title: Novel chitosan derivative for temperature and ultrasound dual-sensitive liposomal microbubble gel.
abstract: In this study, a novel liposome-loaded microbubble gel based on N-cholesteryl hemisuccinate-O-sulfate chitosan (NCHOSC) was designed. The structure of the NCHOSC was characterized by FTIR and (1)H NMR. The liposomal microbubble gel based on NCHOSC with a high encapsulation efficiency of curcumin was formed and improved the solubility of curcumin. The diameter of most liposomal microbubble was about 950 nm. The temperature-sensitive CS/GP gel could be formulated at room temperature and would form a gel at body temperature. Simultaneously, the ultrasound-sensitive induced release of curcumin was 85% applying ultrasound. The results of cytotoxicity assay indicated that encapsulated curcumin in Cur-LM or Cur-LM-G was less toxic. The anti-tumor efficacy in vivo suggested that Cur-LM-G by ultrasound suppressed tumor growth most efficiently. These findings have shed some light on the potential NCHOSC material used to liposome-loaded microbubble gel for temperature and ultrasound dual-sensitive drug delivery.
authors.affiliation: School of Pharmacy, Yantai University, Yantai, People's Republic of China. cdq1981@126.com
authors.forename: Daquan
authors.initials: D
authors.lastname: Chen
authors.forename: Hongyun
authors.initials: H
authors.lastname: Yu
authors.forename: Hongjie
authors.initials: H
authors.lastname: Mu
authors.forename: Junhua
authors.initials: J
authors.lastname: Wei
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Song
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
authors.forename: Rongcai
authors.initials: R
authors.lastname: Liang
authors.forename: Kaoxiang
authors.initials: K
authors.lastname: Sun
authors.forename: Wanhui
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D002788:Cholesterol Esters / Q000138:chemical synthesis / Q000737:chemistry*; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D004339:Drug Compounding / Q000379:methods; D004355:Drug Stability; D005260:Female; D005782:Gels; D006801:Humans; D008081:Liposomes; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D013016:Sound; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002214:Capsules; D002788:Cholesterol Esters; D003692:Delayed-Action Preparations; D005782:Gels; D008081:Liposomes; C583469:N-cholesteryl hemisuccinate-O-sulfate chitosan; D048271:Chitosan; D003474:Curcumin