title: Supplementation of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 in diet-induced obese mice is associated with gut microbial changes and reduction in obesity.
abstract: OBJECTIVE
  To investigate the functional effects of probiotic treatment on the gut microbiota, as well as liver and adipose gene expression in diet-induced obese mice.
  
  
  METHODS
  Male C57BL/6J mice were fed a high-fat diet (HFD) for 8 weeks to induce obesity, and then randomized to receive HFD+probiotic (Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032, n = 9) or HFD+placebo (n = 9) for another 10 weeks. Normal diet (ND) fed mice (n = 9) served as non-obese controls.
  
  
  RESULTS
  Diet-induced obese mice treated with probiotics showed reduced body weight gain and fat accumulation as well as lowered plasma insulin, leptin, total-cholesterol and liver toxicity biomarkers. A total of 151,061 pyrosequencing reads for fecal microbiota were analyzed with a mean of 6,564, 5,274 and 4,464 reads for the ND, HFD+placebo and HFD+probiotic groups, respectively. Gut microbiota species were shared among the experimental groups despite the different diets and treatments. The diversity of the gut microbiota and its composition were significantly altered in the diet-induced obese mice and after probiotic treatment. We observed concurrent transcriptional changes in adipose tissue and the liver. In adipose tissue, pro-inflammatory genes (TNFα, IL6, IL1β and MCP1) were down-regulated in mice receiving probiotic treatment. In the liver, fatty acid oxidation-related genes (PGC1α, CPT1, CPT2 and ACOX1) were up-regulated in mice receiving probiotic treatment.
  
  
  CONCLUSIONS
  The gut microbiota of diet-induced obese mice appears to be modulated in mice receiving probiotic treatment. Probiotic treatment might reduce diet-induced obesity and modulate genes associated with metabolism and inflammation in the liver and adipose tissue.
authors.affiliation: Korea Yakult Co., Ltd., Yongin, Gyeonggi, Republic of Korea.
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Park
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
authors.forename: Se-Hoon
authors.initials: SH
authors.lastname: Park
authors.forename: Chul-Sung
authors.initials: CS
authors.lastname: Huh
authors.forename: Sae-Rom
authors.initials: SR
authors.lastname: Yoo
authors.forename: Rina
authors.initials: R
authors.lastname: Yu
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Sung
authors.forename: Robin A
authors.initials: RA
authors.lastname: McGregor
authors.forename: Myung-Sook
authors.initials: MS
authors.lastname: Choi
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000818:Animals; D044822:Biodiversity; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D004032:Diet / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006728:Hormones / Q000097:blood; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D054892:Metagenome / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000382:microbiology*; D019936:Probiotics / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D006728:Hormones; D002784:Cholesterol
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