title: Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis-associated proliferative enteropathy in nursery pigs.
abstract: Quantitative polymerase chain reaction (qPCR) tests for detection and quantification of Lawsonia intracellularis in feces from pigs have been developed. The objective of the current study was to evaluate the diagnostic performance of a fecal qPCR test for detection of nursery pigs with L. intracellularis-associated proliferative enteropathy (PE) under field conditions. Furthermore, the diagnostic performance for different subpopulations of pigs was investigated, including pigs infected or noninfected with Porcine circovirus-2, Brachyspira pilosicoli, and Escherichia coli. The diagnostic performance was evaluated in terms of diagnostic sensitivity and specificity. Data from pigs originating from 20 herds with antibiotic treatment requiring diarrhea outbreaks from a prior study were reused. Before treatment, pigs were randomly selected for histological and immunohistochemical examination of intestinal segments and fecal quantification of L. intracellularis by qPCR. A total of 313 pigs (157 without diarrhea, 156 with diarrhea) were included in the statistical analysis, and 37 pigs (11.8%) were classified as PE positives (defined as proliferative histological lesions in combination with L. intracellularis demonstration by immunohistochemistry). Lawsonia intracellularis was detected by qPCR in feces from 91 pigs (29.1%). A nonparametric receiver operating characteristic (ROC) analysis provided an area under the ROC curve (0.93) and an optimal cutoff value of 4.8 log10 L. intracellularis bacteria/g feces. This cutoff provided a diagnostic sensitivity of 0.84 and diagnostic specificity of 0.93. The diagnostic sensitivity and specificity were significantly different between herds (P < 0.0001). Numerically, diagnostic sensitivity and specificity were different between subpopulations of pigs, but no significant differences were demonstrated.
authors.affiliation: HERD-Centre for Herd Oriented Education, Research and Development, Department of Large Animal Sciences, University of Copenhagen, Groennegaardsvej 2, DK-1870 Frederiksberg C, Denmark. ken@life.ku.dk
authors.forename: Ken Steen
authors.initials: KS
authors.lastname: Pedersen
authors.forename: Helle
authors.initials: H
authors.lastname: Stege
authors.forename: Tim K
authors.initials: TK
authors.lastname: Jensen
authors.forename: Roberto
authors.initials: R
authors.lastname: Guedes
authors.forename: Marie
authors.initials: M
authors.lastname: Ståhl
authors.forename: Jens Peter
authors.initials: JP
authors.lastname: Nielsen
authors.forename: Charlotte
authors.initials: C
authors.lastname: Hjulsager
authors.forename: Lars E
authors.initials: LE
authors.lastname: Larsen
authors.forename: Øystein
authors.initials: Ø
authors.lastname: Angen
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D045824:Desulfovibrionaceae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D004751:Enteritis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D020639:Lawsonia Bacteria; D012372:ROC Curve; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology
keywords: Feces; Lawsonia intracellularis; pigs; quantitative polymerase chain reaction; sensitivity; specificity