title: Amphiphilic N-(2,3-dihydroxypropyl)-chitosan-cholic acid micelles for paclitaxel delivery.
abstract: Self-assembled amphiphilic N-(2,3-dihydroxypropyl)-chitosan-cholic acid (DHP-CS-CHO) micelle was prepared as a carrier for paclitaxel. DHP-CS-CHO was synthesized by grafted small molecules cholic acid and glycidol onto primary amine group of chitosan, respectively. The DHP-CS-CHO formed uniform micelles (size=212.4±3.1 nm) with a low critical micelle concentration (0.024 mg/ml) in PBS. Hydrophobic anticancer drug, paclitaxel (PTX), was easily encapsulated into chitosan derivative micelles by a dialysis method with loading efficiency up to 80%. The PTX loaded micelles released the drug in a sustained manner more than a week in PBS containing 0.1% (w/v) Tween 80 at 37°C. In vitro antitumor experiment demonstrated that PTX loaded chitosan derivative micelles could inhibit MCF-7 cell growth and induce its apoptosis. These results suggested that DHP-CS-CHO may be a promising carrier for the anticancer drug PTX.
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunication, Chongqing, China. letter2012@yahoo.cn
authors.forename: Zheng
authors.initials: Z
authors.lastname: Pan
authors.forename: Yunling
authors.initials: Y
authors.lastname: Gao
authors.forename: Linseng
authors.initials: L
authors.lastname: Heng
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Gan
authors.initials: G
authors.lastname: Yao
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D019826:Cholic Acid / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D061986:MCF-7 Cells; D008823:Micelles; D017239:Paclitaxel / Q000737:chemistry*; D010316:Particle Size; D013501:Surface-Active Agents
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008823:Micelles; D013501:Surface-Active Agents; D048271:Chitosan; D019826:Cholic Acid; D017239:Paclitaxel