title: A sequence in the Drosophila H3-H4 Promoter triggers histone locus body assembly and biosynthesis of replication-coupled histone mRNAs.
abstract: Compartmentalization of RNA biosynthetic factors into nuclear bodies (NBs) is a ubiquitous feature of eukaryotic cells. How NBs initially assemble and ultimately affect gene expression remains unresolved. The histone locus body (HLB) contains factors necessary for replication-coupled histone messenger RNA transcription and processing and associates with histone gene clusters. Using a transgenic assay for ectopic Drosophila HLB assembly, we show that a sequence located between, and transcription from, the divergently transcribed H3-H4 genes nucleates HLB formation and activates other histone genes in the histone gene cluster. In the absence of transcription from the H3-H4 promoter, "proto-HLBs" (containing only a subset of HLB components) form, and the adjacent histone H2a-H2b genes are not expressed. Proto-HLBs also transiently form in mutant embryos with the histone locus deleted. We conclude that HLB assembly occurs through a stepwise process involving stochastic interactions of individual components that localize to a specific sequence in the H3-H4 promoter.
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Harmony R
authors.initials: HR
authors.lastname: Salzler
authors.forename: Deirdre C
authors.initials: DC
authors.lastname: Tatomer
authors.forename: Pamela Y
authors.initials: PY
authors.lastname: Malek
authors.forename: Stephen L
authors.initials: SL
authors.lastname: McDaniel
authors.forename: Anna N
authors.initials: AN
authors.lastname: Orlando
authors.forename: William F
authors.initials: WF
authors.lastname: Marzluff
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Duronio
mesh_terms: D000818:Animals; D004261:DNA Replication; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D006657:Histones; D012333:RNA, Messenger
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