title: An HPLC method for microanalysis and pharmacokinetics of marine sulfated polysaccharide PSS-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles in rat plasma.
abstract: This study was aimed at developing a sensitive and selective HPLC method with postcolumn fluorescence derivatization for the detection of propylene glycol alginate sodium sulfate (PSS) in rat plasma. Plasma samples were prepared by a simple and fast ultrafiltration method. PSS was extracted from rat plasma with D-glucuronic acid as internal standard. Isocratic chromatographic separation was performed on a TSKgel G2500 PWxL column with the mobile phase of 0.1 M sodium sulfate at a flow rate of 0.5 mL/min. Analyte detection was achieved by fluorescence detection (FLD) at 250 nm (excitation) and 435 nm (emission) using guanidine hydrochloride as postcolumn derivatizing reagent in an alkaline medium at 120 °C. The calibration curve was linear over a concentration range of 1-500 μg/mL, and the lower limit of detection (LLOD) was found to be 250 ng/mL. This validated method was applied successfully to the pharmacokinetic study of PSS and PSS-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (PSS-NP) in rat plasma after a single intravenous (PSS only) and oral administration (PSS and PSS-NP). Significant differences in the main pharmacokinetic parameters of PSS and PSS-NP were observed. The relative bioavailability of PSS-NP was 190.10% compared with PSS which shows that PSS-NP can improve oral bioavailability.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. pengli.li@163.com
authors.forename: Peng-Li
authors.initials: PL
authors.lastname: Li
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Li
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Xue
authors.forename: Hai-Hua
authors.initials: HH
authors.lastname: Li
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Liu
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: He
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Yu
authors.forename: Hua-Shi
authors.initials: HS
authors.lastname: Guan
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005453:Fluorescence; D007275:Injections, Intravenous; D019344:Lactic Acid / Q000737:chemistry*; D057230:Limit of Detection; D008297:Male; D053758:Nanoparticles; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000737:chemistry
chemical_list: D000464:Alginates; D013431:Sulfates; C012036:sodium sulfate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; C038550:propylene glycol alginate ester; D019344:Lactic Acid
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