title: Conversion of estrone to 17 beta-estradiol in Jurkat acute T cell leukemia Hut-78 T- and Raji B lymphoma cell lines in vitro.
abstract: Leukemia and lymphoma cells are positive for estrogen receptors and contain endogenous estrogens and estrogen metabolites. Therefore, we studied the levels of 17 beta-hydroxysteroid-dehydrogenase type 1 (HSD17B1) transcript and protein and the production of 17 beta-estradiol (E2) from estrone (E1) in human Jurkat acute T cell leukemia, Hut-78 and Raji B lymphoma cells. We found the presence of HSD17B1 transcripts and proteins in these cells. Moreover, we demonstrated that Jurkat, Hut-78, and Raji B cells are able to produce E2 from E1. Our results indicate that Jurkat acute T cell leukemia, Hut-78 and Raji B lymphoma cells are able to convert weak estrogen E1 to the more potent E2 in vitro.
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, 6 Święcickiego Street, 60781 Poznań, Poland.
authors.forename: Aleksander J
authors.initials: AJ
authors.lastname: Strugała
authors.forename: Paweł P
authors.initials: PP
authors.lastname: Jagodziński
mesh_terms: D045744:Cell Line, Tumor; D004958:Estradiol / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000235:genetics*; D004970:Estrone / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000378:metabolism*; D016399:Lymphoma, T-Cell / Q000378:metabolism*
chemical_list: D004970:Estrone; D004958:Estradiol; D004960:Estradiol Dehydrogenases; C513331:HSD17B1 protein, human