title: Modulation of the enterohemorrhagic E. coli virulence program through the human gastrointestinal tract.
abstract: Enteric pathogens must not only survive passage through the gastrointestinal tract but must also coordinate expression of virulence determinants in response to localized microenvironments with the host. Enterohemorrhagic Escherichia coli (EHEC), a serious food and waterborne human pathogen, is well equipped with an arsenal of molecular factors that allows it to survive passage through the gastrointestinal tract and successfully colonize the large intestine. This review will explore how EHEC responds to various environmental cues associated with particular microenvironments within the host and how it employs these cues to modulate virulence factor expression, with a view to developing a conceptual framework for understanding modulation of EHEC's virulence program in response to the host. In vitro studies offer significant insights into the role of individual environmental cues but in vivo studies using animal models as well as data from natural infections will ultimately provide a more comprehensive picture of the highly regulated virulence program of this pathogen.
authors.affiliation: Department of Chemistry and Biology, Faculty of Science, Ryerson University, Toronto, ONT, Canada. dfoster@ryerson.ca
authors.forename: Debora
authors.initials: D
authors.lastname: Barnett Foster
mesh_terms: D000818:Animals; D054324:Enterohemorrhagic Escherichia coli / Q000472:pathogenicity* / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006801:Humans
keywords: colonization; enterohemorrhagic E. coli(EHEC); environment; gastrointestinal tract; virulence
references.citation: J Bacteriol. 2011 Sep;193(17):4509-15
references.pmid: 21725004
references.citation: Mol Microbiol. 2000 Nov;38(4):805-16
references.pmid: 11115115
references.citation: Cell Microbiol. 2008 Mar;10(3):549-56
references.pmid: 18053003
references.citation: BMC Med. 2012 Feb 02;10:12
references.pmid: 22300510
references.citation: mBio. 2012 Jul 03;3(4):e00172-12
references.pmid: 22761393
references.citation: J Biol Chem. 2008 Aug 29;283(35):24245-53
references.pmid: 18577510
references.citation: J Bacteriol. 2009 Mar;191(6):1868-77
references.pmid: 19114477
references.citation: Toxicon. 2012 Nov;60(6):1085-107
references.pmid: 22960449
references.citation: Cell Microbiol. 2007 Apr;9(4):1039-49
references.pmid: 17381433
references.citation: J Infect Dis. 1985 May;151(5):775-82
references.pmid: 3886804
references.citation: J Bacteriol. 2004 Jan;186(2):575-9
references.pmid: 14702327
references.citation: Infect Immun. 2001 Dec;69(12):7356-64
references.pmid: 11705908
references.citation: J Bacteriol. 2008 Apr;190(7):2286-97
references.pmid: 18223090
references.citation: Infect Immun. 2006 Apr;74(4):2233-44
references.pmid: 16552054
references.citation: Microbiology (Reading). 2011 Jun;157(Pt 6):1768-1775
references.pmid: 21454368
references.citation: Infect Immun. 2002 Jan;70(1):121-6
references.pmid: 11748172
references.citation: Curr Gastroenterol Rep. 2009 Aug;11(4):301-6
references.pmid: 19615306
references.citation: J Infect Dis. 2005 Oct 15;192(8):1430-40
references.pmid: 16170762
references.citation: Microbiology (Reading). 2009 Feb;155(Pt 2):521-530
references.pmid: 19202100
references.citation: Cell. 1997 Nov 14;91(4):511-20
references.pmid: 9390560
references.citation: J Clin Microbiol. 2003 Nov;41(11):4930-40
references.pmid: 14605120
references.citation: Infect Immun. 2011 Mar;79(3):1016-24
references.pmid: 21149585
references.citation: Infect Immun. 2005 Feb;73(2):965-71
references.pmid: 15664939
references.citation: Microb Pathog. 2008 Apr;44(4):271-8
references.pmid: 17997077
references.citation: Gut Microbes. 2012 Mar-Apr;3(2):104-20
references.pmid: 22356862
references.citation: Eur J Biochem. 1988 Jan 15;171(1-2):45-50
references.pmid: 3276522
references.citation: Infect Immun. 2005 Jan;73(1):18-29
references.pmid: 15618137
references.citation: J Bacteriol. 2003 Jan;185(1):204-9
references.pmid: 12486057
references.citation: Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9667-72
references.pmid: 16766659
references.citation: J Biol Chem. 1996 Aug 23;271(34):20359-64
references.pmid: 8702771
references.citation: FEMS Microbiol Rev. 2005 Nov;29(5):1021-40
references.pmid: 16023758
references.citation: Adv Appl Microbiol. 2011;75:111-43
references.pmid: 21807247
