title: Deconstruction of medulloblastoma cellular heterogeneity reveals differences between the most highly invasive and self-renewing phenotypes.
abstract: Medulloblastoma (MB) is the most common malignant primary pediatric brain tumor. Major research efforts have focused on characterizing and targeting putative brain tumor stem or propagating cell populations from the tumor mass. However, less is known about the relationship between these cells and highly invasive MB cells that evade current therapies. Here, we dissected MB cellular heterogeneity and directly compared invasion and self-renewal. Analysis of higher versus lower self-renewing tumor spheres and stationary versus migrating adherent MB cells revealed differential expression of the cell surface markers CD271 [p75 neurotrophin receptor (p75NTR)] and CD133. Cell sorting demonstrated that CD271 selects for subpopulations with a higher capacity for self-renewal, whereas CD133 selects for cells exhibiting increased invasion in vitro. CD271 expression is higher in human fetal cerebellum and primary samples of the Shh MB molecular variant and lower in the more aggressive, invasive group 3 and 4 subgroups. Global gene expression analysis of higher versus lower self-renewing MB tumor spheres revealed down-regulation of a cell movement transcription program in the higher self-renewing state and a novel potential role for axon guidance signaling in MB-propagating cells. We have identified a cell surface signature based on CD133/CD271 expression that selects for MB cells with a higher self-renewal potential or invasive capacity in vitro. Our study underscores a previously unappreciated role for CD271 in selecting for MB cell phenotypes and suggests that successful treatment of pediatric brain tumors requires concomitant targeting of a spectrum of transitioning self-renewing and highly infiltrative cell subpopulations.
authors.affiliation: Regenerative Medicine Program, Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Ludivine Coudière
authors.initials: LC
authors.lastname: Morrison
authors.forename: Robyn
authors.initials: R
authors.lastname: McClelland
authors.forename: Christopher
authors.initials: C
authors.lastname: Aiken
authors.forename: Melissa
authors.initials: M
authors.lastname: Bridges
authors.forename: Lisa
authors.initials: L
authors.lastname: Liang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Domenico
authors.initials: D
authors.lastname: Di Curzio
authors.forename: Marc R
authors.initials: MR
authors.lastname: Del Bigio
authors.forename: Michael D
authors.initials: MD
authors.lastname: Taylor
authors.forename: Tamra E
authors.initials: TE
authors.lastname: Werbowetski-Ogilvie
mesh_terms: D000328:Adult; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D051927:CD24 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D002528:Cerebellar Neoplasms / Q000378:metabolism / Q000473:pathology*; D019008:Drug Resistance, Neoplasm; D059472:Exome; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D053650:Interleukin-3 Receptor alpha Subunit / Q000378:metabolism; D008527:Medulloblastoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D018874:Spheroids, Cellular / Q000378:metabolism / Q000502:physiology
chemical_list: D000964:Antimetabolites, Antineoplastic; D051927:CD24 Antigen; C115022:IL3RA protein, human; D053650:Interleukin-3 Receptor alpha Subunit; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor; D005472:Fluorouracil
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