title: Immunocapture couples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid detection of type 1 dengue virus.
abstract: A facile method for accurate detection of type 1 dengue virus (DV1) infection from complex biological mixtures, using type specific immunocapture coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed. The biological mixtures were treated with magnetic particles coated with a monoclonal antibody directly against type 1 dengue virus. After immunocapture purification, the DV1 was eluted with 30% acetic acid, directly spotted with seed-layer method, and analyzed by MALDI-TOF MS for DV1 capsid protein. The detection limit of the assay was ∼10(5)pfu/mL by MALDI-TOF MS. The immunocapture could unambiguously differentiate the DV1 from other serotypes of the dengue viruses and Japanese encephalitis virus, and could be used as a specific probe to detect DV1 from complex biological mixtures.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan.
authors.forename: Wen-Hung
authors.initials: WH
authors.lastname: Chen
authors.forename: I-Hsiang
authors.initials: IH
authors.lastname: Hsu
authors.forename: Yuh-Chang
authors.initials: YC
authors.lastname: Sun
authors.forename: Yu-Kuo
authors.initials: YK
authors.lastname: Wang
authors.forename: Tung-Kung
authors.initials: TK
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D003716:Dengue Virus / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014759:Viral Envelope Proteins / Q000097:blood / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins