title: MiR-29a modulates the angiogenic properties of human endothelial cells.
abstract: Although extensive investigation has been made on miR-29a in relation to malignancies, only a little information has been provided about the angiogenic property of this miRNA so far. Herein, we sought to investigate the role of miR-29a in regulating cell cycle and angiogenic phenotype of endothelial cells. The results showed that miR-29a is highly expressed and upregulated by hypoxia-mimicking reagents in human umbilical vein endothelial cells (HUVEC). Consistent with this preliminary finding, introduction of exogenous agomiR-29a, or Antagomir-29a altered cell cycle progression and promoted, or repressed the proliferation and tube formation of HUVEC, respectively. Furthermore, by using luciferase reporter assay, the expression of HBP1, a suppressor transcription factor was directly regulated by miR-29a through 3'-UTR. Increased or decreased HBP1 protein level was associated with the inhibition or overexpression of miR-29a, respectively. We conclude that miR-29a has a significant role in regulating cell cycle, proliferation and angiogenic properties of HUVEC, and this function is likely mediated through HBP1 protein at the post-transcriptional level. As a novel molecular target, miR-29a may have a potential value for the treatment of angiogenesis-associated diseases such as cardiovascular diseases and cancers.
authors.affiliation: Department of Pathology, Zhejiang University School of Medicine, Hangzhou 310058,China.
authors.forename: Zeran
authors.initials: Z
authors.lastname: Yang
authors.forename: Lingjiao
authors.initials: L
authors.lastname: Wu
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Rong
authors.initials: R
authors.lastname: Jin
authors.forename: Guohong
authors.initials: G
authors.lastname: Li
authors.forename: Fusheng
authors.initials: F
authors.lastname: Wu
mesh_terms: D001483:Base Sequence; D015687:Cell Hypoxia / Q000235:genetics / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism*; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C486328:HBP1 protein, human; D006609:High Mobility Group Proteins; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins
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