references.citation: PLoS Pathog. 2009 Aug;5(8):e1000553
references.pmid: 19696934
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10420-10425
references.pmid: 16803956
references.citation: Int J Med Microbiol. 2006 Aug;296(4-5):197-210
references.pmid: 16618552
references.citation: Infect Immun. 2002 Jul;70(7):3500-9
references.pmid: 12065489
references.citation: Environ Microbiol. 2010 Sep;12(9):2426-35
references.pmid: 20406285
references.citation: Pediatr Nephrol. 2011 Nov;26(11):2031-9
references.pmid: 21603905
references.citation: Microb Pathog. 1999 Nov;27(5):289-301
references.pmid: 10545256
references.citation: Pediatr Nephrol. 2008 Oct;23(10):1749-60
references.pmid: 18704506
references.citation: Mol Microbiol. 2006 Jan;59(1):193-201
references.pmid: 16359328
references.citation: J Appl Bacteriol. 1992 Jan;72(1):57-64
references.pmid: 1541601
references.citation: Am J Physiol. 1974 May;226(5):1043-50
references.pmid: 4824856
references.citation: J Bacteriol. 2012 Sep;194(18):5020-33
references.pmid: 22797761
references.citation: Infect Immun. 2005 Mar;73(3):1466-74
references.pmid: 15731044
references.citation: Mol Microbiol. 2003 Jun;48(6):1633-45
references.pmid: 12791144
references.citation: N Engl J Med. 2000 Jun 29;342(26):1930-6
references.pmid: 10874060
references.citation: Nat Rev Microbiol. 2004 Nov;2(11):898-907
references.pmid: 15494746
references.citation: Fish Shellfish Immunol. 2011 Mar;30(3):944-53
references.pmid: 21288493
references.citation: Infect Immun. 1996 Mar;64(3):966-73
references.pmid: 8641808
references.citation: Infect Immun. 2009 Feb;77(2):783-90
references.pmid: 19064636
references.citation: Microbiology (Reading). 2004 Jul;150(Pt 7):2037-2053
references.pmid: 15256548
references.citation: Infect Immun. 1994 Aug;62(8):3494-505
references.pmid: 7518809
references.citation: Clin Microbiol Rev. 1998 Jan;11(1):142-201
references.pmid: 9457432
references.citation: FEMS Microbiol Lett. 2009 Jun;295(1):96-102
references.pmid: 19473256
references.citation: Front Cell Infect Microbiol. 2012 Jul 12;2:90
references.pmid: 22919681
references.citation: Expert Rev Anti Infect Ther. 2009 Aug;7(6):697-707
references.pmid: 19681698
references.citation: Microbiology (Reading). 2012 Sep;158(Pt 9):2399-2407
references.pmid: 22767547
references.citation: J Bacteriol. 2009 Jan;191(1):411-21
references.pmid: 18952791
references.citation: J Bacteriol. 1999 Apr;181(7):2257-60
references.pmid: 10094706
references.citation: Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839-43
references.pmid: 2172966
references.citation: Proc Natl Acad Sci U S A. 2010 May 25;107(21):9831-6
references.pmid: 20457895
references.citation: Infection. 2013 Jun;41(3):669-73
references.pmid: 23292662
references.citation: BMJ. 2012 Jul 19;345:e4565
references.pmid: 22815429
references.citation: J Clin Gastroenterol. 2006 Mar;40(3):235-43
references.pmid: 16633129
references.citation: Infect Immun. 1999 Apr;67(4):1614-22
references.pmid: 10084994
references.citation: J Biol Chem. 1987 Jun 25;262(18):8834-9
references.pmid: 3298243
references.citation: Infect Immun. 2001 Nov;69(11):6573-9
references.pmid: 11598024
references.citation: Clin Microbiol Rev. 2006 Apr;19(2):315-37
references.pmid: 16614252
references.citation: Infect Immun. 2002 Jun;70(6):3085-93
references.pmid: 12011002
references.citation: Lancet. 2005 Mar 19-25;365(9464):1073-86
references.pmid: 15781103
references.citation: N Engl J Med. 2000 Jun 29;342(26):1990-1
references.pmid: 10874069
references.citation: Mol Microbiol. 2003 Oct;50(1):205-17
references.pmid: 14507375
references.citation: Infect Immun. 2004 Dec;72(12):6764-72
references.pmid: 15557596
references.citation: Biochem Biophys Res Commun. 1988 Apr 29;152(2):674-9
references.pmid: 3284526
references.citation: JAMA. 2002 Aug 28;288(8):996-1001
references.pmid: 12190370
references.citation: BMC Microbiol. 2012 Aug 01;12:160
references.pmid: 22853739
references.citation: Microbiology (Reading). 2009 Nov;155(Pt 11):3554-3563
references.pmid: 19684070
references.citation: Expert Rev Anti Infect Ther. 2010 Nov;8(11):1225-9
references.pmid: 21073287
references.citation: J Microbiol Methods. 2008 May;73(2):125-32
references.pmid: 18328583
references.citation: Microbiology (Reading). 2009 Sep;155(Pt 9):2907-2918
references.pmid: 19497950
references.citation: FEMS Immunol Med Microbiol. 2004 Jun 1;41(2):177-85
