title: A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia.
abstract: There is no valid and reliable diagnostic test for early diagnosis of acute mesenteric ischemia (AMI). The aim of this study was to measure the plasma levels of diamine oxidase (DAO) and citrulline in AMI to gain insight into its early diagnosis.
  
  
  
  A total of 21 Wistar albino rats were divided into three groups, that is, control group, short-term ischemia group, and prolonged ischemia group. The superior mesenteric artery was occluded for 15 min in the short-term ischemia group and for 12 h in the prolonged ischemia group. Twelve hours later, the experiment was terminated and plasma DAO and citrulline levels were measured. Intestinal tissue was evaluated for the histopathological changes.
  
  
  
  Compared to the control group, the short-term and prolonged ischemia groups showed significant increases in the plasma levels of DAO, whereas the plasma citrulline levels decreased significantly. Prolonged ischemia caused a larger increase in the plasma DAO levels and a larger decrease in the plasma citrulline levels compared to the short-term ischemia (p=0.011 and p=0.021, respectively). Intestinal damage was shown to develop more in the prolonged ischemia group (p=0.001).
  
  
  
  In the early period of AMI, the plasma DAO levels increase while citrulline levels decrease, and the extent of these changes depends on the duration of ischemia.
authors.affiliation: Department of General Surgery, State Hospital, Adıyaman, Turkey.
authors.forename: Rıdvan
authors.initials: R
authors.lastname: Cakmaz
authors.forename: Oktay
authors.initials: O
authors.lastname: Büyükaşık
authors.forename: Nurettin
authors.initials: N
authors.lastname: Kahramansoy
authors.forename: Hayri
authors.initials: H
authors.lastname: Erkol
authors.forename: Cavit
authors.initials: C
authors.lastname: Cöl
authors.forename: Cetin
authors.initials: C
authors.lastname: Boran
authors.forename: Güler
authors.initials: G
authors.lastname: Buğdaycı
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002956:Citrulline / Q000097:blood*; D003404:Creatinine / Q000097:blood; D042241:Early Diagnosis; D005260:Female; D007422:Intestines / Q000098:blood supply / Q000473:pathology*; D007511:Ischemia / Q000097:blood / Q000175:diagnosis*; D017538:Mesenteric Artery, Superior; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014508:Urea / Q000097:blood; D014652:Vascular Diseases / Q000097:blood / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D002956:Citrulline; D014508:Urea; D003404:Creatinine; D006631:Amine Oxidase (Copper-Containing)
keywords: animal model; citrulline; diamine oxidase; experimental; intestines
references.citation: Shock. 2012 Oct;38(4):411-9
references.pmid: 22777122
references.citation: J Clin Invest. 1980 May;65(5):1162-73
references.pmid: 7364943
references.citation: Semin Vasc Surg. 2010 Mar;23(1):4-8
references.pmid: 20298944
references.citation: Eur J Cardiothorac Surg. 2004 Feb;25(2):275-80
references.pmid: 14747126
references.citation: J Thromb Thrombolysis. 2012 May;33(4):355-61
references.pmid: 22081293
references.citation: J Clin Gastroenterol. 2012 Apr;46(4):308-10
references.pmid: 21897281
references.citation: Hepatogastroenterology. 2012 Jan-Feb;59(113):155-8
references.pmid: 22024144
references.citation: Dig Dis Sci. 1993 Jul;38(7):1195-200
references.pmid: 8325182
references.citation: Amino Acids. 2005 Nov;29(3):177-205
references.pmid: 16082501
references.citation: Dig Dis. 1994 Jan-Feb;12(1):2-14
references.pmid: 8200121
references.citation: Enzyme. 1984;31(4):217-20
references.pmid: 6432527
references.citation: J Surg Res. 1995 Aug;59(2):311-20
references.pmid: 7637347
references.citation: J Emerg Med. 2012 Jun;42(6):635-41
references.pmid: 22137151
references.citation: J Surg Res. 1991 Mar;50(3):228-33
references.pmid: 1900339
references.citation: J Mol Histol. 2012 Apr;43(2):171-8
references.pmid: 22350813
references.citation: Am J Surg. 2009 Apr;197(4):429-33
references.pmid: 19324109
references.citation: Dig Surg. 2012;29(3):226-35
references.pmid: 22699523
references.citation: Shock. 2012 Mar;37(3):297-305
references.pmid: 22089198
references.citation: Mol Med Rep. 2011 Nov-Dec;4(6):1095-9
references.pmid: 21842126
references.citation: Acta Oncol. 2011 Nov;50(8):1167-74
references.pmid: 21864050
references.citation: World J Surg. 2006 Aug;30(8):1579-85
references.pmid: 16865320
references.citation: Clin Nutr. 2008 Jun;27(3):328-39
references.pmid: 18440672
references.citation: Aliment Pharmacol Ther. 2008 May;27(10):980-7
references.pmid: 18315578
references.citation: J Burn Care Res. 2012 Nov-Dec;33(6):e316-8
references.pmid: 22878489
references.citation: Am Surg. 2011 Jul;77(7):832-8
references.pmid: 21944343
references.citation: J Clin Invest. 1980 Jul;66(1):66-70
references.pmid: 6772669
references.citation: J Surg Res. 2005 Aug;127(2):177-82
references.pmid: 15921697
references.citation: Anesthesiology. 2012 May;116(5):1035-46
references.pmid: 22417965
references.citation: Ulus Travma Acil Cerrahi Derg. 2011 May;17(3):193-8
references.pmid: 21935794
references.citation: Int J Surg. 2012;10(9):510-3
references.pmid: 22885139
references.citation: J Pediatr Surg. 2009 May;44(5):928-32
references.pmid: 19433172
references.citation: Gastroenterology. 1981 Feb;80(2):349-55
references.pmid: 6778762
references.citation: Nutr Clin Pract. 2012 Aug;27(4):527-32
references.pmid: 22706681
references.citation: Am J Transplant. 2012 Dec;12 Suppl 4:S27-32
references.pmid: 22812705
references.citation: Surgery. 2011 Oct;150(4):779-87
references.pmid: 22000191
references.citation: Intensive Care Med. 2011 Jun;37(6):911-7
references.pmid: 21400011
references.citation: Indian J Pharmacol. 2012 Jan;44(1):31-5
references.pmid: 22345866
references.citation: J Pediatr Surg. 2005 Mar;40(3):484-8
references.pmid: 15793722
references.citation: Pancreas. 2010 Jul;39(5):633-8
references.pmid: 20575163
references.citation: J Surg Res. 2009 Aug;155(2):210-6
references.pmid: 19505699
references.citation: Ann Oncol. 2010 Aug;21(8):1706-1711
references.pmid: 20089560
references.citation: Transplant Proc. 2002 May;34(3):918-20
references.pmid: 12034238
references.citation: Surgery. 2009 Jan;145(1):48-56
references.pmid: 19081475
references.citation: Ulus Travma Acil Cerrahi Derg. 2010 Jan;16(1):63-70
references.pmid: 20209399