title: Crystallization and X-ray structure determination of a thermoalkalophilic lipase from Geobacillus SBS-4S.
abstract: A thermoalkalophilic lipase (LIPSBS) from the newly isolated Geobacillus strain SBS-4S which hydrolyzes a wide range of fatty acids has been characterized. In the present study, the crystallization of purified LIPSBS using the sitting-drop vapour-diffusion method and its X-ray diffraction studies are described. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 55.13, b = 71.75, c = 126.26 Å. The structure was determined at 1.6 Å resolution by the molecular-replacement method using the lipase from G. stearothermophilus L1 as a model.
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Tayyab
authors.forename: Naeem
authors.initials: N
authors.lastname: Rashid
authors.forename: Clement
authors.initials: C
authors.lastname: Angkawidjaja
authors.forename: Shigenori
authors.initials: S
authors.lastname: Kanaya
authors.forename: Muhammad
authors.initials: M
authors.lastname: Akhtar
mesh_terms: D018360:Crystallography, X-Ray; D056506:Geobacillus / Q000201:enzymology*; D008049:Lipase / Q000737:chemistry*; D008958:Models, Molecular; D017434:Protein Structure, Tertiary
chemical_list: D008049:Lipase
keywords: Geobacillus SBS-4S; crystal structure; lipases; molecular replacement; thermoalkalophilic
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
references.pmid: 20057045
references.citation: J Biol Chem. 2002 May 10;277(19):17041-7
references.pmid: 11859083
references.citation: Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21
references.pmid: 9757107
references.citation: Proteins. 2003 Feb 15;50(3):437-50
references.pmid: 12557186
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: J Biol Chem. 2009 Feb 13;284(7):4365-72
references.pmid: 19056729
references.citation: Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
references.pmid: 15572765
references.citation: J Biosci Bioeng. 2011 Mar;111(3):272-8
references.pmid: 21185780
references.citation: Annu Rev Microbiol. 1999;53:315-51
references.pmid: 10547694
references.citation: Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
references.pmid: 21460454