title: Simultaneous separation of water- and fat-soluble vitamins in isocratic pressure-assisted capillary electrochromatography using a methacrylate-based monolithic column.
abstract: A method of simultaneous separation of water- and fat-soluble vitamins using pressure-assisted CEC with a methacrylate-based capillary monolithic column was developed. In the proposed method, water-soluble vitamins were mainly separated electrophoretically, while fat soluble-ones were separated chromatographically by the interaction with a methacrylate-based monolith. A mixture of six water-soluble and four fat-soluble vitamins was separated simultaneously within 20 min with an isocratic elution using 1 M formic acid (pH 1.9)/acetonitrile (30:70, v/v) containing 10 mM ammonium formate as a mobile phase. When the method was applied to a commercial multivitamin tablet and a spiked one, the vitamins were successfully analyzed, and no influence of the matrix contained in the tablet was observed.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yamada
authors.forename: Shinya
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohtani
mesh_terms: D000327:Adsorption; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D005223:Fats / Q000032:analysis; D008689:Methacrylates / Q000737:chemistry*; D014815:Vitamins / Q000032:analysis / Q000302:isolation & purification*; D014867:Water / Q000032:analysis
chemical_list: D005223:Fats; D008689:Methacrylates; D014815:Vitamins; D014867:Water