title: The expression of BRCA1, P53, KAI1, and Nm23 in ovaries of BRCA1 mutation carriers after prophylactic adnexectomy.
abstract: BACKGROUND
  High mortality rate, absence of reliable methods for early diagnosis and poor prognosis of advanced ovarian cancer prompted to investigate the role of prophylactic oophorectomy in BRCA1 mutation carriers as well as evaluate the expression of BRCA1, p53, Nm23, and KAI1 proteins in ovarian tissue from these patients.
  
  
  METHODS
  Ovaries from BRCA1 mutation carriers underwent prophylactic adnexectomy and control group of patients were operated from other than cancer reasons. The expression of selected proteins was studied using immunohistochemical staining. The intensity of immunostaining and the number of tumor cells showing the reaction for selected proteins were analyzed.
  
  
  RESULTS
  We have analyzed ovarian tissues from 18 BRCA1 mutation carriers and 11 women included in control group. Positive expression of BRCA1 protein was presented in 83.3 % cases in BRCA1 mutation carriers and in 72.7 % in the control group (p > 0.05). Positive expression of p53 protein was observed, respectively, in 27.8 vs. 36.4 % (p > 0.05), Nm23 protein 77.7 vs. 90.9 % (p > 0.05), and KAI1 in 72.2 vs. 72.7 % (p > 0.05). Mean percent of tumor cells that showed the reaction for selected proteins as well as the intensity of immunostaining for all analyzed proteins seems to be lower in BRCA1 mutation carriers.
  
  
  CONCLUSIONS
  However, any significant differences between study group and control group have not been found; there were similar trends showing reduced expression of studied proteins in BRCA1 mutation carriers.
authors.affiliation: Division of Gynecology, Department of Oncology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Markowska
authors.forename: J
authors.initials: J
authors.lastname: Bar
authors.forename: R
authors.initials: R
authors.lastname: Mądry
authors.forename: I
authors.initials: I
authors.lastname: Słomska
authors.forename: M
authors.initials: M
authors.lastname: Mardas
authors.forename: J P
authors.initials: JP
authors.lastname: Grabowski
mesh_terms: D000328:Adult; D019313:BRCA1 Protein / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D019398:Genes, BRCA1; D020022:Genetic Predisposition to Disease; D061325:Hereditary Breast and Ovarian Cancer Syndrome / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D006801:Humans; D007150:Immunohistochemistry; D051861:Kangai-1 Protein / Q000378:metabolism; D008875:Middle Aged; D009154:Mutation; D054778:NM23 Nucleoside Diphosphate Kinases / Q000378:metabolism; D010052:Ovariectomy; D010053:Ovary / Q000378:metabolism* / Q000601:surgery; D058994:Salpingectomy; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D014408:Biomarkers, Tumor; C497688:CD82 protein, human; D051861:Kangai-1 Protein; D054778:NM23 Nucleoside Diphosphate Kinases; D016159:Tumor Suppressor Protein p53; C517938:NME1 protein, human
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