title: Design and implementation of injector/distributor structures for microfabricated non-porous pillar columns for capillary electrochromatography.
abstract: A previously proposed foil definition is applied in the design of injector/distributor structures for solid microfabricated column structures for capillary electrochromatography. In addition to a typical bifurcated distributor, an optimized design alternative with two different configurations is experimentally evaluated. Optimized designs yielded a flat profile for the injected sample with a maximum of 3% variation from the mean width, while it went up to 18% for the typical bifurcated distributor. The implemented electrokinetic injection approach enabled controlling the volume of the injected sample accurately without sacrificing the compactness of the device design. The width of the injected sample was directly proportional to the injection time, namely 165 and 218 μm base widths were obtained for 0.6 and 0.8s of feeding, respectively. Reducing the external porosity of the distributor by 85% compared to the typical design, optimized distributors caused a decrease in the mean flow velocity of up to 70%. However, having a flat initial plug shape enabled the separation of a mixture of Coumarin 440, 460, 480 and 540 at 1 mm downstream of the injection point in 80s, while it was even not possible to detect the C440 signal for a typical bifurcated design.
authors.affiliation: Mesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, PO Box 217, 7500AE Enschede, The Netherlands. s.sukas@utwente.nl
authors.forename: Sertan
authors.initials: S
authors.lastname: Sukas
authors.forename: Gert
authors.initials: G
authors.lastname: Desmet
authors.forename: Han J G E
authors.initials: HJ
authors.lastname: Gardeniers
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation*; D003374:Coumarins / Q000032:analysis*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000032:analysis*; D055616:Microtechnology; D016062:Porosity
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes