title: Characterization of genome-reduced fission yeast strains.
abstract: The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the left arm of chromosome I, 155.4 kb in the right arm of chromosome I, 211.7 kb in the left arm of chromosome II and 121.6 kb in the right arm of chromosome II. The deletions corresponded to a loss of 223 genes of the original ~5100. The quadruple-deletion strain, with a total deletion size of 657.3 kb, showed a decreased ability to uptake glucose and some amino acids in comparison with the parental strain. The strain also showed increased gene expression of the mating pheromone M-factor precursor and the nicotinamide adenine dinucleotide phosphate -specific glutamate dehydrogenase. There was also a 2.7-fold increase in the concentration of cellular adenosine triphosphate, and levels of the heterologous proteins, enhanced green fluorescent protein and secreted human growth hormone were increased by 1.7- and 1.8-fold, respectively. The transcriptome data from this study have been submitted to the Gene Expression Omnibus (GEO: http://www.ncbi.nlm.nih.gov/geo/) under the accession number GSE38620 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=vjkxjewuywgcovc&acc=GSE38620).
authors.affiliation: ASPEX Division, Research Center, Asahi Glass Co, Ltd, Yokohama, Kanagawa 221-8755, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Sasaki
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Kumagai
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Tohda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D002872:Chromosome Deletion; D015870:Gene Expression; D059646:Genome Size; D016681:Genome, Fungal; D005947:Glucose / Q000378:metabolism; D014176:Protein Biosynthesis; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000641:Ammonia; D000255:Adenosine Triphosphate; D005947:Glucose
references.citation: Mol Cell Biol. 2007 Apr;27(8):3154-64
references.pmid: 17261596
references.citation: Appl Microbiol Biotechnol. 2010 Jan;85(3):667-77
references.pmid: 19669754
references.citation: Mol Cell. 2008 Apr 25;30(2):214-26
references.pmid: 18439900
references.citation: Mol Syst Biol. 2006;2:2006.0008
references.pmid: 16738554
references.citation: Nature. 2002 Feb 21;415(6874):871-80
references.pmid: 11859360
references.citation: Glycobiology. 2011 Mar;21(3):340-51
references.pmid: 21098516
references.citation: J Biol Chem. 2012 Nov 9;287(46):38866-75
references.pmid: 22988247
references.citation: Endocrinology. 2003 Dec;144(12):5179-83
references.pmid: 12960015
references.citation: FEMS Microbiol Rev. 2009 Jan;33(1):225-35
references.pmid: 19067748
references.citation: Eukaryot Cell. 2003 Oct;2(5):827-9
references.pmid: 14555464
references.citation: J Proteome Res. 2003 Sep-Oct;2(5):488-94
references.pmid: 14582645
references.citation: FEMS Yeast Res. 2006 Sep;6(6):888-901
references.pmid: 16911511
references.citation: Nucleic Acids Res. 2006 Jan 24;34(2):e11
references.pmid: 16434698
references.citation: Appl Environ Microbiol. 2011 Dec;77(23):8370-81
references.pmid: 21965396
references.citation: Microbiology (Reading). 2003 Aug;149(Pt 8):2107-2117
references.pmid: 12904550
references.citation: J Cell Sci. 2010 Mar 1;123(Pt 5):777-86
references.pmid: 20144990
references.citation: Genetics. 2002 Jul;161(3):1053-63
references.pmid: 12136010
references.citation: Yeast. 1998 Jul;14(10):943-51
references.pmid: 9717240
references.citation: DNA Res. 2008 Apr 30;15(2):73-81
references.pmid: 18334513
references.citation: J Cell Sci. 2006 Nov 1;119(Pt 21):4475-85
references.pmid: 17046992
references.citation: Genes Cells. 2009 May;14(5):539-54
references.pmid: 19371376
references.citation: J Bacteriol. 2000 Apr;182(8):2153-62
references.pmid: 10735857
references.citation: Mol Biosyst. 2008 Feb;4(2):135-47
references.pmid: 18213407
references.citation: J Chromatogr A. 2007 Aug 3;1159(1-2):125-33
references.pmid: 17543971
references.citation: FEMS Yeast Res. 2004 Mar;4(6):649-54
references.pmid: 15040954
references.citation: Exp Cell Res. 1977 Jul;107(2):377-86
references.pmid: 872891
references.citation: Mol Genet Genomics. 2008 May;279(5):441-50
references.pmid: 18219492
references.citation: Eukaryot Cell. 2012 Feb;11(2):159-67
references.pmid: 22140232
references.citation: Cell Struct Funct. 2007;32(2):149-61
references.pmid: 18285637
references.citation: Curr Genet. 1994 Oct;26(4):315-20
references.pmid: 7882425
references.citation: Nature. 2002 Jul 25;418(6896):387-91
references.pmid: 12140549
references.citation: Biotechnol Appl Biochem. 2007 Mar;46(Pt 3):145-6
references.pmid: 17300220
references.citation: Nat Biotechnol. 2010 Jun;28(6):617-623
references.pmid: 20473289
references.citation: Yeast. 2008 Sep;25(9):673-9
references.pmid: 18727152
references.citation: Genetics. 2007 Mar;175(3):1153-62
references.pmid: 17179073
references.citation: Genome Res. 2003 Mar;13(3):399-406
references.pmid: 12618370
references.citation: Appl Microbiol Biotechnol. 2010 Jun;87(2):715-27
references.pmid: 20396879
references.citation: Appl Microbiol Biotechnol. 2007 Jun;75(3):589-97
references.pmid: 17345083
references.citation: Curr Microbiol. 1995 Jun;30(6):367-72
references.pmid: 7773104
references.citation: Cell. 2003 Nov 26;115(5):577-90
references.pmid: 14651849
references.citation: Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4678-83
references.pmid: 12682299
references.citation: DNA Res. 2008 Oct;15(5):277-84
references.pmid: 18753290
references.citation: Nucleic Acids Res. 2006 Jan 10;34(1):78-88
references.pmid: 16407326
references.citation: Nat Rev Microbiol. 2012 Jan 23;10(3):191-202
references.pmid: 22266781
references.citation: Science. 2006 May 19;312(5776):1044-6
references.pmid: 16645050
references.citation: Biotechnol Appl Biochem. 1999 Dec;30(3):235-44
references.pmid: 10574693
references.citation: Biotechnol Appl Biochem. 2009 Jun 22;53(Pt 4):227-35
references.pmid: 19531030