title: The involvement of Helicobacter pylori thioredoxin-1 in gastric carcinogenesis.
abstract: Helicobacter pylori infection is related to the development of gastric diseases. Various virulence factors are responsible for the pathogenic mechanisms of H. pylori infection. Our previous studies using two-dimensional gel electrophoresis showed that H. pylori thioredoxin-1 (Trx1) is overexpressed in gastric carcinomas. Here, we examined whether H. pylori Trx1 is a novel virulence factor associated with gastric tumorigenesis. We found that Trx1 expression in H. pylori isolated from gastric cancer tissues was significantly higher than that from tissues exhibiting gastritis. In the gastric epithelial cell line GES-1, infection of H. pylori with high Trx1 expression significantly induced cell apoptosis, decreased the expression of cyclin D1 and upregulated p21. However, in the gastric cancer cell line BGC823, high Trx1 expression in H. pylori significantly increased cell proliferation, and upregulated cyclin D1. The effects on cell lines were confirmed using the H. pylori Trx1-knockout mutant strain. Our observations indicate that high Trx1 expression in H. pylori is associated with gastric carcinogenesis. In H. pylori, Trx1 likely participates in the pathogenesis of gastric cancer and H. pylori expressing high levels of Trx1 would be expected to be highly pathogenic in gastric diseases in China.
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Linna
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Microbiology, Peking University Health Science Center, Beijing, 100191, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Qinghai University, Xining, 810001, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Microbiology, Peking University Health Science Center, Beijing, 100191, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Yuexia
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Qinghai University, Xining, 810001, China.
authors.forename: Xuehong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Qinghai University, Xining, 810001, China.
authors.forename: Shengsen
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Microbiology, Peking University Health Science Center, Beijing, 100191, China.
authors.forename: Fengmin
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Microbiology, Peking University Health Science Center, Beijing, 100191, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Shigang
authors.initials: S
authors.lastname: Ding
mesh_terms: D017209:Apoptosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D002681:China; D019938:Cyclin D1 / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D005756:Gastritis / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D016481:Helicobacter Infections / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D009154:Mutation; D009928:Organ Specificity; D013274:Stomach Neoplasms / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C530104:CCND1 protein, human; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D037521:Virulence Factors; D019938:Cyclin D1; D013879:Thioredoxins