title: An ethanolamine-phosphate modified glycolipid in Clostridium acetobutylicum that responds to membrane stress.
abstract: Two phosphorus-containing glycolipids have previously been observed in Clostridium acetobutylicum. We had shown that the concentration of one of them increases in response to increased unsaturation of the membrane lipid hydrocarbon chains, suggesting a potential role in the regulation of lipid polymorphism in this organism. Mass spectrometry shows that these glycolipids are ethanolamine phosphate (Etn-P)-containing derivatives of a mono- and di-glycosyldiradylglycerol. The content of both diglycosyldiradylglycerol and the Etn-P-monoglycosyldiradylglycerol, which increases upon increased unsaturation of the membrane, also increases upon addition of octanol to the medium. Thus, it appears that the Etn-P-monoglycosyldiradylglycerol along with the diglycosyldiradylglycerol may serve to stabilize the membrane bilayer during membrane stress caused by the presence of the solvents produced during fermentation.
authors.affiliation: Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Tian
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Guan
authors.forename: Howard
authors.initials: H
authors.lastname: Goldfine
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D002853:Chromatography, Liquid; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism*; D005285:Fermentation; D006017:Glycolipids / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013312:Stress, Physiological
chemical_list: D004983:Ethanolamines; D006017:Glycolipids; D008563:Membrane Lipids; D012997:Solvents; C005448:phosphorylethanolamine
references.citation: Microbiology (Reading). 2012 Oct;158(Pt 10):2577-2584
references.pmid: 22837302
references.citation: Can J Biochem Physiol. 1959 Aug;37(8):911-7
references.pmid: 13671378
references.citation: J Bacteriol. 1976 Feb;125(2):670-8
references.pmid: 1107328
references.citation: Appl Environ Microbiol. 1987 Dec;53(12):2854-61
references.pmid: 16347502
references.citation: Biochemistry. 1995 Oct 24;34(42):13811-7
references.pmid: 7577974
references.citation: Microbiol Rev. 1986 Dec;50(4):484-524
references.pmid: 3540574
references.citation: Biochim Biophys Acta. 1977 Apr 18;466(2):336-46
references.pmid: 870042
references.citation: Appl Environ Microbiol. 1985 Nov;50(5):1165-70
references.pmid: 2868690
references.citation: Appl Environ Microbiol. 1984 Jan;47(1):193-4
references.pmid: 6696415
references.citation: Biochim Biophys Acta. 1985 Feb 28;813(1):10-8
references.pmid: 3970912
references.citation: Biochemistry. 1987 May 19;26(10):2814-22
references.pmid: 3606994
references.citation: Biochemistry. 1980 Aug 5;19(16):3650-5
references.pmid: 7407064
references.citation: J Lipid Res. 2010 Jul;51(7):1953-61
references.pmid: 20173213
references.citation: Biochim Biophys Acta. 2011 Mar;1811(3):186-93
references.pmid: 21195206
references.citation: Biochemistry. 1986 Nov 18;25(23):7511-7
references.pmid: 3801430
references.citation: Appl Environ Microbiol. 1991 Dec;57(12):3517-21
references.pmid: 1785927
references.citation: J Bacteriol. 1992 Mar;174(6):1848-53
references.pmid: 1548233
references.citation: Biochemistry. 1995 Oct 24;34(42):13818-24
references.pmid: 7577975