title: A versatile platform for highly sensitive detection of kinase activity based on metal ion-mediated FRET using an anionic conjugated polymer.
abstract: An anionic fluorescent conjugated polymer (PFPaa), coupled with the metal ion-mediated fluorescence resonance energy transfer, has been used to design a versatile platform for homogeneous detection of protein kinase activity.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Chenghui
authors.initials: C
authors.lastname: Liu
authors.forename: Teng
authors.initials: T
authors.lastname: Yang
authors.forename: Fangfang
authors.initials: F
authors.lastname: Wang
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D000838:Anions; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D007546:Isoquinolines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D047428:Protein Kinase Inhibitors / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry
chemical_list: D000838:Anions; D005449:Fluorenes; D005456:Fluorescent Dyes; D007546:Isoquinolines; D011108:Polymers; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D017868:Cyclic AMP-Dependent Protein Kinases; D016650:Fluorescein-5-isothiocyanate; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide