title: Cerebellar output in zebrafish: an analysis of spatial patterns and topography in eurydendroid cell projections.
abstract: The cerebellum is a brain region responsible for motor coordination and for refining motor programs. While a great deal is known about the structure and connectivity of the mammalian cerebellum, fundamental questions regarding its function in behavior remain unanswered. Recently, the zebrafish has emerged as a useful model organism for cerebellar studies, owing in part to the similarity in cerebellar circuits between zebrafish and mammals. While the cell types composing their cerebellar cortical circuits are generally conserved with mammals, zebrafish lack deep cerebellar nuclei, and instead a majority of cerebellar output comes from a single type of neuron: the eurydendroid cell. To describe spatial patterns of cerebellar output in zebrafish, we have used genetic techniques to label and trace eurydendroid cells individually and en masse. We have found that cerebellar output targets the thalamus and optic tectum, and have confirmed the presence of pre-synaptic terminals from eurydendroid cells in these structures using a synaptically targeted GFP. By observing individual eurydendroid cells, we have shown that different medial-lateral regions of the cerebellum have eurydendroid cells projecting to different targets. Finally, we found topographic organization in the connectivity between the cerebellum and the optic tectum, where more medial eurydendroid cells project to the rostral tectum while lateral cells project to the caudal tectum. These findings indicate that there is spatial logic underpinning cerebellar output in zebrafish with likely implications for cerebellar function.
authors.affiliation: School of Biomedical Sciences, The University of Queensland Brisbane, QLD, Australia.
authors.forename: Lucy A
authors.initials: LA
authors.lastname: Heap
authors.forename: Chi Ching
authors.initials: CC
authors.lastname: Goh
authors.forename: Karin S
authors.initials: KS
authors.lastname: Kassahn
authors.forename: Ethan K
authors.initials: EK
authors.lastname: Scott
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002525:Cerebellar Cortex / Q000166:cytology* / Q000502:physiology*; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D013477:Superior Colliculi / Q000166:cytology* / Q000502:physiology*; D015027:Zebrafish
keywords: Gal4; cerebellum; eurydendroid; optic tectum; thalamus; topography; zebrafish
references.citation: Acta Morphol Hung. 1983;31(1-3):219-34
references.pmid: 6414258
references.citation: J Comp Neurol. 2007 Jul 10;503(2):348-70
references.pmid: 17492628
references.citation: Nature. 1982 Sep 16;299(5880):248-9
references.pmid: 7110344
references.citation: PLoS One. 2012;7(2):e32295
references.pmid: 22359680
references.citation: Bioinformatics. 2011 Sep 1;27(17):2453-4
references.pmid: 21727141
references.citation: Development. 2005 Jul;132(13):2955-67
references.pmid: 15930106
references.citation: J Morphol. 1982 Dec;174(3):335-348
references.pmid: 30086606
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10357-62
references.pmid: 16002470
references.citation: J Microsc. 2009 Jan;233(1):42-60
references.pmid: 19196411
references.citation: J Neurosci. 2007 May 16;27(20):5271-9
references.pmid: 17507550
references.citation: Brain Behav Evol. 1988;32(5):293-316
references.pmid: 3233488
references.citation: Nature. 2007 Nov 1;450(7166):56-62
references.pmid: 17972876
references.citation: J Comp Neurol. 2004 Jul 19;475(2):143-62
references.pmid: 15211457
references.citation: Proc Natl Acad Sci U S A. 2010 May 4;107(18):8410-5
references.pmid: 20395550
references.citation: Cerebellum. 2006;5(4):268-74
references.pmid: 17134989
references.citation: Methods Cell Biol. 2004;77:201-22
references.pmid: 15602913
references.citation: Nat Neurosci. 1998 Nov;1(7):635-40
references.pmid: 10196573
references.citation: J Neurosci. 2011 Jun 15;31(24):8708-12
references.pmid: 21677154
references.citation: Front Neurosci. 2012 May 07;6:64
references.pmid: 22586363
references.citation: BMC Biol. 2010 Sep 28;8:126
references.pmid: 20920150
references.citation: Mech Dev. 1999 Feb;80(2):153-8
references.pmid: 10072782
references.citation: J Comp Neurol. 1982 Feb 1;204(4):325-35
references.pmid: 7061736
references.citation: Methods. 2006 Jul;39(3):199-206
references.pmid: 16814563
references.citation: Brain Behav Evol. 2002;60(1):36-51
references.pmid: 12239469
references.citation: J Comp Neurol. 2006 Feb 20;494(6):903-43
references.pmid: 16385483
references.citation: Dev Biol. 2008 Jun 1;318(1):162-71
references.pmid: 18423594
references.citation: Curr Biol. 2010 Apr 27;20(8):R371-81
references.pmid: 21749961
references.citation: Pflugers Arch. 1986 Feb;406(2):151-7
references.pmid: 3960698
