title: Effects of matrine on oval cell‑mediated liver regeneration and expression of RBP‑Jκ and HES1.
abstract: In the present study a rat model of oval cell‑mediated liver regeneration was constructed to examine the molecular mechanisms of matrine in oval cell‑mediated liver regeneration and the effects of matrine on hepatic function and the expression of OV6 protein and recombination signal sequence‑binding protein Jκ (RBP‑Jκ) and HES1 mRNA. A total of 48 Sprague Dawley rats were equally and randomly assigned to two groups. The model group underwent oval cell‑mediated liver regeneration, whereas the matrine group underwent oval cell‑mediated liver regeneration and received oral gavage of matrine. Expression of OV6 protein was tested by immunohistochemistry and RBP‑Jκ and HES1 mRNA expression was determined by reverse‑transcription polymerase chain reaction. Recovery of hepatic function was faster in the matrine group compared with the model (P<0.05). OV6 protein, RBP‑Jκ and HES1 mRNA expression levels were lower in the matrine than the model group (P<0.05). Matrine promotes oval cell‑mediated liver regeneration through downregulation of the RBP‑Jκ‑HES1 signaling pathway.
authors.affiliation: Department of Traditional Chinese Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, PR China. zhiyunyang@yeah.net
authors.forename: Zhi-Yun
authors.initials: ZY
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Hou
authors.forename: Xian-Bo
authors.initials: XB
authors.lastname: Wang
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002452:Cell Count; D005786:Gene Expression Regulation / Q000187:drug effects; D006498:Hepatectomy; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000601:surgery; D008111:Liver Function Tests; D008115:Liver Regeneration / Q000187:drug effects* / Q000235:genetics; D008297:Male; D011807:Quinolizines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016019:Survival Analysis; D000072056:Transcription Factor HES-1; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000943:Antigens, Differentiation; D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D011807:Quinolizines; D012333:RNA, Messenger; D000072056:Transcription Factor HES-1; D000093842:Matrines; C489735:Hes1 protein, rat; C115011:oval cell marker OV-6