title: Acetylcholinesterase and butyrylcholinesterase activities in obese Beagle dogs before and after weight loss.
abstract: BACKGROUND
  Obesity is the most common nutritional disorder in today's dog population and the major risk factor for a number of related diseases. However, the exact pathogenesis of obesity-related complications is not always clear. In people, butyrylcholinesterase (BChE) is suspected to be involved in lipoprotein metabolism and has also been associated with the pathogenesis of inflammatory disease, one of the potential complications related to obesity.
  
  
  OBJECTIVE
  The aim of the study was to evaluate the effect of experimentally induced weight loss on BChE and acetylcholinesterase (AChE) in obese dogs to elucidate the possible relationship between these 2 enzymes and obesity.
  
  
  METHODS
  Six obese intact female Beagle dogs were allocated to a weight loss program for 3 months. BChE was measured in serum samples using butyrylcholine as substrate, whereas AChE was measured in whole blood after inhibition of BChE with ethopropazine and using acetylcholine as a substrate.
  
  
  RESULTS
  After rapid weight loss serum BChE activities were statistically significantly lower (P < .05), whereas AChE activities were higher (P < .01). There was a positive correlation between serum BChE activity and concentrations of total cholesterol (TCHOL, P < .001), low-density lipoprotein-cholesterol (LDL-C, P < .001), and triglycerides (P < .05). A negative correlation was detected between serum BChE and AChE activities (P < .0001), and between AChE activity and serum levels of TCHOL (P < .01), LDL-C (P < .01) and high-density lipoprotein-cholesterol (P < .05).
  
  
  CONCLUSIONS
  Short-term weight loss in obese intact female Beagle dogs resulted in opposite effects in 2 cholinesterase isoenzyme activities, namely lower BChE and higher AChE activities.
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia, Spain.
authors.forename: Asta
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Ceron
authors.forename: Fernando
authors.initials: F
authors.lastname: Tecles
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000110:Acetylcholinesterase / Q000097:blood / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000097:blood / Q000187:drug effects / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D005260:Female; D009765:Obesity / Q000201:enzymology / Q000662:veterinary*; D010640:Phenothiazines / Q000494:pharmacology; D015431:Weight Loss / Q000502:physiology*
chemical_list: D000924:Anticholesteremic Agents; D002800:Cholinesterase Inhibitors; D010640:Phenothiazines; C084820:profenamine; D002784:Cholesterol; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000109:Acetylcholine