title: Characterization of the guinea pig CMV gH/gL/GP129/GP131/GP133 complex in infection and spread.
abstract: In human cytomegalovirus (HCMV), the UL128-131A locus plays an essential role in cellular tropism and spread. Here, we report the complete annotation of the GP129-133 locus from guinea pig cytomegalovirus (GPCMV) and the discovery of the UL131A homolog, named GP133. We have found that similar to HCMV the GP129-133 proteins form a pentamer complex with the GPCMV glycoproteins gH and gL. In addition, we find that the GP129-133 proteins play a critical role in entry as the GP129-133 deletion mutant shows a defect in both endothelial and fibroblast cell entry. Although the GP129-133 deletion strain can propagate in vitro, we find that the deletion fails to spread in vivo. Interestingly, the wildtype strain can spontaneously give rise to the GP129-133 deletion strain during in vivo spread, suggesting genetic instability at this locus.
authors.affiliation: Department of Infectious Diseases, Genentech, Inc./Hoffmann-LaRoche, Ltd., 1 DNA Way, South San Francisco, CA 94080, United States.
authors.forename: Marcy
authors.initials: M
authors.lastname: Auerbach
authors.forename: Donghong
authors.initials: D
authors.lastname: Yan
authors.forename: Ashley
authors.initials: A
authors.lastname: Fouts
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Alberto
authors.initials: A
authors.lastname: Estevez
authors.forename: Cary D
authors.initials: CD
authors.lastname: Austin
authors.forename: Fernando
authors.initials: F
authors.lastname: Bazan
authors.forename: Becket
authors.initials: B
authors.lastname: Feierbach
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics; D042783:Endothelial Cells / Q000821:virology; D005347:Fibroblasts / Q000821:virology; D017353:Gene Deletion; D006168:Guinea Pigs; D008297:Male; D055503:Protein Multimerization; D019211:Roseolovirus / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D014764:Viral Proteins