title: A DNA microarray for the detection of point mutations and copy number variation causing familial hypercholesterolemia in Europe.
abstract: To facilitate genetic cascade screening for familial hypercholesterolemia (FH) in Europe, two versions (7 and 9) of a DNA microarray were developed to detect the most frequent point mutations in the low-density lipoprotein receptor (LDLR), apolipoprotein B (APOB), and proprotein convertase subtilisin/kexin 9 (PCSK9) genes. The design of these microarrays is based on LIPOchip, version 4, which detects 191 LDLR and APOB mutations identified in Spanish patients with FH. A major improvement of LIPOchip, versions 7 and 9, is the ability to detect copy number variation (deletions or duplications of entire exons) in LDLR, thus abolishing the need to perform multiplex ligase-dependent probe amplification in patients with FH. The aim of this study was to validate a tool capable of detecting point mutations and copy number variations simultaneously and to evaluate its use and the newly developed software for analysis in clinical practice by reanalysis of several patients with known mutations causing FH. With the help of these validations, several aspects were analyzed, improved, and implemented in a newer version, which was evaluated through an internal validation.
authors.affiliation: Progenika Biopharma SA, Derio, Spain. mstef@progenika.com
authors.forename: Marianne A
authors.initials: MA
authors.lastname: Stef
authors.forename: Lourdes
authors.initials: L
authors.lastname: Palacios
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Olano-Martín
authors.forename: Carolyn
authors.initials: C
authors.lastname: Foe-A-Man
authors.forename: Laura
authors.initials: L
authors.lastname: van de Kerkhof
authors.forename: Lisette N
authors.initials: LN
authors.lastname: Klaaijsen
authors.forename: Araitz
authors.initials: A
authors.lastname: Molano
authors.forename: Ellen J
authors.initials: EJ
authors.lastname: Schuurman
authors.forename: Diego
authors.initials: D
authors.lastname: Tejedor
authors.forename: Joep C
authors.initials: JC
authors.lastname: Defesche
mesh_terms: D001055:Apolipoproteins B / Q000235:genetics; D056915:DNA Copy Number Variations; D005060:Europe / Q000453:epidemiology; D005091:Exons; D005260:Female; D005820:Genetic Testing; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017354:Point Mutation; D000071449:Proprotein Convertase 9; D043484:Proprotein Convertases / Q000235:genetics; D011973:Receptors, LDL / Q000235:genetics*; D015203:Reproducibility of Results; D012697:Serine Endopeptidases / Q000235:genetics; D013030:Spain / Q000453:epidemiology
chemical_list: D001055:Apolipoproteins B; C573412:LDLR protein, human; D011973:Receptors, LDL; C472125:PCSK9 protein, human; D000071449:Proprotein Convertase 9; D043484:Proprotein Convertases; D012697:Serine Endopeptidases