title: Labeling meiotic chromosomes in maize with fluorescence in situ hybridization.
abstract: Fluorescence in situ hybridization (FISH) can be used to visualize chromosomal features using repetitive or single gene probes above a minimum target size. When applied to meiosis, each chromosome of the karyotypic complement can be identified, which can facilitate an understanding of the interrelationship of different chromosomes during this process. On the other hand, the pachytene stage of early meiosis is characterized by slightly but not strongly condensed chromosomes that permit more detailed analyses of adjacent features than can be achieved with somatic metaphase chromosomes.
authors.affiliation: Division of Biological Sciences, University of Missouri-Columbia, Columbia, MO, USA.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Gao
authors.forename: Fangpu
authors.initials: F
authors.lastname: Han
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Danilova
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Lamb
authors.forename: Patrice S
authors.initials: PS
authors.lastname: Albert
authors.forename: James A
authors.initials: JA
authors.lastname: Birchler
mesh_terms: D020223:Chromosome Painting / Q000379:methods*; D032461:Chromosomes, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008540:Meiosis / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods; D003313:Zea mays / Q000235:genetics*