title: Separation of actin-dependent and actin-independent lipid rafts.
abstract: Lipid rafts have been isolated on the basis of their resistance to various detergents and more recently by using detergent-free procedures. The actin cytoskeleton is now recognized as a dynamic regulator of lipid raft stability. We carefully analyzed the effects of the cortical actin-disrupting agent latrunculin B on lipid raft markers of both protein and lipid nature and show that two detergent-free membrane subtypes can be isolated and separated from each other on a one-step density gradient combined with pooling of the appropriate gradient fractions. These two subtypes differ in their dependence on the cortical actin cytoskeleton.
authors.affiliation: Department of Cell Biology, University Medical Center Groningen, University of Groningen, 9713 AV Groningen, The Netherlands.
authors.forename: Karin
authors.initials: K
authors.lastname: Klappe
authors.forename: Ina
authors.initials: I
authors.lastname: Hummel
authors.forename: Jan Willem
authors.initials: JW
authors.lastname: Kok
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000378:metabolism; D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry*; D051379:Mice; D013107:Sphingolipids / Q000737:chemistry / Q000378:metabolism; D053778:Thiazolidines
chemical_list: D018528:ATP-Binding Cassette Transporters; D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D013107:Sphingolipids; D053778:Thiazolidines; D002784:Cholesterol; C037068:latrunculin B