title: Novel genes detected by transcriptional profiling from whole-blood cells in patients with early onset of acute coronary syndrome.
abstract: BACKGROUND
  Genome-wide expression analysis using microarrays has been used as a research strategy to discovery new biomarkers and candidate genes for a number of diseases. We aim to find new biomarkers for the prediction of acute coronary syndrome (ACS) with a differentially expressed mRNA profiling approach using whole genomic expression analysis in a peripheral blood cell model from patients with early ACS.
  
  
  RESULTS
  This study was carried out in two phases. On phase 1 a restricted clinical criteria (ACS-Ph1, n=9 and CG-Ph1, n=6) was used in order to select potential mRNA biomarkers candidates. A subsequent phase 2 study was performed using selected phase 1 markers analyzed by RT-qPCR using a larger and independent casuistic (ACS-Ph2, n=74 and CG-Ph2, n=41). A total of 549 genes were found to be differentially expressed in the first 48 h after the ACS-Ph1. Technical and biological validation further confirmed that ALOX15, AREG, BCL2A1, BCL2L1, CA1, COX7B, ECHDC3, IL18R1, IRS2, KCNE1, MMP9, MYL4 and TREML4, are differentially expressed in both phases of this study.
  
  
  CONCLUSIONS
  Transcriptomic analysis by microarray technology demonstrated differential expression during a 48 h time course suggesting a potential use of some of these genes as biomarkers for very early stages of ACS, as well as for monitoring early cardiac ischemic recovery.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil. viviansilbiger@hotmail.com
authors.forename: Vivian N
authors.initials: VN
authors.lastname: Silbiger
authors.forename: André D
authors.initials: AD
authors.lastname: Luchessi
authors.forename: Rosário D C
authors.initials: RD
authors.lastname: Hirata
authors.forename: Lídio G
authors.initials: LG
authors.lastname: Lima-Neto
authors.forename: Débora
authors.initials: D
authors.lastname: Cavichioli
authors.forename: Angel
authors.initials: A
authors.lastname: Carracedo
authors.forename: Maria
authors.initials: M
authors.lastname: Brión
authors.forename: Joaquín
authors.initials: J
authors.lastname: Dopazo
authors.forename: Francisco
authors.initials: F
authors.lastname: García-García
authors.forename: Elizabete S
authors.initials: ES
authors.lastname: dos Santos
authors.forename: Rui F
authors.initials: RF
authors.lastname: Ramos
authors.forename: Marcelo F
authors.initials: MF
authors.lastname: Sampaio
authors.forename: Dikran
authors.initials: D
authors.lastname: Armaganijan
authors.forename: Amanda G M R
authors.initials: AG
authors.lastname: Sousa
authors.forename: Mario H
authors.initials: MH
authors.lastname: Hirata
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001773:Blood Cells / Q000737:chemistry / Q000378:metabolism*; D042241:Early Diagnosis; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D059467:Transcriptome
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; D012333:RNA, Messenger