title: Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity.
abstract: In the present study, we achieved silver nanoparticles using the aqueous extract of Origanum vulgare (Oregano) by reducing 1mM silver nitrate (AgNO3) solution. The green synthesized silver nanoparticles were characterized by high throughput techniques like UV-vis spectroscopy, Fourier infrared spectroscopy (FT-IR), field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD) and dynamic light scattering measurements. Morphologically, the nanoparticles were found to be spherical with an average particle size distribution of 136±10.09nm. FT-IR spectral analysis illustrates the occurrence of possible biomolecules required for the reduction of silver ions. The obtained nanoparticles were stable (-26±0.77mV) at ambient temperature. The biosynthesized nanoparticles were found to be impressive in inhibiting human pathogens. The green synthesized silver nanoparticles showed dose dependent response against human lung cancer A549 cell line (LD50 - 100μg/ml).
authors.affiliation: Department of Biochemistry, School of Life Science, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Sankar
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Karthik
authors.forename: Annamalai
authors.initials: A
authors.lastname: Prabu
authors.forename: Selvaraju
authors.initials: S
authors.lastname: Karthik
authors.forename: Kanchi Subramanian
authors.initials: KS
authors.lastname: Shivashangari
authors.forename: Vilwanathan
authors.initials: V
authors.lastname: Ravikumar
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D031341:Origanum / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts; D012834:Silver