title: Oil-bioremediation potential of two hydrocarbonoclastic, diazotrophic Marinobacter strains from hypersaline areas along the Arabian Gulf coasts.
abstract: Two halophilic, hydrocarbonoclastics bacteria, Marinobacter sedimentarum and M. flavimaris, with diazotrophic potential occured in hypersaline waters and soils in southern and northern coasts of Kuwait. Their numbers were in the magnitude of 10(3) colony forming units g(-1). The ambient salinity in the hypersaline environments was between 3.2 and 3.5 M NaCl. The partial 16S rRNA gene sequences of the two strains showed, respectively, 99 and 100% similarities to the sequences in the GenBank. The two strains failed to grow in the absence of NaCl, exhibited best growth and hydrocarbon biodegradation in the presence of 1 to 1.5 M NaCl, and still grew and maintained their hydrocarbonoclastic activity at salinities up to 5 M NaCl. Both species utilized Tween 80, a wide range of individual aliphatic hydrocarbons (C9-C40) and the aromatics benzene, biphenyl, phenanthrene, anthracene and naphthalene as sole sources of carbon and energy. Experimental evidence was provided for their nitrogen-fixation potential. The two halophilic Marinobacter strains successfully mineralized crude oil in nutrient media as well as in hypersaline soil and water microcosms without the use of any nitrogen fertilizers.
authors.affiliation: Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University, PO Box 5969, 13060 Safat, Kuwait.
authors.forename: D M
authors.initials: DM
authors.lastname: Al-Mailem
authors.forename: M
authors.initials: M
authors.lastname: Eliyas
authors.forename: S S
authors.initials: SS
authors.lastname: Radwan
mesh_terms: D001673:Biodegradation, Environmental; D049109:Cell Proliferation; D017753:Ecosystem; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D007730:Kuwait; D053520:Marinobacter / Q000378:metabolism*; D009586:Nitrogen Fixation; D010578:Petroleum / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D012623:Seawater / Q000382:microbiology; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D006841:Hydrocarbons, Aromatic; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
references.citation: Appl Environ Microbiol. 1996 Apr;62(4):1405-15
references.pmid: 8919802
references.citation: Int J Syst Bacteriol. 1992 Oct;42(4):568-76
references.pmid: 1382536
references.citation: Appl Environ Microbiol. 2011 Nov;77(22):7962-74
references.pmid: 21948834
references.citation: Extremophiles. 2010 Mar;14(2):225-31
references.pmid: 20157750
references.citation: Appl Microbiol Biotechnol. 2012 Aug;95(3):789-98
references.pmid: 22086071
references.citation: Int J Syst Evol Microbiol. 2007 Feb;57(Pt 2):250-254
references.pmid: 17267959
references.citation: Antonie Van Leeuwenhoek. 2013 Mar;103(3):485-91
references.pmid: 23117603
references.citation: Extremophiles. 2010 May;14(3):321-8
references.pmid: 20364355
references.citation: Appl Environ Microbiol. 1993 Mar;59(3):695-700
references.pmid: 7683183
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1389-1395
references.pmid: 13130023
references.citation: Adv Microb Physiol. 1971;5:1-43
references.pmid: 4950258
references.citation: Int J Syst Evol Microbiol. 2003 Mar;53(Pt 2):377-380
references.pmid: 12710601
references.citation: Appl Environ Microbiol. 2006 May;72(5):3531-42
references.pmid: 16672500
references.citation: Extremophiles. 2012 Sep;16(5):751-8
references.pmid: 22868892
references.citation: Appl Microbiol Biotechnol. 2011 Aug;91(3):823-34
references.pmid: 21562979
references.citation: Int J Syst Bacteriol. 1999 Apr;49 Pt 2:367-75
references.pmid: 10319457
references.citation: Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1453-1456
references.pmid: 16014465
references.citation: Curr Opin Biotechnol. 2007 Jun;18(3):257-66
references.pmid: 17493798
references.citation: Microbiol Rev. 1990 Sep;54(3):305-15
references.pmid: 2215423
references.citation: Chemosphere. 2011 Sep;84(11):1671-6
references.pmid: 21621813
references.citation: Environ Pollut. 1990;65(1):1-17
references.pmid: 15092275
references.citation: Appl Environ Microbiol. 2002 Dec;68(12):6246-55
references.pmid: 12450849
references.citation: Arch Microbiol. 2012 Aug;194(8):689-705
references.pmid: 22398928
references.citation: Mar Pollut Bull. 2012 Jan;64(1):7-12
references.pmid: 22130193
references.citation: Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1349-1351
references.pmid: 15879281
references.citation: Int J Syst Evol Microbiol. 2006 Mar;56(Pt 3):523-527
references.pmid: 16514021
references.citation: Can J Microbiol. 2003 Nov;49(11):713-21
references.pmid: 14735221