references.pmid: 15145463
references.citation: Curr Top Microbiol Immunol. 1992;180:65-94
references.pmid: 1324134
references.citation: J Bacteriol. 2007 Oct;189(20):7426-35
references.pmid: 17693516
references.citation: Infect Immun. 1995 Sep;63(9):3739-44
references.pmid: 7642319
references.citation: Pediatrics. 2005 Jun;115(6):e673-80
references.pmid: 15930195
references.citation: Front Cell Infect Microbiol. 2012 Jun 07;2:81
references.pmid: 22919672
references.citation: Front Cell Infect Microbiol. 2012 Nov 15;2:138
references.pmid: 23162800
references.citation: Mol Microbiol. 2002 May;44(4):957-70
references.pmid: 12010491
references.citation: Gut. 1987 Oct;28(10):1221-7
references.pmid: 3678950
references.citation: Mol Biotechnol. 2004 Feb;26(2):117-22
references.pmid: 14764937
references.citation: Appl Environ Microbiol. 1996 Sep;62(9):3094-100
references.pmid: 8795195
references.citation: J Infect Dis. 2007 May 1;195(9):1353-60
references.pmid: 17397007
references.citation: Dig Dis Sci. 2000 Nov;45(11):2108-14
references.pmid: 11215723
references.citation: J Bacteriol. 1997 Apr;179(8):2512-8
references.pmid: 9098046
references.citation: Environ Microbiol. 2006 Jun;8(6):1033-47
references.pmid: 16689724
references.citation: Infect Immun. 2012 Feb;80(2):688-703
references.pmid: 22144490
references.citation: Infect Immun. 2000 Jun;68(6):3108-15
references.pmid: 10816451
references.citation: Mol Microbiol. 2006 Jul;61(1):194-205
references.pmid: 16824105
references.citation: Infect Dis Clin North Am. 1998 Mar;12(1):199-216
references.pmid: 9494839
references.citation: Appl Environ Microbiol. 2004 Aug;70(8):4792-9
references.pmid: 15294816
references.citation: Mol Gen Genet. 1999 Dec;262(4-5):600-7
references.pmid: 10628842
references.citation: Curr Opin Gastroenterol. 2008 Jan;24(1):38-47
references.pmid: 18043231
references.citation: Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10637-42
references.pmid: 17563352
references.citation: Vet Microbiol. 2011 Nov 21;153(1-2):2-12
references.pmid: 21592684
references.citation: J Bacteriol. 2007 Jul;189(14):5302-13
references.pmid: 17496091
references.citation: Mol Microbiol. 2007 Mar;63(5):1468-81
references.pmid: 17302821
references.citation: J Clin Invest. 2007 Nov;117(11):3519-29
references.pmid: 17948128
references.citation: Adv Exp Med Biol. 1997;412:331-9
references.pmid: 9192038
references.citation: Microb Pathog. 2007 Aug-Sep;43(2-3):78-87
references.pmid: 17524609
references.citation: Appl Environ Microbiol. 2008 Apr;74(7):2153-60
references.pmid: 18245257
references.citation: FEMS Microbiol Lett. 1996 Jan 15;135(2-3):161-7
references.pmid: 8595853
references.citation: Nature. 2010 May 20;465(7296):355-8
references.pmid: 20436458
references.citation: Clin Infect Dis. 2012 Jul;55(1):33-41
references.pmid: 22431799
references.citation: Infect Immun. 2001 Nov;69(11):6660-9
references.pmid: 11598035
references.citation: Infect Immun. 2007 Oct;75(10):4891-9
references.pmid: 17698572
references.citation: mBio. 2012 May 15;3(3):
references.pmid: 22589288
references.citation: Nat Med. 2011 Jul 07;17(7):755
references.pmid: 21738132
references.citation: Microbes Infect. 2000 Jul;2(8):907-13
references.pmid: 10962274
references.citation: Infect Immun. 2001 Jul;69(7):4447-57
references.pmid: 11401985
references.citation: Infect Immun. 2011 Apr;79(4):1696-705
references.pmid: 21245267
references.citation: Infect Immun. 2000 Dec;68(12):6763-9
references.pmid: 11083793
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14941-6
references.pmid: 16990433
references.citation: Pediatr Res. 2011 May;69(5 Pt 1):390-4
references.pmid: 21270676
references.citation: Microbiology (Reading). 2004 Apr;150(Pt 4):775-783
references.pmid: 15073288
references.citation: PLoS One. 2009;4(3):e4889
references.pmid: 19293938
references.citation: J Bacteriol. 2007 Mar;189(6):2468-76
references.pmid: 17220220
references.citation: Lancet. 1983 Mar 26;1(8326 Pt 1):702
references.pmid: 6132054
references.citation: Microbiology (Reading). 2005 Dec;151(Pt 12):4063-4070
references.pmid: 16339951
references.citation: Toxicol Lett. 1999 Mar 8;105(1):47-57
references.pmid: 10092056
references.citation: J Infect Dis. 2001 Apr 1;183(7):1063-70
references.pmid: 11237831
references.citation: J Biol Chem. 2002 Jan 25;277(4):2876-85
references.pmid: 11704679
references.citation: J Bacteriol. 2008 Nov;190(22):7441-52
references.pmid: 18776020