title: Improvement of the lipophilic-oxygen radical absorbance capacity (L-ORAC) method and single-laboratory validation.
abstract: We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used. The antioxidant capacities of food extracts can be evaluated by this method with nearly the same precision as antioxidant solutions.
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan. nabej@affrc.go.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Oki
authors.forename: Jun
authors.initials: J
authors.lastname: Takebayashi
authors.forename: Koji
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takano-Ishikawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hino
authors.forename: Akemi
authors.initials: A
authors.lastname: Yasui
mesh_terms: D016166:Free Radical Scavengers / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007753:Laboratories; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals