title: Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.
abstract: B cells were first discovered as antibody producing cells, as B-1 B cells and finally as effector cells. In recent years their capacity to serve as antigen presenting cells is increasingly appreciated, and better tools are needed to study their function. We have previously described a new mouse model, the iDTR mice, that allow for the Cre-mediated expression of the diphtheria toxin receptor, thus rendering cells that express the Cre-recombinase sensitivity to diphtheria toxin. Herein we describe a new mouse line, the B-DTR mice, where the CD19-Cre was crossed to the iDTR mice. B-DTR allows for the efficient and cost-effective depletion of different B cell subpopulations, but only partially plasma cells. These mice can therefore be used to study the importance of B cells versus plasma cells in different immune responses and autoimmune diseases.
authors.affiliation: Institute for Molecular Medicine, University Medical Center of the Johannes-Gutenberg, University of Mainz, Mainz, Germany.
authors.forename: Filiz
authors.initials: F
authors.lastname: Demircik
authors.forename: Thorsten
authors.initials: T
authors.lastname: Buch
authors.forename: Ari
authors.initials: A
authors.lastname: Waisman
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D004167:Diphtheria Toxin / Q000494:pharmacology*; D066257:Heparin-binding EGF-like Growth Factor; D007136:Immunoglobulins / Q000097:blood; D019426:Integrases / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism; D018655:Lymphocyte Count; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010950:Plasma Cells / Q000187:drug effects / Q000378:metabolism; D053668:Syndecan-1 / Q000378:metabolism
chemical_list: D018941:Antigens, CD19; D001426:Bacterial Proteins; D004167:Diphtheria Toxin; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D007136:Immunoglobulins; D036341:Intercellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; D053668:Syndecan-1; C054562:yellow fluorescent protein, Bacteria; C045073:Cre recombinase; D019426:Integrases
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