title: Wacław Szybalski's contribution to immunotherapy: HGPRT mutation & HAT selection as first steps to gene therapy and hybrid techniques in mammalian cells.
abstract: In this report we describe Wacław Szybalski's fundamental contribution to gene therapy and immunotherapy. His 1962 PNAS paper (Szybalska and Szybalski, 1962) documented the first successful gene repair in mammalian cells. Furthermore, this was also the first report on the HAT selection method used later in many applications. Most importantly, somatic cell fusion and HAT selection were subsequently used to develop monoclonal antibody technology, which contributed significantly to the progress of today's medicine.
authors.affiliation: Cell Biology Unit, Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Jacek J
authors.initials: JJ
authors.lastname: Bigda
authors.forename: Patrycja
authors.initials: P
authors.lastname: Koszałka
mesh_terms: D000630:Aminopterin; D000818:Animals; D000911:Antibodies, Monoclonal / Q000266:history; D000971:Antineoplastic Combined Chemotherapy Protocols; D002459:Cell Fusion; D015316:Genetic Therapy / Q000266:history*; D049673:History, 20th Century; D006801:Humans; D006822:Hybrid Cells; D019271:Hypoxanthine; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D007167:Immunotherapy / Q000266:history*; D008322:Mammals / Q000235:genetics; D009154:Mutation; D013936:Thymidine
chemical_list: D000911:Antibodies, Monoclonal; D019271:Hypoxanthine; D007041:Hypoxanthine Phosphoribosyltransferase; D000630:Aminopterin; D013936:Thymidine
keywords: ADA-SCID; DNAse; ES cells; Gene therapy; Genetic transformation; HAT; HAT selection; HPRT; IMP; Monoclonal antibodies; RT-PCR; Wacław Szybalski; adenosine deaminase-deficient severe combined immunodeficiency; deoxyribonuclease; embryonic stem cells; hypoxanthine, aminopterin, thymidine; hypoxanthine-guanine phosphoribosyl transferase; inosine monophosphate; reverse transcription polymerase chain reaction