title: A simple method of mouse lung intubation.
abstract: A simple procedure to intubate mice for pulmonary function measurements would have several advantages in longitudinal studies with limited numbers or expensive animal. One of the reasons that this is not done more routinely is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper we demonstrate a procedure that eliminates one of the major hurdles associated with this intubation, that of visualizing the trachea during the entire time of intubation. The approach uses a 0.5 mm fiberoptic light source that serves as an introducer to direct the intubation cannula into the mouse trachea. We show that it is possible to use this procedure to measure lung mechanics in individual mice over a time course of at least several weeks. The technique can be set up with relatively little expense and expertise, and it can be routinely accomplished with relatively little training. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice, thereby minimizing the number of mice needed and increasing the statistical power by using each mouse as its own control.
authors.affiliation: Department of Environmental Health Sciences, Program in Respiratory Biology and Lung Disease, Johns Hopkins Bloomberg School of Public Health.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Das
authors.forename: Kelvin
authors.initials: K
authors.lastname: MacDonald
authors.forename: Herng-Yu Sucie
authors.initials: HY
authors.lastname: Chang
authors.forename: Wayne
authors.initials: W
authors.lastname: Mitzner
mesh_terms: D000818:Animals; D005336:Fiber Optic Technology; D007442:Intubation, Intratracheal / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014132:Trachea / Q000033:anatomy & histology
references.citation: J Appl Physiol (1985). 1999 Dec;87(6):2362-5
references.pmid: 10601190
references.citation: Lab Anim (NY). 2006 Mar;35(3):39-42
references.pmid: 16505825
references.citation: Lab Anim. 2008 Apr;42(2):222-30
references.pmid: 18435880
references.citation: J Appl Physiol (1985). 2009 Mar;106(3):984-7
references.pmid: 19150857
references.citation: Contemp Top Lab Anim Sci. 2005 Mar;44(2):52-5
references.pmid: 15773778
references.citation: Lab Anim. 2007 Jan;41(1):128-35
references.pmid: 17234059
references.citation: Contemp Top Lab Anim Sci. 1999 Nov;38(6):33-35
references.pmid: 12086445
references.citation: J Appl Physiol (1985). 1995 Aug;79(2):560-6
references.pmid: 7592218
references.citation: Eur Rev Med Pharmacol Sci. 2004 May-Jun;8(3):103-6
references.pmid: 15368792
references.citation: Physiol Genomics. 2000 Jan 24;2(1):29-36
references.pmid: 11015579
references.citation: Lab Anim. 2003 Jul;37(3):204-6
references.pmid: 12869282