title: RNAi-mediated knockdown of catalase causes cell cycle arrest in SL-1 cells and results in low survival rate of Spodoptera litura (Fabricius).
abstract: Deregulated reactive oxygen species (ROS) production can lead to the disruption of structural and functional integrity of cells as a consequence of reactive interaction between ROS and various biological components. Catalase (CAT) is a common enzyme existing in nearly all organisms exposed to oxygen, which decomposes harmful hydrogen peroxide, into water and oxygen. In this study, the full length sequence that encodes CAT-like protein from Spodoptera litura named siltCAT (GenBank accession number: JQ_663444) was cloned and characterized. Amino acid sequence alignment showed siltCAT shared relatively high conservation with other insect, especially the conserved residues which defined heme and NADPH orientation. Expression pattern analysis showed that siltCAT mRNA was mainly expressed in the fat body, midgut, cuticle and malpighian tube, and as well as over last instar larvae, pupa and adult stages. RNA interference was used to silence CAT gene in SL-1 cells and the fourth-instar stage of S. litura larvae respectively. Our results provided evidence that CAT knockdown induced ROS generation, cell cycle arrest and apoptosis in SL-1 cells. It also confirmed the decrease in survival rate because of increased ROS production in experimental groups injected with double-stranded RNA of CAT (dsCAT). This study implied that ROS scavenging by CAT is important for S. litura survival.
authors.affiliation: Laboratory of Insect Toxicology, Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, People's Republic of China.
authors.forename: Haiming
authors.initials: H
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hu
authors.forename: Meiying
authors.initials: M
authors.lastname: Hu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.forename: Rizwan-ul-Haq
authors.initials: RU
authors.lastname: Muhammad
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Dong
authors.forename: Liang
authors.initials: L
authors.lastname: Gong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D002374:Catalase / Q000737:chemistry / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D055785:Gene Knockdown Techniques; D007300:Insect Control; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D017421:Sequence Analysis; D018411:Spodoptera / Q000166:cytology* / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D016019:Survival Analysis
chemical_list: D000975:Antioxidants; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D002374:Catalase
references.citation: Antioxid Redox Signal. 2006 Sep-Oct;8(9-10):1549-61
references.pmid: 16987010
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Genetics. 1989 Jul;122(3):643-52
references.pmid: 2503418
references.citation: Insect Biochem Mol Biol. 2005 Sep;35(9):961-79
references.pmid: 15978998
references.citation: Cell. 1990 Aug 24;62(4):671-80
references.pmid: 2143698
references.citation: J Mol Biol. 2000 Feb 11;296(1):295-309
references.pmid: 10656833
references.citation: Arch Insect Biochem Physiol. 2001 Oct;48(2):63-71
references.pmid: 11568965
references.citation: J Mol Biol. 2003 May 2;328(3):567-79
references.pmid: 12706717
references.citation: Arch Insect Biochem Physiol. 2008 Jul;68(3):134-43
references.pmid: 18454489
references.citation: J Biol Chem. 2009 Mar 6;284(10):6520-9
references.pmid: 19098005
references.citation: Arch Biochem Biophys. 2012 Sep 15;525(2):102-10
references.pmid: 22209752
references.citation: Science. 2005 Jun 24;308(5730):1909-11
references.pmid: 15879174
references.citation: Biochemistry. 1997 Aug 5;36(31):9356-64
references.pmid: 9235978
references.citation: Geochem Trans. 2005 Jun 14;6(3):47
references.pmid: 35412761
references.citation: Am J Respir Cell Mol Biol. 2003 Jun;28(6):705-12
references.pmid: 12600834
references.citation: Biochimie. 2002 Feb-Mar;84(2-3):131-41
references.pmid: 12022944
references.citation: Free Radic Biol Med. 2001 Aug 1;31(3):321-30
references.pmid: 11461769
references.citation: PLoS Pathog. 2007 Jun;3(6):e91
references.pmid: 17604451
references.citation: Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8
references.pmid: 3856839
references.citation: J Insect Physiol. 2009 Mar;55(3):273-8
references.pmid: 19135057
references.citation: Insect Biochem Mol Biol. 2001 Mar 15;31(4-5):393-400
references.pmid: 11222948
references.citation: PLoS One. 2012;7(2):e31425
references.pmid: 22363645
references.citation: Nat Biotechnol. 2007 Nov;25(11):1322-6
references.pmid: 17982443
references.citation: Nat Protoc. 2007;2(4):953-71
references.pmid: 17446895
references.citation: Science. 2010 Mar 26;327(5973):1644-8
references.pmid: 20223948
references.citation: Arch Insect Biochem Physiol. 2004 Feb;55(2):79-89
references.pmid: 14745825
references.citation: Appl Microbiol Biotechnol. 2010 May;86(5):1419-30
references.pmid: 19967355
references.citation: Toxicol Sci. 2011 Aug;122(2):557-66
references.pmid: 21622943
references.citation: Insect Biochem Mol Biol. 2005 Apr;35(4):277-83
references.pmid: 15763464
references.citation: Arch Biochem Biophys. 2002 May 15;401(2):145-54
references.pmid: 12054464
references.citation: Trends Biochem Sci. 1996 Mar;21(3):83-6
references.pmid: 8882579
references.citation: Subcell Biochem. 2007;44:295-315
references.pmid: 18084900
references.citation: PLoS One. 2011;6(5):e20504
references.pmid: 21655219
references.citation: J Biochem. 2010 Aug;148(2):189-200
references.pmid: 20495011
references.citation: Virology. 2001 Sep 1;287(2):391-404
references.pmid: 11531416
references.citation: J Neurosci. 2004 Feb 18;24(7):1531-40
references.pmid: 14973232
references.citation: Hepatology. 1997 Oct;26(4):935-43
references.pmid: 9328316
references.citation: J Biol Chem. 2000 Oct 27;275(43):33883-9
references.pmid: 10931831
references.citation: EMBO Rep. 2002 Sep;3(9):852-6
references.pmid: 12189180
references.citation: Biochem J. 1983 Nov 15;216(2):503-6
references.pmid: 6661212
references.citation: Free Radic Biol Med. 1990;8(4):401-13
references.pmid: 2199343
references.citation: Cell Prolif. 2003 Jun;36(3):165-75
references.pmid: 12814432
references.citation: Environ Toxicol Pharmacol. 2005 Nov;20(3):412-6
references.pmid: 21783620
references.citation: BMC Evol Biol. 2007 Jul 06;7:109
references.pmid: 17612411
references.citation: J Biol Chem. 2008 Feb 8;283(6):3217-3223
references.pmid: 18065421
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Curr Opin Cell Biol. 2006 Jun;18(3):240-6
references.pmid: 16631362
references.citation: Arch Insect Biochem Physiol. 1995;29(2):187-97
references.pmid: 7606043
references.citation: Pest Manag Sci. 2011 May;67(5):514-20
references.pmid: 21472969
references.citation: PLoS One. 2011 Mar 09;6(3):e17486
references.pmid: 21408075
references.citation: Methods Enzymol. 1984;105:121-6
references.pmid: 6727660
references.citation: Insect Biochem Mol Biol. 2006 Feb;36(2):154-60
references.pmid: 16431282
references.citation: J Cell Physiol. 1995 Nov;165(2):398-405
references.pmid: 7593218