title: Tenacibaculum halocynthiae sp. nov., a member of the family Flavobacteriaceae isolated from sea squirt Halocynthia roretzi.
abstract: A Gram-negative, non-spore-forming, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated P-R2A1-2(T), was isolated from sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. It grew optimally at 25-28 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that the strain fell within the clade comprising Tenacibaculum species. Strain P-R2A1-2(T) exhibited the highest 16S rRNA gene sequence similarity values of 97.6, 97.2 and 97.0 % to Tenacibaculum aestuarii SMK-4(T), T. lutimaris TF-26(T) and T. aiptasiae a4(T), respectively, and of 94.5-96.8 % to the type strains of the other Tenacibaculum species. Strain P-R2A1-2(T) contained MK-6 as the predominant menaquinone and C16:1 ω7c and/or iso-C15:0 2-OH, iso-C15:0 3-OH and iso-C15:0 as the major fatty acids. The DNA G + C content of strain P-R2A1-2(T) was 30.7 mol % and its DNA-DNA relatedness values with the type strains of T. aestuarii, T. lutimaris and T. aiptasiae were 17 ± 4.2, 21 ± 6.1 and 16 ± 5.2 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separate from other Tenacibaculum species. On the basis of the data presented, strain P-R2A1-2(T) is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum halocynthiae sp. nov. is proposed. The type strain is P-R2A1-2(T) (=KCTC 32262(T )= CCUG 63681(T)).
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Busan, Gijang, 619-705, South Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Jee
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids; D020459:Genes, rRNA; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D060493:Tenacibaculum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D014561:Urochordata / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S