title: Comparative study of 1,064-nm laser-induced skin burn and thermal skin burn.
abstract: Infrared lasers are widely used in medicine, industry, and other fields. While science, medicine, and the society in general have benefited from the many practical uses of lasers, they also have inherent safety issues. Although several procedures have been put forward to protect the skin from non-specific laser-induced damage, individuals receiving laser therapy or researchers who use laser are still at risk for skin damage. This study aims to understand the interaction between laser and the skin, and to investigate the differences between the skin damage caused by 1,064-nm laser and common thermal burns. Skin lesions on Wistar rats were induced by a 1,064-nm CW laser at a maximum output of 40 W and by a copper brass bar attached to an HQ soldering iron. Histological sections of the lesions and the process of wound healing were evaluated. The widths of the epidermal necrosis and dermal denaturalization of each lesion were measured. To observe wound healing, the epithelial gap and wound gap were measured. Masson's trichrome and picrosirius red staining were also used to assess lesions and wound healing. The thermal damage induced by laser intensified significantly in both horizontal dimension and in vertical depth with increased duration of irradiation. Ten days after wounding, the dermal injuries induced by laser were more severe. Compared with the laser-induced skin damage, the skin burn induced by an HQ soldering iron did not show a similar development or increased in severity with the passage of time. The results of this study showed the pattern of skin damage induced by laser irradiation and a heated brass bar. This study also highlighted the difference between laser irradiation and thermal burn in terms of skin damage and wound healing, and offers insight for further treatment.
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Ruan
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Huang
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D006358:Hot Temperature; D007834:Lasers; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology / Q000528:radiation effects*; D014945:Wound Healing
chemical_list: D003094:Collagen