title: Cadmium tolerance and removal from Cunninghamella elegans related to the polyphosphate metabolism.
abstract: The aim of the present work was to study the cadmium effects on growth, ultrastructure and polyphosphate metabolism, as well as to evaluate the metal removal and accumulation by Cunninghamella elegans (IFM 46109) growing in culture medium. The presence of cadmium reduced growth, and a longer lag phase was observed. However, the phosphate uptake from the culture medium increased 15% when compared to the control. Moreover, C. elegans removed 70%-81% of the cadmium added to the culture medium during its growth. The C. elegans mycelia showed a removal efficiency of 280 mg/g at a cadmium concentration of 22.10 mg/L, and the removal velocity of cadmium was 0.107 mg/h. Additionally, it was observed that cadmium induced vacuolization, the presence of electron dense deposits in vacuoles, cytoplasm and cell membranes, as well as the distinct behavior of polyphosphate fractions. The results obtained with C. elegans suggest that precipitation, vacuolization and polyphosphate fractions were associated to cadmium tolerance, and this species demonstrated a higher potential for bioremediation of heavy metals.
authors.affiliation: Department of Biology, Federal Rural University of Pernambuco, Recife, PE 52171-900, Brazil. galba_takaki@yahoo.com.br.
authors.forename: Marcos A B
authors.initials: MA
authors.lastname: de Lima
authors.forename: Luciana de O
authors.initials: Lde O
authors.lastname: Franco
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: de Souza
authors.forename: Aline E
authors.initials: AE
authors.lastname: do Nascimento
authors.forename: Carlos A A
authors.initials: CA
authors.lastname: da Silva
authors.forename: Rita de C C
authors.initials: Rde C
authors.lastname: Maia
authors.forename: Hercília M L
authors.initials: HM
authors.lastname: Rolim
authors.forename: Galba M C
authors.initials: GM
authors.lastname: Takaki
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002104:Cadmium / Q000302:isolation & purification* / Q000633:toxicity*; D020093:Cunninghamella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D011122:Polyphosphates / Q000378:metabolism*
chemical_list: D011122:Polyphosphates; D002104:Cadmium
references.citation: Biochemistry (Mosc). 2000 Mar;65(3):349-54
references.pmid: 10739478
references.citation: J Biosci Bioeng. 1999;88(2):111-29
references.pmid: 16232585
references.citation: Appl Environ Microbiol. 2004 Sep;70(9):5177-82
references.pmid: 15345397
references.citation: Appl Environ Microbiol. 2000 Sep;66(9):4068-73
references.pmid: 10966430
references.citation: Water Res. 2001 Apr;35(6):1425-34
references.pmid: 11317889
references.citation: Biotechnol Prog. 1995 May-Jun;11(3):235-50
references.pmid: 7619394
references.citation: Biometals. 2006 Dec;19(6):593-9
references.pmid: 16946986
references.citation: Curr Opin Biotechnol. 2001 Jun;12(3):248-53
references.pmid: 11404102
references.citation: Biometals. 2010 Oct;23(5):951-60
references.pmid: 20390439
references.citation: Environ Int. 2004 Apr;30(2):261-78
references.pmid: 14749114
references.citation: Appl Microbiol Biotechnol. 1999 Jun;51(6):730-50
references.pmid: 10422221
references.citation: FEMS Microbiol Ecol. 2003 May 1;44(2):253-9
references.pmid: 19719642
references.citation: Molecules. 2011 Mar 15;16(3):2486-500
references.pmid: 21407149
references.citation: Appl Environ Microbiol. 2004 Dec;70(12):7404-12
references.pmid: 15574942
references.citation: Appl Environ Microbiol. 1984 Feb;47(2):374-7
references.pmid: 6370136
references.citation: Bioresour Technol. 2002 Oct;85(1):103-5
references.pmid: 12146636
references.citation: J Ind Microbiol Biotechnol. 1997 Nov-Dec;19(5-6):324-33
references.pmid: 9451829
references.citation: Bioresour Technol. 2008 Mar;99(5):1339-44
references.pmid: 17376676
references.citation: Appl Environ Microbiol. 1996 Mar;62(3):798-803
references.pmid: 8975609
references.citation: Br Med Bull. 2003;68:167-82
references.pmid: 14757716
references.citation: Int Microbiol. 2000 Mar;3(1):17-24
references.pmid: 10963329
references.citation: Toxicol Appl Pharmacol. 2009 Aug 1;238(3):272-9
references.pmid: 19371617
references.citation: Appl Environ Microbiol. 1993 Jan;59(1):7-14
references.pmid: 8439169
references.citation: Bioresour Technol. 2006 May;97(8):1018-25
references.pmid: 16324839
references.citation: Xenobiotica. 1994 Nov;24(11):1077-83
references.pmid: 7701849
references.citation: Biochemistry (Mosc). 2000 Mar;65(3):324-31
references.pmid: 10739475
references.citation: Environ Geochem Health. 2010 Feb;32(1):1-12
references.pmid: 19688602
references.citation: J Appl Microbiol. 2008 Jan;104(1):178-84
references.pmid: 17850314
references.citation: Bioresour Technol. 2004 Jan;91(1):69-75
references.pmid: 14585623
references.citation: Indian J Med Res. 2008 Oct;128(4):557-64
references.pmid: 19106447
references.citation: Microbiology (Reading). 2006 Jan;152(Pt 1):59-66
references.pmid: 16385115
references.citation: Annu Rev Biochem. 1999;68:89-125
references.pmid: 10872445
references.citation: FEMS Microbiol Lett. 2002 Feb 5;207(2):159-64
references.pmid: 11958934
references.citation: Bioresour Technol. 2002 Feb;81(3):167-70
references.pmid: 11800481