title: Trypanocidal activity of the essential oils in their conventional and nanoemulsion forms: in vitro tests.
abstract: The aim of this study was to investigate the susceptibility in vitro of Trypanosoma evansi to the essential oils of andiroba (Carapa guaianensis) and aroeira (Schinus molle), in their conventional and nanostructured forms. For that, pure oils at concentrations of 0.5%, 1.0% and 2.0% were used. A negative control (untreated) and a positive control (diminazene aceturate 0.5%) were used as comparative parameters. Later, the same tests were performed, using nanoemulsions oils at concentrations of 0.5% and 1.0%. The tests were carried out in triplicates and the numbers of parasites were quantified on 1, 3 and 6 h from onset of the study. A dose-dependent reduction in the number of parasites to the forms of two oils tested was observed after 1 h. The concentration of parasites was significantly reduced at low concentrations after 3 h, as well as at 6 h no alive parasites were observed for the essential oils tested. Ours findings indicate, for the first time, that oils of andiroba and aroeira (in their conventional and nanoemulsion forms) have high activity against T. evansi in vitro, leading to the suggestion that these oils may be applied as an alternative treatment for this disease.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Camila B
authors.initials: CB
authors.lastname: Oliveira
authors.forename: Carine E P
authors.initials: CE
authors.lastname: Zimmermann
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Vaucher
authors.forename: Roberto C V
authors.initials: RC
authors.lastname: Santos
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Rech
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Tonin
authors.forename: Janice L
authors.initials: JL
authors.lastname: Giongo
authors.forename: Cristiane B
authors.initials: CB
authors.lastname: Mattos
authors.forename: Letícia
authors.initials: L
authors.lastname: Koester
authors.forename: Janio M
authors.initials: JM
authors.lastname: Santurio
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D001681:Biological Assay; D003470:Culture Media; D004133:Diminazene / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D029582:Meliaceae / Q000737:chemistry*; D051379:Mice; D049329:Nanostructures; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D009994:Osmolar Concentration; D010936:Plant Extracts / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000737:chemistry / Q000494:pharmacology*; D014345:Trypanosoma / Q000187:drug effects*
chemical_list: D003470:Culture Media; D004655:Emulsions; D009822:Oils, Volatile; D010936:Plant Extracts; D014344:Trypanocidal Agents; C003915:diminazene aceturate; D004133:Diminazene