title: A fast, reliable, ultra high performance liquid chromatography method for the simultaneous determination of amino acids, biogenic amines and ammonium ions in cheese, using diethyl ethoxymethylenemalonate as a derivatising agent.
abstract: Derivatisation treatment with diethyl ethoxymethylenemalonate followed by ultra-HPLC allowed the simultaneous quantification of 22 amino acids, 7 biogenic amines and ammonium ions in cheese samples in under 10 min. This is the fastest elution time ever reported for such a resolution. The proposed method shows good linearity (R(2)>0.995) and sensitivity (detection limit 0.08-3.91 μM; quantification limit <13.02 μM). Intra- and inter-day repeatability ranged from 0.35% to 1.25% and from 0.85% to 5.2%, respectively. No significant effect of the cheese matrix was observed.
authors.affiliation: Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300-Villaviciosa, Asturias, Spain. bredruel@ipla.csic.es
authors.forename: Begoña
authors.initials: B
authors.lastname: Redruello
authors.forename: Victor
authors.initials: V
authors.lastname: Ladero
authors.forename: Isabel
authors.initials: I
authors.lastname: Cuesta
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Álvarez-Buylla
authors.forename: María Cruz
authors.initials: MC
authors.lastname: Martín
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alvarez
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001679:Biogenic Amines / Q000032:analysis*; D002611:Cheese / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008314:Malonates / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D000596:Amino Acids; D001679:Biogenic Amines; D008314:Malonates; D000644:Quaternary Ammonium Compounds; C044472:diethyl (ethoxymethylene)malonate