title: Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.
abstract: Detailed characterization of the gene expression patterns in spermatogonia and primary spermatocytes is critical to understand the processes which occur prior to meiosis during normal spermatogenesis. The genome-wide expression profiles of mouse type B spermatogonia and primary spermatocytes were investigated using the Solexa/Illumina digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental processes which occur during early spermatogenesis were systematically analyzed. Gene expression patterns vary significantly between mouse type B spermatogonia and primary spermatocytes. The functional analysis revealed that genes related to junction assembly, regulation of the actin cytoskeleton and pluripotency were most significantly differently expressed. Pathway analysis indicated that the Wnt non-canonical signaling pathway played a central role and interacted with the actin filament organization pathway during the development of spermatogonia. This study provides a foundation for further analysis of the gene expression patterns and signaling pathways which regulate the molecular mechanisms of early spermatogenesis.
authors.affiliation: College of Life Sciences, Hebei United University, Tangshan, Hebei, China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Hao
authors.forename: Lijun
authors.initials: L
authors.lastname: Meng
authors.forename: Meiling
authors.initials: M
authors.lastname: Liu
authors.forename: Lina
authors.initials: L
authors.lastname: Zhao
authors.forename: Fen
authors.initials: F
authors.lastname: Hu
authors.forename: Cunbao
authors.initials: C
authors.lastname: Ding
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Baoling
authors.initials: B
authors.lastname: He
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
authors.forename: Mengchun
authors.initials: M
authors.lastname: Jia
mesh_terms: D000818:Animals; D002460:Cell Line; D019295:Computational Biology; D030561:Databases, Nucleic Acid; D051523:Fibroblast Growth Factor 7 / Q000235:genetics / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D022001:Focal Adhesions / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases; D015203:Reproducibility of Results; D015398:Signal Transduction; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics* / Q000502:physiology; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D008565:Membrane Proteins; D020928:Mitogen-Activated Protein Kinases; D051153:Wnt Proteins; D020744:rac GTP-Binding Proteins; C491628:Sfrp2 protein, mouse; D000094742:Secreted Frizzled-Related Proteins; C108111:fibroblast growth factor 13
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