title: Bulk segregant analysis of an induced floral mutant identifies a MIXTA-like R2R3 MYB controlling nectar guide formation in Mimulus lewisii.
abstract: The genetic and developmental basis of many ecologically important floral traits (e.g., carotenoid pigmentation, corolla tube structure, nectar volume, pistil and stamen length) remains poorly understood. Here we analyze a chemically induced floral mutant of Mimulus lewisii through bulk segregant analysis and transgenic experiments and identify a MIXTA-like R2R3 MYB gene that controls nectar guide formation in M. lewisii flowers, which involves epidermal cell development and carotenoid pigmentation.
authors.affiliation: Department of Biology, University of Washington, Seattle, WA 98195, USA. yuan.colreeze@gmail.com
authors.forename: Yao-Wu
authors.initials: YW
authors.lastname: Yuan
authors.forename: Janelle M
authors.initials: JM
authors.lastname: Sagawa
authors.forename: Verónica S
authors.initials: VS
authors.lastname: Di Stilio
authors.forename: H D
authors.initials: HD
authors.lastname: Bradshaw
mesh_terms: D003433:Crosses, Genetic; D035264:Flowers / Q000033:anatomy & histology / Q000235:genetics*; D032241:Mimulus / Q000235:genetics*; D009154:Mutation; D010858:Pigmentation / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: MIXTA-like MYB; Mimulus; carotenoids; nectar guides; trichome
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