title: Neuropeptide Y in black seabream Acanthopagrus schlegelii: identification, distribution and mRNA expression responses to ghrelin.
abstract: The coding region of neuropeptide Y (NPY) complementary (c)DNA was cloned from the hypothalamus RNA of black seabream Acanthopagrus schlegelii, including 297 bp coding for prepro-NPY of 98 amino acids. Real-time reverse transcriptase-polymerase chain reaction was performed to determine A. schlegelii npy gene expression; NPY messenger RNA (mRNA) was expressed highly in the brain and stomach. Treatment with acylated ghrelin significantly up-regulated NPY mRNA level in the hypothalamus, suggesting that NPY may be involved in regulating food intake of A. schlegelii.
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, 55 Zhongshan West Road, Guangzhou, 510631, PR China.
authors.forename: X L
authors.initials: XL
authors.lastname: Ma
authors.forename: L W
authors.initials: LW
authors.lastname: Zheng
authors.forename: L T
authors.initials: LT
authors.lastname: Mao
authors.forename: L B
authors.initials: LB
authors.lastname: Zhou
authors.forename: A L
authors.initials: AL
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D054439:Ghrelin / Q000494:pharmacology / Q000502:physiology*; D007031:Hypothalamus / Q000378:metabolism; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D021541:Sea Bream / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D054439:Ghrelin; D009478:Neuropeptide Y; D012333:RNA, Messenger