title: Evidence for exposure-induced DNA repair abnormality is indicative of health and genetic risk.
abstract: A recent focus has been targeted toward the development of functional biomarkers that can be used to predict disease more reliably. One such biomarker is the challenge assay for DNA repair deficiency. Briefly, the assay involves challenging lymphocytes in culture to a DNA damaging agent in vitro and determining the repair outcome in chromosome aberrations and/or DNA strand breaks. The aim is to show that individuals who have chronic exposure to toxic substances will develop exposure-induced DNA repair deficiencies. Many studies around the world have shown that the assay detects DNA repair deficiency in environmentally/occupationally exposed populations and with significant exposure dose-response relationship. The prediction of health risk was also validated. In addition, exposure-induced repair deficiency which was apparently passed through the germ cells had caused genetic consequences in a 3-generation population. The assay is simple to conduct and is more sensitive than some traditional biomarker assays. Together with the functional significance of the assay, the challenge assay can be used with confidence in population studies for health risk assessment.
authors.affiliation: Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Mathuros
authors.initials: M
authors.lastname: Ruchirawat
authors.forename: Antonina
authors.initials: A
authors.lastname: Cebulska-Wasilewska
authors.forename: William W
authors.initials: WW
authors.lastname: Au
mesh_terms: D015415:Biomarkers; D049914:DNA Repair-Deficiency Disorders / Q000139:chemically induced*; D004781:Environmental Exposure / Q000009:adverse effects*; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D009369:Neoplasms; D018570:Risk Assessment
chemical_list: D015415:Biomarkers; D009153:Mutagens
keywords: Challenge assay; DNA repair; Environmental toxicology; Genetic toxicology; Health risk; Population monitoring