title: Comparison of bone regeneration using three demineralized freeze-dried bone allografts: A histological and histomorphometric study in rabbit calvaria.
abstract: BACKGROUND
  It has been stated that the bone allografts from different tissue banks may lead to various amount of bone induction, so the aim of this study was to evaluate bone regeneration of three demineralized allografts both histologically and histomorphometrically in rabbits calvaria bone defects.
  
  
  METHODS
  In this double-blind randomized experimental animal study, 32 critical size defects (11-mm diameter) in the calvaria of 16 male New Zealand white rabbits were randomly filled with three demineralized freeze-dried bone allografts (DBM, CENOBONE, DEMBONE), while the nongrafted defect was regarded as control group. After 6 and 12 weeks of healing, the experimental animals were euthanized for specimen preparation. After histological evaluation, histomorphometric analysis was performed to quantify new bone formation and remained graft particles. The data were analyzed by one-way ANOVA with Tukey's ad-hoc test and t-test. (P < 0.05 was considered to be statistically significant).
  
  
  RESULTS
  Mean percentage of bone formation increased between two healing time, but it was not statistically significant in all groups except DBM which the bone formation significantly decreased (P = 0.04). There were not statistically significant differences between three allografts in remained particles and bone formation in both healing times and they could not induce significantly more bone formation than control group.
  
  
  CONCLUSIONS
  Both test and control groups resulted in successful new bone formation. No difference was noted in bone formation and remained particles between three commercial bone allografts. Further studies in this issue may be needed.
authors.affiliation: Torabinejad Dental Research Center and Department of Periodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Parichehr
authors.initials: P
authors.lastname: Behfarnia
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shahabooei
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mashhadiabbas
authors.forename: Elham
authors.initials: E
authors.lastname: Fakhari
keywords: Bone regeneration; demineralized freeze-dried bone allografts; osteoinduction; tissue bank
references.citation: Cell Tissue Bank. 2011 May;12(2):81-8
references.pmid: 20039143
references.citation: J Periodontal Implant Sci. 2010 Jun;40(3):132-8
references.pmid: 20607058
references.citation: Clin Oral Investig. 2011 Apr;15(2):225-32
references.pmid: 20054593
references.citation: Plast Reconstr Surg. 2000 Apr;105(4):1382-92
references.pmid: 10744229
references.citation: J Periodontol. 1998 Apr;69(4):470-8
references.pmid: 9609378
references.citation: Spine (Phila Pa 1976). 1989 Dec;14(12):1324-31
references.pmid: 2617362
references.citation: Arch Orthop Trauma Surg. 1978 Feb 10;91(1):3-9
references.pmid: 148877
references.citation: Open Dent J. 2011;5:96-104
references.pmid: 21760862
references.citation: Cell Tissue Bank. 2011 Aug;12(3):171-84
references.pmid: 20473718
references.citation: Ann Surg. 1889 Nov;10(5):352-68
references.pmid: 17857417
references.citation: J Dent Res. 2011 Sep;90(9):1052-61
references.pmid: 21248364
references.citation: J Craniofac Surg. 1990 Jan;1(1):60-8
references.pmid: 1965154
references.citation: J Dent Res Dent Clin Dent Prospects. 2008 Winter;2(1):1-8
references.pmid: 23285322
references.citation: Aust Dent J. 2011 Jun;56 Suppl 1:107-18
references.pmid: 21564121
references.citation: Orthopedics. 2004 May;27(5):488-95; quiz 496-7
references.pmid: 15181946
references.citation: Injury. 2007 Sep;38 Suppl 4:S13-21
references.pmid: 18224733
references.citation: J Periodontal Implant Sci. 2010 Aug;40(4):180-7
references.pmid: 20827327
references.citation: Int J Periodontics Restorative Dent. 1996 Dec;16(6):524-37
references.pmid: 9242091
references.citation: J Periodontol. 1989 Jan;60(1):12-8
references.pmid: 2537897
references.citation: J Oral Implantol. 2006;32(2):55-62
references.pmid: 16704106
references.citation: Proc Natl Acad Sci U S A. 1983 Nov;80(21):6591-5
references.pmid: 6579546
references.citation: Arch Orthop Trauma Surg. 2001 Nov;121(10):583-90
references.pmid: 11768641
references.citation: J Periodontol. 2002 Feb;73(2):213-9
references.pmid: 11895288
references.citation: Clin Orthop Relat Res. 1967 Jan-Feb;50:291-308
references.pmid: 4291315
references.citation: J Periodontol. 1994 Dec;65(12):1128-33
references.pmid: 7877084
references.citation: J Oral Maxillofac Surg. 2004 Feb;62(2):202-13
references.pmid: 14762753
references.citation: Arch Orthop Trauma Surg. 1982;100(2):73-8
references.pmid: 6814400
references.citation: J Periodontol. 1996 Sep;67(9):918-26
references.pmid: 8884650