title: Autologous uncultured bone marrow-derived mononuclear cells and modified cannulated screw in repair of femoral neck fracture.
abstract: The purpose of this study was to assess whether autologous uncultured bone marrow-derived mononuclear cells (BMMNCs) combined with modified cannulated screw would accelerate the healing of canine femoral neck fracture. BMMNCs were encapsulated within fibrin glue (FG) and implanted into the fractured femoral neck via modified cannulated screw in experiment group, and the control group was treated by modified cannulated screw. Gross observation, radiological examination, histological analysis, and blood vessel microdensity counting were used to compare bone healing of each group at 1, 2, and 3 months. FG was confirmed as an ideal cell-delivery vehicle for BMMNCs proliferation and differentiation in vitro testing. In vivo animal testing, faster new bone formation and fracture healing were confirmed by gross observation, radiological examination, histological analysis in experimental group than in control group at all times points. The blood vessel microdensity counting increased gradually both in the experimental group and control group, but was more obviously in experimental group at 3 months (p < 0.01). These data suggest that autologous BMMNCs combined with modified cannulated screw treatment is an effective therapy for femoral neck fracture and thus, may be an option for clinical applications.
authors.affiliation: Department of Orthopaedics, Chinese PLA General Hospital, Beijing, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Licheng
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lihai
authors.forename: Xu
authors.initials: X
authors.lastname: Meng
authors.forename: Yao
authors.initials: Y
authors.lastname: Qi
authors.forename: Tang
authors.initials: T
authors.lastname: Peifu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D001863:Bone Screws; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004285:Dogs; D005265:Femoral Neck Fractures / Q000473:pathology / Q000628:therapy*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D017102:Fracture Healing / Q000187:drug effects*; D005596:Fractures, Closed / Q000473:pathology / Q000628:therapy*; D008297:Male; D008833:Microcirculation; D009000:Monocytes / Q000473:pathology / Q000637:transplantation*; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D015718:Fibrin Tissue Adhesive