title: Expression of interleukin (IL)-10, IL-17A and IL-22 in serum and sputum of stable chronic obstructive pulmonary disease patients.
abstract: Interleukin (IL)-17A, IL-22 and IL-10 have been implicated in the development of chronic obstructive pulmonary disease (COPD), but their expression in COPD is uncertain. Here we investigate the expression of IL-17A, IL-22 and IL-10 in the serum and sputum of COPD patients. Blood samples and induced sputum samples were collected from 94 patients with COPD, 23 healthy smokers, and 22 healthy control non-smokers. IL-17A, IL-22 and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). We found that: 1) serum and sputum IL-17A were higher in COPD compared to healthy smokers and non-smokers; 2) serum IL-17A increased with COPD stages, it was inversely correlated with percentage of forced expiratory volume in the first second (FEV1%) reference and positively correlated with C-reactive protein (CRP), Sputum IL-17A levels in the severe COPD patients were positively correlated with sputum neutrophils, and reversely correlated with sputum macraphages (p < 0.01); 3) serum and sputum IL-22 were significantly higher in COPD and healthy smokers than those in the non-smoker group, sputum IL-22 was similar in severe COPD (stage III and IV), which were higher than those in the other groups (p < 0.05); and, 4) serum and sputum IL-10 were similiar in COPD and healthy smokers, which were decreased compared to non-smokers. These data suggest that the increased level of IL-17A in serum and sputum plays important roles in the pathogenesis of COPD. The increased sputum IL-22 might also play important roles in the pathogenesis of COPD, while IL-10 secretion might be not only affected by COPD but also by cigarette smoke.
authors.affiliation: Department of Respiratory Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430070, P. R. China. zhangcatli1979@sina.com
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhenshun
authors.initials: Z
authors.lastname: Cheng
authors.forename: Weimin
authors.initials: W
authors.lastname: Liu
authors.forename: Kaisong
authors.initials: K
authors.lastname: Wu
mesh_terms: D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000378:metabolism*; D020381:Interleukin-17 / Q000097:blood / Q000378:metabolism*; D007378:Interleukins / Q000097:blood / Q000378:metabolism*; D008264:Macrophages; D008297:Male; D008875:Middle Aged; D009504:Neutrophils; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D012907:Smoking / Q000097:blood / Q000378:metabolism*; D013183:Sputum / Q000166:cytology / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D002097:C-Reactive Protein; D016753:Interleukin-10; D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22