title: A gene encoding a peptide with similarity to the plant IDA signaling peptide (AtIDA) is expressed most abundantly in the root-knot nematode (Meloidogyne incognita) soon after root infection.
abstract: Small peptides play important roles in intercellular signaling. Inflorescence deficient in abscission (ida) is an Arabidopsis mutant that does not abscise (shed) its flower petals. The IDA gene encodes a small, secreted peptide that putatively binds to two redundant receptor-like kinases (HAESA and HAESA-like2) that initiate a signal transduction pathway. We identified IDA-like (IDL) genes in the genomic sequence for Meloidogyne incognita and Meloidogyne hapla. No orthologous sequences were found in any other genus of nematodes. Transcript for both M. incognita and M. hapla IDLs were found in total RNA isolated from infected root systems of tomato, Solanum lycopersicum. Five and three prime RACE of RNA from M. incognita infected tomato roots revealed a sequence of 392 nt that includes a poly (A) tail of 39 nt. The open reading frame encodes a 47 aa protein with a putative 25 aa N-terminal signal peptide. Expression of MiIDL1 is very low in eggs and pre-parasitic J2 and rapidly increases in the first four days post inoculation (dpi) and then declines at approximately 14 dpi. A proposed role for the root-knot nematode IDL is discussed.
authors.affiliation: Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Dept. of Agriculture, Bldg 006, BARC-West, Beltsville, MD 20705, United States. mark.tucker@ars.usda.gov
authors.forename: Mark L
authors.initials: ML
authors.lastname: Tucker
authors.forename: Ronghui
authors.initials: R
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002212:Capsicum / Q000469:parasitology; D005786:Gene Expression Regulation / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D018551:Solanum lycopersicum / Q000254:growth & development / Q000469:parasitology*; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000469:parasitology*; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014431:Tylenchoidea / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C512213:IDA protein, Arabidopsis; D010445:Peptide Elongation Factors; D010940:Plant Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D018749:RNA, Plant; D014157:Transcription Factors