title: Expression, purification, crystallization and preliminary X-ray analysis of tannase from Lactobacillus plantarum.
abstract: Tannase catalyses the hydrolysis of the galloyl ester bond of tannins to release gallic acid. It belongs to the serine esterases and has wide applications in the food, feed, beverage, pharmaceutical and chemical industries. The tannase from Lactobacillus plantarum was cloned, expressed and purified. The protein was crystallized by the sitting-drop vapour-diffusion method with microseeding. The crystals belonged to space group P1, with unit-cell parameters a = 46.5, b = 62.8, c = 83.8 Å, α = 70.4, β = 86.0, γ = 79.4°. Although the enzyme exists mainly as a monomer in solution, it forms a dimer in the asymmetric unit of the crystal. The crystals diffracted to beyond 1.60 Å resolution using synchrotron radiation and a complete data set was collected to 1.65 Å resolution.
authors.affiliation: Institute of Nano-biomedical Technology and Membrane Biology, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Mingbo
authors.initials: M
authors.lastname: Wu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Peng
authors.forename: Hua
authors.initials: H
authors.lastname: Wen
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Qianming
authors.initials: Q
authors.lastname: Chen
authors.forename: William J
authors.initials: WJ
authors.lastname: McKinstry
authors.forename: Bin
authors.initials: B
authors.lastname: Ren
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D048191:Lactiplantibacillus plantarum / Q000201:enzymology*
chemical_list: D002265:Carboxylic Ester Hydrolases; C022711:tannase
keywords: Lactobacillus plantarum; hydrolase; tannase; tannin
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