title: microRNAs in uterine sarcomas and mixed epithelial-mesenchymal uterine tumors: a preliminary report.
abstract: Uterine sarcomas and mixed epithelial-mesenchymal uterine tumors are a heterogeneous group of rare tumors for which there are very few diagnostic markers available. As aberrant microRNA (miRNA) expression patterns represent putative diagnostic cancer markers, we aimed to identify miRNA expression profiles of the major uterine sarcoma subtypes and mixed epithelial-mesenchymal tumors of the uterus. Eighty-eight miRNAs were assessed by quantitative RT-PCR in cancerous and non-cancerous tissue samples collected from 29 patients with endometrial sarcoma, leiomyosarcoma, and mixed epithelial-mesenchymal tumors. Tumor and control samples significantly (P < 0.05) differed in the expression of miR-23b, miR-1, let-7f, and let-7c in endometrial sarcomas, and miR-1, let-7c, miR-133b, let-7b, miR-143, let-7a, let-7d, let-7e, let-7g, miR-222, let-7i, and miR-214 in mixed epithelial-mesenchymal tumors. All the significantly changed miRNAs were down-regulated in the malignant tissues as compared to their normal counterparts. This may suggest their tumor suppressor role in these malignancies. No statistically significant changes in miRNA expression levels were found between leiomyosarcoma tumors and controls. The identified miRNAs warrant further studies as valuable candidate markers for the differential diagnosis of uterine sarcomas from benign uterine lesions and between uterine sarcoma subtypes.
authors.affiliation: Department of Molecular Biology, Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology, Roentgena 5, 02-781, Warsaw, Poland. magdak@coi.waw.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kowalewska
authors.forename: Elwira
authors.initials: E
authors.lastname: Bakula-Zalewska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chechlinska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Goryca
authors.forename: Anna
authors.initials: A
authors.lastname: Nasierowska-Guttmejer
authors.forename: Anna
authors.initials: A
authors.lastname: Danska-Bidzinska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Bidzinski
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000175:diagnosis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007890:Leiomyosarcoma / Q000175:diagnosis / Q000235:genetics; D008637:Mesenchymoma / Q000175:diagnosis / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis / Q000235:genetics; D012509:Sarcoma / Q000175:diagnosis / Q000235:genetics; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
references.citation: PLoS One. 2011;6(8):e22839
references.pmid: 21829658
references.citation: Gynecol Oncol. 2002 Apr;85(1):165-9
references.pmid: 11925138
references.citation: Genes Chromosomes Cancer. 2007 Apr;46(4):336-47
references.pmid: 17243163
references.citation: Oncogene. 2010 Sep 2;29(35):4914-24
references.pmid: 20562915
references.citation: J Clin Invest. 2009 Aug;119(8):2366-78
references.pmid: 19620785
references.citation: Nature. 2010 Aug 12;466(7308):835-40
references.pmid: 20703300
references.citation: J Cell Mol Med. 2008 Jan-Feb;12(1):227-40
references.pmid: 18182067
references.citation: J Biol Chem. 2012 May 4;287(19):15672-83
references.pmid: 22431733
references.citation: J Hepatol. 2010 Jul;53(1):98-107
references.pmid: 20447717
references.citation: Blood. 2010 Nov 18;116(20):4338-48
references.pmid: 20709907
references.citation: Cancer Res. 2007 Jul 1;67(13):6031-43
references.pmid: 17616659
references.citation: Curr Genomics. 2009 Mar;10(1):35-41
references.pmid: 19721809
references.citation: Cancer Res. 2011 Oct 1;71(19):6230-9
references.pmid: 21816906
references.citation: Endocr Relat Cancer. 2010 Jan 29;17(1):F19-36
references.pmid: 19779035
references.citation: Gynecol Oncol. 2010 Jan;116(1):131-9
references.pmid: 19853898
references.citation: J Exp Med. 2010 Jul 5;207(7):1351-8
references.pmid: 20513743
references.citation: Mod Pathol. 2011 Oct;24(10):1368-79
references.pmid: 21572397
references.citation: Int J Gynecol Cancer. 2011 Jan;21(1):8-14
references.pmid: 21330826
references.citation: Cancer Lett. 2009 Jan 18;273(2):233-42
references.pmid: 18789835
references.citation: Am J Obstet Gynecol. 2001 May;184(6):1051-3
references.pmid: 11349153
references.citation: Lancet Oncol. 2009 Dec;10(12):1188-98
references.pmid: 19959075
references.citation: Oncol Rep. 2010 Jul;24(1):195-201
references.pmid: 20514462
references.citation: J Mol Cell Cardiol. 2010 Nov;49(5):841-50
references.pmid: 20708014
references.citation: Mol Hum Reprod. 2007 Nov;13(11):797-806
references.pmid: 17766684
references.citation: Science. 2007 Dec 21;318(5858):1931-4
references.pmid: 18048652
references.citation: Cancer Cell. 2008 Nov 4;14(5):369-81
references.pmid: 18977326
references.citation: Cancer. 1993 Feb 15;71(4 Suppl):1702-9
references.pmid: 8381710
references.citation: Oncogene. 2008 Mar 27;27(14):2015-26
references.pmid: 17922033
references.citation: Oncogene. 2010 Apr 8;29(14):2153-9
references.pmid: 20101213
references.citation: Gynecol Oncol. 2010 Sep;118(3):251-7
references.pmid: 20542546
references.citation: Gynecol Oncol. 2004 Apr;93(1):204-8
references.pmid: 15047237
references.citation: Nat Commun. 2011 Nov 22;2:554
references.pmid: 22109528