title: Cloning and expression analysis of Foxl2 during the reproductive cycle in Korean rockfish, Sebastes schlegeli.
abstract: Foxl2 is a member of the winged helix/forkhead family of transcription factors and is known to regulate ovarian aromatase, which plays a crucial role in ovarian differentiation. To address the role of Foxl2 in gonads and brain during gonadal development, we isolated the full-length cDNA of Foxl2 and analyzed its spatiotemporal expression patterns in the viviparous teleost Korean rockfish, Sebastes schlegeli. Tissue distribution pattern revealed that the Foxl2 was detected in the liver, fat, gill, brain, and ovary, but could hardly be found in the testis. Reverse transcriptase PCR suggested that Foxl2 in Korean rockfish may involve in ovary development in the study of expression level during gonads development. It also revealed that the stage of highest expression level for Foxl2 was almost much earlier than cyp19a1a and cyp19a1b during the gonadal development stage in gonads and brain except for cyp19a1a in brain. Furthermore, the expression pattern of Foxl2 as well as aromatases may imply the role of Foxl2 in the up-regulation of aromatases not only in the female fish but also in male.
authors.affiliation: Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.
authors.forename: Wei J
authors.initials: WJ
authors.lastname: Mu
authors.forename: Hai S
authors.initials: HS
authors.lastname: Wen
authors.forename: Ji F
authors.initials: JF
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D005260:Female; D005399:Fishes / Q000378:metabolism*; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000201:enzymology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D012098:Reproduction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; D001141:Aromatase
references.citation: Am J Hum Genet. 1983 Sep;35(5):1020-7
references.pmid: 6613996
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2006 Sep;145(1):47-53
references.pmid: 16782377
references.citation: Mol Reprod Dev. 2007 Oct;74(10):1239-46
references.pmid: 17342735
references.citation: Cell. 2009 Dec 11;139(6):1130-42
references.pmid: 20005806
references.citation: Biochem Biophys Res Commun. 2004 Jul 16;320(1):83-9
references.pmid: 15207705
references.citation: Gen Comp Endocrinol. 2008 May 15;157(1):75-85
references.pmid: 18452918
references.citation: J Med Genet. 2002 Dec;39(12):916-21
references.pmid: 12471206
references.citation: Am J Hum Genet. 2003 Feb;72(2):478-87
references.pmid: 12529855
references.citation: Fish Physiol Biochem. 2012 Jun;38(3):653-64
references.pmid: 21850400
references.citation: Gen Comp Endocrinol. 2011 Dec 1;174(3):259-68
references.pmid: 21906596
references.citation: Sex Dev. 2011;5(4):213-23
references.pmid: 21720151
references.citation: Fish Physiol Biochem. 2013 Apr;39(2):287-98
references.pmid: 22843313
references.citation: Mol Endocrinol. 2007 Mar;21(3):712-25
references.pmid: 17192407
references.citation: Mol Cell Endocrinol. 2003 Aug 29;206(1-2):93-111
references.pmid: 12943993
references.citation: Nat Genet. 2001 Dec;29(4):453-8
references.pmid: 11726932
references.citation: J Mol Endocrinol. 2009 May;42(5):437-48
references.pmid: 19293249
references.citation: Reproduction. 2010 Sep;140(3):477-87
references.pmid: 20584991
references.citation: Biol Reprod. 2005 Nov;73(5):959-66
references.pmid: 16014816
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2009 Sep;154(1):134-42
references.pmid: 19481171
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Genesis. 2009 May;47(5):289-99
references.pmid: 19298014
references.citation: Cytogenet Genome Res. 2003;101(3-4):199-205
references.pmid: 14684983
references.citation: Biochem Biophys Res Commun. 2007 Aug 10;359(4):935-40
references.pmid: 17574208
references.citation: Steroids. 2011 Dec 20;76(14):1597-608
references.pmid: 22005200
references.citation: Dev Biol. 2002 Oct 1;250(1):1-23
references.pmid: 12297093
references.citation: Dev Dyn. 2004 Dec;231(4):859-70
references.pmid: 15517586
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Nat Genet. 2001 Feb;27(2):159-66
references.pmid: 11175783
references.citation: Mamm Genome. 2000 Feb;11(2):151-8
references.pmid: 10656931
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2010 Feb;155(2):118-25
references.pmid: 19895900
references.citation: Gen Comp Endocrinol. 2008 Nov-Dec;159(2-3):170-7
references.pmid: 18805419
references.citation: Endocrinology. 2003 Jul;144(7):3237-43
references.pmid: 12810580
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712