title: Development of a chemical probe for identifying protein targets of α-oxoaldehydes.
abstract: The development of a chemical probe for identifying the protein targets of reactive electrophilic α-oxoaldehydes such as methylglyoxal is presented. The probe is evaluated against methylglyoxal using human serum albumin as well as using living cells and lysates.
authors.affiliation: Department of Chemistry, Aarhus University, Langelandsgade 140, 8000 Aarhus C, Denmark.
authors.forename: Christian
authors.initials: C
authors.lastname: Sibbersen
authors.forename: Johan
authors.initials: J
authors.lastname: Palmfeldt
authors.forename: Jakob
authors.initials: J
authors.lastname: Hansen
authors.forename: Niels
authors.initials: N
authors.lastname: Gregersen
authors.forename: Karl Anker
authors.initials: KA
authors.lastname: Jørgensen
authors.forename: Mogens
authors.initials: M
authors.lastname: Johannsen
mesh_terms: D000480:Alkynes / Q000032:analysis / Q000737:chemistry*; D000595:Amino Acid Sequence; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D011765:Pyruvaldehyde / Q000032:analysis*; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D000480:Alkynes; D005456:Fluorescent Dyes; D012709:Serum Albumin; D011765:Pyruvaldehyde