title: UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells.
abstract: MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucleotide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB irradiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.
authors.affiliation: Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu 210029, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Wu
authors.initials: W
authors.lastname: Di
authors.forename: Lijuan
authors.initials: L
authors.lastname: Hua
authors.forename: Bingrong
authors.initials: B
authors.lastname: Zhou
authors.forename: Ze
authors.initials: Z
authors.lastname: Guo
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
keywords: HaCaT cells; MicroRNA-141; PTEN; UVB; apoptosis
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