title: Influence of ozone on the rheological and electrical properties of stored human blood.
abstract: Blood stored in a blood bank undergoes a series of chemical changes and storage lesions. The purpose of this study was to assess the effect of ozone on the rheological and electrical properties of stored human blood. Venous blood samples, obtained from three healthy humans, were treated with different concentrations of ozone (30, 50, 70 and 80 µg/mL) for three weeks in vitro. Ozone was generated from portable medical-grade oxygen using electrical corona arc discharge. The ultraviolet-visible absorption of hemoglobin in the wavelength of 300-700 nm showed that ozone in this range did not interact with iron ions and it was not toxic below the concentration of 80 µg/mL. The changes of blood viscosity were also measured. The electrical conductivity and permittivity, in the frequency range from 5 to 50 MHz, were measured in the control and treated samples subjected to different concentrations of ozone at different stored periods. The results showed that the conductivity and permittivity measurements may serve as a useful indicator in the quality assessment of blood samples stored in the blood bank.
authors.affiliation: Department of Physics, Faculty of Science, Benha University, Benha 13518, Egypt; ; Department of Physics, Faculty of Science, Taibah University, Al-Ula 100823, Saudia Arabia.
authors.forename: Hoda El-Saied Abdel
authors.initials: Hel-S
authors.lastname: Baieth
authors.forename: Islam Shoukri
authors.initials: IS
authors.lastname: Elashmawi
keywords: alternate current conductivity; blood; ozone generation; viscosity
references.citation: J Med Eng Technol. 1996 May-Jun;20(3):115-20
references.pmid: 8877752
references.citation: Haematologica. 1990 Nov-Dec;75(6):510-5
references.pmid: 2129118
references.citation: Int J Biomed Sci. 2009 Sep;5(3):229-36
references.pmid: 23675142
references.citation: Neuropsychopharmacology. 2001 Sep;25(3):294-8
references.pmid: 11522457
references.citation: Int J Biol Macromol. 2007 Dec 1;41(5):504-11
references.pmid: 17675149
references.citation: FEBS Lett. 2000 Dec 1;486(1):10-3
references.pmid: 11108833
references.citation: J Surg Res. 2002 Jan;102(1):6-12
references.pmid: 11792145
references.citation: Biophys J. 1996 Jul;71(1):495-506
references.pmid: 8804632
references.citation: N Engl J Med. 1971 Feb 18;284(7):353-7
references.pmid: 5539916
references.citation: Free Radic Biol Med. 2005 Mar 1;38(5):547-56
references.pmid: 15683710
references.citation: Int J Biomed Sci. 2008 Dec;4(4):323-9
references.pmid: 23675105
references.citation: Mediators Inflamm. 1994;3(5):315-21
references.pmid: 18475574
references.citation: Vox Sang. 1994;66(4):258-63
references.pmid: 8079449
references.citation: Am J Psychiatry. 2000 Jun;157(6):867-76
references.pmid: 10831463
references.citation: Free Radic Res. 1998 Jun;28(6):553-60
references.pmid: 9736307
references.citation: J Int Med Res. 1994 May-Jun;22(3):131-44
references.pmid: 8088420