title: Evaluation of sesamol and buspirone in stress induced anxiety in mice.
abstract: OBJECTIVE
  The present study was designed to elucidate the effects of sesamol, buspirone and their combination in immobilization stress induced behavioral and biochemical alterations in mice.
  
  
  METHODS
  Male Laca mice (divided into 10 groups with 6 animals each) were pre-treated with sesamol (5 and 10 mg/kg; p.o.), buspirone (5 and 10 mg/kg; p.o.) and combination of sesamol (5 and 10 mg/kg; p.o.) with buspirone (5 mg/kg; p.o.) for consecutive five days. On the 6(th) day, animals were immobilized for 6 h and various behavioral tests such as body weight, locomotor activity, mirror chamber test and elevated plus maze were carried out. Biochemical estimations such as lipid peroxidation and nitrite concentration, glutathione and catalase levels were done. Data was analyzed using One way ANOVA followed by Tukey's test (P < 0.05) was considered statistical significant.
  
  
  RESULTS
  Immobilization stress significantly (P < 0.05) impaired body weight, locomotor activity, induced anxiety like behavioral and oxidative damage as compared to naοve animal. Pretreatment with sesamol (5 and 10 mg/kg; p.o.) and buspirone (5 and 10 mg/ kg; p.o.) significantly (P < 0.05) improved body weight, locomotor activity, and anxiety like behavior in mirror chamber as well as plus maze performance tasks and anti-oxidant like effect as evidenced by reduced lipid peroxidation, nitrite concentration and restoration of reduced glutathione and catalase activity as compared to control animals. Further, co- administration of sesamol (5 and 10 mg/kg) with buspirone (5 mg/kg) significantly (P < .05) potentiated the anti anxiety effects as compared to their effects alone.
  
  
  CONCLUSIONS
  The present study suggests that combination of sesamol and buspirone potentiated the antianxiety effects against anxiety induced by immobilization stress and oxidative damage in mice.
authors.affiliation: Department of Pharmacology, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh, Punjab, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Gurleen
authors.initials: G
authors.lastname: Kaur
authors.forename: Harikesh
authors.initials: H
authors.lastname: Kalonia
authors.forename: Puneet
authors.initials: P
authors.lastname: Rinwa
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001008:Anxiety Disorders / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D001522:Behavior, Animal / Q000187:drug effects; D052117:Benzodioxoles / Q000494:pharmacology*; D002065:Buspirone / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004359:Drug Therapy, Combination; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009573:Nitrites / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D014151:Anti-Anxiety Agents; D000975:Antioxidants; D052117:Benzodioxoles; D009573:Nitrites; D010636:Phenols; C025583:sesamol; D002374:Catalase; D005978:Glutathione; D002065:Buspirone
keywords: Anxiety; buspirone; immobilization; oxidative stress; sesamol
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