title: Mass spectrometric detection of multiple extended series of neutral highly fucosylatedN-acetyllactosamine oligosaccharides in human milk.
abstract: Complex mixtures of high molecular weight fractions of pooled neutral human milk oligosaccharides (obtained via gel permeation chromatography) have been investigated. The subfractions were each permethylated and analyzed by high-resolution mass spectrometry, using matrix-assisted laser desorption/ionization (MALDI)-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry, in order to investigate their oligosaccharide compositions. The obtained spectra reveal that human milk contains more complex neutral oligosaccharides than have been described previously; the data show that these oligosaccharides can be highly fucosylated, and that their poly-N-acetyllactosamine cores are substituted with up to 10 fucose residues on a an oligosaccharide that has 7-N-acetyllactosamine units. This is the first report of the existence in human milk of this large range of highly fucosylated oligosaccharides which possess novel, potentially immunologically active structures.
authors.affiliation: Center for Biomedical Mass Spectrometry, Depts. of Biochemistry and Biophysics, Boston University School of Medicine, 670 Albany Street, Room 511, Boston, MA 02118-2646, USA ; Inst. für Pharmazeutische Chemie, JW Goethe Univ. of Frankfurt, Marie-Curie-Str. 9-11, Frankfurt a. Main 60439, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Pfenninger
authors.forename: Shiu-Yung
authors.initials: SY
authors.lastname: Chan
authors.forename: Michael
authors.initials: M
authors.lastname: Karas
authors.forename: Berndt
authors.initials: B
authors.lastname: Finke
authors.forename: Bernd
authors.initials: B
authors.lastname: Stahl
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
keywords: FT-ICR MS; MALDI; carbohydrates; fucosylation; mixture analysis; sugars
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