title: Bisdemethoxycurcumin inhibits PDGF-induced vascular smooth muscle cell motility and proliferation.
abstract: METHODS
  A key event in the development of plaque in the arteries is the migration and proliferation of smooth muscle cells (SMCs) from the media to the intima of the blood vessel. This study was conducted to evaluate the effects of bisdemethoxycurcumin (BC), a naturally occurring structural analog of curcumin (CC), on platelet-derived growth factor (PDGF)-stimulated migration and proliferation of SMCs.
  
  
  RESULTS
  CC and BC were synthesized by condensing acetyl acetone with vanillin and 4-hydroxybenzaldehyde, respectively. SMCs isolated from adult rat aorta were stimulated with PDGF in the presence or absence of CC or BC following which, cell migration and proliferation were assessed by monolayer wound healing assay and [(3) H]-thymidine incorporation respectively. PDGF-stimulated phosphorylation of PDGF receptor-β and its downstream effectors Akt and ERK were assessed by Western blotting. Intracellular reactive oxygen species was assessed using the fluorescent dye 5-(6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate. BC elicited a concentration-dependent inhibition of PDGF-stimulated phosphorylation of PDGF receptor-β, Akt and Erk as well as the PDGF-stimulated SMC migration and proliferation. BC was more potent than CC in inhibiting migration and proliferation and suppressing PDGF-signaling in SMCs. Both compounds were equipotent in inhibiting PDGF-stimulated generation of intracellular reactive oxygen species.
  
  
  CONCLUSIONS
  BC may be of potential use in the prevention or treatment of vascular disease.
authors.affiliation: School of Pharmacy, College of Health Sciences, Division of Pharmaceutical Sciences & The Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY, USA.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Hua
authors.forename: Julia
authors.initials: J
authors.lastname: Dolence
authors.forename: Shalini
authors.initials: S
authors.lastname: Ramanan
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Sreejayan
authors.initials: S
authors.lastname: Nair
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000494:pharmacology; D036381:Diarylheptanoids; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001547:Benzaldehydes; D036381:Diarylheptanoids; D010982:Platelet-Derived Growth Factor; D017382:Reactive Oxygen Species; C034786:bisdemethoxycurcumin; C100058:vanillin; D020797:Receptor, Platelet-Derived Growth Factor beta; D051057:Proto-Oncogene Proteins c-akt; D003474:Curcumin; C011483:4-hydroxybenzaldehyde
keywords: Curcumin; Migration; Platelet-derived growth factor; Proliferation; Vascular smooth muscle cell
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