title: Enhancement of in vivo antioxidant ability in the brain of rats fed tannin.
abstract: The effect of the oral administration of mimosa tannin (MMT) on the rat intra-hippocampal antioxidant ability was examined. Wistar rats at the age of 6 weeks were reared for 8 weeks with the rodent diet (RD) consisting of 0.1 g/kg of MMT (RD-MMT). The antioxidant ability of rat brain was evaluated from the decay of a brain-blood-barrier permeable stable nitroxide, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (PCAM) measured by the microdialysis-electron spin resonance system under a freely moving state. The decay rate of PCAM in the brain of rats fed RD-MMT was significantly larger than that of rats fed control rodent diet, which indicates the increase of the antioxidant ability in the brain of rats fed RD-MMT. In vitro study showed that MMT did not reduce PCAM directly but enhanced the reduction of PCAM by ascorbic acid. These results indicate that MMT is a potent antioxidant in vitro and in vivo.
authors.affiliation: Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan. akanaka@med.miyazaki-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Nakajima
authors.forename: Yuto
authors.initials: Y
authors.lastname: Ueda
authors.forename: Emiko
authors.initials: E
authors.lastname: Matsuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sameshima
authors.forename: Tsuyomu
authors.initials: T
authors.lastname: Ikenoue
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000737:chemistry; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000493:pharmacokinetics; D004578:Electron Spin Resonance Spectroscopy; D017551:Microdialysis; D011759:Pyrrolidines / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013634:Tannins / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; C109806:MCPROXYL; D011759:Pyrrolidines; D013634:Tannins; D001205:Ascorbic Acid
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