title: Marinoscillum luteum sp. nov., isolated from marine sediment.
abstract: A novel strain, designated SJP7(T), was isolated from sediment of the Tofua Arc of the Tonga Trench. The 16S rRNA gene sequence of the isolate showed the highest similarity to that of Marinoscillum pacificum KCCM 42325(T) (95.9%). Phylogenetic analysis revealed that the isolate formed a distinct phyletic line with Marinoscillum pacificum KCCM 42325(T) and Marinoscillum furvescens LMG 13023(T) within the family Cytophagaceae. Cells of strain SJP7(T) were Gram-stain-negative and appeared as long rods that were motile by gliding. Growth was observed at 15-40 °C (optimum, 30 °C), at pH 5.5-9.0 (optimum, pH 7.5-8.0) and in the presence of 0.5-7.0% (w/v) NaCl (optimum, 2.5-3%). The major respiratory quinone was MK-7. The dominant fatty acids were summed feature 3 (comprising C(16:1)ω7c and/or C(16:1)ω6c), iso-C(15:0) and C(16:1)ω5c. The DNA G+C content was 43.5 mol%. These properties support the affiliation of strain SJP7(T) with the genus Marinoscillum. Further phenotypic differentiation of strain SJP7(T) from other species of the genus Marinoscillum was indicated by the results of physiological and biochemical tests. On the basis of evidence from our polyphasic taxonomic study, strain SJP7(T) represents a novel species of the genus Marinoscillum, for which the name Marinoscillum luteum sp. nov. is proposed. The type strain of Marinoscillum luteum is SJP7(T) ( =KCTC 23939(T) =NCAIM B02491(T)).
authors.affiliation: Department of Microbiology, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Cha
authors.affiliation: Department of Microbiology, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea.
authors.forename: Soo-Je
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Microbiology, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea.
authors.forename: So-Jeong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea.
authors.forename: Jong-Geol
authors.initials: JG
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea.
authors.forename: Man-Young
authors.initials: MY
authors.lastname: Jung
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
authors.affiliation: Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Kae Kyoung
authors.initials: KK
authors.lastname: Kwon
authors.affiliation: Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Department of Earth and Environmental Sciences, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea.
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Seo
authors.affiliation: Department of Microbiology, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7