title: Experimental designs for array comparative genomic hybridization technology.
abstract: Array comparative genomic hybridization (aCGH) technology is commonly used to estimate genome-wide copy-number variation and to evaluate associations between copy number and disease. Although aCGH technology is well developed and there are numerous algorithms available for estimating copy number, little attention has been paid to the important issue of the statistical experimental design. Herein, we review classical statistical experimental designs and discuss their relevance to aCGH technology as well as their importance for downstream statistical analyses. Furthermore, we provide experimental design guidance for various study objectives.
authors.affiliation: Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minn 55905, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: McDonnell
authors.forename: S M
authors.initials: SM
authors.lastname: Riska
authors.forename: E W
authors.initials: EW
authors.lastname: Klee
authors.forename: E C
authors.initials: EC
authors.lastname: Thorland
authors.forename: N E
authors.initials: NE
authors.lastname: Kay
authors.forename: S N
authors.initials: SN
authors.lastname: Thibodeau
authors.forename: A S
authors.initials: AS
authors.lastname: Parker
authors.forename: J E
authors.initials: JE
authors.lastname: Eckel-Passow
mesh_terms: D001667:Binding, Competitive; D002877:Chromosomes, Human / Q000235:genetics; D055028:Comparative Genomic Hybridization / Q000379:methods*; D056915:DNA Copy Number Variations; D015342:DNA Probes / Q000235:genetics; D056726:Genetic Association Studies; D030342:Genetic Diseases, Inborn / Q000175:diagnosis / Q000235:genetics; D005828:Genetics, Population / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes
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