title: Expression profile and mitochondrial colocalization of Tdp1 in peripheral human tissues.
abstract: Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is a DNA repair enzyme that processes blocked 3' ends of DNA breaks. Functional loss of Tdp1 causes spinocerebellar ataxia with axonal neuropathy type 1 (SCAN1). Based on the prominent cytoplasmic expression of Tdp1 in the neurons presumably affected in SCAN1, we hypothesized that Tdp1 participates in the repair of mitochondrial DNA. As a step toward testing this hypothesis, we profiled Tdp1 expression in different human tissues by immunohistochemistry and immunofluorescence respectively and determined whether Tdp1 was expressed in the cytoplasm of tissues other than the neurons. We found that Tdp1 was ubiquitously expressed and present in the cytoplasm of many cell types. Within human skeletal muscle and multiple mouse tissues, Tdp1 partially colocalized with the mitochondria. In cultured human dermal fibroblasts, Tdp1 redistributed to the cytoplasm and partially colocalized with mitochondria following oxidative stress. These studies suggest that one role of cytoplasmic Tdp1 is the repair of mitochondrial DNA lesions arising from oxidative stress.
authors.affiliation: Department of Medical Genetics, Child and Family Research Institute, University of British Columbia, Vancouver, BC, V5Z 4H4, Canada.
authors.forename: Hok Khim
authors.initials: HK
authors.lastname: Fam
authors.forename: Miraj K
authors.initials: MK
authors.lastname: Chowdhury
authors.forename: Cheryl
authors.initials: C
authors.lastname: Walton
authors.forename: Kunho
authors.initials: K
authors.lastname: Choi
authors.forename: Cornelius F
authors.initials: CF
authors.lastname: Boerkoel
authors.forename: Glenda
authors.initials: G
authors.lastname: Hendson
mesh_terms: D000293:Adolescent; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D020405:Dermis / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D009928:Organ Specificity; D018384:Oxidative Stress; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D010727:Phosphoric Diester Hydrolases; C498508:TDP1 protein, human; C524959:Tdp1 protein, mouse
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