title: Deep cerebellar nuclei play an important role in two-tone discrimination on delay eyeblink conditioning in C57BL/6 mice.
abstract: Previous studies have shown that deep cerebellar nuclei (DCN)-lesioned mice develop conditioned responses (CR) on delay eyeblink conditioning when a salient tone conditioned stimulus (CS) is used, which suggests that the cerebellum potentially plays a role in more complicated cognitive functions. In the present study, we examined the role of DCN in tone frequency discrimination in the delay eyeblink-conditioning paradigm. In the first experiment, DCN-lesioned and sham-operated mice were subjected to standard simple eyeblink conditioning under low-frequency tone CS (LCS: 1 kHz, 80 dB) or high-frequency tone CS (HCS: 10 kHz, 70 dB) conditions. DCN-lesioned mice developed CR in both CS conditions as well as sham-operated mice. In the second experiment, DCN-lesioned and sham-operated mice were subjected to two-tone discrimination tasks, with LCS+ (or HCS+) paired with unconditioned stimulus (US), and HCS- (or LCS-) without US. CR% in sham-operated mice increased in LCS+ (or HCS+) trials, regardless of tone frequency of CS, but not in HCS- (or LCS-) trials. The results indicate that sham-operated mice can discriminate between LCS+ and HCS- (or HCS+ and LCS-). In contrast, DCN-lesioned mice showed high CR% in not only LCS+ (or HCS+) trials but also HCS- (or LCS-) trials. The results indicate that DCN lesions impair the discrimination between tone frequency in eyeblink conditioning. Our results suggest that the cerebellum plays a pivotal role in the discrimination of tone frequency.
authors.affiliation: Laboratory for Behavioral Neuroendocrinology, Graduate School of Comprehensive Human Science, University of Tsukuba, Tsukuba, Ibaraki, Japan. sakamoto-t@tachibana-u.ac.jp
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Shogo
authors.initials: S
authors.lastname: Endo
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001767:Blinking; D002529:Cerebellar Nuclei / Q000502:physiology*; D002531:Cerebellum / Q000378:metabolism* / Q000502:physiology; D003071:Cognition; D003214:Conditioning, Classical / Q000502:physiology*; D003215:Conditioning, Eyelid / Q000502:physiology*; D004192:Discrimination, Psychological; D004566:Electrodes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
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