title: In vitro leishmanicidal activity of some Cameroonian medicinal plants.
abstract: Eleven plants used in the Cameroonian traditional medicine for the treatment of some parasitic infections were tested for their activity on the promastigote form of Leishmania donovani. After incubation with different plant extracts at doses of 1600, 800, 400 and 200 microgram/mL, the evaluation of the cell viability was done by the trypan blue exclusion technique and by flow cytometry. This study shows that 48 h after incubation of promastigotes with plant extract, Solanocia mannii and Solanum torvum significantly inhibited the proliferation of promastigotes in culture with IC50 of 60.78±5.05 and 96.08±4.39 using the trypan blue exclusion technique. In addition, IC50 of 43.91±6.49 and 86.13±4.30 were obtained using the flow cytometry technique. Furthermore, 24 h after incubation of promastigotes with the Solanocia mannii and Solanum torvum, there was significant disruption of their long spindle shaped bodies. The results of this study support the popular uses of these plants for the treatment of some parasitic infections in Cameroonian folk medicine.
authors.affiliation: Department of Biomedical Sciences, Faculty of Science P.O. Box 067, University of Dschang, Cameroon. donfackhubert@yahoo.fr
authors.forename: Donfack J
authors.initials: DJ
authors.lastname: Hubert
authors.forename: Nkenfou
authors.initials: N
authors.lastname: Céline
authors.forename: Noubom
authors.initials: N
authors.lastname: Michel
authors.forename: V Reddy
authors.initials: VR
authors.lastname: Gogulamudi
authors.forename: Ngueguim T
authors.initials: NT
authors.lastname: Florence
authors.forename: Boampong N
authors.initials: BN
authors.lastname: Johnson
authors.forename: Ngadjui T
authors.initials: NT
authors.lastname: Bonaventure
authors.forename: Inder P
authors.initials: IP
authors.lastname: Singh
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sehgal
mesh_terms: D002163:Cameroon; D005434:Flow Cytometry; D020128:Inhibitory Concentration 50; D007893:Leishmania donovani / Q000187:drug effects*; D007898:Leishmaniasis, Visceral / Q000188:drug therapy; D016488:Medicine, African Traditional / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry*; D032322:Solanum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts