title: Expression of blaA underlies unexpected ampicillin-induced cell lysis of Shewanella oneidensis.
abstract: Shewanella oneidensis is a facultative anaerobic γ-proteobacterium possessing remarkably diverse respiratory capacities for reducing various organic and inorganic substrates. As a veteran research model for investigating redox transformations of environmental contaminants the bacterium is well known to be a naturally ampicillin-resistant microorganism. However, in this study we discovered that ampicillin has a significant impact on growth of S. oneidensis. Particularly, cell lysis occurred only with ampicillin at levels ranging from 0.49 to 6.25 µg/ml but not at 50 µg/ml. This phenotype is attributable to insufficient expression of the β-lactamase BlaA. The subsequent analysis revealed that the blaA gene is strongly induced by ampicillin at high (50 µg/ml), but not at low levels (2.5 µg/ml). In addition, we demonstrated that penicillin binding protein 5 (PBP5), the most abundant low molecular weight PBP (LMW PBP), is the only one relevant to β-lactam resistance under the tested conditions. This nonessential PBP, largely resembling its Escherichia coli counterpart in functionality, mediates expression of the blaA gene.
authors.affiliation: Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yin
authors.forename: Linlin
authors.initials: L
authors.lastname: Sun
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Dong
authors.forename: Xun
authors.initials: X
authors.lastname: Chi
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Qi
authors.forename: Haichun
authors.initials: H
authors.lastname: Gao
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy* / Q000382:microbiology; D006801:Humans; D046915:Penicillin-Binding Proteins / Q000378:metabolism; D010406:Penicillins / Q000494:pharmacology; D020592:Shewanella / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C074160:BlaA protein, Bacteria; D000667:Ampicillin
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