title: Transcriptome analysis of spermatogenically regressed, recrudescent and active phase testis of seasonally breeding wall lizards Hemidactylus flaviviridis.
abstract: BACKGROUND
  Reptiles are phylogenically important group of organisms as mammals have evolved from them. Wall lizard testis exhibits clearly distinct morphology during various phases of a reproductive cycle making them an interesting model to study regulation of spermatogenesis. Studies on reptile spermatogenesis are negligible hence this study will prove to be an important resource.
  
  
  RESULTS
  Histological analyses show complete regression of seminiferous tubules during regressed phase with retracted Sertoli cells and spermatognia. In the recrudescent phase, regressed testis regain cellular activity showing presence of normal Sertoli cells and developing germ cells. In the active phase, testis reaches up to its maximum size with enlarged seminiferous tubules and presence of sperm in seminiferous lumen. Total RNA extracted from whole testis of regressed, recrudescent and active phase of wall lizard was hybridized on Mouse Whole Genome 8×60 K format gene chip. Microarray data from regressed phase was deemed as control group. Microarray data were validated by assessing the expression of some selected genes using Quantitative Real-Time PCR. The genes prominently expressed in recrudescent and active phase testis are cytoskeleton organization GO 0005856, cell growth GO 0045927, GTpase regulator activity GO: 0030695, transcription GO: 0006352, apoptosis GO: 0006915 and many other biological processes. The genes showing higher expression in regressed phase belonged to functional categories such as negative regulation of macromolecule metabolic process GO: 0010605, negative regulation of gene expression GO: 0010629 and maintenance of stem cell niche GO: 0045165.
  
  
  CONCLUSIONS
  This is the first exploratory study profiling transcriptome of three drastically different conditions of any reptilian testis. The genes expressed in the testis during regressed, recrudescent and active phase of reproductive cycle are in concordance with the testis morphology during these phases. This study will pave the way for deeper insight into regulation and evolution of gene regulatory mechanisms in spermatogenesis.
authors.affiliation: Comparative Immuno-Endocrinology Laboratory, Department of Zoology, University of Delhi, Delhi, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Gautam
authors.forename: Amitabh
authors.initials: A
authors.lastname: Mathur
authors.forename: Meraj Alam
authors.initials: MA
authors.lastname: Khan
authors.forename: Subeer S
authors.initials: SS
authors.lastname: Majumdar
authors.forename: Umesh
authors.initials: U
authors.lastname: Rai
mesh_terms: D000818:Animals; D001947:Breeding; D016000:Cluster Analysis; D019295:Computational Biology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008116:Lizards / Q000502:physiology*; D008297:Male; D058977:Molecular Sequence Annotation; D015203:Reproducibility of Results; D012098:Reproduction / Q000235:genetics; D012621:Seasons; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000502:physiology*
references.citation: Cancer Res. 2007 Mar 15;67(6):2552-8
references.pmid: 17363573
references.citation: Cell Cycle. 2006 Nov 1;5(21):2452-6
references.pmid: 17102626
references.citation: BMC Genomics. 2008 Apr 16;9:176
references.pmid: 18416834
references.citation: Mol Hum Reprod. 2007 Jan;13(1):33-43
references.pmid: 17114209
references.citation: Mol Ecol. 2007 Apr;16(8):1749-63
