title: Elevated expression of mature miR-21 and miR-155 in cancerous gastric tissues from Chinese patients with gastric cancer.
abstract: OBJECTIVE
  MicroRNA (miRNA) expression is deregulated in many types of human cancers. We sought to investigate the expression patterns of the miRNAs, miR-21, miR-145 and miR-155 in sporadic gastric cancer in a Chinese population.
  
  
  METHODS
  Total RNA was extracted from archived gastric cancer tissues and adjacent non-cancerous tissues from 20 pairs of paraffin-embedded specimens. Expression levels of miR-21, miR-145 and miR-155 were detected by quantitative reverse transcriptase PCR using a specific stem-loop primer, with U6 as the internal reference gene.
  
  
  RESULTS
  The expression of miR-21 and miR-155 in gastric cancer samples was significantly higher than in paired non-cancerous samples (P < 0.05). There was no statistically significant difference in expression levels of miR-145 between cancerous and non-cancerous tissues (P > 0.05).
  
  
  CONCLUSIONS
  In Chinese sporadic gastric cancer tissues, the expressions of the oncogenic miR-21 and miR-155 were significantly up-regulated, while the expression of the tumor suppressor miR-145 was decreased, although this decrease was not statistically significant. Thus there is specificity in the miRNA expression pattern in gastric cancers in the Chinese population.
authors.affiliation: Atherosclerosis Research Center, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China ; Laboratory of Cytology & Molecules/Department of Pathology, the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Rensheng
authors.initials: R
authors.lastname: Lai
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Wu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wu
authors.forename: Fukun
authors.initials: F
authors.lastname: Liu
authors.forename: Guohua
authors.initials: G
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Ji
keywords: MicroRNA; expression; gastric cancer
references.citation: Cancer Res. 2008 Oct 15;68(20):8191-4
references.pmid: 18922889
references.citation: Mol Cancer. 2006 Jul 19;5:29
references.pmid: 16854228
references.citation: Cancer Cell. 2008 Mar;13(3):272-86
references.pmid: 18328430
references.citation: J Cancer Res Clin Oncol. 2009 Aug;135(8):983-9
references.pmid: 19125299
references.citation: Oncogene. 2006 Oct 9;25(46):6188-96
references.pmid: 17028598
references.citation: Ann Surg Oncol. 2009 Nov;16(11):3183-9
references.pmid: 19636633
references.citation: Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15524-9
references.pmid: 12434020
references.citation: Gut. 2009 Oct;58(10):1375-81
references.pmid: 19201770
references.citation: Int J Cancer. 2008 Jul 15;123(2):372-379
references.pmid: 18449891
references.citation: Zhonghua Yi Xue Za Zhi. 2008 Aug 26;88(33):2326-30
references.pmid: 19087692
references.citation: Science. 2005 Nov 25;310(5752):1288-9
references.pmid: 16311325
references.citation: Cancer Res. 2005 Aug 15;65(16):7065-70
references.pmid: 16103053
references.citation: Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16170-5
references.pmid: 17911264
references.citation: Physiol Genomics. 2008 Apr 22;33(2):139-47
references.pmid: 18303086
references.citation: Int J Cancer. 2010 Jan 1;126(1):73-80
references.pmid: 19551852
references.citation: Lab Invest. 2008 Dec;88(12):1358-66
references.pmid: 18794849
references.citation: Thromb Res. 1995 Feb 1;77(3):279-90
references.pmid: 7740520
references.citation: Science. 2007 Apr 27;316(5824):604-8
references.pmid: 17463289
references.citation: Mol Diagn Ther. 2007;11(5):277-90
references.pmid: 17963416
references.citation: J Gastroenterol Hepatol. 2009 Apr;24(4):652-7
references.pmid: 19175831
references.citation: Cancer Res. 2007 Dec 15;67(24):11612-20
references.pmid: 18089790
references.citation: Cancer Cell. 2006 Mar;9(3):189-98
references.pmid: 16530703
references.citation: Int J Clin Pract. 2009 Sep;63(9):1378-85
references.pmid: 19691623
references.citation: Clin Cancer Res. 2008 Apr 15;14(8):2334-40
references.pmid: 18413822
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2257-61
references.pmid: 16461460
references.citation: Gynecol Oncol. 2009 Sep;114(3):457-64
references.pmid: 19501389
references.citation: Trends Genet. 2004 Dec;20(12):617-24
references.pmid: 15522457
references.citation: J Clin Endocrinol Metab. 2008 May;93(5):1600-8
references.pmid: 18270258
references.citation: Cell. 1993 Dec 3;75(5):843-54
references.pmid: 8252621
references.citation: Oncology. 2007;72(5-6):397-402
references.pmid: 18196926
references.citation: Nature. 2004 Nov 11;432(7014):226-30
references.pmid: 15538371
references.citation: Cancer Res. 2006 Aug 1;66(15):7390-4
references.pmid: 16885332
references.citation: Br J Haematol. 2008 May;141(5):672-5
references.pmid: 18318758
references.citation: Zhonghua Wai Ke Za Zhi. 2008 Apr 15;46(8):618-21
references.pmid: 18844061
references.citation: J Gastroenterol. 2009;44 Suppl 19:18-22
references.pmid: 19148788
references.citation: Science. 2004 Jan 2;303(5654):83-6
references.pmid: 14657504
references.citation: Anticancer Res. 2008 Jan-Feb;28(1A):119-23
references.pmid: 18383833
references.citation: J Biol Chem. 2007 Nov 9;282(45):32582-90
references.pmid: 17827156
references.citation: Nucleic Acids Res. 2005 Mar 01;33(4):1290-7
references.pmid: 15741182
references.citation: Int J Biochem Cell Biol. 2010 Aug;42(8):1291-7
references.pmid: 19497386
references.citation: World J Gastroenterol. 2007 May 28;13(20):2883-8
references.pmid: 17569129
references.citation: Clin Chim Acta. 2009 Feb;400(1-2):97-102
references.pmid: 18996365
references.citation: Hepatology. 2008 Apr;47(4):1223-32
references.pmid: 18307259
references.citation: Clin Microbiol Infect. 2009 Sep;15(9):806-12
references.pmid: 19702585
references.citation: Cancer Sci. 2007 Dec;98(12):1914-20
references.pmid: 17892514
references.citation: J Immunother Emphasis Tumor Immunol. 1996 Sep;19(5):357-63
references.pmid: 8941875
references.citation: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references.pmid: 15761078
references.citation: Postepy Biochem. 2007;53(4):413-9
references.pmid: 19024905
references.citation: Transfusion. 2000 Jun;40(6):654-62
references.pmid: 10864984
references.citation: J Virol. 2008 Jun;82(11):5295-306
references.pmid: 18367535
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Dig Dis Sci. 2010 Aug;55(8):2288-93
references.pmid: 19834808
references.citation: Trends Mol Med. 2006 Dec;12(12):580-7
references.pmid: 17071139
references.citation: Forensic Sci Int. 2000 Sep 11;113(1-3):47-53
references.pmid: 10978601
references.citation: Endocr Pathol. 2007 Fall;18(3):163-73
references.pmid: 18058265
references.citation: Methods. 2008 Jan;44(1):39-46
references.pmid: 18158131
references.citation: Cancer Genet Cytogenet. 2008 Feb;181(1):8-15
references.pmid: 18262046