title: Characterization and functional analysis of the potato pollen-specific microtubule-associated protein SBgLR in tobacco.
abstract: Microtubule-associated proteins play a crucial role in the regulation of microtubule dynamics, and are very important for plant cell and organ development. SBgLR is a potato pollen-specific protein, with five imperfect V-V-E-K-K-N/E-E repetitive motifs that are responsible for microtubule binding activity. In present study, SBgLR showed typical microtubule-associated protein characteristics; it bound tubulin and microtubules, and colocalized with microtubules in vitro. We also found that SBgLR could form oligomers, and that both the SBgLR monomers and oligomers bundle microtubules in vitro. Constitutive expression of SBgLR in tobacco caused curving and right-handed twisting root growth, abnormal directional cell expansion and cell layer arrangement, and pollen abortion. Immunofluorescence staining assays revealed that microtubule organization is altered in root epidermal cells in SBgLR-overexpressing lines. These suggest that SBgLR functions as a microtubule-associated protein in pollen development. Our results indicate that normal organization of MTs may be crucial for pollen development.
authors.affiliation: State Key Laboratory for Agro-biotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jingjuan
authors.initials: J
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D049109:Cell Proliferation; D015870:Gene Expression; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011058:Pollen / Q000648:ultrastructure; D011485:Protein Binding; D055503:Protein Multimerization; D011994:Recombinant Proteins; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D014404:Tubulin
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