title: Cardiac oxidative damage in mice following exposure to nanoparticulate titanium dioxide.
abstract: Nanoparticulate titanium dioxide (nano-TiO2 ) is a widely used powerful nanoparticulate material with high stability, anticorrosion, and photocatalytic property. However, it is possible that during nano-TiO2 exposure, there may be negative effects on cardiovascular system in intoxicated mice. The present study was therefore undertaken to determine nano-TiO2 -induced oxidative stress and to determine whether nano-TiO2 intoxication alters the antioxidant system in the mouse heart exposed to 2.5, 5, and 10 mg/kg body weight nano-TiO2 for 90 consecutive days. The findings showed that long-term exposure to nano-TiO2 resulted in obvious titanium accumulation in heart, in turn led to sparse cardiac muscle fibers, inflammatory response, cell necrosis, and cardiac biochemical dysfunction. Nano-TiO2 exposure promoted remarkably reactive oxygen species production such as superoxide radicals, hydrogen peroxide, and increased malondialdehyde, carbonyl and 8-OHdG levels as degradation products of lipid, protein, and DNA peroxidation in heart. Furthermore, nano-TiO2 exposure attenuated the activities of antioxidative enzymes, such as superoxide dismutase, ascorbate peroxidase, glutathione reductase, glutathione-S-transferase, and levels of antioxidants including ascorbic acid, glutathione, and thiol in heart. Therefore, TiO2 NPs exposure may impair cardiovascular system in mice, and attention should be aroused on the application of nano-TiO2 and their potential long-term exposure effects especially on human beings.
authors.affiliation: Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sheng
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.forename: Xuezi
authors.initials: X
authors.lastname: Sang
authors.forename: Yuguan
authors.initials: Y
authors.lastname: Ze
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhao
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Suxin
authors.initials: S
authors.lastname: Gui
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cheng
authors.forename: Renping
authors.initials: R
authors.lastname: Hu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D004781:Environmental Exposure; D005260:Female; D019803:Glutathione Disulfide / Q000378:metabolism; D006334:Heart Function Tests; D006801:Humans; D008055:Lipids / Q000737:chemistry; D053768:Metal Nanoparticles / Q000009:adverse effects*; D051379:Mice; D009206:Myocardium / Q000473:pathology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010545:Peroxides / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D014025:Titanium / Q000008:administration & dosage* / Q000032:analysis
chemical_list: D000975:Antioxidants; D004220:Disulfides; D008055:Lipids; D010545:Peroxides; C009495:titanium dioxide; D004247:DNA; D014025:Titanium; D019803:Glutathione Disulfide
keywords: antioxidative defense system; heart; nanoparticulate titanium dioxide; oxidative stress; pathological changes