title: Follow-up study of abnormal biological indicators and gene expression in the peripheral blood of three accidentally exposed persons.
abstract: In order to identify biomarkers for early diagnosis and/or for therapeutic targets in the delayed health effects of ionizing radiation, we analyzed the subgroups of lymphocytes, serum protein levels and gene expression profiles in the peripheral blood of three ⁶⁰Co γ-ray accidentally exposed persons during the three years after irradiation. Flow cytometry analyses and agarose gel electrophoresis were applied to investigate the subgroups of lymphocytes and the composition of serum proteins, respectively. Gene expression profiling was obtained using a whole genome gene expression chip assay. Both the percentage of CD4+ T lymphocytes and the ratio of Th to Ts were reduced compared with the normal control values. The percentage of albumin decreased whereas beta globulin increased. There were 285 up-regulated and 446 down-regulated genes in irradiated samples relative to the control samples. The expression of KDR, CEACAM8 and OSM was validated by RT-PCR. The majority of the differentially expressed genes encode proteins associated with the immune response, inflammation, oncogenesis, cell structure, oxidative stress, neuro-hormone regulation, reproduction, susceptibility to psychiatric disorders, or transcriptional regulation. We have identified a number of promising novel candidates that have potential for serving as biomarkers for delayed damage. Furthermore, the changes in the immunological indicator CD4+ T cells, and the ratio of CD4+ T to CD8+ T cells may be biomarkers for the prediction of delayed damage by ionizing radiation. The findings of our study are useful for forming a comprehensive understanding of the mechanisms underlying the delayed effects of ionizing radiation.
authors.affiliation: Department of Radiation Medicine and Environmental Medicine, China Institute for Radiation Protection, Taiyuan, 030006, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Chi
authors.forename: Rong
authors.initials: R
authors.lastname: Tian
authors.forename: Huifang
authors.initials: H
authors.lastname: Liu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Jinping
authors.initials: J
authors.lastname: Wei
authors.forename: Jianping
authors.initials: J
authors.lastname: Guo
authors.forename: Fengling
authors.initials: F
authors.lastname: Guo
authors.forename: Shufang
authors.initials: S
authors.lastname: Li
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011829:Radiation Dosage; D018788:Radioactive Hazard Release
chemical_list: D001798:Blood Proteins
keywords: accidental exposure; gene expression profile; radiation biomarkers; ratio of Th to Ts
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