betz
authors.forename: Yi
authors.initials: Y
authors.lastname: Cheng
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Tsao
authors.forename: Amin
authors.initials: A
authors.lastname: Zargar
authors.forename: Hsuan-Chen
authors.initials: HC
authors.lastname: Wu
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Luo
authors.forename: Gregory F
authors.initials: GF
authors.lastname: Payne
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
authors.forename: Gary W
authors.initials: GW
authors.lastname: Rubloff
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D004566:Electrodes; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D013499:Surface Properties
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D002118:Calcium