title: Repeated administration of tribromoethanol in C57BL/6NHsd mice.
abstract: We evaluated the effect of repeated intraperitoneal administration of tribromoethanol on various parameters in C57BL/6NHsd mice. Mice (n = 68) were randomly assigned to 1 of 7 groups to receive tribromoethanol (500 mg/kg IP) on day 0 or days 0 and 8; vehicle (tert-amyl alcohol in sterile water) only on day 0 or days 0 and 8; sterile water injection on day 0 or days 0 and 8; or no treatment. A single dose of tribromoethanol failed to produce loss of pedal reflex and had no effect on median food and water consumption but altered median body weight on days 1 through 4 when compared with that in mice that received vehicle only or no treatment. Median body weight did not differ between mice that received a single dose of tribromoethanol and those that received an injection of water. Among mice given 2 doses of tribromoethanol, induction time, anesthetic duration, and recovery time varied widely. Repeated administration of tribromoethanol had no effect on median food and water consumption or body weight compared with those in controls. Median liver weight was significantly greater in mice that received 2 doses compared with a single dose of tribromoethanol. Median liver weight did not differ between untreated mice and those that received tribromoethanol. No significant organ or tissue pathology was observed in any study animal. Although tribromoethanol did not produce morbidity, mortality, or pathologic changes in treated animals, we urge caution in use of tribromoethanol in C57BL/6NHsd mice due to its variable anesthetic effectiveness.
authors.affiliation: Department of Biomedical and Diagnostic Sciences, Institute of Agriculture, Knoxville, TN, USA. wahill@utk.edu
authors.forename: William A
authors.initials: WA
authors.lastname: Hill
authors.forename: Jacquelyn T
authors.initials: JT
authors.lastname: Tubbs
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Carter
authors.forename: Jane A
authors.initials: JA
authors.lastname: Czarra
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Newkirk
authors.forename: Tim E
authors.initials: TE
authors.lastname: Sparer
authors.forename: Barton
authors.initials: B
authors.lastname: Rohrbach
authors.forename: Christine M
authors.initials: CM
authors.lastname: Egger
mesh_terms: D000777:Anesthetics / Q000008:administration & dosage / Q000009:adverse effects*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D000439:Pentanols / Q000008:administration & dosage; D011897:Random Allocation
chemical_list: D000777:Anesthetics; D000439:Pentanols; C062527:tribromoethanol; D000431:Ethanol; C032765:tert-amyl alcohol
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