title: Chromosome analysis of nuclear power plant workers using fluorescence in situ hybridization and Giemsa assay.
abstract: The aim of this study was to evaluate the genotoxic effects of ionizing radiation in vivo in exposed Bulgarian nuclear power plant workers by using classical cytogenetic and molecular cytogenetic analyses of peripheral lymphocytes. Chromosome analysis using fluorescence in situ hybrydization (FISH) and Giemsa techniques was undertaken on 63 workers and 45 administrative staff controls from the Bulgarian Nuclear Power Plant. Using the Giemsa method, the frequencies of cells studied with chromosome aberrations, dicentrics plus rings and chromosome fragments in the radiation workers were significantly higher compared with the control group (P = 0.044, P = 0.014, and P = 0.033, respectively). A significant association between frequencies of dicentrics plus rings and accumulated doses was registered (P < 0.01). In the present study, a FISH cocktail of whole chromosome paints for chromosomes 1, 4 and 11 was used. A significant association between frequency of translocations and accumulated doses was also observed (P < 0.001). Within the control group, a correlation was found between age and the spontaneous frequency of translocations. No correlation was found between smoking status and frequency of translocations. When compared with the control group, workers with accumulated doses up to 100 mSv showed no increase in genome translocation frequency, whereas workers with accumulated doses from 101 to 200 mSv showed a statistically significant doubling of genome translocation frequency (P = 0.009). Thus, in cases of chronic exposure and for purposes of retrospective dosimetry, the genome frequency of translocations is a more useful marker for evaluation of genotoxic effects than dicentric frequency.
authors.affiliation: Department of Radiation Genetics, National Centre of Radiobiology and Radiation Protection, 3 Sv. Georgi Sofiiski Blvd, 1606 Sofia, Bulgaria.
authors.forename: Rositsa
authors.initials: R
authors.lastname: Hristova
authors.forename: Valeria
authors.initials: V
authors.lastname: Hadjidekova
authors.forename: Mira
authors.initials: M
authors.lastname: Grigorova
authors.forename: Teodora
authors.initials: T
authors.lastname: Nikolova
authors.forename: Minka
authors.initials: M
authors.lastname: Bulanova
authors.forename: Ljubomira
authors.initials: L
authors.lastname: Popova
authors.forename: Albena
authors.initials: A
authors.lastname: Staynova
authors.forename: Donka
authors.initials: D
authors.lastname: Benova
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000453:epidemiology; D001399:Azure Stains; D001681:Biological Assay / Q000379:methods / Q000706:statistics & numerical data*; D002031:Bulgaria / Q000453:epidemiology; D002869:Chromosome Aberrations / Q000528:radiation effects* / Q000706:statistics & numerical data*; D015897:Comorbidity; D020732:Cytogenetic Analysis / Q000706:statistics & numerical data; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000706:statistics & numerical data*; D008297:Male; D008875:Middle Aged; D055848:Nuclear Power Plants / Q000706:statistics & numerical data*; D016273:Occupational Exposure; D015995:Prevalence; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000453:epidemiology*; D015203:Reproducibility of Results; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012907:Smoking / Q000453:epidemiology; D055815:Young Adult
chemical_list: D001399:Azure Stains
keywords: FISH; chromosome aberrations; dose accumulation; nuclear power plant workers; occupational exposure
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