title: Striatal glutamate induces retrograde excitotoxicity and neuronal degeneration of intralaminar thalamic nuclei: their potential relevance for Parkinson's disease.
abstract: An over-stimulation of nigral glutamate (GLU) receptors has been proposed as a cause of the progression of the dopamine (DA) cell degeneration (excitotoxicity) which characterizes Parkinson's disease. The possible toxic action of striatal GLU (retrograde excitotoxicity) on these cells, and on other neurons which innervate the striatum and which also degenerate in Parkinson's disease (thalamostriatal cells of the intralaminar thalamic nuclei), is still practically unexplored. The retrograde excitotoxicity of striatal GLU on DAergic mesostriatal and GLUergic thalamostriatal cells was tested here by studying these cells 6 weeks after striatal perfusion of GLU by reverse microdialysis. GLU perfusion induced the striatal denervation of thalamic inputs (as revealed by vesicular glutamate transporter 2) and the remote degeneration of intralaminar neurons. In both centres, these effects were accompanied by microglial activation. Similar responses were not observed for nigrostriatal neurons, which showed no dopaminergic striatal denervation, no microglial activation in the substantia nigra and no changes in the number of dopaminergic cells in the substantia nigra. The inhibition of DAergic transmission increased the extrasynaptic GLU levels in the striatum (evaluated by microdialysis), an effect observed after the local administration of agonists and antagonists of DAergic transmission, and after the peripheral administration of levodopa (which increased the DA and decreased the GLU levels in the striatum of rats with an experimental DAergic denervation of this centre). The data presented show that striatal GLU induced a retrograde excitotoxicity which did not affect all striatal inputs in the same way and which could be involved in the cell degeneration of the intralaminar nuclei of the thalamus generally observed in Parkinson's disease.
authors.affiliation: Laboratory of Neurobiology and Experimental Neurology, Department of Physiology, Faculty of Medicine, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Morales
authors.forename: Magdalena
authors.initials: M
authors.lastname: Sabate
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodriguez
mesh_terms: D000200:Action Potentials; D000818:Animals; D016923:Cell Death; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D015259:Dopamine Agents / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism*; D020646:Intralaminar Thalamic Nuclei / Q000166:cytology / Q000378:metabolism*; D007980:Levodopa / Q000494:pharmacology; D008297:Male; D017628:Microglia / Q000378:metabolism / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000378:metabolism / Q000502:physiology; D009435:Synaptic Transmission; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism
chemical_list: D015259:Dopamine Agents; D050599:Vesicular Glutamate Transport Protein 2; D018698:Glutamic Acid; D007980:Levodopa; D004298:Dopamine
keywords: Parkinson's disease; glutamate; intralaminar thalamic nuclei; retrograde excitotoxicity; striatum; substantia nigra