title: In vivo and in vitro antioxidant activity and α-glucosidase, α-amylase inhibitory effects of flavonoids from Cichorium glandulosum seeds.
abstract: The aim of this study was to investigate the antioxidant, anti-glucosidase and anti-amylase activities of total flavonoids (TFs) from Cichorium glandulosum seeds, and to analyse its chemical composition by HPLC-ESI/MS. In vitro study, radical scavenging IC50 values of TFs were 7.33±0.093, 9.24±0.100, 154.33±11.38 and 256.7±4.86 μg/ml for DPPH, ABTS, hydroxyl radicals, and superoxide anion, respectively. In the 8-64 mg/ml range, α-glucosidase and α-amylase were inhibited by TFs to a certain extent. In vivo, the treatment groups with TFs (100, 200, 400 mg/kg) showed a significant decrease in the malondialdehyde level, the superoxide dismutase and glutathione levels were restored to almost normal levels, and the catalase and glutathione peroxidase levels significantly increased compared to the CCl4-intoxicated group in rats. The present study suggests that C. glandulosum seeds should be given special attention because of their antioxidant and anti-glucosidase, anti-amylase activity.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of TCM & Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Yao
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
authors.forename: Youwei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D019659:Asteraceae / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000008:administration & dosage* / Q000737:chemistry; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008107:Liver Diseases / Q000188:drug therapy* / Q000201:enzymology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D005419:Flavonoids; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases