title: A triad of highly divergent polymeric immunoglobulin receptor (PIGR) haplotypes with major effect on IgA concentration in bovine milk.
abstract: The aim of this study was to determine a genetic basis for IgA concentration in milk of Bos taurus. We used a Holstein-Friesian x Jersey F2 crossbred pedigree to undertake a genome-wide search for QTL influencing IgA concentration and yield in colostrum and milk. We identified a single genome-wide significant QTL on chromosome 16, maximising at 4.8 Mbp. The polymeric immunoglobulin receptor gene (PIGR) was within the confidence interval of the QTL. In addition, mRNA expression analysis revealed a liver PIGR expression QTL mapping to the same locus as the IgA quantitative trait locus. Sequencing and subsequent genotyping of the PIGR gene revealed three divergent haplotypes that explained the variance of both the IgA QTL and the PIGR expression QTL. Genetic selection based on these markers will facilitate the production of bovine herds producing milk with higher concentrations of IgA.
authors.affiliation: ViaLactia Biosciences, Auckland, New Zealand. sarah.berry@auckland.ac.nz
authors.forename: Sarah
authors.initials: S
authors.lastname: Berry
authors.forename: Wouter
authors.initials: W
authors.lastname: Coppieters
authors.forename: Stephen
authors.initials: S
authors.lastname: Davis
authors.forename: Alayna
authors.initials: A
authors.lastname: Burrett
authors.forename: Natalie
authors.initials: N
authors.lastname: Thomas
authors.forename: David
authors.initials: D
authors.lastname: Palmer
authors.forename: Van
authors.initials: V
authors.lastname: Kelly
authors.forename: Vladimir
authors.initials: V
authors.lastname: Obolonkin
authors.forename: Kathryn
authors.initials: K
authors.lastname: Sanders
authors.forename: Richard
authors.initials: R
authors.lastname: Spelman
authors.forename: Michel
authors.initials: M
authors.lastname: Georges
authors.forename: Klaus
authors.initials: K
authors.lastname: Lehnert
authors.forename: Russell
authors.initials: R
authors.lastname: Snell
mesh_terms: D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D003126:Colostrum / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D007070:Immunoglobulin A / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008892:Milk / Q000737:chemistry*; D010641:Phenotype; D040641:Quantitative Trait Loci; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics* / Q000378:metabolism; D012635:Secretory Component / Q000235:genetics / Q000378:metabolism
chemical_list: D007070:Immunoglobulin A; D019056:Receptors, Polymeric Immunoglobulin; D012635:Secretory Component
references.citation: J Dairy Sci. 2007 Oct;90(10):4846-55
references.pmid: 17881708
references.citation: J Dairy Sci. 1985 Sep;68(9):2309-14
references.pmid: 4067046
references.citation: J Anim Sci. 2009 Apr;87(13 Suppl):3-9
references.pmid: 18952725
references.citation: N Z Vet J. 2010 Feb;58(1):1-5
references.pmid: 20200568
references.citation: J Dairy Sci. 2010 Nov;93(11):5467-73
references.pmid: 20965362
references.citation: Can J Comp Med. 1972 Jul;36(3):234-42
references.pmid: 4261838
references.citation: J Dairy Sci. 1986 Jul;69(7):1799-805
references.pmid: 3745582
references.citation: Genome Res. 2002 Feb;12(2):222-31
references.pmid: 11827942
references.citation: Nat Genet. 2011 May;43(5):405-13
references.pmid: 21516082
references.citation: Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8796-800
references.pmid: 1924341
references.citation: Bioinformatics. 2009 Jul 15;25(14):1754-60
references.pmid: 19451168
references.citation: J Dairy Sci. 2007 Dec;90(12):5490-8
references.pmid: 18024740
references.citation: Genetics. 2003 Jan;163(1):253-66
references.pmid: 12586713
references.citation: Genetics. 1995 Nov;141(3):1189-97
references.pmid: 8582623
references.citation: J Mammary Gland Biol Neoplasia. 2007 Dec;12(4):237-47
references.pmid: 17992474
references.citation: Br J Nutr. 2000 Nov;84 Suppl 1:S75-80
references.pmid: 11242450
references.citation: J Mammary Gland Biol Neoplasia. 2002 Apr;7(2):135-46
references.pmid: 12463736
references.citation: Genome Res. 2010 Sep;20(9):1297-303
references.pmid: 20644199
references.citation: Nature. 2003 Oct 23;425(6960):832-6
references.pmid: 14574411
references.citation: Genetics. 2010 Mar;184(3):789-98
references.pmid: 20008575
references.citation: Physiol Genomics. 2010 Mar 3;41(1):21-32
references.pmid: 19996161
references.citation: J Exp Med. 2006 Jan 23;203(1):21-6
references.pmid: 16390940
references.citation: J Anim Sci. 1995 Oct;73(10):3044-50
references.pmid: 8617676
references.citation: Immunology. 1972 Aug;23(2):225-38
references.pmid: 4560742
references.citation: Nat Genet. 2011 May;43(5):491-8
references.pmid: 21478889
references.citation: Genetics. 2009 Jul;182(3):923-6
references.pmid: 19398771
references.citation: J Exp Med. 1999 Oct 4;190(7):915-22
references.pmid: 10510081
references.citation: Immunol Rev. 2005 Aug;206:83-99
references.pmid: 16048543
references.citation: J Dairy Res. 2002 Feb;69(1):27-35
references.pmid: 12047108