title: Current topics of immunohistochemical and biological properties of human lymphatic endothelial cells.
abstract: We examined the immunohistochemical properties of selective lymph vessel markers such as LYVE-1, podoplanin, Prox-1, and VEGF R3, as well as NO synthase (NOS) and cyclo-oxygenase (COX) in two kinds of human lymphatic endothelial cell isolated from collecting and initial lymph vessels. The constitutively expressed genes in the two kinds of lymphatic endothelial cell were also evaluated using oligonucleotide microarray analysis and RT-PCR. We also investigated the effects of the oxygen concentration in culture conditions on the proliferative activities of the two kinds of human lymphatic endothelial cell. Immunoreactivity to LYVE-1 and the RT-PCR expression level of LYVE-1 mRNA in endothelial cells of initial lymph vessels were stronger than those of collecting lymph vessels. Immunoreactivity to ecNOS, iNOS, COX1, and COX2 was also found to be significantly higher than in collecting lymph vessels. In contrast, an increase in the O2 concentration ranging from 5% to 21% caused a significant reduction in the proliferative activity of endothelial cells in collecting lymph vessels. In conclusion, these findings suggest that there exists a marked heterogeneity in the immunohistochemical, genomic, and proliferative activity of human lymphatic endothelial cells between initial and collecting lymph vessels. (*English Translation of J Jpn Coll Angiol, 2008, 48: 125-130.).
authors.affiliation: Department of Physiology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kawai
authors.forename: Toshio
authors.initials: T
authors.lastname: Ohhashi
keywords: collecting lymph vessel; culture; initial lymph vessel; lymphatic endothelial cell; sentinel lymph node
references.citation: Microcirculation. 2003 Apr;10(2):127-31
references.pmid: 12700581
references.citation: Pharmacol Ther. 2005 Feb;105(2):165-88
references.pmid: 15670625
references.citation: J Surg Oncol. 1997 Dec;66(4):282-4
references.pmid: 9425336
references.citation: Cancer Sci. 2012 Jul;103(7):1245-52
references.pmid: 22463128
references.citation: Jpn J Clin Oncol. 1999 Jan;29(1):11-5
references.pmid: 10073145
references.citation: Nature. 2005 Dec 8;438(7069):820-7
references.pmid: 16341007
references.citation: Lymphat Res Biol. 2008;6(1):15-27
references.pmid: 18361767
references.citation: Arch Surg. 1992 Apr;127(4):392-9
references.pmid: 1558490
references.citation: Microcirculation. 1999 Mar;6(1):75-8
references.pmid: 10100191
references.citation: J Cell Physiol. 2006 Apr;207(1):107-13
references.pmid: 16261591
references.citation: Nat Cell Biol. 2006 Dec;8(12):1369-75
references.pmid: 17128264
references.citation: Jpn J Physiol. 1998 Apr;48(2):133-41
references.pmid: 9639548
references.citation: Anat Rec. 1993 Aug;236(4):641-52
references.pmid: 8379589
references.citation: Lymphat Res Biol. 2007;5(2):115-26
references.pmid: 17935479