title: Penetration of nanoparticles in flax (Linum usitatissimum L.) calli and regenerants.
abstract: We demonstrate a method for direct delivery of metal nanoparticles to flax calli and regenerant cells by vacuum deposition of metal nanolayers on powdered hormone followed by dispersal of the combined hormone-metal in medium. The penetration and location of the gold (AuNPs) and silver (AgNPs) nanoparticles in calli and in plant regenerants were confirmed by optical absorption spectroscopy and scanning electron microscopy. We detected a significant effect of the AuNPs and AgNPs on the regeneration type of flax calli.
authors.affiliation: Institute of Systematic Biology, Daugavpils University, Vienības 13, Daugavpils LV-5401, Latvia. inese.kokina@biology.lv
authors.forename: Inese
authors.initials: I
authors.lastname: Kokina
authors.forename: Vjačeslavs
authors.initials: V
authors.lastname: Gerbreders
authors.forename: Eriks
authors.initials: E
authors.lastname: Sledevskis
authors.forename: Andrejs
authors.initials: A
authors.lastname: Bulanovs
mesh_terms: D019597:Flax / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D006046:Gold