references.citation: Neuroscience. 1982 Oct;7(10):2407-26
references.pmid: 7177381
references.citation: Exp Brain Res. 1982;45(1-2):253-64
references.pmid: 7056331
references.citation: Dev Dyn. 2007 Nov;236(11):3088-99
references.pmid: 17937395
references.citation: Methods. 2006 Jul;39(3):189-98
references.pmid: 16887366
references.citation: J Comp Neurol. 2006 Dec 1;499(4):546-64
references.pmid: 17029270
references.citation: J Physiol. 1967 Apr;189(2):261-79
references.pmid: 5340538
references.citation: J Neurophysiol. 1987 Jan;57(1):35-55
references.pmid: 3559680
references.citation: Prog Brain Res. 2012;196:145-62
references.pmid: 22341325
references.citation: Nat Methods. 2007 Apr;4(4):323-6
references.pmid: 17369834
references.citation: Brain Struct Funct. 2008 Aug;212(6):443-63
references.pmid: 18193279
references.citation: Dev Biol. 2007 Apr 15;304(2):811-24
references.pmid: 17335798
references.citation: J Physiol. 1966 Dec;187(3):553-74
references.pmid: 16783911
references.citation: Neuroscience. 1986 May;18(1):105-20
references.pmid: 2426627
references.citation: J Mot Behav. 1993 Sep;25(3):203-16
references.pmid: 12581990
references.citation: Nat Commun. 2012 Jun 26;3:924
references.pmid: 22735452
references.citation: Dev Biol. 2010 Jul 1;343(1-2):1-17
references.pmid: 20388506
references.citation: J Neurophysiol. 1968 Sep;31(5):785-97
references.pmid: 4974877
references.citation: J Comp Neurol. 2008 Jul 1;509(1):1-22
references.pmid: 18418897
references.citation: Nature. 2012 May 09;485(7399):471-7
references.pmid: 22622571
references.citation: PLoS One. 2010 Jul 09;5(7):e11503
references.pmid: 20634890
references.citation: Neuroscience. 1984 Feb;11(2):497-507
references.pmid: 6201783
references.citation: Development. 1999 Sep;126(17):3757-67
references.pmid: 10433906
references.citation: J Inherit Metab Dis. 2006 Feb;29(1):143-56
references.pmid: 16601881
references.citation: Cell Tissue Res. 2001 Sep;305(3):299-305
references.pmid: 11572083
references.citation: Arch Psychiatr Nervenkr (1970). 1971;215(1):92-106
references.pmid: 4333691
references.citation: Brain Behav Evol. 1988;31(5):269-82
references.pmid: 3395836
references.citation: Science. 2010 Oct 29;330(6004):669-73
references.pmid: 21030657
references.citation: J Neurochem. 2009 Jul;110(2):441-56
references.pmid: 19457087
references.citation: Development. 1993 Jun;118(2):401-15
references.pmid: 8223268
references.citation: Nature. 2009 Sep 17;461(7262):407-10
references.pmid: 19759620
references.citation: Proc Natl Acad Sci U S A. 2008 Jan 29;105(4):1255-60
references.pmid: 18202183
references.citation: Cortex. 2011 Jan;47(1):59-80
references.pmid: 19833328
references.citation: J Comp Neurol. 1994 Nov 8;349(2):165-81
references.pmid: 7860776
references.citation: J Neurosci. 1988 Dec;8(12):4513-30
references.pmid: 2848935
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13365-70
references.pmid: 19628697
references.citation: Genome Biol. 2006;7 Suppl 1:S12.1-14
references.pmid: 16925834
references.citation: Front Neural Circuits. 2009 Oct 09;3:13
references.pmid: 19862330
references.citation: J Comp Neurol. 1986 Mar 15;245(3):342-58
references.pmid: 2870092
references.citation: J Comp Neurol. 2006 Aug 1;497(4):542-65
references.pmid: 16739164
references.citation: Cell Tissue Res. 1983;231(1):55-72
references.pmid: 6850798
references.citation: J Neurophysiol. 1987 Jan;57(1):22-34
references.pmid: 3559673
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12651-6
references.pmid: 12271129
references.citation: J Comp Neurol. 1986 Mar 15;245(3):319-41
references.pmid: 3958249
references.citation: Curr Opin Neurobiol. 2011 Aug;21(4):609-15
references.pmid: 21767944
references.citation: Dev Neurobiol. 2012 Mar;72(3):282-301
references.pmid: 21309081
references.citation: J Neurosci. 2006 Mar 29;26(13):3604-14
references.pmid: 16571769
references.citation: Neuron. 2008 Mar 13;57(5):634-60
references.pmid: 18341986
references.citation: J Neurophysiol. 2007 Jul;98(1):54-62
references.pmid: 17507504
references.citation: Annu Rev Neurosci. 1981;4:273-99
references.pmid: 6784658
references.citation: Biol Lett. 2009 Feb 23;5(1):117-21
references.pmid: 18832057
references.citation: Neuron. 2000 Oct;28(1):41-51
references.pmid: 11086982
references.citation: Dev Biol. 2009 Jun 15;330(2):406-26
references.pmid: 19371731
references.citation: J Comp Neurol. 2013 Apr 15;521(6):1409-29
references.pmid: 23124714
references.citation: Nat Neurosci. 2004 Mar;7(3):254-60
references.pmid: 14758365
references.citation: Rev Oculomot Res. 1989;3:213-55
references.pmid: 2486324
references.citation: Hum Mol Genet. 2010 Apr 15;19(8):1438-52
references.pmid: 20089533
references.citation: J Comp Neurol. 1987 Feb 22;256(4):607-23
references.pmid: 3558892