title: Immunization with recombinant prion protein leads to partial protection in a murine model of TSEs through a novel mechanism.
abstract: Transmissible spongiform encephalopathies are neurodegenerative diseases, which despite fervent research remain incurable. Immunization approaches have shown great potential at providing protection, however tolerance effects hamper active immunization protocols. In this study we evaluated the antigenic potential of various forms of recombinant murine prion protein and estimated their protective efficacy in a mouse model of prion diseases. One of the forms tested provided a significant elongation of survival interval. The elongation was mediated via an acute depletion of mature follicular dendritic cells, which are associated with propagation of the prion infectious agent in the periphery and in part to the development of humoral immunity against prion protein. This unprecedented result could offer new strategies for protection against transmissible encephalopathies as well as other diseases associated with follicular dendritic cells.
authors.affiliation: Laboratory of Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Xanthopoulos
authors.forename: Rosa
authors.initials: R
authors.lastname: Lagoudaki
authors.forename: Anastasia
authors.initials: A
authors.lastname: Kontana
authors.forename: Christos
authors.initials: C
authors.lastname: Kyratsous
authors.forename: Christos
authors.initials: C
authors.lastname: Panagiotidis
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Grigoriadis
authors.forename: Minas
authors.initials: M
authors.lastname: Yiangou
authors.forename: Theodoros
authors.initials: T
authors.lastname: Sklaviadis
mesh_terms: D000818:Animals; D020566:Dendritic Cells, Follicular / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D056724:Immunity, Humoral / Q000187:drug effects; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D017096:Prion Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D011328:Prions / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D016019:Survival Analysis
chemical_list: D011328:Prions; D011994:Recombinant Proteins
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