title: Rapid and highly sensitive detection of lead ions in drinking water based on a strip immunosensor.
abstract: In this study, we have first developed a rapid and sensitive strip immunosensor based on two heterogeneously-sized gold nanoparticles (Au NPs) probes for the detection of trace lead ions in drinking water. The sensitivity was 4-fold higher than that of the conventional LFA under the optimized conditions. The visual limit of detection (LOD) of the amplified method for qualitative detection lead ions was 2 ng/mL and the LOD for semi-quantitative detection could go down to 0.19 ng/mL using a scanning reader. The method suffered from no interference from other metal ions and could be used to detect trace lead ions in drinking water without sample enrichment. The recovery of the test samples ranged from 96% to 103%. As the detection method could be accomplished within 15 min, this method could be used as a potential tool for preliminary monitoring of lead contamination in drinking water.
authors.affiliation: School of Food Science & Technology, State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, China. khecho@163.com
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Changrui
authors.initials: C
authors.lastname: Xing
authors.forename: Changlong
authors.initials: C
authors.lastname: Hao
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D060766:Drinking Water / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D007477:Ions; D007854:Lead / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D011934:Reagent Strips / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D060766:Drinking Water; D007477:Ions; D011934:Reagent Strips; D007854:Lead
references.citation: Biosens Bioelectron. 2011 Apr 15;26(8):3700-4
references.pmid: 21371875
references.citation: Biosens Bioelectron. 2011 Dec 15;30(1):310-4
references.pmid: 21975341
references.citation: J Sci Food Agric. 2011 Jun;91(8):1392-7
references.pmid: 21337581
references.citation: J Hazard Mater. 2010 Jan 15;173(1-3):669-74
references.pmid: 19765893
references.citation: J Biol Chem. 1996 Nov 1;271(44):27677-85
references.pmid: 8910359
references.citation: Am J Ind Med. 2000 Sep;38(3):295-9
references.pmid: 10940967
references.citation: Bioconjug Chem. 2000 Mar-Apr;11(2):267-77
references.pmid: 10725104
references.citation: Analyst. 2011 Feb 21;136(4):764-8
references.pmid: 21152621
references.citation: Environ Sci Technol. 2002 Mar 1;36(5):1042-7
references.pmid: 11917989
references.citation: Bioconjug Chem. 2004 Jan-Feb;15(1):168-73
references.pmid: 14733597
references.citation: Anal Chem. 2012 Jun 5;84(11):4970-8
references.pmid: 22524145
references.citation: Somatic Cell Genet. 1975 Oct;1(4):397-400
references.pmid: 1242069
references.citation: Biosens Bioelectron. 2010 Apr 15;25(8):1999-2002
references.pmid: 20167468
references.citation: Chem Rev. 2011 May 11;111(5):3433-58
references.pmid: 21395328
references.citation: Chem Commun (Camb). 2011 Apr 14;47(14):4192-4
references.pmid: 21369573
references.citation: Eur J Cell Biol. 1985 Jul;38(1):87-93
references.pmid: 4029177
references.citation: Chem Commun (Camb). 2011 Jul 14;47(26):7437-9
references.pmid: 21584324
references.citation: Biochemistry. 2003 Dec 9;42(48):14173-83
references.pmid: 14640685
references.citation: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Aug;28(8):1021-33
references.pmid: 21728894
references.citation: Environ Pollut. 2010 Jun;158(6):2074-7
references.pmid: 20363540
references.citation: Anal Chim Acta. 2012 Oct 1;745:99-105
references.pmid: 22938612
references.citation: Biosens Bioelectron. 2010 Jul 15;25(11):2534-8
references.pmid: 20444590
references.citation: J Agric Food Chem. 2007 Jun 27;55(13):4993-8
references.pmid: 17547420