title: Exposure to 2,4-dichlorophenoxyacetic acid alters glucose metabolism in immature rat Sertoli cells.
abstract: The purpose of this study was to determine the effects of 2,4-D, an herbicide used worldwide also known as endocrine disruptor, in Sertoli cell (SC) metabolism. Immature rat SCs were maintained 50h under basal conditions or exposed to 2,4-D (100nM, 10μM and 1mM). SCs exposed to 10μM and 1mM of 2,4-D presented lower intracellular glucose and lactate content. Exposure to 10μM of 2,4-D induced a significant decrease in glucose transporter-3 mRNA levels and phosphofructokinase-1 mRNA levels decreased in cells exposed to 100nM and 10μM of 2,4-D. Exposure to 100nM and 10μM also induced a decrease in lactate dehydrogenase (LDH) mRNA levels while the LDH protein levels were only decreased in cells exposed to 1mM of 2,4-D. Exposure to 2,4-D altered glucose uptake and metabolization in SCs, as well as lactate metabolism and export that may result in impaired spermatogenesis.
authors.affiliation: CICS - UBI - Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal. alvesmarc@gmail.com
authors.forename: M G
authors.initials: MG
authors.lastname: Alves
authors.forename: A
authors.initials: A
authors.lastname: Neuhaus-Oliveira
authors.forename: P I
authors.initials: PI
authors.lastname: Moreira
authors.forename: S
authors.initials: S
authors.lastname: Socorro
authors.forename: P F
authors.initials: PF
authors.lastname: Oliveira
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity*; D000409:Alanine / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D052244:Endocrine Disruptors / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D051274:Glucose Transporter Type 3; D006540:Herbicides; D027501:Monocarboxylic Acid Transporters; D009124:Muscle Proteins; C516342:Slc16a3 protein, rat; C495690:Slc2a3 protein, rat; D015084:2,4-Dichlorophenoxyacetic Acid; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D010732:Phosphofructokinase-1; D005947:Glucose; D000409:Alanine