title: Positive contribution of IRE1α-XBP1 pathway to the expression of placental cathepsins.
abstract: IRE1α is an ER-located transmembrane RNase whose activation leads to the production of the transcriptional factor, XBP1. Recently, many studies report that IRE1α-XBP1 pathway has novel and significant roles in placenta. However, its molecular details have been still unknown. To address this point, we have focused on the molecular linkage between IRE1α-XBP1 pathway and Cts7 and Cts8, which are essential cathepsins for placenta formation. In cellular model, this pathway positively contributed to their expression at transcriptional level. In addition, the disruption of IRE1α or XBP1 in animal model significantly attenuated their transcripts in placenta. These results indicated that IRE1α-XBP1 pathway function as a specific program supporting the placenta formation by ensuring the moderate expression of specific subset of placental cathepsins.
authors.affiliation: Iwawaki Lab, Advanced Scientific Research Leaders Development Unit, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. oikawa@showa.gunma-u.ac.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Oikawa
authors.forename: Takao
authors.initials: T
authors.lastname: Iwawaki
mesh_terms: D051702:Activating Transcription Factor 6 / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000166:cytology / Q000201:enzymology*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D056811:Unfolded Protein Response; D000071717:X-Box Binding Protein 1
chemical_list: D051702:Activating Transcription Factor 6; C497112:Atf6 protein, mouse; C410987:Cts7 protein, mouse; C410988:Cts8 protein, mouse; D004268:DNA-Binding Proteins; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605544:Xbp1 protein, mouse; D008156:Luciferases; C510185:Ern1 protein, mouse; D017346:Protein Serine-Threonine Kinases; D004722:Endoribonucleases; D002403:Cathepsins