title: CRL2(LRR-1) E3-ligase regulates proliferation and progression through meiosis in the Caenorhabditis elegans germline.
abstract: The ubiquitin-proteolytic system controls the stability of proteins in space and time. In this study, using a temperature-sensitive mutant allele of the cul-2 gene, we show that CRL2(LRR-1) (CUL-2 RING E3 ubiquitin-ligase and the Leucine Rich Repeat 1 substrate recognition subunit) acts at multiple levels to control germline development. CRL2(LRR-1) promotes germ cell proliferation by counteracting the DNA replication ATL-1 checkpoint pathway. CRL2(LRR-1) also participates in the mitotic proliferation/meiotic entry decision, presumably controlling the stability of meiotic promoting factors in the mitotic zone of the germline. Finally, CRL2(LRR-1) inhibits the first steps of meiotic prophase by targeting in mitotic germ cells degradation of the HORMA domain-containing protein HTP-3, required for loading synaptonemal complex components onto meiotic chromosomes. Given its widespread evolutionary conservation, CUL-2 may similarly regulate germline development in other organisms as well.
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Burger
authors.forename: Jorge
authors.initials: J
authors.lastname: Merlet
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tavernier
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Richaudeau
authors.forename: Andreas
authors.initials: A
authors.lastname: Arnold
authors.forename: Rafal
authors.initials: R
authors.lastname: Ciosk
authors.forename: Bruce
authors.initials: B
authors.lastname: Bowerman
authors.forename: Lionel
authors.initials: L
authors.lastname: Pintard
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000378:metabolism; D049109:Cell Proliferation; D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D008540:Meiosis / Q000235:genetics*; D008938:Mitosis; D010770:Phosphotransferases / Q000378:metabolism; D013573:Synaptonemal Complex / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D044842:Cullin Proteins; C527587:HTP-3 protein, C elegans; C491027:cul-2 proteins, C elegans; C576885:Atl-1 protein, C elegans; D010770:Phosphotransferases; D064007:Ataxia Telangiectasia Mutated Proteins
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