title: Winner for outstanding research in the Ph.D. category for the 2013 Society for Biomaterials meeting and exposition, April 10-13, 2013, Boston, Massachusetts: Osteogenic differentiation of adipose-derived and marrow-derived mesenchymal stem cells in modular protein/ceramic microbeads.
abstract: Modular tissue engineering applies biomaterials-based approaches to create discrete cell-seeded microenvironments, which can be further assembled into larger constructs for the repair of injured tissues. In the current study, we embedded human bone marrow-derived mesenchymal stem cells (MSC) and human adipose-derived stem cells (ASC) in collagen/fibrin (COL/FIB) and collagen/fibrin/hydroxyapatite (COL/FIB/HA) microbeads, and evaluated their suitability for bone tissue engineering applications. Microbeads were fabricated using a water-in-oil emulsification process, resulting in an average microbead diameter of approximately 130 ± 25 μm. Microbeads supported both cell viability and cell spreading of MSC and ASC over 7 days in culture. The embedded cells also began to remodel and compact the microbead matrix as demonstrated by confocal reflectance microscopy imaging. After two weeks of culture in media containing osteogenic supplements, both MSC and ASC deposited calcium mineral in COL/FIB microbeads, but not in COL/FIB/HA microbeads. There were no significant differences between MSC and ASC in any of the assays examined, suggesting that either cell type may be an appropriate cell source for orthopedic applications. This study has implications in the creation of defined microenvironments for bone repair, and in developing a modular approach for delivery of pre-differentiated cells.
authors.affiliation: Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Rameshwar R
authors.initials: RR
authors.lastname: Rao
authors.forename: Alexis W
authors.initials: AW
authors.lastname: Peterson
authors.forename: Jan P
authors.initials: JP
authors.lastname: Stegemann
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology; D001900:Boston; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D003226:Congresses as Topic; D005337:Fibrin / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018613:Microscopy, Confocal; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects*; D010316:Particle Size
chemical_list: D001672:Biocompatible Materials; D005337:Fibrin; D003094:Collagen
references.citation: Acta Biomater. 2013 Jan;9(1):4457-86
references.pmid: 22922331
references.citation: J Biomed Mater Res A. 2010 Aug;94(2):344-54
references.pmid: 20186740
references.citation: J Cell Biochem. 2011 Aug;112(8):1963-8
references.pmid: 21445861
references.citation: Acta Biomater. 2011 Jun;7(6):2410-7
references.pmid: 21345389
references.citation: J Biomed Mater Res A. 2009 Sep 15;90(4):1021-31
references.pmid: 18655148
references.citation: Tissue Eng Part C Methods. 2012 Dec;18(12):935-46
references.pmid: 22624791
references.citation: Stem Cells Dev. 2012 Sep 20;21(14):2724-52
references.pmid: 22468918
references.citation: Acta Biomater. 2011 Feb;7(2):691-9
references.pmid: 20837168
references.citation: Tissue Eng Part B Rev. 2012 Aug;18(4):258-69
references.pmid: 22224439
references.citation: J Biomed Mater Res A. 2009 Dec 15;91(4):1160-9
references.pmid: 19148925
references.citation: Cells Tissues Organs. 2013;197(5):333-43
references.pmid: 23571151
references.citation: Biotechnol Bioeng. 2005 Nov 20;92(4):492-500
references.pmid: 16080186
references.citation: Tissue Eng Part A. 2013 Sep;19(17-18):1879-88
references.pmid: 22844877
references.citation: Plast Reconstr Surg. 2012 Jun;129(6):1277-1290
references.pmid: 22634645
references.citation: J Biomed Mater Res A. 2012 Nov;100(11):2929-38
references.pmid: 22700033
references.citation: Tissue Eng Part A. 2011 Dec;17(23-24):2943-54
references.pmid: 21851269
references.citation: J Biomed Mater Res B Appl Biomater. 2006 Jan;76(1):230-9
references.pmid: 16047328
references.citation: Acta Biomater. 2012 Sep;8(9):3436-45
references.pmid: 22633970
references.citation: Spine (Phila Pa 1976). 2008 Apr 15;33(8):863-9
references.pmid: 18404105
references.citation: J Mater Sci Mater Med. 2007 Feb;18(2):225-35
references.pmid: 17323153
references.citation: Biomaterials. 1993;14(1):39-43
references.pmid: 8425023
references.citation: Tissue Eng Part A. 2012 Nov;18(21-22):2290-302
references.pmid: 22731654
references.citation: Adv Drug Deliv Rev. 2011 Apr 30;63(4-5):300-11
references.pmid: 21396416
references.citation: Cells Tissues Organs. 2003;174(3):101-9
references.pmid: 12835573
references.citation: J Biomed Mater Res B Appl Biomater. 2009 Apr;89(1):252-63
references.pmid: 18777578
references.citation: Eur Cell Mater. 2010 Oct 04;20:218-30
references.pmid: 20922667
references.citation: Soft Matter. 2009;5(7):1312-1319
references.pmid: 20179781
references.citation: Biomaterials. 2011 Oct;32(30):7532-42
references.pmid: 21774980
references.citation: J Clin Periodontol. 2005 Jan;32(1):104-10
references.pmid: 15642067
references.citation: J Biomed Mater Res B Appl Biomater. 2008 Oct;87(1):213-21
references.pmid: 18431753
references.citation: Science. 1993 May 14;260(5110):920-6
references.pmid: 8493529
references.citation: J Artif Organs. 2005;8(3):131-6
references.pmid: 16235028
references.citation: J Microencapsul. 2011;28(5):344-52
references.pmid: 21736519
references.citation: Integr Biol (Camb). 2009 Mar;1(3):252-9
references.pmid: 20023736
references.citation: J Biomed Mater Res. 1996 Dec;32(4):505-12
references.pmid: 8953139
references.citation: Bone. 2012 Apr;50(4):930-41
references.pmid: 22270057
references.citation: Exp Cell Res. 2008 Apr 15;314(7):1575-84
references.pmid: 18325494
references.citation: Biotechnol Lett. 2011 Jun;33(6):1257-64
references.pmid: 21287233