title: [Detection of anaerobes and drug sensitivity from the periodontal pockets of patients with combined periodontal-endodontic lesions].
abstract: OBJECTIVE
  To investigate the correlation between the composition of bacterial flora isolated from deep periodontal pockets with combined periodontal-endodontic lesions, and to test the antibiotic susceptibility of anaerobes.
  
  
  METHODS
  Microbial samples were taken from 82 deep periodontal pockets with combined periodontal-endodontic lesions using adequate techniques, and divided into two groups: endo-perio group and perio-endo group. Antibiotic susceptibility of cultivable anaerobes were tested with agar dilution method.
  
  
  RESULTS
  Eighty-six cultivable anaerobes were isolated, including Streptococcus spp, Prevotella spp, Actinomycetes spp, Veillonella spp, Fusobacterium spp and etc. 53.4% were gram-positive anaerobes, while 46.51% were gram-negative anaerobes. The detection rate of anaerobes in endo-perio group and that in perio-endo group had significant difference. Most isolated anaerobes were sensitive to six drugs. The resistance rates of penicillin, clindamycin, metronidazole were 6.8%, 13.9% and 2.2%.
  
  
  CONCLUSIONS
  There is a close relationship between anaerobes and combined periodontal-endodontic lesions. Drug sensitive susceptibility should be detected prior to clinical treatment of combined periodontal-endodontic lesions.
authors.affiliation: Yangpu Dental Clinic, Shanghai 200090, China. zhoukang_dentist@163.com
authors.forename: Kang
authors.initials: K
authors.lastname: Zhou
authors.forename: Pei-hong
authors.initials: PH
authors.lastname: Ji
authors.forename: Li-ying
authors.initials: LY
authors.lastname: Yu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Chen
authors.forename: Quan-lin
authors.initials: QL
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents; D001421:Bacteria, Anaerobic; D002981:Clindamycin; D006801:Humans; D008795:Metronidazole; D010514:Periodontal Pocket; D018720:Prevotella
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole; D002981:Clindamycin