title: Evaluation of the antifungal activity of four solutions used as a final rinse in vitro.
abstract: The aim of the study was to compare the antifungal activity of 1.3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), MTAD and Tetraclean as a final rinse against Candida albicans in a human tooth model in vitro. Ninety extracted human maxillary central and lateral incisor teeth were randomly divided into four groups each with 20 teeth, a positive and a negative control each with five teeth. After preparing the root canals, teeth were inoculated with Candida albicans (ATCC 10261) and incubated for 72 h. Teeth were divided into four experimental groups according to the irrigation solution as follows: NaOCl, CHX, MTAD and Tetraclean. After culturing aliquots from the experimental teeth on Sabouraud 4% dextrose agar, colony-forming units were counted. The results showed that 1.3% NaOCl and 2% CHX were equally effective and significantly superior to MTAD and Tetraclean (P < 0.05). Furthermore, antifungal efficacy of Tetraclean was significantly superior to MTAD (P < 0.05).
authors.affiliation: Department of Endodontics, Hamedan University of Medical Sciences, Hamedan, Iran. mohammadi_zahed@yahoo.com
authors.forename: Zahed
authors.initials: Z
authors.lastname: Mohammadi
authors.forename: Luciano
authors.initials: L
authors.lastname: Giardino
authors.forename: Flavio
authors.initials: F
authors.lastname: Palazzi
mesh_terms: D000935:Antifungal Agents / Q000627:therapeutic use*; D002176:Candida albicans / Q000187:drug effects; D002593:Cetrimonium Compounds / Q000627:therapeutic use; D002710:Chlorhexidine / Q000627:therapeutic use; D019343:Citric Acid / Q000627:therapeutic use; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004318:Doxycycline / Q000627:therapeutic use; D006801:Humans; D007180:Incisor / Q000382:microbiology; D011126:Polypropylenes / Q000627:therapeutic use; D011136:Polysorbates / Q000627:therapeutic use; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000627:therapeutic use
chemical_list: D000935:Antifungal Agents; D002593:Cetrimonium Compounds; C475043:MTAD (intracanal irrigant); D011126:Polypropylenes; D011136:Polysorbates; D012388:Root Canal Irrigants; C555783:Tetraclean; D019343:Citric Acid; D012973:Sodium Hypochlorite; D004318:Doxycycline; D002710:Chlorhexidine