title: FlgM as a secretion moiety for the development of an inducible type III secretion system.
abstract: Regulation and assembly of the flagellar type III secretion system is one of the most investigated and best understood regulational cascades in molecular biology. Depending on the host organism, flagellar morphogenesis requires the interplay of more than 50 genes. Direct secretion of heterologous proteins to the supernatant is appealing due to protection against cellular proteases and simplified downstream processing. As Escherichia coli currently remains the predominant host organism used for recombinant prokaryotic protein expression, the generation of a strain that exhibits inducible flagellar secretion would be highly desirable for biotechnological applications. Here, we report the first engineered Escherichia coli mutant strain featuring flagellar morphogenesis upon addition of an external inducer. Using FlgM as a sensor for direct secretion in combination with this novel strain may represent a potent tool for significant improvements in future engineering of an inducible type III secretion for heterologous proteins.
authors.affiliation: Institute of Biochemistry, University of Innsbruck, Innsbruck, Austria. Thomash@caltech.edu
authors.forename: Thomas
authors.initials: T
authors.lastname: Heel
authors.forename: Georg F
authors.initials: GF
authors.lastname: Vogel
authors.forename: Andrea
authors.initials: A
authors.lastname: Lammirato
authors.forename: Rainer
authors.initials: R
authors.lastname: Schneider
authors.forename: Bernhard
authors.initials: B
authors.lastname: Auer
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D023061:Gene Order; D009154:Mutation; D009876:Operon; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; C071266:FlgM protein, Bacteria
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