title: The reliability of clinical dynamic monitoring of redox status using a new redox potential (ORP) determination method.
abstract: OBJECTIVE
  Quantitative monitoring of the redox status is the foundation for redox-related treatment. The purpose of this study was to evaluate the reliability of a new depolarization curve method for plasma redox potential (ORP) monitoring.
  
  
  METHODS
  Using the new method, we performed redox determinations for the first time under different sample-handling conditions, including redox titration experiments using KMnO4 and vitamin C and dynamic redox monitoring in burn patients. The relative ORP value (ΔORP) method (improved traditional method) was used as the reference.
  
  
  RESULTS
  The new method's better reliability, electrochemical specificity and practicability, and known group validity, which are closely associated with the redox-related pathological processes of severe burns, were confirmed. Furthermore, bidirectional change in the redox status in severe burn patients was also observed for the first time.
  
  
  CONCLUSIONS
  This simple, stable new method could be a better practical tool for making the dynamic monitoring of the redox status feasible and for providing useful quantitative information for the judgment of redox-related pathological process, thus improving corresponding individualized treatments that rely on quantitative adjustments to the redox status.
authors.affiliation: Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China. zlizhu@yahoo.com
authors.forename: Lizhu
authors.initials: L
authors.lastname: Zhi
authors.forename: Juan
authors.initials: J
authors.lastname: Liang
authors.forename: Xinlei
authors.initials: X
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Chaoheng
authors.initials: C
authors.lastname: Yu
authors.forename: Huawei
authors.initials: H
authors.lastname: Shao
authors.forename: Xuanliang
authors.initials: X
authors.lastname: Pan
authors.forename: Chunmao
authors.initials: C
authors.lastname: Han
mesh_terms: D015998:Abbreviated Injury Scale; D000328:Adult; D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D002056:Burns / Q000097:blood* / Q000150:complications / Q000378:metabolism; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D005260:Female; D006801:Humans; D008297:Male; D008706:Methemoglobin / Q000032:analysis; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011196:Potassium Permanganate / Q000032:analysis / Q000737:chemistry; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000209:etiology / Q000378:metabolism; D012769:Shock / Q000097:blood / Q000209:etiology / Q000378:metabolism; D014527:Uric Acid / Q000097:blood
chemical_list: D011196:Potassium Permanganate; D014527:Uric Acid; D008706:Methemoglobin; D001205:Ascorbic Acid
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