title: Synthesis of strong polycations with improved biological properties.
abstract: Poly(allylamine hydrochloride) (PAH) has found many applications both in biotechnology and biomedical fields. However, its high toxicity toward various mammalian cells significantly limits its effective usage. This study focuses on improving the biological properties of PAH by its modification to strong polyelectrolytes. The strong polycations were prepared by the direct quaternization of PAH amino groups or by the attachment of glycidyltrimethylammonium chloride to these groups. The biological properties, such as cytotoxicity toward human skin fibroblasts (HSFs), proliferation and migration of the cells on a polymeric surface, and antibacterial activities against two pathogenic bacteria, Staphylococcus aureus and Escherichia coli, were determined. All the modified polyelectrolytes are considerably less toxic to HSFs as compared to PAH. Moreover, the directly quaternized polycations are stronger biocides against S. aureus than the parent polymer. Contrary to PAH, thin films of the modified polyelectrolytes improve or do not affect HSFs proliferation and can stimulate cell migration into the wound, as was demonstrated using an in vitro model. The relationship between the structure of the modified polymers (amount and localization of the quaternary ammonium groups) and the biological activity is discussed. Due to the improved biological properties, the obtained polycations may be potentially useful for a variety of biotechnological and biomedical applications.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Kraków, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wytrwal
authors.forename: Paulina
authors.initials: P
authors.lastname: Koczurkiewicz
authors.forename: Kinga
authors.initials: K
authors.lastname: Wójcik
authors.forename: Marta
authors.initials: M
authors.lastname: Michalik
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Kozik
authors.forename: Marek
authors.initials: M
authors.lastname: Zylewski
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kepczynski
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000517:prevention & control; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D000071228:Polyelectrolytes; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D011073:Polyamines; D000071228:Polyelectrolytes; C063994:polyallylamine
keywords: antibacterial polymers; cell motility; cytotoxicity; poly(allylamine hydrochloride); polyelectrolytes