title: Using surface-assisted laser desorption/ionization mass spectrometry to detect ss- and ds-oligodeoxynucleotides.
abstract: We applied surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) with HgTe nanostructures as the matrix for the detection of single- and double-stranded oligodeoxynucleotides (ss-ODNs and ds-ODNs). The concentrations of surfactant and additives (metal ions, an amine) and the pH and ionic strength of the sample matrix played significantly different roles in the detection of ss- and ds-ODNs with various sequences. In the presence of Brij 76 (1.5 %), Hg(2+) (7.5 μM), and cadaverine (10 μM) at pH 5.0, this SALDI-MS approach allowed the simultaneous detection of T15, T20, T33, and T40, with limits of detection at the femtomole-to-picomole level and sample-to-sample intensity variation <23 %. In the presence of Ag(+) (1 μM) and cadaverine (10 μM) at pH 7.0, this technique allowed the detection of randomly sequenced ss- and ds-ODNs at concentrations down to the femtomole level. To the best of our knowledge, this paper is the first to report the detection of ss-ODNs (up to 50-mer) and ds-ODNs (up to 30 base pairs) through the combination of SALDI-MS with HgTe nanostructures as matrices. We demonstrated the practicality of this approach through analysis of a single nucleotide polymorphism that determines the fate of the valine residue in the β-globin of sickle cell megaloblasts.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Wen-Tsen
authors.initials: WT
authors.lastname: Chen
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Huang
authors.forename: Huan-Tsung
authors.initials: HT
authors.lastname: Chang
mesh_terms: D002103:Cadaverine / Q000737:chemistry; D019343:Citric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D019216:Metals, Heavy / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D019216:Metals, Heavy; D009838:Oligodeoxyribonucleotides; D000644:Quaternary Ammonium Compounds; D019343:Citric Acid; C481046:ammonium citrate; D002103:Cadaverine
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