title: Lineage depletion of stromal vascular fractions isolated from human adipose tissue: a novel approach towards cell enrichment technology.
abstract: The therapeutic rationale for tissue repair and regeneration using stem cells is at its infancy and needs advancement in understanding the role of individual component's innate capability. As stem cells of adipose tissue reside in a more heterogeneous population of stromal vascular fractions, cell separation or sorting becomes an eminent step towards revealing their unique properties. This study elucidates the comparative efficacy of lineage depleted adipose derived stromal vascular fraction (SVF) and their innate ability using magnetic activated cell sorter (MACS). To this end, isolated SVF from human adipose tissue was lineage depleted according to the manufacturer's instructions using specific antibody cocktail through MACS. The enriched lineage negative (lin-) and lineage positive (lin+) cell fractions were cultured, phenotypically characterized for the panel of cell surface markers using flowcytometry and subjected to osteoblastic and adipogenic differentiation. The expression profile obtained for lin- cells was CD34-/CD45-/HLADR-/CD49d-/CD140b-/CD31-/CD90+/CD105+/CD73+/CD54+/CD166+/CD117- when compared to Lin+ cells expressing CD34+/CD45+/HLADR-/CD49d-/CD140b+/CD31-/CD90+/CD105+/CD73+/CD54+/CD166+/CD117+ (CD-cluster of differentiation). These results, thus, advances our understanding on the inherent property of the individual cell population. Furthermore, both the fractions exhibited mesodermal lineage differentiation capacity. To conclude, this research pursuit rationalized the regenerative therapeutic applicability of both lin- and lin+ cultures of human adipose tissue for disorders of mesodermal, haematological and vascular origin.
authors.affiliation: Department of Stem Cells, Lifeline Multispeciality Hospital, Chennai, 600 096, India.
authors.forename: S
authors.initials: S
authors.lastname: Indumathi
authors.forename: Rashmi
authors.initials: R
authors.lastname: Mishra
authors.forename: R
authors.initials: R
authors.lastname: Harikrishnan
authors.forename: J S
authors.initials: JS
authors.lastname: Rajkumar
authors.forename: Neha
authors.initials: N
authors.lastname: Kantawala
authors.forename: M
authors.initials: M
authors.lastname: Dhanasekaran
references.citation: Stem Cells. 2006 May;24(5):1294-301
references.pmid: 16410387
references.citation: PLoS One. 2012;7(3):e32481
references.pmid: 22412878
references.citation: Cytometry. 1990;11(2):231-8
references.pmid: 1690625
references.citation: Tissue Eng. 2001 Apr;7(2):211-28
references.pmid: 11304456
references.citation: Cytometry A. 2005 May;65(1):4-14
references.pmid: 15810015
references.citation: Cytotechnology. 2012 Feb 1;:
references.pmid: 22294516
references.citation: Clin Pharmacol Ther. 2007 Sep;82(3):252-64
references.pmid: 17671448
references.citation: Cytometry A. 2010 Jan;77(1):22-30
references.pmid: 19852056
references.citation: Mol Biol Cell. 2002 Dec;13(12):4279-95
references.pmid: 12475952
references.citation: Tissue Eng Part C Methods. 2013 Jan;19(1):1-14
references.pmid: 22681647
references.citation: Exp Cell Res. 2006 Apr 1;312(6):727-36
references.pmid: 16386732
references.citation: J Craniomaxillofac Surg. 2004 Dec;32(6):370-3
references.pmid: 15555520
references.citation: Cell Biochem Funct. 2008 Aug;26(6):664-75
references.pmid: 18636461
references.citation: Cytometry B Clin Cytom. 2009 Jul;76(4):285-90
references.pmid: 19363787
references.citation: Expert Opin Biol Ther. 2003 Aug;3(5):705-13
references.pmid: 12880371
references.citation: Expert Opin Biol Ther. 2007 Feb;7(2):173-84
references.pmid: 17250456
references.citation: Gynecol Oncol. 2005 Aug;98(2):211-6
references.pmid: 15967487
references.citation: Circ Res. 2007 May 11;100(9):1249-60
references.pmid: 17495232
references.citation: Cytotechnology. 2013 Mar;65(2):187-97
references.pmid: 22729554
references.citation: Anal Bioanal Chem. 2010 Aug;397(8):3249-67
references.pmid: 20419490
references.citation: Biol Cell. 2008 Apr;100(4):231-41
references.pmid: 17997718