title: Anti-inflammatory activity of hexane extracts from bones and internal organs of Anguilla japonica suppresses cyclooxygenase-2-dependent prostaglandin D₂ generation in mast cells and anaphylaxis in mice.
abstract: The purpose of this study is to investigate the effects of n-hexane extracts from bones and internal organs of Japanese eel, Anguilla japonica (HEE), on cyclooxygenase-2 (COX-2)-dependent prostaglandin D₂(PGD₂) generation in stem cell factor (SCF), IL-10, plus LPS-induced mouse bone marrow-derived mast cells (BMMCs) and on passive cutaneous anaphylaxis (PCA) in mice. HEE suppressed SCF/IL-10/LPS-induced PGD₂ generation, and concomitantly reduced COX-2 protein expression dose-dependently. To understand the mechanistic basis for the inhibition of PGD₂ generation by HEE, we examined the effects of HEE on upstream signaling pathways essential for COX-2 induction. HEE was found to inhibit the translocation of nuclear factor-κB (NF-κB) p65 subunit to the nucleus and its DNA-binding ability through the inhibition of TAK1, IKK and IκB phosphorylation. Furthermore, HEE also attenuated mitogen-activated protein kinase (MAPK)-mediated regulation of DNA binding of activator protein-1 (AP-1). Moreover, oral administration of HEE inhibited anti-dinitrophenyl (DNP) IgE-induced PCA in a dose dependent manner. Taken together, the present study provides new insights into the anti-inflammatory activity of HEE, which could be a promising candidate to be used for an inflammatory therapy.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.forename: Min Kyun
authors.initials: MK
authors.lastname: Na
authors.forename: Seok-Jong
authors.initials: SJ
authors.lastname: Suh
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Geum Jin
authors.initials: GJ
authors.lastname: Kim
authors.forename: Guang Hsuan
authors.initials: GH
authors.lastname: Chao
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Jeong
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Young-Chae
authors.initials: YC
authors.lastname: Chang
authors.forename: Makoto
authors.initials: M
authors.lastname: Murakami
authors.forename: Wonku
authors.initials: W
authors.lastname: Kang
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
mesh_terms: D000707:Anaphylaxis / Q000188:drug therapy*; D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001842:Bone and Bones / Q000737:chemistry; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006586:Hexanes; D016753:Interleukin-10 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D015230:Prostaglandin D2 / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D000099151:MAP Kinase Kinase Kinase 7
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D045424:Complex Mixtures; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D006586:Hexanes; D016753:Interleukin-10; D008070:Lipopolysaccharides; D020930:MAP Kinase Kinase Kinases; D016328:NF-kappa B; D015230:Prostaglandin D2; D018808:Transcription Factor AP-1; D000099151:MAP Kinase Kinase Kinase 7; C556062:IL10 protein, mouse; C026385:n-hexane