title: Generation of human-induced pluripotent stem cells by lentiviral transduction.
abstract: Human somatic cells can be reprogrammed to the pluripotent state to become human-induced pluripotent stem cells (hiPSC). This reprogramming is achieved by activating signaling pathways that are expressed during early development. These pathways can be induced by ectopic expression of four transcription factors-Oct4, Sox2, Klf4, and c-Myc. Although there are many ways to deliver these transcription factors into the somatic cells, this chapter will provide protocols that can be used to generate hiPSC from lentiviruses.
authors.affiliation: Rutgers NIMH Stem Cell Center and Department of Genetics, Rutgers, The State University of New Jersey, Nelson Biological Laboratory, Piscataway, NJ, USA.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Moore
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D015925:Cryopreservation; D003470:Culture Media; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D000090062:Kruppel-Like Factor 4; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D003470:Culture Media; C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D011994:Recombinant Proteins; D014157:Transcription Factors