title: MicroRNA-150: a novel marker of left ventricular remodeling after acute myocardial infarction.
abstract: BACKGROUND
  Left ventricular (LV) remodeling after acute myocardial infarction is associated with adverse prognosis. MicroRNAs (miRNAs) regulate the expression of several genes involved in LV remodeling. Our aim was to identify miRNAs associated with LV remodeling after acute myocardial infarction.
  
  
  RESULTS
  We studied 90 patients after first ST-segment-elevation acute myocardial infarction. A derivation cohort consisted of 60 patients characterized by echocardiography predischarge and at 6-month follow-up. Thirty patients characterized by magnetic resonance imaging predischarge and at 4-month follow-up were the validation cohort. Remodeling was defined as an increase in LV end-diastolic volume (ΔEDV>0) between discharge and follow-up. Circulating miRNAs were measured by microarrays and polymerase chain reaction. Using a systems-based approach, we identified several miRNAs potentially involved in LV remodeling. In the derivation cohort, one of these miRNAs, miR-150, was downregulated in patients with remodeling (ΔEDV>0) compared with patients without remodeling (ΔEDV≤0). In the validation cohort, patients with remodeling had 2-fold lower levels of miR-150 than those without (P=0.03). miR-150 outperformed N-terminal pro-brain natriuretic peptide to predict remodeling (area under the receiver-operating characteristic curve of 0.74 and 0.60, respectively). miR-150 reclassified 54% (95% confidence interval, 5-102; P=0.03) of patients misclassified by N-terminal pro-brain natriuretic peptide and 59% (95% confidence interval, 9-108; P=0.02) of patients misclassified by a multiparameter clinical model, including age, sex, and admission levels of troponin I, creatine kinase, and N-terminal pro-brain natriuretic peptide.
  
  
  CONCLUSIONS
  Low circulating levels of miR-150 are associated with LV remodeling after first ST-segment-elevation acute myocardial infarction. miR-150 has potential as a novel biomarker in this setting.
authors.affiliation: Laboratory of Cardiovascular Research, Centre de Recherche Public-Santé, Luxembourg. yvan.devaux@crp-sante.lu
authors.forename: Yvan
authors.initials: Y
authors.lastname: Devaux
authors.forename: Melanie
authors.initials: M
authors.lastname: Vausort
authors.forename: Gerry P
authors.initials: GP
authors.lastname: McCann
authors.forename: Jennifer
authors.initials: J
authors.lastname: Zangrando
authors.forename: Dominic
authors.initials: D
authors.lastname: Kelly
authors.forename: Naveed
authors.initials: N
authors.lastname: Razvi
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Leong L
authors.initials: LL
authors.lastname: Ng
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Wagner
authors.forename: Iain B
authors.initials: IB
authors.lastname: Squire
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000235:genetics / Q000503:physiopathology*; D020257:Ventricular Remodeling
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C533097:MIR150, human
keywords: biomarker; microRNA; myocardial infarction; remodeling