title: Crystallization and preliminary X-ray crystallographic studies of the CARD domain of human CARMA1.
abstract: The CARMA1 signalosome, which is composed of CARMA1 [caspase recruitment domain (CARD) containing MAGUK protein 1], BCL10 (B-cell lymphoma 10) and MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1), is a molecular-signalling complex that performs pivotal functions in T-cell receptor (TCR) and B-cell receptor (BCR) mediated NF-κB activation. In this study, the CARD domain of human CARMA1 (CARMA1 CARD), corresponding to amino acids 14-109, was overexpressed in Escherichia coli using an engineered C-terminal His tag. CARMA1 CARD was then purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 3.2 Å from a crystal belonging to space group P2(1)2(1)2(1) with unit-cell parameters a = 45.73, b = 53.37, c = 91.89 Å.
authors.affiliation: School of Biotechnology and Graduate School of Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Park
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Park
mesh_terms: D053468:CARD Signaling Adaptor Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006162:Guanylate Cyclase / Q000737:chemistry*; D006801:Humans
chemical_list: D053468:CARD Signaling Adaptor Proteins; C426242:CARD11 protein, human; D006162:Guanylate Cyclase
keywords: CARD domains; CARMA1 signalosome; NF-κB
references.citation: Sci STKE. 2004 Jun 22;2004(239):re9
references.pmid: 15226512
references.citation: J Biol Chem. 2011 Nov 11;286(45):39528-36
references.pmid: 21880711
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Nat Immunol. 2008 Mar;9(3):272-81
references.pmid: 18264101
references.citation: Apoptosis. 2011 Mar;16(3):209-20
references.pmid: 21207148
references.citation: Annu Rev Immunol. 2007;25:561-86
references.pmid: 17201679
references.citation: Protein Expr Purif. 2004 Nov;38(1):1-8
references.pmid: 15477075
references.citation: Mol Cell. 2000 Oct;6(4):961-7
references.pmid: 11090634
references.citation: Nucleic Acids Res. 2012 Jan;40(Database issue):D331-6
references.pmid: 22135292
references.citation: J Biotechnol. 2011 Feb 20;151(4):335-42
references.pmid: 21237218
references.citation: EMBO J. 2007 Nov 14;26(22):4634-45
references.pmid: 17948050
references.citation: Nat Immunol. 2008 Mar;9(3):263-71
references.pmid: 18223652
references.citation: PLoS One. 2009;4(4):e4931
references.pmid: 19337385
references.citation: FEBS Lett. 2001 May 11;496(2-3):121-7
references.pmid: 11356195
references.citation: J Biol Chem. 2001 Apr 13;276(15):11877-82
references.pmid: 11278692
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: Nature. 2004 Jan 8;427(6970):167-71
references.pmid: 14695475
references.citation: J Biol Chem. 2001 Jun 1;276(22):19012-9
references.pmid: 11262391
references.citation: J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
references.pmid: 19461840
references.citation: Trends Biochem Sci. 2001 Aug;26(8):475-81
references.pmid: 11504623
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707