title: Isolation of Arcobacter species in water buffaloes (Bubalus bubalis).
abstract: This is the first report of Arcobacter spp. in rectal fecal samples from healthy water buffaloes (Bubalus bubalis) reared on a dairy farm. Arcobacter species were isolated after enrichment, and isolates were identified at species level by multiplex-polymerase chain reaction assay. Thirty samples were examined and Arcobacter spp. were isolated from 96.7% of water buffaloes tested: 38 Arcobacter spp. isolates were obtained, with A. cryaerophilus as the dominant species followed by A. butzleri and A. skirrowii. Nine animals (31%) were colonized by more than one Arcobacter species. The present study indicates that water buffaloes can harbor a variety of Arcobacter spp. and that healthy buffaloes may act as hosts. Water buffalo fecal shedding of Arcobacter spp. may be of significance to human health, considering the potential fecal contamination during harvesting of raw milk and slaughtering.
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, Bologna, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Piva
authors.forename: Andrea
authors.initials: A
authors.lastname: Serraino
authors.forename: Daniela
authors.initials: D
authors.lastname: Florio
authors.forename: Federica
authors.initials: F
authors.lastname: Giacometti
authors.forename: Frederique
authors.initials: F
authors.lastname: Pasquali
authors.forename: Gerardo
authors.initials: G
authors.lastname: Manfreda
authors.forename: Renato Giulio
authors.initials: RG
authors.lastname: Zanoni
mesh_terms: D000818:Animals; D020640:Arcobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D057138:Bacterial Shedding; D002020:Buffaloes / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D003612:Dairying; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D020699:Gastroscopes; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008460:Meat / Q000382:microbiology*; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial