title: Anti-viral activity of galectin-1 from flounder Paralichthys olivaceus.
abstract: Galectins are a family of Ca(2+)-independent soluble lectins characterized by their affinity to β-galactosides. Mammalian galectins have been shown to play a defense role against certain bacteria, fungi and viruses. However, the immunological functions of galectins in fish is poorly characterized. Here we demonstrated that the expression of galectin-1 gene from the flounder Paralichthys olivaceus was decreased in the initial 8 h after challenge with poly I:C, then increased markedly from 24 h onwards, and the recombinant galectin-1 was able to neutralize the lymphocystis disease virus (LCDV), inhibiting the formation of cytopathic effects. In addition, the recombinant galectin had a potential anti-inflammatory activity against infection by LCDV, and was able to restrain the overexpression of the anti-viral protein gene mx against virus infection. These results indicate that flounder galectin-1 has an anti-viral activity, capable of reducing LCDV pathogenicity.
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, China.
authors.forename: Shousheng
authors.initials: S
authors.lastname: Liu
authors.forename: Guobin
authors.initials: G
authors.lastname: Hu
authors.forename: Chen
authors.initials: C
authors.lastname: Sun
authors.forename: Shicui
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D002460:Cell Line; D003001:Cloning, Molecular; D019610:Cytoprotection; D004266:DNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005432:Flounder; D037483:Galectin 1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007497:Iridoviridae / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D000998:Antiviral Agents; D018076:DNA, Complementary; D029941:Fish Proteins; D037483:Galectin 1; D011994:Recombinant Proteins