title: Plastic protein microarray to investigate the molecular pathways of magnetic nanoparticle-induced nanotoxicity.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) (about 15 nm) were synthesized via a hydrothermal method and characterized by field emission scanning electron microscopy, transmission electron microscopy, dynamic light scattering, x-ray diffraction, and vibrating sample magnetometer. The molecular pathways of SPIONs-induced nanotoxicity was further investigated by protein microarrays on a plastic substrate from evaluation of cell viability, reactive oxygen species (ROS) generation and cell apoptosis. The experimental results reveal that 50 μg ml(-1) or higher levels of SPIONs cause significant loss of cell viability, considerable generation of ROS and cell apoptosis. It is proposed that high level SPIONs could induce cell apoptosis via a mitochondria-mediated intrinsic pathway by activation of caspase 9 and caspase 3, an increase of the Bax/Bcl-2 ratio, and down-regulation of HSP70 and HSP90 survivor factors.
authors.affiliation: Institute for Clean Energy and Advanced Materials, Southwest University, Chongqing, People's Republic of China.
authors.forename: Yingshuai
authors.initials: Y
authors.lastname: Liu
authors.forename: Xuelian
authors.initials: X
authors.lastname: Li
authors.forename: Shujuan
authors.initials: S
authors.lastname: Bao
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D016716:PC12 Cells; D040081:Protein Array Analysis / Q000379:methods; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D058185:Magnetite Nanoparticles; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D053148:Caspase 3; D053453:Caspase 9