title: Real-time evaluation of binding mechanisms in multivalent interactions: a surface plasmon resonance kinetic approach.
abstract: Multivalency is a key, ubiquitous phenomenon in nature characterized by a complex combination of binding mechanisms, with special relevance in carbohydrate-lectin recognition. Herein we introduce an original surface plasmon resonance kinetic approach to analyze multivalent interactions that has been validated with dendrimers as monodisperse multivalent analytes binding to lectin clusters. The method, based on the analysis of early association and late dissociation phases of the sensorgrams provides robust information of the glycoconjugate binding efficiency and real-time structural data of the binding events under the complex scenario of the glyco-cluster effect. Notably, it reveals the dynamic nature of the interaction and offers experimental evidence on the contribution of binding mechanisms.
authors.affiliation: Department of Organic Chemistry and Center for Research in Biological Chemistry and Molecular Materials, University of Santiago de Compostela, Jenaro de la Fuente s/n, 15782 Santiago de Compostela, Spain. evamaria.munoz@usc.es
authors.forename: Eva Maria
authors.initials: EM
authors.lastname: Munoz
authors.forename: Juan
authors.initials: J
authors.lastname: Correa
authors.forename: Ricardo
authors.initials: R
authors.lastname: Riguera
authors.forename: Eduardo
authors.initials: E
authors.lastname: Fernandez-Megia
mesh_terms: D000465:Algorithms; D003208:Concanavalin A / Q000737:chemistry; D050091:Dendrimers; D005707:Gallic Acid / Q000737:chemistry; D007700:Kinetics; D016014:Linear Models; D008956:Models, Chemical; D011092:Polyethylene Glycols / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D050091:Dendrimers; D003208:Concanavalin A; C028914:triethylene glycol; D011092:Polyethylene Glycols; D005707:Gallic Acid