title: Progesterone regulation of Na/K-ATPase β1 subunit expression in the mouse uterus during the peri-implantation period.
abstract: Luminal closure and embryo apposition are essential for blastocyst attachment during early pregnancy. In our preliminary microarray results (unpublished data), sodium-potassium adenosine triphosphatase (Na/K-ATPase) β1 (Atp1b1) was highly expressed in mouse uterus on Days 3 and 4 of pregnancy. However, expression and regulation of Atp1b1 in the mammalian uterus during early pregnancy are unknown. Using in situ hybridization, a strong level of Atp1b1 mRNA was detected in luminal epithelial cells on Days 3 and 4 of pregnancy (Day 1 = day of vaginal plug). The expression pattern of FXYD domain-containing ion transport regulator 4 (Fxyd4) was similar to that of Atp1b1. Real-time reverse transcription polymerase chain reaction confirmed the high expression level of Atp1b1 mRNA. Compared with Day 1, the mRNA level of Atp1b1 on Days 3 and 4 increased by 3.5 ± 0.5 and 4.5 ± 0.5 fold, respectively. When the embryo invaded through epithelial cells into the maternal stromal compartment on day 5, Atp1b1 expression decreased to a basal level. Progesterone stimulated Atp1b1 expression by 2.8 ± 1 fold compared with oil in ovariectomized mice at 24 hours after treatment. Expression of Atp1b1 was further upregulated to 4 ± 0.4 fold by estrogen and progesterone. Based on time-course study, progesterone rapidly induced Atp1b1 expression at 6 and 12 hours (13.7 ± 0.5 and 16.6 ± 1.4, respectively); furthermore, this upregulation was blocked by RU486 (progesterone receptor antagonist). Transcription activity of the Atp1b1 promoter was (Day 1 = day of vaginal plug) stimulated by CCAAT/enhancer binding protein beta (Cebpb). In conclusion, Atp1b1 was highly expressed in luminal epithelium during peri-implantation and upregulated by progesterone.
authors.affiliation: College of Life Science, Xiamen University, Xiamen, China; Department of Biology, Shantou University, Shantou, China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Deng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tian
authors.forename: Xiao-Huan
authors.initials: XH
authors.lastname: Liang
authors.forename: Bao-Chen
authors.initials: BC
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D000818:Animals; D010064:Embryo Implantation / Q000187:drug effects / Q000235:genetics*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005865:Gestational Age; D008297:Male; D051379:Mice; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D014599:Uterus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
chemical_list: C503552:Atp1b1 protein, mouse; D011374:Progesterone; D000254:Sodium-Potassium-Exchanging ATPase