title: MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells.
abstract: MicroRNAs regulate self renewal and differentiation of cancer stem cells. There, we sought to identify the expression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study. MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting. After miR-181b was overexpressed in U87 glioma stem cells by miR-181b lentiviral expression vector and/or treatment of temozolomide, secondary neurosphere assay, soft agar colony assay and MTT assay were performed. Compared with U87 cells, the expression of miR-181b was significantly decreased in U87 glioma stem cells. Overexpression of miR-181b decreased neurosphere formation by U87 glioma stem cells in vitro and suppressed colony formation in soft agar, and the cell growth inhibition rates increased in a time-dependent manner in U87 glioma stem cells infected with miR-181b lentivirus. Furthermore, miR-181b had a synergistic effect on temozolomide-induced inhibition of secondary neurosphere and soft agar colony, and on cell growth inhibition rates. MiR-181b functions as a tumor suppressor that suppresses proliferation and reduces chemoresistance to temozolomide in glioma stem cells.
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lu
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Wang
authors.forename: Lihua
authors.initials: L
authors.lastname: Sun
authors.forename: Chunfa
authors.initials: C
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Yongping
authors.initials: Y
authors.lastname: You
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fu
keywords: chemoresistance; glioma stem cells; miR-181b; proliferation
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