title: Analysis of nucleosides and nucleotides in infant formula by liquid chromatography-tandem mass spectrometry.
abstract: A method for the simultaneous analysis of nucleosides and nucleotides in infant formula using reversed-phase liquid chromatography-tandem mass spectrometry is described. This approach is advantageous for compliance testing of infant formula over other LC-MS methods in which only nucleotides or nucleosides are measured. Following sample dissolution, protein was removed by centrifugal ultrafiltration. Chromatographic analyses were performed using a C18 stationary phase and gradient elution of an ammonium acetate/bicarbonate buffer, mass spectrometric detection and quantitation by a stable isotope-labelled internal standard technique. A single laboratory validation was performed, with spike recoveries of 80.1-112.9% and repeatability relative standard deviations of 1.9-7.2%. Accuracy as bias was demonstrated against reference values for NIST1849a certified reference material. The method has been validated for the analysis of bovine milk-based, soy-based, caprine milk-based and hydrolysed milk protein-based infant formulae.
authors.affiliation: Fonterra Co-operative Group Ltd., P.O. Box 7, Waitoa 3380, New Zealand. brendon.gill@fonterra.com
authors.forename: Brendon D
authors.initials: BD
authors.lastname: Gill
authors.forename: Harvey E
authors.initials: HE
authors.lastname: Indyk
authors.forename: Merilyn
authors.initials: M
authors.lastname: Manley-Harris
mesh_terms: D000485:Allergens / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D006041:Goats; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D041942:Soy Milk / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000485:Allergens; D009705:Nucleosides; D009711:Nucleotides