title: Molecular-level dengue fever diagnostic devices made out of paper.
abstract: This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.
authors.affiliation: Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Shih-Jie
authors.initials: SJ
authors.lastname: Lo
authors.forename: Shih-Chun
authors.initials: SC
authors.lastname: Yang
authors.forename: Da-Jeng
authors.initials: DJ
authors.lastname: Yao
authors.forename: Jiann-Hwa
authors.initials: JH
authors.lastname: Chen
authors.forename: Wu-Chun
authors.initials: WC
authors.lastname: Tu
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Cheng
mesh_terms: D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D010209:Paper; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D012367:RNA, Viral