title: Analysis of the titer and reactivity of antibody/ies against fungal cell wall β-glucans in human sera.
abstract: The aim of this work was to characterize an antibody response to β-glucan (BG), a major component of the fungal cell wall, at each isotype in human sera. The titer and reactivity of the anti-human BG antibody were examined using enzyme-linked immunosorbant assay plates coated with Candida soluble cell wall β-glucan as a standard antigen. The antibody was detected using anti-human immunoglobulin (Ig) G, IgM, and IgA. Its major class was IgG in all subjects. The antibody titer varied significantly. The anti-BG antibody showed greater reactivity to β-glucans derived from pathogenic fungi than monoglycosyl-branched β-glucans derived from mycelia culture medium. In addition, it was suggested that the anti-BG IgM antibody was bound relatively strongly to the β1,3-glucan backbone and the anti-BG IgG antibody to β1,6-glucan. The anti-BG antibody plays a variety of roles, according to class, in the host's response to fungi. We propose a new index of human response to β-glucan that effects the understanding of the response to β-glucan in humans.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Mayu
authors.initials: M
authors.lastname: Morita
authors.forename: Masuro
authors.initials: M
authors.lastname: Motoi
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Noriko N
authors.initials: NN
authors.lastname: Miura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood*; D000918:Antibody Specificity; D001230:Aspergillus / Q000166:cytology* / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D055815:Young Adult; D047071:beta-Glucans / Q000276:immunology*
chemical_list: D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D047071:beta-Glucans