title: Simultaneous speciation and preconcentration of ultra trace concentrations of mercury and selenium species in environmental and biological samples by hollow fiber liquid phase microextraction prior to high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry.
abstract: Nowadays, hollow fiber membrane extraction techniques are widely used due to the high enrichment factors obtained with many different types of analytes and samples. In this paper, we propose a new analytical method that allows the simultaneous extraction of methylmercury, inorganic mercury and Se(4+) and determination by high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The detection limits obtained are very low (110-230ng/L) with relative standard deviations below 15% for all the analytes and averaged recoveries in fortified samples in the range of 71-99%. The precision of the analytical method is very good which overcomes one of the most important shortcomings of membrane extraction techniques. Several variables were studied to get optimal extraction conditions for the analytes. This method has been validated with real world samples such as water (tap, river and estuarine) and human blood plasma.
authors.affiliation: Department of Chemistry and Materials Science, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Moreno
authors.forename: T
authors.initials: T
authors.lastname: García-Barrera
authors.forename: J L
authors.initials: JL
authors.lastname: Gómez-Ariza
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D060766:Drinking Water / Q000737:chemistry; D006801:Humans; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D008628:Mercury / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D008767:Methylmercury Compounds / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry; D012643:Selenium / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D060766:Drinking Water; D008767:Methylmercury Compounds; D012997:Solvents; D014874:Water Pollutants, Chemical; D012965:Sodium Chloride; D008628:Mercury; D012643:Selenium
keywords: Hollow-fiber; Human plasma; ICP-MS; Membrane extraction; Mercury; Selenium; Water