title: Construction, expression, and characterization of thymosin alpha 1 tandem repeats in Escherichia coli.
abstract: Thymosin alpha 1 (T α 1), which is composed of 28 amino acids, has been commercialized worldwide for its immune-modulatory and antitumor effects. T α 1 can stimulate T cell proliferation and differentiation from bone marrow stem cells, augment cell-mediated immune responses, and regulate homeostasis of immune system. In this study, we developed a novel strategy to produce T α 1 concatemer (T α 1③) in Escherichia coli and compared its activity with chemically synthesized T α 1. Results showed that T α 1③ can more effectively stimulate T cell proliferation and significantly upregulate IL-2 receptor expression. We concluded that the expression system for T α 1 concatemer was constructed successfully, which could serve as an efficient tool for the production of large quantities of the active protein.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. xue_xiaochang@yahoo.com
authors.forename: Xiao-Chang
authors.initials: XC
authors.lastname: Xue
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Qin
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hao
authors.forename: Zeng-Lu
authors.initials: ZL
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ying-Qi
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism; D020080:Tandem Repeat Sequences / Q000235:genetics*; D013879:Thioredoxins / Q000096:biosynthesis / Q000235:genetics; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D013879:Thioredoxins; D013947:Thymosin; D017346:Protein Serine-Threonine Kinases; C496588:STK36 protein, human; D000077596:Thymalfasin
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