title: Central carbon metabolism and electron transport in Chlamydomonas reinhardtii: metabolic constraints for carbon partitioning between oil and starch.
abstract: The metabolism of microalgae is so flexible that it is not an easy task to give a comprehensive description of the interplay between the various metabolic pathways. There are, however, constraints that govern central carbon metabolism in Chlamydomonas reinhardtii that are revealed by the compartmentalization and regulation of the pathways and their relation to key cellular processes such as cell motility, division, carbon uptake and partitioning, external and internal rhythms, and nutrient stress. Both photosynthetic and mitochondrial electron transfer provide energy for metabolic processes and how energy transfer impacts metabolism and vice versa is a means of exploring the regulation and function of these pathways. A key example is the specific chloroplast localization of glycolysis/gluconeogenesis and how it impacts the redox poise and ATP budget of the plastid in the dark. To compare starch and lipids as carbon reserves, their value can be calculated in terms of NAD(P)H and ATP. As microalgae are now considered a potential renewable feedstock, we examine current work on the subject and also explore the possibility of rerouting metabolism toward lipid production.
authors.affiliation: Department of Plant Biology, Carnegie Institution for Science, Stanford, California, USA.
authors.forename: Xenie
authors.initials: X
authors.lastname: Johnson
authors.forename: Jean
authors.initials: J
authors.lastname: Alric
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D004579:Electron Transport; D005943:Gluconeogenesis; D006019:Glycolysis; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010788:Photosynthesis; D010938:Plant Oils / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*
chemical_list: D008055:Lipids; D010938:Plant Oils; D009249:NADP; D002244:Carbon; D000255:Adenosine Triphosphate; D013213:Starch
references.citation: Plant Physiol. 1991 Jun;96(2):355-62
references.pmid: 16668193
references.citation: Arch Biochem Biophys. 1994 Aug 15;313(1):173-8
references.pmid: 8053679
references.citation: Plant Physiol. 2005 Dec;139(4):1995-2005
references.pmid: 16306140
references.citation: FEBS Lett. 1970 Oct 5;10(3):143-148
references.pmid: 11945379
references.citation: Curr Genet. 2011 Jun;57(3):151-68
references.pmid: 21533645
references.citation: Plant Cell Environ. 2010 Nov;33(11):1779-88
references.pmid: 20545877
references.citation: Plant Cell. 1995 Dec;7(12):2139-49
references.pmid: 8718624
references.citation: Eukaryot Cell. 2005 Feb;4(2):242-52
references.pmid: 15701786
references.citation: Plant J. 2008 May;54(4):621-39
references.pmid: 18476868
references.citation: Plant Cell. 2011 Apr;23(4):1273-92
references.pmid: 21498682
references.citation: Mol Cell Proteomics. 2012 Oct;11(10):973-88
references.pmid: 22787274
references.citation: Proc Natl Acad Sci U S A. 2010 May 18;107(20):9458-63
references.pmid: 20439704
references.citation: Curr Opin Biotechnol. 2012 Jun;23(3):352-63
references.pmid: 22209109
references.citation: Eukaryot Cell. 2009 Dec;8(12):1856-68
references.pmid: 19880756
references.citation: Biochim Biophys Acta. 2013 Feb;1827(2):210-23
references.pmid: 22902601
references.citation: Nature. 2009 Nov 26;462(7272):518-21
references.pmid: 19940928
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20546-51
references.pmid: 19074271
references.citation: Biochim Biophys Acta. 1969 Feb 25;172(2):242-51
references.pmid: 5775694
references.citation: Science. 2003 Mar 7;299(5612):1572-5
references.pmid: 12624266
references.citation: J Exp Bot. 2005 Jun;56(416):1469-79
references.pmid: 15863446
references.citation: Plant Cell. 2010 Jan;22(1):221-33
references.pmid: 20081115
references.citation: Biochim Biophys Acta. 2010 Jan;1797(1):44-51
references.pmid: 19651104
references.citation: Eur J Biochem. 1992 Feb 15;204(1):327-36
references.pmid: 1740146
references.citation: Plant J. 2000 Apr;22(1):39-50
references.pmid: 10792819
references.citation: J Bacteriol. 2001 Feb;183(3):1069-77
references.pmid: 11208806
references.citation: Proc Natl Acad Sci U S A. 1988 Mar;85(5):1344-8
references.pmid: 2894027
references.citation: Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20820-5
references.pmid: 22143777
references.citation: FEBS Lett. 1969 Sep;5(1):11-14
references.pmid: 11947226
references.citation: Biochim Biophys Acta. 1974 Oct 18;368(1):97-112
references.pmid: 4423963
references.citation: FEBS Lett. 1995 Nov 6;374(3):351-5
references.pmid: 7589569
references.citation: Plant Physiol. 1985 Sep;79(1):11-7
references.pmid: 16664354
references.citation: Plant Cell. 2011 Jul;23(7):2619-30
references.pmid: 21764992
references.citation: J Exp Bot. 2011 Apr;62(7):2381-92
references.pmid: 21511915
references.citation: Plant Physiol. 1966 May;41(5):885-90
references.pmid: 16656335
references.citation: Mol Cell. 2013 Feb 7;49(3):511-23
references.pmid: 23290914
references.citation: Science. 2007 Oct 12;318(5848):245-50
references.pmid: 17932292
references.citation: Plant Mol Biol. 1984 May;3(3):177-90
references.pmid: 24310351
references.citation: Physiol Plant. 2004 Jan;120(1):21-26
references.pmid: 15032873
references.citation: BMC Biotechnol. 2011 Jan 21;11:7
references.pmid: 21255402
references.citation: BMC Bioinformatics. 2011 Jul 12;12:282
references.pmid: 21749710
references.citation: Mol Biol Cell. 2005 Oct;16(10):4509-18
references.pmid: 16030251
references.citation: Science. 1958 May 2;127(3305):1026-34
references.pmid: 17793272
references.citation: Physiol Plant. 2009 Dec;137(4):371-82
references.pmid: 19558416
references.citation: Arch Biochem Biophys. 1998 Nov 15;359(2):170-8
references.pmid: 9808758
references.citation: Eukaryot Cell. 2011 Dec;10(12):1592-606
references.pmid: 22037181
references.citation: Prog Lipid Res. 2002 Mar;41(2):182-96
references.pmid: 11755683
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10209-14
references.pmid: 12119384
references.citation: J Cell Biol. 2005 Jul 4;170(1):103-13
references.pmid: 15998802
references.citation: Biochemistry. 1995 Feb 28;34(8):2621-7
references.pmid: 7873543
references.citation: Plant Physiol. 2006 Sep;142(1):305-17
references.pmid: 16844835
references.citation: Plant Physiol. 2010 Dec;154(4):1737-52
references.pmid: 20935180
references.citation: J Proteome Res. 2010 Jun 4;9(6):2825-38
references.pmid: 20408572
references.citation: Plant Cell. 1995 Jul;7(7):957-70
references.pmid: 7640528
references.citation: Biochim Biophys Acta. 1975 Aug 11;396(2):260-75
references.pmid: 239745
references.citation: Biochim Biophys Acta. 1969;189(3):366-83
references.pmid: 5370012
references.citation: Biochim Biophys Acta. 1991 Mar 4;1073(2):410-5
references.pmid: 1826218
references.citation: Planta. 2012 Apr;235(4):729-45
references.pmid: 22020754
references.citation: Mol Biol Evol. 2009 Jul;26(7):1533-48
references.pmid: 19349646
references.citation: Metab Eng. 2010 Jul;12(4):387-91
references.pmid: 20172043
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:185-214
references.pmid: 15012287
references.citation: Plant Physiol. 1986 Sep;82(1):160-6
references.pmid: 16664985
references.citation: Planta. 1986 Jan;167(1):81-6
references.pmid: 24241735
references.citation: Plant Physiol. 1983 Jun;72(2):488-91
references.pmid: 16663029
references.citation: Plant Physiol. 1988 Dec;88(4):973-5
references.pmid: 16666488
references.citation: Nature. 2010 Apr 22;464(7292):1210-3
references.pmid: 20364124
references.citation: Plant Physiol. 1989 Jul;90(3):788-91
references.pmid: 16666878
references.citation: Phytochemistry. 2012 Mar;75:50-9
references.pmid: 22226037
references.citation: Plant Physiol. 2000 Apr;122(4):1193-9
references.pmid: 10759515
references.citation: Plant Physiol. 1992 Apr;98(4):1233-8
references.pmid: 16668781
references.citation: Eukaryot Cell. 2010 Jan;9(1):97-106
references.pmid: 19915074
references.citation: Biochim Biophys Acta. 2006 May-Jun;1757(5-6):362-8
references.pmid: 16762315
references.citation: Science. 2005 Jul 22;309(5734):630-3
references.pmid: 16040710
references.citation: Nature. 1965 Jan 2;205:56-7
references.pmid: 14283135
references.citation: Annu Rev Plant Biol. 2011;62:53-77
references.pmid: 21526967
references.citation: Science. 1994 Dec 9;266(5191):1717-9
references.pmid: 7992056
references.citation: Dev Biol. 1973 Mar;31(1):31-7
references.pmid: 4787191
references.citation: J Biol Chem. 2000 Jun 9;275(23):17256-62
references.pmid: 10748104
references.citation: J Lipid Res. 1970 Mar;11(2):131-43
references.pmid: 4392141
references.citation: Curr Genet. 2004 Nov;46(5):304-15
references.pmid: 15459796
references.citation: Proteomics. 2011 Nov;11(21):4266-73
references.pmid: 21928291
references.citation: Proc Natl Acad Sci U S A. 1982 Jul;79(14):4352-6
references.pmid: 16593210
references.citation: Photosynth Res. 2010 Nov;106(1-2):47-56
references.pmid: 20532629
references.citation: Arch Mikrobiol. 1973 Oct 19;93(2):91-100
references.pmid: 4764234
references.citation: Curr Genet. 1991 Feb;19(2):65-71
references.pmid: 2065364
references.citation: Prog Lipid Res. 2010 Apr;49(2):128-58
references.pmid: 19879895
references.citation: Eukaryot Cell. 2010 Aug;9(8):1251-61
references.pmid: 20562225
references.citation: Plant Cell. 2009 Nov;21(11):3473-92
references.pmid: 19897671
references.citation: J Cell Biol. 1980 Apr;85(1):136-45
references.pmid: 6767730
references.citation: J Biotechnol. 2012 Nov 30;162(1):13-20
references.pmid: 22542934
references.citation: J Biotechnol. 2007 Aug 1;131(1):27-33
references.pmid: 17624461
references.citation: Biochim Biophys Acta. 2001 Aug 17;1506(2):133-42
references.pmid: 11522255
references.citation: Annu Rev Plant Biol. 2005;56:73-98
references.pmid: 15862090
references.citation: Plant Cell. 2010 Jun;22(6):2058-84
references.pmid: 20587772
references.citation: FEBS Lett. 2011 Jun 23;585(12):1985-91
references.pmid: 21575636
references.citation: Biochim Biophys Acta. 1989 May 31;991(2):347-52
references.pmid: 2719977
references.citation: J Biol Chem. 2012 Jul 27;287(31):26445-52
references.pmid: 22692199
references.citation: Science. 1968 Nov 15;162(3855):768-71
references.pmid: 4386796
references.citation: Curr Genet. 1990 Aug;18(2):155-60
references.pmid: 2225144
references.citation: J Biol Chem. 2012 May 4;287(19):15811-25
references.pmid: 22403401
references.citation: Nature. 1964 Feb 15;201:725-6
references.pmid: 14134734
references.citation: Biotechnol Bioeng. 2010 Oct 1;107(2):258-68
references.pmid: 20506159