title: Differential effects of organic and inorganic selenium compounds on adenosine deaminase activity and scavenger capacity in cerebral cortex slices of young rats.
abstract: Selenium (Se) has anti-inflammatory and antioxidant properties and is necessary for the development and normal function of the central nervous system. This study was aimed to compare the in vitro effects of 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one (C21H2HOSe; organoselenium) and sodium selenate (inorganic Se) on adenosine deaminase (ADA) activity, cell viability, lipid peroxidation, scavenger of nitric oxide (NO) and nonprotein thiols (NP-SH) content in the cerebral cortex slices of the young rats. A decrease in ADA activity was observed when the slices were exposed to organoselenium at the concentrations of 1, 10 and 30 µM. The same compound showed higher scavenger capacity of NO than the inorganic compound. Inorganic Se was able to protect against sodium nitroprusside-induced oxidative damage and increased the NP-SH content. Both the compounds displayed distinctive antioxidant capacities and were not cytotoxic for the cerebral cortex slices in the conditions tested. These findings are likely to be related to immunomodulatory and antioxidant properties of this compound.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Health Science Center, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: P E R
authors.initials: PE
authors.lastname: Bitencourt
authors.forename: L P
authors.initials: LP
authors.lastname: Bellé
authors.forename: G
authors.initials: G
authors.lastname: Bonfanti
authors.forename: L O
authors.initials: LO
authors.lastname: Cargnelutti
authors.forename: K S
authors.initials: KS
authors.lastname: de Bona
authors.forename: P S
authors.initials: PS
authors.lastname: Silva
authors.forename: F H
authors.initials: FH
authors.lastname: Abdalla
authors.forename: R A
authors.initials: RA
authors.lastname: Zanette
authors.forename: R B
authors.initials: RB
authors.lastname: Guerra
authors.forename: C
authors.initials: C
authors.lastname: Funchal
authors.forename: M B
authors.initials: MB
authors.lastname: Moretto
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D064586:Selenic Acid / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: C568342:3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one; D016166:Free Radical Scavengers; D016566:Organoselenium Compounds; D009569:Nitric Oxide; D000243:Adenosine Deaminase; D064586:Selenic Acid
keywords: Selenium; adenosine; cerebral cortex slices; lipid damage; scavenger