title: Axonal translation of β-catenin regulates synaptic vesicle dynamics.
abstract: Many presynaptic transcripts have been observed in axons, yet their role in synapse development remains unknown. Using visually and pharmacologically isolated presynaptic terminals from dissociated rat hippocampal neurons, we found that ribosomes and β-catenin mRNA preferentially localize to recently formed boutons. Locally translated β-catenin accumulates at presynaptic terminals, where it regulates synaptic vesicle release dynamics. Thus, local translation of β-catenin is a newly described mechanism for axons to independently functionalize nerve terminals at great distances from cellular somata.
authors.affiliation: University of North Carolina at Chapel Hill, Joint Department of Biomedical Engineering, Chapel Hill, North Carolina 27599, USA. amtaylor@unc.edu
authors.forename: Anne Marion
authors.initials: AM
authors.lastname: Taylor
authors.forename: Jason
authors.initials: J
authors.lastname: Wu
authors.forename: Hwan-Ching
authors.initials: HC
authors.lastname: Tai
authors.forename: Erin M
authors.initials: EM
authors.lastname: Schuman
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D003505:Cyclodextrins / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D015194:Diffusion Chambers, Culture; D004622:Embryo, Mammalian; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D006624:Hippocampus / Q000166:cytology; D008297:Male; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011726:Pyridinium Compounds / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D018709:Statistics, Nonparametric; D013572:Synaptic Vesicles / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology; D051176:beta Catenin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: C403046:ADVASEP 7; C113688:Bsn protein, rat; D003505:Cyclodextrins; D018691:Excitatory Amino Acid Antagonists; C508379:N-(3-trimethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium; D009419:Nerve Tissue Proteins; D011500:Protein Synthesis Inhibitors; D011726:Pyridinium Compounds; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D034741:RNA, Small Interfering; D014404:Tubulin; D051176:beta Catenin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; C056639:2-amino-5-phosphopentanoic acid; D003513:Cycloheximide; D014633:Valine
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