title: Angiogenesis imaging in myocardial infarction using 68Ga-NOTA-RGD PET: characterization and application to therapeutic efficacy monitoring in rats.
abstract: OBJECTIVE
  Ga-NOTA-RGD PET is a newly developed molecular imaging for angiogenesis. In this study, Ga-NOTA-RGD PET was used to investigate imaging characteristics in a rat myocardial infarction (MI) model and to monitor the efficacy of an angiogenesis induction therapy.
  
  
  METHODS
  Ga-NOTA-RGD PET was performed serially in rats with MI or sham operation, and myocardial uptake was analyzed with respect to time duration and tissue characteristics. Subsequently, Ga-NOTA-RGD PET was serially performed for therapeutic efficacy monitoring in MI-induced rats, which were treated with basic fibroblast growth factor (bFGF) injection or saline injection. Image findings were compared with the final change in MI lesion.
  
  
  RESULTS
  Ga-NOTA-RGD uptake was significantly increased in MI lesion and gradually decreased over time. Ga-NOTA-RGD uptake in the infarcted tissue corresponded with vascular endothelial growth factor expression and macrophage accumulation. In monitoring of therapeutic efficacy, the lesion uptake in the bFGF-injected group was significantly higher than that of the saline-injected and sham-operated groups on the first day. However, no significant differences were observed between bFGF and saline-injected groups at subsequent time points, corresponding to the final infarct size change.
  
  
  CONCLUSIONS
  Ga-NOTA-RGD PET would be a useful angiogenesis imaging modality in MI for assessment of pathophysiology or monitoring of therapeutic efficacy.
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea.
authors.forename: Jae Seon
authors.initials: JS
authors.lastname: Eo
authors.forename: Jin Chul
authors.initials: JC
authors.lastname: Paeng
authors.forename: Song
authors.initials: S
authors.lastname: Lee
authors.forename: Yun-Sang
authors.initials: YS
authors.lastname: Lee
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Jeong
authors.forename: Keon Wook
authors.initials: KW
authors.lastname: Kang
authors.forename: June-Key
authors.initials: JK
authors.lastname: Chung
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D056831:Coordination Complexes; D004195:Disease Models, Animal; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005710:Gallium Radioisotopes; D006321:Heart / Q000000981:diagnostic imaging*; D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; D006801:Humans; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009842:Oligopeptides; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C584295:68Ga-1,4,7-triazacyclononane-1,4-7-triacetic acid-isothiocyanatobenzyl-c(Arg-Gly-Asp-Tyr-Lys); D043925:Angiogenesis Inducing Agents; D056831:Coordination Complexes; D005710:Gallium Radioisotopes; D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; D009842:Oligopeptides; D019275:Radiopharmaceuticals; D016222:Fibroblast Growth Factor 2; C048993:1,4,7-triazacyclononane-N,N',N''-triacetic acid; C047981:arginyl-glycyl-aspartic acid