title: Clinical study of tumor angiogenesis and perfusion imaging using multi-slice spiral computed tomography for breast cancer.
abstract: OBJECTIVE
  To explore the correlation between multi-slice spiral CT (MSCT) perfusion parameters and the expression of vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase-2 (MMP- 2) in breast cancer.
  
  
  METHODS
  Forty five breast cancer patients and 16 patients with benign breast tumor, both confirmed by pathology examination, were enrolled. All underwent MSCT perfusion imaging to obtain perfusion maps and data for parameters including blood flow (BF), blood volume (BV) and permeability surface (PS). Cancer patients did not receive treatment prior to surgery. The expression of VEGF and MMP-2 were examined with both immunohistochemistry and Western blotting.
  
  
  RESULTS
  The levels of VEGF and MMP-2 by immunohistochemistry were significantly higher in the breast cancer group (P < 0.01) than the benign tumor group. Relative OD values from Western blotting were also higher in cancer cases (P < 0.05). Similarly, the mean MSCT perfusion parameters (BF, BV, PS) were significantly higher in the breast cancer group (P < 0.01), BF and BV positively correlating with VEGF expression (r = 0.878 and 0.809 respectively, P < 0.01); PS and VEGF and MMP-2 expression were also positively correlated (r= 0.860, 0.786 respectively, P < 0.01).
  
  
  CONCLUSIONS
  There is a correlation between breast cancer MSCT perfusion parameters and VEGF andMMP-2 expression, which might be useful for detection of breast lesions, qualitative diagnosis of breast cancer, and evaluation of breast cancer treatment.
authors.affiliation: Department of Radiology, The First Hospital of Liaoning Medical College, Jinzhou, China.
authors.forename: Na
authors.initials: N
authors.lastname: Xu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lei
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Guo-Quan
authors.initials: GQ
authors.lastname: Feng
authors.forename: Di-Nuo
authors.initials: DN
authors.lastname: Li
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Bian
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Zou
mesh_terms: D000328:Adult; D000368:Aged; D001810:Blood Volume; D001940:Breast / Q000098:blood supply* / Q000000981:diagnostic imaging; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000503:physiopathology*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008327:Mammography; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000378:metabolism; D055420:Perfusion Imaging; D012039:Regional Blood Flow; D036542:Tomography, Spiral Computed; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2