title: Preparation of Eudragit L 100-55 enteric nanoparticles by a novel emulsion diffusion method.
abstract: In this study, a novel emulsion diffusion method was used to prepare enteric Eudragit L100-55 nanoparticles by ultrasonic dispersion and diffusion solidification. Omeprazole was selected as the model drug. The prepared nanoparticles were in spherical shape and exhibited negative zeta potential. The Fourier transform infrared spectroscopy results indicated that no molecular interaction occurred between the drug molecule and polymer chain. In addition, the nanoparticles showed a strong pH-sensitive release in vitro. A mild cytotoxicity of nanoparticle was observed in the subsequent studies, and the particle cellular uptake study showed that the nanoparticles could be taken up by Caco-2 cells after 0.5h incubation. Our results indicated that the enteric Eudragit L 100-55 nanoparticle could be synthesized successfully via this ultrasonic solidification method, which also could be applied to prepare other pH-sensitive polymer nanoparticles.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Hao
authors.forename: Bochu
authors.initials: B
authors.lastname: Wang
authors.forename: Yazhou
authors.initials: Y
authors.lastname: Wang
authors.forename: Liancai
authors.initials: L
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Tingwang
authors.initials: T
authors.lastname: Guo
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000494:pharmacology; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000494:pharmacology; D001692:Biological Transport; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000494:pharmacology; D004058:Diffusion; D004655:Emulsions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D009853:Omeprazole / Q000737:chemistry / Q000494:pharmacology; D010316:Particle Size; D013010:Sonication; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000180:Acrylic Resins; D000897:Anti-Ulcer Agents; D003692:Delayed-Action Preparations; D004655:Emulsions; C446821:Eudragit L100-55; D009853:Omeprazole