title: Ligand promiscuity within the internal cavity of Epiphyas postvittana Takeout 1 protein.
abstract: Takeout proteins are found across a diverse range of insect species and are thought to be involved in important aspects of insect physiology and behavior. These proteins act as ligand carriers, but the nature of their endogenous ligands remains unknown. The crystal structure of Epiphyas postvittana Takeout 1 (EpTo1), the only structure for any Takeout protein to date, revealed an α/β-wrap fold with a purely hydrophobic internal cavity of tubular shape. When recombinantly expressed in Escherichia coli, we previously showed that a surrogate ubiquinone-8 ligand binds within the internal cavity of EpTo1 with excellent shape complementarity. We have now expressed EpTo1 in an insect cell expression system devoid of ubiquinone-8, and solved its crystal structure at 2.2Å resolution. Using combined information from crystallography and mass spectrometry, we identify a mixture of fatty acid moieties, mostly myristic and palmitic acid, bound inside the EpTo1 cavity, mimicking the structure of the longer ubiquinone-8 compound. No significant alteration of the internal cavity was observed regardless of the bound ligands, ubiquinone-8 or fatty acids, suggesting that the internal cavity of EpTo1 forms a rigid scaffold that imposes strict structural constraints for selectivity and specificity of ligand(s) in vivo.
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand. cyril.hamiaux@plantandfood.co.nz
authors.forename: Cyril
authors.initials: C
authors.lastname: Hamiaux
authors.forename: Lisa
authors.initials: L
authors.lastname: Basten
authors.forename: David R
authors.initials: DR
authors.lastname: Greenwood
authors.forename: Edward N
authors.initials: EN
authors.lastname: Baker
authors.forename: Richard D
authors.initials: RD
authors.lastname: Newcomb
mesh_terms: D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005227:Fatty Acids / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D019476:Insect Proteins / Q000737:chemistry*; D007915:Lepidoptera / Q000737:chemistry; D008024:Ligands; D011487:Protein Conformation; D017510:Protein Folding; D014451:Ubiquinone / Q000737:chemistry / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D005227:Fatty Acids; D019476:Insect Proteins; D008024:Ligands; D014451:Ubiquinone; C030778:ubiquinone 8