title: In situ IgM production and clonal expansion of B-1 cells in peritoneal cavity promote elimination of C. albicans infection in IgH transgenic mice with VH derived from a natural antibody.
abstract: B-1 cells are innate-like cells that play important roles in host defense against infection. However, the function of B-1 cells in fungi infection remains unclear. Previously we produced IgH transgenic mice TgVH3B4 with VH derived from a natural antibody 3B4 that can identify C. albicans, and found that TgVH3B4 mice were resistant to intraperitoneal (i. p.) and intravenous C. albicans infection. Most of the peritoneal cavity (PEC) B-1 cells in TgVH3B4 mice express transgenic BCR that binds C. albicans. In the present study, we explored the response of B-1 cells to C. albicans infection by applying i. p. inoculation of fungi in TgVH3B4 mice. We found that C. albicans was cleared more efficiently in TgVH3B4 mice after i. p. inoculation than that of littermate control. The level of C. albicans-reactive IgM in PEC of TgVH3B4 mice was much higher than that of control, and the number of B-1a B cells was also elevated in TgVH3B4 mice, which was mainly due to enhanced proliferation of B-1 cells. Additionally, numbers of C. albicans-specific B cells increased greatly in TgVH3B4 mice after C. albicans inoculation. Our data suggested that in situ IgM production and clonal expansion of B-1 cells in PEC participate in host defense against C. albicans infection.
authors.affiliation: Department of Dermatology, General Hospital of the Air Force, Beijing, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Tian
authors.forename: Meng
authors.initials: M
authors.lastname: Fu
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.forename: Jingang
authors.initials: J
authors.lastname: An
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D010529:Peritoneal Cavity / Q000382:microbiology*
chemical_list: D007075:Immunoglobulin M
references.citation: J Exp Med. 2003 Jan 6;197(1):87-99
references.pmid: 12515816
references.citation: Immunity. 2005 Jul;23(1):7-18
references.pmid: 16039575
references.citation: Mycopathologia. 1985 Aug;91(2):79-85
references.pmid: 3900732
references.citation: Apoptosis. 2007 Feb;12(2):355-62
references.pmid: 17191117
references.citation: J Infect Dis. 1996 Sep;174(3):589-97
references.pmid: 8769618
references.citation: Biomed Pharmacother. 2004 Jun;58(5):276-81
references.pmid: 15194162
references.citation: Annu Rev Immunol. 2001;19:595-621
references.pmid: 11244048
references.citation: Cell Microbiol. 2007 Feb;9(2):306-15
references.pmid: 16925788
references.citation: Science. 1999 Dec 10;286(5447):2156-9
references.pmid: 10591647
references.citation: Int Immunol. 1989;1(1):75-84
references.pmid: 2487677
references.citation: FEMS Immunol Med Microbiol. 2003 Mar 20;35(2):93-8
references.pmid: 12628543
references.citation: J Immunol. 2005 Jun 1;174(11):6918-26
references.pmid: 15905534
references.citation: Immunity. 2002 Jun;16(6):791-802
references.pmid: 12121661
references.citation: Annu Rev Immunol. 2002;20:253-300
references.pmid: 11861604
references.citation: Infect Immun. 1997 Sep;65(9):3822-7
references.pmid: 9284158
references.citation: Nat Immunol. 2000 Nov;1(5):441-6
references.pmid: 11062505
references.citation: J Immunol. 2003 Nov 15;171(10):5406-14
references.pmid: 14607944
references.citation: Infect Immun. 2004 Jan;72(1):209-18
references.pmid: 14688098
references.citation: Mol Immunol. 2000 Dec;37(18):1141-9
references.pmid: 11451419
references.citation: Infect Immun. 2006 Jan;74(1):362-9
references.pmid: 16368991
references.citation: J Exp Med. 2000 May 1;191(9):1477-86
references.pmid: 10790423
references.citation: Science. 1999 Jul 2;285(5424):113-6
references.pmid: 10390361
references.citation: J Exp Med. 2000 Feb 21;191(4):731-6
references.pmid: 10684864
references.citation: Mol Immunol. 2009 Mar;46(6):1120-8
references.pmid: 19136153
references.citation: J Exp Med. 1998 Dec 21;188(12):2381-6
references.pmid: 9858525
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3886-91
references.pmid: 14999103
references.citation: J Exp Med. 2000 Jul 17;192(2):271-80
references.pmid: 10899913
references.citation: Immunity. 2000 Jan;12(1):39-49
references.pmid: 10661404
references.citation: Immunity. 2001 May;14(5):617-29
references.pmid: 11371363
references.citation: J Autoimmun. 2007 Dec;29(4):236-45
references.pmid: 17889506
references.citation: J Lipid Res. 2005 Jul;46(7):1353-63
references.pmid: 15897601
references.citation: Infect Immun. 2001 Oct;69(10):6456-62
references.pmid: 11553590