title: Oxidative stress and inflammation in lean and obese subjects with polycystic ovary syndrome.
abstract: OBJECTIVE
  To determine whether polycystic ovary syndrome (PCOS) independently influences oxidative stress and inflammation or if the culprit is the comorbidities of obesity and/or insulin resistance common to this condition.
  
  
  METHODS
  Thirty women with PCOS were matched for age, body mass index and insulin resistance with 30 control subjects. Oxidative stress was examined by measuring the total oxidant status (TOS) and total antioxidant capacity (TAC) by spectrophotometric assay. The inflammatory biomarkers, C-reactive protein, plasminogen activator inhibitor-1, myeloperoxidase, neopterin, and serum amyloid A were measured by ELISA methodologies.
  
  
  RESULTS
  Oxidative status was increased in the PCOS subjects relative to their weight-matched controls (TOS: obese PCOS patients vs. obese controls, 42.42 +/- 4.49 vs. 32.57 +/- 1.97, p<0.05; lean PCOS patients vs. lean controls, 33.69 +/- 1.59 vs. 28.69 +/- 1.18 micromol H2O2 Equiv/L, p < 0.05). Furthermore, antioxidant capacity was lower in the lean PCOS group relative to their weight-matched controls (TAC: lean PCOS patients vs. lean controls, 1.10 +/- 0.09 vs. 1.49 +/- 0.03 nmol Trolox Equiv/L, p < 0.05).
  
  
  CONCLUSIONS
  These results suggest that PCOS independently influenced oxidative stress. Overall, the presence of PCOS may increase cardiovascular risk.
authors.affiliation: Nutrition and Metabolism Group, Centre for Public Health, Queen's University, Belfast, and the Department of Diabetes and Endocrinology, Adelaide and Meath Hospital, Dublin, U.K.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Blair
authors.forename: Tommy
authors.initials: T
authors.lastname: Kyaw-Tun
authors.forename: Ian S
authors.initials: IS
authors.lastname: Young
authors.forename: Niamh A
authors.initials: NA
authors.lastname: Phelan
authors.forename: James
authors.initials: J
authors.lastname: Gibney
authors.forename: Jane
authors.initials: J
authors.lastname: McEneny
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000975:Antioxidants; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000150:complications*; D019798:Neopterin / Q000097:blood; D009765:Obesity / Q000150:complications*; D018384:Oxidative Stress; D009195:Peroxidase / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D011085:Polycystic Ovary Syndrome / Q000150:complications*; D000685:Serum Amyloid A Protein / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D000975:Antioxidants; D017395:Plasminogen Activator Inhibitor 1; D000685:Serum Amyloid A Protein; D019798:Neopterin; D002097:C-Reactive Protein; D009195:Peroxidase