title: Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study.
abstract: OBJECTIVE
  To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles.
  
  
  METHODS
  A total of 160 women who underwent vitrified-warmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), or the TE group (AH performing at the site opposite to the ICM). AH with laser zona drilling was performed 20 or 30 min after thawing once the ICM can be detected. Clinical pregnancy rate, implantation rate, live birth rate and the occurrence rate of monozygotic twins (MZT) pregnancy after transfer of these two groups were compared.
  
  
  RESULTS
  No significant difference was found in the clinical pregnancy rate (63.8% vs. 67.5%), implantation rate (51.7% vs. 53.6%) and live birth rate (57.5% vs. 62.5%) between the ICM group and the TE group. The occurrence rate of MZT was comparable between the two groups (3.9% vs. 5.6%).
  
  
  CONCLUSIONS
  The site of assisted hatching has no influence on the implantation, pregnancy and live birth rate in human vitrified-warmed blastocyst transfer cycles.
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Ren
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Guijin
authors.initials: G
authors.lastname: Zhu
authors.forename: Hanwang
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000328:Adult; D001755:Blastocyst; D053624:Blastocyst Inner Cell Mass / Q000166:cytology; D015925:Cryopreservation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008845:Microinjections / Q000009:adverse effects / Q000379:methods*; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000009:adverse effects / Q000379:methods*; D058989:Vitrification; D015044:Zona Pellucida / Q000502:physiology
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