title: Characterization and expression analysis of dirigent family genes related to stresses in Brassica.
abstract: The dirigent (DIR) genes are playing a vital role in enhancing stress resistance in different crop plants. In this study, we collected 29 DIR like genes, two from a Brassica rapa cv. Osome full length cDNA library and 27 from the B. rapa database designated as B. rapa Dirigent (BrDIR) like genes. Sequence analysis and a comparison study of these genes confirmed that seven were dirigent and the remaining 22 were dirigent like genes. Expression analysis revealed an organ specific expression of these genes. BrDIR2 showed differential responses after Fusarium oxysporum f.sp. conglutinans infection in cabbage. Four Brassica oleracea dirigent like genes highly homologous to BrDIR2 also showed similar responses in cabbage plants infected with this fungus. Moreover, several BrDIR like genes showed significant responses after water, ABA and cold stress treatments in Chinese cabbage. Under water stress, most responsive genes showed the highest expression at 24 h, at which time the acid soluble lignin content of samples under the same stress condition were also highest, indicating a possible relationship between BrDIR like genes and lignin content. Taken together, our results indicate a protective role of BrDIR genes against biotic and abiotic stresses in Brassica.
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, Republic of Korea.
authors.forename: Senthil Kumar
authors.initials: SK
authors.lastname: Thamil Arasan
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, Republic of Korea.
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Park
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, Republic of Korea.
authors.forename: Nasar Uddin
authors.initials: NU
authors.lastname: Ahmed
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, Republic of Korea.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Jung
authors.affiliation: Department of Biology, Chungnam National University, 96 Daehangno, Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Yoonkang
authors.initials: Y
authors.lastname: Hur
authors.affiliation: Department of Horticulture, Hankyong National University, 327 Chungangno, Anseong, Kyonggi 456-749, Republic of Korea.
authors.forename: Kwon-Kyoo
authors.initials: KK
authors.lastname: Kang
authors.affiliation: Department of Horticulture, Chungnam National University, 96 Daehangno, Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Yong-Pyo
authors.initials: YP
authors.lastname: Lim
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, Republic of Korea. Electronic address: nis@sunchon.ac.kr.
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
mesh_terms: D001937:Brassica / Q000187:drug effects / Q000378:metabolism*; D003080:Cold Temperature; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride
keywords: Biotic and abiotic stress; Brassica; Dirigent family genes; Expression analysis