title: Analyzing the meiotic transcriptome using isolated meiocytes of Arabidopsis thaliana.
abstract: Improved transcriptome sequencing technologies (RNA-seq) have advanced our understanding of the tissue-specific transcriptome landscapes, including those of messenger RNAs, noncoding RNAs and small RNAs. However, transcriptome profiles of plant meiocytes remain challenging due to the lack of efficient methods to enrich meiocytes for the analysis of temporal and spatial gene expression patterns during meiosis. In this chapter, we describe a method to analyze the Arabidopsis meiotic transcriptome using isolated male meiocytes.
authors.affiliation: Department of Horticulture, University of Minnesota, St. Paul, MN, USA.
authors.forename: Changbin
authors.initials: C
authors.lastname: Chen
authors.forename: Ernest F
authors.initials: EF
authors.lastname: Retzel
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics*; D019295:Computational Biology; D035264:Flowers / Q000166:cytology / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D023281:Genomics / Q000379:methods; D055993:Germ Cells, Plant / Q000378:metabolism*; D008540:Meiosis / Q000235:genetics*; D018749:RNA, Plant; D017423:Sequence Analysis, RNA
chemical_list: D018749:RNA, Plant