title: Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells.
abstract: Human mitochondrial ribosomal protein MRPS18-2 (S18-2) is encoded by a cellular gene that is located on the human chromosome 6p21.3. We discovered that overexpression of the S18-2 protein led to immortalization and de-differentiation of primary rat embryonic fibroblasts. Cells showed anchorage-independent growth pattern. Moreover, pathways characteristic for rapidly proliferating cells were upregulated then. It is possible that the S18-2 overexpression induced disturbance in cell cycle regulation. We found that overexpression of S18-2 protein in human cancer cell lines led to an appearance of multinucleated cells in the selected clones.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, 17177, Sweden.
authors.forename: Z
authors.initials: Z
authors.lastname: Shevchuk
authors.forename: M Y
authors.initials: MY
authors.lastname: Yurchenko
authors.forename: S D
authors.initials: SD
authors.lastname: Darekar
authors.forename: I
authors.initials: I
authors.lastname: Holodnuka-Kholodnyuk
authors.forename: V I
authors.initials: VI
authors.lastname: Kashuba
authors.forename: E V
authors.initials: EV
authors.lastname: Kashuba
keywords: Mitochondrial ribosomal protein S18-2 (MRPS18-2); RB binding protein; cancer cell line; cell cycle; multinucleated cells
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