title: Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env.
abstract: No effective vaccine has been developed against the subgroup J avian leukosis virus (ALV-J). The genetic diversity of ALV-J might be related to the env gene, therefore, we selected conserved sequences of the env gene and designed interference sequence. In this study, microRNAs (miRNAs) were designed and synthesized, corresponding to conserved regions of the env gene. These miRNAs were cloned into the linearized eukaryotic expression vector. The recombinant plasmids were transfected into DF-1 cells. After transfection, the cells were inoculated with ALV-J. In reporter assays, the transfection efficiency is 80% by indirect immunofluorescence (IFA). Expression of the virus envelope glycoprotein was measured by IFA and western blotting assays. The relative expression of env gene was determined using quantitative PCR. Our results show that the mi-env 231 and mi-env 1384 could effectively suppress the replication of ALV-J with an efficiency of 68.7-75.2%. These data suggest that the miRNAs targeting the env can inhibit replication of ALV-J efficiently. This finding provides evidence that miRNAs could be used as a potential tool against ALV infection.
authors.affiliation: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nangang District, Harbin 150001, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zai-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Tian
authors.forename: Zhi-Guang
authors.initials: ZG
authors.lastname: Xiao
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Meng
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000628:therapy / Q000821:virology*; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D002645:Chickens; D015316:Genetic Therapy / Q000662:veterinary; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011201:Poultry Diseases / Q000628:therapy / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D035683:MicroRNAs; D012367:RNA, Viral; D014759:Viral Envelope Proteins
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