references.pmid: 17402988
references.citation: Genome Res. 2008 Sep;18(9):1509-17
references.pmid: 18550803
references.citation: Oncogene. 2008 Nov 24;27(55):6908-19
references.pmid: 19029933
references.citation: Aging Cell. 2006 Oct;5(5):413-22
references.pmid: 16939484
references.citation: Cytoskeleton (Hoboken). 2010 Sep;67(9):564-72
references.pmid: 20662086
references.citation: Bioinformatics. 2003 Mar 22;19(5):571-8
references.pmid: 12651714
references.citation: Immunol Cell Biol. 2008 Jan;86(1):47-53
references.pmid: 17998914
references.citation: Biol Reprod. 2010 Jan;82(1):202-13
references.pmid: 19741204
references.citation: Biol Reprod. 2004 Jul;71(1):319-30
references.pmid: 15028632
references.citation: Transplant Proc. 2008 Mar;40(2):598-602
references.pmid: 18374140
references.citation: Mol Biol Cell. 2009 Aug;20(15):3543-51
references.pmid: 19477923
references.citation: Bioinformatics. 2002;18 Suppl 1:S164-71
references.pmid: 12169544
references.citation: Science. 2012 Mar 9;335(6073):1195-200
references.pmid: 22345399
references.citation: EMBO J. 2000 Feb 1;19(3):359-69
references.pmid: 10654935
references.citation: Nature. 2011 Mar 10;471(7337):245-8
references.pmid: 21390132
references.citation: J Exp Zool. 1998 Nov-Dec 1;282(4-5):560-617
references.pmid: 9867504
references.citation: Mol Cell. 2009 Feb 27;33(4):450-61
references.pmid: 19250906
references.citation: Endocrinology. 1988 Mar;122(3):787-94
references.pmid: 3125042
references.citation: Biochem J. 2009 Jan 1;417(1):15-27
references.pmid: 19061484
references.citation: Mol Endocrinol. 2004 Feb;18(2):422-33
references.pmid: 14605096
references.citation: Nat Protoc. 2009;4(1):44-57
references.pmid: 19131956
references.citation: Biol Reprod. 2008 Sep;79(3):475-85
references.pmid: 18480464
references.citation: Insect Mol Biol. 2007 Jun;16(3):315-24
references.pmid: 17433071
references.citation: Cell Tissue Res. 2007 Mar;327(3):583-94
references.pmid: 17096167
references.citation: Cell Growth Differ. 2000 Aug;11(8):455-65
references.pmid: 10965850
references.citation: Cancer Sci. 2009 Jul;100(7):1173-9
references.pmid: 19432890
references.citation: Stem Cells. 2010 Oct;28(10):1882-92
references.pmid: 20799334
references.citation: Mech Dev. 2003 Nov;120(11):1337-49
references.pmid: 14623442
references.citation: Development. 2011 Aug;138(16):3519-31
references.pmid: 21771810
references.citation: Int J Oncol. 2010 Oct;37(4):909-26
references.pmid: 20811713
references.citation: J Pathol. 2009 Jan;217(1):94-102
references.pmid: 18839394
references.citation: Anim Genet. 2007 Aug;38(4):319-31
references.pmid: 17596124
references.citation: J Biol Chem. 2004 Jul 2;279(27):28641-52
references.pmid: 15117962
references.citation: Nature. 2011 Aug 31;477(7366):587-91
references.pmid: 21881562
references.citation: Methods Mol Biol. 2008;410:81-110
references.pmid: 18642596
references.citation: Mol Endocrinol. 2004 Nov;18(11):2805-16
references.pmid: 15297604
references.citation: PLoS One. 2008;3(10):e3467
references.pmid: 18941544
references.citation: J Biol Chem. 2012 Apr 27;287(18):14621-30
references.pmid: 22411991
references.citation: J Biol Chem. 2003 Mar 7;278(10):7783-9
references.pmid: 12488439
references.citation: BMC Bioinformatics. 2004 Feb 18;5:16
references.pmid: 14975175
references.citation: Gen Comp Endocrinol. 1970 Feb;14(1):15-24
references.pmid: 5435509
references.citation: Am J Physiol Renal Physiol. 2007 Mar;292(3):F981-92
references.pmid: 17148781
references.citation: Biol Reprod. 2005 Feb;72(2):492-501
references.pmid: 15496517
references.citation: Mol Genet Metab. 2009 Jun;97(2):155-62
references.pmid: 19329343
references.citation: Proc Biol Sci. 2005 Feb 7;272(1560):289-94
references.pmid: 15705554
references.citation: Biol Reprod. 2009 Aug;81(2):438-43
references.pmid: 19369649
references.citation: Blood Cells Mol Dis. 2007 Nov-Dec;39(3):340-3
references.pmid: 17669672
references.citation: Adv Immunol. 2011;111:179-206
references.pmid: 21970955
references.citation: Nucleic Acids Res. 1994 Nov 25;22(23):4914-21
references.pmid: 7800480
references.citation: Mol Cell Endocrinol. 2010 Feb 5;315(1-2):49-56
references.pmid: 19682538
references.citation: Genome Biol. 2007;8(5):R72
references.pmid: 17475010
references.citation: EMBO J. 2011 Aug 02;30(17):3635-46
references.pmid: 21811233
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jan;151(1):51-6
references.pmid: 19703584
references.citation: Eur J Morphol. 1995 Nov;33(5):443-55
references.pmid: 8907557
references.citation: Reproduction. 2006 Jul;132(1):11-9
references.pmid: 16816329
references.citation: Cell Tissue Res. 1975 Nov 19;163(4):545-60
references.pmid: 1201592
references.citation: EMBO J. 1985 Jun;4(6):1515-21
references.pmid: 4040852
references.citation: Gen Comp Endocrinol. 1964 Oct;4:550-62
references.pmid: 14217824
references.citation: Nat Cell Biol. 2009 Mar;11(3):278-85
references.pmid: 19182792
references.citation: Clin Transplant. 2004;18 Suppl 12:76-80
references.pmid: 15217413
references.citation: Dev Biol. 2010 Jul 1;343(1-2):63-70
references.pmid: 20417196
references.citation: Cell Mol Life Sci. 2005 Nov;62(22):2679-93
references.pmid: 16261251
references.citation: Biochemistry. 1995 Dec 19;34(50):16503-8
references.pmid: 8845379
references.citation: J Clin Endocrinol Metab. 2006 Mar;91(3):1062-8
references.pmid: 16368746
references.citation: Pflugers Arch. 2009 Nov;459(1):183-9
references.pmid: 19585141
references.citation: Bioinformatics. 2002 Apr;18(4):546-54
references.pmid: 12016052
references.citation: Cell Growth Differ. 1995 Oct;6(10):1213-24
references.pmid: 8845298
references.citation: Nat Cell Biol. 2010 Jun;12(6):618-24
references.pmid: 20473294
references.citation: Leuk Res. 2002 Oct;26(10):945-54
references.pmid: 12163057
references.citation: Am J Pathol. 2003 Dec;163(6):2149-54
references.pmid: 14633588
references.citation: Bioinformatics. 2001 Sep;17(9):763-74
references.pmid: 11590094
references.citation: Mol Biol Cell. 2006 Mar;17(3):1204-17
references.pmid: 16394104
references.citation: Fertil Steril. 2012 Nov;98(5):1308-17.e1
references.pmid: 22901849
references.citation: Cell Stem Cell. 2010 Sep 3;7(3):288-98
references.pmid: 20804966
references.citation: Nat Genet. 2010 Sep;42(9):742-4
references.pmid: 20676097
references.citation: J Comp Physiol B. 2011 Jul;181(5):575-94
references.pmid: 21573966
references.citation: Mol Ecol. 2009 May;18(10):2092-102
references.pmid: 19389180
references.citation: Nature. 2010 Sep 16;467(7313):333-7
references.pmid: 20844538
references.citation: Fish Shellfish Immunol. 2009 May;26(5):699-706
references.pmid: 19264136
references.citation: Differentiation. 2008 Jul;76(6):717-27
references.pmid: 18557759
references.citation: BMC Mol Biol. 2006 Jan 31;7:3
references.pmid: 16448564
references.citation: J Biol Chem. 2000 Dec 15;275(50):39762-6
references.pmid: 10995736
references.citation: Epigenetics Chromatin. 2010 Apr 27;3(1):9
references.pmid: 20423503
references.citation: Mol Cell Biol. 2009 Aug;29(15):4144-55
references.pmid: 19470757