title: Quantitative proteomics of auditory fear conditioning.
abstract: Auditory fear conditioning is a well-characterized rodent learning model where a neutral auditory cue is paired with an aversive outcome to induce associative fear memory. The storage of long-term auditory fear memory requires long-term potentiation (LTP) in the lateral amygdala and de novo protein synthesis. Although many studies focused on individual proteins have shown their contribution to LTP and fear conditioning, non-biased genome-wide studies have only recently been possible with microarrays, which nevertheless fall short of measuring changes at the level of proteins. Here we employed quantitative proteomics to examine the expression of hundreds of proteins in the lateral amygdala in response to auditory fear conditioning. We found that various proteins previously implicated in LTP, learning and axon/dendrite growth were regulated by fear conditioning. A substantial number of proteins that were regulated by fear conditioning have not yet been studied specifically in learning or synaptic plasticity.
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Ingie
authors.initials: I
authors.lastname: Hong
authors.forename: Taewook
authors.initials: T
authors.lastname: Kang
authors.forename: Ki Na
authors.initials: KN
authors.lastname: Yun
authors.forename: YongCheol
authors.initials: Y
authors.lastname: Yoo
authors.forename: Sungmo
authors.initials: S
authors.lastname: Park
authors.forename: Jihye
authors.initials: J
authors.lastname: Kim
authors.forename: Bobae
authors.initials: B
authors.lastname: An
authors.forename: Sukwoon
authors.initials: S
authors.lastname: Song
authors.forename: Sukwon
authors.initials: S
authors.lastname: Lee
authors.forename: Jeongyeon
authors.initials: J
authors.lastname: Kim
authors.forename: Beomjong
authors.initials: B
authors.lastname: Song
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Kwon
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Young Mok
authors.initials: YM
authors.lastname: Park
authors.forename: Sukwoo
authors.initials: S
authors.lastname: Choi
mesh_terms: D000161:Acoustic Stimulation; D000679:Amygdala / Q000502:physiology; D000818:Animals; D003213:Conditioning, Psychological / Q000502:physiology*; D005239:Fear / Q000502:physiology*; D008297:Male; D057567:Memory, Long-Term / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000502:physiology; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D009419:Nerve Tissue Proteins