title: The surface microporosity of ceramic biomaterials influences the resorption capacity of osteoclasts.
abstract: This study investigated the influence of the surface microporosity of beta-tri-calcium phosphate (β-TCP) ceramics on the resorption capacity of osteoclasts. This was achieved by first compacting commercially available β-TCP powder into disks that were sintered at various temperatures, thereby yielding different surface microporosities. Scanning electron microscopy (SEM) and subsequent image processing verified different degrees of surface microporosity on the disks. Rabbit osteoclasts in a bone marrow derived cell suspension were then seeded onto these disks and incubated for 48 h. Tartrate resistant acid phosphatase (TRAP) staining confirmed the presence of osteoclasts on all disks. Actin ring staining that detected actively resorbing OCs showed an inverse linear correlation between the number of actively resorbing osteoclasts (percentage of total OCs on the surfaces) with surface microporosity. These findings should be taken into consideration for the design and/or production of new β-TCP bone graft substitutes.
authors.affiliation: Baxter Innovations GmbH, Industriestrasse 67, 1220 Vienna, Austria.
authors.forename: Gerald
authors.initials: G
authors.lastname: Zimmer
authors.forename: Astrid
authors.initials: A
authors.lastname: Rohrhofer
authors.forename: Kanthi
authors.initials: K
authors.lastname: Lewis
authors.forename: Andreas
authors.initials: A
authors.lastname: Goessl
authors.forename: Oskar
authors.initials: O
authors.lastname: Hoffmann
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001862:Bone Resorption / Q000473:pathology*; D002130:Calcium Phosphates / Q000494:pharmacology; D002452:Cell Count; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D010010:Osteoclasts / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D016062:Porosity; D011817:Rabbits; D013194:Staining and Labeling; D000071681:Tartrate-Resistant Acid Phosphatase; D014961:X-Ray Diffraction
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; C018392:tricalcium phosphate
keywords: bone graft; calcium phosphate; ceramic structure; osteoclast; surface microporosity