title: Characterization of genes for a putative hydroxycinnamoyl-coenzyme A quinate transferase and p-coumarate 3'-hydroxylase and chlorogenic acid accumulation in tartary buckwheat.
abstract: Tartary buckwheat ( Fagopyrum tataricum Gaertn.) contains a high level of flavonoid compounds, which have beneficial and pharmacological effects on health. In this study, we isolated full-length cDNAs encoding hydroxycinnamoyl-coenzyme A quinate hydroxycinnamoyltransferase (HQT) and p-coumarate 3'-hydroxylase (C3H), which are involved in chlorogenic acid (CGA) biosynthesis. We examined the expression levels of HQT and C3H using real-time RT-PCR in different organs and sprouts of two tartary buckwheat cultivars (Hokkai T8 and T10) and analyzed CGA content using high-performance liquid chromatography. Among the organs, the flowers in both cultivars showed the highest levels of CGA. We concluded that the expression pattern of FtHQT and FtC3H did not match the accumulation pattern of CGA in different organs of T8 and T10 cultivars. Gene expression and CGA content varied between the cultivars. We presume that FtHQT and FtC3H levels might be controlled by multiple metabolic pathways in different organs of tartary buckwheat. Probably, FtC3H might have a greater effect on CGA biosynthesis than FtHQT. Our results will be helpful for a greater understanding of CGA biosynthesis in tartary buckwheat.
authors.affiliation: Department of Crop Science, Chungnam National University , 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Thwe
authors.forename: Ye Ji
authors.initials: YJ
authors.lastname: Kim
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Haeng Hoon
authors.initials: HH
authors.lastname: Kim
authors.forename: Phun Bum
authors.initials: PB
authors.lastname: Park
authors.forename: Tastsuro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics*; D002726:Chlorogenic Acid / Q000737:chemistry*; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics; D019612:Fagopyrum / Q000737:chemistry* / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011801:Quinic Acid / Q000737:chemistry
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D011801:Quinic Acid; D002726:Chlorogenic Acid; D000217:Acyltransferases; C021394:hydroxycinnamoyl-CoA-quinate transferase