title: Extraction optimization of Angelica sinensis polysaccharides and its antioxidant activity in vivo.
abstract: Extraction of Angelica sinensis polysaccharides (ASP) was optimized by the utilization of response surface methodology, RSM. Through the analysis, extraction time and water/solid were found to be the most significant factors. Based on contour plots and variance analysis, optimum operational conditions for maximizing polysaccharides yield (5.6%) were found to be extraction time 130 min, water/solid 5, and extraction number 5. A. sinensis polysaccharides (150 and 300 mg/kg) were administered for 15 days. The hepatoprotective activity was assessed using various biochemical parameters. Serum aspartate aminotransferase (AST), alanine aminotransfere (ALT) and alkaline phosphatase (ALP) levels were significantly restored toward normalization by the extracts (150 and 300 mg/kg body weight). ASP (150 and 300 mg/kg body weight) significantly increased the levels of antioxidant enzymes. It can be concluded that ASP possesses significant protective effect against hepatotoxicity induced by carbon tetrachloride (CCl4). This protective effect appears due to ASP antioxidant properties.
authors.affiliation: Medical college, DaLian University, DaLian City, 116600, PR China. fangyu1153@sina.com
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Huali
authors.initials: H
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Meng
authors.forename: Di
authors.initials: D
authors.lastname: Yang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005978:Glutathione / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D018384:Oxidative Stress; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D005978:Glutathione