title: Stripe rust resistance genes in the UK winter wheat cultivar Claire.
abstract: Stripe rust resistance in the winter wheat cultivar Claire had remained effective in the UK and Europe since its release in 1999 and consequently has been used extensively in wheat breeding programs. However, in 2012, reports indicated that this valuable resistance may now have been compromised. To characterise stripe rust resistance in Claire and determine which genes may still confer effective resistance a cross was made between Claire and the stripe rust susceptible cultivar Lemhi. A genetic linkage map, constructed using SSR, AFLP, DArT and NBS-AFLP markers had a total map length of 1,730 cM. To improve the definition of two quantitative trait loci (QTL) identified on the long arm of chromosome 2D further markers were developed from wheat EST. Stripe rust resistance was evaluated on adult plants under field and glasshouse conditions by measuring the extent of fungal growth and sporulation, percentage infection (Pi) and the necrotic/chlorotic responses of the plant to infection, infection type (IT). Four QTL contributing to stripe rust adult plant resistance (APR) were identified in Claire, QYr.niab-2D.1, QYr.niab-2D.2, QYr.niab-2B and QYr.niab-7B. For Pi QYr.niab-2D.1 explained up to 25.4 % of the phenotypic variation, QYr.niab-2D.2 up to 28.7 %, QYr.niab-2B up to 21.7 % and QYr.niab-7B up to 13.0 %. For IT the percentages of phenotypic variation explained were 23.4, 31.8, 17.2 and 12.6 %, respectively. In addition to the four QTL conferring APR in Claire, a race-specific, seedling expressed resistance gene was identified on chromosome 3B.
authors.affiliation: CSIRO, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia. nicola.powell@csiro.au
authors.forename: N M
authors.initials: NM
authors.lastname: Powell
authors.forename: C M
authors.initials: CM
authors.lastname: Lewis
authors.forename: S T
authors.initials: ST
authors.lastname: Berry
authors.forename: R
authors.initials: R
authors.lastname: Maccormack
authors.forename: L A
authors.initials: LA
authors.lastname: Boyd
mesh_terms: D000383:Agriculture / Q000379:methods; D001487:Basidiomycota; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D040641:Quantitative Trait Loci / Q000235:genetics*; D014908:Triticum / Q000235:genetics*; D006113:United Kingdom
chemical_list: D005819:Genetic Markers
references.citation: Theor Appl Genet. 2008 May;116(8):1155-66
references.pmid: 18347772
references.citation: Theor Appl Genet. 1988 Sep;76(3):393-7
references.pmid: 24232203
references.citation: Genetics. 1989 Jan;121(1):185-99
references.pmid: 2563713
references.citation: Biotechniques. 1996 May;20(5):758-60
references.pmid: 8723911
references.citation: Genome. 2000 Aug;43(4):689-97
references.pmid: 10984182
references.citation: Phytopathology. 1999 Jun;89(6):518-21
references.pmid: 18944724
references.citation: Mol Plant. 2010 Jan;3(1):21-53
references.pmid: 20038549
references.citation: Theor Appl Genet. 2004 May;108(7):1426-33
references.pmid: 14963651
references.citation: Theor Appl Genet. 2011 Mar;122(4):723-33
references.pmid: 21076811
references.citation: Theor Appl Genet. 2002 May;104(6-7):1164-1172
references.pmid: 12582627
references.citation: Genome. 2011 May;54(5):419-30
references.pmid: 21534722
references.citation: Theor Appl Genet. 2004 Jul;109(2):425-32
references.pmid: 15138688
references.citation: Theor Appl Genet. 2006 Feb;112(4):581-91
references.pmid: 16395570
references.citation: Theor Appl Genet. 2005 May;110(8):1401-9
references.pmid: 15841362
references.citation: Theor Appl Genet. 2007 May;114(7):1277-87
references.pmid: 17318493
references.citation: Nucleic Acids Res. 1995 Nov 11;23(21):4407-14
references.pmid: 7501463
references.citation: Theor Appl Genet. 2004 Jul;109(2):384-93
references.pmid: 15057419
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: Theor Appl Genet. 2004 Oct;109(6):1105-14
references.pmid: 15490101
references.citation: Theor Appl Genet. 2005 Feb;110(3):550-60
references.pmid: 15655666
references.citation: Genetics. 1994 Apr;136(4):1447-55
references.pmid: 8013917
references.citation: Theor Appl Genet. 2012 May;124(7):1283-94
references.pmid: 22274764
references.citation: Genome. 2008 Nov;51(11):922-7
references.pmid: 18956025
references.citation: Theor Appl Genet. 2012 Jun;125(1):109-20
references.pmid: 22350093
references.citation: Theor Appl Genet. 2008 Aug;117(3):391-9
references.pmid: 18481042
references.citation: Genetics. 1994 Nov;138(3):963-71
references.pmid: 7851788
references.citation: Theor Appl Genet. 2008 May;116(7):1027-34
references.pmid: 18335201
references.citation: Theor Appl Genet. 2002 Jan;104(1):111-8
references.pmid: 12579435