title: Antimutagenic and genoprotective effects of Saraca asoca bark extract.
abstract: OBJECTIVE
  Traditionally herbal formulations have been used effectively for the management of disorders that are now being accepted worldwide. Saraca asoca bark extract (SAE) finds use in traditional herbal medicine. In the present study, SAE were studied for their antioxidant, antimutagenic, and antigenotoxic properties.
  
  
  METHODS
  SAE were evaluated for antimutagenic property in Salmonella strains (TA97a, TA98, TA100, and TA102), in the presence and absence of metabolic activation (S9). The SAE was also studied for antigenotoxic property against cyclophosphamide (CP) in Swiss albino male mice in vivo. The extract was analyzed using high-performance liquid chromatography (HPLC).
  
  
  RESULTS
  The study reveals antimutagenic property of the bark extract in Salmonella strains in the presence and absence of metabolic activation (S9). The study reports antigenotoxic property of the bark extract against CP in vivo. Thiobarbituric acid reactive species assay on the bark extract revealed antioxidant property. HPLC revealed the presence of two peaks corresponding to gallic acid and (-)-epicatechin, respectively.
  
  
  CONCLUSIONS
  The study clearly reveals the antimutagenic and antigenotoxic properties of SAE.
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, University of Calcutta, Kolkata, India.
authors.forename: Debarati
authors.initials: D
authors.lastname: Nag
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, University of Calcutta, Kolkata, India anitamukherjee28@gmail.com gmanosij@gmail.com.
authors.forename: Manosij
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, University of Calcutta, Kolkata, India anitamukherjee28@gmail.com gmanosij@gmail.com.
authors.forename: Anita
authors.initials: A
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002392:Catechin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003520:Cyclophosphamide / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D007887:Fabaceae; D005707:Gallic Acid / Q000737:chemistry; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D005707:Gallic Acid; D003520:Cyclophosphamide; D002392:Catechin
keywords: Antigenotoxic; Saraca asoca; anitmutagenic; antioxidant; comet