title: Baculovirus expression and diagnostic utility of the glycoprotein E of bovine herpesvirus-1.1 Egyptian strain "Abu-Hammad".
abstract: A recombinant baculovirus construct expressing glycoprotein E (gE) of the Egyptian BoHV-1.1 Abu-Hammad strain (rBac/gE-AbuH) was generated and characterized. The recombinant gE (rgE) secreting protein in culture medium of infected insect cells was used as a coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA) to test its utility for detection of antibody against gE of BoHV-1. Indirect gE-ELISA was compared to standard virus neutralization test and commercial blocking gE-ELISA for detection of anti-gE antibody in a panel of bovine sera. Antibody titers estimated by both ELISAs were closely correlated with those determined by virus neutralization test. In conclusion, the developed indirect gE-ELISA was a reliable candidate for inexpensive detection of anti-gE antibody in control and experimental bovine sera with high specificity and sensitivity. Moreover, it emphasized the diagnostic utility of gE based ELISAs to distinguish cattle infected with BoHV-1 from those vaccinated with the gE negative mutants.
authors.affiliation: Veterinary Serum and Vaccine Res. Instit., El-Sekka El-Beda St., P.O. Box 131, P.C. 11381 Abbassia, Cairo, Egypt. aaelfouly@yahoo.com
authors.forename: Alaa A
authors.initials: AA
authors.lastname: El-Kholy
authors.forename: Eman R
authors.initials: ER
authors.lastname: Abdou
authors.forename: Douaa I
authors.initials: DI
authors.lastname: Rady
authors.forename: Mai M
authors.initials: MM
authors.lastname: Elseafy
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002460:Cell Line; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005822:Genetic Vectors; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007313:Insecta; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification; D014773:Virology / Q000379:methods
chemical_list: D000914:Antibodies, Viral; D003470:Culture Media; D011994:Recombinant Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins