title: Prevalence and incidence of latent tuberculosis infection in georgian healthcare workers.
abstract: BACKGROUND
  Tuberculosis is a major occupational hazard in low and middle-income countries. Limited data exist on serial testing of healthcare workers (HCWs) with interferon-γ release assays (IGRAs) for latent tuberculosis infection (LTBI), especially in low and middle-income countries. We sought to evaluate the rates of and risk factors for LTBI prevalence and LTBI test conversion among HCWs using the tuberculin skin test (TST) and QuantiFERON-TB Gold In-tube assay (QFT-GIT).
  
  
  METHODS
  A prospective longitudinal study was conducted among HCWs in the country of Georgia. Subjects completed a questionnaire, and TST and QFT-GIT tests were performed. LTBI testing was repeated 6-26 months after baseline testing.
  
  
  RESULTS
  Among 319 HCWs enrolled, 89% reported prior BCG vaccination, and 60% worked in TB healthcare facilities (HCFs). HCWs from TB HCFs had higher prevalence of positive QFT-GIT and TST than those from non-TB HCFs: 107/194 (55%) vs. 30/125 (31%) QFT-GIT positive (p<0.0001) and 128/189 (69%) vs. 64/119 (54%) TST positive (p = 0.01). There was fair agreement between TST and QFT-GIT (kappa = 0.42, 95% CI 0.31-0.52). In multivariate analysis, frequent contact with TB patients was associated with increased risk of positive QFT-GIT (aOR 3.04, 95% CI 1.79-5.14) but not positive TST. Increasing age was associated with increased risk of positive QFT-GIT (aOR 1.05, 95% CI 1.01-1.09) and TST (aOR 1.05, 95% CI 1.01-1.10). High rates of HCW conversion were seen: the QFT-GIT conversion rate was 22.8/100 person-years, and TST conversion rate was 17.1/100 person-years. In multivariate analysis, female HCWs had decreased risk of TST conversion (aOR 0.05, 95% CI 0.01-0.43), and older HCWs had increased risk of QFT-GIT conversion (aOR 1.07 per year, 95% CI 1.01-1.13).
  
  
  CONCLUSIONS
  LTBI prevalence and LTBI test conversion rates were high among Georgian HCWs, especially among those working at TB HCFs. These data highlight the need for increased implementation of TB infection control measures.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Whitaker
authors.affiliation: Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, United States of America. whitaker.jennifer@mayo.edu
authors.forename: Jennifer
authors.initials: J
authors.lastname: A Whitaker
authors.forename: Veriko
authors.initials: V
authors.lastname: Mirtskhulava
authors.forename: Maia
authors.initials: M
authors.lastname: Kipiani
authors.forename: Drew A
authors.initials: DA
authors.lastname: Harris
authors.forename: Nino
authors.initials: N
authors.lastname: Tabagari
authors.forename: Russell R
authors.initials: RR
authors.lastname: Kempker
authors.forename: Henry M
authors.initials: HM
authors.lastname: Blumberg
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D005846:Georgia (Republic) / Q000453:epidemiology; D006282:Health Personnel; D006801:Humans; D015994:Incidence; D059425:Interferon-gamma Release Tests; D055985:Latent Tuberculosis / Q000453:epidemiology*; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D011446:Prospective Studies; D012307:Risk Factors; D014374:Tuberculin Test; D055815:Young Adult
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