title: Evaluation of tropicamide-loaded tamarind seed xyloglucan nanoaggregates for ophthalmic delivery.
abstract: The present study was aimed to prepare tamarind seed nanoaggregates and its evaluation for ophthalmic delivery. The preparation of tropicamide-loaded tamarind seed xyloglucan nanoaggregates was optimized using face centred central composite experimental design, employing the concentrations of tamarind seed xyloglucan and Poloxamer-407, as independent variables. The results revealed that concentration of TSX has a significant antagonistic effect on particle size, while poloxamer displayed a significant synergistic effect on encapsulation efficiency. The optimal concentrations of TSX and poloxamer were found to be 0.45% (w/v) and 0.5% (w/v) respectively. The optimized formulation of tropicamide-loaded TSX nanoaggregates showed a significantly higher corneal permeation of tropicamide across the isolated goat cornea compared to commercial conventional aqueous formulation. The results revealed excellent mucoadhesive properties of TSX nanoaggregates. Further, the tropicamide-loaded TSX nanoaggregates formulation showed excellent ocular tolerance and biocompatibility as determined by hen's egg test chorioallantoic membrane and resazurin assay on Vero cell lines.
authors.affiliation: Nanomaterials Synthesis & Characterization Laboratory, Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar 125001, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Dilbaghi
authors.forename: Harmanmeet
authors.initials: H
authors.lastname: Kaur
authors.forename: Munish
authors.initials: M
authors.lastname: Ahuja
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
mesh_terms: D060433:Administration, Ophthalmic; D000327:Adsorption; D000818:Animals; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D049033:Chorioallantoic Membrane / Q000378:metabolism; D003315:Cornea / Q000378:metabolism; D004339:Drug Compounding; D005936:Glucans / Q000737:chemistry* / Q000633:toxicity; D006041:Goats; D008422:Materials Testing; D009184:Mydriatics / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D053769:Nanocapsules / Q000737:chemistry* / Q000633:toxicity; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D029861:Tamarindus / Q000737:chemistry*; D014331:Tropicamide / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D014709:Vero Cells; D014990:Xylans / Q000737:chemistry* / Q000633:toxicity
chemical_list: D005936:Glucans; D009184:Mydriatics; D053769:Nanocapsules; D010936:Plant Extracts; D014990:Xylans; C029353:xyloglucan; D014331:Tropicamide