title: Daphnane-type diterpenes with inhibitory activities against human cancer cell lines from Daphne genkwa.
abstract: Four new daphnane-type diterpenes, genkwadanes A-D (1-4), together with 19 known ones, were isolated from ethanol extract of the flower buds of Daphne genkwa. Their structures were determined on the basis of extensive spectroscopic data. Among them, daphnane-type diterpene with a 1,10-double bond (1) was isolated from this plant for the first time. The cytotoxicity of all compounds 1-23 against the 10 selected human cancer cell lines was assayed. A number of compounds exhibited significant activities against the 10 cancer cell lines (IC50<9.56 μM). and most interestingly, all the compounds revealed preferred cytotoxicities on the HT-1080 cell line and displayed much stronger inhibitory activities (IC50<29.94 μM) compared with positive control 5-fluorouracil (IC50=35.62 μM), particularly, compounds 9-11, 13, 16 and 19 exhibited the strongest cytotoxicity activities against the HT-1080 cell line (IC50<0.1 μM).
authors.affiliation: Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Lili
authors.initials: L
authors.lastname: Hong
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Li
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.forename: Mingyu
authors.initials: M
authors.lastname: Xia
authors.forename: Takshi
authors.initials: T
authors.lastname: Ikejima
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Shaojiang
authors.initials: S
authors.lastname: Song
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D032364:Daphne / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D035264:Flowers / Q000737:chemistry; D045325:HCT116 Cells; D018922:HL-60 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020014:K562 Cells; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes